ATK5 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATK5
Entrez Gene ID	825867
Full Name	kinesin 5
Synonyms	C17L7.110,C17L7_110,kinesin 5
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_116758.4	NP_192428.2	kinesin 5
NM_001340542.1	NP_001329202.1	kinesin 5

Pathways from BioSystems

Homologies

Homo sapiens (human)	KIFC1	NP_002254.2
	KIFC1	XP_001109683.1
Canis lupus familiaris (dog)	KIFC1	XP_005627265.1
Rattus norvegicus (Norway rat)	Kifc1	NP_001005878.1
Gallus gallus (chicken)	LOC101748559	XP_004945725.1
Magnaporthe oryzae (rice blast fungus)	MGG_05350	XP_003710188.1
Oryza sativa (rice)	Os04g0629700	NP_001053970.1
Mus musculus (house mouse)	Kifc1	NP_001182227.1
Mus musculus (house mouse)	Kifc5b	NP_444403.2
Drosophila melanogaster (fruit fly)	ncd	NP_476651.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002248	XP_307936.4
Arabidopsis thaliana (thale cress)	ATK5	NP_192428.2
Arabidopsis thaliana (thale cress)	ATK2	NP_567768.1
Neurospora crassa	NCU04581	XP_958282.1
Arabidopsis thaliana (thale cress)	ATK3	NP_568811.1
Pan troglodytes (chimpanzee)	KIFC1	XP_518406.4
Bos taurus (cattle)	KIFC1	NP_001095406.1
Gallus gallus (chicken)	KIFC1	NP_001075167.1
Danio rerio (zebrafish)	kifc1	NP_571281.1
Arabidopsis thaliana (thale cress)	ATK1	NP_193859.1
Xenopus tropicalis (tropical clawed frog)	kifc1	XP_002938711.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Arabidopsis AtKIN14a and AtKIN14b genes encoding highly similar kinesins are two of the most closely related Arabidopsis paralogs. They together play important roles in controlling plant reproductive development. [AtKIN14b]
Title: Functional divergence of the duplicated AtKIN14a and AtKIN14b genes: critical roles in Arabidopsis meiosis and gametophyte development.

These results are among the first reports of any motor with a major role in anastral spindle structure in plants and demonstrate that the conservation of kinesin-5 motor function throughout eukaryotes extends to vascular plants.
Title: A conserved role for kinesin-5 in plant mitosis.

ATK5 participates in the search and capture of antiparallel interpolar microtubules, where it aids in generating force to coalign microtubules, thereby affecting spindle length, width, and integrity.
Title: The kinesin ATK5 functions in early spindle assembly in Arabidopsis.

ATK5 uses plus-end tracking to reach spindle midzones, where it then organizes microtubules via minus-end-directed motor activity [ATK5]
Title: A minus-end-directed kinesin with plus-end tracking protein activity is involved in spindle morphogenesis.

The following ATK5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATK5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb31861	NM_116758.4 Latest version!	Arabidopsis thaliana kinesin 5 (ATK5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb39037	NM_001340542.1 Latest version!	Arabidopsis thaliana kinesin 5 (ATK5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATK5 ( NM_116758.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_192428.2 Arabidopsis thaliana kinesin 5 (ATK5), mRNA.

Price & Availability↑

CloneID	OAb31861
Clone ID Related Accession (Same CDS sequence)	NM_116758.4
Accession Version	NM_116758.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	kinesin 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_116758.3.

ATGCCACTTC GCAACCAGAA TCGCGCTCCT TTACCTAGTC CAAACGTCAA AAAGGAAGCG 
CTCTCAAGTA TTCCTTTTGA CAAGCGAAGG AAGGAGACGC AAGGTACTGG AAGAAGACAA 
GTGCTTTCGA CAGTGAACAG ACAAGATGCG AACAGCGATG TGGGAAGCAC AGAGGAGTGT 
GGTAAAGTTG AGTTTACAAA AGATGAAGTG TTGGCTTTGC TTAACGAGAG AGCTAAAGCT 
GGAAAATTTG ATACCAAGGG TAAGATTGAG CAAATGACGG ATATTATCAA GAAGCTTAAG 
GTCTGTGTTA GATGGTACCA ACAAGTGGAT GAAACTCATG TTCAAGACAA GGAAAATCTT 
TCTTCTTCTC TTCAATCTGC AGAGAAAAGA TACAGTGATA AAGAGTTGGA TGCAAAGACC 
AAAGAGGAAG AGCTACGAGC AACTATAACA GAGATGAAGG AGAACATAGA ATCACTTCAG 
GAAAAGCTGT CCAAGGAGAA GTTGAGTAAA CTGGATGCAA TTGAAAATCA CAGAAGAGAA 
AAAGATTGTA GAGTTGTAGC TGAGAAGCTT CAGGTTTCTC TCAGAGAAGA GCTTGACAAA 
GTTAAAGAGG AAAAAATGGC TGCGAAGCAG AAGGTGACGT CTCTAGAAGA TATGTACAAG 
AGGCTGCAAG AGTACAACAC AAGTTTGCAA CAATATAACA CCAAACTCCA GACTGATCTT 
GAAGTAGCTA GAGAGGCTCA CACTCGTGCT GAAAAAGAAA AGTCAAGTAT ACTGGAGAAT 
CTTACTACAC TAAGAGGTCA CAGTAAATCA CTACAAGATC AATTAGCATC GAGCCGGGTT 
TCACAAGATG AAGCGGTAAA ACAAAAGGAT TCATTGCTGA TGGAAGTTAA TAACCTTCAA 
AGCGAGCTGC AACAAGTCCG TGATGATCGT GATCGCCATG TTGTACAGTC ACAAAAGCTG 
GCTGGTGAGA TACTGATGTA TAAGGAGAGT GTTGGAAAGT CATCTCATGA GTTGGACATT 
CTTATAGCCA AATCAGGATC TTTAGAGGAA ACATGTTCGT TGCAAAAAGA GCGTATTAAA 
ATGTTGGAGC AGGAATTAGC TTTTGCTAAG GAAAAGCTTA AGATGGTGGA TTTATCTATG 
TCTCATACAA TGACAGAATT TGAGGAGCAG AAACAATGTA TGCATGAACT GCAAGATCGC 
CTTGCAGACA CGGAGCGTCA ACTTTTTGAA GGAGAGTTAT TAAGGAAAAA GCTTCATAAC 
ACCATTCTTG AACTCAAAGG AAACATACGC GTGTTCTGCC GAGTGCGCCC CTTGCTACCA 
GATGATGGAG GACGCCAAGA GGCCAGTGTT ATTGCCTATC CTACATCAAC TGAATCTCTT 
GGTAGAGGCA TTGATGTGGT CCAAAGTGGT AACAAACATC CTTTCACTTT CGACAAGGTC 
TTCGACCACG GAGCTTCTCA GGAGGAAGTA TTCTTTGAAA TATCTCAACT TGTGCAGAGC 
GCATTGGATG GCTACAAGGT TTGTATTTTT GCCTACGGTC AGACAGGTTC TGGCAAAACC 
TATACCATGA TGGGCAGGCC TGAAACACCA GAACAGAAAG GACTGATCCC TCGATCACTC 
GAACAAATAT TCAAAACCAG CCAAAGTCTT AGTACACAAG GCTGGAAATA CAAGATGCAG 
GTCTCAATGT TGGAGATATA TAATGAGTCC ATCAGAGACT TGTTGTCTAC AAGCCGAACG 
ATTGCTATAG AATCTGTGCG GGCAGACAGT AGCACATCTG GAAGACAATA TACAATCACA 
CATGATGTTA ATGGAAACAC TCATGTTAGC GACCTGACAA TAGTAGATGT ATGCAGCATT 
GGACAAATTT CCTCACTTTT GCAACAAGCT GCTCAGAGCA GGTCTGTTGG GAAGACGCAT 
ATGAATGAAC AATCATCAAG AAGTCATTTC GTGTTTACTC TTAGGATTTC TGGTGTGAAT 
GAGAGCACTG AACAGCAAGT ACAAGGTGTT CTTAACCTAA TTGATCTTGC TGGAAGTGAG 
CGACTTTCAA GGAGCGGGGC AACCGGAGAC CGGTTAAAGG AGACACAGGC TATAAACAAA 
AGCTTGTCTG CATTGAGTGA TGTTATATTT GCATTGGCAA AGAAAGAGGA TCATGTACCT 
TTCAGAAACT CAAAGTTAAC ATATCTGCTC CAGCCATGTT TAGGAGGGGA CTCAAAGACC 
TTAATGTTTG TGAACATATC ACCTGATCCA TCTTCAACTG GAGAGTCTCT ATGCTCTCTT 
CGGTTTGCTG CTAGAGTTAA TGCATGTGAG ATTGGAATAC CTCGCAGACA AACTTCCGCA 
AAACTCCTCG ACTCTCGCCT AAGTTACGGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_192428.2
CDS	275..2647
Translation	MPLRNQNRAPLPSPNVKKEALSSIPFDKRRKETQGTGRRQVLSTVNRQDANSDVGSTEECGKVEFTKDEVLALLNERAKAGKFDTKGKIEQMTDIIKKLKVCVRWYQQVDETHVQDKENLSSSLQSAEKRYSDKELDAKTKEEELRATITEMKENIESLQEKLSKEKLSKLDAIENHRREKDCRVVAEKLQVSLREELDKVKEEKMAAKQKVTSLEDMYKRLQEYNTSLQQYNTKLQTDLEVAREAHTRAEKEKSSILENLTTLRGHSKSLQDQLASSRVSQDEAVKQKDSLLMEVNNLQSELQQVRDDRDRHVVQSQKLAGEILMYKESVGKSSHELDILIAKSGSLEETCSLQKERIKMLEQELAFAKEKLKMVDLSMSHTMTEFEEQKQCMHELQDRLADTERQLFEGELLRKKLHNTILELKGNIRVFCRVRPLLPDDGGRQEASVIAYPTSTESLGRGIDVVQSGNKHPFTFDKVFDHGASQEEVFFEISQLVQSALDGYKVCIFAYGQTGSGKTYTMMGRPETPEQKGLIPRSLEQIFKTSQSLSTQGWKYKMQVSMLEIYNESIRDLLSTSRTIAIESVRADSSTSGRQYTITHDVNGNTHVSDLTIVDVCSIGQISSLLQQAAQSRSVGKTHMNEQSSRSHFVFTLRISGVNESTEQQVQGVLNLIDLAGSERLSRSGATGDRLKETQAINKSLSALSDVIFALAKKEDHVPFRNSKLTYLLQPCLGGDSKTLMFVNISPDPSSTGESLCSLRFAARVNACEIGIPRRQTSAKLLDSRLSYG

Target ORF information:

RefSeq Version	NM_116758.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana kinesin 5 (ATK5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_116758.4

ORF Insert Sequence:

ATGCCACTTC GCAACCAGAA TCGCGCTCCT TTACCTAGTC CAAACGTCAA AAAGGAAGCG 
CTCTCAAGTA TTCCTTTTGA CAAGCGAAGG AAGGAGACGC AAGGTACTGG AAGAAGACAA 
GTGCTTTCGA CAGTGAACAG ACAAGATGCG AACAGCGATG TGGGAAGCAC AGAGGAGTGT 
GGTAAAGTTG AGTTTACAAA AGATGAAGTG TTGGCTTTGC TTAACGAGAG AGCTAAAGCT 
GGAAAATTTG ATACCAAGGG TAAGATTGAG CAAATGACGG ATATTATCAA GAAGCTTAAG 
GTCTGTGTTA GATGGTACCA ACAAGTGGAT GAAACTCATG TTCAAGACAA GGAAAATCTT 
TCTTCTTCTC TTCAATCTGC AGAGAAAAGA TACAGTGATA AAGAGTTGGA TGCAAAGACC 
AAAGAGGAAG AGCTACGAGC AACTATAACA GAGATGAAGG AGAACATAGA ATCACTTCAG 
GAAAAGCTGT CCAAGGAGAA GTTGAGTAAA CTGGATGCAA TTGAAAATCA CAGAAGAGAA 
AAAGATTGTA GAGTTGTAGC TGAGAAGCTT CAGGTTTCTC TCAGAGAAGA GCTTGACAAA 
GTTAAAGAGG AAAAAATGGC TGCGAAGCAG AAGGTGACGT CTCTAGAAGA TATGTACAAG 
AGGCTGCAAG AGTACAACAC AAGTTTGCAA CAATATAACA CCAAACTCCA GACTGATCTT 
GAAGTAGCTA GAGAGGCTCA CACTCGTGCT GAAAAAGAAA AGTCAAGTAT ACTGGAGAAT 
CTTACTACAC TAAGAGGTCA CAGTAAATCA CTACAAGATC AATTAGCATC GAGCCGGGTT 
TCACAAGATG AAGCGGTAAA ACAAAAGGAT TCATTGCTGA TGGAAGTTAA TAACCTTCAA 
AGCGAGCTGC AACAAGTCCG TGATGATCGT GATCGCCATG TTGTACAGTC ACAAAAGCTG 
GCTGGTGAGA TACTGATGTA TAAGGAGAGT GTTGGAAAGT CATCTCATGA GTTGGACATT 
CTTATAGCCA AATCAGGATC TTTAGAGGAA ACATGTTCGT TGCAAAAAGA GCGTATTAAA 
ATGTTGGAGC AGGAATTAGC TTTTGCTAAG GAAAAGCTTA AGATGGTGGA TTTATCTATG 
TCTCATACAA TGACAGAATT TGAGGAGCAG AAACAATGTA TGCATGAACT GCAAGATCGC 
CTTGCAGACA CGGAGCGTCA ACTTTTTGAA GGAGAGTTAT TAAGGAAAAA GCTTCATAAC 
ACCATTCTTG AACTCAAAGG AAACATACGC GTGTTCTGCC GAGTGCGCCC CTTGCTACCA 
GATGATGGAG GACGCCAAGA GGCCAGTGTT ATTGCCTATC CTACATCAAC TGAATCTCTT 
GGTAGAGGCA TTGATGTGGT CCAAAGTGGT AACAAACATC CTTTCACTTT CGACAAGGTC 
TTCGACCACG GAGCTTCTCA GGAGGAAGTA TTCTTTGAAA TATCTCAACT TGTGCAGAGC 
GCATTGGATG GCTACAAGGT TTGTATTTTT GCCTACGGTC AGACAGGTTC TGGCAAAACC 
TATACCATGA TGGGCAGGCC TGAAACACCA GAACAGAAAG GACTGATCCC TCGATCACTC 
GAACAAATAT TCAAAACCAG CCAAAGTCTT AGTACACAAG GCTGGAAATA CAAGATGCAG 
GTCTCAATGT TGGAGATATA TAATGAGTCC ATCAGAGACT TGTTGTCTAC AAGCCGAACG 
ATTGCTATAG AATCTGTGCG GGCAGACAGT AGCACATCTG GAAGACAATA TACAATCACA 
CATGATGTTA ATGGAAACAC TCATGTTAGC GACCTGACAA TAGTAGATGT ATGCAGCATT 
GGACAAATTT CCTCACTTTT GCAACAAGCT GCTCAGAGCA GGTCTGTTGG GAAGACGCAT 
ATGAATGAAC AATCATCAAG AAGTCATTTC GTGTTTACTC TTAGGATTTC TGGTGTGAAT 
GAGAGCACTG AACAGCAAGT ACAAGGTGTT CTTAACCTAA TTGATCTTGC TGGAAGTGAG 
CGACTTTCAA GGAGCGGGGC AACCGGAGAC CGGTTAAAGG AGACACAGGC TATAAACAAA 
AGCTTGTCTG CATTGAGTGA TGTTATATTT GCATTGGCAA AGAAAGAGGA TCATGTACCT 
TTCAGAAACT CAAAGTTAAC ATATCTGCTC CAGCCATGTT TAGGAGGGGA CTCAAAGACC 
TTAATGTTTG TGAACATATC ACCTGATCCA TCTTCAACTG GAGAGTCTCT ATGCTCTCTT 
CGGTTTGCTG CTAGAGTTAA TGCATGTGAG ATTGGAATAC CTCGCAGACA AACTTCCGCA 
AAACTCCTCG ACTCTCGCCT AAGTTACGGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATK5 ( NM_001340542.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001329202.1 Arabidopsis thaliana kinesin 5 (ATK5), mRNA.

Price & Availability↑

CloneID	OAb39037
Clone ID Related Accession (Same CDS sequence)	NM_001340542.1
Accession Version	NM_001340542.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	kinesin 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGTTTAAGA AAAAGGAAGC GCTCTCAAGT ATTCCTTTTG ACAAGCGAAG GAAGGAGACG 
CAAGGTACTG GAAGAAGACA AGTGCTTTCG ACAGTGAACA GACAAGATGC GAACAGCGAT 
GTGGGAAGCA CAGAGGAGTG TGGTAAAGTT GAGTTTACAA AAGATGAAGT GTTGGCTTTG 
CTTAACGAGA GAGCTAAAGC TGGAAAATTT GATACCAAGG GTAAGATTGA GCAAATGACG 
GATATTATCA AGAAGCTTAA GGTCTGTGTT AGATGGTACC AACAAGTGGA TGAAACTCAT 
GTTCAAGACA AGGAAAATCT TTCTTCTTCT CTTCAATCTG CAGAGAAAAG ATACAGTGAT 
AAAGAGTTGG ATGCAAAGAC CAAAGAGGAA GAGCTACGAG CAACTATAAC AGAGATGAAG 
GAGAACATAG AATCACTTCA GGAAAAGCTG TCCAAGGAGA AGTTGAGTAA ACTGGATGCA 
ATTGAAAATC ACAGAAGAGA AAAAGATTGT AGAGTTGTAG CTGAGAAGCT TCAGGTTTCT 
CTCAGAGAAG AGCTTGACAA AGTTAAAGAG GAAAAAATGG CTGCGAAGCA GAAGGTGACG 
TCTCTAGAAG ATATGTACAA GAGGCTGCAA GAGTACAACA CAAGTTTGCA ACAATATAAC 
ACCAAACTCC AGACTGATCT TGAAGTAGCT AGAGAGGCTC ACACTCGTGC TGAAAAAGAA 
AAGTCAAGTA TACTGGAGAA TCTTACTACA CTAAGAGGTC ACAGTAAATC ACTACAAGAT 
CAATTAGCAT CGAGCCGGGT TTCACAAGAT GAAGCGGTAA AACAAAAGGA TTCATTGCTG 
ATGGAAGTTA ATAACCTTCA AAGCGAGCTG CAACAAGTCC GTGATGATCG TGATCGCCAT 
GTTGTACAGT CACAAAAGCT GGCTGGTGAG ATACTGATGT ATAAGGAGAG TGTTGGAAAG 
TCATCTCATG AGTTGGACAT TCTTATAGCC AAATCAGGAT CTTTAGAGGA AACATGTTCG 
TTGCAAAAAG AGCGTATTAA AATGTTGGAG CAGGAATTAG CTTTTGCTAA GGAAAAGCTT 
AAGATGGTGG ATTTATCTAT GTCTCATACA ATGACAGAAT TTGAGGAGCA GAAACAATGT 
ATGCATGAAC TGCAAGATCG CCTTGCAGAC ACGGAGCGTC AACTTTTTGA AGGAGAGTTA 
TTAAGGAAAA AGCTTCATAA CACCATTCTT GAACTCAAAG GAAACATACG CGTGTTCTGC 
CGAGTGCGCC CCTTGCTACC AGATGATGGA GGACGCCAAG AGGCCAGTGT TATTGCCTAT 
CCTACATCAA CTGAATCTCT TGGTAGAGGC ATTGATGTGG TCCAAAGTGG TAACAAACAT 
CCTTTCACTT TCGACAAGGT CTTCGACCAC GGAGCTTCTC AGGAGGAAGT ATTCTTTGAA 
ATATCTCAAC TTGTGCAGAG CGCATTGGAT GGCTACAAGG TTTGTATTTT TGCCTACGGT 
CAGACAGGTT CTGGCAAAAC CTATACCATG ATGGGCAGGC CTGAAACACC AGAACAGAAA 
GGACTGATCC CTCGATCACT CGAACAAATA TTCAAAACCA GCCAAAGTCT TAGTACACAA 
GGCTGGAAAT ACAAGATGCA GGTCTCAATG TTGGAGATAT ATAATGAGTC CATCAGAGAC 
TTGTTGTCTA CAAGCCGAAC GATTGCTATA GAATCTGTGC GGGCAGACAG TAGCACATCT 
GGAAGACAAT ATACAATCAC ACATGATGTT AATGGAAACA CTCATGTTAG CGACCTGACA 
ATAGTAGATG TATGCAGCAT TGGACAAATT TCCTCACTTT TGCAACAAGC TGCTCAGAGC 
AGGTCTGTTG GGAAGACGCA TATGAATGAA CAATCATCAA GAAGTCATTT CGTGTTTACT 
CTTAGGATTT CTGGTGTGAA TGAGAGCACT GAACAGCAAG TACAAGGTGT TCTTAACCTA 
ATTGATCTTG CTGGAAGTGA GCGACTTTCA AGGAGCGGGG CAACCGGAGA CCGGTTAAAG 
GAGACACAGG CTATAAACAA AAGCTTGTCT GCATTGAGTG ATGTTATATT TGCATTGGCA 
AAGAAAGAGG ATCATGTACC TTTCAGAAAC TCAAAGTTAA CATATCTGCT CCAGCCATGT 
TTAGGAGGGG ACTCAAAGAC CTTAATGTTT GTGAACATAT CACCTGATCC ATCTTCAACT 
GGAGAGTCTC TATGCTCTCT TCGGTTTGCT GCTAGAGTTA ATGCATGTGA GATTGGAATA 
CCTCGCAGAC AAACTTCCGC AAAACTCCTC GACTCTCGCC TAAGTTACGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001329202.1
CDS	17..2350
Translation	MFKKKEALSSIPFDKRRKETQGTGRRQVLSTVNRQDANSDVGSTEECGKVEFTKDEVLALLNERAKAGKFDTKGKIEQMTDIIKKLKVCVRWYQQVDETHVQDKENLSSSLQSAEKRYSDKELDAKTKEEELRATITEMKENIESLQEKLSKEKLSKLDAIENHRREKDCRVVAEKLQVSLREELDKVKEEKMAAKQKVTSLEDMYKRLQEYNTSLQQYNTKLQTDLEVAREAHTRAEKEKSSILENLTTLRGHSKSLQDQLASSRVSQDEAVKQKDSLLMEVNNLQSELQQVRDDRDRHVVQSQKLAGEILMYKESVGKSSHELDILIAKSGSLEETCSLQKERIKMLEQELAFAKEKLKMVDLSMSHTMTEFEEQKQCMHELQDRLADTERQLFEGELLRKKLHNTILELKGNIRVFCRVRPLLPDDGGRQEASVIAYPTSTESLGRGIDVVQSGNKHPFTFDKVFDHGASQEEVFFEISQLVQSALDGYKVCIFAYGQTGSGKTYTMMGRPETPEQKGLIPRSLEQIFKTSQSLSTQGWKYKMQVSMLEIYNESIRDLLSTSRTIAIESVRADSSTSGRQYTITHDVNGNTHVSDLTIVDVCSIGQISSLLQQAAQSRSVGKTHMNEQSSRSHFVFTLRISGVNESTEQQVQGVLNLIDLAGSERLSRSGATGDRLKETQAINKSLSALSDVIFALAKKEDHVPFRNSKLTYLLQPCLGGDSKTLMFVNISPDPSSTGESLCSLRFAARVNACEIGIPRRQTSAKLLDSRLSYG

Target ORF information:

RefSeq Version	NM_001340542.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana kinesin 5 (ATK5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001340542.1

ORF Insert Sequence:

ATGTTTAAGA AAAAGGAAGC GCTCTCAAGT ATTCCTTTTG ACAAGCGAAG GAAGGAGACG 
CAAGGTACTG GAAGAAGACA AGTGCTTTCG ACAGTGAACA GACAAGATGC GAACAGCGAT 
GTGGGAAGCA CAGAGGAGTG TGGTAAAGTT GAGTTTACAA AAGATGAAGT GTTGGCTTTG 
CTTAACGAGA GAGCTAAAGC TGGAAAATTT GATACCAAGG GTAAGATTGA GCAAATGACG 
GATATTATCA AGAAGCTTAA GGTCTGTGTT AGATGGTACC AACAAGTGGA TGAAACTCAT 
GTTCAAGACA AGGAAAATCT TTCTTCTTCT CTTCAATCTG CAGAGAAAAG ATACAGTGAT 
AAAGAGTTGG ATGCAAAGAC CAAAGAGGAA GAGCTACGAG CAACTATAAC AGAGATGAAG 
GAGAACATAG AATCACTTCA GGAAAAGCTG TCCAAGGAGA AGTTGAGTAA ACTGGATGCA 
ATTGAAAATC ACAGAAGAGA AAAAGATTGT AGAGTTGTAG CTGAGAAGCT TCAGGTTTCT 
CTCAGAGAAG AGCTTGACAA AGTTAAAGAG GAAAAAATGG CTGCGAAGCA GAAGGTGACG 
TCTCTAGAAG ATATGTACAA GAGGCTGCAA GAGTACAACA CAAGTTTGCA ACAATATAAC 
ACCAAACTCC AGACTGATCT TGAAGTAGCT AGAGAGGCTC ACACTCGTGC TGAAAAAGAA 
AAGTCAAGTA TACTGGAGAA TCTTACTACA CTAAGAGGTC ACAGTAAATC ACTACAAGAT 
CAATTAGCAT CGAGCCGGGT TTCACAAGAT GAAGCGGTAA AACAAAAGGA TTCATTGCTG 
ATGGAAGTTA ATAACCTTCA AAGCGAGCTG CAACAAGTCC GTGATGATCG TGATCGCCAT 
GTTGTACAGT CACAAAAGCT GGCTGGTGAG ATACTGATGT ATAAGGAGAG TGTTGGAAAG 
TCATCTCATG AGTTGGACAT TCTTATAGCC AAATCAGGAT CTTTAGAGGA AACATGTTCG 
TTGCAAAAAG AGCGTATTAA AATGTTGGAG CAGGAATTAG CTTTTGCTAA GGAAAAGCTT 
AAGATGGTGG ATTTATCTAT GTCTCATACA ATGACAGAAT TTGAGGAGCA GAAACAATGT 
ATGCATGAAC TGCAAGATCG CCTTGCAGAC ACGGAGCGTC AACTTTTTGA AGGAGAGTTA 
TTAAGGAAAA AGCTTCATAA CACCATTCTT GAACTCAAAG GAAACATACG CGTGTTCTGC 
CGAGTGCGCC CCTTGCTACC AGATGATGGA GGACGCCAAG AGGCCAGTGT TATTGCCTAT 
CCTACATCAA CTGAATCTCT TGGTAGAGGC ATTGATGTGG TCCAAAGTGG TAACAAACAT 
CCTTTCACTT TCGACAAGGT CTTCGACCAC GGAGCTTCTC AGGAGGAAGT ATTCTTTGAA 
ATATCTCAAC TTGTGCAGAG CGCATTGGAT GGCTACAAGG TTTGTATTTT TGCCTACGGT 
CAGACAGGTT CTGGCAAAAC CTATACCATG ATGGGCAGGC CTGAAACACC AGAACAGAAA 
GGACTGATCC CTCGATCACT CGAACAAATA TTCAAAACCA GCCAAAGTCT TAGTACACAA 
GGCTGGAAAT ACAAGATGCA GGTCTCAATG TTGGAGATAT ATAATGAGTC CATCAGAGAC 
TTGTTGTCTA CAAGCCGAAC GATTGCTATA GAATCTGTGC GGGCAGACAG TAGCACATCT 
GGAAGACAAT ATACAATCAC ACATGATGTT AATGGAAACA CTCATGTTAG CGACCTGACA 
ATAGTAGATG TATGCAGCAT TGGACAAATT TCCTCACTTT TGCAACAAGC TGCTCAGAGC 
AGGTCTGTTG GGAAGACGCA TATGAATGAA CAATCATCAA GAAGTCATTT CGTGTTTACT 
CTTAGGATTT CTGGTGTGAA TGAGAGCACT GAACAGCAAG TACAAGGTGT TCTTAACCTA 
ATTGATCTTG CTGGAAGTGA GCGACTTTCA AGGAGCGGGG CAACCGGAGA CCGGTTAAAG 
GAGACACAGG CTATAAACAA AAGCTTGTCT GCATTGAGTG ATGTTATATT TGCATTGGCA 
AAGAAAGAGG ATCATGTACC TTTCAGAAAC TCAAAGTTAA CATATCTGCT CCAGCCATGT 
TTAGGAGGGG ACTCAAAGAC CTTAATGTTT GTGAACATAT CACCTGATCC ATCTTCAACT 
GGAGAGTCTC TATGCTCTCT TCGGTTTGCT GCTAGAGTTA ATGCATGTGA GATTGGAATA 
CCTCGCAGAC AAACTTCCGC AAAACTCCTC GACTCTCGCC TAAGTTACGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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ATK5 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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