SPK1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPK1
Entrez Gene ID	827328
Full Name	guanyl-nucleotide exchange factors;GTPase binding;GTP binding protein
Synonyms	DL4200C,FCAALL.346,SPIKE1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001341097.1	NP_001319958.1	guanyl-nucleotide exchange factors;GTPase binding;GTP binding protein
NM_117729.8	NP_193367.7	guanyl-nucleotide exchange factors;GTPase binding;GTP binding protein

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DOCK7	XP_005620391.1
Bos taurus (cattle)	DOCK7	NP_001179835.2
Danio rerio (zebrafish)	dock7	XP_005165943.1
Caenorhabditis elegans (roundworm)	F46H5.4	NP_509219.1
Mus musculus (house mouse)	Dock7	NP_080358.3
Rattus norvegicus (Norway rat)	Dock7	NP_001178503.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010192	XP_319370.4
Arabidopsis thaliana (thale cress)	SPK1	NP_193367.7
Homo sapiens (human)	DOCK7	NP_212132.2
	DOCK7	XP_001085641.2
Gallus gallus (chicken)	DOCK7	XP_004936918.1
Drosophila melanogaster (fruit fly)	Zir	NP_608489.2
Xenopus tropicalis (tropical clawed frog)	dock6	XP_004913993.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we demonstrated that SPIKE1 and Rho of Plants (ROP) GTPases were required for the final shape formation of conical cells, as KTN1 does. Live-cell imaging showed that wild-type cells exhibited random orientation of cortical microtubule arrays at early developmental stages but displayed a well-ordered circumferential orientation of microtubule arrays at later stages.
Title: Spatio-temporal orientation of microtubules controls conical cell shape in Arabidopsis thaliana petals.

Results suggest that during the late petal development stages, SPIKE1 (SPK1), encoding a guanine nucleotide exchange factor and Rho of Plants (ROP) GTPase proteins (ROP2, ROP4, and ROP6)-dependent signaling module influences anisotropic growth in the petal and defines the final organ shape.
Title: SPIKE1 Activates ROP GTPase to Modulate Petal Growth and Shape.

Data show that SPIKE1 is a peripheral membrane protein that accumulates at, and promotes the formation of, a specialized domain of the endoplasmic reticulum (ER) termed the ER exit site (ERES).
Title: SPIKE1 signals originate from and assemble specialized domains of the endoplasmic reticulum.

The DOCK family protein SPIKE1 (SPK1) is a GEF, and that one function of SPK1 is to control actin polymerization via two heteromeric complexes termed WAVE and actin-related protein (ARP) 2/3.
Title: A SPIKE1 signaling complex controls actin-dependent cell morphogenesis through the heteromeric WAVE and ARP2/3 complexes.

The following SPK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb15084	NM_117729.8 Latest version!	Arabidopsis thaliana guanyl-nucleotide exchange factors;GTPase binding;GTP binding protein (SPK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OAb15084	NM_001341097.1 Latest version!	Arabidopsis thaliana guanyl-nucleotide exchange factors;GTPase binding;GTP binding protein (SPK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPK1 ( NM_001341097.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001319958.1 Arabidopsis thaliana guanyl-nucleotide exchange factors;GTPase binding;GTP binding protein (SPK1), mRNA.

Price & Availability↑

CloneID	OAb15084
Clone ID Related Accession (Same CDS sequence)	NM_001341097.1 , NM_117729.8
Accession Version	NM_001341097.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	guanyl-nucleotide exchange factors;GTPase binding;GTP binding protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGGAGAACA ACAATCTTGG TCTTCGATTT CGTAAGCTAC CTCGTCAGCC TCTTGCTCTC 
CCTAAGCTCG ATCCTTTGCT TGATGAGAAT CTGGAACAAT GGCCACATTT GAATCAATTG 
GTTCAATGTT ATGGCACTGA ATGGGTTAAA GATGTTAACA AATACGGTCA CTATGAAAAT 
ATTCGACCTG ATTCTTTCCA GACTCAGATT TTTGAAGGAC CTGATACTGA TACTGAGACT 
GAGATTCGTC TTGCTAGTGC CAGGAGTGCA ACTATTGAGG AGGATGTTGC CAGTATTTCC 
GGGAGGCCGT TTTCTGATCC TGGATCCTCT AAGCATTTTG GGCAACCTCC TTTACCTGCT 
TATGAACCAG CTTTTGACTG GGAAAATGAA AGAGCTATGA TTTTTGGTCA GAGAACTCCT 
GAATCTCCTG CAGCTAGCTA TTCCAGTGGA TTGAAGATCT CTGTCAGAGT TTTGTCTCTA 
GCTTTTCAGT CTGGCTTAGT TGAACCATTT TTTGGCTCGA TTGCATTGTA CAATCAAGAG 
AGGAAGGAGA AACTCTCTGA GGATTTTTAT TTCCAGATTC AGCCGACGGA AATGCAGGAT 
GCTAAACTTT CATCTGAAAA TCGTGGAGTA TTTTATCTAG ATGCCCCATC AGCATCAGTT 
TGCCTGCTTA TTCAATTAGA AAAAACAGCA ACTGAGGAAG GAGGCGTTAC ATCATCTGTC 
TATTCCCGTA AAGAGCCTGT GCACTTAACT GAGAGGGAAA AGCAAAAGTT GCAGGTCTGG 
TCTCGCATTA TGCCTTACCG AGAGTCTTTT GCTTGGGCAG TTGTTCCGCT TTTTGATAAT 
AATCTCACCA CAAACACTGG TGAATCTGCT TCCCCTAGCA GTCCTCTTGC TCCTAGTATG 
ACTGCGTCAA GTTCCCATGA TGGTGTTTAT GAACCAATTG CAAAGATCAC ATCAGATGGA 
AAGCAAGGTT ATTCAGGTGG AAGTTCTGTC GTAGTTGAAA TATCTAATTT AAATAAAGTT 
AAAGAGAGCT ACTCTGAAGA GTCCATTCAG GACCCCAAAC GGAAGGTTCA CAAACCTGTT 
AAAGGGGTTC TAAGACTGGA AATTGAGAAA CATCGGAATG GACATGGAGA CTTTGAAGAT 
CTATCTGAGA ATGGAAGTAT CATAAATGAC TCCCTTGATC CAACTGACCG CCTCAGTGAC 
CTGACTCTTA TGAAGTGTCC CAGTTCTAGT TCTGGTGGAC CCCGTAATGG CTGTTCAAAG 
TGGAATTCAG AGGATGCAAA GGATGTTTCG AGAAATCTAA CAAGTTCATG TGGGACTCCT 
GACTTAAATT GTTATCATGC TTTTGATTTT TGCTCTACGA CAAGAAACGA GCCTTTTCTA 
CATCTCTTCC ATTGCCTTTA TGTGTATCCT GTAGCTGTTA CTTTAAGTCG GAAGAGGAAT 
CCCTTTATCC GTGTAGAATT GAGAAAGGAT GACACAGATA TCCGAAAACA ACCACTCGAG 
GCAATATATC CAAGAGAACC TGGTGTTTCA CTCCAGAAAT GGGTTCATAC ACAGGTCGCT 
GTTGGTGCTA GGGCCGCTAG CTACCATGAT GAAATTAAGG TCTCTCTTCC TGCCACATGG 
ACGCCATCAC ATCATCTGTT ATTCACTTTT TTTCATGTTG ATCTCCAGAC AAAGCTTGAA 
GCTCCAAGAC CAGTAGTTGT TGGATATGCA TCACTTCCAT TATCTACATA TATCCACTCA 
CGGTCTGATA TTTCTCTACC AGTAATGAGA GAACTTGTTC CCCACTATCT TCAGGAAAGC 
ACCAAGGAGA GGTTGGATTA TTTGGAAGAT GGCAAAAATA TTTTTAAGTT GCGACTGAGA 
CTTTGTTCAT CTCTTTATCC CACCAACGAA CGTGTCAGGG ATTTCTGTCT AGAGTATGAT 
AGACATACCC TCCAGACAAG GCCTCCCTGG GGTTCAGAAC TGTTGCAGGC AATAAACAGT 
TTGAAGCACG TTGATTCTAC TGCCTTGCTT CAGTTTCTTT ACCCAATTCT TAACATGCTC 
CTTCATCTTA TTGGCAATGG TGGAGAAACC CTTCAGGTTG CAGCATTCAG AGCCATGGTG 
GATATTTTAA CTCGGGTGCA GCAGGTGTCG TTTGATGATG CTGACCGAAA TCGATTTCTG 
GTCACCTATG TTGATTATTC TTTTGATGAT TTTGGAGGCA ATCAACCACC GGTTTATCCT 
GGATTAGCAA CTGTGTGGGG AAGTTTAGCT AGAAGCAAGG CCAAAGGTTA CCGGGTTGGA 
CCTGTATATG ACGATGTATT ATCAATGGCG TGGTTTTTCC TCGAGTTGAT TGTTAAATCA 
ATGGCATTGG AGCAAGCACG TCTTTATGAT CACAATCTAC CTACAGGCGA GGATGTTCCA 
CCCATGCAGC TCAAAGAAAG TGTTTTCCGA TGTATCATGC AATTGTTTGA TTGTCTTTTA 
ACTGAAGTAC ATGAACGTTG TAAAAAGGGC TTAAGCTTGG CGAAACGTCT GAACAGCAGT 
TTGGCCTTCT TCTGTTATGA TCTTTTATAT ATCATTGAGC CCTGCCAGGT CTATGAACTG 
GTATCGTTAT ACATGGACAA GTTTTCTGGT GTTTGTCAAT CTGTTCTACA CGAATGCAAG 
CTCACGTTTT TGCAAATTAT CTCCGACCAT GATCTTTTTG TAGAAATGCC TGGTAGAGAC 
CCATCAGACA GGAACTATTT ATCATCCATA TTAATACAGG AGTTATTTCT CTCACTGGAT 
CATGATGAGC TGCCTCTGCG GGCGAAGGGA GCAAGAATTT TAGTGATCCT TTTATGCAAG 
CATGAATTTG ATGCGCGGTA CCAGAAAGCT GAAGATAAGC TGTATATTGC ACAACTGTAT 
TTTCCTTTTG TCGGGCAGAT TTTAGATGAA ATGCCTGTTT TTTATAACCT AAATGCTACT 
GAAAAGCGTG AGGTTTTAAT TGGTGTGCTG CAAATTGTTC GTAATCTGGA TGACACATCA 
CTTGTCAAAG CATGGCAGCA GAGCATCGCT CGTACTCGAT TATATTTCAA ACTCATGGAA 
GAATGCCTCA TACTTTTCGA GCACAAGAAG GCTGCTGACA GCATCCTCGG GGGCAACAAT 
TCTCGTGGTC CTGTTAGTGA AGGAGCTGGA TCACCAAAGT ACTCCGAGCG ACTTTCTCCT 
GCTATCAACA ATTATCTGTC AGAGGCTTCT CGCCAAGAAG TCAGGCTAGA GGGAACACCT 
GATAATGGGT ACCTGTGGCA GAGAGTTAAT TCTCAGTTGG CCTCCCCTAG CCAACCTTAT 
TCTCTAAGAG AAGCTTTAGC TCAAGCACAA TCTTCCCGGA TTGGAGCTTC AGCCCAGGCA 
CTAAGAGAAT CGCTGCACCC AATTTTGAGG CAAAAACTGG AACTTTGGGA GGAGAATGTA 
AGTGCCACTG TTAGCCTCCA GGTTTTGGAG ATCACTGAAA ATTTTTCATC AATGGCAGCC 
TCTCACAATA TTGCGACCGA CTATGGCAAA TTGGACTGCA TTACTACAAT ACTGACGAGT 
TTCTTCTCCC GAAACCAGTC ATTAGCTTTC TGGAAAGCCT TCTTTCCAAT TTTCAACAGA 
ATCTTTGATC TTCATGGGGC TACACTGATG GCCAGGGAAA ATGATCGCTT CTTAAAACAG 
ATAGCCTTCC ATCTTCTCCG GCTTGCTGTT TACCGCAATG ACAGTGTCAG GAAAAGGGCT 
GTTATTGGTC TTCAAATACT AGTTAAGAGC TCGCTATACT TTATGCAGAC TGCTAGGCTG 
AGGGCTTTGC TGACGATTAC ACTGTCAGAA CTCATGTCGG ATGTCCAAGT GACTCATATG 
AAGTCAGATA ATACATTAGA AGAGAGTGGA GAAGCACGGC GACTTCAACA GTCATTAAGC 
GAAATGGCTG ATGAAGCTAA GAGTGTCAAT TTGTTGAGGG AGTGTGGTCT TCCTGATGAT 
ACACTGTTGA TTATTCCCGA GAAATTTACA GAGAACCGCT GGTCATGGGC TGAAGTCAAA 
CATCTTTCTG ACAGCCTTGT TCTTGCTCTT GATGCCAGCC TCGGGCATGC CCTTTTGGGA 
TCTGTGATGG CTATGGATCG ATATGCTGCT GCAGAGAGCT TCTACAAACT TGGAATGGCG 
TTTGCTCCTG TTCCGGATCT TCACATAATG TGGTTATTGC ATCTATGTGA TGCCCACCAG 
GAAATGCAGT CTTGGGCAGA AGCTGCACAG TGTGCTGTGG CGGTTGCAGG TGTGATAATG 
CAGGCCTTGG TGGCTAGGAA TGATGGTGTG TGGAGCAAGG ATCATGTGTC TGCCTTGCGT 
AAGATTTGCC CGATGGTAAG TGGCGAGTTT ACAACAGAGG CATCTGCAGC TGAAGTCGAG 
GGTTATGGTG CTTCAAAGCT AACAGTTGAC TCCGCCGTCA AGTATTTACA GCTTGCTAAT 
AAGCTTTTCT CTCAAGCTGA GCTCTACCAT TTTTGTGCAA GCATTTTAGA ACTTGTTATT 
CCAGTTTACA AGAGCAGGAA GGCTTATGGG CAGTTGGCTA AGTGTCACAC TTTACTTACT 
AATATATACG AGTCCATTCT TGACCAAGAA TCAAACCCAA TCCCATTTAT TGATGCTACA 
TATTACCGGG TGGGATTTTA TGGGGAAAAG TTTGGGAAGT TGGACAGGAA AGAATATGTA 
TACCGAGAAC CACGAGATGT GCGGCTAGGG GATATAATGG AAAAACTGAG CCATATATAT 
GAGTCAAGAA TGGACAGCAA CCATATTCTG CACATTATAC CAGATTCGAG GCAAGTGAAG 
GCAGAAGACT TGCAGGCAGG AGTGTGCTAT CTGCAAATTA CTGCTGTTGA TGCAGTGATG 
GAAGATGAGG ATCTTGGGAG CAGAAGAGAG AGAATATTTT CTCTTTCAAC TGGAAGTGTT 
CGAGCACGAG TGTTTGATCG TTTCTTATTT GACACACCAT TTACGAAAAA TGGTAAAACC 
CAAGGTGGAT TAGAAGATCA ATGGAAGAGA AGAACAGTGC TGCAGACTGA GGGTTCATTC 
CCTGCCCTTG TGAATAGGCT GTTGGTTACC AAGTCTGAAT CCCTTGAATT CTCGCCAGTG 
GAGAATGCGA TTGGAATGAT TGAAACGCGG ACAACCGCTC TGAGAAATGA GCTAGAGGAG 
CCTCGTAGCT CGGATGGGGA TCACCTACCA CGGCTTCAAA GTCTGCAGAG GATTCTCCAA 
GGAAGTGTTG CAGTGCAGGT GAACAGTGGG GTGTTGAGCG TGTGCACAGC TTTCTTATCA 
GGAGAGCCTG CAACGAGATT GCGTTCACAG GAACTGCAGC AACTAATCGC GGCACTGCTT 
GAATTCATGG CGGTTTGTAA GCGAGCCATT AGGGTTCACT TTAGATTAAT CGGAGAAGAA 
GATCAGGAAT TCCATACTCA GCTTGTCAAT GGATTTCAGT CTCTCACCGC AGAACTCTCC 
CATTACATCC CTGCTATCTT ATCCGAACTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001319958.1
CDS	373..5865
Translation	MENNNLGLRFRKLPRQPLALPKLDPLLDENLEQWPHLNQLVQCYGTEWVKDVNKYGHYENIRPDSFQTQIFEGPDTDTETEIRLASARSATIEEDVASISGRPFSDPGSSKHFGQPPLPAYEPAFDWENERAMIFGQRTPESPAASYSSGLKISVRVLSLAFQSGLVEPFFGSIALYNQERKEKLSEDFYFQIQPTEMQDAKLSSENRGVFYLDAPSASVCLLIQLEKTATEEGGVTSSVYSRKEPVHLTEREKQKLQVWSRIMPYRESFAWAVVPLFDNNLTTNTGESASPSSPLAPSMTASSSHDGVYEPIAKITSDGKQGYSGGSSVVVEISNLNKVKESYSEESIQDPKRKVHKPVKGVLRLEIEKHRNGHGDFEDLSENGSIINDSLDPTDRLSDLTLMKCPSSSSGGPRNGCSKWNSEDAKDVSRNLTSSCGTPDLNCYHAFDFCSTTRNEPFLHLFHCLYVYPVAVTLSRKRNPFIRVELRKDDTDIRKQPLEAIYPREPGVSLQKWVHTQVAVGARAASYHDEIKVSLPATWTPSHHLLFTFFHVDLQTKLEAPRPVVVGYASLPLSTYIHSRSDISLPVMRELVPHYLQESTKERLDYLEDGKNIFKLRLRLCSSLYPTNERVRDFCLEYDRHTLQTRPPWGSELLQAINSLKHVDSTALLQFLYPILNMLLHLIGNGGETLQVAAFRAMVDILTRVQQVSFDDADRNRFLVTYVDYSFDDFGGNQPPVYPGLATVWGSLARSKAKGYRVGPVYDDVLSMAWFFLELIVKSMALEQARLYDHNLPTGEDVPPMQLKESVFRCIMQLFDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLYIIEPCQVYELVSLYMDKFSGVCQSVLHECKLTFLQIISDHDLFVEMPGRDPSDRNYLSSILIQELFLSLDHDELPLRAKGARILVILLCKHEFDARYQKAEDKLYIAQLYFPFVGQILDEMPVFYNLNATEKREVLIGVLQIVRNLDDTSLVKAWQQSIARTRLYFKLMEECLILFEHKKAADSILGGNNSRGPVSEGAGSPKYSERLSPAINNYLSEASRQEVRLEGTPDNGYLWQRVNSQLASPSQPYSLREALAQAQSSRIGASAQALRESLHPILRQKLELWEENVSATVSLQVLEITENFSSMAASHNIATDYGKLDCITTILTSFFSRNQSLAFWKAFFPIFNRIFDLHGATLMARENDRFLKQIAFHLLRLAVYRNDSVRKRAVIGLQILVKSSLYFMQTARLRALLTITLSELMSDVQVTHMKSDNTLEESGEARRLQQSLSEMADEAKSVNLLRECGLPDDTLLIIPEKFTENRWSWAEVKHLSDSLVLALDASLGHALLGSVMAMDRYAAAESFYKLGMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAGVIMQALVARNDGVWSKDHVSALRKICPMVSGEFTTEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCASILELVIPVYKSRKAYGQLAKCHTLLTNIYESILDQESNPIPFIDATYYRVGFYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHIYESRMDSNHILHIIPDSRQVKAEDLQAGVCYLQITAVDAVMEDEDLGSRRERIFSLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTTALRNELEEPRSSDGDHLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAVCKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL

Target ORF information:

RefSeq Version	NM_001341097.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana guanyl-nucleotide exchange factors;GTPase binding;GTP binding protein (SPK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001341097.1

ORF Insert Sequence:

ATGGAGAACA ACAATCTTGG TCTTCGATTT CGTAAGCTAC CTCGTCAGCC TCTTGCTCTC 
CCTAAGCTCG ATCCTTTGCT TGATGAGAAT CTGGAACAAT GGCCACATTT GAATCAATTG 
GTTCAATGTT ATGGCACTGA ATGGGTTAAA GATGTTAACA AATACGGTCA CTATGAAAAT 
ATTCGACCTG ATTCTTTCCA GACTCAGATT TTTGAAGGAC CTGATACTGA TACTGAGACT 
GAGATTCGTC TTGCTAGTGC CAGGAGTGCA ACTATTGAGG AGGATGTTGC CAGTATTTCC 
GGGAGGCCGT TTTCTGATCC TGGATCCTCT AAGCATTTTG GGCAACCTCC TTTACCTGCT 
TATGAACCAG CTTTTGACTG GGAAAATGAA AGAGCTATGA TTTTTGGTCA GAGAACTCCT 
GAATCTCCTG CAGCTAGCTA TTCCAGTGGA TTGAAGATCT CTGTCAGAGT TTTGTCTCTA 
GCTTTTCAGT CTGGCTTAGT TGAACCATTT TTTGGCTCGA TTGCATTGTA CAATCAAGAG 
AGGAAGGAGA AACTCTCTGA GGATTTTTAT TTCCAGATTC AGCCGACGGA AATGCAGGAT 
GCTAAACTTT CATCTGAAAA TCGTGGAGTA TTTTATCTAG ATGCCCCATC AGCATCAGTT 
TGCCTGCTTA TTCAATTAGA AAAAACAGCA ACTGAGGAAG GAGGCGTTAC ATCATCTGTC 
TATTCCCGTA AAGAGCCTGT GCACTTAACT GAGAGGGAAA AGCAAAAGTT GCAGGTCTGG 
TCTCGCATTA TGCCTTACCG AGAGTCTTTT GCTTGGGCAG TTGTTCCGCT TTTTGATAAT 
AATCTCACCA CAAACACTGG TGAATCTGCT TCCCCTAGCA GTCCTCTTGC TCCTAGTATG 
ACTGCGTCAA GTTCCCATGA TGGTGTTTAT GAACCAATTG CAAAGATCAC ATCAGATGGA 
AAGCAAGGTT ATTCAGGTGG AAGTTCTGTC GTAGTTGAAA TATCTAATTT AAATAAAGTT 
AAAGAGAGCT ACTCTGAAGA GTCCATTCAG GACCCCAAAC GGAAGGTTCA CAAACCTGTT 
AAAGGGGTTC TAAGACTGGA AATTGAGAAA CATCGGAATG GACATGGAGA CTTTGAAGAT 
CTATCTGAGA ATGGAAGTAT CATAAATGAC TCCCTTGATC CAACTGACCG CCTCAGTGAC 
CTGACTCTTA TGAAGTGTCC CAGTTCTAGT TCTGGTGGAC CCCGTAATGG CTGTTCAAAG 
TGGAATTCAG AGGATGCAAA GGATGTTTCG AGAAATCTAA CAAGTTCATG TGGGACTCCT 
GACTTAAATT GTTATCATGC TTTTGATTTT TGCTCTACGA CAAGAAACGA GCCTTTTCTA 
CATCTCTTCC ATTGCCTTTA TGTGTATCCT GTAGCTGTTA CTTTAAGTCG GAAGAGGAAT 
CCCTTTATCC GTGTAGAATT GAGAAAGGAT GACACAGATA TCCGAAAACA ACCACTCGAG 
GCAATATATC CAAGAGAACC TGGTGTTTCA CTCCAGAAAT GGGTTCATAC ACAGGTCGCT 
GTTGGTGCTA GGGCCGCTAG CTACCATGAT GAAATTAAGG TCTCTCTTCC TGCCACATGG 
ACGCCATCAC ATCATCTGTT ATTCACTTTT TTTCATGTTG ATCTCCAGAC AAAGCTTGAA 
GCTCCAAGAC CAGTAGTTGT TGGATATGCA TCACTTCCAT TATCTACATA TATCCACTCA 
CGGTCTGATA TTTCTCTACC AGTAATGAGA GAACTTGTTC CCCACTATCT TCAGGAAAGC 
ACCAAGGAGA GGTTGGATTA TTTGGAAGAT GGCAAAAATA TTTTTAAGTT GCGACTGAGA 
CTTTGTTCAT CTCTTTATCC CACCAACGAA CGTGTCAGGG ATTTCTGTCT AGAGTATGAT 
AGACATACCC TCCAGACAAG GCCTCCCTGG GGTTCAGAAC TGTTGCAGGC AATAAACAGT 
TTGAAGCACG TTGATTCTAC TGCCTTGCTT CAGTTTCTTT ACCCAATTCT TAACATGCTC 
CTTCATCTTA TTGGCAATGG TGGAGAAACC CTTCAGGTTG CAGCATTCAG AGCCATGGTG 
GATATTTTAA CTCGGGTGCA GCAGGTGTCG TTTGATGATG CTGACCGAAA TCGATTTCTG 
GTCACCTATG TTGATTATTC TTTTGATGAT TTTGGAGGCA ATCAACCACC GGTTTATCCT 
GGATTAGCAA CTGTGTGGGG AAGTTTAGCT AGAAGCAAGG CCAAAGGTTA CCGGGTTGGA 
CCTGTATATG ACGATGTATT ATCAATGGCG TGGTTTTTCC TCGAGTTGAT TGTTAAATCA 
ATGGCATTGG AGCAAGCACG TCTTTATGAT CACAATCTAC CTACAGGCGA GGATGTTCCA 
CCCATGCAGC TCAAAGAAAG TGTTTTCCGA TGTATCATGC AATTGTTTGA TTGTCTTTTA 
ACTGAAGTAC ATGAACGTTG TAAAAAGGGC TTAAGCTTGG CGAAACGTCT GAACAGCAGT 
TTGGCCTTCT TCTGTTATGA TCTTTTATAT ATCATTGAGC CCTGCCAGGT CTATGAACTG 
GTATCGTTAT ACATGGACAA GTTTTCTGGT GTTTGTCAAT CTGTTCTACA CGAATGCAAG 
CTCACGTTTT TGCAAATTAT CTCCGACCAT GATCTTTTTG TAGAAATGCC TGGTAGAGAC 
CCATCAGACA GGAACTATTT ATCATCCATA TTAATACAGG AGTTATTTCT CTCACTGGAT 
CATGATGAGC TGCCTCTGCG GGCGAAGGGA GCAAGAATTT TAGTGATCCT TTTATGCAAG 
CATGAATTTG ATGCGCGGTA CCAGAAAGCT GAAGATAAGC TGTATATTGC ACAACTGTAT 
TTTCCTTTTG TCGGGCAGAT TTTAGATGAA ATGCCTGTTT TTTATAACCT AAATGCTACT 
GAAAAGCGTG AGGTTTTAAT TGGTGTGCTG CAAATTGTTC GTAATCTGGA TGACACATCA 
CTTGTCAAAG CATGGCAGCA GAGCATCGCT CGTACTCGAT TATATTTCAA ACTCATGGAA 
GAATGCCTCA TACTTTTCGA GCACAAGAAG GCTGCTGACA GCATCCTCGG GGGCAACAAT 
TCTCGTGGTC CTGTTAGTGA AGGAGCTGGA TCACCAAAGT ACTCCGAGCG ACTTTCTCCT 
GCTATCAACA ATTATCTGTC AGAGGCTTCT CGCCAAGAAG TCAGGCTAGA GGGAACACCT 
GATAATGGGT ACCTGTGGCA GAGAGTTAAT TCTCAGTTGG CCTCCCCTAG CCAACCTTAT 
TCTCTAAGAG AAGCTTTAGC TCAAGCACAA TCTTCCCGGA TTGGAGCTTC AGCCCAGGCA 
CTAAGAGAAT CGCTGCACCC AATTTTGAGG CAAAAACTGG AACTTTGGGA GGAGAATGTA 
AGTGCCACTG TTAGCCTCCA GGTTTTGGAG ATCACTGAAA ATTTTTCATC AATGGCAGCC 
TCTCACAATA TTGCGACCGA CTATGGCAAA TTGGACTGCA TTACTACAAT ACTGACGAGT 
TTCTTCTCCC GAAACCAGTC ATTAGCTTTC TGGAAAGCCT TCTTTCCAAT TTTCAACAGA 
ATCTTTGATC TTCATGGGGC TACACTGATG GCCAGGGAAA ATGATCGCTT CTTAAAACAG 
ATAGCCTTCC ATCTTCTCCG GCTTGCTGTT TACCGCAATG ACAGTGTCAG GAAAAGGGCT 
GTTATTGGTC TTCAAATACT AGTTAAGAGC TCGCTATACT TTATGCAGAC TGCTAGGCTG 
AGGGCTTTGC TGACGATTAC ACTGTCAGAA CTCATGTCGG ATGTCCAAGT GACTCATATG 
AAGTCAGATA ATACATTAGA AGAGAGTGGA GAAGCACGGC GACTTCAACA GTCATTAAGC 
GAAATGGCTG ATGAAGCTAA GAGTGTCAAT TTGTTGAGGG AGTGTGGTCT TCCTGATGAT 
ACACTGTTGA TTATTCCCGA GAAATTTACA GAGAACCGCT GGTCATGGGC TGAAGTCAAA 
CATCTTTCTG ACAGCCTTGT TCTTGCTCTT GATGCCAGCC TCGGGCATGC CCTTTTGGGA 
TCTGTGATGG CTATGGATCG ATATGCTGCT GCAGAGAGCT TCTACAAACT TGGAATGGCG 
TTTGCTCCTG TTCCGGATCT TCACATAATG TGGTTATTGC ATCTATGTGA TGCCCACCAG 
GAAATGCAGT CTTGGGCAGA AGCTGCACAG TGTGCTGTGG CGGTTGCAGG TGTGATAATG 
CAGGCCTTGG TGGCTAGGAA TGATGGTGTG TGGAGCAAGG ATCATGTGTC TGCCTTGCGT 
AAGATTTGCC CGATGGTAAG TGGCGAGTTT ACAACAGAGG CATCTGCAGC TGAAGTCGAG 
GGTTATGGTG CTTCAAAGCT AACAGTTGAC TCCGCCGTCA AGTATTTACA GCTTGCTAAT 
AAGCTTTTCT CTCAAGCTGA GCTCTACCAT TTTTGTGCAA GCATTTTAGA ACTTGTTATT 
CCAGTTTACA AGAGCAGGAA GGCTTATGGG CAGTTGGCTA AGTGTCACAC TTTACTTACT 
AATATATACG AGTCCATTCT TGACCAAGAA TCAAACCCAA TCCCATTTAT TGATGCTACA 
TATTACCGGG TGGGATTTTA TGGGGAAAAG TTTGGGAAGT TGGACAGGAA AGAATATGTA 
TACCGAGAAC CACGAGATGT GCGGCTAGGG GATATAATGG AAAAACTGAG CCATATATAT 
GAGTCAAGAA TGGACAGCAA CCATATTCTG CACATTATAC CAGATTCGAG GCAAGTGAAG 
GCAGAAGACT TGCAGGCAGG AGTGTGCTAT CTGCAAATTA CTGCTGTTGA TGCAGTGATG 
GAAGATGAGG ATCTTGGGAG CAGAAGAGAG AGAATATTTT CTCTTTCAAC TGGAAGTGTT 
CGAGCACGAG TGTTTGATCG TTTCTTATTT GACACACCAT TTACGAAAAA TGGTAAAACC 
CAAGGTGGAT TAGAAGATCA ATGGAAGAGA AGAACAGTGC TGCAGACTGA GGGTTCATTC 
CCTGCCCTTG TGAATAGGCT GTTGGTTACC AAGTCTGAAT CCCTTGAATT CTCGCCAGTG 
GAGAATGCGA TTGGAATGAT TGAAACGCGG ACAACCGCTC TGAGAAATGA GCTAGAGGAG 
CCTCGTAGCT CGGATGGGGA TCACCTACCA CGGCTTCAAA GTCTGCAGAG GATTCTCCAA 
GGAAGTGTTG CAGTGCAGGT GAACAGTGGG GTGTTGAGCG TGTGCACAGC TTTCTTATCA 
GGAGAGCCTG CAACGAGATT GCGTTCACAG GAACTGCAGC AACTAATCGC GGCACTGCTT 
GAATTCATGG CGGTTTGTAA GCGAGCCATT AGGGTTCACT TTAGATTAAT CGGAGAAGAA 
GATCAGGAAT TCCATACTCA GCTTGTCAAT GGATTTCAGT CTCTCACCGC AGAACTCTCC 
CATTACATCC CTGCTATCTT ATCCGAACTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPK1 ( NM_117729.8 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_193367.7 Arabidopsis thaliana guanyl-nucleotide exchange factors;GTPase binding;GTP binding protein (SPK1), mRNA.

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CloneID	OAb15084
Clone ID Related Accession (Same CDS sequence)	NM_001341097.1 , NM_117729.8
Accession Version	NM_117729.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	guanyl-nucleotide exchange factors;GTPase binding;GTP binding protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_117729.7.

ATGGAGAACA ACAATCTTGG TCTTCGATTT CGTAAGCTAC CTCGTCAGCC TCTTGCTCTC 
CCTAAGCTCG ATCCTTTGCT TGATGAGAAT CTGGAACAAT GGCCACATTT GAATCAATTG 
GTTCAATGTT ATGGCACTGA ATGGGTTAAA GATGTTAACA AATACGGTCA CTATGAAAAT 
ATTCGACCTG ATTCTTTCCA GACTCAGATT TTTGAAGGAC CTGATACTGA TACTGAGACT 
GAGATTCGTC TTGCTAGTGC CAGGAGTGCA ACTATTGAGG AGGATGTTGC CAGTATTTCC 
GGGAGGCCGT TTTCTGATCC TGGATCCTCT AAGCATTTTG GGCAACCTCC TTTACCTGCT 
TATGAACCAG CTTTTGACTG GGAAAATGAA AGAGCTATGA TTTTTGGTCA GAGAACTCCT 
GAATCTCCTG CAGCTAGCTA TTCCAGTGGA TTGAAGATCT CTGTCAGAGT TTTGTCTCTA 
GCTTTTCAGT CTGGCTTAGT TGAACCATTT TTTGGCTCGA TTGCATTGTA CAATCAAGAG 
AGGAAGGAGA AACTCTCTGA GGATTTTTAT TTCCAGATTC AGCCGACGGA AATGCAGGAT 
GCTAAACTTT CATCTGAAAA TCGTGGAGTA TTTTATCTAG ATGCCCCATC AGCATCAGTT 
TGCCTGCTTA TTCAATTAGA AAAAACAGCA ACTGAGGAAG GAGGCGTTAC ATCATCTGTC 
TATTCCCGTA AAGAGCCTGT GCACTTAACT GAGAGGGAAA AGCAAAAGTT GCAGGTCTGG 
TCTCGCATTA TGCCTTACCG AGAGTCTTTT GCTTGGGCAG TTGTTCCGCT TTTTGATAAT 
AATCTCACCA CAAACACTGG TGAATCTGCT TCCCCTAGCA GTCCTCTTGC TCCTAGTATG 
ACTGCGTCAA GTTCCCATGA TGGTGTTTAT GAACCAATTG CAAAGATCAC ATCAGATGGA 
AAGCAAGGTT ATTCAGGTGG AAGTTCTGTC GTAGTTGAAA TATCTAATTT AAATAAAGTT 
AAAGAGAGCT ACTCTGAAGA GTCCATTCAG GACCCCAAAC GGAAGGTTCA CAAACCTGTT 
AAAGGGGTTC TAAGACTGGA AATTGAGAAA CATCGGAATG GACATGGAGA CTTTGAAGAT 
CTATCTGAGA ATGGAAGTAT CATAAATGAC TCCCTTGATC CAACTGACCG CCTCAGTGAC 
CTGACTCTTA TGAAGTGTCC CAGTTCTAGT TCTGGTGGAC CCCGTAATGG CTGTTCAAAG 
TGGAATTCAG AGGATGCAAA GGATGTTTCG AGAAATCTAA CAAGTTCATG TGGGACTCCT 
GACTTAAATT GTTATCATGC TTTTGATTTT TGCTCTACGA CAAGAAACGA GCCTTTTCTA 
CATCTCTTCC ATTGCCTTTA TGTGTATCCT GTAGCTGTTA CTTTAAGTCG GAAGAGGAAT 
CCCTTTATCC GTGTAGAATT GAGAAAGGAT GACACAGATA TCCGAAAACA ACCACTCGAG 
GCAATATATC CAAGAGAACC TGGTGTTTCA CTCCAGAAAT GGGTTCATAC ACAGGTCGCT 
GTTGGTGCTA GGGCCGCTAG CTACCATGAT GAAATTAAGG TCTCTCTTCC TGCCACATGG 
ACGCCATCAC ATCATCTGTT ATTCACTTTT TTTCATGTTG ATCTCCAGAC AAAGCTTGAA 
GCTCCAAGAC CAGTAGTTGT TGGATATGCA TCACTTCCAT TATCTACATA TATCCACTCA 
CGGTCTGATA TTTCTCTACC AGTAATGAGA GAACTTGTTC CCCACTATCT TCAGGAAAGC 
ACCAAGGAGA GGTTGGATTA TTTGGAAGAT GGCAAAAATA TTTTTAAGTT GCGACTGAGA 
CTTTGTTCAT CTCTTTATCC CACCAACGAA CGTGTCAGGG ATTTCTGTCT AGAGTATGAT 
AGACATACCC TCCAGACAAG GCCTCCCTGG GGTTCAGAAC TGTTGCAGGC AATAAACAGT 
TTGAAGCACG TTGATTCTAC TGCCTTGCTT CAGTTTCTTT ACCCAATTCT TAACATGCTC 
CTTCATCTTA TTGGCAATGG TGGAGAAACC CTTCAGGTTG CAGCATTCAG AGCCATGGTG 
GATATTTTAA CTCGGGTGCA GCAGGTGTCG TTTGATGATG CTGACCGAAA TCGATTTCTG 
GTCACCTATG TTGATTATTC TTTTGATGAT TTTGGAGGCA ATCAACCACC GGTTTATCCT 
GGATTAGCAA CTGTGTGGGG AAGTTTAGCT AGAAGCAAGG CCAAAGGTTA CCGGGTTGGA 
CCTGTATATG ACGATGTATT ATCAATGGCG TGGTTTTTCC TCGAGTTGAT TGTTAAATCA 
ATGGCATTGG AGCAAGCACG TCTTTATGAT CACAATCTAC CTACAGGCGA GGATGTTCCA 
CCCATGCAGC TCAAAGAAAG TGTTTTCCGA TGTATCATGC AATTGTTTGA TTGTCTTTTA 
ACTGAAGTAC ATGAACGTTG TAAAAAGGGC TTAAGCTTGG CGAAACGTCT GAACAGCAGT 
TTGGCCTTCT TCTGTTATGA TCTTTTATAT ATCATTGAGC CCTGCCAGGT CTATGAACTG 
GTATCGTTAT ACATGGACAA GTTTTCTGGT GTTTGTCAAT CTGTTCTACA CGAATGCAAG 
CTCACGTTTT TGCAAATTAT CTCCGACCAT GATCTTTTTG TAGAAATGCC TGGTAGAGAC 
CCATCAGACA GGAACTATTT ATCATCCATA TTAATACAGG AGTTATTTCT CTCACTGGAT 
CATGATGAGC TGCCTCTGCG GGCGAAGGGA GCAAGAATTT TAGTGATCCT TTTATGCAAG 
CATGAATTTG ATGCGCGGTA CCAGAAAGCT GAAGATAAGC TGTATATTGC ACAACTGTAT 
TTTCCTTTTG TCGGGCAGAT TTTAGATGAA ATGCCTGTTT TTTATAACCT AAATGCTACT 
GAAAAGCGTG AGGTTTTAAT TGGTGTGCTG CAAATTGTTC GTAATCTGGA TGACACATCA 
CTTGTCAAAG CATGGCAGCA GAGCATCGCT CGTACTCGAT TATATTTCAA ACTCATGGAA 
GAATGCCTCA TACTTTTCGA GCACAAGAAG GCTGCTGACA GCATCCTCGG GGGCAACAAT 
TCTCGTGGTC CTGTTAGTGA AGGAGCTGGA TCACCAAAGT ACTCCGAGCG ACTTTCTCCT 
GCTATCAACA ATTATCTGTC AGAGGCTTCT CGCCAAGAAG TCAGGCTAGA GGGAACACCT 
GATAATGGGT ACCTGTGGCA GAGAGTTAAT TCTCAGTTGG CCTCCCCTAG CCAACCTTAT 
TCTCTAAGAG AAGCTTTAGC TCAAGCACAA TCTTCCCGGA TTGGAGCTTC AGCCCAGGCA 
CTAAGAGAAT CGCTGCACCC AATTTTGAGG CAAAAACTGG AACTTTGGGA GGAGAATGTA 
AGTGCCACTG TTAGCCTCCA GGTTTTGGAG ATCACTGAAA ATTTTTCATC AATGGCAGCC 
TCTCACAATA TTGCGACCGA CTATGGCAAA TTGGACTGCA TTACTACAAT ACTGACGAGT 
TTCTTCTCCC GAAACCAGTC ATTAGCTTTC TGGAAAGCCT TCTTTCCAAT TTTCAACAGA 
ATCTTTGATC TTCATGGGGC TACACTGATG GCCAGGGAAA ATGATCGCTT CTTAAAACAG 
ATAGCCTTCC ATCTTCTCCG GCTTGCTGTT TACCGCAATG ACAGTGTCAG GAAAAGGGCT 
GTTATTGGTC TTCAAATACT AGTTAAGAGC TCGCTATACT TTATGCAGAC TGCTAGGCTG 
AGGGCTTTGC TGACGATTAC ACTGTCAGAA CTCATGTCGG ATGTCCAAGT GACTCATATG 
AAGTCAGATA ATACATTAGA AGAGAGTGGA GAAGCACGGC GACTTCAACA GTCATTAAGC 
GAAATGGCTG ATGAAGCTAA GAGTGTCAAT TTGTTGAGGG AGTGTGGTCT TCCTGATGAT 
ACACTGTTGA TTATTCCCGA GAAATTTACA GAGAACCGCT GGTCATGGGC TGAAGTCAAA 
CATCTTTCTG ACAGCCTTGT TCTTGCTCTT GATGCCAGCC TCGGGCATGC CCTTTTGGGA 
TCTGTGATGG CTATGGATCG ATATGCTGCT GCAGAGAGCT TCTACAAACT TGGAATGGCG 
TTTGCTCCTG TTCCGGATCT TCACATAATG TGGTTATTGC ATCTATGTGA TGCCCACCAG 
GAAATGCAGT CTTGGGCAGA AGCTGCACAG TGTGCTGTGG CGGTTGCAGG TGTGATAATG 
CAGGCCTTGG TGGCTAGGAA TGATGGTGTG TGGAGCAAGG ATCATGTGTC TGCCTTGCGT 
AAGATTTGCC CGATGGTAAG TGGCGAGTTT ACAACAGAGG CATCTGCAGC TGAAGTCGAG 
GGTTATGGTG CTTCAAAGCT AACAGTTGAC TCCGCCGTCA AGTATTTACA GCTTGCTAAT 
AAGCTTTTCT CTCAAGCTGA GCTCTACCAT TTTTGTGCAA GCATTTTAGA ACTTGTTATT 
CCAGTTTACA AGAGCAGGAA GGCTTATGGG CAGTTGGCTA AGTGTCACAC TTTACTTACT 
AATATATACG AGTCCATTCT TGACCAAGAA TCAAACCCAA TCCCATTTAT TGATGCTACA 
TATTACCGGG TGGGATTTTA TGGGGAAAAG TTTGGGAAGT TGGACAGGAA AGAATATGTA 
TACCGAGAAC CACGAGATGT GCGGCTAGGG GATATAATGG AAAAACTGAG CCATATATAT 
GAGTCAAGAA TGGACAGCAA CCATATTCTG CACATTATAC CAGATTCGAG GCAAGTGAAG 
GCAGAAGACT TGCAGGCAGG AGTGTGCTAT CTGCAAATTA CTGCTGTTGA TGCAGTGATG 
GAAGATGAGG ATCTTGGGAG CAGAAGAGAG AGAATATTTT CTCTTTCAAC TGGAAGTGTT 
CGAGCACGAG TGTTTGATCG TTTCTTATTT GACACACCAT TTACGAAAAA TGGTAAAACC 
CAAGGTGGAT TAGAAGATCA ATGGAAGAGA AGAACAGTGC TGCAGACTGA GGGTTCATTC 
CCTGCCCTTG TGAATAGGCT GTTGGTTACC AAGTCTGAAT CCCTTGAATT CTCGCCAGTG 
GAGAATGCGA TTGGAATGAT TGAAACGCGG ACAACCGCTC TGAGAAATGA GCTAGAGGAG 
CCTCGTAGCT CGGATGGGGA TCACCTACCA CGGCTTCAAA GTCTGCAGAG GATTCTCCAA 
GGAAGTGTTG CAGTGCAGGT GAACAGTGGG GTGTTGAGCG TGTGCACAGC TTTCTTATCA 
GGAGAGCCTG CAACGAGATT GCGTTCACAG GAACTGCAGC AACTAATCGC GGCACTGCTT 
GAATTCATGG CGGTTTGTAA GCGAGCCATT AGGGTTCACT TTAGATTAAT CGGAGAAGAA 
GATCAGGAAT TCCATACTCA GCTTGTCAAT GGATTTCAGT CTCTCACCGC AGAACTCTCC 
CATTACATCC CTGCTATCTT ATCCGAACTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_193367.7
CDS	325..5817
Translation	MENNNLGLRFRKLPRQPLALPKLDPLLDENLEQWPHLNQLVQCYGTEWVKDVNKYGHYENIRPDSFQTQIFEGPDTDTETEIRLASARSATIEEDVASISGRPFSDPGSSKHFGQPPLPAYEPAFDWENERAMIFGQRTPESPAASYSSGLKISVRVLSLAFQSGLVEPFFGSIALYNQERKEKLSEDFYFQIQPTEMQDAKLSSENRGVFYLDAPSASVCLLIQLEKTATEEGGVTSSVYSRKEPVHLTEREKQKLQVWSRIMPYRESFAWAVVPLFDNNLTTNTGESASPSSPLAPSMTASSSHDGVYEPIAKITSDGKQGYSGGSSVVVEISNLNKVKESYSEESIQDPKRKVHKPVKGVLRLEIEKHRNGHGDFEDLSENGSIINDSLDPTDRLSDLTLMKCPSSSSGGPRNGCSKWNSEDAKDVSRNLTSSCGTPDLNCYHAFDFCSTTRNEPFLHLFHCLYVYPVAVTLSRKRNPFIRVELRKDDTDIRKQPLEAIYPREPGVSLQKWVHTQVAVGARAASYHDEIKVSLPATWTPSHHLLFTFFHVDLQTKLEAPRPVVVGYASLPLSTYIHSRSDISLPVMRELVPHYLQESTKERLDYLEDGKNIFKLRLRLCSSLYPTNERVRDFCLEYDRHTLQTRPPWGSELLQAINSLKHVDSTALLQFLYPILNMLLHLIGNGGETLQVAAFRAMVDILTRVQQVSFDDADRNRFLVTYVDYSFDDFGGNQPPVYPGLATVWGSLARSKAKGYRVGPVYDDVLSMAWFFLELIVKSMALEQARLYDHNLPTGEDVPPMQLKESVFRCIMQLFDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLYIIEPCQVYELVSLYMDKFSGVCQSVLHECKLTFLQIISDHDLFVEMPGRDPSDRNYLSSILIQELFLSLDHDELPLRAKGARILVILLCKHEFDARYQKAEDKLYIAQLYFPFVGQILDEMPVFYNLNATEKREVLIGVLQIVRNLDDTSLVKAWQQSIARTRLYFKLMEECLILFEHKKAADSILGGNNSRGPVSEGAGSPKYSERLSPAINNYLSEASRQEVRLEGTPDNGYLWQRVNSQLASPSQPYSLREALAQAQSSRIGASAQALRESLHPILRQKLELWEENVSATVSLQVLEITENFSSMAASHNIATDYGKLDCITTILTSFFSRNQSLAFWKAFFPIFNRIFDLHGATLMARENDRFLKQIAFHLLRLAVYRNDSVRKRAVIGLQILVKSSLYFMQTARLRALLTITLSELMSDVQVTHMKSDNTLEESGEARRLQQSLSEMADEAKSVNLLRECGLPDDTLLIIPEKFTENRWSWAEVKHLSDSLVLALDASLGHALLGSVMAMDRYAAAESFYKLGMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAGVIMQALVARNDGVWSKDHVSALRKICPMVSGEFTTEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCASILELVIPVYKSRKAYGQLAKCHTLLTNIYESILDQESNPIPFIDATYYRVGFYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHIYESRMDSNHILHIIPDSRQVKAEDLQAGVCYLQITAVDAVMEDEDLGSRRERIFSLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTTALRNELEEPRSSDGDHLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAVCKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL

Target ORF information:

RefSeq Version	NM_117729.8
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana guanyl-nucleotide exchange factors;GTPase binding;GTP binding protein (SPK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_117729.8

ORF Insert Sequence:

ATGGAGAACA ACAATCTTGG TCTTCGATTT CGTAAGCTAC CTCGTCAGCC TCTTGCTCTC 
CCTAAGCTCG ATCCTTTGCT TGATGAGAAT CTGGAACAAT GGCCACATTT GAATCAATTG 
GTTCAATGTT ATGGCACTGA ATGGGTTAAA GATGTTAACA AATACGGTCA CTATGAAAAT 
ATTCGACCTG ATTCTTTCCA GACTCAGATT TTTGAAGGAC CTGATACTGA TACTGAGACT 
GAGATTCGTC TTGCTAGTGC CAGGAGTGCA ACTATTGAGG AGGATGTTGC CAGTATTTCC 
GGGAGGCCGT TTTCTGATCC TGGATCCTCT AAGCATTTTG GGCAACCTCC TTTACCTGCT 
TATGAACCAG CTTTTGACTG GGAAAATGAA AGAGCTATGA TTTTTGGTCA GAGAACTCCT 
GAATCTCCTG CAGCTAGCTA TTCCAGTGGA TTGAAGATCT CTGTCAGAGT TTTGTCTCTA 
GCTTTTCAGT CTGGCTTAGT TGAACCATTT TTTGGCTCGA TTGCATTGTA CAATCAAGAG 
AGGAAGGAGA AACTCTCTGA GGATTTTTAT TTCCAGATTC AGCCGACGGA AATGCAGGAT 
GCTAAACTTT CATCTGAAAA TCGTGGAGTA TTTTATCTAG ATGCCCCATC AGCATCAGTT 
TGCCTGCTTA TTCAATTAGA AAAAACAGCA ACTGAGGAAG GAGGCGTTAC ATCATCTGTC 
TATTCCCGTA AAGAGCCTGT GCACTTAACT GAGAGGGAAA AGCAAAAGTT GCAGGTCTGG 
TCTCGCATTA TGCCTTACCG AGAGTCTTTT GCTTGGGCAG TTGTTCCGCT TTTTGATAAT 
AATCTCACCA CAAACACTGG TGAATCTGCT TCCCCTAGCA GTCCTCTTGC TCCTAGTATG 
ACTGCGTCAA GTTCCCATGA TGGTGTTTAT GAACCAATTG CAAAGATCAC ATCAGATGGA 
AAGCAAGGTT ATTCAGGTGG AAGTTCTGTC GTAGTTGAAA TATCTAATTT AAATAAAGTT 
AAAGAGAGCT ACTCTGAAGA GTCCATTCAG GACCCCAAAC GGAAGGTTCA CAAACCTGTT 
AAAGGGGTTC TAAGACTGGA AATTGAGAAA CATCGGAATG GACATGGAGA CTTTGAAGAT 
CTATCTGAGA ATGGAAGTAT CATAAATGAC TCCCTTGATC CAACTGACCG CCTCAGTGAC 
CTGACTCTTA TGAAGTGTCC CAGTTCTAGT TCTGGTGGAC CCCGTAATGG CTGTTCAAAG 
TGGAATTCAG AGGATGCAAA GGATGTTTCG AGAAATCTAA CAAGTTCATG TGGGACTCCT 
GACTTAAATT GTTATCATGC TTTTGATTTT TGCTCTACGA CAAGAAACGA GCCTTTTCTA 
CATCTCTTCC ATTGCCTTTA TGTGTATCCT GTAGCTGTTA CTTTAAGTCG GAAGAGGAAT 
CCCTTTATCC GTGTAGAATT GAGAAAGGAT GACACAGATA TCCGAAAACA ACCACTCGAG 
GCAATATATC CAAGAGAACC TGGTGTTTCA CTCCAGAAAT GGGTTCATAC ACAGGTCGCT 
GTTGGTGCTA GGGCCGCTAG CTACCATGAT GAAATTAAGG TCTCTCTTCC TGCCACATGG 
ACGCCATCAC ATCATCTGTT ATTCACTTTT TTTCATGTTG ATCTCCAGAC AAAGCTTGAA 
GCTCCAAGAC CAGTAGTTGT TGGATATGCA TCACTTCCAT TATCTACATA TATCCACTCA 
CGGTCTGATA TTTCTCTACC AGTAATGAGA GAACTTGTTC CCCACTATCT TCAGGAAAGC 
ACCAAGGAGA GGTTGGATTA TTTGGAAGAT GGCAAAAATA TTTTTAAGTT GCGACTGAGA 
CTTTGTTCAT CTCTTTATCC CACCAACGAA CGTGTCAGGG ATTTCTGTCT AGAGTATGAT 
AGACATACCC TCCAGACAAG GCCTCCCTGG GGTTCAGAAC TGTTGCAGGC AATAAACAGT 
TTGAAGCACG TTGATTCTAC TGCCTTGCTT CAGTTTCTTT ACCCAATTCT TAACATGCTC 
CTTCATCTTA TTGGCAATGG TGGAGAAACC CTTCAGGTTG CAGCATTCAG AGCCATGGTG 
GATATTTTAA CTCGGGTGCA GCAGGTGTCG TTTGATGATG CTGACCGAAA TCGATTTCTG 
GTCACCTATG TTGATTATTC TTTTGATGAT TTTGGAGGCA ATCAACCACC GGTTTATCCT 
GGATTAGCAA CTGTGTGGGG AAGTTTAGCT AGAAGCAAGG CCAAAGGTTA CCGGGTTGGA 
CCTGTATATG ACGATGTATT ATCAATGGCG TGGTTTTTCC TCGAGTTGAT TGTTAAATCA 
ATGGCATTGG AGCAAGCACG TCTTTATGAT CACAATCTAC CTACAGGCGA GGATGTTCCA 
CCCATGCAGC TCAAAGAAAG TGTTTTCCGA TGTATCATGC AATTGTTTGA TTGTCTTTTA 
ACTGAAGTAC ATGAACGTTG TAAAAAGGGC TTAAGCTTGG CGAAACGTCT GAACAGCAGT 
TTGGCCTTCT TCTGTTATGA TCTTTTATAT ATCATTGAGC CCTGCCAGGT CTATGAACTG 
GTATCGTTAT ACATGGACAA GTTTTCTGGT GTTTGTCAAT CTGTTCTACA CGAATGCAAG 
CTCACGTTTT TGCAAATTAT CTCCGACCAT GATCTTTTTG TAGAAATGCC TGGTAGAGAC 
CCATCAGACA GGAACTATTT ATCATCCATA TTAATACAGG AGTTATTTCT CTCACTGGAT 
CATGATGAGC TGCCTCTGCG GGCGAAGGGA GCAAGAATTT TAGTGATCCT TTTATGCAAG 
CATGAATTTG ATGCGCGGTA CCAGAAAGCT GAAGATAAGC TGTATATTGC ACAACTGTAT 
TTTCCTTTTG TCGGGCAGAT TTTAGATGAA ATGCCTGTTT TTTATAACCT AAATGCTACT 
GAAAAGCGTG AGGTTTTAAT TGGTGTGCTG CAAATTGTTC GTAATCTGGA TGACACATCA 
CTTGTCAAAG CATGGCAGCA GAGCATCGCT CGTACTCGAT TATATTTCAA ACTCATGGAA 
GAATGCCTCA TACTTTTCGA GCACAAGAAG GCTGCTGACA GCATCCTCGG GGGCAACAAT 
TCTCGTGGTC CTGTTAGTGA AGGAGCTGGA TCACCAAAGT ACTCCGAGCG ACTTTCTCCT 
GCTATCAACA ATTATCTGTC AGAGGCTTCT CGCCAAGAAG TCAGGCTAGA GGGAACACCT 
GATAATGGGT ACCTGTGGCA GAGAGTTAAT TCTCAGTTGG CCTCCCCTAG CCAACCTTAT 
TCTCTAAGAG AAGCTTTAGC TCAAGCACAA TCTTCCCGGA TTGGAGCTTC AGCCCAGGCA 
CTAAGAGAAT CGCTGCACCC AATTTTGAGG CAAAAACTGG AACTTTGGGA GGAGAATGTA 
AGTGCCACTG TTAGCCTCCA GGTTTTGGAG ATCACTGAAA ATTTTTCATC AATGGCAGCC 
TCTCACAATA TTGCGACCGA CTATGGCAAA TTGGACTGCA TTACTACAAT ACTGACGAGT 
TTCTTCTCCC GAAACCAGTC ATTAGCTTTC TGGAAAGCCT TCTTTCCAAT TTTCAACAGA 
ATCTTTGATC TTCATGGGGC TACACTGATG GCCAGGGAAA ATGATCGCTT CTTAAAACAG 
ATAGCCTTCC ATCTTCTCCG GCTTGCTGTT TACCGCAATG ACAGTGTCAG GAAAAGGGCT 
GTTATTGGTC TTCAAATACT AGTTAAGAGC TCGCTATACT TTATGCAGAC TGCTAGGCTG 
AGGGCTTTGC TGACGATTAC ACTGTCAGAA CTCATGTCGG ATGTCCAAGT GACTCATATG 
AAGTCAGATA ATACATTAGA AGAGAGTGGA GAAGCACGGC GACTTCAACA GTCATTAAGC 
GAAATGGCTG ATGAAGCTAA GAGTGTCAAT TTGTTGAGGG AGTGTGGTCT TCCTGATGAT 
ACACTGTTGA TTATTCCCGA GAAATTTACA GAGAACCGCT GGTCATGGGC TGAAGTCAAA 
CATCTTTCTG ACAGCCTTGT TCTTGCTCTT GATGCCAGCC TCGGGCATGC CCTTTTGGGA 
TCTGTGATGG CTATGGATCG ATATGCTGCT GCAGAGAGCT TCTACAAACT TGGAATGGCG 
TTTGCTCCTG TTCCGGATCT TCACATAATG TGGTTATTGC ATCTATGTGA TGCCCACCAG 
GAAATGCAGT CTTGGGCAGA AGCTGCACAG TGTGCTGTGG CGGTTGCAGG TGTGATAATG 
CAGGCCTTGG TGGCTAGGAA TGATGGTGTG TGGAGCAAGG ATCATGTGTC TGCCTTGCGT 
AAGATTTGCC CGATGGTAAG TGGCGAGTTT ACAACAGAGG CATCTGCAGC TGAAGTCGAG 
GGTTATGGTG CTTCAAAGCT AACAGTTGAC TCCGCCGTCA AGTATTTACA GCTTGCTAAT 
AAGCTTTTCT CTCAAGCTGA GCTCTACCAT TTTTGTGCAA GCATTTTAGA ACTTGTTATT 
CCAGTTTACA AGAGCAGGAA GGCTTATGGG CAGTTGGCTA AGTGTCACAC TTTACTTACT 
AATATATACG AGTCCATTCT TGACCAAGAA TCAAACCCAA TCCCATTTAT TGATGCTACA 
TATTACCGGG TGGGATTTTA TGGGGAAAAG TTTGGGAAGT TGGACAGGAA AGAATATGTA 
TACCGAGAAC CACGAGATGT GCGGCTAGGG GATATAATGG AAAAACTGAG CCATATATAT 
GAGTCAAGAA TGGACAGCAA CCATATTCTG CACATTATAC CAGATTCGAG GCAAGTGAAG 
GCAGAAGACT TGCAGGCAGG AGTGTGCTAT CTGCAAATTA CTGCTGTTGA TGCAGTGATG 
GAAGATGAGG ATCTTGGGAG CAGAAGAGAG AGAATATTTT CTCTTTCAAC TGGAAGTGTT 
CGAGCACGAG TGTTTGATCG TTTCTTATTT GACACACCAT TTACGAAAAA TGGTAAAACC 
CAAGGTGGAT TAGAAGATCA ATGGAAGAGA AGAACAGTGC TGCAGACTGA GGGTTCATTC 
CCTGCCCTTG TGAATAGGCT GTTGGTTACC AAGTCTGAAT CCCTTGAATT CTCGCCAGTG 
GAGAATGCGA TTGGAATGAT TGAAACGCGG ACAACCGCTC TGAGAAATGA GCTAGAGGAG 
CCTCGTAGCT CGGATGGGGA TCACCTACCA CGGCTTCAAA GTCTGCAGAG GATTCTCCAA 
GGAAGTGTTG CAGTGCAGGT GAACAGTGGG GTGTTGAGCG TGTGCACAGC TTTCTTATCA 
GGAGAGCCTG CAACGAGATT GCGTTCACAG GAACTGCAGC AACTAATCGC GGCACTGCTT 
GAATTCATGG CGGTTTGTAA GCGAGCCATT AGGGTTCACT TTAGATTAAT CGGAGAAGAA 
GATCAGGAAT TCCATACTCA GCTTGTCAAT GGATTTCAGT CTCTCACCGC AGAACTCTCC 
CATTACATCC CTGCTATCTT ATCCGAACTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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SPK1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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