RNF220 cDNA ORF clone, Antrostomus carolinensis(chuck-will's-widow)

Gene
Clones
gRNAs

Summary

Gene Symbol	RNF220
Entrez Gene ID	104521225
Full Name	ring finger protein 220
Gene Type	protein-coding
Organism	Antrostomus carolinensis(chuck-will's-widow)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010163155.1	XP_010161457.1	E3 ubiquitin-protein ligase RNF220 isoform X1
XM_010163156.1	XP_010161458.1	E3 ubiquitin-protein ligase RNF220 isoform X2

Pathways from BioSystems
Gene Ontology

The following RNF220 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNF220 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAn94273	XM_010163155.1 Latest version!	Antrostomus carolinensis ring finger protein 220 (RNF220), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
OAn94274	XM_010163156.1 Latest version!	Antrostomus carolinensis ring finger protein 220 (RNF220), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNF220 ( XM_010163155.1 ) cDNA ORF clone, Antrostomus carolinensis(chuck-will's-widow) -> XP_010161457.1 Antrostomus carolinensis ring finger protein 220 (RNF220), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAn94273
Clone ID Related Accession (Same CDS sequence)	XM_010163155.1
Accession Version	XM_010163155.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Antrostomus carolinensis(chuck-will's-widow)
Product	E3 ubiquitin-protein ligase RNF220 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010300472.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Antrostomus carolinensis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACGGA GGAAGCAAGA CGAAGGGCAG GTATGTCCCC TCTGCAGCCG ACCTCTGTCA 
GGATCCGAGG AGGAGATGAG TAGGCATGTG GAACAATGCC TTGCAAAGAG AGAAGGTTCA 
TGCATGACTG AGGATGACTC TGTGGACATC GAGAATGAAA ACGGCAACCG CTTTGAAGAG 
TACGAATGGT GCGGGCAGAA GAGGATACGG GCTACTACGC TCCTGGAGGG AGGATTTCGA 
GGTTCTGGGT TTATCATGTG CAGCGGCAAA GAGAATCCAG ACAGCGACGC TGACCTGGAT 
GTGGATGGGG ACGACACACT TGAATACGGG AAACCACAGT ACACAGAGGC AGACGTTATC 
CCTTGCACTG GGGAAGAGCC AGGTGAAGCC AAAGAGAGGG AGGCGCTCCG AGGTGCTGTT 
TTAAATGGTG GCACACCCAG TACTCGAATA ACACCGGAGT TTTCTAAATG GGCCAGCGAT 
GAAATGCCCT CAACGAGTAA TGGTGAAAGC AGTAAACAGG AGACCATGCA GAAGACCTGT 
AAGAACAGTG ACATTGAAAA AATTACAGAA GACTCTGCAG TGACAACATT TGAAGCGTTA 
AAGGCTCGTG TCCGTGAGTT AGAAAGGCAG CTTTCCCGTG GGGACCGCTA TAAATGTCTC 
ATTTGCATGG ACTCCTACAC AATGCCCCTG ACTTCCATTC AGTGCTGGCA TGTGCACTGT 
GAAGAATGCT GGCTGCGGAC TCTGGGTGCC AAGAAGCTCT GTCCCCAGTG TAACACCATC 
ACGTCTCCCG GAGACCTTCG GCGCATCTAC TTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010161457.1
CDS	10..825
Translation	MKRRKQDEGQVCPLCSRPLSGSEEEMSRHVEQCLAKREGSCMTEDDSVDIENENGNRFEEYEWCGQKRIRATTLLEGGFRGSGFIMCSGKENPDSDADLDVDGDDTLEYGKPQYTEADVIPCTGEEPGEAKEREALRGAVLNGGTPSTRITPEFSKWASDEMPSTSNGESSKQETMQKTCKNSDIEKITEDSAVTTFEALKARVRELERQLSRGDRYKCLICMDSYTMPLTSIQCWHVHCEECWLRTLGAKKLCPQCNTITSPGDLRRIYL

Target ORF information:

RefSeq Version	XM_010163155.1
Organism	Antrostomus carolinensis(chuck-will's-widow)
Definition	Antrostomus carolinensis ring finger protein 220 (RNF220), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010163155.1

ORF Insert Sequence:

ATGAAACGGA GGAAGCAAGA CGAAGGGCAG GTATGTCCCC TCTGCAGCCG ACCTCTGTCA 
GGATCCGAGG AGGAGATGAG TAGGCATGTG GAACAATGCC TTGCAAAGAG AGAAGGTTCA 
TGCATGACTG AGGATGACTC TGTGGACATC GAGAATGAAA ACGGCAACCG CTTTGAAGAG 
TACGAATGGT GCGGGCAGAA GAGGATACGG GCTACTACGC TCCTGGAGGG AGGATTTCGA 
GGTTCTGGGT TTATCATGTG CAGCGGCAAA GAGAATCCAG ACAGCGACGC TGACCTGGAT 
GTGGATGGGG ACGACACACT TGAATACGGG AAACCACAGT ACACAGAGGC AGACGTTATC 
CCTTGCACTG GGGAAGAGCC AGGTGAAGCC AAAGAGAGGG AGGCGCTCCG AGGTGCTGTT 
TTAAATGGTG GCACACCCAG TACTCGAATA ACACCGGAGT TTTCTAAATG GGCCAGCGAT 
GAAATGCCCT CAACGAGTAA TGGTGAAAGC AGTAAACAGG AGACCATGCA GAAGACCTGT 
AAGAACAGTG ACATTGAAAA AATTACAGAA GACTCTGCAG TGACAACATT TGAAGCGTTA 
AAGGCTCGTG TCCGTGAGTT AGAAAGGCAG CTTTCCCGTG GGGACCGCTA TAAATGTCTC 
ATTTGCATGG ACTCCTACAC AATGCCCCTG ACTTCCATTC AGTGCTGGCA TGTGCACTGT 
GAAGAATGCT GGCTGCGGAC TCTGGGTGCC AAGAAGCTCT GTCCCCAGTG TAACACCATC 
ACGTCTCCCG GAGACCTTCG GCGCATCTAC TTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF220 ( XM_010163156.1 ) cDNA ORF clone, Antrostomus carolinensis(chuck-will's-widow) -> XP_010161458.1 Antrostomus carolinensis ring finger protein 220 (RNF220), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAn94274
Clone ID Related Accession (Same CDS sequence)	XM_010163156.1
Accession Version	XM_010163156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Antrostomus carolinensis(chuck-will's-widow)
Product	E3 ubiquitin-protein ligase RNF220 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010300472.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Antrostomus carolinensis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACGGA GGAAGCAAGA CGAAGGGCAG AGAGAAGGTT CATGCATGAC TGAGGATGAC 
TCTGTGGACA TCGAGAATGA AAACGGCAAC CGCTTTGAAG AGTACGAATG GTGCGGGCAG 
AAGAGGATAC GGGCTACTAC GCTCCTGGAG GGAGGATTTC GAGGTTCTGG GTTTATCATG 
TGCAGCGGCA AAGAGAATCC AGACAGCGAC GCTGACCTGG ATGTGGATGG GGACGACACA 
CTTGAATACG GGAAACCACA GTACACAGAG GCAGACGTTA TCCCTTGCAC TGGGGAAGAG 
CCAGGTGAAG CCAAAGAGAG GGAGGCGCTC CGAGGTGCTG TTTTAAATGG TGGCACACCC 
AGTACTCGAA TAACACCGGA GTTTTCTAAA TGGGCCAGCG ATGAAATGCC CTCAACGAGT 
AATGGTGAAA GCAGTAAACA GGAGACCATG CAGAAGACCT GTAAGAACAG TGACATTGAA 
AAAATTACAG AAGACTCTGC AGTGACAACA TTTGAAGCGT TAAAGGCTCG TGTCCGTGAG 
TTAGAAAGGC AGCTTTCCCG TGGGGACCGC TATAAATGTC TCATTTGCAT GGACTCCTAC 
ACAATGCCCC TGACTTCCAT TCAGTGCTGG CATGTGCACT GTGAAGAATG CTGGCTGCGG 
ACTCTGGGTG CCAAGAAGCT CTGTCCCCAG TGTAACACCA TCACGTCTCC CGGAGACCTT 
CGGCGCATCT ACTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010161458.1
CDS	10..747
Translation	MKRRKQDEGQREGSCMTEDDSVDIENENGNRFEEYEWCGQKRIRATTLLEGGFRGSGFIMCSGKENPDSDADLDVDGDDTLEYGKPQYTEADVIPCTGEEPGEAKEREALRGAVLNGGTPSTRITPEFSKWASDEMPSTSNGESSKQETMQKTCKNSDIEKITEDSAVTTFEALKARVRELERQLSRGDRYKCLICMDSYTMPLTSIQCWHVHCEECWLRTLGAKKLCPQCNTITSPGDLRRIYL

Target ORF information:

RefSeq Version	XM_010163156.1
Organism	Antrostomus carolinensis(chuck-will's-widow)
Definition	Antrostomus carolinensis ring finger protein 220 (RNF220), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010163156.1

ORF Insert Sequence:

ATGAAACGGA GGAAGCAAGA CGAAGGGCAG AGAGAAGGTT CATGCATGAC TGAGGATGAC 
TCTGTGGACA TCGAGAATGA AAACGGCAAC CGCTTTGAAG AGTACGAATG GTGCGGGCAG 
AAGAGGATAC GGGCTACTAC GCTCCTGGAG GGAGGATTTC GAGGTTCTGG GTTTATCATG 
TGCAGCGGCA AAGAGAATCC AGACAGCGAC GCTGACCTGG ATGTGGATGG GGACGACACA 
CTTGAATACG GGAAACCACA GTACACAGAG GCAGACGTTA TCCCTTGCAC TGGGGAAGAG 
CCAGGTGAAG CCAAAGAGAG GGAGGCGCTC CGAGGTGCTG TTTTAAATGG TGGCACACCC 
AGTACTCGAA TAACACCGGA GTTTTCTAAA TGGGCCAGCG ATGAAATGCC CTCAACGAGT 
AATGGTGAAA GCAGTAAACA GGAGACCATG CAGAAGACCT GTAAGAACAG TGACATTGAA 
AAAATTACAG AAGACTCTGC AGTGACAACA TTTGAAGCGT TAAAGGCTCG TGTCCGTGAG 
TTAGAAAGGC AGCTTTCCCG TGGGGACCGC TATAAATGTC TCATTTGCAT GGACTCCTAC 
ACAATGCCCC TGACTTCCAT TCAGTGCTGG CATGTGCACT GTGAAGAATG CTGGCTGCGG 
ACTCTGGGTG CCAAGAAGCT CTGTCCCCAG TGTAACACCA TCACGTCTCC CGGAGACCTT 
CGGCGCATCT ACTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.