LIMCH1 cDNA ORF clone, Aptenodytes forsteri(emperor penguin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LIMCH1
Entrez Gene ID	103899629
Full Name	LIM and calponin homology domains 1
Gene Type	protein-coding
Organism	Aptenodytes forsteri(emperor penguin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019472302.1	XP_019327847.1	LIM and calponin homology domains-containing protein 1 isoform X4
XM_019472300.1	XP_019327845.1	LIM and calponin homology domains-containing protein 1 isoform X1
XM_019472303.1	XP_019327848.1	LIM and calponin homology domains-containing protein 1 isoform X4
XM_019472301.1	XP_019327846.1	LIM and calponin homology domains-containing protein 1 isoform X2
XM_009280131.2	XP_009278406.1	LIM and calponin homology domains-containing protein 1 isoform X3
XM_009280130.2	XP_009278405.1	LIM and calponin homology domains-containing protein 1 isoform X5
XM_019472304.1	XP_019327849.1	LIM and calponin homology domains-containing protein 1 isoform X6

Pathways from BioSystems
Gene Ontology

The following LIMCH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIMCH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAp53060	XM_009280131.2 Latest version!	Aptenodytes forsteri LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OAp53058	XM_019472302.1 Latest version!	Aptenodytes forsteri LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAp53058	XM_019472300.1 Latest version!	Aptenodytes forsteri LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAp53058	XM_019472303.1 Latest version!	Aptenodytes forsteri LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAp53059	XM_019472301.1 Latest version!	Aptenodytes forsteri LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAp53061	XM_009280130.2 Latest version!	Aptenodytes forsteri LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAp53062	XM_019472304.1 Latest version!	Aptenodytes forsteri LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LIMCH1 ( XM_009280131.2 ) cDNA ORF clone, Aptenodytes forsteri(emperor penguin) -> XP_009278406.1 Aptenodytes forsteri LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OAp53060
Clone ID Related Accession (Same CDS sequence)	XM_009280131.2
Accession Version	XM_009280131.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Aptenodytes forsteri(emperor penguin)
Product	LIM and calponin homology domains-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008795334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2016 this sequence version replaced XM_009280131.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aptenodytes forsteri Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAGG AAACGGAGGA TATTGAGAGC CCAAAGCGCA GCATTCGTGA CAGCGGCTAT 
ATAGACTGCT GGGACTCTGA ACGCAGCGAT TCCCTCTCCC CGCCTCGCCA CGGCAGAGAT 
GATTCCTTTG ACAGTCTGGA CTCCTTTGGC TCCCGCTCGC AGCAGACACC GTCTCCAGAT 
GTAGTGCTCA GAGGGAGCAG CGATGGGAGA GGAAGTGACT CGGAAACTGA CCTGCCACAC 
AGAAAGATGC CAGATGTGAA AAAAGATGAT ATGTTGGCGA GGCGGACCTC ACATGGTGAA 
CCTAAATCAG CTGTGCCTTT TAACCAGTAC CTCCCTAACA AGAGTAATCA GACTTTCTAT 
GTACCTGCTC CACTTCGGAA AAAGAAAGCT GAACGAGAAG AGTACCGAAA GAGCTGGAGC 
ACGGCGACTT CACCACTAGG AGACAGACCT TTCAGCAAAC CCCACCCTGA AACCATAGAA 
GAAGAACAGA ATGAAACATC AGGACTGTGT GAAAAGGAGA AAGTGGGCTC CCTGGCTCCT 
GAGGCAAGCT CCCTTAAAAA TCAAGTAGGT TTGAATCAAA ACGAAGGAAA CTCCATAGAC 
CAGTCTCCTT CATGTAGTGC AAAAGAAACA TCAGCACCCA AGGGGAAAGA TGCAGAAGAG 
ATCAAAAAGT TAAGAAGACT CGAACAAGCT GGCATTAAGG TCATGTCAGC AGCCCAGCGC 
TATGGCAGTA AAAAGCCAAC ATGTGAAGAC AACGGAAGCA CCCCAGACAT CATCCTTCGC 
AGGGACAACC CCTTCATGAC ACATTACCAA GGGAAGGATT CTGGTTCAGA AGATGAAGCA 
GCCAGCAGAG TTCCTGATTT AGAAAAGGAT GACTTTGCCA CCCGGAGAGC CAGAATGAAT 
CAACCCAAAC ACATAGTTCC GTTTAACCCC TTTTTAATTG GACCCTATAC AAGTAAAGAT 
AAAGGTAAAC TGGAAGACAT TAAAAAGCCA TTGCAGATGG AAAAGCAGGA ACATGCAAGA 
AGAAGACGGG TGCATATGGC ATCAACAAGG CAAATAGTGA CAAGCAAAGA GAACCCACTT 
CTGCAAACTG AGAAGACTGA GTCAGAGGAG GAACAGCAGG TGAAAGTGCC AGATACTGAG 
AGCGATGATT TGGCTAAACA GAAATCTCAA AGTACCCTTC CTCAGCTACG AGAAGATGCG 
ATTTTTGTGA ATGCCTCTAT AACAAAAGCA GATCTGGAGA CCTGGAAAAG ATTGAAACTG 
TCAGGGGAGC CCAGTGAAGA AGATTTCAAA TGTCCCAGTG ACACTCTGCC ACCAGCTGAA 
ATCACGGTGA AAGCAGCGGC CCACGATGAT TTTGCTGTCC GCAAAGCCAG AGCAAACAAA 
AAGGCTGGTA GCCCCAAGCA GAAGTTTGTG CATTTTGGGC CAGTAACTGA GATTGATCAA 
CAGAAATGGG ACAAGCTCGT TATTGGCAGA GCTTCATCCA AAAATGGAAC AGAAGAGGAA 
AGCAAGAAAG ACAGTGTGGA GAATCACTCT GCTGTTGAAA GCTCTCTTTC AACCACTGTT 
GGCAGTCTCG GGTTCGCTGA ACACAGCGAT GAAGTATCTA AGCCACAGAA TGAATCCAGT 
ACTGTCACAC GAGAAGAGAG CCAAGAACAG CCTGAAATCC CTCAGCAGCA GAGCGGTGGA 
ACAGAAAACG AAGTGAAATT TCCAGACCCG CAAAGAGATG ACATGTTGGC CAGAAGAACT 
GGAGCTTTCC TAAAACAGCC TGGGCCCAGT GTTAAGCAGT TCCTTCCCAT GCCATTTTCA 
AAGCAGCAGA ATAAGCAGGG CACAGCTAGA GATCTTGAGA AGAAAAATAC GAGGCCTGAA 
AAGTCTCCAC AAGTCTGCAT TAATAGCTCT GGAAAACCTG TAGTACAACC GCCGCAAGAA 
CAGCAGCAAG GGCAGGAAAA ATGCAATCTC CCTCCTGCAG GACAAGATGA GACTATTTGT 
ACTGCCCAAG TGGGTGAAGG TAGCCTTAAT GAAGTCCACC TTGGTCAAAT GTCTAAGCTT 
CAGGAGGTTC CTGAGGAAAA CAAAACGGAA ACATTGTCAT CCAAGGATGA GCATCTGCAA 
ACATTCGGCC CCAGAACTGC TGTGTCTGAT GACGCAGAGA GTATGAGCAT GTTTGACATG 
AGATGTGAAG AAGAAGCTGT GACTCAGCCT CATAGCAAAG CTCGCCATGA GAAACTGCAG 
AACGTACACA ACCAGCTGAA AGAGGATGAG ACCAGATGGC AAGATGACCT GGCTCGGTGG 
AAGAGTCGTC GAAGGAGCGC TTCACAGGAT TTAATAAAAA AAGAAGCTGA AAGGAAGAAA 
ATGGAAAGGT TATTGTCTGG AGATGGAACG AATGAGCGCA GAAAAAGCAT CAAAACCTAC 
CGAGAAATTG TGGAAGAGAA AGAGAGAAGA GAGCGGGAGC TTCATGAGGC ATACAAGAAT 
GCAAAGTCAC AAGAGGAGGC TGAAAGCATC CTCCAGCAGT ACATCGAAAG ATTCACTATC 
AGTGAAGCTG TCCTTGAGCG TTTGCAGATG CCAAAAATTC TGGAAAGAAG CCATTCAGTG 
GAGCCAAATT CCCCACTGAA AGACCCAAAT CCTCTGAGAT ATTTAAGGCA ACAGTCACTG 
CCTGCTCCAA AATTCACTGC CACCGTTGAA GCAACTATTG TTCCCACCAC TGAAATAGAG 
CCCAGTGGTT CGGCGGGCCG CACATCTCCC AGCAAAAGCA TTGTCTCCAA GGCTGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCACAACCC AAAAATACAC AGGAAGTTTT AAAGAACTTT 
AAGGTAGATG GAAAAGTCAG CATGAATGGA GAAAATTTCA ACGGTGTGGA AGAGAAGGAT 
AAAGAGTGTA CAACAGTAAT ACTTACTCCT TCGCCAAGCA GATCTCTGAA ATTTGACGGA 
GTAGCCAGAG GGGACAGGCC CCCGGTAGAG TTGAAGAAGG ACCCCACAAG TGTTGAGCTC 
AGTTTACAGA GGCCTGCCAC TCAGAGTGTG CACAAAGAGC CAACAGCCTC ACTGGTGGAA 
GCAGATAGAG CTGCCAGCAA ACAGGTCGAG GCTAGCCCTG GAAGTGCAGG ACCTGTGAGC 
CCGGTGTGCC CGTGCCCAGG TTCAGATCAA AAGCAATTCA AGTCAACTGC AGCTTGTGCC 
AGCCCTGTTG TGAAGAGATT AGAATTTCTT CCCAGTCCGT TCCCTTCAGA GGAAGCTGCT 
TCAAGTGAGA AAGATGTGAA GAAACCTGAA ATGGAGCAGC AGAAGGAAGC AGAGCTTCCT 
GCTGCACCGG AGGTGATGAA ACCAAAAGCC CTGGAACAAG AGGGGAGTGT AGTATTGCCA 
TTTTTAAAGA AACTACCCGA GACCAGCCAA GTTACTCTGC AGAATTCTGG TCTACAAGCT 
GATTCTGATA GTGGTGACAA ATCAAATCGT TTCAATTTCT GGTCCTGGGA TCCAGAAGAA 
GAGCGAAAGC GTCAGGAAAG GTGGCAGCAT GAGCAAGAAC GCTTGCTTCA GGAGAAGTAT 
CAGAAGGAGC AGGACAAGCT GAAAGAGGAA TGGGAAAAGG CCCAGAGAGA AGTTGAAGAG 
GAGGAGCGTA GATACTATGA GGAGGAACGT AAGATAATTG AGGATACTGT AGTTCCATTC 
ATTGTTTCTT CAAACTCTGC TGAGCTCCTC TCCTCCTCTT CCTCTACCAC TGAAGGAAAC 
AAGACAGCGA ACACAACTGA TTCAAACAGC TCTCCAGAGG AAGTCAAACA AAAAGAAGTT 
ACAAGGCAGA AAAAGTTGCT CTGGGAGCAG GACAGTATAC CATCTCCGAA AAGCAAGGAA 
AATGAACTCT GGCAAGAAAA GAGGAGTGGA AATAAAGTGC TCTCAGGACA CCCGGAGATG 
GGTATCTTGA AAGGAGGTGA ACAGGAGCAC CAGGTGCCAG AGATGGAGCA CAGCTCGCCT 
GCCAGGCTGA ATCTGCCGTT GACTCAGGAC GTCTCCTGGA ATCAGCAGTT GCTGACAAAG 
CAGTCCCCAC ATCGTGCAGA GGACATTCGG CAGAGACCAT TCCTGAGCCA GAGTGCGGGA 
CAACAGCCGA GCTCCGCAGG AGAAACGAGG AGGGCAGGCT ATCATGAGAA CTTCCAATCT 
GGGCCATCAT CTCCCTGCTC TCCTGCTGCT CCGAGTCAGT CACCCAACAG GTACAAAAGG 
TCTGTCAGTG GAAAGAAGCT TTGTTCTACC TGTGGGCTTC CTCTTGGGAA AGGAGCTGCC 
ATGATTATTG AAACGCTTGG TCTTTATTTC CATATTCAGT GCTTCAGGTG TGGCATTTGC 
AAGGGACAGC TGGGAGACGC AGCAAGCGGG ACAGATGTCA GGATTAGAAA TGGTCTTCTT 
AACTGCACCG ATTGTTATAT CCGATCCAGA ACTGCTGGAC AGCCAACTAC ATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009278406.1
CDS	241..4677
Translation	MRKETEDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSETDLPHRKMPDVKKDDMLARRTSHGEPKSAVPFNQYLPNKSNQTFYVPAPLRKKKAEREEYRKSWSTATSPLGDRPFSKPHPETIEEEQNETSGLCEKEKVGSLAPEASSLKNQVGLNQNEGNSIDQSPSCSAKETSAPKGKDAEEIKKLRRLEQAGIKVMSAAQRYGSKKPTCEDNGSTPDIILRRDNPFMTHYQGKDSGSEDEAASRVPDLEKDDFATRRARMNQPKHIVPFNPFLIGPYTSKDKGKLEDIKKPLQMEKQEHARRRRVHMASTRQIVTSKENPLLQTEKTESEEEQQVKVPDTESDDLAKQKSQSTLPQLREDAIFVNASITKADLETWKRLKLSGEPSEEDFKCPSDTLPPAEITVKAAAHDDFAVRKARANKKAGSPKQKFVHFGPVTEIDQQKWDKLVIGRASSKNGTEEESKKDSVENHSAVESSLSTTVGSLGFAEHSDEVSKPQNESSTVTREESQEQPEIPQQQSGGTENEVKFPDPQRDDMLARRTGAFLKQPGPSVKQFLPMPFSKQQNKQGTARDLEKKNTRPEKSPQVCINSSGKPVVQPPQEQQQGQEKCNLPPAGQDETICTAQVGEGSLNEVHLGQMSKLQEVPEENKTETLSSKDEHLQTFGPRTAVSDDAESMSMFDMRCEEEAVTQPHSKARHEKLQNVHNQLKEDETRWQDDLARWKSRRRSASQDLIKKEAERKKMERLLSGDGTNERRKSIKTYREIVEEKERRERELHEAYKNAKSQEEAESILQQYIERFTISEAVLERLQMPKILERSHSVEPNSPLKDPNPLRYLRQQSLPAPKFTATVEATIVPTTEIEPSGSAGRTSPSKSIVSKAVPMLTPKPYSQPKNTQEVLKNFKVDGKVSMNGENFNGVEEKDKECTTVILTPSPSRSLKFDGVARGDRPPVELKKDPTSVELSLQRPATQSVHKEPTASLVEADRAASKQVEASPGSAGPVSPVCPCPGSDQKQFKSTAACASPVVKRLEFLPSPFPSEEAASSEKDVKKPEMEQQKEAELPAAPEVMKPKALEQEGSVVLPFLKKLPETSQVTLQNSGLQADSDSGDKSNRFNFWSWDPEEERKRQERWQHEQERLLQEKYQKEQDKLKEEWEKAQREVEEEERRYYEEERKIIEDTVVPFIVSSNSAELLSSSSSTTEGNKTANTTDSNSSPEEVKQKEVTRQKKLLWEQDSIPSPKSKENELWQEKRSGNKVLSGHPEMGILKGGEQEHQVPEMEHSSPARLNLPLTQDVSWNQQLLTKQSPHRAEDIRQRPFLSQSAGQQPSSAGETRRAGYHENFQSGPSSPCSPAAPSQSPNRYKRSVSGKKLCSTCGLPLGKGAAMIIETLGLYFHIQCFRCGICKGQLGDAASGTDVRIRNGLLNCTDCYIRSRTAGQPTTL

Target ORF information:

RefSeq Version	XM_009280131.2
Organism	Aptenodytes forsteri(emperor penguin)
Definition	Aptenodytes forsteri LIM and calponin homology domains 1 (LIMCH1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009280131.2

ORF Insert Sequence:

ATGAGAAAGG AAACGGAGGA TATTGAGAGC CCAAAGCGCA GCATTCGTGA CAGCGGCTAT 
ATAGACTGCT GGGACTCTGA ACGCAGCGAT TCCCTCTCCC CGCCTCGCCA CGGCAGAGAT 
GATTCCTTTG ACAGTCTGGA CTCCTTTGGC TCCCGCTCGC AGCAGACACC GTCTCCAGAT 
GTAGTGCTCA GAGGGAGCAG CGATGGGAGA GGAAGTGACT CGGAAACTGA CCTGCCACAC 
AGAAAGATGC CAGATGTGAA AAAAGATGAT ATGTTGGCGA GGCGGACCTC ACATGGTGAA 
CCTAAATCAG CTGTGCCTTT TAACCAGTAC CTCCCTAACA AGAGTAATCA GACTTTCTAT 
GTACCTGCTC CACTTCGGAA AAAGAAAGCT GAACGAGAAG AGTACCGAAA GAGCTGGAGC 
ACGGCGACTT CACCACTAGG AGACAGACCT TTCAGCAAAC CCCACCCTGA AACCATAGAA 
GAAGAACAGA ATGAAACATC AGGACTGTGT GAAAAGGAGA AAGTGGGCTC CCTGGCTCCT 
GAGGCAAGCT CCCTTAAAAA TCAAGTAGGT TTGAATCAAA ACGAAGGAAA CTCCATAGAC 
CAGTCTCCTT CATGTAGTGC AAAAGAAACA TCAGCACCCA AGGGGAAAGA TGCAGAAGAG 
ATCAAAAAGT TAAGAAGACT CGAACAAGCT GGCATTAAGG TCATGTCAGC AGCCCAGCGC 
TATGGCAGTA AAAAGCCAAC ATGTGAAGAC AACGGAAGCA CCCCAGACAT CATCCTTCGC 
AGGGACAACC CCTTCATGAC ACATTACCAA GGGAAGGATT CTGGTTCAGA AGATGAAGCA 
GCCAGCAGAG TTCCTGATTT AGAAAAGGAT GACTTTGCCA CCCGGAGAGC CAGAATGAAT 
CAACCCAAAC ACATAGTTCC GTTTAACCCC TTTTTAATTG GACCCTATAC AAGTAAAGAT 
AAAGGTAAAC TGGAAGACAT TAAAAAGCCA TTGCAGATGG AAAAGCAGGA ACATGCAAGA 
AGAAGACGGG TGCATATGGC ATCAACAAGG CAAATAGTGA CAAGCAAAGA GAACCCACTT 
CTGCAAACTG AGAAGACTGA GTCAGAGGAG GAACAGCAGG TGAAAGTGCC AGATACTGAG 
AGCGATGATT TGGCTAAACA GAAATCTCAA AGTACCCTTC CTCAGCTACG AGAAGATGCG 
ATTTTTGTGA ATGCCTCTAT AACAAAAGCA GATCTGGAGA CCTGGAAAAG ATTGAAACTG 
TCAGGGGAGC CCAGTGAAGA AGATTTCAAA TGTCCCAGTG ACACTCTGCC ACCAGCTGAA 
ATCACGGTGA AAGCAGCGGC CCACGATGAT TTTGCTGTCC GCAAAGCCAG AGCAAACAAA 
AAGGCTGGTA GCCCCAAGCA GAAGTTTGTG CATTTTGGGC CAGTAACTGA GATTGATCAA 
CAGAAATGGG ACAAGCTCGT TATTGGCAGA GCTTCATCCA AAAATGGAAC AGAAGAGGAA 
AGCAAGAAAG ACAGTGTGGA GAATCACTCT GCTGTTGAAA GCTCTCTTTC AACCACTGTT 
GGCAGTCTCG GGTTCGCTGA ACACAGCGAT GAAGTATCTA AGCCACAGAA TGAATCCAGT 
ACTGTCACAC GAGAAGAGAG CCAAGAACAG CCTGAAATCC CTCAGCAGCA GAGCGGTGGA 
ACAGAAAACG AAGTGAAATT TCCAGACCCG CAAAGAGATG ACATGTTGGC CAGAAGAACT 
GGAGCTTTCC TAAAACAGCC TGGGCCCAGT GTTAAGCAGT TCCTTCCCAT GCCATTTTCA 
AAGCAGCAGA ATAAGCAGGG CACAGCTAGA GATCTTGAGA AGAAAAATAC GAGGCCTGAA 
AAGTCTCCAC AAGTCTGCAT TAATAGCTCT GGAAAACCTG TAGTACAACC GCCGCAAGAA 
CAGCAGCAAG GGCAGGAAAA ATGCAATCTC CCTCCTGCAG GACAAGATGA GACTATTTGT 
ACTGCCCAAG TGGGTGAAGG TAGCCTTAAT GAAGTCCACC TTGGTCAAAT GTCTAAGCTT 
CAGGAGGTTC CTGAGGAAAA CAAAACGGAA ACATTGTCAT CCAAGGATGA GCATCTGCAA 
ACATTCGGCC CCAGAACTGC TGTGTCTGAT GACGCAGAGA GTATGAGCAT GTTTGACATG 
AGATGTGAAG AAGAAGCTGT GACTCAGCCT CATAGCAAAG CTCGCCATGA GAAACTGCAG 
AACGTACACA ACCAGCTGAA AGAGGATGAG ACCAGATGGC AAGATGACCT GGCTCGGTGG 
AAGAGTCGTC GAAGGAGCGC TTCACAGGAT TTAATAAAAA AAGAAGCTGA AAGGAAGAAA 
ATGGAAAGGT TATTGTCTGG AGATGGAACG AATGAGCGCA GAAAAAGCAT CAAAACCTAC 
CGAGAAATTG TGGAAGAGAA AGAGAGAAGA GAGCGGGAGC TTCATGAGGC ATACAAGAAT 
GCAAAGTCAC AAGAGGAGGC TGAAAGCATC CTCCAGCAGT ACATCGAAAG ATTCACTATC 
AGTGAAGCTG TCCTTGAGCG TTTGCAGATG CCAAAAATTC TGGAAAGAAG CCATTCAGTG 
GAGCCAAATT CCCCACTGAA AGACCCAAAT CCTCTGAGAT ATTTAAGGCA ACAGTCACTG 
CCTGCTCCAA AATTCACTGC CACCGTTGAA GCAACTATTG TTCCCACCAC TGAAATAGAG 
CCCAGTGGTT CGGCGGGCCG CACATCTCCC AGCAAAAGCA TTGTCTCCAA GGCTGTGCCT 
ATGCTGACAC CCAAGCCTTA CTCACAACCC AAAAATACAC AGGAAGTTTT AAAGAACTTT 
AAGGTAGATG GAAAAGTCAG CATGAATGGA GAAAATTTCA ACGGTGTGGA AGAGAAGGAT 
AAAGAGTGTA CAACAGTAAT ACTTACTCCT TCGCCAAGCA GATCTCTGAA ATTTGACGGA 
GTAGCCAGAG GGGACAGGCC CCCGGTAGAG TTGAAGAAGG ACCCCACAAG TGTTGAGCTC 
AGTTTACAGA GGCCTGCCAC TCAGAGTGTG CACAAAGAGC CAACAGCCTC ACTGGTGGAA 
GCAGATAGAG CTGCCAGCAA ACAGGTCGAG GCTAGCCCTG GAAGTGCAGG ACCTGTGAGC 
CCGGTGTGCC CGTGCCCAGG TTCAGATCAA AAGCAATTCA AGTCAACTGC AGCTTGTGCC 
AGCCCTGTTG TGAAGAGATT AGAATTTCTT CCCAGTCCGT TCCCTTCAGA GGAAGCTGCT 
TCAAGTGAGA AAGATGTGAA GAAACCTGAA ATGGAGCAGC AGAAGGAAGC AGAGCTTCCT 
GCTGCACCGG AGGTGATGAA ACCAAAAGCC CTGGAACAAG AGGGGAGTGT AGTATTGCCA 
TTTTTAAAGA AACTACCCGA GACCAGCCAA GTTACTCTGC AGAATTCTGG TCTACAAGCT 
GATTCTGATA GTGGTGACAA ATCAAATCGT TTCAATTTCT GGTCCTGGGA TCCAGAAGAA 
GAGCGAAAGC GTCAGGAAAG GTGGCAGCAT GAGCAAGAAC GCTTGCTTCA GGAGAAGTAT 
CAGAAGGAGC AGGACAAGCT GAAAGAGGAA TGGGAAAAGG CCCAGAGAGA AGTTGAAGAG 
GAGGAGCGTA GATACTATGA GGAGGAACGT AAGATAATTG AGGATACTGT AGTTCCATTC 
ATTGTTTCTT CAAACTCTGC TGAGCTCCTC TCCTCCTCTT CCTCTACCAC TGAAGGAAAC 
AAGACAGCGA ACACAACTGA TTCAAACAGC TCTCCAGAGG AAGTCAAACA AAAAGAAGTT 
ACAAGGCAGA AAAAGTTGCT CTGGGAGCAG GACAGTATAC CATCTCCGAA AAGCAAGGAA 
AATGAACTCT GGCAAGAAAA GAGGAGTGGA AATAAAGTGC TCTCAGGACA CCCGGAGATG 
GGTATCTTGA AAGGAGGTGA ACAGGAGCAC CAGGTGCCAG AGATGGAGCA CAGCTCGCCT 
GCCAGGCTGA ATCTGCCGTT GACTCAGGAC GTCTCCTGGA ATCAGCAGTT GCTGACAAAG 
CAGTCCCCAC ATCGTGCAGA GGACATTCGG CAGAGACCAT TCCTGAGCCA GAGTGCGGGA 
CAACAGCCGA GCTCCGCAGG AGAAACGAGG AGGGCAGGCT ATCATGAGAA CTTCCAATCT 
GGGCCATCAT CTCCCTGCTC TCCTGCTGCT CCGAGTCAGT CACCCAACAG GTACAAAAGG 
TCTGTCAGTG GAAAGAAGCT TTGTTCTACC TGTGGGCTTC CTCTTGGGAA AGGAGCTGCC 
ATGATTATTG AAACGCTTGG TCTTTATTTC CATATTCAGT GCTTCAGGTG TGGCATTTGC 
AAGGGACAGC TGGGAGACGC AGCAAGCGGG ACAGATGTCA GGATTAGAAA TGGTCTTCTT 
AACTGCACCG ATTGTTATAT CCGATCCAGA ACTGCTGGAC AGCCAACTAC ATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_019472302.1 ) cDNA ORF clone, Aptenodytes forsteri(emperor penguin) -> XP_019327847.1 Aptenodytes forsteri LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OAp53058
Clone ID Related Accession (Same CDS sequence)	XM_019472302.1 , XM_019472300.1 , XM_019472303.1
Accession Version	XM_019472302.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Aptenodytes forsteri(emperor penguin)
Product	LIM and calponin homology domains-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008795334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aptenodytes forsteri Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAGG AAACGGAGGA TATTGAGAGC CCAAAGCGCA GCATTCGTGA CAGCGGCTAT 
ATAGACTGCT GGGACTCTGA ACGCAGCGAT TCCCTCTCCC CGCCTCGCCA CGGCAGAGAT 
GATTCCTTTG ACAGTCTGGA CTCCTTTGGC TCCCGCTCGC AGCAGACACC GTCTCCAGAT 
GTAGTGCTCA GAGGGAGCAG CGATGGGAGA GGAAGTGACT CGGAAACTGA CCTGCCACAC 
AGAAAGATGC CAGATGTGAA AAAAGATGAT ATGTTGGCGA GGCGGACCTC ACATGGTGAA 
CCTAAATCAG CTGTGCCTTT TAACCAGTAC CTCCCTAACA AGAGTAATCA GACTTTCTAT 
GTACCTGCTC CACTTCGGAA AAAGAAAGCT GAACGAGAAG AGTACCGAAA GAGCTGGAGC 
ACGGCGACTT CACCACTAGG AGACAGACCT TTCAGCAAAC CCCACCCTGA AACCATAGAA 
GAAGAACAGA ATGAAACATC AGGACTGTGT GAAAAGGAGA AAGTGGGCTC CCTGGCTCCT 
GAGGCAAGCT CCCTTAAAAA TCAAGTAGGT TTGAATCAAA ACGAAGGAAA CTCCATAGAC 
CAGTCTCCTT CATGTAGTGC AAAAGAAACA TCAGCACCCA AGGGGAAAGA TGCAGAAGAG 
ATCAAAAAGT TAAGAAGACT CGAACAAGCT GGCATTAAGG TCATGTCAGC AGCCCAGCGC 
TATGGCAGTA AAAAGCCAAC ATGTGAAGAC AACGGAAGCA CCCCAGACAT CATCCTTCGC 
AGGGACAACC CCTTCATGAC ACATTACCAA GGGAAGGATT CTGGTTCAGA AGATGAAGCA 
GCCAGCAGAG TTCCTGATTT AGAAAAGGAT GACTTTGCCA CCCGGAGAGC CAGAATGAAT 
CAACCCAAAC ACATAGTTCC GTTTAACCCC TTTTTAATTG GACCCTATAC AAGTAAAGAT 
AAAGGTAAAC TGGAAGACAT TAAAAAGCCA TTGCAGATGG AAAAGCAGGA ACATGCAAGA 
AGAAGACGGG TGCATATGGC ATCAACAAGG CAAATAGTGA CAAGCAAAGA GAACCCACTT 
CTGCAAACTG AGAAGACTGA GTCAGAGGAG GAACAGCAGG TGAAAGTGCC AGATACTGAG 
AGCGATGATT TGGCTAAACA GAAATCTCAA AGTACCCTTC CTCAGCTACG AGAAGATGCG 
ATTTTTGTGA ATGCCTCTAT AACAAAAGCA GATCTGGAGA CCTGGAAAAG ATTGAAACTG 
TCAGGGGAGC CCAGTGAAGA AGATTTCAAA TGTCCCAGTG ACACTCTGCC ACCAGCTGAA 
ATCACGGTGA AAGCAGCGGC CCACGATGAT TTTGCTGTCC GCAAAGCCAG AGCAAACAAA 
AAGGCTGGTA GCCCCAAGCA GAAGTTTGTG CATTTTGGGC CAGTAACTGA GATTGATCAA 
CAGAAATGGG ACAAGCTCGT TATTGGCAGA GCTTCATCCA AAAATGGAAC AGAAGAGGAA 
AGCAAGAAAG ACAGTGTGGA GAATCACTCT GCTGTTGAAA GCTCTCTTTC AACCACTGTT 
GGCAGTCTCG GGTTCGCTGA ACACAGCGAT GAAGTATCTA AGCCACAGAA TGAATCCAGT 
CGCATCGTTA GTGAGGTAGC CACGTCAGGT ACAGATGACT CTTGCAGGAG AATGCTATCA 
CCTGTCCCGG CTTTTGGCAT CGTCTCTGAG AAACAAACCC TGTGCAGTTT GAACAAACGG 
CAAGAAGGCA CCGAGGAAGA CTGCGTCGGG CATTTACCAG ACATTGAGAG AGATGATATG 
TTGGTTAGAA GGATGGGAAC TTTTCAGAGG CGTACCACTA GCCCTGTTCC CATACATTGT 
CCTCCCCTGT CAGTAGCTCC TCATCTGCTG CAGCAGCGGC AAGGGGAGGC AGTAGCTTGT 
GAGAAGGATT TGAAGACTCC ATGGGAAGCA GAAAGTACTG TCACACGAGA AGAGAGCCAA 
GAACAGCCTG AAATCCCTCA GCAGCAGAGC GGTGGAACAG AAAACGAAGT GAAATTTCCA 
GACCCGCAAA GAGATGACAT GTTGGCCAGA AGAACTGGAG CTTTCCTAAA ACAGCCTGGG 
CCCAGTGTTA AGCAGTTCCT TCCCATGCCA TTTTCAAAGC AGCAGAATAA GCAGGGCACA 
GCTAGAGATC TTGAGAAGAA AAATACGAGG CCTGAAAAGT CTCCACAAGT CTGCATTAAT 
AGCTCTGGAA AACCTGTAGT ACAACCGCCG CAAGAACAGC AGCAAGGGCA GGAAAAATGC 
AATCTCCCTC CTGCAGGACA AGATGAGACT ATTTGTACTG CCCAAGTGGG TGAAGGTAGC 
CTTAATGAAG TCCACCTTGG TCAAATGTCT AAGCTTCAGG AGGTTCCTGA GGAAAACAAA 
ACGGAAACAT TGTCATCCAA GGATGAGCAT CTGCAAACAT TCGGCCCCAG AACTGCTGTG 
TCTGATGACG CAGAGAGTAT GAGCATGTTT GACATGAGAT GTGAAGAAGA AGCTGTGACT 
CAGCCTCATA GCAAAGCTCG CCATGAGAAA CTGCAGAACG TACACAACCA GCTGAAAGAG 
GATGAGACCA GATGGCAAGA TGACCTGGCT CGGTGGAAGA GTCGTCGAAG GAGCGCTTCA 
CAGGATTTAA TAAAAAAAGA AGCTGAAAGG AAGAAAATGG AAAGGTTATT GTCTGGAGAT 
GGAACGAATG AGCGCAGAAA AAGCATCAAA ACCTACCGAG AAATTGTGGA AGAGAAAGAG 
AGAAGAGAGC GGGAGCTTCA TGAGGCATAC AAGAATGCAA AGTCACAAGA GGAGGCTGAA 
AGCATCCTCC AGCAGTACAT CGAAAGATTC ACTATCAGTG AAGCTGTCCT TGAGCGTTTG 
CAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAGTGGAGC CAAATTCCCC ACTGAAAGAC 
CCAAATCCTC TGAGATATTT AAGGCAACAG TCACTGCCTG CTCCAAAATT CACTGCCACC 
GTTGAAGCAA CTATTGTTCC CACCACTGAA ATAGAGCCCA GTGGTTCGGC GGGCCGCACA 
TCTCCCAGCA AAAGCATTGT CTCCAAGGCT GTGCCTATGC TGACACCCAA GCCTTACTCA 
CAACCCAAAA ATACACAGGA AGTTTTAAAG AACTTTAAGG TAGATGGAAA AGTCAGCATG 
AATGGAGAAA ATTTCAACGG TGTGGAAGAG AAGGATAAAG AGTGTACAAC AGTAATACTT 
ACTCCTTCGC CAAGCAGATC TCTGAAATTT GACGGAGTAG CCAGAGGGGA CAGGCCCCCG 
GTAGAGTTGA AGAAGGACCC CACAAGTGTT GAGCTCAGTT TACAGAGGCC TGCCACTCAG 
AGTGTGCACA AAGAGCCAAC AGCCTCACTG GTGGAAGCAG ATAGAGCTGC CAGCAAACAG 
GTCGAGGCTA GCCCTGGAAG TGCAGGACCT GTGAGCCCGG TGTGCCCGTG CCCAGGTTCA 
GATCAAAAGC AATTCAAGTC AACTGCAGCT TGTGCCAGCC CTGTTGTGAA GAGATTAGAA 
TTTCTTCCCA GTCCGTTCCC TTCAGAGGAA GCTGCTTCAA GTGAGAAAGA TGTGAAGAAA 
CCTGAAATGG AGCAGCAGAA GGAAGCAGAG CTTCCTGCTG CACCGGAGGT GATGAAACCA 
AAAGCCCTGG AACAAGAGGG GAGTGTAGTA TTGCCATTTT TAAAGAAACT ACCCGAGACC 
AGCCAAGTTA CTCTGCAGAA TTCTGGTCTA CAAGCTGATT CTGATAGTGG TGACAAATCA 
AATCGTTTCA ATTTCTGGTC CTGGGATCCA GAAGAAGAGC GAAAGCGTCA GGAAAGGTGG 
CAGCATGAGC AAGAACGCTT GCTTCAGGAG AAGTATCAGA AGGAGCAGGA CAAGCTGAAA 
GAGGAATGGG AAAAGGCCCA GAGAGAAGTT GAAGAGGAGG AGCGTAGATA CTATGAGGAG 
GAACGTAAGA TAATTGAGGA TACTGTAGTT CCATTCATTG TTTCTTCAAA CTCTGCTGAG 
CTCCTCTCCT CCTCTTCCTC TACCACTGAA GGAAACAAGA CAGCGAACAC AACTGATTCA 
AACAGCTCTC CAGAGGAAGT CAAACAAAAA GAAGTTACAA GGCAGAAAAA GTTGCTCTGG 
GAGCAGGACA GTATACCATC TCCGAAAAGC AAGGAAAATG AACTCTGGCA AGAAAAGAGG 
AGTGGAAATA AAGTGCTCTC AGGACACCCG GAGATGGGTA TCTTGAAAGG AGGTGAACAG 
GAGCACCAGG TGCCAGAGAT GGAGCACAGC TCGCCTGCCA GGCTGAATCT GCCGTTGACT 
CAGGACGTCT CCTGGAATCA GCAGTTGCTG ACAAAGCAGT CCCCACATCG TGCAGAGGAC 
ATTCGGCAGA GACCATTCCT GAGCCAGAGT GCGGGACAAC AGCCGAGCTC CGCAGGAGAA 
ACGAGGAGGG CAGGCTATCA TGAGAACTTC CAATCTGGGC CATCATCTCC CTGCTCTCCT 
GCTGCTCCGA GTCAGTCACC CAACAGGTAC AAAAGGTCTG TCAGTGGAAA GAAGCTTTGT 
TCTACCTGTG GGCTTCCTCT TGGGAAAGGA GCTGCCATGA TTATTGAAAC GCTTGGTCTT 
TATTTCCATA TTCAGTGCTT CAGGTGTGGC ATTTGCAAGG GACAGCTGGG AGACGCAGCA 
AGCGGGACAG ATGTCAGGAT TAGAAATGGT CTTCTTAACT GCACCGATTG TTATATCCGA 
TCCAGAACTG CTGGACAGCC AACTACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019327847.1
CDS	382..5154
Translation	MRKETEDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSETDLPHRKMPDVKKDDMLARRTSHGEPKSAVPFNQYLPNKSNQTFYVPAPLRKKKAEREEYRKSWSTATSPLGDRPFSKPHPETIEEEQNETSGLCEKEKVGSLAPEASSLKNQVGLNQNEGNSIDQSPSCSAKETSAPKGKDAEEIKKLRRLEQAGIKVMSAAQRYGSKKPTCEDNGSTPDIILRRDNPFMTHYQGKDSGSEDEAASRVPDLEKDDFATRRARMNQPKHIVPFNPFLIGPYTSKDKGKLEDIKKPLQMEKQEHARRRRVHMASTRQIVTSKENPLLQTEKTESEEEQQVKVPDTESDDLAKQKSQSTLPQLREDAIFVNASITKADLETWKRLKLSGEPSEEDFKCPSDTLPPAEITVKAAAHDDFAVRKARANKKAGSPKQKFVHFGPVTEIDQQKWDKLVIGRASSKNGTEEESKKDSVENHSAVESSLSTTVGSLGFAEHSDEVSKPQNESSRIVSEVATSGTDDSCRRMLSPVPAFGIVSEKQTLCSLNKRQEGTEEDCVGHLPDIERDDMLVRRMGTFQRRTTSPVPIHCPPLSVAPHLLQQRQGEAVACEKDLKTPWEAESTVTREESQEQPEIPQQQSGGTENEVKFPDPQRDDMLARRTGAFLKQPGPSVKQFLPMPFSKQQNKQGTARDLEKKNTRPEKSPQVCINSSGKPVVQPPQEQQQGQEKCNLPPAGQDETICTAQVGEGSLNEVHLGQMSKLQEVPEENKTETLSSKDEHLQTFGPRTAVSDDAESMSMFDMRCEEEAVTQPHSKARHEKLQNVHNQLKEDETRWQDDLARWKSRRRSASQDLIKKEAERKKMERLLSGDGTNERRKSIKTYREIVEEKERRERELHEAYKNAKSQEEAESILQQYIERFTISEAVLERLQMPKILERSHSVEPNSPLKDPNPLRYLRQQSLPAPKFTATVEATIVPTTEIEPSGSAGRTSPSKSIVSKAVPMLTPKPYSQPKNTQEVLKNFKVDGKVSMNGENFNGVEEKDKECTTVILTPSPSRSLKFDGVARGDRPPVELKKDPTSVELSLQRPATQSVHKEPTASLVEADRAASKQVEASPGSAGPVSPVCPCPGSDQKQFKSTAACASPVVKRLEFLPSPFPSEEAASSEKDVKKPEMEQQKEAELPAAPEVMKPKALEQEGSVVLPFLKKLPETSQVTLQNSGLQADSDSGDKSNRFNFWSWDPEEERKRQERWQHEQERLLQEKYQKEQDKLKEEWEKAQREVEEEERRYYEEERKIIEDTVVPFIVSSNSAELLSSSSSTTEGNKTANTTDSNSSPEEVKQKEVTRQKKLLWEQDSIPSPKSKENELWQEKRSGNKVLSGHPEMGILKGGEQEHQVPEMEHSSPARLNLPLTQDVSWNQQLLTKQSPHRAEDIRQRPFLSQSAGQQPSSAGETRRAGYHENFQSGPSSPCSPAAPSQSPNRYKRSVSGKKLCSTCGLPLGKGAAMIIETLGLYFHIQCFRCGICKGQLGDAASGTDVRIRNGLLNCTDCYIRSRTAGQPTTL

Target ORF information:

RefSeq Version	XM_019472302.1
Organism	Aptenodytes forsteri(emperor penguin)
Definition	Aptenodytes forsteri LIM and calponin homology domains 1 (LIMCH1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019472302.1

ORF Insert Sequence:

ATGAGAAAGG AAACGGAGGA TATTGAGAGC CCAAAGCGCA GCATTCGTGA CAGCGGCTAT 
ATAGACTGCT GGGACTCTGA ACGCAGCGAT TCCCTCTCCC CGCCTCGCCA CGGCAGAGAT 
GATTCCTTTG ACAGTCTGGA CTCCTTTGGC TCCCGCTCGC AGCAGACACC GTCTCCAGAT 
GTAGTGCTCA GAGGGAGCAG CGATGGGAGA GGAAGTGACT CGGAAACTGA CCTGCCACAC 
AGAAAGATGC CAGATGTGAA AAAAGATGAT ATGTTGGCGA GGCGGACCTC ACATGGTGAA 
CCTAAATCAG CTGTGCCTTT TAACCAGTAC CTCCCTAACA AGAGTAATCA GACTTTCTAT 
GTACCTGCTC CACTTCGGAA AAAGAAAGCT GAACGAGAAG AGTACCGAAA GAGCTGGAGC 
ACGGCGACTT CACCACTAGG AGACAGACCT TTCAGCAAAC CCCACCCTGA AACCATAGAA 
GAAGAACAGA ATGAAACATC AGGACTGTGT GAAAAGGAGA AAGTGGGCTC CCTGGCTCCT 
GAGGCAAGCT CCCTTAAAAA TCAAGTAGGT TTGAATCAAA ACGAAGGAAA CTCCATAGAC 
CAGTCTCCTT CATGTAGTGC AAAAGAAACA TCAGCACCCA AGGGGAAAGA TGCAGAAGAG 
ATCAAAAAGT TAAGAAGACT CGAACAAGCT GGCATTAAGG TCATGTCAGC AGCCCAGCGC 
TATGGCAGTA AAAAGCCAAC ATGTGAAGAC AACGGAAGCA CCCCAGACAT CATCCTTCGC 
AGGGACAACC CCTTCATGAC ACATTACCAA GGGAAGGATT CTGGTTCAGA AGATGAAGCA 
GCCAGCAGAG TTCCTGATTT AGAAAAGGAT GACTTTGCCA CCCGGAGAGC CAGAATGAAT 
CAACCCAAAC ACATAGTTCC GTTTAACCCC TTTTTAATTG GACCCTATAC AAGTAAAGAT 
AAAGGTAAAC TGGAAGACAT TAAAAAGCCA TTGCAGATGG AAAAGCAGGA ACATGCAAGA 
AGAAGACGGG TGCATATGGC ATCAACAAGG CAAATAGTGA CAAGCAAAGA GAACCCACTT 
CTGCAAACTG AGAAGACTGA GTCAGAGGAG GAACAGCAGG TGAAAGTGCC AGATACTGAG 
AGCGATGATT TGGCTAAACA GAAATCTCAA AGTACCCTTC CTCAGCTACG AGAAGATGCG 
ATTTTTGTGA ATGCCTCTAT AACAAAAGCA GATCTGGAGA CCTGGAAAAG ATTGAAACTG 
TCAGGGGAGC CCAGTGAAGA AGATTTCAAA TGTCCCAGTG ACACTCTGCC ACCAGCTGAA 
ATCACGGTGA AAGCAGCGGC CCACGATGAT TTTGCTGTCC GCAAAGCCAG AGCAAACAAA 
AAGGCTGGTA GCCCCAAGCA GAAGTTTGTG CATTTTGGGC CAGTAACTGA GATTGATCAA 
CAGAAATGGG ACAAGCTCGT TATTGGCAGA GCTTCATCCA AAAATGGAAC AGAAGAGGAA 
AGCAAGAAAG ACAGTGTGGA GAATCACTCT GCTGTTGAAA GCTCTCTTTC AACCACTGTT 
GGCAGTCTCG GGTTCGCTGA ACACAGCGAT GAAGTATCTA AGCCACAGAA TGAATCCAGT 
CGCATCGTTA GTGAGGTAGC CACGTCAGGT ACAGATGACT CTTGCAGGAG AATGCTATCA 
CCTGTCCCGG CTTTTGGCAT CGTCTCTGAG AAACAAACCC TGTGCAGTTT GAACAAACGG 
CAAGAAGGCA CCGAGGAAGA CTGCGTCGGG CATTTACCAG ACATTGAGAG AGATGATATG 
TTGGTTAGAA GGATGGGAAC TTTTCAGAGG CGTACCACTA GCCCTGTTCC CATACATTGT 
CCTCCCCTGT CAGTAGCTCC TCATCTGCTG CAGCAGCGGC AAGGGGAGGC AGTAGCTTGT 
GAGAAGGATT TGAAGACTCC ATGGGAAGCA GAAAGTACTG TCACACGAGA AGAGAGCCAA 
GAACAGCCTG AAATCCCTCA GCAGCAGAGC GGTGGAACAG AAAACGAAGT GAAATTTCCA 
GACCCGCAAA GAGATGACAT GTTGGCCAGA AGAACTGGAG CTTTCCTAAA ACAGCCTGGG 
CCCAGTGTTA AGCAGTTCCT TCCCATGCCA TTTTCAAAGC AGCAGAATAA GCAGGGCACA 
GCTAGAGATC TTGAGAAGAA AAATACGAGG CCTGAAAAGT CTCCACAAGT CTGCATTAAT 
AGCTCTGGAA AACCTGTAGT ACAACCGCCG CAAGAACAGC AGCAAGGGCA GGAAAAATGC 
AATCTCCCTC CTGCAGGACA AGATGAGACT ATTTGTACTG CCCAAGTGGG TGAAGGTAGC 
CTTAATGAAG TCCACCTTGG TCAAATGTCT AAGCTTCAGG AGGTTCCTGA GGAAAACAAA 
ACGGAAACAT TGTCATCCAA GGATGAGCAT CTGCAAACAT TCGGCCCCAG AACTGCTGTG 
TCTGATGACG CAGAGAGTAT GAGCATGTTT GACATGAGAT GTGAAGAAGA AGCTGTGACT 
CAGCCTCATA GCAAAGCTCG CCATGAGAAA CTGCAGAACG TACACAACCA GCTGAAAGAG 
GATGAGACCA GATGGCAAGA TGACCTGGCT CGGTGGAAGA GTCGTCGAAG GAGCGCTTCA 
CAGGATTTAA TAAAAAAAGA AGCTGAAAGG AAGAAAATGG AAAGGTTATT GTCTGGAGAT 
GGAACGAATG AGCGCAGAAA AAGCATCAAA ACCTACCGAG AAATTGTGGA AGAGAAAGAG 
AGAAGAGAGC GGGAGCTTCA TGAGGCATAC AAGAATGCAA AGTCACAAGA GGAGGCTGAA 
AGCATCCTCC AGCAGTACAT CGAAAGATTC ACTATCAGTG AAGCTGTCCT TGAGCGTTTG 
CAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAGTGGAGC CAAATTCCCC ACTGAAAGAC 
CCAAATCCTC TGAGATATTT AAGGCAACAG TCACTGCCTG CTCCAAAATT CACTGCCACC 
GTTGAAGCAA CTATTGTTCC CACCACTGAA ATAGAGCCCA GTGGTTCGGC GGGCCGCACA 
TCTCCCAGCA AAAGCATTGT CTCCAAGGCT GTGCCTATGC TGACACCCAA GCCTTACTCA 
CAACCCAAAA ATACACAGGA AGTTTTAAAG AACTTTAAGG TAGATGGAAA AGTCAGCATG 
AATGGAGAAA ATTTCAACGG TGTGGAAGAG AAGGATAAAG AGTGTACAAC AGTAATACTT 
ACTCCTTCGC CAAGCAGATC TCTGAAATTT GACGGAGTAG CCAGAGGGGA CAGGCCCCCG 
GTAGAGTTGA AGAAGGACCC CACAAGTGTT GAGCTCAGTT TACAGAGGCC TGCCACTCAG 
AGTGTGCACA AAGAGCCAAC AGCCTCACTG GTGGAAGCAG ATAGAGCTGC CAGCAAACAG 
GTCGAGGCTA GCCCTGGAAG TGCAGGACCT GTGAGCCCGG TGTGCCCGTG CCCAGGTTCA 
GATCAAAAGC AATTCAAGTC AACTGCAGCT TGTGCCAGCC CTGTTGTGAA GAGATTAGAA 
TTTCTTCCCA GTCCGTTCCC TTCAGAGGAA GCTGCTTCAA GTGAGAAAGA TGTGAAGAAA 
CCTGAAATGG AGCAGCAGAA GGAAGCAGAG CTTCCTGCTG CACCGGAGGT GATGAAACCA 
AAAGCCCTGG AACAAGAGGG GAGTGTAGTA TTGCCATTTT TAAAGAAACT ACCCGAGACC 
AGCCAAGTTA CTCTGCAGAA TTCTGGTCTA CAAGCTGATT CTGATAGTGG TGACAAATCA 
AATCGTTTCA ATTTCTGGTC CTGGGATCCA GAAGAAGAGC GAAAGCGTCA GGAAAGGTGG 
CAGCATGAGC AAGAACGCTT GCTTCAGGAG AAGTATCAGA AGGAGCAGGA CAAGCTGAAA 
GAGGAATGGG AAAAGGCCCA GAGAGAAGTT GAAGAGGAGG AGCGTAGATA CTATGAGGAG 
GAACGTAAGA TAATTGAGGA TACTGTAGTT CCATTCATTG TTTCTTCAAA CTCTGCTGAG 
CTCCTCTCCT CCTCTTCCTC TACCACTGAA GGAAACAAGA CAGCGAACAC AACTGATTCA 
AACAGCTCTC CAGAGGAAGT CAAACAAAAA GAAGTTACAA GGCAGAAAAA GTTGCTCTGG 
GAGCAGGACA GTATACCATC TCCGAAAAGC AAGGAAAATG AACTCTGGCA AGAAAAGAGG 
AGTGGAAATA AAGTGCTCTC AGGACACCCG GAGATGGGTA TCTTGAAAGG AGGTGAACAG 
GAGCACCAGG TGCCAGAGAT GGAGCACAGC TCGCCTGCCA GGCTGAATCT GCCGTTGACT 
CAGGACGTCT CCTGGAATCA GCAGTTGCTG ACAAAGCAGT CCCCACATCG TGCAGAGGAC 
ATTCGGCAGA GACCATTCCT GAGCCAGAGT GCGGGACAAC AGCCGAGCTC CGCAGGAGAA 
ACGAGGAGGG CAGGCTATCA TGAGAACTTC CAATCTGGGC CATCATCTCC CTGCTCTCCT 
GCTGCTCCGA GTCAGTCACC CAACAGGTAC AAAAGGTCTG TCAGTGGAAA GAAGCTTTGT 
TCTACCTGTG GGCTTCCTCT TGGGAAAGGA GCTGCCATGA TTATTGAAAC GCTTGGTCTT 
TATTTCCATA TTCAGTGCTT CAGGTGTGGC ATTTGCAAGG GACAGCTGGG AGACGCAGCA 
AGCGGGACAG ATGTCAGGAT TAGAAATGGT CTTCTTAACT GCACCGATTG TTATATCCGA 
TCCAGAACTG CTGGACAGCC AACTACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_019472300.1 ) cDNA ORF clone, Aptenodytes forsteri(emperor penguin) -> XP_019327845.1 Aptenodytes forsteri LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.

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CloneID	OAp53058
Clone ID Related Accession (Same CDS sequence)	XM_019472302.1 , XM_019472300.1 , XM_019472303.1
Accession Version	XM_019472300.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Aptenodytes forsteri(emperor penguin)
Product	LIM and calponin homology domains-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008795334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aptenodytes forsteri Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAGG AAACGGAGGA TATTGAGAGC CCAAAGCGCA GCATTCGTGA CAGCGGCTAT 
ATAGACTGCT GGGACTCTGA ACGCAGCGAT TCCCTCTCCC CGCCTCGCCA CGGCAGAGAT 
GATTCCTTTG ACAGTCTGGA CTCCTTTGGC TCCCGCTCGC AGCAGACACC GTCTCCAGAT 
GTAGTGCTCA GAGGGAGCAG CGATGGGAGA GGAAGTGACT CGGAAACTGA CCTGCCACAC 
AGAAAGATGC CAGATGTGAA AAAAGATGAT ATGTTGGCGA GGCGGACCTC ACATGGTGAA 
CCTAAATCAG CTGTGCCTTT TAACCAGTAC CTCCCTAACA AGAGTAATCA GACTTTCTAT 
GTACCTGCTC CACTTCGGAA AAAGAAAGCT GAACGAGAAG AGTACCGAAA GAGCTGGAGC 
ACGGCGACTT CACCACTAGG AGACAGACCT TTCAGCAAAC CCCACCCTGA AACCATAGAA 
GAAGAACAGA ATGAAACATC AGGACTGTGT GAAAAGGAGA AAGTGGGCTC CCTGGCTCCT 
GAGGCAAGCT CCCTTAAAAA TCAAGTAGGT TTGAATCAAA ACGAAGGAAA CTCCATAGAC 
CAGTCTCCTT CATGTAGTGC AAAAGAAACA TCAGCACCCA AGGGGAAAGA TGCAGAAGAG 
ATCAAAAAGT TAAGAAGACT CGAACAAGCT GGCATTAAGG TCATGTCAGC AGCCCAGCGC 
TATGGCAGTA AAAAGCCAAC ATGTGAAGAC AACGGAAGCA CCCCAGACAT CATCCTTCGC 
AGGGACAACC CCTTCATGAC ACATTACCAA GGGAAGGATT CTGGTTCAGA AGATGAAGCA 
GCCAGCAGAG TTCCTGATTT AGAAAAGGAT GACTTTGCCA CCCGGAGAGC CAGAATGAAT 
CAACCCAAAC ACATAGTTCC GTTTAACCCC TTTTTAATTG GACCCTATAC AAGTAAAGAT 
AAAGGTAAAC TGGAAGACAT TAAAAAGCCA TTGCAGATGG AAAAGCAGGA ACATGCAAGA 
AGAAGACGGG TGCATATGGC ATCAACAAGG CAAATAGTGA CAAGCAAAGA GAACCCACTT 
CTGCAAACTG AGAAGACTGA GTCAGAGGAG GAACAGCAGG TGAAAGTGCC AGATACTGAG 
AGCGATGATT TGGCTAAACA GAAATCTCAA AGTACCCTTC CTCAGCTACG AGAAGATGCG 
ATTTTTGTGA ATGCCTCTAT AACAAAAGCA GATCTGGAGA CCTGGAAAAG ATTGAAACTG 
TCAGGGGAGC CCAGTGAAGA AGATTTCAAA TGTCCCAGTG ACACTCTGCC ACCAGCTGAA 
ATCACGGTGA AAGCAGCGGC CCACGATGAT TTTGCTGTCC GCAAAGCCAG AGCAAACAAA 
AAGGCTGGTA GCCCCAAGCA GAAGTTTGTG CATTTTGGGC CAGTAACTGA GATTGATCAA 
CAGAAATGGG ACAAGCTCGT TATTGGCAGA GCTTCATCCA AAAATGGAAC AGAAGAGGAA 
AGCAAGAAAG ACAGTGTGGA GAATCACTCT GCTGTTGAAA GCTCTCTTTC AACCACTGTT 
GGCAGTCTCG GGTTCGCTGA ACACAGCGAT GAAGTATCTA AGCCACAGAA TGAATCCAGT 
CGCATCGTTA GTGAGGTAGC CACGTCAGGT ACAGATGACT CTTGCAGGAG AATGCTATCA 
CCTGTCCCGG CTTTTGGCAT CGTCTCTGAG AAACAAACCC TGTGCAGTTT GAACAAACGG 
CAAGAAGGCA CCGAGGAAGA CTGCGTCGGG CATTTACCAG ACATTGAGAG AGATGATATG 
TTGGTTAGAA GGATGGGAAC TTTTCAGAGG CGTACCACTA GCCCTGTTCC CATACATTGT 
CCTCCCCTGT CAGTAGCTCC TCATCTGCTG CAGCAGCGGC AAGGGGAGGC AGTAGCTTGT 
GAGAAGGATT TGAAGACTCC ATGGGAAGCA GAAAGTACTG TCACACGAGA AGAGAGCCAA 
GAACAGCCTG AAATCCCTCA GCAGCAGAGC GGTGGAACAG AAAACGAAGT GAAATTTCCA 
GACCCGCAAA GAGATGACAT GTTGGCCAGA AGAACTGGAG CTTTCCTAAA ACAGCCTGGG 
CCCAGTGTTA AGCAGTTCCT TCCCATGCCA TTTTCAAAGC AGCAGAATAA GCAGGGCACA 
GCTAGAGATC TTGAGAAGAA AAATACGAGG CCTGAAAAGT CTCCACAAGT CTGCATTAAT 
AGCTCTGGAA AACCTGTAGT ACAACCGCCG CAAGAACAGC AGCAAGGGCA GGAAAAATGC 
AATCTCCCTC CTGCAGGACA AGATGAGACT ATTTGTACTG CCCAAGTGGG TGAAGGTAGC 
CTTAATGAAG TCCACCTTGG TCAAATGTCT AAGCTTCAGG AGGTTCCTGA GGAAAACAAA 
ACGGAAACAT TGTCATCCAA GGATGAGCAT CTGCAAACAT TCGGCCCCAG AACTGCTGTG 
TCTGATGACG CAGAGAGTAT GAGCATGTTT GACATGAGAT GTGAAGAAGA AGCTGTGACT 
CAGCCTCATA GCAAAGCTCG CCATGAGAAA CTGCAGAACG TACACAACCA GCTGAAAGAG 
GATGAGACCA GATGGCAAGA TGACCTGGCT CGGTGGAAGA GTCGTCGAAG GAGCGCTTCA 
CAGGATTTAA TAAAAAAAGA AGCTGAAAGG AAGAAAATGG AAAGGTTATT GTCTGGAGAT 
GGAACGAATG AGCGCAGAAA AAGCATCAAA ACCTACCGAG AAATTGTGGA AGAGAAAGAG 
AGAAGAGAGC GGGAGCTTCA TGAGGCATAC AAGAATGCAA AGTCACAAGA GGAGGCTGAA 
AGCATCCTCC AGCAGTACAT CGAAAGATTC ACTATCAGTG AAGCTGTCCT TGAGCGTTTG 
CAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAGTGGAGC CAAATTCCCC ACTGAAAGAC 
CCAAATCCTC TGAGATATTT AAGGCAACAG TCACTGCCTG CTCCAAAATT CACTGCCACC 
GTTGAAGCAA CTATTGTTCC CACCACTGAA ATAGAGCCCA GTGGTTCGGC GGGCCGCACA 
TCTCCCAGCA AAAGCATTGT CTCCAAGGCT GTGCCTATGC TGACACCCAA GCCTTACTCA 
CAACCCAAAA ATACACAGGA AGTTTTAAAG AACTTTAAGG TAGATGGAAA AGTCAGCATG 
AATGGAGAAA ATTTCAACGG TGTGGAAGAG AAGGATAAAG AGTGTACAAC AGTAATACTT 
ACTCCTTCGC CAAGCAGATC TCTGAAATTT GACGGAGTAG CCAGAGGGGA CAGGCCCCCG 
GTAGAGTTGA AGAAGGACCC CACAAGTGTT GAGCTCAGTT TACAGAGGCC TGCCACTCAG 
AGTGTGCACA AAGAGCCAAC AGCCTCACTG GTGGAAGCAG ATAGAGCTGC CAGCAAACAG 
GTCGAGGCTA GCCCTGGAAG TGCAGGACCT GTGAGCCCGG TGTGCCCGTG CCCAGGTTCA 
GATCAAAAGC AATTCAAGTC AACTGCAGCT TGTGCCAGCC CTGTTGTGAA GAGATTAGAA 
TTTCTTCCCA GTCCGTTCCC TTCAGAGGAA GCTGCTTCAA GTGAGAAAGA TGTGAAGAAA 
CCTGAAATGG AGCAGCAGAA GGAAGCAGAG CTTCCTGCTG CACCGGAGGT GATGAAACCA 
AAAGCCCTGG AACAAGAGGG GAGTGTAGTA TTGCCATTTT TAAAGAAACT ACCCGAGACC 
AGCCAAGTTA CTCTGCAGAA TTCTGGTCTA CAAGCTGATT CTGATAGTGG TGACAAATCA 
AATCGTTTCA ATTTCTGGTC CTGGGATCCA GAAGAAGAGC GAAAGCGTCA GGAAAGGTGG 
CAGCATGAGC AAGAACGCTT GCTTCAGGAG AAGTATCAGA AGGAGCAGGA CAAGCTGAAA 
GAGGAATGGG AAAAGGCCCA GAGAGAAGTT GAAGAGGAGG AGCGTAGATA CTATGAGGAG 
GAACGTAAGA TAATTGAGGA TACTGTAGTT CCATTCATTG TTTCTTCAAA CTCTGCTGAG 
CTCCTCTCCT CCTCTTCCTC TACCACTGAA GGAAACAAGA CAGCGAACAC AACTGATTCA 
AACAGCTCTC CAGAGGAAGT CAAACAAAAA GAAGTTACAA GGCAGAAAAA GTTGCTCTGG 
GAGCAGGACA GTATACCATC TCCGAAAAGC AAGGAAAATG AACTCTGGCA AGAAAAGAGG 
AGTGGAAATA AAGTGCTCTC AGGACACCCG GAGATGGGTA TCTTGAAAGG AGGTGAACAG 
GAGCACCAGG TGCCAGAGAT GGAGCACAGC TCGCCTGCCA GGCTGAATCT GCCGTTGACT 
CAGGACGTCT CCTGGAATCA GCAGTTGCTG ACAAAGCAGT CCCCACATCG TGCAGAGGAC 
ATTCGGCAGA GACCATTCCT GAGCCAGAGT GCGGGACAAC AGCCGAGCTC CGCAGGAGAA 
ACGAGGAGGG CAGGCTATCA TGAGAACTTC CAATCTGGGC CATCATCTCC CTGCTCTCCT 
GCTGCTCCGA GTCAGTCACC CAACAGGTAC AAAAGGTCTG TCAGTGGAAA GAAGCTTTGT 
TCTACCTGTG GGCTTCCTCT TGGGAAAGGA GCTGCCATGA TTATTGAAAC GCTTGGTCTT 
TATTTCCATA TTCAGTGCTT CAGGTGTGGC ATTTGCAAGG GACAGCTGGG AGACGCAGCA 
AGCGGGACAG ATGTCAGGAT TAGAAATGGT CTTCTTAACT GCACCGATTG TTATATCCGA 
TCCAGAACTG CTGGACAGCC AACTACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019327845.1
CDS	241..5013
Translation	MRKETEDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSETDLPHRKMPDVKKDDMLARRTSHGEPKSAVPFNQYLPNKSNQTFYVPAPLRKKKAEREEYRKSWSTATSPLGDRPFSKPHPETIEEEQNETSGLCEKEKVGSLAPEASSLKNQVGLNQNEGNSIDQSPSCSAKETSAPKGKDAEEIKKLRRLEQAGIKVMSAAQRYGSKKPTCEDNGSTPDIILRRDNPFMTHYQGKDSGSEDEAASRVPDLEKDDFATRRARMNQPKHIVPFNPFLIGPYTSKDKGKLEDIKKPLQMEKQEHARRRRVHMASTRQIVTSKENPLLQTEKTESEEEQQVKVPDTESDDLAKQKSQSTLPQLREDAIFVNASITKADLETWKRLKLSGEPSEEDFKCPSDTLPPAEITVKAAAHDDFAVRKARANKKAGSPKQKFVHFGPVTEIDQQKWDKLVIGRASSKNGTEEESKKDSVENHSAVESSLSTTVGSLGFAEHSDEVSKPQNESSRIVSEVATSGTDDSCRRMLSPVPAFGIVSEKQTLCSLNKRQEGTEEDCVGHLPDIERDDMLVRRMGTFQRRTTSPVPIHCPPLSVAPHLLQQRQGEAVACEKDLKTPWEAESTVTREESQEQPEIPQQQSGGTENEVKFPDPQRDDMLARRTGAFLKQPGPSVKQFLPMPFSKQQNKQGTARDLEKKNTRPEKSPQVCINSSGKPVVQPPQEQQQGQEKCNLPPAGQDETICTAQVGEGSLNEVHLGQMSKLQEVPEENKTETLSSKDEHLQTFGPRTAVSDDAESMSMFDMRCEEEAVTQPHSKARHEKLQNVHNQLKEDETRWQDDLARWKSRRRSASQDLIKKEAERKKMERLLSGDGTNERRKSIKTYREIVEEKERRERELHEAYKNAKSQEEAESILQQYIERFTISEAVLERLQMPKILERSHSVEPNSPLKDPNPLRYLRQQSLPAPKFTATVEATIVPTTEIEPSGSAGRTSPSKSIVSKAVPMLTPKPYSQPKNTQEVLKNFKVDGKVSMNGENFNGVEEKDKECTTVILTPSPSRSLKFDGVARGDRPPVELKKDPTSVELSLQRPATQSVHKEPTASLVEADRAASKQVEASPGSAGPVSPVCPCPGSDQKQFKSTAACASPVVKRLEFLPSPFPSEEAASSEKDVKKPEMEQQKEAELPAAPEVMKPKALEQEGSVVLPFLKKLPETSQVTLQNSGLQADSDSGDKSNRFNFWSWDPEEERKRQERWQHEQERLLQEKYQKEQDKLKEEWEKAQREVEEEERRYYEEERKIIEDTVVPFIVSSNSAELLSSSSSTTEGNKTANTTDSNSSPEEVKQKEVTRQKKLLWEQDSIPSPKSKENELWQEKRSGNKVLSGHPEMGILKGGEQEHQVPEMEHSSPARLNLPLTQDVSWNQQLLTKQSPHRAEDIRQRPFLSQSAGQQPSSAGETRRAGYHENFQSGPSSPCSPAAPSQSPNRYKRSVSGKKLCSTCGLPLGKGAAMIIETLGLYFHIQCFRCGICKGQLGDAASGTDVRIRNGLLNCTDCYIRSRTAGQPTTL

Target ORF information:

RefSeq Version	XM_019472300.1
Organism	Aptenodytes forsteri(emperor penguin)
Definition	Aptenodytes forsteri LIM and calponin homology domains 1 (LIMCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019472300.1

ORF Insert Sequence:

ATGAGAAAGG AAACGGAGGA TATTGAGAGC CCAAAGCGCA GCATTCGTGA CAGCGGCTAT 
ATAGACTGCT GGGACTCTGA ACGCAGCGAT TCCCTCTCCC CGCCTCGCCA CGGCAGAGAT 
GATTCCTTTG ACAGTCTGGA CTCCTTTGGC TCCCGCTCGC AGCAGACACC GTCTCCAGAT 
GTAGTGCTCA GAGGGAGCAG CGATGGGAGA GGAAGTGACT CGGAAACTGA CCTGCCACAC 
AGAAAGATGC CAGATGTGAA AAAAGATGAT ATGTTGGCGA GGCGGACCTC ACATGGTGAA 
CCTAAATCAG CTGTGCCTTT TAACCAGTAC CTCCCTAACA AGAGTAATCA GACTTTCTAT 
GTACCTGCTC CACTTCGGAA AAAGAAAGCT GAACGAGAAG AGTACCGAAA GAGCTGGAGC 
ACGGCGACTT CACCACTAGG AGACAGACCT TTCAGCAAAC CCCACCCTGA AACCATAGAA 
GAAGAACAGA ATGAAACATC AGGACTGTGT GAAAAGGAGA AAGTGGGCTC CCTGGCTCCT 
GAGGCAAGCT CCCTTAAAAA TCAAGTAGGT TTGAATCAAA ACGAAGGAAA CTCCATAGAC 
CAGTCTCCTT CATGTAGTGC AAAAGAAACA TCAGCACCCA AGGGGAAAGA TGCAGAAGAG 
ATCAAAAAGT TAAGAAGACT CGAACAAGCT GGCATTAAGG TCATGTCAGC AGCCCAGCGC 
TATGGCAGTA AAAAGCCAAC ATGTGAAGAC AACGGAAGCA CCCCAGACAT CATCCTTCGC 
AGGGACAACC CCTTCATGAC ACATTACCAA GGGAAGGATT CTGGTTCAGA AGATGAAGCA 
GCCAGCAGAG TTCCTGATTT AGAAAAGGAT GACTTTGCCA CCCGGAGAGC CAGAATGAAT 
CAACCCAAAC ACATAGTTCC GTTTAACCCC TTTTTAATTG GACCCTATAC AAGTAAAGAT 
AAAGGTAAAC TGGAAGACAT TAAAAAGCCA TTGCAGATGG AAAAGCAGGA ACATGCAAGA 
AGAAGACGGG TGCATATGGC ATCAACAAGG CAAATAGTGA CAAGCAAAGA GAACCCACTT 
CTGCAAACTG AGAAGACTGA GTCAGAGGAG GAACAGCAGG TGAAAGTGCC AGATACTGAG 
AGCGATGATT TGGCTAAACA GAAATCTCAA AGTACCCTTC CTCAGCTACG AGAAGATGCG 
ATTTTTGTGA ATGCCTCTAT AACAAAAGCA GATCTGGAGA CCTGGAAAAG ATTGAAACTG 
TCAGGGGAGC CCAGTGAAGA AGATTTCAAA TGTCCCAGTG ACACTCTGCC ACCAGCTGAA 
ATCACGGTGA AAGCAGCGGC CCACGATGAT TTTGCTGTCC GCAAAGCCAG AGCAAACAAA 
AAGGCTGGTA GCCCCAAGCA GAAGTTTGTG CATTTTGGGC CAGTAACTGA GATTGATCAA 
CAGAAATGGG ACAAGCTCGT TATTGGCAGA GCTTCATCCA AAAATGGAAC AGAAGAGGAA 
AGCAAGAAAG ACAGTGTGGA GAATCACTCT GCTGTTGAAA GCTCTCTTTC AACCACTGTT 
GGCAGTCTCG GGTTCGCTGA ACACAGCGAT GAAGTATCTA AGCCACAGAA TGAATCCAGT 
CGCATCGTTA GTGAGGTAGC CACGTCAGGT ACAGATGACT CTTGCAGGAG AATGCTATCA 
CCTGTCCCGG CTTTTGGCAT CGTCTCTGAG AAACAAACCC TGTGCAGTTT GAACAAACGG 
CAAGAAGGCA CCGAGGAAGA CTGCGTCGGG CATTTACCAG ACATTGAGAG AGATGATATG 
TTGGTTAGAA GGATGGGAAC TTTTCAGAGG CGTACCACTA GCCCTGTTCC CATACATTGT 
CCTCCCCTGT CAGTAGCTCC TCATCTGCTG CAGCAGCGGC AAGGGGAGGC AGTAGCTTGT 
GAGAAGGATT TGAAGACTCC ATGGGAAGCA GAAAGTACTG TCACACGAGA AGAGAGCCAA 
GAACAGCCTG AAATCCCTCA GCAGCAGAGC GGTGGAACAG AAAACGAAGT GAAATTTCCA 
GACCCGCAAA GAGATGACAT GTTGGCCAGA AGAACTGGAG CTTTCCTAAA ACAGCCTGGG 
CCCAGTGTTA AGCAGTTCCT TCCCATGCCA TTTTCAAAGC AGCAGAATAA GCAGGGCACA 
GCTAGAGATC TTGAGAAGAA AAATACGAGG CCTGAAAAGT CTCCACAAGT CTGCATTAAT 
AGCTCTGGAA AACCTGTAGT ACAACCGCCG CAAGAACAGC AGCAAGGGCA GGAAAAATGC 
AATCTCCCTC CTGCAGGACA AGATGAGACT ATTTGTACTG CCCAAGTGGG TGAAGGTAGC 
CTTAATGAAG TCCACCTTGG TCAAATGTCT AAGCTTCAGG AGGTTCCTGA GGAAAACAAA 
ACGGAAACAT TGTCATCCAA GGATGAGCAT CTGCAAACAT TCGGCCCCAG AACTGCTGTG 
TCTGATGACG CAGAGAGTAT GAGCATGTTT GACATGAGAT GTGAAGAAGA AGCTGTGACT 
CAGCCTCATA GCAAAGCTCG CCATGAGAAA CTGCAGAACG TACACAACCA GCTGAAAGAG 
GATGAGACCA GATGGCAAGA TGACCTGGCT CGGTGGAAGA GTCGTCGAAG GAGCGCTTCA 
CAGGATTTAA TAAAAAAAGA AGCTGAAAGG AAGAAAATGG AAAGGTTATT GTCTGGAGAT 
GGAACGAATG AGCGCAGAAA AAGCATCAAA ACCTACCGAG AAATTGTGGA AGAGAAAGAG 
AGAAGAGAGC GGGAGCTTCA TGAGGCATAC AAGAATGCAA AGTCACAAGA GGAGGCTGAA 
AGCATCCTCC AGCAGTACAT CGAAAGATTC ACTATCAGTG AAGCTGTCCT TGAGCGTTTG 
CAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAGTGGAGC CAAATTCCCC ACTGAAAGAC 
CCAAATCCTC TGAGATATTT AAGGCAACAG TCACTGCCTG CTCCAAAATT CACTGCCACC 
GTTGAAGCAA CTATTGTTCC CACCACTGAA ATAGAGCCCA GTGGTTCGGC GGGCCGCACA 
TCTCCCAGCA AAAGCATTGT CTCCAAGGCT GTGCCTATGC TGACACCCAA GCCTTACTCA 
CAACCCAAAA ATACACAGGA AGTTTTAAAG AACTTTAAGG TAGATGGAAA AGTCAGCATG 
AATGGAGAAA ATTTCAACGG TGTGGAAGAG AAGGATAAAG AGTGTACAAC AGTAATACTT 
ACTCCTTCGC CAAGCAGATC TCTGAAATTT GACGGAGTAG CCAGAGGGGA CAGGCCCCCG 
GTAGAGTTGA AGAAGGACCC CACAAGTGTT GAGCTCAGTT TACAGAGGCC TGCCACTCAG 
AGTGTGCACA AAGAGCCAAC AGCCTCACTG GTGGAAGCAG ATAGAGCTGC CAGCAAACAG 
GTCGAGGCTA GCCCTGGAAG TGCAGGACCT GTGAGCCCGG TGTGCCCGTG CCCAGGTTCA 
GATCAAAAGC AATTCAAGTC AACTGCAGCT TGTGCCAGCC CTGTTGTGAA GAGATTAGAA 
TTTCTTCCCA GTCCGTTCCC TTCAGAGGAA GCTGCTTCAA GTGAGAAAGA TGTGAAGAAA 
CCTGAAATGG AGCAGCAGAA GGAAGCAGAG CTTCCTGCTG CACCGGAGGT GATGAAACCA 
AAAGCCCTGG AACAAGAGGG GAGTGTAGTA TTGCCATTTT TAAAGAAACT ACCCGAGACC 
AGCCAAGTTA CTCTGCAGAA TTCTGGTCTA CAAGCTGATT CTGATAGTGG TGACAAATCA 
AATCGTTTCA ATTTCTGGTC CTGGGATCCA GAAGAAGAGC GAAAGCGTCA GGAAAGGTGG 
CAGCATGAGC AAGAACGCTT GCTTCAGGAG AAGTATCAGA AGGAGCAGGA CAAGCTGAAA 
GAGGAATGGG AAAAGGCCCA GAGAGAAGTT GAAGAGGAGG AGCGTAGATA CTATGAGGAG 
GAACGTAAGA TAATTGAGGA TACTGTAGTT CCATTCATTG TTTCTTCAAA CTCTGCTGAG 
CTCCTCTCCT CCTCTTCCTC TACCACTGAA GGAAACAAGA CAGCGAACAC AACTGATTCA 
AACAGCTCTC CAGAGGAAGT CAAACAAAAA GAAGTTACAA GGCAGAAAAA GTTGCTCTGG 
GAGCAGGACA GTATACCATC TCCGAAAAGC AAGGAAAATG AACTCTGGCA AGAAAAGAGG 
AGTGGAAATA AAGTGCTCTC AGGACACCCG GAGATGGGTA TCTTGAAAGG AGGTGAACAG 
GAGCACCAGG TGCCAGAGAT GGAGCACAGC TCGCCTGCCA GGCTGAATCT GCCGTTGACT 
CAGGACGTCT CCTGGAATCA GCAGTTGCTG ACAAAGCAGT CCCCACATCG TGCAGAGGAC 
ATTCGGCAGA GACCATTCCT GAGCCAGAGT GCGGGACAAC AGCCGAGCTC CGCAGGAGAA 
ACGAGGAGGG CAGGCTATCA TGAGAACTTC CAATCTGGGC CATCATCTCC CTGCTCTCCT 
GCTGCTCCGA GTCAGTCACC CAACAGGTAC AAAAGGTCTG TCAGTGGAAA GAAGCTTTGT 
TCTACCTGTG GGCTTCCTCT TGGGAAAGGA GCTGCCATGA TTATTGAAAC GCTTGGTCTT 
TATTTCCATA TTCAGTGCTT CAGGTGTGGC ATTTGCAAGG GACAGCTGGG AGACGCAGCA 
AGCGGGACAG ATGTCAGGAT TAGAAATGGT CTTCTTAACT GCACCGATTG TTATATCCGA 
TCCAGAACTG CTGGACAGCC AACTACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_019472303.1 ) cDNA ORF clone, Aptenodytes forsteri(emperor penguin) -> XP_019327848.1 Aptenodytes forsteri LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.

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CloneID	OAp53058
Clone ID Related Accession (Same CDS sequence)	XM_019472302.1 , XM_019472300.1 , XM_019472303.1
Accession Version	XM_019472303.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Aptenodytes forsteri(emperor penguin)
Product	LIM and calponin homology domains-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008795334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aptenodytes forsteri Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAGG AAACGGAGGA TATTGAGAGC CCAAAGCGCA GCATTCGTGA CAGCGGCTAT 
ATAGACTGCT GGGACTCTGA ACGCAGCGAT TCCCTCTCCC CGCCTCGCCA CGGCAGAGAT 
GATTCCTTTG ACAGTCTGGA CTCCTTTGGC TCCCGCTCGC AGCAGACACC GTCTCCAGAT 
GTAGTGCTCA GAGGGAGCAG CGATGGGAGA GGAAGTGACT CGGAAACTGA CCTGCCACAC 
AGAAAGATGC CAGATGTGAA AAAAGATGAT ATGTTGGCGA GGCGGACCTC ACATGGTGAA 
CCTAAATCAG CTGTGCCTTT TAACCAGTAC CTCCCTAACA AGAGTAATCA GACTTTCTAT 
GTACCTGCTC CACTTCGGAA AAAGAAAGCT GAACGAGAAG AGTACCGAAA GAGCTGGAGC 
ACGGCGACTT CACCACTAGG AGACAGACCT TTCAGCAAAC CCCACCCTGA AACCATAGAA 
GAAGAACAGA ATGAAACATC AGGACTGTGT GAAAAGGAGA AAGTGGGCTC CCTGGCTCCT 
GAGGCAAGCT CCCTTAAAAA TCAAGTAGGT TTGAATCAAA ACGAAGGAAA CTCCATAGAC 
CAGTCTCCTT CATGTAGTGC AAAAGAAACA TCAGCACCCA AGGGGAAAGA TGCAGAAGAG 
ATCAAAAAGT TAAGAAGACT CGAACAAGCT GGCATTAAGG TCATGTCAGC AGCCCAGCGC 
TATGGCAGTA AAAAGCCAAC ATGTGAAGAC AACGGAAGCA CCCCAGACAT CATCCTTCGC 
AGGGACAACC CCTTCATGAC ACATTACCAA GGGAAGGATT CTGGTTCAGA AGATGAAGCA 
GCCAGCAGAG TTCCTGATTT AGAAAAGGAT GACTTTGCCA CCCGGAGAGC CAGAATGAAT 
CAACCCAAAC ACATAGTTCC GTTTAACCCC TTTTTAATTG GACCCTATAC AAGTAAAGAT 
AAAGGTAAAC TGGAAGACAT TAAAAAGCCA TTGCAGATGG AAAAGCAGGA ACATGCAAGA 
AGAAGACGGG TGCATATGGC ATCAACAAGG CAAATAGTGA CAAGCAAAGA GAACCCACTT 
CTGCAAACTG AGAAGACTGA GTCAGAGGAG GAACAGCAGG TGAAAGTGCC AGATACTGAG 
AGCGATGATT TGGCTAAACA GAAATCTCAA AGTACCCTTC CTCAGCTACG AGAAGATGCG 
ATTTTTGTGA ATGCCTCTAT AACAAAAGCA GATCTGGAGA CCTGGAAAAG ATTGAAACTG 
TCAGGGGAGC CCAGTGAAGA AGATTTCAAA TGTCCCAGTG ACACTCTGCC ACCAGCTGAA 
ATCACGGTGA AAGCAGCGGC CCACGATGAT TTTGCTGTCC GCAAAGCCAG AGCAAACAAA 
AAGGCTGGTA GCCCCAAGCA GAAGTTTGTG CATTTTGGGC CAGTAACTGA GATTGATCAA 
CAGAAATGGG ACAAGCTCGT TATTGGCAGA GCTTCATCCA AAAATGGAAC AGAAGAGGAA 
AGCAAGAAAG ACAGTGTGGA GAATCACTCT GCTGTTGAAA GCTCTCTTTC AACCACTGTT 
GGCAGTCTCG GGTTCGCTGA ACACAGCGAT GAAGTATCTA AGCCACAGAA TGAATCCAGT 
CGCATCGTTA GTGAGGTAGC CACGTCAGGT ACAGATGACT CTTGCAGGAG AATGCTATCA 
CCTGTCCCGG CTTTTGGCAT CGTCTCTGAG AAACAAACCC TGTGCAGTTT GAACAAACGG 
CAAGAAGGCA CCGAGGAAGA CTGCGTCGGG CATTTACCAG ACATTGAGAG AGATGATATG 
TTGGTTAGAA GGATGGGAAC TTTTCAGAGG CGTACCACTA GCCCTGTTCC CATACATTGT 
CCTCCCCTGT CAGTAGCTCC TCATCTGCTG CAGCAGCGGC AAGGGGAGGC AGTAGCTTGT 
GAGAAGGATT TGAAGACTCC ATGGGAAGCA GAAAGTACTG TCACACGAGA AGAGAGCCAA 
GAACAGCCTG AAATCCCTCA GCAGCAGAGC GGTGGAACAG AAAACGAAGT GAAATTTCCA 
GACCCGCAAA GAGATGACAT GTTGGCCAGA AGAACTGGAG CTTTCCTAAA ACAGCCTGGG 
CCCAGTGTTA AGCAGTTCCT TCCCATGCCA TTTTCAAAGC AGCAGAATAA GCAGGGCACA 
GCTAGAGATC TTGAGAAGAA AAATACGAGG CCTGAAAAGT CTCCACAAGT CTGCATTAAT 
AGCTCTGGAA AACCTGTAGT ACAACCGCCG CAAGAACAGC AGCAAGGGCA GGAAAAATGC 
AATCTCCCTC CTGCAGGACA AGATGAGACT ATTTGTACTG CCCAAGTGGG TGAAGGTAGC 
CTTAATGAAG TCCACCTTGG TCAAATGTCT AAGCTTCAGG AGGTTCCTGA GGAAAACAAA 
ACGGAAACAT TGTCATCCAA GGATGAGCAT CTGCAAACAT TCGGCCCCAG AACTGCTGTG 
TCTGATGACG CAGAGAGTAT GAGCATGTTT GACATGAGAT GTGAAGAAGA AGCTGTGACT 
CAGCCTCATA GCAAAGCTCG CCATGAGAAA CTGCAGAACG TACACAACCA GCTGAAAGAG 
GATGAGACCA GATGGCAAGA TGACCTGGCT CGGTGGAAGA GTCGTCGAAG GAGCGCTTCA 
CAGGATTTAA TAAAAAAAGA AGCTGAAAGG AAGAAAATGG AAAGGTTATT GTCTGGAGAT 
GGAACGAATG AGCGCAGAAA AAGCATCAAA ACCTACCGAG AAATTGTGGA AGAGAAAGAG 
AGAAGAGAGC GGGAGCTTCA TGAGGCATAC AAGAATGCAA AGTCACAAGA GGAGGCTGAA 
AGCATCCTCC AGCAGTACAT CGAAAGATTC ACTATCAGTG AAGCTGTCCT TGAGCGTTTG 
CAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAGTGGAGC CAAATTCCCC ACTGAAAGAC 
CCAAATCCTC TGAGATATTT AAGGCAACAG TCACTGCCTG CTCCAAAATT CACTGCCACC 
GTTGAAGCAA CTATTGTTCC CACCACTGAA ATAGAGCCCA GTGGTTCGGC GGGCCGCACA 
TCTCCCAGCA AAAGCATTGT CTCCAAGGCT GTGCCTATGC TGACACCCAA GCCTTACTCA 
CAACCCAAAA ATACACAGGA AGTTTTAAAG AACTTTAAGG TAGATGGAAA AGTCAGCATG 
AATGGAGAAA ATTTCAACGG TGTGGAAGAG AAGGATAAAG AGTGTACAAC AGTAATACTT 
ACTCCTTCGC CAAGCAGATC TCTGAAATTT GACGGAGTAG CCAGAGGGGA CAGGCCCCCG 
GTAGAGTTGA AGAAGGACCC CACAAGTGTT GAGCTCAGTT TACAGAGGCC TGCCACTCAG 
AGTGTGCACA AAGAGCCAAC AGCCTCACTG GTGGAAGCAG ATAGAGCTGC CAGCAAACAG 
GTCGAGGCTA GCCCTGGAAG TGCAGGACCT GTGAGCCCGG TGTGCCCGTG CCCAGGTTCA 
GATCAAAAGC AATTCAAGTC AACTGCAGCT TGTGCCAGCC CTGTTGTGAA GAGATTAGAA 
TTTCTTCCCA GTCCGTTCCC TTCAGAGGAA GCTGCTTCAA GTGAGAAAGA TGTGAAGAAA 
CCTGAAATGG AGCAGCAGAA GGAAGCAGAG CTTCCTGCTG CACCGGAGGT GATGAAACCA 
AAAGCCCTGG AACAAGAGGG GAGTGTAGTA TTGCCATTTT TAAAGAAACT ACCCGAGACC 
AGCCAAGTTA CTCTGCAGAA TTCTGGTCTA CAAGCTGATT CTGATAGTGG TGACAAATCA 
AATCGTTTCA ATTTCTGGTC CTGGGATCCA GAAGAAGAGC GAAAGCGTCA GGAAAGGTGG 
CAGCATGAGC AAGAACGCTT GCTTCAGGAG AAGTATCAGA AGGAGCAGGA CAAGCTGAAA 
GAGGAATGGG AAAAGGCCCA GAGAGAAGTT GAAGAGGAGG AGCGTAGATA CTATGAGGAG 
GAACGTAAGA TAATTGAGGA TACTGTAGTT CCATTCATTG TTTCTTCAAA CTCTGCTGAG 
CTCCTCTCCT CCTCTTCCTC TACCACTGAA GGAAACAAGA CAGCGAACAC AACTGATTCA 
AACAGCTCTC CAGAGGAAGT CAAACAAAAA GAAGTTACAA GGCAGAAAAA GTTGCTCTGG 
GAGCAGGACA GTATACCATC TCCGAAAAGC AAGGAAAATG AACTCTGGCA AGAAAAGAGG 
AGTGGAAATA AAGTGCTCTC AGGACACCCG GAGATGGGTA TCTTGAAAGG AGGTGAACAG 
GAGCACCAGG TGCCAGAGAT GGAGCACAGC TCGCCTGCCA GGCTGAATCT GCCGTTGACT 
CAGGACGTCT CCTGGAATCA GCAGTTGCTG ACAAAGCAGT CCCCACATCG TGCAGAGGAC 
ATTCGGCAGA GACCATTCCT GAGCCAGAGT GCGGGACAAC AGCCGAGCTC CGCAGGAGAA 
ACGAGGAGGG CAGGCTATCA TGAGAACTTC CAATCTGGGC CATCATCTCC CTGCTCTCCT 
GCTGCTCCGA GTCAGTCACC CAACAGGTAC AAAAGGTCTG TCAGTGGAAA GAAGCTTTGT 
TCTACCTGTG GGCTTCCTCT TGGGAAAGGA GCTGCCATGA TTATTGAAAC GCTTGGTCTT 
TATTTCCATA TTCAGTGCTT CAGGTGTGGC ATTTGCAAGG GACAGCTGGG AGACGCAGCA 
AGCGGGACAG ATGTCAGGAT TAGAAATGGT CTTCTTAACT GCACCGATTG TTATATCCGA 
TCCAGAACTG CTGGACAGCC AACTACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019327848.1
CDS	307..5079
Translation	MRKETEDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSETDLPHRKMPDVKKDDMLARRTSHGEPKSAVPFNQYLPNKSNQTFYVPAPLRKKKAEREEYRKSWSTATSPLGDRPFSKPHPETIEEEQNETSGLCEKEKVGSLAPEASSLKNQVGLNQNEGNSIDQSPSCSAKETSAPKGKDAEEIKKLRRLEQAGIKVMSAAQRYGSKKPTCEDNGSTPDIILRRDNPFMTHYQGKDSGSEDEAASRVPDLEKDDFATRRARMNQPKHIVPFNPFLIGPYTSKDKGKLEDIKKPLQMEKQEHARRRRVHMASTRQIVTSKENPLLQTEKTESEEEQQVKVPDTESDDLAKQKSQSTLPQLREDAIFVNASITKADLETWKRLKLSGEPSEEDFKCPSDTLPPAEITVKAAAHDDFAVRKARANKKAGSPKQKFVHFGPVTEIDQQKWDKLVIGRASSKNGTEEESKKDSVENHSAVESSLSTTVGSLGFAEHSDEVSKPQNESSRIVSEVATSGTDDSCRRMLSPVPAFGIVSEKQTLCSLNKRQEGTEEDCVGHLPDIERDDMLVRRMGTFQRRTTSPVPIHCPPLSVAPHLLQQRQGEAVACEKDLKTPWEAESTVTREESQEQPEIPQQQSGGTENEVKFPDPQRDDMLARRTGAFLKQPGPSVKQFLPMPFSKQQNKQGTARDLEKKNTRPEKSPQVCINSSGKPVVQPPQEQQQGQEKCNLPPAGQDETICTAQVGEGSLNEVHLGQMSKLQEVPEENKTETLSSKDEHLQTFGPRTAVSDDAESMSMFDMRCEEEAVTQPHSKARHEKLQNVHNQLKEDETRWQDDLARWKSRRRSASQDLIKKEAERKKMERLLSGDGTNERRKSIKTYREIVEEKERRERELHEAYKNAKSQEEAESILQQYIERFTISEAVLERLQMPKILERSHSVEPNSPLKDPNPLRYLRQQSLPAPKFTATVEATIVPTTEIEPSGSAGRTSPSKSIVSKAVPMLTPKPYSQPKNTQEVLKNFKVDGKVSMNGENFNGVEEKDKECTTVILTPSPSRSLKFDGVARGDRPPVELKKDPTSVELSLQRPATQSVHKEPTASLVEADRAASKQVEASPGSAGPVSPVCPCPGSDQKQFKSTAACASPVVKRLEFLPSPFPSEEAASSEKDVKKPEMEQQKEAELPAAPEVMKPKALEQEGSVVLPFLKKLPETSQVTLQNSGLQADSDSGDKSNRFNFWSWDPEEERKRQERWQHEQERLLQEKYQKEQDKLKEEWEKAQREVEEEERRYYEEERKIIEDTVVPFIVSSNSAELLSSSSSTTEGNKTANTTDSNSSPEEVKQKEVTRQKKLLWEQDSIPSPKSKENELWQEKRSGNKVLSGHPEMGILKGGEQEHQVPEMEHSSPARLNLPLTQDVSWNQQLLTKQSPHRAEDIRQRPFLSQSAGQQPSSAGETRRAGYHENFQSGPSSPCSPAAPSQSPNRYKRSVSGKKLCSTCGLPLGKGAAMIIETLGLYFHIQCFRCGICKGQLGDAASGTDVRIRNGLLNCTDCYIRSRTAGQPTTL

Target ORF information:

RefSeq Version	XM_019472303.1
Organism	Aptenodytes forsteri(emperor penguin)
Definition	Aptenodytes forsteri LIM and calponin homology domains 1 (LIMCH1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019472303.1

ORF Insert Sequence:

ATGAGAAAGG AAACGGAGGA TATTGAGAGC CCAAAGCGCA GCATTCGTGA CAGCGGCTAT 
ATAGACTGCT GGGACTCTGA ACGCAGCGAT TCCCTCTCCC CGCCTCGCCA CGGCAGAGAT 
GATTCCTTTG ACAGTCTGGA CTCCTTTGGC TCCCGCTCGC AGCAGACACC GTCTCCAGAT 
GTAGTGCTCA GAGGGAGCAG CGATGGGAGA GGAAGTGACT CGGAAACTGA CCTGCCACAC 
AGAAAGATGC CAGATGTGAA AAAAGATGAT ATGTTGGCGA GGCGGACCTC ACATGGTGAA 
CCTAAATCAG CTGTGCCTTT TAACCAGTAC CTCCCTAACA AGAGTAATCA GACTTTCTAT 
GTACCTGCTC CACTTCGGAA AAAGAAAGCT GAACGAGAAG AGTACCGAAA GAGCTGGAGC 
ACGGCGACTT CACCACTAGG AGACAGACCT TTCAGCAAAC CCCACCCTGA AACCATAGAA 
GAAGAACAGA ATGAAACATC AGGACTGTGT GAAAAGGAGA AAGTGGGCTC CCTGGCTCCT 
GAGGCAAGCT CCCTTAAAAA TCAAGTAGGT TTGAATCAAA ACGAAGGAAA CTCCATAGAC 
CAGTCTCCTT CATGTAGTGC AAAAGAAACA TCAGCACCCA AGGGGAAAGA TGCAGAAGAG 
ATCAAAAAGT TAAGAAGACT CGAACAAGCT GGCATTAAGG TCATGTCAGC AGCCCAGCGC 
TATGGCAGTA AAAAGCCAAC ATGTGAAGAC AACGGAAGCA CCCCAGACAT CATCCTTCGC 
AGGGACAACC CCTTCATGAC ACATTACCAA GGGAAGGATT CTGGTTCAGA AGATGAAGCA 
GCCAGCAGAG TTCCTGATTT AGAAAAGGAT GACTTTGCCA CCCGGAGAGC CAGAATGAAT 
CAACCCAAAC ACATAGTTCC GTTTAACCCC TTTTTAATTG GACCCTATAC AAGTAAAGAT 
AAAGGTAAAC TGGAAGACAT TAAAAAGCCA TTGCAGATGG AAAAGCAGGA ACATGCAAGA 
AGAAGACGGG TGCATATGGC ATCAACAAGG CAAATAGTGA CAAGCAAAGA GAACCCACTT 
CTGCAAACTG AGAAGACTGA GTCAGAGGAG GAACAGCAGG TGAAAGTGCC AGATACTGAG 
AGCGATGATT TGGCTAAACA GAAATCTCAA AGTACCCTTC CTCAGCTACG AGAAGATGCG 
ATTTTTGTGA ATGCCTCTAT AACAAAAGCA GATCTGGAGA CCTGGAAAAG ATTGAAACTG 
TCAGGGGAGC CCAGTGAAGA AGATTTCAAA TGTCCCAGTG ACACTCTGCC ACCAGCTGAA 
ATCACGGTGA AAGCAGCGGC CCACGATGAT TTTGCTGTCC GCAAAGCCAG AGCAAACAAA 
AAGGCTGGTA GCCCCAAGCA GAAGTTTGTG CATTTTGGGC CAGTAACTGA GATTGATCAA 
CAGAAATGGG ACAAGCTCGT TATTGGCAGA GCTTCATCCA AAAATGGAAC AGAAGAGGAA 
AGCAAGAAAG ACAGTGTGGA GAATCACTCT GCTGTTGAAA GCTCTCTTTC AACCACTGTT 
GGCAGTCTCG GGTTCGCTGA ACACAGCGAT GAAGTATCTA AGCCACAGAA TGAATCCAGT 
CGCATCGTTA GTGAGGTAGC CACGTCAGGT ACAGATGACT CTTGCAGGAG AATGCTATCA 
CCTGTCCCGG CTTTTGGCAT CGTCTCTGAG AAACAAACCC TGTGCAGTTT GAACAAACGG 
CAAGAAGGCA CCGAGGAAGA CTGCGTCGGG CATTTACCAG ACATTGAGAG AGATGATATG 
TTGGTTAGAA GGATGGGAAC TTTTCAGAGG CGTACCACTA GCCCTGTTCC CATACATTGT 
CCTCCCCTGT CAGTAGCTCC TCATCTGCTG CAGCAGCGGC AAGGGGAGGC AGTAGCTTGT 
GAGAAGGATT TGAAGACTCC ATGGGAAGCA GAAAGTACTG TCACACGAGA AGAGAGCCAA 
GAACAGCCTG AAATCCCTCA GCAGCAGAGC GGTGGAACAG AAAACGAAGT GAAATTTCCA 
GACCCGCAAA GAGATGACAT GTTGGCCAGA AGAACTGGAG CTTTCCTAAA ACAGCCTGGG 
CCCAGTGTTA AGCAGTTCCT TCCCATGCCA TTTTCAAAGC AGCAGAATAA GCAGGGCACA 
GCTAGAGATC TTGAGAAGAA AAATACGAGG CCTGAAAAGT CTCCACAAGT CTGCATTAAT 
AGCTCTGGAA AACCTGTAGT ACAACCGCCG CAAGAACAGC AGCAAGGGCA GGAAAAATGC 
AATCTCCCTC CTGCAGGACA AGATGAGACT ATTTGTACTG CCCAAGTGGG TGAAGGTAGC 
CTTAATGAAG TCCACCTTGG TCAAATGTCT AAGCTTCAGG AGGTTCCTGA GGAAAACAAA 
ACGGAAACAT TGTCATCCAA GGATGAGCAT CTGCAAACAT TCGGCCCCAG AACTGCTGTG 
TCTGATGACG CAGAGAGTAT GAGCATGTTT GACATGAGAT GTGAAGAAGA AGCTGTGACT 
CAGCCTCATA GCAAAGCTCG CCATGAGAAA CTGCAGAACG TACACAACCA GCTGAAAGAG 
GATGAGACCA GATGGCAAGA TGACCTGGCT CGGTGGAAGA GTCGTCGAAG GAGCGCTTCA 
CAGGATTTAA TAAAAAAAGA AGCTGAAAGG AAGAAAATGG AAAGGTTATT GTCTGGAGAT 
GGAACGAATG AGCGCAGAAA AAGCATCAAA ACCTACCGAG AAATTGTGGA AGAGAAAGAG 
AGAAGAGAGC GGGAGCTTCA TGAGGCATAC AAGAATGCAA AGTCACAAGA GGAGGCTGAA 
AGCATCCTCC AGCAGTACAT CGAAAGATTC ACTATCAGTG AAGCTGTCCT TGAGCGTTTG 
CAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAGTGGAGC CAAATTCCCC ACTGAAAGAC 
CCAAATCCTC TGAGATATTT AAGGCAACAG TCACTGCCTG CTCCAAAATT CACTGCCACC 
GTTGAAGCAA CTATTGTTCC CACCACTGAA ATAGAGCCCA GTGGTTCGGC GGGCCGCACA 
TCTCCCAGCA AAAGCATTGT CTCCAAGGCT GTGCCTATGC TGACACCCAA GCCTTACTCA 
CAACCCAAAA ATACACAGGA AGTTTTAAAG AACTTTAAGG TAGATGGAAA AGTCAGCATG 
AATGGAGAAA ATTTCAACGG TGTGGAAGAG AAGGATAAAG AGTGTACAAC AGTAATACTT 
ACTCCTTCGC CAAGCAGATC TCTGAAATTT GACGGAGTAG CCAGAGGGGA CAGGCCCCCG 
GTAGAGTTGA AGAAGGACCC CACAAGTGTT GAGCTCAGTT TACAGAGGCC TGCCACTCAG 
AGTGTGCACA AAGAGCCAAC AGCCTCACTG GTGGAAGCAG ATAGAGCTGC CAGCAAACAG 
GTCGAGGCTA GCCCTGGAAG TGCAGGACCT GTGAGCCCGG TGTGCCCGTG CCCAGGTTCA 
GATCAAAAGC AATTCAAGTC AACTGCAGCT TGTGCCAGCC CTGTTGTGAA GAGATTAGAA 
TTTCTTCCCA GTCCGTTCCC TTCAGAGGAA GCTGCTTCAA GTGAGAAAGA TGTGAAGAAA 
CCTGAAATGG AGCAGCAGAA GGAAGCAGAG CTTCCTGCTG CACCGGAGGT GATGAAACCA 
AAAGCCCTGG AACAAGAGGG GAGTGTAGTA TTGCCATTTT TAAAGAAACT ACCCGAGACC 
AGCCAAGTTA CTCTGCAGAA TTCTGGTCTA CAAGCTGATT CTGATAGTGG TGACAAATCA 
AATCGTTTCA ATTTCTGGTC CTGGGATCCA GAAGAAGAGC GAAAGCGTCA GGAAAGGTGG 
CAGCATGAGC AAGAACGCTT GCTTCAGGAG AAGTATCAGA AGGAGCAGGA CAAGCTGAAA 
GAGGAATGGG AAAAGGCCCA GAGAGAAGTT GAAGAGGAGG AGCGTAGATA CTATGAGGAG 
GAACGTAAGA TAATTGAGGA TACTGTAGTT CCATTCATTG TTTCTTCAAA CTCTGCTGAG 
CTCCTCTCCT CCTCTTCCTC TACCACTGAA GGAAACAAGA CAGCGAACAC AACTGATTCA 
AACAGCTCTC CAGAGGAAGT CAAACAAAAA GAAGTTACAA GGCAGAAAAA GTTGCTCTGG 
GAGCAGGACA GTATACCATC TCCGAAAAGC AAGGAAAATG AACTCTGGCA AGAAAAGAGG 
AGTGGAAATA AAGTGCTCTC AGGACACCCG GAGATGGGTA TCTTGAAAGG AGGTGAACAG 
GAGCACCAGG TGCCAGAGAT GGAGCACAGC TCGCCTGCCA GGCTGAATCT GCCGTTGACT 
CAGGACGTCT CCTGGAATCA GCAGTTGCTG ACAAAGCAGT CCCCACATCG TGCAGAGGAC 
ATTCGGCAGA GACCATTCCT GAGCCAGAGT GCGGGACAAC AGCCGAGCTC CGCAGGAGAA 
ACGAGGAGGG CAGGCTATCA TGAGAACTTC CAATCTGGGC CATCATCTCC CTGCTCTCCT 
GCTGCTCCGA GTCAGTCACC CAACAGGTAC AAAAGGTCTG TCAGTGGAAA GAAGCTTTGT 
TCTACCTGTG GGCTTCCTCT TGGGAAAGGA GCTGCCATGA TTATTGAAAC GCTTGGTCTT 
TATTTCCATA TTCAGTGCTT CAGGTGTGGC ATTTGCAAGG GACAGCTGGG AGACGCAGCA 
AGCGGGACAG ATGTCAGGAT TAGAAATGGT CTTCTTAACT GCACCGATTG TTATATCCGA 
TCCAGAACTG CTGGACAGCC AACTACATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_019472301.1 ) cDNA ORF clone, Aptenodytes forsteri(emperor penguin) -> XP_019327846.1 Aptenodytes forsteri LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.

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CloneID	OAp53059
Clone ID Related Accession (Same CDS sequence)	XM_019472301.1
Accession Version	XM_019472301.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Aptenodytes forsteri(emperor penguin)
Product	LIM and calponin homology domains-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008795334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aptenodytes forsteri Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAGG AAACGGAGGA TATTGAGAGC CCAAAGCGCA GCATTCGTGA CAGCGGCTAT 
ATAGACTGCT GGGACTCTGA ACGCAGCGAT TCCCTCTCCC CGCCTCGCCA CGGCAGAGAT 
GATTCCTTTG ACAGTCTGGA CTCCTTTGGC TCCCGCTCGC AGCAGACACC GTCTCCAGAT 
GTAGTGCTCA GAGGGAGCAG CGATGGGAGA GGAAGTGACT CGGAAACTGA CCTGCCACAC 
AGAAAGATGC CAGATGTGAA AAAAGATGAT ATGTTGGCGA GGCGGACCTC ACATGGTGAA 
CCTAAATCAG CTGTGCCTTT TAACCAGTAC CTCCCTAACA AGAGTAATCA GACTTTCTAT 
GTACCTGCTC CACTTCGGAA AAAGAAAGCT GAACGAGAAG AGTACCGAAA GAGCTGGAGC 
ACGGCGACTT CACCACTAGG AGACAGACCT TTCAGCAAAC CCCACCCTGA AACCATAGAA 
GAAGAACAGA ATGAAACATC AGGACTGTGT GAAAAGGAGA AAGTGGGCTC CCTGGCTCCT 
GAGGCAAGCT CCCTTAAAAA TCAAGTAGGT TTGAATCAAA ACGAAGGAAA CTCCATAGAC 
CAGTCTCCTT CATGTAGTGC AAAAGAAACA TCAGCACCCA AGGGGAAAGA TGCAGAAGAG 
ATCAAAAAGT TAAGAAGACT CGAACAAGCT GGCATTAAGG TCATGTCAGC AGCCCAGCGC 
TATGGCAGTA AAAAGCCAAC ATGTGAAGAC AACGGAAGCA CCCCAGACAT CATCCTTCGC 
AGGGACAACC CCTTCATGAC ACATTACCAA GGGAAGGATT CTGGTTCAGA AGATGAAGCA 
GCCAGCAGAG TTCCTGATTT AGAAAAGGAT GACTTTGCCA CCCGGAGAGC CAGAATGAAT 
CAACCCAAAC ACATAGTTCC GTTTAACCCC TTTTTAATTG GACCCTATAC AAGTAAAGAT 
AAAGGTAAAC TGGAAGACAT TAAAAAGCCA TTGCAGATGG AAAAGCAGGA ACATGCAAGA 
AGAAGACGGG TGCATATGGC ATCAACAAGG CAAATAGTGA CAAGCAAAGA GAACCCACTT 
CTGCAAACTG AGAAGACTGA GTCAGAGGAG GAACAGCAGG TGAAAGTGCC AGATACTGAG 
AGCGATGATT TGGCTAAACA GAAATCTCAA AGTACCCTTC CTCAGCTACG AGAAGATGCG 
ATTTTTGTGA ATGCCTCTAT AACAAAAGCA GATCTGGAGA CCTGGAAAAG ATTGAAACTG 
TCAGGGGAGC CCAGTGAAGA AGATTTCAAA TGTCCCAGTG ACACTCTGCC ACCAGCTGAA 
ATCACGGTGA AAGCAGCGGC CCACGATGAT TTTGCTGTCC GCAAAGCCAG AGCAAACAAA 
AAGGCTGGTA GCCCCAAGCA GAAGTTTGTG CATTTTGGGC CAGTAACTGA GATTGATCAA 
CAGAAATGGG ACAAGCTCGT TATTGGCAGA GCTTCATCCA AAAATGGAAC AGAAGAGGAA 
AGCAAGAAAG ACAGTGTGGA GAATCACTCT GCTGTTGAAA GCTCTCTTTC AACCACTGTT 
GGCAGTCTCG GGTTCGCTGA ACACAGCGAT GAAGTATCTA AGCCACAGAA TGAATCCAGT 
CGCATCGTTA GTGAGGTAGC CACGTCAGGT ACAGATGACT CTTGCAGGAG AATGCTATCA 
CCTGTCCCGG CTTTTGGCAT CGTCTCTGAG AAACAAACCC TGTGCAGTTT GAACAAACGG 
CAAGAAGGCA CCGAGGAAGA CTGCGTCGGG CATTTACCAG ACATTGAGAG AGATGATATG 
TTGGTTAGAA GGATGGGAAC TTTTCAGAGG CGTACCACTA GCCCTGTTCC CATACATTGT 
CCTCCCCTGT CAGTAGCTCC TCATCTGCTG CAGCAGCGGC AAGGGGAGGC AGTAGCTTGT 
GAGAAGGATT TGAAGACTCC ATGGGAAGCA GAAAGTACTG TCACACGAGA AGAGAGCCAA 
GAACAGCCTG AAATCCCTCA GCAGCAGAGC GGTGGAACAG AAAACGAAGT GAAATTTCCA 
GACCCGCAAA GAGATGACAT GTTGGCCAGA AGAACTGGAG CTTTCCTAAA ACAGCCTGGG 
CCCAGTGTTA AGCAGTTCCT TCCCATGCCA TTTTCAAAGC AGCAGAATAA GCAGGGCACA 
GCTAGAGATC TTGAGAAGAA AAATACGAGG CCTGAAAAGT CTCCACAAGT CTGCATTAAT 
AGCTCTGGAA AACCTGTAGT ACAACCGCCG CAAGAACAGC AGCAAGGGCA GGAAAAATGC 
AATCTCCCTC CTGCAGGACA AGATGAGACT ATTTGTACTG CCCAAGTGGG TGAAGGTAGC 
CTTAATGAAG TCCACCTTGG TCAAATGTCT AAGCTTCAGG AGGTTCCTGA GGAAAACAAA 
ACGGAAACAT TGTCATCCAA GGATGAGCAT CTGCAAACAT TCGGCCCCAG AACTGCTGTG 
TCTGATGACG CAGAGAGTAT GAGCATGTTT GACATGAGAT GTGAAGAAGA AGCTGTGACT 
CAGCCTCATA GCAAAGCTCG CCATGAGAAA CTGCAGAACG TACACAACCA GCTGAAAGAG 
GATGAGACCA GATGGCAAGA TGACCTGGCT CGGTGGAAGA GTCGTCGAAG GAGCGCTTCA 
CAGGATTTAA TAAAAAAAGA AGCTGAAAGG AAGAAAATGG AAAGGTTATT GTCTGGAGAT 
GGAACGAATG AGCGCAGAAA AAGCATCAAA ACCTACCGAG AAATTGTGGA AGAGAAAGAG 
AGAAGAGAGC GGGAGCTTCA TGAGGCATAC AAGAATGCAA AGTCACAAGA GGAGGCTGAA 
AGCATCCTCC AGCAGTACAT CGAAAGATTC ACTATCAGTG AAGCTGTCCT TGAGCGTTTG 
CAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAGTGGAGC CAAATTCCCC ACTGAAAGAC 
CCAAATCCTC TGAGATATTT AAGGCAACAG TCACTGCCTG CTCCAAAATT CACTGCCACC 
GTTGAAGCAA CTATTGTTCC CACCACTGAA ATAGAGCCCA GTGGTTCGGC GGGCCGCACA 
TCTCCCAGCA AAAGCATTGT CTCCAAGGCT GTGCCTATGC TGACACCCAA GCCTTACTCA 
CAACCCAAAA ATACACAGGA AGTTTTAAAG AACTTTAAGG TAGATGGAAA AGTCAGCATG 
AATGGAGAAA ATTTCAACGG TGTGGAAGAG AAGGATAAAG AGTGTACAAC AGTAATACTT 
ACTCCTTCGC CAAGCAGATC TCTGAAATTT GACGGAGTAG CCAGAGGGGA CAGGCCCCCG 
GTAGAGTTGA AGAAGGACCC CACAAGTGTT GAGCTCAGTT TACAGAGGCC TGCCACTCAG 
AGTGTGCACA AAGAGCCAAC AGCCTCACTG GTGGAAGCAG ATAGAGCTGC CAGCAAACAG 
GTCGAGGCTA GCCCTGGAAG TGCAGGACCT GTGAGCCCGG TGTGCCCGTG CCCAGGTTCA 
GATCAAAAGC AATTCAAGTC AACTGCAGCT TGTGCCAGCC CTGTTGTGAA GAGATTAGAA 
TTTCTTCCCA GTCCGTTCCC TTCAGAGGAA GCTGCTTCAA GTGAGAAAGA TGTGAAGAAA 
CCTGAAATGG AGCAGCAGAA GGAAGCAGAG CTTCCTGCTG CACCGGAGGT GATGAAACCA 
AAAGCCCTGG AACAAGAGGG GAGTGTAGTA TTGCCATTTT TAAAGAAACT ACCCGAGACC 
AGCCAAGTTA CTCTGCAGAA TTCTGGTCTA CAAGCTGATT CTGATAGTGG TGACAAATCA 
AATCGTTTCA ATTTCTGGTC CTGGGATCCA GAAGAAGAGC GAAAGCGTCA GGAAAGGTGG 
CAGCATGAGC AAGAACGCTT GCTTCAGGAG AAGTATCAGA AGGAGCAGGA CAAGCTGAAA 
GAGGAATGGG AAAAGGCCCA GAGAGAAGTT GAAGAGGAGG AGCGTAGATA CTATGAGGAG 
GAACGTAAGA TAATTGAGGA TACTGTAGTT CCATTCATTG TTTCTTCAAA CTCTGCTGAG 
CTCCTCTCCT CCTCTTCCTC TACCACTGAA GGAAACAAGA CAGCGAACAC AACTGATTCA 
AACAGCTCTC CAGAGGAAGT CAAACAAAAA GAAGTTACAA GGCAGAAAAA GTTGCTCTGG 
GAGCAGGACA GTATACCATC TCCGAAAAGC AAGGAAAATG AACTCTGGCA AGAAAAGAGG 
AGTGGAAATA AAGTGCTCTC AGGACACCCG GAGATGGGTA TCTTGAAAGG AGGTGAACAG 
GAGCACCAGG TGCCAGAGAT GGAGCACAGC TCGCCTGCCA GGCTGAATCT GCCGTTGACT 
CAGGACGTCT CCTGGAATCA GCAGTTGCTG ACAAAGCAGT CCCCACATCG TGCAGAGGAC 
ATTCGGCAGA GACCATTCCT GAGCCAGAGT GCGGGACAAC AGCCGAGCTC CGCAGGAGAA 
ACGAGGAGGG CAGGCTATCA TGAGAACTTC CAATCTGGGC CATCATCTCC CTGCTCTCCT 
GCTGCTCCGA GTCAGTCACC CAACAGGTCT GTCAGTGGAA AGAAGCTTTG TTCTACCTGT 
GGGCTTCCTC TTGGGAAAGG AGCTGCCATG ATTATTGAAA CGCTTGGTCT TTATTTCCAT 
ATTCAGTGCT TCAGGTGTGG CATTTGCAAG GGACAGCTGG GAGACGCAGC AAGCGGGACA 
GATGTCAGGA TTAGAAATGG TCTTCTTAAC TGCACCGATT GTTATATCCG ATCCAGAACT 
GCTGGACAGC CAACTACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019327846.1
CDS	241..5004
Translation	MRKETEDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSETDLPHRKMPDVKKDDMLARRTSHGEPKSAVPFNQYLPNKSNQTFYVPAPLRKKKAEREEYRKSWSTATSPLGDRPFSKPHPETIEEEQNETSGLCEKEKVGSLAPEASSLKNQVGLNQNEGNSIDQSPSCSAKETSAPKGKDAEEIKKLRRLEQAGIKVMSAAQRYGSKKPTCEDNGSTPDIILRRDNPFMTHYQGKDSGSEDEAASRVPDLEKDDFATRRARMNQPKHIVPFNPFLIGPYTSKDKGKLEDIKKPLQMEKQEHARRRRVHMASTRQIVTSKENPLLQTEKTESEEEQQVKVPDTESDDLAKQKSQSTLPQLREDAIFVNASITKADLETWKRLKLSGEPSEEDFKCPSDTLPPAEITVKAAAHDDFAVRKARANKKAGSPKQKFVHFGPVTEIDQQKWDKLVIGRASSKNGTEEESKKDSVENHSAVESSLSTTVGSLGFAEHSDEVSKPQNESSRIVSEVATSGTDDSCRRMLSPVPAFGIVSEKQTLCSLNKRQEGTEEDCVGHLPDIERDDMLVRRMGTFQRRTTSPVPIHCPPLSVAPHLLQQRQGEAVACEKDLKTPWEAESTVTREESQEQPEIPQQQSGGTENEVKFPDPQRDDMLARRTGAFLKQPGPSVKQFLPMPFSKQQNKQGTARDLEKKNTRPEKSPQVCINSSGKPVVQPPQEQQQGQEKCNLPPAGQDETICTAQVGEGSLNEVHLGQMSKLQEVPEENKTETLSSKDEHLQTFGPRTAVSDDAESMSMFDMRCEEEAVTQPHSKARHEKLQNVHNQLKEDETRWQDDLARWKSRRRSASQDLIKKEAERKKMERLLSGDGTNERRKSIKTYREIVEEKERRERELHEAYKNAKSQEEAESILQQYIERFTISEAVLERLQMPKILERSHSVEPNSPLKDPNPLRYLRQQSLPAPKFTATVEATIVPTTEIEPSGSAGRTSPSKSIVSKAVPMLTPKPYSQPKNTQEVLKNFKVDGKVSMNGENFNGVEEKDKECTTVILTPSPSRSLKFDGVARGDRPPVELKKDPTSVELSLQRPATQSVHKEPTASLVEADRAASKQVEASPGSAGPVSPVCPCPGSDQKQFKSTAACASPVVKRLEFLPSPFPSEEAASSEKDVKKPEMEQQKEAELPAAPEVMKPKALEQEGSVVLPFLKKLPETSQVTLQNSGLQADSDSGDKSNRFNFWSWDPEEERKRQERWQHEQERLLQEKYQKEQDKLKEEWEKAQREVEEEERRYYEEERKIIEDTVVPFIVSSNSAELLSSSSSTTEGNKTANTTDSNSSPEEVKQKEVTRQKKLLWEQDSIPSPKSKENELWQEKRSGNKVLSGHPEMGILKGGEQEHQVPEMEHSSPARLNLPLTQDVSWNQQLLTKQSPHRAEDIRQRPFLSQSAGQQPSSAGETRRAGYHENFQSGPSSPCSPAAPSQSPNRSVSGKKLCSTCGLPLGKGAAMIIETLGLYFHIQCFRCGICKGQLGDAASGTDVRIRNGLLNCTDCYIRSRTAGQPTTL

Target ORF information:

RefSeq Version	XM_019472301.1
Organism	Aptenodytes forsteri(emperor penguin)
Definition	Aptenodytes forsteri LIM and calponin homology domains 1 (LIMCH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019472301.1

ORF Insert Sequence:

ATGAGAAAGG AAACGGAGGA TATTGAGAGC CCAAAGCGCA GCATTCGTGA CAGCGGCTAT 
ATAGACTGCT GGGACTCTGA ACGCAGCGAT TCCCTCTCCC CGCCTCGCCA CGGCAGAGAT 
GATTCCTTTG ACAGTCTGGA CTCCTTTGGC TCCCGCTCGC AGCAGACACC GTCTCCAGAT 
GTAGTGCTCA GAGGGAGCAG CGATGGGAGA GGAAGTGACT CGGAAACTGA CCTGCCACAC 
AGAAAGATGC CAGATGTGAA AAAAGATGAT ATGTTGGCGA GGCGGACCTC ACATGGTGAA 
CCTAAATCAG CTGTGCCTTT TAACCAGTAC CTCCCTAACA AGAGTAATCA GACTTTCTAT 
GTACCTGCTC CACTTCGGAA AAAGAAAGCT GAACGAGAAG AGTACCGAAA GAGCTGGAGC 
ACGGCGACTT CACCACTAGG AGACAGACCT TTCAGCAAAC CCCACCCTGA AACCATAGAA 
GAAGAACAGA ATGAAACATC AGGACTGTGT GAAAAGGAGA AAGTGGGCTC CCTGGCTCCT 
GAGGCAAGCT CCCTTAAAAA TCAAGTAGGT TTGAATCAAA ACGAAGGAAA CTCCATAGAC 
CAGTCTCCTT CATGTAGTGC AAAAGAAACA TCAGCACCCA AGGGGAAAGA TGCAGAAGAG 
ATCAAAAAGT TAAGAAGACT CGAACAAGCT GGCATTAAGG TCATGTCAGC AGCCCAGCGC 
TATGGCAGTA AAAAGCCAAC ATGTGAAGAC AACGGAAGCA CCCCAGACAT CATCCTTCGC 
AGGGACAACC CCTTCATGAC ACATTACCAA GGGAAGGATT CTGGTTCAGA AGATGAAGCA 
GCCAGCAGAG TTCCTGATTT AGAAAAGGAT GACTTTGCCA CCCGGAGAGC CAGAATGAAT 
CAACCCAAAC ACATAGTTCC GTTTAACCCC TTTTTAATTG GACCCTATAC AAGTAAAGAT 
AAAGGTAAAC TGGAAGACAT TAAAAAGCCA TTGCAGATGG AAAAGCAGGA ACATGCAAGA 
AGAAGACGGG TGCATATGGC ATCAACAAGG CAAATAGTGA CAAGCAAAGA GAACCCACTT 
CTGCAAACTG AGAAGACTGA GTCAGAGGAG GAACAGCAGG TGAAAGTGCC AGATACTGAG 
AGCGATGATT TGGCTAAACA GAAATCTCAA AGTACCCTTC CTCAGCTACG AGAAGATGCG 
ATTTTTGTGA ATGCCTCTAT AACAAAAGCA GATCTGGAGA CCTGGAAAAG ATTGAAACTG 
TCAGGGGAGC CCAGTGAAGA AGATTTCAAA TGTCCCAGTG ACACTCTGCC ACCAGCTGAA 
ATCACGGTGA AAGCAGCGGC CCACGATGAT TTTGCTGTCC GCAAAGCCAG AGCAAACAAA 
AAGGCTGGTA GCCCCAAGCA GAAGTTTGTG CATTTTGGGC CAGTAACTGA GATTGATCAA 
CAGAAATGGG ACAAGCTCGT TATTGGCAGA GCTTCATCCA AAAATGGAAC AGAAGAGGAA 
AGCAAGAAAG ACAGTGTGGA GAATCACTCT GCTGTTGAAA GCTCTCTTTC AACCACTGTT 
GGCAGTCTCG GGTTCGCTGA ACACAGCGAT GAAGTATCTA AGCCACAGAA TGAATCCAGT 
CGCATCGTTA GTGAGGTAGC CACGTCAGGT ACAGATGACT CTTGCAGGAG AATGCTATCA 
CCTGTCCCGG CTTTTGGCAT CGTCTCTGAG AAACAAACCC TGTGCAGTTT GAACAAACGG 
CAAGAAGGCA CCGAGGAAGA CTGCGTCGGG CATTTACCAG ACATTGAGAG AGATGATATG 
TTGGTTAGAA GGATGGGAAC TTTTCAGAGG CGTACCACTA GCCCTGTTCC CATACATTGT 
CCTCCCCTGT CAGTAGCTCC TCATCTGCTG CAGCAGCGGC AAGGGGAGGC AGTAGCTTGT 
GAGAAGGATT TGAAGACTCC ATGGGAAGCA GAAAGTACTG TCACACGAGA AGAGAGCCAA 
GAACAGCCTG AAATCCCTCA GCAGCAGAGC GGTGGAACAG AAAACGAAGT GAAATTTCCA 
GACCCGCAAA GAGATGACAT GTTGGCCAGA AGAACTGGAG CTTTCCTAAA ACAGCCTGGG 
CCCAGTGTTA AGCAGTTCCT TCCCATGCCA TTTTCAAAGC AGCAGAATAA GCAGGGCACA 
GCTAGAGATC TTGAGAAGAA AAATACGAGG CCTGAAAAGT CTCCACAAGT CTGCATTAAT 
AGCTCTGGAA AACCTGTAGT ACAACCGCCG CAAGAACAGC AGCAAGGGCA GGAAAAATGC 
AATCTCCCTC CTGCAGGACA AGATGAGACT ATTTGTACTG CCCAAGTGGG TGAAGGTAGC 
CTTAATGAAG TCCACCTTGG TCAAATGTCT AAGCTTCAGG AGGTTCCTGA GGAAAACAAA 
ACGGAAACAT TGTCATCCAA GGATGAGCAT CTGCAAACAT TCGGCCCCAG AACTGCTGTG 
TCTGATGACG CAGAGAGTAT GAGCATGTTT GACATGAGAT GTGAAGAAGA AGCTGTGACT 
CAGCCTCATA GCAAAGCTCG CCATGAGAAA CTGCAGAACG TACACAACCA GCTGAAAGAG 
GATGAGACCA GATGGCAAGA TGACCTGGCT CGGTGGAAGA GTCGTCGAAG GAGCGCTTCA 
CAGGATTTAA TAAAAAAAGA AGCTGAAAGG AAGAAAATGG AAAGGTTATT GTCTGGAGAT 
GGAACGAATG AGCGCAGAAA AAGCATCAAA ACCTACCGAG AAATTGTGGA AGAGAAAGAG 
AGAAGAGAGC GGGAGCTTCA TGAGGCATAC AAGAATGCAA AGTCACAAGA GGAGGCTGAA 
AGCATCCTCC AGCAGTACAT CGAAAGATTC ACTATCAGTG AAGCTGTCCT TGAGCGTTTG 
CAGATGCCAA AAATTCTGGA AAGAAGCCAT TCAGTGGAGC CAAATTCCCC ACTGAAAGAC 
CCAAATCCTC TGAGATATTT AAGGCAACAG TCACTGCCTG CTCCAAAATT CACTGCCACC 
GTTGAAGCAA CTATTGTTCC CACCACTGAA ATAGAGCCCA GTGGTTCGGC GGGCCGCACA 
TCTCCCAGCA AAAGCATTGT CTCCAAGGCT GTGCCTATGC TGACACCCAA GCCTTACTCA 
CAACCCAAAA ATACACAGGA AGTTTTAAAG AACTTTAAGG TAGATGGAAA AGTCAGCATG 
AATGGAGAAA ATTTCAACGG TGTGGAAGAG AAGGATAAAG AGTGTACAAC AGTAATACTT 
ACTCCTTCGC CAAGCAGATC TCTGAAATTT GACGGAGTAG CCAGAGGGGA CAGGCCCCCG 
GTAGAGTTGA AGAAGGACCC CACAAGTGTT GAGCTCAGTT TACAGAGGCC TGCCACTCAG 
AGTGTGCACA AAGAGCCAAC AGCCTCACTG GTGGAAGCAG ATAGAGCTGC CAGCAAACAG 
GTCGAGGCTA GCCCTGGAAG TGCAGGACCT GTGAGCCCGG TGTGCCCGTG CCCAGGTTCA 
GATCAAAAGC AATTCAAGTC AACTGCAGCT TGTGCCAGCC CTGTTGTGAA GAGATTAGAA 
TTTCTTCCCA GTCCGTTCCC TTCAGAGGAA GCTGCTTCAA GTGAGAAAGA TGTGAAGAAA 
CCTGAAATGG AGCAGCAGAA GGAAGCAGAG CTTCCTGCTG CACCGGAGGT GATGAAACCA 
AAAGCCCTGG AACAAGAGGG GAGTGTAGTA TTGCCATTTT TAAAGAAACT ACCCGAGACC 
AGCCAAGTTA CTCTGCAGAA TTCTGGTCTA CAAGCTGATT CTGATAGTGG TGACAAATCA 
AATCGTTTCA ATTTCTGGTC CTGGGATCCA GAAGAAGAGC GAAAGCGTCA GGAAAGGTGG 
CAGCATGAGC AAGAACGCTT GCTTCAGGAG AAGTATCAGA AGGAGCAGGA CAAGCTGAAA 
GAGGAATGGG AAAAGGCCCA GAGAGAAGTT GAAGAGGAGG AGCGTAGATA CTATGAGGAG 
GAACGTAAGA TAATTGAGGA TACTGTAGTT CCATTCATTG TTTCTTCAAA CTCTGCTGAG 
CTCCTCTCCT CCTCTTCCTC TACCACTGAA GGAAACAAGA CAGCGAACAC AACTGATTCA 
AACAGCTCTC CAGAGGAAGT CAAACAAAAA GAAGTTACAA GGCAGAAAAA GTTGCTCTGG 
GAGCAGGACA GTATACCATC TCCGAAAAGC AAGGAAAATG AACTCTGGCA AGAAAAGAGG 
AGTGGAAATA AAGTGCTCTC AGGACACCCG GAGATGGGTA TCTTGAAAGG AGGTGAACAG 
GAGCACCAGG TGCCAGAGAT GGAGCACAGC TCGCCTGCCA GGCTGAATCT GCCGTTGACT 
CAGGACGTCT CCTGGAATCA GCAGTTGCTG ACAAAGCAGT CCCCACATCG TGCAGAGGAC 
ATTCGGCAGA GACCATTCCT GAGCCAGAGT GCGGGACAAC AGCCGAGCTC CGCAGGAGAA 
ACGAGGAGGG CAGGCTATCA TGAGAACTTC CAATCTGGGC CATCATCTCC CTGCTCTCCT 
GCTGCTCCGA GTCAGTCACC CAACAGGTCT GTCAGTGGAA AGAAGCTTTG TTCTACCTGT 
GGGCTTCCTC TTGGGAAAGG AGCTGCCATG ATTATTGAAA CGCTTGGTCT TTATTTCCAT 
ATTCAGTGCT TCAGGTGTGG CATTTGCAAG GGACAGCTGG GAGACGCAGC AAGCGGGACA 
GATGTCAGGA TTAGAAATGG TCTTCTTAAC TGCACCGATT GTTATATCCG ATCCAGAACT 
GCTGGACAGC CAACTACATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_009280130.2 ) cDNA ORF clone, Aptenodytes forsteri(emperor penguin) -> XP_009278405.1 Aptenodytes forsteri LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA.

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CloneID	OAp53061
Clone ID Related Accession (Same CDS sequence)	XM_009280130.2
Accession Version	XM_009280130.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Aptenodytes forsteri(emperor penguin)
Product	LIM and calponin homology domains-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008795334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2016 this sequence version replaced XM_009280130.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aptenodytes forsteri Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAGG AAACGGAGGA TATTGAGAGC CCAAAGCGCA GCATTCGTGA CAGCGGCTAT 
ATAGACTGCT GGGACTCTGA ACGCAGCGAT TCCCTCTCCC CGCCTCGCCA CGGCAGAGAT 
GATTCCTTTG ACAGTCTGGA CTCCTTTGGC TCCCGCTCGC AGCAGACACC GTCTCCAGAT 
GTAGTGCTCA GAGGGAGCAG CGATGGGAGA GGAAGTGACT CGGAAACTGA CCTGCCACAC 
AGAAAGATGC CAGATGTGAA AAAAGATGAT ATGTTGGCGA GGCGGACCTC ACATGGTGAA 
CCTAAATCAG CTGTGCCTTT TAACCAGTAC CTCCCTAACA AGAGTAATCA GACTTTCTAT 
GTACCTGCTC CACTTCGGAA AAAGAAAGCT GAACGAGAAG AGTACCGAAA GAGCTGGAGC 
ACGGCGACTT CACCACTAGG AGACAGACCT TTCAGCAAAC CCCACCCTGA AACCATAGAA 
GAAGAACAGA ATGAAACATC AGGACTGTGT GAAAAGGAGA AAGTGGGCTC CCTGGCTCCT 
GAGGCAAGCT CCCTTAAAAA TCAAGTAGGT TTGAATCAAA ACGAAGGAAA CTCCATAGAC 
CAGTCTCCTT CATGTAGTGC AAAAGAAACA TCAGCACCCA AGGGGAAAGA TGCAGAAGAG 
ATCAAAAAGT TAAGAAGACT CGAACAAGCT GGCATTAAGG TCATGTCAGC AGCCCAGCGC 
TATGGCAGTA AAAAGCCAAC ATGTGAAGAC AACGGAAGCA CCCCAGACAT CATCCTTCGC 
AGGGACAACC CCTTCATGAC ACATTACCAA GGGAAGGATT CTGGTTCAGA AGATGAAGCA 
GCCAGCAGAG TTCCTGATTT AGAAAAGGAT GACTTTGCCA CCCGGAGAGC CAGAATGAAT 
CAACCCAAAC ACATAGTTCC GTTTAACCCC TTTTTAATTG GACCCTATAC AAGTAAAGAT 
AAAGGTAAAC TGGAAGACAT TAAAAAGCCA TTGCAGATGG AAAAGCAGGA ACATGCAAGA 
AGAAGACGGG TGCATATGGC ATCAACAAGG CAAATAGTGA CAAGCAAAGA GAACCCACTT 
CTGCAAACTG AGAAGACTGA GTCAGAGGAG GAACAGCAGG TGAAAGTGCC AGATACTGAG 
AGCGATGATT TGGCTAAACA GAAATCTCAA AGTACCCTTC CTCAGCTACG AGAAGATGCG 
ATTTTTGTGA ATGCCTCTAT AACAAAAGCA GATCTGGAGA CCTGGAAAAG ATTGAAACTG 
TCAGGGGAGC CCAGTGAAGA AGATTTCAAA TGTCCCAGTG ACACTCTGCC ACCAGCTGAA 
ATCACGGTGA AAGCAGCGGC CCACGATGAT TTTGCTGTCC GCAAAGCCAG AGCAAACAAA 
AAGGCTGGTA GCCCCAAGCA GAAGTTTGTG CATTTTGGGC CAGTAACTGA GATTGATCAA 
CAGAAATGGG ACAAGCTCGT TATTGGCAGA GCTTCATCCA AAAATGGAAC AGAAGAGGAA 
AGCAAGAAAG ACAGTGTGGA GAATCACTCT GCTGTTGAAA GCTCTCTTTC AACCACTGTT 
GGCAGTCTCG GGTTCGCTGA ACACAGCGAT GAAGTATCTA AGCCACAGAA TGAATCCAGT 
GAGGTAGCCA CGTCAGGTAC AGATGACTCT TGCAGGAGAA TGCTATCACC TGTCCCGGCT 
TTTGGCATCG TCTCTGAGAA ACAAACCCTG TGCAGTTTGA ACAAACGGCA AGAAGGCACC 
GAGGAAGACT GCGTCGGGCA TTTACCAGAC ATTGAGAGAG ATGATATGTT GGTTAGAAGG 
ATGGGAACTT TTCAGAGGCG TACCACTAGC CCTGTTCCCA TACATTGTCC TCCCCTGTCA 
GTAGCTCCTC ATCTGCTGCA GCAGCGGCAA GGGGAGGCAG TAGCTTGTGA GAAGGATTTG 
AAGACTCCAT GGGAAGCAGA AAGTACTGTC ACACGAGAAG AGAGCCAAGA ACAGCCTGAA 
ATCCCTCAGC AGCAGAGCGG TGGAACAGAA AACGAAGTGA AATTTCCAGA CCCGCAAAGA 
GATGACATGT TGGCCAGAAG AACTGGAGCT TTCCTAAAAC AGCCTGGGCC CAGTGTTAAG 
CAGTTCCTTC CCATGCCATT TTCAAAGCAG CAGAATAAGC AGGGCACAGC TAGAGATCTT 
GAGAAGAAAA ATACGAGGCC TGAAAAGTCT CCACAAGTCT GCATTAATAG CTCTGGAAAA 
CCTGTAGTAC AACCGCCGCA AGAACAGCAG CAAGGGCAGG AAAAATGCAA TCTCCCTCCT 
GCAGGACAAG ATGAGACTAT TTGTACTGCC CAAGTGGGTG AAGGTAGCCT TAATGAAGTC 
CACCTTGGTC AAATGTCTAA GCTTCAGGAG GTTCCTGAGG AAAACAAAAC GGAAACATTG 
TCATCCAAGG ATGAGCATCT GCAAACATTC GGCCCCAGAA CTGCTGTGTC TGATGACGCA 
GAGAGTATGA GCATGTTTGA CATGAGATGT GAAGAAGAAG CTGTGACTCA GCCTCATAGC 
AAAGCTCGCC ATGAGAAACT GCAGAACGTA CACAACCAGC TGAAAGAGGA TGAGACCAGA 
TGGCAAGATG ACCTGGCTCG GTGGAAGAGT CGTCGAAGGA GCGCTTCACA GGATTTAATA 
AAAAAAGAAG CTGAAAGGAA GAAAATGGAA AGGTTATTGT CTGGAGATGG AACGAATGAG 
CGCAGAAAAA GCATCAAAAC CTACCGAGAA ATTGTGGAAG AGAAAGAGAG AAGAGAGCGG 
GAGCTTCATG AGGCATACAA GAATGCAAAG TCACAAGAGG AGGCTGAAAG CATCCTCCAG 
CAGTACATCG AAAGATTCAC TATCAGTGAA GCTGTCCTTG AGCGTTTGCA GATGCCAAAA 
ATTCTGGAAA GAAGCCATTC AGTGGAGCCA AATTCCCCAC TGAAAGACCC AAATCCTCTG 
AGATATTTAA GGCAACAGTC ACTGCCTGCT CCAAAATTCA CTGCCACCGT TGAAGCAACT 
ATTGTTCCCA CCACTGAAAT AGAGCCCAGT GGTTCGGCGG GCCGCACATC TCCCAGCAAA 
AGCATTGTCT CCAAGGCTGT GCCTATGCTG ACACCCAAGC CTTACTCACA ACCCAAAAAT 
ACACAGGAAG TTTTAAAGAA CTTTAAGGTA GATGGAAAAG TCAGCATGAA TGGAGAAAAT 
TTCAACGGTG TGGAAGAGAA GGATAAAGAG TGTACAACAG TAATACTTAC TCCTTCGCCA 
AGCAGATCTC TGAAATTTGA CGGAGTAGCC AGAGGGGACA GGCCCCCGGT AGAGTTGAAG 
AAGGACCCCA CAAGTGTTGA GCTCAGTTTA CAGAGGCCTG CCACTCAGAG TGTGCACAAA 
GAGCCAACAG CCTCACTGGT GGAAGCAGAT AGAGCTGCCA GCAAACAGGT CGAGGCTAGC 
CCTGGAAGTG CAGGACCTGT GAGCCCGGTG TGCCCGTGCC CAGGTTCAGA TCAAAAGCAA 
TTCAAGTCAA CTGCAGCTTG TGCCAGCCCT GTTGTGAAGA GATTAGAATT TCTTCCCAGT 
CCGTTCCCTT CAGAGGAAGC TGCTTCAAGT GAGAAAGATG TGAAGAAACC TGAAATGGAG 
CAGCAGAAGG AAGCAGAGCT TCCTGCTGCA CCGGAGGTGA TGAAACCAAA AGCCCTGGAA 
CAAGAGGGGA GTGTAGTATT GCCATTTTTA AAGAAACTAC CCGAGACCAG CCAAGTTACT 
CTGCAGAATT CTGGTCTACA AGCTGATTCT GATAGTGGTG ACAAATCAAA TCGTTTCAAT 
TTCTGGTCCT GGGATCCAGA AGAAGAGCGA AAGCGTCAGG AAAGGTGGCA GCATGAGCAA 
GAACGCTTGC TTCAGGAGAA GTATCAGAAG GAGCAGGACA AGCTGAAAGA GGAATGGGAA 
AAGGCCCAGA GAGAAGTTGA AGAGGAGGAG CGTAGATACT ATGAGGAGGA ACGTAAGATA 
ATTGAGGATA CTGTAGTTCC ATTCATTGTT TCTTCAAACT CTGCTGAGCT CCTCTCCTCC 
TCTTCCTCTA CCACTGAAGG AAACAAGACA GCGAACACAA CTGATTCAAA CAGCTCTCCA 
GAGGAAGTCA AACAAAAAGA AGTTACAAGG CAGAAAAAGT TGCTCTGGGA GCAGGACAGT 
ATACCATCTC CGAAAAGCAA GGAAAATGAA CTCTGGCAAG AAAAGAGGAG TGGAAATAAA 
GTGCTCTCAG GACACCCGGA GATGGGTATC TTGAAAGGAG GTGAACAGGA GCACCAGGTG 
CCAGAGATGG AGCACAGCTC GCCTGCCAGG CTGAATCTGC CGTTGACTCA GGACGTCTCC 
TGGAATCAGC AGTTGCTGAC AAAGCAGTCC CCACATCGTG CAGAGGACAT TCGGCAGAGA 
CCATTCCTGA GCCAGAGTGC GGGACAACAG CCGAGCTCCG CAGGAGAAAC GAGGAGGGCA 
GGCTATCATG AGAACTTCCA ATCTGGGCCA TCATCTCCCT GCTCTCCTGC TGCTCCGAGT 
CAGTCACCCA ACAGGTACAA AAGGTCTGTC AGTGGAAAGA AGCTTTGTTC TACCTGTGGG 
CTTCCTCTTG GGAAAGGAGC TGCCATGATT ATTGAAACGC TTGGTCTTTA TTTCCATATT 
CAGTGCTTCA GGTGTGGCAT TTGCAAGGGA CAGCTGGGAG ACGCAGCAAG CGGGACAGAT 
GTCAGGATTA GAAATGGTCT TCTTAACTGC ACCGATTGTT ATATCCGATC CAGAACTGCT 
GGACAGCCAA CTACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009278405.1
CDS	382..5142
Translation	MRKETEDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSETDLPHRKMPDVKKDDMLARRTSHGEPKSAVPFNQYLPNKSNQTFYVPAPLRKKKAEREEYRKSWSTATSPLGDRPFSKPHPETIEEEQNETSGLCEKEKVGSLAPEASSLKNQVGLNQNEGNSIDQSPSCSAKETSAPKGKDAEEIKKLRRLEQAGIKVMSAAQRYGSKKPTCEDNGSTPDIILRRDNPFMTHYQGKDSGSEDEAASRVPDLEKDDFATRRARMNQPKHIVPFNPFLIGPYTSKDKGKLEDIKKPLQMEKQEHARRRRVHMASTRQIVTSKENPLLQTEKTESEEEQQVKVPDTESDDLAKQKSQSTLPQLREDAIFVNASITKADLETWKRLKLSGEPSEEDFKCPSDTLPPAEITVKAAAHDDFAVRKARANKKAGSPKQKFVHFGPVTEIDQQKWDKLVIGRASSKNGTEEESKKDSVENHSAVESSLSTTVGSLGFAEHSDEVSKPQNESSEVATSGTDDSCRRMLSPVPAFGIVSEKQTLCSLNKRQEGTEEDCVGHLPDIERDDMLVRRMGTFQRRTTSPVPIHCPPLSVAPHLLQQRQGEAVACEKDLKTPWEAESTVTREESQEQPEIPQQQSGGTENEVKFPDPQRDDMLARRTGAFLKQPGPSVKQFLPMPFSKQQNKQGTARDLEKKNTRPEKSPQVCINSSGKPVVQPPQEQQQGQEKCNLPPAGQDETICTAQVGEGSLNEVHLGQMSKLQEVPEENKTETLSSKDEHLQTFGPRTAVSDDAESMSMFDMRCEEEAVTQPHSKARHEKLQNVHNQLKEDETRWQDDLARWKSRRRSASQDLIKKEAERKKMERLLSGDGTNERRKSIKTYREIVEEKERRERELHEAYKNAKSQEEAESILQQYIERFTISEAVLERLQMPKILERSHSVEPNSPLKDPNPLRYLRQQSLPAPKFTATVEATIVPTTEIEPSGSAGRTSPSKSIVSKAVPMLTPKPYSQPKNTQEVLKNFKVDGKVSMNGENFNGVEEKDKECTTVILTPSPSRSLKFDGVARGDRPPVELKKDPTSVELSLQRPATQSVHKEPTASLVEADRAASKQVEASPGSAGPVSPVCPCPGSDQKQFKSTAACASPVVKRLEFLPSPFPSEEAASSEKDVKKPEMEQQKEAELPAAPEVMKPKALEQEGSVVLPFLKKLPETSQVTLQNSGLQADSDSGDKSNRFNFWSWDPEEERKRQERWQHEQERLLQEKYQKEQDKLKEEWEKAQREVEEEERRYYEEERKIIEDTVVPFIVSSNSAELLSSSSSTTEGNKTANTTDSNSSPEEVKQKEVTRQKKLLWEQDSIPSPKSKENELWQEKRSGNKVLSGHPEMGILKGGEQEHQVPEMEHSSPARLNLPLTQDVSWNQQLLTKQSPHRAEDIRQRPFLSQSAGQQPSSAGETRRAGYHENFQSGPSSPCSPAAPSQSPNRYKRSVSGKKLCSTCGLPLGKGAAMIIETLGLYFHIQCFRCGICKGQLGDAASGTDVRIRNGLLNCTDCYIRSRTAGQPTTL

Target ORF information:

RefSeq Version	XM_009280130.2
Organism	Aptenodytes forsteri(emperor penguin)
Definition	Aptenodytes forsteri LIM and calponin homology domains 1 (LIMCH1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009280130.2

ORF Insert Sequence:

ATGAGAAAGG AAACGGAGGA TATTGAGAGC CCAAAGCGCA GCATTCGTGA CAGCGGCTAT 
ATAGACTGCT GGGACTCTGA ACGCAGCGAT TCCCTCTCCC CGCCTCGCCA CGGCAGAGAT 
GATTCCTTTG ACAGTCTGGA CTCCTTTGGC TCCCGCTCGC AGCAGACACC GTCTCCAGAT 
GTAGTGCTCA GAGGGAGCAG CGATGGGAGA GGAAGTGACT CGGAAACTGA CCTGCCACAC 
AGAAAGATGC CAGATGTGAA AAAAGATGAT ATGTTGGCGA GGCGGACCTC ACATGGTGAA 
CCTAAATCAG CTGTGCCTTT TAACCAGTAC CTCCCTAACA AGAGTAATCA GACTTTCTAT 
GTACCTGCTC CACTTCGGAA AAAGAAAGCT GAACGAGAAG AGTACCGAAA GAGCTGGAGC 
ACGGCGACTT CACCACTAGG AGACAGACCT TTCAGCAAAC CCCACCCTGA AACCATAGAA 
GAAGAACAGA ATGAAACATC AGGACTGTGT GAAAAGGAGA AAGTGGGCTC CCTGGCTCCT 
GAGGCAAGCT CCCTTAAAAA TCAAGTAGGT TTGAATCAAA ACGAAGGAAA CTCCATAGAC 
CAGTCTCCTT CATGTAGTGC AAAAGAAACA TCAGCACCCA AGGGGAAAGA TGCAGAAGAG 
ATCAAAAAGT TAAGAAGACT CGAACAAGCT GGCATTAAGG TCATGTCAGC AGCCCAGCGC 
TATGGCAGTA AAAAGCCAAC ATGTGAAGAC AACGGAAGCA CCCCAGACAT CATCCTTCGC 
AGGGACAACC CCTTCATGAC ACATTACCAA GGGAAGGATT CTGGTTCAGA AGATGAAGCA 
GCCAGCAGAG TTCCTGATTT AGAAAAGGAT GACTTTGCCA CCCGGAGAGC CAGAATGAAT 
CAACCCAAAC ACATAGTTCC GTTTAACCCC TTTTTAATTG GACCCTATAC AAGTAAAGAT 
AAAGGTAAAC TGGAAGACAT TAAAAAGCCA TTGCAGATGG AAAAGCAGGA ACATGCAAGA 
AGAAGACGGG TGCATATGGC ATCAACAAGG CAAATAGTGA CAAGCAAAGA GAACCCACTT 
CTGCAAACTG AGAAGACTGA GTCAGAGGAG GAACAGCAGG TGAAAGTGCC AGATACTGAG 
AGCGATGATT TGGCTAAACA GAAATCTCAA AGTACCCTTC CTCAGCTACG AGAAGATGCG 
ATTTTTGTGA ATGCCTCTAT AACAAAAGCA GATCTGGAGA CCTGGAAAAG ATTGAAACTG 
TCAGGGGAGC CCAGTGAAGA AGATTTCAAA TGTCCCAGTG ACACTCTGCC ACCAGCTGAA 
ATCACGGTGA AAGCAGCGGC CCACGATGAT TTTGCTGTCC GCAAAGCCAG AGCAAACAAA 
AAGGCTGGTA GCCCCAAGCA GAAGTTTGTG CATTTTGGGC CAGTAACTGA GATTGATCAA 
CAGAAATGGG ACAAGCTCGT TATTGGCAGA GCTTCATCCA AAAATGGAAC AGAAGAGGAA 
AGCAAGAAAG ACAGTGTGGA GAATCACTCT GCTGTTGAAA GCTCTCTTTC AACCACTGTT 
GGCAGTCTCG GGTTCGCTGA ACACAGCGAT GAAGTATCTA AGCCACAGAA TGAATCCAGT 
GAGGTAGCCA CGTCAGGTAC AGATGACTCT TGCAGGAGAA TGCTATCACC TGTCCCGGCT 
TTTGGCATCG TCTCTGAGAA ACAAACCCTG TGCAGTTTGA ACAAACGGCA AGAAGGCACC 
GAGGAAGACT GCGTCGGGCA TTTACCAGAC ATTGAGAGAG ATGATATGTT GGTTAGAAGG 
ATGGGAACTT TTCAGAGGCG TACCACTAGC CCTGTTCCCA TACATTGTCC TCCCCTGTCA 
GTAGCTCCTC ATCTGCTGCA GCAGCGGCAA GGGGAGGCAG TAGCTTGTGA GAAGGATTTG 
AAGACTCCAT GGGAAGCAGA AAGTACTGTC ACACGAGAAG AGAGCCAAGA ACAGCCTGAA 
ATCCCTCAGC AGCAGAGCGG TGGAACAGAA AACGAAGTGA AATTTCCAGA CCCGCAAAGA 
GATGACATGT TGGCCAGAAG AACTGGAGCT TTCCTAAAAC AGCCTGGGCC CAGTGTTAAG 
CAGTTCCTTC CCATGCCATT TTCAAAGCAG CAGAATAAGC AGGGCACAGC TAGAGATCTT 
GAGAAGAAAA ATACGAGGCC TGAAAAGTCT CCACAAGTCT GCATTAATAG CTCTGGAAAA 
CCTGTAGTAC AACCGCCGCA AGAACAGCAG CAAGGGCAGG AAAAATGCAA TCTCCCTCCT 
GCAGGACAAG ATGAGACTAT TTGTACTGCC CAAGTGGGTG AAGGTAGCCT TAATGAAGTC 
CACCTTGGTC AAATGTCTAA GCTTCAGGAG GTTCCTGAGG AAAACAAAAC GGAAACATTG 
TCATCCAAGG ATGAGCATCT GCAAACATTC GGCCCCAGAA CTGCTGTGTC TGATGACGCA 
GAGAGTATGA GCATGTTTGA CATGAGATGT GAAGAAGAAG CTGTGACTCA GCCTCATAGC 
AAAGCTCGCC ATGAGAAACT GCAGAACGTA CACAACCAGC TGAAAGAGGA TGAGACCAGA 
TGGCAAGATG ACCTGGCTCG GTGGAAGAGT CGTCGAAGGA GCGCTTCACA GGATTTAATA 
AAAAAAGAAG CTGAAAGGAA GAAAATGGAA AGGTTATTGT CTGGAGATGG AACGAATGAG 
CGCAGAAAAA GCATCAAAAC CTACCGAGAA ATTGTGGAAG AGAAAGAGAG AAGAGAGCGG 
GAGCTTCATG AGGCATACAA GAATGCAAAG TCACAAGAGG AGGCTGAAAG CATCCTCCAG 
CAGTACATCG AAAGATTCAC TATCAGTGAA GCTGTCCTTG AGCGTTTGCA GATGCCAAAA 
ATTCTGGAAA GAAGCCATTC AGTGGAGCCA AATTCCCCAC TGAAAGACCC AAATCCTCTG 
AGATATTTAA GGCAACAGTC ACTGCCTGCT CCAAAATTCA CTGCCACCGT TGAAGCAACT 
ATTGTTCCCA CCACTGAAAT AGAGCCCAGT GGTTCGGCGG GCCGCACATC TCCCAGCAAA 
AGCATTGTCT CCAAGGCTGT GCCTATGCTG ACACCCAAGC CTTACTCACA ACCCAAAAAT 
ACACAGGAAG TTTTAAAGAA CTTTAAGGTA GATGGAAAAG TCAGCATGAA TGGAGAAAAT 
TTCAACGGTG TGGAAGAGAA GGATAAAGAG TGTACAACAG TAATACTTAC TCCTTCGCCA 
AGCAGATCTC TGAAATTTGA CGGAGTAGCC AGAGGGGACA GGCCCCCGGT AGAGTTGAAG 
AAGGACCCCA CAAGTGTTGA GCTCAGTTTA CAGAGGCCTG CCACTCAGAG TGTGCACAAA 
GAGCCAACAG CCTCACTGGT GGAAGCAGAT AGAGCTGCCA GCAAACAGGT CGAGGCTAGC 
CCTGGAAGTG CAGGACCTGT GAGCCCGGTG TGCCCGTGCC CAGGTTCAGA TCAAAAGCAA 
TTCAAGTCAA CTGCAGCTTG TGCCAGCCCT GTTGTGAAGA GATTAGAATT TCTTCCCAGT 
CCGTTCCCTT CAGAGGAAGC TGCTTCAAGT GAGAAAGATG TGAAGAAACC TGAAATGGAG 
CAGCAGAAGG AAGCAGAGCT TCCTGCTGCA CCGGAGGTGA TGAAACCAAA AGCCCTGGAA 
CAAGAGGGGA GTGTAGTATT GCCATTTTTA AAGAAACTAC CCGAGACCAG CCAAGTTACT 
CTGCAGAATT CTGGTCTACA AGCTGATTCT GATAGTGGTG ACAAATCAAA TCGTTTCAAT 
TTCTGGTCCT GGGATCCAGA AGAAGAGCGA AAGCGTCAGG AAAGGTGGCA GCATGAGCAA 
GAACGCTTGC TTCAGGAGAA GTATCAGAAG GAGCAGGACA AGCTGAAAGA GGAATGGGAA 
AAGGCCCAGA GAGAAGTTGA AGAGGAGGAG CGTAGATACT ATGAGGAGGA ACGTAAGATA 
ATTGAGGATA CTGTAGTTCC ATTCATTGTT TCTTCAAACT CTGCTGAGCT CCTCTCCTCC 
TCTTCCTCTA CCACTGAAGG AAACAAGACA GCGAACACAA CTGATTCAAA CAGCTCTCCA 
GAGGAAGTCA AACAAAAAGA AGTTACAAGG CAGAAAAAGT TGCTCTGGGA GCAGGACAGT 
ATACCATCTC CGAAAAGCAA GGAAAATGAA CTCTGGCAAG AAAAGAGGAG TGGAAATAAA 
GTGCTCTCAG GACACCCGGA GATGGGTATC TTGAAAGGAG GTGAACAGGA GCACCAGGTG 
CCAGAGATGG AGCACAGCTC GCCTGCCAGG CTGAATCTGC CGTTGACTCA GGACGTCTCC 
TGGAATCAGC AGTTGCTGAC AAAGCAGTCC CCACATCGTG CAGAGGACAT TCGGCAGAGA 
CCATTCCTGA GCCAGAGTGC GGGACAACAG CCGAGCTCCG CAGGAGAAAC GAGGAGGGCA 
GGCTATCATG AGAACTTCCA ATCTGGGCCA TCATCTCCCT GCTCTCCTGC TGCTCCGAGT 
CAGTCACCCA ACAGGTACAA AAGGTCTGTC AGTGGAAAGA AGCTTTGTTC TACCTGTGGG 
CTTCCTCTTG GGAAAGGAGC TGCCATGATT ATTGAAACGC TTGGTCTTTA TTTCCATATT 
CAGTGCTTCA GGTGTGGCAT TTGCAAGGGA CAGCTGGGAG ACGCAGCAAG CGGGACAGAT 
GTCAGGATTA GAAATGGTCT TCTTAACTGC ACCGATTGTT ATATCCGATC CAGAACTGCT 
GGACAGCCAA CTACATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMCH1 ( XM_019472304.1 ) cDNA ORF clone, Aptenodytes forsteri(emperor penguin) -> XP_019327849.1 Aptenodytes forsteri LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA.

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CloneID	OAp53062
Clone ID Related Accession (Same CDS sequence)	XM_019472304.1
Accession Version	XM_019472304.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Aptenodytes forsteri(emperor penguin)
Product	LIM and calponin homology domains-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008795334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aptenodytes forsteri Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAGG AAACGGAGGA TATTGAGAGC CCAAAGCGCA GCATTCGTGA CAGCGGCTAT 
ATAGACTGCT GGGACTCTGA ACGCAGCGAT TCCCTCTCCC CGCCTCGCCA CGGCAGAGAT 
GATTCCTTTG ACAGTCTGGA CTCCTTTGGC TCCCGCTCGC AGCAGACACC GTCTCCAGAT 
GTAGTGCTCA GAGGGAGCAG CGATGGGAGA GGAAGTGACT CGGAAACTGA CCTGCCACAC 
AGAAAGATGC CAGATGTGAA AAAAGATGAT ATGTTGGCGA GGCGGACCTC ACATGGTGAA 
CCTAAATCAG CTGTGCCTTT TAACCAGTAC CTCCCTAACA AGAGTAATCA GACTTTCTAT 
GTACCTGCTC CACTTCGGAA AAAGAAAGCT GAACGAGAAG AGTACCGAAA GAGCTGGAGC 
ACGGCGACTT CACCACTAGG AGACAGACCT TTCAGCAAAC CCCACCCTGA AACCATAGAA 
GAAGAACAGA ATGAAACATC AGGACTGTGT GAAAAGGAGA AAGTGGGCTC CCTGGCTCCT 
GAGGCAAGCT CCCTTAAAAA TCAAGTAGGT TTGAATCAAA ACGAAGGAAA CTCCATAGAC 
CAGTCTCCTT CATGTAGTGC AAAAGAAACA TCAGCACCCA AGGGGAAAGA TGCAGAAGAG 
ATCAAAAAGT TAAGAAGACT CGAACAAGCT GGCATTAAGG TCATGTCAGC AGCCCAGCGC 
TATGGCAGTA AAAAGCCAAC ATGTGAAGAC AACGGAAGCA CCCCAGACAT CATCCTTCGC 
AGGGACAACC CCTTCATGAC ACATTACCAA GGGAAGGATT CTGGTTCAGA AGATGAAGCA 
GCCAGCAGAG TTCCTGATTT AGAAAAGGAT GACTTTGCCA CCCGGAGAGC CAGAATGAAT 
CAACCCAAAC ACATAGTTCC GTTTAACCCC TTTTTAATTG GACCCTATAC AAGTAAAGAT 
AAAGGTAAAC TGGAAGACAT TAAAAAGCCA TTGCAGATGG AAAAGCAGGA ACATGCAAGA 
AGAAGACGGG TGCATATGGC ATCAACAAGG CAAATAGTGA CAAGCAAAGA GAACCCACTT 
CTGCAAACTG AGAAGACTGA GTCAGAGGAG GAACAGCAGG TGAAAGTGCC AGATACTGAG 
AGCGATGATT TGGCTAAACA GAAATCTCAA AGTACCCTTC CTCAGCTACG AGAAGATGCG 
ATTTTTGTGA ATGCCTCTAT AACAAAAGCA GATCTGGAGA CCTGGAAAAG ATTGAAACTG 
TCAGGGGAGC CCAGTGAAGA AGATTTCAAA TGTCCCAGTG ACACTCTGCC ACCAGCTGAA 
ATCACGGTGA AAGCAGCGGC CCACGATGAT TTTGCTGTCC GCAAAGCCAG AGCAAACAAA 
AAGGCTGGTA GCCCCAAGCA GAAGTTTGTG CATTTTGGGC CAGTAACTGA GATTGATCAA 
CAGAAATGGG ACAAGCTCGT TATTGGCAGA GCTTCATCCA AAAATGGAAC AGAAGAGGAA 
AGCAAGAAAG ACAGTGTGGA GAATCACTCT GCTGTTGAAA GCTCTCTTTC AACCACTGTT 
GGCAGTCTCG GGTTCGCTGA ACACAGCGAT GAAGTATCTA AGCCACAGAA TGAATCCAGT 
GAGGTAGCCA CGTCAGGTAC AGATGACTCT TGCAGGAGAA TGCTATCACC TGTCCCGGCT 
TTTGGCATCG TCTCTGAGAA ACAAACCCTG TGCAGTTTGA ACAAACGGCA AGAAGGCACC 
GAGGAAGACT GCGTCGGGCA TTTACCAGAC ATTGAGAGAG ATGATATGTT GGTTAGAAGG 
ATGGGAACTT TTCAGAGGCG TACCACTAGC CCTGTTCCCA TACATTGTCC TCCCCTGTCA 
GTAGCTCCTC ATCTGCTGCA GCAGCGGCAA GGGGAGGCAG TAGCTTGTGA GAAGGATTTG 
AAGACTCCAT GGGAAGCAGA AAGTACTGTC ACACGAGAAG AGAGCCAAGA ACAGCCTGAA 
ATCCCTCAGC AGCAGAGCGG TGGAACAGAA AACGAAGTGA AATTTCCAGA CCCGCAAAGA 
GATGACATGT TGGCCAGAAG AACTGGAGCT TTCCTAAAAC AGCCTGGGCC CAGTGTTAAG 
CAGTTCCTTC CCATGCCATT TTCAAAGCAG CAGAATAAGC AGGGCACAGC TAGAGATCTT 
GAGAAGAAAA ATACGAGGCC TGAAAAGTCT CCACAAGTCT GCATTAATAG CTCTGGAAAA 
CCTGTAGTAC AACCGCCGCA AGAACAGCAG CAAGGGCAGG AAAAATGCAA TCTCCCTCCT 
GCAGGACAAG ATGAGACTAT TTGTACTGCC CAAGTGGGTG AAGGTAGCCT TAATGAAGTC 
CACCTTGGTC AAATGTCTAA GCTTCAGGAG GTTCCTGAGG AAAACAAAAC GGAAACATTG 
TCATCCAAGG ATGAGCATCT GCAAACATTC GGCCCCAGAA CTGCTGTGTC TGATGACGCA 
GAGAGTATGA GCATGTTTGA CATGAGATGT GAAGAAGAAG CTGTGACTCA GCCTCATAGC 
AAAGCTCGCC ATGAGAAACT GCAGAACGTA CACAACCAGC TGAAAGAGGA TGAGACCAGA 
TGGCAAGATG ACCTGGCTCG GTGGAAGAGT CGTCGAAGGA GCGCTTCACA GGATTTAATA 
AAAAAAGAAG CTGAAAGGAA GAAAATGGAA AGGTTATTGT CTGGAGATGG AACGAATGAG 
CGCAGAAAAA GCATCAAAAC CTACCGAGAA ATTGTGGAAG AGAAAGAGAG AAGAGAGCGG 
GAGCTTCATG AGGCATACAA GAATGCAAAG TCACAAGAGG AGGCTGAAAG CATCCTCCAG 
CAGTACATCG AAAGATTCAC TATCAGTGAA GCTGTCCTTG AGCGTTTGCA GATGCCAAAA 
ATTCTGGAAA GAAGCCATTC AGTGGAGCCA AATTCCCCAC TGAAAGACCC AAATCCTCTG 
AGATATTTAA GGCAACAGTC ACTGCCTGCT CCAAAATTCA CTGCCACCGT TGAAGCAACT 
ATTGTTCCCA CCACTGAAAT AGAGCCCAGT GGTTCGGCGG GCCGCACATC TCCCAGCAAA 
AGCATTGTCT CCAAGGCTGT GCCTATGCTG ACACCCAAGC CTTACTCACA ACCCAAAAAT 
ACACAGGAAG TTTTAAAGAA CTTTAAGGTA GATGGAAAAG TCAGCATGAA TGGAGAAAAT 
TTCAACGGTG TGGAAGAGAA GGATAAAGAG TGTACAACAG TAATACTTAC TCCTTCGCCA 
AGCAGATCTC TGAAATTTGA CGGAGTAGCC AGAGGGGACA GGCCCCCGGT AGAGTTGAAG 
AAGGACCCCA CAAGTGTTGA GCTCAGTTTA CAGAGGCCTG CCACTCAGAG TGTGCACAAA 
GAGCCAACAG CCTCACTGGT GGAAGCAGAT AGAGCTGCCA GCAAACAGGT CGAGGCTAGC 
CCTGGAAGTG CAGGACCTGT GAGCCCGGTG TGCCCGTGCC CAGGTTCAGA TCAAAAGCAA 
TTCAAGTCAA CTGCAGCTTG TGCCAGCCCT GTTGTGAAGA GATTAGAATT TCTTCCCAGT 
CCGTTCCCTT CAGAGGAAGC TGCTTCAAGT GAGAAAGATG TGAAGAAACC TGAAATGGAG 
CAGCAGAAGG AAGCAGAGCT TCCTGCTGCA CCGGAGGTGA TGAAACCAAA AGCCCTGGAA 
CAAGAGGGGA GTGTAGTATT GCCATTTTTA AAGAAACTAC CCGAGACCAG CCAAGTTACT 
CTGCAGAATT CTGGTCTACA AGCTGATTCT GATAGTGGTG ACAAATCAAA TCGTTTCAAT 
TTCTGGTCCT GGGATCCAGA AGAAGAGCGA AAGCGTCAGG AAAGGTGGCA GCATGAGCAA 
GAACGCTTGC TTCAGGAGAA GTATCAGAAG GAGCAGGACA AGCTGAAAGA GGAATGGGAA 
AAGGCCCAGA GAGAAGTTGA AGAGGAGGAG CGTAGATACT ATGAGGAGGA ACGTAAGATA 
ATTGAGGATA CTGTAGTTCC ATTCATTGTT TCTTCAAACT CTGCTGAGCT CCTCTCCTCC 
TCTTCCTCTA CCACTGAAGG AAACAAGACA GCGAACACAA CTGATTCAAA CAGCTCTCCA 
GAGGAAGTCA AACAAAAAGA AGTTACAAGG CAGAAAAAGT TGCTCTGGGA GCAGGACAGT 
ATACCATCTC CGAAAAGCAA GGAAAATGAA CTCTGGCAAG AAAAGAGGAG TGGAAATAAA 
GTGCTCTCAG GACACCCGGA GATGGGTATC TTGAAAGGAG GTGAACAGGA GCACCAGGTG 
CCAGAGATGG AGCACAGCTC GCCTGCCAGG CTGAATCTGC CGTTGACTCA GGACGTCTCC 
TGGAATCAGC AGTTGCTGAC AAAGCAGTCC CCACATCGTG CAGAGGACAT TCGGCAGAGA 
CCATTCCTGA GCCAGAGTGC GGGACAACAG CCGAGCTCCG CAGGAGAAAC GAGGAGGGCA 
GGCTATCATG AGAACTTCCA ATCTGGGCCA TCATCTCCCT GCTCTCCTGC TGCTCCGAGT 
CAGTCACCCA ACAGGTCTGT CAGTGGAAAG AAGCTTTGTT CTACCTGTGG GCTTCCTCTT 
GGGAAAGGAG CTGCCATGAT TATTGAAACG CTTGGTCTTT ATTTCCATAT TCAGTGCTTC 
AGGTGTGGCA TTTGCAAGGG ACAGCTGGGA GACGCAGCAA GCGGGACAGA TGTCAGGATT 
AGAAATGGTC TTCTTAACTG CACCGATTGT TATATCCGAT CCAGAACTGC TGGACAGCCA 
ACTACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019327849.1
CDS	382..5133
Translation	MRKETEDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSETDLPHRKMPDVKKDDMLARRTSHGEPKSAVPFNQYLPNKSNQTFYVPAPLRKKKAEREEYRKSWSTATSPLGDRPFSKPHPETIEEEQNETSGLCEKEKVGSLAPEASSLKNQVGLNQNEGNSIDQSPSCSAKETSAPKGKDAEEIKKLRRLEQAGIKVMSAAQRYGSKKPTCEDNGSTPDIILRRDNPFMTHYQGKDSGSEDEAASRVPDLEKDDFATRRARMNQPKHIVPFNPFLIGPYTSKDKGKLEDIKKPLQMEKQEHARRRRVHMASTRQIVTSKENPLLQTEKTESEEEQQVKVPDTESDDLAKQKSQSTLPQLREDAIFVNASITKADLETWKRLKLSGEPSEEDFKCPSDTLPPAEITVKAAAHDDFAVRKARANKKAGSPKQKFVHFGPVTEIDQQKWDKLVIGRASSKNGTEEESKKDSVENHSAVESSLSTTVGSLGFAEHSDEVSKPQNESSEVATSGTDDSCRRMLSPVPAFGIVSEKQTLCSLNKRQEGTEEDCVGHLPDIERDDMLVRRMGTFQRRTTSPVPIHCPPLSVAPHLLQQRQGEAVACEKDLKTPWEAESTVTREESQEQPEIPQQQSGGTENEVKFPDPQRDDMLARRTGAFLKQPGPSVKQFLPMPFSKQQNKQGTARDLEKKNTRPEKSPQVCINSSGKPVVQPPQEQQQGQEKCNLPPAGQDETICTAQVGEGSLNEVHLGQMSKLQEVPEENKTETLSSKDEHLQTFGPRTAVSDDAESMSMFDMRCEEEAVTQPHSKARHEKLQNVHNQLKEDETRWQDDLARWKSRRRSASQDLIKKEAERKKMERLLSGDGTNERRKSIKTYREIVEEKERRERELHEAYKNAKSQEEAESILQQYIERFTISEAVLERLQMPKILERSHSVEPNSPLKDPNPLRYLRQQSLPAPKFTATVEATIVPTTEIEPSGSAGRTSPSKSIVSKAVPMLTPKPYSQPKNTQEVLKNFKVDGKVSMNGENFNGVEEKDKECTTVILTPSPSRSLKFDGVARGDRPPVELKKDPTSVELSLQRPATQSVHKEPTASLVEADRAASKQVEASPGSAGPVSPVCPCPGSDQKQFKSTAACASPVVKRLEFLPSPFPSEEAASSEKDVKKPEMEQQKEAELPAAPEVMKPKALEQEGSVVLPFLKKLPETSQVTLQNSGLQADSDSGDKSNRFNFWSWDPEEERKRQERWQHEQERLLQEKYQKEQDKLKEEWEKAQREVEEEERRYYEEERKIIEDTVVPFIVSSNSAELLSSSSSTTEGNKTANTTDSNSSPEEVKQKEVTRQKKLLWEQDSIPSPKSKENELWQEKRSGNKVLSGHPEMGILKGGEQEHQVPEMEHSSPARLNLPLTQDVSWNQQLLTKQSPHRAEDIRQRPFLSQSAGQQPSSAGETRRAGYHENFQSGPSSPCSPAAPSQSPNRSVSGKKLCSTCGLPLGKGAAMIIETLGLYFHIQCFRCGICKGQLGDAASGTDVRIRNGLLNCTDCYIRSRTAGQPTTL

Target ORF information:

RefSeq Version	XM_019472304.1
Organism	Aptenodytes forsteri(emperor penguin)
Definition	Aptenodytes forsteri LIM and calponin homology domains 1 (LIMCH1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019472304.1

ORF Insert Sequence:

ATGAGAAAGG AAACGGAGGA TATTGAGAGC CCAAAGCGCA GCATTCGTGA CAGCGGCTAT 
ATAGACTGCT GGGACTCTGA ACGCAGCGAT TCCCTCTCCC CGCCTCGCCA CGGCAGAGAT 
GATTCCTTTG ACAGTCTGGA CTCCTTTGGC TCCCGCTCGC AGCAGACACC GTCTCCAGAT 
GTAGTGCTCA GAGGGAGCAG CGATGGGAGA GGAAGTGACT CGGAAACTGA CCTGCCACAC 
AGAAAGATGC CAGATGTGAA AAAAGATGAT ATGTTGGCGA GGCGGACCTC ACATGGTGAA 
CCTAAATCAG CTGTGCCTTT TAACCAGTAC CTCCCTAACA AGAGTAATCA GACTTTCTAT 
GTACCTGCTC CACTTCGGAA AAAGAAAGCT GAACGAGAAG AGTACCGAAA GAGCTGGAGC 
ACGGCGACTT CACCACTAGG AGACAGACCT TTCAGCAAAC CCCACCCTGA AACCATAGAA 
GAAGAACAGA ATGAAACATC AGGACTGTGT GAAAAGGAGA AAGTGGGCTC CCTGGCTCCT 
GAGGCAAGCT CCCTTAAAAA TCAAGTAGGT TTGAATCAAA ACGAAGGAAA CTCCATAGAC 
CAGTCTCCTT CATGTAGTGC AAAAGAAACA TCAGCACCCA AGGGGAAAGA TGCAGAAGAG 
ATCAAAAAGT TAAGAAGACT CGAACAAGCT GGCATTAAGG TCATGTCAGC AGCCCAGCGC 
TATGGCAGTA AAAAGCCAAC ATGTGAAGAC AACGGAAGCA CCCCAGACAT CATCCTTCGC 
AGGGACAACC CCTTCATGAC ACATTACCAA GGGAAGGATT CTGGTTCAGA AGATGAAGCA 
GCCAGCAGAG TTCCTGATTT AGAAAAGGAT GACTTTGCCA CCCGGAGAGC CAGAATGAAT 
CAACCCAAAC ACATAGTTCC GTTTAACCCC TTTTTAATTG GACCCTATAC AAGTAAAGAT 
AAAGGTAAAC TGGAAGACAT TAAAAAGCCA TTGCAGATGG AAAAGCAGGA ACATGCAAGA 
AGAAGACGGG TGCATATGGC ATCAACAAGG CAAATAGTGA CAAGCAAAGA GAACCCACTT 
CTGCAAACTG AGAAGACTGA GTCAGAGGAG GAACAGCAGG TGAAAGTGCC AGATACTGAG 
AGCGATGATT TGGCTAAACA GAAATCTCAA AGTACCCTTC CTCAGCTACG AGAAGATGCG 
ATTTTTGTGA ATGCCTCTAT AACAAAAGCA GATCTGGAGA CCTGGAAAAG ATTGAAACTG 
TCAGGGGAGC CCAGTGAAGA AGATTTCAAA TGTCCCAGTG ACACTCTGCC ACCAGCTGAA 
ATCACGGTGA AAGCAGCGGC CCACGATGAT TTTGCTGTCC GCAAAGCCAG AGCAAACAAA 
AAGGCTGGTA GCCCCAAGCA GAAGTTTGTG CATTTTGGGC CAGTAACTGA GATTGATCAA 
CAGAAATGGG ACAAGCTCGT TATTGGCAGA GCTTCATCCA AAAATGGAAC AGAAGAGGAA 
AGCAAGAAAG ACAGTGTGGA GAATCACTCT GCTGTTGAAA GCTCTCTTTC AACCACTGTT 
GGCAGTCTCG GGTTCGCTGA ACACAGCGAT GAAGTATCTA AGCCACAGAA TGAATCCAGT 
GAGGTAGCCA CGTCAGGTAC AGATGACTCT TGCAGGAGAA TGCTATCACC TGTCCCGGCT 
TTTGGCATCG TCTCTGAGAA ACAAACCCTG TGCAGTTTGA ACAAACGGCA AGAAGGCACC 
GAGGAAGACT GCGTCGGGCA TTTACCAGAC ATTGAGAGAG ATGATATGTT GGTTAGAAGG 
ATGGGAACTT TTCAGAGGCG TACCACTAGC CCTGTTCCCA TACATTGTCC TCCCCTGTCA 
GTAGCTCCTC ATCTGCTGCA GCAGCGGCAA GGGGAGGCAG TAGCTTGTGA GAAGGATTTG 
AAGACTCCAT GGGAAGCAGA AAGTACTGTC ACACGAGAAG AGAGCCAAGA ACAGCCTGAA 
ATCCCTCAGC AGCAGAGCGG TGGAACAGAA AACGAAGTGA AATTTCCAGA CCCGCAAAGA 
GATGACATGT TGGCCAGAAG AACTGGAGCT TTCCTAAAAC AGCCTGGGCC CAGTGTTAAG 
CAGTTCCTTC CCATGCCATT TTCAAAGCAG CAGAATAAGC AGGGCACAGC TAGAGATCTT 
GAGAAGAAAA ATACGAGGCC TGAAAAGTCT CCACAAGTCT GCATTAATAG CTCTGGAAAA 
CCTGTAGTAC AACCGCCGCA AGAACAGCAG CAAGGGCAGG AAAAATGCAA TCTCCCTCCT 
GCAGGACAAG ATGAGACTAT TTGTACTGCC CAAGTGGGTG AAGGTAGCCT TAATGAAGTC 
CACCTTGGTC AAATGTCTAA GCTTCAGGAG GTTCCTGAGG AAAACAAAAC GGAAACATTG 
TCATCCAAGG ATGAGCATCT GCAAACATTC GGCCCCAGAA CTGCTGTGTC TGATGACGCA 
GAGAGTATGA GCATGTTTGA CATGAGATGT GAAGAAGAAG CTGTGACTCA GCCTCATAGC 
AAAGCTCGCC ATGAGAAACT GCAGAACGTA CACAACCAGC TGAAAGAGGA TGAGACCAGA 
TGGCAAGATG ACCTGGCTCG GTGGAAGAGT CGTCGAAGGA GCGCTTCACA GGATTTAATA 
AAAAAAGAAG CTGAAAGGAA GAAAATGGAA AGGTTATTGT CTGGAGATGG AACGAATGAG 
CGCAGAAAAA GCATCAAAAC CTACCGAGAA ATTGTGGAAG AGAAAGAGAG AAGAGAGCGG 
GAGCTTCATG AGGCATACAA GAATGCAAAG TCACAAGAGG AGGCTGAAAG CATCCTCCAG 
CAGTACATCG AAAGATTCAC TATCAGTGAA GCTGTCCTTG AGCGTTTGCA GATGCCAAAA 
ATTCTGGAAA GAAGCCATTC AGTGGAGCCA AATTCCCCAC TGAAAGACCC AAATCCTCTG 
AGATATTTAA GGCAACAGTC ACTGCCTGCT CCAAAATTCA CTGCCACCGT TGAAGCAACT 
ATTGTTCCCA CCACTGAAAT AGAGCCCAGT GGTTCGGCGG GCCGCACATC TCCCAGCAAA 
AGCATTGTCT CCAAGGCTGT GCCTATGCTG ACACCCAAGC CTTACTCACA ACCCAAAAAT 
ACACAGGAAG TTTTAAAGAA CTTTAAGGTA GATGGAAAAG TCAGCATGAA TGGAGAAAAT 
TTCAACGGTG TGGAAGAGAA GGATAAAGAG TGTACAACAG TAATACTTAC TCCTTCGCCA 
AGCAGATCTC TGAAATTTGA CGGAGTAGCC AGAGGGGACA GGCCCCCGGT AGAGTTGAAG 
AAGGACCCCA CAAGTGTTGA GCTCAGTTTA CAGAGGCCTG CCACTCAGAG TGTGCACAAA 
GAGCCAACAG CCTCACTGGT GGAAGCAGAT AGAGCTGCCA GCAAACAGGT CGAGGCTAGC 
CCTGGAAGTG CAGGACCTGT GAGCCCGGTG TGCCCGTGCC CAGGTTCAGA TCAAAAGCAA 
TTCAAGTCAA CTGCAGCTTG TGCCAGCCCT GTTGTGAAGA GATTAGAATT TCTTCCCAGT 
CCGTTCCCTT CAGAGGAAGC TGCTTCAAGT GAGAAAGATG TGAAGAAACC TGAAATGGAG 
CAGCAGAAGG AAGCAGAGCT TCCTGCTGCA CCGGAGGTGA TGAAACCAAA AGCCCTGGAA 
CAAGAGGGGA GTGTAGTATT GCCATTTTTA AAGAAACTAC CCGAGACCAG CCAAGTTACT 
CTGCAGAATT CTGGTCTACA AGCTGATTCT GATAGTGGTG ACAAATCAAA TCGTTTCAAT 
TTCTGGTCCT GGGATCCAGA AGAAGAGCGA AAGCGTCAGG AAAGGTGGCA GCATGAGCAA 
GAACGCTTGC TTCAGGAGAA GTATCAGAAG GAGCAGGACA AGCTGAAAGA GGAATGGGAA 
AAGGCCCAGA GAGAAGTTGA AGAGGAGGAG CGTAGATACT ATGAGGAGGA ACGTAAGATA 
ATTGAGGATA CTGTAGTTCC ATTCATTGTT TCTTCAAACT CTGCTGAGCT CCTCTCCTCC 
TCTTCCTCTA CCACTGAAGG AAACAAGACA GCGAACACAA CTGATTCAAA CAGCTCTCCA 
GAGGAAGTCA AACAAAAAGA AGTTACAAGG CAGAAAAAGT TGCTCTGGGA GCAGGACAGT 
ATACCATCTC CGAAAAGCAA GGAAAATGAA CTCTGGCAAG AAAAGAGGAG TGGAAATAAA 
GTGCTCTCAG GACACCCGGA GATGGGTATC TTGAAAGGAG GTGAACAGGA GCACCAGGTG 
CCAGAGATGG AGCACAGCTC GCCTGCCAGG CTGAATCTGC CGTTGACTCA GGACGTCTCC 
TGGAATCAGC AGTTGCTGAC AAAGCAGTCC CCACATCGTG CAGAGGACAT TCGGCAGAGA 
CCATTCCTGA GCCAGAGTGC GGGACAACAG CCGAGCTCCG CAGGAGAAAC GAGGAGGGCA 
GGCTATCATG AGAACTTCCA ATCTGGGCCA TCATCTCCCT GCTCTCCTGC TGCTCCGAGT 
CAGTCACCCA ACAGGTCTGT CAGTGGAAAG AAGCTTTGTT CTACCTGTGG GCTTCCTCTT 
GGGAAAGGAG CTGCCATGAT TATTGAAACG CTTGGTCTTT ATTTCCATAT TCAGTGCTTC 
AGGTGTGGCA TTTGCAAGGG ACAGCTGGGA GACGCAGCAA GCGGGACAGA TGTCAGGATT 
AGAAATGGTC TTCTTAACTG CACCGATTGT TATATCCGAT CCAGAACTGC TGGACAGCCA 
ACTACATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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オンライン注文

LIMCH1 cDNA ORF clone, Aptenodytes forsteri(emperor penguin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム