PHYB cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	PHYB
Entrez Gene ID	816394
Full Name	phytochrome B
Synonyms	HY3,MSF3.17,MSF3_17,OOP1,OUT OF PHASE 1,PHYTOCHROME B,phytochrome B
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001335612.1	NP_001325249.1	phytochrome B
NM_127435.4	NP_179469.1	phytochrome B

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	PHYB	NP_179469.1
Arabidopsis thaliana (thale cress)	PHYD	NP_193360.1
Oryza sativa (rice)	Os03g0309200	NP_001049910.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Lin X, Kaul S, Rounsley S, Shea TP, Benito MI, Town CD, Fujii CY, Mason T, Bowman CL, Barnstead M, Feldblyum TV, Buell CR, Ketchum KA, Lee J, Ronning CM, Koo HL, Moffat KS, Cronin LA, Shen M, Pai G, Van Aken S, Umayam L, Tallon LJ, Gill JE, Adams MD, Carrera AJ, Creasy TH, Goodman HM, Somerville CR, Copenhaver GP, Preuss D, Nierman WC, White O, Eisen JA, Salzberg SL, Fraser CM, Venter JC
Nature402(6763)761-8(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Genetic evidence indicated that HDA15 acts downstream of PHYB and represses seed germination in the dark dependent on PIF1.
Title: Identification of HDA15-PIF1 as a key repression module directing the transcriptional network of seed germination in the dark.

a null mutation of the JAZ10 gene in Arabidopsis did not affect plant growth nor did it suppress the shade-avoidance responses elicited by phyB inactivation.
Title: Exploring growth-defence trade-offs in Arabidopsis: phytochrome B inactivation requires JAZ10 to suppress plant immunity but not to trigger shade-avoidance responses.

The light-activated association of phyB, EIN3, and EBF1/EBF2 proteins stimulates robust EIN3 degradation by SCF(EBF1/EBF2) E3 ligases. We reveal that phyB manipulates substrate-E3 ligase interactions in a light-dependent manner, thus directly controlling the stability of EIN3.
Title: The Red Light Receptor Phytochrome B Directly Enhances Substrate-E3 Ligase Interactions to Attenuate Ethylene Responses.

Surprisingly, none of the phytochromes alone conferred a photoperiodic response. Although phyE and phyB were the strongest repressors of flowering, both phyB and phyC were needed to confer a flowering response to photoperiod. Thus, a specific combination of phytochromes is required to detect changes in photoperiod
Title: Bottom-up Assembly of the Phytochrome Network.

Light was efficiently conducted through the stems to the roots, where photoactivated phytochrome B (phyB) triggered expression of ELONGATED HYPOCOTYL 5 (HY5) and accumulation of HY5 protein, a transcription factor that promotes root growth in response to light.
Title: Stem-piped light activates phytochrome B to trigger light responses in Arabidopsis thaliana roots.

The following PHYB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHYB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb35785	NM_001335612.1 Latest version!	Arabidopsis thaliana phytochrome B (PHYB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAb07334	NM_127435.4 Latest version!	Arabidopsis thaliana phytochrome B (PHYB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PHYB ( NM_001335612.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001325249.1 Arabidopsis thaliana phytochrome B (PHYB), mRNA.

Price & Availability↑

CloneID	OAb35785
Clone ID Related Accession (Same CDS sequence)	NM_001335612.1
Accession Version	NM_001335612.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	phytochrome B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071).

ATGGTTTCCG GAGTCGGGGG TAGTGGCGGT GGCCGTGGCG GTGGCCGTGG CGGAGAAGAA 
GAACCGTCGT CAAGTCACAC TCCTAATAAC CGAAGAGGAG GAGAACAAGC TCAATCGTCG 
GGAACGAAAT CTCTCAGACC AAGAAGCAAC ACTGAATCAA TGAGCAAAGC AATTCAACAG 
TACACCGTCG ACGCAAGACT CCACGCCGTT TTCGAACAAT CCGGCGAATC AGGGAAATCA 
TTCGACTACT CACAATCACT CAAAACGACG ACGTACGGTT CCTCTGTACC TGAGCAACAG 
ATCACAGCTT ATCTCTCTCG AATCCAGCGA GGTGGTTACA TTCAGCCTTT CGGATGTATG 
ATCGCCGTCG ATGAATCCAG TTTCCGGATC ATCGGTTACA GTGAAAACGC CAGAGAAATG 
TTAGGGATTA TGCCTCAATC TGTTCCTACT CTTGAGAAAC CTGAGATTCT AGCTATGGGA 
ACTGATGTGA GATCTTTGTT CACTTCTTCG AGCTCGATTC TACTCGAGCG TGCTTTCGTT 
GCTCGAGAGA TTACCTTGTT AAATCCGGTT TGGATCCATT CCAAGAATAC TGGTAAACCG 
TTTTACGCCA TTCTTCATAG GATTGATGTT GGTGTTGTTA TTGATTTAGA GCCAGCTAGA 
ACTGAAGATC CTGCGCTTTC TATTGCTGGT GCTGTTCAAT CGCAGAAACT CGCGGTTCGT 
GCGATTTCTC AGTTACAGGC TCTTCCTGGT GGAGATATTA AGCTTTTGTG TGACACTGTC 
GTGGAAAGTG TGAGGGACTT GACTGGTTAT GATCGTGTTA TGGTTTATAA GTTTCATGAA 
GATGAGCATG GAGAAGTTGT AGCTGAGAGT AAACGAGATG ATTTAGAGCC TTATATTGGA 
CTGCATTATC CTGCTACTGA TATTCCTCAA GCGTCAAGGT TCTTGTTTAA GCAGAACCGT 
GTCCGAATGA TAGTAGATTG CAATGCCACA CCTGTTCTTG TGGTCCAGGA CGATAGGCTA 
ACTCAGTCTA TGTGCTTGGT TGGTTCTACT CTTAGGGCTC CTCATGGTTG TCACTCTCAG 
TATATGGCTA ACATGGGATC TATTGCGTCT TTAGCAATGG CGGTTATAAT CAATGGAAAT 
GAAGATGATG GGAGCAATGT AGCTAGTGGA AGAAGCTCGA TGAGGCTTTG GGGTTTGGTT 
GTTTGCCATC ACACTTCTTC TCGCTGCATA CCGTTTCCGC TAAGGTATGC TTGTGAGTTT 
TTGATGCAGG CTTTCGGTTT ACAGTTAAAC ATGGAATTGC AGTTAGCTTT GCAAATGTCA 
GAGAAACGCG TTTTGAGAAC GCAGACACTG TTATGTGATA TGCTTCTGCG TGACTCGCCT 
GCTGGAATTG TTACACAGAG TCCCAGTATC ATGGACTTAG TGAAATGTGA CGGTGCAGCA 
TTTCTTTACC ACGGGAAGTA TTACCCGTTG GGTGTTGCTC CTAGTGAAGT TCAGATAAAA 
GATGTTGTGG AGTGGTTGCT TGCGAATCAT GCGGATTCAA CCGGATTAAG CACTGATAGT 
TTAGGCGATG CGGGGTATCC CGGTGCAGCT GCGTTAGGGG ATGCTGTGTG CGGTATGGCA 
GTTGCATATA TCACAAAAAG AGACTTTCTT TTTTGGTTTC GATCTCACAC TGCGAAAGAA 
ATCAAATGGG GAGGCGCTAA GCATCATCCG GAGGATAAAG ATGATGGGCA ACGAATGCAT 
CCTCGTTCGT CCTTTCAGGC TTTTCTTGAA GTTGTTAAGA GCCGGAGTCA GCCATGGGAA 
ACTGCGGAAA TGGATGCGAT TCACTCGCTC CAGCTTATTC TGAGAGACTC TTTTAAAGAA 
TCTGAGGCGG CTATGAACTC TAAAGTTGTG GATGGTGTGG TTCAGCCATG TAGGGATATG 
GCGGGGGAAC AGGGGATTGA TGAGTTAGGT GCAGTTGCAA GAGAGATGGT TAGGCTCATT 
GAGACTGCAA CTGTTCCTAT ATTCGCTGTG GATGCCGGAG GCTGCATCAA TGGATGGAAC 
GCTAAGATTG CAGAGTTGAC AGGTCTCTCA GTTGAAGAAG CTATGGGGAA GTCTCTGGTT 
TCTGATTTAA TATACAAAGA GAATGAAGCA ACTGTCAATA AGCTTCTTTC TCGTGCTTTG 
AGAGGGGACG AGGAAAAGAA TGTGGAGGTT AAGCTGAAAA CTTTCAGCCC CGAACTACAA 
GGGAAAGCAG TTTTTGTGGT TGTGAATGCT TGTTCCAGCA AGGACTACTT GAACAACATT 
GTCGGCGTTT GTTTTGTTGG ACAAGACGTT ACTAGTCAGA AAATCGTAAT GGATAAGTTC 
ATCAACATAC AAGGAGATTA CAAGGCTATT GTACATAGCC CAAACCCTCT AATCCCGCCA 
ATTTTTGCTG CTGACGAGAA CACGTGCTGC CTGGAATGGA ACATGGCGAT GGAAAAGCTT 
ACGGGTTGGT CTCGCAGTGA AGTGATTGGG AAAATGATTG TCGGGGAAGT GTTTGGGAGC 
TGTTGCATGC TAAAGGGTCC TGATGCTTTA ACCAAGTTCA TGATTGTATT GCATAATGCG 
ATTGGTGGCC AAGATACGGA TAAGTTCCCT TTCCCATTCT TTGACCGCAA TGGGAAGTTT 
GTTCAGGCTC TATTGACTGC AAACAAGCGG GTTAGCCTCG AGGGAAAGGT TATTGGGGCT 
TTCTGTTTCT TGCAAATCCC GAGCCCTGAG CTGCAGCAAG CTTTAGCAGT CCAACGGAGG 
CAGGACACAG AGTGTTTCAC GAAGGCAAAA GAGTTGGCTT ATATTTGTCA GGTGATAAAG 
AATCCTTTGA GCGGTATGCG TTTCGCAAAC TCATTGTTGG AGGCCACAGA CTTGAACGAG 
GACCAGAAGC AGTTACTTGA AACAAGTGTT TCTTGCGAGA AACAGATCTC AAGGATCGTC 
GGGGACATGG ATCTTGAAAG CATTGAAGAC GGTTCATTTG TGCTAAAGAG GGAAGAGTTT 
TTCCTTGGAA GTGTCATAAA CGCGATTGTA AGTCAAGCGA TGTTCTTATT AAGGGACAGA 
GGTCTTCAGC TGATCCGTGA CATTCCCGAA GAGATCAAAT CAATAGAGGT TTTTGGAGAC 
CAGATAAGGA TTCAACAGCT CCTGGCTGAG TTTCTGCTGA GTATAATCCG GTATGCACCA 
TCTCAAGAGT GGGTGGAGAT CCATTTAAGC CAACTTTCAA AGCAAATGGC TGATGGATTC 
GCCGCCATCC GCACAGAATT CAGGTACATT TCATTGTTCC CGCTGTTGTC TCCACATATC 
CATAACCAAA ATTATGCAAT CCGGTTTTTT TGGTTCCTTA TTTTGTACAT AAAGAAAATG 
AATTTGGTTT GGTTAATTAC GAATTTGATT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001325249.1
CDS	324..3716
Translation	MVSGVGGSGGGRGGGRGGEEEPSSSHTPNNRRGGEQAQSSGTKSLRPRSNTESMSKAIQQYTVDARLHAVFEQSGESGKSFDYSQSLKTTTYGSSVPEQQITAYLSRIQRGGYIQPFGCMIAVDESSFRIIGYSENAREMLGIMPQSVPTLEKPEILAMGTDVRSLFTSSSSILLERAFVAREITLLNPVWIHSKNTGKPFYAILHRIDVGVVIDLEPARTEDPALSIAGAVQSQKLAVRAISQLQALPGGDIKLLCDTVVESVRDLTGYDRVMVYKFHEDEHGEVVAESKRDDLEPYIGLHYPATDIPQASRFLFKQNRVRMIVDCNATPVLVVQDDRLTQSMCLVGSTLRAPHGCHSQYMANMGSIASLAMAVIINGNEDDGSNVASGRSSMRLWGLVVCHHTSSRCIPFPLRYACEFLMQAFGLQLNMELQLALQMSEKRVLRTQTLLCDMLLRDSPAGIVTQSPSIMDLVKCDGAAFLYHGKYYPLGVAPSEVQIKDVVEWLLANHADSTGLSTDSLGDAGYPGAAALGDAVCGMAVAYITKRDFLFWFRSHTAKEIKWGGAKHHPEDKDDGQRMHPRSSFQAFLEVVKSRSQPWETAEMDAIHSLQLILRDSFKESEAAMNSKVVDGVVQPCRDMAGEQGIDELGAVAREMVRLIETATVPIFAVDAGGCINGWNAKIAELTGLSVEEAMGKSLVSDLIYKENEATVNKLLSRALRGDEEKNVEVKLKTFSPELQGKAVFVVVNACSSKDYLNNIVGVCFVGQDVTSQKIVMDKFINIQGDYKAIVHSPNPLIPPIFAADENTCCLEWNMAMEKLTGWSRSEVIGKMIVGEVFGSCCMLKGPDALTKFMIVLHNAIGGQDTDKFPFPFFDRNGKFVQALLTANKRVSLEGKVIGAFCFLQIPSPELQQALAVQRRQDTECFTKAKELAYICQVIKNPLSGMRFANSLLEATDLNEDQKQLLETSVSCEKQISRIVGDMDLESIEDGSFVLKREEFFLGSVINAIVSQAMFLLRDRGLQLIRDIPEEIKSIEVFGDQIRIQQLLAEFLLSIIRYAPSQEWVEIHLSQLSKQMADGFAAIRTEFRYISLFPLLSPHIHNQNYAIRFFWFLILYIKKMNLVWLITNLI

Target ORF information:

RefSeq Version	NM_001335612.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana phytochrome B (PHYB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001335612.1

ORF Insert Sequence:

ATGGTTTCCG GAGTCGGGGG TAGTGGCGGT GGCCGTGGCG GTGGCCGTGG CGGAGAAGAA 
GAACCGTCGT CAAGTCACAC TCCTAATAAC CGAAGAGGAG GAGAACAAGC TCAATCGTCG 
GGAACGAAAT CTCTCAGACC AAGAAGCAAC ACTGAATCAA TGAGCAAAGC AATTCAACAG 
TACACCGTCG ACGCAAGACT CCACGCCGTT TTCGAACAAT CCGGCGAATC AGGGAAATCA 
TTCGACTACT CACAATCACT CAAAACGACG ACGTACGGTT CCTCTGTACC TGAGCAACAG 
ATCACAGCTT ATCTCTCTCG AATCCAGCGA GGTGGTTACA TTCAGCCTTT CGGATGTATG 
ATCGCCGTCG ATGAATCCAG TTTCCGGATC ATCGGTTACA GTGAAAACGC CAGAGAAATG 
TTAGGGATTA TGCCTCAATC TGTTCCTACT CTTGAGAAAC CTGAGATTCT AGCTATGGGA 
ACTGATGTGA GATCTTTGTT CACTTCTTCG AGCTCGATTC TACTCGAGCG TGCTTTCGTT 
GCTCGAGAGA TTACCTTGTT AAATCCGGTT TGGATCCATT CCAAGAATAC TGGTAAACCG 
TTTTACGCCA TTCTTCATAG GATTGATGTT GGTGTTGTTA TTGATTTAGA GCCAGCTAGA 
ACTGAAGATC CTGCGCTTTC TATTGCTGGT GCTGTTCAAT CGCAGAAACT CGCGGTTCGT 
GCGATTTCTC AGTTACAGGC TCTTCCTGGT GGAGATATTA AGCTTTTGTG TGACACTGTC 
GTGGAAAGTG TGAGGGACTT GACTGGTTAT GATCGTGTTA TGGTTTATAA GTTTCATGAA 
GATGAGCATG GAGAAGTTGT AGCTGAGAGT AAACGAGATG ATTTAGAGCC TTATATTGGA 
CTGCATTATC CTGCTACTGA TATTCCTCAA GCGTCAAGGT TCTTGTTTAA GCAGAACCGT 
GTCCGAATGA TAGTAGATTG CAATGCCACA CCTGTTCTTG TGGTCCAGGA CGATAGGCTA 
ACTCAGTCTA TGTGCTTGGT TGGTTCTACT CTTAGGGCTC CTCATGGTTG TCACTCTCAG 
TATATGGCTA ACATGGGATC TATTGCGTCT TTAGCAATGG CGGTTATAAT CAATGGAAAT 
GAAGATGATG GGAGCAATGT AGCTAGTGGA AGAAGCTCGA TGAGGCTTTG GGGTTTGGTT 
GTTTGCCATC ACACTTCTTC TCGCTGCATA CCGTTTCCGC TAAGGTATGC TTGTGAGTTT 
TTGATGCAGG CTTTCGGTTT ACAGTTAAAC ATGGAATTGC AGTTAGCTTT GCAAATGTCA 
GAGAAACGCG TTTTGAGAAC GCAGACACTG TTATGTGATA TGCTTCTGCG TGACTCGCCT 
GCTGGAATTG TTACACAGAG TCCCAGTATC ATGGACTTAG TGAAATGTGA CGGTGCAGCA 
TTTCTTTACC ACGGGAAGTA TTACCCGTTG GGTGTTGCTC CTAGTGAAGT TCAGATAAAA 
GATGTTGTGG AGTGGTTGCT TGCGAATCAT GCGGATTCAA CCGGATTAAG CACTGATAGT 
TTAGGCGATG CGGGGTATCC CGGTGCAGCT GCGTTAGGGG ATGCTGTGTG CGGTATGGCA 
GTTGCATATA TCACAAAAAG AGACTTTCTT TTTTGGTTTC GATCTCACAC TGCGAAAGAA 
ATCAAATGGG GAGGCGCTAA GCATCATCCG GAGGATAAAG ATGATGGGCA ACGAATGCAT 
CCTCGTTCGT CCTTTCAGGC TTTTCTTGAA GTTGTTAAGA GCCGGAGTCA GCCATGGGAA 
ACTGCGGAAA TGGATGCGAT TCACTCGCTC CAGCTTATTC TGAGAGACTC TTTTAAAGAA 
TCTGAGGCGG CTATGAACTC TAAAGTTGTG GATGGTGTGG TTCAGCCATG TAGGGATATG 
GCGGGGGAAC AGGGGATTGA TGAGTTAGGT GCAGTTGCAA GAGAGATGGT TAGGCTCATT 
GAGACTGCAA CTGTTCCTAT ATTCGCTGTG GATGCCGGAG GCTGCATCAA TGGATGGAAC 
GCTAAGATTG CAGAGTTGAC AGGTCTCTCA GTTGAAGAAG CTATGGGGAA GTCTCTGGTT 
TCTGATTTAA TATACAAAGA GAATGAAGCA ACTGTCAATA AGCTTCTTTC TCGTGCTTTG 
AGAGGGGACG AGGAAAAGAA TGTGGAGGTT AAGCTGAAAA CTTTCAGCCC CGAACTACAA 
GGGAAAGCAG TTTTTGTGGT TGTGAATGCT TGTTCCAGCA AGGACTACTT GAACAACATT 
GTCGGCGTTT GTTTTGTTGG ACAAGACGTT ACTAGTCAGA AAATCGTAAT GGATAAGTTC 
ATCAACATAC AAGGAGATTA CAAGGCTATT GTACATAGCC CAAACCCTCT AATCCCGCCA 
ATTTTTGCTG CTGACGAGAA CACGTGCTGC CTGGAATGGA ACATGGCGAT GGAAAAGCTT 
ACGGGTTGGT CTCGCAGTGA AGTGATTGGG AAAATGATTG TCGGGGAAGT GTTTGGGAGC 
TGTTGCATGC TAAAGGGTCC TGATGCTTTA ACCAAGTTCA TGATTGTATT GCATAATGCG 
ATTGGTGGCC AAGATACGGA TAAGTTCCCT TTCCCATTCT TTGACCGCAA TGGGAAGTTT 
GTTCAGGCTC TATTGACTGC AAACAAGCGG GTTAGCCTCG AGGGAAAGGT TATTGGGGCT 
TTCTGTTTCT TGCAAATCCC GAGCCCTGAG CTGCAGCAAG CTTTAGCAGT CCAACGGAGG 
CAGGACACAG AGTGTTTCAC GAAGGCAAAA GAGTTGGCTT ATATTTGTCA GGTGATAAAG 
AATCCTTTGA GCGGTATGCG TTTCGCAAAC TCATTGTTGG AGGCCACAGA CTTGAACGAG 
GACCAGAAGC AGTTACTTGA AACAAGTGTT TCTTGCGAGA AACAGATCTC AAGGATCGTC 
GGGGACATGG ATCTTGAAAG CATTGAAGAC GGTTCATTTG TGCTAAAGAG GGAAGAGTTT 
TTCCTTGGAA GTGTCATAAA CGCGATTGTA AGTCAAGCGA TGTTCTTATT AAGGGACAGA 
GGTCTTCAGC TGATCCGTGA CATTCCCGAA GAGATCAAAT CAATAGAGGT TTTTGGAGAC 
CAGATAAGGA TTCAACAGCT CCTGGCTGAG TTTCTGCTGA GTATAATCCG GTATGCACCA 
TCTCAAGAGT GGGTGGAGAT CCATTTAAGC CAACTTTCAA AGCAAATGGC TGATGGATTC 
GCCGCCATCC GCACAGAATT CAGGTACATT TCATTGTTCC CGCTGTTGTC TCCACATATC 
CATAACCAAA ATTATGCAAT CCGGTTTTTT TGGTTCCTTA TTTTGTACAT AAAGAAAATG 
AATTTGGTTT GGTTAATTAC GAATTTGATT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHYB ( NM_127435.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_179469.1 Arabidopsis thaliana phytochrome B (PHYB), mRNA.

Price & Availability↑

CloneID	OAb07334
Clone ID Related Accession (Same CDS sequence)	NM_127435.4
Accession Version	NM_127435.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	phytochrome B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). On Sep 12, 2016 this sequence version replaced NM_127435.3.

ATGGTTTCCG GAGTCGGGGG TAGTGGCGGT GGCCGTGGCG GTGGCCGTGG CGGAGAAGAA 
GAACCGTCGT CAAGTCACAC TCCTAATAAC CGAAGAGGAG GAGAACAAGC TCAATCGTCG 
GGAACGAAAT CTCTCAGACC AAGAAGCAAC ACTGAATCAA TGAGCAAAGC AATTCAACAG 
TACACCGTCG ACGCAAGACT CCACGCCGTT TTCGAACAAT CCGGCGAATC AGGGAAATCA 
TTCGACTACT CACAATCACT CAAAACGACG ACGTACGGTT CCTCTGTACC TGAGCAACAG 
ATCACAGCTT ATCTCTCTCG AATCCAGCGA GGTGGTTACA TTCAGCCTTT CGGATGTATG 
ATCGCCGTCG ATGAATCCAG TTTCCGGATC ATCGGTTACA GTGAAAACGC CAGAGAAATG 
TTAGGGATTA TGCCTCAATC TGTTCCTACT CTTGAGAAAC CTGAGATTCT AGCTATGGGA 
ACTGATGTGA GATCTTTGTT CACTTCTTCG AGCTCGATTC TACTCGAGCG TGCTTTCGTT 
GCTCGAGAGA TTACCTTGTT AAATCCGGTT TGGATCCATT CCAAGAATAC TGGTAAACCG 
TTTTACGCCA TTCTTCATAG GATTGATGTT GGTGTTGTTA TTGATTTAGA GCCAGCTAGA 
ACTGAAGATC CTGCGCTTTC TATTGCTGGT GCTGTTCAAT CGCAGAAACT CGCGGTTCGT 
GCGATTTCTC AGTTACAGGC TCTTCCTGGT GGAGATATTA AGCTTTTGTG TGACACTGTC 
GTGGAAAGTG TGAGGGACTT GACTGGTTAT GATCGTGTTA TGGTTTATAA GTTTCATGAA 
GATGAGCATG GAGAAGTTGT AGCTGAGAGT AAACGAGATG ATTTAGAGCC TTATATTGGA 
CTGCATTATC CTGCTACTGA TATTCCTCAA GCGTCAAGGT TCTTGTTTAA GCAGAACCGT 
GTCCGAATGA TAGTAGATTG CAATGCCACA CCTGTTCTTG TGGTCCAGGA CGATAGGCTA 
ACTCAGTCTA TGTGCTTGGT TGGTTCTACT CTTAGGGCTC CTCATGGTTG TCACTCTCAG 
TATATGGCTA ACATGGGATC TATTGCGTCT TTAGCAATGG CGGTTATAAT CAATGGAAAT 
GAAGATGATG GGAGCAATGT AGCTAGTGGA AGAAGCTCGA TGAGGCTTTG GGGTTTGGTT 
GTTTGCCATC ACACTTCTTC TCGCTGCATA CCGTTTCCGC TAAGGTATGC TTGTGAGTTT 
TTGATGCAGG CTTTCGGTTT ACAGTTAAAC ATGGAATTGC AGTTAGCTTT GCAAATGTCA 
GAGAAACGCG TTTTGAGAAC GCAGACACTG TTATGTGATA TGCTTCTGCG TGACTCGCCT 
GCTGGAATTG TTACACAGAG TCCCAGTATC ATGGACTTAG TGAAATGTGA CGGTGCAGCA 
TTTCTTTACC ACGGGAAGTA TTACCCGTTG GGTGTTGCTC CTAGTGAAGT TCAGATAAAA 
GATGTTGTGG AGTGGTTGCT TGCGAATCAT GCGGATTCAA CCGGATTAAG CACTGATAGT 
TTAGGCGATG CGGGGTATCC CGGTGCAGCT GCGTTAGGGG ATGCTGTGTG CGGTATGGCA 
GTTGCATATA TCACAAAAAG AGACTTTCTT TTTTGGTTTC GATCTCACAC TGCGAAAGAA 
ATCAAATGGG GAGGCGCTAA GCATCATCCG GAGGATAAAG ATGATGGGCA ACGAATGCAT 
CCTCGTTCGT CCTTTCAGGC TTTTCTTGAA GTTGTTAAGA GCCGGAGTCA GCCATGGGAA 
ACTGCGGAAA TGGATGCGAT TCACTCGCTC CAGCTTATTC TGAGAGACTC TTTTAAAGAA 
TCTGAGGCGG CTATGAACTC TAAAGTTGTG GATGGTGTGG TTCAGCCATG TAGGGATATG 
GCGGGGGAAC AGGGGATTGA TGAGTTAGGT GCAGTTGCAA GAGAGATGGT TAGGCTCATT 
GAGACTGCAA CTGTTCCTAT ATTCGCTGTG GATGCCGGAG GCTGCATCAA TGGATGGAAC 
GCTAAGATTG CAGAGTTGAC AGGTCTCTCA GTTGAAGAAG CTATGGGGAA GTCTCTGGTT 
TCTGATTTAA TATACAAAGA GAATGAAGCA ACTGTCAATA AGCTTCTTTC TCGTGCTTTG 
AGAGGGGACG AGGAAAAGAA TGTGGAGGTT AAGCTGAAAA CTTTCAGCCC CGAACTACAA 
GGGAAAGCAG TTTTTGTGGT TGTGAATGCT TGTTCCAGCA AGGACTACTT GAACAACATT 
GTCGGCGTTT GTTTTGTTGG ACAAGACGTT ACTAGTCAGA AAATCGTAAT GGATAAGTTC 
ATCAACATAC AAGGAGATTA CAAGGCTATT GTACATAGCC CAAACCCTCT AATCCCGCCA 
ATTTTTGCTG CTGACGAGAA CACGTGCTGC CTGGAATGGA ACATGGCGAT GGAAAAGCTT 
ACGGGTTGGT CTCGCAGTGA AGTGATTGGG AAAATGATTG TCGGGGAAGT GTTTGGGAGC 
TGTTGCATGC TAAAGGGTCC TGATGCTTTA ACCAAGTTCA TGATTGTATT GCATAATGCG 
ATTGGTGGCC AAGATACGGA TAAGTTCCCT TTCCCATTCT TTGACCGCAA TGGGAAGTTT 
GTTCAGGCTC TATTGACTGC AAACAAGCGG GTTAGCCTCG AGGGAAAGGT TATTGGGGCT 
TTCTGTTTCT TGCAAATCCC GAGCCCTGAG CTGCAGCAAG CTTTAGCAGT CCAACGGAGG 
CAGGACACAG AGTGTTTCAC GAAGGCAAAA GAGTTGGCTT ATATTTGTCA GGTGATAAAG 
AATCCTTTGA GCGGTATGCG TTTCGCAAAC TCATTGTTGG AGGCCACAGA CTTGAACGAG 
GACCAGAAGC AGTTACTTGA AACAAGTGTT TCTTGCGAGA AACAGATCTC AAGGATCGTC 
GGGGACATGG ATCTTGAAAG CATTGAAGAC GGTTCATTTG TGCTAAAGAG GGAAGAGTTT 
TTCCTTGGAA GTGTCATAAA CGCGATTGTA AGTCAAGCGA TGTTCTTATT AAGGGACAGA 
GGTCTTCAGC TGATCCGTGA CATTCCCGAA GAGATCAAAT CAATAGAGGT TTTTGGAGAC 
CAGATAAGGA TTCAACAGCT CCTGGCTGAG TTTCTGCTGA GTATAATCCG GTATGCACCA 
TCTCAAGAGT GGGTGGAGAT CCATTTAAGC CAACTTTCAA AGCAAATGGC TGATGGATTC 
GCCGCCATCC GCACAGAATT CAGAATGGCG TGTCCAGGTG AAGGTCTGCC TCCAGAGCTA 
GTCCGAGACA TGTTCCATAG CAGCAGGTGG ACAAGCCCTG AAGGTTTAGG TCTAAGCGTA 
TGTCGAAAGA TTTTAAAGCT AATGAACGGT GAGGTTCAAT ACATCCGAGA ATCAGAACGG 
TCCTATTTCC TCATCATTCT GGAACTCCCT GTACCTCGAA AGCGACCATT GTCAACTGCT 
AGTGGAAGTG GTGACATGAT GCTGATGATG CCATATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_179469.1
CDS	317..3835
Translation	MVSGVGGSGGGRGGGRGGEEEPSSSHTPNNRRGGEQAQSSGTKSLRPRSNTESMSKAIQQYTVDARLHAVFEQSGESGKSFDYSQSLKTTTYGSSVPEQQITAYLSRIQRGGYIQPFGCMIAVDESSFRIIGYSENAREMLGIMPQSVPTLEKPEILAMGTDVRSLFTSSSSILLERAFVAREITLLNPVWIHSKNTGKPFYAILHRIDVGVVIDLEPARTEDPALSIAGAVQSQKLAVRAISQLQALPGGDIKLLCDTVVESVRDLTGYDRVMVYKFHEDEHGEVVAESKRDDLEPYIGLHYPATDIPQASRFLFKQNRVRMIVDCNATPVLVVQDDRLTQSMCLVGSTLRAPHGCHSQYMANMGSIASLAMAVIINGNEDDGSNVASGRSSMRLWGLVVCHHTSSRCIPFPLRYACEFLMQAFGLQLNMELQLALQMSEKRVLRTQTLLCDMLLRDSPAGIVTQSPSIMDLVKCDGAAFLYHGKYYPLGVAPSEVQIKDVVEWLLANHADSTGLSTDSLGDAGYPGAAALGDAVCGMAVAYITKRDFLFWFRSHTAKEIKWGGAKHHPEDKDDGQRMHPRSSFQAFLEVVKSRSQPWETAEMDAIHSLQLILRDSFKESEAAMNSKVVDGVVQPCRDMAGEQGIDELGAVAREMVRLIETATVPIFAVDAGGCINGWNAKIAELTGLSVEEAMGKSLVSDLIYKENEATVNKLLSRALRGDEEKNVEVKLKTFSPELQGKAVFVVVNACSSKDYLNNIVGVCFVGQDVTSQKIVMDKFINIQGDYKAIVHSPNPLIPPIFAADENTCCLEWNMAMEKLTGWSRSEVIGKMIVGEVFGSCCMLKGPDALTKFMIVLHNAIGGQDTDKFPFPFFDRNGKFVQALLTANKRVSLEGKVIGAFCFLQIPSPELQQALAVQRRQDTECFTKAKELAYICQVIKNPLSGMRFANSLLEATDLNEDQKQLLETSVSCEKQISRIVGDMDLESIEDGSFVLKREEFFLGSVINAIVSQAMFLLRDRGLQLIRDIPEEIKSIEVFGDQIRIQQLLAEFLLSIIRYAPSQEWVEIHLSQLSKQMADGFAAIRTEFRMACPGEGLPPELVRDMFHSSRWTSPEGLGLSVCRKILKLMNGEVQYIRESERSYFLIILELPVPRKRPLSTASGSGDMMLMMPY

Target ORF information:

RefSeq Version	NM_127435.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana phytochrome B (PHYB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_127435.4

ORF Insert Sequence:

ATGGTTTCCG GAGTCGGGGG TAGTGGCGGT GGCCGTGGCG GTGGCCGTGG CGGAGAAGAA 
GAACCGTCGT CAAGTCACAC TCCTAATAAC CGAAGAGGAG GAGAACAAGC TCAATCGTCG 
GGAACGAAAT CTCTCAGACC AAGAAGCAAC ACTGAATCAA TGAGCAAAGC AATTCAACAG 
TACACCGTCG ACGCAAGACT CCACGCCGTT TTCGAACAAT CCGGCGAATC AGGGAAATCA 
TTCGACTACT CACAATCACT CAAAACGACG ACGTACGGTT CCTCTGTACC TGAGCAACAG 
ATCACAGCTT ATCTCTCTCG AATCCAGCGA GGTGGTTACA TTCAGCCTTT CGGATGTATG 
ATCGCCGTCG ATGAATCCAG TTTCCGGATC ATCGGTTACA GTGAAAACGC CAGAGAAATG 
TTAGGGATTA TGCCTCAATC TGTTCCTACT CTTGAGAAAC CTGAGATTCT AGCTATGGGA 
ACTGATGTGA GATCTTTGTT CACTTCTTCG AGCTCGATTC TACTCGAGCG TGCTTTCGTT 
GCTCGAGAGA TTACCTTGTT AAATCCGGTT TGGATCCATT CCAAGAATAC TGGTAAACCG 
TTTTACGCCA TTCTTCATAG GATTGATGTT GGTGTTGTTA TTGATTTAGA GCCAGCTAGA 
ACTGAAGATC CTGCGCTTTC TATTGCTGGT GCTGTTCAAT CGCAGAAACT CGCGGTTCGT 
GCGATTTCTC AGTTACAGGC TCTTCCTGGT GGAGATATTA AGCTTTTGTG TGACACTGTC 
GTGGAAAGTG TGAGGGACTT GACTGGTTAT GATCGTGTTA TGGTTTATAA GTTTCATGAA 
GATGAGCATG GAGAAGTTGT AGCTGAGAGT AAACGAGATG ATTTAGAGCC TTATATTGGA 
CTGCATTATC CTGCTACTGA TATTCCTCAA GCGTCAAGGT TCTTGTTTAA GCAGAACCGT 
GTCCGAATGA TAGTAGATTG CAATGCCACA CCTGTTCTTG TGGTCCAGGA CGATAGGCTA 
ACTCAGTCTA TGTGCTTGGT TGGTTCTACT CTTAGGGCTC CTCATGGTTG TCACTCTCAG 
TATATGGCTA ACATGGGATC TATTGCGTCT TTAGCAATGG CGGTTATAAT CAATGGAAAT 
GAAGATGATG GGAGCAATGT AGCTAGTGGA AGAAGCTCGA TGAGGCTTTG GGGTTTGGTT 
GTTTGCCATC ACACTTCTTC TCGCTGCATA CCGTTTCCGC TAAGGTATGC TTGTGAGTTT 
TTGATGCAGG CTTTCGGTTT ACAGTTAAAC ATGGAATTGC AGTTAGCTTT GCAAATGTCA 
GAGAAACGCG TTTTGAGAAC GCAGACACTG TTATGTGATA TGCTTCTGCG TGACTCGCCT 
GCTGGAATTG TTACACAGAG TCCCAGTATC ATGGACTTAG TGAAATGTGA CGGTGCAGCA 
TTTCTTTACC ACGGGAAGTA TTACCCGTTG GGTGTTGCTC CTAGTGAAGT TCAGATAAAA 
GATGTTGTGG AGTGGTTGCT TGCGAATCAT GCGGATTCAA CCGGATTAAG CACTGATAGT 
TTAGGCGATG CGGGGTATCC CGGTGCAGCT GCGTTAGGGG ATGCTGTGTG CGGTATGGCA 
GTTGCATATA TCACAAAAAG AGACTTTCTT TTTTGGTTTC GATCTCACAC TGCGAAAGAA 
ATCAAATGGG GAGGCGCTAA GCATCATCCG GAGGATAAAG ATGATGGGCA ACGAATGCAT 
CCTCGTTCGT CCTTTCAGGC TTTTCTTGAA GTTGTTAAGA GCCGGAGTCA GCCATGGGAA 
ACTGCGGAAA TGGATGCGAT TCACTCGCTC CAGCTTATTC TGAGAGACTC TTTTAAAGAA 
TCTGAGGCGG CTATGAACTC TAAAGTTGTG GATGGTGTGG TTCAGCCATG TAGGGATATG 
GCGGGGGAAC AGGGGATTGA TGAGTTAGGT GCAGTTGCAA GAGAGATGGT TAGGCTCATT 
GAGACTGCAA CTGTTCCTAT ATTCGCTGTG GATGCCGGAG GCTGCATCAA TGGATGGAAC 
GCTAAGATTG CAGAGTTGAC AGGTCTCTCA GTTGAAGAAG CTATGGGGAA GTCTCTGGTT 
TCTGATTTAA TATACAAAGA GAATGAAGCA ACTGTCAATA AGCTTCTTTC TCGTGCTTTG 
AGAGGGGACG AGGAAAAGAA TGTGGAGGTT AAGCTGAAAA CTTTCAGCCC CGAACTACAA 
GGGAAAGCAG TTTTTGTGGT TGTGAATGCT TGTTCCAGCA AGGACTACTT GAACAACATT 
GTCGGCGTTT GTTTTGTTGG ACAAGACGTT ACTAGTCAGA AAATCGTAAT GGATAAGTTC 
ATCAACATAC AAGGAGATTA CAAGGCTATT GTACATAGCC CAAACCCTCT AATCCCGCCA 
ATTTTTGCTG CTGACGAGAA CACGTGCTGC CTGGAATGGA ACATGGCGAT GGAAAAGCTT 
ACGGGTTGGT CTCGCAGTGA AGTGATTGGG AAAATGATTG TCGGGGAAGT GTTTGGGAGC 
TGTTGCATGC TAAAGGGTCC TGATGCTTTA ACCAAGTTCA TGATTGTATT GCATAATGCG 
ATTGGTGGCC AAGATACGGA TAAGTTCCCT TTCCCATTCT TTGACCGCAA TGGGAAGTTT 
GTTCAGGCTC TATTGACTGC AAACAAGCGG GTTAGCCTCG AGGGAAAGGT TATTGGGGCT 
TTCTGTTTCT TGCAAATCCC GAGCCCTGAG CTGCAGCAAG CTTTAGCAGT CCAACGGAGG 
CAGGACACAG AGTGTTTCAC GAAGGCAAAA GAGTTGGCTT ATATTTGTCA GGTGATAAAG 
AATCCTTTGA GCGGTATGCG TTTCGCAAAC TCATTGTTGG AGGCCACAGA CTTGAACGAG 
GACCAGAAGC AGTTACTTGA AACAAGTGTT TCTTGCGAGA AACAGATCTC AAGGATCGTC 
GGGGACATGG ATCTTGAAAG CATTGAAGAC GGTTCATTTG TGCTAAAGAG GGAAGAGTTT 
TTCCTTGGAA GTGTCATAAA CGCGATTGTA AGTCAAGCGA TGTTCTTATT AAGGGACAGA 
GGTCTTCAGC TGATCCGTGA CATTCCCGAA GAGATCAAAT CAATAGAGGT TTTTGGAGAC 
CAGATAAGGA TTCAACAGCT CCTGGCTGAG TTTCTGCTGA GTATAATCCG GTATGCACCA 
TCTCAAGAGT GGGTGGAGAT CCATTTAAGC CAACTTTCAA AGCAAATGGC TGATGGATTC 
GCCGCCATCC GCACAGAATT CAGAATGGCG TGTCCAGGTG AAGGTCTGCC TCCAGAGCTA 
GTCCGAGACA TGTTCCATAG CAGCAGGTGG ACAAGCCCTG AAGGTTTAGG TCTAAGCGTA 
TGTCGAAAGA TTTTAAAGCT AATGAACGGT GAGGTTCAAT ACATCCGAGA ATCAGAACGG 
TCCTATTTCC TCATCATTCT GGAACTCCCT GTACCTCGAA AGCGACCATT GTCAACTGCT 
AGTGGAAGTG GTGACATGAT GCTGATGATG CCATATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Nature402(6763)761-8(1999 Dec)
Lin X,Kaul S,Rounsley S,Shea TP,Benito MI,Town CD,Fujii CY,Mason T,Bowman CL,Barnstead M,Feldblyum TV,Buell CR,Ketchum KA,Lee J,Ronning CM,Koo HL,Moffat KS,Cronin LA,Shen M,Pai G,Van Aken S,Umayam L,Tallon LJ,Gill JE,Adams MD,Carrera AJ,Creasy TH,Goodman HM,Somerville CR,Copenhaver GP,Preuss D,Nierman WC,White O,Eisen JA,Salzberg SL,Fraser CM,Venter JC

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PHYB cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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