chfr cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	chfr
Entrez Gene ID	111569441
Full Name	checkpoint with forkhead and ring finger domains
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023271563.1	XP_023127331.1	E3 ubiquitin-protein ligase CHFR

Pathways from BioSystems
Gene Ontology

The following chfr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chfr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm78879	XM_023271563.1 Latest version!	Amphiprion ocellaris checkpoint with forkhead and ring finger domains (chfr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chfr ( XM_023271563.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023127331.1 Amphiprion ocellaris checkpoint with forkhead and ring finger domains (chfr), mRNA.

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CloneID	OAm78879
Clone ID Related Accession (Same CDS sequence)	XM_023271563.1
Accession Version	XM_023271563.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	E3 ubiquitin-protein ligase CHFR
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019372203.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGTG AGAGAAGAGG ACGACCATGG GGGAAGCTAG TCAAGGTGGA CTCCAGTGAA 
ACTATCATGC TTTTCAGCAG GGAATGCACA GTTGGCAGAA AAAAAGGATG CTATCTGTCC 
TTTCCAGCCA GTAAACTTGT CTCAGGGGAG CACTGCAAGA TTGTGAAAGA TGAAAGCTCG 
GGGCTGGTGT GGCTTGAAGA CATGAGCACT AATGGCACGG TGATCAACAT GTCCAAACTG 
GTCAAGAAGC AAACTCACAT GCTGCAAAGT GGCGATGTCA TCTACTTTGT GTACAGGAAG 
AGTGAGCCAG AGCAAAACAT TGCCTATGTT TACCACTCAA TCAAAACGGA GCGAGCTGCT 
TCACCACACA GCTATGACAC TGAGAGATCA GCCCACAGTC CTTCTCCCGT TCCAGCCTCA 
GAGATGTCTC TTTCAGTTGA GCCTGTAATG CTTGCAAAGC CTCCTCAAGA TCCAGCGCAG 
GAGGAACCTC AGCCATCCAC CTCCGCTTCA CACTTCTGTA TTGAGAGCCC TTCCTCTTCT 
GGTCCCATGG CAACCACTGC CTCTCCTGCC TCTGGTCACA CTAAAGATGC TTCCACTGCA 
GAGGAAAAAG ACACAGACAA CCTGGAGCCA GAAAGCAAGA GGAGGAAAAC TGATGATGGA 
AAAGATTATG ATTTGGGCTT GCCACACACC TCCAGAATAG AAGTGGTTGG CTCAACTAAG 
GGAAATCTTG GGAATCTCTT GTCTAAACCA CCAGTGGAAG GGACGAAAAC TGACAAAATG 
GAGGAAAGCC TGACATGTGT TATTTGTCAG GACCTGCTGC ATGACTGTGT CAGCTTGCAG 
CCTTGCATGC ACGTCTTCTG TGCTGCCTGC TACTCAGGCT GGATGGAGCG TTCTTCTCTT 
TGCCCCACCT GCCGCTGCCC TGTGGAAAGG ATCCGTAAGA ACCACATCCT CAACAACCTG 
GTGGAGGCCT ATCTCATCCA GCACCCAGAA AAGTGTCGCA GTGAGGAGGA CCTGAAAAGC 
ATGGACAGCC GTAACAAGAT AACTCAGGAC ATGTTGCAGC CAAAAGTTGA GCGGTCTTTC 
TCTGATGAGG AGGGCAGTTC AGATTACCTC TTCGAGCTCT CCGACAATGA TAGTGACTCC 
TCAGACATTA GTCAGCCTCT GGTGATGTGC AGGCAGTGTC CTGGCTACAT GAGGGACGTC 
AGCCAAATGC TGTTCTCCAC AAGTTCAAAC TACTGGTTCC CTGGTCTGCC ATCTCTACAA 
CCTGTACCTC CACCGCCCAA ACCAGCAACT GACGAGGGAG CAGTGAAGCC TGCAGGGGAG 
CAGCCCTCAA CATCCTCTGA TATTCCTTCA GCTCCTCAGG AGTACCGCTG CTCCCCTCAG 
GGATGCCATC TCATTTGCAC CTGCTGCCTC CAGCCAATGC CAGACAGACG CGCTGAACTA 
AACAACCAGC AGGTTATTGC TCAACAATGT GTGCTGTGCC AGCGACCTTT CTGTCATATG 
TACTGGGGCT GCCAGAGGAT TGGCTGTCAA GGCTGTCTGG TTCGATTTAG TGAGCTCAAT 
CTGACTGACA AATGTCTGGA TGGTGTGCTG AACAACAACA ACTACGAATC AGAAGTCCTC 
CAAAACTACC TGTCCTCCAA GGGAAAGTCA TGGAAAGATC TGCTCAAAGA GTCTCTGCAA 
GGCTTACAGC AGGGAAGCTA CTTCTTGACA GATCGTCGTG TCTCTGCAAA CACTATCCTG 
TGCTTCTGCT GTGGCCTGGG GGCCTTCAAA GAGCTGGCCT ACAAGTACAG ACAGAACATC 
CCTCCGTCTG AACTACCGGC CGGTGTAACG TCTCGCCCCG ACTGTTACTG GGGGCGCAAC 
TGTCGTACGC AGGTGAAGGC ACATCATGCA ATGAAATTCA ACCACATATG TGAGCAGACA 
CGTTTCAAGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023127331.1
CDS	127..2061
Translation	MDSERRGRPWGKLVKVDSSETIMLFSRECTVGRKKGCYLSFPASKLVSGEHCKIVKDESSGLVWLEDMSTNGTVINMSKLVKKQTHMLQSGDVIYFVYRKSEPEQNIAYVYHSIKTERAASPHSYDTERSAHSPSPVPASEMSLSVEPVMLAKPPQDPAQEEPQPSTSASHFCIESPSSSGPMATTASPASGHTKDASTAEEKDTDNLEPESKRRKTDDGKDYDLGLPHTSRIEVVGSTKGNLGNLLSKPPVEGTKTDKMEESLTCVICQDLLHDCVSLQPCMHVFCAACYSGWMERSSLCPTCRCPVERIRKNHILNNLVEAYLIQHPEKCRSEEDLKSMDSRNKITQDMLQPKVERSFSDEEGSSDYLFELSDNDSDSSDISQPLVMCRQCPGYMRDVSQMLFSTSSNYWFPGLPSLQPVPPPPKPATDEGAVKPAGEQPSTSSDIPSAPQEYRCSPQGCHLICTCCLQPMPDRRAELNNQQVIAQQCVLCQRPFCHMYWGCQRIGCQGCLVRFSELNLTDKCLDGVLNNNNYESEVLQNYLSSKGKSWKDLLKESLQGLQQGSYFLTDRRVSANTILCFCCGLGAFKELAYKYRQNIPPSELPAGVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKS

Target ORF information:

RefSeq Version	XM_023271563.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris checkpoint with forkhead and ring finger domains (chfr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023271563.1

ORF Insert Sequence:

ATGGACAGTG AGAGAAGAGG ACGACCATGG GGGAAGCTAG TCAAGGTGGA CTCCAGTGAA 
ACTATCATGC TTTTCAGCAG GGAATGCACA GTTGGCAGAA AAAAAGGATG CTATCTGTCC 
TTTCCAGCCA GTAAACTTGT CTCAGGGGAG CACTGCAAGA TTGTGAAAGA TGAAAGCTCG 
GGGCTGGTGT GGCTTGAAGA CATGAGCACT AATGGCACGG TGATCAACAT GTCCAAACTG 
GTCAAGAAGC AAACTCACAT GCTGCAAAGT GGCGATGTCA TCTACTTTGT GTACAGGAAG 
AGTGAGCCAG AGCAAAACAT TGCCTATGTT TACCACTCAA TCAAAACGGA GCGAGCTGCT 
TCACCACACA GCTATGACAC TGAGAGATCA GCCCACAGTC CTTCTCCCGT TCCAGCCTCA 
GAGATGTCTC TTTCAGTTGA GCCTGTAATG CTTGCAAAGC CTCCTCAAGA TCCAGCGCAG 
GAGGAACCTC AGCCATCCAC CTCCGCTTCA CACTTCTGTA TTGAGAGCCC TTCCTCTTCT 
GGTCCCATGG CAACCACTGC CTCTCCTGCC TCTGGTCACA CTAAAGATGC TTCCACTGCA 
GAGGAAAAAG ACACAGACAA CCTGGAGCCA GAAAGCAAGA GGAGGAAAAC TGATGATGGA 
AAAGATTATG ATTTGGGCTT GCCACACACC TCCAGAATAG AAGTGGTTGG CTCAACTAAG 
GGAAATCTTG GGAATCTCTT GTCTAAACCA CCAGTGGAAG GGACGAAAAC TGACAAAATG 
GAGGAAAGCC TGACATGTGT TATTTGTCAG GACCTGCTGC ATGACTGTGT CAGCTTGCAG 
CCTTGCATGC ACGTCTTCTG TGCTGCCTGC TACTCAGGCT GGATGGAGCG TTCTTCTCTT 
TGCCCCACCT GCCGCTGCCC TGTGGAAAGG ATCCGTAAGA ACCACATCCT CAACAACCTG 
GTGGAGGCCT ATCTCATCCA GCACCCAGAA AAGTGTCGCA GTGAGGAGGA CCTGAAAAGC 
ATGGACAGCC GTAACAAGAT AACTCAGGAC ATGTTGCAGC CAAAAGTTGA GCGGTCTTTC 
TCTGATGAGG AGGGCAGTTC AGATTACCTC TTCGAGCTCT CCGACAATGA TAGTGACTCC 
TCAGACATTA GTCAGCCTCT GGTGATGTGC AGGCAGTGTC CTGGCTACAT GAGGGACGTC 
AGCCAAATGC TGTTCTCCAC AAGTTCAAAC TACTGGTTCC CTGGTCTGCC ATCTCTACAA 
CCTGTACCTC CACCGCCCAA ACCAGCAACT GACGAGGGAG CAGTGAAGCC TGCAGGGGAG 
CAGCCCTCAA CATCCTCTGA TATTCCTTCA GCTCCTCAGG AGTACCGCTG CTCCCCTCAG 
GGATGCCATC TCATTTGCAC CTGCTGCCTC CAGCCAATGC CAGACAGACG CGCTGAACTA 
AACAACCAGC AGGTTATTGC TCAACAATGT GTGCTGTGCC AGCGACCTTT CTGTCATATG 
TACTGGGGCT GCCAGAGGAT TGGCTGTCAA GGCTGTCTGG TTCGATTTAG TGAGCTCAAT 
CTGACTGACA AATGTCTGGA TGGTGTGCTG AACAACAACA ACTACGAATC AGAAGTCCTC 
CAAAACTACC TGTCCTCCAA GGGAAAGTCA TGGAAAGATC TGCTCAAAGA GTCTCTGCAA 
GGCTTACAGC AGGGAAGCTA CTTCTTGACA GATCGTCGTG TCTCTGCAAA CACTATCCTG 
TGCTTCTGCT GTGGCCTGGG GGCCTTCAAA GAGCTGGCCT ACAAGTACAG ACAGAACATC 
CCTCCGTCTG AACTACCGGC CGGTGTAACG TCTCGCCCCG ACTGTTACTG GGGGCGCAAC 
TGTCGTACGC AGGTGAAGGC ACATCATGCA ATGAAATTCA ACCACATATG TGAGCAGACA 
CGTTTCAAGA GCTGA