EMB2770 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	EMB2770
Entrez Gene ID	827925
Full Name	pre-mRNA splicing factor-like protein
Synonyms	EMBRYO DEFECTIVE 2770,F9H3.5,F9H3_5,STA1,STABILIZED 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_116581.2	NP_192252.1	pre-mRNA splicing factor-like protein
NM_001340449.1	NP_001329764.1	pre-mRNA splicing factor-like protein
NM_001340448.1	NP_001319859.1	pre-mRNA splicing factor-like protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	PRPF6	NP_036601.2
Rattus norvegicus (Norway rat)	Prpf6	NP_001073234.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005640	XP_315659.1
Oryza sativa (rice)	Os10g0498600	NP_001064969.1
Xenopus tropicalis (tropical clawed frog)	prpf6	NP_001025642.1
Canis lupus familiaris (dog)	PRPF6	XP_534481.3
Mus musculus (house mouse)	Prpf6	NP_598462.1
Gallus gallus (chicken)	PRPF6	XP_417426.2
Magnaporthe oryzae (rice blast fungus)	MGG_07213	XP_003715426.1
Arabidopsis thaliana (thale cress)	EMB2770	NP_192252.1
Pan troglodytes (chimpanzee)	PRPF6	XP_001152053.3
Bos taurus (cattle)	PRPF6	NP_001039840.1
Danio rerio (zebrafish)	prpf6	NP_997820.1
Schizosaccharomyces pombe (fission yeast)	prp1	NP_596086.1
Caenorhabditis elegans (roundworm)	prp-6	NP_507525.1
Drosophila melanogaster (fruit fly)	CG6841	NP_649073.1
Neurospora crassa	NCU04606	XP_958849.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results suggest, splicing factor RDM16 and STA1 play an important role in RNA directed DNA Methylation pathway by affecting expression of key component genes including MORC6 by accumulation of exon 4, which facilitates key ATPase binding sites and MG2 + binding sites for DNA methylation.
Title: RDM16 and STA1 regulate differential usage of exon/intron in RNA directed DNA methylation pathway.

Findings indicate that heat-inducible STABILIZED1 (STA1) activity for pre-mRNA splicing serves as a molecular regulatory mechanism underlying the plant stress tolerance to high-temperature stress.
Title: STABILIZED1 Modulates Pre-mRNA Splicing for Thermotolerance.

The authors propose that STA1 acts downstream of siRNA biogenesis and facilitates the production of Pol V-dependent RNA transcripts in the RNA-directed DNA methylation pathway.
Title: The PRP6-like splicing factor STA1 is involved in RNA-directed DNA methylation by facilitating the production of Pol V-dependent scaffold RNAs.

The results suggest that STA1 is involved in miRNA biogenesis directly by functioning in pri-miRNA splicing and indirectly by modulating the DCL1 transcript level.
Title: STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis.

The following EMB2770 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EMB2770 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb15592	NM_001340449.1 Latest version!	Arabidopsis thaliana pre-mRNA splicing factor-like protein (EMB2770), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OAb15592	NM_116581.2 Latest version!	Arabidopsis thaliana pre-mRNA splicing factor-like protein (EMB2770), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OAb15592	NM_001340448.1 Latest version!	Arabidopsis thaliana pre-mRNA splicing factor-like protein (EMB2770), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EMB2770 ( NM_116581.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_192252.1 Arabidopsis thaliana pre-mRNA splicing factor-like protein (EMB2770), mRNA.

Price & Availability↑

CloneID	OAb15592
Clone ID Related Accession (Same CDS sequence)	NM_116581.2 , NM_001340449.1 , NM_001340448.1
Accession Version	NM_116581.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	pre-mRNA splicing factor-like protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_116581.1.

ATGGTGTTTC TCTCGATTCC AAACGGGAAG ACTTTATCGA TCGACGTGAA CCCTAATTCA 
ACCACCATCT CCGCCTTCGA ACAATTGGCC CACCAACGTA GTGATGTTCC ACAATCTTTC 
CTTCGTTACT CTCTCCGTAT GCGAAACCCT AGCCGCGTGT TTGTAGATTC AAAGGATTCC 
GATTCGATCT TATTGTCCGA TCTCGGTGTT TCTCGTTTCT CTACTGTGAT TATCCATGTC 
CTGCTTTTGG GTGGTATGCA AGCGGCTCCG CCGAAGCCTC GTCTTGATTT CCTCAATTCT 
AAGCCTCCGT CAAATTATGT AGCGGGTTTG GGTCGTGGTG CTACTGGGTT TACTACTCGA 
TCTGATATTG GTCCTGCTCG TGCTGCTCCG GATCTTCCTG ATCGGTCTGC TTTGGCTACA 
GCGGCTGCTC CAGGAGTAGG TCGTGGAGCA GGGAAGCCAA GTGAAGCGGA AGCGGAGGAC 
GATGAAGAAG CTGAGGAGAA AAGGTACGAT GAGAATCAGA CGTTTGATGA GTTTGAAGGG 
AATGATGTTG GTTTGTTCGC TAATGCTGAG TACGATGAGG ATGATAAAGA GGCTGATGCC 
ATCTGGGAGT CTATTGATCA GAGGATGGAT TCGAGGAGGA AAGATCGGAG AGAAGCGAAG 
CTGAAGGAAG AAATCGAGAA ATACCGAGCC TCGAACCCTA AGATTACTGA GCAGTTTGCG 
GATTTGAAGA GGAAATTACA CACTTTGTCT GCGGATGAGT GGGATAGTAT TCCTGAGATT 
GGGGATTACT CGCTGAGGAA CAAGAAGAAG AAGTTTGAGA GCTTTGTGCC TATTCCTGAT 
ACGCTTTTGG AGAAGGCGAA GAAAGAAAAG GAGCTTGTCA TGGCCTTAGA CCCAAAGAGC 
AGAGCCGCTG GTGGGTCGGA GACACCATGG GGGCAGACGC CGGTGACAGA CTTGACTGCT 
GTCGGTGAGG GAAGAGGTAC GGTGTTGTCT CTGAAGCTTG ATAACCTATC AGATTCAGTT 
TCGGGGCAAA CTGTAGTGGA TCCTAAAGGC TACTTAACTG ACCTGAAGAG TATGAAAAGA 
ACCACTGATG AAGAGATATA CGATCGCAAT AGAGCTAGAT TGTTATACAA GAGTCTGACC 
CAGTCGAATC CAAAGAATCC AAATGGCTGG ATTGCTGCTG CTAGAGTGGA GGAGGTGGAT 
GGGAAGATTA AAGCAGCTAG GTTTCAGATT CAGAGGGGCT GCGAAGAGTG CCCGAAAAAT 
GAGGATGTTT GGCTTGAAGC TTGTAGGTTA GCTAATCCAG AAGATGCCAA GGGGGTTATT 
GCGAAGGGAG TTAAGCTGAT ACCCAATTCA GTGAAGCTAT GGTTGGAGGC TGCAAAGCTG 
GAGCATGATG TGGAGAACAA GAGTAGGGTG TTGAGAAAGG GACTGGAACA TATTCCAGAC 
TCTGTAAGAC TATGGAAGGC TGTTGTTGAG TTGGCTAATG AGGAGGATGC AAGGATTTTG 
CTTCACAGGG CTGTGGAGTG CTGCCCTTTG CATCTGGAGC TATGGGTGGC ACTTGCGAGG 
CTCGAAACAT ATGCTGAATC AAAGAAGGTG TTGAACAAAG CGAGAGAGAA GCTCCCAAAG 
GAGCCTGCAA TTTGGATCAC CGCTGCTAAG TTAGAAGAAG CTAATGGGAA GCTAGATGAA 
GCTAATGATA ACACGGCTAT GGTGGGAAAG ATTATTGATA GGGGTATAAA GACTCTCCAG 
AGAGAAGGGG TTGTTATTGA CCGAGAAAAT TGGATGAGTG AGGCTGAGGC ATGTGAGAGA 
GTTGGATCTG TCGCTACATG CCAGGCCATT ATTAAGAACA CAATAGGTAT TGGAGTGGAA 
GAAGAGGATA GAAAGAGAAC TTGGGTTGCT GATGCAGATG AGTGCAAGAA GAGGGGTTCC 
ATTGAGACCG CAAGAGCAAT ATACGCCCAT GCTCTTAGTG TATTCTTGAC CAAGAAAAGT 
ATCTGGCTTA AAGCGGCGCA GCTTGAGAAG AGCCATGGGA GTCGGGAGTC CCTTGATGCC 
TTGTTGCGTA AGGCTGTAAC ATATGTCCCG CAAGCTGAGG TTCTCTGGCT CATGGGTGCC 
AAAGAGAAGT GGCTCGCTGG AGATGTTCCA GCAGCCCGTG CTATTCTACA AGAGGCTTAT 
GCTGCAATTC CCAACTCTGA GGAAATCTGG CTTGCTGCTT TCAAGCTTGA GTTTGAGAAC 
AAGGAGCCGG AGAGGGCAAG AATGCTGCTT GCAAAAGCAA GGGAAAGAGG AGGGACTGAA 
AGGGTCTGGA TGAAATCAGC CATTGTTGAG AGAGAACTTG GCAACGTAGA GGAGGAGAGG 
AGATTGCTTA ATGAAGGTTT GAAGCAATTC CCAACATTCT TCAAGCTTTG GTTAATGCTT 
GGGCAGCTCG AGGAACGGTT CAAGCATCTA GAACAGGCCA GGAAAGCTTA TGACACTGGT 
TTGAAGCACT GTCCCCACTG CATACCACTG TGGCTCTCAC TCGCTGATCT TGAAGAGAAA 
GTGAATGGGC TAAACAAAGC TCGGGCTATT CTCACCACGG CCAGGAAGAA GAATCCTGGG 
GGGGCTGAGC TATGGTTAGC TGCTATTCGC GCTGAACTGA GGCATGACAA CAAGAGAGAA 
GCAGAGCACT TGATGTCAAA GGCTCTGCAA GACTGTCCCA AGAGTGGTAT CCTCTGGGCT 
GCGGACATTG AGATGGCGCC ACGTCCTCGA CGGAAAACAA AGAGTATTGA TGCTATGAAG 
AAGTGTGATC GCGACCCTCA TGTCACCATA GCTGTTGCCA AGCTCTTTTG GCAAGACAAG 
AAGGTGGAGA AAGCCAGAGC ATGGTTTGAA CGTGCTGTTA CCGTTGGCCC AGATATTGGC 
GATTTCTGGG CGTTGTTCTA CAAATTTGAA CTTCAACATG GCTCTGATGA GGACAGGAAG 
GAAGTTGTGG CGAAGTGTGT AGCTTGTGAG CCGAAGCATG GCGAGAAATG GCAAGCCATC 
TCCAAAGCGG TTGAAAATGC TCACCAGCCT ATTGAAGTTA TCTTGAAGAG AGTTGTGAAT 
GCGTTGAGCA AGGAAGAGAA TTCTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_192252.1
CDS	64..3153
Translation	MVFLSIPNGKTLSIDVNPNSTTISAFEQLAHQRSDVPQSFLRYSLRMRNPSRVFVDSKDSDSILLSDLGVSRFSTVIIHVLLLGGMQAAPPKPRLDFLNSKPPSNYVAGLGRGATGFTTRSDIGPARAAPDLPDRSALATAAAPGVGRGAGKPSEAEAEDDEEAEEKRYDENQTFDEFEGNDVGLFANAEYDEDDKEADAIWESIDQRMDSRRKDRREAKLKEEIEKYRASNPKITEQFADLKRKLHTLSADEWDSIPEIGDYSLRNKKKKFESFVPIPDTLLEKAKKEKELVMALDPKSRAAGGSETPWGQTPVTDLTAVGEGRGTVLSLKLDNLSDSVSGQTVVDPKGYLTDLKSMKRTTDEEIYDRNRARLLYKSLTQSNPKNPNGWIAAARVEEVDGKIKAARFQIQRGCEECPKNEDVWLEACRLANPEDAKGVIAKGVKLIPNSVKLWLEAAKLEHDVENKSRVLRKGLEHIPDSVRLWKAVVELANEEDARILLHRAVECCPLHLELWVALARLETYAESKKVLNKAREKLPKEPAIWITAAKLEEANGKLDEANDNTAMVGKIIDRGIKTLQREGVVIDRENWMSEAEACERVGSVATCQAIIKNTIGIGVEEEDRKRTWVADADECKKRGSIETARAIYAHALSVFLTKKSIWLKAAQLEKSHGSRESLDALLRKAVTYVPQAEVLWLMGAKEKWLAGDVPAARAILQEAYAAIPNSEEIWLAAFKLEFENKEPERARMLLAKARERGGTERVWMKSAIVERELGNVEEERRLLNEGLKQFPTFFKLWLMLGQLEERFKHLEQARKAYDTGLKHCPHCIPLWLSLADLEEKVNGLNKARAILTTARKKNPGGAELWLAAIRAELRHDNKREAEHLMSKALQDCPKSGILWAADIEMAPRPRRKTKSIDAMKKCDRDPHVTIAVAKLFWQDKKVEKARAWFERAVTVGPDIGDFWALFYKFELQHGSDEDRKEVVAKCVACEPKHGEKWQAISKAVENAHQPIEVILKRVVNALSKEENSA

Target ORF information:

RefSeq Version	NM_116581.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana pre-mRNA splicing factor-like protein (EMB2770), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_116581.2

ORF Insert Sequence:

ATGGTGTTTC TCTCGATTCC AAACGGGAAG ACTTTATCGA TCGACGTGAA CCCTAATTCA 
ACCACCATCT CCGCCTTCGA ACAATTGGCC CACCAACGTA GTGATGTTCC ACAATCTTTC 
CTTCGTTACT CTCTCCGTAT GCGAAACCCT AGCCGCGTGT TTGTAGATTC AAAGGATTCC 
GATTCGATCT TATTGTCCGA TCTCGGTGTT TCTCGTTTCT CTACTGTGAT TATCCATGTC 
CTGCTTTTGG GTGGTATGCA AGCGGCTCCG CCGAAGCCTC GTCTTGATTT CCTCAATTCT 
AAGCCTCCGT CAAATTATGT AGCGGGTTTG GGTCGTGGTG CTACTGGGTT TACTACTCGA 
TCTGATATTG GTCCTGCTCG TGCTGCTCCG GATCTTCCTG ATCGGTCTGC TTTGGCTACA 
GCGGCTGCTC CAGGAGTAGG TCGTGGAGCA GGGAAGCCAA GTGAAGCGGA AGCGGAGGAC 
GATGAAGAAG CTGAGGAGAA AAGGTACGAT GAGAATCAGA CGTTTGATGA GTTTGAAGGG 
AATGATGTTG GTTTGTTCGC TAATGCTGAG TACGATGAGG ATGATAAAGA GGCTGATGCC 
ATCTGGGAGT CTATTGATCA GAGGATGGAT TCGAGGAGGA AAGATCGGAG AGAAGCGAAG 
CTGAAGGAAG AAATCGAGAA ATACCGAGCC TCGAACCCTA AGATTACTGA GCAGTTTGCG 
GATTTGAAGA GGAAATTACA CACTTTGTCT GCGGATGAGT GGGATAGTAT TCCTGAGATT 
GGGGATTACT CGCTGAGGAA CAAGAAGAAG AAGTTTGAGA GCTTTGTGCC TATTCCTGAT 
ACGCTTTTGG AGAAGGCGAA GAAAGAAAAG GAGCTTGTCA TGGCCTTAGA CCCAAAGAGC 
AGAGCCGCTG GTGGGTCGGA GACACCATGG GGGCAGACGC CGGTGACAGA CTTGACTGCT 
GTCGGTGAGG GAAGAGGTAC GGTGTTGTCT CTGAAGCTTG ATAACCTATC AGATTCAGTT 
TCGGGGCAAA CTGTAGTGGA TCCTAAAGGC TACTTAACTG ACCTGAAGAG TATGAAAAGA 
ACCACTGATG AAGAGATATA CGATCGCAAT AGAGCTAGAT TGTTATACAA GAGTCTGACC 
CAGTCGAATC CAAAGAATCC AAATGGCTGG ATTGCTGCTG CTAGAGTGGA GGAGGTGGAT 
GGGAAGATTA AAGCAGCTAG GTTTCAGATT CAGAGGGGCT GCGAAGAGTG CCCGAAAAAT 
GAGGATGTTT GGCTTGAAGC TTGTAGGTTA GCTAATCCAG AAGATGCCAA GGGGGTTATT 
GCGAAGGGAG TTAAGCTGAT ACCCAATTCA GTGAAGCTAT GGTTGGAGGC TGCAAAGCTG 
GAGCATGATG TGGAGAACAA GAGTAGGGTG TTGAGAAAGG GACTGGAACA TATTCCAGAC 
TCTGTAAGAC TATGGAAGGC TGTTGTTGAG TTGGCTAATG AGGAGGATGC AAGGATTTTG 
CTTCACAGGG CTGTGGAGTG CTGCCCTTTG CATCTGGAGC TATGGGTGGC ACTTGCGAGG 
CTCGAAACAT ATGCTGAATC AAAGAAGGTG TTGAACAAAG CGAGAGAGAA GCTCCCAAAG 
GAGCCTGCAA TTTGGATCAC CGCTGCTAAG TTAGAAGAAG CTAATGGGAA GCTAGATGAA 
GCTAATGATA ACACGGCTAT GGTGGGAAAG ATTATTGATA GGGGTATAAA GACTCTCCAG 
AGAGAAGGGG TTGTTATTGA CCGAGAAAAT TGGATGAGTG AGGCTGAGGC ATGTGAGAGA 
GTTGGATCTG TCGCTACATG CCAGGCCATT ATTAAGAACA CAATAGGTAT TGGAGTGGAA 
GAAGAGGATA GAAAGAGAAC TTGGGTTGCT GATGCAGATG AGTGCAAGAA GAGGGGTTCC 
ATTGAGACCG CAAGAGCAAT ATACGCCCAT GCTCTTAGTG TATTCTTGAC CAAGAAAAGT 
ATCTGGCTTA AAGCGGCGCA GCTTGAGAAG AGCCATGGGA GTCGGGAGTC CCTTGATGCC 
TTGTTGCGTA AGGCTGTAAC ATATGTCCCG CAAGCTGAGG TTCTCTGGCT CATGGGTGCC 
AAAGAGAAGT GGCTCGCTGG AGATGTTCCA GCAGCCCGTG CTATTCTACA AGAGGCTTAT 
GCTGCAATTC CCAACTCTGA GGAAATCTGG CTTGCTGCTT TCAAGCTTGA GTTTGAGAAC 
AAGGAGCCGG AGAGGGCAAG AATGCTGCTT GCAAAAGCAA GGGAAAGAGG AGGGACTGAA 
AGGGTCTGGA TGAAATCAGC CATTGTTGAG AGAGAACTTG GCAACGTAGA GGAGGAGAGG 
AGATTGCTTA ATGAAGGTTT GAAGCAATTC CCAACATTCT TCAAGCTTTG GTTAATGCTT 
GGGCAGCTCG AGGAACGGTT CAAGCATCTA GAACAGGCCA GGAAAGCTTA TGACACTGGT 
TTGAAGCACT GTCCCCACTG CATACCACTG TGGCTCTCAC TCGCTGATCT TGAAGAGAAA 
GTGAATGGGC TAAACAAAGC TCGGGCTATT CTCACCACGG CCAGGAAGAA GAATCCTGGG 
GGGGCTGAGC TATGGTTAGC TGCTATTCGC GCTGAACTGA GGCATGACAA CAAGAGAGAA 
GCAGAGCACT TGATGTCAAA GGCTCTGCAA GACTGTCCCA AGAGTGGTAT CCTCTGGGCT 
GCGGACATTG AGATGGCGCC ACGTCCTCGA CGGAAAACAA AGAGTATTGA TGCTATGAAG 
AAGTGTGATC GCGACCCTCA TGTCACCATA GCTGTTGCCA AGCTCTTTTG GCAAGACAAG 
AAGGTGGAGA AAGCCAGAGC ATGGTTTGAA CGTGCTGTTA CCGTTGGCCC AGATATTGGC 
GATTTCTGGG CGTTGTTCTA CAAATTTGAA CTTCAACATG GCTCTGATGA GGACAGGAAG 
GAAGTTGTGG CGAAGTGTGT AGCTTGTGAG CCGAAGCATG GCGAGAAATG GCAAGCCATC 
TCCAAAGCGG TTGAAAATGC TCACCAGCCT ATTGAAGTTA TCTTGAAGAG AGTTGTGAAT 
GCGTTGAGCA AGGAAGAGAA TTCTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMB2770 ( NM_001340449.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001329764.1 Arabidopsis thaliana pre-mRNA splicing factor-like protein (EMB2770), mRNA.

Price & Availability↑

CloneID	OAb15592
Clone ID Related Accession (Same CDS sequence)	NM_116581.2 , NM_001340449.1 , NM_001340448.1
Accession Version	NM_001340449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	pre-mRNA splicing factor-like protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGGTGTTTC TCTCGATTCC AAACGGGAAG ACTTTATCGA TCGACGTGAA CCCTAATTCA 
ACCACCATCT CCGCCTTCGA ACAATTGGCC CACCAACGTA GTGATGTTCC ACAATCTTTC 
CTTCGTTACT CTCTCCGTAT GCGAAACCCT AGCCGCGTGT TTGTAGATTC AAAGGATTCC 
GATTCGATCT TATTGTCCGA TCTCGGTGTT TCTCGTTTCT CTACTGTGAT TATCCATGTC 
CTGCTTTTGG GTGGTATGCA AGCGGCTCCG CCGAAGCCTC GTCTTGATTT CCTCAATTCT 
AAGCCTCCGT CAAATTATGT AGCGGGTTTG GGTCGTGGTG CTACTGGGTT TACTACTCGA 
TCTGATATTG GTCCTGCTCG TGCTGCTCCG GATCTTCCTG ATCGGTCTGC TTTGGCTACA 
GCGGCTGCTC CAGGAGTAGG TCGTGGAGCA GGGAAGCCAA GTGAAGCGGA AGCGGAGGAC 
GATGAAGAAG CTGAGGAGAA AAGGTACGAT GAGAATCAGA CGTTTGATGA GTTTGAAGGG 
AATGATGTTG GTTTGTTCGC TAATGCTGAG TACGATGAGG ATGATAAAGA GGCTGATGCC 
ATCTGGGAGT CTATTGATCA GAGGATGGAT TCGAGGAGGA AAGATCGGAG AGAAGCGAAG 
CTGAAGGAAG AAATCGAGAA ATACCGAGCC TCGAACCCTA AGATTACTGA GCAGTTTGCG 
GATTTGAAGA GGAAATTACA CACTTTGTCT GCGGATGAGT GGGATAGTAT TCCTGAGATT 
GGGGATTACT CGCTGAGGAA CAAGAAGAAG AAGTTTGAGA GCTTTGTGCC TATTCCTGAT 
ACGCTTTTGG AGAAGGCGAA GAAAGAAAAG GAGCTTGTCA TGGCCTTAGA CCCAAAGAGC 
AGAGCCGCTG GTGGGTCGGA GACACCATGG GGGCAGACGC CGGTGACAGA CTTGACTGCT 
GTCGGTGAGG GAAGAGGTAC GGTGTTGTCT CTGAAGCTTG ATAACCTATC AGATTCAGTT 
TCGGGGCAAA CTGTAGTGGA TCCTAAAGGC TACTTAACTG ACCTGAAGAG TATGAAAAGA 
ACCACTGATG AAGAGATATA CGATCGCAAT AGAGCTAGAT TGTTATACAA GAGTCTGACC 
CAGTCGAATC CAAAGAATCC AAATGGCTGG ATTGCTGCTG CTAGAGTGGA GGAGGTGGAT 
GGGAAGATTA AAGCAGCTAG GTTTCAGATT CAGAGGGGCT GCGAAGAGTG CCCGAAAAAT 
GAGGATGTTT GGCTTGAAGC TTGTAGGTTA GCTAATCCAG AAGATGCCAA GGGGGTTATT 
GCGAAGGGAG TTAAGCTGAT ACCCAATTCA GTGAAGCTAT GGTTGGAGGC TGCAAAGCTG 
GAGCATGATG TGGAGAACAA GAGTAGGGTG TTGAGAAAGG GACTGGAACA TATTCCAGAC 
TCTGTAAGAC TATGGAAGGC TGTTGTTGAG TTGGCTAATG AGGAGGATGC AAGGATTTTG 
CTTCACAGGG CTGTGGAGTG CTGCCCTTTG CATCTGGAGC TATGGGTGGC ACTTGCGAGG 
CTCGAAACAT ATGCTGAATC AAAGAAGGTG TTGAACAAAG CGAGAGAGAA GCTCCCAAAG 
GAGCCTGCAA TTTGGATCAC CGCTGCTAAG TTAGAAGAAG CTAATGGGAA GCTAGATGAA 
GCTAATGATA ACACGGCTAT GGTGGGAAAG ATTATTGATA GGGGTATAAA GACTCTCCAG 
AGAGAAGGGG TTGTTATTGA CCGAGAAAAT TGGATGAGTG AGGCTGAGGC ATGTGAGAGA 
GTTGGATCTG TCGCTACATG CCAGGCCATT ATTAAGAACA CAATAGGTAT TGGAGTGGAA 
GAAGAGGATA GAAAGAGAAC TTGGGTTGCT GATGCAGATG AGTGCAAGAA GAGGGGTTCC 
ATTGAGACCG CAAGAGCAAT ATACGCCCAT GCTCTTAGTG TATTCTTGAC CAAGAAAAGT 
ATCTGGCTTA AAGCGGCGCA GCTTGAGAAG AGCCATGGGA GTCGGGAGTC CCTTGATGCC 
TTGTTGCGTA AGGCTGTAAC ATATGTCCCG CAAGCTGAGG TTCTCTGGCT CATGGGTGCC 
AAAGAGAAGT GGCTCGCTGG AGATGTTCCA GCAGCCCGTG CTATTCTACA AGAGGCTTAT 
GCTGCAATTC CCAACTCTGA GGAAATCTGG CTTGCTGCTT TCAAGCTTGA GTTTGAGAAC 
AAGGAGCCGG AGAGGGCAAG AATGCTGCTT GCAAAAGCAA GGGAAAGAGG AGGGACTGAA 
AGGGTCTGGA TGAAATCAGC CATTGTTGAG AGAGAACTTG GCAACGTAGA GGAGGAGAGG 
AGATTGCTTA ATGAAGGTTT GAAGCAATTC CCAACATTCT TCAAGCTTTG GTTAATGCTT 
GGGCAGCTCG AGGAACGGTT CAAGCATCTA GAACAGGCCA GGAAAGCTTA TGACACTGGT 
TTGAAGCACT GTCCCCACTG CATACCACTG TGGCTCTCAC TCGCTGATCT TGAAGAGAAA 
GTGAATGGGC TAAACAAAGC TCGGGCTATT CTCACCACGG CCAGGAAGAA GAATCCTGGG 
GGGGCTGAGC TATGGTTAGC TGCTATTCGC GCTGAACTGA GGCATGACAA CAAGAGAGAA 
GCAGAGCACT TGATGTCAAA GGCTCTGCAA GACTGTCCCA AGAGTGGTAT CCTCTGGGCT 
GCGGACATTG AGATGGCGCC ACGTCCTCGA CGGAAAACAA AGAGTATTGA TGCTATGAAG 
AAGTGTGATC GCGACCCTCA TGTCACCATA GCTGTTGCCA AGCTCTTTTG GCAAGACAAG 
AAGGTGGAGA AAGCCAGAGC ATGGTTTGAA CGTGCTGTTA CCGTTGGCCC AGATATTGGC 
GATTTCTGGG CGTTGTTCTA CAAATTTGAA CTTCAACATG GCTCTGATGA GGACAGGAAG 
GAAGTTGTGG CGAAGTGTGT AGCTTGTGAG CCGAAGCATG GCGAGAAATG GCAAGCCATC 
TCCAAAGCGG TTGAAAATGC TCACCAGCCT ATTGAAGTTA TCTTGAAGAG AGTTGTGAAT 
GCGTTGAGCA AGGAAGAGAA TTCTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001329764.1
CDS	64..3153
Translation	MVFLSIPNGKTLSIDVNPNSTTISAFEQLAHQRSDVPQSFLRYSLRMRNPSRVFVDSKDSDSILLSDLGVSRFSTVIIHVLLLGGMQAAPPKPRLDFLNSKPPSNYVAGLGRGATGFTTRSDIGPARAAPDLPDRSALATAAAPGVGRGAGKPSEAEAEDDEEAEEKRYDENQTFDEFEGNDVGLFANAEYDEDDKEADAIWESIDQRMDSRRKDRREAKLKEEIEKYRASNPKITEQFADLKRKLHTLSADEWDSIPEIGDYSLRNKKKKFESFVPIPDTLLEKAKKEKELVMALDPKSRAAGGSETPWGQTPVTDLTAVGEGRGTVLSLKLDNLSDSVSGQTVVDPKGYLTDLKSMKRTTDEEIYDRNRARLLYKSLTQSNPKNPNGWIAAARVEEVDGKIKAARFQIQRGCEECPKNEDVWLEACRLANPEDAKGVIAKGVKLIPNSVKLWLEAAKLEHDVENKSRVLRKGLEHIPDSVRLWKAVVELANEEDARILLHRAVECCPLHLELWVALARLETYAESKKVLNKAREKLPKEPAIWITAAKLEEANGKLDEANDNTAMVGKIIDRGIKTLQREGVVIDRENWMSEAEACERVGSVATCQAIIKNTIGIGVEEEDRKRTWVADADECKKRGSIETARAIYAHALSVFLTKKSIWLKAAQLEKSHGSRESLDALLRKAVTYVPQAEVLWLMGAKEKWLAGDVPAARAILQEAYAAIPNSEEIWLAAFKLEFENKEPERARMLLAKARERGGTERVWMKSAIVERELGNVEEERRLLNEGLKQFPTFFKLWLMLGQLEERFKHLEQARKAYDTGLKHCPHCIPLWLSLADLEEKVNGLNKARAILTTARKKNPGGAELWLAAIRAELRHDNKREAEHLMSKALQDCPKSGILWAADIEMAPRPRRKTKSIDAMKKCDRDPHVTIAVAKLFWQDKKVEKARAWFERAVTVGPDIGDFWALFYKFELQHGSDEDRKEVVAKCVACEPKHGEKWQAISKAVENAHQPIEVILKRVVNALSKEENSA

Target ORF information:

RefSeq Version	NM_001340449.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana pre-mRNA splicing factor-like protein (EMB2770), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001340449.1

ORF Insert Sequence:

ATGGTGTTTC TCTCGATTCC AAACGGGAAG ACTTTATCGA TCGACGTGAA CCCTAATTCA 
ACCACCATCT CCGCCTTCGA ACAATTGGCC CACCAACGTA GTGATGTTCC ACAATCTTTC 
CTTCGTTACT CTCTCCGTAT GCGAAACCCT AGCCGCGTGT TTGTAGATTC AAAGGATTCC 
GATTCGATCT TATTGTCCGA TCTCGGTGTT TCTCGTTTCT CTACTGTGAT TATCCATGTC 
CTGCTTTTGG GTGGTATGCA AGCGGCTCCG CCGAAGCCTC GTCTTGATTT CCTCAATTCT 
AAGCCTCCGT CAAATTATGT AGCGGGTTTG GGTCGTGGTG CTACTGGGTT TACTACTCGA 
TCTGATATTG GTCCTGCTCG TGCTGCTCCG GATCTTCCTG ATCGGTCTGC TTTGGCTACA 
GCGGCTGCTC CAGGAGTAGG TCGTGGAGCA GGGAAGCCAA GTGAAGCGGA AGCGGAGGAC 
GATGAAGAAG CTGAGGAGAA AAGGTACGAT GAGAATCAGA CGTTTGATGA GTTTGAAGGG 
AATGATGTTG GTTTGTTCGC TAATGCTGAG TACGATGAGG ATGATAAAGA GGCTGATGCC 
ATCTGGGAGT CTATTGATCA GAGGATGGAT TCGAGGAGGA AAGATCGGAG AGAAGCGAAG 
CTGAAGGAAG AAATCGAGAA ATACCGAGCC TCGAACCCTA AGATTACTGA GCAGTTTGCG 
GATTTGAAGA GGAAATTACA CACTTTGTCT GCGGATGAGT GGGATAGTAT TCCTGAGATT 
GGGGATTACT CGCTGAGGAA CAAGAAGAAG AAGTTTGAGA GCTTTGTGCC TATTCCTGAT 
ACGCTTTTGG AGAAGGCGAA GAAAGAAAAG GAGCTTGTCA TGGCCTTAGA CCCAAAGAGC 
AGAGCCGCTG GTGGGTCGGA GACACCATGG GGGCAGACGC CGGTGACAGA CTTGACTGCT 
GTCGGTGAGG GAAGAGGTAC GGTGTTGTCT CTGAAGCTTG ATAACCTATC AGATTCAGTT 
TCGGGGCAAA CTGTAGTGGA TCCTAAAGGC TACTTAACTG ACCTGAAGAG TATGAAAAGA 
ACCACTGATG AAGAGATATA CGATCGCAAT AGAGCTAGAT TGTTATACAA GAGTCTGACC 
CAGTCGAATC CAAAGAATCC AAATGGCTGG ATTGCTGCTG CTAGAGTGGA GGAGGTGGAT 
GGGAAGATTA AAGCAGCTAG GTTTCAGATT CAGAGGGGCT GCGAAGAGTG CCCGAAAAAT 
GAGGATGTTT GGCTTGAAGC TTGTAGGTTA GCTAATCCAG AAGATGCCAA GGGGGTTATT 
GCGAAGGGAG TTAAGCTGAT ACCCAATTCA GTGAAGCTAT GGTTGGAGGC TGCAAAGCTG 
GAGCATGATG TGGAGAACAA GAGTAGGGTG TTGAGAAAGG GACTGGAACA TATTCCAGAC 
TCTGTAAGAC TATGGAAGGC TGTTGTTGAG TTGGCTAATG AGGAGGATGC AAGGATTTTG 
CTTCACAGGG CTGTGGAGTG CTGCCCTTTG CATCTGGAGC TATGGGTGGC ACTTGCGAGG 
CTCGAAACAT ATGCTGAATC AAAGAAGGTG TTGAACAAAG CGAGAGAGAA GCTCCCAAAG 
GAGCCTGCAA TTTGGATCAC CGCTGCTAAG TTAGAAGAAG CTAATGGGAA GCTAGATGAA 
GCTAATGATA ACACGGCTAT GGTGGGAAAG ATTATTGATA GGGGTATAAA GACTCTCCAG 
AGAGAAGGGG TTGTTATTGA CCGAGAAAAT TGGATGAGTG AGGCTGAGGC ATGTGAGAGA 
GTTGGATCTG TCGCTACATG CCAGGCCATT ATTAAGAACA CAATAGGTAT TGGAGTGGAA 
GAAGAGGATA GAAAGAGAAC TTGGGTTGCT GATGCAGATG AGTGCAAGAA GAGGGGTTCC 
ATTGAGACCG CAAGAGCAAT ATACGCCCAT GCTCTTAGTG TATTCTTGAC CAAGAAAAGT 
ATCTGGCTTA AAGCGGCGCA GCTTGAGAAG AGCCATGGGA GTCGGGAGTC CCTTGATGCC 
TTGTTGCGTA AGGCTGTAAC ATATGTCCCG CAAGCTGAGG TTCTCTGGCT CATGGGTGCC 
AAAGAGAAGT GGCTCGCTGG AGATGTTCCA GCAGCCCGTG CTATTCTACA AGAGGCTTAT 
GCTGCAATTC CCAACTCTGA GGAAATCTGG CTTGCTGCTT TCAAGCTTGA GTTTGAGAAC 
AAGGAGCCGG AGAGGGCAAG AATGCTGCTT GCAAAAGCAA GGGAAAGAGG AGGGACTGAA 
AGGGTCTGGA TGAAATCAGC CATTGTTGAG AGAGAACTTG GCAACGTAGA GGAGGAGAGG 
AGATTGCTTA ATGAAGGTTT GAAGCAATTC CCAACATTCT TCAAGCTTTG GTTAATGCTT 
GGGCAGCTCG AGGAACGGTT CAAGCATCTA GAACAGGCCA GGAAAGCTTA TGACACTGGT 
TTGAAGCACT GTCCCCACTG CATACCACTG TGGCTCTCAC TCGCTGATCT TGAAGAGAAA 
GTGAATGGGC TAAACAAAGC TCGGGCTATT CTCACCACGG CCAGGAAGAA GAATCCTGGG 
GGGGCTGAGC TATGGTTAGC TGCTATTCGC GCTGAACTGA GGCATGACAA CAAGAGAGAA 
GCAGAGCACT TGATGTCAAA GGCTCTGCAA GACTGTCCCA AGAGTGGTAT CCTCTGGGCT 
GCGGACATTG AGATGGCGCC ACGTCCTCGA CGGAAAACAA AGAGTATTGA TGCTATGAAG 
AAGTGTGATC GCGACCCTCA TGTCACCATA GCTGTTGCCA AGCTCTTTTG GCAAGACAAG 
AAGGTGGAGA AAGCCAGAGC ATGGTTTGAA CGTGCTGTTA CCGTTGGCCC AGATATTGGC 
GATTTCTGGG CGTTGTTCTA CAAATTTGAA CTTCAACATG GCTCTGATGA GGACAGGAAG 
GAAGTTGTGG CGAAGTGTGT AGCTTGTGAG CCGAAGCATG GCGAGAAATG GCAAGCCATC 
TCCAAAGCGG TTGAAAATGC TCACCAGCCT ATTGAAGTTA TCTTGAAGAG AGTTGTGAAT 
GCGTTGAGCA AGGAAGAGAA TTCTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMB2770 ( NM_001340448.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001319859.1 Arabidopsis thaliana pre-mRNA splicing factor-like protein (EMB2770), mRNA.

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CloneID	OAb15592
Clone ID Related Accession (Same CDS sequence)	NM_116581.2 , NM_001340449.1 , NM_001340448.1
Accession Version	NM_001340448.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	pre-mRNA splicing factor-like protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGGTGTTTC TCTCGATTCC AAACGGGAAG ACTTTATCGA TCGACGTGAA CCCTAATTCA 
ACCACCATCT CCGCCTTCGA ACAATTGGCC CACCAACGTA GTGATGTTCC ACAATCTTTC 
CTTCGTTACT CTCTCCGTAT GCGAAACCCT AGCCGCGTGT TTGTAGATTC AAAGGATTCC 
GATTCGATCT TATTGTCCGA TCTCGGTGTT TCTCGTTTCT CTACTGTGAT TATCCATGTC 
CTGCTTTTGG GTGGTATGCA AGCGGCTCCG CCGAAGCCTC GTCTTGATTT CCTCAATTCT 
AAGCCTCCGT CAAATTATGT AGCGGGTTTG GGTCGTGGTG CTACTGGGTT TACTACTCGA 
TCTGATATTG GTCCTGCTCG TGCTGCTCCG GATCTTCCTG ATCGGTCTGC TTTGGCTACA 
GCGGCTGCTC CAGGAGTAGG TCGTGGAGCA GGGAAGCCAA GTGAAGCGGA AGCGGAGGAC 
GATGAAGAAG CTGAGGAGAA AAGGTACGAT GAGAATCAGA CGTTTGATGA GTTTGAAGGG 
AATGATGTTG GTTTGTTCGC TAATGCTGAG TACGATGAGG ATGATAAAGA GGCTGATGCC 
ATCTGGGAGT CTATTGATCA GAGGATGGAT TCGAGGAGGA AAGATCGGAG AGAAGCGAAG 
CTGAAGGAAG AAATCGAGAA ATACCGAGCC TCGAACCCTA AGATTACTGA GCAGTTTGCG 
GATTTGAAGA GGAAATTACA CACTTTGTCT GCGGATGAGT GGGATAGTAT TCCTGAGATT 
GGGGATTACT CGCTGAGGAA CAAGAAGAAG AAGTTTGAGA GCTTTGTGCC TATTCCTGAT 
ACGCTTTTGG AGAAGGCGAA GAAAGAAAAG GAGCTTGTCA TGGCCTTAGA CCCAAAGAGC 
AGAGCCGCTG GTGGGTCGGA GACACCATGG GGGCAGACGC CGGTGACAGA CTTGACTGCT 
GTCGGTGAGG GAAGAGGTAC GGTGTTGTCT CTGAAGCTTG ATAACCTATC AGATTCAGTT 
TCGGGGCAAA CTGTAGTGGA TCCTAAAGGC TACTTAACTG ACCTGAAGAG TATGAAAAGA 
ACCACTGATG AAGAGATATA CGATCGCAAT AGAGCTAGAT TGTTATACAA GAGTCTGACC 
CAGTCGAATC CAAAGAATCC AAATGGCTGG ATTGCTGCTG CTAGAGTGGA GGAGGTGGAT 
GGGAAGATTA AAGCAGCTAG GTTTCAGATT CAGAGGGGCT GCGAAGAGTG CCCGAAAAAT 
GAGGATGTTT GGCTTGAAGC TTGTAGGTTA GCTAATCCAG AAGATGCCAA GGGGGTTATT 
GCGAAGGGAG TTAAGCTGAT ACCCAATTCA GTGAAGCTAT GGTTGGAGGC TGCAAAGCTG 
GAGCATGATG TGGAGAACAA GAGTAGGGTG TTGAGAAAGG GACTGGAACA TATTCCAGAC 
TCTGTAAGAC TATGGAAGGC TGTTGTTGAG TTGGCTAATG AGGAGGATGC AAGGATTTTG 
CTTCACAGGG CTGTGGAGTG CTGCCCTTTG CATCTGGAGC TATGGGTGGC ACTTGCGAGG 
CTCGAAACAT ATGCTGAATC AAAGAAGGTG TTGAACAAAG CGAGAGAGAA GCTCCCAAAG 
GAGCCTGCAA TTTGGATCAC CGCTGCTAAG TTAGAAGAAG CTAATGGGAA GCTAGATGAA 
GCTAATGATA ACACGGCTAT GGTGGGAAAG ATTATTGATA GGGGTATAAA GACTCTCCAG 
AGAGAAGGGG TTGTTATTGA CCGAGAAAAT TGGATGAGTG AGGCTGAGGC ATGTGAGAGA 
GTTGGATCTG TCGCTACATG CCAGGCCATT ATTAAGAACA CAATAGGTAT TGGAGTGGAA 
GAAGAGGATA GAAAGAGAAC TTGGGTTGCT GATGCAGATG AGTGCAAGAA GAGGGGTTCC 
ATTGAGACCG CAAGAGCAAT ATACGCCCAT GCTCTTAGTG TATTCTTGAC CAAGAAAAGT 
ATCTGGCTTA AAGCGGCGCA GCTTGAGAAG AGCCATGGGA GTCGGGAGTC CCTTGATGCC 
TTGTTGCGTA AGGCTGTAAC ATATGTCCCG CAAGCTGAGG TTCTCTGGCT CATGGGTGCC 
AAAGAGAAGT GGCTCGCTGG AGATGTTCCA GCAGCCCGTG CTATTCTACA AGAGGCTTAT 
GCTGCAATTC CCAACTCTGA GGAAATCTGG CTTGCTGCTT TCAAGCTTGA GTTTGAGAAC 
AAGGAGCCGG AGAGGGCAAG AATGCTGCTT GCAAAAGCAA GGGAAAGAGG AGGGACTGAA 
AGGGTCTGGA TGAAATCAGC CATTGTTGAG AGAGAACTTG GCAACGTAGA GGAGGAGAGG 
AGATTGCTTA ATGAAGGTTT GAAGCAATTC CCAACATTCT TCAAGCTTTG GTTAATGCTT 
GGGCAGCTCG AGGAACGGTT CAAGCATCTA GAACAGGCCA GGAAAGCTTA TGACACTGGT 
TTGAAGCACT GTCCCCACTG CATACCACTG TGGCTCTCAC TCGCTGATCT TGAAGAGAAA 
GTGAATGGGC TAAACAAAGC TCGGGCTATT CTCACCACGG CCAGGAAGAA GAATCCTGGG 
GGGGCTGAGC TATGGTTAGC TGCTATTCGC GCTGAACTGA GGCATGACAA CAAGAGAGAA 
GCAGAGCACT TGATGTCAAA GGCTCTGCAA GACTGTCCCA AGAGTGGTAT CCTCTGGGCT 
GCGGACATTG AGATGGCGCC ACGTCCTCGA CGGAAAACAA AGAGTATTGA TGCTATGAAG 
AAGTGTGATC GCGACCCTCA TGTCACCATA GCTGTTGCCA AGCTCTTTTG GCAAGACAAG 
AAGGTGGAGA AAGCCAGAGC ATGGTTTGAA CGTGCTGTTA CCGTTGGCCC AGATATTGGC 
GATTTCTGGG CGTTGTTCTA CAAATTTGAA CTTCAACATG GCTCTGATGA GGACAGGAAG 
GAAGTTGTGG CGAAGTGTGT AGCTTGTGAG CCGAAGCATG GCGAGAAATG GCAAGCCATC 
TCCAAAGCGG TTGAAAATGC TCACCAGCCT ATTGAAGTTA TCTTGAAGAG AGTTGTGAAT 
GCGTTGAGCA AGGAAGAGAA TTCTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001319859.1
CDS	64..3153
Translation	MVFLSIPNGKTLSIDVNPNSTTISAFEQLAHQRSDVPQSFLRYSLRMRNPSRVFVDSKDSDSILLSDLGVSRFSTVIIHVLLLGGMQAAPPKPRLDFLNSKPPSNYVAGLGRGATGFTTRSDIGPARAAPDLPDRSALATAAAPGVGRGAGKPSEAEAEDDEEAEEKRYDENQTFDEFEGNDVGLFANAEYDEDDKEADAIWESIDQRMDSRRKDRREAKLKEEIEKYRASNPKITEQFADLKRKLHTLSADEWDSIPEIGDYSLRNKKKKFESFVPIPDTLLEKAKKEKELVMALDPKSRAAGGSETPWGQTPVTDLTAVGEGRGTVLSLKLDNLSDSVSGQTVVDPKGYLTDLKSMKRTTDEEIYDRNRARLLYKSLTQSNPKNPNGWIAAARVEEVDGKIKAARFQIQRGCEECPKNEDVWLEACRLANPEDAKGVIAKGVKLIPNSVKLWLEAAKLEHDVENKSRVLRKGLEHIPDSVRLWKAVVELANEEDARILLHRAVECCPLHLELWVALARLETYAESKKVLNKAREKLPKEPAIWITAAKLEEANGKLDEANDNTAMVGKIIDRGIKTLQREGVVIDRENWMSEAEACERVGSVATCQAIIKNTIGIGVEEEDRKRTWVADADECKKRGSIETARAIYAHALSVFLTKKSIWLKAAQLEKSHGSRESLDALLRKAVTYVPQAEVLWLMGAKEKWLAGDVPAARAILQEAYAAIPNSEEIWLAAFKLEFENKEPERARMLLAKARERGGTERVWMKSAIVERELGNVEEERRLLNEGLKQFPTFFKLWLMLGQLEERFKHLEQARKAYDTGLKHCPHCIPLWLSLADLEEKVNGLNKARAILTTARKKNPGGAELWLAAIRAELRHDNKREAEHLMSKALQDCPKSGILWAADIEMAPRPRRKTKSIDAMKKCDRDPHVTIAVAKLFWQDKKVEKARAWFERAVTVGPDIGDFWALFYKFELQHGSDEDRKEVVAKCVACEPKHGEKWQAISKAVENAHQPIEVILKRVVNALSKEENSA

Target ORF information:

RefSeq Version	NM_001340448.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana pre-mRNA splicing factor-like protein (EMB2770), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001340448.1

ORF Insert Sequence:

ATGGTGTTTC TCTCGATTCC AAACGGGAAG ACTTTATCGA TCGACGTGAA CCCTAATTCA 
ACCACCATCT CCGCCTTCGA ACAATTGGCC CACCAACGTA GTGATGTTCC ACAATCTTTC 
CTTCGTTACT CTCTCCGTAT GCGAAACCCT AGCCGCGTGT TTGTAGATTC AAAGGATTCC 
GATTCGATCT TATTGTCCGA TCTCGGTGTT TCTCGTTTCT CTACTGTGAT TATCCATGTC 
CTGCTTTTGG GTGGTATGCA AGCGGCTCCG CCGAAGCCTC GTCTTGATTT CCTCAATTCT 
AAGCCTCCGT CAAATTATGT AGCGGGTTTG GGTCGTGGTG CTACTGGGTT TACTACTCGA 
TCTGATATTG GTCCTGCTCG TGCTGCTCCG GATCTTCCTG ATCGGTCTGC TTTGGCTACA 
GCGGCTGCTC CAGGAGTAGG TCGTGGAGCA GGGAAGCCAA GTGAAGCGGA AGCGGAGGAC 
GATGAAGAAG CTGAGGAGAA AAGGTACGAT GAGAATCAGA CGTTTGATGA GTTTGAAGGG 
AATGATGTTG GTTTGTTCGC TAATGCTGAG TACGATGAGG ATGATAAAGA GGCTGATGCC 
ATCTGGGAGT CTATTGATCA GAGGATGGAT TCGAGGAGGA AAGATCGGAG AGAAGCGAAG 
CTGAAGGAAG AAATCGAGAA ATACCGAGCC TCGAACCCTA AGATTACTGA GCAGTTTGCG 
GATTTGAAGA GGAAATTACA CACTTTGTCT GCGGATGAGT GGGATAGTAT TCCTGAGATT 
GGGGATTACT CGCTGAGGAA CAAGAAGAAG AAGTTTGAGA GCTTTGTGCC TATTCCTGAT 
ACGCTTTTGG AGAAGGCGAA GAAAGAAAAG GAGCTTGTCA TGGCCTTAGA CCCAAAGAGC 
AGAGCCGCTG GTGGGTCGGA GACACCATGG GGGCAGACGC CGGTGACAGA CTTGACTGCT 
GTCGGTGAGG GAAGAGGTAC GGTGTTGTCT CTGAAGCTTG ATAACCTATC AGATTCAGTT 
TCGGGGCAAA CTGTAGTGGA TCCTAAAGGC TACTTAACTG ACCTGAAGAG TATGAAAAGA 
ACCACTGATG AAGAGATATA CGATCGCAAT AGAGCTAGAT TGTTATACAA GAGTCTGACC 
CAGTCGAATC CAAAGAATCC AAATGGCTGG ATTGCTGCTG CTAGAGTGGA GGAGGTGGAT 
GGGAAGATTA AAGCAGCTAG GTTTCAGATT CAGAGGGGCT GCGAAGAGTG CCCGAAAAAT 
GAGGATGTTT GGCTTGAAGC TTGTAGGTTA GCTAATCCAG AAGATGCCAA GGGGGTTATT 
GCGAAGGGAG TTAAGCTGAT ACCCAATTCA GTGAAGCTAT GGTTGGAGGC TGCAAAGCTG 
GAGCATGATG TGGAGAACAA GAGTAGGGTG TTGAGAAAGG GACTGGAACA TATTCCAGAC 
TCTGTAAGAC TATGGAAGGC TGTTGTTGAG TTGGCTAATG AGGAGGATGC AAGGATTTTG 
CTTCACAGGG CTGTGGAGTG CTGCCCTTTG CATCTGGAGC TATGGGTGGC ACTTGCGAGG 
CTCGAAACAT ATGCTGAATC AAAGAAGGTG TTGAACAAAG CGAGAGAGAA GCTCCCAAAG 
GAGCCTGCAA TTTGGATCAC CGCTGCTAAG TTAGAAGAAG CTAATGGGAA GCTAGATGAA 
GCTAATGATA ACACGGCTAT GGTGGGAAAG ATTATTGATA GGGGTATAAA GACTCTCCAG 
AGAGAAGGGG TTGTTATTGA CCGAGAAAAT TGGATGAGTG AGGCTGAGGC ATGTGAGAGA 
GTTGGATCTG TCGCTACATG CCAGGCCATT ATTAAGAACA CAATAGGTAT TGGAGTGGAA 
GAAGAGGATA GAAAGAGAAC TTGGGTTGCT GATGCAGATG AGTGCAAGAA GAGGGGTTCC 
ATTGAGACCG CAAGAGCAAT ATACGCCCAT GCTCTTAGTG TATTCTTGAC CAAGAAAAGT 
ATCTGGCTTA AAGCGGCGCA GCTTGAGAAG AGCCATGGGA GTCGGGAGTC CCTTGATGCC 
TTGTTGCGTA AGGCTGTAAC ATATGTCCCG CAAGCTGAGG TTCTCTGGCT CATGGGTGCC 
AAAGAGAAGT GGCTCGCTGG AGATGTTCCA GCAGCCCGTG CTATTCTACA AGAGGCTTAT 
GCTGCAATTC CCAACTCTGA GGAAATCTGG CTTGCTGCTT TCAAGCTTGA GTTTGAGAAC 
AAGGAGCCGG AGAGGGCAAG AATGCTGCTT GCAAAAGCAA GGGAAAGAGG AGGGACTGAA 
AGGGTCTGGA TGAAATCAGC CATTGTTGAG AGAGAACTTG GCAACGTAGA GGAGGAGAGG 
AGATTGCTTA ATGAAGGTTT GAAGCAATTC CCAACATTCT TCAAGCTTTG GTTAATGCTT 
GGGCAGCTCG AGGAACGGTT CAAGCATCTA GAACAGGCCA GGAAAGCTTA TGACACTGGT 
TTGAAGCACT GTCCCCACTG CATACCACTG TGGCTCTCAC TCGCTGATCT TGAAGAGAAA 
GTGAATGGGC TAAACAAAGC TCGGGCTATT CTCACCACGG CCAGGAAGAA GAATCCTGGG 
GGGGCTGAGC TATGGTTAGC TGCTATTCGC GCTGAACTGA GGCATGACAA CAAGAGAGAA 
GCAGAGCACT TGATGTCAAA GGCTCTGCAA GACTGTCCCA AGAGTGGTAT CCTCTGGGCT 
GCGGACATTG AGATGGCGCC ACGTCCTCGA CGGAAAACAA AGAGTATTGA TGCTATGAAG 
AAGTGTGATC GCGACCCTCA TGTCACCATA GCTGTTGCCA AGCTCTTTTG GCAAGACAAG 
AAGGTGGAGA AAGCCAGAGC ATGGTTTGAA CGTGCTGTTA CCGTTGGCCC AGATATTGGC 
GATTTCTGGG CGTTGTTCTA CAAATTTGAA CTTCAACATG GCTCTGATGA GGACAGGAAG 
GAAGTTGTGG CGAAGTGTGT AGCTTGTGAG CCGAAGCATG GCGAGAAATG GCAAGCCATC 
TCCAAAGCGG TTGAAAATGC TCACCAGCCT ATTGAAGTTA TCTTGAAGAG AGTTGTGAAT 
GCGTTGAGCA AGGAAGAGAA TTCTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
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EMB2770 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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