DWF1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	DWF1
Entrez Gene ID	821519
Full Name	cell elongation protein / DWARF1 / DIMINUTO (DIM)
Synonyms	CABBAGE 1,CBB1,DIM,DIM1,DIMINUTIA,DIMINUTO 1,DWARF 1,ENHANCED VERY-LOW-FLUENCE RESPONSES 1,EVE1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_180285.4	NP_850616.1	cell elongation protein / DWARF1 / DIMINUTO (DIM)
NM_001338420.1	NP_001319595.1	cell elongation protein / DWARF1 / DIMINUTO (DIM)
NM_112872.3	NP_188616.1	cell elongation protein / DWARF1 / DIMINUTO (DIM)

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DHCR24	XP_546693.4
Mus musculus (house mouse)	Dhcr24	NP_444502.2
Gallus gallus (chicken)	DHCR24	NP_001026459.1
Magnaporthe oryzae (rice blast fungus)	MGG_16834	XP_003712445.1
Homo sapiens (human)	DHCR24	NP_055577.1
Xenopus tropicalis (tropical clawed frog)	dhcr24	NP_001016800.1
	DHCR24	XP_001108186.2
Rattus norvegicus (Norway rat)	Dhcr24	NP_001073617.1
Arabidopsis thaliana (thale cress)	DWF1	NP_850616.1
Pan troglodytes (chimpanzee)	DHCR24	XP_001153930.1
Bos taurus (cattle)	DHCR24	NP_001096746.1
Danio rerio (zebrafish)	dhcr24	NP_001008645.1
Caenorhabditis elegans (roundworm)	F52H2.6	NP_508463.1
Oryza sativa (rice)	Os10g0397400	NP_001064534.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

both Delta(5,7)-sterol-Delta(7)-reductase and Delta(24)-sterol-Delta(24)-reductase are in addition localized to the plasma membrane, whereas Delta(7)-sterol-C(5)-desaturase was clearly detected in lipid particles
Title: Plant sterol metabolism. ?(7)-Sterol-C5-desaturase (STE1/DWARF7), ?(5,7)-sterol-?(7)-reductase (DWARF5) and ?(24)-sterol-?(24)-reductase (DIMINUTO/DWARF1) show multiple subcellular localizations in Arabidopsis thaliana (Heynh) L.

Data indicate a role for DIM1 in secondary cell wall formation.
Title: DIMINUTO 1 affects the lignin profile and secondary cell wall formation in Arabidopsis.

The genomic location of the EVE1 gene in Arabidopsis thaliana corresponded to a bacterial artificial chromosome (BAC) F4C21 from chromosome IV at ~17cM which encoded a novel ubiquitin family protein (At4g03350), consisting of a single exon.
Title: Overexpression of EVE1, a novel ubiquitin family protein, arrests inflorescence stem development in Arabidopsis.

results provide direct proof that Ca2+/calmodulin-mediated signalling has a critical role in controlling the function of DWF1 [DWARF1]
Title: Ca2+/calmodulin is critical for brassinosteroid biosynthesis and plant growth.

Overproduction and repression of Dim/dwf1 resulted in contrasting phenotypes, with repressors mimicking the brd2 phenotype and overproducers having large stature with increased numbers of flowers and seeds.
Title: The Rice brassinosteroid-deficient dwarf2 mutant, defective in the rice homolog of Arabidopsis DIMINUTO/DWARF1, is rescued by the endogenously accumulated alternative bioactive brassinosteroid, dolichosterone.

The following DWF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DWF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb11868	NM_112872.3 Latest version!	Arabidopsis thaliana cell elongation protein / DWARF1 / DIMINUTO (DIM) (DWF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb11868	NM_001338420.1 Latest version!	Arabidopsis thaliana cell elongation protein / DWARF1 / DIMINUTO (DIM) (DWF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb11868	NM_180285.4 Latest version!	Arabidopsis thaliana cell elongation protein / DWARF1 / DIMINUTO (DIM) (DWF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DWF1 ( NM_180285.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_850616.1 Arabidopsis thaliana cell elongation protein / DWARF1 / DIMINUTO (DIM) (DWF1), mRNA.

Price & Availability↑

CloneID	OAb11868
Clone ID Related Accession (Same CDS sequence)	NM_180285.4 , NM_001338420.1 , NM_112872.3
Accession Version	NM_180285.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	cell elongation protein / DWARF1 / DIMINUTO (DIM)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_180285.3.

ATGTCGGATC TTCAGACACC GCTTGTGAGG CCCAAGAGGA AGAAGACTTG GGTTGATTAC 
TTTGTCAAGT TCAGATGGAT CATTGTCATC TTCATCGTCC TTCCATTCTC AGCCACATTC 
TACTTCCTCA TCTACCTCGG GGACATGTGG TCAGAGTCCA AGTCCTTTGA GAAACGTCAG 
AAGGAACACG ACGAGAATGT CAAGAAAGTC ATCAAAAGGC TTAAGGGTAG GGATGCTTCC 
AAGGACGGGC TTGTCTGCAC TGCTCGTAAG CCCTGGATCG CTGTTGGAAT GAGGAACGTT 
GACTACAAGA GAGCCCGGCA TTTCGAGGTT GACTTGGGGG AGTTCCGTAA CATCCTTGAG 
ATCAACAAGG AGAAGATGAC TGCTAGAGTG GAGCCTCTTG TTAACATGGG ACAGATTTCC 
CGTGCTACCG TCCCAATGAA CCTGTCTCTC GCTGTTGTTG CTGAGCTTGA TGACCTTACC 
GTTGGTGGAC TTATCAATGG ATATGGTATT GAAGGAAGCT CTCACATCTA CGGTTTGTTT 
GCTGATACCG TTGAGGCTTA CGAGATTGTT CTTGCGGGTG GAGAGCTTGT CCGCGCCACA 
AGGGATAATG AGTATTCTGA TCTTTACTAC GCAATCCCGT GGTCGCAAGG AACTCTTGGA 
CTCCTTGTAG CTGCTGAGAT CAGGCTTATT AAAGTCAAGG AGTACATGAG ACTCACTTAC 
ATACCAGTCA AGGGTGATCT TCAAGCCTTA GCTCAAGGTT ACATTGATTC TTTTGCTCCC 
AAAGACGGTG ACAAGTCGAA AATCCCGGAT TTCGTCGAAG GCATGGTTTA CAATCCAACG 
GAAGGAGTGA TGATGGTTGG AACATATGCA TCTAAAGAAG AGGCAAAGAA GAAAGGGAAC 
AAAATCAACA ATGTGGGATG GTGGTTCAAG CCGTGGTTCT ACCAGCACGC GCAGACCGCC 
CTGAAAAAGG GACAGTTTGT TGAGTACATC CCAACTCGTG AATACTACCA CAGGCACACA 
AGGTGCTTGT ACTGGGAAGG GAAGCTTATT CTTCCATTTG GTGATCAGTT CTGGTTTAGG 
TACCTCTTAG GTTGGTTGAT GCCTCCAAAG GTCTCTCTTC TTAAGGCCAC TCAAGGTGAA 
GCTATCAGGA ACTATTACCA TGATATGCAT GTTATTCAGG ATATGCTTGT TCCTCTTTAC 
AAGGTTGGCG ATGCACTCGA ATGGGTCCAC CGCGAAATGG AGGTGTATCC AATTTGGCTT 
TGCCCACACA AACTCTTCAA GCAGCCAATC AAAGGCCAAA TCTACCCAGA GCCAGGCTTC 
GAGTACGAAA ACAGACAAGG AGACACAGAA GATGCACAGA TGTACACTGA TGTTGGAGTC 
TACTACGCAC CTGGCTGTGT CCTAAGAGGT GAAGAGTTTG ATGGATCAGA AGCAGTGCGT 
AGGATGGAGA AATGGCTGAT AGAGAACCAT GGATTCCAGC CTCAGTACGC GGTGTCTGAG 
CTCGACGAGA AGAGCTTCTG GAGAATGTTT AATGGTGAAT TGTATGAGGA GTGCCGCAAG 
AAGTATAGAG CTATTGGAAC GTTCATGAGT GTTTACTACA AGTCCAAGAA AGGAAGGAAG 
ACTGAGAAAG AAGTTAGAGA AGCCGAACAA GCTCATCTCG AAACTGCTTA TGCCGAGGCA 
GATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_850616.1
CDS	117..1802
Translation	MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQKEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILEINKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTYIPVKGDLQALAQGYIDSFAPKDGDKSKIPDFVEGMVYNPTEGVMMVGTYASKEEAKKKGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQFWFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYPIWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSEAVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKKGRKTEKEVREAEQAHLETAYAEAD

Target ORF information:

RefSeq Version	NM_180285.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana cell elongation protein / DWARF1 / DIMINUTO (DIM) (DWF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_180285.4

ORF Insert Sequence:

ATGTCGGATC TTCAGACACC GCTTGTGAGG CCCAAGAGGA AGAAGACTTG GGTTGATTAC 
TTTGTCAAGT TCAGATGGAT CATTGTCATC TTCATCGTCC TTCCATTCTC AGCCACATTC 
TACTTCCTCA TCTACCTCGG GGACATGTGG TCAGAGTCCA AGTCCTTTGA GAAACGTCAG 
AAGGAACACG ACGAGAATGT CAAGAAAGTC ATCAAAAGGC TTAAGGGTAG GGATGCTTCC 
AAGGACGGGC TTGTCTGCAC TGCTCGTAAG CCCTGGATCG CTGTTGGAAT GAGGAACGTT 
GACTACAAGA GAGCCCGGCA TTTCGAGGTT GACTTGGGGG AGTTCCGTAA CATCCTTGAG 
ATCAACAAGG AGAAGATGAC TGCTAGAGTG GAGCCTCTTG TTAACATGGG ACAGATTTCC 
CGTGCTACCG TCCCAATGAA CCTGTCTCTC GCTGTTGTTG CTGAGCTTGA TGACCTTACC 
GTTGGTGGAC TTATCAATGG ATATGGTATT GAAGGAAGCT CTCACATCTA CGGTTTGTTT 
GCTGATACCG TTGAGGCTTA CGAGATTGTT CTTGCGGGTG GAGAGCTTGT CCGCGCCACA 
AGGGATAATG AGTATTCTGA TCTTTACTAC GCAATCCCGT GGTCGCAAGG AACTCTTGGA 
CTCCTTGTAG CTGCTGAGAT CAGGCTTATT AAAGTCAAGG AGTACATGAG ACTCACTTAC 
ATACCAGTCA AGGGTGATCT TCAAGCCTTA GCTCAAGGTT ACATTGATTC TTTTGCTCCC 
AAAGACGGTG ACAAGTCGAA AATCCCGGAT TTCGTCGAAG GCATGGTTTA CAATCCAACG 
GAAGGAGTGA TGATGGTTGG AACATATGCA TCTAAAGAAG AGGCAAAGAA GAAAGGGAAC 
AAAATCAACA ATGTGGGATG GTGGTTCAAG CCGTGGTTCT ACCAGCACGC GCAGACCGCC 
CTGAAAAAGG GACAGTTTGT TGAGTACATC CCAACTCGTG AATACTACCA CAGGCACACA 
AGGTGCTTGT ACTGGGAAGG GAAGCTTATT CTTCCATTTG GTGATCAGTT CTGGTTTAGG 
TACCTCTTAG GTTGGTTGAT GCCTCCAAAG GTCTCTCTTC TTAAGGCCAC TCAAGGTGAA 
GCTATCAGGA ACTATTACCA TGATATGCAT GTTATTCAGG ATATGCTTGT TCCTCTTTAC 
AAGGTTGGCG ATGCACTCGA ATGGGTCCAC CGCGAAATGG AGGTGTATCC AATTTGGCTT 
TGCCCACACA AACTCTTCAA GCAGCCAATC AAAGGCCAAA TCTACCCAGA GCCAGGCTTC 
GAGTACGAAA ACAGACAAGG AGACACAGAA GATGCACAGA TGTACACTGA TGTTGGAGTC 
TACTACGCAC CTGGCTGTGT CCTAAGAGGT GAAGAGTTTG ATGGATCAGA AGCAGTGCGT 
AGGATGGAGA AATGGCTGAT AGAGAACCAT GGATTCCAGC CTCAGTACGC GGTGTCTGAG 
CTCGACGAGA AGAGCTTCTG GAGAATGTTT AATGGTGAAT TGTATGAGGA GTGCCGCAAG 
AAGTATAGAG CTATTGGAAC GTTCATGAGT GTTTACTACA AGTCCAAGAA AGGAAGGAAG 
ACTGAGAAAG AAGTTAGAGA AGCCGAACAA GCTCATCTCG AAACTGCTTA TGCCGAGGCA 
GATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DWF1 ( NM_001338420.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001319595.1 Arabidopsis thaliana cell elongation protein / DWARF1 / DIMINUTO (DIM) (DWF1), mRNA.

Price & Availability↑

CloneID	OAb11868
Clone ID Related Accession (Same CDS sequence)	NM_180285.4 , NM_001338420.1 , NM_112872.3
Accession Version	NM_001338420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	cell elongation protein / DWARF1 / DIMINUTO (DIM)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074).

ATGTCGGATC TTCAGACACC GCTTGTGAGG CCCAAGAGGA AGAAGACTTG GGTTGATTAC 
TTTGTCAAGT TCAGATGGAT CATTGTCATC TTCATCGTCC TTCCATTCTC AGCCACATTC 
TACTTCCTCA TCTACCTCGG GGACATGTGG TCAGAGTCCA AGTCCTTTGA GAAACGTCAG 
AAGGAACACG ACGAGAATGT CAAGAAAGTC ATCAAAAGGC TTAAGGGTAG GGATGCTTCC 
AAGGACGGGC TTGTCTGCAC TGCTCGTAAG CCCTGGATCG CTGTTGGAAT GAGGAACGTT 
GACTACAAGA GAGCCCGGCA TTTCGAGGTT GACTTGGGGG AGTTCCGTAA CATCCTTGAG 
ATCAACAAGG AGAAGATGAC TGCTAGAGTG GAGCCTCTTG TTAACATGGG ACAGATTTCC 
CGTGCTACCG TCCCAATGAA CCTGTCTCTC GCTGTTGTTG CTGAGCTTGA TGACCTTACC 
GTTGGTGGAC TTATCAATGG ATATGGTATT GAAGGAAGCT CTCACATCTA CGGTTTGTTT 
GCTGATACCG TTGAGGCTTA CGAGATTGTT CTTGCGGGTG GAGAGCTTGT CCGCGCCACA 
AGGGATAATG AGTATTCTGA TCTTTACTAC GCAATCCCGT GGTCGCAAGG AACTCTTGGA 
CTCCTTGTAG CTGCTGAGAT CAGGCTTATT AAAGTCAAGG AGTACATGAG ACTCACTTAC 
ATACCAGTCA AGGGTGATCT TCAAGCCTTA GCTCAAGGTT ACATTGATTC TTTTGCTCCC 
AAAGACGGTG ACAAGTCGAA AATCCCGGAT TTCGTCGAAG GCATGGTTTA CAATCCAACG 
GAAGGAGTGA TGATGGTTGG AACATATGCA TCTAAAGAAG AGGCAAAGAA GAAAGGGAAC 
AAAATCAACA ATGTGGGATG GTGGTTCAAG CCGTGGTTCT ACCAGCACGC GCAGACCGCC 
CTGAAAAAGG GACAGTTTGT TGAGTACATC CCAACTCGTG AATACTACCA CAGGCACACA 
AGGTGCTTGT ACTGGGAAGG GAAGCTTATT CTTCCATTTG GTGATCAGTT CTGGTTTAGG 
TACCTCTTAG GTTGGTTGAT GCCTCCAAAG GTCTCTCTTC TTAAGGCCAC TCAAGGTGAA 
GCTATCAGGA ACTATTACCA TGATATGCAT GTTATTCAGG ATATGCTTGT TCCTCTTTAC 
AAGGTTGGCG ATGCACTCGA ATGGGTCCAC CGCGAAATGG AGGTGTATCC AATTTGGCTT 
TGCCCACACA AACTCTTCAA GCAGCCAATC AAAGGCCAAA TCTACCCAGA GCCAGGCTTC 
GAGTACGAAA ACAGACAAGG AGACACAGAA GATGCACAGA TGTACACTGA TGTTGGAGTC 
TACTACGCAC CTGGCTGTGT CCTAAGAGGT GAAGAGTTTG ATGGATCAGA AGCAGTGCGT 
AGGATGGAGA AATGGCTGAT AGAGAACCAT GGATTCCAGC CTCAGTACGC GGTGTCTGAG 
CTCGACGAGA AGAGCTTCTG GAGAATGTTT AATGGTGAAT TGTATGAGGA GTGCCGCAAG 
AAGTATAGAG CTATTGGAAC GTTCATGAGT GTTTACTACA AGTCCAAGAA AGGAAGGAAG 
ACTGAGAAAG AAGTTAGAGA AGCCGAACAA GCTCATCTCG AAACTGCTTA TGCCGAGGCA 
GATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001319595.1
CDS	526..2211
Translation	MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQKEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILEINKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTYIPVKGDLQALAQGYIDSFAPKDGDKSKIPDFVEGMVYNPTEGVMMVGTYASKEEAKKKGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQFWFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYPIWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSEAVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKKGRKTEKEVREAEQAHLETAYAEAD

Target ORF information:

RefSeq Version	NM_001338420.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana cell elongation protein / DWARF1 / DIMINUTO (DIM) (DWF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001338420.1

ORF Insert Sequence:

ATGTCGGATC TTCAGACACC GCTTGTGAGG CCCAAGAGGA AGAAGACTTG GGTTGATTAC 
TTTGTCAAGT TCAGATGGAT CATTGTCATC TTCATCGTCC TTCCATTCTC AGCCACATTC 
TACTTCCTCA TCTACCTCGG GGACATGTGG TCAGAGTCCA AGTCCTTTGA GAAACGTCAG 
AAGGAACACG ACGAGAATGT CAAGAAAGTC ATCAAAAGGC TTAAGGGTAG GGATGCTTCC 
AAGGACGGGC TTGTCTGCAC TGCTCGTAAG CCCTGGATCG CTGTTGGAAT GAGGAACGTT 
GACTACAAGA GAGCCCGGCA TTTCGAGGTT GACTTGGGGG AGTTCCGTAA CATCCTTGAG 
ATCAACAAGG AGAAGATGAC TGCTAGAGTG GAGCCTCTTG TTAACATGGG ACAGATTTCC 
CGTGCTACCG TCCCAATGAA CCTGTCTCTC GCTGTTGTTG CTGAGCTTGA TGACCTTACC 
GTTGGTGGAC TTATCAATGG ATATGGTATT GAAGGAAGCT CTCACATCTA CGGTTTGTTT 
GCTGATACCG TTGAGGCTTA CGAGATTGTT CTTGCGGGTG GAGAGCTTGT CCGCGCCACA 
AGGGATAATG AGTATTCTGA TCTTTACTAC GCAATCCCGT GGTCGCAAGG AACTCTTGGA 
CTCCTTGTAG CTGCTGAGAT CAGGCTTATT AAAGTCAAGG AGTACATGAG ACTCACTTAC 
ATACCAGTCA AGGGTGATCT TCAAGCCTTA GCTCAAGGTT ACATTGATTC TTTTGCTCCC 
AAAGACGGTG ACAAGTCGAA AATCCCGGAT TTCGTCGAAG GCATGGTTTA CAATCCAACG 
GAAGGAGTGA TGATGGTTGG AACATATGCA TCTAAAGAAG AGGCAAAGAA GAAAGGGAAC 
AAAATCAACA ATGTGGGATG GTGGTTCAAG CCGTGGTTCT ACCAGCACGC GCAGACCGCC 
CTGAAAAAGG GACAGTTTGT TGAGTACATC CCAACTCGTG AATACTACCA CAGGCACACA 
AGGTGCTTGT ACTGGGAAGG GAAGCTTATT CTTCCATTTG GTGATCAGTT CTGGTTTAGG 
TACCTCTTAG GTTGGTTGAT GCCTCCAAAG GTCTCTCTTC TTAAGGCCAC TCAAGGTGAA 
GCTATCAGGA ACTATTACCA TGATATGCAT GTTATTCAGG ATATGCTTGT TCCTCTTTAC 
AAGGTTGGCG ATGCACTCGA ATGGGTCCAC CGCGAAATGG AGGTGTATCC AATTTGGCTT 
TGCCCACACA AACTCTTCAA GCAGCCAATC AAAGGCCAAA TCTACCCAGA GCCAGGCTTC 
GAGTACGAAA ACAGACAAGG AGACACAGAA GATGCACAGA TGTACACTGA TGTTGGAGTC 
TACTACGCAC CTGGCTGTGT CCTAAGAGGT GAAGAGTTTG ATGGATCAGA AGCAGTGCGT 
AGGATGGAGA AATGGCTGAT AGAGAACCAT GGATTCCAGC CTCAGTACGC GGTGTCTGAG 
CTCGACGAGA AGAGCTTCTG GAGAATGTTT AATGGTGAAT TGTATGAGGA GTGCCGCAAG 
AAGTATAGAG CTATTGGAAC GTTCATGAGT GTTTACTACA AGTCCAAGAA AGGAAGGAAG 
ACTGAGAAAG AAGTTAGAGA AGCCGAACAA GCTCATCTCG AAACTGCTTA TGCCGAGGCA 
GATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DWF1 ( NM_112872.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_188616.1 Arabidopsis thaliana cell elongation protein / DWARF1 / DIMINUTO (DIM) (DWF1), mRNA.

Price & Availability↑

CloneID	OAb11868
Clone ID Related Accession (Same CDS sequence)	NM_180285.4 , NM_001338420.1 , NM_112872.3
Accession Version	NM_112872.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	cell elongation protein / DWARF1 / DIMINUTO (DIM)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_112872.2.

ATGTCGGATC TTCAGACACC GCTTGTGAGG CCCAAGAGGA AGAAGACTTG GGTTGATTAC 
TTTGTCAAGT TCAGATGGAT CATTGTCATC TTCATCGTCC TTCCATTCTC AGCCACATTC 
TACTTCCTCA TCTACCTCGG GGACATGTGG TCAGAGTCCA AGTCCTTTGA GAAACGTCAG 
AAGGAACACG ACGAGAATGT CAAGAAAGTC ATCAAAAGGC TTAAGGGTAG GGATGCTTCC 
AAGGACGGGC TTGTCTGCAC TGCTCGTAAG CCCTGGATCG CTGTTGGAAT GAGGAACGTT 
GACTACAAGA GAGCCCGGCA TTTCGAGGTT GACTTGGGGG AGTTCCGTAA CATCCTTGAG 
ATCAACAAGG AGAAGATGAC TGCTAGAGTG GAGCCTCTTG TTAACATGGG ACAGATTTCC 
CGTGCTACCG TCCCAATGAA CCTGTCTCTC GCTGTTGTTG CTGAGCTTGA TGACCTTACC 
GTTGGTGGAC TTATCAATGG ATATGGTATT GAAGGAAGCT CTCACATCTA CGGTTTGTTT 
GCTGATACCG TTGAGGCTTA CGAGATTGTT CTTGCGGGTG GAGAGCTTGT CCGCGCCACA 
AGGGATAATG AGTATTCTGA TCTTTACTAC GCAATCCCGT GGTCGCAAGG AACTCTTGGA 
CTCCTTGTAG CTGCTGAGAT CAGGCTTATT AAAGTCAAGG AGTACATGAG ACTCACTTAC 
ATACCAGTCA AGGGTGATCT TCAAGCCTTA GCTCAAGGTT ACATTGATTC TTTTGCTCCC 
AAAGACGGTG ACAAGTCGAA AATCCCGGAT TTCGTCGAAG GCATGGTTTA CAATCCAACG 
GAAGGAGTGA TGATGGTTGG AACATATGCA TCTAAAGAAG AGGCAAAGAA GAAAGGGAAC 
AAAATCAACA ATGTGGGATG GTGGTTCAAG CCGTGGTTCT ACCAGCACGC GCAGACCGCC 
CTGAAAAAGG GACAGTTTGT TGAGTACATC CCAACTCGTG AATACTACCA CAGGCACACA 
AGGTGCTTGT ACTGGGAAGG GAAGCTTATT CTTCCATTTG GTGATCAGTT CTGGTTTAGG 
TACCTCTTAG GTTGGTTGAT GCCTCCAAAG GTCTCTCTTC TTAAGGCCAC TCAAGGTGAA 
GCTATCAGGA ACTATTACCA TGATATGCAT GTTATTCAGG ATATGCTTGT TCCTCTTTAC 
AAGGTTGGCG ATGCACTCGA ATGGGTCCAC CGCGAAATGG AGGTGTATCC AATTTGGCTT 
TGCCCACACA AACTCTTCAA GCAGCCAATC AAAGGCCAAA TCTACCCAGA GCCAGGCTTC 
GAGTACGAAA ACAGACAAGG AGACACAGAA GATGCACAGA TGTACACTGA TGTTGGAGTC 
TACTACGCAC CTGGCTGTGT CCTAAGAGGT GAAGAGTTTG ATGGATCAGA AGCAGTGCGT 
AGGATGGAGA AATGGCTGAT AGAGAACCAT GGATTCCAGC CTCAGTACGC GGTGTCTGAG 
CTCGACGAGA AGAGCTTCTG GAGAATGTTT AATGGTGAAT TGTATGAGGA GTGCCGCAAG 
AAGTATAGAG CTATTGGAAC GTTCATGAGT GTTTACTACA AGTCCAAGAA AGGAAGGAAG 
ACTGAGAAAG AAGTTAGAGA AGCCGAACAA GCTCATCTCG AAACTGCTTA TGCCGAGGCA 
GATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_188616.1
CDS	620..2305
Translation	MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQKEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILEINKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTYIPVKGDLQALAQGYIDSFAPKDGDKSKIPDFVEGMVYNPTEGVMMVGTYASKEEAKKKGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQFWFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYPIWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSEAVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKKGRKTEKEVREAEQAHLETAYAEAD

Target ORF information:

RefSeq Version	NM_112872.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana cell elongation protein / DWARF1 / DIMINUTO (DIM) (DWF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_112872.3

ORF Insert Sequence:

ATGTCGGATC TTCAGACACC GCTTGTGAGG CCCAAGAGGA AGAAGACTTG GGTTGATTAC 
TTTGTCAAGT TCAGATGGAT CATTGTCATC TTCATCGTCC TTCCATTCTC AGCCACATTC 
TACTTCCTCA TCTACCTCGG GGACATGTGG TCAGAGTCCA AGTCCTTTGA GAAACGTCAG 
AAGGAACACG ACGAGAATGT CAAGAAAGTC ATCAAAAGGC TTAAGGGTAG GGATGCTTCC 
AAGGACGGGC TTGTCTGCAC TGCTCGTAAG CCCTGGATCG CTGTTGGAAT GAGGAACGTT 
GACTACAAGA GAGCCCGGCA TTTCGAGGTT GACTTGGGGG AGTTCCGTAA CATCCTTGAG 
ATCAACAAGG AGAAGATGAC TGCTAGAGTG GAGCCTCTTG TTAACATGGG ACAGATTTCC 
CGTGCTACCG TCCCAATGAA CCTGTCTCTC GCTGTTGTTG CTGAGCTTGA TGACCTTACC 
GTTGGTGGAC TTATCAATGG ATATGGTATT GAAGGAAGCT CTCACATCTA CGGTTTGTTT 
GCTGATACCG TTGAGGCTTA CGAGATTGTT CTTGCGGGTG GAGAGCTTGT CCGCGCCACA 
AGGGATAATG AGTATTCTGA TCTTTACTAC GCAATCCCGT GGTCGCAAGG AACTCTTGGA 
CTCCTTGTAG CTGCTGAGAT CAGGCTTATT AAAGTCAAGG AGTACATGAG ACTCACTTAC 
ATACCAGTCA AGGGTGATCT TCAAGCCTTA GCTCAAGGTT ACATTGATTC TTTTGCTCCC 
AAAGACGGTG ACAAGTCGAA AATCCCGGAT TTCGTCGAAG GCATGGTTTA CAATCCAACG 
GAAGGAGTGA TGATGGTTGG AACATATGCA TCTAAAGAAG AGGCAAAGAA GAAAGGGAAC 
AAAATCAACA ATGTGGGATG GTGGTTCAAG CCGTGGTTCT ACCAGCACGC GCAGACCGCC 
CTGAAAAAGG GACAGTTTGT TGAGTACATC CCAACTCGTG AATACTACCA CAGGCACACA 
AGGTGCTTGT ACTGGGAAGG GAAGCTTATT CTTCCATTTG GTGATCAGTT CTGGTTTAGG 
TACCTCTTAG GTTGGTTGAT GCCTCCAAAG GTCTCTCTTC TTAAGGCCAC TCAAGGTGAA 
GCTATCAGGA ACTATTACCA TGATATGCAT GTTATTCAGG ATATGCTTGT TCCTCTTTAC 
AAGGTTGGCG ATGCACTCGA ATGGGTCCAC CGCGAAATGG AGGTGTATCC AATTTGGCTT 
TGCCCACACA AACTCTTCAA GCAGCCAATC AAAGGCCAAA TCTACCCAGA GCCAGGCTTC 
GAGTACGAAA ACAGACAAGG AGACACAGAA GATGCACAGA TGTACACTGA TGTTGGAGTC 
TACTACGCAC CTGGCTGTGT CCTAAGAGGT GAAGAGTTTG ATGGATCAGA AGCAGTGCGT 
AGGATGGAGA AATGGCTGAT AGAGAACCAT GGATTCCAGC CTCAGTACGC GGTGTCTGAG 
CTCGACGAGA AGAGCTTCTG GAGAATGTTT AATGGTGAAT TGTATGAGGA GTGCCGCAAG 
AAGTATAGAG CTATTGGAAC GTTCATGAGT GTTTACTACA AGTCCAAGAA AGGAAGGAAG 
ACTGAGAAAG AAGTTAGAGA AGCCGAACAA GCTCATCTCG AAACTGCTTA TGCCGAGGCA 
GATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Nature408(6814)820-2(2000 Dec)
Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,

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DWF1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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