HA2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	HA2
Entrez Gene ID	829142
Full Name	H[+]-ATPase 2
Synonyms	AHA2,F9N11.40,F9N11_40,H(+)-ATPase 2,P-TYPE H(+)-ATPASE ISOFORM 2,PLASMA MEMBRANE PROTON ATPASE 2,PMA2
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001203941.2	NP_001190870.1	H[+]-ATPase 2
NM_119165.4	NP_194748.1	H[+]-ATPase 2

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_04994	XP_003712494.1
Arabidopsis thaliana (thale cress)	HA1	NP_179486.1
Arabidopsis thaliana (thale cress)	HA2	NP_194748.1
Oryza sativa (rice)	Os04g0656100	NP_001054118.1
Arabidopsis thaliana (thale cress)	HA3	NP_001190559.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the PM proton pump AHA2 (Arabidopsis plasma membrane H(+) -ATPase isoform 2) is important for root growth and development during different nitrogen regimes.
Title: The plasma membrane H(+) -ATPase AHA2 contributes to the root architecture in response to different nitrogen supply.

AHA2 is activated by lysophospholipids directly via a mechanism involving N-and C- terminal domains.
Title: Specific Activation of the Plant P-type Plasma Membrane H+-ATPase by Lysophospholipids Depends on the Autoinhibitory N- and C-terminal Domains.

The major plasma membrane proton pumps in Arabidopsis, AHA1 and AHA2, interact directly in vitro and in planta with PSY1R, a receptor kinase of the plasma membrane that serves as a receptor for the peptide growth hormone PSY1.
Title: Receptor kinase-mediated control of primary active proton pumping at the plasma membrane.

analysis of Arabidopsis thaliana plasma membrane H(+)-ATPase isoform 2 reconstituted into soluble nanoscale lipid bilayers, also termed nanodiscs
Title: Active plasma membrane P-type H+-ATPase reconstituted into nanodiscs is a monomer.

Asn-106 is important for closure of the proton entrance pathway prior to proton translocation across the membrane in AHA2
Title: A conserved asparagine in a P-type proton pump is required for efficient gating of protons.

The following HA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb16729	NM_119165.4 Latest version!	Arabidopsis thaliana H[+]-ATPase 2 (HA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb30884	NM_001203941.2 Latest version!	Arabidopsis thaliana H[+]-ATPase 2 (HA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HA2 ( NM_119165.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_194748.1 Arabidopsis thaliana H[+]-ATPase 2 (HA2), mRNA.

Price & Availability↑

CloneID	OAb16729
Clone ID Related Accession (Same CDS sequence)	NM_119165.4
Accession Version	NM_119165.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	H[+]-ATPase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_119165.3.

ATGTCGAGTC TCGAAGATAT CAAGAACGAG ACTGTTGATC TGGAAAAAAT TCCGATTGAG 
GAAGTTTTCC AGCAGCTAAA ATGTTCAAGG GAAGGATTGA CAACGCAGGA AGGGGAGGAC 
AGGATTCAGA TCTTTGGCCC CAACAAGCTC GAAGAGAAAA AGGAAAGCAA ACTTCTGAAG 
TTTTTGGGGT TTATGTGGAA TCCACTTTCA TGGGTCATGG AAATGGCTGC AATCATGGCC 
ATTGCTTTGG CCAACGGTGA TGGTAGGCCT CCGGATTGGC AGGATTTTGT TGGTATTATC 
TGTCTGTTGG TTATCAACTC TACCATCAGT TTTATCGAAG AAAACAATGC TGGTAATGCT 
GCTGCTGCTC TTATGGCTGG TCTTGCTCCT AAAACCAAGG TTCTTAGGGA TGGAAAGTGG 
AGTGAACAAG AAGCTGCTAT TCTTGTCCCA GGAGATATTG TTAGCATTAA ATTAGGAGAC 
ATTATCCCAG CTGATGCCCG TCTACTTGAA GGTGATCCTT TAAAGGTTGA CCAATCTGCT 
CTAACTGGAG AGTCCCTTCC TGTAACCAAG CACCCGGGTC AAGAAGTTTT CTCTGGTTCA 
ACCTGCAAAC AAGGAGAAAT CGAGGCGGTT GTTATTGCCA CTGGGGTTCA TACCTTCTTC 
GGTAAAGCTG CTCACCTTGT GGACAGCACT AACCAAGTTG GACATTTCCA GAAGGTTCTT 
ACAGCCATTG GGAACTTCTG TATCTGTTCC ATTGCTATCG GTATGGTGAT TGAGATCATC 
GTCATGTATC CGATCCAACG CCGAAAGTAC AGAGATGGAA TTGACAACCT TTTGGTCCTC 
TTGATCGGTG GTATCCCCAT TGCTATGCCT ACAGTCTTGT CCGTGACCAT GGCTATTGGG 
TCTCACAGGT TGTCTCAGCA AGGTGCCATC ACCAAGCGTA TGACTGCCAT TGAAGAGATG 
GCAGGAATGG ATGTCCTGTG CAGTGACAAA ACCGGGACAC TAACCCTCAA CAAATTGAGT 
GTGGACAAAA ACTTGGTCGA GGTTTTCTGC AAGGGTGTGG AGAAAGATCA AGTCCTATTA 
TTTGCAGCTA TGGCTTCCAG GGTTGAGAAC CAGGATGCCA TTGATGCAGC CATGGTTGGG 
ATGCTTGCTG ATCCAAAGGA GGCTAGAGCT GGAATCAGGG AAGTTCACTT CCTTCCATTC 
AACCCTGTGG ATAAGAGAAC TGCTTTGACT TACATTGACG GCAGTGGTAA CTGGCACAGA 
GTCAGTAAAG GTGCTCCTGA GCAGATCCTC GAACTTGCCA AAGCCAGCAA TGATCTTAGC 
AAGAAGGTGC TCTCCATTAT TGACAAGTAT GCTGAGCGTG GTCTTAGGTC GTTGGCTGTT 
GCTCGCCAGG TGGTGCCAGA GAAAACAAAG GAAAGCCCAG GTGCGCCATG GGAATTTGTT 
GGCTTGTTGC CACTTTTTGA TCCCCCAAGA CATGACAGTG CTGAAACAAT TCGACGGGCT 
TTGAATCTTG GTGTTAACGT CAAGATGATC ACTGGTGACC AACTTGCTAT TGGTAAGGAA 
ACTGGTCGCA GACTTGGAAT GGGAACAAAC ATGTATCCAT CTTCGGCTCT TCTTGGTACA 
CACAAAGACG CAAACCTCGC ATCCATTCCT GTTGAGGAGT TGATTGAAAA GGCTGATGGA 
TTTGCCGGAG TCTTCCCAGA GCACAAATAC GAAATTGTGA AAAAGTTGCA GGAGAGGAAG 
CATATTGTTG GAATGACTGG TGATGGTGTC AATGATGCCC CTGCTCTAAA GAAAGCTGAT 
ATCGGTATTG CTGTTGCTGA TGCTACAGAT GCTGCTCGTG GTGCTTCAGA TATCGTGCTC 
ACTGAGCCTG GACTCAGCGT TATTATCAGT GCTGTTCTCA CCAGCAGAGC TATTTTCCAG 
AGAATGAAGA ACTATACTAT CTATGCAGTC TCAATCACCA TCCGTATTGT GTTTGGTTTC 
ATGCTTATTG CTTTGATATG GGAATTTGAC TTCTCAGCCT TCATGGTTCT GATCATTGCC 
ATTCTTAACG ACGGTACCAT CATGACAATC TCAAAGGACA GAGTTAAGCC ATCTCCCACA 
CCTGATAGCT GGAAACTTAA AGAAATTTTT GCTACTGGAG TCGTTCTAGG AGGCTACCAG 
GCCATCATGA CTGTTATTTT CTTCTGGGCG GCGCACAAGA CTGACTTTTT CTCGGACACA 
TTCGGTGTGA GGTCCATTAG GGACAATAAC CACGAGCTAA TGGGTGCGGT GTACTTACAA 
GTTAGTATCA TTAGTCAAGC TCTGATCTTC GTCACAAGAT CAAGGAGTTG GTCTTTTGTT 
GAACGTCCTG GAGCATTGCT GATGATTGCT TTCCTCATTG CACAACTGAT TGCTACTTTG 
ATTGCGGTTT ACGCCAACTG GGAATTTGCA AAGATTAGGG GTATTGGATG GGGATGGGCT 
GGTGTGATCT GGCTATACAG TATTGTCACA TACTTCCCAT TGGACGTTTT CAAGTTTGCC 
ATTCGATACA TCTTGAGCGG AAAGGCGTGG CTCAACTTGT TTGAGAACAA GACGGCTTTC 
ACGATGAAGA AAGATTACGG AAAAGAAGAG AGAGAGGCTC AATGGGCACT TGCTCAAAGG 
ACACTTCACG GTTTACAGCC AAAAGAAGCT GTTAACATCT TCCCTGAGAA AGGAAGTTAC 
AGAGAATTGT CTGAGATCGC TGAGCAAGCT AAGAGAAGAG CTGAGATCGC TAGGCTTAGG 
GAGCTGCACA CACTCAAGGG ACATGTGGAA TCAGTCGTGA AGCTAAAGGG CTTGGACATT 
GAAACTCCCA GTCACTACAC TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_194748.1
CDS	328..3174
Translation	MSSLEDIKNETVDLEKIPIEEVFQQLKCSREGLTTQEGEDRIQIFGPNKLEEKKESKLLKFLGFMWNPLSWVMEMAAIMAIALANGDGRPPDWQDFVGIICLLVINSTISFIEENNAGNAAAALMAGLAPKTKVLRDGKWSEQEAAILVPGDIVSIKLGDIIPADARLLEGDPLKVDQSALTGESLPVTKHPGQEVFSGSTCKQGEIEAVVIATGVHTFFGKAAHLVDSTNQVGHFQKVLTAIGNFCICSIAIGMVIEIIVMYPIQRRKYRDGIDNLLVLLIGGIPIAMPTVLSVTMAIGSHRLSQQGAITKRMTAIEEMAGMDVLCSDKTGTLTLNKLSVDKNLVEVFCKGVEKDQVLLFAAMASRVENQDAIDAAMVGMLADPKEARAGIREVHFLPFNPVDKRTALTYIDGSGNWHRVSKGAPEQILELAKASNDLSKKVLSIIDKYAERGLRSLAVARQVVPEKTKESPGAPWEFVGLLPLFDPPRHDSAETIRRALNLGVNVKMITGDQLAIGKETGRRLGMGTNMYPSSALLGTHKDANLASIPVEELIEKADGFAGVFPEHKYEIVKKLQERKHIVGMTGDGVNDAPALKKADIGIAVADATDAARGASDIVLTEPGLSVIISAVLTSRAIFQRMKNYTIYAVSITIRIVFGFMLIALIWEFDFSAFMVLIIAILNDGTIMTISKDRVKPSPTPDSWKLKEIFATGVVLGGYQAIMTVIFFWAAHKTDFFSDTFGVRSIRDNNHELMGAVYLQVSIISQALIFVTRSRSWSFVERPGALLMIAFLIAQLIATLIAVYANWEFAKIRGIGWGWAGVIWLYSIVTYFPLDVFKFAIRYILSGKAWLNLFENKTAFTMKKDYGKEEREAQWALAQRTLHGLQPKEAVNIFPEKGSYRELSEIAEQAKRRAEIARLRELHTLKGHVESVVKLKGLDIETPSHYTV

Target ORF information:

RefSeq Version	NM_119165.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana H[+]-ATPase 2 (HA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_119165.4

ORF Insert Sequence:

ATGTCGAGTC TCGAAGATAT CAAGAACGAG ACTGTTGATC TGGAAAAAAT TCCGATTGAG 
GAAGTTTTCC AGCAGCTAAA ATGTTCAAGG GAAGGATTGA CAACGCAGGA AGGGGAGGAC 
AGGATTCAGA TCTTTGGCCC CAACAAGCTC GAAGAGAAAA AGGAAAGCAA ACTTCTGAAG 
TTTTTGGGGT TTATGTGGAA TCCACTTTCA TGGGTCATGG AAATGGCTGC AATCATGGCC 
ATTGCTTTGG CCAACGGTGA TGGTAGGCCT CCGGATTGGC AGGATTTTGT TGGTATTATC 
TGTCTGTTGG TTATCAACTC TACCATCAGT TTTATCGAAG AAAACAATGC TGGTAATGCT 
GCTGCTGCTC TTATGGCTGG TCTTGCTCCT AAAACCAAGG TTCTTAGGGA TGGAAAGTGG 
AGTGAACAAG AAGCTGCTAT TCTTGTCCCA GGAGATATTG TTAGCATTAA ATTAGGAGAC 
ATTATCCCAG CTGATGCCCG TCTACTTGAA GGTGATCCTT TAAAGGTTGA CCAATCTGCT 
CTAACTGGAG AGTCCCTTCC TGTAACCAAG CACCCGGGTC AAGAAGTTTT CTCTGGTTCA 
ACCTGCAAAC AAGGAGAAAT CGAGGCGGTT GTTATTGCCA CTGGGGTTCA TACCTTCTTC 
GGTAAAGCTG CTCACCTTGT GGACAGCACT AACCAAGTTG GACATTTCCA GAAGGTTCTT 
ACAGCCATTG GGAACTTCTG TATCTGTTCC ATTGCTATCG GTATGGTGAT TGAGATCATC 
GTCATGTATC CGATCCAACG CCGAAAGTAC AGAGATGGAA TTGACAACCT TTTGGTCCTC 
TTGATCGGTG GTATCCCCAT TGCTATGCCT ACAGTCTTGT CCGTGACCAT GGCTATTGGG 
TCTCACAGGT TGTCTCAGCA AGGTGCCATC ACCAAGCGTA TGACTGCCAT TGAAGAGATG 
GCAGGAATGG ATGTCCTGTG CAGTGACAAA ACCGGGACAC TAACCCTCAA CAAATTGAGT 
GTGGACAAAA ACTTGGTCGA GGTTTTCTGC AAGGGTGTGG AGAAAGATCA AGTCCTATTA 
TTTGCAGCTA TGGCTTCCAG GGTTGAGAAC CAGGATGCCA TTGATGCAGC CATGGTTGGG 
ATGCTTGCTG ATCCAAAGGA GGCTAGAGCT GGAATCAGGG AAGTTCACTT CCTTCCATTC 
AACCCTGTGG ATAAGAGAAC TGCTTTGACT TACATTGACG GCAGTGGTAA CTGGCACAGA 
GTCAGTAAAG GTGCTCCTGA GCAGATCCTC GAACTTGCCA AAGCCAGCAA TGATCTTAGC 
AAGAAGGTGC TCTCCATTAT TGACAAGTAT GCTGAGCGTG GTCTTAGGTC GTTGGCTGTT 
GCTCGCCAGG TGGTGCCAGA GAAAACAAAG GAAAGCCCAG GTGCGCCATG GGAATTTGTT 
GGCTTGTTGC CACTTTTTGA TCCCCCAAGA CATGACAGTG CTGAAACAAT TCGACGGGCT 
TTGAATCTTG GTGTTAACGT CAAGATGATC ACTGGTGACC AACTTGCTAT TGGTAAGGAA 
ACTGGTCGCA GACTTGGAAT GGGAACAAAC ATGTATCCAT CTTCGGCTCT TCTTGGTACA 
CACAAAGACG CAAACCTCGC ATCCATTCCT GTTGAGGAGT TGATTGAAAA GGCTGATGGA 
TTTGCCGGAG TCTTCCCAGA GCACAAATAC GAAATTGTGA AAAAGTTGCA GGAGAGGAAG 
CATATTGTTG GAATGACTGG TGATGGTGTC AATGATGCCC CTGCTCTAAA GAAAGCTGAT 
ATCGGTATTG CTGTTGCTGA TGCTACAGAT GCTGCTCGTG GTGCTTCAGA TATCGTGCTC 
ACTGAGCCTG GACTCAGCGT TATTATCAGT GCTGTTCTCA CCAGCAGAGC TATTTTCCAG 
AGAATGAAGA ACTATACTAT CTATGCAGTC TCAATCACCA TCCGTATTGT GTTTGGTTTC 
ATGCTTATTG CTTTGATATG GGAATTTGAC TTCTCAGCCT TCATGGTTCT GATCATTGCC 
ATTCTTAACG ACGGTACCAT CATGACAATC TCAAAGGACA GAGTTAAGCC ATCTCCCACA 
CCTGATAGCT GGAAACTTAA AGAAATTTTT GCTACTGGAG TCGTTCTAGG AGGCTACCAG 
GCCATCATGA CTGTTATTTT CTTCTGGGCG GCGCACAAGA CTGACTTTTT CTCGGACACA 
TTCGGTGTGA GGTCCATTAG GGACAATAAC CACGAGCTAA TGGGTGCGGT GTACTTACAA 
GTTAGTATCA TTAGTCAAGC TCTGATCTTC GTCACAAGAT CAAGGAGTTG GTCTTTTGTT 
GAACGTCCTG GAGCATTGCT GATGATTGCT TTCCTCATTG CACAACTGAT TGCTACTTTG 
ATTGCGGTTT ACGCCAACTG GGAATTTGCA AAGATTAGGG GTATTGGATG GGGATGGGCT 
GGTGTGATCT GGCTATACAG TATTGTCACA TACTTCCCAT TGGACGTTTT CAAGTTTGCC 
ATTCGATACA TCTTGAGCGG AAAGGCGTGG CTCAACTTGT TTGAGAACAA GACGGCTTTC 
ACGATGAAGA AAGATTACGG AAAAGAAGAG AGAGAGGCTC AATGGGCACT TGCTCAAAGG 
ACACTTCACG GTTTACAGCC AAAAGAAGCT GTTAACATCT TCCCTGAGAA AGGAAGTTAC 
AGAGAATTGT CTGAGATCGC TGAGCAAGCT AAGAGAAGAG CTGAGATCGC TAGGCTTAGG 
GAGCTGCACA CACTCAAGGG ACATGTGGAA TCAGTCGTGA AGCTAAAGGG CTTGGACATT 
GAAACTCCCA GTCACTACAC TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HA2 ( NM_001203941.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001190870.1 Arabidopsis thaliana H[+]-ATPase 2 (HA2), mRNA.

Price & Availability↑

CloneID	OAb30884
Clone ID Related Accession (Same CDS sequence)	NM_001203941.2
Accession Version	NM_001203941.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	H[+]-ATPase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_001203941.1.

ATGTCGAGTC TCGAAGATAT CAAGAACGAG ACTGTTGATC TGGAAAAAAT TCCGATTGAG 
GAAGTTTTCC AGCAGCTAAA ATGTTCAAGG GAAGGATTGA CAACGCAGGA AGGGGAGGAC 
AGGATTCAGA TCTTTGGCCC CAACAAGCTC GAAGAGAAAA AGGAAAGCAA ACTTCTGAAG 
TTTTTGGGGT TTATGTGGAA TCCACTTTCA TGGGTCATGG AAATGGCTGC AATCATGGCC 
ATTGCTTTGG CCAACGGTGA TGGTAGGCCT CCGGATTGGC AGGATTTTGT TGGTATTATC 
TGTCTGTTGG TTATCAACTC TACCATCAGT TTTATCGAAG AAAACAATGC TGGTAATGCT 
GCTGCTGCTC TTATGGCTGG TCTTGCTCCT AAAACCAAGG TTCTTAGGGA TGGAAAGTGG 
AGTGAACAAG AAGCTGCTAT TCTTGTCCCA GGAGATATTG TTAGCATTAA ATTAGGAGAC 
ATTATCCCAG CTGATGCCCG TCTACTTGAA GGTGATCCTT TAAAGGTTGA CCAATCTGCT 
CTAACTGGAG AGTCCCTTCC TGTAACCAAG CACCCGGGTC AAGAAGTTTT CTCTGGTTCA 
ACCTGCAAAC AAGGAGAAAT CGAGGCGGTT GTTATTGCCA CTGGGGTTCA TACCTTCTTC 
GGTAAAGCTG CTCACCTTGT GGACAGCACT AACCAAGTTG GACATTTCCA GAAGGTTCTT 
ACAGCCATTG GGAACTTCTG TATCTGTTCC ATTGCTATCG GTATGGTGAT TGAGATCATC 
GTCATGTATC CGATCCAACG CCGAAAGTAC AGAGATGGAA TTGACAACCT TTTGGTCCTC 
TTGATCGGTG GTATCCCCAT TGCTATGCCT ACAGTCTTGT CCGTGACCAT GGCTATTGGG 
TCTCACAGGT TGTCTCAGCA AGGTGCCATC ACCAAGCGTA TGACTGCCAT TGAAGAGATG 
GCAGGAATGG ATGTCCTGTG CAGTGACAAA ACCGGGACAC TAACCCTCAA CAAATTGAGT 
GTGGACAAAA ACTTGGTCGA GGTTTTCTGC AAGGGTGTGG AGAAAGATCA AGTCCTATTA 
TTTGCAGCTA TGGCTTCCAG GGTTGAGAAC CAGGATGCCA TTGATGCAGC CATGGTTGGG 
ATGCTTGCTG ATCCAAAGGA GGCTAGAGCT GGAATCAGGG AAGTTCACTT CCTTCCATTC 
AACCCTGTGG ATAAGAGAAC TGCTTTGACT TACATTGACG GCAGTGGTAA CTGGCACAGA 
GTCAGTAAAG GTGCTCCTGA GCAGATCCTC GAACTTGCCA AAGCCAGCAA TGATCTTAGC 
AAGAAGGTGC TCTCCATTAT TGACAAGTAT GCTGAGCGTG GTCTTAGGTC GTTGGCTGTT 
GCTCGCCAGG TGGTGCCAGA GAAAACAAAG GAAAGCCCAG GTGCGCCATG GGAATTTGTT 
GGCTTGTTGC CACTTTTTGA TCCCCCAAGA CATGACAGTG CTGAAACAAT TCGACGGGCT 
TTGAATCTTG GTGTTAACGT CAAGATGATC ACTGGTGACC AACTTGCTAT TGGTAAGGAA 
ACTGGTCGCA GACTTGGAAT GGGAACAAAC ATGTATCCAT CTTCGGCTCT TCTTGGTACA 
CACAAAGACG CAAACCTCGC ATCCATTCCT GTTGAGGAGT TGATTGAAAA GGCTGATGGA 
TTTGCCGGAG TCTTCCCAGG TTATAATCTG CTTATTTATT GTTTGGATTA TAAACCTCAC 
TATATGTTCA TTGCAAAGGT GGTGATGTTA GTTCTAAGCT TTGTTTTTTT TATTGCAGAG 
CACAAATACG AAATTGTGAA AAAGTTGCAG GAGAGGAAGC ATATTGTTGG AATGACTGGT 
GATGGTGTCA ATGATGCCCC TGCTCTAAAG AAAGCTGATA TCGGTATTGC TGTTGCTGAT 
GCTACAGATG CTGCTCGTGG TGCTTCAGAT ATCGTGCTCA CTGAGCCTGG ACTCAGCGTT 
ATTATCAGTG CTGTTCTCAC CAGCAGAGCT ATTTTCCAGA GAATGAAGAA CTATACTATC 
TATGCAGTCT CAATCACCAT CCGTATTGTG TTTGGTTTCA TGCTTATTGC TTTGATATGG 
GAATTTGACT TCTCAGCCTT CATGGTTCTG ATCATTGCCA TTCTTAACGA CGGTACCATC 
ATGACAATCT CAAAGGACAG AGTTAAGCCA TCTCCCACAC CTGATAGCTG GAAACTTAAA 
GAAATTTTTG CTACTGGAGT CGTTCTAGGA GGCTACCAGG CCATCATGAC TGTTATTTTC 
TTCTGGGCGG CGCACAAGAC TGACTTTTTC TCGGACACAT TCGGTGTGAG GTCCATTAGG 
GACAATAACC ACGAGCTAAT GGGTGCGGTG TACTTACAAG TTAGTATCAT TAGTCAAGCT 
CTGATCTTCG TCACAAGATC AAGGAGTTGG TCTTTTGTTG AACGTCCTGG AGCATTGCTG 
ATGATTGCTT TCCTCATTGC ACAACTGATT GCTACTTTGA TTGCGGTTTA CGCCAACTGG 
GAATTTGCAA AGATTAGGGG TATTGGATGG GGATGGGCTG GTGTGATCTG GCTATACAGT 
ATTGTCACAT ACTTCCCATT GGACGTTTTC AAGTTTGCCA TTCGATACAT CTTGAGCGGA 
AAGGCGTGGC TCAACTTGTT TGAGAACAAG ACGGCTTTCA CGATGAAGAA AGATTACGGA 
AAAGAAGAGA GAGAGGCTCA ATGGGCACTT GCTCAAAGGA CACTTCACGG TTTACAGCCA 
AAAGAAGCTG TTAACATCTT CCCTGAGAAA GGAAGTTACA GAGAATTGTC TGAGATCGCT 
GAGCAAGCTA AGAGAAGAGC TGAGATCGCT AGGCTTAGGG AGCTGCACAC ACTCAAGGGA 
CATGTGGAAT CAGTCGTGAA GCTAAAGGGC TTGGACATTG AAACTCCCAG TCACTACACT 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001190870.1
CDS	139..3084
Translation	MSSLEDIKNETVDLEKIPIEEVFQQLKCSREGLTTQEGEDRIQIFGPNKLEEKKESKLLKFLGFMWNPLSWVMEMAAIMAIALANGDGRPPDWQDFVGIICLLVINSTISFIEENNAGNAAAALMAGLAPKTKVLRDGKWSEQEAAILVPGDIVSIKLGDIIPADARLLEGDPLKVDQSALTGESLPVTKHPGQEVFSGSTCKQGEIEAVVIATGVHTFFGKAAHLVDSTNQVGHFQKVLTAIGNFCICSIAIGMVIEIIVMYPIQRRKYRDGIDNLLVLLIGGIPIAMPTVLSVTMAIGSHRLSQQGAITKRMTAIEEMAGMDVLCSDKTGTLTLNKLSVDKNLVEVFCKGVEKDQVLLFAAMASRVENQDAIDAAMVGMLADPKEARAGIREVHFLPFNPVDKRTALTYIDGSGNWHRVSKGAPEQILELAKASNDLSKKVLSIIDKYAERGLRSLAVARQVVPEKTKESPGAPWEFVGLLPLFDPPRHDSAETIRRALNLGVNVKMITGDQLAIGKETGRRLGMGTNMYPSSALLGTHKDANLASIPVEELIEKADGFAGVFPGYNLLIYCLDYKPHYMFIAKVVMLVLSFVFFIAEHKYEIVKKLQERKHIVGMTGDGVNDAPALKKADIGIAVADATDAARGASDIVLTEPGLSVIISAVLTSRAIFQRMKNYTIYAVSITIRIVFGFMLIALIWEFDFSAFMVLIIAILNDGTIMTISKDRVKPSPTPDSWKLKEIFATGVVLGGYQAIMTVIFFWAAHKTDFFSDTFGVRSIRDNNHELMGAVYLQVSIISQALIFVTRSRSWSFVERPGALLMIAFLIAQLIATLIAVYANWEFAKIRGIGWGWAGVIWLYSIVTYFPLDVFKFAIRYILSGKAWLNLFENKTAFTMKKDYGKEEREAQWALAQRTLHGLQPKEAVNIFPEKGSYRELSEIAEQAKRRAEIARLRELHTLKGHVESVVKLKGLDIETPSHYTV

Target ORF information:

RefSeq Version	NM_001203941.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana H[+]-ATPase 2 (HA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001203941.2

ORF Insert Sequence:

ATGTCGAGTC TCGAAGATAT CAAGAACGAG ACTGTTGATC TGGAAAAAAT TCCGATTGAG 
GAAGTTTTCC AGCAGCTAAA ATGTTCAAGG GAAGGATTGA CAACGCAGGA AGGGGAGGAC 
AGGATTCAGA TCTTTGGCCC CAACAAGCTC GAAGAGAAAA AGGAAAGCAA ACTTCTGAAG 
TTTTTGGGGT TTATGTGGAA TCCACTTTCA TGGGTCATGG AAATGGCTGC AATCATGGCC 
ATTGCTTTGG CCAACGGTGA TGGTAGGCCT CCGGATTGGC AGGATTTTGT TGGTATTATC 
TGTCTGTTGG TTATCAACTC TACCATCAGT TTTATCGAAG AAAACAATGC TGGTAATGCT 
GCTGCTGCTC TTATGGCTGG TCTTGCTCCT AAAACCAAGG TTCTTAGGGA TGGAAAGTGG 
AGTGAACAAG AAGCTGCTAT TCTTGTCCCA GGAGATATTG TTAGCATTAA ATTAGGAGAC 
ATTATCCCAG CTGATGCCCG TCTACTTGAA GGTGATCCTT TAAAGGTTGA CCAATCTGCT 
CTAACTGGAG AGTCCCTTCC TGTAACCAAG CACCCGGGTC AAGAAGTTTT CTCTGGTTCA 
ACCTGCAAAC AAGGAGAAAT CGAGGCGGTT GTTATTGCCA CTGGGGTTCA TACCTTCTTC 
GGTAAAGCTG CTCACCTTGT GGACAGCACT AACCAAGTTG GACATTTCCA GAAGGTTCTT 
ACAGCCATTG GGAACTTCTG TATCTGTTCC ATTGCTATCG GTATGGTGAT TGAGATCATC 
GTCATGTATC CGATCCAACG CCGAAAGTAC AGAGATGGAA TTGACAACCT TTTGGTCCTC 
TTGATCGGTG GTATCCCCAT TGCTATGCCT ACAGTCTTGT CCGTGACCAT GGCTATTGGG 
TCTCACAGGT TGTCTCAGCA AGGTGCCATC ACCAAGCGTA TGACTGCCAT TGAAGAGATG 
GCAGGAATGG ATGTCCTGTG CAGTGACAAA ACCGGGACAC TAACCCTCAA CAAATTGAGT 
GTGGACAAAA ACTTGGTCGA GGTTTTCTGC AAGGGTGTGG AGAAAGATCA AGTCCTATTA 
TTTGCAGCTA TGGCTTCCAG GGTTGAGAAC CAGGATGCCA TTGATGCAGC CATGGTTGGG 
ATGCTTGCTG ATCCAAAGGA GGCTAGAGCT GGAATCAGGG AAGTTCACTT CCTTCCATTC 
AACCCTGTGG ATAAGAGAAC TGCTTTGACT TACATTGACG GCAGTGGTAA CTGGCACAGA 
GTCAGTAAAG GTGCTCCTGA GCAGATCCTC GAACTTGCCA AAGCCAGCAA TGATCTTAGC 
AAGAAGGTGC TCTCCATTAT TGACAAGTAT GCTGAGCGTG GTCTTAGGTC GTTGGCTGTT 
GCTCGCCAGG TGGTGCCAGA GAAAACAAAG GAAAGCCCAG GTGCGCCATG GGAATTTGTT 
GGCTTGTTGC CACTTTTTGA TCCCCCAAGA CATGACAGTG CTGAAACAAT TCGACGGGCT 
TTGAATCTTG GTGTTAACGT CAAGATGATC ACTGGTGACC AACTTGCTAT TGGTAAGGAA 
ACTGGTCGCA GACTTGGAAT GGGAACAAAC ATGTATCCAT CTTCGGCTCT TCTTGGTACA 
CACAAAGACG CAAACCTCGC ATCCATTCCT GTTGAGGAGT TGATTGAAAA GGCTGATGGA 
TTTGCCGGAG TCTTCCCAGG TTATAATCTG CTTATTTATT GTTTGGATTA TAAACCTCAC 
TATATGTTCA TTGCAAAGGT GGTGATGTTA GTTCTAAGCT TTGTTTTTTT TATTGCAGAG 
CACAAATACG AAATTGTGAA AAAGTTGCAG GAGAGGAAGC ATATTGTTGG AATGACTGGT 
GATGGTGTCA ATGATGCCCC TGCTCTAAAG AAAGCTGATA TCGGTATTGC TGTTGCTGAT 
GCTACAGATG CTGCTCGTGG TGCTTCAGAT ATCGTGCTCA CTGAGCCTGG ACTCAGCGTT 
ATTATCAGTG CTGTTCTCAC CAGCAGAGCT ATTTTCCAGA GAATGAAGAA CTATACTATC 
TATGCAGTCT CAATCACCAT CCGTATTGTG TTTGGTTTCA TGCTTATTGC TTTGATATGG 
GAATTTGACT TCTCAGCCTT CATGGTTCTG ATCATTGCCA TTCTTAACGA CGGTACCATC 
ATGACAATCT CAAAGGACAG AGTTAAGCCA TCTCCCACAC CTGATAGCTG GAAACTTAAA 
GAAATTTTTG CTACTGGAGT CGTTCTAGGA GGCTACCAGG CCATCATGAC TGTTATTTTC 
TTCTGGGCGG CGCACAAGAC TGACTTTTTC TCGGACACAT TCGGTGTGAG GTCCATTAGG 
GACAATAACC ACGAGCTAAT GGGTGCGGTG TACTTACAAG TTAGTATCAT TAGTCAAGCT 
CTGATCTTCG TCACAAGATC AAGGAGTTGG TCTTTTGTTG AACGTCCTGG AGCATTGCTG 
ATGATTGCTT TCCTCATTGC ACAACTGATT GCTACTTTGA TTGCGGTTTA CGCCAACTGG 
GAATTTGCAA AGATTAGGGG TATTGGATGG GGATGGGCTG GTGTGATCTG GCTATACAGT 
ATTGTCACAT ACTTCCCATT GGACGTTTTC AAGTTTGCCA TTCGATACAT CTTGAGCGGA 
AAGGCGTGGC TCAACTTGTT TGAGAACAAG ACGGCTTTCA CGATGAAGAA AGATTACGGA 
AAAGAAGAGA GAGAGGCTCA ATGGGCACTT GCTCAAAGGA CACTTCACGG TTTACAGCCA 
AAAGAAGCTG TTAACATCTT CCCTGAGAAA GGAAGTTACA GAGAATTGTC TGAGATCGCT 
GAGCAAGCTA AGAGAAGAGC TGAGATCGCT AGGCTTAGGG AGCTGCACAC ACTCAAGGGA 
CATGTGGAAT CAGTCGTGAA GCTAAAGGGC TTGGACATTG AAACTCCCAG TCACTACACT 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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HA2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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