PREP1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	PREP1
Entrez Gene ID	821451
Full Name	presequence protease 1
Synonyms	ATPREP1,ATZNMP,presequence protease 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_112804.5	NP_188548.2	presequence protease 1
NM_001203003.1	NP_001189932.1	presequence protease 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PITRM1	XP_001140483.1
Schizosaccharomyces pombe (fission yeast)	SPBC119.17	NP_595299.2
Magnaporthe oryzae (rice blast fungus)	MGG_02440	XP_003709229.1
Neurospora crassa	NCU01272	XP_960750.1
Arabidopsis thaliana (thale cress)	PREP1	NP_188548.2
Bos taurus (cattle)	PITRM1	XP_002692237.2
Gallus gallus (chicken)	PITRM1	XP_418564.2
Arabidopsis thaliana (thale cress)	PREP2	NP_850962.1
Homo sapiens (human)	PITRM1	NP_055704.2
Canis lupus familiaris (dog)	PITRM1	XP_535200.2
Rattus norvegicus (Norway rat)	Pitrm1	NP_001100833.1
	PITRM1	XP_001102165.1
Drosophila melanogaster (fruit fly)	CG3107	NP_001036470.1
Mus musculus (house mouse)	Pitrm1	NP_660113.1
Danio rerio (zebrafish)	pitrm1	NP_998652.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006616	XP_316646.4
Eremothecium gossypii	AGOS_ADR184W	NP_984280.2
Xenopus tropicalis (tropical clawed frog)	pitrm1	NP_001039262.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that the AtPreP presequence has the capacity to target GFP into the mitochondrial matrix of all three species.
Title: Targeting capacity and conservation of PreP homologues localization in mitochondria of different species.

The AtPreP peptidasome is required for efficient plant growth and organelle function particularly during early development.
Title: Deletion of an organellar peptidasome PreP affects early development in Arabidopsis thaliana.

AtPreP functions as a peptidasome that degrades targeting peptides and other small unstructured peptides. In addition to Zn located in the catalytic site, AtPreP also contains two Mg-binding sites. [PreP1]
Title: Binding of divalent cations is essential for the activity of the organellar peptidasome in Arabidopsis thaliana, AtPreP.

AtPreP1 and AtPreP2 genes are differentially expressed in Arabidopsis
Title: Two novel mitochondrial and chloroplastic targeting-peptide-degrading peptidasomes in A. thaliana, AtPreP1 and AtPreP2.

AtPrePI and AtPrePII are metalloproteases that degrade targeting peptides in mitochondria and chloroplasts. AtPrePI showed a preference for the N-terminal amphiphilic α-helix and positively charged amino acid residues.
Title: Catalysis, subcellular localization, expression and evolution of the targeting peptides degrading protease, AtPreP2.

The following PREP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PREP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb11816	NM_112804.5 Latest version!	Arabidopsis thaliana presequence protease 1 (PREP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OAb30125	NM_001203003.1 Latest version!	Arabidopsis thaliana presequence protease 1 (PREP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PREP1 ( NM_112804.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_188548.2 Arabidopsis thaliana presequence protease 1 (PREP1), mRNA.

Price & Availability↑

CloneID	OAb11816
Clone ID Related Accession (Same CDS sequence)	NM_112804.5
Accession Version	NM_112804.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	presequence protease 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_112804.4.

ATGCTCCGAA CCGTCTCTTG TTTAGCTTCG CGCAGCTCGT CCTCTCTATT CTTTCGCTTC 
TTTCGTCAGT TCCCTCGCTC TTACATGTCT CTCACATCGT CGACGGCAGC TCTCCGTGTC 
CCCAGTCGCA ATCTTCGCCG AATTTCCTCT CCCTCAGTCG CCGGTAGACG TCTTTTGCTC 
CGACGAGGTT TGAGGATTCC CTCCGCCGCA GTTCGCAGTG TCAATGGGCA GTTCTCTCGC 
CTCTCTGTTC GCGCCGTCGC CACACAACCC GCTCCACTTT ATCCTGATGT AGGTCAAGAT 
GAGGCTGAGA AGCTTGGATT TGAAAAAGTG TCGGAAGAAT TCATCTCAGA GTGTAAATCA 
AAGGCGATAC TCTTCAAACA CAAGAAAACT GGTTGCGAGG TGATGTCTGT GTCAAATGAG 
GATGAGAACA AGGTCTTCGG TGTTGTTTTC AGAACTCCTC CGAAGGATTC CACTGGCATT 
CCGCACATAC TGGAACATAG CGTTCTATGT GGGTCAAGAA AGTACCCGGT AAAAGAGCCA 
TTTGTTGAAC TACTCAAGGG AAGTTTGCAT ACTTTTCTAA ATGCATTCAC ATATCCTGAT 
AGGACCTGCT ACCCAGTTGC TTCCACCAAT ACAAAGGATT TTTACAATCT TGTCGATGTA 
TATTTGGATG CTGTCTTCTT CCCCAAGTGT GTGGATGACG CTCACACCTT TCAGCAAGAG 
GGCTGGCACT ATGAACTTAA TGATCCCTCG GAAGACATAT CTTACAAAGG TGTTGTTTTT 
AATGAGATGA AAGGGGTCTA TTCACAGCCT GATAATATTT TAGGGCGAAT TGCTCAACAG 
GCCTTATCCC CAGAAAATAC ATATGGTGTT GACAGTGGAG GTGATCCAAA AGATATTCCT 
AATCTGACAT TTGAGGAATT CAAGGAGTTC CACCGTCAAT ATTATCACCC AAGCAACGCC 
AGGATCTGGT TCTATGGAGA TGATGATCCC GTTCACCGCC TCCGTGTCTT GAGTGAATAC 
TTGGATATGT TTGAAGCAAG TCCATCTCCC AATAGTTCAA AGATTAAATT TCAAAAGCTA 
TTCTCTGAGC CTGTCAGACT GGTTGAGAAA TATCCTGCTG GTCGAGATGG TGACCTTAAA 
AAGAAGCATA TGTTGTGCGT TAACTGGCTA TTGTCTGAGA AGCCCTTGGA CTTGCAAACT 
CAACTTGCAC TTGGCTTCTT GGATCATCTT ATGCTGGGGA CTCCTGCTTC ACCACTAAGA 
AAGATCTTAT TGGAAAGCGG TTTAGGAGAA GCTCTTGTCA GTAGTGGGTT GTCAGACGAA 
CTCTTGCAGC CCCAGTTCGG TATTGGTTTG AAAGGTGTGT CCGAAGAGAA TGTTCAAAAG 
GTTGAAGAGT TGATCATGGA TACTCTGAAA AAGTTGGCAG AAGAAGGGTT TGACAATGAT 
GCTGTGGAGG CATCCATGAA TACAATTGAG TTTTCTTTGA GGGAAAACAA CACAGGATCT 
TTCCCTCGTG GTTTATCACT TATGCTCCAA TCTATTTCAA AATGGATATA CGATATGGAT 
CCTTTTGAGC CATTAAAGTA TACGGAACCA TTGAAGGCCC TGAAAACCAG AATAGCTGAG 
GAGGGATCCA AGGCTGTCTT TTCCCCACTG ATAGAGAAGC TTATCTTGAA CAACTCGCAT 
CGTGTTACCA TAGAGATGCA GCCTGATCCT GAAAAAGCTA CTCAAGAGGA AGTGGAAGAG 
AAAAATATCC TGGAAAAAGT TAAAGCAGCT ATGACAGAAG AAGATCTTGC AGAGCTAGCA 
CGTGCTACAG AGGAGCTGAA ATTGAAACAA GAAACTCCTG ACCCTCCTGA AGCATTGAGA 
TGTGTCCCAA GTTTGAACTT GGGTGACATT CCAAAAGAAC CTACTTATGT TCCCACCGAG 
GTTGGAGATA TTAATGGTGT GAAGGTTTTG CGGCATGACC TTTTCACCAA TGATATTATC 
TATACTGAAG TAGTCTTTGA TATTGGTTCG CTGAAACATG AGCTTCTTCC ACTAGTACCA 
CTTTTCTGTC AATCATTACT GGAGATGGGC ACAAAAGATT TGACTTTTGT TCAACTTAAT 
CAGTTGATTG GAAGGAAAAC GGGAGGGATA TCAGTTTATC CCCTTACATC ATCAGTGAGG 
GGTAAGGATG AACCATGCAG CAAAATTATC GTACGCGGAA AATCCATGGC TGGGCGTGCT 
GACGACCTTT TTAACCTGAT GAACTGCTTG TTGCAAGAGG TTCAATTTAC AGATCAGCAG 
AGGTTTAAAC AGTTTGTCTC TCAAAGCAGA GCACGGATGG AGAACCGATT GAGGGGAAGT 
GGTCATGGAA TTGCTGCGGC GAGGATGGAT GCAATGTTGA ACATTGCTGG ATGGATGTCT 
GAACAGATGG GTGGTCTCAG CTATCTTGAA TTTTTACATA CTCTTGAAAA GAAAGTGGAT 
GAAGACTGGG AAGGAATTTC ATCCTCGCTT GAGGAGATCA GAAGATCTCT CCTTGCTAGA 
AACGGTTGCA TAGTTAATAT GACTGCTGAT GGGAAGTCTC TCACAAATGT CGAAAAATCT 
GTTGCAAAAT TTCTAGATTT ACTCCCTGAG AACCCGTCTG GTGGGCTTGT CACTTGGGAT 
GGTCGACTTC CTCTGAGAAA TGAAGCCATT GTAATACCAA CCCAGGTAAA CTATGTTGGA 
AAAGCGGGTA ACATATACAG CACCGGGTAT GAGCTTGATG GCAGTGCGTA TGTTATATCA 
AAGCATATTA GCAATACATG GTTATGGGAT CGTGTTCGTG TAAGTGGTGG TGCATATGGA 
GGTTTCTGTG ATTTCGATTC GCATTCAGGA GTATTTTCTT ACTTATCGTA CCGGGATCCA 
AACTTGTTGA AGACACTTGA TATATATGAT GGAACTGGAG ATTTCTTACG TGGCCTTGAT 
GTGGATCAAG AAACACTCAC TAAAGCTATT ATTGGCACCA TCGGTGATGT TGATTCATAC 
CAATTACCTG ATGCCAAAGG TTATAGCAGT TTGTTGAGGC ATTTACTCGG AGTAACTGAC 
GAAGAACGAC AAAGAAAGCG TGAAGAGATA CTAACAACAA GTTTGAAGGA CTTCAAGGAT 
TTCGCACAAG CAATAGATGT GGTTAGAGAT AAAGGTGTTG CGGTGGCTGT GGCATCCGCG 
GAAGACATTG ATGCAGCGAA CAATGAACGC TCCAACTTTT TCGAGGTGAA GAAAGCTCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_188548.2
CDS	291..3533
Translation	MLRTVSCLASRSSSSLFFRFFRQFPRSYMSLTSSTAALRVPSRNLRRISSPSVAGRRLLLRRGLRIPSAAVRSVNGQFSRLSVRAVATQPAPLYPDVGQDEAEKLGFEKVSEEFISECKSKAILFKHKKTGCEVMSVSNEDENKVFGVVFRTPPKDSTGIPHILEHSVLCGSRKYPVKEPFVELLKGSLHTFLNAFTYPDRTCYPVASTNTKDFYNLVDVYLDAVFFPKCVDDAHTFQQEGWHYELNDPSEDISYKGVVFNEMKGVYSQPDNILGRIAQQALSPENTYGVDSGGDPKDIPNLTFEEFKEFHRQYYHPSNARIWFYGDDDPVHRLRVLSEYLDMFEASPSPNSSKIKFQKLFSEPVRLVEKYPAGRDGDLKKKHMLCVNWLLSEKPLDLQTQLALGFLDHLMLGTPASPLRKILLESGLGEALVSSGLSDELLQPQFGIGLKGVSEENVQKVEELIMDTLKKLAEEGFDNDAVEASMNTIEFSLRENNTGSFPRGLSLMLQSISKWIYDMDPFEPLKYTEPLKALKTRIAEEGSKAVFSPLIEKLILNNSHRVTIEMQPDPEKATQEEVEEKNILEKVKAAMTEEDLAELARATEELKLKQETPDPPEALRCVPSLNLGDIPKEPTYVPTEVGDINGVKVLRHDLFTNDIIYTEVVFDIGSLKHELLPLVPLFCQSLLEMGTKDLTFVQLNQLIGRKTGGISVYPLTSSVRGKDEPCSKIIVRGKSMAGRADDLFNLMNCLLQEVQFTDQQRFKQFVSQSRARMENRLRGSGHGIAAARMDAMLNIAGWMSEQMGGLSYLEFLHTLEKKVDEDWEGISSSLEEIRRSLLARNGCIVNMTADGKSLTNVEKSVAKFLDLLPENPSGGLVTWDGRLPLRNEAIVIPTQVNYVGKAGNIYSTGYELDGSAYVISKHISNTWLWDRVRVSGGAYGGFCDFDSHSGVFSYLSYRDPNLLKTLDIYDGTGDFLRGLDVDQETLTKAIIGTIGDVDSYQLPDAKGYSSLLRHLLGVTDEERQRKREEILTTSLKDFKDFAQAIDVVRDKGVAVAVASAEDIDAANNERSNFFEVKKAL

Target ORF information:

RefSeq Version	NM_112804.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana presequence protease 1 (PREP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_112804.5

ORF Insert Sequence:

ATGCTCCGAA CCGTCTCTTG TTTAGCTTCG CGCAGCTCGT CCTCTCTATT CTTTCGCTTC 
TTTCGTCAGT TCCCTCGCTC TTACATGTCT CTCACATCGT CGACGGCAGC TCTCCGTGTC 
CCCAGTCGCA ATCTTCGCCG AATTTCCTCT CCCTCAGTCG CCGGTAGACG TCTTTTGCTC 
CGACGAGGTT TGAGGATTCC CTCCGCCGCA GTTCGCAGTG TCAATGGGCA GTTCTCTCGC 
CTCTCTGTTC GCGCCGTCGC CACACAACCC GCTCCACTTT ATCCTGATGT AGGTCAAGAT 
GAGGCTGAGA AGCTTGGATT TGAAAAAGTG TCGGAAGAAT TCATCTCAGA GTGTAAATCA 
AAGGCGATAC TCTTCAAACA CAAGAAAACT GGTTGCGAGG TGATGTCTGT GTCAAATGAG 
GATGAGAACA AGGTCTTCGG TGTTGTTTTC AGAACTCCTC CGAAGGATTC CACTGGCATT 
CCGCACATAC TGGAACATAG CGTTCTATGT GGGTCAAGAA AGTACCCGGT AAAAGAGCCA 
TTTGTTGAAC TACTCAAGGG AAGTTTGCAT ACTTTTCTAA ATGCATTCAC ATATCCTGAT 
AGGACCTGCT ACCCAGTTGC TTCCACCAAT ACAAAGGATT TTTACAATCT TGTCGATGTA 
TATTTGGATG CTGTCTTCTT CCCCAAGTGT GTGGATGACG CTCACACCTT TCAGCAAGAG 
GGCTGGCACT ATGAACTTAA TGATCCCTCG GAAGACATAT CTTACAAAGG TGTTGTTTTT 
AATGAGATGA AAGGGGTCTA TTCACAGCCT GATAATATTT TAGGGCGAAT TGCTCAACAG 
GCCTTATCCC CAGAAAATAC ATATGGTGTT GACAGTGGAG GTGATCCAAA AGATATTCCT 
AATCTGACAT TTGAGGAATT CAAGGAGTTC CACCGTCAAT ATTATCACCC AAGCAACGCC 
AGGATCTGGT TCTATGGAGA TGATGATCCC GTTCACCGCC TCCGTGTCTT GAGTGAATAC 
TTGGATATGT TTGAAGCAAG TCCATCTCCC AATAGTTCAA AGATTAAATT TCAAAAGCTA 
TTCTCTGAGC CTGTCAGACT GGTTGAGAAA TATCCTGCTG GTCGAGATGG TGACCTTAAA 
AAGAAGCATA TGTTGTGCGT TAACTGGCTA TTGTCTGAGA AGCCCTTGGA CTTGCAAACT 
CAACTTGCAC TTGGCTTCTT GGATCATCTT ATGCTGGGGA CTCCTGCTTC ACCACTAAGA 
AAGATCTTAT TGGAAAGCGG TTTAGGAGAA GCTCTTGTCA GTAGTGGGTT GTCAGACGAA 
CTCTTGCAGC CCCAGTTCGG TATTGGTTTG AAAGGTGTGT CCGAAGAGAA TGTTCAAAAG 
GTTGAAGAGT TGATCATGGA TACTCTGAAA AAGTTGGCAG AAGAAGGGTT TGACAATGAT 
GCTGTGGAGG CATCCATGAA TACAATTGAG TTTTCTTTGA GGGAAAACAA CACAGGATCT 
TTCCCTCGTG GTTTATCACT TATGCTCCAA TCTATTTCAA AATGGATATA CGATATGGAT 
CCTTTTGAGC CATTAAAGTA TACGGAACCA TTGAAGGCCC TGAAAACCAG AATAGCTGAG 
GAGGGATCCA AGGCTGTCTT TTCCCCACTG ATAGAGAAGC TTATCTTGAA CAACTCGCAT 
CGTGTTACCA TAGAGATGCA GCCTGATCCT GAAAAAGCTA CTCAAGAGGA AGTGGAAGAG 
AAAAATATCC TGGAAAAAGT TAAAGCAGCT ATGACAGAAG AAGATCTTGC AGAGCTAGCA 
CGTGCTACAG AGGAGCTGAA ATTGAAACAA GAAACTCCTG ACCCTCCTGA AGCATTGAGA 
TGTGTCCCAA GTTTGAACTT GGGTGACATT CCAAAAGAAC CTACTTATGT TCCCACCGAG 
GTTGGAGATA TTAATGGTGT GAAGGTTTTG CGGCATGACC TTTTCACCAA TGATATTATC 
TATACTGAAG TAGTCTTTGA TATTGGTTCG CTGAAACATG AGCTTCTTCC ACTAGTACCA 
CTTTTCTGTC AATCATTACT GGAGATGGGC ACAAAAGATT TGACTTTTGT TCAACTTAAT 
CAGTTGATTG GAAGGAAAAC GGGAGGGATA TCAGTTTATC CCCTTACATC ATCAGTGAGG 
GGTAAGGATG AACCATGCAG CAAAATTATC GTACGCGGAA AATCCATGGC TGGGCGTGCT 
GACGACCTTT TTAACCTGAT GAACTGCTTG TTGCAAGAGG TTCAATTTAC AGATCAGCAG 
AGGTTTAAAC AGTTTGTCTC TCAAAGCAGA GCACGGATGG AGAACCGATT GAGGGGAAGT 
GGTCATGGAA TTGCTGCGGC GAGGATGGAT GCAATGTTGA ACATTGCTGG ATGGATGTCT 
GAACAGATGG GTGGTCTCAG CTATCTTGAA TTTTTACATA CTCTTGAAAA GAAAGTGGAT 
GAAGACTGGG AAGGAATTTC ATCCTCGCTT GAGGAGATCA GAAGATCTCT CCTTGCTAGA 
AACGGTTGCA TAGTTAATAT GACTGCTGAT GGGAAGTCTC TCACAAATGT CGAAAAATCT 
GTTGCAAAAT TTCTAGATTT ACTCCCTGAG AACCCGTCTG GTGGGCTTGT CACTTGGGAT 
GGTCGACTTC CTCTGAGAAA TGAAGCCATT GTAATACCAA CCCAGGTAAA CTATGTTGGA 
AAAGCGGGTA ACATATACAG CACCGGGTAT GAGCTTGATG GCAGTGCGTA TGTTATATCA 
AAGCATATTA GCAATACATG GTTATGGGAT CGTGTTCGTG TAAGTGGTGG TGCATATGGA 
GGTTTCTGTG ATTTCGATTC GCATTCAGGA GTATTTTCTT ACTTATCGTA CCGGGATCCA 
AACTTGTTGA AGACACTTGA TATATATGAT GGAACTGGAG ATTTCTTACG TGGCCTTGAT 
GTGGATCAAG AAACACTCAC TAAAGCTATT ATTGGCACCA TCGGTGATGT TGATTCATAC 
CAATTACCTG ATGCCAAAGG TTATAGCAGT TTGTTGAGGC ATTTACTCGG AGTAACTGAC 
GAAGAACGAC AAAGAAAGCG TGAAGAGATA CTAACAACAA GTTTGAAGGA CTTCAAGGAT 
TTCGCACAAG CAATAGATGT GGTTAGAGAT AAAGGTGTTG CGGTGGCTGT GGCATCCGCG 
GAAGACATTG ATGCAGCGAA CAATGAACGC TCCAACTTTT TCGAGGTGAA GAAAGCTCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PREP1 ( NM_001203003.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001189932.1 Arabidopsis thaliana presequence protease 1 (PREP1), mRNA.

Price & Availability↑

CloneID	OAb30125
Clone ID Related Accession (Same CDS sequence)	NM_001203003.1
Accession Version	NM_001203003.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	presequence protease 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074).

ATGCTCCGAA CCGTCTCTTG TTTAGCTTCG CGCAGCTCGT CCTCTCTATT CTTTCGCTTC 
TTTCGTCAGT TCCCTCGCTC TTACATGTCT CTCACATCGT CGACGGCAGC TCTCCGTGTC 
CCCAGTCGCA ATCTTCGCCG AATTTCCTCT CCCTCAGTCG CCGGTAGACG TCTTTTGCTC 
CGACGAGGTT TGAGGATTCC CTCCGCCGCA GTTCGCAGTG TCAATGGGCA GTTCTCTCGC 
CTCTCTGTTC GCGCCGTCGC CACACAACCC GCTCCACTTT ATCCTGATGT AGGTCAAGAT 
GAGGCTGAGA AGCTTGGATT TGAAAAAGTG TCGGAAGAAT TCATCTCAGA GTGTAAATCA 
AAGGCGATAC TCTTCAAACA CAAGAAAACT GGTTGCGAGG TGATGTCTGT GTCAAATGAG 
GATGAGAACA AGGTCTTCGG TGTTGTTTTC AGAACTCCTC CGAAGGATTC CACTGGCATT 
CCGCACATAC TGGAACATAG CGTTCTATGT GGGTCAAGAA AGTACCCGGT AAAAGAGCCA 
TTTGTTGAAC TACTCAAGGG AAGTTTGCAT ACTTTTCTAA ATGCATTCAC ATATCCTGAT 
AGGACCTGCT ACCCAGTTGC TTCCACCAAT ACAAAGGATT TTTACAATCT TGTCGATGTA 
TATTTGGATG CTGTCTTCTT CCCCAAGTGT GTGGATGACG CTCACACCTT TCAGCAAGAG 
GGCTGGCACT ATGAACTTAA TGATCCCTCG GAAGACATAT CTTACAAAGC TACTTCAGAT 
ACCCACTCAT CCTATCTCAT TGTTGATGCC TTATCCCCAG AAAATACATA TGGTGTTGAC 
AGTGGAGGTG ATCCAAAAGA TATTCCTAAT CTGACATTTG AGGAATTCAA GGAGTTCCAC 
CGTCAATATT ATCACCCAAG CAACGCCAGG ATCTGGTTCT ATGGAGATGA TGATCCCGTT 
CACCGCCTCC GTGTCTTGAG TGAATACTTG GATATGTTTG AAGCAAGTCC ATCTCCCAAT 
AGTTCAAAGA TTAAATTTCA AAAGCTATTC TCTGAGCCTG TCAGACTGGT TGAGAAATAT 
CCTGCTGGTC GAGATGGTGA CCTTAAAAAG AAGCATATGT TGTGCGTTAA CTGGCTATTG 
TCTGAGAAGC CCTTGGACTT GCAAACTCAA CTTGCACTTG GCTTCTTGGA TCATCTTATG 
CTGGGGACTC CTGCTTCACC ACTAAGAAAG ATCTTATTGG AAAGCGGTTT AGGAGAAGCT 
CTTGTCAGTA GTGGGTTGTC AGACGAACTC TTGCAGCCCC AGTTCGGTAT TGGTTTGAAA 
GGTGTGTCCG AAGAGAATGT TCAAAAGGTT GAAGAGTTGA TCATGGATAC TCTGAAAAAG 
TTGGCAGAAG AAGGGTTTGA CAATGATGCT GTGGAGGCAT CCATGAATAC AATTGAGTTT 
TCTTTGAGGG AAAACAACAC AGGATCTTTC CCTCGTGGTT TATCACTTAT GCTCCAATCT 
ATTTCAAAAT GGATATACGA TATGGATCCT TTTGAGCCAT TAAAGTATAC GGAACCATTG 
AAGGCCCTGA AAACCAGAAT AGCTGAGGAG GGATCCAAGG CTGTCTTTTC CCCACTGATA 
GAGAAGCTTA TCTTGAACAA CTCGCATCGT GTTACCATAG AGATGCAGCC TGATCCTGAA 
AAAGCTACTC AAGAGGAAGT GGAAGAGAAA AATATCCTGG AAAAAGTTAA AGCAGCTATG 
ACAGAAGAAG ATCTTGCAGA GCTAGCACGT GCTACAGAGG AGCTGAAATT GAAACAAGAA 
ACTCCTGACC CTCCTGAAGC ATTGAGATGT GTCCCAAGTT TGAACTTGGG TGACATTCCA 
AAAGAACCTA CTTATGTTCC CACCGAGGTT GGAGATATTA ATGGTGTGAA GGTTTTGCGG 
CATGACCTTT TCACCAATGA TATTATCTAT ACTGAAGTAG TCTTTGATAT TGGTTCGCTG 
AAACATGAGC TTCTTCCACT AGTACCACTT TTCTGTCAAT CATTACTGGA GATGGGCACA 
AAAGATTTGA CTTTTGTTCA ACTTAATCAG TTGATTGGAA GGAAAACGGG AGGGATATCA 
GTTTATCCCC TTACATCATC AGTGAGGGGT AAGGATGAAC CATGCAGCAA AATTATCGTA 
CGCGGAAAAT CCATGGCTGG GCGTGCTGAC GACCTTTTTA ACCTGATGAA CTGCTTGTTG 
CAAGAGGTTC AATTTACAGA TCAGCAGAGG TTTAAACAGT TTGTCTCTCA AAGCAGAGCA 
CGGATGGAGA ACCGATTGAG GGGAAGTGGT CATGGAATTG CTGCGGCGAG GATGGATGCA 
ATGTTGAACA TTGCTGGATG GATGTCTGAA CAGATGGGTG GTCTCAGCTA TCTTGAATTT 
TTACATACTC TTGAAAAGAA AGTGGATGAA GACTGGGAAG GAATTTCATC CTCGCTTGAG 
GAGATCAGAA GATCTCTCCT TGCTAGAAAC GGTTGCATAG TTAATATGAC TGCTGATGGG 
AAGTCTCTCA CAAATGTCGA AAAATCTGTT GCAAAATTTC TAGATTTACT CCCTGAGAAC 
CCGTCTGGTG GGCTTGTCAC TTGGGATGGT CGACTTCCTC TGAGAAATGA AGCCATTGTA 
ATACCAACCC AGGTAAACTA TGTTGGAAAA GCGGGTAACA TATACAGCAC CGGGTATGAG 
CTTGATGGCA GTGCGTATGT TATATCAAAG CATATTAGCA ATACATGGTT ATGGGATCGT 
GTTCGTGTAA GTGGTGGTGC ATATGGAGGT TTCTGTGATT TCGATTCGCA TTCAGGAGTA 
TTTTCTTACT TATCGTACCG GGATCCAAAC TTGTTGAAGA CACTTGATAT ATATGATGGA 
ACTGGAGATT TCTTACGTGG CCTTGATGTG GATCAAGAAA CACTCACTAA AGCTATTATT 
GGCACCATCG GTGATGTTGA TTCATACCAA TTACCTGATG CCAAAGGTTA TAGCAGTTTG 
TTGAGGCATT TACTCGGAGT AACTGACGAA GAACGACAAA GAAAGCGTGA AGAGATACTA 
ACAACAAGTT TGAAGGACTT CAAGGATTTC GCACAAGCAA TAGATGTGGT TAGAGATAAA 
GGTGTTGCGG TGGCTGTGGC ATCCGCGGAA GACATTGATG CAGCGAACAA TGAACGCTCC 
AACTTTTTCG AGGTGAAGAA AGCTCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189932.1
CDS	95..3304
Translation	MLRTVSCLASRSSSSLFFRFFRQFPRSYMSLTSSTAALRVPSRNLRRISSPSVAGRRLLLRRGLRIPSAAVRSVNGQFSRLSVRAVATQPAPLYPDVGQDEAEKLGFEKVSEEFISECKSKAILFKHKKTGCEVMSVSNEDENKVFGVVFRTPPKDSTGIPHILEHSVLCGSRKYPVKEPFVELLKGSLHTFLNAFTYPDRTCYPVASTNTKDFYNLVDVYLDAVFFPKCVDDAHTFQQEGWHYELNDPSEDISYKATSDTHSSYLIVDALSPENTYGVDSGGDPKDIPNLTFEEFKEFHRQYYHPSNARIWFYGDDDPVHRLRVLSEYLDMFEASPSPNSSKIKFQKLFSEPVRLVEKYPAGRDGDLKKKHMLCVNWLLSEKPLDLQTQLALGFLDHLMLGTPASPLRKILLESGLGEALVSSGLSDELLQPQFGIGLKGVSEENVQKVEELIMDTLKKLAEEGFDNDAVEASMNTIEFSLRENNTGSFPRGLSLMLQSISKWIYDMDPFEPLKYTEPLKALKTRIAEEGSKAVFSPLIEKLILNNSHRVTIEMQPDPEKATQEEVEEKNILEKVKAAMTEEDLAELARATEELKLKQETPDPPEALRCVPSLNLGDIPKEPTYVPTEVGDINGVKVLRHDLFTNDIIYTEVVFDIGSLKHELLPLVPLFCQSLLEMGTKDLTFVQLNQLIGRKTGGISVYPLTSSVRGKDEPCSKIIVRGKSMAGRADDLFNLMNCLLQEVQFTDQQRFKQFVSQSRARMENRLRGSGHGIAAARMDAMLNIAGWMSEQMGGLSYLEFLHTLEKKVDEDWEGISSSLEEIRRSLLARNGCIVNMTADGKSLTNVEKSVAKFLDLLPENPSGGLVTWDGRLPLRNEAIVIPTQVNYVGKAGNIYSTGYELDGSAYVISKHISNTWLWDRVRVSGGAYGGFCDFDSHSGVFSYLSYRDPNLLKTLDIYDGTGDFLRGLDVDQETLTKAIIGTIGDVDSYQLPDAKGYSSLLRHLLGVTDEERQRKREEILTTSLKDFKDFAQAIDVVRDKGVAVAVASAEDIDAANNERSNFFEVKKAL

Target ORF information:

RefSeq Version	NM_001203003.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana presequence protease 1 (PREP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001203003.1

ORF Insert Sequence:

ATGCTCCGAA CCGTCTCTTG TTTAGCTTCG CGCAGCTCGT CCTCTCTATT CTTTCGCTTC 
TTTCGTCAGT TCCCTCGCTC TTACATGTCT CTCACATCGT CGACGGCAGC TCTCCGTGTC 
CCCAGTCGCA ATCTTCGCCG AATTTCCTCT CCCTCAGTCG CCGGTAGACG TCTTTTGCTC 
CGACGAGGTT TGAGGATTCC CTCCGCCGCA GTTCGCAGTG TCAATGGGCA GTTCTCTCGC 
CTCTCTGTTC GCGCCGTCGC CACACAACCC GCTCCACTTT ATCCTGATGT AGGTCAAGAT 
GAGGCTGAGA AGCTTGGATT TGAAAAAGTG TCGGAAGAAT TCATCTCAGA GTGTAAATCA 
AAGGCGATAC TCTTCAAACA CAAGAAAACT GGTTGCGAGG TGATGTCTGT GTCAAATGAG 
GATGAGAACA AGGTCTTCGG TGTTGTTTTC AGAACTCCTC CGAAGGATTC CACTGGCATT 
CCGCACATAC TGGAACATAG CGTTCTATGT GGGTCAAGAA AGTACCCGGT AAAAGAGCCA 
TTTGTTGAAC TACTCAAGGG AAGTTTGCAT ACTTTTCTAA ATGCATTCAC ATATCCTGAT 
AGGACCTGCT ACCCAGTTGC TTCCACCAAT ACAAAGGATT TTTACAATCT TGTCGATGTA 
TATTTGGATG CTGTCTTCTT CCCCAAGTGT GTGGATGACG CTCACACCTT TCAGCAAGAG 
GGCTGGCACT ATGAACTTAA TGATCCCTCG GAAGACATAT CTTACAAAGC TACTTCAGAT 
ACCCACTCAT CCTATCTCAT TGTTGATGCC TTATCCCCAG AAAATACATA TGGTGTTGAC 
AGTGGAGGTG ATCCAAAAGA TATTCCTAAT CTGACATTTG AGGAATTCAA GGAGTTCCAC 
CGTCAATATT ATCACCCAAG CAACGCCAGG ATCTGGTTCT ATGGAGATGA TGATCCCGTT 
CACCGCCTCC GTGTCTTGAG TGAATACTTG GATATGTTTG AAGCAAGTCC ATCTCCCAAT 
AGTTCAAAGA TTAAATTTCA AAAGCTATTC TCTGAGCCTG TCAGACTGGT TGAGAAATAT 
CCTGCTGGTC GAGATGGTGA CCTTAAAAAG AAGCATATGT TGTGCGTTAA CTGGCTATTG 
TCTGAGAAGC CCTTGGACTT GCAAACTCAA CTTGCACTTG GCTTCTTGGA TCATCTTATG 
CTGGGGACTC CTGCTTCACC ACTAAGAAAG ATCTTATTGG AAAGCGGTTT AGGAGAAGCT 
CTTGTCAGTA GTGGGTTGTC AGACGAACTC TTGCAGCCCC AGTTCGGTAT TGGTTTGAAA 
GGTGTGTCCG AAGAGAATGT TCAAAAGGTT GAAGAGTTGA TCATGGATAC TCTGAAAAAG 
TTGGCAGAAG AAGGGTTTGA CAATGATGCT GTGGAGGCAT CCATGAATAC AATTGAGTTT 
TCTTTGAGGG AAAACAACAC AGGATCTTTC CCTCGTGGTT TATCACTTAT GCTCCAATCT 
ATTTCAAAAT GGATATACGA TATGGATCCT TTTGAGCCAT TAAAGTATAC GGAACCATTG 
AAGGCCCTGA AAACCAGAAT AGCTGAGGAG GGATCCAAGG CTGTCTTTTC CCCACTGATA 
GAGAAGCTTA TCTTGAACAA CTCGCATCGT GTTACCATAG AGATGCAGCC TGATCCTGAA 
AAAGCTACTC AAGAGGAAGT GGAAGAGAAA AATATCCTGG AAAAAGTTAA AGCAGCTATG 
ACAGAAGAAG ATCTTGCAGA GCTAGCACGT GCTACAGAGG AGCTGAAATT GAAACAAGAA 
ACTCCTGACC CTCCTGAAGC ATTGAGATGT GTCCCAAGTT TGAACTTGGG TGACATTCCA 
AAAGAACCTA CTTATGTTCC CACCGAGGTT GGAGATATTA ATGGTGTGAA GGTTTTGCGG 
CATGACCTTT TCACCAATGA TATTATCTAT ACTGAAGTAG TCTTTGATAT TGGTTCGCTG 
AAACATGAGC TTCTTCCACT AGTACCACTT TTCTGTCAAT CATTACTGGA GATGGGCACA 
AAAGATTTGA CTTTTGTTCA ACTTAATCAG TTGATTGGAA GGAAAACGGG AGGGATATCA 
GTTTATCCCC TTACATCATC AGTGAGGGGT AAGGATGAAC CATGCAGCAA AATTATCGTA 
CGCGGAAAAT CCATGGCTGG GCGTGCTGAC GACCTTTTTA ACCTGATGAA CTGCTTGTTG 
CAAGAGGTTC AATTTACAGA TCAGCAGAGG TTTAAACAGT TTGTCTCTCA AAGCAGAGCA 
CGGATGGAGA ACCGATTGAG GGGAAGTGGT CATGGAATTG CTGCGGCGAG GATGGATGCA 
ATGTTGAACA TTGCTGGATG GATGTCTGAA CAGATGGGTG GTCTCAGCTA TCTTGAATTT 
TTACATACTC TTGAAAAGAA AGTGGATGAA GACTGGGAAG GAATTTCATC CTCGCTTGAG 
GAGATCAGAA GATCTCTCCT TGCTAGAAAC GGTTGCATAG TTAATATGAC TGCTGATGGG 
AAGTCTCTCA CAAATGTCGA AAAATCTGTT GCAAAATTTC TAGATTTACT CCCTGAGAAC 
CCGTCTGGTG GGCTTGTCAC TTGGGATGGT CGACTTCCTC TGAGAAATGA AGCCATTGTA 
ATACCAACCC AGGTAAACTA TGTTGGAAAA GCGGGTAACA TATACAGCAC CGGGTATGAG 
CTTGATGGCA GTGCGTATGT TATATCAAAG CATATTAGCA ATACATGGTT ATGGGATCGT 
GTTCGTGTAA GTGGTGGTGC ATATGGAGGT TTCTGTGATT TCGATTCGCA TTCAGGAGTA 
TTTTCTTACT TATCGTACCG GGATCCAAAC TTGTTGAAGA CACTTGATAT ATATGATGGA 
ACTGGAGATT TCTTACGTGG CCTTGATGTG GATCAAGAAA CACTCACTAA AGCTATTATT 
GGCACCATCG GTGATGTTGA TTCATACCAA TTACCTGATG CCAAAGGTTA TAGCAGTTTG 
TTGAGGCATT TACTCGGAGT AACTGACGAA GAACGACAAA GAAAGCGTGA AGAGATACTA 
ACAACAAGTT TGAAGGACTT CAAGGATTTC GCACAAGCAA TAGATGTGGT TAGAGATAAA 
GGTGTTGCGG TGGCTGTGGC ATCCGCGGAA GACATTGATG CAGCGAACAA TGAACGCTCC 
AACTTTTTCG AGGTGAAGAA AGCTCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Nature408(6814)820-2(2000 Dec)
Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,

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PREP1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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