PCK2 cDNA ORF clone, Alligator mississippiensis(American alligator)

Gene
Clones
gRNAs

Summary

Gene Symbol	PCK2
Entrez Gene ID	106737103
Full Name	phosphoenolpyruvate carboxykinase 2, mitochondrial
Gene Type	protein-coding
Organism	Alligator mississippiensis(American alligator)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014604508.2	XP_014459994.2	phosphoenolpyruvate carboxykinase [GTP], mitochondrial

Pathways from BioSystems
Gene Ontology

The following PCK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAl77230	XM_014604508.2 Latest version!	Alligator mississippiensis phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PCK2 ( XM_014604508.2 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_014459994.2 Alligator mississippiensis phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), mRNA.

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CloneID	OAl77230
Clone ID Related Accession (Same CDS sequence)	XM_014604508.2
Accession Version	XM_014604508.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	phosphoenolpyruvate carboxykinase [GTP], mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017714006.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 9, 2016 this sequence version replaced XM_014604508.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCGC CGGGCACTCG CCTGCTGGGG CAGGGCATGC GCTGGCTGCG GGGCTGCCGG 
GCGGCATGGG CGCGGGGCAC GCACACAGCC CTGCGCGTGG TGCACGGGGA CCTGGAGGCA 
GCGCCGGCGC GGGTGCGAGC CTTCGTGGAG CAGGGTGCCC AGCTGTGCCA ACCGGCTGCC 
GTGCACCTGT GCGATGGGTC GCAGGCCGAG AATGGTGCTG TGCTGGCCCT GCTGGAGACT 
GAGGGTGTCC TCCAGCGCCT CGCCAAGTAT GAGAACTGCT GGCTGGCACG GACTGACCCC 
AAGGACGTGG CGCGGGTGGA GAGCAAGACG GTGATTGTGA CAGAGCAGCA GCGGGACACG 
GTGCCTATCC CAGCTTCTGG TGCCACCGGG CAGCTCGGCA ATTGGATGAG CCCTGCTGAC 
TTCCAGGCTG CGGTGGACAA CCGCTTCCCT GGCTGCATGA GAGGGCGCAC CATGTATGTG 
ATCCCATACA GCATGGGTCC GCTGGGCTCG CCGCTGGCCA AGACGGGCGT GCAGCTGACG 
GACTCACCCT ACGTGGTGGC CAGCATGCGC ATCATGACCC GAATGGGTGC CCCTGCCCTG 
CGCGCCCTTG GTGATGGGGA GTTTGTGCAG TGCCTGCACT CCGTGGGACA GCCACTGCCC 
CTGCAAGAGG AGCTGGTGAA CAACTGGCCC TGTAACCCGG CCAAGACACT GATTGCACAT 
GTGCCTGACC GGCGGGAGAT CGTTTCCTTC GGCAGTGGCT ACGGTGGCAA CTCCCTGCTG 
GGCAAGAAGT GCTTTGCCCT GCGCATCGCC TCGCGCATTG CCCGCGATGA GGGCTGGCTG 
GCTGAGCACA TGCTGATCCT GGGCCTCACC AGCCCTGAGG GGCGGAAGAA GTACGTGGCG 
GCCGCATTCC CCAGCGCCTG CGGCAAGACC AACCTGGCCA TGATGACGCC ATCGCTGCCC 
GGCTGGCGCG CTGAGTGTGT GGGCGACGAC ATTGCCTGGA TGAAGTTTGA CAGTGAGGGA 
CGTCTGCGGG CCATCAATCC TGAAAGTGGG TTCTTCGGGG TGGCGCCAGG CACATCAATG 
CGCACCAACC CCAACGCCAT GCACACAGTG CAGCGCAACA CGCTCTTCAC CAACGTGGGG 
CTCACCGGCG AGGGCGGCGT CTACTGGGAG GGCATCGACC ACCACCTGCC CCCTCGCACA 
CCCATCTATG ACTGGCAGGG CCGGCCCTGG AGCCCTGATG CCAAGGAGCC ATGTGCCCAC 
CCCAACTCGC GGTTCTGCGC CCCTGCCCGC CAGTGCCCCA TCATGGACCC AGCTTGGGAA 
TCTCCTGAGG GAGTCCCCAT TGATGCCATC ATCTTCGGTG GCCGGAGACC CCAAGGGGTG 
CCGCTAGTCT ATGAGGCCTT CAACTGGCAG CATGGTGTCT TTGTGGGCAG TGCCATGCGT 
TCCGAGTCCA CCGCTGCCGC CGAGCACAAA GGCAAGGTGA TCATGCACGA CCCCTTCGCC 
ATGCGTCCTT TCTTCGGGTA CAACTTTGGG CGGTACCTGG AACACTGGCT GGCCGTGGGG 
GAGCGGAGCG GCGCCCGCCT GCCCAAGATC TTCCACGTCA ACTGGTTCCG CAAGGGCCCC 
GATGGCAATT TCCTGTGGCC CGGCTTCGGC GAGAACTGCC GTGTGCTGGA CTGGATCTGT 
CGGCGTGTGG ATGGAGAGGA CAGTGCCCAG GAGACACCCA TTGGCTATGT TCCCCGCGAG 
GGGGCCCTCA ATCTCCAGGG CTTGGCCAAC GTGGACTACC AGGAGCTCTT CCACCTGCCC 
AAGGAATTCT GGGAGCAGGA GGCGCGGGAC ATACGCAAGT ACCTCACGGA GCAGGTCAAC 
GAGGACCTGC CCCGCGAGGT GATGCAGGAG CTGGAGGGGC TGGAGAGCCG TGTGAAGAAG 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014459994.2
CDS	74..1999
Translation	MAPPGTRLLGQGMRWLRGCRAAWARGTHTALRVVHGDLEAAPARVRAFVEQGAQLCQPAAVHLCDGSQAENGAVLALLETEGVLQRLAKYENCWLARTDPKDVARVESKTVIVTEQQRDTVPIPASGATGQLGNWMSPADFQAAVDNRFPGCMRGRTMYVIPYSMGPLGSPLAKTGVQLTDSPYVVASMRIMTRMGAPALRALGDGEFVQCLHSVGQPLPLQEELVNNWPCNPAKTLIAHVPDRREIVSFGSGYGGNSLLGKKCFALRIASRIARDEGWLAEHMLILGLTSPEGRKKYVAAAFPSACGKTNLAMMTPSLPGWRAECVGDDIAWMKFDSEGRLRAINPESGFFGVAPGTSMRTNPNAMHTVQRNTLFTNVGLTGEGGVYWEGIDHHLPPRTPIYDWQGRPWSPDAKEPCAHPNSRFCAPARQCPIMDPAWESPEGVPIDAIIFGGRRPQGVPLVYEAFNWQHGVFVGSAMRSESTAAAEHKGKVIMHDPFAMRPFFGYNFGRYLEHWLAVGERSGARLPKIFHVNWFRKGPDGNFLWPGFGENCRVLDWICRRVDGEDSAQETPIGYVPREGALNLQGLANVDYQELFHLPKEFWEQEARDIRKYLTEQVNEDLPREVMQELEGLESRVKKM

Target ORF information:

RefSeq Version	XM_014604508.2
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014604508.2

ORF Insert Sequence:

ATGGCGCCGC CGGGCACTCG CCTGCTGGGG CAGGGCATGC GCTGGCTGCG GGGCTGCCGG 
GCGGCATGGG CGCGGGGCAC GCACACAGCC CTGCGCGTGG TGCACGGGGA CCTGGAGGCA 
GCGCCGGCGC GGGTGCGAGC CTTCGTGGAG CAGGGTGCCC AGCTGTGCCA ACCGGCTGCC 
GTGCACCTGT GCGATGGGTC GCAGGCCGAG AATGGTGCTG TGCTGGCCCT GCTGGAGACT 
GAGGGTGTCC TCCAGCGCCT CGCCAAGTAT GAGAACTGCT GGCTGGCACG GACTGACCCC 
AAGGACGTGG CGCGGGTGGA GAGCAAGACG GTGATTGTGA CAGAGCAGCA GCGGGACACG 
GTGCCTATCC CAGCTTCTGG TGCCACCGGG CAGCTCGGCA ATTGGATGAG CCCTGCTGAC 
TTCCAGGCTG CGGTGGACAA CCGCTTCCCT GGCTGCATGA GAGGGCGCAC CATGTATGTG 
ATCCCATACA GCATGGGTCC GCTGGGCTCG CCGCTGGCCA AGACGGGCGT GCAGCTGACG 
GACTCACCCT ACGTGGTGGC CAGCATGCGC ATCATGACCC GAATGGGTGC CCCTGCCCTG 
CGCGCCCTTG GTGATGGGGA GTTTGTGCAG TGCCTGCACT CCGTGGGACA GCCACTGCCC 
CTGCAAGAGG AGCTGGTGAA CAACTGGCCC TGTAACCCGG CCAAGACACT GATTGCACAT 
GTGCCTGACC GGCGGGAGAT CGTTTCCTTC GGCAGTGGCT ACGGTGGCAA CTCCCTGCTG 
GGCAAGAAGT GCTTTGCCCT GCGCATCGCC TCGCGCATTG CCCGCGATGA GGGCTGGCTG 
GCTGAGCACA TGCTGATCCT GGGCCTCACC AGCCCTGAGG GGCGGAAGAA GTACGTGGCG 
GCCGCATTCC CCAGCGCCTG CGGCAAGACC AACCTGGCCA TGATGACGCC ATCGCTGCCC 
GGCTGGCGCG CTGAGTGTGT GGGCGACGAC ATTGCCTGGA TGAAGTTTGA CAGTGAGGGA 
CGTCTGCGGG CCATCAATCC TGAAAGTGGG TTCTTCGGGG TGGCGCCAGG CACATCAATG 
CGCACCAACC CCAACGCCAT GCACACAGTG CAGCGCAACA CGCTCTTCAC CAACGTGGGG 
CTCACCGGCG AGGGCGGCGT CTACTGGGAG GGCATCGACC ACCACCTGCC CCCTCGCACA 
CCCATCTATG ACTGGCAGGG CCGGCCCTGG AGCCCTGATG CCAAGGAGCC ATGTGCCCAC 
CCCAACTCGC GGTTCTGCGC CCCTGCCCGC CAGTGCCCCA TCATGGACCC AGCTTGGGAA 
TCTCCTGAGG GAGTCCCCAT TGATGCCATC ATCTTCGGTG GCCGGAGACC CCAAGGGGTG 
CCGCTAGTCT ATGAGGCCTT CAACTGGCAG CATGGTGTCT TTGTGGGCAG TGCCATGCGT 
TCCGAGTCCA CCGCTGCCGC CGAGCACAAA GGCAAGGTGA TCATGCACGA CCCCTTCGCC 
ATGCGTCCTT TCTTCGGGTA CAACTTTGGG CGGTACCTGG AACACTGGCT GGCCGTGGGG 
GAGCGGAGCG GCGCCCGCCT GCCCAAGATC TTCCACGTCA ACTGGTTCCG CAAGGGCCCC 
GATGGCAATT TCCTGTGGCC CGGCTTCGGC GAGAACTGCC GTGTGCTGGA CTGGATCTGT 
CGGCGTGTGG ATGGAGAGGA CAGTGCCCAG GAGACACCCA TTGGCTATGT TCCCCGCGAG 
GGGGCCCTCA ATCTCCAGGG CTTGGCCAAC GTGGACTACC AGGAGCTCTT CCACCTGCCC 
AAGGAATTCT GGGAGCAGGA GGCGCGGGAC ATACGCAAGT ACCTCACGGA GCAGGTCAAC 
GAGGACCTGC CCCGCGAGGT GATGCAGGAG CTGGAGGGGC TGGAGAGCCG TGTGAAGAAG 
ATGTGA