LOC106012262 cDNA ORF clone, Aplysia californica(California sea hare)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC106012262
Entrez Gene ID	106012262
Full Name	probable E3 ubiquitin-protein ligase TRIM8
Gene Type	protein-coding
Organism	Aplysia californica(California sea hare)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013084643.1	XP_012940097.1	probable E3 ubiquitin-protein ligase TRIM8

Pathways from BioSystems
Gene Ontology

The following LOC106012262 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC106012262 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAp154344	XM_013084643.1 Latest version!	Aplysia californica probable E3 ubiquitin-protein ligase TRIM8 (LOC106012262), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC106012262 ( XM_013084643.1 ) cDNA ORF clone, Aplysia californica(California sea hare) -> XP_012940097.1 Aplysia californica probable E3 ubiquitin-protein ligase TRIM8 (LOC106012262), mRNA.

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CloneID	OAp154344
Clone ID Related Accession (Same CDS sequence)	XM_013084643.1
Accession Version	XM_013084643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-06
Organism	Aplysia californica(California sea hare)
Product	probable E3 ubiquitin-protein ligase TRIM8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004797577.1) and transcript sequences (GBAV01069656.1 and GBBW01036344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aplysia californica Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 739 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGCCGCGT CGACGAGCGG CGGGTTCACG GAAGAGCTCA TCTGCTCCGT GTGTCTGGAG 
ACCTTCAACA CGCCCGTCCT CCTGCCCTGC GCTCACTCCT TCTGCCGGCA GTGTCTCTTC 
AACGTGCACG AGCGGAGCAG ATCAAAGCCA CAACCTAATG GGGGTCAAAA TAACGGCAAC 
AACGCGATAA ACAGCGCCGG TAATAACAAC AATAACAACA ACAGCAATAA CAACGGGACC 
AATACAGCGC CCGGCACTTC GGAAGCGCAG AGGAGAAATG ACGGCGTTCT GGTGTGCCCT 
CAGTGTCGTC TGGAGATCAA ACTGGGACCC GAGGGAATCG CCGGCCTGCC CAAGAACACG 
TCTCTAGCCA ACATCATCAT GAGCGTGGAG GAAGAGAAGA AAGCCAACAA CATTCTGTGC 
GAGGTTTGCG ACGACGAGCC GGCGAGAGTG GCCTCCAAGA AGTGTTCCGA TTGCTCCGTG 
ACATATTGCG TGACGTGTTT CCGGCAGCTT CATCCAATGA GGGGCGCCTT CAAGTACCAC 
GTGATCAAGG AGGCGAACGC ACCAATCAAC AGATGCGCCT CTCCGGTGTT TGACGTGAGG 
GCGGGGCGGT TCACTCCGTC ACGTGATGGG TATGGGACGG ACGGGGCGGA GTCAGGGGAT 
GACAGTGGTG TCTACAGTCA GGACCAGCTG GAGAGTGTGC GAAGACAGAT CAAATCCAAG 
AGAAAGGAAC TGAGTGGGGT CAACAAGGCT GTGCAGAATC TGCTCAAGAC TACAGAGGAC 
GAGACAGAAG AGCGCATGCG TGAGATCCGA TCGACGTGCA GTCAGATCAA CGAGAGTGTG 
GGCGAGAGGG AGGCGGCCAT GTTGACCACA GTCAGTCAGA AGCGCCATGA GGCAGTCCGC 
CAGTGTCTGG CCCTGTCCAG CGAGGTCAAA GCCCAGACCC TGACCCTTGA CCTTCTGGAG 
GACCAGGTCA ATGACATCAT AGCGGTCACC GCCGTGCACA CTGGCACTGA CGTCACCAAC 
GATGTGGAGG CTCTGAAGGA AAAAGTTTCG GCCATCCACT CTCGCTCCCT CATCATGCGA 
GCCAAACTGA TCCCACGACA ACCCAAGCTG GGCGAGACTG AGCTCACCAA ACACATCGCA 
CGCCTCGACT TTGTCAAAGA CAACCACCTG CTGTGTCCAG TCCTGACAGA GTTTGTGTAC 
CGACCGGTCA GTTCCGGCCC CGCCCTGGTG GAGGTCAAGT GGAGGAGCAT GGAGGACGTG 
GTCAAGTTCA AACTTGCGGC GACCCAGAAG GATCCGCGAG GCAACCAGGT CGAGACGTCA 
GTGGACCTGG CGGGCAGCAC GACCTCGTAC CAGCTGTGTC TGCCGTGCTC GGACACGGAG 
TACCACGTCA GTCTGCACGC CACGGCGCAG GACGACGCAC AACACCCGGT CATTGGCCGC 
ACCATTCGCA CGCTGCCCTA CGTGCACTGC TTCAAGGCCA GGTTCAGCCC CACCACGTGT 
CACAAACGTA TCGCGGTGTC GGCCTCCAGA GACGAGGTGG TCCACAGGAA GTGCAAACTG 
TCGAACCTAC ACAACGCGTG TGCCGCCACC ACCAGCTTCC AGCGCCTGGA ACACATAGAG 
GGCGCCATCG CAGACGAAGC CCTGCCCCTC CTCCCCCACC TTTACTGGGA GGCCATCATC 
CACTTCCGGG TGTTCGGCAA ATTGGGCAAC TCCAAGCTCC TCTGTGACCT TGGCGTGGTG 
AAGCAGGGCT GCGAGGACGG CTCCAGTCTG CTGTGCGACA ACTTCAAGTC TTACTGCTCG 
TACCTCGTGC GCCGACAGAA CAAGGTCACT CTCGAGTTCT GGAACGGTCC GGACCGAGAG 
ATCCTCCCTC GGAGTATCCC CGTCCTCGAC CTGGACAACG ACCAGGAGAA GACGATCAAA 
CTGGCGTTCT ACTTGGATGC GCTCAAGAGG AAGCTGGCCA TCGTGAGTCC TGGCAACAAC 
TCGGTGCTGT GCCAGTTCAA CGTCCGCTTC CCCTCGCTGG TGCCCATGTG CGGGCTCTAC 
TGTTTCGACC AGGTCTACGT CATGGTCAAG TTCACAGAGC CGCAGAAGAT CCCCAACGTT 
CTGAGCAAGC TGATGAAACA AAGCACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012940097.1
CDS	169..2298
Translation	MAASTSGGFTEELICSVCLETFNTPVLLPCAHSFCRQCLFNVHERSRSKPQPNGGQNNGNNAINSAGNNNNNNNSNNNGTNTAPGTSEAQRRNDGVLVCPQCRLEIKLGPEGIAGLPKNTSLANIIMSVEEEKKANNILCEVCDDEPARVASKKCSDCSVTYCVTCFRQLHPMRGAFKYHVIKEANAPINRCASPVFDVRAGRFTPSRDGYGTDGAESGDDSGVYSQDQLESVRRQIKSKRKELSGVNKAVQNLLKTTEDETEERMREIRSTCSQINESVGEREAAMLTTVSQKRHEAVRQCLALSSEVKAQTLTLDLLEDQVNDIIAVTAVHTGTDVTNDVEALKEKVSAIHSRSLIMRAKLIPRQPKLGETELTKHIARLDFVKDNHLLCPVLTEFVYRPVSSGPALVEVKWRSMEDVVKFKLAATQKDPRGNQVETSVDLAGSTTSYQLCLPCSDTEYHVSLHATAQDDAQHPVIGRTIRTLPYVHCFKARFSPTTCHKRIAVSASRDEVVHRKCKLSNLHNACAATTSFQRLEHIEGAIADEALPLLPHLYWEAIIHFRVFGKLGNSKLLCDLGVVKQGCEDGSSLLCDNFKSYCSYLVRRQNKVTLEFWNGPDREILPRSIPVLDLDNDQEKTIKLAFYLDALKRKLAIVSPGNNSVLCQFNVRFPSLVPMCGLYCFDQVYVMVKFTEPQKIPNVLSKLMKQST

Target ORF information:

RefSeq Version	XM_013084643.1
Organism	Aplysia californica(California sea hare)
Definition	Aplysia californica probable E3 ubiquitin-protein ligase TRIM8 (LOC106012262), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013084643.1

ORF Insert Sequence:

ATGGCCGCGT CGACGAGCGG CGGGTTCACG GAAGAGCTCA TCTGCTCCGT GTGTCTGGAG 
ACCTTCAACA CGCCCGTCCT CCTGCCCTGC GCTCACTCCT TCTGCCGGCA GTGTCTCTTC 
AACGTGCACG AGCGGAGCAG ATCAAAGCCA CAACCTAATG GGGGTCAAAA TAACGGCAAC 
AACGCGATAA ACAGCGCCGG TAATAACAAC AATAACAACA ACAGCAATAA CAACGGGACC 
AATACAGCGC CCGGCACTTC GGAAGCGCAG AGGAGAAATG ACGGCGTTCT GGTGTGCCCT 
CAGTGTCGTC TGGAGATCAA ACTGGGACCC GAGGGAATCG CCGGCCTGCC CAAGAACACG 
TCTCTAGCCA ACATCATCAT GAGCGTGGAG GAAGAGAAGA AAGCCAACAA CATTCTGTGC 
GAGGTTTGCG ACGACGAGCC GGCGAGAGTG GCCTCCAAGA AGTGTTCCGA TTGCTCCGTG 
ACATATTGCG TGACGTGTTT CCGGCAGCTT CATCCAATGA GGGGCGCCTT CAAGTACCAC 
GTGATCAAGG AGGCGAACGC ACCAATCAAC AGATGCGCCT CTCCGGTGTT TGACGTGAGG 
GCGGGGCGGT TCACTCCGTC ACGTGATGGG TATGGGACGG ACGGGGCGGA GTCAGGGGAT 
GACAGTGGTG TCTACAGTCA GGACCAGCTG GAGAGTGTGC GAAGACAGAT CAAATCCAAG 
AGAAAGGAAC TGAGTGGGGT CAACAAGGCT GTGCAGAATC TGCTCAAGAC TACAGAGGAC 
GAGACAGAAG AGCGCATGCG TGAGATCCGA TCGACGTGCA GTCAGATCAA CGAGAGTGTG 
GGCGAGAGGG AGGCGGCCAT GTTGACCACA GTCAGTCAGA AGCGCCATGA GGCAGTCCGC 
CAGTGTCTGG CCCTGTCCAG CGAGGTCAAA GCCCAGACCC TGACCCTTGA CCTTCTGGAG 
GACCAGGTCA ATGACATCAT AGCGGTCACC GCCGTGCACA CTGGCACTGA CGTCACCAAC 
GATGTGGAGG CTCTGAAGGA AAAAGTTTCG GCCATCCACT CTCGCTCCCT CATCATGCGA 
GCCAAACTGA TCCCACGACA ACCCAAGCTG GGCGAGACTG AGCTCACCAA ACACATCGCA 
CGCCTCGACT TTGTCAAAGA CAACCACCTG CTGTGTCCAG TCCTGACAGA GTTTGTGTAC 
CGACCGGTCA GTTCCGGCCC CGCCCTGGTG GAGGTCAAGT GGAGGAGCAT GGAGGACGTG 
GTCAAGTTCA AACTTGCGGC GACCCAGAAG GATCCGCGAG GCAACCAGGT CGAGACGTCA 
GTGGACCTGG CGGGCAGCAC GACCTCGTAC CAGCTGTGTC TGCCGTGCTC GGACACGGAG 
TACCACGTCA GTCTGCACGC CACGGCGCAG GACGACGCAC AACACCCGGT CATTGGCCGC 
ACCATTCGCA CGCTGCCCTA CGTGCACTGC TTCAAGGCCA GGTTCAGCCC CACCACGTGT 
CACAAACGTA TCGCGGTGTC GGCCTCCAGA GACGAGGTGG TCCACAGGAA GTGCAAACTG 
TCGAACCTAC ACAACGCGTG TGCCGCCACC ACCAGCTTCC AGCGCCTGGA ACACATAGAG 
GGCGCCATCG CAGACGAAGC CCTGCCCCTC CTCCCCCACC TTTACTGGGA GGCCATCATC 
CACTTCCGGG TGTTCGGCAA ATTGGGCAAC TCCAAGCTCC TCTGTGACCT TGGCGTGGTG 
AAGCAGGGCT GCGAGGACGG CTCCAGTCTG CTGTGCGACA ACTTCAAGTC TTACTGCTCG 
TACCTCGTGC GCCGACAGAA CAAGGTCACT CTCGAGTTCT GGAACGGTCC GGACCGAGAG 
ATCCTCCCTC GGAGTATCCC CGTCCTCGAC CTGGACAACG ACCAGGAGAA GACGATCAAA 
CTGGCGTTCT ACTTGGATGC GCTCAAGAGG AAGCTGGCCA TCGTGAGTCC TGGCAACAAC 
TCGGTGCTGT GCCAGTTCAA CGTCCGCTTC CCCTCGCTGG TGCCCATGTG CGGGCTCTAC 
TGTTTCGACC AGGTCTACGT CATGGTCAAG TTCACAGAGC CGCAGAAGAT CCCCAACGTT 
CTGAGCAAGC TGATGAAACA AAGCACGTAA