LOC101853918 cDNA ORF clone, Aplysia californica(California sea hare)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101853918
Entrez Gene ID	101853918
Full Name	ribosomal RNA small subunit methyltransferase NEP1-like
Gene Type	protein-coding
Organism	Aplysia californica(California sea hare)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005093854.1	XP_005093911.1	ribosomal RNA small subunit methyltransferase NEP1-like

Pathways from BioSystems
Gene Ontology

The following LOC101853918 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101853918 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAp146877	XM_005093854.1 Latest version!	Aplysia californica ribosomal RNA small subunit methyltransferase NEP1-like (LOC101853918), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101853918 ( XM_005093854.1 ) cDNA ORF clone, Aplysia californica(California sea hare) -> XP_005093911.1 Aplysia californica ribosomal RNA small subunit methyltransferase NEP1-like (LOC101853918), mRNA.

Price & Availability↑

CloneID	OAp146877
Clone ID Related Accession (Same CDS sequence)	XM_005093854.1
Accession Version	XM_005093854.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-06
Organism	Aplysia californica(California sea hare)
Product	ribosomal RNA small subunit methyltransferase NEP1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004797341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aplysia californica Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCCC CCGTAGCTAA TGCACATGAT GCGAAGCCTA AAATAATTAA AAGTGTTCCT 
AAAGACACAG ACGAAAAAAG GTTGATCGTT ATTCTTGAGA AGGCTTCTCT GGAAACAGTA 
AAGGTTGGCC AGAAGTATGA GCTGCTGTCT TGTGACCGAC ACAGTAACAT TGGTGCAAGA 
GTCAAGAAAA ATGTTTCCAC CTGCAGACCC GATATCACCC ATCAGTGCCT ATTGATGTTG 
ATGGATTCTC CCCTCAACAG AGCAGGCCTC TTGCAGGTGT ACGTCAGGAC AGAGAGGAAT 
GTTCTTATTG AAGTCAACCC TCAAACAAGA ATACCTCGAA CTTTTGACAG ATTCTGTGGC 
TTGATGGTTC AGCTCTTAGA CAAACTGAGC ATTCGGGCAA GTGATGGACC CCAAAAATTG 
CTCAAAGTCA TCAAAAATCC TGTGATTAGC CACCTCCCTA CTGGATGCCC CAAGTACGCC 
ATGTCATATA GTTCAGAAGA AATAATTGAC GTTAGAGACT TTGTACCCAA AGATGGACCC 
GCAGTCTTTG TCATCGGAGC CATGGCTCAT GGCAGTGTGT CACCGGACTA CACAGAGCAT 
TCCTACTCCA TCAGTAACTA CCCTCTGTCT GCTGCCATCA CCTGCGCCAA GGTGTGCAAC 
GCGTTTGAAG AGATCTGGGG AGTAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005093911.1
CDS	158..847
Translation	MAAPVANAHDAKPKIIKSVPKDTDEKRLIVILEKASLETVKVGQKYELLSCDRHSNIGARVKKNVSTCRPDITHQCLLMLMDSPLNRAGLLQVYVRTERNVLIEVNPQTRIPRTFDRFCGLMVQLLDKLSIRASDGPQKLLKVIKNPVISHLPTGCPKYAMSYSSEEIIDVRDFVPKDGPAVFVIGAMAHGSVSPDYTEHSYSISNYPLSAAITCAKVCNAFEEIWGVK

Target ORF information:

RefSeq Version	XM_005093854.1
Organism	Aplysia californica(California sea hare)
Definition	Aplysia californica ribosomal RNA small subunit methyltransferase NEP1-like (LOC101853918), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005093854.1

ORF Insert Sequence:

ATGGCAGCCC CCGTAGCTAA TGCACATGAT GCGAAGCCTA AAATAATTAA AAGTGTTCCT 
AAAGACACAG ACGAAAAAAG GTTGATCGTT ATTCTTGAGA AGGCTTCTCT GGAAACAGTA 
AAGGTTGGCC AGAAGTATGA GCTGCTGTCT TGTGACCGAC ACAGTAACAT TGGTGCAAGA 
GTCAAGAAAA ATGTTTCCAC CTGCAGACCC GATATCACCC ATCAGTGCCT ATTGATGTTG 
ATGGATTCTC CCCTCAACAG AGCAGGCCTC TTGCAGGTGT ACGTCAGGAC AGAGAGGAAT 
GTTCTTATTG AAGTCAACCC TCAAACAAGA ATACCTCGAA CTTTTGACAG ATTCTGTGGC 
TTGATGGTTC AGCTCTTAGA CAAACTGAGC ATTCGGGCAA GTGATGGACC CCAAAAATTG 
CTCAAAGTCA TCAAAAATCC TGTGATTAGC CACCTCCCTA CTGGATGCCC CAAGTACGCC 
ATGTCATATA GTTCAGAAGA AATAATTGAC GTTAGAGACT TTGTACCCAA AGATGGACCC 
GCAGTCTTTG TCATCGGAGC CATGGCTCAT GGCAGTGTGT CACCGGACTA CACAGAGCAT 
TCCTACTCCA TCAGTAACTA CCCTCTGTCT GCTGCCATCA CCTGCGCCAA GGTGTGCAAC 
GCGTTTGAAG AGATCTGGGG AGTAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.