MEE29 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	MEE29
Entrez Gene ID	818101
Full Name	helicase domain-containing protein
Synonyms	T32F12.28,T32F12_28,maternal effect embryo arrest 29
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_129086.4	NP_181077.3	helicase domain-containing protein
NM_001336552.1	NP_001323587.1	helicase domain-containing protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	DHX16	NP_003578.2
Rattus norvegicus (Norway rat)	Dhx16	NP_997661.1
Caenorhabditis elegans (roundworm)	mog-4	NP_497027.1
Schizosaccharomyces pombe (fission yeast)	cdc28	NP_595686.2
Neurospora crassa	NCU08928	XP_960795.1
Drosophila melanogaster (fruit fly)	l(2)37Cb	NP_609946.1
Pan troglodytes (chimpanzee)	DHX16	NP_001035839.1
	DHX16	NP_001098636.1
Mus musculus (house mouse)	Dhx16	NP_081263.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009093	XP_319843.4
Arabidopsis thaliana (thale cress)	ESP3	NP_174527.2
Canis lupus familiaris (dog)	DHX16	XP_538827.2
Danio rerio (zebrafish)	dhx16	NP_956318.1
Bos taurus (cattle)	DHX16	NP_001193139.1
Magnaporthe oryzae (rice blast fungus)	MGG_07501	XP_003711413.1
Arabidopsis thaliana (thale cress)	MEE29	NP_181077.3
Oryza sativa (rice)	Os05g0389800	NP_001055437.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Lin X, Kaul S, Rounsley S, Shea TP, Benito MI, Town CD, Fujii CY, Mason T, Bowman CL, Barnstead M, Feldblyum TV, Buell CR, Ketchum KA, Lee J, Ronning CM, Koo HL, Moffat KS, Cronin LA, Shen M, Pai G, Van Aken S, Umayam L, Tallon LJ, Gill JE, Adams MD, Carrera AJ, Creasy TH, Goodman HM, Somerville CR, Copenhaver GP, Preuss D, Nierman WC, White O, Eisen JA, Salzberg SL, Fraser CM, Venter JC
Nature402(6763)761-8(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MEE29 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MEE29 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb36324	NM_001336552.1 Latest version!	Arabidopsis thaliana helicase domain-containing protein (MEE29), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAb08823	NM_129086.4 Latest version!	Arabidopsis thaliana helicase domain-containing protein (MEE29), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MEE29 ( NM_001336552.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001323587.1 Arabidopsis thaliana helicase domain-containing protein (MEE29), mRNA.

Price & Availability↑

CloneID	OAb36324
Clone ID Related Accession (Same CDS sequence)	NM_001336552.1
Accession Version	NM_001336552.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	helicase domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071).

ATGCAGGTAG TGATTGTTAG AGAGGACAAA AGGAATGTTA GGAGAAAGGT TTCCGAAGAC 
GAGGATGATG GCACTGAGTC TGAAGAGGAA AGATTACGTG ACCAAAGGGA GAGAGAAGAA 
CTAGAGCAGC ACCTAAGAGA ACGAGATACT GCACGGACAC GAAAGCTAAC CGAGCCAAAA 
ATGTCAAAGA AAGAGCAAGA AGAGTTTGTT CGAAGAGATA GTGCTGTAGA CAAGGGTGAT 
ATTGAATCCT TAAGGAAATT CTCATGGCAA GAATATATGA AGAAAAGGAA ACAGAAGAAA 
GTACTAGAGC TAAAGGATGA TATAGAAGAT GAACCATACC TTTTTGGAGA TGAAAAGCTC 
ACTGAAAGAG AAATTCGTGA ATTTAGATAC AAGAGAGAAA TCTATGAGCT CATAAAGAAA 
AGTACACAAG AAGAAGATAA TGTAGGAGAG TATAGAATGC CGGATGCATA TGATCAACAG 
GGAAGTGTTG ATCAGGAGAA GAGGTTTGCT GTTTCTGTAC AACGGTACAG GGACATGGGT 
TCCGCAGAGA AAATGAACCC CTTTGCAGAG CAAGAAGCTT GGGAGGACCA TCAGATTGAA 
AACGCAGCAT TAAAATTTGG TGCAAAGAAT AAGGAGGTCT CAGACAATTA CGAGTTTGTA 
TTTGAAGACC AAATAGACTT CATAAAGGCA TCTGTGTTGG CTGGTGATAA TTATGAAGAT 
GAAATGCATG CTAAACCATC TCAAGATTCT GCTGGGAAAT CGGCTTTTCA TATGCTTCAG 
GAGGATAGAA AAGCTCTGCC AATTTATACA TATCGTGATC AACTTCTTAA TGCTGTGAAA 
GATCATCAGG TTCTTATCAT TGTGGGGGAA ACGGGTTCTG GCAAGACTAC ACAGATACCC 
CAATATCTTC ACGAAGCTGG TTACACAAAG CTTGGAAAGG TTGGTTGTAC GCAGCCTCGA 
AGAGTTGCGG CTATGAGTGT GGCTGCTCGT GTTGCTCAAG AGATGGGTGG TAAACTTGGG 
CATGAGGTCG GCTATTCCAT TCGATTTGAG GATTGTACGT CTGAGAAAAC AATTCTGAAA 
TATATGACTG ATGGAATGCT TTTGCGTGAG TTGCTTGGTG AGCCAGATCT GGGCAGTTAC 
AGTGTTATCA TCGTGGATGA GGCGCATGAA AGAACTTTGC GCACCGACAT ACTATTTGGA 
TTAGTGAAGG ATATAGCACG GGCTCGGCCT GATCTGAAGT TATTGATATC CAGTGCAACC 
ATGGATGCAG AGAAGTTTTC TGATTTTTTT GACCAAGCCC CTATCTTCAG GTTCCCAGGA 
AGAAGGTATC CTGTTGACAT TTGCTTTACT ACTGCACCTG AAGCTGATTA CATGGACGCA 
GCAATAACTA CTGTGCTTAC GATACATGTG AAGGAGCCAC TTGGGGATGT TTTGGTCTTC 
CTCCCAGGTC AAGAGGAAAT TGAAGCCGTA GAAGAGAACT TGAAGCACAA GATAAGAGGT 
TTGGGTACAA AGATCCGCGA ACTCATTATA TGCCCAATTT ACGCTAACCT TCCAAGTGAG 
CTCCAAGCAA AAATATTTGA ACCAACTCCT GAAGGGGCAC GAAAAGTCGT GCTAGCAACT 
AATATTGCTG AAACATCATT GACCATCGAT GGTATAAAGT ATGTCGTTGA TCCAGGCTTT 
AGCAAGATGA AATCCTATAA CCCTAGAACA GGGATGGAAT CTTTGCTTGT AACGCCTATC 
TCTAAGGCTT CAGCGACTCA ACGAACTGGT CGAGCTGGAA GAACCAGTCC AGGGAAGTGT 
TACCGCCTGT ACACGGCGTT TAATTACTAC AATGACTTGG AGGACAATAC CGTACCTGAA 
ATACAAAGGA CTAATCTTGC TAGTGTGGTG CTTTCTTTGA AGAGTCTTGG TATTCATAAT 
CTGCTGAACT TTGACTTTAT GGACCCTCCG CCTTCTGAAG CTCTTATAAA GTCCTTAGAG 
CTGCTCTTTG CTCTGGGTGC TCTCAATCAG CTTGGCGAAT TGACTAAAGC TGGTAGACGG 
ATGGCAGAGT TTCCTCTTGA TCCAATGTTG TCGAAGATGA TTGTTGTTTC TGACAAATAC 
AAATGCTCCG ATGAGATTAT ATCAATTGCT GCTATGTTGT CCATCGGGCC TTCTATCTTT 
TACCGCCCCA AGGACAAGCA AGTTCACGCT GACAATGCAA TGAAGAATTT TCACGTTGGA 
AATGTCGGTG ATCACATTGC TTTCCTGAAG ATTTACAATT CGTGGAAGGA AACGAACTAC 
TCAACACAAT GGTGCTACGA GAACTATATT CAGGTTCGGA GTATGAAAAG AGCCAGAGAT 
ATCCGTGATC AGTTGGAGGG TCTTCTCGAG AGAGTTGAAA TAGACGTTAG CTCAAACGCG 
AATGAGTTGG ATTCAATAAG AAAATCCATT GTAGCTGGTT TCTTTCCACA CACTGCGAAG 
CTACAGAAGA ATGGATCATA TCGAACAGTG AAGCATCCCC AAACCGTACA CATACATCCA 
GCCTCTGGCT TATCTCAGGT ATTGCCGAGA TGGGTTGTAT ATCATCAACT AGTCCTTACC 
TCTAAGGAAT ACATGCGACA GGTAACAGAG TTAAAACCCG AGTGGTTGAT TGAGATAGCT 
CCACACTATT ATCAGCTCAA GGACGTTGAA GATGCTACAT CCAAGAAAAT GCCCAAAACC 
AGTGGCCGAG CTGTGGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001323587.1
CDS	20..2740
Translation	MQVVIVREDKRNVRRKVSEDEDDGTESEEERLRDQREREELEQHLRERDTARTRKLTEPKMSKKEQEEFVRRDSAVDKGDIESLRKFSWQEYMKKRKQKKVLELKDDIEDEPYLFGDEKLTEREIREFRYKREIYELIKKSTQEEDNVGEYRMPDAYDQQGSVDQEKRFAVSVQRYRDMGSAEKMNPFAEQEAWEDHQIENAALKFGAKNKEVSDNYEFVFEDQIDFIKASVLAGDNYEDEMHAKPSQDSAGKSAFHMLQEDRKALPIYTYRDQLLNAVKDHQVLIIVGETGSGKTTQIPQYLHEAGYTKLGKVGCTQPRRVAAMSVAARVAQEMGGKLGHEVGYSIRFEDCTSEKTILKYMTDGMLLRELLGEPDLGSYSVIIVDEAHERTLRTDILFGLVKDIARARPDLKLLISSATMDAEKFSDFFDQAPIFRFPGRRYPVDICFTTAPEADYMDAAITTVLTIHVKEPLGDVLVFLPGQEEIEAVEENLKHKIRGLGTKIRELIICPIYANLPSELQAKIFEPTPEGARKVVLATNIAETSLTIDGIKYVVDPGFSKMKSYNPRTGMESLLVTPISKASATQRTGRAGRTSPGKCYRLYTAFNYYNDLEDNTVPEIQRTNLASVVLSLKSLGIHNLLNFDFMDPPPSEALIKSLELLFALGALNQLGELTKAGRRMAEFPLDPMLSKMIVVSDKYKCSDEIISIAAMLSIGPSIFYRPKDKQVHADNAMKNFHVGNVGDHIAFLKIYNSWKETNYSTQWCYENYIQVRSMKRARDIRDQLEGLLERVEIDVSSNANELDSIRKSIVAGFFPHTAKLQKNGSYRTVKHPQTVHIHPASGLSQVLPRWVVYHQLVLTSKEYMRQVTELKPEWLIEIAPHYYQLKDVEDATSKKMPKTSGRAVV

Target ORF information:

RefSeq Version	NM_001336552.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana helicase domain-containing protein (MEE29), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001336552.1

ORF Insert Sequence:

ATGCAGGTAG TGATTGTTAG AGAGGACAAA AGGAATGTTA GGAGAAAGGT TTCCGAAGAC 
GAGGATGATG GCACTGAGTC TGAAGAGGAA AGATTACGTG ACCAAAGGGA GAGAGAAGAA 
CTAGAGCAGC ACCTAAGAGA ACGAGATACT GCACGGACAC GAAAGCTAAC CGAGCCAAAA 
ATGTCAAAGA AAGAGCAAGA AGAGTTTGTT CGAAGAGATA GTGCTGTAGA CAAGGGTGAT 
ATTGAATCCT TAAGGAAATT CTCATGGCAA GAATATATGA AGAAAAGGAA ACAGAAGAAA 
GTACTAGAGC TAAAGGATGA TATAGAAGAT GAACCATACC TTTTTGGAGA TGAAAAGCTC 
ACTGAAAGAG AAATTCGTGA ATTTAGATAC AAGAGAGAAA TCTATGAGCT CATAAAGAAA 
AGTACACAAG AAGAAGATAA TGTAGGAGAG TATAGAATGC CGGATGCATA TGATCAACAG 
GGAAGTGTTG ATCAGGAGAA GAGGTTTGCT GTTTCTGTAC AACGGTACAG GGACATGGGT 
TCCGCAGAGA AAATGAACCC CTTTGCAGAG CAAGAAGCTT GGGAGGACCA TCAGATTGAA 
AACGCAGCAT TAAAATTTGG TGCAAAGAAT AAGGAGGTCT CAGACAATTA CGAGTTTGTA 
TTTGAAGACC AAATAGACTT CATAAAGGCA TCTGTGTTGG CTGGTGATAA TTATGAAGAT 
GAAATGCATG CTAAACCATC TCAAGATTCT GCTGGGAAAT CGGCTTTTCA TATGCTTCAG 
GAGGATAGAA AAGCTCTGCC AATTTATACA TATCGTGATC AACTTCTTAA TGCTGTGAAA 
GATCATCAGG TTCTTATCAT TGTGGGGGAA ACGGGTTCTG GCAAGACTAC ACAGATACCC 
CAATATCTTC ACGAAGCTGG TTACACAAAG CTTGGAAAGG TTGGTTGTAC GCAGCCTCGA 
AGAGTTGCGG CTATGAGTGT GGCTGCTCGT GTTGCTCAAG AGATGGGTGG TAAACTTGGG 
CATGAGGTCG GCTATTCCAT TCGATTTGAG GATTGTACGT CTGAGAAAAC AATTCTGAAA 
TATATGACTG ATGGAATGCT TTTGCGTGAG TTGCTTGGTG AGCCAGATCT GGGCAGTTAC 
AGTGTTATCA TCGTGGATGA GGCGCATGAA AGAACTTTGC GCACCGACAT ACTATTTGGA 
TTAGTGAAGG ATATAGCACG GGCTCGGCCT GATCTGAAGT TATTGATATC CAGTGCAACC 
ATGGATGCAG AGAAGTTTTC TGATTTTTTT GACCAAGCCC CTATCTTCAG GTTCCCAGGA 
AGAAGGTATC CTGTTGACAT TTGCTTTACT ACTGCACCTG AAGCTGATTA CATGGACGCA 
GCAATAACTA CTGTGCTTAC GATACATGTG AAGGAGCCAC TTGGGGATGT TTTGGTCTTC 
CTCCCAGGTC AAGAGGAAAT TGAAGCCGTA GAAGAGAACT TGAAGCACAA GATAAGAGGT 
TTGGGTACAA AGATCCGCGA ACTCATTATA TGCCCAATTT ACGCTAACCT TCCAAGTGAG 
CTCCAAGCAA AAATATTTGA ACCAACTCCT GAAGGGGCAC GAAAAGTCGT GCTAGCAACT 
AATATTGCTG AAACATCATT GACCATCGAT GGTATAAAGT ATGTCGTTGA TCCAGGCTTT 
AGCAAGATGA AATCCTATAA CCCTAGAACA GGGATGGAAT CTTTGCTTGT AACGCCTATC 
TCTAAGGCTT CAGCGACTCA ACGAACTGGT CGAGCTGGAA GAACCAGTCC AGGGAAGTGT 
TACCGCCTGT ACACGGCGTT TAATTACTAC AATGACTTGG AGGACAATAC CGTACCTGAA 
ATACAAAGGA CTAATCTTGC TAGTGTGGTG CTTTCTTTGA AGAGTCTTGG TATTCATAAT 
CTGCTGAACT TTGACTTTAT GGACCCTCCG CCTTCTGAAG CTCTTATAAA GTCCTTAGAG 
CTGCTCTTTG CTCTGGGTGC TCTCAATCAG CTTGGCGAAT TGACTAAAGC TGGTAGACGG 
ATGGCAGAGT TTCCTCTTGA TCCAATGTTG TCGAAGATGA TTGTTGTTTC TGACAAATAC 
AAATGCTCCG ATGAGATTAT ATCAATTGCT GCTATGTTGT CCATCGGGCC TTCTATCTTT 
TACCGCCCCA AGGACAAGCA AGTTCACGCT GACAATGCAA TGAAGAATTT TCACGTTGGA 
AATGTCGGTG ATCACATTGC TTTCCTGAAG ATTTACAATT CGTGGAAGGA AACGAACTAC 
TCAACACAAT GGTGCTACGA GAACTATATT CAGGTTCGGA GTATGAAAAG AGCCAGAGAT 
ATCCGTGATC AGTTGGAGGG TCTTCTCGAG AGAGTTGAAA TAGACGTTAG CTCAAACGCG 
AATGAGTTGG ATTCAATAAG AAAATCCATT GTAGCTGGTT TCTTTCCACA CACTGCGAAG 
CTACAGAAGA ATGGATCATA TCGAACAGTG AAGCATCCCC AAACCGTACA CATACATCCA 
GCCTCTGGCT TATCTCAGGT ATTGCCGAGA TGGGTTGTAT ATCATCAACT AGTCCTTACC 
TCTAAGGAAT ACATGCGACA GGTAACAGAG TTAAAACCCG AGTGGTTGAT TGAGATAGCT 
CCACACTATT ATCAGCTCAA GGACGTTGAA GATGCTACAT CCAAGAAAAT GCCCAAAACC 
AGTGGCCGAG CTGTGGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEE29 ( NM_129086.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_181077.3 Arabidopsis thaliana helicase domain-containing protein (MEE29), mRNA.

Price & Availability↑

CloneID	OAb08823
Clone ID Related Accession (Same CDS sequence)	NM_129086.4
Accession Version	NM_129086.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	helicase domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). On Sep 12, 2016 this sequence version replaced NM_129086.3.

ATGGGGAGCA ATGATTTGAA CACCTGGGTT TCGGACAAGT TGATGGTTCT GCTAGGCTTT 
TCACAAACTG CTGTCGTGCA GTATCTAATT GCAATGGCTA AACAATCAAA ATCACCAGGT 
GAACTCGTCC GTGAATTGGT GGAATGCGGG TTTTCTTTGT CCGGTGATAC CCGCGCGTTC 
GCAGAAGAAA TTTATGCTAG AGCTCCCCGG AAAACCCCTG GAGTGAATCT CTACCAGCAA 
CAGGAAGCAG AGGCAGCGAT GCTGTTGAAG AAGCAGAAAA CTTTTTCCCT TTTAGAGGCT 
GATCATGATG AAGATGAAAA CAATGTGAAG AAACAATCTG CTTCGAAAAC CGGAAAGTCA 
GATAAAGGCC AAAAGAGGTT CAGGAAGAAA AGTGAGCAAT TGGAAGACGA CGATGATGAG 
GTAGTGATTG TTAGAGAGGA CAAAAGGAAT GTTAGGAGAA AGGTTTCCGA AGACGAGGAT 
GATGGCACTG AGTCTGAAGA GGAAAGATTA CGTGACCAAA GGGAGAGAGA AGAACTAGAG 
CAGCACCTAA GAGAACGAGA TACTGCACGG ACACGAAAGC TAACCGAGCC AAAAATGTCA 
AAGAAAGAGC AAGAAGAGTT TGTTCGAAGA GATAGTGCTG TAGACAAGGG TGATATTGAA 
TCCTTAAGGA AATTCTCATG GCAAGAATAT ATGAAGAAAA GGAAACAGAA GAAAGTACTA 
GAGCTAAAGG ATGATATAGA AGATGAACCA TACCTTTTTG GAGATGAAAA GCTCACTGAA 
AGAGAAATTC GTGAATTTAG ATACAAGAGA GAAATCTATG AGCTCATAAA GAAAAGTACA 
CAAGAAGAAG ATAATGTAGG AGAGTATAGA ATGCCGGATG CATATGATCA ACAGGGAAGT 
GTTGATCAGG AGAAGAGGTT TGCTGTTTCT GTACAACGGT ACAGGGACAT GGGTTCCGCA 
GAGAAAATGA ACCCCTTTGC AGAGCAAGAA GCTTGGGAGG ACCATCAGAT TGAAAACGCA 
GCATTAAAAT TTGGTGCAAA GAATAAGGAG GTCTCAGACA ATTACGAGTT TGTATTTGAA 
GACCAAATAG ACTTCATAAA GGCATCTGTG TTGGCTGGTG ATAATTATGA AGATGAAATG 
CATGCTAAAC CATCTCAAGA TTCTGCTGGG AAATCGGCTT TTCATATGCT TCAGGAGGAT 
AGAAAAGCTC TGCCAATTTA TACATATCGT GATCAACTTC TTAATGCTGT GAAAGATCAT 
CAGGTTCTTA TCATTGTGGG GGAAACGGGT TCTGGCAAGA CTACACAGAT ACCCCAATAT 
CTTCACGAAG CTGGTTACAC AAAGCTTGGA AAGGTTGGTT GTACGCAGCC TCGAAGAGTT 
GCGGCTATGA GTGTGGCTGC TCGTGTTGCT CAAGAGATGG GTGGTAAACT TGGGCATGAG 
GTCGGCTATT CCATTCGATT TGAGGATTGT ACGTCTGAGA AAACAATTCT GAAATATATG 
ACTGATGGAA TGCTTTTGCG TGAGTTGCTT GGTGAGCCAG ATCTGGGCAG TTACAGTGTT 
ATCATCGTGG ATGAGGCGCA TGAAAGAACT TTGCGCACCG ACATACTATT TGGATTAGTG 
AAGGATATAG CACGGGCTCG GCCTGATCTG AAGTTATTGA TATCCAGTGC AACCATGGAT 
GCAGAGAAGT TTTCTGATTT TTTTGACCAA GCCCCTATCT TCAGGTTCCC AGGAAGAAGG 
TATCCTGTTG ACATTTGCTT TACTACTGCA CCTGAAGCTG ATTACATGGA CGCAGCAATA 
ACTACTGTGC TTACGATACA TGTGAAGGAG CCACTTGGGG ATGTTTTGGT CTTCCTCCCA 
GGTCAAGAGG AAATTGAAGC CGTAGAAGAG AACTTGAAGC ACAAGATAAG AGGTTTGGGT 
ACAAAGATCC GCGAACTCAT TATATGCCCA ATTTACGCTA ACCTTCCAAG TGAGCTCCAA 
GCAAAAATAT TTGAACCAAC TCCTGAAGGG GCACGAAAAG TCGTGCTAGC AACTAATATT 
GCTGAAACAT CATTGACCAT CGATGGTATA AAGTATGTCG TTGATCCAGG CTTTAGCAAG 
ATGAAATCCT ATAACCCTAG AACAGGGATG GAATCTTTGC TTGTAACGCC TATCTCTAAG 
GCTTCAGCGA CTCAACGAAC TGGTCGAGCT GGAAGAACCA GTCCAGGGAA GTGTTACCGC 
CTGTACACGG CGTTTAATTA CTACAATGAC TTGGAGGACA ATACCGTACC TGAAATACAA 
AGGACTAATC TTGCTAGTGT GGTGCTTTCT TTGAAGAGTC TTGGTATTCA TAATCTGCTG 
AACTTTGACT TTATGGACCC TCCGCCTTCT GAAGCTCTTA TAAAGTCCTT AGAGCTGCTC 
TTTGCTCTGG GTGCTCTCAA TCAGCTTGGC GAATTGACTA AAGCTGGTAG ACGGATGGCA 
GAGTTTCCTC TTGATCCAAT GTTGTCGAAG ATGATTGTTG TTTCTGACAA ATACAAATGC 
TCCGATGAGA TTATATCAAT TGCTGCTATG TTGTCCATCG GGCCTTCTAT CTTTTACCGC 
CCCAAGGACA AGCAAGTTCA CGCTGACAAT GCAATGAAGA ATTTTCACGT TGGAAATGTC 
GGTGATCACA TTGCTTTCCT GAAGATTTAC AATTCGTGGA AGGAAACGAA CTACTCAACA 
CAATGGTGCT ACGAGAACTA TATTCAGGTT CGGAGTATGA AAAGAGCCAG AGATATCCGT 
GATCAGTTGG AGGGTCTTCT CGAGAGAGTT GAAATAGACG TTAGCTCAAA CGCGAATGAG 
TTGGATTCAA TAAGAAAATC CATTGTAGCT GGTTTCTTTC CACACACTGC GAAGCTACAG 
AAGAATGGAT CATATCGAAC AGTGAAGCAT CCCCAAACCG TACACATACA TCCAGCCTCT 
GGCTTATCTC AGGTATTGCC GAGATGGGTT GTATATCATC AACTAGTCCT TACCTCTAAG 
GAATACATGC GACAGGTAAC AGAGTTAAAA CCCGAGTGGT TGATTGAGAT AGCTCCACAC 
TATTATCAGC TCAAGGACGT TGAAGATGCT ACATCCAAGA AAATGCCCAA AACCAGTGGC 
CGAGCTGTGG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_181077.3
CDS	166..3300
Translation	MGSNDLNTWVSDKLMVLLGFSQTAVVQYLIAMAKQSKSPGELVRELVECGFSLSGDTRAFAEEIYARAPRKTPGVNLYQQQEAEAAMLLKKQKTFSLLEADHDEDENNVKKQSASKTGKSDKGQKRFRKKSEQLEDDDDEVVIVREDKRNVRRKVSEDEDDGTESEEERLRDQREREELEQHLRERDTARTRKLTEPKMSKKEQEEFVRRDSAVDKGDIESLRKFSWQEYMKKRKQKKVLELKDDIEDEPYLFGDEKLTEREIREFRYKREIYELIKKSTQEEDNVGEYRMPDAYDQQGSVDQEKRFAVSVQRYRDMGSAEKMNPFAEQEAWEDHQIENAALKFGAKNKEVSDNYEFVFEDQIDFIKASVLAGDNYEDEMHAKPSQDSAGKSAFHMLQEDRKALPIYTYRDQLLNAVKDHQVLIIVGETGSGKTTQIPQYLHEAGYTKLGKVGCTQPRRVAAMSVAARVAQEMGGKLGHEVGYSIRFEDCTSEKTILKYMTDGMLLRELLGEPDLGSYSVIIVDEAHERTLRTDILFGLVKDIARARPDLKLLISSATMDAEKFSDFFDQAPIFRFPGRRYPVDICFTTAPEADYMDAAITTVLTIHVKEPLGDVLVFLPGQEEIEAVEENLKHKIRGLGTKIRELIICPIYANLPSELQAKIFEPTPEGARKVVLATNIAETSLTIDGIKYVVDPGFSKMKSYNPRTGMESLLVTPISKASATQRTGRAGRTSPGKCYRLYTAFNYYNDLEDNTVPEIQRTNLASVVLSLKSLGIHNLLNFDFMDPPPSEALIKSLELLFALGALNQLGELTKAGRRMAEFPLDPMLSKMIVVSDKYKCSDEIISIAAMLSIGPSIFYRPKDKQVHADNAMKNFHVGNVGDHIAFLKIYNSWKETNYSTQWCYENYIQVRSMKRARDIRDQLEGLLERVEIDVSSNANELDSIRKSIVAGFFPHTAKLQKNGSYRTVKHPQTVHIHPASGLSQVLPRWVVYHQLVLTSKEYMRQVTELKPEWLIEIAPHYYQLKDVEDATSKKMPKTSGRAVV

Target ORF information:

RefSeq Version	NM_129086.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana helicase domain-containing protein (MEE29), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_129086.4

ORF Insert Sequence:

ATGGGGAGCA ATGATTTGAA CACCTGGGTT TCGGACAAGT TGATGGTTCT GCTAGGCTTT 
TCACAAACTG CTGTCGTGCA GTATCTAATT GCAATGGCTA AACAATCAAA ATCACCAGGT 
GAACTCGTCC GTGAATTGGT GGAATGCGGG TTTTCTTTGT CCGGTGATAC CCGCGCGTTC 
GCAGAAGAAA TTTATGCTAG AGCTCCCCGG AAAACCCCTG GAGTGAATCT CTACCAGCAA 
CAGGAAGCAG AGGCAGCGAT GCTGTTGAAG AAGCAGAAAA CTTTTTCCCT TTTAGAGGCT 
GATCATGATG AAGATGAAAA CAATGTGAAG AAACAATCTG CTTCGAAAAC CGGAAAGTCA 
GATAAAGGCC AAAAGAGGTT CAGGAAGAAA AGTGAGCAAT TGGAAGACGA CGATGATGAG 
GTAGTGATTG TTAGAGAGGA CAAAAGGAAT GTTAGGAGAA AGGTTTCCGA AGACGAGGAT 
GATGGCACTG AGTCTGAAGA GGAAAGATTA CGTGACCAAA GGGAGAGAGA AGAACTAGAG 
CAGCACCTAA GAGAACGAGA TACTGCACGG ACACGAAAGC TAACCGAGCC AAAAATGTCA 
AAGAAAGAGC AAGAAGAGTT TGTTCGAAGA GATAGTGCTG TAGACAAGGG TGATATTGAA 
TCCTTAAGGA AATTCTCATG GCAAGAATAT ATGAAGAAAA GGAAACAGAA GAAAGTACTA 
GAGCTAAAGG ATGATATAGA AGATGAACCA TACCTTTTTG GAGATGAAAA GCTCACTGAA 
AGAGAAATTC GTGAATTTAG ATACAAGAGA GAAATCTATG AGCTCATAAA GAAAAGTACA 
CAAGAAGAAG ATAATGTAGG AGAGTATAGA ATGCCGGATG CATATGATCA ACAGGGAAGT 
GTTGATCAGG AGAAGAGGTT TGCTGTTTCT GTACAACGGT ACAGGGACAT GGGTTCCGCA 
GAGAAAATGA ACCCCTTTGC AGAGCAAGAA GCTTGGGAGG ACCATCAGAT TGAAAACGCA 
GCATTAAAAT TTGGTGCAAA GAATAAGGAG GTCTCAGACA ATTACGAGTT TGTATTTGAA 
GACCAAATAG ACTTCATAAA GGCATCTGTG TTGGCTGGTG ATAATTATGA AGATGAAATG 
CATGCTAAAC CATCTCAAGA TTCTGCTGGG AAATCGGCTT TTCATATGCT TCAGGAGGAT 
AGAAAAGCTC TGCCAATTTA TACATATCGT GATCAACTTC TTAATGCTGT GAAAGATCAT 
CAGGTTCTTA TCATTGTGGG GGAAACGGGT TCTGGCAAGA CTACACAGAT ACCCCAATAT 
CTTCACGAAG CTGGTTACAC AAAGCTTGGA AAGGTTGGTT GTACGCAGCC TCGAAGAGTT 
GCGGCTATGA GTGTGGCTGC TCGTGTTGCT CAAGAGATGG GTGGTAAACT TGGGCATGAG 
GTCGGCTATT CCATTCGATT TGAGGATTGT ACGTCTGAGA AAACAATTCT GAAATATATG 
ACTGATGGAA TGCTTTTGCG TGAGTTGCTT GGTGAGCCAG ATCTGGGCAG TTACAGTGTT 
ATCATCGTGG ATGAGGCGCA TGAAAGAACT TTGCGCACCG ACATACTATT TGGATTAGTG 
AAGGATATAG CACGGGCTCG GCCTGATCTG AAGTTATTGA TATCCAGTGC AACCATGGAT 
GCAGAGAAGT TTTCTGATTT TTTTGACCAA GCCCCTATCT TCAGGTTCCC AGGAAGAAGG 
TATCCTGTTG ACATTTGCTT TACTACTGCA CCTGAAGCTG ATTACATGGA CGCAGCAATA 
ACTACTGTGC TTACGATACA TGTGAAGGAG CCACTTGGGG ATGTTTTGGT CTTCCTCCCA 
GGTCAAGAGG AAATTGAAGC CGTAGAAGAG AACTTGAAGC ACAAGATAAG AGGTTTGGGT 
ACAAAGATCC GCGAACTCAT TATATGCCCA ATTTACGCTA ACCTTCCAAG TGAGCTCCAA 
GCAAAAATAT TTGAACCAAC TCCTGAAGGG GCACGAAAAG TCGTGCTAGC AACTAATATT 
GCTGAAACAT CATTGACCAT CGATGGTATA AAGTATGTCG TTGATCCAGG CTTTAGCAAG 
ATGAAATCCT ATAACCCTAG AACAGGGATG GAATCTTTGC TTGTAACGCC TATCTCTAAG 
GCTTCAGCGA CTCAACGAAC TGGTCGAGCT GGAAGAACCA GTCCAGGGAA GTGTTACCGC 
CTGTACACGG CGTTTAATTA CTACAATGAC TTGGAGGACA ATACCGTACC TGAAATACAA 
AGGACTAATC TTGCTAGTGT GGTGCTTTCT TTGAAGAGTC TTGGTATTCA TAATCTGCTG 
AACTTTGACT TTATGGACCC TCCGCCTTCT GAAGCTCTTA TAAAGTCCTT AGAGCTGCTC 
TTTGCTCTGG GTGCTCTCAA TCAGCTTGGC GAATTGACTA AAGCTGGTAG ACGGATGGCA 
GAGTTTCCTC TTGATCCAAT GTTGTCGAAG ATGATTGTTG TTTCTGACAA ATACAAATGC 
TCCGATGAGA TTATATCAAT TGCTGCTATG TTGTCCATCG GGCCTTCTAT CTTTTACCGC 
CCCAAGGACA AGCAAGTTCA CGCTGACAAT GCAATGAAGA ATTTTCACGT TGGAAATGTC 
GGTGATCACA TTGCTTTCCT GAAGATTTAC AATTCGTGGA AGGAAACGAA CTACTCAACA 
CAATGGTGCT ACGAGAACTA TATTCAGGTT CGGAGTATGA AAAGAGCCAG AGATATCCGT 
GATCAGTTGG AGGGTCTTCT CGAGAGAGTT GAAATAGACG TTAGCTCAAA CGCGAATGAG 
TTGGATTCAA TAAGAAAATC CATTGTAGCT GGTTTCTTTC CACACACTGC GAAGCTACAG 
AAGAATGGAT CATATCGAAC AGTGAAGCAT CCCCAAACCG TACACATACA TCCAGCCTCT 
GGCTTATCTC AGGTATTGCC GAGATGGGTT GTATATCATC AACTAGTCCT TACCTCTAAG 
GAATACATGC GACAGGTAAC AGAGTTAAAA CCCGAGTGGT TGATTGAGAT AGCTCCACAC 
TATTATCAGC TCAAGGACGT TGAAGATGCT ACATCCAAGA AAATGCCCAA AACCAGTGGC 
CGAGCTGTGG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Nature402(6763)761-8(1999 Dec)
Lin X,Kaul S,Rounsley S,Shea TP,Benito MI,Town CD,Fujii CY,Mason T,Bowman CL,Barnstead M,Feldblyum TV,Buell CR,Ketchum KA,Lee J,Ronning CM,Koo HL,Moffat KS,Cronin LA,Shen M,Pai G,Van Aken S,Umayam L,Tallon LJ,Gill JE,Adams MD,Carrera AJ,Creasy TH,Goodman HM,Somerville CR,Copenhaver GP,Preuss D,Nierman WC,White O,Eisen JA,Salzberg SL,Fraser CM,Venter JC

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MEE29 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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