USP53 cDNA ORF clone, Alligator mississippiensis(American alligator)

Gene
Clones
gRNAs

Summary

Gene Symbol	USP53
Entrez Gene ID	102561603
Full Name	ubiquitin specific peptidase 53
Gene Type	protein-coding
Organism	Alligator mississippiensis(American alligator)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006259661.3	XP_006259723.2	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
XM_019494884.1	XP_019350429.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X2

Pathways from BioSystems
Gene Ontology

The following USP53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAl47771	XM_006259661.3 Latest version!	Alligator mississippiensis ubiquitin specific peptidase 53 (USP53), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAl47772	XM_019494884.1 Latest version!	Alligator mississippiensis ubiquitin specific peptidase 53 (USP53), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

USP53 ( XM_006259661.3 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_006259723.2 Alligator mississippiensis ubiquitin specific peptidase 53 (USP53), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAl47771
Clone ID Related Accession (Same CDS sequence)	XM_006259661.3
Accession Version	XM_006259661.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017707836.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 9, 2016 this sequence version replaced XM_006259661.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTGGG CAAAATTTTT ACGGAAACCT GGAGGTGGTA ACCTTGGAAA AGTGTACCAG 
CCTGGAAGTA TGTTATCTTT GGCCCCTACC AAAGGTTTAT TAAATGAACC AGGACAAAAC 
AGTTGCTTCC TTAACAGTGC TGTACAGGTT TTATGGCAGC TTGACATATT CCGACGGAGT 
TTAAGAGGTT TAACAGGGCA TGTTTGTCAA GGAGATGCCT GCATATTTTG TGCATTAAAG 
ACTATATTTG CACAGTTCCA ACACAGTCGG GAAAAAGCAC TCCCATCAGA TAATATGAGG 
CATGCCCTGG CTGAAAGCTT TAAAGATGAA CAGCGTTTTC AGCTTGGATT GATGGATGAT 
GCAGCAGAAT GCTTTGAAAA TATACTTGAG AGGATTCATT TCCATATAGT TCCAAGTAGT 
GAAACGGATA TGTGCACATC TAAATCCTGC ATTGCTCATC AGAAGTTTGC TATGACACTA 
TATGAACAGT GCGTGTGCCG CAGTTGTGGG GCATCTTCAG ACCCCTTGCC TTTCACAGAA 
TTTGTGCGTT ACATTTCTAC AACTGCCTTG TGCAATGAAG TTGATAGAAT GATGGAAAGG 
CATGAACGCC TCAAGCCAGA AATGTTTGCA GAATTGCTAC AGGCAGCAAA TACCACTGAT 
GACTTCAGAA AATGTCCTAG TAACTGTGGT CAAAAAATAA AAATCCGTCG TGTTCTCATG 
AACTGTCCCG AGATTGTCAC AATTGGTTTA GTCTGGGATT CGGAACATTC TGACTTGACA 
GAAGATGTTA TGCGGAATCT GGCAACACAG CTTTATCTTC CTGGGTTGTT CTATAGGGTT 
ACAGATGAGC AGGCTAAAAA TGCTGAGCTT TATCTTGTGG GGATGATTTG CTACGCAAGC 
CGACATTACT GTGCCTTTGC CTTTCACACT AAGAGCTGCA AGTGGGTCCT GTTTGATGAT 
GCTAATGTGA AAGAGGTTGG AACAAAATGG AAAGATGTGG TCTCCAAGTG CATTCGATGT 
CACTTTCAGC CATTGTTGCT ATTTTATGCT AACCCAGATG GTACAGCTGT ATCCACAGAA 
GATGCACCTA GACAGATTAT TCATTCTCAC TACAAATCGG CAGCAAGAAA TGGGGAAGGC 
TTAGGTTTTG AAAAACCAGC TGTTCCAAAG TTGGATCACA CAAGAGAGAA TGGATGTGGA 
GAGCCTCCAA ATCAAAAGGG CAATAAAAAA TTTCAGCCTG AAAACGTCAG TTTCAGCAGA 
GCTCATGTTC AGGCTAGTGG TGGTAAGGGA CCATTTAAGT TAGGGCACCA AGATCAAAAG 
GACAAGTTCA AGGATATATC CAGAGAATGT GCTCAGAAGG CAGTTGATAT GAGAAACGTT 
CAGTCCTCCC TAAGGAAAGA TGCAGACAGA GGACCAAAAA AAGACTCTGG GAGACAAAGA 
GATCTAATTG GAGAAGACCG CTGTCACATT AAGTCAGGAT CGCCTCCTGT TGGGAATGGA 
TTTAGACAAT ATGCTGACCA GCGCATTTAT AGTAGCCAGG GAAGAGGACC TTACAAACAT 
GAAAGTATAC CACATGAACC CAGACTGCCT TCACAGGTAC TTGGGTCAAA TAAAACAGAA 
GCTTTTCTTG CTGTGGAAAA AGGGATGAAC AGATCTAGGA TTGACAATAC CACTGGCTAT 
GAAACAGATA GCAGCCAAGA TTCTAGGGAC AAGGGAAGTG TTGTCAGCAG CAGTAGAAGC 
AAAAGCAAAG CTTGGAAGCC TATGCGAGAG ACATTAAATG TGGACAGCAT TTTCAGCGAG 
ACTGAAAAAA AGCAACATAG TCCAAGACAT AAGGCAAATC AAAGCAATAA AGCTAAACAG 
GAAAAGGAGC AGAGCTTTAA TAAGTGGCCA AAAGAAAATC AAACCCAGAA GAGTTTAATG 
ACCATTTATG AAGATGAAAC AAAACAGGAG ACGGGGAGTC GAAGCTCTCT GGATTCTGAT 
GGAAAAGGGA ATCTGGAGAA AGGAAAAGGA TTTACAGAGA GAAAAGTCCA TGGTGACAAC 
TGGCAAATAC AAAGAACTGA ATCTGGATAT GAAAGCTGTG ATCATATCAG TAATGGATCT 
GCCACTCTGG ATTCGCCTGT CATTGAAGGA ACCAACCCAG TGGATATCCG AGGTGTTAAA 
GAAACTGTTT CCTTCAGTGA CCTGACTCCA TCAGCCAAAA AACCTGAAAA TATGTTGCAG 
TCTACCTCTC AGCAGAACAG GAACTATTTT GAGGACTTGA GAAGAGACCA GAGGAGTACA 
GAAGTTAATT ACAGACCTCA TAATAATTTC CCATCAGAAT CGCAGGCACA CAGTCCTCCA 
ATGAGAAGGA CTGAGATACC TGAGAGTAAT TGGAAATTTC TTCCTTCATC GACTCTACAG 
CCAACTCATA AGGACAATGT TAAGCGAGAG ACTAGAATCA ATAAGCTATA TGAACAAAGT 
TCTTCAGAGC AGCTTAATTC AGATAAACTT GAGAAGACAG CCATGCAAGT GTGTTTTGCT 
GACGGCTTAC CCCATTCTTG TAATACTGAA AATGAACCTC TCTGTGGAAA GAGGCTGAAT 
AGCAGTGAAT TTCTCTTACC TTCTCAGCTG CAGGCTCCTA GTACTAGAAG TGCTACATCA 
AAAGCAGGGC TGTCATCTTT TGCACCCCAG AGCACATTGG AGCAATGCAA TGTGATGCCA 
TTTTCATCTG GTGTACGTAC AGTTCAGTCA TTTGGTAGGA CTGATCATCT TTGGGTCCCA 
GAAGCTGTTT ACCAAAATCT TCCACCTCCT TTACCTCCAA AGAAATATGC TCGGAACTTT 
TTGTCAGGAA CAGAAAGTAA TGAACCTCCA TGTGATGTAA AGTCAACAGG ATTCATGCAT 
GTTAATCCTT CAGATTTTCA GAGGCAAAGT ACAGGTACAG CTTCAACTGC ACCAGAAGCA 
GGCGTATGTA CAAATGAAGA CAGACTTAAA GAAGCATTTC AAGGAAAGGA GCCTTCTGTT 
CACAAACCAG ATTTTTCAAC TGGCCCATCA GTTAATGATT TTCAGACTCT GTCTGCTAAA 
CTTTTAAGTA AAGGATCATG CCACACAGGA CCAAGTGCTA ATTCTCCAGA TGCAAATGAT 
AGTGTATCTC TAACTACATA TTTTTCAGTA GATAGCTGTA TGACTGATAC ATACAGGATG 
AAATATCACC AGAGACCCAA ACTGTATTTC GCAGATCCTG GTAGTTTCCA CAAAGAATTG 
GGCACTGCTT ACCACAGTAC TGTTGATTCC AGTCATCCTG CATCCGAACA GAGATACAGA 
CCGCATATGG AAAGTATACA CTGCAATAGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006259723.2
CDS	678..4010
Translation	MAWAKFLRKPGGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRGLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNMRHALAESFKDEQRFQLGLMDDAAECFENILERIHFHIVPSSETDMCTSKSCIAHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVDRMMERHERLKPEMFAELLQAANTTDDFRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEDVMRNLATQLYLPGLFYRVTDEQAKNAELYLVGMICYASRHYCAFAFHTKSCKWVLFDDANVKEVGTKWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDAPRQIIHSHYKSAARNGEGLGFEKPAVPKLDHTRENGCGEPPNQKGNKKFQPENVSFSRAHVQASGGKGPFKLGHQDQKDKFKDISRECAQKAVDMRNVQSSLRKDADRGPKKDSGRQRDLIGEDRCHIKSGSPPVGNGFRQYADQRIYSSQGRGPYKHESIPHEPRLPSQVLGSNKTEAFLAVEKGMNRSRIDNTTGYETDSSQDSRDKGSVVSSSRSKSKAWKPMRETLNVDSIFSETEKKQHSPRHKANQSNKAKQEKEQSFNKWPKENQTQKSLMTIYEDETKQETGSRSSLDSDGKGNLEKGKGFTERKVHGDNWQIQRTESGYESCDHISNGSATLDSPVIEGTNPVDIRGVKETVSFSDLTPSAKKPENMLQSTSQQNRNYFEDLRRDQRSTEVNYRPHNNFPSESQAHSPPMRRTEIPESNWKFLPSSTLQPTHKDNVKRETRINKLYEQSSSEQLNSDKLEKTAMQVCFADGLPHSCNTENEPLCGKRLNSSEFLLPSQLQAPSTRSATSKAGLSSFAPQSTLEQCNVMPFSSGVRTVQSFGRTDHLWVPEAVYQNLPPPLPPKKYARNFLSGTESNEPPCDVKSTGFMHVNPSDFQRQSTGTASTAPEAGVCTNEDRLKEAFQGKEPSVHKPDFSTGPSVNDFQTLSAKLLSKGSCHTGPSANSPDANDSVSLTTYFSVDSCMTDTYRMKYHQRPKLYFADPGSFHKELGTAYHSTVDSSHPASEQRYRPHMESIHCNR

Target ORF information:

RefSeq Version	XM_006259661.3
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis ubiquitin specific peptidase 53 (USP53), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006259661.3

ORF Insert Sequence:

ATGGCCTGGG CAAAATTTTT ACGGAAACCT GGAGGTGGTA ACCTTGGAAA AGTGTACCAG 
CCTGGAAGTA TGTTATCTTT GGCCCCTACC AAAGGTTTAT TAAATGAACC AGGACAAAAC 
AGTTGCTTCC TTAACAGTGC TGTACAGGTT TTATGGCAGC TTGACATATT CCGACGGAGT 
TTAAGAGGTT TAACAGGGCA TGTTTGTCAA GGAGATGCCT GCATATTTTG TGCATTAAAG 
ACTATATTTG CACAGTTCCA ACACAGTCGG GAAAAAGCAC TCCCATCAGA TAATATGAGG 
CATGCCCTGG CTGAAAGCTT TAAAGATGAA CAGCGTTTTC AGCTTGGATT GATGGATGAT 
GCAGCAGAAT GCTTTGAAAA TATACTTGAG AGGATTCATT TCCATATAGT TCCAAGTAGT 
GAAACGGATA TGTGCACATC TAAATCCTGC ATTGCTCATC AGAAGTTTGC TATGACACTA 
TATGAACAGT GCGTGTGCCG CAGTTGTGGG GCATCTTCAG ACCCCTTGCC TTTCACAGAA 
TTTGTGCGTT ACATTTCTAC AACTGCCTTG TGCAATGAAG TTGATAGAAT GATGGAAAGG 
CATGAACGCC TCAAGCCAGA AATGTTTGCA GAATTGCTAC AGGCAGCAAA TACCACTGAT 
GACTTCAGAA AATGTCCTAG TAACTGTGGT CAAAAAATAA AAATCCGTCG TGTTCTCATG 
AACTGTCCCG AGATTGTCAC AATTGGTTTA GTCTGGGATT CGGAACATTC TGACTTGACA 
GAAGATGTTA TGCGGAATCT GGCAACACAG CTTTATCTTC CTGGGTTGTT CTATAGGGTT 
ACAGATGAGC AGGCTAAAAA TGCTGAGCTT TATCTTGTGG GGATGATTTG CTACGCAAGC 
CGACATTACT GTGCCTTTGC CTTTCACACT AAGAGCTGCA AGTGGGTCCT GTTTGATGAT 
GCTAATGTGA AAGAGGTTGG AACAAAATGG AAAGATGTGG TCTCCAAGTG CATTCGATGT 
CACTTTCAGC CATTGTTGCT ATTTTATGCT AACCCAGATG GTACAGCTGT ATCCACAGAA 
GATGCACCTA GACAGATTAT TCATTCTCAC TACAAATCGG CAGCAAGAAA TGGGGAAGGC 
TTAGGTTTTG AAAAACCAGC TGTTCCAAAG TTGGATCACA CAAGAGAGAA TGGATGTGGA 
GAGCCTCCAA ATCAAAAGGG CAATAAAAAA TTTCAGCCTG AAAACGTCAG TTTCAGCAGA 
GCTCATGTTC AGGCTAGTGG TGGTAAGGGA CCATTTAAGT TAGGGCACCA AGATCAAAAG 
GACAAGTTCA AGGATATATC CAGAGAATGT GCTCAGAAGG CAGTTGATAT GAGAAACGTT 
CAGTCCTCCC TAAGGAAAGA TGCAGACAGA GGACCAAAAA AAGACTCTGG GAGACAAAGA 
GATCTAATTG GAGAAGACCG CTGTCACATT AAGTCAGGAT CGCCTCCTGT TGGGAATGGA 
TTTAGACAAT ATGCTGACCA GCGCATTTAT AGTAGCCAGG GAAGAGGACC TTACAAACAT 
GAAAGTATAC CACATGAACC CAGACTGCCT TCACAGGTAC TTGGGTCAAA TAAAACAGAA 
GCTTTTCTTG CTGTGGAAAA AGGGATGAAC AGATCTAGGA TTGACAATAC CACTGGCTAT 
GAAACAGATA GCAGCCAAGA TTCTAGGGAC AAGGGAAGTG TTGTCAGCAG CAGTAGAAGC 
AAAAGCAAAG CTTGGAAGCC TATGCGAGAG ACATTAAATG TGGACAGCAT TTTCAGCGAG 
ACTGAAAAAA AGCAACATAG TCCAAGACAT AAGGCAAATC AAAGCAATAA AGCTAAACAG 
GAAAAGGAGC AGAGCTTTAA TAAGTGGCCA AAAGAAAATC AAACCCAGAA GAGTTTAATG 
ACCATTTATG AAGATGAAAC AAAACAGGAG ACGGGGAGTC GAAGCTCTCT GGATTCTGAT 
GGAAAAGGGA ATCTGGAGAA AGGAAAAGGA TTTACAGAGA GAAAAGTCCA TGGTGACAAC 
TGGCAAATAC AAAGAACTGA ATCTGGATAT GAAAGCTGTG ATCATATCAG TAATGGATCT 
GCCACTCTGG ATTCGCCTGT CATTGAAGGA ACCAACCCAG TGGATATCCG AGGTGTTAAA 
GAAACTGTTT CCTTCAGTGA CCTGACTCCA TCAGCCAAAA AACCTGAAAA TATGTTGCAG 
TCTACCTCTC AGCAGAACAG GAACTATTTT GAGGACTTGA GAAGAGACCA GAGGAGTACA 
GAAGTTAATT ACAGACCTCA TAATAATTTC CCATCAGAAT CGCAGGCACA CAGTCCTCCA 
ATGAGAAGGA CTGAGATACC TGAGAGTAAT TGGAAATTTC TTCCTTCATC GACTCTACAG 
CCAACTCATA AGGACAATGT TAAGCGAGAG ACTAGAATCA ATAAGCTATA TGAACAAAGT 
TCTTCAGAGC AGCTTAATTC AGATAAACTT GAGAAGACAG CCATGCAAGT GTGTTTTGCT 
GACGGCTTAC CCCATTCTTG TAATACTGAA AATGAACCTC TCTGTGGAAA GAGGCTGAAT 
AGCAGTGAAT TTCTCTTACC TTCTCAGCTG CAGGCTCCTA GTACTAGAAG TGCTACATCA 
AAAGCAGGGC TGTCATCTTT TGCACCCCAG AGCACATTGG AGCAATGCAA TGTGATGCCA 
TTTTCATCTG GTGTACGTAC AGTTCAGTCA TTTGGTAGGA CTGATCATCT TTGGGTCCCA 
GAAGCTGTTT ACCAAAATCT TCCACCTCCT TTACCTCCAA AGAAATATGC TCGGAACTTT 
TTGTCAGGAA CAGAAAGTAA TGAACCTCCA TGTGATGTAA AGTCAACAGG ATTCATGCAT 
GTTAATCCTT CAGATTTTCA GAGGCAAAGT ACAGGTACAG CTTCAACTGC ACCAGAAGCA 
GGCGTATGTA CAAATGAAGA CAGACTTAAA GAAGCATTTC AAGGAAAGGA GCCTTCTGTT 
CACAAACCAG ATTTTTCAAC TGGCCCATCA GTTAATGATT TTCAGACTCT GTCTGCTAAA 
CTTTTAAGTA AAGGATCATG CCACACAGGA CCAAGTGCTA ATTCTCCAGA TGCAAATGAT 
AGTGTATCTC TAACTACATA TTTTTCAGTA GATAGCTGTA TGACTGATAC ATACAGGATG 
AAATATCACC AGAGACCCAA ACTGTATTTC GCAGATCCTG GTAGTTTCCA CAAAGAATTG 
GGCACTGCTT ACCACAGTAC TGTTGATTCC AGTCATCCTG CATCCGAACA GAGATACAGA 
CCGCATATGG AAAGTATACA CTGCAATAGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( XM_019494884.1 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_019350429.1 Alligator mississippiensis ubiquitin specific peptidase 53 (USP53), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAl47772
Clone ID Related Accession (Same CDS sequence)	XM_019494884.1
Accession Version	XM_019494884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017707836.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTGGG CAAAATTTTT ACGGAAACCT GGAGGTGGTA ACCTTGGAAA AGTGTACCAG 
CCTGGAAGTA TGTTATCTTT GGCCCCTACC AAAGGTTTAT TAAATGAACC AGGACAAAAC 
AGTTGCTTCC TTAACAGTGC TGTACAGGTT TTATGGCAGC TTGACATATT CCGACGGAGT 
TTAAGAGGTT TAACAGGGCA TGTTTGTCAA GGAGATGCCT GCATATTTTG TGCATTAAAG 
ACTATATTTG CACAGTTCCA ACACAGTCGG GAAAAAGCAC TCCCATCAGA TAATATGAGG 
CATGCCCTGG CTGAAAGCTT TAAAGATGAA CAGCGTTTTC AGCTTGGATT GATGGATGAT 
GCAGCAGAAT GCTTTGAAAA TATACTTGAG AGGATTCATT TCCATATAGT TCCAAGTAGT 
GAAACGGATA TGTGCACATC TAAATCCTGC ATTGCTCATC AGAAGTTTGC TATGACACTA 
TATGAACAGT GCGTGTGCCG CAGTTGTGGG GCATCTTCAG ACCCCTTGCC TTTCACAGAA 
TTTGTGCGTT ACATTTCTAC AACTGCCTTG TGCAATGAAG TTGATAGAAT GATGGAAAGG 
CATGAACGCC TCAAGCCAGA AATGTTTGCA GAATTGCTAC AGGCAGCAAA TACCACTGAT 
GACTTCAGAA AATGTCCTAG TAACTGTGGT CAAAAAATAA AAATCCGTCG TGTTCTCATG 
AACTGTCCCG AGATTGTCAC AATTGGTTTA GTCTGGGATT CGGAACATTC TGACTTGACA 
GAAGATGTTA TGCGGAATCT GGCAACACAG CTTTATCTTC CTGGGTTGTT CTATAGGGTT 
ACAGATGAGC AGGCTAAAAA TGCTGAGCTT TATCTTGTGG GGATGATTTG CTACGCAAGC 
CGACATTACT GTGCCTTTGC CTTTCACACT AAGAGCTGCA AGTGGGTCCT GTTTGATGAT 
GCTAATGTGA AAGAGGTTGG AACAAAATGG AAAGATGTGG TCTCCAAGTG CATTCGATGT 
CACTTTCAGC CATTGTTGCT ATTTTATGCT AACCCAGATG GTACAGCTGT ATCCACAGAA 
GATGCACCTA GACAGATTAT TCATTCTCAC TACAAATCGG CAGCAAGAAA TGGGGAAGGC 
TTAGGTTTTG AAAAACCAGC TGTTCCAAAG TTGGATCACA CAAGAGAGAA TGGATGTGGA 
GAGCCTCCAA ATCAAAAGGG CAATAAAAAA TTTCAGCCTG AAAACGTCAG TTTCAGCAGA 
GCTCATGTTC AGGCTAGTGG TGTTAAGTTA GGGCACCAAG ATCAAAAGGA CAAGTTCAAG 
GATATATCCA GAGAATGTGC TCAGAAGGCA GTTGATATGA GAAACGTTCA GTCCTCCCTA 
AGGAAAGATG CAGACAGAGG ACCAAAAAAA GACTCTGGGA GACAAAGAGA TCTAATTGGA 
GAAGACCGCT GTCACATTAA GTCAGGATCG CCTCCTGTTG GGAATGGATT TAGACAATAT 
GCTGACCAGC GCATTTATAG TAGCCAGGGA AGAGGACCTT ACAAACATGA AAGTATACCA 
CATGAACCCA GACTGCCTTC ACAGGTACTT GGGTCAAATA AAACAGAAGC TTTTCTTGCT 
GTGGAAAAAG GGATGAACAG ATCTAGGATT GACAATACCA CTGGCTATGA AACAGATAGC 
AGCCAAGATT CTAGGGACAA GGGAAGTGTT GTCAGCAGCA GTAGAAGCAA AAGCAAAGCT 
TGGAAGCCTA TGCGAGAGAC ATTAAATGTG GACAGCATTT TCAGCGAGAC TGAAAAAAAG 
CAACATAGTC CAAGACATAA GGCAAATCAA AGCAATAAAG CTAAACAGGA AAAGGAGCAG 
AGCTTTAATA AGTGGCCAAA AGAAAATCAA ACCCAGAAGA GTTTAATGAC CATTTATGAA 
GATGAAACAA AACAGGAGAC GGGGAGTCGA AGCTCTCTGG ATTCTGATGG AAAAGGGAAT 
CTGGAGAAAG GAAAAGGATT TACAGAGAGA AAAGTCCATG GTGACAACTG GCAAATACAA 
AGAACTGAAT CTGGATATGA AAGCTGTGAT CATATCAGTA ATGGATCTGC CACTCTGGAT 
TCGCCTGTCA TTGAAGGAAC CAACCCAGTG GATATCCGAG GTGTTAAAGA AACTGTTTCC 
TTCAGTGACC TGACTCCATC AGCCAAAAAA CCTGAAAATA TGTTGCAGTC TACCTCTCAG 
CAGAACAGGA ACTATTTTGA GGACTTGAGA AGAGACCAGA GGAGTACAGA AGTTAATTAC 
AGACCTCATA ATAATTTCCC ATCAGAATCG CAGGCACACA GTCCTCCAAT GAGAAGGACT 
GAGATACCTG AGAGTAATTG GAAATTTCTT CCTTCATCGA CTCTACAGCC AACTCATAAG 
GACAATGTTA AGCGAGAGAC TAGAATCAAT AAGCTATATG AACAAAGTTC TTCAGAGCAG 
CTTAATTCAG ATAAACTTGA GAAGACAGCC ATGCAAGTGT GTTTTGCTGA CGGCTTACCC 
CATTCTTGTA ATACTGAAAA TGAACCTCTC TGTGGAAAGA GGCTGAATAG CAGTGAATTT 
CTCTTACCTT CTCAGCTGCA GGCTCCTAGT ACTAGAAGTG CTACATCAAA AGCAGGGCTG 
TCATCTTTTG CACCCCAGAG CACATTGGAG CAATGCAATG TGATGCCATT TTCATCTGGT 
GTACGTACAG TTCAGTCATT TGGTAGGACT GATCATCTTT GGGTCCCAGA AGCTGTTTAC 
CAAAATCTTC CACCTCCTTT ACCTCCAAAG AAATATGCTC GGAACTTTTT GTCAGGAACA 
GAAAGTAATG AACCTCCATG TGATGTAAAG TCAACAGGAT TCATGCATGT TAATCCTTCA 
GATTTTCAGA GGCAAAGTAC AGGTACAGCT TCAACTGCAC CAGAAGCAGG CGTATGTACA 
AATGAAGACA GACTTAAAGA AGCATTTCAA GGAAAGGAGC CTTCTGTTCA CAAACCAGAT 
TTTTCAACTG GCCCATCAGT TAATGATTTT CAGACTCTGT CTGCTAAACT TTTAAGTAAA 
GGATCATGCC ACACAGGACC AAGTGCTAAT TCTCCAGATG CAAATGATAG TGTATCTCTA 
ACTACATATT TTTCAGTAGA TAGCTGTATG ACTGATACAT ACAGGATGAA ATATCACCAG 
AGACCCAAAC TGTATTTCGC AGATCCTGGT AGTTTCCACA AAGAATTGGG CACTGCTTAC 
CACAGTACTG TTGATTCCAG TCATCCTGCA TCCGAACAGA GATACAGACC GCATATGGAA 
AGTATACACT GCAATAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019350429.1
CDS	678..3998
Translation	MAWAKFLRKPGGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRGLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNMRHALAESFKDEQRFQLGLMDDAAECFENILERIHFHIVPSSETDMCTSKSCIAHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVDRMMERHERLKPEMFAELLQAANTTDDFRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEDVMRNLATQLYLPGLFYRVTDEQAKNAELYLVGMICYASRHYCAFAFHTKSCKWVLFDDANVKEVGTKWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDAPRQIIHSHYKSAARNGEGLGFEKPAVPKLDHTRENGCGEPPNQKGNKKFQPENVSFSRAHVQASGVKLGHQDQKDKFKDISRECAQKAVDMRNVQSSLRKDADRGPKKDSGRQRDLIGEDRCHIKSGSPPVGNGFRQYADQRIYSSQGRGPYKHESIPHEPRLPSQVLGSNKTEAFLAVEKGMNRSRIDNTTGYETDSSQDSRDKGSVVSSSRSKSKAWKPMRETLNVDSIFSETEKKQHSPRHKANQSNKAKQEKEQSFNKWPKENQTQKSLMTIYEDETKQETGSRSSLDSDGKGNLEKGKGFTERKVHGDNWQIQRTESGYESCDHISNGSATLDSPVIEGTNPVDIRGVKETVSFSDLTPSAKKPENMLQSTSQQNRNYFEDLRRDQRSTEVNYRPHNNFPSESQAHSPPMRRTEIPESNWKFLPSSTLQPTHKDNVKRETRINKLYEQSSSEQLNSDKLEKTAMQVCFADGLPHSCNTENEPLCGKRLNSSEFLLPSQLQAPSTRSATSKAGLSSFAPQSTLEQCNVMPFSSGVRTVQSFGRTDHLWVPEAVYQNLPPPLPPKKYARNFLSGTESNEPPCDVKSTGFMHVNPSDFQRQSTGTASTAPEAGVCTNEDRLKEAFQGKEPSVHKPDFSTGPSVNDFQTLSAKLLSKGSCHTGPSANSPDANDSVSLTTYFSVDSCMTDTYRMKYHQRPKLYFADPGSFHKELGTAYHSTVDSSHPASEQRYRPHMESIHCNR

Target ORF information:

RefSeq Version	XM_019494884.1
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis ubiquitin specific peptidase 53 (USP53), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019494884.1

ORF Insert Sequence:

ATGGCCTGGG CAAAATTTTT ACGGAAACCT GGAGGTGGTA ACCTTGGAAA AGTGTACCAG 
CCTGGAAGTA TGTTATCTTT GGCCCCTACC AAAGGTTTAT TAAATGAACC AGGACAAAAC 
AGTTGCTTCC TTAACAGTGC TGTACAGGTT TTATGGCAGC TTGACATATT CCGACGGAGT 
TTAAGAGGTT TAACAGGGCA TGTTTGTCAA GGAGATGCCT GCATATTTTG TGCATTAAAG 
ACTATATTTG CACAGTTCCA ACACAGTCGG GAAAAAGCAC TCCCATCAGA TAATATGAGG 
CATGCCCTGG CTGAAAGCTT TAAAGATGAA CAGCGTTTTC AGCTTGGATT GATGGATGAT 
GCAGCAGAAT GCTTTGAAAA TATACTTGAG AGGATTCATT TCCATATAGT TCCAAGTAGT 
GAAACGGATA TGTGCACATC TAAATCCTGC ATTGCTCATC AGAAGTTTGC TATGACACTA 
TATGAACAGT GCGTGTGCCG CAGTTGTGGG GCATCTTCAG ACCCCTTGCC TTTCACAGAA 
TTTGTGCGTT ACATTTCTAC AACTGCCTTG TGCAATGAAG TTGATAGAAT GATGGAAAGG 
CATGAACGCC TCAAGCCAGA AATGTTTGCA GAATTGCTAC AGGCAGCAAA TACCACTGAT 
GACTTCAGAA AATGTCCTAG TAACTGTGGT CAAAAAATAA AAATCCGTCG TGTTCTCATG 
AACTGTCCCG AGATTGTCAC AATTGGTTTA GTCTGGGATT CGGAACATTC TGACTTGACA 
GAAGATGTTA TGCGGAATCT GGCAACACAG CTTTATCTTC CTGGGTTGTT CTATAGGGTT 
ACAGATGAGC AGGCTAAAAA TGCTGAGCTT TATCTTGTGG GGATGATTTG CTACGCAAGC 
CGACATTACT GTGCCTTTGC CTTTCACACT AAGAGCTGCA AGTGGGTCCT GTTTGATGAT 
GCTAATGTGA AAGAGGTTGG AACAAAATGG AAAGATGTGG TCTCCAAGTG CATTCGATGT 
CACTTTCAGC CATTGTTGCT ATTTTATGCT AACCCAGATG GTACAGCTGT ATCCACAGAA 
GATGCACCTA GACAGATTAT TCATTCTCAC TACAAATCGG CAGCAAGAAA TGGGGAAGGC 
TTAGGTTTTG AAAAACCAGC TGTTCCAAAG TTGGATCACA CAAGAGAGAA TGGATGTGGA 
GAGCCTCCAA ATCAAAAGGG CAATAAAAAA TTTCAGCCTG AAAACGTCAG TTTCAGCAGA 
GCTCATGTTC AGGCTAGTGG TGTTAAGTTA GGGCACCAAG ATCAAAAGGA CAAGTTCAAG 
GATATATCCA GAGAATGTGC TCAGAAGGCA GTTGATATGA GAAACGTTCA GTCCTCCCTA 
AGGAAAGATG CAGACAGAGG ACCAAAAAAA GACTCTGGGA GACAAAGAGA TCTAATTGGA 
GAAGACCGCT GTCACATTAA GTCAGGATCG CCTCCTGTTG GGAATGGATT TAGACAATAT 
GCTGACCAGC GCATTTATAG TAGCCAGGGA AGAGGACCTT ACAAACATGA AAGTATACCA 
CATGAACCCA GACTGCCTTC ACAGGTACTT GGGTCAAATA AAACAGAAGC TTTTCTTGCT 
GTGGAAAAAG GGATGAACAG ATCTAGGATT GACAATACCA CTGGCTATGA AACAGATAGC 
AGCCAAGATT CTAGGGACAA GGGAAGTGTT GTCAGCAGCA GTAGAAGCAA AAGCAAAGCT 
TGGAAGCCTA TGCGAGAGAC ATTAAATGTG GACAGCATTT TCAGCGAGAC TGAAAAAAAG 
CAACATAGTC CAAGACATAA GGCAAATCAA AGCAATAAAG CTAAACAGGA AAAGGAGCAG 
AGCTTTAATA AGTGGCCAAA AGAAAATCAA ACCCAGAAGA GTTTAATGAC CATTTATGAA 
GATGAAACAA AACAGGAGAC GGGGAGTCGA AGCTCTCTGG ATTCTGATGG AAAAGGGAAT 
CTGGAGAAAG GAAAAGGATT TACAGAGAGA AAAGTCCATG GTGACAACTG GCAAATACAA 
AGAACTGAAT CTGGATATGA AAGCTGTGAT CATATCAGTA ATGGATCTGC CACTCTGGAT 
TCGCCTGTCA TTGAAGGAAC CAACCCAGTG GATATCCGAG GTGTTAAAGA AACTGTTTCC 
TTCAGTGACC TGACTCCATC AGCCAAAAAA CCTGAAAATA TGTTGCAGTC TACCTCTCAG 
CAGAACAGGA ACTATTTTGA GGACTTGAGA AGAGACCAGA GGAGTACAGA AGTTAATTAC 
AGACCTCATA ATAATTTCCC ATCAGAATCG CAGGCACACA GTCCTCCAAT GAGAAGGACT 
GAGATACCTG AGAGTAATTG GAAATTTCTT CCTTCATCGA CTCTACAGCC AACTCATAAG 
GACAATGTTA AGCGAGAGAC TAGAATCAAT AAGCTATATG AACAAAGTTC TTCAGAGCAG 
CTTAATTCAG ATAAACTTGA GAAGACAGCC ATGCAAGTGT GTTTTGCTGA CGGCTTACCC 
CATTCTTGTA ATACTGAAAA TGAACCTCTC TGTGGAAAGA GGCTGAATAG CAGTGAATTT 
CTCTTACCTT CTCAGCTGCA GGCTCCTAGT ACTAGAAGTG CTACATCAAA AGCAGGGCTG 
TCATCTTTTG CACCCCAGAG CACATTGGAG CAATGCAATG TGATGCCATT TTCATCTGGT 
GTACGTACAG TTCAGTCATT TGGTAGGACT GATCATCTTT GGGTCCCAGA AGCTGTTTAC 
CAAAATCTTC CACCTCCTTT ACCTCCAAAG AAATATGCTC GGAACTTTTT GTCAGGAACA 
GAAAGTAATG AACCTCCATG TGATGTAAAG TCAACAGGAT TCATGCATGT TAATCCTTCA 
GATTTTCAGA GGCAAAGTAC AGGTACAGCT TCAACTGCAC CAGAAGCAGG CGTATGTACA 
AATGAAGACA GACTTAAAGA AGCATTTCAA GGAAAGGAGC CTTCTGTTCA CAAACCAGAT 
TTTTCAACTG GCCCATCAGT TAATGATTTT CAGACTCTGT CTGCTAAACT TTTAAGTAAA 
GGATCATGCC ACACAGGACC AAGTGCTAAT TCTCCAGATG CAAATGATAG TGTATCTCTA 
ACTACATATT TTTCAGTAGA TAGCTGTATG ACTGATACAT ACAGGATGAA ATATCACCAG 
AGACCCAAAC TGTATTTCGC AGATCCTGGT AGTTTCCACA AAGAATTGGG CACTGCTTAC 
CACAGTACTG TTGATTCCAG TCATCCTGCA TCCGAACAGA GATACAGACC GCATATGGAA 
AGTATACACT GCAATAGGTA G