tkfc cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis)

Gene
Clones
gRNAs

Summary

Gene Symbol	tkfc
Entrez Gene ID	110967840
Full Name	triokinase and FMN cyclase
Gene Type	protein-coding
Organism	Acanthochromis polyacanthus(spiny chromis)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022217562.1	XP_022073254.1	triokinase/FMN cyclase

Pathways from BioSystems
Gene Ontology

The following tkfc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tkfc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc179662	XM_022217562.1 Latest version!	Acanthochromis polyacanthus triokinase and FMN cyclase (tkfc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tkfc ( XM_022217562.1 ) cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis) -> XP_022073254.1 Acanthochromis polyacanthus triokinase and FMN cyclase (tkfc), mRNA.

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CloneID	OAc179662
Clone ID Related Accession (Same CDS sequence)	XM_022217562.1
Accession Version	XM_022217562.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-06
Organism	Acanthochromis polyacanthus(spiny chromis)
Product	triokinase/FMN cyclase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019031033.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthochromis polyacanthus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCTC AGAAGAAGCT GATAAACTCG GTGGACTCCT GTGTGGATGA AGCTCTCTGG 
GGTCTGGTTC GGGCCTCTGG GGGCCTCAGT CTTCTGACGG GCCACAGAGT CGTGGTTCGC 
TCAGACCTCC AGAGCCTGAA GGGGAAGGTG GCCCTGCTGT CTGGAGGAGG GTCCGGACAC 
GAGCCGGCAC ACGGAGGTTA CATCGGTGCT GGGATGCTGT CAGCAGCTGT AGCAGGAGGA 
GTGTTTGCGT CGCCGCCTCC TACCAGCATC CTGGCAGCCA TCTTGTCTCT ACACAACGCA 
GGAGCCTCTG GGGTCCTTCT GATCGTGAAG AACTACACCG GCGACCGACT CAACTTCGGC 
TTGGCAGCAG AACAGGCCCG TAACCATGGC GTCACTGTGG ACATGGTGAT AGTCGCCGAG 
GACTGTGCCT TCGACCGACC CAGTAAAGCC GGAAGACGAG GCCTGTGTGG AACCGTCTTC 
ATCCACAAGC TGGCAGGCGC CCTGGCAGAA GAAGGCTGCT CATTGGCCCA GATCGTCTCC 
ACGGTGACGG AGGTTTTGAA GGGGATTGGG ACTCTGGGGG TCAGTCTGTC TCCCTGCAGC 
GTTCCAGGAT GTCTGCCTTC ATTCGAGCTG CTGCCTGGAG ACATGGAGCT GGGACTGGGG 
ATCCACGGTG AACCTGGGAT CAAGAGGTCA AAGGTGTCTT CTGCAGATGA AGTGGTGAGG 
ACCATGATCG AGCACATGAC CAACCCCGAC AGCCAATCAC ATCTGCCGCT GCAGTCAGGA 
GACACCGTGG TTCTGTGTGT GAACAACCTG GGAGCTCTGT CCTGTCTGGA GATGTCCGTG 
GTCACCAGAG CCGCCATCAG CTGTCTGGAG GACCGTGGCG TGGTGGTTGC CAGGGCGATG 
TCCGGTTCGT TCATGACGTC GCTGGAGATG GCGGGCGTGT CGCTGACGCT GATGAAGGCC 
GACCAGGAAG TGCTGAGACT GTTTGATGCT CGGACTAGCG CCCCCGCCTG GCCGAACCTC 
AGCAGCATCA GTGTTAGTGG ACGAAGCTGC GTCACAGAAG CTCCGGTCAT GAGCACCAAA 
CCACAGGATG ACACCCAGAA TGAAGGCCCG CTGAGTCCTG TGATGCGTAA AGCGCTGGAG 
AAGATTTGTT CCACGCTGTT GGAGAAGCAG GAGGAACTGA ACTCTTTGGA CCGTGCTGCT 
GGGGATGGAG ATTGTGGTAA CACTCATGCT CAGGCAGCCA GAGCCATTCA GGAGTGGCTG 
CAGGGTCATG TGGTCCCTGG TTGCCCGGGG CGACTGCTGT CTGCGCTCGC CGGATTGGTG 
GAGGAGAAGA TGGGCGGGTC TTCAGGAGCG CTGTACAGTC TGTTCCTCAC CGCCGCCGCC 
GGTCACATGA CCGAGGGGCG GAGCAACGCT GCAGCCTGGG CCGCCGCAAT GCATGCTGGG 
ACACAGGCCA TGAGAAGGTA TGGAGGAGCT GATCCTGGAG ACAGAACGAT GCTGGATGCT 
TTATGTCCTG CAGTGGATGA ACTGATGAAG CTGAAAACGG CGCCCCCTGC TGAACATATG 
GCTGTACTGC AGAAAGCTGT GCAGAGAGCG GTCTCGGGGG CGGAGTCGAC CCGTGACCTC 
ACAGCCAGGG CGGGCCGAGC CAGCTACATC TCAGCCGACC GGGTCACCCT GCCGGACCCC 
GGCGCCGTGG CCGTGGCCGC CATCTTTACC GCCGTCATGG AGACACTGCA GACAGGAAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022073254.1
CDS	73..1815
Translation	MQPQKKLINSVDSCVDEALWGLVRASGGLSLLTGHRVVVRSDLQSLKGKVALLSGGGSGHEPAHGGYIGAGMLSAAVAGGVFASPPPTSILAAILSLHNAGASGVLLIVKNYTGDRLNFGLAAEQARNHGVTVDMVIVAEDCAFDRPSKAGRRGLCGTVFIHKLAGALAEEGCSLAQIVSTVTEVLKGIGTLGVSLSPCSVPGCLPSFELLPGDMELGLGIHGEPGIKRSKVSSADEVVRTMIEHMTNPDSQSHLPLQSGDTVVLCVNNLGALSCLEMSVVTRAAISCLEDRGVVVARAMSGSFMTSLEMAGVSLTLMKADQEVLRLFDARTSAPAWPNLSSISVSGRSCVTEAPVMSTKPQDDTQNEGPLSPVMRKALEKICSTLLEKQEELNSLDRAAGDGDCGNTHAQAARAIQEWLQGHVVPGCPGRLLSALAGLVEEKMGGSSGALYSLFLTAAAGHMTEGRSNAAAWAAAMHAGTQAMRRYGGADPGDRTMLDALCPAVDELMKLKTAPPAEHMAVLQKAVQRAVSGAESTRDLTARAGRASYISADRVTLPDPGAVAVAAIFTAVMETLQTGN

Target ORF information:

RefSeq Version	XM_022217562.1
Organism	Acanthochromis polyacanthus(spiny chromis)
Definition	Acanthochromis polyacanthus triokinase and FMN cyclase (tkfc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022217562.1

ORF Insert Sequence:

ATGCAGCCTC AGAAGAAGCT GATAAACTCG GTGGACTCCT GTGTGGATGA AGCTCTCTGG 
GGTCTGGTTC GGGCCTCTGG GGGCCTCAGT CTTCTGACGG GCCACAGAGT CGTGGTTCGC 
TCAGACCTCC AGAGCCTGAA GGGGAAGGTG GCCCTGCTGT CTGGAGGAGG GTCCGGACAC 
GAGCCGGCAC ACGGAGGTTA CATCGGTGCT GGGATGCTGT CAGCAGCTGT AGCAGGAGGA 
GTGTTTGCGT CGCCGCCTCC TACCAGCATC CTGGCAGCCA TCTTGTCTCT ACACAACGCA 
GGAGCCTCTG GGGTCCTTCT GATCGTGAAG AACTACACCG GCGACCGACT CAACTTCGGC 
TTGGCAGCAG AACAGGCCCG TAACCATGGC GTCACTGTGG ACATGGTGAT AGTCGCCGAG 
GACTGTGCCT TCGACCGACC CAGTAAAGCC GGAAGACGAG GCCTGTGTGG AACCGTCTTC 
ATCCACAAGC TGGCAGGCGC CCTGGCAGAA GAAGGCTGCT CATTGGCCCA GATCGTCTCC 
ACGGTGACGG AGGTTTTGAA GGGGATTGGG ACTCTGGGGG TCAGTCTGTC TCCCTGCAGC 
GTTCCAGGAT GTCTGCCTTC ATTCGAGCTG CTGCCTGGAG ACATGGAGCT GGGACTGGGG 
ATCCACGGTG AACCTGGGAT CAAGAGGTCA AAGGTGTCTT CTGCAGATGA AGTGGTGAGG 
ACCATGATCG AGCACATGAC CAACCCCGAC AGCCAATCAC ATCTGCCGCT GCAGTCAGGA 
GACACCGTGG TTCTGTGTGT GAACAACCTG GGAGCTCTGT CCTGTCTGGA GATGTCCGTG 
GTCACCAGAG CCGCCATCAG CTGTCTGGAG GACCGTGGCG TGGTGGTTGC CAGGGCGATG 
TCCGGTTCGT TCATGACGTC GCTGGAGATG GCGGGCGTGT CGCTGACGCT GATGAAGGCC 
GACCAGGAAG TGCTGAGACT GTTTGATGCT CGGACTAGCG CCCCCGCCTG GCCGAACCTC 
AGCAGCATCA GTGTTAGTGG ACGAAGCTGC GTCACAGAAG CTCCGGTCAT GAGCACCAAA 
CCACAGGATG ACACCCAGAA TGAAGGCCCG CTGAGTCCTG TGATGCGTAA AGCGCTGGAG 
AAGATTTGTT CCACGCTGTT GGAGAAGCAG GAGGAACTGA ACTCTTTGGA CCGTGCTGCT 
GGGGATGGAG ATTGTGGTAA CACTCATGCT CAGGCAGCCA GAGCCATTCA GGAGTGGCTG 
CAGGGTCATG TGGTCCCTGG TTGCCCGGGG CGACTGCTGT CTGCGCTCGC CGGATTGGTG 
GAGGAGAAGA TGGGCGGGTC TTCAGGAGCG CTGTACAGTC TGTTCCTCAC CGCCGCCGCC 
GGTCACATGA CCGAGGGGCG GAGCAACGCT GCAGCCTGGG CCGCCGCAAT GCATGCTGGG 
ACACAGGCCA TGAGAAGGTA TGGAGGAGCT GATCCTGGAG ACAGAACGAT GCTGGATGCT 
TTATGTCCTG CAGTGGATGA ACTGATGAAG CTGAAAACGG CGCCCCCTGC TGAACATATG 
GCTGTACTGC AGAAAGCTGT GCAGAGAGCG GTCTCGGGGG CGGAGTCGAC CCGTGACCTC 
ACAGCCAGGG CGGGCCGAGC CAGCTACATC TCAGCCGACC GGGTCACCCT GCCGGACCCC 
GGCGCCGTGG CCGTGGCCGC CATCTTTACC GCCGTCATGG AGACACTGCA GACAGGAAAT 
TAG