MSH4 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	MSH4
Entrez Gene ID	827450
Full Name	MUTS-like protein 4
Synonyms	ARABIDOPSIS MUTS HOMOLOG 4,ARABIDOPSIS MUTS HOMOLOG 4,ATMSH4,DL4725W,FCAALL.423,MUTS HOMOLOG 4,MUTS HOMOLOG 4,MUTS-like protein 4
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001341219.1	NP_001327948.1	MUTS-like protein 4
NM_117842.3	NP_193469.2	MUTS-like protein 4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MSH4	XP_524742.2
Kluyveromyces lactis	KLLA0F11022g	XP_455582.1
Canis lupus familiaris (dog)	MSH4	XP_005622187.1
Danio rerio (zebrafish)	msh4	XP_688406.1
Caenorhabditis elegans (roundworm)	him-14	NP_495451.1
Magnaporthe oryzae (rice blast fungus)	MGG_08738	XP_003719247.1
Arabidopsis thaliana (thale cress)	MSH4	NP_193469.2
Homo sapiens (human)	MSH4	NP_002431.2
	MSH4	XP_001101368.1
Gallus gallus (chicken)	MSH4	XP_004936961.1
Mus musculus (house mouse)	Msh4	NP_114076.1
Rattus norvegicus (Norway rat)	Msh4	NP_001099947.2
Saccharomyces cerevisiae (baker's yeast)	MSH4	NP_116652.1
Xenopus tropicalis (tropical clawed frog)	msh4	XP_002936134.2
Bos taurus (cattle)	MSH4	NP_001193184.1
Oryza sativa (rice)	Os07g0486000	NP_001175201.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Localization of AtMSH5 to the chromatin at mid-prophase I is dependent on expression of AtMSH4.
Title: AtMSH5 partners AtMSH4 in the class I meiotic crossover pathway in Arabidopsis thaliana, but is not required for synapsis.

The following MSH4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MSH4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb15195	NM_117842.3 Latest version!	Arabidopsis thaliana MUTS-like protein 4 (MSH4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb39418	NM_001341219.1 Latest version!	Arabidopsis thaliana MUTS-like protein 4 (MSH4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MSH4 ( NM_117842.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_193469.2 Arabidopsis thaliana MUTS-like protein 4 (MSH4), mRNA.

Price & Availability↑

CloneID	OAb15195
Clone ID Related Accession (Same CDS sequence)	NM_117842.3
Accession Version	NM_117842.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	MUTS-like protein 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Apr 18, 2007 this sequence version replaced NM_117842.2.

ATGGAAGACG ACGGAGGAGA GAGATCGAGT TTCGTCGCCG GCCTAATTGA GAACAGAGCC 
AAAGAGGTTG GAATGGCTGC TTTTGACCTA AGATCGGCTT CACTGCATCT ATCTCAGTAT 
ATAGAGACAA GCAGCTCATA CCAAAACACG AAGACGCTGT TGCGTTTCTA TGATCCTTCT 
GTGATAATTG TTCCTCCCAA CAAATTGGCA GCCGATGGCA TGGTTGGGGT ATCGGAACTG 
GTGGATAGAT GTTATAGCAC CGTTAGGAAG GTTGTGTTTG CCCGTGGCTG TTTTGATGAC 
ACCAAGGGTG CTGTGCTAAT ACAAAACTTG GCAGCAGAAG AACCTTTGGC GCTTGGTTTA 
GACACTTACT ATAAGCAGCA TTATCTCTCA TTGGCTGCTG CTGCTGCTAC AATCAAATGG 
ATAGAGGCGG AAAAGGGTGT GATAGTCACC AACCACTCAC TGACTGTAAC TTTTAATGGA 
TCGTTTGATC ACATGAATAT CGATGCAACT AGTGTTGAGA ATCTGGAACT CATTGATCCA 
TTCCACAACG CACTTTTGGG CACTAGCAAC AAAAAGAGGA GTTTGTTCCA GATGTTCAAG 
ACAACAAAAA CAGCTGGCGG GACTAGGCTT CTTCGCGCAA ACTTGTTACA GCCTCTGAAA 
GATATTGAAA CTATTAACAC TCGCCTGGAC TGCCTGGATG AGTTGATGAG CAATGAACAA 
CTATTCTTTG GGCTTTCACA AGTTTTACGA AAATTTCCAA AAGAGACTGA TCGAGTTCTT 
TGTCATTTCT GCTTCAAGCC AAAGAAAGTC ACTGAAGCGG TCATAGGTTT TGAAAATACC 
AGAAAGAGCC AAAACATGAT TTCAAGCATT ATCTTACTTA AGACTGCATT GGATGCTTTA 
CCTATACTTG CAAAGGTTCT GAAGGACGCA AAGTGTTTTC TTCTTGCCAA TGTTTACAAG 
TCAGTGTGTG AAAATGATAG ATATGCTTCC ATCAGAAAAA AAATCGGGGA AGTTATTGAT 
GATGATGTCC TCCATGCACG GGTTCCGTTT GTTGCCCGTA CACAGCAGTG TTTTGCTTTA 
AAAGCCGGAA TTGATGGATT TTTGGATATT GCAAGAAGAA CCTTCTGTGA TACTAGTGAA 
GCAATACATA ATCTGGCCAG TAAGTATCGT GAGGAATTCA ACTTGCCAAA TTTGAAACTC 
CCTTTCAACA ACAGGCAGGG ATTCTTTTTC AGAATTCCTC AGAAGGAAGT TCAGGGAAAA 
CTTCCGAACA AATTCACTCA GGTTGTAAAA CATGGGAAAA ACATACATTG TTCGAGTCTG 
GAACTGGCTT CTTTGAATGT CAGGAATAAG TCTGCCGCTG GAGAGTGTTT CATTCGAACG 
GAAACATGCT TGGAAGCACT AATGGATGCT ATAAGGGAAG ATATCTCTGC CCTCACACTC 
CTAGCAGAAG TCTTATGTCT CCTAGATATG ATTGTTAATT CATTTGCTCA TACAATATCC 
ACAAAGCCAG TGGATCGATA CTCTAGACCT GAACTAACAG ATAGTGGCCC ACTGGCAATT 
GATGCTGGAA GGCATCCCAT CCTAGAAAGC ATACACAACG ACTTTGTTTC TAATAGTATA 
TTCATGTCAG AAGCAACCAA CATGTTGGTA GTTATGGGTC CAAACATGAG CGGAAAGAGC 
ACATATCTCC AACAGGTGTG TCTAGTAGTC ATTCTTGCTC AGATTGGTTG CTATGTCCCA 
GCTCGCTTTG CAACTATAAG GGTGGTTGAC CGCATATTTA CAAGAATGGG GACAATGGAT 
AACCTTGAGT CGAATTCCAG CACGTTTATG ACGGAGATGA GAGAGACCGC TTTCATAATG 
CAGAATGTCA CCAATAGGAG TCTGATAGTC ATGGATGAAC TCGGGAGGGC TACTTCTTCC 
TCAGACGGGT TAGCAATGGC ATGGAGCTGC TGCGAGTATC TCTTATCACT CAAAGCGTAC 
ACAGTATTTG CAACCCATAT GGACAGTCTC GCAGAGTTAG CAACCATCTA CCCAAATGTC 
AAGGTTCTTC ATTTTTATGT AGACATCAGA GATAACCGCT TAGACTTCAA GTTTCAACTG 
CGAGATGGAA CCCTACATGT TCCACATTAT GGCCTTCTAT TAGCTGAAGT GGCGGGTCTG 
CCTAGTACAG TGATCGATAC AGCTAGGATC ATAACCAAAA GGATCACAGA CAAAGAAAAT 
AAAAGAATAG AGCTAAATTG TGGGAAGCAC CACGAAATAC ATCGGATCTA CCGAGTTGCT 
CAAAGGTTGA TATGCTTGAA ATACTCTAGA CAGACAGAAG ACTCAATTCG TCAAGCACTC 
CAAAATCTGA ATGAAAGCTT CACTGAAGAA AGACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_193469.2
CDS	59..2437
Translation	MEDDGGERSSFVAGLIENRAKEVGMAAFDLRSASLHLSQYIETSSSYQNTKTLLRFYDPSVIIVPPNKLAADGMVGVSELVDRCYSTVRKVVFARGCFDDTKGAVLIQNLAAEEPLALGLDTYYKQHYLSLAAAAATIKWIEAEKGVIVTNHSLTVTFNGSFDHMNIDATSVENLELIDPFHNALLGTSNKKRSLFQMFKTTKTAGGTRLLRANLLQPLKDIETINTRLDCLDELMSNEQLFFGLSQVLRKFPKETDRVLCHFCFKPKKVTEAVIGFENTRKSQNMISSIILLKTALDALPILAKVLKDAKCFLLANVYKSVCENDRYASIRKKIGEVIDDDVLHARVPFVARTQQCFALKAGIDGFLDIARRTFCDTSEAIHNLASKYREEFNLPNLKLPFNNRQGFFFRIPQKEVQGKLPNKFTQVVKHGKNIHCSSLELASLNVRNKSAAGECFIRTETCLEALMDAIREDISALTLLAEVLCLLDMIVNSFAHTISTKPVDRYSRPELTDSGPLAIDAGRHPILESIHNDFVSNSIFMSEATNMLVVMGPNMSGKSTYLQQVCLVVILAQIGCYVPARFATIRVVDRIFTRMGTMDNLESNSSTFMTEMRETAFIMQNVTNRSLIVMDELGRATSSSDGLAMAWSCCEYLLSLKAYTVFATHMDSLAELATIYPNVKVLHFYVDIRDNRLDFKFQLRDGTLHVPHYGLLLAEVAGLPSTVIDTARIITKRITDKENKRIELNCGKHHEIHRIYRVAQRLICLKYSRQTEDSIRQALQNLNESFTEERL

Target ORF information:

RefSeq Version	NM_117842.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana MUTS-like protein 4 (MSH4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_117842.3

ORF Insert Sequence:

ATGGAAGACG ACGGAGGAGA GAGATCGAGT TTCGTCGCCG GCCTAATTGA GAACAGAGCC 
AAAGAGGTTG GAATGGCTGC TTTTGACCTA AGATCGGCTT CACTGCATCT ATCTCAGTAT 
ATAGAGACAA GCAGCTCATA CCAAAACACG AAGACGCTGT TGCGTTTCTA TGATCCTTCT 
GTGATAATTG TTCCTCCCAA CAAATTGGCA GCCGATGGCA TGGTTGGGGT ATCGGAACTG 
GTGGATAGAT GTTATAGCAC CGTTAGGAAG GTTGTGTTTG CCCGTGGCTG TTTTGATGAC 
ACCAAGGGTG CTGTGCTAAT ACAAAACTTG GCAGCAGAAG AACCTTTGGC GCTTGGTTTA 
GACACTTACT ATAAGCAGCA TTATCTCTCA TTGGCTGCTG CTGCTGCTAC AATCAAATGG 
ATAGAGGCGG AAAAGGGTGT GATAGTCACC AACCACTCAC TGACTGTAAC TTTTAATGGA 
TCGTTTGATC ACATGAATAT CGATGCAACT AGTGTTGAGA ATCTGGAACT CATTGATCCA 
TTCCACAACG CACTTTTGGG CACTAGCAAC AAAAAGAGGA GTTTGTTCCA GATGTTCAAG 
ACAACAAAAA CAGCTGGCGG GACTAGGCTT CTTCGCGCAA ACTTGTTACA GCCTCTGAAA 
GATATTGAAA CTATTAACAC TCGCCTGGAC TGCCTGGATG AGTTGATGAG CAATGAACAA 
CTATTCTTTG GGCTTTCACA AGTTTTACGA AAATTTCCAA AAGAGACTGA TCGAGTTCTT 
TGTCATTTCT GCTTCAAGCC AAAGAAAGTC ACTGAAGCGG TCATAGGTTT TGAAAATACC 
AGAAAGAGCC AAAACATGAT TTCAAGCATT ATCTTACTTA AGACTGCATT GGATGCTTTA 
CCTATACTTG CAAAGGTTCT GAAGGACGCA AAGTGTTTTC TTCTTGCCAA TGTTTACAAG 
TCAGTGTGTG AAAATGATAG ATATGCTTCC ATCAGAAAAA AAATCGGGGA AGTTATTGAT 
GATGATGTCC TCCATGCACG GGTTCCGTTT GTTGCCCGTA CACAGCAGTG TTTTGCTTTA 
AAAGCCGGAA TTGATGGATT TTTGGATATT GCAAGAAGAA CCTTCTGTGA TACTAGTGAA 
GCAATACATA ATCTGGCCAG TAAGTATCGT GAGGAATTCA ACTTGCCAAA TTTGAAACTC 
CCTTTCAACA ACAGGCAGGG ATTCTTTTTC AGAATTCCTC AGAAGGAAGT TCAGGGAAAA 
CTTCCGAACA AATTCACTCA GGTTGTAAAA CATGGGAAAA ACATACATTG TTCGAGTCTG 
GAACTGGCTT CTTTGAATGT CAGGAATAAG TCTGCCGCTG GAGAGTGTTT CATTCGAACG 
GAAACATGCT TGGAAGCACT AATGGATGCT ATAAGGGAAG ATATCTCTGC CCTCACACTC 
CTAGCAGAAG TCTTATGTCT CCTAGATATG ATTGTTAATT CATTTGCTCA TACAATATCC 
ACAAAGCCAG TGGATCGATA CTCTAGACCT GAACTAACAG ATAGTGGCCC ACTGGCAATT 
GATGCTGGAA GGCATCCCAT CCTAGAAAGC ATACACAACG ACTTTGTTTC TAATAGTATA 
TTCATGTCAG AAGCAACCAA CATGTTGGTA GTTATGGGTC CAAACATGAG CGGAAAGAGC 
ACATATCTCC AACAGGTGTG TCTAGTAGTC ATTCTTGCTC AGATTGGTTG CTATGTCCCA 
GCTCGCTTTG CAACTATAAG GGTGGTTGAC CGCATATTTA CAAGAATGGG GACAATGGAT 
AACCTTGAGT CGAATTCCAG CACGTTTATG ACGGAGATGA GAGAGACCGC TTTCATAATG 
CAGAATGTCA CCAATAGGAG TCTGATAGTC ATGGATGAAC TCGGGAGGGC TACTTCTTCC 
TCAGACGGGT TAGCAATGGC ATGGAGCTGC TGCGAGTATC TCTTATCACT CAAAGCGTAC 
ACAGTATTTG CAACCCATAT GGACAGTCTC GCAGAGTTAG CAACCATCTA CCCAAATGTC 
AAGGTTCTTC ATTTTTATGT AGACATCAGA GATAACCGCT TAGACTTCAA GTTTCAACTG 
CGAGATGGAA CCCTACATGT TCCACATTAT GGCCTTCTAT TAGCTGAAGT GGCGGGTCTG 
CCTAGTACAG TGATCGATAC AGCTAGGATC ATAACCAAAA GGATCACAGA CAAAGAAAAT 
AAAAGAATAG AGCTAAATTG TGGGAAGCAC CACGAAATAC ATCGGATCTA CCGAGTTGCT 
CAAAGGTTGA TATGCTTGAA ATACTCTAGA CAGACAGAAG ACTCAATTCG TCAAGCACTC 
CAAAATCTGA ATGAAAGCTT CACTGAAGAA AGACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MSH4 ( NM_001341219.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001327948.1 Arabidopsis thaliana MUTS-like protein 4 (MSH4), partial mRNA.

Price & Availability↑

CloneID	OAb39418
Clone ID Related Accession (Same CDS sequence)	NM_001341219.1
Accession Version	NM_001341219.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	MUTS-like protein 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). COMPLETENESS: incomplete on the 3' end.

ATGGAAGACG ACGGAGGAGA GAGATCGAGT TTCGTCGCCG GCCTAATTGA GAACAGAGCC 
AAAGAGGTTG GAATGGCTGC TTTTGACCTA AGATCGGCTT CACTGCATCT ATCTCAGTAT 
ATAGAGACAA GCAGCTCATA CCAAAACACG AAGACGCTGT TGCGTTTCTA TGATCCTTCT 
GTGATAATTG TTCCTCCCAA CAAATTGGCA GCCGATGGCA TGGTTGGGGT ATCGGAACTG 
GTGGATAGAT GTTATAGCAC CGTTAGGAAG GTTGTGTTTG CCCGTGGCTG TTTTGATGAC 
ACCAAGGGTG CTGTGCTAAT ACAAAACTTG GCAGCAGAAG AACCTTTGGC GCTTGGTTTA 
GACACTTACT ATAAGCAGCA TTATCTCTCA TTGGCTGCTG CTGCTGCTAC AATCAAATGG 
ATAGAGGCGG AAAAGGGTGT GATAGTCACC AACCACTCAC TGACTGTAAC TTTTAATGGA 
TCGTTTGATC ACATGAATAT CGATGCAACT AGTGTTGAGA ATCTGGAACT CATTGATCCA 
TTCCACAACG CACTTTTGGG CACTAGCAAC AAAAAGAGGA GTTTGTTCCA GATGTTCAAG 
ACAACAAAAA CAGCTGGCGG GACTAGGCTT CTTCGCGCAA ACTTGTTACA GCCTCTGAAA 
GATATTGAAA CTATTAACAC TCGCCTGGAC TGCCTGGATG AGTTGATGAG CAATGAACAA 
CTATTCTTTG GGCTTTCACA AGTTTTACGA AAATTTCCAA AAGAGACTGA TCGAGTTCTT 
TGTCATTTCT GCTTCAAGCC AAAGAAAGTC ACTGAAGCGG TCATAGGTTT TGAAAATACC 
AGAAAGAGCC AAAACATGAT TTCAAGCATT ATCTTACTTA AGACTGCATT GGATGCTTTA 
CCTATACTTG CAAAGGTTCT GAAGGACGCA AAGTGTTTTC TTCTTGCCAA TGTTTACAAG 
TCAGTGTGTG AAAATGATAG ATATGCTTCC ATCAGAAAAA AAATCGGGGA AGTTATTGAT 
GATGATGTCC TCCATGCACG GGTTCCGTTT GTTGCCCGTA CACAGCAGTG TTTTGCTTTA 
AAAGCCGGAA TTGATGGATT TTTGGATATT GCAAGAAGAA CCTTCTGTGA TACTAGTGAA 
GCAATACATA ATCTGGCCAG TAAGTATCGT GAGGAATTCA ACTTGCCAAA TTTGAAACTC 
CCTTTCAACA ACAGGCAGGG ATTCTTTTTC AGAATTCCTC AGAAGGAAGT TCAGGGAAAA 
CTTCCGAACA AATTCACTCA GGTTGTAAAA CATGGGAAAA ACATACATTG TTCGAGTCTG 
GAACTGGCTT CTTTGAATGT CAGGAATAAG TCTGCCGCTG GAGAGTGTTT CATTCGAACG 
GAAACATGCT TGGAAGCACT AATGGATGCT ATAAGGGAAG ATATCTCTGC CCTCACACTC 
CTAGCAGAAG TCTTATGTCT CCTAGATATG ATTGTTAATT CATTTGCTCA TACAATATCC 
ACAAAGCCAG TGGATCGATA CTCTAGACCT GAACTAACAG ATAGTGGCCC ACTGGCAATT 
GATGCTGGAA GGCATCCCAT CCTAGAAAGC ATACACAACG ACTTTGTTTC TAATAGTATA 
TTCATGTCAG AAGCAACCAA CATGTTGGTA GTTATGGGTC CAAACATGAG CGGAAAGAGC 
ACATATCTCC AACAGGTGTG TCTAGTAGTC ATTCTTGCTC AGATTGGTTG CTATGTCCCA 
GCTCGCTTTG CAACTATAAG GGTGGTTGAC CGCATATTTA CAAGAATGGG GACAATGGAT 
AACCTTGAGT CGAATTCCAG CACGTTTATG ACGGAGATGA GAGAGACCGC TTTCATAATG 
CAGAATGTCA CCAATAGGAG TCTGATAGTC ATGGATGAAC TCGGGAGGGC TACTTCTTCC 
TCAGACGGGT TAGCAATGGC ATGGAGCTGC TGCGAGTATC TCTTATCACT CAAAGCGTAC 
ACAGTATTTG CAACCCATAT GGACAGTCTC GCAGAGTTAG CAACCATCTA CCCAAATGTC 
AAGGTTCTTC ATTTTTATGT AGACATCAGA GATAACCGCT TAGACTTCAA GGTTCTGTCT 
GTCTTCCTTT CGTATGAGTT CGAGACTTTA GGTACTGCTG CTGAATGTTA CATTGTTCTG 
ATCAGTTTCA ACTGCGAGAT GGAACCCTAC ATGTTCCACA TTATGGCCTT CTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001327948.1
CDS	59..2275
Translation	MEDDGGERSSFVAGLIENRAKEVGMAAFDLRSASLHLSQYIETSSSYQNTKTLLRFYDPSVIIVPPNKLAADGMVGVSELVDRCYSTVRKVVFARGCFDDTKGAVLIQNLAAEEPLALGLDTYYKQHYLSLAAAAATIKWIEAEKGVIVTNHSLTVTFNGSFDHMNIDATSVENLELIDPFHNALLGTSNKKRSLFQMFKTTKTAGGTRLLRANLLQPLKDIETINTRLDCLDELMSNEQLFFGLSQVLRKFPKETDRVLCHFCFKPKKVTEAVIGFENTRKSQNMISSIILLKTALDALPILAKVLKDAKCFLLANVYKSVCENDRYASIRKKIGEVIDDDVLHARVPFVARTQQCFALKAGIDGFLDIARRTFCDTSEAIHNLASKYREEFNLPNLKLPFNNRQGFFFRIPQKEVQGKLPNKFTQVVKHGKNIHCSSLELASLNVRNKSAAGECFIRTETCLEALMDAIREDISALTLLAEVLCLLDMIVNSFAHTISTKPVDRYSRPELTDSGPLAIDAGRHPILESIHNDFVSNSIFMSEATNMLVVMGPNMSGKSTYLQQVCLVVILAQIGCYVPARFATIRVVDRIFTRMGTMDNLESNSSTFMTEMRETAFIMQNVTNRSLIVMDELGRATSSSDGLAMAWSCCEYLLSLKAYTVFATHMDSLAELATIYPNVKVLHFYVDIRDNRLDFKVLSVFLSYEFETLGTAAECYIVLISFNCEMEPYMFHIMAFY

Target ORF information:

RefSeq Version	NM_001341219.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana MUTS-like protein 4 (MSH4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001341219.1

ORF Insert Sequence:

ATGGAAGACG ACGGAGGAGA GAGATCGAGT TTCGTCGCCG GCCTAATTGA GAACAGAGCC 
AAAGAGGTTG GAATGGCTGC TTTTGACCTA AGATCGGCTT CACTGCATCT ATCTCAGTAT 
ATAGAGACAA GCAGCTCATA CCAAAACACG AAGACGCTGT TGCGTTTCTA TGATCCTTCT 
GTGATAATTG TTCCTCCCAA CAAATTGGCA GCCGATGGCA TGGTTGGGGT ATCGGAACTG 
GTGGATAGAT GTTATAGCAC CGTTAGGAAG GTTGTGTTTG CCCGTGGCTG TTTTGATGAC 
ACCAAGGGTG CTGTGCTAAT ACAAAACTTG GCAGCAGAAG AACCTTTGGC GCTTGGTTTA 
GACACTTACT ATAAGCAGCA TTATCTCTCA TTGGCTGCTG CTGCTGCTAC AATCAAATGG 
ATAGAGGCGG AAAAGGGTGT GATAGTCACC AACCACTCAC TGACTGTAAC TTTTAATGGA 
TCGTTTGATC ACATGAATAT CGATGCAACT AGTGTTGAGA ATCTGGAACT CATTGATCCA 
TTCCACAACG CACTTTTGGG CACTAGCAAC AAAAAGAGGA GTTTGTTCCA GATGTTCAAG 
ACAACAAAAA CAGCTGGCGG GACTAGGCTT CTTCGCGCAA ACTTGTTACA GCCTCTGAAA 
GATATTGAAA CTATTAACAC TCGCCTGGAC TGCCTGGATG AGTTGATGAG CAATGAACAA 
CTATTCTTTG GGCTTTCACA AGTTTTACGA AAATTTCCAA AAGAGACTGA TCGAGTTCTT 
TGTCATTTCT GCTTCAAGCC AAAGAAAGTC ACTGAAGCGG TCATAGGTTT TGAAAATACC 
AGAAAGAGCC AAAACATGAT TTCAAGCATT ATCTTACTTA AGACTGCATT GGATGCTTTA 
CCTATACTTG CAAAGGTTCT GAAGGACGCA AAGTGTTTTC TTCTTGCCAA TGTTTACAAG 
TCAGTGTGTG AAAATGATAG ATATGCTTCC ATCAGAAAAA AAATCGGGGA AGTTATTGAT 
GATGATGTCC TCCATGCACG GGTTCCGTTT GTTGCCCGTA CACAGCAGTG TTTTGCTTTA 
AAAGCCGGAA TTGATGGATT TTTGGATATT GCAAGAAGAA CCTTCTGTGA TACTAGTGAA 
GCAATACATA ATCTGGCCAG TAAGTATCGT GAGGAATTCA ACTTGCCAAA TTTGAAACTC 
CCTTTCAACA ACAGGCAGGG ATTCTTTTTC AGAATTCCTC AGAAGGAAGT TCAGGGAAAA 
CTTCCGAACA AATTCACTCA GGTTGTAAAA CATGGGAAAA ACATACATTG TTCGAGTCTG 
GAACTGGCTT CTTTGAATGT CAGGAATAAG TCTGCCGCTG GAGAGTGTTT CATTCGAACG 
GAAACATGCT TGGAAGCACT AATGGATGCT ATAAGGGAAG ATATCTCTGC CCTCACACTC 
CTAGCAGAAG TCTTATGTCT CCTAGATATG ATTGTTAATT CATTTGCTCA TACAATATCC 
ACAAAGCCAG TGGATCGATA CTCTAGACCT GAACTAACAG ATAGTGGCCC ACTGGCAATT 
GATGCTGGAA GGCATCCCAT CCTAGAAAGC ATACACAACG ACTTTGTTTC TAATAGTATA 
TTCATGTCAG AAGCAACCAA CATGTTGGTA GTTATGGGTC CAAACATGAG CGGAAAGAGC 
ACATATCTCC AACAGGTGTG TCTAGTAGTC ATTCTTGCTC AGATTGGTTG CTATGTCCCA 
GCTCGCTTTG CAACTATAAG GGTGGTTGAC CGCATATTTA CAAGAATGGG GACAATGGAT 
AACCTTGAGT CGAATTCCAG CACGTTTATG ACGGAGATGA GAGAGACCGC TTTCATAATG 
CAGAATGTCA CCAATAGGAG TCTGATAGTC ATGGATGAAC TCGGGAGGGC TACTTCTTCC 
TCAGACGGGT TAGCAATGGC ATGGAGCTGC TGCGAGTATC TCTTATCACT CAAAGCGTAC 
ACAGTATTTG CAACCCATAT GGACAGTCTC GCAGAGTTAG CAACCATCTA CCCAAATGTC 
AAGGTTCTTC ATTTTTATGT AGACATCAGA GATAACCGCT TAGACTTCAA GGTTCTGTCT 
GTCTTCCTTT CGTATGAGTT CGAGACTTTA GGTACTGCTG CTGAATGTTA CATTGTTCTG 
ATCAGTTTCA ACTGCGAGAT GGAACCCTAC ATGTTCCACA TTATGGCCTT CTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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MSH4 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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