ACLY cDNA ORF clone, Apteryx australis mantelli

Gene
Clones
gRNAs

Summary

Gene Symbol	ACLY
Entrez Gene ID	106487628
Full Name	ATP citrate lyase
Gene Type	protein-coding
Organism	Apteryx australis mantelli

mRNA and Protein(s)

mRNA	Protein	Name
XM_013946404.1	XP_013801858.1	ATP-citrate synthase isoform X1
XM_013946405.1	XP_013801859.1	ATP-citrate synthase isoform X2

Pathways from BioSystems
Gene Ontology

The following ACLY gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACLY cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAp16919	XM_013946404.1 Latest version!	Apteryx australis mantelli ATP citrate lyase (ACLY), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAp16920	XM_013946405.1 Latest version!	Apteryx australis mantelli ATP citrate lyase (ACLY), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACLY ( XM_013946404.1 ) cDNA ORF clone, Apteryx australis mantelli -> XP_013801858.1 Apteryx australis mantelli ATP citrate lyase (ACLY), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAp16919
Clone ID Related Accession (Same CDS sequence)	XM_013946404.1
Accession Version	XM_013946404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-08
Organism	Apteryx australis mantelli
Product	ATP-citrate synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013994976.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apteryx australis mantelli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGCCA AGGCCATCTC TGAACAGACT GGGAAGGAGT TCTTGTATAA GTATATCTGC 
ACATCCTCTG CTATCCAGAA CCGCTTCAAG TATGCCCGTG TCACCCCAGA CACTGACTGG 
GCGCGACTCA CCCAGGACCA CCCGTGGCTT CTGAGCGAGC GTCTAGTGGT GAAGCCAGAT 
CAGCTAATAA AACGGCGTGG GAAGCTTGGA CTGGTTGGAA TTAACTTCAC TCTAGATCAG 
GTGAAGGTTT GGCTGAAACA GCGTCTGGGC AAAGAAACCA CAATTGCTAA TGCTAAGGGA 
GTCCTGAAGA ATTTTCTGAT TGAGCCCTTC GTCCCCCACA GACAGGAAGA AGAGTTCTAT 
GTGTGCATAT ATGCTGCCCG CGAGGGAGAC TATGTACTCT TCCACCACGA AGGGGGTGTG 
GATGTGGGAG ATGTTGATGC CAAAGCTCAG AAGCTGCTCG TGGGGGTGGA TGAAAAGCTG 
AATGAATCTG ATGTAAAGAA ACATCTCCTG CAGCATGCAC CAGCAAATAA GAAAGATATC 
TTGGCTAGCT TCATCTCTGG CCTGTTCAAC CTTTATGAAG ATCTGTATTT CACGTACCTC 
GAGATCAATC CATTAGTTGT GACTCAAGAT GGTGTCTACA TCCTTGATTT GGCTGCGAAG 
ATTGATGCGA CTGCTGACTA CATCTGTAAA GTGAAATGGG GGGATGTGGA ATTCCCCCCT 
CCCTTTGGCA GGGAAGCCTA CCCAGAGGAA GCCTACATTG CTGACCTGGA TGCAAAGAGT 
GGAGCCAGCC TGAAACTTAC TATTCTCAAT CCCAAAGGCA GGATCTGGAC CATGGTGGCT 
GGTGGTGGTG CCTCTGTGGT TTACAGTGAC ACCATTTGTG ACCTGGGGGG TGTGAATGAA 
CTGGCTAACT ATGGTGAATA CTCAGGAGCC CCAAGTGAGC AACAAACTTA TGACTATGCT 
AAGACTATTC TCTCCCTCAT GACACGGGAG AAACACCCTG AAGGTAAAAT CCTAATCATT 
GGAGGCAGCA TCGCTAACTT CACCAATGTG GCAGCCACTT TTAAAGGCAT TGTGAGAGCA 
ATTAAAGACT ATCAAGGCCC TCTGAAGGAG CATGAAGTGA GGATCTTTGT GCGAAGGGGA 
GGCCCAAACT ACCAGGAAGG ACTGCGCGTC ATGGGAGAAG TGGGGAAGAC CACTGGGATC 
CCCATCCATG TCTTTGGCAC AGAGACTCAC ATGACTGCTA TTGTGGGCAT GGCACTTGGC 
CATCGGCCTA TCCCTAATCA GCCACCTGCC GCAGCTCACA CTGCCAACTT CCTCCTCAAC 
GCCAGCGGCA GCCCTTCGAC TCCAGCACCA AGCAGAACAG CTTCCTTCTC TGAGTCCAAA 
GCAGATGATA TTGCTCCAGC CAAGAAGGCA AAGCCAACAG CACCTCTTGA TTCAGTCCCA 
GCTTCAAGAC CTGGTCCAGG TAAAGCTACA ACCCTGTTCA GCCGTCACAC CAAAGCTATT 
ATTTGGGGGA TGCAGACACG GGCTGTGCAA GGAATGCTGG ACTTCGATTA TATTTGCTCC 
CGGGATGAAC CTTCAGTAGC TGCCATGGTT TATCCGTTCA CTGGTGATCA CAAGCAGAAG 
TTCTATTGGG GCCACAAAGA AATCCTGATC CCAGTCTACA AGAACATGTC TGATGCTATG 
AGGAAGCACC CTGAGGTGGA CGTTCTAATC AACTTTGCAT CTCTGCGGTC TGCTTATGAC 
AGCACTATTG AAACCATGAA CTATCCACAG ATCCGCACCA TTGCCATTAT TGCTGAGGGC 
ATTCCAGAGG CACTGACGCG CAAGCTGATC AAGACAGCTG ATAAGAAAGG AGTCACGATC 
ATTGGGCCTG CAACTGTTGG TGGGATCAAA CCAGGTTGCT TTAAAATAGG CAACACAGGA 
GGCATGCTGG ATAATATCTT AGCATCAAAA CTCTACCGTC CTGGCAGCGT GGCTTATGTC 
TCACGCTCTG GAGGAATGTC CAATGAGCTC AACAACATCA TTTCCCGAAC CACGGATGGG 
GTCTATGAAG GGGTGGCCAT TGGAGGAGAC AGATACCCTG GTTCAACTTT TATGGATCAT 
GTCTTACGCT ATGAGGATAC TCCAGGAGTG AAAATGATTG TAGTGCTTGG AGAGATTGGA 
GGCACAGAAG AGTATAAGAT CTCCAGGGGT ATTAAAGAAG GCCGTATTAC CAAGCCAGTA 
GTGTGCTGGT GTATTGGTAC CTGTGCCACC ATGTTCTCTT CAGAGGTACA GTTTGGCCAT 
GCAGGAGCTT GTGCCAACCA GGCTTCTGAA ACTGCTGTTG CAAAGAATCA GGCCTTGAAG 
GAAGCTGGGG TGTTTGTTCC CCGGAGTTTT GATGAGCTGG GAGAGGTCAT TCAGTCTGTC 
TATCAGGATC TTGTTGCCAA ACGAGTGATT GAACCAGCTG AGGAAGTGCC TCCTCCAACT 
GTGCCAATGG ATTATTCCTG GGCAAGGGAG CTGGGTCTGA TCCGCAAGCC AGCTTCCTTT 
ATGACCAGCA TCTGTGACGA GAGAGGTCAG GAACTGATTT ATGCTGGGAT GCCCATCACT 
GAGGTCTTCA AAGAAGAAAT GGGAATTGGA GGGGTTCTGG GACTGCTCTG GTTTCAAAGG 
AGGTTACCAA AGTATGCCTG TCAGTTTATC GAGATGTGTT TGATGGTAAC AGCAGATCAT 
GGGCCTGCTG TATCTGGAGC CCACAACACT ATTGTCTGCG CAAGAGCGGG AAAAGATCTT 
GTCTCAAGTC TCACTTCAGG CCTTCTTACT ATTGGTGACC GGTTTGGTGG GGCACTGGAT 
GCTGCAGCTA AAATGTTCAG CAAAGCCTTT GACAGTGGCA TTATCCCTAT GGAGTTTGTG 
AATAAAATGA AGAAGGAAGG GAAACTGATC ATGGGCATCG GACACAGGGT CAAATCGATA 
AATAATCCAG ACATGAGAGT TCAGATTCTT AAAGACTACG TGAAGCAGCA TTTCCCTGCC 
ACCCCACTGT TGGACTATGC ACTTGAAGTG GAAAAAATTA CAACTTCCAA GAAACCAAAT 
CTTATCCTGA ATGTAGATGG CTTTATTGGA GTTGCCTTTG TTGATGTACT CAGGAATTGT 
GGCTCTTTCA CACGGGAAGA AGCAGATGAA TATATTGATA TAGGAGCTCT TAATGGCATC 
TTCGTACTAG GCAGGAGTAT GGGATTCATT GGACACTACC TGGATCAGAA GAGGTTGAAG 
CAAGGTCTCT ACCGTCACCC ATGGGACGAC ATATCCTATG TTTTACCAGA GCACATGACT 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013801858.1
CDS	89..3394
Translation	MSAKAISEQTGKEFLYKYICTSSAIQNRFKYARVTPDTDWARLTQDHPWLLSERLVVKPDQLIKRRGKLGLVGINFTLDQVKVWLKQRLGKETTIANAKGVLKNFLIEPFVPHRQEEEFYVCIYAAREGDYVLFHHEGGVDVGDVDAKAQKLLVGVDEKLNESDVKKHLLQHAPANKKDILASFISGLFNLYEDLYFTYLEINPLVVTQDGVYILDLAAKIDATADYICKVKWGDVEFPPPFGREAYPEEAYIADLDAKSGASLKLTILNPKGRIWTMVAGGGASVVYSDTICDLGGVNELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPEGKILIIGGSIANFTNVAATFKGIVRAIKDYQGPLKEHEVRIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALGHRPIPNQPPAAAHTANFLLNASGSPSTPAPSRTASFSESKADDIAPAKKAKPTAPLDSVPASRPGPGKATTLFSRHTKAIIWGMQTRAVQGMLDFDYICSRDEPSVAAMVYPFTGDHKQKFYWGHKEILIPVYKNMSDAMRKHPEVDVLINFASLRSAYDSTIETMNYPQIRTIAIIAEGIPEALTRKLIKTADKKGVTIIGPATVGGIKPGCFKIGNTGGMLDNILASKLYRPGSVAYVSRSGGMSNELNNIISRTTDGVYEGVAIGGDRYPGSTFMDHVLRYEDTPGVKMIVVLGEIGGTEEYKISRGIKEGRITKPVVCWCIGTCATMFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGEVIQSVYQDLVAKRVIEPAEEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITEVFKEEMGIGGVLGLLWFQRRLPKYACQFIEMCLMVTADHGPAVSGAHNTIVCARAGKDLVSSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGIIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDYVKQHFPATPLLDYALEVEKITTSKKPNLILNVDGFIGVAFVDVLRNCGSFTREEADEYIDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMTM

Target ORF information:

RefSeq Version	XM_013946404.1
Organism	Apteryx australis mantelli
Definition	Apteryx australis mantelli ATP citrate lyase (ACLY), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013946404.1

ORF Insert Sequence:

ATGTCAGCCA AGGCCATCTC TGAACAGACT GGGAAGGAGT TCTTGTATAA GTATATCTGC 
ACATCCTCTG CTATCCAGAA CCGCTTCAAG TATGCCCGTG TCACCCCAGA CACTGACTGG 
GCGCGACTCA CCCAGGACCA CCCGTGGCTT CTGAGCGAGC GTCTAGTGGT GAAGCCAGAT 
CAGCTAATAA AACGGCGTGG GAAGCTTGGA CTGGTTGGAA TTAACTTCAC TCTAGATCAG 
GTGAAGGTTT GGCTGAAACA GCGTCTGGGC AAAGAAACCA CAATTGCTAA TGCTAAGGGA 
GTCCTGAAGA ATTTTCTGAT TGAGCCCTTC GTCCCCCACA GACAGGAAGA AGAGTTCTAT 
GTGTGCATAT ATGCTGCCCG CGAGGGAGAC TATGTACTCT TCCACCACGA AGGGGGTGTG 
GATGTGGGAG ATGTTGATGC CAAAGCTCAG AAGCTGCTCG TGGGGGTGGA TGAAAAGCTG 
AATGAATCTG ATGTAAAGAA ACATCTCCTG CAGCATGCAC CAGCAAATAA GAAAGATATC 
TTGGCTAGCT TCATCTCTGG CCTGTTCAAC CTTTATGAAG ATCTGTATTT CACGTACCTC 
GAGATCAATC CATTAGTTGT GACTCAAGAT GGTGTCTACA TCCTTGATTT GGCTGCGAAG 
ATTGATGCGA CTGCTGACTA CATCTGTAAA GTGAAATGGG GGGATGTGGA ATTCCCCCCT 
CCCTTTGGCA GGGAAGCCTA CCCAGAGGAA GCCTACATTG CTGACCTGGA TGCAAAGAGT 
GGAGCCAGCC TGAAACTTAC TATTCTCAAT CCCAAAGGCA GGATCTGGAC CATGGTGGCT 
GGTGGTGGTG CCTCTGTGGT TTACAGTGAC ACCATTTGTG ACCTGGGGGG TGTGAATGAA 
CTGGCTAACT ATGGTGAATA CTCAGGAGCC CCAAGTGAGC AACAAACTTA TGACTATGCT 
AAGACTATTC TCTCCCTCAT GACACGGGAG AAACACCCTG AAGGTAAAAT CCTAATCATT 
GGAGGCAGCA TCGCTAACTT CACCAATGTG GCAGCCACTT TTAAAGGCAT TGTGAGAGCA 
ATTAAAGACT ATCAAGGCCC TCTGAAGGAG CATGAAGTGA GGATCTTTGT GCGAAGGGGA 
GGCCCAAACT ACCAGGAAGG ACTGCGCGTC ATGGGAGAAG TGGGGAAGAC CACTGGGATC 
CCCATCCATG TCTTTGGCAC AGAGACTCAC ATGACTGCTA TTGTGGGCAT GGCACTTGGC 
CATCGGCCTA TCCCTAATCA GCCACCTGCC GCAGCTCACA CTGCCAACTT CCTCCTCAAC 
GCCAGCGGCA GCCCTTCGAC TCCAGCACCA AGCAGAACAG CTTCCTTCTC TGAGTCCAAA 
GCAGATGATA TTGCTCCAGC CAAGAAGGCA AAGCCAACAG CACCTCTTGA TTCAGTCCCA 
GCTTCAAGAC CTGGTCCAGG TAAAGCTACA ACCCTGTTCA GCCGTCACAC CAAAGCTATT 
ATTTGGGGGA TGCAGACACG GGCTGTGCAA GGAATGCTGG ACTTCGATTA TATTTGCTCC 
CGGGATGAAC CTTCAGTAGC TGCCATGGTT TATCCGTTCA CTGGTGATCA CAAGCAGAAG 
TTCTATTGGG GCCACAAAGA AATCCTGATC CCAGTCTACA AGAACATGTC TGATGCTATG 
AGGAAGCACC CTGAGGTGGA CGTTCTAATC AACTTTGCAT CTCTGCGGTC TGCTTATGAC 
AGCACTATTG AAACCATGAA CTATCCACAG ATCCGCACCA TTGCCATTAT TGCTGAGGGC 
ATTCCAGAGG CACTGACGCG CAAGCTGATC AAGACAGCTG ATAAGAAAGG AGTCACGATC 
ATTGGGCCTG CAACTGTTGG TGGGATCAAA CCAGGTTGCT TTAAAATAGG CAACACAGGA 
GGCATGCTGG ATAATATCTT AGCATCAAAA CTCTACCGTC CTGGCAGCGT GGCTTATGTC 
TCACGCTCTG GAGGAATGTC CAATGAGCTC AACAACATCA TTTCCCGAAC CACGGATGGG 
GTCTATGAAG GGGTGGCCAT TGGAGGAGAC AGATACCCTG GTTCAACTTT TATGGATCAT 
GTCTTACGCT ATGAGGATAC TCCAGGAGTG AAAATGATTG TAGTGCTTGG AGAGATTGGA 
GGCACAGAAG AGTATAAGAT CTCCAGGGGT ATTAAAGAAG GCCGTATTAC CAAGCCAGTA 
GTGTGCTGGT GTATTGGTAC CTGTGCCACC ATGTTCTCTT CAGAGGTACA GTTTGGCCAT 
GCAGGAGCTT GTGCCAACCA GGCTTCTGAA ACTGCTGTTG CAAAGAATCA GGCCTTGAAG 
GAAGCTGGGG TGTTTGTTCC CCGGAGTTTT GATGAGCTGG GAGAGGTCAT TCAGTCTGTC 
TATCAGGATC TTGTTGCCAA ACGAGTGATT GAACCAGCTG AGGAAGTGCC TCCTCCAACT 
GTGCCAATGG ATTATTCCTG GGCAAGGGAG CTGGGTCTGA TCCGCAAGCC AGCTTCCTTT 
ATGACCAGCA TCTGTGACGA GAGAGGTCAG GAACTGATTT ATGCTGGGAT GCCCATCACT 
GAGGTCTTCA AAGAAGAAAT GGGAATTGGA GGGGTTCTGG GACTGCTCTG GTTTCAAAGG 
AGGTTACCAA AGTATGCCTG TCAGTTTATC GAGATGTGTT TGATGGTAAC AGCAGATCAT 
GGGCCTGCTG TATCTGGAGC CCACAACACT ATTGTCTGCG CAAGAGCGGG AAAAGATCTT 
GTCTCAAGTC TCACTTCAGG CCTTCTTACT ATTGGTGACC GGTTTGGTGG GGCACTGGAT 
GCTGCAGCTA AAATGTTCAG CAAAGCCTTT GACAGTGGCA TTATCCCTAT GGAGTTTGTG 
AATAAAATGA AGAAGGAAGG GAAACTGATC ATGGGCATCG GACACAGGGT CAAATCGATA 
AATAATCCAG ACATGAGAGT TCAGATTCTT AAAGACTACG TGAAGCAGCA TTTCCCTGCC 
ACCCCACTGT TGGACTATGC ACTTGAAGTG GAAAAAATTA CAACTTCCAA GAAACCAAAT 
CTTATCCTGA ATGTAGATGG CTTTATTGGA GTTGCCTTTG TTGATGTACT CAGGAATTGT 
GGCTCTTTCA CACGGGAAGA AGCAGATGAA TATATTGATA TAGGAGCTCT TAATGGCATC 
TTCGTACTAG GCAGGAGTAT GGGATTCATT GGACACTACC TGGATCAGAA GAGGTTGAAG 
CAAGGTCTCT ACCGTCACCC ATGGGACGAC ATATCCTATG TTTTACCAGA GCACATGACT 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACLY ( XM_013946405.1 ) cDNA ORF clone, Apteryx australis mantelli -> XP_013801859.1 Apteryx australis mantelli ATP citrate lyase (ACLY), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAp16920
Clone ID Related Accession (Same CDS sequence)	XM_013946405.1
Accession Version	XM_013946405.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-08
Organism	Apteryx australis mantelli
Product	ATP-citrate synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013994976.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apteryx australis mantelli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGCCA AGGCCATCTC TGAACAGACT GGGAAGGAGT TCTTGTATAA GTATATCTGC 
ACATCCTCTG CTATCCAGAA CCGCTTCAAG TATGCCCGTG TCACCCCAGA CACTGACTGG 
GCGCGACTCA CCCAGGACCA CCCGTGGCTT CTGAGCGAGC GTCTAGTGGT GAAGCCAGAT 
CAGCTAATAA AACGGCGTGG GAAGCTTGGA CTGGTTGGAA TTAACTTCAC TCTAGATCAG 
GTGAAGGTTT GGCTGAAACA GCGTCTGGGC AAAGAAACCA CAATTGCTAA TGCTAAGGGA 
GTCCTGAAGA ATTTTCTGAT TGAGCCCTTC GTCCCCCACA GACAGGAAGA AGAGTTCTAT 
GTGTGCATAT ATGCTGCCCG CGAGGGAGAC TATGTACTCT TCCACCACGA AGGGGGTGTG 
GATGTGGGAG ATGTTGATGC CAAAGCTCAG AAGCTGCTCG TGGGGGTGGA TGAAAAGCTG 
AATGAATCTG ATGTAAAGAA ACATCTCCTG CAGCATGCAC CAGCAAATAA GAAAGATATC 
TTGGCTAGCT TCATCTCTGG CCTGTTCAAC CTTTATGAAG ATCTGTATTT CACGTACCTC 
GAGATCAATC CATTAGTTGT GACTCAAGAT GGTGTCTACA TCCTTGATTT GGCTGCGAAG 
ATTGATGCGA CTGCTGACTA CATCTGTAAA GTGAAATGGG GGGATGTGGA ATTCCCCCCT 
CCCTTTGGCA GGGAAGCCTA CCCAGAGGAA GCCTACATTG CTGACCTGGA TGCAAAGAGT 
GGAGCCAGCC TGAAACTTAC TATTCTCAAT CCCAAAGGCA GGATCTGGAC CATGGTGGCT 
GGTGGTGGTG CCTCTGTGGT TTACAGTGAC ACCATTTGTG ACCTGGGGGG TGTGAATGAA 
CTGGCTAACT ATGGTGAATA CTCAGGAGCC CCAAGTGAGC AACAAACTTA TGACTATGCT 
AAGACTATTC TCTCCCTCAT GACACGGGAG AAACACCCTG AAGGTAAAAT CCTAATCATT 
GGAGGCAGCA TCGCTAACTT CACCAATGTG GCAGCCACTT TTAAAGGCAT TGTGAGAGCA 
ATTAAAGACT ATCAAGGCCC TCTGAAGGAG CATGAAGTGA GGATCTTTGT GCGAAGGGGA 
GGCCCAAACT ACCAGGAAGG ACTGCGCGTC ATGGGAGAAG TGGGGAAGAC CACTGGGATC 
CCCATCCATG TCTTTGGCAC AGAGACTCAC ATGACTGCTA TTGTGGGCAT GGCACTTGGC 
CATCGGCCTA TCCCTAATCA GCCACCTGCC GCAGCTCACA CTGCCAACTT CCTCCTCAAC 
GCCAGCGGCA GCCCTTCGAC TCCAGCACCA AGCAGAACAG CTTCCTTCTC TGAGTCCAAA 
GCAGATGATA TTGCTCCAGC CAAGAAGGCA AAGCCAACAG CACCTCTTGG TAAAGCTACA 
ACCCTGTTCA GCCGTCACAC CAAAGCTATT ATTTGGGGGA TGCAGACACG GGCTGTGCAA 
GGAATGCTGG ACTTCGATTA TATTTGCTCC CGGGATGAAC CTTCAGTAGC TGCCATGGTT 
TATCCGTTCA CTGGTGATCA CAAGCAGAAG TTCTATTGGG GCCACAAAGA AATCCTGATC 
CCAGTCTACA AGAACATGTC TGATGCTATG AGGAAGCACC CTGAGGTGGA CGTTCTAATC 
AACTTTGCAT CTCTGCGGTC TGCTTATGAC AGCACTATTG AAACCATGAA CTATCCACAG 
ATCCGCACCA TTGCCATTAT TGCTGAGGGC ATTCCAGAGG CACTGACGCG CAAGCTGATC 
AAGACAGCTG ATAAGAAAGG AGTCACGATC ATTGGGCCTG CAACTGTTGG TGGGATCAAA 
CCAGGTTGCT TTAAAATAGG CAACACAGGA GGCATGCTGG ATAATATCTT AGCATCAAAA 
CTCTACCGTC CTGGCAGCGT GGCTTATGTC TCACGCTCTG GAGGAATGTC CAATGAGCTC 
AACAACATCA TTTCCCGAAC CACGGATGGG GTCTATGAAG GGGTGGCCAT TGGAGGAGAC 
AGATACCCTG GTTCAACTTT TATGGATCAT GTCTTACGCT ATGAGGATAC TCCAGGAGTG 
AAAATGATTG TAGTGCTTGG AGAGATTGGA GGCACAGAAG AGTATAAGAT CTCCAGGGGT 
ATTAAAGAAG GCCGTATTAC CAAGCCAGTA GTGTGCTGGT GTATTGGTAC CTGTGCCACC 
ATGTTCTCTT CAGAGGTACA GTTTGGCCAT GCAGGAGCTT GTGCCAACCA GGCTTCTGAA 
ACTGCTGTTG CAAAGAATCA GGCCTTGAAG GAAGCTGGGG TGTTTGTTCC CCGGAGTTTT 
GATGAGCTGG GAGAGGTCAT TCAGTCTGTC TATCAGGATC TTGTTGCCAA ACGAGTGATT 
GAACCAGCTG AGGAAGTGCC TCCTCCAACT GTGCCAATGG ATTATTCCTG GGCAAGGGAG 
CTGGGTCTGA TCCGCAAGCC AGCTTCCTTT ATGACCAGCA TCTGTGACGA GAGAGGTCAG 
GAACTGATTT ATGCTGGGAT GCCCATCACT GAGGTCTTCA AAGAAGAAAT GGGAATTGGA 
GGGGTTCTGG GACTGCTCTG GTTTCAAAGG AGGTTACCAA AGTATGCCTG TCAGTTTATC 
GAGATGTGTT TGATGGTAAC AGCAGATCAT GGGCCTGCTG TATCTGGAGC CCACAACACT 
ATTGTCTGCG CAAGAGCGGG AAAAGATCTT GTCTCAAGTC TCACTTCAGG CCTTCTTACT 
ATTGGTGACC GGTTTGGTGG GGCACTGGAT GCTGCAGCTA AAATGTTCAG CAAAGCCTTT 
GACAGTGGCA TTATCCCTAT GGAGTTTGTG AATAAAATGA AGAAGGAAGG GAAACTGATC 
ATGGGCATCG GACACAGGGT CAAATCGATA AATAATCCAG ACATGAGAGT TCAGATTCTT 
AAAGACTACG TGAAGCAGCA TTTCCCTGCC ACCCCACTGT TGGACTATGC ACTTGAAGTG 
GAAAAAATTA CAACTTCCAA GAAACCAAAT CTTATCCTGA ATGTAGATGG CTTTATTGGA 
GTTGCCTTTG TTGATGTACT CAGGAATTGT GGCTCTTTCA CACGGGAAGA AGCAGATGAA 
TATATTGATA TAGGAGCTCT TAATGGCATC TTCGTACTAG GCAGGAGTAT GGGATTCATT 
GGACACTACC TGGATCAGAA GAGGTTGAAG CAAGGTCTCT ACCGTCACCC ATGGGACGAC 
ATATCCTATG TTTTACCAGA GCACATGACT ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013801859.1
CDS	89..3364
Translation	MSAKAISEQTGKEFLYKYICTSSAIQNRFKYARVTPDTDWARLTQDHPWLLSERLVVKPDQLIKRRGKLGLVGINFTLDQVKVWLKQRLGKETTIANAKGVLKNFLIEPFVPHRQEEEFYVCIYAAREGDYVLFHHEGGVDVGDVDAKAQKLLVGVDEKLNESDVKKHLLQHAPANKKDILASFISGLFNLYEDLYFTYLEINPLVVTQDGVYILDLAAKIDATADYICKVKWGDVEFPPPFGREAYPEEAYIADLDAKSGASLKLTILNPKGRIWTMVAGGGASVVYSDTICDLGGVNELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPEGKILIIGGSIANFTNVAATFKGIVRAIKDYQGPLKEHEVRIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALGHRPIPNQPPAAAHTANFLLNASGSPSTPAPSRTASFSESKADDIAPAKKAKPTAPLGKATTLFSRHTKAIIWGMQTRAVQGMLDFDYICSRDEPSVAAMVYPFTGDHKQKFYWGHKEILIPVYKNMSDAMRKHPEVDVLINFASLRSAYDSTIETMNYPQIRTIAIIAEGIPEALTRKLIKTADKKGVTIIGPATVGGIKPGCFKIGNTGGMLDNILASKLYRPGSVAYVSRSGGMSNELNNIISRTTDGVYEGVAIGGDRYPGSTFMDHVLRYEDTPGVKMIVVLGEIGGTEEYKISRGIKEGRITKPVVCWCIGTCATMFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGEVIQSVYQDLVAKRVIEPAEEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITEVFKEEMGIGGVLGLLWFQRRLPKYACQFIEMCLMVTADHGPAVSGAHNTIVCARAGKDLVSSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGIIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDYVKQHFPATPLLDYALEVEKITTSKKPNLILNVDGFIGVAFVDVLRNCGSFTREEADEYIDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMTM

Target ORF information:

RefSeq Version	XM_013946405.1
Organism	Apteryx australis mantelli
Definition	Apteryx australis mantelli ATP citrate lyase (ACLY), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013946405.1

ORF Insert Sequence:

ATGTCAGCCA AGGCCATCTC TGAACAGACT GGGAAGGAGT TCTTGTATAA GTATATCTGC 
ACATCCTCTG CTATCCAGAA CCGCTTCAAG TATGCCCGTG TCACCCCAGA CACTGACTGG 
GCGCGACTCA CCCAGGACCA CCCGTGGCTT CTGAGCGAGC GTCTAGTGGT GAAGCCAGAT 
CAGCTAATAA AACGGCGTGG GAAGCTTGGA CTGGTTGGAA TTAACTTCAC TCTAGATCAG 
GTGAAGGTTT GGCTGAAACA GCGTCTGGGC AAAGAAACCA CAATTGCTAA TGCTAAGGGA 
GTCCTGAAGA ATTTTCTGAT TGAGCCCTTC GTCCCCCACA GACAGGAAGA AGAGTTCTAT 
GTGTGCATAT ATGCTGCCCG CGAGGGAGAC TATGTACTCT TCCACCACGA AGGGGGTGTG 
GATGTGGGAG ATGTTGATGC CAAAGCTCAG AAGCTGCTCG TGGGGGTGGA TGAAAAGCTG 
AATGAATCTG ATGTAAAGAA ACATCTCCTG CAGCATGCAC CAGCAAATAA GAAAGATATC 
TTGGCTAGCT TCATCTCTGG CCTGTTCAAC CTTTATGAAG ATCTGTATTT CACGTACCTC 
GAGATCAATC CATTAGTTGT GACTCAAGAT GGTGTCTACA TCCTTGATTT GGCTGCGAAG 
ATTGATGCGA CTGCTGACTA CATCTGTAAA GTGAAATGGG GGGATGTGGA ATTCCCCCCT 
CCCTTTGGCA GGGAAGCCTA CCCAGAGGAA GCCTACATTG CTGACCTGGA TGCAAAGAGT 
GGAGCCAGCC TGAAACTTAC TATTCTCAAT CCCAAAGGCA GGATCTGGAC CATGGTGGCT 
GGTGGTGGTG CCTCTGTGGT TTACAGTGAC ACCATTTGTG ACCTGGGGGG TGTGAATGAA 
CTGGCTAACT ATGGTGAATA CTCAGGAGCC CCAAGTGAGC AACAAACTTA TGACTATGCT 
AAGACTATTC TCTCCCTCAT GACACGGGAG AAACACCCTG AAGGTAAAAT CCTAATCATT 
GGAGGCAGCA TCGCTAACTT CACCAATGTG GCAGCCACTT TTAAAGGCAT TGTGAGAGCA 
ATTAAAGACT ATCAAGGCCC TCTGAAGGAG CATGAAGTGA GGATCTTTGT GCGAAGGGGA 
GGCCCAAACT ACCAGGAAGG ACTGCGCGTC ATGGGAGAAG TGGGGAAGAC CACTGGGATC 
CCCATCCATG TCTTTGGCAC AGAGACTCAC ATGACTGCTA TTGTGGGCAT GGCACTTGGC 
CATCGGCCTA TCCCTAATCA GCCACCTGCC GCAGCTCACA CTGCCAACTT CCTCCTCAAC 
GCCAGCGGCA GCCCTTCGAC TCCAGCACCA AGCAGAACAG CTTCCTTCTC TGAGTCCAAA 
GCAGATGATA TTGCTCCAGC CAAGAAGGCA AAGCCAACAG CACCTCTTGG TAAAGCTACA 
ACCCTGTTCA GCCGTCACAC CAAAGCTATT ATTTGGGGGA TGCAGACACG GGCTGTGCAA 
GGAATGCTGG ACTTCGATTA TATTTGCTCC CGGGATGAAC CTTCAGTAGC TGCCATGGTT 
TATCCGTTCA CTGGTGATCA CAAGCAGAAG TTCTATTGGG GCCACAAAGA AATCCTGATC 
CCAGTCTACA AGAACATGTC TGATGCTATG AGGAAGCACC CTGAGGTGGA CGTTCTAATC 
AACTTTGCAT CTCTGCGGTC TGCTTATGAC AGCACTATTG AAACCATGAA CTATCCACAG 
ATCCGCACCA TTGCCATTAT TGCTGAGGGC ATTCCAGAGG CACTGACGCG CAAGCTGATC 
AAGACAGCTG ATAAGAAAGG AGTCACGATC ATTGGGCCTG CAACTGTTGG TGGGATCAAA 
CCAGGTTGCT TTAAAATAGG CAACACAGGA GGCATGCTGG ATAATATCTT AGCATCAAAA 
CTCTACCGTC CTGGCAGCGT GGCTTATGTC TCACGCTCTG GAGGAATGTC CAATGAGCTC 
AACAACATCA TTTCCCGAAC CACGGATGGG GTCTATGAAG GGGTGGCCAT TGGAGGAGAC 
AGATACCCTG GTTCAACTTT TATGGATCAT GTCTTACGCT ATGAGGATAC TCCAGGAGTG 
AAAATGATTG TAGTGCTTGG AGAGATTGGA GGCACAGAAG AGTATAAGAT CTCCAGGGGT 
ATTAAAGAAG GCCGTATTAC CAAGCCAGTA GTGTGCTGGT GTATTGGTAC CTGTGCCACC 
ATGTTCTCTT CAGAGGTACA GTTTGGCCAT GCAGGAGCTT GTGCCAACCA GGCTTCTGAA 
ACTGCTGTTG CAAAGAATCA GGCCTTGAAG GAAGCTGGGG TGTTTGTTCC CCGGAGTTTT 
GATGAGCTGG GAGAGGTCAT TCAGTCTGTC TATCAGGATC TTGTTGCCAA ACGAGTGATT 
GAACCAGCTG AGGAAGTGCC TCCTCCAACT GTGCCAATGG ATTATTCCTG GGCAAGGGAG 
CTGGGTCTGA TCCGCAAGCC AGCTTCCTTT ATGACCAGCA TCTGTGACGA GAGAGGTCAG 
GAACTGATTT ATGCTGGGAT GCCCATCACT GAGGTCTTCA AAGAAGAAAT GGGAATTGGA 
GGGGTTCTGG GACTGCTCTG GTTTCAAAGG AGGTTACCAA AGTATGCCTG TCAGTTTATC 
GAGATGTGTT TGATGGTAAC AGCAGATCAT GGGCCTGCTG TATCTGGAGC CCACAACACT 
ATTGTCTGCG CAAGAGCGGG AAAAGATCTT GTCTCAAGTC TCACTTCAGG CCTTCTTACT 
ATTGGTGACC GGTTTGGTGG GGCACTGGAT GCTGCAGCTA AAATGTTCAG CAAAGCCTTT 
GACAGTGGCA TTATCCCTAT GGAGTTTGTG AATAAAATGA AGAAGGAAGG GAAACTGATC 
ATGGGCATCG GACACAGGGT CAAATCGATA AATAATCCAG ACATGAGAGT TCAGATTCTT 
AAAGACTACG TGAAGCAGCA TTTCCCTGCC ACCCCACTGT TGGACTATGC ACTTGAAGTG 
GAAAAAATTA CAACTTCCAA GAAACCAAAT CTTATCCTGA ATGTAGATGG CTTTATTGGA 
GTTGCCTTTG TTGATGTACT CAGGAATTGT GGCTCTTTCA CACGGGAAGA AGCAGATGAA 
TATATTGATA TAGGAGCTCT TAATGGCATC TTCGTACTAG GCAGGAGTAT GGGATTCATT 
GGACACTACC TGGATCAGAA GAGGTTGAAG CAAGGTCTCT ACCGTCACCC ATGGGACGAC 
ATATCCTATG TTTTACCAGA GCACATGACT ATGTGA