phkb cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis)

Gene
Clones
gRNAs

Summary

Gene Symbol	phkb
Entrez Gene ID	110969139
Full Name	phosphorylase kinase regulatory subunit beta
Gene Type	protein-coding
Organism	Acanthochromis polyacanthus(spiny chromis)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022219178.1	XP_022074870.1	phosphorylase b kinase regulatory subunit beta

Pathways from BioSystems
Gene Ontology

The following phkb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the phkb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc181178	XM_022219178.1 Latest version!	Acanthochromis polyacanthus phosphorylase kinase regulatory subunit beta (phkb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

phkb ( XM_022219178.1 ) cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis) -> XP_022074870.1 Acanthochromis polyacanthus phosphorylase kinase regulatory subunit beta (phkb), mRNA.

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CloneID	OAc181178
Clone ID Related Accession (Same CDS sequence)	XM_022219178.1
Accession Version	XM_022219178.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-06
Organism	Acanthochromis polyacanthus(spiny chromis)
Product	phosphorylase b kinase regulatory subunit beta
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019031388.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthochromis polyacanthus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCAGA AAGATGCTTC CTCTGTTAAT CTCTCTGTTC TCTCTCCTTC ACAGGGTCCC 
ATAGAGAATG ACGTTGTGAT TCACGCGGCT CTGATAGCTG AAAGCCAGAG ATTGCAAGTA 
TTTTTGAACA CCTATGGCAT TCAGACCCAG ACACCCCAGC AGGTGGAACC CATCCAGATC 
TGGCCTCAGA AGGAACTGGT CAAGGCATAT CGTTTCCTTG CCATCAATAA GAAGCTGGGG 
TTGAGTGGGC GTCCAGATAG GCCAGTGGGG TGTATTGGGA CCTGCAAGAT TTATCGCATT 
CTGGGCAAGA CGGTGGTGTG CTACCCAATA GTGTTTGATC TAAGTGACTT TTACTTGTCC 
CAAGATGTTA TGCTGCTGAT TGATGACATT AAGAATGCCT TGCAGTTCAT CAAACAGTGC 
TGGAAGATGC AGGGGCGTCC TCTGTTCCTG GTTCTCATCC GCGAGGATAA CATCAAAGGG 
AGCCGCTTCA ACCCAGTCCT GGACATGCTG GCCGCGTTCA AGAAAGGCAA CATCGGCGGA 
GTCAAAGTTC ACGTTGACAG ACTCCAGACC CTGATATCTG GAGCAGTTGT GGAGCAGTTG 
GACTTCTTGC GAGTCAATGA GGCAGAGATC CCAGAATTCA AGAGCTTTGA GGAGCTTGAG 
ATGCCAAAGC ACTCTAAGGT GAAGAGACAG ACAAGCACAC CCAACGCCTC AGACCTGGAA 
CAGCAGCCAG AGATCAGTGT GGAGGAGTGG CAGCACAAGT CCACCCAGGA AATCATCCAG 
AAGTTCCATG ACTGCGACTG CTTGGCCAGT CAGGCTCAGC TTGCCAGCAT CCTGCTTAGG 
AGAGAAGGAC CAGACTTCCT TTCCAAAGAT GAGAACCTGA TGGATGAACT GGAGAGAATT 
TACAGAAGAG CCGGCAGCAG AAAGCTTTGG TCTGTTGTCC GTCTTGCTGC CAGTCTCTTA 
ACTAAGCTAG TGGACAGCCT TGCCCCCAGT ATTACTAGTG TTTTAGTACA TGGCAAGCAG 
GTAACCCTCG GCCTATTCGG CCATGAGGAG GAGGTGATCT CTAACCCACT GTCCCCAGGA 
GTGATCCAGG GCATCATCTA CAGCAAGTGC TCCCCTCACG GTGGGGAGAG GGAGGCTGTT 
TTACAGCAGG AACTGGTCAT CCACATCGGA TGGATTATCT CCAACAACCC TGAGCTGTTC 
AGTGGCATGC TCAAGATCAG AGTCGGGTGG ATCGTTCAGG CCATGAAACA TGAGCTAAAG 
ATCCGTGCAG GAGACATGCC ACCACAGGAC ATCTACCAAC TGTCCCCCAG CGACATCAAG 
CAGCTCCTGC TGGATGTCCT GCAGCCACAA CACACCGGCA GGTCTTGGCT GAACAGGCGT 
CAGGTCGACG GCTCTCTGAA CAGGACCCCG CTGGGCTTTT ATGATCGTGT GTGGCAGATC 
CTGGAGAGGA CCCCCAATGG TATCGTGGTG GCTGAGAATC ACCTCCCACA GCAACCATCA 
CTCTCTGACA TGACCATGTA TGAGATGAAC TTCTCCCTGC TGGTAGAGGA CACGCTGAAG 
AATATCGTCC TGCCTGAGTA CAGACAAATC ATAGTAGAGC TGTTGATGGT GGTGTCTATA 
GTGCTAGAGA GAAACCCAGA GCTTGAGTTC AGTGACAAGG TGGATCTGGA CGGACTGGTG 
AAGGAAGCCT TTAATGATTT CCAGAGAGAC CGCAGCCGCT TTGAAGGAAT GGAGAAACAG 
GACAACATGG AGGCATTCTA CAACACACCA CCGGTGGGAA AGAGAGGCAC CTCCAGCTAC 
CTGACCAAGG CTGTAATGAT CCTGCTGCTG CAGGGCGACG TCAAGCCCTG CAAGGATGAC 
CCGTGCTCTG TTAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022074870.1
CDS	805..2682
Translation	MLQKDASSVNLSVLSPSQGPIENDVVIHAALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQKELVKAYRFLAINKKLGLSGRPDRPVGCIGTCKIYRILGKTVVCYPIVFDLSDFYLSQDVMLLIDDIKNALQFIKQCWKMQGRPLFLVLIREDNIKGSRFNPVLDMLAAFKKGNIGGVKVHVDRLQTLISGAVVEQLDFLRVNEAEIPEFKSFEELEMPKHSKVKRQTSTPNASDLEQQPEISVEEWQHKSTQEIIQKFHDCDCLASQAQLASILLRREGPDFLSKDENLMDELERIYRRAGSRKLWSVVRLAASLLTKLVDSLAPSITSVLVHGKQVTLGLFGHEEEVISNPLSPGVIQGIIYSKCSPHGGEREAVLQQELVIHIGWIISNNPELFSGMLKIRVGWIVQAMKHELKIRAGDMPPQDIYQLSPSDIKQLLLDVLQPQHTGRSWLNRRQVDGSLNRTPLGFYDRVWQILERTPNGIVVAENHLPQQPSLSDMTMYEMNFSLLVEDTLKNIVLPEYRQIIVELLMVVSIVLERNPELEFSDKVDLDGLVKEAFNDFQRDRSRFEGMEKQDNMEAFYNTPPVGKRGTSSYLTKAVMILLLQGDVKPCKDDPCSVS

Target ORF information:

RefSeq Version	XM_022219178.1
Organism	Acanthochromis polyacanthus(spiny chromis)
Definition	Acanthochromis polyacanthus phosphorylase kinase regulatory subunit beta (phkb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022219178.1

ORF Insert Sequence:

ATGTTGCAGA AAGATGCTTC CTCTGTTAAT CTCTCTGTTC TCTCTCCTTC ACAGGGTCCC 
ATAGAGAATG ACGTTGTGAT TCACGCGGCT CTGATAGCTG AAAGCCAGAG ATTGCAAGTA 
TTTTTGAACA CCTATGGCAT TCAGACCCAG ACACCCCAGC AGGTGGAACC CATCCAGATC 
TGGCCTCAGA AGGAACTGGT CAAGGCATAT CGTTTCCTTG CCATCAATAA GAAGCTGGGG 
TTGAGTGGGC GTCCAGATAG GCCAGTGGGG TGTATTGGGA CCTGCAAGAT TTATCGCATT 
CTGGGCAAGA CGGTGGTGTG CTACCCAATA GTGTTTGATC TAAGTGACTT TTACTTGTCC 
CAAGATGTTA TGCTGCTGAT TGATGACATT AAGAATGCCT TGCAGTTCAT CAAACAGTGC 
TGGAAGATGC AGGGGCGTCC TCTGTTCCTG GTTCTCATCC GCGAGGATAA CATCAAAGGG 
AGCCGCTTCA ACCCAGTCCT GGACATGCTG GCCGCGTTCA AGAAAGGCAA CATCGGCGGA 
GTCAAAGTTC ACGTTGACAG ACTCCAGACC CTGATATCTG GAGCAGTTGT GGAGCAGTTG 
GACTTCTTGC GAGTCAATGA GGCAGAGATC CCAGAATTCA AGAGCTTTGA GGAGCTTGAG 
ATGCCAAAGC ACTCTAAGGT GAAGAGACAG ACAAGCACAC CCAACGCCTC AGACCTGGAA 
CAGCAGCCAG AGATCAGTGT GGAGGAGTGG CAGCACAAGT CCACCCAGGA AATCATCCAG 
AAGTTCCATG ACTGCGACTG CTTGGCCAGT CAGGCTCAGC TTGCCAGCAT CCTGCTTAGG 
AGAGAAGGAC CAGACTTCCT TTCCAAAGAT GAGAACCTGA TGGATGAACT GGAGAGAATT 
TACAGAAGAG CCGGCAGCAG AAAGCTTTGG TCTGTTGTCC GTCTTGCTGC CAGTCTCTTA 
ACTAAGCTAG TGGACAGCCT TGCCCCCAGT ATTACTAGTG TTTTAGTACA TGGCAAGCAG 
GTAACCCTCG GCCTATTCGG CCATGAGGAG GAGGTGATCT CTAACCCACT GTCCCCAGGA 
GTGATCCAGG GCATCATCTA CAGCAAGTGC TCCCCTCACG GTGGGGAGAG GGAGGCTGTT 
TTACAGCAGG AACTGGTCAT CCACATCGGA TGGATTATCT CCAACAACCC TGAGCTGTTC 
AGTGGCATGC TCAAGATCAG AGTCGGGTGG ATCGTTCAGG CCATGAAACA TGAGCTAAAG 
ATCCGTGCAG GAGACATGCC ACCACAGGAC ATCTACCAAC TGTCCCCCAG CGACATCAAG 
CAGCTCCTGC TGGATGTCCT GCAGCCACAA CACACCGGCA GGTCTTGGCT GAACAGGCGT 
CAGGTCGACG GCTCTCTGAA CAGGACCCCG CTGGGCTTTT ATGATCGTGT GTGGCAGATC 
CTGGAGAGGA CCCCCAATGG TATCGTGGTG GCTGAGAATC ACCTCCCACA GCAACCATCA 
CTCTCTGACA TGACCATGTA TGAGATGAAC TTCTCCCTGC TGGTAGAGGA CACGCTGAAG 
AATATCGTCC TGCCTGAGTA CAGACAAATC ATAGTAGAGC TGTTGATGGT GGTGTCTATA 
GTGCTAGAGA GAAACCCAGA GCTTGAGTTC AGTGACAAGG TGGATCTGGA CGGACTGGTG 
AAGGAAGCCT TTAATGATTT CCAGAGAGAC CGCAGCCGCT TTGAAGGAAT GGAGAAACAG 
GACAACATGG AGGCATTCTA CAACACACCA CCGGTGGGAA AGAGAGGCAC CTCCAGCTAC 
CTGACCAAGG CTGTAATGAT CCTGCTGCTG CAGGGCGACG TCAAGCCCTG CAAGGATGAC 
CCGTGCTCTG TTAGTTAG