AT4G31180 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AT4G31180
Entrez Gene ID	829246
Full Name	Class II aminoacyl-tRNA and biotin synthetases superfamily protein
Synonyms	F6E21.100,F6E21_100,IBI1,impaired in BABA-induced disease immunity 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_119268.4	NP_194847.3	Class II aminoacyl-tRNA and biotin synthetases superfamily protein
NM_179227.3	NP_849558.1	Class II aminoacyl-tRNA and biotin synthetases superfamily protein

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DARS	XP_848666.1
Gallus gallus (chicken)	DARS	NP_001006528.1
Caenorhabditis elegans (roundworm)	dars-1	NP_499089.1
Eremothecium gossypii	AGOS_ADL039C	NP_984057.1
Neurospora crassa	NCU07082	XP_960347.1
Oryza sativa (rice)	Os02g0686400	NP_001047770.1
Mus musculus (house mouse)	Dars	NP_803228.2
Rattus norvegicus (Norway rat)	Dars	NP_446251.1
Danio rerio (zebrafish)	dars	NP_001071079.1
Pan troglodytes (chimpanzee)	DARS	XP_001155169.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005576	XP_315584.3
	DARS	XP_001095858.1
Bos taurus (cattle)	DARS	NP_001030257.1
Drosophila melanogaster (fruit fly)	Aats-asp	NP_001260930.1
Saccharomyces cerevisiae (baker's yeast)	DPS1	NP_013083.1
Schizosaccharomyces pombe (fission yeast)	drs1	NP_588352.1
Arabidopsis thaliana (thale cress)	AT4G31180	NP_194847.3
Homo sapiens (human)	DARS	NP_001340.2
Kluyveromyces lactis	KLLA0D03839g	XP_453236.1
Magnaporthe oryzae (rice blast fungus)	MGG_13783	XP_003710853.1
Arabidopsis thaliana (thale cress)	AT4G26870	NP_194417.2
Xenopus tropicalis (tropical clawed frog)	dars	NP_989306.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AT4G31180 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT4G31180 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb16828	NM_179227.3 Latest version!	Arabidopsis thaliana Class II aminoacyl-tRNA and biotin synthetases superfamily protein (AT4G31180), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb16828	NM_119268.4 Latest version!	Arabidopsis thaliana Class II aminoacyl-tRNA and biotin synthetases superfamily protein (AT4G31180), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AT4G31180 ( NM_119268.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_194847.3 Arabidopsis thaliana Class II aminoacyl-tRNA and biotin synthetases superfamily protein (AT4G31180), mRNA.

Price & Availability↑

CloneID	OAb16828
Clone ID Related Accession (Same CDS sequence)	NM_119268.4 , NM_179227.3
Accession Version	NM_119268.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Class II aminoacyl-tRNA and biotin synthetases superfamily protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Apr 18, 2007 this sequence version replaced NM_119268.3.

ATGTCGTCGG AATCTGAAAT CCCGCCGTTG TCGTCATCAA CCGCCGCAGC GGAGGAATCT 
GGAGAGAAGA CCAGCAAGAA AGCGGCTAAG AAGGAAGCTG CCAAGCTAGA GAAGTTAAGA 
CGTCGTCAAG AACAAGAGGA AGCAACGCGT CGAACAGCTT CGATCTCTCT GGAAGAGAAT 
GACGAGTTTT CCAATAACTA CGGCGACGTG ACTCTTACCG AGTTGCAATC GTCGGCGGAT 
CCGAAAGCCG GGAAGTGGAT AGAGGCTGTT GAGGGAAAGG AGTGGACCGA TGTGAGCGAT 
TTGGTGGAAG AGATGTTGGA ATCAGAGGTT CTGATCAGAG GCCGAGTGCA CACGAATCGT 
CCAACGTCTA ACAAATTGGG GTTTGTGGTC TTGAGGGAGA GCGGATCAAC TGTTCAGTGC 
GTGGTTAGCC AATCAGAGAA GACCAAAGTA GGTGCCAACA TGGTTAAATA CCTCAAGCAG 
CTGAGTCGCG AATCCTTTGT CGATGTTATC GGTGTCGTCA CTCTCCCCAA GGAGCCGCTG 
ACGGGAACTA CGCAGCAGGT TGAAATTCAA GTGAGAAAAG TGTACTGCAT CAACAAATCC 
TTGGCCAAAT TACCACTTAG TGTGGAGGAT GCTGCTCGGA GTGAAGCAGA TATCGAAGCA 
TCTCTTCAGA CTCCATCTCC AGCTGCTCGT GTCAATCAGG ATACACGTTT GAACTATAGG 
GTGCTCGACC TCAGAACACC GGCTAATCAA GCCATCTTCC AGCTTCAGTA CGAAGTCGAA 
TATGCCTTCA GAGAAAAATT ACGATTTAAG AATTTTGTTG GAATCCACAC ACCAAAACTG 
ATGGCTGGTA GTAGTGAAGG AGGTTCTGCT GTATTTAGGT TGGAATACAA AGGGCAACCT 
GCTTGTCTAG CTCAGTCTCC TCAGCTCCAC AAGCAAATGG CAATATGTGG TGACTTGCGA 
CGTGTCTTTG AGGTAGGTCC TGTTTTCAGG GCCGAAGACT CCTTCACTCA TAGACACCTG 
TGTGAATTCG TTGGTCTTGA TGTGGAGATG GAGATTCGGA AACACTATTC TGAGATAATG 
GATCTTGTGG ACGAGTTATT TGTGTTTATA TTCACTAGTT TGAATGAGAG GTGCAAAAAA 
GAACTGCAAG CTGTTGGAAA GCAATACCCA TTTGAACCTT TGAAGTTTCT ACCAAAAACA 
TTGAGGTTAA CGTTTGAAGA AGGGGTTCAA ATGCTTAAGG AAGCTGGTGT GGAGGTTGAT 
CCTCTTGGCG ATCTAAACAC TGAATCTGAG AGAAAACTAG GCCAGCTTGT ATTGGAAAAG 
TACAACACTG AGTTCTATAT TCTGCATCGG TATCCTAAGG CAGTTAGGCC TTTCTACACA 
ATGACTTGTG CCGATAATCC TCTTTACAGC AACTCTTTTG ATGTCTTCAT CAGAGGTGAG 
GAGATCATAT CAGGAGCTCA ACGTGTCCAT ATCCCAGAAG TCTTGGAGCA ACGTGCAGGA 
GAATGTGGCA TTGATGTCAA GACAATATCC ACATACATTG ATTCATTCAG GTACGGTGCG 
CCTCTACACG GTGGATTTGG AGTGGGGCTG GAGCGAGTGG TCATGCTTTT CTGTGCACTG 
AACAACATTC GGAAAACATC CCTCTTCCCT CGTGACCCTC AAAGGCTTTC ACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_194847.3
CDS	197..1873
Translation	MSSESEIPPLSSSTAAAEESGEKTSKKAAKKEAAKLEKLRRRQEQEEATRRTASISLEENDEFSNNYGDVTLTELQSSADPKAGKWIEAVEGKEWTDVSDLVEEMLESEVLIRGRVHTNRPTSNKLGFVVLRESGSTVQCVVSQSEKTKVGANMVKYLKQLSRESFVDVIGVVTLPKEPLTGTTQQVEIQVRKVYCINKSLAKLPLSVEDAARSEADIEASLQTPSPAARVNQDTRLNYRVLDLRTPANQAIFQLQYEVEYAFREKLRFKNFVGIHTPKLMAGSSEGGSAVFRLEYKGQPACLAQSPQLHKQMAICGDLRRVFEVGPVFRAEDSFTHRHLCEFVGLDVEMEIRKHYSEIMDLVDELFVFIFTSLNERCKKELQAVGKQYPFEPLKFLPKTLRLTFEEGVQMLKEAGVEVDPLGDLNTESERKLGQLVLEKYNTEFYILHRYPKAVRPFYTMTCADNPLYSNSFDVFIRGEEIISGAQRVHIPEVLEQRAGECGIDVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCALNNIRKTSLFPRDPQRLSP

Target ORF information:

RefSeq Version	NM_119268.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Class II aminoacyl-tRNA and biotin synthetases superfamily protein (AT4G31180), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_119268.4

ORF Insert Sequence:

ATGTCGTCGG AATCTGAAAT CCCGCCGTTG TCGTCATCAA CCGCCGCAGC GGAGGAATCT 
GGAGAGAAGA CCAGCAAGAA AGCGGCTAAG AAGGAAGCTG CCAAGCTAGA GAAGTTAAGA 
CGTCGTCAAG AACAAGAGGA AGCAACGCGT CGAACAGCTT CGATCTCTCT GGAAGAGAAT 
GACGAGTTTT CCAATAACTA CGGCGACGTG ACTCTTACCG AGTTGCAATC GTCGGCGGAT 
CCGAAAGCCG GGAAGTGGAT AGAGGCTGTT GAGGGAAAGG AGTGGACCGA TGTGAGCGAT 
TTGGTGGAAG AGATGTTGGA ATCAGAGGTT CTGATCAGAG GCCGAGTGCA CACGAATCGT 
CCAACGTCTA ACAAATTGGG GTTTGTGGTC TTGAGGGAGA GCGGATCAAC TGTTCAGTGC 
GTGGTTAGCC AATCAGAGAA GACCAAAGTA GGTGCCAACA TGGTTAAATA CCTCAAGCAG 
CTGAGTCGCG AATCCTTTGT CGATGTTATC GGTGTCGTCA CTCTCCCCAA GGAGCCGCTG 
ACGGGAACTA CGCAGCAGGT TGAAATTCAA GTGAGAAAAG TGTACTGCAT CAACAAATCC 
TTGGCCAAAT TACCACTTAG TGTGGAGGAT GCTGCTCGGA GTGAAGCAGA TATCGAAGCA 
TCTCTTCAGA CTCCATCTCC AGCTGCTCGT GTCAATCAGG ATACACGTTT GAACTATAGG 
GTGCTCGACC TCAGAACACC GGCTAATCAA GCCATCTTCC AGCTTCAGTA CGAAGTCGAA 
TATGCCTTCA GAGAAAAATT ACGATTTAAG AATTTTGTTG GAATCCACAC ACCAAAACTG 
ATGGCTGGTA GTAGTGAAGG AGGTTCTGCT GTATTTAGGT TGGAATACAA AGGGCAACCT 
GCTTGTCTAG CTCAGTCTCC TCAGCTCCAC AAGCAAATGG CAATATGTGG TGACTTGCGA 
CGTGTCTTTG AGGTAGGTCC TGTTTTCAGG GCCGAAGACT CCTTCACTCA TAGACACCTG 
TGTGAATTCG TTGGTCTTGA TGTGGAGATG GAGATTCGGA AACACTATTC TGAGATAATG 
GATCTTGTGG ACGAGTTATT TGTGTTTATA TTCACTAGTT TGAATGAGAG GTGCAAAAAA 
GAACTGCAAG CTGTTGGAAA GCAATACCCA TTTGAACCTT TGAAGTTTCT ACCAAAAACA 
TTGAGGTTAA CGTTTGAAGA AGGGGTTCAA ATGCTTAAGG AAGCTGGTGT GGAGGTTGAT 
CCTCTTGGCG ATCTAAACAC TGAATCTGAG AGAAAACTAG GCCAGCTTGT ATTGGAAAAG 
TACAACACTG AGTTCTATAT TCTGCATCGG TATCCTAAGG CAGTTAGGCC TTTCTACACA 
ATGACTTGTG CCGATAATCC TCTTTACAGC AACTCTTTTG ATGTCTTCAT CAGAGGTGAG 
GAGATCATAT CAGGAGCTCA ACGTGTCCAT ATCCCAGAAG TCTTGGAGCA ACGTGCAGGA 
GAATGTGGCA TTGATGTCAA GACAATATCC ACATACATTG ATTCATTCAG GTACGGTGCG 
CCTCTACACG GTGGATTTGG AGTGGGGCTG GAGCGAGTGG TCATGCTTTT CTGTGCACTG 
AACAACATTC GGAAAACATC CCTCTTCCCT CGTGACCCTC AAAGGCTTTC ACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AT4G31180 ( NM_179227.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_849558.1 Arabidopsis thaliana Class II aminoacyl-tRNA and biotin synthetases superfamily protein (AT4G31180), mRNA.

Price & Availability↑

CloneID	OAb16828
Clone ID Related Accession (Same CDS sequence)	NM_119268.4 , NM_179227.3
Accession Version	NM_179227.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Class II aminoacyl-tRNA and biotin synthetases superfamily protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_179227.2.

ATGTCGTCGG AATCTGAAAT CCCGCCGTTG TCGTCATCAA CCGCCGCAGC GGAGGAATCT 
GGAGAGAAGA CCAGCAAGAA AGCGGCTAAG AAGGAAGCTG CCAAGCTAGA GAAGTTAAGA 
CGTCGTCAAG AACAAGAGGA AGCAACGCGT CGAACAGCTT CGATCTCTCT GGAAGAGAAT 
GACGAGTTTT CCAATAACTA CGGCGACGTG ACTCTTACCG AGTTGCAATC GTCGGCGGAT 
CCGAAAGCCG GGAAGTGGAT AGAGGCTGTT GAGGGAAAGG AGTGGACCGA TGTGAGCGAT 
TTGGTGGAAG AGATGTTGGA ATCAGAGGTT CTGATCAGAG GCCGAGTGCA CACGAATCGT 
CCAACGTCTA ACAAATTGGG GTTTGTGGTC TTGAGGGAGA GCGGATCAAC TGTTCAGTGC 
GTGGTTAGCC AATCAGAGAA GACCAAAGTA GGTGCCAACA TGGTTAAATA CCTCAAGCAG 
CTGAGTCGCG AATCCTTTGT CGATGTTATC GGTGTCGTCA CTCTCCCCAA GGAGCCGCTG 
ACGGGAACTA CGCAGCAGGT TGAAATTCAA GTGAGAAAAG TGTACTGCAT CAACAAATCC 
TTGGCCAAAT TACCACTTAG TGTGGAGGAT GCTGCTCGGA GTGAAGCAGA TATCGAAGCA 
TCTCTTCAGA CTCCATCTCC AGCTGCTCGT GTCAATCAGG ATACACGTTT GAACTATAGG 
GTGCTCGACC TCAGAACACC GGCTAATCAA GCCATCTTCC AGCTTCAGTA CGAAGTCGAA 
TATGCCTTCA GAGAAAAATT ACGATTTAAG AATTTTGTTG GAATCCACAC ACCAAAACTG 
ATGGCTGGTA GTAGTGAAGG AGGTTCTGCT GTATTTAGGT TGGAATACAA AGGGCAACCT 
GCTTGTCTAG CTCAGTCTCC TCAGCTCCAC AAGCAAATGG CAATATGTGG TGACTTGCGA 
CGTGTCTTTG AGGTAGGTCC TGTTTTCAGG GCCGAAGACT CCTTCACTCA TAGACACCTG 
TGTGAATTCG TTGGTCTTGA TGTGGAGATG GAGATTCGGA AACACTATTC TGAGATAATG 
GATCTTGTGG ACGAGTTATT TGTGTTTATA TTCACTAGTT TGAATGAGAG GTGCAAAAAA 
GAACTGCAAG CTGTTGGAAA GCAATACCCA TTTGAACCTT TGAAGTTTCT ACCAAAAACA 
TTGAGGTTAA CGTTTGAAGA AGGGGTTCAA ATGCTTAAGG AAGCTGGTGT GGAGGTTGAT 
CCTCTTGGCG ATCTAAACAC TGAATCTGAG AGAAAACTAG GCCAGCTTGT ATTGGAAAAG 
TACAACACTG AGTTCTATAT TCTGCATCGG TATCCTAAGG CAGTTAGGCC TTTCTACACA 
ATGACTTGTG CCGATAATCC TCTTTACAGC AACTCTTTTG ATGTCTTCAT CAGAGGTGAG 
GAGATCATAT CAGGAGCTCA ACGTGTCCAT ATCCCAGAAG TCTTGGAGCA ACGTGCAGGA 
GAATGTGGCA TTGATGTCAA GACAATATCC ACATACATTG ATTCATTCAG GTACGGTGCG 
CCTCTACACG GTGGATTTGG AGTGGGGCTG GAGCGAGTGG TCATGCTTTT CTGTGCACTG 
AACAACATTC GGAAAACATC CCTCTTCCCT CGTGACCCTC AAAGGCTTTC ACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_849558.1
CDS	351..2027
Translation	MSSESEIPPLSSSTAAAEESGEKTSKKAAKKEAAKLEKLRRRQEQEEATRRTASISLEENDEFSNNYGDVTLTELQSSADPKAGKWIEAVEGKEWTDVSDLVEEMLESEVLIRGRVHTNRPTSNKLGFVVLRESGSTVQCVVSQSEKTKVGANMVKYLKQLSRESFVDVIGVVTLPKEPLTGTTQQVEIQVRKVYCINKSLAKLPLSVEDAARSEADIEASLQTPSPAARVNQDTRLNYRVLDLRTPANQAIFQLQYEVEYAFREKLRFKNFVGIHTPKLMAGSSEGGSAVFRLEYKGQPACLAQSPQLHKQMAICGDLRRVFEVGPVFRAEDSFTHRHLCEFVGLDVEMEIRKHYSEIMDLVDELFVFIFTSLNERCKKELQAVGKQYPFEPLKFLPKTLRLTFEEGVQMLKEAGVEVDPLGDLNTESERKLGQLVLEKYNTEFYILHRYPKAVRPFYTMTCADNPLYSNSFDVFIRGEEIISGAQRVHIPEVLEQRAGECGIDVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCALNNIRKTSLFPRDPQRLSP

Target ORF information:

RefSeq Version	NM_179227.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Class II aminoacyl-tRNA and biotin synthetases superfamily protein (AT4G31180), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_179227.3

ORF Insert Sequence:

ATGTCGTCGG AATCTGAAAT CCCGCCGTTG TCGTCATCAA CCGCCGCAGC GGAGGAATCT 
GGAGAGAAGA CCAGCAAGAA AGCGGCTAAG AAGGAAGCTG CCAAGCTAGA GAAGTTAAGA 
CGTCGTCAAG AACAAGAGGA AGCAACGCGT CGAACAGCTT CGATCTCTCT GGAAGAGAAT 
GACGAGTTTT CCAATAACTA CGGCGACGTG ACTCTTACCG AGTTGCAATC GTCGGCGGAT 
CCGAAAGCCG GGAAGTGGAT AGAGGCTGTT GAGGGAAAGG AGTGGACCGA TGTGAGCGAT 
TTGGTGGAAG AGATGTTGGA ATCAGAGGTT CTGATCAGAG GCCGAGTGCA CACGAATCGT 
CCAACGTCTA ACAAATTGGG GTTTGTGGTC TTGAGGGAGA GCGGATCAAC TGTTCAGTGC 
GTGGTTAGCC AATCAGAGAA GACCAAAGTA GGTGCCAACA TGGTTAAATA CCTCAAGCAG 
CTGAGTCGCG AATCCTTTGT CGATGTTATC GGTGTCGTCA CTCTCCCCAA GGAGCCGCTG 
ACGGGAACTA CGCAGCAGGT TGAAATTCAA GTGAGAAAAG TGTACTGCAT CAACAAATCC 
TTGGCCAAAT TACCACTTAG TGTGGAGGAT GCTGCTCGGA GTGAAGCAGA TATCGAAGCA 
TCTCTTCAGA CTCCATCTCC AGCTGCTCGT GTCAATCAGG ATACACGTTT GAACTATAGG 
GTGCTCGACC TCAGAACACC GGCTAATCAA GCCATCTTCC AGCTTCAGTA CGAAGTCGAA 
TATGCCTTCA GAGAAAAATT ACGATTTAAG AATTTTGTTG GAATCCACAC ACCAAAACTG 
ATGGCTGGTA GTAGTGAAGG AGGTTCTGCT GTATTTAGGT TGGAATACAA AGGGCAACCT 
GCTTGTCTAG CTCAGTCTCC TCAGCTCCAC AAGCAAATGG CAATATGTGG TGACTTGCGA 
CGTGTCTTTG AGGTAGGTCC TGTTTTCAGG GCCGAAGACT CCTTCACTCA TAGACACCTG 
TGTGAATTCG TTGGTCTTGA TGTGGAGATG GAGATTCGGA AACACTATTC TGAGATAATG 
GATCTTGTGG ACGAGTTATT TGTGTTTATA TTCACTAGTT TGAATGAGAG GTGCAAAAAA 
GAACTGCAAG CTGTTGGAAA GCAATACCCA TTTGAACCTT TGAAGTTTCT ACCAAAAACA 
TTGAGGTTAA CGTTTGAAGA AGGGGTTCAA ATGCTTAAGG AAGCTGGTGT GGAGGTTGAT 
CCTCTTGGCG ATCTAAACAC TGAATCTGAG AGAAAACTAG GCCAGCTTGT ATTGGAAAAG 
TACAACACTG AGTTCTATAT TCTGCATCGG TATCCTAAGG CAGTTAGGCC TTTCTACACA 
ATGACTTGTG CCGATAATCC TCTTTACAGC AACTCTTTTG ATGTCTTCAT CAGAGGTGAG 
GAGATCATAT CAGGAGCTCA ACGTGTCCAT ATCCCAGAAG TCTTGGAGCA ACGTGCAGGA 
GAATGTGGCA TTGATGTCAA GACAATATCC ACATACATTG ATTCATTCAG GTACGGTGCG 
CCTCTACACG GTGGATTTGG AGTGGGGCTG GAGCGAGTGG TCATGCTTTT CTGTGCACTG 
AACAACATTC GGAAAACATC CCTCTTCCCT CGTGACCCTC AAAGGCTTTC ACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
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AT4G31180 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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