MANBA cDNA ORF clone, Apteryx australis mantelli

Gene
Clones
gRNAs

Summary

Gene Symbol	MANBA
Entrez Gene ID	106491981
Full Name	mannosidase beta
Gene Type	protein-coding
Organism	Apteryx australis mantelli

mRNA and Protein(s)

mRNA	Protein	Name
XM_013951655.1	XP_013807109.1	beta-mannosidase

Pathways from BioSystems
Gene Ontology

The following MANBA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MANBA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAp20856	XM_013951655.1 Latest version!	Apteryx australis mantelli mannosidase, beta A, lysosomal (MANBA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MANBA ( XM_013951655.1 ) cDNA ORF clone, Apteryx australis mantelli -> XP_013807109.1 Apteryx australis mantelli mannosidase, beta A, lysosomal (MANBA), mRNA.

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CloneID	OAp20856
Clone ID Related Accession (Same CDS sequence)	XM_013951655.1
Accession Version	XM_013951655.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-08
Organism	Apteryx australis mantelli
Product	beta-mannosidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014000569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apteryx australis mantelli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGAT GGATCTCACT GGATAACTGG ACTTACAGCA GGACATTCAA AACTCCTTTT 
GACGTCAGAA AGTGGCAAAA AGTGAATTTG GTTTTTGAGG GAGTGGATAC AGTAGCACAA 
ATCCTGATCA ACAATATCAC TGTTGGCAGA ACAGACAACA TGTTCAGCAG ATATAGCTTT 
GATATTACCA GCATTATTAA GGAGGTCAAT TTTGTTGAAG TCCGTTTCTT ATCAGCCATT 
TCATACGCAG CAGAGCAGAG CAGATGCCAC ACAGCATACA GCATCCCCCC GGCGTGCCCT 
CCACCTGTGC AGAAAGGAGA GTGCCATGCT AATTTCATCC GAAAGGAGCA GTGTTCCTTT 
AGTTGGGACT GGGGCCCTTC TTTTCCCACT CAAGGAATCT GGAAGGATGT TAGGATTGAG 
GCCTATAACC ATTATCACCT GATTTACTTC ACTTTGACTC CCTTCTTTGT AAAGAGTGCT 
CAGCAGTGGA GTCTTGAAAT TGAGTCTATT TTTGATGTAG TCAGCTCCAA GCCGGTTTCA 
GGTCTTGTGA CTGTGAGCAT TCCCAAGTTG CAAACGCAGC AAACATACAG TGTGAAGCTT 
CAACCTGGAG AAGGCAGCAT TGTGCTGCTT GTAAACATCA ACGAGAATGC TACAGTAGAG 
ACTTGGTGGC CAAATGGACA TGGAAAACAA ACTGGATACA ACATGACGAC AGTTTTCATC 
ATGGAGGCAG GATACAAGAT TGAGAAGTTT TCAAAGGCCT ATTTTCGGAC AGTAGAACTT 
GTTCAGGAGC CTATTTCTGG GTCACCAGGT CTCAGTTTCT ACTTCAGAAT CAATGGCCGA 
CCCATTTTCA TCAAGGGCTC AAACTGGATA CCAGCAGATT CTTTCCAGGA CAGAGTGACC 
TATGACATAT TACGGCTACT CTTGAAGTCT GCAGCAGATG CTAACATGAA TGCACTCCGG 
GTCTGGGGAG GAGGAGTGTA TGAGCAAGAT GAATTTTATG ATATTTGTGA TGAGCTAGGA 
ATAATGATTT GGCAGGATTT TATGTTTGCT TGTGCACTTT ATCCAACTGA TCAGAATTAC 
TTGGAATCGA TAAGAGCAGA AGTTACTCAT CAGATCAGGC GGTTGAAATC TCATCCATCA 
ATTATTTTAT GGAGTGGCAA CAATGAAAAC GAAGCGGCGA TTGCAAGCAA CTGGTTCTCC 
ATACCTTATG CTGACAGAGA AGTCTATGTC AGAGATTATG TGATGCTTTA TGTGAAGAAC 
ATCCGAGAAA TAGTTCTTGC TGAAGATAAG AGTCGTCCTT TCATTGCATC CAGTCCCACA 
AATGGCTTGG AGTCAGTTAA AGAAGGTTGG CTCTCCCAAA ATCCTTACGA TACCCACTAT 
GGTGACACTC ACTTTTATGA CTACAGCAAT GACTGCTGGG ACTGGACATT GTATCCTAAA 
ACTCGTTTTG CTTCAGAATA TGGATTTCAG TCCTGGCCTT CCTTCAGTAC GCTGGAAAAG 
GTTTCTTCTG CAGAGGACTG GTCCTACACA AGCAATTTTT CTCTCCATCG ACAGCACCAT 
GAAAATGGCA ATGACGAAAT GATTCAACAA ATTGAGCGTC ATTTCAAGCT TCCCCAGAGC 
ACAAATCCCA TTAAAAAGTT CAAAGATATG ATTTACCTCA CTCAGGTGAT GCAGGCCCAG 
TGTGTAAAGA CAGAAACTGA ATTCTATCGT TTTAGTCAAA GTGAGATAAT CAAGGGAGAA 
GGACACACAA TGGGTGCGCT GTATTGGCAA CTCAATGACA TCTGGCAGGC ACCTTCATGG 
GCTTCCTTGG AGTATGGTGG TAAGTGGAAA CTGCTCCATT ATTTTGCCCA GAATTTCTTT 
GCACCACTGC TGCCTGTGGC TTATGAGGAT GGAGGTGCGT TGTACATCTA TGGTGTCTCA 
GATCTTCACA CAGACCGTCA GCTAACACTC GGGGTCACTG TGCACACATG GAGTTCTCTG 
GAGCCAGTGT GCAGCCTTGT CAAAGACGAC GTGACCGTTA AAGCACAAAG CGCTGTCTCC 
ATCTACAAGG AGCCCATAAG CGACCTGCTG AGAAGATGCA GAAACTGCAC CAGGAAAAGC 
TGTGTTGTTA CTTTTTACCT TGTGGGAGAA GGTGGGCTCC GCAGTCCTAC GAACCATCAC 
TTCCTGTCAT CACTAAAGGA TGCTGTGGGA TTGGAAAAGA CTCAGCTTAG TGCCAGTGTA 
TCCCAACAAG ACAGCATTTA TATATTTGAT CTTCAGACCA CTGCAATTGC TCCTTTTGTT 
TCTCTGGATG TAGGGAGTAT AAAAGGCAGA TTTAATGAAA ATGGTTTTCT CATGACCAAA 
AAGAGGAAAC TGGTTGTTTT CTACCCTTGG GAGCCTACCA CTGTTGAAGA ACTAGAAAAA 
TCTCTCAGCT TGACCTCTCT GATGGACGTC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013807109.1
CDS	24..2459
Translation	MYRWISLDNWTYSRTFKTPFDVRKWQKVNLVFEGVDTVAQILINNITVGRTDNMFSRYSFDITSIIKEVNFVEVRFLSAISYAAEQSRCHTAYSIPPACPPPVQKGECHANFIRKEQCSFSWDWGPSFPTQGIWKDVRIEAYNHYHLIYFTLTPFFVKSAQQWSLEIESIFDVVSSKPVSGLVTVSIPKLQTQQTYSVKLQPGEGSIVLLVNINENATVETWWPNGHGKQTGYNMTTVFIMEAGYKIEKFSKAYFRTVELVQEPISGSPGLSFYFRINGRPIFIKGSNWIPADSFQDRVTYDILRLLLKSAADANMNALRVWGGGVYEQDEFYDICDELGIMIWQDFMFACALYPTDQNYLESIRAEVTHQIRRLKSHPSIILWSGNNENEAAIASNWFSIPYADREVYVRDYVMLYVKNIREIVLAEDKSRPFIASSPTNGLESVKEGWLSQNPYDTHYGDTHFYDYSNDCWDWTLYPKTRFASEYGFQSWPSFSTLEKVSSAEDWSYTSNFSLHRQHHENGNDEMIQQIERHFKLPQSTNPIKKFKDMIYLTQVMQAQCVKTETEFYRFSQSEIIKGEGHTMGALYWQLNDIWQAPSWASLEYGGKWKLLHYFAQNFFAPLLPVAYEDGGALYIYGVSDLHTDRQLTLGVTVHTWSSLEPVCSLVKDDVTVKAQSAVSIYKEPISDLLRRCRNCTRKSCVVTFYLVGEGGLRSPTNHHFLSSLKDAVGLEKTQLSASVSQQDSIYIFDLQTTAIAPFVSLDVGSIKGRFNENGFLMTKKRKLVVFYPWEPTTVEELEKSLSLTSLMDVV

Target ORF information:

RefSeq Version	XM_013951655.1
Organism	Apteryx australis mantelli
Definition	Apteryx australis mantelli mannosidase, beta A, lysosomal (MANBA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013951655.1

ORF Insert Sequence:

ATGTACAGAT GGATCTCACT GGATAACTGG ACTTACAGCA GGACATTCAA AACTCCTTTT 
GACGTCAGAA AGTGGCAAAA AGTGAATTTG GTTTTTGAGG GAGTGGATAC AGTAGCACAA 
ATCCTGATCA ACAATATCAC TGTTGGCAGA ACAGACAACA TGTTCAGCAG ATATAGCTTT 
GATATTACCA GCATTATTAA GGAGGTCAAT TTTGTTGAAG TCCGTTTCTT ATCAGCCATT 
TCATACGCAG CAGAGCAGAG CAGATGCCAC ACAGCATACA GCATCCCCCC GGCGTGCCCT 
CCACCTGTGC AGAAAGGAGA GTGCCATGCT AATTTCATCC GAAAGGAGCA GTGTTCCTTT 
AGTTGGGACT GGGGCCCTTC TTTTCCCACT CAAGGAATCT GGAAGGATGT TAGGATTGAG 
GCCTATAACC ATTATCACCT GATTTACTTC ACTTTGACTC CCTTCTTTGT AAAGAGTGCT 
CAGCAGTGGA GTCTTGAAAT TGAGTCTATT TTTGATGTAG TCAGCTCCAA GCCGGTTTCA 
GGTCTTGTGA CTGTGAGCAT TCCCAAGTTG CAAACGCAGC AAACATACAG TGTGAAGCTT 
CAACCTGGAG AAGGCAGCAT TGTGCTGCTT GTAAACATCA ACGAGAATGC TACAGTAGAG 
ACTTGGTGGC CAAATGGACA TGGAAAACAA ACTGGATACA ACATGACGAC AGTTTTCATC 
ATGGAGGCAG GATACAAGAT TGAGAAGTTT TCAAAGGCCT ATTTTCGGAC AGTAGAACTT 
GTTCAGGAGC CTATTTCTGG GTCACCAGGT CTCAGTTTCT ACTTCAGAAT CAATGGCCGA 
CCCATTTTCA TCAAGGGCTC AAACTGGATA CCAGCAGATT CTTTCCAGGA CAGAGTGACC 
TATGACATAT TACGGCTACT CTTGAAGTCT GCAGCAGATG CTAACATGAA TGCACTCCGG 
GTCTGGGGAG GAGGAGTGTA TGAGCAAGAT GAATTTTATG ATATTTGTGA TGAGCTAGGA 
ATAATGATTT GGCAGGATTT TATGTTTGCT TGTGCACTTT ATCCAACTGA TCAGAATTAC 
TTGGAATCGA TAAGAGCAGA AGTTACTCAT CAGATCAGGC GGTTGAAATC TCATCCATCA 
ATTATTTTAT GGAGTGGCAA CAATGAAAAC GAAGCGGCGA TTGCAAGCAA CTGGTTCTCC 
ATACCTTATG CTGACAGAGA AGTCTATGTC AGAGATTATG TGATGCTTTA TGTGAAGAAC 
ATCCGAGAAA TAGTTCTTGC TGAAGATAAG AGTCGTCCTT TCATTGCATC CAGTCCCACA 
AATGGCTTGG AGTCAGTTAA AGAAGGTTGG CTCTCCCAAA ATCCTTACGA TACCCACTAT 
GGTGACACTC ACTTTTATGA CTACAGCAAT GACTGCTGGG ACTGGACATT GTATCCTAAA 
ACTCGTTTTG CTTCAGAATA TGGATTTCAG TCCTGGCCTT CCTTCAGTAC GCTGGAAAAG 
GTTTCTTCTG CAGAGGACTG GTCCTACACA AGCAATTTTT CTCTCCATCG ACAGCACCAT 
GAAAATGGCA ATGACGAAAT GATTCAACAA ATTGAGCGTC ATTTCAAGCT TCCCCAGAGC 
ACAAATCCCA TTAAAAAGTT CAAAGATATG ATTTACCTCA CTCAGGTGAT GCAGGCCCAG 
TGTGTAAAGA CAGAAACTGA ATTCTATCGT TTTAGTCAAA GTGAGATAAT CAAGGGAGAA 
GGACACACAA TGGGTGCGCT GTATTGGCAA CTCAATGACA TCTGGCAGGC ACCTTCATGG 
GCTTCCTTGG AGTATGGTGG TAAGTGGAAA CTGCTCCATT ATTTTGCCCA GAATTTCTTT 
GCACCACTGC TGCCTGTGGC TTATGAGGAT GGAGGTGCGT TGTACATCTA TGGTGTCTCA 
GATCTTCACA CAGACCGTCA GCTAACACTC GGGGTCACTG TGCACACATG GAGTTCTCTG 
GAGCCAGTGT GCAGCCTTGT CAAAGACGAC GTGACCGTTA AAGCACAAAG CGCTGTCTCC 
ATCTACAAGG AGCCCATAAG CGACCTGCTG AGAAGATGCA GAAACTGCAC CAGGAAAAGC 
TGTGTTGTTA CTTTTTACCT TGTGGGAGAA GGTGGGCTCC GCAGTCCTAC GAACCATCAC 
TTCCTGTCAT CACTAAAGGA TGCTGTGGGA TTGGAAAAGA CTCAGCTTAG TGCCAGTGTA 
TCCCAACAAG ACAGCATTTA TATATTTGAT CTTCAGACCA CTGCAATTGC TCCTTTTGTT 
TCTCTGGATG TAGGGAGTAT AAAAGGCAGA TTTAATGAAA ATGGTTTTCT CATGACCAAA 
AAGAGGAAAC TGGTTGTTTT CTACCCTTGG GAGCCTACCA CTGTTGAAGA ACTAGAAAAA 
TCTCTCAGCT TGACCTCTCT GATGGACGTC GTCTGA