SHM1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	SHM1
Entrez Gene ID	829949
Full Name	serine transhydroxymethyltransferase 1
Synonyms	F20D10.50,F20D10_50,SERINE HYDROXYMETHYLTRANSFERASE 1,SERINE TRANSHYDROXYMETHYLASE,SERINE TRANSHYDROXYMETHYLTRANSFERASE,SHMT1,STM,serine transhydroxymethyltransferase 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_119954.4	NP_195506.1	serine transhydroxymethyltransferase 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Shmt2	NP_082506.1
Rattus norvegicus (Norway rat)	Shmt2	NP_001008323.1
Saccharomyces cerevisiae (baker's yeast)	SHM1	NP_009822.4
Neurospora crassa	NCU05805	XP_960065.1
Pan troglodytes (chimpanzee)	SHMT2	XP_509157.3
Canis lupus familiaris (dog)	SHMT2	XP_849244.1
Kluyveromyces lactis	KLLA0F01210g	XP_455134.1
	SHMT2	XP_001115892.2
Danio rerio (zebrafish)	shmt2	NP_001116846.1
Eremothecium gossypii	AGOS_AEL188W	NP_984673.1
Schizosaccharomyces pombe (fission yeast)	shm2	NP_593668.2
Arabidopsis thaliana (thale cress)	SHM1	NP_195506.1
Homo sapiens (human)	SHMT2	NP_005403.2
Bos taurus (cattle)	SHMT2	NP_001029454.1
Magnaporthe oryzae (rice blast fungus)	MGG_13781	XP_003710851.1
Oryza sativa (rice)	Os03g0738400	NP_001051211.1
Xenopus tropicalis (tropical clawed frog)	shmt2	NP_001007880.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Structural modeling of SHM1, SHM2, and SHM4 presented features that may explain the activity differences between the mitochondrial and cytosolic isozymes.
Title: Folate polyglutamylation eliminates dependence of activity on enzyme concentration in mitochondrial serine hydroxymethyltransferases from Arabidopsis thaliana.

The senescence is initiated in shm1-1 under photorespiratory conditions. Transcription of the senescence marker SAG12 is enhanced in shm1-1.
Title: D-2-hydroxyglutarate metabolism is linked to photorespiration in the shm1-1 mutant.

results suggest that UBP16 is involved in salt tolerance in Arabidopsis by modulating sodium transport activity and repressing cell death at least partially through modulating SMH1stability and activity.
Title: Ubiquitin-specific protease16 modulates salt tolerance in Arabidopsis by regulating Na(+)/H(+) antiport activity and serine hydroxymethyltransferase stability.

SHM1 and SHM2 operate in a redundant manner in one-carbon metabolism of nonphotorespiring cells with a high demand of one-carbon units.
Title: The presequence of Arabidopsis serine hydroxymethyltransferase SHM2 selectively prevents import into mesophyll mitochondria.

SHMT1 interacts with GLU1 and this interaction is necessary for photorespiratory serine hydroxymethyltransferase (SHMT) activity.
Title: Arabidopsis photorespiratory serine hydroxymethyltransferase activity requires the mitochondrial accumulation of ferredoxin-dependent glutamate synthase.

The following SHM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SHM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb17384	NM_119954.4 Latest version!	Arabidopsis thaliana serine transhydroxymethyltransferase 1 (SHM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SHM1 ( NM_119954.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_195506.1 Arabidopsis thaliana serine transhydroxymethyltransferase 1 (SHM1), mRNA.

Price & Availability↑

CloneID	OAb17384
Clone ID Related Accession (Same CDS sequence)	NM_119954.4
Accession Version	NM_119954.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	serine transhydroxymethyltransferase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_119954.3.

ATGGCGATGG CCATGGCTCT TCGAAGGCTT TCTTCTTCAA TTGACAAACC CATTCGTCCT 
CTTATTCGAT CCACTTCATG TTACATGTCT TCTTTGCCCA GTGAAGCTGT TGATGAGAAG 
GAAAGATCTC GTGTCACTTG GCCAAAACAG CTTAACGCAC CTTTAGAGGA GGTTGATCCT 
GAGATTGCTG ACATTATTGA GCATGAGAAA GCTAGACAAT GGAAGGGACT TGAACTTATT 
CCATCTGAGA ACTTCACATC TGTGTCGGTG ATGCAAGCTG TTGGGTCTGT CATGACTAAC 
AAATACAGTG AAGGCTATCC TGGTGCCAGA TACTATGGAG GAAATGAGTA TATAGACATG 
GCAGAAACCT TATGCCAGAA GCGCGCTCTT GAAGCTTTCC GGTTAGATCC TGAAAAGTGG 
GGAGTGAATG TTCAACCTTT GTCTGGATCT CCTGCCAACT TCCATGTGTA CACTGCATTG 
TTAAAGCCTC ATGAAAGAAT CATGGCACTT GATCTTCCTC ATGGTGGTCA TCTTTCTCAT 
GGTTATCAGA CTGACACCAA GAAGATATCA GCTGTGTCTA TCTTCTTTGA AACAATGCCC 
TATAGATTGG ACGAGAGCAC TGGCTACATC GACTACGATC AGATGGAGAA AAGTGCTACT 
CTTTTCAGGC CAAAATTGAT TGTTGCTGGT GCAAGTGCTT ATGCTAGATT GTATGACTAT 
GCCCGCATCA GAAAGGTCTG TAACAAGCAA AAAGCTGTAA TGCTAGCAGA TATGGCACAC 
ATCAGTGGTT TGGTTGCTGC TAATGTAATC CCTTCACCGT TCGACTATGC TGATGTTGTA 
ACCACCACAA CTCACAAGTC ACTTCGTGGA CCCCGTGGAG CCATGATTTT CTTCAGAAAG 
GGTGTTAAGG AAATTAACAA GCAAGGGAAA GAGGTTTTGT ATGATTTTGA AGACAAGATC 
AACCAAGCTG TCTTCCCTGG TCTTCAAGGT GGTCCACACA ACCACACTAT CACAGGACTA 
GCTGTTGCTT TGAAACAGGC AACTACTTCA GAGTACAAAG CATACCAAGA ACAAGTCCTG 
AGTAACAGTG CAAAGTTTGC TCAGACTCTA ATGGAGAGAG GATATGAACT TGTTTCTGGT 
GGAACTGACA ACCATCTGGT TCTAGTGAAT CTAAAGCCCA AGGGAATTGA TGGATCTAGA 
GTTGAGAAAG TGTTGGAAGC TGTTCACATT GCATCCAACA AAAACACTGT TCCTGGAGAT 
GTTTCTGCCA TGGTTCCTGG TGGAATCAGA ATGGGTACTC CTGCTCTCAC TTCCAGAGGC 
TTTGTTGAGG AAGACTTTGC CAAAGTAGCT GAATACTTCG ACAAAGCTGT GACAATAGCT 
CTCAAAGTCA AATCTGAAGC TCAAGGAACC AAGTTGAAGG ATTTCGTGTC AGCAATGGAA 
TCCTCTTCAA CCATCCAATC CGAGATTGCG AAACTGCGCC ATGAAGTCGA GGAATTCGCT 
AAGCAGTTCC CAACAATTGG GTTTGAGAAA GAAACCATGA AGTACAAGAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_195506.1
CDS	276..1829
Translation	MAMAMALRRLSSSIDKPIRPLIRSTSCYMSSLPSEAVDEKERSRVTWPKQLNAPLEEVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAETLCQKRALEAFRLDPEKWGVNVQPLSGSPANFHVYTALLKPHERIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQMEKSATLFRPKLIVAGASAYARLYDYARIRKVCNKQKAVMLADMAHISGLVAANVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRKGVKEINKQGKEVLYDFEDKINQAVFPGLQGGPHNHTITGLAVALKQATTSEYKAYQEQVLSNSAKFAQTLMERGYELVSGGTDNHLVLVNLKPKGIDGSRVEKVLEAVHIASNKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEYFDKAVTIALKVKSEAQGTKLKDFVSAMESSSTIQSEIAKLRHEVEEFAKQFPTIGFEKETMKYKN

Target ORF information:

RefSeq Version	NM_119954.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana serine transhydroxymethyltransferase 1 (SHM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_119954.4

ORF Insert Sequence:

ATGGCGATGG CCATGGCTCT TCGAAGGCTT TCTTCTTCAA TTGACAAACC CATTCGTCCT 
CTTATTCGAT CCACTTCATG TTACATGTCT TCTTTGCCCA GTGAAGCTGT TGATGAGAAG 
GAAAGATCTC GTGTCACTTG GCCAAAACAG CTTAACGCAC CTTTAGAGGA GGTTGATCCT 
GAGATTGCTG ACATTATTGA GCATGAGAAA GCTAGACAAT GGAAGGGACT TGAACTTATT 
CCATCTGAGA ACTTCACATC TGTGTCGGTG ATGCAAGCTG TTGGGTCTGT CATGACTAAC 
AAATACAGTG AAGGCTATCC TGGTGCCAGA TACTATGGAG GAAATGAGTA TATAGACATG 
GCAGAAACCT TATGCCAGAA GCGCGCTCTT GAAGCTTTCC GGTTAGATCC TGAAAAGTGG 
GGAGTGAATG TTCAACCTTT GTCTGGATCT CCTGCCAACT TCCATGTGTA CACTGCATTG 
TTAAAGCCTC ATGAAAGAAT CATGGCACTT GATCTTCCTC ATGGTGGTCA TCTTTCTCAT 
GGTTATCAGA CTGACACCAA GAAGATATCA GCTGTGTCTA TCTTCTTTGA AACAATGCCC 
TATAGATTGG ACGAGAGCAC TGGCTACATC GACTACGATC AGATGGAGAA AAGTGCTACT 
CTTTTCAGGC CAAAATTGAT TGTTGCTGGT GCAAGTGCTT ATGCTAGATT GTATGACTAT 
GCCCGCATCA GAAAGGTCTG TAACAAGCAA AAAGCTGTAA TGCTAGCAGA TATGGCACAC 
ATCAGTGGTT TGGTTGCTGC TAATGTAATC CCTTCACCGT TCGACTATGC TGATGTTGTA 
ACCACCACAA CTCACAAGTC ACTTCGTGGA CCCCGTGGAG CCATGATTTT CTTCAGAAAG 
GGTGTTAAGG AAATTAACAA GCAAGGGAAA GAGGTTTTGT ATGATTTTGA AGACAAGATC 
AACCAAGCTG TCTTCCCTGG TCTTCAAGGT GGTCCACACA ACCACACTAT CACAGGACTA 
GCTGTTGCTT TGAAACAGGC AACTACTTCA GAGTACAAAG CATACCAAGA ACAAGTCCTG 
AGTAACAGTG CAAAGTTTGC TCAGACTCTA ATGGAGAGAG GATATGAACT TGTTTCTGGT 
GGAACTGACA ACCATCTGGT TCTAGTGAAT CTAAAGCCCA AGGGAATTGA TGGATCTAGA 
GTTGAGAAAG TGTTGGAAGC TGTTCACATT GCATCCAACA AAAACACTGT TCCTGGAGAT 
GTTTCTGCCA TGGTTCCTGG TGGAATCAGA ATGGGTACTC CTGCTCTCAC TTCCAGAGGC 
TTTGTTGAGG AAGACTTTGC CAAAGTAGCT GAATACTTCG ACAAAGCTGT GACAATAGCT 
CTCAAAGTCA AATCTGAAGC TCAAGGAACC AAGTTGAAGG ATTTCGTGTC AGCAATGGAA 
TCCTCTTCAA CCATCCAATC CGAGATTGCG AAACTGCGCC ATGAAGTCGA GGAATTCGCT 
AAGCAGTTCC CAACAATTGG GTTTGAGAAA GAAACCATGA AGTACAAGAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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SHM1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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