nisch cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis)

Gene
Clones
gRNAs

Summary

Gene Symbol	nisch
Entrez Gene ID	110966781
Full Name	nischarin
Gene Type	protein-coding
Organism	Acanthochromis polyacanthus(spiny chromis)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022216247.1	XP_022071939.1	nischarin

Pathways from BioSystems
Gene Ontology

The following nisch gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nisch cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc178651	XM_022216247.1 Latest version!	Acanthochromis polyacanthus nischarin (nisch), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nisch ( XM_022216247.1 ) cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis) -> XP_022071939.1 Acanthochromis polyacanthus nischarin (nisch), mRNA.

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CloneID	OAc178651
Clone ID Related Accession (Same CDS sequence)	XM_022216247.1
Accession Version	XM_022216247.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-06
Organism	Acanthochromis polyacanthus(spiny chromis)
Product	nischarin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019030792.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthochromis polyacanthus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGATTCTG CGCCGTTTTC AGAGGAGCTG TCAGAGAGGA AAGTGTGTGT CGTGGGCTCG 
GAGCTAGTGG AAAACTACAC GGTTTACATC ATCGACGTCA CGGACGGAAA GCACCGATGG 
ACAGTCAAAC ATCGCTACAG CGACTTCCAC GACCTCCATG AGAAGCTGGC GGCGGAGAAG 
AAGGTCGACC GCCGGCTTTT ACCCCCCAAG AAGATTCTGG GGAAGAACTC CAAGAGTCTG 
GTGGAGCGCC GGCAGAAGGA GCTGGAGCTT TACCTGCAGA CGCTGCTGCT ACAGTTCCCT 
CAGGCCACGC CCACAGCGCT CGCCTGCTTC CTGCACTTCC ACCTCTATGA GATCAACGGG 
ATCACAGCAG CTCTGGCTGA AGAGTTGTTC CATAAAGGTG AGTTCTGCTT ATATCTCTGT 
ATCTGCGGCT CCAGAGGTCC GGTGGGGAGC AGTGACATCC AGGAGAGCAG CCTCCCCTTT 
GACTTGTCCG TCTTCAAGTC CCTGCTGCAG ATCCAGATCA GTGACTGCAG CTCGGAGCAG 
ATTGAAGGTT TGTCGACTCT CAGGACGAGT TTGGAGACGA TGAACGTCCA CCAGAGTACA 
CAATCCATGA TGTCAGTTCT GGTCCCAGAG GCGGCGGAGT TCCCCCAGTG GGAACCTGAG 
GGGGTGGAGT CTGTGTGTCC TGTCACGGCA TTGGTGCCGG TGTGGTGTCA GCTGACCACC 
CTGGACCTGA GTCACAATGA CATCAGCTGC ATAGACAACT CTGTGAGGTT GATTCCAAAG 
GTGGAGTTCC TGGATCTGAG CTCCAATCAG CTGTCCTCAG TGGAGAACCT GCAGCACCTG 
TACAACCTGG TCCACGTGGA TCTGTCCTAC AACAACCTGC AGGTTCTGGA AGCTGCTCAC 
ACCCGTCTGG GGAACATTAA GACCCTGAAC CTGGCTGGAA ACCAGCTGCA GAACCTCAGT 
GGACTCTCCA AGCTCTACTC TCTGGTCAAC CTGGACCTCA GCCACAACCA GCTGGACCAG 
CTGGAGGAGA TCAGGAACAT TGGTTCTCTG CCCTGCCTGG AGAAGCTCAG CCTGGCCGGG 
AACCCCATGT GCATCATCCC CGACTACAGA ACCAAAGTCC TGGCCCAGTT TGGAGACCGA 
GCAGCTGAGG TGTGTCTGGA CAGCAGAGTG ACCACAGAGA AGGAGCTGGA CACCGTGGAG 
GTTCTCAAAG CCATCCAGAA GGCCAAAGAG GTCAAAGACC GGATGAGCAG CGGAGACAAG 
AAGATCAGTG AGGACACCAG ACCTCCTGCT GCTCCTGTCC TCTGCTCCTC CTCTTCCTCT 
CTGTCCTCCT CTGCTCCTCC TGCCTCCTCC TCCTCCTCCT GTCCGGCCCA GCAGGCTTCC 
AGCCAAGAAG TGACCAGCCC AGAAGAAGCC GCCGCCCCCC CCGCTGTTCT CCCCCCTGTA 
GACGCTACAA CCCCCCCTGC TAGCTTCAAC GCCCCCCACC TGTCTGCTGG CCCCGCCCAG 
GTCCAGCAGT CGTCCATCAG TCTGTCTGAA CTCTGTGGAG CTTCCACTGA TGTTCCTGCT 
GCTGATATCA CAGTCTGCTC TACCTGCTAC TCCTCCATCA CCTGTTCCTC CTGCAGCGCC 
CCCTGGTGTC CGCTGCTTCC TGCTCACCTG CTGCCGCTCT CCGCCTCCAA CCAGGACTTC 
GTTACCCAGC TGTCCCAGCG GCTCGGCTCC GCCCACAGGA AGCAGAGGCT GGAGGGGGAG 
GTGGAGGAGG AGAAGCCTGG ATCTGGAGAG CTTCTTCAGT CTGCTGGGGA TAGAACGGAG 
GTGGACGGTC TCAGCCCCGG CTCCAGGGAC GGCTACTTTG AGATGGGTCT GGGCGACTCT 
CTGGAGGAGG TGTTCTGCTC CTCCTCCATC GGCCTGCAGG TGGCGCCCAG CGAGGAGCAG 
CAGCAGCAGC AGCAGGAGGA AGAGGAGGAG GAAGAGGAGG AGGAGGAGGA GGTGAGGGTC 
AGCAGGGTTC TGTGGTGCTG CTGTGTTCTG GTGGAACATC AGGAGGAGCT GAAGCAGAGG 
GAGGCCTGTC TGGTTCTGAC CCACCACCTG CTGGGTCTGC TCTTCCTGTC ACCTGACTCC 
ACGGGGACCA ATCAGGACGC AGGTGAGCGG CTGGTGTTTG TTTGCAGGAT TTCTTTTTAT 
GTTCTCCTCA GCTGGAACTC CTCCCGTCCC CATGTGGAGG AGAACCATCC GTCCCCCCAC 
CTCCTGCCGG GTCTCTCCCC AGGACTGAAG CTCCTGGCCG GGCTGGGCGG GCAACAGCTG 
CTCGGCTACT TCCACAGATT CATCGCTCTG TCTGAGTCAG AGGATCTGAA GCAGGTTCTG 
TGGCTGTCGG TGGTTCTTTA TAAATCTCCA GAAGCTGAAC TCACCTGCTG CCTTCTGCTG 
TCCACCGACC ACATCTACTT CCTGCTGGAA GACTCCGCCT CCACGCTGAC GCATCACTCA 
GTCCTGGAGG TCTCTGATGC TGGAGATCCA GACTCCATCC TGGTCTGCTG TCTGTCCATC 
AGTCTGTCTG AGCTGCTGGC GGTGAACGTG GGTCTGTTCG ACCAGTACTT CAGAGTTGTA 
GGTAGATCAG CTGATCACAT CGTCTGCTGC CTCAGCAGGG ACAGTTACGG AACCGGAGTC 
TTCCTGCAGG AGCTGATGGC AGCACTGAGC CTCCAGCAGC AGCTTCCTCC TCCAGAGCCC 
TCCGACCAGG ACTTCTACTC CCAGTTCACC CACACCTGCT CAGGTAAGAT GCAGAACTAC 
GAGCTGGTCC ACAGCAGCCA GGTGAAGTTC ATCTACCCCA GCGAGGAGGA GATGGGAGAC 
CTGACCTTCA TCGTAGCAGA GAGGAAGACT CCGGCCAGCT GCTCCAGATC CTTCAATGTC 
CTGCTCTACC TGCTGGTGTT CCAGGTTCAG GTTCCGTCCA GTCAGAGTTC TGGTCCATCT 
GCTGTCCTCC AGCCCAGAAC TCTGATCCTC ACCAGCACCG ACGTCTTCCT GCTGGACGAG 
GACTACGTCA GCTACCCGCT GCCAGACTTC GCCAAAGAGC CTCCATCCAG GGAGCGCTAC 
CAGCTGCGTG AGGCCCGGCG GATCCGGGAC CTGGACCGAG TACTGCTGGG GTACCAGACC 
TACCCTCAGG CTCTGACGCT GGTGTTTGAC GACCTGCCCG GACCCGACCT GCTCTGCCAC 
CTCACCATGG ACCACTTTGC CGCCGGCGGG GAGGAGGCTC TGCCCAGAGG GGGCGGGGCC 
AGCAGGGGCG CAGAGGGTGA GGTCCAATGG TGCGTCTTCG TGCCTGGAGC CGACAGCAGA 
GAGAGGCTGA TCTCACTGCT GGCCCGCCAG TGGGAGGCGC AGTGCAGCCG CGAGCTGCCC 
GTGGAGCTCA CCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022071939.1
CDS	117..3494
Translation	MDSAPFSEELSERKVCVVGSELVENYTVYIIDVTDGKHRWTVKHRYSDFHDLHEKLAAEKKVDRRLLPPKKILGKNSKSLVERRQKELELYLQTLLLQFPQATPTALACFLHFHLYEINGITAALAEELFHKGEFCLYLCICGSRGPVGSSDIQESSLPFDLSVFKSLLQIQISDCSSEQIEGLSTLRTSLETMNVHQSTQSMMSVLVPEAAEFPQWEPEGVESVCPVTALVPVWCQLTTLDLSHNDISCIDNSVRLIPKVEFLDLSSNQLSSVENLQHLYNLVHVDLSYNNLQVLEAAHTRLGNIKTLNLAGNQLQNLSGLSKLYSLVNLDLSHNQLDQLEEIRNIGSLPCLEKLSLAGNPMCIIPDYRTKVLAQFGDRAAEVCLDSRVTTEKELDTVEVLKAIQKAKEVKDRMSSGDKKISEDTRPPAAPVLCSSSSSLSSSAPPASSSSSCPAQQASSQEVTSPEEAAAPPAVLPPVDATTPPASFNAPHLSAGPAQVQQSSISLSELCGASTDVPAADITVCSTCYSSITCSSCSAPWCPLLPAHLLPLSASNQDFVTQLSQRLGSAHRKQRLEGEVEEEKPGSGELLQSAGDRTEVDGLSPGSRDGYFEMGLGDSLEEVFCSSSIGLQVAPSEEQQQQQQEEEEEEEEEEEEVRVSRVLWCCCVLVEHQEELKQREACLVLTHHLLGLLFLSPDSTGTNQDAGERLVFVCRISFYVLLSWNSSRPHVEENHPSPHLLPGLSPGLKLLAGLGGQQLLGYFHRFIALSESEDLKQVLWLSVVLYKSPEAELTCCLLLSTDHIYFLLEDSASTLTHHSVLEVSDAGDPDSILVCCLSISLSELLAVNVGLFDQYFRVVGRSADHIVCCLSRDSYGTGVFLQELMAALSLQQQLPPPEPSDQDFYSQFTHTCSGKMQNYELVHSSQVKFIYPSEEEMGDLTFIVAERKTPASCSRSFNVLLYLLVFQVQVPSSQSSGPSAVLQPRTLILTSTDVFLLDEDYVSYPLPDFAKEPPSRERYQLREARRIRDLDRVLLGYQTYPQALTLVFDDLPGPDLLCHLTMDHFAAGGEEALPRGGGASRGAEGEVQWCVFVPGADSRERLISLLARQWEAQCSRELPVELTG

Target ORF information:

RefSeq Version	XM_022216247.1
Organism	Acanthochromis polyacanthus(spiny chromis)
Definition	Acanthochromis polyacanthus nischarin (nisch), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022216247.1

ORF Insert Sequence:

ATGGATTCTG CGCCGTTTTC AGAGGAGCTG TCAGAGAGGA AAGTGTGTGT CGTGGGCTCG 
GAGCTAGTGG AAAACTACAC GGTTTACATC ATCGACGTCA CGGACGGAAA GCACCGATGG 
ACAGTCAAAC ATCGCTACAG CGACTTCCAC GACCTCCATG AGAAGCTGGC GGCGGAGAAG 
AAGGTCGACC GCCGGCTTTT ACCCCCCAAG AAGATTCTGG GGAAGAACTC CAAGAGTCTG 
GTGGAGCGCC GGCAGAAGGA GCTGGAGCTT TACCTGCAGA CGCTGCTGCT ACAGTTCCCT 
CAGGCCACGC CCACAGCGCT CGCCTGCTTC CTGCACTTCC ACCTCTATGA GATCAACGGG 
ATCACAGCAG CTCTGGCTGA AGAGTTGTTC CATAAAGGTG AGTTCTGCTT ATATCTCTGT 
ATCTGCGGCT CCAGAGGTCC GGTGGGGAGC AGTGACATCC AGGAGAGCAG CCTCCCCTTT 
GACTTGTCCG TCTTCAAGTC CCTGCTGCAG ATCCAGATCA GTGACTGCAG CTCGGAGCAG 
ATTGAAGGTT TGTCGACTCT CAGGACGAGT TTGGAGACGA TGAACGTCCA CCAGAGTACA 
CAATCCATGA TGTCAGTTCT GGTCCCAGAG GCGGCGGAGT TCCCCCAGTG GGAACCTGAG 
GGGGTGGAGT CTGTGTGTCC TGTCACGGCA TTGGTGCCGG TGTGGTGTCA GCTGACCACC 
CTGGACCTGA GTCACAATGA CATCAGCTGC ATAGACAACT CTGTGAGGTT GATTCCAAAG 
GTGGAGTTCC TGGATCTGAG CTCCAATCAG CTGTCCTCAG TGGAGAACCT GCAGCACCTG 
TACAACCTGG TCCACGTGGA TCTGTCCTAC AACAACCTGC AGGTTCTGGA AGCTGCTCAC 
ACCCGTCTGG GGAACATTAA GACCCTGAAC CTGGCTGGAA ACCAGCTGCA GAACCTCAGT 
GGACTCTCCA AGCTCTACTC TCTGGTCAAC CTGGACCTCA GCCACAACCA GCTGGACCAG 
CTGGAGGAGA TCAGGAACAT TGGTTCTCTG CCCTGCCTGG AGAAGCTCAG CCTGGCCGGG 
AACCCCATGT GCATCATCCC CGACTACAGA ACCAAAGTCC TGGCCCAGTT TGGAGACCGA 
GCAGCTGAGG TGTGTCTGGA CAGCAGAGTG ACCACAGAGA AGGAGCTGGA CACCGTGGAG 
GTTCTCAAAG CCATCCAGAA GGCCAAAGAG GTCAAAGACC GGATGAGCAG CGGAGACAAG 
AAGATCAGTG AGGACACCAG ACCTCCTGCT GCTCCTGTCC TCTGCTCCTC CTCTTCCTCT 
CTGTCCTCCT CTGCTCCTCC TGCCTCCTCC TCCTCCTCCT GTCCGGCCCA GCAGGCTTCC 
AGCCAAGAAG TGACCAGCCC AGAAGAAGCC GCCGCCCCCC CCGCTGTTCT CCCCCCTGTA 
GACGCTACAA CCCCCCCTGC TAGCTTCAAC GCCCCCCACC TGTCTGCTGG CCCCGCCCAG 
GTCCAGCAGT CGTCCATCAG TCTGTCTGAA CTCTGTGGAG CTTCCACTGA TGTTCCTGCT 
GCTGATATCA CAGTCTGCTC TACCTGCTAC TCCTCCATCA CCTGTTCCTC CTGCAGCGCC 
CCCTGGTGTC CGCTGCTTCC TGCTCACCTG CTGCCGCTCT CCGCCTCCAA CCAGGACTTC 
GTTACCCAGC TGTCCCAGCG GCTCGGCTCC GCCCACAGGA AGCAGAGGCT GGAGGGGGAG 
GTGGAGGAGG AGAAGCCTGG ATCTGGAGAG CTTCTTCAGT CTGCTGGGGA TAGAACGGAG 
GTGGACGGTC TCAGCCCCGG CTCCAGGGAC GGCTACTTTG AGATGGGTCT GGGCGACTCT 
CTGGAGGAGG TGTTCTGCTC CTCCTCCATC GGCCTGCAGG TGGCGCCCAG CGAGGAGCAG 
CAGCAGCAGC AGCAGGAGGA AGAGGAGGAG GAAGAGGAGG AGGAGGAGGA GGTGAGGGTC 
AGCAGGGTTC TGTGGTGCTG CTGTGTTCTG GTGGAACATC AGGAGGAGCT GAAGCAGAGG 
GAGGCCTGTC TGGTTCTGAC CCACCACCTG CTGGGTCTGC TCTTCCTGTC ACCTGACTCC 
ACGGGGACCA ATCAGGACGC AGGTGAGCGG CTGGTGTTTG TTTGCAGGAT TTCTTTTTAT 
GTTCTCCTCA GCTGGAACTC CTCCCGTCCC CATGTGGAGG AGAACCATCC GTCCCCCCAC 
CTCCTGCCGG GTCTCTCCCC AGGACTGAAG CTCCTGGCCG GGCTGGGCGG GCAACAGCTG 
CTCGGCTACT TCCACAGATT CATCGCTCTG TCTGAGTCAG AGGATCTGAA GCAGGTTCTG 
TGGCTGTCGG TGGTTCTTTA TAAATCTCCA GAAGCTGAAC TCACCTGCTG CCTTCTGCTG 
TCCACCGACC ACATCTACTT CCTGCTGGAA GACTCCGCCT CCACGCTGAC GCATCACTCA 
GTCCTGGAGG TCTCTGATGC TGGAGATCCA GACTCCATCC TGGTCTGCTG TCTGTCCATC 
AGTCTGTCTG AGCTGCTGGC GGTGAACGTG GGTCTGTTCG ACCAGTACTT CAGAGTTGTA 
GGTAGATCAG CTGATCACAT CGTCTGCTGC CTCAGCAGGG ACAGTTACGG AACCGGAGTC 
TTCCTGCAGG AGCTGATGGC AGCACTGAGC CTCCAGCAGC AGCTTCCTCC TCCAGAGCCC 
TCCGACCAGG ACTTCTACTC CCAGTTCACC CACACCTGCT CAGGTAAGAT GCAGAACTAC 
GAGCTGGTCC ACAGCAGCCA GGTGAAGTTC ATCTACCCCA GCGAGGAGGA GATGGGAGAC 
CTGACCTTCA TCGTAGCAGA GAGGAAGACT CCGGCCAGCT GCTCCAGATC CTTCAATGTC 
CTGCTCTACC TGCTGGTGTT CCAGGTTCAG GTTCCGTCCA GTCAGAGTTC TGGTCCATCT 
GCTGTCCTCC AGCCCAGAAC TCTGATCCTC ACCAGCACCG ACGTCTTCCT GCTGGACGAG 
GACTACGTCA GCTACCCGCT GCCAGACTTC GCCAAAGAGC CTCCATCCAG GGAGCGCTAC 
CAGCTGCGTG AGGCCCGGCG GATCCGGGAC CTGGACCGAG TACTGCTGGG GTACCAGACC 
TACCCTCAGG CTCTGACGCT GGTGTTTGAC GACCTGCCCG GACCCGACCT GCTCTGCCAC 
CTCACCATGG ACCACTTTGC CGCCGGCGGG GAGGAGGCTC TGCCCAGAGG GGGCGGGGCC 
AGCAGGGGCG CAGAGGGTGA GGTCCAATGG TGCGTCTTCG TGCCTGGAGC CGACAGCAGA 
GAGAGGCTGA TCTCACTGCT GGCCCGCCAG TGGGAGGCGC AGTGCAGCCG CGAGCTGCCC 
GTGGAGCTCA CCGGCTGA