ZAR1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZAR1
Entrez Gene ID	824259
Full Name	HOPZ-ACTIVATED RESISTANCE 1
Synonyms	HOPZ-ACTIVATED RESISTANCE 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_114955.4	NP_190664.1	HOPZ-ACTIVATED RESISTANCE 1
NM_180346.1	NP_850677.1	HOPZ-ACTIVATED RESISTANCE 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This detailed analysis of the AtZAR1-AtZED1 protein complex provides a better understanding of the immune signaling hub controlled by AtZAR1.
Title: Analysis of the ZAR1 Immune Complex Reveals Determinants for Immunity and Molecular Interactions.

The disruption of ZAR1 and AGB1 results in short basal cell and an apical cell with basal cell fate
Title: The Arabidopsis Receptor Kinase ZAR1 Is Required for Zygote Asymmetric Division and Its Daughter Cell Fate.

Arabidopsis resistance protein ZAR1 confers allele-specific recognition and virulence attenuation of the Pseudomonas syringae T3SE protein HopZ1a. [ZAR1]
Title: Allele-specific virulence attenuation of the Pseudomonas syringae HopZ1a type III effector via the Arabidopsis ZAR1 resistance protein.

The following ZAR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZAR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb13294	NM_180346.1 Latest version!	Arabidopsis thaliana HOPZ-ACTIVATED RESISTANCE 1 (ZAR1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb13294	NM_114955.4 Latest version!	Arabidopsis thaliana HOPZ-ACTIVATED RESISTANCE 1 (ZAR1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZAR1 ( NM_114955.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_190664.1 Arabidopsis thaliana HOPZ-ACTIVATED RESISTANCE 1 (ZAR1), mRNA.

Price & Availability↑

CloneID	OAb13294
Clone ID Related Accession (Same CDS sequence)	NM_114955.4 , NM_180346.1
Accession Version	NM_114955.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	HOPZ-ACTIVATED RESISTANCE 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Apr 24, 2008 this sequence version replaced NM_114955.3.

ATGGTGGACG CTGTTGTAAC AGTGTTTTTA GAGAAAACCT TGAACATCCT CGAAGAAAAA 
GGCCGAACCG TATCGGACTA CCGAAAACAG TTGGAAGATT TGCAAAGCGA ATTGAAATAT 
ATGCAGTCTT TTCTCAAGGA TGCAGAGAGG CAGAAGAGAA CTAATGAAAC GTTGCGTACT 
CTTGTTGCGG ATTTACGAGA ACTGGTTTAC GAAGCTGAGG ATATACTTGT GGATTGTCAG 
CTCGCGGATG GTGATGATGG GAACGAGCAA AGGTCATCAA ATGCGTGGCT TTCGCGGCTT 
CATCCTGCCC GGGTTCCGTT GCAGTACAAG AAAAGCAAAC GTTTGCAAGA GATCAATGAG 
AGGATAACGA AAATAAAGAG TCAAGTAGAG CCTTATTTCG AATTCATAAC GCCGAGTAAT 
GTTGGAAGAG ATAATGGAAC TGATCGGTGG AGCTCTCCGG TGTATGATCA TACTCAGGTG 
GTTGGTTTGG AAGGAGATAA AAGAAAAATC AAGGAGTGGC TCTTTCGATC AAACGACAGC 
CAGCTTTTGA TAATGGCCTT TGTTGGGATG GGAGGGCTAG GTAAAACTAC AATCGCTCAA 
GAAGTGTTCA ATGACAAAGA GATCGAACAT CGTTTCGAAA GAAGGATATG GGTGTCTGTG 
TCCCAGACTT TTACTGAAGA ACAGATCATG AGAAGCATAT TGAGGAACTT GGGGGACGCG 
AGTGTTGGAG ATGATATTGG AACATTGCTG AGAAAAATCC AGCAGTATCT TTTGGGGAAA 
AGGTACTTGA TAGTTATGGA CGATGTATGG GACAAGAATT TGAGCTGGTG GGACAAGATC 
TACCAAGGAC TGCCTAGAGG ACAAGGAGGT AGCGTTATCG TGACCACAAG GTCGGAATCT 
GTAGCGAAAA GGGTGCAAGC GAGAGACGAC AAGACTCACC GTCCAGAGCT CCTTAGTCCT 
GATAACAGTT GGCTGCTGTT TTGTAATGTG GCATTTGCAG CAAATGACGG TACTTGTGAG 
CGTCCCGAGC TAGAGGATGT TGGCAAGGAG ATTGTAACGA AATGCAAAGG TTTGCCTTTG 
ACAATCAAAG CGGTTGGTGG GTTACTTCTG TGCAAAGACC ATGTCTATCA TGAATGGAGA 
CGAATTGCAG AGCATTTTCA GGATGAGCTA AGAGGAAACA CTTCTGAAAC TGACAATGTT 
ATGTCTTCTC TGCAGTTGAG CTACGATGAA CTCCCTTCCC ACCTTAAGTC GTGCATTCTC 
ACGCTTTCGC TCTATCCAGA AGACTGTGTT ATACCAAAAC AACAACTAGT ACATGGATGG 
ATCGGAGAAG GTTTTGTCAT GTGGAGAAAC GGAAGATCTG CAACTGAATC TGGAGAAGAC 
TGCTTTTCCG GGCTCACAAA CAGGTGTCTA ATAGAAGTTG TAGACAAAAC CTATAGCGGA 
ACCATCATCA CTTGCAAGAT TCATGACATG GTTCGTGATT TGGTGATCGA TATAGCCAAG 
AAGGATTCGT TCTCTAACCC GGAAGGCCTC AACTGTCGTC ACCTTGGGAT TAGTGGAAAC 
TTTGATGAGA AGCAGATCAA AGTTAACCAT AAGCTGAGAG GCGTAGTGTC AACAACTAAG 
ACAGGTGAAG TGAACAAGCT CAACTCAGAC TTAGCTAAGA AATTCACAGA CTGCAAATAT 
CTTCGGGTTC TCGATATCTC GAAATCCATA TTTGATGCTC CTCTGTCTGA GATTTTGGAT 
GAGATTGCAA GCCTTCAACA CTTAGCATGT CTTAGTTTGA GCAACACACA TCCGTTGATC 
CAGTTTCCTC GTTCCATGGA AGATCTCCAC AACCTTCAGA TTCTAGACGC AAGCTACTGT 
CAGAATCTGA AACAACTTCA ACCTTGCATC GTCTTGTTCA AGAAACTCCT TGTACTTGAC 
ATGACGAACT GTGGATCCCT TGAATGCTTC CCCAAGGGTA TCGGAAGTCT CGTGAAGCTC 
GAAGTTCTGT TGGGATTTAA GCCAGCCAGG TCCAACAATG GGTGCAAGCT CAGTGAAGTG 
AAAAATCTCA CAAACCTTCG GAAACTTGGT CTTTCTCTGA CTCGTGGCGA TCAGATAGAA 
GAGGAGGAGC TTGATTCTTT GATAAACCTA AGCAAGCTGA TGTCCATTTC GATCAACTGC 
TATGATAGCT ACGGCGATGA TCTCATAACC AAAATAGACG CCCTTACTCC TCCACACCAG 
CTCCATGAGC TTTCTCTACA ATTCTATCCT GGAAAGTCGA GTCCATCGTG GTTGAGTCCT 
CACAAGCTTC CGATGTTGAG GTACATGTCA ATATGTTCAG GTAATTTGGT GAAAATGCAG 
GAACCATTCT GGGGAAACGA GAATACTCAC TGGAGAATTG AAGGTTTAAT GTTAAGTTCA 
TTGTCGGACC TAGACATGGA CTGGGAAGTG CTGCAACAGT CAATGCCGTA CCTGAGGACC 
GTGACCGCAA ACTGGTGTCC AGAGCTAGAG TCTTTCGCGA TTGAAGATGT TGGCTTTAGA 
GGCGGAGTGT GGATGAAAAC ACCATTGCAC AGAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_190664.1
CDS	226..2784
Translation	MVDAVVTVFLEKTLNILEEKGRTVSDYRKQLEDLQSELKYMQSFLKDAERQKRTNETLRTLVADLRELVYEAEDILVDCQLADGDDGNEQRSSNAWLSRLHPARVPLQYKKSKRLQEINERITKIKSQVEPYFEFITPSNVGRDNGTDRWSSPVYDHTQVVGLEGDKRKIKEWLFRSNDSQLLIMAFVGMGGLGKTTIAQEVFNDKEIEHRFERRIWVSVSQTFTEEQIMRSILRNLGDASVGDDIGTLLRKIQQYLLGKRYLIVMDDVWDKNLSWWDKIYQGLPRGQGGSVIVTTRSESVAKRVQARDDKTHRPELLSPDNSWLLFCNVAFAANDGTCERPELEDVGKEIVTKCKGLPLTIKAVGGLLLCKDHVYHEWRRIAEHFQDELRGNTSETDNVMSSLQLSYDELPSHLKSCILTLSLYPEDCVIPKQQLVHGWIGEGFVMWRNGRSATESGEDCFSGLTNRCLIEVVDKTYSGTIITCKIHDMVRDLVIDIAKKDSFSNPEGLNCRHLGISGNFDEKQIKVNHKLRGVVSTTKTGEVNKLNSDLAKKFTDCKYLRVLDISKSIFDAPLSEILDEIASLQHLACLSLSNTHPLIQFPRSMEDLHNLQILDASYCQNLKQLQPCIVLFKKLLVLDMTNCGSLECFPKGIGSLVKLEVLLGFKPARSNNGCKLSEVKNLTNLRKLGLSLTRGDQIEEEELDSLINLSKLMSISINCYDSYGDDLITKIDALTPPHQLHELSLQFYPGKSSPSWLSPHKLPMLRYMSICSGNLVKMQEPFWGNENTHWRIEGLMLSSLSDLDMDWEVLQQSMPYLRTVTANWCPELESFAIEDVGFRGGVWMKTPLHRT

Target ORF information:

RefSeq Version	NM_114955.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana HOPZ-ACTIVATED RESISTANCE 1 (ZAR1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_114955.4

ORF Insert Sequence:

ATGGTGGACG CTGTTGTAAC AGTGTTTTTA GAGAAAACCT TGAACATCCT CGAAGAAAAA 
GGCCGAACCG TATCGGACTA CCGAAAACAG TTGGAAGATT TGCAAAGCGA ATTGAAATAT 
ATGCAGTCTT TTCTCAAGGA TGCAGAGAGG CAGAAGAGAA CTAATGAAAC GTTGCGTACT 
CTTGTTGCGG ATTTACGAGA ACTGGTTTAC GAAGCTGAGG ATATACTTGT GGATTGTCAG 
CTCGCGGATG GTGATGATGG GAACGAGCAA AGGTCATCAA ATGCGTGGCT TTCGCGGCTT 
CATCCTGCCC GGGTTCCGTT GCAGTACAAG AAAAGCAAAC GTTTGCAAGA GATCAATGAG 
AGGATAACGA AAATAAAGAG TCAAGTAGAG CCTTATTTCG AATTCATAAC GCCGAGTAAT 
GTTGGAAGAG ATAATGGAAC TGATCGGTGG AGCTCTCCGG TGTATGATCA TACTCAGGTG 
GTTGGTTTGG AAGGAGATAA AAGAAAAATC AAGGAGTGGC TCTTTCGATC AAACGACAGC 
CAGCTTTTGA TAATGGCCTT TGTTGGGATG GGAGGGCTAG GTAAAACTAC AATCGCTCAA 
GAAGTGTTCA ATGACAAAGA GATCGAACAT CGTTTCGAAA GAAGGATATG GGTGTCTGTG 
TCCCAGACTT TTACTGAAGA ACAGATCATG AGAAGCATAT TGAGGAACTT GGGGGACGCG 
AGTGTTGGAG ATGATATTGG AACATTGCTG AGAAAAATCC AGCAGTATCT TTTGGGGAAA 
AGGTACTTGA TAGTTATGGA CGATGTATGG GACAAGAATT TGAGCTGGTG GGACAAGATC 
TACCAAGGAC TGCCTAGAGG ACAAGGAGGT AGCGTTATCG TGACCACAAG GTCGGAATCT 
GTAGCGAAAA GGGTGCAAGC GAGAGACGAC AAGACTCACC GTCCAGAGCT CCTTAGTCCT 
GATAACAGTT GGCTGCTGTT TTGTAATGTG GCATTTGCAG CAAATGACGG TACTTGTGAG 
CGTCCCGAGC TAGAGGATGT TGGCAAGGAG ATTGTAACGA AATGCAAAGG TTTGCCTTTG 
ACAATCAAAG CGGTTGGTGG GTTACTTCTG TGCAAAGACC ATGTCTATCA TGAATGGAGA 
CGAATTGCAG AGCATTTTCA GGATGAGCTA AGAGGAAACA CTTCTGAAAC TGACAATGTT 
ATGTCTTCTC TGCAGTTGAG CTACGATGAA CTCCCTTCCC ACCTTAAGTC GTGCATTCTC 
ACGCTTTCGC TCTATCCAGA AGACTGTGTT ATACCAAAAC AACAACTAGT ACATGGATGG 
ATCGGAGAAG GTTTTGTCAT GTGGAGAAAC GGAAGATCTG CAACTGAATC TGGAGAAGAC 
TGCTTTTCCG GGCTCACAAA CAGGTGTCTA ATAGAAGTTG TAGACAAAAC CTATAGCGGA 
ACCATCATCA CTTGCAAGAT TCATGACATG GTTCGTGATT TGGTGATCGA TATAGCCAAG 
AAGGATTCGT TCTCTAACCC GGAAGGCCTC AACTGTCGTC ACCTTGGGAT TAGTGGAAAC 
TTTGATGAGA AGCAGATCAA AGTTAACCAT AAGCTGAGAG GCGTAGTGTC AACAACTAAG 
ACAGGTGAAG TGAACAAGCT CAACTCAGAC TTAGCTAAGA AATTCACAGA CTGCAAATAT 
CTTCGGGTTC TCGATATCTC GAAATCCATA TTTGATGCTC CTCTGTCTGA GATTTTGGAT 
GAGATTGCAA GCCTTCAACA CTTAGCATGT CTTAGTTTGA GCAACACACA TCCGTTGATC 
CAGTTTCCTC GTTCCATGGA AGATCTCCAC AACCTTCAGA TTCTAGACGC AAGCTACTGT 
CAGAATCTGA AACAACTTCA ACCTTGCATC GTCTTGTTCA AGAAACTCCT TGTACTTGAC 
ATGACGAACT GTGGATCCCT TGAATGCTTC CCCAAGGGTA TCGGAAGTCT CGTGAAGCTC 
GAAGTTCTGT TGGGATTTAA GCCAGCCAGG TCCAACAATG GGTGCAAGCT CAGTGAAGTG 
AAAAATCTCA CAAACCTTCG GAAACTTGGT CTTTCTCTGA CTCGTGGCGA TCAGATAGAA 
GAGGAGGAGC TTGATTCTTT GATAAACCTA AGCAAGCTGA TGTCCATTTC GATCAACTGC 
TATGATAGCT ACGGCGATGA TCTCATAACC AAAATAGACG CCCTTACTCC TCCACACCAG 
CTCCATGAGC TTTCTCTACA ATTCTATCCT GGAAAGTCGA GTCCATCGTG GTTGAGTCCT 
CACAAGCTTC CGATGTTGAG GTACATGTCA ATATGTTCAG GTAATTTGGT GAAAATGCAG 
GAACCATTCT GGGGAAACGA GAATACTCAC TGGAGAATTG AAGGTTTAAT GTTAAGTTCA 
TTGTCGGACC TAGACATGGA CTGGGAAGTG CTGCAACAGT CAATGCCGTA CCTGAGGACC 
GTGACCGCAA ACTGGTGTCC AGAGCTAGAG TCTTTCGCGA TTGAAGATGT TGGCTTTAGA 
GGCGGAGTGT GGATGAAAAC ACCATTGCAC AGAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZAR1 ( NM_180346.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_850677.1 Arabidopsis thaliana HOPZ-ACTIVATED RESISTANCE 1 (ZAR1), partial mRNA.

Price & Availability↑

CloneID	OAb13294
Clone ID Related Accession (Same CDS sequence)	NM_114955.4 , NM_180346.1
Accession Version	NM_180346.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	HOPZ-ACTIVATED RESISTANCE 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). COMPLETENESS: incomplete on the 3' end.

ATGGTGGACG CTGTTGTAAC AGTGTTTTTA GAGAAAACCT TGAACATCCT CGAAGAAAAA 
GGCCGAACCG TATCGGACTA CCGAAAACAG TTGGAAGATT TGCAAAGCGA ATTGAAATAT 
ATGCAGTCTT TTCTCAAGGA TGCAGAGAGG CAGAAGAGAA CTAATGAAAC GTTGCGTACT 
CTTGTTGCGG ATTTACGAGA ACTGGTTTAC GAAGCTGAGG ATATACTTGT GGATTGTCAG 
CTCGCGGATG GTGATGATGG GAACGAGCAA AGGTCATCAA ATGCGTGGCT TTCGCGGCTT 
CATCCTGCCC GGGTTCCGTT GCAGTACAAG AAAAGCAAAC GTTTGCAAGA GATCAATGAG 
AGGATAACGA AAATAAAGAG TCAAGTAGAG CCTTATTTCG AATTCATAAC GCCGAGTAAT 
GTTGGAAGAG ATAATGGAAC TGATCGGTGG AGCTCTCCGG TGTATGATCA TACTCAGGTG 
GTTGGTTTGG AAGGAGATAA AAGAAAAATC AAGGAGTGGC TCTTTCGATC AAACGACAGC 
CAGCTTTTGA TAATGGCCTT TGTTGGGATG GGAGGGCTAG GTAAAACTAC AATCGCTCAA 
GAAGTGTTCA ATGACAAAGA GATCGAACAT CGTTTCGAAA GAAGGATATG GGTGTCTGTG 
TCCCAGACTT TTACTGAAGA ACAGATCATG AGAAGCATAT TGAGGAACTT GGGGGACGCG 
AGTGTTGGAG ATGATATTGG AACATTGCTG AGAAAAATCC AGCAGTATCT TTTGGGGAAA 
AGGTACTTGA TAGTTATGGA CGATGTATGG GACAAGAATT TGAGCTGGTG GGACAAGATC 
TACCAAGGAC TGCCTAGAGG ACAAGGAGGT AGCGTTATCG TGACCACAAG GTCGGAATCT 
GTAGCGAAAA GGGTGCAAGC GAGAGACGAC AAGACTCACC GTCCAGAGCT CCTTAGTCCT 
GATAACAGTT GGCTGCTGTT TTGTAATGTG GCATTTGCAG CAAATGACGG TACTTGTGAG 
CGTCCCGAGC TAGAGGATGT TGGCAAGGAG ATTGTAACGA AATGCAAAGG TTTGCCTTTG 
ACAATCAAAG CGGTTGGTGG GTTACTTCTG TGCAAAGACC ATGTCTATCA TGAATGGAGA 
CGAATTGCAG AGCATTTTCA GGATGAGCTA AGAGGAAACA CTTCTGAAAC TGACAATGTT 
ATGTCTTCTC TGCAGTTGAG CTACGATGAA CTCCCTTCCC ACCTTAAGTC GTGCATTCTC 
ACGCTTTCGC TCTATCCAGA AGACTGTGTT ATACCAAAAC AACAACTAGT ACATGGATGG 
ATCGGAGAAG GTTTTGTCAT GTGGAGAAAC GGAAGATCTG CAACTGAATC TGGAGAAGAC 
TGCTTTTCCG GGCTCACAAA CAGGTGTCTA ATAGAAGTTG TAGACAAAAC CTATAGCGGA 
ACCATCATCA CTTGCAAGAT TCATGACATG GTTCGTGATT TGGTGATCGA TATAGCCAAG 
AAGGATTCGT TCTCTAACCC GGAAGGCCTC AACTGTCGTC ACCTTGGGAT TAGTGGAAAC 
TTTGATGAGA AGCAGATCAA AGTTAACCAT AAGCTGAGAG GCGTAGTGTC AACAACTAAG 
ACAGGTGAAG TGAACAAGCT CAACTCAGAC TTAGCTAAGA AATTCACAGA CTGCAAATAT 
CTTCGGGTTC TCGATATCTC GAAATCCATA TTTGATGCTC CTCTGTCTGA GATTTTGGAT 
GAGATTGCAA GCCTTCAACA CTTAGCATGT CTTAGTTTGA GCAACACACA TCCGTTGATC 
CAGTTTCCTC GTTCCATGGA AGATCTCCAC AACCTTCAGA TTCTAGACGC AAGCTACTGT 
CAGAATCTGA AACAACTTCA ACCTTGCATC GTCTTGTTCA AGAAACTCCT TGTACTTGAC 
ATGACGAACT GTGGATCCCT TGAATGCTTC CCCAAGGGTA TCGGAAGTCT CGTGAAGCTC 
GAAGTTCTGT TGGGATTTAA GCCAGCCAGG TCCAACAATG GGTGCAAGCT CAGTGAAGTG 
AAAAATCTCA CAAACCTTCG GAAACTTGGT CTTTCTCTGA CTCGTGGCGA TCAGATAGAA 
GAGGAGGAGC TTGATTCTTT GATAAACCTA AGCAAGCTGA TGTCCATTTC GATCAACTGC 
TATGATAGCT ACGGCGATGA TCTCATAACC AAAATAGACG CCCTTACTCC TCCACACCAG 
CTCCATGAGC TTTCTCTACA ATTCTATCCT GGAAAGTCGA GTCCATCGTG GTTGAGTCCT 
CACAAGCTTC CGATGTTGAG GTACATGTCA ATATGTTCAG GTAATTTGGT GAAAATGCAG 
GAACCATTCT GGGGAAACGA GAATACTCAC TGGAGAATTG AAGGTTTAAT GTTAAGTTCA 
TTGTCGGACC TAGACATGGA CTGGGAAGTG CTGCAACAGT CAATGCCGTA CCTGAGGACC 
GTGACCGCAA ACTGGTGTCC AGAGCTAGAG TCTTTCGCGA TTGAAGATGT TGGCTTTAGA 
GGCGGAGTGT GGATGAAAAC ACCATTGCAC AGAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_850677.1
CDS	192..2750
Translation	MVDAVVTVFLEKTLNILEEKGRTVSDYRKQLEDLQSELKYMQSFLKDAERQKRTNETLRTLVADLRELVYEAEDILVDCQLADGDDGNEQRSSNAWLSRLHPARVPLQYKKSKRLQEINERITKIKSQVEPYFEFITPSNVGRDNGTDRWSSPVYDHTQVVGLEGDKRKIKEWLFRSNDSQLLIMAFVGMGGLGKTTIAQEVFNDKEIEHRFERRIWVSVSQTFTEEQIMRSILRNLGDASVGDDIGTLLRKIQQYLLGKRYLIVMDDVWDKNLSWWDKIYQGLPRGQGGSVIVTTRSESVAKRVQARDDKTHRPELLSPDNSWLLFCNVAFAANDGTCERPELEDVGKEIVTKCKGLPLTIKAVGGLLLCKDHVYHEWRRIAEHFQDELRGNTSETDNVMSSLQLSYDELPSHLKSCILTLSLYPEDCVIPKQQLVHGWIGEGFVMWRNGRSATESGEDCFSGLTNRCLIEVVDKTYSGTIITCKIHDMVRDLVIDIAKKDSFSNPEGLNCRHLGISGNFDEKQIKVNHKLRGVVSTTKTGEVNKLNSDLAKKFTDCKYLRVLDISKSIFDAPLSEILDEIASLQHLACLSLSNTHPLIQFPRSMEDLHNLQILDASYCQNLKQLQPCIVLFKKLLVLDMTNCGSLECFPKGIGSLVKLEVLLGFKPARSNNGCKLSEVKNLTNLRKLGLSLTRGDQIEEEELDSLINLSKLMSISINCYDSYGDDLITKIDALTPPHQLHELSLQFYPGKSSPSWLSPHKLPMLRYMSICSGNLVKMQEPFWGNENTHWRIEGLMLSSLSDLDMDWEVLQQSMPYLRTVTANWCPELESFAIEDVGFRGGVWMKTPLHRT

Target ORF information:

RefSeq Version	NM_180346.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana HOPZ-ACTIVATED RESISTANCE 1 (ZAR1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_180346.1

ORF Insert Sequence:

ATGGTGGACG CTGTTGTAAC AGTGTTTTTA GAGAAAACCT TGAACATCCT CGAAGAAAAA 
GGCCGAACCG TATCGGACTA CCGAAAACAG TTGGAAGATT TGCAAAGCGA ATTGAAATAT 
ATGCAGTCTT TTCTCAAGGA TGCAGAGAGG CAGAAGAGAA CTAATGAAAC GTTGCGTACT 
CTTGTTGCGG ATTTACGAGA ACTGGTTTAC GAAGCTGAGG ATATACTTGT GGATTGTCAG 
CTCGCGGATG GTGATGATGG GAACGAGCAA AGGTCATCAA ATGCGTGGCT TTCGCGGCTT 
CATCCTGCCC GGGTTCCGTT GCAGTACAAG AAAAGCAAAC GTTTGCAAGA GATCAATGAG 
AGGATAACGA AAATAAAGAG TCAAGTAGAG CCTTATTTCG AATTCATAAC GCCGAGTAAT 
GTTGGAAGAG ATAATGGAAC TGATCGGTGG AGCTCTCCGG TGTATGATCA TACTCAGGTG 
GTTGGTTTGG AAGGAGATAA AAGAAAAATC AAGGAGTGGC TCTTTCGATC AAACGACAGC 
CAGCTTTTGA TAATGGCCTT TGTTGGGATG GGAGGGCTAG GTAAAACTAC AATCGCTCAA 
GAAGTGTTCA ATGACAAAGA GATCGAACAT CGTTTCGAAA GAAGGATATG GGTGTCTGTG 
TCCCAGACTT TTACTGAAGA ACAGATCATG AGAAGCATAT TGAGGAACTT GGGGGACGCG 
AGTGTTGGAG ATGATATTGG AACATTGCTG AGAAAAATCC AGCAGTATCT TTTGGGGAAA 
AGGTACTTGA TAGTTATGGA CGATGTATGG GACAAGAATT TGAGCTGGTG GGACAAGATC 
TACCAAGGAC TGCCTAGAGG ACAAGGAGGT AGCGTTATCG TGACCACAAG GTCGGAATCT 
GTAGCGAAAA GGGTGCAAGC GAGAGACGAC AAGACTCACC GTCCAGAGCT CCTTAGTCCT 
GATAACAGTT GGCTGCTGTT TTGTAATGTG GCATTTGCAG CAAATGACGG TACTTGTGAG 
CGTCCCGAGC TAGAGGATGT TGGCAAGGAG ATTGTAACGA AATGCAAAGG TTTGCCTTTG 
ACAATCAAAG CGGTTGGTGG GTTACTTCTG TGCAAAGACC ATGTCTATCA TGAATGGAGA 
CGAATTGCAG AGCATTTTCA GGATGAGCTA AGAGGAAACA CTTCTGAAAC TGACAATGTT 
ATGTCTTCTC TGCAGTTGAG CTACGATGAA CTCCCTTCCC ACCTTAAGTC GTGCATTCTC 
ACGCTTTCGC TCTATCCAGA AGACTGTGTT ATACCAAAAC AACAACTAGT ACATGGATGG 
ATCGGAGAAG GTTTTGTCAT GTGGAGAAAC GGAAGATCTG CAACTGAATC TGGAGAAGAC 
TGCTTTTCCG GGCTCACAAA CAGGTGTCTA ATAGAAGTTG TAGACAAAAC CTATAGCGGA 
ACCATCATCA CTTGCAAGAT TCATGACATG GTTCGTGATT TGGTGATCGA TATAGCCAAG 
AAGGATTCGT TCTCTAACCC GGAAGGCCTC AACTGTCGTC ACCTTGGGAT TAGTGGAAAC 
TTTGATGAGA AGCAGATCAA AGTTAACCAT AAGCTGAGAG GCGTAGTGTC AACAACTAAG 
ACAGGTGAAG TGAACAAGCT CAACTCAGAC TTAGCTAAGA AATTCACAGA CTGCAAATAT 
CTTCGGGTTC TCGATATCTC GAAATCCATA TTTGATGCTC CTCTGTCTGA GATTTTGGAT 
GAGATTGCAA GCCTTCAACA CTTAGCATGT CTTAGTTTGA GCAACACACA TCCGTTGATC 
CAGTTTCCTC GTTCCATGGA AGATCTCCAC AACCTTCAGA TTCTAGACGC AAGCTACTGT 
CAGAATCTGA AACAACTTCA ACCTTGCATC GTCTTGTTCA AGAAACTCCT TGTACTTGAC 
ATGACGAACT GTGGATCCCT TGAATGCTTC CCCAAGGGTA TCGGAAGTCT CGTGAAGCTC 
GAAGTTCTGT TGGGATTTAA GCCAGCCAGG TCCAACAATG GGTGCAAGCT CAGTGAAGTG 
AAAAATCTCA CAAACCTTCG GAAACTTGGT CTTTCTCTGA CTCGTGGCGA TCAGATAGAA 
GAGGAGGAGC TTGATTCTTT GATAAACCTA AGCAAGCTGA TGTCCATTTC GATCAACTGC 
TATGATAGCT ACGGCGATGA TCTCATAACC AAAATAGACG CCCTTACTCC TCCACACCAG 
CTCCATGAGC TTTCTCTACA ATTCTATCCT GGAAAGTCGA GTCCATCGTG GTTGAGTCCT 
CACAAGCTTC CGATGTTGAG GTACATGTCA ATATGTTCAG GTAATTTGGT GAAAATGCAG 
GAACCATTCT GGGGAAACGA GAATACTCAC TGGAGAATTG AAGGTTTAAT GTTAAGTTCA 
TTGTCGGACC TAGACATGGA CTGGGAAGTG CTGCAACAGT CAATGCCGTA CCTGAGGACC 
GTGACCGCAA ACTGGTGTCC AGAGCTAGAG TCTTTCGCGA TTGAAGATGT TGGCTTTAGA 
GGCGGAGTGT GGATGAAAAC ACCATTGCAC AGAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Nature408(6814)820-2(2000 Dec)
Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,

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ZAR1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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