P5CS1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	P5CS1
Entrez Gene ID	818566
Full Name	delta1-pyrroline-5-carboxylate synthase 1
Synonyms	ATP5CS,T5I7.10,T5I7_10,delta1-pyrroline-5-carboxylate synthase 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_129539.2	NP_181510.1	delta1-pyrroline-5-carboxylate synthase 1
NM_201912.1	NP_973641.1	delta1-pyrroline-5-carboxylate synthase 1
NM_001202786.1	NP_001189715.1	delta1-pyrroline-5-carboxylate synthase 1
NM_001202785.1	NP_001189714.1	delta1-pyrroline-5-carboxylate synthase 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ALDH18A1	XP_005637610.1
Mus musculus (house mouse)	Aldh18a1	NP_062672.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002518	XP_312421.4
Arabidopsis thaliana (thale cress)	P5CS1	NP_181510.1
Homo sapiens (human)	ALDH18A1	NP_002851.2
Pan troglodytes (chimpanzee)	ALDH18A1	XP_001156006.3
Bos taurus (cattle)	ALDH18A1	NP_001039646.1
Danio rerio (zebrafish)	LOC100332705	XP_005156399.1
Oryza sativa (rice)	Os01g0848200	NP_001044802.1
Caenorhabditis elegans (roundworm)	alh-13	NP_510132.1
Oryza sativa (rice)	Os05g0455500	NP_001055723.1
Gallus gallus (chicken)	ALDH18A1	XP_004942438.1
Drosophila melanogaster (fruit fly)	CG7470	NP_001246877.1
	ALDH18A1	XP_001099461.2
Rattus norvegicus (Norway rat)	Aldh18a1	NP_001101994.1
Danio rerio (zebrafish)	aldh18a1	NP_001077015.1
Arabidopsis thaliana (thale cress)	P5CS2	NP_191120.2
Xenopus tropicalis (tropical clawed frog)	aldh18a1	NP_001017306.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Lin X, Kaul S, Rounsley S, Shea TP, Benito MI, Town CD, Fujii CY, Mason T, Bowman CL, Barnstead M, Feldblyum TV, Buell CR, Ketchum KA, Lee J, Ronning CM, Koo HL, Moffat KS, Cronin LA, Shen M, Pai G, Van Aken S, Umayam L, Tallon LJ, Gill JE, Adams MD, Carrera AJ, Creasy TH, Goodman HM, Somerville CR, Copenhaver GP, Preuss D, Nierman WC, White O, Eisen JA, Salzberg SL, Fraser CM, Venter JC
Nature402(6763)761-8(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Taken together, our findings reveal the novel function of FRI in enhancing drought resistance through its downstream P5CS1 pathway during water-deficit stress, which is dependent on its target, the FLC gene.
Title: Functional FRIGIDA allele enhances drought tolerance by regulating the P5CS1 pathway in Arabidopsis thaliana.

Evaluation of Salt Tolerance of Transgenic Tobacco Plants Bearing with P5CS1 Gene of Arabidopsis thaliana
Title: [Evaluation of Salt Tolerance of Transgenic Tobacco Plants Bearing with P5CS1 Gene of Arabidopsis thaliana].

Intron-mediated alternative splicing of Arabidopsis P5CS1 and its association with natural variation in proline and climate adaptation.
Title: Intron-mediated alternative splicing of Arabidopsis P5CS1 and its association with natural variation in proline and climate adaptation.

P5CS1 and P5CS2 are expressed at similar levels and with the same pattern of expression in vegetative and floral shoot apical meristems as well as in axillary meristems.
Title: The proline biosynthetic genes P5CS1 and P5CS2 play overlapping roles in Arabidopsis flower transition but not in embryo development.

Results show that down-regulation of P5CS1 occurred in 35S-P5CS1 leaves, leading to proline reduction, and, in turn, impaired bolting and stunted growth.
Title: Modulation of intracellular proline levels affects flowering time and inflorescence architecture in Arabidopsis.

The following P5CS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the P5CS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb09267	NM_201912.1 Latest version!	Arabidopsis thaliana delta1-pyrroline-5-carboxylate synthase 1 (P5CS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb09266	NM_129539.2 Latest version!	Arabidopsis thaliana delta1-pyrroline-5-carboxylate synthase 1 (P5CS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb09266	NM_001202785.1 Latest version!	Arabidopsis thaliana delta1-pyrroline-5-carboxylate synthase 1 (P5CS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb30108	NM_001202786.1 Latest version!	Arabidopsis thaliana delta1-pyrroline-5-carboxylate synthase 1 (P5CS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

P5CS1 ( NM_201912.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_973641.1 Arabidopsis thaliana delta1-pyrroline-5-carboxylate synthase 1 (P5CS1), mRNA.

Price & Availability↑

CloneID	OAb09267
Clone ID Related Accession (Same CDS sequence)	NM_201912.1
Accession Version	NM_201912.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	delta1-pyrroline-5-carboxylate synthase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071).

ATGGCTTACT ATGAGACTAT GTTTGACCAG CTTGATGTGA CGGCAGCTCA ACTTCTGGTG 
AATGACAGTA GTTTTAGAGA CAAGGATTTC AGGAAGCAAC TTAATGAAAC TGTCAAGTCT 
ATGCTTGATT TGAGGGTTAT TCCAATTTTC AATGAGAATG ATGCTATTAG CACCCGAAGA 
GCCCCATATC AGGATTCTTC TGGTATTTTC TGGGATAACG ATAGCTTAGC TGCTCTACTG 
GCGTTGGAAC TGAAAGCTGA TCTTCTGATT CTTCTGAGCG ATGTTGAAGG TCTTTACACA 
GGCCCTCCAA GTGATCCTAA CTCAAAGTTG ATCCACACTT TTGTTAAAGA AAAACATCAA 
GATGAGATTA CATTCGGCGA CAAATCAAGA TTAGGGAGAG GGGGTATGAC TGCAAAAGTC 
AAAGCTGCAG TCAATGCAGC TTATGCTGGG ATTCCTGTCA TCATAACCAG TGGGTATTCA 
GCTGAGAACA TAGATAAAGT CCTCAGAGGA CTACGTGTTG GAACCTTGTT TCATCAAGAT 
GCTCGTTTAT GGGCTCCGAT CACAGATTCT AATGCTCGTG ACATGGCAGT TGCTGCGAGG 
GAAAGTTCCA GAAAGCTTCA GGCCTTATCT TCGGAAGACA GGAAAAAAAT TCTGCTTGAT 
ATTGCCGATG CCCTTGAAGC AAATGTTACT ACAATCAAAG CTGAGAATGA GTTAGATGTA 
GCTTCTGCAC AAGAGGCTGG GTTGGAAGAG TCAATGGTGG CTCGCTTAGT TATGACACCT 
GGAAAGATCT CGAGCCTTGC AGCTTCAGTT CGTAAGCTAG CTGATATGGA AGATCCAATC 
GGCCGTGTTT TAAAGAAAAC AGAGGTGGCA GATGGTCTTG TCTTAGAGAA GACCTCATCA 
CCATTAGGCG TACTTCTGAT TGTTTTTGAA TCCCGACCTG ATGCACTTGT ACAGATAGCT 
TCACTTGCCA TCCGTAGTGG AAATGGTCTT CTGCTGAAGG GTGGAAAGGA GGCCCGGCGA 
TCAAATGCTA TCTTACACAA GGTGATCACT GATGCAATTC CAGAGACTGT TGGGGGTAAA 
CTCATTGGAC TTGTGACTTC AAGAGAAGAG ATTCCTGATT TGCTTAAGCT TGATGACGTT 
ATCGATCTTG TGATCCCAAG AGGAAGCAAC AAGCTTGTTA CTCAGATAAA AAATACTACA 
AAAATCCCTG TGCTAGGTCA TGCTGATGGA ATCTGTCATG TATATGTCGA CAAGGCTTGT 
GATACGGATA TGGCAAAGCG CATAGTTTCT GATGCAAAGT TGGACTATCC AGCAGCCTGT 
AATGCGATGG AAACCCTTCT TGTGCATAAG GATCTAGAGC AGAATGCTGT GCTTAATGAG 
CTTATTTTTG CTCTGCAGAG CAATGGAGTC ACTTTGTATG GTGGACCAAG GGCAAGTAAG 
ATACTGAACA TACCAGAAGC ACGGTCATTC AACCATGAGT ACTGTGCCAA GGCTTGCACT 
GTTGAAGTTG TAGAAGACGT TTATGGTGCT ATAGATCACA TTCACCGACA TGGGAGTGCA 
CACACAGACT GCATTGTGAC AGAGGATCAC GAAGTTGCAG AGCTATTCCT TCGCCAAGTG 
GATAGCGCTG CTGTGTTCCA CAACGCCAGC ACAAGATTCT CAGATGGTTT CCGATTTGGA 
CTTGGTGCAG AGGTGGGGGT AAGCACGGGC AGGATCCATG CTCGTGGTCC AGTCGGGGTC 
GAAGGATTAC TTACAACGAG ATGGATAATG AGAGGAAAAG GACAAGTTGT CGACGGAGAC 
AATGGAATTG TTTACACCCA TCAGGACATT CCCATCCAAG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_973641.1
CDS	326..2170
Translation	MAYYETMFDQLDVTAAQLLVNDSSFRDKDFRKQLNETVKSMLDLRVIPIFNENDAISTRRAPYQDSSGIFWDNDSLAALLALELKADLLILLSDVEGLYTGPPSDPNSKLIHTFVKEKHQDEITFGDKSRLGRGGMTAKVKAAVNAAYAGIPVIITSGYSAENIDKVLRGLRVGTLFHQDARLWAPITDSNARDMAVAARESSRKLQALSSEDRKKILLDIADALEANVTTIKAENELDVASAQEAGLEESMVARLVMTPGKISSLAASVRKLADMEDPIGRVLKKTEVADGLVLEKTSSPLGVLLIVFESRPDALVQIASLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVTQIKNTTKIPVLGHADGICHVYVDKACDTDMAKRIVSDAKLDYPAACNAMETLLVHKDLEQNAVLNELIFALQSNGVTLYGGPRASKILNIPEARSFNHEYCAKACTVEVVEDVYGAIDHIHRHGSAHTDCIVTEDHEVAELFLRQVDSAAVFHNASTRFSDGFRFGLGAEVGVSTGRIHARGPVGVEGLLTTRWIMRGKGQVVDGDNGIVYTHQDIPIQA

Target ORF information:

RefSeq Version	NM_201912.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana delta1-pyrroline-5-carboxylate synthase 1 (P5CS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201912.1

ORF Insert Sequence:

ATGGCTTACT ATGAGACTAT GTTTGACCAG CTTGATGTGA CGGCAGCTCA ACTTCTGGTG 
AATGACAGTA GTTTTAGAGA CAAGGATTTC AGGAAGCAAC TTAATGAAAC TGTCAAGTCT 
ATGCTTGATT TGAGGGTTAT TCCAATTTTC AATGAGAATG ATGCTATTAG CACCCGAAGA 
GCCCCATATC AGGATTCTTC TGGTATTTTC TGGGATAACG ATAGCTTAGC TGCTCTACTG 
GCGTTGGAAC TGAAAGCTGA TCTTCTGATT CTTCTGAGCG ATGTTGAAGG TCTTTACACA 
GGCCCTCCAA GTGATCCTAA CTCAAAGTTG ATCCACACTT TTGTTAAAGA AAAACATCAA 
GATGAGATTA CATTCGGCGA CAAATCAAGA TTAGGGAGAG GGGGTATGAC TGCAAAAGTC 
AAAGCTGCAG TCAATGCAGC TTATGCTGGG ATTCCTGTCA TCATAACCAG TGGGTATTCA 
GCTGAGAACA TAGATAAAGT CCTCAGAGGA CTACGTGTTG GAACCTTGTT TCATCAAGAT 
GCTCGTTTAT GGGCTCCGAT CACAGATTCT AATGCTCGTG ACATGGCAGT TGCTGCGAGG 
GAAAGTTCCA GAAAGCTTCA GGCCTTATCT TCGGAAGACA GGAAAAAAAT TCTGCTTGAT 
ATTGCCGATG CCCTTGAAGC AAATGTTACT ACAATCAAAG CTGAGAATGA GTTAGATGTA 
GCTTCTGCAC AAGAGGCTGG GTTGGAAGAG TCAATGGTGG CTCGCTTAGT TATGACACCT 
GGAAAGATCT CGAGCCTTGC AGCTTCAGTT CGTAAGCTAG CTGATATGGA AGATCCAATC 
GGCCGTGTTT TAAAGAAAAC AGAGGTGGCA GATGGTCTTG TCTTAGAGAA GACCTCATCA 
CCATTAGGCG TACTTCTGAT TGTTTTTGAA TCCCGACCTG ATGCACTTGT ACAGATAGCT 
TCACTTGCCA TCCGTAGTGG AAATGGTCTT CTGCTGAAGG GTGGAAAGGA GGCCCGGCGA 
TCAAATGCTA TCTTACACAA GGTGATCACT GATGCAATTC CAGAGACTGT TGGGGGTAAA 
CTCATTGGAC TTGTGACTTC AAGAGAAGAG ATTCCTGATT TGCTTAAGCT TGATGACGTT 
ATCGATCTTG TGATCCCAAG AGGAAGCAAC AAGCTTGTTA CTCAGATAAA AAATACTACA 
AAAATCCCTG TGCTAGGTCA TGCTGATGGA ATCTGTCATG TATATGTCGA CAAGGCTTGT 
GATACGGATA TGGCAAAGCG CATAGTTTCT GATGCAAAGT TGGACTATCC AGCAGCCTGT 
AATGCGATGG AAACCCTTCT TGTGCATAAG GATCTAGAGC AGAATGCTGT GCTTAATGAG 
CTTATTTTTG CTCTGCAGAG CAATGGAGTC ACTTTGTATG GTGGACCAAG GGCAAGTAAG 
ATACTGAACA TACCAGAAGC ACGGTCATTC AACCATGAGT ACTGTGCCAA GGCTTGCACT 
GTTGAAGTTG TAGAAGACGT TTATGGTGCT ATAGATCACA TTCACCGACA TGGGAGTGCA 
CACACAGACT GCATTGTGAC AGAGGATCAC GAAGTTGCAG AGCTATTCCT TCGCCAAGTG 
GATAGCGCTG CTGTGTTCCA CAACGCCAGC ACAAGATTCT CAGATGGTTT CCGATTTGGA 
CTTGGTGCAG AGGTGGGGGT AAGCACGGGC AGGATCCATG CTCGTGGTCC AGTCGGGGTC 
GAAGGATTAC TTACAACGAG ATGGATAATG AGAGGAAAAG GACAAGTTGT CGACGGAGAC 
AATGGAATTG TTTACACCCA TCAGGACATT CCCATCCAAG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P5CS1 ( NM_129539.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_181510.1 Arabidopsis thaliana delta1-pyrroline-5-carboxylate synthase 1 (P5CS1), mRNA.

Price & Availability↑

CloneID	OAb09266
Clone ID Related Accession (Same CDS sequence)	NM_129539.2 , NM_001202785.1
Accession Version	NM_129539.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	delta1-pyrroline-5-carboxylate synthase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). On May 13, 2003 this sequence version replaced NM_129539.1.

ATGGAGGAGC TAGATCGTTC ACGTGCTTTT GCCAGAGACG TCAAACGTAT CGTCGTTAAG 
GTTGGGACAG CAGTTGTTAC TGGAAAAGGT GGAAGATTGG CTCTTGGTCG TTTAGGAGCA 
CTGTGTGAAC AGCTTGCGGA ATTAAACTCG GATGGATTTG AGGTGATATT GGTGTCATCT 
GGTGCGGTTG GTCTTGGCAG GCAAAGGCTT CGTTATCGAC AATTAGTCAA TAGCAGCTTT 
GCGGATCTTC AGAAGCCTCA GACTGAACTT GATGGGAAGG CTTGTGCTGG TGTTGGACAA 
AGCAGTCTTA TGGCTTACTA TGAGACTATG TTTGACCAGC TTGATGTGAC GGCAGCTCAA 
CTTCTGGTGA ATGACAGTAG TTTTAGAGAC AAGGATTTCA GGAAGCAACT TAATGAAACT 
GTCAAGTCTA TGCTTGATTT GAGGGTTATT CCAATTTTCA ATGAGAATGA TGCTATTAGC 
ACCCGAAGAG CCCCATATCA GGATTCTTCT GGTATTTTCT GGGATAACGA TAGCTTAGCT 
GCTCTACTGG CGTTGGAACT GAAAGCTGAT CTTCTGATTC TTCTGAGCGA TGTTGAAGGT 
CTTTACACAG GCCCTCCAAG TGATCCTAAC TCAAAGTTGA TCCACACTTT TGTTAAAGAA 
AAACATCAAG ATGAGATTAC ATTCGGCGAC AAATCAAGAT TAGGGAGAGG GGGTATGACT 
GCAAAAGTCA AAGCTGCAGT CAATGCAGCT TATGCTGGGA TTCCTGTCAT CATAACCAGT 
GGGTATTCAG CTGAGAACAT AGATAAAGTC CTCAGAGGAC TACGTGTTGG AACCTTGTTT 
CATCAAGATG CTCGTTTATG GGCTCCGATC ACAGATTCTA ATGCTCGTGA CATGGCAGTT 
GCTGCGAGGG AAAGTTCCAG AAAGCTTCAG GCCTTATCTT CGGAAGACAG GAAAAAAATT 
CTGCTTGATA TTGCCGATGC CCTTGAAGCA AATGTTACTA CAATCAAAGC TGAGAATGAG 
TTAGATGTAG CTTCTGCACA AGAGGCTGGG TTGGAAGAGT CAATGGTGGC TCGCTTAGTT 
ATGACACCTG GAAAGATCTC GAGCCTTGCA GCTTCAGTTC GTAAGCTAGC TGATATGGAA 
GATCCAATCG GCCGTGTTTT AAAGAAAACA GAGGTGGCAG ATGGTCTTGT CTTAGAGAAG 
ACCTCATCAC CATTAGGCGT ACTTCTGATT GTTTTTGAAT CCCGACCTGA TGCACTTGTA 
CAGATAGCTT CACTTGCCAT CCGTAGTGGA AATGGTCTTC TGCTGAAGGG TGGAAAGGAG 
GCCCGGCGAT CAAATGCTAT CTTACACAAG GTGATCACTG ATGCAATTCC AGAGACTGTT 
GGGGGTAAAC TCATTGGACT TGTGACTTCA AGAGAAGAGA TTCCTGATTT GCTTAAGCTT 
GATGACGTTA TCGATCTTGT GATCCCAAGA GGAAGCAACA AGCTTGTTAC TCAGATAAAA 
AATACTACAA AAATCCCTGT GCTAGGTCAT GCTGATGGAA TCTGTCATGT ATATGTCGAC 
AAGGCTTGTG ATACGGATAT GGCAAAGCGC ATAGTTTCTG ATGCAAAGTT GGACTATCCA 
GCAGCCTGTA ATGCGATGGA AACCCTTCTT GTGCATAAGG ATCTAGAGCA GAATGCTGTG 
CTTAATGAGC TTATTTTTGC TCTGCAGAGC AATGGAGTCA CTTTGTATGG TGGACCAAGG 
GCAAGTAAGA TACTGAACAT ACCAGAAGCA CGGTCATTCA ACCATGAGTA CTGTGCCAAG 
GCTTGCACTG TTGAAGTTGT AGAAGACGTT TATGGTGCTA TAGATCACAT TCACCGACAT 
GGGAGTGCAC ACACAGACTG CATTGTGACA GAGGATCACG AAGTTGCAGA GCTATTCCTT 
CGCCAAGTGG ATAGCGCTGC TGTGTTCCAC AACGCCAGCA CAAGATTCTC AGATGGTTTC 
CGATTTGGAC TTGGTGCAGA GGTGGGGGTA AGCACGGGCA GGATCCATGC TCGTGGTCCA 
GTCGGGGTCG AAGGATTACT TACAACGAGA TGGATAATGA GAGGAAAAGG ACAAGTTGTC 
GACGGAGACA ATGGAATTGT TTACACCCAT CAGGACATTC CCATCCAAGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_181510.1
CDS	381..2534
Translation	MEELDRSRAFARDVKRIVVKVGTAVVTGKGGRLALGRLGALCEQLAELNSDGFEVILVSSGAVGLGRQRLRYRQLVNSSFADLQKPQTELDGKACAGVGQSSLMAYYETMFDQLDVTAAQLLVNDSSFRDKDFRKQLNETVKSMLDLRVIPIFNENDAISTRRAPYQDSSGIFWDNDSLAALLALELKADLLILLSDVEGLYTGPPSDPNSKLIHTFVKEKHQDEITFGDKSRLGRGGMTAKVKAAVNAAYAGIPVIITSGYSAENIDKVLRGLRVGTLFHQDARLWAPITDSNARDMAVAARESSRKLQALSSEDRKKILLDIADALEANVTTIKAENELDVASAQEAGLEESMVARLVMTPGKISSLAASVRKLADMEDPIGRVLKKTEVADGLVLEKTSSPLGVLLIVFESRPDALVQIASLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVTQIKNTTKIPVLGHADGICHVYVDKACDTDMAKRIVSDAKLDYPAACNAMETLLVHKDLEQNAVLNELIFALQSNGVTLYGGPRASKILNIPEARSFNHEYCAKACTVEVVEDVYGAIDHIHRHGSAHTDCIVTEDHEVAELFLRQVDSAAVFHNASTRFSDGFRFGLGAEVGVSTGRIHARGPVGVEGLLTTRWIMRGKGQVVDGDNGIVYTHQDIPIQA

Target ORF information:

RefSeq Version	NM_129539.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana delta1-pyrroline-5-carboxylate synthase 1 (P5CS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_129539.2

ORF Insert Sequence:

ATGGAGGAGC TAGATCGTTC ACGTGCTTTT GCCAGAGACG TCAAACGTAT CGTCGTTAAG 
GTTGGGACAG CAGTTGTTAC TGGAAAAGGT GGAAGATTGG CTCTTGGTCG TTTAGGAGCA 
CTGTGTGAAC AGCTTGCGGA ATTAAACTCG GATGGATTTG AGGTGATATT GGTGTCATCT 
GGTGCGGTTG GTCTTGGCAG GCAAAGGCTT CGTTATCGAC AATTAGTCAA TAGCAGCTTT 
GCGGATCTTC AGAAGCCTCA GACTGAACTT GATGGGAAGG CTTGTGCTGG TGTTGGACAA 
AGCAGTCTTA TGGCTTACTA TGAGACTATG TTTGACCAGC TTGATGTGAC GGCAGCTCAA 
CTTCTGGTGA ATGACAGTAG TTTTAGAGAC AAGGATTTCA GGAAGCAACT TAATGAAACT 
GTCAAGTCTA TGCTTGATTT GAGGGTTATT CCAATTTTCA ATGAGAATGA TGCTATTAGC 
ACCCGAAGAG CCCCATATCA GGATTCTTCT GGTATTTTCT GGGATAACGA TAGCTTAGCT 
GCTCTACTGG CGTTGGAACT GAAAGCTGAT CTTCTGATTC TTCTGAGCGA TGTTGAAGGT 
CTTTACACAG GCCCTCCAAG TGATCCTAAC TCAAAGTTGA TCCACACTTT TGTTAAAGAA 
AAACATCAAG ATGAGATTAC ATTCGGCGAC AAATCAAGAT TAGGGAGAGG GGGTATGACT 
GCAAAAGTCA AAGCTGCAGT CAATGCAGCT TATGCTGGGA TTCCTGTCAT CATAACCAGT 
GGGTATTCAG CTGAGAACAT AGATAAAGTC CTCAGAGGAC TACGTGTTGG AACCTTGTTT 
CATCAAGATG CTCGTTTATG GGCTCCGATC ACAGATTCTA ATGCTCGTGA CATGGCAGTT 
GCTGCGAGGG AAAGTTCCAG AAAGCTTCAG GCCTTATCTT CGGAAGACAG GAAAAAAATT 
CTGCTTGATA TTGCCGATGC CCTTGAAGCA AATGTTACTA CAATCAAAGC TGAGAATGAG 
TTAGATGTAG CTTCTGCACA AGAGGCTGGG TTGGAAGAGT CAATGGTGGC TCGCTTAGTT 
ATGACACCTG GAAAGATCTC GAGCCTTGCA GCTTCAGTTC GTAAGCTAGC TGATATGGAA 
GATCCAATCG GCCGTGTTTT AAAGAAAACA GAGGTGGCAG ATGGTCTTGT CTTAGAGAAG 
ACCTCATCAC CATTAGGCGT ACTTCTGATT GTTTTTGAAT CCCGACCTGA TGCACTTGTA 
CAGATAGCTT CACTTGCCAT CCGTAGTGGA AATGGTCTTC TGCTGAAGGG TGGAAAGGAG 
GCCCGGCGAT CAAATGCTAT CTTACACAAG GTGATCACTG ATGCAATTCC AGAGACTGTT 
GGGGGTAAAC TCATTGGACT TGTGACTTCA AGAGAAGAGA TTCCTGATTT GCTTAAGCTT 
GATGACGTTA TCGATCTTGT GATCCCAAGA GGAAGCAACA AGCTTGTTAC TCAGATAAAA 
AATACTACAA AAATCCCTGT GCTAGGTCAT GCTGATGGAA TCTGTCATGT ATATGTCGAC 
AAGGCTTGTG ATACGGATAT GGCAAAGCGC ATAGTTTCTG ATGCAAAGTT GGACTATCCA 
GCAGCCTGTA ATGCGATGGA AACCCTTCTT GTGCATAAGG ATCTAGAGCA GAATGCTGTG 
CTTAATGAGC TTATTTTTGC TCTGCAGAGC AATGGAGTCA CTTTGTATGG TGGACCAAGG 
GCAAGTAAGA TACTGAACAT ACCAGAAGCA CGGTCATTCA ACCATGAGTA CTGTGCCAAG 
GCTTGCACTG TTGAAGTTGT AGAAGACGTT TATGGTGCTA TAGATCACAT TCACCGACAT 
GGGAGTGCAC ACACAGACTG CATTGTGACA GAGGATCACG AAGTTGCAGA GCTATTCCTT 
CGCCAAGTGG ATAGCGCTGC TGTGTTCCAC AACGCCAGCA CAAGATTCTC AGATGGTTTC 
CGATTTGGAC TTGGTGCAGA GGTGGGGGTA AGCACGGGCA GGATCCATGC TCGTGGTCCA 
GTCGGGGTCG AAGGATTACT TACAACGAGA TGGATAATGA GAGGAAAAGG ACAAGTTGTC 
GACGGAGACA ATGGAATTGT TTACACCCAT CAGGACATTC CCATCCAAGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P5CS1 ( NM_001202785.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001189714.1 Arabidopsis thaliana delta1-pyrroline-5-carboxylate synthase 1 (P5CS1), mRNA.

Price & Availability↑

CloneID	OAb09266
Clone ID Related Accession (Same CDS sequence)	NM_129539.2 , NM_001202785.1
Accession Version	NM_001202785.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	delta1-pyrroline-5-carboxylate synthase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071).

ATGGAGGAGC TAGATCGTTC ACGTGCTTTT GCCAGAGACG TCAAACGTAT CGTCGTTAAG 
GTTGGGACAG CAGTTGTTAC TGGAAAAGGT GGAAGATTGG CTCTTGGTCG TTTAGGAGCA 
CTGTGTGAAC AGCTTGCGGA ATTAAACTCG GATGGATTTG AGGTGATATT GGTGTCATCT 
GGTGCGGTTG GTCTTGGCAG GCAAAGGCTT CGTTATCGAC AATTAGTCAA TAGCAGCTTT 
GCGGATCTTC AGAAGCCTCA GACTGAACTT GATGGGAAGG CTTGTGCTGG TGTTGGACAA 
AGCAGTCTTA TGGCTTACTA TGAGACTATG TTTGACCAGC TTGATGTGAC GGCAGCTCAA 
CTTCTGGTGA ATGACAGTAG TTTTAGAGAC AAGGATTTCA GGAAGCAACT TAATGAAACT 
GTCAAGTCTA TGCTTGATTT GAGGGTTATT CCAATTTTCA ATGAGAATGA TGCTATTAGC 
ACCCGAAGAG CCCCATATCA GGATTCTTCT GGTATTTTCT GGGATAACGA TAGCTTAGCT 
GCTCTACTGG CGTTGGAACT GAAAGCTGAT CTTCTGATTC TTCTGAGCGA TGTTGAAGGT 
CTTTACACAG GCCCTCCAAG TGATCCTAAC TCAAAGTTGA TCCACACTTT TGTTAAAGAA 
AAACATCAAG ATGAGATTAC ATTCGGCGAC AAATCAAGAT TAGGGAGAGG GGGTATGACT 
GCAAAAGTCA AAGCTGCAGT CAATGCAGCT TATGCTGGGA TTCCTGTCAT CATAACCAGT 
GGGTATTCAG CTGAGAACAT AGATAAAGTC CTCAGAGGAC TACGTGTTGG AACCTTGTTT 
CATCAAGATG CTCGTTTATG GGCTCCGATC ACAGATTCTA ATGCTCGTGA CATGGCAGTT 
GCTGCGAGGG AAAGTTCCAG AAAGCTTCAG GCCTTATCTT CGGAAGACAG GAAAAAAATT 
CTGCTTGATA TTGCCGATGC CCTTGAAGCA AATGTTACTA CAATCAAAGC TGAGAATGAG 
TTAGATGTAG CTTCTGCACA AGAGGCTGGG TTGGAAGAGT CAATGGTGGC TCGCTTAGTT 
ATGACACCTG GAAAGATCTC GAGCCTTGCA GCTTCAGTTC GTAAGCTAGC TGATATGGAA 
GATCCAATCG GCCGTGTTTT AAAGAAAACA GAGGTGGCAG ATGGTCTTGT CTTAGAGAAG 
ACCTCATCAC CATTAGGCGT ACTTCTGATT GTTTTTGAAT CCCGACCTGA TGCACTTGTA 
CAGATAGCTT CACTTGCCAT CCGTAGTGGA AATGGTCTTC TGCTGAAGGG TGGAAAGGAG 
GCCCGGCGAT CAAATGCTAT CTTACACAAG GTGATCACTG ATGCAATTCC AGAGACTGTT 
GGGGGTAAAC TCATTGGACT TGTGACTTCA AGAGAAGAGA TTCCTGATTT GCTTAAGCTT 
GATGACGTTA TCGATCTTGT GATCCCAAGA GGAAGCAACA AGCTTGTTAC TCAGATAAAA 
AATACTACAA AAATCCCTGT GCTAGGTCAT GCTGATGGAA TCTGTCATGT ATATGTCGAC 
AAGGCTTGTG ATACGGATAT GGCAAAGCGC ATAGTTTCTG ATGCAAAGTT GGACTATCCA 
GCAGCCTGTA ATGCGATGGA AACCCTTCTT GTGCATAAGG ATCTAGAGCA GAATGCTGTG 
CTTAATGAGC TTATTTTTGC TCTGCAGAGC AATGGAGTCA CTTTGTATGG TGGACCAAGG 
GCAAGTAAGA TACTGAACAT ACCAGAAGCA CGGTCATTCA ACCATGAGTA CTGTGCCAAG 
GCTTGCACTG TTGAAGTTGT AGAAGACGTT TATGGTGCTA TAGATCACAT TCACCGACAT 
GGGAGTGCAC ACACAGACTG CATTGTGACA GAGGATCACG AAGTTGCAGA GCTATTCCTT 
CGCCAAGTGG ATAGCGCTGC TGTGTTCCAC AACGCCAGCA CAAGATTCTC AGATGGTTTC 
CGATTTGGAC TTGGTGCAGA GGTGGGGGTA AGCACGGGCA GGATCCATGC TCGTGGTCCA 
GTCGGGGTCG AAGGATTACT TACAACGAGA TGGATAATGA GAGGAAAAGG ACAAGTTGTC 
GACGGAGACA ATGGAATTGT TTACACCCAT CAGGACATTC CCATCCAAGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189714.1
CDS	123..2276
Translation	MEELDRSRAFARDVKRIVVKVGTAVVTGKGGRLALGRLGALCEQLAELNSDGFEVILVSSGAVGLGRQRLRYRQLVNSSFADLQKPQTELDGKACAGVGQSSLMAYYETMFDQLDVTAAQLLVNDSSFRDKDFRKQLNETVKSMLDLRVIPIFNENDAISTRRAPYQDSSGIFWDNDSLAALLALELKADLLILLSDVEGLYTGPPSDPNSKLIHTFVKEKHQDEITFGDKSRLGRGGMTAKVKAAVNAAYAGIPVIITSGYSAENIDKVLRGLRVGTLFHQDARLWAPITDSNARDMAVAARESSRKLQALSSEDRKKILLDIADALEANVTTIKAENELDVASAQEAGLEESMVARLVMTPGKISSLAASVRKLADMEDPIGRVLKKTEVADGLVLEKTSSPLGVLLIVFESRPDALVQIASLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVTQIKNTTKIPVLGHADGICHVYVDKACDTDMAKRIVSDAKLDYPAACNAMETLLVHKDLEQNAVLNELIFALQSNGVTLYGGPRASKILNIPEARSFNHEYCAKACTVEVVEDVYGAIDHIHRHGSAHTDCIVTEDHEVAELFLRQVDSAAVFHNASTRFSDGFRFGLGAEVGVSTGRIHARGPVGVEGLLTTRWIMRGKGQVVDGDNGIVYTHQDIPIQA

Target ORF information:

RefSeq Version	NM_001202785.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana delta1-pyrroline-5-carboxylate synthase 1 (P5CS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202785.1

ORF Insert Sequence:

ATGGAGGAGC TAGATCGTTC ACGTGCTTTT GCCAGAGACG TCAAACGTAT CGTCGTTAAG 
GTTGGGACAG CAGTTGTTAC TGGAAAAGGT GGAAGATTGG CTCTTGGTCG TTTAGGAGCA 
CTGTGTGAAC AGCTTGCGGA ATTAAACTCG GATGGATTTG AGGTGATATT GGTGTCATCT 
GGTGCGGTTG GTCTTGGCAG GCAAAGGCTT CGTTATCGAC AATTAGTCAA TAGCAGCTTT 
GCGGATCTTC AGAAGCCTCA GACTGAACTT GATGGGAAGG CTTGTGCTGG TGTTGGACAA 
AGCAGTCTTA TGGCTTACTA TGAGACTATG TTTGACCAGC TTGATGTGAC GGCAGCTCAA 
CTTCTGGTGA ATGACAGTAG TTTTAGAGAC AAGGATTTCA GGAAGCAACT TAATGAAACT 
GTCAAGTCTA TGCTTGATTT GAGGGTTATT CCAATTTTCA ATGAGAATGA TGCTATTAGC 
ACCCGAAGAG CCCCATATCA GGATTCTTCT GGTATTTTCT GGGATAACGA TAGCTTAGCT 
GCTCTACTGG CGTTGGAACT GAAAGCTGAT CTTCTGATTC TTCTGAGCGA TGTTGAAGGT 
CTTTACACAG GCCCTCCAAG TGATCCTAAC TCAAAGTTGA TCCACACTTT TGTTAAAGAA 
AAACATCAAG ATGAGATTAC ATTCGGCGAC AAATCAAGAT TAGGGAGAGG GGGTATGACT 
GCAAAAGTCA AAGCTGCAGT CAATGCAGCT TATGCTGGGA TTCCTGTCAT CATAACCAGT 
GGGTATTCAG CTGAGAACAT AGATAAAGTC CTCAGAGGAC TACGTGTTGG AACCTTGTTT 
CATCAAGATG CTCGTTTATG GGCTCCGATC ACAGATTCTA ATGCTCGTGA CATGGCAGTT 
GCTGCGAGGG AAAGTTCCAG AAAGCTTCAG GCCTTATCTT CGGAAGACAG GAAAAAAATT 
CTGCTTGATA TTGCCGATGC CCTTGAAGCA AATGTTACTA CAATCAAAGC TGAGAATGAG 
TTAGATGTAG CTTCTGCACA AGAGGCTGGG TTGGAAGAGT CAATGGTGGC TCGCTTAGTT 
ATGACACCTG GAAAGATCTC GAGCCTTGCA GCTTCAGTTC GTAAGCTAGC TGATATGGAA 
GATCCAATCG GCCGTGTTTT AAAGAAAACA GAGGTGGCAG ATGGTCTTGT CTTAGAGAAG 
ACCTCATCAC CATTAGGCGT ACTTCTGATT GTTTTTGAAT CCCGACCTGA TGCACTTGTA 
CAGATAGCTT CACTTGCCAT CCGTAGTGGA AATGGTCTTC TGCTGAAGGG TGGAAAGGAG 
GCCCGGCGAT CAAATGCTAT CTTACACAAG GTGATCACTG ATGCAATTCC AGAGACTGTT 
GGGGGTAAAC TCATTGGACT TGTGACTTCA AGAGAAGAGA TTCCTGATTT GCTTAAGCTT 
GATGACGTTA TCGATCTTGT GATCCCAAGA GGAAGCAACA AGCTTGTTAC TCAGATAAAA 
AATACTACAA AAATCCCTGT GCTAGGTCAT GCTGATGGAA TCTGTCATGT ATATGTCGAC 
AAGGCTTGTG ATACGGATAT GGCAAAGCGC ATAGTTTCTG ATGCAAAGTT GGACTATCCA 
GCAGCCTGTA ATGCGATGGA AACCCTTCTT GTGCATAAGG ATCTAGAGCA GAATGCTGTG 
CTTAATGAGC TTATTTTTGC TCTGCAGAGC AATGGAGTCA CTTTGTATGG TGGACCAAGG 
GCAAGTAAGA TACTGAACAT ACCAGAAGCA CGGTCATTCA ACCATGAGTA CTGTGCCAAG 
GCTTGCACTG TTGAAGTTGT AGAAGACGTT TATGGTGCTA TAGATCACAT TCACCGACAT 
GGGAGTGCAC ACACAGACTG CATTGTGACA GAGGATCACG AAGTTGCAGA GCTATTCCTT 
CGCCAAGTGG ATAGCGCTGC TGTGTTCCAC AACGCCAGCA CAAGATTCTC AGATGGTTTC 
CGATTTGGAC TTGGTGCAGA GGTGGGGGTA AGCACGGGCA GGATCCATGC TCGTGGTCCA 
GTCGGGGTCG AAGGATTACT TACAACGAGA TGGATAATGA GAGGAAAAGG ACAAGTTGTC 
GACGGAGACA ATGGAATTGT TTACACCCAT CAGGACATTC CCATCCAAGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P5CS1 ( NM_001202786.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001189715.1 Arabidopsis thaliana delta1-pyrroline-5-carboxylate synthase 1 (P5CS1), mRNA.

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CloneID	OAb30108
Clone ID Related Accession (Same CDS sequence)	NM_001202786.1
Accession Version	NM_001202786.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	delta1-pyrroline-5-carboxylate synthase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071).

ATGGAGGAGC TAGATCGTTC ACGTGCTTTT GCCAGAGACG TCAAACGTAT CGTCGTTAAG 
GTTGGGACAG CAGTTGTTAC TGGAAAAGGT GGAAGATTGG CTCTTGGTCG TTTAGGAGCA 
CTGTGTGAAC AGCTTGCGGA ATTAAACTCG GATGGATTTG AGGTGATATT GGTGTCATCT 
GGTGCGGTTG GTCTTGGCAG GCAAAGGCTT CGTTATCGAC AATTAGTCAA TAGCAGCTTT 
GCGGATCTTC AGAAGCCTCA GACTGAACTT GATGGGAAGG CTTGTGCTGG TGTTGGACAA 
AGCAGTCTTA TGGCTTACTA TGAGACTATG TTTGACCAGC TTGATGTGAC GGCAGCTCAA 
CTTCTGGTGA ATGACAGTAG TTTTAGAGAC AAGGATTTCA GGAAGCAACT TAATGAAACT 
GTCAAGTCTA TGCTTGATTT GAGGGTTATT CCAATTTTCA ATGAGAATGA TGCTATTAGC 
ACCCGAAGAG CCCCATATCA GGATTCTTCT GGTATTTTCT GGGATAACGA TAGCTTAGCT 
GCTCTACTGG CGTTGGAACT GAAAGCTGAT CTTCTGATTC TTCTGAGCGA TGTTGAAGGT 
CTTTACACAG GCCCTCCAAG TGATCCTAAC TCAAAGTTGA TCCACACTTT TGTTAAAGAA 
AAACATCAAG ATGAGATTAC ATTCGGCGAC AAATCAAGAT TAGGGAGAGG GGGTATGACT 
GCAAAAGTCA AAGCTGCAGT CAATGCAGCT TATGCTGGGA TTCCTGTCAT CATAACCAGT 
GGGTATTCAG CTGAGAACAT AGATAAAGTC CTCAGAGGAC TACGTGTTGG AACCTTGTTT 
CATCAAGATG CTCGTTTATG GGCTCCGATC ACAGATTCTA ATGCTCGTGA CATGGCAGTT 
GCTGCGAGGG AAAGTTCCAG AAAGCTTCAG GCCTTATCTT CGGAAGACAG GAAAAAAATT 
CTGCTTGATA TTGCCGATGC CCTTGAAGCA AATGTTACTA CAATCAAAGC TGAGAATGAG 
TTAGATGTAG CTTCTGCACA AGAGGCTGGG TTGGAAGAGT CAATGGTGGC TCGCTTAGTT 
ATGACACCTG GAAAGATCTC GAGCCTTGCA GCTTCAGTTC GTAAGCTAGC TGATATGGAA 
GATCCAATCG GCCGTGTTTT AAAGAAAACA GAGGTGGCAG ATGGTCTTGT CTTAGAGAAG 
ACCTCATCAC CATTAGGCGT ACTTCTGATT GTTTTTGAAT CCCGACCTGA TGCACTTGTA 
CAGATAGCTT CACTTGCCAT CCGTAGTGGA AATGGTCTTC TGCTGAAGGG TGGAAAGGAG 
GCCCGGCGAT CAAATGCTAT CTTACACAAG GTGATCACTG ATGCAATTCC AGAGACTGTT 
GGGGGTAAAC TCATTGGACT TGTGACTTCA AGAGAAGAGA TTCCTGATTT GCTTAAGCTT 
GATGACGTTA TCGATCTTGT GATCCCAAGA GGAAGCAACA AGCTTGTTAC TCAGATAAAA 
AATACTACAA AAATCCCTGT GCTAGATGGA ATCTGTCATG TATATGTCGA CAAGGCTTGT 
GATACGGATA TGGCAAAGCG CATAGTTTCT GATGCAAAGT TGGACTATCC AGCAGCCTGT 
AATGCGATGG AAACCCTTCT TGTGCATAAG GATCTAGAGC AGAATGCTGT GCTTAATGAG 
CTTATTTTTG CTCTGCAGAG CAATGGAGTC ACTTTGTATG GTGGACCAAG GGCAAGTAAG 
ATACTGAACA TACCAGAAGC ACGGTCATTC AACCATGAGT ACTGTGCCAA GGCTTGCACT 
GTTGAAGTTG TAGAAGACGT TTATGGTGCT ATAGATCACA TTCACCGACA TGGGAGTGCA 
CACACAGACT GCATTGTGAC AGAGGATCAC GAAGTTGCAG AGCTATTCCT TCGCCAAGTG 
GATAGCGCTG CTGTGTTCCA CAACGCCAGC ACAAGATTCT CAGATGGTTT CCGATTTGGA 
CTTGGTGCAG AGGTGGGGGT AAGCACGGGC AGGATCCATG CTCGTGGTCC AGTCGGGGTC 
GAAGGATTAC TTACAACGAG ATGGATAATG AGAGGAAAAG GACAAGTTGT CGACGGAGAC 
AATGGAATTG TTTACACCCA TCAGGACATT CCCATCCAAG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189715.1
CDS	123..2267
Translation	MEELDRSRAFARDVKRIVVKVGTAVVTGKGGRLALGRLGALCEQLAELNSDGFEVILVSSGAVGLGRQRLRYRQLVNSSFADLQKPQTELDGKACAGVGQSSLMAYYETMFDQLDVTAAQLLVNDSSFRDKDFRKQLNETVKSMLDLRVIPIFNENDAISTRRAPYQDSSGIFWDNDSLAALLALELKADLLILLSDVEGLYTGPPSDPNSKLIHTFVKEKHQDEITFGDKSRLGRGGMTAKVKAAVNAAYAGIPVIITSGYSAENIDKVLRGLRVGTLFHQDARLWAPITDSNARDMAVAARESSRKLQALSSEDRKKILLDIADALEANVTTIKAENELDVASAQEAGLEESMVARLVMTPGKISSLAASVRKLADMEDPIGRVLKKTEVADGLVLEKTSSPLGVLLIVFESRPDALVQIASLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVTQIKNTTKIPVLDGICHVYVDKACDTDMAKRIVSDAKLDYPAACNAMETLLVHKDLEQNAVLNELIFALQSNGVTLYGGPRASKILNIPEARSFNHEYCAKACTVEVVEDVYGAIDHIHRHGSAHTDCIVTEDHEVAELFLRQVDSAAVFHNASTRFSDGFRFGLGAEVGVSTGRIHARGPVGVEGLLTTRWIMRGKGQVVDGDNGIVYTHQDIPIQA

Target ORF information:

RefSeq Version	NM_001202786.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana delta1-pyrroline-5-carboxylate synthase 1 (P5CS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202786.1

ORF Insert Sequence:

ATGGAGGAGC TAGATCGTTC ACGTGCTTTT GCCAGAGACG TCAAACGTAT CGTCGTTAAG 
GTTGGGACAG CAGTTGTTAC TGGAAAAGGT GGAAGATTGG CTCTTGGTCG TTTAGGAGCA 
CTGTGTGAAC AGCTTGCGGA ATTAAACTCG GATGGATTTG AGGTGATATT GGTGTCATCT 
GGTGCGGTTG GTCTTGGCAG GCAAAGGCTT CGTTATCGAC AATTAGTCAA TAGCAGCTTT 
GCGGATCTTC AGAAGCCTCA GACTGAACTT GATGGGAAGG CTTGTGCTGG TGTTGGACAA 
AGCAGTCTTA TGGCTTACTA TGAGACTATG TTTGACCAGC TTGATGTGAC GGCAGCTCAA 
CTTCTGGTGA ATGACAGTAG TTTTAGAGAC AAGGATTTCA GGAAGCAACT TAATGAAACT 
GTCAAGTCTA TGCTTGATTT GAGGGTTATT CCAATTTTCA ATGAGAATGA TGCTATTAGC 
ACCCGAAGAG CCCCATATCA GGATTCTTCT GGTATTTTCT GGGATAACGA TAGCTTAGCT 
GCTCTACTGG CGTTGGAACT GAAAGCTGAT CTTCTGATTC TTCTGAGCGA TGTTGAAGGT 
CTTTACACAG GCCCTCCAAG TGATCCTAAC TCAAAGTTGA TCCACACTTT TGTTAAAGAA 
AAACATCAAG ATGAGATTAC ATTCGGCGAC AAATCAAGAT TAGGGAGAGG GGGTATGACT 
GCAAAAGTCA AAGCTGCAGT CAATGCAGCT TATGCTGGGA TTCCTGTCAT CATAACCAGT 
GGGTATTCAG CTGAGAACAT AGATAAAGTC CTCAGAGGAC TACGTGTTGG AACCTTGTTT 
CATCAAGATG CTCGTTTATG GGCTCCGATC ACAGATTCTA ATGCTCGTGA CATGGCAGTT 
GCTGCGAGGG AAAGTTCCAG AAAGCTTCAG GCCTTATCTT CGGAAGACAG GAAAAAAATT 
CTGCTTGATA TTGCCGATGC CCTTGAAGCA AATGTTACTA CAATCAAAGC TGAGAATGAG 
TTAGATGTAG CTTCTGCACA AGAGGCTGGG TTGGAAGAGT CAATGGTGGC TCGCTTAGTT 
ATGACACCTG GAAAGATCTC GAGCCTTGCA GCTTCAGTTC GTAAGCTAGC TGATATGGAA 
GATCCAATCG GCCGTGTTTT AAAGAAAACA GAGGTGGCAG ATGGTCTTGT CTTAGAGAAG 
ACCTCATCAC CATTAGGCGT ACTTCTGATT GTTTTTGAAT CCCGACCTGA TGCACTTGTA 
CAGATAGCTT CACTTGCCAT CCGTAGTGGA AATGGTCTTC TGCTGAAGGG TGGAAAGGAG 
GCCCGGCGAT CAAATGCTAT CTTACACAAG GTGATCACTG ATGCAATTCC AGAGACTGTT 
GGGGGTAAAC TCATTGGACT TGTGACTTCA AGAGAAGAGA TTCCTGATTT GCTTAAGCTT 
GATGACGTTA TCGATCTTGT GATCCCAAGA GGAAGCAACA AGCTTGTTAC TCAGATAAAA 
AATACTACAA AAATCCCTGT GCTAGATGGA ATCTGTCATG TATATGTCGA CAAGGCTTGT 
GATACGGATA TGGCAAAGCG CATAGTTTCT GATGCAAAGT TGGACTATCC AGCAGCCTGT 
AATGCGATGG AAACCCTTCT TGTGCATAAG GATCTAGAGC AGAATGCTGT GCTTAATGAG 
CTTATTTTTG CTCTGCAGAG CAATGGAGTC ACTTTGTATG GTGGACCAAG GGCAAGTAAG 
ATACTGAACA TACCAGAAGC ACGGTCATTC AACCATGAGT ACTGTGCCAA GGCTTGCACT 
GTTGAAGTTG TAGAAGACGT TTATGGTGCT ATAGATCACA TTCACCGACA TGGGAGTGCA 
CACACAGACT GCATTGTGAC AGAGGATCAC GAAGTTGCAG AGCTATTCCT TCGCCAAGTG 
GATAGCGCTG CTGTGTTCCA CAACGCCAGC ACAAGATTCT CAGATGGTTT CCGATTTGGA 
CTTGGTGCAG AGGTGGGGGT AAGCACGGGC AGGATCCATG CTCGTGGTCC AGTCGGGGTC 
GAAGGATTAC TTACAACGAG ATGGATAATG AGAGGAAAAG GACAAGTTGT CGACGGAGAC 
AATGGAATTG TTTACACCCA TCAGGACATT CCCATCCAAG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Nature402(6763)761-8(1999 Dec)
Lin X,Kaul S,Rounsley S,Shea TP,Benito MI,Town CD,Fujii CY,Mason T,Bowman CL,Barnstead M,Feldblyum TV,Buell CR,Ketchum KA,Lee J,Ronning CM,Koo HL,Moffat KS,Cronin LA,Shen M,Pai G,Van Aken S,Umayam L,Tallon LJ,Gill JE,Adams MD,Carrera AJ,Creasy TH,Goodman HM,Somerville CR,Copenhaver GP,Preuss D,Nierman WC,White O,Eisen JA,Salzberg SL,Fraser CM,Venter JC

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P5CS1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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