prim1 cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	prim1
Entrez Gene ID	111570404
Full Name	DNA primase subunit 1
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023273151.1	XP_023128919.1	DNA primase small subunit

Pathways from BioSystems
Gene Ontology

The following prim1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prim1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm80132	XM_023273151.1 Latest version!	Amphiprion ocellaris DNA primase subunit 1 (prim1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

prim1 ( XM_023273151.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023128919.1 Amphiprion ocellaris DNA primase subunit 1 (prim1), mRNA.

Price & Availability↑

CloneID	OAm80132
Clone ID Related Accession (Same CDS sequence)	XM_023273151.1
Accession Version	XM_023273151.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	DNA primase small subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019372274.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTCCT CAGACTACGA CCCGGCGTCT CTACCCGACT TATTACCGCT GTACTACCGC 
CGGCTGTTCC CCTTCTCACA GTATTACCGC TGGCTCAACT ATGGAGGAGT GCAGAAGAAC 
TACTTTCAGA ATCGAGAGTT TTCCTTCACG CTCAAAGACG ACATCTACGT CCGCTACCAG 
TCGTTCACCA CCCAGAATGA GCTGGAGAAG GAAATGCAGA AGATGAACCC CTACAAGATT 
GACATCGGAG CAATATACAG TCACAGGCCG AATCAACACA ACACTGTGAA GTCAGGGACC 
TTCCAGGCTC TGGAGAAGGA GCTGGTGTTT GATATTGATA TGACAGATTA TGATGATGTC 
CGAAGCTGCT GCAGCGCTGC AGACATTTGC TGCAAGTGCT GGACTTTGAT GACCATCGCT 
ATTCGTATCC TGGATCGAGC TCTTCGAGAG GACTTTGGTT TCCAGCACCT ACTGTGGGTT 
TATTCTGGCA GAAGAGGAGT GCATTGTTGG GTGTGTGATG AAGCAGCCAG GAAGCTCTCT 
GTAGCAGCTC GCTCTGCTGT GGCAGAATAT CTCAGCCTGA TCAAGGGTGG TGAAGAGACG 
GTGAAAAAAG TAGTGCTGAC GGATCCAATT CACCCTTTCA TCAGAGAGTC TTTAGCAGTG 
GTGGAGCGCT ACTTTCCCAG ATATGCACTA CAGGAGCAGG ACATACTCGG CCGCAAGGAG 
TCTGTAGACA AAGTGTTGTC GCTCGTCCCT GAAGATGTAA GGAAAGAGCT ACAGCAGGAT 
TTCCAAAATG AGAAGAAGCC AGAGAGTCGT TGGAAGCTGG TCAGGGACCA AGCCACTAAG 
AAGCAGGCTA CTGCCAAAAA GGGCCAGTAC TTTGAAAAAG AAATCATGCT GCAGTATTGT 
TACCCGCGGC TCGACGTGAA CGTCAGTAAA GGCGTGAACC ATCTGCTGAA GAGTCCTTTC 
AGCGTCCATC CCAAAACAGG ACGCATCTCT GTTCCCATTA ACCTCAAAGA ATTAGATGTG 
TTTGATCCTT TTGCTGTACC CACAATCAGC CTGATCTGTG AGGAGTTGGA TCGTCCCAGG 
GCGGGCGAAG ATGAAGAGAA ATCGGAGGAA TCTAAGGACA AGGAAAATGA GAAGGATGCA 
GCAGAGAGGC GGAAGATTAG AGATTACAAG CGGACTACCT TGGCAAAGTA TGTGAAATAC 
TTCGATCAGT TTCTCGATGC GATGGCTCGT TCATGGAAAG GAGAACTTCT CAGAAAGAGT 
GATCTTCAGA AAGAATTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023128919.1
CDS	310..1590
Translation	MPSSDYDPASLPDLLPLYYRRLFPFSQYYRWLNYGGVQKNYFQNREFSFTLKDDIYVRYQSFTTQNELEKEMQKMNPYKIDIGAIYSHRPNQHNTVKSGTFQALEKELVFDIDMTDYDDVRSCCSAADICCKCWTLMTIAIRILDRALREDFGFQHLLWVYSGRRGVHCWVCDEAARKLSVAARSAVAEYLSLIKGGEETVKKVVLTDPIHPFIRESLAVVERYFPRYALQEQDILGRKESVDKVLSLVPEDVRKELQQDFQNEKKPESRWKLVRDQATKKQATAKKGQYFEKEIMLQYCYPRLDVNVSKGVNHLLKSPFSVHPKTGRISVPINLKELDVFDPFAVPTISLICEELDRPRAGEDEEKSEESKDKENEKDAAERRKIRDYKRTTLAKYVKYFDQFLDAMARSWKGELLRKSDLQKEF

Target ORF information:

RefSeq Version	XM_023273151.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris DNA primase subunit 1 (prim1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023273151.1

ORF Insert Sequence:

ATGCCGTCCT CAGACTACGA CCCGGCGTCT CTACCCGACT TATTACCGCT GTACTACCGC 
CGGCTGTTCC CCTTCTCACA GTATTACCGC TGGCTCAACT ATGGAGGAGT GCAGAAGAAC 
TACTTTCAGA ATCGAGAGTT TTCCTTCACG CTCAAAGACG ACATCTACGT CCGCTACCAG 
TCGTTCACCA CCCAGAATGA GCTGGAGAAG GAAATGCAGA AGATGAACCC CTACAAGATT 
GACATCGGAG CAATATACAG TCACAGGCCG AATCAACACA ACACTGTGAA GTCAGGGACC 
TTCCAGGCTC TGGAGAAGGA GCTGGTGTTT GATATTGATA TGACAGATTA TGATGATGTC 
CGAAGCTGCT GCAGCGCTGC AGACATTTGC TGCAAGTGCT GGACTTTGAT GACCATCGCT 
ATTCGTATCC TGGATCGAGC TCTTCGAGAG GACTTTGGTT TCCAGCACCT ACTGTGGGTT 
TATTCTGGCA GAAGAGGAGT GCATTGTTGG GTGTGTGATG AAGCAGCCAG GAAGCTCTCT 
GTAGCAGCTC GCTCTGCTGT GGCAGAATAT CTCAGCCTGA TCAAGGGTGG TGAAGAGACG 
GTGAAAAAAG TAGTGCTGAC GGATCCAATT CACCCTTTCA TCAGAGAGTC TTTAGCAGTG 
GTGGAGCGCT ACTTTCCCAG ATATGCACTA CAGGAGCAGG ACATACTCGG CCGCAAGGAG 
TCTGTAGACA AAGTGTTGTC GCTCGTCCCT GAAGATGTAA GGAAAGAGCT ACAGCAGGAT 
TTCCAAAATG AGAAGAAGCC AGAGAGTCGT TGGAAGCTGG TCAGGGACCA AGCCACTAAG 
AAGCAGGCTA CTGCCAAAAA GGGCCAGTAC TTTGAAAAAG AAATCATGCT GCAGTATTGT 
TACCCGCGGC TCGACGTGAA CGTCAGTAAA GGCGTGAACC ATCTGCTGAA GAGTCCTTTC 
AGCGTCCATC CCAAAACAGG ACGCATCTCT GTTCCCATTA ACCTCAAAGA ATTAGATGTG 
TTTGATCCTT TTGCTGTACC CACAATCAGC CTGATCTGTG AGGAGTTGGA TCGTCCCAGG 
GCGGGCGAAG ATGAAGAGAA ATCGGAGGAA TCTAAGGACA AGGAAAATGA GAAGGATGCA 
GCAGAGAGGC GGAAGATTAG AGATTACAAG CGGACTACCT TGGCAAAGTA TGTGAAATAC 
TTCGATCAGT TTCTCGATGC GATGGCTCGT TCATGGAAAG GAGAACTTCT CAGAAAGAGT 
GATCTTCAGA AAGAATTCTG A