srgap2 cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis)

Gene
Clones
gRNAs

Summary

Gene Symbol	srgap2
Entrez Gene ID	110953346
Full Name	SLIT-ROBO Rho GTPase activating protein 2
Gene Type	protein-coding
Organism	Acanthochromis polyacanthus(spiny chromis)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022197301.1	XP_022052993.1	SLIT-ROBO Rho GTPase-activating protein 2 isoform X1
XM_022197303.1	XP_022052995.1	SLIT-ROBO Rho GTPase-activating protein 2 isoform X2

Pathways from BioSystems
Gene Ontology

The following srgap2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the srgap2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc194602	XM_022197301.1 Latest version!	Acanthochromis polyacanthus SLIT-ROBO Rho GTPase activating protein 2 (srgap2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAc194603	XM_022197303.1 Latest version!	Acanthochromis polyacanthus SLIT-ROBO Rho GTPase activating protein 2 (srgap2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

srgap2 ( XM_022197301.1 ) cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis) -> XP_022052993.1 Acanthochromis polyacanthus SLIT-ROBO Rho GTPase activating protein 2 (srgap2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAc194602
Clone ID Related Accession (Same CDS sequence)	XM_022197301.1
Accession Version	XM_022197301.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-06
Organism	Acanthochromis polyacanthus(spiny chromis)
Product	SLIT-ROBO Rho GTPase-activating protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019029406.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthochromis polyacanthus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTC TTAATGAGCT TTACACGGTG ATGAAGACGT ACCACATGTA CAACACCGAC 
AGCATCAATG CCGAGTCCAA GCTGAAGGAG GCAGAGAAGC AGGAGGAGAA GCAGATGGGA 
CGCTCGAGTC GACAGGATGA CCGTCAGACG CCGCGCTCCC CCGACACCTT GGCCAGCATT 
AAGACGGACG AGAAACCCGT CCGACGCTCC AGTGTCAAAA AAATCGAGAA GATGAAGGAG 
AAGAGACAAG CTAAATACAC AGAGAACAAG CTGAAGGCCA TCAAGGCCAG GAATGAATAC 
CTGCTAGCTC TTGAGGCTAC CAACGGCTGT GTCTTCAAAT ATTACATCCA TGACCTCTCT 
GACATCATTG ATTGCTGTGA CCTGGGCTAC CATGCCAGCC TGCATCGTGC TCTGAGGACC 
TACTTATCTG CAGAACAGAA TGTAGAGACG TCCAAACACA CCGGCCTAGA GACACTGGAA 
GGAGCGGCCG AGAGTTTGGA GGCCAACGGT GACAAACAGA AACTGATGGA GACCTACAAC 
AACGTCTTCT GCCCTCCGGC TCGCTTTGAC TTCCAGTCCC ACATGGGAGA CACGATGGGT 
GTGATGTGTG CACAGCAGCC GGTGGAAGGC GAGCTTCTCC AGAGGTGTCA GCAGCTGCAG 
TCCCGCCTCT CCACACTGAA GATTGAGAAT GAAGAGGTGA AGAAGACCAT GGAGGCCACT 
CTGTCCACCA TTCAAGACAT GGTGACGGTG GAGGACTATG ACGTGTCTGA GTGCTTCCAC 
CACAGCAACA GCATGGAGTC AGTCAAGTCC ACTTTCAGTG AATCCTATCT CAGCAAGCCC 
AGCCTGGCCA AAAGACGGGC CAATCAGCAG GAGACGGAGC AATTTTACTT CACAAAGCTG 
AAGGAGTTTC TGGAAGGCAG GAACCTGATC ACCAAGCTGG AGGCTAAGCA CGACCTCATC 
GAGAAGACCC TTGGAGAGAA TCAGAAGACT GACTGTTGCC TTGCCAGTGG ACGGAGAAAC 
TCCACAGTAC GGAAGCAGGA GTCGGGTGAA GCCATTCCTC TGATGGTCGA GAGCTGCATC 
CGCTTCATCA GTCGCCATGG TCTGCAGCAT GAGGGAATCT TCAGAGTGTC AGGCTCTCAG 
GTGGAAGTCA ATGACATCAA GAACGCTTTT GAGAGAGGTG AGGACCCACT GGCTGGGGAC 
CAGAATGATC ATGACATGGA CTCTATTGCT GGTGTACTGA AGCTGTACTT TAGAGGGCTG 
GACCACGCCC TCTTCCCTAA AGAAGTCTTC CATGACCTCA TATCCTGTGT CTCAATGGAG 
AGCCTCCAGG AGCGGGCAGT CCACATTAAG AAAGTTCTGC AGTCCCTGCC AAGCAAAACC 
CTTATCATCA TGAGATACTT GTTTGCCTTC CTCAACCACC TGTCCCAGTA CAGCGAGGAC 
AACATGATGG ACCCCTACAA CCTGGCCATC TGTTTCGGCC CCACCCTGAT GTCTGTCCCA 
GAGGGCCACG ACCAGGTCTC TTGCCAGGCC CACGTCAACG AGCTCATTAA AACTATCATC 
ATCCACCATG ACACCATCTT TCCTGGGCCA CAGGACCTGC AGGGCCCCAT CTACACCATC 
CCCGGAGCAG GAGACGACTT CTGTGACAGT CCACACTGTG AGCCCCCCCT TGTGGAGGAG 
CCGGCCCCTG ACACCGTCTC TGTCACCCAC AACAGTGAAG ATGGCTCCTT GGCTGTTTCA 
GAGTCCGACC CGATCGAAGC CATCGCTCGG TTCGACTACA CCGGACGAAC CGCTCGGGAG 
CTATCATTCA AGAAAGGTGC CTCTCTGCTG CTGTACCAGC GAGCCTCTGA TGACTGGTGG 
GAGGGTCGGC ACAATGGAGT GGATGGACTG GTGCCGCACC AGTACATTGT GGTCCAAGAC 
ACGTCTGATG TGGGACGGGG AAGTCCAAAG CCTGATGTGG ACAGCAGGGA CCTGCTGGAG 
GAGAGAGTGT CCACTAGAGG CAGCGCTGCC TCTCCTACAG GAGCTCATGT GGCTGACATC 
TATCTGGCCA ATCTGAACAA GTTGAGGAAA CGGCCAGAGC CCGGGAACAT CCGAAGACCG 
TTCCGCTCAG AGAGCGACAC TACGAGTCCA GGAGCCAGTG GAGGGGGAGG AGGAGTGAGA 
ACAGCTTCTC TTCCTGTTGG CAGTGCTCTG GTAAAAGAAG CAGGAGATAA ACGACCAGTC 
AGTGCTCACA GCATCCTCAA CTCTGTCACT CGCCACTCCT CACTCAAGAC CAAGGTGGAG 
AGTCCACAGT TGCGGAAGAC AACTTCAGCA GGACGGTCCA AGAGCTTCAG CAACCACAGA 
CCGCTGGACC CTGAGGTCAT AGGCCAGGTG GAGCACAGCT CACAGGACAT TGAGGCGACC 
ATGAGCTCTA CCCTGAGTGA GCTCAGTAAG CTGGAAAGAC AAGGCACCCC GAAGCATACA 
CACACCCCTG ACGTGGTCCT GGACACACTG GAGCAGCTGA AAGGTGTTGG AGGCGGTGGA 
GGAGCTTCAG AGCCTTCTAG TCCACTTCAC TCCCGTCTGC TGCGGGACAG CGAGGGGGGC 
TCCTCACACG CCCATCCACT TCAGCGCAGC GCCTCCTCAG CCAGCGACGT TCCCTCCTCC 
TTCCGCCCTG CCAAGAGCCA ACCGCGGAGC CCCCTGCCCT CGGCCACGTC CCCCTCTCTG 
TCCTCCTCCT CTCTCTCCTC ATCTGTACCT TCCTTTAGAG AGCTGCGCCC TCCAGCTACA 
AGGCCCAAGC CGGTGGTGTT CCCAAAGAGC GGCGGAGGGA GCGGCAGTCC AGCGATGGGC 
TCCCCGACCT CCACCGTTCC CCCTACGCCT CCTCCTCCCC CAGCAGGCCA CACACACTCG 
CTCCCTCACA CCCCTCCTCC CCCTCCGCCA CCCCAGTCTA ATGACAAATC CTGCCCGGCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022052993.1
CDS	224..3166
Translation	MKVLNELYTVMKTYHMYNTDSINAESKLKEAEKQEEKQMGRSSRQDDRQTPRSPDTLASIKTDEKPVRRSSVKKIEKMKEKRQAKYTENKLKAIKARNEYLLALEATNGCVFKYYIHDLSDIIDCCDLGYHASLHRALRTYLSAEQNVETSKHTGLETLEGAAESLEANGDKQKLMETYNNVFCPPARFDFQSHMGDTMGVMCAQQPVEGELLQRCQQLQSRLSTLKIENEEVKKTMEATLSTIQDMVTVEDYDVSECFHHSNSMESVKSTFSESYLSKPSLAKRRANQQETEQFYFTKLKEFLEGRNLITKLEAKHDLIEKTLGENQKTDCCLASGRRNSTVRKQESGEAIPLMVESCIRFISRHGLQHEGIFRVSGSQVEVNDIKNAFERGEDPLAGDQNDHDMDSIAGVLKLYFRGLDHALFPKEVFHDLISCVSMESLQERAVHIKKVLQSLPSKTLIIMRYLFAFLNHLSQYSEDNMMDPYNLAICFGPTLMSVPEGHDQVSCQAHVNELIKTIIIHHDTIFPGPQDLQGPIYTIPGAGDDFCDSPHCEPPLVEEPAPDTVSVTHNSEDGSLAVSESDPIEAIARFDYTGRTARELSFKKGASLLLYQRASDDWWEGRHNGVDGLVPHQYIVVQDTSDVGRGSPKPDVDSRDLLEERVSTRGSAASPTGAHVADIYLANLNKLRKRPEPGNIRRPFRSESDTTSPGASGGGGGVRTASLPVGSALVKEAGDKRPVSAHSILNSVTRHSSLKTKVESPQLRKTTSAGRSKSFSNHRPLDPEVIGQVEHSSQDIEATMSSTLSELSKLERQGTPKHTHTPDVVLDTLEQLKGVGGGGGASEPSSPLHSRLLRDSEGGSSHAHPLQRSASSASDVPSSFRPAKSQPRSPLPSATSPSLSSSSLSSSVPSFRELRPPATRPKPVVFPKSGGGSGSPAMGSPTSTVPPTPPPPPAGHTHSLPHTPPPPPPPQSNDKSCPA

Target ORF information:

RefSeq Version	XM_022197301.1
Organism	Acanthochromis polyacanthus(spiny chromis)
Definition	Acanthochromis polyacanthus SLIT-ROBO Rho GTPase activating protein 2 (srgap2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022197301.1

ORF Insert Sequence:

ATGAAAGTTC TTAATGAGCT TTACACGGTG ATGAAGACGT ACCACATGTA CAACACCGAC 
AGCATCAATG CCGAGTCCAA GCTGAAGGAG GCAGAGAAGC AGGAGGAGAA GCAGATGGGA 
CGCTCGAGTC GACAGGATGA CCGTCAGACG CCGCGCTCCC CCGACACCTT GGCCAGCATT 
AAGACGGACG AGAAACCCGT CCGACGCTCC AGTGTCAAAA AAATCGAGAA GATGAAGGAG 
AAGAGACAAG CTAAATACAC AGAGAACAAG CTGAAGGCCA TCAAGGCCAG GAATGAATAC 
CTGCTAGCTC TTGAGGCTAC CAACGGCTGT GTCTTCAAAT ATTACATCCA TGACCTCTCT 
GACATCATTG ATTGCTGTGA CCTGGGCTAC CATGCCAGCC TGCATCGTGC TCTGAGGACC 
TACTTATCTG CAGAACAGAA TGTAGAGACG TCCAAACACA CCGGCCTAGA GACACTGGAA 
GGAGCGGCCG AGAGTTTGGA GGCCAACGGT GACAAACAGA AACTGATGGA GACCTACAAC 
AACGTCTTCT GCCCTCCGGC TCGCTTTGAC TTCCAGTCCC ACATGGGAGA CACGATGGGT 
GTGATGTGTG CACAGCAGCC GGTGGAAGGC GAGCTTCTCC AGAGGTGTCA GCAGCTGCAG 
TCCCGCCTCT CCACACTGAA GATTGAGAAT GAAGAGGTGA AGAAGACCAT GGAGGCCACT 
CTGTCCACCA TTCAAGACAT GGTGACGGTG GAGGACTATG ACGTGTCTGA GTGCTTCCAC 
CACAGCAACA GCATGGAGTC AGTCAAGTCC ACTTTCAGTG AATCCTATCT CAGCAAGCCC 
AGCCTGGCCA AAAGACGGGC CAATCAGCAG GAGACGGAGC AATTTTACTT CACAAAGCTG 
AAGGAGTTTC TGGAAGGCAG GAACCTGATC ACCAAGCTGG AGGCTAAGCA CGACCTCATC 
GAGAAGACCC TTGGAGAGAA TCAGAAGACT GACTGTTGCC TTGCCAGTGG ACGGAGAAAC 
TCCACAGTAC GGAAGCAGGA GTCGGGTGAA GCCATTCCTC TGATGGTCGA GAGCTGCATC 
CGCTTCATCA GTCGCCATGG TCTGCAGCAT GAGGGAATCT TCAGAGTGTC AGGCTCTCAG 
GTGGAAGTCA ATGACATCAA GAACGCTTTT GAGAGAGGTG AGGACCCACT GGCTGGGGAC 
CAGAATGATC ATGACATGGA CTCTATTGCT GGTGTACTGA AGCTGTACTT TAGAGGGCTG 
GACCACGCCC TCTTCCCTAA AGAAGTCTTC CATGACCTCA TATCCTGTGT CTCAATGGAG 
AGCCTCCAGG AGCGGGCAGT CCACATTAAG AAAGTTCTGC AGTCCCTGCC AAGCAAAACC 
CTTATCATCA TGAGATACTT GTTTGCCTTC CTCAACCACC TGTCCCAGTA CAGCGAGGAC 
AACATGATGG ACCCCTACAA CCTGGCCATC TGTTTCGGCC CCACCCTGAT GTCTGTCCCA 
GAGGGCCACG ACCAGGTCTC TTGCCAGGCC CACGTCAACG AGCTCATTAA AACTATCATC 
ATCCACCATG ACACCATCTT TCCTGGGCCA CAGGACCTGC AGGGCCCCAT CTACACCATC 
CCCGGAGCAG GAGACGACTT CTGTGACAGT CCACACTGTG AGCCCCCCCT TGTGGAGGAG 
CCGGCCCCTG ACACCGTCTC TGTCACCCAC AACAGTGAAG ATGGCTCCTT GGCTGTTTCA 
GAGTCCGACC CGATCGAAGC CATCGCTCGG TTCGACTACA CCGGACGAAC CGCTCGGGAG 
CTATCATTCA AGAAAGGTGC CTCTCTGCTG CTGTACCAGC GAGCCTCTGA TGACTGGTGG 
GAGGGTCGGC ACAATGGAGT GGATGGACTG GTGCCGCACC AGTACATTGT GGTCCAAGAC 
ACGTCTGATG TGGGACGGGG AAGTCCAAAG CCTGATGTGG ACAGCAGGGA CCTGCTGGAG 
GAGAGAGTGT CCACTAGAGG CAGCGCTGCC TCTCCTACAG GAGCTCATGT GGCTGACATC 
TATCTGGCCA ATCTGAACAA GTTGAGGAAA CGGCCAGAGC CCGGGAACAT CCGAAGACCG 
TTCCGCTCAG AGAGCGACAC TACGAGTCCA GGAGCCAGTG GAGGGGGAGG AGGAGTGAGA 
ACAGCTTCTC TTCCTGTTGG CAGTGCTCTG GTAAAAGAAG CAGGAGATAA ACGACCAGTC 
AGTGCTCACA GCATCCTCAA CTCTGTCACT CGCCACTCCT CACTCAAGAC CAAGGTGGAG 
AGTCCACAGT TGCGGAAGAC AACTTCAGCA GGACGGTCCA AGAGCTTCAG CAACCACAGA 
CCGCTGGACC CTGAGGTCAT AGGCCAGGTG GAGCACAGCT CACAGGACAT TGAGGCGACC 
ATGAGCTCTA CCCTGAGTGA GCTCAGTAAG CTGGAAAGAC AAGGCACCCC GAAGCATACA 
CACACCCCTG ACGTGGTCCT GGACACACTG GAGCAGCTGA AAGGTGTTGG AGGCGGTGGA 
GGAGCTTCAG AGCCTTCTAG TCCACTTCAC TCCCGTCTGC TGCGGGACAG CGAGGGGGGC 
TCCTCACACG CCCATCCACT TCAGCGCAGC GCCTCCTCAG CCAGCGACGT TCCCTCCTCC 
TTCCGCCCTG CCAAGAGCCA ACCGCGGAGC CCCCTGCCCT CGGCCACGTC CCCCTCTCTG 
TCCTCCTCCT CTCTCTCCTC ATCTGTACCT TCCTTTAGAG AGCTGCGCCC TCCAGCTACA 
AGGCCCAAGC CGGTGGTGTT CCCAAAGAGC GGCGGAGGGA GCGGCAGTCC AGCGATGGGC 
TCCCCGACCT CCACCGTTCC CCCTACGCCT CCTCCTCCCC CAGCAGGCCA CACACACTCG 
CTCCCTCACA CCCCTCCTCC CCCTCCGCCA CCCCAGTCTA ATGACAAATC CTGCCCGGCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

srgap2 ( XM_022197303.1 ) cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis) -> XP_022052995.1 Acanthochromis polyacanthus SLIT-ROBO Rho GTPase activating protein 2 (srgap2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAc194603
Clone ID Related Accession (Same CDS sequence)	XM_022197303.1
Accession Version	XM_022197303.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-06
Organism	Acanthochromis polyacanthus(spiny chromis)
Product	SLIT-ROBO Rho GTPase-activating protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019029406.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthochromis polyacanthus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTC TTAATGAGCT TTACACGGTG ATGAAGACGT ACCACATGTA CAACACCGAC 
AGCATCAATG CCGAGTCCAA GCTGAAGGAG GCAGAGAAGC AGGAGGAGAA GCAGATGGGA 
CGCTCGAGTC GACAGGATGA CCGTCAGACG CCGCGCTCCC CCGACACCTT GGCCAGCATT 
AAGACGGACG AGAAACCCGT CCGACGCTCC AGTGTCAAAA AAATCGAGAA GATGAAGGAG 
AAGAGACAAG CTAAATACAC AGAGAACAAG CTGAAGGCCA TCAAGGCCAG GAATGAATAC 
CTGCTAGCTC TTGAGGCTAC CAACGGCTGT GTCTTCAAAT ATTACATCCA TGACCTCTCT 
GACATCATTG ATTGCTGTGA CCTGGGCTAC CATGCCAGCC TGCATCGTGC TCTGAGGACC 
TACTTATCTG CAGAACAGAA TGTAGAGACG TCCAAACACA CCGGCCTAGA GACACTGGAA 
GGAGCGGCCG AGAGTTTGGA GGCCAACGGT GACAAACAGA AACTGATGGA GACCTACAAC 
AACGTCTTCT GCCCTCCGGC TCGCTTTGAC TTCCAGTCCC ACATGGGAGA CACGATGGGT 
GTGATGTGTG CACAGCAGCC GGTGGAAGGC GAGCTTCTCC AGAGGTGTCA GCAGCTGCAG 
TCCCGCCTCT CCACACTGAA GATTGAGAAT GAAGAGGTGA AGAAGACCAT GGAGGCCACT 
CTGTCCACCA TTCAAGACAT GGTGACGGTG GAGGACTATG ACGTGTCTGA GTGCTTCCAC 
CACAGCAACA GCATGGAGTC AGTCAAGTCC ACTTTCAGTG AATCCTATCT CAGCAAGCCC 
AGCCTGGCCA AAAGACGGGC CAATCAGCAG GAGACGGAGC AATTTTACTT CACAAAGCTG 
AAGGAGTTTC TGGAAGGCAG GAACCTGATC ACCAAGCTGG AGGCTAAGCA CGACCTCATC 
GAGAAGACCC TTGGAGAGAA TCAGAAGACT GACTGTTGCC TTGCCAGTGG ACGGAGAAAC 
TCCACAGTAC GGAAGCAGGA GTCGGGTGAA GCCATTCCTC TGATGGTCGA GAGCTGCATC 
CGCTTCATCA GTCGCCATGG TCTGCAGCAT GAGGGAATCT TCAGAGTGTC AGGCTCTCAG 
GTGGAAGTCA ATGACATCAA GAACGCTTTT GAGAGAGGTG AGGACCCACT GGCTGGGGAC 
CAGAATGATC ATGACATGGA CTCTATTGCT GGTGTACTGA AGCTGTACTT TAGAGGGCTG 
GACCACGCCC TCTTCCCTAA AGAAGTCTTC CATGACCTCA TATCCTGTGT CTCAATGGAG 
AGCCTCCAGG AGCGGGCAGT CCACATTAAG AAAGTTCTGC AGTCCCTGCC AAGCAAAACC 
CTTATCATCA TGAGATACTT GTTTGCCTTC CTCAACCACC TGTCCCAGTA CAGCGAGGAC 
AACATGATGG ACCCCTACAA CCTGGCCATC TGTTTCGGCC CCACCCTGAT GTCTGTCCCA 
GAGGGCCACG ACCAGGTCTC TTGCCAGGCC CACGTCAACG AGCTCATTAA AACTATCATC 
ATCCACCATG ACACCATCTT TCCTGGGCCA CAGGACCTGC AGGGCCCCAT CTACACCATC 
CCCGGAGCAG GAGACGACTT CTGTGACAGT CCACACTGTG AGCCCCCCCT TGTGGAGGAG 
CCGGCCCCTG ACACCGTCTC TGTCACCCAC AACAGTGAAG ATGAGTCCGA CCCGATCGAA 
GCCATCGCTC GGTTCGACTA CACCGGACGA ACCGCTCGGG AGCTATCATT CAAGAAAGGT 
GCCTCTCTGC TGCTGTACCA GCGAGCCTCT GATGACTGGT GGGAGGGTCG GCACAATGGA 
GTGGATGGAC TGGTGCCGCA CCAGTACATT GTGGTCCAAG ACACGTCTGA TGTGGGACGG 
GGAAGTCCAA AGCCTGATGT GGACAGCAGG GACCTGCTGG AGGAGAGAGT GTCCACTAGA 
GGCAGCGCTG CCTCTCCTAC AGGAGCTCAT GTGGCTGACA TCTATCTGGC CAATCTGAAC 
AAGTTGAGGA AACGGCCAGA GCCCGGGAAC ATCCGAAGAC CGTTCCGCTC AGAGAGCGAC 
ACTACGAGTC CAGGAGCCAG TGGAGGGGGA GGAGGAGTGA GAACAGCTTC TCTTCCTGTT 
GGCAGTGCTC TGGTAAAAGA AGCAGGAGAT AAACGACCAG TCAGTGCTCA CAGCATCCTC 
AACTCTGTCA CTCGCCACTC CTCACTCAAG ACCAAGGTGG AGAGTCCACA GTTGCGGAAG 
ACAACTTCAG CAGGACGGTC CAAGAGCTTC AGCAACCACA GACCGCTGGA CCCTGAGGTC 
ATAGGCCAGG TGGAGCACAG CTCACAGGAC ATTGAGGCGA CCATGAGCTC TACCCTGAGT 
GAGCTCAGTA AGCTGGAAAG ACAAGGCACC CCGAAGCATA CACACACCCC TGACGTGGTC 
CTGGACACAC TGGAGCAGCT GAAAGGTGTT GGAGGCGGTG GAGGAGCTTC AGAGCCTTCT 
AGTCCACTTC ACTCCCGTCT GCTGCGGGAC AGCGAGGGGG GCTCCTCACA CGCCCATCCA 
CTTCAGCGCA GCGCCTCCTC AGCCAGCGAC GTTCCCTCCT CCTTCCGCCC TGCCAAGAGC 
CAACCGCGGA GCCCCCTGCC CTCGGCCACG TCCCCCTCTC TGTCCTCCTC CTCTCTCTCC 
TCATCTGTAC CTTCCTTTAG AGAGCTGCGC CCTCCAGCTA CAAGGCCCAA GCCGGTGGTG 
TTCCCAAAGA GCGGCGGAGG GAGCGGCAGT CCAGCGATGG GCTCCCCGAC CTCCACCGTT 
CCCCCTACGC CTCCTCCTCC CCCAGCAGGC CACACACACT CGCTCCCTCA CACCCCTCCT 
CCCCCTCCGC CACCCCAGTC TAATGACAAA TCCTGCCCGG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022052995.1
CDS	224..3148
Translation	MKVLNELYTVMKTYHMYNTDSINAESKLKEAEKQEEKQMGRSSRQDDRQTPRSPDTLASIKTDEKPVRRSSVKKIEKMKEKRQAKYTENKLKAIKARNEYLLALEATNGCVFKYYIHDLSDIIDCCDLGYHASLHRALRTYLSAEQNVETSKHTGLETLEGAAESLEANGDKQKLMETYNNVFCPPARFDFQSHMGDTMGVMCAQQPVEGELLQRCQQLQSRLSTLKIENEEVKKTMEATLSTIQDMVTVEDYDVSECFHHSNSMESVKSTFSESYLSKPSLAKRRANQQETEQFYFTKLKEFLEGRNLITKLEAKHDLIEKTLGENQKTDCCLASGRRNSTVRKQESGEAIPLMVESCIRFISRHGLQHEGIFRVSGSQVEVNDIKNAFERGEDPLAGDQNDHDMDSIAGVLKLYFRGLDHALFPKEVFHDLISCVSMESLQERAVHIKKVLQSLPSKTLIIMRYLFAFLNHLSQYSEDNMMDPYNLAICFGPTLMSVPEGHDQVSCQAHVNELIKTIIIHHDTIFPGPQDLQGPIYTIPGAGDDFCDSPHCEPPLVEEPAPDTVSVTHNSEDESDPIEAIARFDYTGRTARELSFKKGASLLLYQRASDDWWEGRHNGVDGLVPHQYIVVQDTSDVGRGSPKPDVDSRDLLEERVSTRGSAASPTGAHVADIYLANLNKLRKRPEPGNIRRPFRSESDTTSPGASGGGGGVRTASLPVGSALVKEAGDKRPVSAHSILNSVTRHSSLKTKVESPQLRKTTSAGRSKSFSNHRPLDPEVIGQVEHSSQDIEATMSSTLSELSKLERQGTPKHTHTPDVVLDTLEQLKGVGGGGGASEPSSPLHSRLLRDSEGGSSHAHPLQRSASSASDVPSSFRPAKSQPRSPLPSATSPSLSSSSLSSSVPSFRELRPPATRPKPVVFPKSGGGSGSPAMGSPTSTVPPTPPPPPAGHTHSLPHTPPPPPPPQSNDKSCPA

Target ORF information:

RefSeq Version	XM_022197303.1
Organism	Acanthochromis polyacanthus(spiny chromis)
Definition	Acanthochromis polyacanthus SLIT-ROBO Rho GTPase activating protein 2 (srgap2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022197303.1

ORF Insert Sequence:

ATGAAAGTTC TTAATGAGCT TTACACGGTG ATGAAGACGT ACCACATGTA CAACACCGAC 
AGCATCAATG CCGAGTCCAA GCTGAAGGAG GCAGAGAAGC AGGAGGAGAA GCAGATGGGA 
CGCTCGAGTC GACAGGATGA CCGTCAGACG CCGCGCTCCC CCGACACCTT GGCCAGCATT 
AAGACGGACG AGAAACCCGT CCGACGCTCC AGTGTCAAAA AAATCGAGAA GATGAAGGAG 
AAGAGACAAG CTAAATACAC AGAGAACAAG CTGAAGGCCA TCAAGGCCAG GAATGAATAC 
CTGCTAGCTC TTGAGGCTAC CAACGGCTGT GTCTTCAAAT ATTACATCCA TGACCTCTCT 
GACATCATTG ATTGCTGTGA CCTGGGCTAC CATGCCAGCC TGCATCGTGC TCTGAGGACC 
TACTTATCTG CAGAACAGAA TGTAGAGACG TCCAAACACA CCGGCCTAGA GACACTGGAA 
GGAGCGGCCG AGAGTTTGGA GGCCAACGGT GACAAACAGA AACTGATGGA GACCTACAAC 
AACGTCTTCT GCCCTCCGGC TCGCTTTGAC TTCCAGTCCC ACATGGGAGA CACGATGGGT 
GTGATGTGTG CACAGCAGCC GGTGGAAGGC GAGCTTCTCC AGAGGTGTCA GCAGCTGCAG 
TCCCGCCTCT CCACACTGAA GATTGAGAAT GAAGAGGTGA AGAAGACCAT GGAGGCCACT 
CTGTCCACCA TTCAAGACAT GGTGACGGTG GAGGACTATG ACGTGTCTGA GTGCTTCCAC 
CACAGCAACA GCATGGAGTC AGTCAAGTCC ACTTTCAGTG AATCCTATCT CAGCAAGCCC 
AGCCTGGCCA AAAGACGGGC CAATCAGCAG GAGACGGAGC AATTTTACTT CACAAAGCTG 
AAGGAGTTTC TGGAAGGCAG GAACCTGATC ACCAAGCTGG AGGCTAAGCA CGACCTCATC 
GAGAAGACCC TTGGAGAGAA TCAGAAGACT GACTGTTGCC TTGCCAGTGG ACGGAGAAAC 
TCCACAGTAC GGAAGCAGGA GTCGGGTGAA GCCATTCCTC TGATGGTCGA GAGCTGCATC 
CGCTTCATCA GTCGCCATGG TCTGCAGCAT GAGGGAATCT TCAGAGTGTC AGGCTCTCAG 
GTGGAAGTCA ATGACATCAA GAACGCTTTT GAGAGAGGTG AGGACCCACT GGCTGGGGAC 
CAGAATGATC ATGACATGGA CTCTATTGCT GGTGTACTGA AGCTGTACTT TAGAGGGCTG 
GACCACGCCC TCTTCCCTAA AGAAGTCTTC CATGACCTCA TATCCTGTGT CTCAATGGAG 
AGCCTCCAGG AGCGGGCAGT CCACATTAAG AAAGTTCTGC AGTCCCTGCC AAGCAAAACC 
CTTATCATCA TGAGATACTT GTTTGCCTTC CTCAACCACC TGTCCCAGTA CAGCGAGGAC 
AACATGATGG ACCCCTACAA CCTGGCCATC TGTTTCGGCC CCACCCTGAT GTCTGTCCCA 
GAGGGCCACG ACCAGGTCTC TTGCCAGGCC CACGTCAACG AGCTCATTAA AACTATCATC 
ATCCACCATG ACACCATCTT TCCTGGGCCA CAGGACCTGC AGGGCCCCAT CTACACCATC 
CCCGGAGCAG GAGACGACTT CTGTGACAGT CCACACTGTG AGCCCCCCCT TGTGGAGGAG 
CCGGCCCCTG ACACCGTCTC TGTCACCCAC AACAGTGAAG ATGAGTCCGA CCCGATCGAA 
GCCATCGCTC GGTTCGACTA CACCGGACGA ACCGCTCGGG AGCTATCATT CAAGAAAGGT 
GCCTCTCTGC TGCTGTACCA GCGAGCCTCT GATGACTGGT GGGAGGGTCG GCACAATGGA 
GTGGATGGAC TGGTGCCGCA CCAGTACATT GTGGTCCAAG ACACGTCTGA TGTGGGACGG 
GGAAGTCCAA AGCCTGATGT GGACAGCAGG GACCTGCTGG AGGAGAGAGT GTCCACTAGA 
GGCAGCGCTG CCTCTCCTAC AGGAGCTCAT GTGGCTGACA TCTATCTGGC CAATCTGAAC 
AAGTTGAGGA AACGGCCAGA GCCCGGGAAC ATCCGAAGAC CGTTCCGCTC AGAGAGCGAC 
ACTACGAGTC CAGGAGCCAG TGGAGGGGGA GGAGGAGTGA GAACAGCTTC TCTTCCTGTT 
GGCAGTGCTC TGGTAAAAGA AGCAGGAGAT AAACGACCAG TCAGTGCTCA CAGCATCCTC 
AACTCTGTCA CTCGCCACTC CTCACTCAAG ACCAAGGTGG AGAGTCCACA GTTGCGGAAG 
ACAACTTCAG CAGGACGGTC CAAGAGCTTC AGCAACCACA GACCGCTGGA CCCTGAGGTC 
ATAGGCCAGG TGGAGCACAG CTCACAGGAC ATTGAGGCGA CCATGAGCTC TACCCTGAGT 
GAGCTCAGTA AGCTGGAAAG ACAAGGCACC CCGAAGCATA CACACACCCC TGACGTGGTC 
CTGGACACAC TGGAGCAGCT GAAAGGTGTT GGAGGCGGTG GAGGAGCTTC AGAGCCTTCT 
AGTCCACTTC ACTCCCGTCT GCTGCGGGAC AGCGAGGGGG GCTCCTCACA CGCCCATCCA 
CTTCAGCGCA GCGCCTCCTC AGCCAGCGAC GTTCCCTCCT CCTTCCGCCC TGCCAAGAGC 
CAACCGCGGA GCCCCCTGCC CTCGGCCACG TCCCCCTCTC TGTCCTCCTC CTCTCTCTCC 
TCATCTGTAC CTTCCTTTAG AGAGCTGCGC CCTCCAGCTA CAAGGCCCAA GCCGGTGGTG 
TTCCCAAAGA GCGGCGGAGG GAGCGGCAGT CCAGCGATGG GCTCCCCGAC CTCCACCGTT 
CCCCCTACGC CTCCTCCTCC CCCAGCAGGC CACACACACT CGCTCCCTCA CACCCCTCCT 
CCCCCTCCGC CACCCCAGTC TAATGACAAA TCCTGCCCGG CTTAG