HBT cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	HBT
Entrez Gene ID	816519
Full Name	CDC27 family protein
Synonyms	CDC27b,HOBBIT,T2G17.20,T2G17_20
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_179663.3	NP_849994.1	CDC27 family protein
NM_001335657.1	NP_001323708.1	CDC27 family protein

Pathways from BioSystems

Homologies

	CDC27	XP_001115976.2
Canis lupus familiaris (dog)	CDC27	XP_548047.2
Rattus norvegicus (Norway rat)	Cdc27	NP_001019964.1
Homo sapiens (human)	CDC27	NP_001107563.1
Bos taurus (cattle)	CDC27	NP_001098898.1
Gallus gallus (chicken)	CDC27	NP_001006331.1
Danio rerio (zebrafish)	cdc27	NP_958857.1
Mus musculus (house mouse)	Cdc27	NP_663411.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007690	XP_308181.4
Oryza sativa (rice)	Os06g0622500	NP_001058099.2
Xenopus tropicalis (tropical clawed frog)	cdc27	NP_001072470.1
Pan troglodytes (chimpanzee)	CDC27	XP_511624.2
Drosophila melanogaster (fruit fly)	Cdc27	NP_001261503.1
Arabidopsis thaliana (thale cress)	HBT	NP_849994.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Lin X, Kaul S, Rounsley S, Shea TP, Benito MI, Town CD, Fujii CY, Mason T, Bowman CL, Barnstead M, Feldblyum TV, Buell CR, Ketchum KA, Lee J, Ronning CM, Koo HL, Moffat KS, Cronin LA, Shen M, Pai G, Van Aken S, Umayam L, Tallon LJ, Gill JE, Adams MD, Carrera AJ, Creasy TH, Goodman HM, Somerville CR, Copenhaver GP, Preuss D, Nierman WC, White O, Eisen JA, Salzberg SL, Fraser CM, Venter JC
Nature402(6763)761-8(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Small clones reveal non-cell-autonomous rescue of hbt mutant cells, indicating a previously unrecognized compensation mechanism for reduced activity of an anaphase-promoting complex/cyclosome component critical for cell cycle progression.
Title: Non-cell-autonomous rescue of anaphase-promoting complex function revealed by mosaic analysis of HOBBIT, an Arabidopsis CDC27 homolog.

The following HBT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HBT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb07440	NM_179663.3 Latest version!	Arabidopsis thaliana CDC27 family protein (HBT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb35816	NM_001335657.1 Latest version!	Arabidopsis thaliana CDC27 family protein (HBT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HBT ( NM_179663.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_849994.1 Arabidopsis thaliana CDC27 family protein (HBT), mRNA.

Price & Availability↑

CloneID	OAb07440
Clone ID Related Accession (Same CDS sequence)	NM_179663.3
Accession Version	NM_179663.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	CDC27 family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). On Sep 12, 2016 this sequence version replaced NM_179663.2.

ATGGAAGCTA TGCTTGTGGA CTGTGTAAAC AACAGTCTTC GTCATTTTGT CTACAAAAAT 
GCTATTTTCA TGTGCGAGCG TCTCTGCGCT GAGTTTCCTT CTGAGGTTAA TTTGCAGCTA 
TTAGCCACCA GCTACCTGCA GAATAATCAA GCTTACAGTG CATATCATCT GCTAAAGGGA 
ACACAAATGG CTCAGTCCCG ATACTTGTTC GCATTATCAT GCTTCCAGAT GGACCTTCTC 
AATGAAGCTG AATCTGCACT CTGCCCTGTT AATGAACCTG GTGCGGAGAT CCCAAATGGT 
GCAGCAGGCC ATTACCTTCT TGGACTTATT TACAAGTATA CTGATAGAAG GAAGAATGCT 
GCTCAACAAT TTAAACAGTC CTTGACAATA GACCCTCTAC TTTGGGCTGC ATATGAGGAA 
TTATGTATAT TAGGTGCTGC TGAGGAAGCA ACTGCAGTTT TTGGTGAAAC AGCTGCTCTC 
TCCATTCAAA AGCAGTATAT GCAACAACTG TCAACTTCCC TCGGCTTAAA CACTTACAAC 
GAGGAACGTA ATTCAACTTC TACTAAAAAC ACGAGTTCTG AAGATTATAG TCCAAGGCAG 
TCTAAACACA CACAAAGCCA TGGCCTTAAA GATATCTCCG GAAATTTCCA TTCTCATGGA 
GTTAATGGAG GTGTTTCGAA CATGTCATTC TATAATACGC CTTCGCCAGT GGCTGCACAG 
CTATCCGGTA TAGCTCCACC ACCACTTTTC CGGAATTTTC AGCCAGCTGT TGCAAACCCA 
AACTCCCTTA TTACTGACAG TTCTCCAAAG TCCACTGTTA ACTCTACTCT TCAAGCACCT 
AGAAGAAAGT TTGTAGATGA AGGAAAGTTA CGTAAGATTT CTGGCAGACT ATTTTCTGAT 
TCTGGTCCAC GACGGAGTTC AAGACTGTCT GCTGATTCAG GGGCAAACAT TAATTCAAGT 
GTTGCAACAG TAAGCGGAAA TGTGAACAAC GCTTCCAAGT ATTTGGGAGG TTCTAAATTG 
AGTTCTTTGG CACTTCGTTC TGTAACACTT CGGAAGGGAC ACTCCTGGGC AAATGAAAAC 
ATGGATGAAG GGGTCCGTGG GGAACCTTTT GATGATTCAA GGCCTAATAC TGCCTCAACG 
ACTGGTTCTA TGGCTTCCAA TGATCAAGAA GACGAAACAA TGTCGATTGG TGGCATAGCA 
ATGAGTTCTC AAACAATCAC AATTGGTGTT TCGGAAATTT TAAACCTCCT TAGGACACTC 
GGAGAAGGGT GTAGACTTTC ATACATGTAC AGGTGTCAGG AGGCACTGGA TACGTATATG 
AAACTTCCAC ATAAGCATTA TAATACAGGA TGGGTTCTTT CCCAGGTCGG GAAAGCATAC 
TTTGAACTAA TTGACTATTT AGAGGCTGAA AAGGCATTCC GTCTTGCCCG TCTGGCTTCT 
CCTTATTGCT TAGAAGGAAT GGATATATAC TCTACGGTCC TCTATCATTT GAAGGAAGAC 
ATGAAGCTGA GTTACTTGGC TCAGGAACTA ATATCAACCG ATCGCTTAGC TCCTCAATCT 
TGGTGTGCTA TGGGAAATTG CTATAGCTTG CAAAAGGACC ATGAGACCGC ACTGAAGAAT 
TTCCTACGAG CTGTTCAACT GAATCCAAGA TTTGCATATG CACATACCTT ATGTGGCCAC 
GAATACACAA CTCTTGAGGA TTTTGAGAAC GGAATGAAAA GTTACCAAAA CGCACTTCGT 
GTAGATACAA GACACTACAA CGCATGGTAC GGGCTTGGAA TGATATATCT ACGCCAAGAG 
AAGTTAGAGT TCTCAGAGCA TCACTTCAGA ATGGCTTTCC TAATAAACCC GAGTTCCTCT 
GTTATAATGT CTTATTTAGG GACATCTTTG CATGCCTTGA AGAGAAGTGA GGAAGCACTA 
GAGATAATGG AGCAAGCCAT AGTAGCAGAT AGAAAAAACC CTCTTCCAAT GTACCAGAAA 
GCTAACATAC TTGTCTGCTT AGAAAGATTA GATGAAGCTC TAGAAGTTCT TGAGGAGCTC 
AAAGAGTATG CGCCTTCAGA GAGCAGCGTT TACGCTTTAA TGGGCAGGAT CTATAAGCGG 
CGAAACATGC ACGATAAAGC CATGCTTCAT TTCGGTCTAG CTTTAGATAT GAAACCGCCT 
GCAACTGACG TTGCTGCAAT AAAGGCTGCA ATGGAGAAAT TGCATGTTCC AGATGAGATC 
GATGAGAGCC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_849994.1
CDS	401..2635
Translation	MEAMLVDCVNNSLRHFVYKNAIFMCERLCAEFPSEVNLQLLATSYLQNNQAYSAYHLLKGTQMAQSRYLFALSCFQMDLLNEAESALCPVNEPGAEIPNGAAGHYLLGLIYKYTDRRKNAAQQFKQSLTIDPLLWAAYEELCILGAAEEATAVFGETAALSIQKQYMQQLSTSLGLNTYNEERNSTSTKNTSSEDYSPRQSKHTQSHGLKDISGNFHSHGVNGGVSNMSFYNTPSPVAAQLSGIAPPPLFRNFQPAVANPNSLITDSSPKSTVNSTLQAPRRKFVDEGKLRKISGRLFSDSGPRRSSRLSADSGANINSSVATVSGNVNNASKYLGGSKLSSLALRSVTLRKGHSWANENMDEGVRGEPFDDSRPNTASTTGSMASNDQEDETMSIGGIAMSSQTITIGVSEILNLLRTLGEGCRLSYMYRCQEALDTYMKLPHKHYNTGWVLSQVGKAYFELIDYLEAEKAFRLARLASPYCLEGMDIYSTVLYHLKEDMKLSYLAQELISTDRLAPQSWCAMGNCYSLQKDHETALKNFLRAVQLNPRFAYAHTLCGHEYTTLEDFENGMKSYQNALRVDTRHYNAWYGLGMIYLRQEKLEFSEHHFRMAFLINPSSSVIMSYLGTSLHALKRSEEALEIMEQAIVADRKNPLPMYQKANILVCLERLDEALEVLEELKEYAPSESSVYALMGRIYKRRNMHDKAMLHFGLALDMKPPATDVAAIKAAMEKLHVPDEIDESP

Target ORF information:

RefSeq Version	NM_179663.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana CDC27 family protein (HBT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_179663.3

ORF Insert Sequence:

ATGGAAGCTA TGCTTGTGGA CTGTGTAAAC AACAGTCTTC GTCATTTTGT CTACAAAAAT 
GCTATTTTCA TGTGCGAGCG TCTCTGCGCT GAGTTTCCTT CTGAGGTTAA TTTGCAGCTA 
TTAGCCACCA GCTACCTGCA GAATAATCAA GCTTACAGTG CATATCATCT GCTAAAGGGA 
ACACAAATGG CTCAGTCCCG ATACTTGTTC GCATTATCAT GCTTCCAGAT GGACCTTCTC 
AATGAAGCTG AATCTGCACT CTGCCCTGTT AATGAACCTG GTGCGGAGAT CCCAAATGGT 
GCAGCAGGCC ATTACCTTCT TGGACTTATT TACAAGTATA CTGATAGAAG GAAGAATGCT 
GCTCAACAAT TTAAACAGTC CTTGACAATA GACCCTCTAC TTTGGGCTGC ATATGAGGAA 
TTATGTATAT TAGGTGCTGC TGAGGAAGCA ACTGCAGTTT TTGGTGAAAC AGCTGCTCTC 
TCCATTCAAA AGCAGTATAT GCAACAACTG TCAACTTCCC TCGGCTTAAA CACTTACAAC 
GAGGAACGTA ATTCAACTTC TACTAAAAAC ACGAGTTCTG AAGATTATAG TCCAAGGCAG 
TCTAAACACA CACAAAGCCA TGGCCTTAAA GATATCTCCG GAAATTTCCA TTCTCATGGA 
GTTAATGGAG GTGTTTCGAA CATGTCATTC TATAATACGC CTTCGCCAGT GGCTGCACAG 
CTATCCGGTA TAGCTCCACC ACCACTTTTC CGGAATTTTC AGCCAGCTGT TGCAAACCCA 
AACTCCCTTA TTACTGACAG TTCTCCAAAG TCCACTGTTA ACTCTACTCT TCAAGCACCT 
AGAAGAAAGT TTGTAGATGA AGGAAAGTTA CGTAAGATTT CTGGCAGACT ATTTTCTGAT 
TCTGGTCCAC GACGGAGTTC AAGACTGTCT GCTGATTCAG GGGCAAACAT TAATTCAAGT 
GTTGCAACAG TAAGCGGAAA TGTGAACAAC GCTTCCAAGT ATTTGGGAGG TTCTAAATTG 
AGTTCTTTGG CACTTCGTTC TGTAACACTT CGGAAGGGAC ACTCCTGGGC AAATGAAAAC 
ATGGATGAAG GGGTCCGTGG GGAACCTTTT GATGATTCAA GGCCTAATAC TGCCTCAACG 
ACTGGTTCTA TGGCTTCCAA TGATCAAGAA GACGAAACAA TGTCGATTGG TGGCATAGCA 
ATGAGTTCTC AAACAATCAC AATTGGTGTT TCGGAAATTT TAAACCTCCT TAGGACACTC 
GGAGAAGGGT GTAGACTTTC ATACATGTAC AGGTGTCAGG AGGCACTGGA TACGTATATG 
AAACTTCCAC ATAAGCATTA TAATACAGGA TGGGTTCTTT CCCAGGTCGG GAAAGCATAC 
TTTGAACTAA TTGACTATTT AGAGGCTGAA AAGGCATTCC GTCTTGCCCG TCTGGCTTCT 
CCTTATTGCT TAGAAGGAAT GGATATATAC TCTACGGTCC TCTATCATTT GAAGGAAGAC 
ATGAAGCTGA GTTACTTGGC TCAGGAACTA ATATCAACCG ATCGCTTAGC TCCTCAATCT 
TGGTGTGCTA TGGGAAATTG CTATAGCTTG CAAAAGGACC ATGAGACCGC ACTGAAGAAT 
TTCCTACGAG CTGTTCAACT GAATCCAAGA TTTGCATATG CACATACCTT ATGTGGCCAC 
GAATACACAA CTCTTGAGGA TTTTGAGAAC GGAATGAAAA GTTACCAAAA CGCACTTCGT 
GTAGATACAA GACACTACAA CGCATGGTAC GGGCTTGGAA TGATATATCT ACGCCAAGAG 
AAGTTAGAGT TCTCAGAGCA TCACTTCAGA ATGGCTTTCC TAATAAACCC GAGTTCCTCT 
GTTATAATGT CTTATTTAGG GACATCTTTG CATGCCTTGA AGAGAAGTGA GGAAGCACTA 
GAGATAATGG AGCAAGCCAT AGTAGCAGAT AGAAAAAACC CTCTTCCAAT GTACCAGAAA 
GCTAACATAC TTGTCTGCTT AGAAAGATTA GATGAAGCTC TAGAAGTTCT TGAGGAGCTC 
AAAGAGTATG CGCCTTCAGA GAGCAGCGTT TACGCTTTAA TGGGCAGGAT CTATAAGCGG 
CGAAACATGC ACGATAAAGC CATGCTTCAT TTCGGTCTAG CTTTAGATAT GAAACCGCCT 
GCAACTGACG TTGCTGCAAT AAAGGCTGCA ATGGAGAAAT TGCATGTTCC AGATGAGATC 
GATGAGAGCC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HBT ( NM_001335657.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001323708.1 Arabidopsis thaliana CDC27 family protein (HBT), mRNA.

Price & Availability↑

CloneID	OAb35816
Clone ID Related Accession (Same CDS sequence)	NM_001335657.1
Accession Version	NM_001335657.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	CDC27 family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071).

ATGGAAGCTA TGCTTGTGGA CTGTGTAAAC AACAGTCTTC GTCATTTTGT CTACAAAAAT 
GCTATTTTCA TGTGCGAGCG TCTCTGCGCT GAGTTTCCTT CTGAGGTTAA TTTGCAGCTA 
TTAGCCACCA GCTACCTGCA GAATAATCAA GCTTACAGTG CATATCATCT GCTAAAGGGA 
ACACAAATGG CTCAGTCCCG ATACTTGTTC GCATTATCAT GCTTCCAGAT GGACCTTCTC 
AATGAAGCTG AATCTGCACT CTGCCCTGTT AATGAACCTG GTGCGGAGAT CCCAAATGGT 
GCAGCAGGCC ATTACCTTCT TGGACTTATT TACAAGTATA CTGATAGAAG GAAGAATGCT 
GCTCAACAAT TTAAACAGTC CTTGACAATA GACCCTCTAC TTTGGGCTGC ATATGAGGAA 
TTATGTATAT TAGGTGCTGC TGAGGAAGCA ACTGCAGTTT TTGGTGAAAC AGCTGCTCTC 
TCCATTCAAA AGCAGTATAT GCAACAACTG TCAACTTCCC TCGGCTTAAA CACTTACAAC 
GAGGAACGTA ATTCAACTTC TACTAAAAAC ACGAGTTCTG AAGATTATAG TCCAAGGCAG 
TCTAAACACA CACAAAGCCA TGGCCTTAAA GATATCTCCG GAAATTTCCA TTCTCATGGA 
GTTAATGGAG GTGTTTCGAA CATGTCATTC TATAATACGC CTTCGCCAGT GGCTGCACAG 
CTATCCGGTA TAGCTCCACC ACCACTTTTC CGGAATTTTC AGCCAGCTGT TGCAAACCCA 
AACTCCCTTA TTACTGACAG TTCTCCAAAG TCCACTGTTA ACTCTACTCT TCAAGCACCT 
AGAAGAAAGT TTGTAGATGA AGGAAAGTTA CGTAAGATTT CTGGCAGACT ATTTTCTGAT 
TCTGGTCCAC GACGGAGTTC AAGACTGTCT GCTGATTCAG GGGCAAACAT TAATTCAAGT 
GTTGCAACAG TAAGCGGAAA TGTGAACAAC GCTTCCAAGT ATTTGGGAGG TTCTAAATTG 
AGTTCTTTGG CACTTCGTTC TGTAACACTT CGGAAGGGAC ACTCCTGGGC AAATGAAAAC 
ATGGATGAAG GGGTCCGTGG GGAACCTTTT GATGATTCAA GGCCTAATAC TGCCTCAACG 
ACTGGTTCTA TGGCTTCCAA TGATCAAGAA GACGAAACAA TGTCGATTGG TGGCATAGCA 
ATGAGTTCTC AAACAATCAC AATTGGTGTT TCGGAAATTT TAAACCTCCT TAGGACACTC 
GGAGAAGGGT GTAGACTTTC ATACATGTAC AGGTGTCAGG AGGCACTGGA TACGTATATG 
AAACTTCCAC ATAAGCATTA TAATACAGGA TGGGTTCTTT CCCAGGTCGG GAAAGCATAC 
TTTGAACTAA TTGACTATTT AGAGGCTGAA AAGGCATTCC GTCTTGCCCG TCTGGCTTCT 
CCTTATTGCT TAGAAGGAAT GGATATATAC TCTACGGTCC TCTATCATTT GAAGGAAGAC 
ATGAAGCTGA GTTACTTGGC TCAGGAACTA ATATCAACCG ATCGCTTAGC TCCTCAATCT 
TGGTGTGCTA TGGGAAATTG CTATAGCTTG CAAAAGGACC ATGAGACCGC ACTGAAGAAT 
TTCCTACGAG CTGTTCAACT GAATCCAAGA TTTGCATATG CACATACCTT ATGTGGCCAC 
GAATACACAA CTCTTGAGGA TTTTGAGAAC GGAATGAAAA GTTACCAAAA CGCACTTCGT 
GTAGATACAA GACACTACAA CGCATGGTAC GGGCTTGGAA TGATATATCT ACGCCAAGAG 
AAGTTAGAGT TCTCAGAGCA TCACTTCAGA ATGGCTTTCC TAATAAACCC GAGTTCCTCT 
GTTATAATGT CTTATTTAGG GACATCTTTG CATGCCTTGA AGGTTATCTT ATTACTTTCA 
TCTTATCAGG TCTACAAGAA AAACAATCTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001323708.1
CDS	401..2353
Translation	MEAMLVDCVNNSLRHFVYKNAIFMCERLCAEFPSEVNLQLLATSYLQNNQAYSAYHLLKGTQMAQSRYLFALSCFQMDLLNEAESALCPVNEPGAEIPNGAAGHYLLGLIYKYTDRRKNAAQQFKQSLTIDPLLWAAYEELCILGAAEEATAVFGETAALSIQKQYMQQLSTSLGLNTYNEERNSTSTKNTSSEDYSPRQSKHTQSHGLKDISGNFHSHGVNGGVSNMSFYNTPSPVAAQLSGIAPPPLFRNFQPAVANPNSLITDSSPKSTVNSTLQAPRRKFVDEGKLRKISGRLFSDSGPRRSSRLSADSGANINSSVATVSGNVNNASKYLGGSKLSSLALRSVTLRKGHSWANENMDEGVRGEPFDDSRPNTASTTGSMASNDQEDETMSIGGIAMSSQTITIGVSEILNLLRTLGEGCRLSYMYRCQEALDTYMKLPHKHYNTGWVLSQVGKAYFELIDYLEAEKAFRLARLASPYCLEGMDIYSTVLYHLKEDMKLSYLAQELISTDRLAPQSWCAMGNCYSLQKDHETALKNFLRAVQLNPRFAYAHTLCGHEYTTLEDFENGMKSYQNALRVDTRHYNAWYGLGMIYLRQEKLEFSEHHFRMAFLINPSSSVIMSYLGTSLHALKVILLLSSYQVYKKNNL

Target ORF information:

RefSeq Version	NM_001335657.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana CDC27 family protein (HBT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001335657.1

ORF Insert Sequence:

ATGGAAGCTA TGCTTGTGGA CTGTGTAAAC AACAGTCTTC GTCATTTTGT CTACAAAAAT 
GCTATTTTCA TGTGCGAGCG TCTCTGCGCT GAGTTTCCTT CTGAGGTTAA TTTGCAGCTA 
TTAGCCACCA GCTACCTGCA GAATAATCAA GCTTACAGTG CATATCATCT GCTAAAGGGA 
ACACAAATGG CTCAGTCCCG ATACTTGTTC GCATTATCAT GCTTCCAGAT GGACCTTCTC 
AATGAAGCTG AATCTGCACT CTGCCCTGTT AATGAACCTG GTGCGGAGAT CCCAAATGGT 
GCAGCAGGCC ATTACCTTCT TGGACTTATT TACAAGTATA CTGATAGAAG GAAGAATGCT 
GCTCAACAAT TTAAACAGTC CTTGACAATA GACCCTCTAC TTTGGGCTGC ATATGAGGAA 
TTATGTATAT TAGGTGCTGC TGAGGAAGCA ACTGCAGTTT TTGGTGAAAC AGCTGCTCTC 
TCCATTCAAA AGCAGTATAT GCAACAACTG TCAACTTCCC TCGGCTTAAA CACTTACAAC 
GAGGAACGTA ATTCAACTTC TACTAAAAAC ACGAGTTCTG AAGATTATAG TCCAAGGCAG 
TCTAAACACA CACAAAGCCA TGGCCTTAAA GATATCTCCG GAAATTTCCA TTCTCATGGA 
GTTAATGGAG GTGTTTCGAA CATGTCATTC TATAATACGC CTTCGCCAGT GGCTGCACAG 
CTATCCGGTA TAGCTCCACC ACCACTTTTC CGGAATTTTC AGCCAGCTGT TGCAAACCCA 
AACTCCCTTA TTACTGACAG TTCTCCAAAG TCCACTGTTA ACTCTACTCT TCAAGCACCT 
AGAAGAAAGT TTGTAGATGA AGGAAAGTTA CGTAAGATTT CTGGCAGACT ATTTTCTGAT 
TCTGGTCCAC GACGGAGTTC AAGACTGTCT GCTGATTCAG GGGCAAACAT TAATTCAAGT 
GTTGCAACAG TAAGCGGAAA TGTGAACAAC GCTTCCAAGT ATTTGGGAGG TTCTAAATTG 
AGTTCTTTGG CACTTCGTTC TGTAACACTT CGGAAGGGAC ACTCCTGGGC AAATGAAAAC 
ATGGATGAAG GGGTCCGTGG GGAACCTTTT GATGATTCAA GGCCTAATAC TGCCTCAACG 
ACTGGTTCTA TGGCTTCCAA TGATCAAGAA GACGAAACAA TGTCGATTGG TGGCATAGCA 
ATGAGTTCTC AAACAATCAC AATTGGTGTT TCGGAAATTT TAAACCTCCT TAGGACACTC 
GGAGAAGGGT GTAGACTTTC ATACATGTAC AGGTGTCAGG AGGCACTGGA TACGTATATG 
AAACTTCCAC ATAAGCATTA TAATACAGGA TGGGTTCTTT CCCAGGTCGG GAAAGCATAC 
TTTGAACTAA TTGACTATTT AGAGGCTGAA AAGGCATTCC GTCTTGCCCG TCTGGCTTCT 
CCTTATTGCT TAGAAGGAAT GGATATATAC TCTACGGTCC TCTATCATTT GAAGGAAGAC 
ATGAAGCTGA GTTACTTGGC TCAGGAACTA ATATCAACCG ATCGCTTAGC TCCTCAATCT 
TGGTGTGCTA TGGGAAATTG CTATAGCTTG CAAAAGGACC ATGAGACCGC ACTGAAGAAT 
TTCCTACGAG CTGTTCAACT GAATCCAAGA TTTGCATATG CACATACCTT ATGTGGCCAC 
GAATACACAA CTCTTGAGGA TTTTGAGAAC GGAATGAAAA GTTACCAAAA CGCACTTCGT 
GTAGATACAA GACACTACAA CGCATGGTAC GGGCTTGGAA TGATATATCT ACGCCAAGAG 
AAGTTAGAGT TCTCAGAGCA TCACTTCAGA ATGGCTTTCC TAATAAACCC GAGTTCCTCT 
GTTATAATGT CTTATTTAGG GACATCTTTG CATGCCTTGA AGGTTATCTT ATTACTTTCA 
TCTTATCAGG TCTACAAGAA AAACAATCTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Nature402(6763)761-8(1999 Dec)
Lin X,Kaul S,Rounsley S,Shea TP,Benito MI,Town CD,Fujii CY,Mason T,Bowman CL,Barnstead M,Feldblyum TV,Buell CR,Ketchum KA,Lee J,Ronning CM,Koo HL,Moffat KS,Cronin LA,Shen M,Pai G,Van Aken S,Umayam L,Tallon LJ,Gill JE,Adams MD,Carrera AJ,Creasy TH,Goodman HM,Somerville CR,Copenhaver GP,Preuss D,Nierman WC,White O,Eisen JA,Salzberg SL,Fraser CM,Venter JC

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HBT cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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