BRF1 cDNA ORF clone, Alligator mississippiensis(American alligator)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRF1
Entrez Gene ID	102565700
Full Name	BRF1, RNA polymerase III transcription initiation factor subunit
Gene Type	protein-coding
Organism	Alligator mississippiensis(American alligator)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014598772.2	XP_014454258.2	transcription factor IIIB 90 kDa subunit isoform X1
XM_019479955.1	XP_019335500.1	transcription factor IIIB 90 kDa subunit isoform X3
XM_019479954.1	XP_019335499.1	transcription factor IIIB 90 kDa subunit isoform X2

Pathways from BioSystems
Gene Ontology

The following BRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAl78226	XM_014598772.2 Latest version!	Alligator mississippiensis BRF1, RNA polymerase III transcription initiation factor subunit (BRF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OAl78227	XM_019479954.1 Latest version!	Alligator mississippiensis BRF1, RNA polymerase III transcription initiation factor subunit (BRF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OAl78228	XM_019479955.1 Latest version!	Alligator mississippiensis BRF1, RNA polymerase III transcription initiation factor subunit (BRF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRF1 ( XM_014598772.2 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_014454258.2 Alligator mississippiensis BRF1, RNA polymerase III transcription initiation factor subunit (BRF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAl78226
Clone ID Related Accession (Same CDS sequence)	XM_014598772.2
Accession Version	XM_014598772.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	transcription factor IIIB 90 kDa subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017714221.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 9, 2016 this sequence version replaced XM_014598772.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGCG GGCGGGTGTG CGCGGCGTGC GGCAGCGCCG ACATCGAGGT GGACGCGGCG 
CGGGGCGACG CCGTGTGCAC GGGCTGCGGC TCCGTGCTCG AGGACAACAT CATCGTCTCC 
GAGGTGCAGT TCGTGGAGAC CGGCGGCGGC GGCGCCTCGG CCGTGGGGCA GTTCGTGTCC 
CTTGACGGTC CGGGTAAAAC ACCGTTGATA GGAGGAGGAT TTCATGCCAA TTTGGGAAAG 
GAATCGCGAG CACAGACTCT ACAGAACGGG AAACGCCAGA TTCACCATTT GGGAAACCAG 
CTGCAACTCA ACCAGCACTG TCTTGATACT GCCTTTAATT TTTTCAAGAT GGCAGTGAGC 
AAGCACCTCA CCCGTGGAAG AAAGATGACT CACGTAATTG CTGCGTGTCT CTACTTGGTG 
TGTAGAACAG AAGGAACTCC TCACATGTTA CTTGACCTCA GTGACCTCCT TCAGGTGAAT 
GTCTATGTGC TGGGAAAAAC TTTTCTTCTG TTGGCCAGAG AGCTCTGCAT AAATGCTCCA 
GCTATAGATC CTTGTTTATA TATTCCTCGT TTTGCACACA TGCTAGAATT TGGAGATAAG 
AACCATGAGG TATCGATGAC GGCTCTAAGG TTATTACAGA GGATGAAGAG AGACTGGATG 
CACACAGGAC GTCGACCGTC AGGGTTGTGT GGAGCAGCCC TTCTAGTGGC AGCAAGAATG 
CATGATTTCC GACGTACTGT TAAGGAGGTC ATCAGAGTGG TCAAGGTTTG TGAGTCTACG 
TTACGGAAAA GGCTTACAGA ATTTGAAGAT ACCCCAACAA GTCAGCTCAC GATAGATGAA 
TTTATGAAGA TTGATTTGGA AGGGGAATGT GATCCTCCTT CGTTTACAGC TGGTCAAAGA 
AAACTAAAGC TGCAGCAGCT CGAGAAGGAG CTGTCAAAAA AGCTTGAAGA TTATGAAGGT 
GAAATATCAA TTTACCAAGA TGAAATAGAG ATTGAATTGG AAAATAGTAG GCCAAAGGCA 
AAAGGAGTAT TTGCAAATTA CACTAAAGAC GATTCTATAG AAGATAATAC TTCTAGCATA 
TTTGGAGAGG AGGAGGTAGA AGATGAAGAA CTGGAAGCAG CTGCTAATCA TTTAAACAAG 
GACTTTTGTA ATGAAATTCT TGACAAAGAC AGATTAAAGA CAAATGAAGA TAGAGAATGC 
AGAAATGGGA ACGCACCTAC TGTAAGACCA CCTGCGCTGG AATCCTTGCT TGGCCCACTC 
CCTACTGCAG CTAGCCTTGG AATAACAGAG TCCATAAAAG TTTGTATCTC CACAAAGGAG 
CGAGTGCCCA ATGAAAATAC AGGAGATGGT GAATTAGATT TGAGTGGAAT TGATGACAGT 
GAAATAGACA GGTATATACT TAATGAAACA GAAGCTCAGA TAAAAGCTGA ACTGTGGATG 
AGAGAAAACG CAGATTATTT GAAGGAACAA AAAGAAAAAG AAGCAAGGAT AGCAAAAGAG 
AAGGAGCTTG GGATTTATAA GGAACAAAAG CCAAAGAAAT CTGCAAAAAA ACGGGAGCCA 
ATTCAAGCCA GCACAGCAGG AGAAGCAATT GAAAAGATGT TAGAACAGAA GAAAATTTCA 
AGTAAAATTA ATTATAATGT GCTAAGGGAC CTGAACAGCA AAGGAAGTAG CACACCAAAG 
AAAGAGGATG ACAGCACTGA TGACAGCAGC AACACCAAGA AGCTGTCAAG AAGAAAATCT 
ATTGCCAGTA GGAATTTAGC CAACTCTGTA AACACTGTGG GAAAAAGATT AAGACCCTTG 
ATTTCTTCAC AACTAGCTAA AAAGGCTGCA ACTGAAGAGG TTACCTTTTC AGACTTAAAG 
CCAGAAGTGC AGGAGTCGGG AAAAGCAGAG AGTGTGCTGG TGGAGAGTGG ACCAGTGTCC 
TATAACCATG ACGATGATGC TGAAGAAGAA GAAGTAGAAG AGGAGGATGA TCATTGCATG 
AGTGCCCTTC AGTTAATGGG AGGAAATGAT TATGGCTGTG ATATGGATGA CGATGATGGC 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014454258.2
CDS	13..2058
Translation	MPGGRVCAACGSADIEVDAARGDAVCTGCGSVLEDNIIVSEVQFVETGGGGASAVGQFVSLDGPGKTPLIGGGFHANLGKESRAQTLQNGKRQIHHLGNQLQLNQHCLDTAFNFFKMAVSKHLTRGRKMTHVIAACLYLVCRTEGTPHMLLDLSDLLQVNVYVLGKTFLLLARELCINAPAIDPCLYIPRFAHMLEFGDKNHEVSMTALRLLQRMKRDWMHTGRRPSGLCGAALLVAARMHDFRRTVKEVIRVVKVCESTLRKRLTEFEDTPTSQLTIDEFMKIDLEGECDPPSFTAGQRKLKLQQLEKELSKKLEDYEGEISIYQDEIEIELENSRPKAKGVFANYTKDDSIEDNTSSIFGEEEVEDEELEAAANHLNKDFCNEILDKDRLKTNEDRECRNGNAPTVRPPALESLLGPLPTAASLGITESIKVCISTKERVPNENTGDGELDLSGIDDSEIDRYILNETEAQIKAELWMRENADYLKEQKEKEARIAKEKELGIYKEQKPKKSAKKREPIQASTAGEAIEKMLEQKKISSKINYNVLRDLNSKGSSTPKKEDDSTDDSSNTKKLSRRKSIASRNLANSVNTVGKRLRPLISSQLAKKAATEEVTFSDLKPEVQESGKAESVLVESGPVSYNHDDDAEEEEVEEEDDHCMSALQLMGGNDYGCDMDDDDGY

Target ORF information:

RefSeq Version	XM_014598772.2
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis BRF1, RNA polymerase III transcription initiation factor subunit (BRF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014598772.2

ORF Insert Sequence:

ATGCCGGGCG GGCGGGTGTG CGCGGCGTGC GGCAGCGCCG ACATCGAGGT GGACGCGGCG 
CGGGGCGACG CCGTGTGCAC GGGCTGCGGC TCCGTGCTCG AGGACAACAT CATCGTCTCC 
GAGGTGCAGT TCGTGGAGAC CGGCGGCGGC GGCGCCTCGG CCGTGGGGCA GTTCGTGTCC 
CTTGACGGTC CGGGTAAAAC ACCGTTGATA GGAGGAGGAT TTCATGCCAA TTTGGGAAAG 
GAATCGCGAG CACAGACTCT ACAGAACGGG AAACGCCAGA TTCACCATTT GGGAAACCAG 
CTGCAACTCA ACCAGCACTG TCTTGATACT GCCTTTAATT TTTTCAAGAT GGCAGTGAGC 
AAGCACCTCA CCCGTGGAAG AAAGATGACT CACGTAATTG CTGCGTGTCT CTACTTGGTG 
TGTAGAACAG AAGGAACTCC TCACATGTTA CTTGACCTCA GTGACCTCCT TCAGGTGAAT 
GTCTATGTGC TGGGAAAAAC TTTTCTTCTG TTGGCCAGAG AGCTCTGCAT AAATGCTCCA 
GCTATAGATC CTTGTTTATA TATTCCTCGT TTTGCACACA TGCTAGAATT TGGAGATAAG 
AACCATGAGG TATCGATGAC GGCTCTAAGG TTATTACAGA GGATGAAGAG AGACTGGATG 
CACACAGGAC GTCGACCGTC AGGGTTGTGT GGAGCAGCCC TTCTAGTGGC AGCAAGAATG 
CATGATTTCC GACGTACTGT TAAGGAGGTC ATCAGAGTGG TCAAGGTTTG TGAGTCTACG 
TTACGGAAAA GGCTTACAGA ATTTGAAGAT ACCCCAACAA GTCAGCTCAC GATAGATGAA 
TTTATGAAGA TTGATTTGGA AGGGGAATGT GATCCTCCTT CGTTTACAGC TGGTCAAAGA 
AAACTAAAGC TGCAGCAGCT CGAGAAGGAG CTGTCAAAAA AGCTTGAAGA TTATGAAGGT 
GAAATATCAA TTTACCAAGA TGAAATAGAG ATTGAATTGG AAAATAGTAG GCCAAAGGCA 
AAAGGAGTAT TTGCAAATTA CACTAAAGAC GATTCTATAG AAGATAATAC TTCTAGCATA 
TTTGGAGAGG AGGAGGTAGA AGATGAAGAA CTGGAAGCAG CTGCTAATCA TTTAAACAAG 
GACTTTTGTA ATGAAATTCT TGACAAAGAC AGATTAAAGA CAAATGAAGA TAGAGAATGC 
AGAAATGGGA ACGCACCTAC TGTAAGACCA CCTGCGCTGG AATCCTTGCT TGGCCCACTC 
CCTACTGCAG CTAGCCTTGG AATAACAGAG TCCATAAAAG TTTGTATCTC CACAAAGGAG 
CGAGTGCCCA ATGAAAATAC AGGAGATGGT GAATTAGATT TGAGTGGAAT TGATGACAGT 
GAAATAGACA GGTATATACT TAATGAAACA GAAGCTCAGA TAAAAGCTGA ACTGTGGATG 
AGAGAAAACG CAGATTATTT GAAGGAACAA AAAGAAAAAG AAGCAAGGAT AGCAAAAGAG 
AAGGAGCTTG GGATTTATAA GGAACAAAAG CCAAAGAAAT CTGCAAAAAA ACGGGAGCCA 
ATTCAAGCCA GCACAGCAGG AGAAGCAATT GAAAAGATGT TAGAACAGAA GAAAATTTCA 
AGTAAAATTA ATTATAATGT GCTAAGGGAC CTGAACAGCA AAGGAAGTAG CACACCAAAG 
AAAGAGGATG ACAGCACTGA TGACAGCAGC AACACCAAGA AGCTGTCAAG AAGAAAATCT 
ATTGCCAGTA GGAATTTAGC CAACTCTGTA AACACTGTGG GAAAAAGATT AAGACCCTTG 
ATTTCTTCAC AACTAGCTAA AAAGGCTGCA ACTGAAGAGG TTACCTTTTC AGACTTAAAG 
CCAGAAGTGC AGGAGTCGGG AAAAGCAGAG AGTGTGCTGG TGGAGAGTGG ACCAGTGTCC 
TATAACCATG ACGATGATGC TGAAGAAGAA GAAGTAGAAG AGGAGGATGA TCATTGCATG 
AGTGCCCTTC AGTTAATGGG AGGAAATGAT TATGGCTGTG ATATGGATGA CGATGATGGC 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRF1 ( XM_019479954.1 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_019335499.1 Alligator mississippiensis BRF1, RNA polymerase III transcription initiation factor subunit (BRF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAl78227
Clone ID Related Accession (Same CDS sequence)	XM_019479954.1
Accession Version	XM_019479954.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	transcription factor IIIB 90 kDa subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017714221.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGCG GGCGGGTGTG CGCGGCGTGC GGCAGCGCCG ACATCGAGGT GGACGCGGCG 
CGGGGCGACG CCGTGTGCAC GGGCTGCGGC TCCGTGCTCG AGGACAACAT CATCGTCTCC 
GAGGTGCAGT TCGTGGAGAC CGGCGGCGGC GGCGCCTCGG CCGTGGGGCA GTTCGTGTCC 
CTTGACGGTC CGGGTAAAAC ACCGTTGATA GGAGGAGGAT TTCATGCCAA TTTGGGAAAG 
GAATCGCGAG CACAGACTCT ACAGAACGGG AAACGCCAGA TTCACCATTT GGGAAACCAG 
CTGCAACTCA ACCAGCACTG TCTTGATACT GCCTTTAATT TTTTCAAGAT GGCAGTGAGC 
AAGCACCTCA CCCGTGGAAG AAAGATGACT CACGTAATTG CTGCGTGTCT CTACTTGGTG 
TGTAGAACAG AAGGAACTCC TCACATGTTA CTTGACCTCA GTGACCTCCT TCAGGTGAAT 
GTCTATGTGC TGGGAAAAAC TTTTCTTCTG TTGGCCAGAG AGCTCTGCAT AAATGCTCCA 
GCTATAGATC CTTGTTTATA TATTCCTCGT TTTGCACACA TGCTAGAATT TGGAGATAAG 
AACCATGAGG TATCGATGAC GGCTCTAAGG TTATTACAGA GGATGAAGAG AGACTGGATG 
CACACAGGAC GTCGACCGTC AGGGTTGTGT GGAGCAGCCC TTCTAGTGGC AGCAAGAATG 
CATGATTTCC GACGTACTGT TAAGGAGGTC ATCAGAGTGG TCAAGGTTTG TGAGTCTACG 
TTACGGAAAA GGCTTACAGA ATTTGAAGAT ACCCCAACAA GTCAGCTCAC GATAGATGAA 
TTTATGAAGA TTGATTTGGA AGGGGAATGT GATCCTCCTT CGTTTACAGC TGGTCAAAGA 
AAACTAAAGC TGCAGCAGCT CGAGAAGGAG CTGTCAAAAA AGCTTGAAGA TTATGAAGGT 
GAAATATCAA TTTACCAAGA TGAAATAGAG ATTGAATTGG AAAATAGTAG GCCAAAGGCA 
AAAGGAGTAT TTGCAAATTA CACTAAAGAC GATTCTATAG AAGATAATAC TTCTAGCATA 
TTTGGAGAGG AGGAGGTAGA AGATGAAGAA CTGGAAGCAG CTGCTAATCA TTTAAACAAG 
GACTTTTGTA ATGAAATTCT TGACAAAGAC AGATTAAAGA CAAATGAAGA TAGAGAATGC 
AGAAATGGGA ACGCACCTAC TGTAAGACCA CCTGCGCTGG AATCCTTGCT TGGCCCACTC 
CCTACTGCAG CTAGCCTTGG AATAACAGAG TCCATAAAAG TTTGTATCTC CACAAAGGAG 
CGAGTGCCCA ATGAAAATAC AGGAGATGGT GAATTAGATT TGAGTGGAAT TGATGACAGT 
GAAATAGACA GGTATATACT TAATGAAACA GAAGCTCAGA TAAAAGCTGA ACTGTGGATG 
AGAGAAAACG CAGATTATTT GAAGGAACAA AAAGAAAAAG AAGCAAGGAT AGCAAAAGAG 
AAGGAGCTTG GGATTTATAA GGAACAAAAG CCAAAGAAAT CTGCAAAAAA ACGGGAGCCA 
ATTCAAGCCA GCACAGCAGG AGAAGCAATT GAAAAGATGT TAGAACAGAA GAAAATTTCA 
AGTAAAATTA ATTATAATGT GCTAAGGGAC CTGAACAGCA AAGGAAGTAG CACACCAAAG 
AAAGAGGATG ACAGCACTGA TGACAGCAGC AACACCAAGA AGCTGTCAAG AAGAAAATCT 
ATTGCCAGTA GGAATTTAGC CAACTCTGTA AACACTGTGG GAAAAAGGAG TGTGGCCGTG 
CCGAGTAGAC TTCCCGTTGA AGGCAAAACT TCAGTGTATA CAAAATCACG TCATCCGAAC 
CAGCCAGAAT TTCAGCCTGC TCCAAGCACA GGAGCCATCC AAGCTGCTCA AGTGCAAGAG 
CTCTGTGGAT TTGGATCGCT GTGCAGTGAT ATTCGGAAAG TGATTCTGCA TCTGTGTGTG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019335499.1
CDS	13..1998
Translation	MPGGRVCAACGSADIEVDAARGDAVCTGCGSVLEDNIIVSEVQFVETGGGGASAVGQFVSLDGPGKTPLIGGGFHANLGKESRAQTLQNGKRQIHHLGNQLQLNQHCLDTAFNFFKMAVSKHLTRGRKMTHVIAACLYLVCRTEGTPHMLLDLSDLLQVNVYVLGKTFLLLARELCINAPAIDPCLYIPRFAHMLEFGDKNHEVSMTALRLLQRMKRDWMHTGRRPSGLCGAALLVAARMHDFRRTVKEVIRVVKVCESTLRKRLTEFEDTPTSQLTIDEFMKIDLEGECDPPSFTAGQRKLKLQQLEKELSKKLEDYEGEISIYQDEIEIELENSRPKAKGVFANYTKDDSIEDNTSSIFGEEEVEDEELEAAANHLNKDFCNEILDKDRLKTNEDRECRNGNAPTVRPPALESLLGPLPTAASLGITESIKVCISTKERVPNENTGDGELDLSGIDDSEIDRYILNETEAQIKAELWMRENADYLKEQKEKEARIAKEKELGIYKEQKPKKSAKKREPIQASTAGEAIEKMLEQKKISSKINYNVLRDLNSKGSSTPKKEDDSTDDSSNTKKLSRRKSIASRNLANSVNTVGKRSVAVPSRLPVEGKTSVYTKSRHPNQPEFQPAPSTGAIQAAQVQELCGFGSLCSDIRKVILHLCVK

Target ORF information:

RefSeq Version	XM_019479954.1
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis BRF1, RNA polymerase III transcription initiation factor subunit (BRF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019479954.1

ORF Insert Sequence:

ATGCCGGGCG GGCGGGTGTG CGCGGCGTGC GGCAGCGCCG ACATCGAGGT GGACGCGGCG 
CGGGGCGACG CCGTGTGCAC GGGCTGCGGC TCCGTGCTCG AGGACAACAT CATCGTCTCC 
GAGGTGCAGT TCGTGGAGAC CGGCGGCGGC GGCGCCTCGG CCGTGGGGCA GTTCGTGTCC 
CTTGACGGTC CGGGTAAAAC ACCGTTGATA GGAGGAGGAT TTCATGCCAA TTTGGGAAAG 
GAATCGCGAG CACAGACTCT ACAGAACGGG AAACGCCAGA TTCACCATTT GGGAAACCAG 
CTGCAACTCA ACCAGCACTG TCTTGATACT GCCTTTAATT TTTTCAAGAT GGCAGTGAGC 
AAGCACCTCA CCCGTGGAAG AAAGATGACT CACGTAATTG CTGCGTGTCT CTACTTGGTG 
TGTAGAACAG AAGGAACTCC TCACATGTTA CTTGACCTCA GTGACCTCCT TCAGGTGAAT 
GTCTATGTGC TGGGAAAAAC TTTTCTTCTG TTGGCCAGAG AGCTCTGCAT AAATGCTCCA 
GCTATAGATC CTTGTTTATA TATTCCTCGT TTTGCACACA TGCTAGAATT TGGAGATAAG 
AACCATGAGG TATCGATGAC GGCTCTAAGG TTATTACAGA GGATGAAGAG AGACTGGATG 
CACACAGGAC GTCGACCGTC AGGGTTGTGT GGAGCAGCCC TTCTAGTGGC AGCAAGAATG 
CATGATTTCC GACGTACTGT TAAGGAGGTC ATCAGAGTGG TCAAGGTTTG TGAGTCTACG 
TTACGGAAAA GGCTTACAGA ATTTGAAGAT ACCCCAACAA GTCAGCTCAC GATAGATGAA 
TTTATGAAGA TTGATTTGGA AGGGGAATGT GATCCTCCTT CGTTTACAGC TGGTCAAAGA 
AAACTAAAGC TGCAGCAGCT CGAGAAGGAG CTGTCAAAAA AGCTTGAAGA TTATGAAGGT 
GAAATATCAA TTTACCAAGA TGAAATAGAG ATTGAATTGG AAAATAGTAG GCCAAAGGCA 
AAAGGAGTAT TTGCAAATTA CACTAAAGAC GATTCTATAG AAGATAATAC TTCTAGCATA 
TTTGGAGAGG AGGAGGTAGA AGATGAAGAA CTGGAAGCAG CTGCTAATCA TTTAAACAAG 
GACTTTTGTA ATGAAATTCT TGACAAAGAC AGATTAAAGA CAAATGAAGA TAGAGAATGC 
AGAAATGGGA ACGCACCTAC TGTAAGACCA CCTGCGCTGG AATCCTTGCT TGGCCCACTC 
CCTACTGCAG CTAGCCTTGG AATAACAGAG TCCATAAAAG TTTGTATCTC CACAAAGGAG 
CGAGTGCCCA ATGAAAATAC AGGAGATGGT GAATTAGATT TGAGTGGAAT TGATGACAGT 
GAAATAGACA GGTATATACT TAATGAAACA GAAGCTCAGA TAAAAGCTGA ACTGTGGATG 
AGAGAAAACG CAGATTATTT GAAGGAACAA AAAGAAAAAG AAGCAAGGAT AGCAAAAGAG 
AAGGAGCTTG GGATTTATAA GGAACAAAAG CCAAAGAAAT CTGCAAAAAA ACGGGAGCCA 
ATTCAAGCCA GCACAGCAGG AGAAGCAATT GAAAAGATGT TAGAACAGAA GAAAATTTCA 
AGTAAAATTA ATTATAATGT GCTAAGGGAC CTGAACAGCA AAGGAAGTAG CACACCAAAG 
AAAGAGGATG ACAGCACTGA TGACAGCAGC AACACCAAGA AGCTGTCAAG AAGAAAATCT 
ATTGCCAGTA GGAATTTAGC CAACTCTGTA AACACTGTGG GAAAAAGGAG TGTGGCCGTG 
CCGAGTAGAC TTCCCGTTGA AGGCAAAACT TCAGTGTATA CAAAATCACG TCATCCGAAC 
CAGCCAGAAT TTCAGCCTGC TCCAAGCACA GGAGCCATCC AAGCTGCTCA AGTGCAAGAG 
CTCTGTGGAT TTGGATCGCT GTGCAGTGAT ATTCGGAAAG TGATTCTGCA TCTGTGTGTG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRF1 ( XM_019479955.1 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_019335500.1 Alligator mississippiensis BRF1, RNA polymerase III transcription initiation factor subunit (BRF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OAl78228
Clone ID Related Accession (Same CDS sequence)	XM_019479955.1
Accession Version	XM_019479955.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	transcription factor IIIB 90 kDa subunit isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017714221.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGTGA GCAAGCACCT CACCCGTGGA AGAAAGATGA CTCACGTAAT TGCTGCGTGT 
CTCTACTTGG TGTGTAGAAC AGAAGGAACT CCTCACATGT TACTTGACCT CAGTGACCTC 
CTTCAGGTGA ATGTCTATGT GCTGGGAAAA ACTTTTCTTC TGTTGGCCAG AGAGCTCTGC 
ATAAATGCTC CAGCTATAGA TCCTTGTTTA TATATTCCTC GTTTTGCACA CATGCTAGAA 
TTTGGAGATA AGAACCATGA GGTATCGATG ACGGCTCTAA GGTTATTACA GAGGATGAAG 
AGAGACTGGA TGCACACAGG ACGTCGACCG TCAGGGTTGT GTGGAGCAGC CCTTCTAGTG 
GCAGCAAGAA TGCATGATTT CCGACGTACT GTTAAGGAGG TCATCAGAGT GGTCAAGGTT 
TGTGAGTCTA CGTTACGGAA AAGGCTTACA GAATTTGAAG ATACCCCAAC AAGTCAGCTC 
ACGATAGATG AATTTATGAA GATTGATTTG GAAGGGGAAT GTGATCCTCC TTCGTTTACA 
GCTGGTCAAA GAAAACTAAA GCTGCAGCAG CTCGAGAAGG AGCTGTCAAA AAAGCTTGAA 
GATTATGAAG GTGAAATATC AATTTACCAA GATGAAATAG AGATTGAATT GGAAAATAGT 
AGGCCAAAGG CAAAAGGAGT ATTTGCAAAT TACACTAAAG ACGATTCTAT AGAAGATAAT 
ACTTCTAGCA TATTTGGAGA GGAGGAGGTA GAAGATGAAG AACTGGAAGC AGCTGCTAAT 
CATTTAAACA AGGACTTTTG TAATGAAATT CTTGACAAAG ACAGATTAAA GACAAATGAA 
GATAGAGAAT GCAGAAATGG GAACGCACCT ACTGTAAGAC CACCTGCGCT GGAATCCTTG 
CTTGGCCCAC TCCCTACTGC AGCTAGCCTT GGAATAACAG AGTCCATAAA AGTTTGTATC 
TCCACAAAGG AGCGAGTGCC CAATGAAAAT ACAGGAGATG GTGAATTAGA TTTGAGTGGA 
ATTGATGACA GTGAAATAGA CAGGTATATA CTTAATGAAA CAGAAGCTCA GATAAAAGCT 
GAACTGTGGA TGAGAGAAAA CGCAGATTAT TTGAAGGAAC AAAAAGAAAA AGAAGCAAGG 
ATAGCAAAAG AGAAGGAGCT TGGGATTTAT AAGGAACAAA AGCCAAAGAA ATCTGCAAAA 
AAACGGGAGC CAATTCAAGC CAGCACAGCA GGAGAAGCAA TTGAAAAGAT GTTAGAACAG 
AAGAAAATTT CAAGTAAAAT TAATTATAAT GTGCTAAGGG ACCTGAACAG CAAAGGAAGT 
AGCACACCAA AGAAAGAGGA TGACAGCACT GATGACAGCA GCAACACCAA GAAGCTGTCA 
AGAAGAAAAT CTATTGCCAG TAGGAATTTA GCCAACTCTG TAAACACTGT GGGAAAAAGA 
TTAAGACCCT TGATTTCTTC ACAACTAGCT AAAAAGGCTG CAACTGAAGA GGTTACCTTT 
TCAGACTTAA AGCCAGAAGT GCAGGAGTCG GGAAAAGCAG AGAGTGTGCT GGTGGAGAGT 
GGACCAGTGT CCTATAACCA TGACGATGAT GCTGAAGAAG AAGAAGTAGA AGAGGAGGAT 
GATCATTGCA TGAGTGCCCT TCAGTTAATG GGAGGAAATG ATTATGGCTG TGATATGGAT 
GACGATGATG GCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019335500.1
CDS	100..1797
Translation	MAVSKHLTRGRKMTHVIAACLYLVCRTEGTPHMLLDLSDLLQVNVYVLGKTFLLLARELCINAPAIDPCLYIPRFAHMLEFGDKNHEVSMTALRLLQRMKRDWMHTGRRPSGLCGAALLVAARMHDFRRTVKEVIRVVKVCESTLRKRLTEFEDTPTSQLTIDEFMKIDLEGECDPPSFTAGQRKLKLQQLEKELSKKLEDYEGEISIYQDEIEIELENSRPKAKGVFANYTKDDSIEDNTSSIFGEEEVEDEELEAAANHLNKDFCNEILDKDRLKTNEDRECRNGNAPTVRPPALESLLGPLPTAASLGITESIKVCISTKERVPNENTGDGELDLSGIDDSEIDRYILNETEAQIKAELWMRENADYLKEQKEKEARIAKEKELGIYKEQKPKKSAKKREPIQASTAGEAIEKMLEQKKISSKINYNVLRDLNSKGSSTPKKEDDSTDDSSNTKKLSRRKSIASRNLANSVNTVGKRLRPLISSQLAKKAATEEVTFSDLKPEVQESGKAESVLVESGPVSYNHDDDAEEEEVEEEDDHCMSALQLMGGNDYGCDMDDDDGY

Target ORF information:

RefSeq Version	XM_019479955.1
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis BRF1, RNA polymerase III transcription initiation factor subunit (BRF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019479955.1

ORF Insert Sequence:

ATGGCAGTGA GCAAGCACCT CACCCGTGGA AGAAAGATGA CTCACGTAAT TGCTGCGTGT 
CTCTACTTGG TGTGTAGAAC AGAAGGAACT CCTCACATGT TACTTGACCT CAGTGACCTC 
CTTCAGGTGA ATGTCTATGT GCTGGGAAAA ACTTTTCTTC TGTTGGCCAG AGAGCTCTGC 
ATAAATGCTC CAGCTATAGA TCCTTGTTTA TATATTCCTC GTTTTGCACA CATGCTAGAA 
TTTGGAGATA AGAACCATGA GGTATCGATG ACGGCTCTAA GGTTATTACA GAGGATGAAG 
AGAGACTGGA TGCACACAGG ACGTCGACCG TCAGGGTTGT GTGGAGCAGC CCTTCTAGTG 
GCAGCAAGAA TGCATGATTT CCGACGTACT GTTAAGGAGG TCATCAGAGT GGTCAAGGTT 
TGTGAGTCTA CGTTACGGAA AAGGCTTACA GAATTTGAAG ATACCCCAAC AAGTCAGCTC 
ACGATAGATG AATTTATGAA GATTGATTTG GAAGGGGAAT GTGATCCTCC TTCGTTTACA 
GCTGGTCAAA GAAAACTAAA GCTGCAGCAG CTCGAGAAGG AGCTGTCAAA AAAGCTTGAA 
GATTATGAAG GTGAAATATC AATTTACCAA GATGAAATAG AGATTGAATT GGAAAATAGT 
AGGCCAAAGG CAAAAGGAGT ATTTGCAAAT TACACTAAAG ACGATTCTAT AGAAGATAAT 
ACTTCTAGCA TATTTGGAGA GGAGGAGGTA GAAGATGAAG AACTGGAAGC AGCTGCTAAT 
CATTTAAACA AGGACTTTTG TAATGAAATT CTTGACAAAG ACAGATTAAA GACAAATGAA 
GATAGAGAAT GCAGAAATGG GAACGCACCT ACTGTAAGAC CACCTGCGCT GGAATCCTTG 
CTTGGCCCAC TCCCTACTGC AGCTAGCCTT GGAATAACAG AGTCCATAAA AGTTTGTATC 
TCCACAAAGG AGCGAGTGCC CAATGAAAAT ACAGGAGATG GTGAATTAGA TTTGAGTGGA 
ATTGATGACA GTGAAATAGA CAGGTATATA CTTAATGAAA CAGAAGCTCA GATAAAAGCT 
GAACTGTGGA TGAGAGAAAA CGCAGATTAT TTGAAGGAAC AAAAAGAAAA AGAAGCAAGG 
ATAGCAAAAG AGAAGGAGCT TGGGATTTAT AAGGAACAAA AGCCAAAGAA ATCTGCAAAA 
AAACGGGAGC CAATTCAAGC CAGCACAGCA GGAGAAGCAA TTGAAAAGAT GTTAGAACAG 
AAGAAAATTT CAAGTAAAAT TAATTATAAT GTGCTAAGGG ACCTGAACAG CAAAGGAAGT 
AGCACACCAA AGAAAGAGGA TGACAGCACT GATGACAGCA GCAACACCAA GAAGCTGTCA 
AGAAGAAAAT CTATTGCCAG TAGGAATTTA GCCAACTCTG TAAACACTGT GGGAAAAAGA 
TTAAGACCCT TGATTTCTTC ACAACTAGCT AAAAAGGCTG CAACTGAAGA GGTTACCTTT 
TCAGACTTAA AGCCAGAAGT GCAGGAGTCG GGAAAAGCAG AGAGTGTGCT GGTGGAGAGT 
GGACCAGTGT CCTATAACCA TGACGATGAT GCTGAAGAAG AAGAAGTAGA AGAGGAGGAT 
GATCATTGCA TGAGTGCCCT TCAGTTAATG GGAGGAAATG ATTATGGCTG TGATATGGAT 
GACGATGATG GCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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BRF1 cDNA ORF clone, Alligator mississippiensis(American alligator)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム