LOC107344917 cDNA ORF clone, Acropora digitifera

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC107344917
Entrez Gene ID	107344917
Full Name	cartilage intermediate layer protein 1-like
Gene Type	protein-coding
Organism	Acropora digitifera

mRNA and Protein(s)

mRNA	Protein	Name
XM_015910602.1	XP_015766088.1	cartilage intermediate layer protein 1-like isoform X2
XM_015910601.1	XP_015766087.1	cartilage intermediate layer protein 2-like isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC107344917 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC107344917 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc73099	XM_015910601.1 Latest version!	Acropora digitifera cartilage intermediate layer protein 1-like (LOC107344917), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OAc73100	XM_015910602.1 Latest version!	Acropora digitifera cartilage intermediate layer protein 1-like (LOC107344917), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC107344917 ( XM_015910601.1 ) cDNA ORF clone, Acropora digitifera -> XP_015766087.1 Acropora digitifera cartilage intermediate layer protein 1-like (LOC107344917), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAc73099
Clone ID Related Accession (Same CDS sequence)	XM_015910601.1
Accession Version	XM_015910601.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-13
Organism	Acropora digitifera
Product	cartilage intermediate layer protein 2-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015441298.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acropora digitifera Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAA AACAAGCGTC CAAGAGCTCT GTCCATGAAA TGGTTGCTTT AGGAACTAGT 
ACGGAAGCTC TGATTTATGA AAGAACATCC AAAACGGAGC AACCGCCTGC TGATCCAGAT 
CTTGTGCCAG CGAAGTTCAC AGCCGTATCA AGTCGAAAGA GGACAGTCAT TTTGGCCACA 
GTGGTAATTG TCATCGTAAT TGCTGCAGTT TTTGTCGGGA TTTTTCTTTC CCAGGGCAAC 
AAAAACAAAG ATAAAGAGGA AAGCCAACAG GATAACGTGA AGTTAAGTCT TTCTGTAACG 
TTGACCTCCA GTTTGAATAA TGAGACCCAG CAGGCAGCTT CCTCGTCCAG TTACACAGAA 
GATAACGTGA AGCTAAGTCT TTCTGTAACG TTGACCTCCA GTTTGAATAA TCAGACTCAG 
CAGGCAGCTT CCTCGTCCAC TTTGTCATCT TCGAATGATC TACGAGCAAT TTCGGCCTGT 
AGCACTTGTA ATAAGATGTC TCCTGGAGAT ATTAGCACAC CAGCAACGAC ATTGTCCTCT 
CTCTCGTCAA TCATGCAGCT TGTTCCAAAC ACAGAAAATT ATATGGAGAT AAAACCTTCC 
GTGAAGATGA CGTCCACTTT GAGTAGCCAG ACTCAGTTAG AAGCGCCCAC ATCTATTTTG 
GCATCTTCCA TTGAGACACA AACAACTATT TCTGCTTGCA GCACTTGTAA TAAGCTGTCA 
CTGTCTCGTG AGGATATAAT AAGTTCACCA GCATCGACTT CATGCCCTCT CTCGTCAATC 
ATGGAGCTTG TTCCAAATAC AGATGCAAAG TGGTCTCGTT GGAGTCCGTG GAGTGATTGC 
ACCAAAACTT GTGGCAACGG AACCCGCTTT CGCACCAGAA CATGTGTTGC ACAAAATCAG 
CCACCAGTTT CAGCCCATAT TTCATGTGCA GGGAAACCTA ATCAAACAAG TATTTGTTCC 
CAGTGGAAAT GCCCAGATTG CAGGAAAACT TGCCTTAAAG GAACCCTGAA CGCTGCGTGT 
GATGCCTGTC TGTGTGATGA CCACGTGCTT ACTGGGCGCG TTCAAACTAC CAATAACGCG 
CCAATTGCAG GAGCCAAAGT TGCCTTCTCT GAGACACCGT ACAAAGCTCT AGCGAGAACA 
GATGAGAAGG GTTTCTTCAA AGCACTCAAT GTTTGTGCAG ATGCAAACCA GGAACTTCTC 
ATTAGCGCCG CTGGTTTTGT TCCAGTGAAA ACAGTGGCAA CTATCTCCAG CTCCAAAACG 
GCAAACGTTC AAGTGAAACT ACAGACGTCA GTACCTCCGA TAATAACCGT TCATCCACAG 
AGCAAGATGC GGTTTTCTGG TGACGCCGTA ACGTTTTGTT GTGACGGAGA GGGCAATCCC 
TCTCCTGAAA TCGAGTGGTT TAAAGACAAC AATATTATCG ATAAATCCAT CTACGGTTAC 
GACAAGCACC TTGAGCTTAG TAACGTGACT GGTCTAATGG GGAAATACCG ATGCCGAGTG 
GTGAACGACT ATGGAGCAGA GTTTTCTGAA GTTGCTGATT TGAACGTTTT CAGTTCAAGA 
AAAAAAGATT CGTGCAACCC GACTCCCATC AGTAAAAATG CAACACTTCC CACGGGCTGT 
GTTGTGAATG GGACAACAAG CAATATTATC GATGTGGGAG AGTGCCAACC GATTCCGTGT 
ATTCGAAACG ATTCCATTCT CTCAAAGAAA AATTGCCATG ATGAGAAGTT TTGTTGCTCG 
GTTGAAGAAG TAAGCGACGT TACCATAAGA TGTGGAAGCT CTGTCACATT TGCAATCTCA 
AAAGCATCTC GTTGTGGCTG TCAAAAATGC GAAGAACCTA AGTCACAAAT TAATGGAGTT 
CTCGTTGGTC GAAAAGGGCA GACCGAAAAA CCCATTGCCT ACAGCGATTT TACCTTTAAA 
GGCTTTTCAT ACAGGACAGA TGACAACGGC TTTTTCTCTT TTGAAGTGCC TACTGGAGCG 
AAAAGGGTGT CCGTGGTATT CAGGGACACT TATGACGACG AATTTGCAGA TTTGACGAAG 
GTTTTTCACG TCGAAGATGG TCAGACATTG TTTTCAAAAA TTATTCTCAA ACCGAAGCCT 
ATCCCACAGT CATTTAATAG CTCCGAACCA CACAAGGTTC AGCTCGGTGA AAGTCAAAAT 
GACACGGGGT TCGCTGAGAT GGTAATTCCA GGAGATGCTT TGTTGAAGGA TGATGGCAGC 
GTGTTTTCTG GTCAAGCAAA CCTAAGAATG GACGTCGTGG ATACCCGAAG CTTGTCTGAT 
ATGGTAGCAG CTCCAGCCGA TTTCACCACC GTTGACGAGG ATGGAGAAGA ACAAATGTTG 
GTCAGTTACG GAATGCTTAG TTTAGACTTT GAAGATGACA GTGGTAACAA GCTTTCTCCA 
TTTAGGCCCA TCAAACTCTA TCTGGACCCT GAAAAGCTAA ACATATCCGT TGATAGTAAT 
GGAAACACCA CAACAAAACT TTGGTGGCTG GACTCCACAA CAGGCAGATG GATCGAGGCA 
TCTGGTGTGC GAGTGGTAAA GAAAACCTCT ACTAGAGGCA AGCGATCTGT GACGCACTTC 
TTGCTAGAAA GTGAAATAGC TGCAGACATC TCGAGGCAAG GGAGACTCAA TATCGACTGG 
CCCACACCTC ATTACGCCGC AGTTCGAGTA AACGCTCCGA TGAGATCATC TATTAGAATA 
CTATGCGAAG AGCCTGGTTC AAGCCCGAAA AGGTATACGG GATATTTCGA AGAAACTGCC 
TGGTATTCAG AGATTGTCTG CATAAACCTT TGGGTTGATA GACTGTGTTA TATACAAGGA 
GAAAGTGACA ATTCCACGTT TTTGGAACCT TTCCCTCCTG ATAACTTTCC TCCGTCTGTT 
TCGGCAAAAG TTGTTTCAAA CGATATTCTC AAAGGCTCCA CCCGCCGAAT AAATTCCTTT 
AGATTTAAAG TAAAGACGTC AACTGATGGA CCAGTATTTT CTGGAACCAA TGGCAGAGAA 
GGTGTTAGGA AATGCCGCCA AGGTGCCGGC CTTCTACAGT TTGAATTCAA GTATCCCGTT 
TCTCCGAGCA TAAGTCTCAC AAGTACTCGT GAAACTGGTC CGAGAAACGT CAGCAACTGG 
TATCCTAATA ACGAAGCTTG TTTCATCAAA ATATTGACAA CAGGAGTAGG ACCGTTATTC 
CTCGTCACAA GTTATTGGCC AAACGAGACT TACAGATATG GAAACTCTGT TGAAACTGCA 
GAGAGGGTGC CCGGTAAAAA TGTTTTCGTT GCTTGTGTTG AGATTCGATG TCCAGGAAAC 
GTGTACCATC CAGATTCAAA GAAACCGCTT CCTGAGTGGA CGTACCTCCA CGTCACACAC 
TTGACTGGAA ACTGTAACCT AAAGGATGTT AAAAATAATC TGGCTAACCA AGAGAACCTT 
TATGGCGGCA ATAGAGGAAA CTGTTCTCAC TACTCTAAAA GTCATTCACC AGGATCCGCT 
GAAGCATGGC TGTGTGTTCC CCTCCCAAAT CGTGGCTTCG ACATCGATTT TGTTTATACT 
GGTAACAAAA ACGATCCTAC CATTGGTAAG AATCGGTGCT TAAACGGGAA CAACCGTTGG 
AAACGAGGGC AGCTTTCACA AGTAAACGAA AACGGACCTA CAATAAGGAT CCCTTGCAGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015766087.1
CDS	268..3930
Translation	MDSKQASKSSVHEMVALGTSTEALIYERTSKTEQPPADPDLVPAKFTAVSSRKRTVILATVVIVIVIAAVFVGIFLSQGNKNKDKEESQQDNVKLSLSVTLTSSLNNETQQAASSSSYTEDNVKLSLSVTLTSSLNNQTQQAASSSTLSSSNDLRAISACSTCNKMSPGDISTPATTLSSLSSIMQLVPNTENYMEIKPSVKMTSTLSSQTQLEAPTSILASSIETQTTISACSTCNKLSLSREDIISSPASTSCPLSSIMELVPNTDAKWSRWSPWSDCTKTCGNGTRFRTRTCVAQNQPPVSAHISCAGKPNQTSICSQWKCPDCRKTCLKGTLNAACDACLCDDHVLTGRVQTTNNAPIAGAKVAFSETPYKALARTDEKGFFKALNVCADANQELLISAAGFVPVKTVATISSSKTANVQVKLQTSVPPIITVHPQSKMRFSGDAVTFCCDGEGNPSPEIEWFKDNNIIDKSIYGYDKHLELSNVTGLMGKYRCRVVNDYGAEFSEVADLNVFSSRKKDSCNPTPISKNATLPTGCVVNGTTSNIIDVGECQPIPCIRNDSILSKKNCHDEKFCCSVEEVSDVTIRCGSSVTFAISKASRCGCQKCEEPKSQINGVLVGRKGQTEKPIAYSDFTFKGFSYRTDDNGFFSFEVPTGAKRVSVVFRDTYDDEFADLTKVFHVEDGQTLFSKIILKPKPIPQSFNSSEPHKVQLGESQNDTGFAEMVIPGDALLKDDGSVFSGQANLRMDVVDTRSLSDMVAAPADFTTVDEDGEEQMLVSYGMLSLDFEDDSGNKLSPFRPIKLYLDPEKLNISVDSNGNTTTKLWWLDSTTGRWIEASGVRVVKKTSTRGKRSVTHFLLESEIAADISRQGRLNIDWPTPHYAAVRVNAPMRSSIRILCEEPGSSPKRYTGYFEETAWYSEIVCINLWVDRLCYIQGESDNSTFLEPFPPDNFPPSVSAKVVSNDILKGSTRRINSFRFKVKTSTDGPVFSGTNGREGVRKCRQGAGLLQFEFKYPVSPSISLTSTRETGPRNVSNWYPNNEACFIKILTTGVGPLFLVTSYWPNETYRYGNSVETAERVPGKNVFVACVEIRCPGNVYHPDSKKPLPEWTYLHVTHLTGNCNLKDVKNNLANQENLYGGNRGNCSHYSKSHSPGSAEAWLCVPLPNRGFDIDFVYTGNKNDPTIGKNRCLNGNNRWKRGQLSQVNENGPTIRIPCS

Target ORF information:

RefSeq Version	XM_015910601.1
Organism	Acropora digitifera
Definition	Acropora digitifera cartilage intermediate layer protein 1-like (LOC107344917), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015910601.1

ORF Insert Sequence:

ATGGACTCAA AACAAGCGTC CAAGAGCTCT GTCCATGAAA TGGTTGCTTT AGGAACTAGT 
ACGGAAGCTC TGATTTATGA AAGAACATCC AAAACGGAGC AACCGCCTGC TGATCCAGAT 
CTTGTGCCAG CGAAGTTCAC AGCCGTATCA AGTCGAAAGA GGACAGTCAT TTTGGCCACA 
GTGGTAATTG TCATCGTAAT TGCTGCAGTT TTTGTCGGGA TTTTTCTTTC CCAGGGCAAC 
AAAAACAAAG ATAAAGAGGA AAGCCAACAG GATAACGTGA AGTTAAGTCT TTCTGTAACG 
TTGACCTCCA GTTTGAATAA TGAGACCCAG CAGGCAGCTT CCTCGTCCAG TTACACAGAA 
GATAACGTGA AGCTAAGTCT TTCTGTAACG TTGACCTCCA GTTTGAATAA TCAGACTCAG 
CAGGCAGCTT CCTCGTCCAC TTTGTCATCT TCGAATGATC TACGAGCAAT TTCGGCCTGT 
AGCACTTGTA ATAAGATGTC TCCTGGAGAT ATTAGCACAC CAGCAACGAC ATTGTCCTCT 
CTCTCGTCAA TCATGCAGCT TGTTCCAAAC ACAGAAAATT ATATGGAGAT AAAACCTTCC 
GTGAAGATGA CGTCCACTTT GAGTAGCCAG ACTCAGTTAG AAGCGCCCAC ATCTATTTTG 
GCATCTTCCA TTGAGACACA AACAACTATT TCTGCTTGCA GCACTTGTAA TAAGCTGTCA 
CTGTCTCGTG AGGATATAAT AAGTTCACCA GCATCGACTT CATGCCCTCT CTCGTCAATC 
ATGGAGCTTG TTCCAAATAC AGATGCAAAG TGGTCTCGTT GGAGTCCGTG GAGTGATTGC 
ACCAAAACTT GTGGCAACGG AACCCGCTTT CGCACCAGAA CATGTGTTGC ACAAAATCAG 
CCACCAGTTT CAGCCCATAT TTCATGTGCA GGGAAACCTA ATCAAACAAG TATTTGTTCC 
CAGTGGAAAT GCCCAGATTG CAGGAAAACT TGCCTTAAAG GAACCCTGAA CGCTGCGTGT 
GATGCCTGTC TGTGTGATGA CCACGTGCTT ACTGGGCGCG TTCAAACTAC CAATAACGCG 
CCAATTGCAG GAGCCAAAGT TGCCTTCTCT GAGACACCGT ACAAAGCTCT AGCGAGAACA 
GATGAGAAGG GTTTCTTCAA AGCACTCAAT GTTTGTGCAG ATGCAAACCA GGAACTTCTC 
ATTAGCGCCG CTGGTTTTGT TCCAGTGAAA ACAGTGGCAA CTATCTCCAG CTCCAAAACG 
GCAAACGTTC AAGTGAAACT ACAGACGTCA GTACCTCCGA TAATAACCGT TCATCCACAG 
AGCAAGATGC GGTTTTCTGG TGACGCCGTA ACGTTTTGTT GTGACGGAGA GGGCAATCCC 
TCTCCTGAAA TCGAGTGGTT TAAAGACAAC AATATTATCG ATAAATCCAT CTACGGTTAC 
GACAAGCACC TTGAGCTTAG TAACGTGACT GGTCTAATGG GGAAATACCG ATGCCGAGTG 
GTGAACGACT ATGGAGCAGA GTTTTCTGAA GTTGCTGATT TGAACGTTTT CAGTTCAAGA 
AAAAAAGATT CGTGCAACCC GACTCCCATC AGTAAAAATG CAACACTTCC CACGGGCTGT 
GTTGTGAATG GGACAACAAG CAATATTATC GATGTGGGAG AGTGCCAACC GATTCCGTGT 
ATTCGAAACG ATTCCATTCT CTCAAAGAAA AATTGCCATG ATGAGAAGTT TTGTTGCTCG 
GTTGAAGAAG TAAGCGACGT TACCATAAGA TGTGGAAGCT CTGTCACATT TGCAATCTCA 
AAAGCATCTC GTTGTGGCTG TCAAAAATGC GAAGAACCTA AGTCACAAAT TAATGGAGTT 
CTCGTTGGTC GAAAAGGGCA GACCGAAAAA CCCATTGCCT ACAGCGATTT TACCTTTAAA 
GGCTTTTCAT ACAGGACAGA TGACAACGGC TTTTTCTCTT TTGAAGTGCC TACTGGAGCG 
AAAAGGGTGT CCGTGGTATT CAGGGACACT TATGACGACG AATTTGCAGA TTTGACGAAG 
GTTTTTCACG TCGAAGATGG TCAGACATTG TTTTCAAAAA TTATTCTCAA ACCGAAGCCT 
ATCCCACAGT CATTTAATAG CTCCGAACCA CACAAGGTTC AGCTCGGTGA AAGTCAAAAT 
GACACGGGGT TCGCTGAGAT GGTAATTCCA GGAGATGCTT TGTTGAAGGA TGATGGCAGC 
GTGTTTTCTG GTCAAGCAAA CCTAAGAATG GACGTCGTGG ATACCCGAAG CTTGTCTGAT 
ATGGTAGCAG CTCCAGCCGA TTTCACCACC GTTGACGAGG ATGGAGAAGA ACAAATGTTG 
GTCAGTTACG GAATGCTTAG TTTAGACTTT GAAGATGACA GTGGTAACAA GCTTTCTCCA 
TTTAGGCCCA TCAAACTCTA TCTGGACCCT GAAAAGCTAA ACATATCCGT TGATAGTAAT 
GGAAACACCA CAACAAAACT TTGGTGGCTG GACTCCACAA CAGGCAGATG GATCGAGGCA 
TCTGGTGTGC GAGTGGTAAA GAAAACCTCT ACTAGAGGCA AGCGATCTGT GACGCACTTC 
TTGCTAGAAA GTGAAATAGC TGCAGACATC TCGAGGCAAG GGAGACTCAA TATCGACTGG 
CCCACACCTC ATTACGCCGC AGTTCGAGTA AACGCTCCGA TGAGATCATC TATTAGAATA 
CTATGCGAAG AGCCTGGTTC AAGCCCGAAA AGGTATACGG GATATTTCGA AGAAACTGCC 
TGGTATTCAG AGATTGTCTG CATAAACCTT TGGGTTGATA GACTGTGTTA TATACAAGGA 
GAAAGTGACA ATTCCACGTT TTTGGAACCT TTCCCTCCTG ATAACTTTCC TCCGTCTGTT 
TCGGCAAAAG TTGTTTCAAA CGATATTCTC AAAGGCTCCA CCCGCCGAAT AAATTCCTTT 
AGATTTAAAG TAAAGACGTC AACTGATGGA CCAGTATTTT CTGGAACCAA TGGCAGAGAA 
GGTGTTAGGA AATGCCGCCA AGGTGCCGGC CTTCTACAGT TTGAATTCAA GTATCCCGTT 
TCTCCGAGCA TAAGTCTCAC AAGTACTCGT GAAACTGGTC CGAGAAACGT CAGCAACTGG 
TATCCTAATA ACGAAGCTTG TTTCATCAAA ATATTGACAA CAGGAGTAGG ACCGTTATTC 
CTCGTCACAA GTTATTGGCC AAACGAGACT TACAGATATG GAAACTCTGT TGAAACTGCA 
GAGAGGGTGC CCGGTAAAAA TGTTTTCGTT GCTTGTGTTG AGATTCGATG TCCAGGAAAC 
GTGTACCATC CAGATTCAAA GAAACCGCTT CCTGAGTGGA CGTACCTCCA CGTCACACAC 
TTGACTGGAA ACTGTAACCT AAAGGATGTT AAAAATAATC TGGCTAACCA AGAGAACCTT 
TATGGCGGCA ATAGAGGAAA CTGTTCTCAC TACTCTAAAA GTCATTCACC AGGATCCGCT 
GAAGCATGGC TGTGTGTTCC CCTCCCAAAT CGTGGCTTCG ACATCGATTT TGTTTATACT 
GGTAACAAAA ACGATCCTAC CATTGGTAAG AATCGGTGCT TAAACGGGAA CAACCGTTGG 
AAACGAGGGC AGCTTTCACA AGTAAACGAA AACGGACCTA CAATAAGGAT CCCTTGCAGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107344917 ( XM_015910602.1 ) cDNA ORF clone, Acropora digitifera -> XP_015766088.1 Acropora digitifera cartilage intermediate layer protein 1-like (LOC107344917), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAc73100
Clone ID Related Accession (Same CDS sequence)	XM_015910602.1
Accession Version	XM_015910602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-13
Organism	Acropora digitifera
Product	cartilage intermediate layer protein 1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015441298.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acropora digitifera Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAA AACAAGCGTC CAAGAGCTCT GTCCATGAAA TGGTTGCTTT AGGAACTAGT 
ACGGAAGCTC TGATTTATGA AAGAACATCC AAAACGGAGC AACCGCCTGC TGATCCAGAT 
CTTGTGCCAG CGAAGTTCAC AGCCGTATCA AGTCGAAAGA GGACAGTCAT TTTGGCCACA 
GTGGTAATTG TCATCGTAAT TGCTGCAGTT TTTGTCGGGA TTTTTCTTTC CCAGGGCAAC 
AAAAACAAAG ATAAAGAGGA AAGCCAACAA GATAACGTGA AGCTAAGTCT TTCTGTAACG 
TTGACCTCCA GTTTGAATAA TCAGACTCAG CAGGCAGCTT CCTCGTCCAC TTTGTCATCT 
TCGAATGATC TACGAGCAAT TTCGGCCTGT AGCACTTGTA ATAAGATGTC TCCTGGAGAT 
ATTAGCACAC CAGCAACGAC ATTGTCCTCT CTCTCGTCAA TCATGCAGCT TGTTCCAAAC 
ACAGAAAATT ATATGGAGAT AAAACCTTCC GTGAAGATGA CGTCCACTTT GAGTAGCCAG 
ACTCAGTTAG AAGCGCCCAC ATCTATTTTG GCATCTTCCA TTGAGACACA AACAACTATT 
TCTGCTTGCA GCACTTGTAA TAAGCTGTCA CTGTCTCGTG AGGATATAAT AAGTTCACCA 
GCATCGACTT CATGCCCTCT CTCGTCAATC ATGGAGCTTG TTCCAAATAC AGATGCAAAG 
TGGTCTCGTT GGAGTCCGTG GAGTGATTGC ACCAAAACTT GTGGCAACGG AACCCGCTTT 
CGCACCAGAA CATGTGTTGC ACAAAATCAG CCACCAGTTT CAGCCCATAT TTCATGTGCA 
GGGAAACCTA ATCAAACAAG TATTTGTTCC CAGTGGAAAT GCCCAGATTG CAGGAAAACT 
TGCCTTAAAG GAACCCTGAA CGCTGCGTGT GATGCCTGTC TGTGTGATGA CCACGTGCTT 
ACTGGGCGCG TTCAAACTAC CAATAACGCG CCAATTGCAG GAGCCAAAGT TGCCTTCTCT 
GAGACACCGT ACAAAGCTCT AGCGAGAACA GATGAGAAGG GTTTCTTCAA AGCACTCAAT 
GTTTGTGCAG ATGCAAACCA GGAACTTCTC ATTAGCGCCG CTGGTTTTGT TCCAGTGAAA 
ACAGTGGCAA CTATCTCCAG CTCCAAAACG GCAAACGTTC AAGTGAAACT ACAGACGTCA 
GTACCTCCGA TAATAACCGT TCATCCACAG AGCAAGATGC GGTTTTCTGG TGACGCCGTA 
ACGTTTTGTT GTGACGGAGA GGGCAATCCC TCTCCTGAAA TCGAGTGGTT TAAAGACAAC 
AATATTATCG ATAAATCCAT CTACGGTTAC GACAAGCACC TTGAGCTTAG TAACGTGACT 
GGTCTAATGG GGAAATACCG ATGCCGAGTG GTGAACGACT ATGGAGCAGA GTTTTCTGAA 
GTTGCTGATT TGAACGTTTT CAGTTCAAGA AAAAAAGATT CGTGCAACCC GACTCCCATC 
AGTAAAAATG CAACACTTCC CACGGGCTGT GTTGTGAATG GGACAACAAG CAATATTATC 
GATGTGGGAG AGTGCCAACC GATTCCGTGT ATTCGAAACG ATTCCATTCT CTCAAAGAAA 
AATTGCCATG ATGAGAAGTT TTGTTGCTCG GTTGAAGAAG TAAGCGACGT TACCATAAGA 
TGTGGAAGCT CTGTCACATT TGCAATCTCA AAAGCATCTC GTTGTGGCTG TCAAAAATGC 
GAAGAACCTA AGTCACAAAT TAATGGAGTT CTCGTTGGTC GAAAAGGGCA GACCGAAAAA 
CCCATTGCCT ACAGCGATTT TACCTTTAAA GGCTTTTCAT ACAGGACAGA TGACAACGGC 
TTTTTCTCTT TTGAAGTGCC TACTGGAGCG AAAAGGGTGT CCGTGGTATT CAGGGACACT 
TATGACGACG AATTTGCAGA TTTGACGAAG GTTTTTCACG TCGAAGATGG TCAGACATTG 
TTTTCAAAAA TTATTCTCAA ACCGAAGCCT ATCCCACAGT CATTTAATAG CTCCGAACCA 
CACAAGGTTC AGCTCGGTGA AAGTCAAAAT GACACGGGGT TCGCTGAGAT GGTAATTCCA 
GGAGATGCTT TGTTGAAGGA TGATGGCAGC GTGTTTTCTG GTCAAGCAAA CCTAAGAATG 
GACGTCGTGG ATACCCGAAG CTTGTCTGAT ATGGTAGCAG CTCCAGCCGA TTTCACCACC 
GTTGACGAGG ATGGAGAAGA ACAAATGTTG GTCAGTTACG GAATGCTTAG TTTAGACTTT 
GAAGATGACA GTGGTAACAA GCTTTCTCCA TTTAGGCCCA TCAAACTCTA TCTGGACCCT 
GAAAAGCTAA ACATATCCGT TGATAGTAAT GGAAACACCA CAACAAAACT TTGGTGGCTG 
GACTCCACAA CAGGCAGATG GATCGAGGCA TCTGGTGTGC GAGTGGTAAA GAAAACCTCT 
ACTAGAGGCA AGCGATCTGT GACGCACTTC TTGCTAGAAA GTGAAATAGC TGCAGACATC 
TCGAGGCAAG GGAGACTCAA TATCGACTGG CCCACACCTC ATTACGCCGC AGTTCGAGTA 
AACGCTCCGA TGAGATCATC TATTAGAATA CTATGCGAAG AGCCTGGTTC AAGCCCGAAA 
AGGTATACGG GATATTTCGA AGAAACTGCC TGGTATTCAG AGATTGTCTG CATAAACCTT 
TGGGTTGATA GACTGTGTTA TATACAAGGA GAAAGTGACA ATTCCACGTT TTTGGAACCT 
TTCCCTCCTG ATAACTTTCC TCCGTCTGTT TCGGCAAAAG TTGTTTCAAA CGATATTCTC 
AAAGGCTCCA CCCGCCGAAT AAATTCCTTT AGATTTAAAG TAAAGACGTC AACTGATGGA 
CCAGTATTTT CTGGAACCAA TGGCAGAGAA GGTGTTAGGA AATGCCGCCA AGGTGCCGGC 
CTTCTACAGT TTGAATTCAA GTATCCCGTT TCTCCGAGCA TAAGTCTCAC AAGTACTCGT 
GAAACTGGTC CGAGAAACGT CAGCAACTGG TATCCTAATA ACGAAGCTTG TTTCATCAAA 
ATATTGACAA CAGGAGTAGG ACCGTTATTC CTCGTCACAA GTTATTGGCC AAACGAGACT 
TACAGATATG GAAACTCTGT TGAAACTGCA GAGAGGGTGC CCGGTAAAAA TGTTTTCGTT 
GCTTGTGTTG AGATTCGATG TCCAGGAAAC GTGTACCATC CAGATTCAAA GAAACCGCTT 
CCTGAGTGGA CGTACCTCCA CGTCACACAC TTGACTGGAA ACTGTAACCT AAAGGATGTT 
AAAAATAATC TGGCTAACCA AGAGAACCTT TATGGCGGCA ATAGAGGAAA CTGTTCTCAC 
TACTCTAAAA GTCATTCACC AGGATCCGCT GAAGCATGGC TGTGTGTTCC CCTCCCAAAT 
CGTGGCTTCG ACATCGATTT TGTTTATACT GGTAACAAAA ACGATCCTAC CATTGGTAAG 
AATCGGTGCT TAAACGGGAA CAACCGTTGG AAACGAGGGC AGCTTTCACA AGTAAACGAA 
AACGGACCTA CAATAAGGAT CCCTTGCAGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015766088.1
CDS	267..3839
Translation	MDSKQASKSSVHEMVALGTSTEALIYERTSKTEQPPADPDLVPAKFTAVSSRKRTVILATVVIVIVIAAVFVGIFLSQGNKNKDKEESQQDNVKLSLSVTLTSSLNNQTQQAASSSTLSSSNDLRAISACSTCNKMSPGDISTPATTLSSLSSIMQLVPNTENYMEIKPSVKMTSTLSSQTQLEAPTSILASSIETQTTISACSTCNKLSLSREDIISSPASTSCPLSSIMELVPNTDAKWSRWSPWSDCTKTCGNGTRFRTRTCVAQNQPPVSAHISCAGKPNQTSICSQWKCPDCRKTCLKGTLNAACDACLCDDHVLTGRVQTTNNAPIAGAKVAFSETPYKALARTDEKGFFKALNVCADANQELLISAAGFVPVKTVATISSSKTANVQVKLQTSVPPIITVHPQSKMRFSGDAVTFCCDGEGNPSPEIEWFKDNNIIDKSIYGYDKHLELSNVTGLMGKYRCRVVNDYGAEFSEVADLNVFSSRKKDSCNPTPISKNATLPTGCVVNGTTSNIIDVGECQPIPCIRNDSILSKKNCHDEKFCCSVEEVSDVTIRCGSSVTFAISKASRCGCQKCEEPKSQINGVLVGRKGQTEKPIAYSDFTFKGFSYRTDDNGFFSFEVPTGAKRVSVVFRDTYDDEFADLTKVFHVEDGQTLFSKIILKPKPIPQSFNSSEPHKVQLGESQNDTGFAEMVIPGDALLKDDGSVFSGQANLRMDVVDTRSLSDMVAAPADFTTVDEDGEEQMLVSYGMLSLDFEDDSGNKLSPFRPIKLYLDPEKLNISVDSNGNTTTKLWWLDSTTGRWIEASGVRVVKKTSTRGKRSVTHFLLESEIAADISRQGRLNIDWPTPHYAAVRVNAPMRSSIRILCEEPGSSPKRYTGYFEETAWYSEIVCINLWVDRLCYIQGESDNSTFLEPFPPDNFPPSVSAKVVSNDILKGSTRRINSFRFKVKTSTDGPVFSGTNGREGVRKCRQGAGLLQFEFKYPVSPSISLTSTRETGPRNVSNWYPNNEACFIKILTTGVGPLFLVTSYWPNETYRYGNSVETAERVPGKNVFVACVEIRCPGNVYHPDSKKPLPEWTYLHVTHLTGNCNLKDVKNNLANQENLYGGNRGNCSHYSKSHSPGSAEAWLCVPLPNRGFDIDFVYTGNKNDPTIGKNRCLNGNNRWKRGQLSQVNENGPTIRIPCS

Target ORF information:

RefSeq Version	XM_015910602.1
Organism	Acropora digitifera
Definition	Acropora digitifera cartilage intermediate layer protein 1-like (LOC107344917), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015910602.1

ORF Insert Sequence:

ATGGACTCAA AACAAGCGTC CAAGAGCTCT GTCCATGAAA TGGTTGCTTT AGGAACTAGT 
ACGGAAGCTC TGATTTATGA AAGAACATCC AAAACGGAGC AACCGCCTGC TGATCCAGAT 
CTTGTGCCAG CGAAGTTCAC AGCCGTATCA AGTCGAAAGA GGACAGTCAT TTTGGCCACA 
GTGGTAATTG TCATCGTAAT TGCTGCAGTT TTTGTCGGGA TTTTTCTTTC CCAGGGCAAC 
AAAAACAAAG ATAAAGAGGA AAGCCAACAA GATAACGTGA AGCTAAGTCT TTCTGTAACG 
TTGACCTCCA GTTTGAATAA TCAGACTCAG CAGGCAGCTT CCTCGTCCAC TTTGTCATCT 
TCGAATGATC TACGAGCAAT TTCGGCCTGT AGCACTTGTA ATAAGATGTC TCCTGGAGAT 
ATTAGCACAC CAGCAACGAC ATTGTCCTCT CTCTCGTCAA TCATGCAGCT TGTTCCAAAC 
ACAGAAAATT ATATGGAGAT AAAACCTTCC GTGAAGATGA CGTCCACTTT GAGTAGCCAG 
ACTCAGTTAG AAGCGCCCAC ATCTATTTTG GCATCTTCCA TTGAGACACA AACAACTATT 
TCTGCTTGCA GCACTTGTAA TAAGCTGTCA CTGTCTCGTG AGGATATAAT AAGTTCACCA 
GCATCGACTT CATGCCCTCT CTCGTCAATC ATGGAGCTTG TTCCAAATAC AGATGCAAAG 
TGGTCTCGTT GGAGTCCGTG GAGTGATTGC ACCAAAACTT GTGGCAACGG AACCCGCTTT 
CGCACCAGAA CATGTGTTGC ACAAAATCAG CCACCAGTTT CAGCCCATAT TTCATGTGCA 
GGGAAACCTA ATCAAACAAG TATTTGTTCC CAGTGGAAAT GCCCAGATTG CAGGAAAACT 
TGCCTTAAAG GAACCCTGAA CGCTGCGTGT GATGCCTGTC TGTGTGATGA CCACGTGCTT 
ACTGGGCGCG TTCAAACTAC CAATAACGCG CCAATTGCAG GAGCCAAAGT TGCCTTCTCT 
GAGACACCGT ACAAAGCTCT AGCGAGAACA GATGAGAAGG GTTTCTTCAA AGCACTCAAT 
GTTTGTGCAG ATGCAAACCA GGAACTTCTC ATTAGCGCCG CTGGTTTTGT TCCAGTGAAA 
ACAGTGGCAA CTATCTCCAG CTCCAAAACG GCAAACGTTC AAGTGAAACT ACAGACGTCA 
GTACCTCCGA TAATAACCGT TCATCCACAG AGCAAGATGC GGTTTTCTGG TGACGCCGTA 
ACGTTTTGTT GTGACGGAGA GGGCAATCCC TCTCCTGAAA TCGAGTGGTT TAAAGACAAC 
AATATTATCG ATAAATCCAT CTACGGTTAC GACAAGCACC TTGAGCTTAG TAACGTGACT 
GGTCTAATGG GGAAATACCG ATGCCGAGTG GTGAACGACT ATGGAGCAGA GTTTTCTGAA 
GTTGCTGATT TGAACGTTTT CAGTTCAAGA AAAAAAGATT CGTGCAACCC GACTCCCATC 
AGTAAAAATG CAACACTTCC CACGGGCTGT GTTGTGAATG GGACAACAAG CAATATTATC 
GATGTGGGAG AGTGCCAACC GATTCCGTGT ATTCGAAACG ATTCCATTCT CTCAAAGAAA 
AATTGCCATG ATGAGAAGTT TTGTTGCTCG GTTGAAGAAG TAAGCGACGT TACCATAAGA 
TGTGGAAGCT CTGTCACATT TGCAATCTCA AAAGCATCTC GTTGTGGCTG TCAAAAATGC 
GAAGAACCTA AGTCACAAAT TAATGGAGTT CTCGTTGGTC GAAAAGGGCA GACCGAAAAA 
CCCATTGCCT ACAGCGATTT TACCTTTAAA GGCTTTTCAT ACAGGACAGA TGACAACGGC 
TTTTTCTCTT TTGAAGTGCC TACTGGAGCG AAAAGGGTGT CCGTGGTATT CAGGGACACT 
TATGACGACG AATTTGCAGA TTTGACGAAG GTTTTTCACG TCGAAGATGG TCAGACATTG 
TTTTCAAAAA TTATTCTCAA ACCGAAGCCT ATCCCACAGT CATTTAATAG CTCCGAACCA 
CACAAGGTTC AGCTCGGTGA AAGTCAAAAT GACACGGGGT TCGCTGAGAT GGTAATTCCA 
GGAGATGCTT TGTTGAAGGA TGATGGCAGC GTGTTTTCTG GTCAAGCAAA CCTAAGAATG 
GACGTCGTGG ATACCCGAAG CTTGTCTGAT ATGGTAGCAG CTCCAGCCGA TTTCACCACC 
GTTGACGAGG ATGGAGAAGA ACAAATGTTG GTCAGTTACG GAATGCTTAG TTTAGACTTT 
GAAGATGACA GTGGTAACAA GCTTTCTCCA TTTAGGCCCA TCAAACTCTA TCTGGACCCT 
GAAAAGCTAA ACATATCCGT TGATAGTAAT GGAAACACCA CAACAAAACT TTGGTGGCTG 
GACTCCACAA CAGGCAGATG GATCGAGGCA TCTGGTGTGC GAGTGGTAAA GAAAACCTCT 
ACTAGAGGCA AGCGATCTGT GACGCACTTC TTGCTAGAAA GTGAAATAGC TGCAGACATC 
TCGAGGCAAG GGAGACTCAA TATCGACTGG CCCACACCTC ATTACGCCGC AGTTCGAGTA 
AACGCTCCGA TGAGATCATC TATTAGAATA CTATGCGAAG AGCCTGGTTC AAGCCCGAAA 
AGGTATACGG GATATTTCGA AGAAACTGCC TGGTATTCAG AGATTGTCTG CATAAACCTT 
TGGGTTGATA GACTGTGTTA TATACAAGGA GAAAGTGACA ATTCCACGTT TTTGGAACCT 
TTCCCTCCTG ATAACTTTCC TCCGTCTGTT TCGGCAAAAG TTGTTTCAAA CGATATTCTC 
AAAGGCTCCA CCCGCCGAAT AAATTCCTTT AGATTTAAAG TAAAGACGTC AACTGATGGA 
CCAGTATTTT CTGGAACCAA TGGCAGAGAA GGTGTTAGGA AATGCCGCCA AGGTGCCGGC 
CTTCTACAGT TTGAATTCAA GTATCCCGTT TCTCCGAGCA TAAGTCTCAC AAGTACTCGT 
GAAACTGGTC CGAGAAACGT CAGCAACTGG TATCCTAATA ACGAAGCTTG TTTCATCAAA 
ATATTGACAA CAGGAGTAGG ACCGTTATTC CTCGTCACAA GTTATTGGCC AAACGAGACT 
TACAGATATG GAAACTCTGT TGAAACTGCA GAGAGGGTGC CCGGTAAAAA TGTTTTCGTT 
GCTTGTGTTG AGATTCGATG TCCAGGAAAC GTGTACCATC CAGATTCAAA GAAACCGCTT 
CCTGAGTGGA CGTACCTCCA CGTCACACAC TTGACTGGAA ACTGTAACCT AAAGGATGTT 
AAAAATAATC TGGCTAACCA AGAGAACCTT TATGGCGGCA ATAGAGGAAA CTGTTCTCAC 
TACTCTAAAA GTCATTCACC AGGATCCGCT GAAGCATGGC TGTGTGTTCC CCTCCCAAAT 
CGTGGCTTCG ACATCGATTT TGTTTATACT GGTAACAAAA ACGATCCTAC CATTGGTAAG 
AATCGGTGCT TAAACGGGAA CAACCGTTGG AAACGAGGGC AGCTTTCACA AGTAAACGAA 
AACGGACCTA CAATAAGGAT CCCTTGCAGT TGA