PEAK1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015468519.1	XP_015324005.1	pseudopodium-enriched atypical kinase 1
XM_015459261.1	XP_015314747.1	pseudopodium-enriched atypical kinase 1
XM_010817120.2	XP_010815422.1	pseudopodium-enriched atypical kinase 1
XM_015459262.1	XP_015314748.1	pseudopodium-enriched atypical kinase 1
XM_005222010.3	XP_005222067.1	pseudopodium-enriched atypical kinase 1
XM_005222004.4	XP_005222061.2	pseudopodium-enriched atypical kinase 1
XM_003587568.5	XP_003587616.2	pseudopodium-enriched atypical kinase 1
XM_003587568.4	XP_003587616.1	pseudopodium-enriched atypical kinase 1
XM_015468514.1	XP_015324000.1	pseudopodium-enriched atypical kinase 1
XM_015468516.1	XP_015324002.1	pseudopodium-enriched atypical kinase 1
XM_015468518.1	XP_015324004.1	pseudopodium-enriched atypical kinase 1
XM_005222010.4	XP_005222067.2	pseudopodium-enriched atypical kinase 1
XM_010798541.2	XP_010796843.1	pseudopodium-enriched atypical kinase 1
XM_015468517.1	XP_015324003.1	pseudopodium-enriched atypical kinase 1
XM_024982035.1	XP_024837803.1	pseudopodium-enriched atypical kinase 1
XM_024982037.1	XP_024837805.1	pseudopodium-enriched atypical kinase 1
XM_015468520.1	XP_015324006.1	pseudopodium-enriched atypical kinase 1
XM_005222009.3	XP_005222066.1	pseudopodium-enriched atypical kinase 1
XM_005222005.3	XP_005222062.1	pseudopodium-enriched atypical kinase 1
XM_005222005.4	XP_005222062.2	pseudopodium-enriched atypical kinase 1
XM_015459262.2	XP_015314748.2	pseudopodium-enriched atypical kinase 1
XM_010817120.3	XP_010815422.2	pseudopodium-enriched atypical kinase 1
XM_005222004.3	XP_005222061.1	pseudopodium-enriched atypical kinase 1
XM_015468515.1	XP_015324001.1	pseudopodium-enriched atypical kinase 1
XM_015459261.2	XP_015314747.2	pseudopodium-enriched atypical kinase 1
XM_024982036.1	XP_024837804.1	pseudopodium-enriched atypical kinase 1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa228456	XM_015459261.2 Latest version!	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228456	XM_024982035.1 Latest version!	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228456	XM_024982037.1 Latest version!	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228456	XM_005222010.4 Latest version!	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228456	XM_003587568.5 Latest version!	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228456	XM_010817120.3 Latest version!	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228456	XM_005222004.4 Latest version!	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228456	XM_024982036.1 Latest version!	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228456	XM_015459262.2 Latest version!	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228456	XM_005222005.4 Latest version!	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa10769	XM_015468514.1 Latest version!	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa10769	XM_005222005.3 Latest version!	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa10769	XM_015468515.1 Latest version!	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa10769	XM_005222004.3 Latest version!	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa10769	XM_010798541.2 Latest version!	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa10769	XM_003587568.4 Latest version!	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa10769	XM_010817120.2 Latest version!	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa10769	XM_015468519.1 Latest version!	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa10769	XM_015459262.1 Latest version!	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa10769	XM_015468518.1 Latest version!	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa10769	XM_015459261.1 Latest version!	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa10769	XM_005222009.3 Latest version!	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa10769	XM_015468517.1 Latest version!	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa10769	XM_015468516.1 Latest version!	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa10769	XM_015468520.1 Latest version!	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa10769	XM_005222010.3 Latest version!	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

PEAK1 ( XM_005222004.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222061.2 Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa228456
Clone ID Related Accession (Same CDS sequence)	XM_005222004.4 , XM_003587568.5 , XM_005222010.4 , XM_024982035.1 , XM_024982037.1 , XM_005222005.4 , XM_015459262.2 , XM_010817120.3 , XM_015459261.2 , XM_024982036.1
Accession Version	XM_005222004.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222004.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCAGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATC 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222061.2
CDS	520..5763
Translation	MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMMVADGQSVCGELSIQEHCENKPVVIGWNRNRMALNQKPLNNNNEDDVEGFSHVPKPYGNNDSAKKISNNNNGLTEVLKEIAGLDTTPQIKENETNSRETFLGRINDCYKRSLERKLPPSCMMGGMKDTQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLLSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTVPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGPGSTQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGIAPGNPGDSKDMKQSEPNYEGLSGNDQEKDSSQASKSSVKVPETHKAVLALRLEEKDGKIAVQTEKPESKASTDIAGQAVTINLVPVEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGHANSPKTKSSSSTPNSPVTSPALTSGHISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELISSGTGPNVPPRKICHKSAPTSPTATNVSSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKASDNATSKPTECPQAKAFSNSTERKRGSVAQKVQEFNSCLNRSQSSPQRSYSSNHSSPAKLQRTTQEPVAKTESTQEPQVVGGGSSTREKASTVLSQMVASIQPPQSPPETPQSGPKACSVEELYAVPPDASAAKTTPKGTPVRPKSLFMSQPSAEAEAPQTTESPTTKAQKDPLTKPVTTPPSKLVTNPQSDPPAPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKDREKGKLVGLDGTVIHMLPPPPVQRHRWFTEAKGDSSEKPAIVFMYRCDPAQGQLSMDQGKARAEQSAVTEKGGAEEGSLQDSEKKKRSHSSPSQIPKKILSHVTHEVTEDFSPRDPRTVAVKQDGGGCTSVTPALHLPELEREEEKEDISDPIDLNPCSATYSNLGQSRAAMIPPKHPRQPKGALDDVIAFGGKTDQEAPNALQPTPPPLPKKMIIRANTEPLSKDLQKSLETTLCVMANPTYDIDPSWDASSAGSSVSYELKGLDAESYDSLERPLYKERRVPSAANSISSLTTLSIKDRFSNSTESLSSRRGPSGRQGRSIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAIRQSLAVHFNIQQDCGHFLAEVPKRLLPWEDPDAAEEEEDGMEATEEVKGEADGKGPDLGSEAESSHKESQAAVSRKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDAYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGPTQGLGPAEPSPTPSCPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYTRADLPRIPLRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTASPSPAHRNTLLQNWLDIKRTLLMIKFAEKSLDREGGVTLEDWLCAQYLAFATPDSLSCVVRILQPR

Target ORF information:

RefSeq Version	XM_005222004.4
Organism	Bos taurus(cattle)
Definition	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222004.4

ORF Insert Sequence:

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCAGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATC 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEAK1 ( XM_003587568.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003587616.2 Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X9, mRNA.

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CloneID	OBa228456
Clone ID Related Accession (Same CDS sequence)	XM_005222004.4 , XM_003587568.5 , XM_005222010.4 , XM_024982035.1 , XM_024982037.1 , XM_005222005.4 , XM_015459262.2 , XM_010817120.3 , XM_015459261.2 , XM_024982036.1
Accession Version	XM_003587568.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003587568.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCAGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATC 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003587616.2
CDS	456..5699
Translation	MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMMVADGQSVCGELSIQEHCENKPVVIGWNRNRMALNQKPLNNNNEDDVEGFSHVPKPYGNNDSAKKISNNNNGLTEVLKEIAGLDTTPQIKENETNSRETFLGRINDCYKRSLERKLPPSCMMGGMKDTQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLLSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTVPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGPGSTQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGIAPGNPGDSKDMKQSEPNYEGLSGNDQEKDSSQASKSSVKVPETHKAVLALRLEEKDGKIAVQTEKPESKASTDIAGQAVTINLVPVEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGHANSPKTKSSSSTPNSPVTSPALTSGHISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELISSGTGPNVPPRKICHKSAPTSPTATNVSSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKASDNATSKPTECPQAKAFSNSTERKRGSVAQKVQEFNSCLNRSQSSPQRSYSSNHSSPAKLQRTTQEPVAKTESTQEPQVVGGGSSTREKASTVLSQMVASIQPPQSPPETPQSGPKACSVEELYAVPPDASAAKTTPKGTPVRPKSLFMSQPSAEAEAPQTTESPTTKAQKDPLTKPVTTPPSKLVTNPQSDPPAPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKDREKGKLVGLDGTVIHMLPPPPVQRHRWFTEAKGDSSEKPAIVFMYRCDPAQGQLSMDQGKARAEQSAVTEKGGAEEGSLQDSEKKKRSHSSPSQIPKKILSHVTHEVTEDFSPRDPRTVAVKQDGGGCTSVTPALHLPELEREEEKEDISDPIDLNPCSATYSNLGQSRAAMIPPKHPRQPKGALDDVIAFGGKTDQEAPNALQPTPPPLPKKMIIRANTEPLSKDLQKSLETTLCVMANPTYDIDPSWDASSAGSSVSYELKGLDAESYDSLERPLYKERRVPSAANSISSLTTLSIKDRFSNSTESLSSRRGPSGRQGRSIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAIRQSLAVHFNIQQDCGHFLAEVPKRLLPWEDPDAAEEEEDGMEATEEVKGEADGKGPDLGSEAESSHKESQAAVSRKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDAYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGPTQGLGPAEPSPTPSCPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYTRADLPRIPLRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTASPSPAHRNTLLQNWLDIKRTLLMIKFAEKSLDREGGVTLEDWLCAQYLAFATPDSLSCVVRILQPR

Target ORF information:

RefSeq Version	XM_003587568.5
Organism	Bos taurus(cattle)
Definition	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003587568.5

ORF Insert Sequence:

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCAGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATC 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEAK1 ( XM_005222010.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222067.2 Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa228456
Clone ID Related Accession (Same CDS sequence)	XM_005222004.4 , XM_003587568.5 , XM_005222010.4 , XM_024982035.1 , XM_024982037.1 , XM_005222005.4 , XM_015459262.2 , XM_010817120.3 , XM_015459261.2 , XM_024982036.1
Accession Version	XM_005222010.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222010.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCAGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATC 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222067.2
CDS	341..5584
Translation	MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMMVADGQSVCGELSIQEHCENKPVVIGWNRNRMALNQKPLNNNNEDDVEGFSHVPKPYGNNDSAKKISNNNNGLTEVLKEIAGLDTTPQIKENETNSRETFLGRINDCYKRSLERKLPPSCMMGGMKDTQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLLSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTVPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGPGSTQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGIAPGNPGDSKDMKQSEPNYEGLSGNDQEKDSSQASKSSVKVPETHKAVLALRLEEKDGKIAVQTEKPESKASTDIAGQAVTINLVPVEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGHANSPKTKSSSSTPNSPVTSPALTSGHISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELISSGTGPNVPPRKICHKSAPTSPTATNVSSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKASDNATSKPTECPQAKAFSNSTERKRGSVAQKVQEFNSCLNRSQSSPQRSYSSNHSSPAKLQRTTQEPVAKTESTQEPQVVGGGSSTREKASTVLSQMVASIQPPQSPPETPQSGPKACSVEELYAVPPDASAAKTTPKGTPVRPKSLFMSQPSAEAEAPQTTESPTTKAQKDPLTKPVTTPPSKLVTNPQSDPPAPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKDREKGKLVGLDGTVIHMLPPPPVQRHRWFTEAKGDSSEKPAIVFMYRCDPAQGQLSMDQGKARAEQSAVTEKGGAEEGSLQDSEKKKRSHSSPSQIPKKILSHVTHEVTEDFSPRDPRTVAVKQDGGGCTSVTPALHLPELEREEEKEDISDPIDLNPCSATYSNLGQSRAAMIPPKHPRQPKGALDDVIAFGGKTDQEAPNALQPTPPPLPKKMIIRANTEPLSKDLQKSLETTLCVMANPTYDIDPSWDASSAGSSVSYELKGLDAESYDSLERPLYKERRVPSAANSISSLTTLSIKDRFSNSTESLSSRRGPSGRQGRSIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAIRQSLAVHFNIQQDCGHFLAEVPKRLLPWEDPDAAEEEEDGMEATEEVKGEADGKGPDLGSEAESSHKESQAAVSRKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDAYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGPTQGLGPAEPSPTPSCPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYTRADLPRIPLRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTASPSPAHRNTLLQNWLDIKRTLLMIKFAEKSLDREGGVTLEDWLCAQYLAFATPDSLSCVVRILQPR

Target ORF information:

RefSeq Version	XM_005222010.4
Organism	Bos taurus(cattle)
Definition	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222010.4

ORF Insert Sequence:

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCAGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATC 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEAK1 ( XM_024982035.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837803.1 Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa228456
Clone ID Related Accession (Same CDS sequence)	XM_005222004.4 , XM_003587568.5 , XM_005222010.4 , XM_024982035.1 , XM_024982037.1 , XM_005222005.4 , XM_015459262.2 , XM_010817120.3 , XM_015459261.2 , XM_024982036.1
Accession Version	XM_024982035.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCAGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATC 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837803.1
CDS	655..5898
Translation	MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMMVADGQSVCGELSIQEHCENKPVVIGWNRNRMALNQKPLNNNNEDDVEGFSHVPKPYGNNDSAKKISNNNNGLTEVLKEIAGLDTTPQIKENETNSRETFLGRINDCYKRSLERKLPPSCMMGGMKDTQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLLSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTVPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGPGSTQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGIAPGNPGDSKDMKQSEPNYEGLSGNDQEKDSSQASKSSVKVPETHKAVLALRLEEKDGKIAVQTEKPESKASTDIAGQAVTINLVPVEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGHANSPKTKSSSSTPNSPVTSPALTSGHISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELISSGTGPNVPPRKICHKSAPTSPTATNVSSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKASDNATSKPTECPQAKAFSNSTERKRGSVAQKVQEFNSCLNRSQSSPQRSYSSNHSSPAKLQRTTQEPVAKTESTQEPQVVGGGSSTREKASTVLSQMVASIQPPQSPPETPQSGPKACSVEELYAVPPDASAAKTTPKGTPVRPKSLFMSQPSAEAEAPQTTESPTTKAQKDPLTKPVTTPPSKLVTNPQSDPPAPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKDREKGKLVGLDGTVIHMLPPPPVQRHRWFTEAKGDSSEKPAIVFMYRCDPAQGQLSMDQGKARAEQSAVTEKGGAEEGSLQDSEKKKRSHSSPSQIPKKILSHVTHEVTEDFSPRDPRTVAVKQDGGGCTSVTPALHLPELEREEEKEDISDPIDLNPCSATYSNLGQSRAAMIPPKHPRQPKGALDDVIAFGGKTDQEAPNALQPTPPPLPKKMIIRANTEPLSKDLQKSLETTLCVMANPTYDIDPSWDASSAGSSVSYELKGLDAESYDSLERPLYKERRVPSAANSISSLTTLSIKDRFSNSTESLSSRRGPSGRQGRSIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAIRQSLAVHFNIQQDCGHFLAEVPKRLLPWEDPDAAEEEEDGMEATEEVKGEADGKGPDLGSEAESSHKESQAAVSRKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDAYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGPTQGLGPAEPSPTPSCPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYTRADLPRIPLRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTASPSPAHRNTLLQNWLDIKRTLLMIKFAEKSLDREGGVTLEDWLCAQYLAFATPDSLSCVVRILQPR

Target ORF information:

RefSeq Version	XM_024982035.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982035.1

ORF Insert Sequence:

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCAGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATC 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEAK1 ( XM_024982037.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837805.1 Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X10, mRNA.

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CloneID	OBa228456
Clone ID Related Accession (Same CDS sequence)	XM_005222004.4 , XM_003587568.5 , XM_005222010.4 , XM_024982035.1 , XM_024982037.1 , XM_005222005.4 , XM_015459262.2 , XM_010817120.3 , XM_015459261.2 , XM_024982036.1
Accession Version	XM_024982037.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCAGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATC 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837805.1
CDS	357..5600
Translation	MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMMVADGQSVCGELSIQEHCENKPVVIGWNRNRMALNQKPLNNNNEDDVEGFSHVPKPYGNNDSAKKISNNNNGLTEVLKEIAGLDTTPQIKENETNSRETFLGRINDCYKRSLERKLPPSCMMGGMKDTQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLLSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTVPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGPGSTQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGIAPGNPGDSKDMKQSEPNYEGLSGNDQEKDSSQASKSSVKVPETHKAVLALRLEEKDGKIAVQTEKPESKASTDIAGQAVTINLVPVEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGHANSPKTKSSSSTPNSPVTSPALTSGHISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELISSGTGPNVPPRKICHKSAPTSPTATNVSSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKASDNATSKPTECPQAKAFSNSTERKRGSVAQKVQEFNSCLNRSQSSPQRSYSSNHSSPAKLQRTTQEPVAKTESTQEPQVVGGGSSTREKASTVLSQMVASIQPPQSPPETPQSGPKACSVEELYAVPPDASAAKTTPKGTPVRPKSLFMSQPSAEAEAPQTTESPTTKAQKDPLTKPVTTPPSKLVTNPQSDPPAPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKDREKGKLVGLDGTVIHMLPPPPVQRHRWFTEAKGDSSEKPAIVFMYRCDPAQGQLSMDQGKARAEQSAVTEKGGAEEGSLQDSEKKKRSHSSPSQIPKKILSHVTHEVTEDFSPRDPRTVAVKQDGGGCTSVTPALHLPELEREEEKEDISDPIDLNPCSATYSNLGQSRAAMIPPKHPRQPKGALDDVIAFGGKTDQEAPNALQPTPPPLPKKMIIRANTEPLSKDLQKSLETTLCVMANPTYDIDPSWDASSAGSSVSYELKGLDAESYDSLERPLYKERRVPSAANSISSLTTLSIKDRFSNSTESLSSRRGPSGRQGRSIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAIRQSLAVHFNIQQDCGHFLAEVPKRLLPWEDPDAAEEEEDGMEATEEVKGEADGKGPDLGSEAESSHKESQAAVSRKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDAYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGPTQGLGPAEPSPTPSCPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYTRADLPRIPLRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTASPSPAHRNTLLQNWLDIKRTLLMIKFAEKSLDREGGVTLEDWLCAQYLAFATPDSLSCVVRILQPR

Target ORF information:

RefSeq Version	XM_024982037.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982037.1

ORF Insert Sequence:

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCAGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATC 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEAK1 ( XM_005222005.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222062.2 Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X2, mRNA.

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CloneID	OBa228456
Clone ID Related Accession (Same CDS sequence)	XM_005222004.4 , XM_003587568.5 , XM_005222010.4 , XM_024982035.1 , XM_024982037.1 , XM_005222005.4 , XM_015459262.2 , XM_010817120.3 , XM_015459261.2 , XM_024982036.1
Accession Version	XM_005222005.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222005.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCAGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATC 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222062.2
CDS	641..5884
Translation	MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMMVADGQSVCGELSIQEHCENKPVVIGWNRNRMALNQKPLNNNNEDDVEGFSHVPKPYGNNDSAKKISNNNNGLTEVLKEIAGLDTTPQIKENETNSRETFLGRINDCYKRSLERKLPPSCMMGGMKDTQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLLSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTVPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGPGSTQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGIAPGNPGDSKDMKQSEPNYEGLSGNDQEKDSSQASKSSVKVPETHKAVLALRLEEKDGKIAVQTEKPESKASTDIAGQAVTINLVPVEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGHANSPKTKSSSSTPNSPVTSPALTSGHISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELISSGTGPNVPPRKICHKSAPTSPTATNVSSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKASDNATSKPTECPQAKAFSNSTERKRGSVAQKVQEFNSCLNRSQSSPQRSYSSNHSSPAKLQRTTQEPVAKTESTQEPQVVGGGSSTREKASTVLSQMVASIQPPQSPPETPQSGPKACSVEELYAVPPDASAAKTTPKGTPVRPKSLFMSQPSAEAEAPQTTESPTTKAQKDPLTKPVTTPPSKLVTNPQSDPPAPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKDREKGKLVGLDGTVIHMLPPPPVQRHRWFTEAKGDSSEKPAIVFMYRCDPAQGQLSMDQGKARAEQSAVTEKGGAEEGSLQDSEKKKRSHSSPSQIPKKILSHVTHEVTEDFSPRDPRTVAVKQDGGGCTSVTPALHLPELEREEEKEDISDPIDLNPCSATYSNLGQSRAAMIPPKHPRQPKGALDDVIAFGGKTDQEAPNALQPTPPPLPKKMIIRANTEPLSKDLQKSLETTLCVMANPTYDIDPSWDASSAGSSVSYELKGLDAESYDSLERPLYKERRVPSAANSISSLTTLSIKDRFSNSTESLSSRRGPSGRQGRSIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAIRQSLAVHFNIQQDCGHFLAEVPKRLLPWEDPDAAEEEEDGMEATEEVKGEADGKGPDLGSEAESSHKESQAAVSRKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDAYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGPTQGLGPAEPSPTPSCPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYTRADLPRIPLRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTASPSPAHRNTLLQNWLDIKRTLLMIKFAEKSLDREGGVTLEDWLCAQYLAFATPDSLSCVVRILQPR

Target ORF information:

RefSeq Version	XM_005222005.4
Organism	Bos taurus(cattle)
Definition	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222005.4

ORF Insert Sequence:

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCAGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATC 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEAK1 ( XM_015459262.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314748.2 Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X3, mRNA.

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CloneID	OBa228456
Clone ID Related Accession (Same CDS sequence)	XM_005222004.4 , XM_003587568.5 , XM_005222010.4 , XM_024982035.1 , XM_024982037.1 , XM_005222005.4 , XM_015459262.2 , XM_010817120.3 , XM_015459261.2 , XM_024982036.1
Accession Version	XM_015459262.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015459262.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCAGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATC 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314748.2
CDS	608..5851
Translation	MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMMVADGQSVCGELSIQEHCENKPVVIGWNRNRMALNQKPLNNNNEDDVEGFSHVPKPYGNNDSAKKISNNNNGLTEVLKEIAGLDTTPQIKENETNSRETFLGRINDCYKRSLERKLPPSCMMGGMKDTQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLLSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTVPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGPGSTQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGIAPGNPGDSKDMKQSEPNYEGLSGNDQEKDSSQASKSSVKVPETHKAVLALRLEEKDGKIAVQTEKPESKASTDIAGQAVTINLVPVEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGHANSPKTKSSSSTPNSPVTSPALTSGHISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELISSGTGPNVPPRKICHKSAPTSPTATNVSSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKASDNATSKPTECPQAKAFSNSTERKRGSVAQKVQEFNSCLNRSQSSPQRSYSSNHSSPAKLQRTTQEPVAKTESTQEPQVVGGGSSTREKASTVLSQMVASIQPPQSPPETPQSGPKACSVEELYAVPPDASAAKTTPKGTPVRPKSLFMSQPSAEAEAPQTTESPTTKAQKDPLTKPVTTPPSKLVTNPQSDPPAPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKDREKGKLVGLDGTVIHMLPPPPVQRHRWFTEAKGDSSEKPAIVFMYRCDPAQGQLSMDQGKARAEQSAVTEKGGAEEGSLQDSEKKKRSHSSPSQIPKKILSHVTHEVTEDFSPRDPRTVAVKQDGGGCTSVTPALHLPELEREEEKEDISDPIDLNPCSATYSNLGQSRAAMIPPKHPRQPKGALDDVIAFGGKTDQEAPNALQPTPPPLPKKMIIRANTEPLSKDLQKSLETTLCVMANPTYDIDPSWDASSAGSSVSYELKGLDAESYDSLERPLYKERRVPSAANSISSLTTLSIKDRFSNSTESLSSRRGPSGRQGRSIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAIRQSLAVHFNIQQDCGHFLAEVPKRLLPWEDPDAAEEEEDGMEATEEVKGEADGKGPDLGSEAESSHKESQAAVSRKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDAYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGPTQGLGPAEPSPTPSCPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYTRADLPRIPLRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTASPSPAHRNTLLQNWLDIKRTLLMIKFAEKSLDREGGVTLEDWLCAQYLAFATPDSLSCVVRILQPR

Target ORF information:

RefSeq Version	XM_015459262.2
Organism	Bos taurus(cattle)
Definition	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459262.2

ORF Insert Sequence:

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCAGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATC 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEAK1 ( XM_010817120.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815422.2 Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X4, mRNA.

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CloneID	OBa228456
Clone ID Related Accession (Same CDS sequence)	XM_005222004.4 , XM_003587568.5 , XM_005222010.4 , XM_024982035.1 , XM_024982037.1 , XM_005222005.4 , XM_015459262.2 , XM_010817120.3 , XM_015459261.2 , XM_024982036.1
Accession Version	XM_010817120.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817120.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCAGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATC 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815422.2
CDS	606..5849
Translation	MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMMVADGQSVCGELSIQEHCENKPVVIGWNRNRMALNQKPLNNNNEDDVEGFSHVPKPYGNNDSAKKISNNNNGLTEVLKEIAGLDTTPQIKENETNSRETFLGRINDCYKRSLERKLPPSCMMGGMKDTQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLLSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTVPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGPGSTQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGIAPGNPGDSKDMKQSEPNYEGLSGNDQEKDSSQASKSSVKVPETHKAVLALRLEEKDGKIAVQTEKPESKASTDIAGQAVTINLVPVEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGHANSPKTKSSSSTPNSPVTSPALTSGHISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELISSGTGPNVPPRKICHKSAPTSPTATNVSSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKASDNATSKPTECPQAKAFSNSTERKRGSVAQKVQEFNSCLNRSQSSPQRSYSSNHSSPAKLQRTTQEPVAKTESTQEPQVVGGGSSTREKASTVLSQMVASIQPPQSPPETPQSGPKACSVEELYAVPPDASAAKTTPKGTPVRPKSLFMSQPSAEAEAPQTTESPTTKAQKDPLTKPVTTPPSKLVTNPQSDPPAPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKDREKGKLVGLDGTVIHMLPPPPVQRHRWFTEAKGDSSEKPAIVFMYRCDPAQGQLSMDQGKARAEQSAVTEKGGAEEGSLQDSEKKKRSHSSPSQIPKKILSHVTHEVTEDFSPRDPRTVAVKQDGGGCTSVTPALHLPELEREEEKEDISDPIDLNPCSATYSNLGQSRAAMIPPKHPRQPKGALDDVIAFGGKTDQEAPNALQPTPPPLPKKMIIRANTEPLSKDLQKSLETTLCVMANPTYDIDPSWDASSAGSSVSYELKGLDAESYDSLERPLYKERRVPSAANSISSLTTLSIKDRFSNSTESLSSRRGPSGRQGRSIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAIRQSLAVHFNIQQDCGHFLAEVPKRLLPWEDPDAAEEEEDGMEATEEVKGEADGKGPDLGSEAESSHKESQAAVSRKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDAYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGPTQGLGPAEPSPTPSCPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYTRADLPRIPLRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTASPSPAHRNTLLQNWLDIKRTLLMIKFAEKSLDREGGVTLEDWLCAQYLAFATPDSLSCVVRILQPR

Target ORF information:

RefSeq Version	XM_010817120.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817120.3

ORF Insert Sequence:

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCAGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATC 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEAK1 ( XM_015459261.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314747.2 Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X5, mRNA.

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CloneID	OBa228456
Clone ID Related Accession (Same CDS sequence)	XM_005222004.4 , XM_003587568.5 , XM_005222010.4 , XM_024982035.1 , XM_024982037.1 , XM_005222005.4 , XM_015459262.2 , XM_010817120.3 , XM_015459261.2 , XM_024982036.1
Accession Version	XM_015459261.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015459261.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCAGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATC 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314747.2
CDS	599..5842
Translation	MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMMVADGQSVCGELSIQEHCENKPVVIGWNRNRMALNQKPLNNNNEDDVEGFSHVPKPYGNNDSAKKISNNNNGLTEVLKEIAGLDTTPQIKENETNSRETFLGRINDCYKRSLERKLPPSCMMGGMKDTQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLLSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTVPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGPGSTQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGIAPGNPGDSKDMKQSEPNYEGLSGNDQEKDSSQASKSSVKVPETHKAVLALRLEEKDGKIAVQTEKPESKASTDIAGQAVTINLVPVEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGHANSPKTKSSSSTPNSPVTSPALTSGHISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELISSGTGPNVPPRKICHKSAPTSPTATNVSSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKASDNATSKPTECPQAKAFSNSTERKRGSVAQKVQEFNSCLNRSQSSPQRSYSSNHSSPAKLQRTTQEPVAKTESTQEPQVVGGGSSTREKASTVLSQMVASIQPPQSPPETPQSGPKACSVEELYAVPPDASAAKTTPKGTPVRPKSLFMSQPSAEAEAPQTTESPTTKAQKDPLTKPVTTPPSKLVTNPQSDPPAPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKDREKGKLVGLDGTVIHMLPPPPVQRHRWFTEAKGDSSEKPAIVFMYRCDPAQGQLSMDQGKARAEQSAVTEKGGAEEGSLQDSEKKKRSHSSPSQIPKKILSHVTHEVTEDFSPRDPRTVAVKQDGGGCTSVTPALHLPELEREEEKEDISDPIDLNPCSATYSNLGQSRAAMIPPKHPRQPKGALDDVIAFGGKTDQEAPNALQPTPPPLPKKMIIRANTEPLSKDLQKSLETTLCVMANPTYDIDPSWDASSAGSSVSYELKGLDAESYDSLERPLYKERRVPSAANSISSLTTLSIKDRFSNSTESLSSRRGPSGRQGRSIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAIRQSLAVHFNIQQDCGHFLAEVPKRLLPWEDPDAAEEEEDGMEATEEVKGEADGKGPDLGSEAESSHKESQAAVSRKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDAYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGPTQGLGPAEPSPTPSCPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYTRADLPRIPLRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTASPSPAHRNTLLQNWLDIKRTLLMIKFAEKSLDREGGVTLEDWLCAQYLAFATPDSLSCVVRILQPR

Target ORF information:

RefSeq Version	XM_015459261.2
Organism	Bos taurus(cattle)
Definition	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459261.2

ORF Insert Sequence:

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCAGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATC 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEAK1 ( XM_024982036.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837804.1 Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X7, mRNA.

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CloneID	OBa228456
Clone ID Related Accession (Same CDS sequence)	XM_005222004.4 , XM_003587568.5 , XM_005222010.4 , XM_024982035.1 , XM_024982037.1 , XM_005222005.4 , XM_015459262.2 , XM_010817120.3 , XM_015459261.2 , XM_024982036.1
Accession Version	XM_024982036.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCAGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATC 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837804.1
CDS	741..5984
Translation	MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMMVADGQSVCGELSIQEHCENKPVVIGWNRNRMALNQKPLNNNNEDDVEGFSHVPKPYGNNDSAKKISNNNNGLTEVLKEIAGLDTTPQIKENETNSRETFLGRINDCYKRSLERKLPPSCMMGGMKDTQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLLSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTVPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGPGSTQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGIAPGNPGDSKDMKQSEPNYEGLSGNDQEKDSSQASKSSVKVPETHKAVLALRLEEKDGKIAVQTEKPESKASTDIAGQAVTINLVPVEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGHANSPKTKSSSSTPNSPVTSPALTSGHISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELISSGTGPNVPPRKICHKSAPTSPTATNVSSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKASDNATSKPTECPQAKAFSNSTERKRGSVAQKVQEFNSCLNRSQSSPQRSYSSNHSSPAKLQRTTQEPVAKTESTQEPQVVGGGSSTREKASTVLSQMVASIQPPQSPPETPQSGPKACSVEELYAVPPDASAAKTTPKGTPVRPKSLFMSQPSAEAEAPQTTESPTTKAQKDPLTKPVTTPPSKLVTNPQSDPPAPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKDREKGKLVGLDGTVIHMLPPPPVQRHRWFTEAKGDSSEKPAIVFMYRCDPAQGQLSMDQGKARAEQSAVTEKGGAEEGSLQDSEKKKRSHSSPSQIPKKILSHVTHEVTEDFSPRDPRTVAVKQDGGGCTSVTPALHLPELEREEEKEDISDPIDLNPCSATYSNLGQSRAAMIPPKHPRQPKGALDDVIAFGGKTDQEAPNALQPTPPPLPKKMIIRANTEPLSKDLQKSLETTLCVMANPTYDIDPSWDASSAGSSVSYELKGLDAESYDSLERPLYKERRVPSAANSISSLTTLSIKDRFSNSTESLSSRRGPSGRQGRSIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAIRQSLAVHFNIQQDCGHFLAEVPKRLLPWEDPDAAEEEEDGMEATEEVKGEADGKGPDLGSEAESSHKESQAAVSRKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDAYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGPTQGLGPAEPSPTPSCPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYTRADLPRIPLRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTASPSPAHRNTLLQNWLDIKRTLLMIKFAEKSLDREGGVTLEDWLCAQYLAFATPDSLSCVVRILQPR

Target ORF information:

RefSeq Version	XM_024982036.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982036.1

ORF Insert Sequence:

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCAGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATC 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEAK1 ( XM_015468519.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324005.1 Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X7, mRNA.

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CloneID	OBa10769
Clone ID Related Accession (Same CDS sequence)	XM_015468519.1 , XM_015459261.1 , XM_010817120.2 , XM_015459262.1 , XM_005222010.3 , XM_003587568.4 , XM_015468514.1 , XM_015468516.1 , XM_015468518.1 , XM_010798541.2 , XM_015468517.1 , XM_015468520.1 , XM_005222009.3 , XM_005222005.3 , XM_005222004.3 , XM_015468515.1
Accession Version	XM_015468519.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646534.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324005.1
CDS	326..5569
Translation	MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMMVADGQSVCGELSIQEHCENKPVVIGWNRNRMALNQKPLNNNNEDDVEGFSHVPKPYGNNDSAKKISNNNNGLTEVLKEIAGLDTTPQIKENETNSRETFLGRINDCYKRSLERKLPPSCMMGGMKDTQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLLSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTVPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGPGSTQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGIAPGNPGDSKDMKQSEPNYEGLSGNDQEKDSSQASKSSVKVPETHKAVLALRLEEKDGKIAVQTEKPESKASTDIAGQAVTINLVPVEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGHANSPKTKSSSSTPNSPVTSPALTSGHISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELISSGTGPNVPPRKICHKSAPTSPTATNVSSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKASDNATSKPTECPQAKAFSNSTERKRGSVAQKVQEFNSCLNRSQSSPQRSYSSNHSSPAKLQRTTQEPVAKTESTQEPQVVGGGSSTREKASTVLSQMVASIQPPQSPPETPQSGPKACSVEELYAVPPDAGAAKTTPKGTPVRPKSLFMSQPSAEAEAPQTTESPTTKAQKDPLTKPVTTPPSKLVTNPQSDPPAPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKDREKGKLVGLDGTVIHMLPPPPVQRHRWFTEAKGDSSEKPAIVFMYRCDPAQGQLSMDQGKARAEQSAVTEKGGAEEGSLQDSEKKKRSHSSPSQIPKKILSHVTHEVTEDFSPRDPRTVAVKQDGGGCTSVTPALHLPELEREEEKEDISDPMDLNPCSATYSNLGQSRAAMIPPKHPRQPKGALDDVIAFGGKTDQEAPNALQPTPPPLPKKMIIRANTEPLSKDLQKSLETTLCVMANPTYDIDPSWDASSAGSSVSYELKGLDAESYDSLERPLYKERRVPSAANSISSLTTLSIKDRFSNSTESLSSRRGPSGRQGRSIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAIRQSLAVHFNIQQDCGHFLAEVPKRLLPWEDPDAAEEEEDGMEATEEVKGEADGKGPDLGSEAESSHKESQAAVSRKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDAYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGPTQGLGPAEPSPTPSCPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYTRADLPRIPLRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTASPSPAHRNTLLQNWLDIKRTLLMIKFAEKSLDREGGVTLEDWLCAQYLAFATPDSLSCVVRILQPR

Target ORF information:

RefSeq Version	XM_015468519.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468519.1

ORF Insert Sequence:

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEAK1 ( XM_015459261.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314747.1 Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X3, mRNA.

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CloneID	OBa10769
Clone ID Related Accession (Same CDS sequence)	XM_015468519.1 , XM_015459261.1 , XM_010817120.2 , XM_015459262.1 , XM_005222010.3 , XM_003587568.4 , XM_015468514.1 , XM_015468516.1 , XM_015468518.1 , XM_010798541.2 , XM_015468517.1 , XM_015468520.1 , XM_005222009.3 , XM_005222005.3 , XM_005222004.3 , XM_015468515.1
Accession Version	XM_015459261.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104535.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314747.1
CDS	596..5839
Translation	MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMMVADGQSVCGELSIQEHCENKPVVIGWNRNRMALNQKPLNNNNEDDVEGFSHVPKPYGNNDSAKKISNNNNGLTEVLKEIAGLDTTPQIKENETNSRETFLGRINDCYKRSLERKLPPSCMMGGMKDTQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLLSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTVPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGPGSTQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGIAPGNPGDSKDMKQSEPNYEGLSGNDQEKDSSQASKSSVKVPETHKAVLALRLEEKDGKIAVQTEKPESKASTDIAGQAVTINLVPVEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGHANSPKTKSSSSTPNSPVTSPALTSGHISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELISSGTGPNVPPRKICHKSAPTSPTATNVSSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKASDNATSKPTECPQAKAFSNSTERKRGSVAQKVQEFNSCLNRSQSSPQRSYSSNHSSPAKLQRTTQEPVAKTESTQEPQVVGGGSSTREKASTVLSQMVASIQPPQSPPETPQSGPKACSVEELYAVPPDAGAAKTTPKGTPVRPKSLFMSQPSAEAEAPQTTESPTTKAQKDPLTKPVTTPPSKLVTNPQSDPPAPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKDREKGKLVGLDGTVIHMLPPPPVQRHRWFTEAKGDSSEKPAIVFMYRCDPAQGQLSMDQGKARAEQSAVTEKGGAEEGSLQDSEKKKRSHSSPSQIPKKILSHVTHEVTEDFSPRDPRTVAVKQDGGGCTSVTPALHLPELEREEEKEDISDPMDLNPCSATYSNLGQSRAAMIPPKHPRQPKGALDDVIAFGGKTDQEAPNALQPTPPPLPKKMIIRANTEPLSKDLQKSLETTLCVMANPTYDIDPSWDASSAGSSVSYELKGLDAESYDSLERPLYKERRVPSAANSISSLTTLSIKDRFSNSTESLSSRRGPSGRQGRSIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAIRQSLAVHFNIQQDCGHFLAEVPKRLLPWEDPDAAEEEEDGMEATEEVKGEADGKGPDLGSEAESSHKESQAAVSRKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDAYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGPTQGLGPAEPSPTPSCPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYTRADLPRIPLRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTASPSPAHRNTLLQNWLDIKRTLLMIKFAEKSLDREGGVTLEDWLCAQYLAFATPDSLSCVVRILQPR

Target ORF information:

RefSeq Version	XM_015459261.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459261.1

ORF Insert Sequence:

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEAK1 ( XM_010817120.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815422.1 Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X4, mRNA.

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CloneID	OBa10769
Clone ID Related Accession (Same CDS sequence)	XM_015468519.1 , XM_015459261.1 , XM_010817120.2 , XM_015459262.1 , XM_005222010.3 , XM_003587568.4 , XM_015468514.1 , XM_015468516.1 , XM_015468518.1 , XM_010798541.2 , XM_015468517.1 , XM_015468520.1 , XM_005222009.3 , XM_005222005.3 , XM_005222004.3 , XM_015468515.1
Accession Version	XM_010817120.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104535.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817120.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815422.1
CDS	603..5846
Translation	MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMMVADGQSVCGELSIQEHCENKPVVIGWNRNRMALNQKPLNNNNEDDVEGFSHVPKPYGNNDSAKKISNNNNGLTEVLKEIAGLDTTPQIKENETNSRETFLGRINDCYKRSLERKLPPSCMMGGMKDTQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLLSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTVPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGPGSTQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGIAPGNPGDSKDMKQSEPNYEGLSGNDQEKDSSQASKSSVKVPETHKAVLALRLEEKDGKIAVQTEKPESKASTDIAGQAVTINLVPVEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGHANSPKTKSSSSTPNSPVTSPALTSGHISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELISSGTGPNVPPRKICHKSAPTSPTATNVSSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKASDNATSKPTECPQAKAFSNSTERKRGSVAQKVQEFNSCLNRSQSSPQRSYSSNHSSPAKLQRTTQEPVAKTESTQEPQVVGGGSSTREKASTVLSQMVASIQPPQSPPETPQSGPKACSVEELYAVPPDAGAAKTTPKGTPVRPKSLFMSQPSAEAEAPQTTESPTTKAQKDPLTKPVTTPPSKLVTNPQSDPPAPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKDREKGKLVGLDGTVIHMLPPPPVQRHRWFTEAKGDSSEKPAIVFMYRCDPAQGQLSMDQGKARAEQSAVTEKGGAEEGSLQDSEKKKRSHSSPSQIPKKILSHVTHEVTEDFSPRDPRTVAVKQDGGGCTSVTPALHLPELEREEEKEDISDPMDLNPCSATYSNLGQSRAAMIPPKHPRQPKGALDDVIAFGGKTDQEAPNALQPTPPPLPKKMIIRANTEPLSKDLQKSLETTLCVMANPTYDIDPSWDASSAGSSVSYELKGLDAESYDSLERPLYKERRVPSAANSISSLTTLSIKDRFSNSTESLSSRRGPSGRQGRSIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAIRQSLAVHFNIQQDCGHFLAEVPKRLLPWEDPDAAEEEEDGMEATEEVKGEADGKGPDLGSEAESSHKESQAAVSRKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDAYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGPTQGLGPAEPSPTPSCPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYTRADLPRIPLRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTASPSPAHRNTLLQNWLDIKRTLLMIKFAEKSLDREGGVTLEDWLCAQYLAFATPDSLSCVVRILQPR

Target ORF information:

RefSeq Version	XM_010817120.2
Organism	Bos taurus(cattle)
Definition	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817120.2

ORF Insert Sequence:

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEAK1 ( XM_015459262.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314748.1 Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X5, mRNA.

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CloneID	OBa10769
Clone ID Related Accession (Same CDS sequence)	XM_015468519.1 , XM_015459261.1 , XM_010817120.2 , XM_015459262.1 , XM_005222010.3 , XM_003587568.4 , XM_015468514.1 , XM_015468516.1 , XM_015468518.1 , XM_010798541.2 , XM_015468517.1 , XM_015468520.1 , XM_005222009.3 , XM_005222005.3 , XM_005222004.3 , XM_015468515.1
Accession Version	XM_015459262.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104535.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314748.1
CDS	605..5848
Translation	MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMMVADGQSVCGELSIQEHCENKPVVIGWNRNRMALNQKPLNNNNEDDVEGFSHVPKPYGNNDSAKKISNNNNGLTEVLKEIAGLDTTPQIKENETNSRETFLGRINDCYKRSLERKLPPSCMMGGMKDTQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLLSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTVPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGPGSTQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGIAPGNPGDSKDMKQSEPNYEGLSGNDQEKDSSQASKSSVKVPETHKAVLALRLEEKDGKIAVQTEKPESKASTDIAGQAVTINLVPVEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGHANSPKTKSSSSTPNSPVTSPALTSGHISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELISSGTGPNVPPRKICHKSAPTSPTATNVSSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKASDNATSKPTECPQAKAFSNSTERKRGSVAQKVQEFNSCLNRSQSSPQRSYSSNHSSPAKLQRTTQEPVAKTESTQEPQVVGGGSSTREKASTVLSQMVASIQPPQSPPETPQSGPKACSVEELYAVPPDAGAAKTTPKGTPVRPKSLFMSQPSAEAEAPQTTESPTTKAQKDPLTKPVTTPPSKLVTNPQSDPPAPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKDREKGKLVGLDGTVIHMLPPPPVQRHRWFTEAKGDSSEKPAIVFMYRCDPAQGQLSMDQGKARAEQSAVTEKGGAEEGSLQDSEKKKRSHSSPSQIPKKILSHVTHEVTEDFSPRDPRTVAVKQDGGGCTSVTPALHLPELEREEEKEDISDPMDLNPCSATYSNLGQSRAAMIPPKHPRQPKGALDDVIAFGGKTDQEAPNALQPTPPPLPKKMIIRANTEPLSKDLQKSLETTLCVMANPTYDIDPSWDASSAGSSVSYELKGLDAESYDSLERPLYKERRVPSAANSISSLTTLSIKDRFSNSTESLSSRRGPSGRQGRSIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAIRQSLAVHFNIQQDCGHFLAEVPKRLLPWEDPDAAEEEEDGMEATEEVKGEADGKGPDLGSEAESSHKESQAAVSRKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDAYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGPTQGLGPAEPSPTPSCPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYTRADLPRIPLRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTASPSPAHRNTLLQNWLDIKRTLLMIKFAEKSLDREGGVTLEDWLCAQYLAFATPDSLSCVVRILQPR

Target ORF information:

RefSeq Version	XM_015459262.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459262.1

ORF Insert Sequence:

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEAK1 ( XM_005222010.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222067.1 Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X7, mRNA.

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CloneID	OBa10769
Clone ID Related Accession (Same CDS sequence)	XM_015468519.1 , XM_015459261.1 , XM_010817120.2 , XM_015459262.1 , XM_005222010.3 , XM_003587568.4 , XM_015468514.1 , XM_015468516.1 , XM_015468518.1 , XM_010798541.2 , XM_015468517.1 , XM_015468520.1 , XM_005222009.3 , XM_005222005.3 , XM_005222004.3 , XM_015468515.1
Accession Version	XM_005222010.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104535.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222010.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222067.1
CDS	326..5569
Translation	MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMMVADGQSVCGELSIQEHCENKPVVIGWNRNRMALNQKPLNNNNEDDVEGFSHVPKPYGNNDSAKKISNNNNGLTEVLKEIAGLDTTPQIKENETNSRETFLGRINDCYKRSLERKLPPSCMMGGMKDTQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLLSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTVPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGPGSTQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGIAPGNPGDSKDMKQSEPNYEGLSGNDQEKDSSQASKSSVKVPETHKAVLALRLEEKDGKIAVQTEKPESKASTDIAGQAVTINLVPVEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGHANSPKTKSSSSTPNSPVTSPALTSGHISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELISSGTGPNVPPRKICHKSAPTSPTATNVSSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKASDNATSKPTECPQAKAFSNSTERKRGSVAQKVQEFNSCLNRSQSSPQRSYSSNHSSPAKLQRTTQEPVAKTESTQEPQVVGGGSSTREKASTVLSQMVASIQPPQSPPETPQSGPKACSVEELYAVPPDAGAAKTTPKGTPVRPKSLFMSQPSAEAEAPQTTESPTTKAQKDPLTKPVTTPPSKLVTNPQSDPPAPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKDREKGKLVGLDGTVIHMLPPPPVQRHRWFTEAKGDSSEKPAIVFMYRCDPAQGQLSMDQGKARAEQSAVTEKGGAEEGSLQDSEKKKRSHSSPSQIPKKILSHVTHEVTEDFSPRDPRTVAVKQDGGGCTSVTPALHLPELEREEEKEDISDPMDLNPCSATYSNLGQSRAAMIPPKHPRQPKGALDDVIAFGGKTDQEAPNALQPTPPPLPKKMIIRANTEPLSKDLQKSLETTLCVMANPTYDIDPSWDASSAGSSVSYELKGLDAESYDSLERPLYKERRVPSAANSISSLTTLSIKDRFSNSTESLSSRRGPSGRQGRSIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAIRQSLAVHFNIQQDCGHFLAEVPKRLLPWEDPDAAEEEEDGMEATEEVKGEADGKGPDLGSEAESSHKESQAAVSRKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDAYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGPTQGLGPAEPSPTPSCPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYTRADLPRIPLRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTASPSPAHRNTLLQNWLDIKRTLLMIKFAEKSLDREGGVTLEDWLCAQYLAFATPDSLSCVVRILQPR

Target ORF information:

RefSeq Version	XM_005222010.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222010.3

ORF Insert Sequence:

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEAK1 ( XM_003587568.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003587616.1 Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X8, mRNA.

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CloneID	OBa10769
Clone ID Related Accession (Same CDS sequence)	XM_015468519.1 , XM_015459261.1 , XM_010817120.2 , XM_015459262.1 , XM_005222010.3 , XM_003587568.4 , XM_015468514.1 , XM_015468516.1 , XM_015468518.1 , XM_010798541.2 , XM_015468517.1 , XM_015468520.1 , XM_005222009.3 , XM_005222005.3 , XM_005222004.3 , XM_015468515.1
Accession Version	XM_003587568.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104535.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003587568.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003587616.1
CDS	453..5696
Translation	MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMMVADGQSVCGELSIQEHCENKPVVIGWNRNRMALNQKPLNNNNEDDVEGFSHVPKPYGNNDSAKKISNNNNGLTEVLKEIAGLDTTPQIKENETNSRETFLGRINDCYKRSLERKLPPSCMMGGMKDTQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLLSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTVPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGPGSTQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGIAPGNPGDSKDMKQSEPNYEGLSGNDQEKDSSQASKSSVKVPETHKAVLALRLEEKDGKIAVQTEKPESKASTDIAGQAVTINLVPVEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGHANSPKTKSSSSTPNSPVTSPALTSGHISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELISSGTGPNVPPRKICHKSAPTSPTATNVSSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKASDNATSKPTECPQAKAFSNSTERKRGSVAQKVQEFNSCLNRSQSSPQRSYSSNHSSPAKLQRTTQEPVAKTESTQEPQVVGGGSSTREKASTVLSQMVASIQPPQSPPETPQSGPKACSVEELYAVPPDAGAAKTTPKGTPVRPKSLFMSQPSAEAEAPQTTESPTTKAQKDPLTKPVTTPPSKLVTNPQSDPPAPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKDREKGKLVGLDGTVIHMLPPPPVQRHRWFTEAKGDSSEKPAIVFMYRCDPAQGQLSMDQGKARAEQSAVTEKGGAEEGSLQDSEKKKRSHSSPSQIPKKILSHVTHEVTEDFSPRDPRTVAVKQDGGGCTSVTPALHLPELEREEEKEDISDPMDLNPCSATYSNLGQSRAAMIPPKHPRQPKGALDDVIAFGGKTDQEAPNALQPTPPPLPKKMIIRANTEPLSKDLQKSLETTLCVMANPTYDIDPSWDASSAGSSVSYELKGLDAESYDSLERPLYKERRVPSAANSISSLTTLSIKDRFSNSTESLSSRRGPSGRQGRSIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAIRQSLAVHFNIQQDCGHFLAEVPKRLLPWEDPDAAEEEEDGMEATEEVKGEADGKGPDLGSEAESSHKESQAAVSRKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDAYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGPTQGLGPAEPSPTPSCPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYTRADLPRIPLRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTASPSPAHRNTLLQNWLDIKRTLLMIKFAEKSLDREGGVTLEDWLCAQYLAFATPDSLSCVVRILQPR

Target ORF information:

RefSeq Version	XM_003587568.4
Organism	Bos taurus(cattle)
Definition	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003587568.4

ORF Insert Sequence:

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEAK1 ( XM_015468514.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324000.1 Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X2, mRNA.

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CloneID	OBa10769
Clone ID Related Accession (Same CDS sequence)	XM_015468519.1 , XM_015459261.1 , XM_010817120.2 , XM_015459262.1 , XM_005222010.3 , XM_003587568.4 , XM_015468514.1 , XM_015468516.1 , XM_015468518.1 , XM_010798541.2 , XM_015468517.1 , XM_015468520.1 , XM_005222009.3 , XM_005222005.3 , XM_005222004.3 , XM_015468515.1
Accession Version	XM_015468514.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646534.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324000.1
CDS	638..5881
Translation	MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMMVADGQSVCGELSIQEHCENKPVVIGWNRNRMALNQKPLNNNNEDDVEGFSHVPKPYGNNDSAKKISNNNNGLTEVLKEIAGLDTTPQIKENETNSRETFLGRINDCYKRSLERKLPPSCMMGGMKDTQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLLSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTVPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGPGSTQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGIAPGNPGDSKDMKQSEPNYEGLSGNDQEKDSSQASKSSVKVPETHKAVLALRLEEKDGKIAVQTEKPESKASTDIAGQAVTINLVPVEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGHANSPKTKSSSSTPNSPVTSPALTSGHISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELISSGTGPNVPPRKICHKSAPTSPTATNVSSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKASDNATSKPTECPQAKAFSNSTERKRGSVAQKVQEFNSCLNRSQSSPQRSYSSNHSSPAKLQRTTQEPVAKTESTQEPQVVGGGSSTREKASTVLSQMVASIQPPQSPPETPQSGPKACSVEELYAVPPDAGAAKTTPKGTPVRPKSLFMSQPSAEAEAPQTTESPTTKAQKDPLTKPVTTPPSKLVTNPQSDPPAPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKDREKGKLVGLDGTVIHMLPPPPVQRHRWFTEAKGDSSEKPAIVFMYRCDPAQGQLSMDQGKARAEQSAVTEKGGAEEGSLQDSEKKKRSHSSPSQIPKKILSHVTHEVTEDFSPRDPRTVAVKQDGGGCTSVTPALHLPELEREEEKEDISDPMDLNPCSATYSNLGQSRAAMIPPKHPRQPKGALDDVIAFGGKTDQEAPNALQPTPPPLPKKMIIRANTEPLSKDLQKSLETTLCVMANPTYDIDPSWDASSAGSSVSYELKGLDAESYDSLERPLYKERRVPSAANSISSLTTLSIKDRFSNSTESLSSRRGPSGRQGRSIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAIRQSLAVHFNIQQDCGHFLAEVPKRLLPWEDPDAAEEEEDGMEATEEVKGEADGKGPDLGSEAESSHKESQAAVSRKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDAYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGPTQGLGPAEPSPTPSCPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYTRADLPRIPLRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTASPSPAHRNTLLQNWLDIKRTLLMIKFAEKSLDREGGVTLEDWLCAQYLAFATPDSLSCVVRILQPR

Target ORF information:

RefSeq Version	XM_015468514.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468514.1

ORF Insert Sequence:

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEAK1 ( XM_015468516.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324002.1 Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X4, mRNA.

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CloneID	OBa10769
Clone ID Related Accession (Same CDS sequence)	XM_015468519.1 , XM_015459261.1 , XM_010817120.2 , XM_015459262.1 , XM_005222010.3 , XM_003587568.4 , XM_015468514.1 , XM_015468516.1 , XM_015468518.1 , XM_010798541.2 , XM_015468517.1 , XM_015468520.1 , XM_005222009.3 , XM_005222005.3 , XM_005222004.3 , XM_015468515.1
Accession Version	XM_015468516.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646534.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324002.1
CDS	603..5846
Translation	MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMMVADGQSVCGELSIQEHCENKPVVIGWNRNRMALNQKPLNNNNEDDVEGFSHVPKPYGNNDSAKKISNNNNGLTEVLKEIAGLDTTPQIKENETNSRETFLGRINDCYKRSLERKLPPSCMMGGMKDTQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLLSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTVPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGPGSTQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGIAPGNPGDSKDMKQSEPNYEGLSGNDQEKDSSQASKSSVKVPETHKAVLALRLEEKDGKIAVQTEKPESKASTDIAGQAVTINLVPVEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGHANSPKTKSSSSTPNSPVTSPALTSGHISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELISSGTGPNVPPRKICHKSAPTSPTATNVSSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKASDNATSKPTECPQAKAFSNSTERKRGSVAQKVQEFNSCLNRSQSSPQRSYSSNHSSPAKLQRTTQEPVAKTESTQEPQVVGGGSSTREKASTVLSQMVASIQPPQSPPETPQSGPKACSVEELYAVPPDAGAAKTTPKGTPVRPKSLFMSQPSAEAEAPQTTESPTTKAQKDPLTKPVTTPPSKLVTNPQSDPPAPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKDREKGKLVGLDGTVIHMLPPPPVQRHRWFTEAKGDSSEKPAIVFMYRCDPAQGQLSMDQGKARAEQSAVTEKGGAEEGSLQDSEKKKRSHSSPSQIPKKILSHVTHEVTEDFSPRDPRTVAVKQDGGGCTSVTPALHLPELEREEEKEDISDPMDLNPCSATYSNLGQSRAAMIPPKHPRQPKGALDDVIAFGGKTDQEAPNALQPTPPPLPKKMIIRANTEPLSKDLQKSLETTLCVMANPTYDIDPSWDASSAGSSVSYELKGLDAESYDSLERPLYKERRVPSAANSISSLTTLSIKDRFSNSTESLSSRRGPSGRQGRSIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAIRQSLAVHFNIQQDCGHFLAEVPKRLLPWEDPDAAEEEEDGMEATEEVKGEADGKGPDLGSEAESSHKESQAAVSRKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDAYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGPTQGLGPAEPSPTPSCPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYTRADLPRIPLRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTASPSPAHRNTLLQNWLDIKRTLLMIKFAEKSLDREGGVTLEDWLCAQYLAFATPDSLSCVVRILQPR

Target ORF information:

RefSeq Version	XM_015468516.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468516.1

ORF Insert Sequence:

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEAK1 ( XM_015468518.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324004.1 Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X6, mRNA.

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CloneID	OBa10769
Clone ID Related Accession (Same CDS sequence)	XM_015468519.1 , XM_015459261.1 , XM_010817120.2 , XM_015459262.1 , XM_005222010.3 , XM_003587568.4 , XM_015468514.1 , XM_015468516.1 , XM_015468518.1 , XM_010798541.2 , XM_015468517.1 , XM_015468520.1 , XM_005222009.3 , XM_005222005.3 , XM_005222004.3 , XM_015468515.1
Accession Version	XM_015468518.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646534.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324004.1
CDS	657..5900
Translation	MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMMVADGQSVCGELSIQEHCENKPVVIGWNRNRMALNQKPLNNNNEDDVEGFSHVPKPYGNNDSAKKISNNNNGLTEVLKEIAGLDTTPQIKENETNSRETFLGRINDCYKRSLERKLPPSCMMGGMKDTQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLLSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTVPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGPGSTQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGIAPGNPGDSKDMKQSEPNYEGLSGNDQEKDSSQASKSSVKVPETHKAVLALRLEEKDGKIAVQTEKPESKASTDIAGQAVTINLVPVEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGHANSPKTKSSSSTPNSPVTSPALTSGHISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELISSGTGPNVPPRKICHKSAPTSPTATNVSSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKASDNATSKPTECPQAKAFSNSTERKRGSVAQKVQEFNSCLNRSQSSPQRSYSSNHSSPAKLQRTTQEPVAKTESTQEPQVVGGGSSTREKASTVLSQMVASIQPPQSPPETPQSGPKACSVEELYAVPPDAGAAKTTPKGTPVRPKSLFMSQPSAEAEAPQTTESPTTKAQKDPLTKPVTTPPSKLVTNPQSDPPAPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKDREKGKLVGLDGTVIHMLPPPPVQRHRWFTEAKGDSSEKPAIVFMYRCDPAQGQLSMDQGKARAEQSAVTEKGGAEEGSLQDSEKKKRSHSSPSQIPKKILSHVTHEVTEDFSPRDPRTVAVKQDGGGCTSVTPALHLPELEREEEKEDISDPMDLNPCSATYSNLGQSRAAMIPPKHPRQPKGALDDVIAFGGKTDQEAPNALQPTPPPLPKKMIIRANTEPLSKDLQKSLETTLCVMANPTYDIDPSWDASSAGSSVSYELKGLDAESYDSLERPLYKERRVPSAANSISSLTTLSIKDRFSNSTESLSSRRGPSGRQGRSIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAIRQSLAVHFNIQQDCGHFLAEVPKRLLPWEDPDAAEEEEDGMEATEEVKGEADGKGPDLGSEAESSHKESQAAVSRKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDAYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGPTQGLGPAEPSPTPSCPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYTRADLPRIPLRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTASPSPAHRNTLLQNWLDIKRTLLMIKFAEKSLDREGGVTLEDWLCAQYLAFATPDSLSCVVRILQPR

Target ORF information:

RefSeq Version	XM_015468518.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468518.1

ORF Insert Sequence:

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEAK1 ( XM_010798541.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010796843.1 Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X1, mRNA.

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CloneID	OBa10769
Clone ID Related Accession (Same CDS sequence)	XM_015468519.1 , XM_015459261.1 , XM_010817120.2 , XM_015459262.1 , XM_005222010.3 , XM_003587568.4 , XM_015468514.1 , XM_015468516.1 , XM_015468518.1 , XM_010798541.2 , XM_015468517.1 , XM_015468520.1 , XM_005222009.3 , XM_005222005.3 , XM_005222004.3 , XM_015468515.1
Accession Version	XM_010798541.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646534.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798541.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010796843.1
CDS	517..5760
Translation	MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMMVADGQSVCGELSIQEHCENKPVVIGWNRNRMALNQKPLNNNNEDDVEGFSHVPKPYGNNDSAKKISNNNNGLTEVLKEIAGLDTTPQIKENETNSRETFLGRINDCYKRSLERKLPPSCMMGGMKDTQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLLSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTVPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGPGSTQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGIAPGNPGDSKDMKQSEPNYEGLSGNDQEKDSSQASKSSVKVPETHKAVLALRLEEKDGKIAVQTEKPESKASTDIAGQAVTINLVPVEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGHANSPKTKSSSSTPNSPVTSPALTSGHISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELISSGTGPNVPPRKICHKSAPTSPTATNVSSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKASDNATSKPTECPQAKAFSNSTERKRGSVAQKVQEFNSCLNRSQSSPQRSYSSNHSSPAKLQRTTQEPVAKTESTQEPQVVGGGSSTREKASTVLSQMVASIQPPQSPPETPQSGPKACSVEELYAVPPDAGAAKTTPKGTPVRPKSLFMSQPSAEAEAPQTTESPTTKAQKDPLTKPVTTPPSKLVTNPQSDPPAPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKDREKGKLVGLDGTVIHMLPPPPVQRHRWFTEAKGDSSEKPAIVFMYRCDPAQGQLSMDQGKARAEQSAVTEKGGAEEGSLQDSEKKKRSHSSPSQIPKKILSHVTHEVTEDFSPRDPRTVAVKQDGGGCTSVTPALHLPELEREEEKEDISDPMDLNPCSATYSNLGQSRAAMIPPKHPRQPKGALDDVIAFGGKTDQEAPNALQPTPPPLPKKMIIRANTEPLSKDLQKSLETTLCVMANPTYDIDPSWDASSAGSSVSYELKGLDAESYDSLERPLYKERRVPSAANSISSLTTLSIKDRFSNSTESLSSRRGPSGRQGRSIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAIRQSLAVHFNIQQDCGHFLAEVPKRLLPWEDPDAAEEEEDGMEATEEVKGEADGKGPDLGSEAESSHKESQAAVSRKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDAYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGPTQGLGPAEPSPTPSCPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYTRADLPRIPLRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTASPSPAHRNTLLQNWLDIKRTLLMIKFAEKSLDREGGVTLEDWLCAQYLAFATPDSLSCVVRILQPR

Target ORF information:

RefSeq Version	XM_010798541.2
Organism	Bos taurus(cattle)
Definition	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010798541.2

ORF Insert Sequence:

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEAK1 ( XM_015468517.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324003.1 Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X5, mRNA.

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CloneID	OBa10769
Clone ID Related Accession (Same CDS sequence)	XM_015468519.1 , XM_015459261.1 , XM_010817120.2 , XM_015459262.1 , XM_005222010.3 , XM_003587568.4 , XM_015468514.1 , XM_015468516.1 , XM_015468518.1 , XM_010798541.2 , XM_015468517.1 , XM_015468520.1 , XM_005222009.3 , XM_005222005.3 , XM_005222004.3 , XM_015468515.1
Accession Version	XM_015468517.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646534.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324003.1
CDS	605..5848
Translation	MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMMVADGQSVCGELSIQEHCENKPVVIGWNRNRMALNQKPLNNNNEDDVEGFSHVPKPYGNNDSAKKISNNNNGLTEVLKEIAGLDTTPQIKENETNSRETFLGRINDCYKRSLERKLPPSCMMGGMKDTQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLLSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTVPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGPGSTQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGIAPGNPGDSKDMKQSEPNYEGLSGNDQEKDSSQASKSSVKVPETHKAVLALRLEEKDGKIAVQTEKPESKASTDIAGQAVTINLVPVEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGHANSPKTKSSSSTPNSPVTSPALTSGHISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELISSGTGPNVPPRKICHKSAPTSPTATNVSSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKASDNATSKPTECPQAKAFSNSTERKRGSVAQKVQEFNSCLNRSQSSPQRSYSSNHSSPAKLQRTTQEPVAKTESTQEPQVVGGGSSTREKASTVLSQMVASIQPPQSPPETPQSGPKACSVEELYAVPPDAGAAKTTPKGTPVRPKSLFMSQPSAEAEAPQTTESPTTKAQKDPLTKPVTTPPSKLVTNPQSDPPAPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKDREKGKLVGLDGTVIHMLPPPPVQRHRWFTEAKGDSSEKPAIVFMYRCDPAQGQLSMDQGKARAEQSAVTEKGGAEEGSLQDSEKKKRSHSSPSQIPKKILSHVTHEVTEDFSPRDPRTVAVKQDGGGCTSVTPALHLPELEREEEKEDISDPMDLNPCSATYSNLGQSRAAMIPPKHPRQPKGALDDVIAFGGKTDQEAPNALQPTPPPLPKKMIIRANTEPLSKDLQKSLETTLCVMANPTYDIDPSWDASSAGSSVSYELKGLDAESYDSLERPLYKERRVPSAANSISSLTTLSIKDRFSNSTESLSSRRGPSGRQGRSIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAIRQSLAVHFNIQQDCGHFLAEVPKRLLPWEDPDAAEEEEDGMEATEEVKGEADGKGPDLGSEAESSHKESQAAVSRKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDAYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGPTQGLGPAEPSPTPSCPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYTRADLPRIPLRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTASPSPAHRNTLLQNWLDIKRTLLMIKFAEKSLDREGGVTLEDWLCAQYLAFATPDSLSCVVRILQPR

Target ORF information:

RefSeq Version	XM_015468517.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468517.1

ORF Insert Sequence:

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEAK1 ( XM_015468520.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324006.1 Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X8, mRNA.

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CloneID	OBa10769
Clone ID Related Accession (Same CDS sequence)	XM_015468519.1 , XM_015459261.1 , XM_010817120.2 , XM_015459262.1 , XM_005222010.3 , XM_003587568.4 , XM_015468514.1 , XM_015468516.1 , XM_015468518.1 , XM_010798541.2 , XM_015468517.1 , XM_015468520.1 , XM_005222009.3 , XM_005222005.3 , XM_005222004.3 , XM_015468515.1
Accession Version	XM_015468520.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646534.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324006.1
CDS	453..5696
Translation	MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMMVADGQSVCGELSIQEHCENKPVVIGWNRNRMALNQKPLNNNNEDDVEGFSHVPKPYGNNDSAKKISNNNNGLTEVLKEIAGLDTTPQIKENETNSRETFLGRINDCYKRSLERKLPPSCMMGGMKDTQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLLSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTVPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGPGSTQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGIAPGNPGDSKDMKQSEPNYEGLSGNDQEKDSSQASKSSVKVPETHKAVLALRLEEKDGKIAVQTEKPESKASTDIAGQAVTINLVPVEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGHANSPKTKSSSSTPNSPVTSPALTSGHISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELISSGTGPNVPPRKICHKSAPTSPTATNVSSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKASDNATSKPTECPQAKAFSNSTERKRGSVAQKVQEFNSCLNRSQSSPQRSYSSNHSSPAKLQRTTQEPVAKTESTQEPQVVGGGSSTREKASTVLSQMVASIQPPQSPPETPQSGPKACSVEELYAVPPDAGAAKTTPKGTPVRPKSLFMSQPSAEAEAPQTTESPTTKAQKDPLTKPVTTPPSKLVTNPQSDPPAPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKDREKGKLVGLDGTVIHMLPPPPVQRHRWFTEAKGDSSEKPAIVFMYRCDPAQGQLSMDQGKARAEQSAVTEKGGAEEGSLQDSEKKKRSHSSPSQIPKKILSHVTHEVTEDFSPRDPRTVAVKQDGGGCTSVTPALHLPELEREEEKEDISDPMDLNPCSATYSNLGQSRAAMIPPKHPRQPKGALDDVIAFGGKTDQEAPNALQPTPPPLPKKMIIRANTEPLSKDLQKSLETTLCVMANPTYDIDPSWDASSAGSSVSYELKGLDAESYDSLERPLYKERRVPSAANSISSLTTLSIKDRFSNSTESLSSRRGPSGRQGRSIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAIRQSLAVHFNIQQDCGHFLAEVPKRLLPWEDPDAAEEEEDGMEATEEVKGEADGKGPDLGSEAESSHKESQAAVSRKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDAYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGPTQGLGPAEPSPTPSCPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYTRADLPRIPLRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTASPSPAHRNTLLQNWLDIKRTLLMIKFAEKSLDREGGVTLEDWLCAQYLAFATPDSLSCVVRILQPR

Target ORF information:

RefSeq Version	XM_015468520.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468520.1

ORF Insert Sequence:

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEAK1 ( XM_005222009.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222066.1 Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X6, mRNA.

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CloneID	OBa10769
Clone ID Related Accession (Same CDS sequence)	XM_015468519.1 , XM_015459261.1 , XM_010817120.2 , XM_015459262.1 , XM_005222010.3 , XM_003587568.4 , XM_015468514.1 , XM_015468516.1 , XM_015468518.1 , XM_010798541.2 , XM_015468517.1 , XM_015468520.1 , XM_005222009.3 , XM_005222005.3 , XM_005222004.3 , XM_015468515.1
Accession Version	XM_005222009.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104535.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222009.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222066.1
CDS	657..5900
Translation	MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMMVADGQSVCGELSIQEHCENKPVVIGWNRNRMALNQKPLNNNNEDDVEGFSHVPKPYGNNDSAKKISNNNNGLTEVLKEIAGLDTTPQIKENETNSRETFLGRINDCYKRSLERKLPPSCMMGGMKDTQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLLSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTVPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGPGSTQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGIAPGNPGDSKDMKQSEPNYEGLSGNDQEKDSSQASKSSVKVPETHKAVLALRLEEKDGKIAVQTEKPESKASTDIAGQAVTINLVPVEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGHANSPKTKSSSSTPNSPVTSPALTSGHISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELISSGTGPNVPPRKICHKSAPTSPTATNVSSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKASDNATSKPTECPQAKAFSNSTERKRGSVAQKVQEFNSCLNRSQSSPQRSYSSNHSSPAKLQRTTQEPVAKTESTQEPQVVGGGSSTREKASTVLSQMVASIQPPQSPPETPQSGPKACSVEELYAVPPDAGAAKTTPKGTPVRPKSLFMSQPSAEAEAPQTTESPTTKAQKDPLTKPVTTPPSKLVTNPQSDPPAPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKDREKGKLVGLDGTVIHMLPPPPVQRHRWFTEAKGDSSEKPAIVFMYRCDPAQGQLSMDQGKARAEQSAVTEKGGAEEGSLQDSEKKKRSHSSPSQIPKKILSHVTHEVTEDFSPRDPRTVAVKQDGGGCTSVTPALHLPELEREEEKEDISDPMDLNPCSATYSNLGQSRAAMIPPKHPRQPKGALDDVIAFGGKTDQEAPNALQPTPPPLPKKMIIRANTEPLSKDLQKSLETTLCVMANPTYDIDPSWDASSAGSSVSYELKGLDAESYDSLERPLYKERRVPSAANSISSLTTLSIKDRFSNSTESLSSRRGPSGRQGRSIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAIRQSLAVHFNIQQDCGHFLAEVPKRLLPWEDPDAAEEEEDGMEATEEVKGEADGKGPDLGSEAESSHKESQAAVSRKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDAYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGPTQGLGPAEPSPTPSCPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYTRADLPRIPLRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTASPSPAHRNTLLQNWLDIKRTLLMIKFAEKSLDREGGVTLEDWLCAQYLAFATPDSLSCVVRILQPR

Target ORF information:

RefSeq Version	XM_005222009.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222009.3

ORF Insert Sequence:

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEAK1 ( XM_005222005.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222062.1 Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa10769
Clone ID Related Accession (Same CDS sequence)	XM_015468519.1 , XM_015459261.1 , XM_010817120.2 , XM_015459262.1 , XM_005222010.3 , XM_003587568.4 , XM_015468514.1 , XM_015468516.1 , XM_015468518.1 , XM_010798541.2 , XM_015468517.1 , XM_015468520.1 , XM_005222009.3 , XM_005222005.3 , XM_005222004.3 , XM_015468515.1
Accession Version	XM_005222005.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104535.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222005.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222062.1
CDS	638..5881
Translation	MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMMVADGQSVCGELSIQEHCENKPVVIGWNRNRMALNQKPLNNNNEDDVEGFSHVPKPYGNNDSAKKISNNNNGLTEVLKEIAGLDTTPQIKENETNSRETFLGRINDCYKRSLERKLPPSCMMGGMKDTQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLLSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTVPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGPGSTQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGIAPGNPGDSKDMKQSEPNYEGLSGNDQEKDSSQASKSSVKVPETHKAVLALRLEEKDGKIAVQTEKPESKASTDIAGQAVTINLVPVEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGHANSPKTKSSSSTPNSPVTSPALTSGHISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELISSGTGPNVPPRKICHKSAPTSPTATNVSSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKASDNATSKPTECPQAKAFSNSTERKRGSVAQKVQEFNSCLNRSQSSPQRSYSSNHSSPAKLQRTTQEPVAKTESTQEPQVVGGGSSTREKASTVLSQMVASIQPPQSPPETPQSGPKACSVEELYAVPPDAGAAKTTPKGTPVRPKSLFMSQPSAEAEAPQTTESPTTKAQKDPLTKPVTTPPSKLVTNPQSDPPAPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKDREKGKLVGLDGTVIHMLPPPPVQRHRWFTEAKGDSSEKPAIVFMYRCDPAQGQLSMDQGKARAEQSAVTEKGGAEEGSLQDSEKKKRSHSSPSQIPKKILSHVTHEVTEDFSPRDPRTVAVKQDGGGCTSVTPALHLPELEREEEKEDISDPMDLNPCSATYSNLGQSRAAMIPPKHPRQPKGALDDVIAFGGKTDQEAPNALQPTPPPLPKKMIIRANTEPLSKDLQKSLETTLCVMANPTYDIDPSWDASSAGSSVSYELKGLDAESYDSLERPLYKERRVPSAANSISSLTTLSIKDRFSNSTESLSSRRGPSGRQGRSIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAIRQSLAVHFNIQQDCGHFLAEVPKRLLPWEDPDAAEEEEDGMEATEEVKGEADGKGPDLGSEAESSHKESQAAVSRKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDAYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGPTQGLGPAEPSPTPSCPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYTRADLPRIPLRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTASPSPAHRNTLLQNWLDIKRTLLMIKFAEKSLDREGGVTLEDWLCAQYLAFATPDSLSCVVRILQPR

Target ORF information:

RefSeq Version	XM_005222005.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222005.3

ORF Insert Sequence:

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEAK1 ( XM_005222004.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222061.1 Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X1, mRNA.

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CloneID	OBa10769
Clone ID Related Accession (Same CDS sequence)	XM_015468519.1 , XM_015459261.1 , XM_010817120.2 , XM_015459262.1 , XM_005222010.3 , XM_003587568.4 , XM_015468514.1 , XM_015468516.1 , XM_015468518.1 , XM_010798541.2 , XM_015468517.1 , XM_015468520.1 , XM_005222009.3 , XM_005222005.3 , XM_005222004.3 , XM_015468515.1
Accession Version	XM_005222004.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104535.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222004.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222061.1
CDS	517..5760
Translation	MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMMVADGQSVCGELSIQEHCENKPVVIGWNRNRMALNQKPLNNNNEDDVEGFSHVPKPYGNNDSAKKISNNNNGLTEVLKEIAGLDTTPQIKENETNSRETFLGRINDCYKRSLERKLPPSCMMGGMKDTQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLLSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTVPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGPGSTQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGIAPGNPGDSKDMKQSEPNYEGLSGNDQEKDSSQASKSSVKVPETHKAVLALRLEEKDGKIAVQTEKPESKASTDIAGQAVTINLVPVEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGHANSPKTKSSSSTPNSPVTSPALTSGHISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELISSGTGPNVPPRKICHKSAPTSPTATNVSSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKASDNATSKPTECPQAKAFSNSTERKRGSVAQKVQEFNSCLNRSQSSPQRSYSSNHSSPAKLQRTTQEPVAKTESTQEPQVVGGGSSTREKASTVLSQMVASIQPPQSPPETPQSGPKACSVEELYAVPPDAGAAKTTPKGTPVRPKSLFMSQPSAEAEAPQTTESPTTKAQKDPLTKPVTTPPSKLVTNPQSDPPAPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKDREKGKLVGLDGTVIHMLPPPPVQRHRWFTEAKGDSSEKPAIVFMYRCDPAQGQLSMDQGKARAEQSAVTEKGGAEEGSLQDSEKKKRSHSSPSQIPKKILSHVTHEVTEDFSPRDPRTVAVKQDGGGCTSVTPALHLPELEREEEKEDISDPMDLNPCSATYSNLGQSRAAMIPPKHPRQPKGALDDVIAFGGKTDQEAPNALQPTPPPLPKKMIIRANTEPLSKDLQKSLETTLCVMANPTYDIDPSWDASSAGSSVSYELKGLDAESYDSLERPLYKERRVPSAANSISSLTTLSIKDRFSNSTESLSSRRGPSGRQGRSIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAIRQSLAVHFNIQQDCGHFLAEVPKRLLPWEDPDAAEEEEDGMEATEEVKGEADGKGPDLGSEAESSHKESQAAVSRKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDAYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGPTQGLGPAEPSPTPSCPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYTRADLPRIPLRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTASPSPAHRNTLLQNWLDIKRTLLMIKFAEKSLDREGGVTLEDWLCAQYLAFATPDSLSCVVRILQPR

Target ORF information:

RefSeq Version	XM_005222004.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222004.3

ORF Insert Sequence:

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEAK1 ( XM_015468515.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324001.1 Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X3, mRNA.

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CloneID	OBa10769
Clone ID Related Accession (Same CDS sequence)	XM_015468519.1 , XM_015459261.1 , XM_010817120.2 , XM_015459262.1 , XM_005222010.3 , XM_003587568.4 , XM_015468514.1 , XM_015468516.1 , XM_015468518.1 , XM_010798541.2 , XM_015468517.1 , XM_015468520.1 , XM_005222009.3 , XM_005222005.3 , XM_005222004.3 , XM_015468515.1
Accession Version	XM_015468515.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pseudopodium-enriched atypical kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646534.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324001.1
CDS	596..5839
Translation	MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNKTNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMMVADGQSVCGELSIQEHCENKPVVIGWNRNRMALNQKPLNNNNEDDVEGFSHVPKPYGNNDSAKKISNNNNGLTEVLKEIAGLDTTPQIKENETNSRETFLGRINDCYKRSLERKLPPSCMMGGMKDTQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDVLLSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTVPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGPGSTQSMVSSDSTSPDSSLTEESRSETASSLSQKICNGGIAPGNPGDSKDMKQSEPNYEGLSGNDQEKDSSQASKSSVKVPETHKAVLALRLEEKDGKIAVQTEKPESKASTDIAGQAVTINLVPVEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGHANSPKTKSSSSTPNSPVTSPALTSGHISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELISSGTGPNVPPRKICHKSAPTSPTATNVSSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKASDNATSKPTECPQAKAFSNSTERKRGSVAQKVQEFNSCLNRSQSSPQRSYSSNHSSPAKLQRTTQEPVAKTESTQEPQVVGGGSSTREKASTVLSQMVASIQPPQSPPETPQSGPKACSVEELYAVPPDAGAAKTTPKGTPVRPKSLFMSQPSAEAEAPQTTESPTTKAQKDPLTKPVTTPPSKLVTNPQSDPPAPFPPPRSTSSPYHASNLLQRHFTNWTKPTSPTRSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKDREKGKLVGLDGTVIHMLPPPPVQRHRWFTEAKGDSSEKPAIVFMYRCDPAQGQLSMDQGKARAEQSAVTEKGGAEEGSLQDSEKKKRSHSSPSQIPKKILSHVTHEVTEDFSPRDPRTVAVKQDGGGCTSVTPALHLPELEREEEKEDISDPMDLNPCSATYSNLGQSRAAMIPPKHPRQPKGALDDVIAFGGKTDQEAPNALQPTPPPLPKKMIIRANTEPLSKDLQKSLETTLCVMANPTYDIDPSWDASSAGSSVSYELKGLDAESYDSLERPLYKERRVPSAANSISSLTTLSIKDRFSNSTESLSSRRGPSGRQGRSIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFGVDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAIRQSLAVHFNIQQDCGHFLAEVPKRLLPWEDPDAAEEEEDGMEATEEVKGEADGKGPDLGSEAESSHKESQAAVSRKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDAYERQVCLLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGPTQGLGPAEPSPTPSCPTRLIVSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEKEYTRADLPRIPLRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTASPSPAHRNTLLQNWLDIKRTLLMIKFAEKSLDREGGVTLEDWLCAQYLAFATPDSLSCVVRILQPR

Target ORF information:

RefSeq Version	XM_015468515.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468515.1

ORF Insert Sequence:

ATGTCTGCTT GTAATACCTT TACTGAACAC GTTTGGAAAC CTGGTGAATG CAAGAACTGC 
TTTAAACCTA AAAGTTTACA CCAGCTACCC CCAGACCCTG AGAAGGCACC CATCACCCAT 
GGCAACAAAA CTAATGCCAA CCATAGTAAC AACCACCGTA TCAGGAACAC TGGAAACTTC 
CGGCCTCCTG TGGCTAAAAA ACCCACTATC GCTGTGAAGC CCACTATGAT GGTGGCAGAT 
GGGCAAAGTG TGTGTGGTGA GCTTAGTATA CAAGAACACT GTGAGAATAA ACCTGTCGTC 
ATAGGATGGA ACCGAAACAG GATGGCCCTG AATCAGAAAC CACTTAATAA TAATAATGAA 
GATGATGTTG AAGGATTTAG CCATGTCCCT AAGCCTTATG GGAATAACGA TAGTGCAAAG 
AAGATTTCAA ATAACAATAA TGGACTAACT GAGGTGTTAA AGGAGATAGC AGGCTTGGAT 
ACCACCCCTC AAATAAAAGA AAATGAGACT AACTCCAGAG AGACATTCTT AGGAAGAATA 
AATGATTGCT ATAAACGATC CCTGGAAAGA AAGCTTCCAC CAAGTTGCAT GATGGGCGGG 
ATGAAGGATA CTCAGGGCAA GCACGTTATT CTGAGTGGGA GCACAGAAGT GATCAGTAAT 
GAAGGGGGTC GATTCTGTTA CCCAGAGTTT TCTAGTGGTG AAGAGAGTGA GGAAGATGTG 
CTTTTGAGTA ACATGGAGGA GGAGCATGAG AGCTGGGATG AGAGTGATGA AGAACTGCTG 
GCCATGGAGA TTCGCATGAG AGGGCAGCCT CGCTTTGCCA ACTTTCGGGC AAACACTTTG 
TCTCCTGTGC GATTCTTTGT GGACAAAAAA TGGAATACCG TCCCCCTGCG AAATAAATCT 
CTGCAAAGAA TCTGTGCTGT AGACTATGAT GACAGTTATG ATGAAATCCT GAATGGTTAT 
GAAGAGAATT CTGTGGTCTC CTACGGACCA GGAAGCACTC AGAGCATGGT GTCATCCGAC 
TCCACATCCC CAGATTCCTC TTTAACAGAA GAGTCACGTT CTGAGACAGC CAGCAGTTTA 
TCCCAGAAGA TCTGTAATGG GGGAATTGCT CCTGGTAACC CGGGAGATTC TAAGGACATG 
AAGCAAAGTG AGCCCAATTA TGAAGGCCTC TCTGGTAATG ATCAGGAGAA GGACTCCTCA 
CAAGCCTCTA AAAGCTCGGT AAAAGTTCCA GAGACACACA AAGCAGTCCT TGCTCTCCGA 
TTAGAAGAGA AGGATGGCAA GATTGCTGTA CAGACTGAGA AGCCAGAAAG TAAAGCCTCT 
ACAGATATTG CTGGGCAAGC GGTAACCATA AACCTTGTTC CTGTGGAAGA GCAAGCAAAG 
CCCTACCGAG TTGTGAATCT GGAACAGCCT TTGTGCAAGC CGTATACTGT CGTGGATGTG 
TCAGCAGCCA TGGCCAGTGA GCACCTTGAG GGCCATGCTA ACAGTCCCAA GACTAAAAGT 
TCATCTTCTA CTCCAAACTC TCCGGTGACA TCACCTGCAT TGACATCAGG ACACATAAGT 
GCCCATTTCC AAAAATCCAG TGCCATCCGC TACCAGGAAG TATGGACTTC CAGCACCAGT 
CCACGACAGA AGATACCTAA GGTTGAATTA ATCAGCAGTG GGACTGGACC AAATGTCCCT 
CCAAGGAAAA TCTGTCACAA ATCAGCACCT ACTTCACCCA CAGCTACAAA TGTTTCCTCC 
AAAACCATCC CTGTGAAGTC ACCCAATTTG TCTGAAATAA AATTTAACAG TTATAACAAT 
GCCGGTATGC CACCTTTTCC AATTATCATT CATGATGAGC CAACTTATGC TCGGAGTTCC 
AAAAATGCTA TCAAAGTTCC CATTGTAATC AATCCAAATG CGTATGACAA TCTAGCCATT 
TACAAGAGTT TTCTCGGAAC CAGTGGAGAA CTTTCAGTGA AGGAGAAAAC CACAAGTGTA 
ATAAGCCACA CTTACGAAGA AATAGAGACT GAAAGCAAAG CGTCTGATAA CGCTACTAGC 
AAACCCACTG AATGTCCCCA AGCTAAAGCG TTTTCAAACA GCACAGAGCG TAAAAGGGGC 
TCAGTGGCTC AGAAGGTTCA GGAGTTTAAC AGCTGTCTTA ACAGAAGTCA GTCTTCACCA 
CAGAGAAGCT ACAGTTCCAA CCACAGCTCC CCAGCAAAGC TCCAGAGAAC CACTCAAGAA 
CCTGTGGCCA AAACAGAAAG CACTCAGGAG CCTCAGGTGG TGGGCGGTGG CAGTAGCACC 
CGAGAGAAAG CGAGCACCGT GCTTTCTCAG ATGGTGGCTT CTATCCAGCC CCCACAGTCT 
CCTCCAGAAA CGCCCCAGTC TGGCCCTAAA GCTTGCAGTG TGGAAGAGCT TTATGCAGTT 
CCTCCCGATG CCGGTGCTGC CAAGACTACA CCCAAGGGTA CACCGGTGCG CCCCAAATCT 
CTCTTCATGT CCCAGCCCAG CGCTGAGGCT GAAGCACCTC AGACCACAGA AAGTCCTACC 
ACCAAAGCAC AGAAAGATCC ACTCACAAAG CCAGTCACCA CCCCTCCCTC CAAATTAGTG 
ACGAACCCCC AAAGCGATCC ACCAGCCCCT TTTCCCCCAC CACGCTCGAC TTCGTCCCCT 
TACCATGCGA GTAACCTTTT GCAGAGGCAT TTCACCAACT GGACCAAGCC AACCAGCCCG 
ACCAGGTCAA CGGAAGCTGA ATCAGTTTTG CACTCTGAAG GCAGCAGGCG GGCAGCTGAC 
GCAAAACCTA AACGCTGGAT CTCATTTAAA AGTTTCTTCC GCCGTCGGAA AACAGACGAG 
GAGGATGACA AAGAGAAAGA CCGGGAGAAA GGGAAACTGG TGGGCCTGGA TGGCACAGTC 
ATCCACATGC TGCCACCTCC TCCAGTTCAG CGCCATCGCT GGTTCACAGA GGCAAAAGGA 
GACTCCAGCG AGAAGCCAGC CATTGTCTTC ATGTACAGGT GCGACCCTGC CCAAGGCCAG 
CTCAGCATGG ATCAGGGCAA AGCCAGGGCA GAGCAGTCAG CAGTCACAGA GAAGGGGGGA 
GCAGAGGAGG GGTCACTACA GGACTCAGAG AAGAAGAAAA GGAGTCATTC ATCTCCATCA 
CAGATTCCTA AGAAAATTCT CAGTCACGTG ACCCACGAAG TGACAGAGGA TTTTTCTCCT 
CGGGATCCAA GAACTGTTGC TGTGAAGCAA GATGGCGGGG GCTGCACTTC AGTCACACCA 
GCATTGCACC TACCTGAACT GGAAAGAGAA GAGGAAAAAG AAGACATTTC AGATCCTATG 
GACCTGAATC CCTGTAGTGC AACATACAGC AATTTAGGAC AATCTAGAGC AGCCATGATA 
CCTCCCAAGC ATCCACGGCA GCCTAAGGGA GCTTTGGATG ATGTCATCGC CTTTGGCGGG 
AAAACAGACC AAGAAGCACC CAATGCTTTG CAACCAACAC CACCCCCACT GCCAAAGAAA 
ATGATCATAA GAGCCAATAC AGAGCCACTC TCCAAAGACC TCCAGAAGTC CCTGGAAACT 
ACCCTTTGTG TCATGGCTAA TCCCACCTAC GATATCGACC CGAGCTGGGA TGCCAGCAGT 
GCTGGCTCCT CGGTCAGCTA TGAACTCAAG GGACTGGACG CTGAGTCTTA TGACTCCTTG 
GAGAGACCTT TGTACAAGGA GAGACGTGTG CCCTCAGCAG CAAACAGCAT CTCCAGCCTA 
ACCACTCTCA GTATTAAAGA TAGGTTTTCC AACAGCACAG AGTCCCTGTC CAGCCGGCGG 
GGTCCCTCTG GTAGACAGGG CCGGAGTATC CAGAAGCCAC AGAGACAAGC ACTTTATCGA 
GGACTTGAGA ATCGTGAAGA AGTGGTGGGT AAAATCCGAA GCCTTCACAC AGATGCGTTG 
AAGAAACTGG CTGTTAAATG TGAAGACCTC TTCATGGCTG GGCAGAAAGA CCAACTCCGT 
TTTGGGGTAG ACAGCTGGTC AGACTTCAGG CTGACCAGTG ACAAGCCATG CTGTGAGGCC 
GGTGATGCAG TTTACTATAC AGCTTCATAC GCAAAAGATC CACTTAATAA CTATGCGGTC 
AAGATCTGTA AGAGCAAAGC TAAAGAATCG CAGCAATATT ATCACAGCTT GGCTATCCGG 
CAGAGTCTGG CTGTCCACTT TAACATCCAG CAGGACTGTG GTCACTTCCT TGCTGAAGTT 
CCTAAGCGCC TGCTTCCCTG GGAGGATCCC GACGCTGCCG AAGAGGAAGA GGATGGCATG 
GAAGCAACGG AAGAGGTGAA AGGAGAAGCT GATGGGAAGG GCCCAGACCT GGGCTCCGAA 
GCAGAGTCGT CCCACAAAGA AAGCCAGGCA GCCGTCAGCA GGAAGCAGAG AAGCCACGTT 
GTGGTCATCA CCAGAGAGGT TCCCTGTCTC ACAGTGGCCG ACTTCGTGCG AGACTCCCTG 
GCACAGCATG GGAAAAGCCC GGATGCATAC GAGAGGCAAG TGTGTCTGCT GCTTCTGCAG 
CTGTGCTCGG GCCTGGAGCA CCTCAAACCC TACCACGTCA CCCACTGCGA TCTGCGTCTG 
GAGAACCTGC TGCTCGTCCA CTACCAGCCG GGGGGGCCCA CTCAAGGCCT GGGGCCTGCA 
GAACCCAGCC CCACCCCGTC CTGTCCCACG AGGCTCATCG TCAGCAACTT CTCCCAGGCC 
AAGCAGAAGA GCCATTTGGT AGATCCTGAG ATCCTCCGGG ATCAGTCCCG ACTCGCCCCA 
GAGATCATCA CAGCCACACA ATATAAAAAG TGCGATGAGT TCCAGACAGG CATCCTCATC 
TACGAGATGC TGCACCTGCC CAACCCTTTT GATGAGAACC CAGAGCTGAA GGAGAAGGAG 
TACACTCGGG CTGACCTGCC CCGCATCCCG CTCCGCTCCC CCTATTCCCG GGGCCTGCAG 
CAGCTGGCCA GCTGCCTCCT AAATCCCAAC CCTTCTGAGC GGATCCTCAT TTCAGATGCC 
AAAGGCATCC TCCAGTGTCT GCTCTGGGGC CCCCGGGAAG ATCTCTTCCA GACTTTCACC 
GCCTCCCCCA GCCCCGCTCA CAGGAACACC CTGCTCCAGA ACTGGCTGGA CATCAAGCGC 
ACACTGCTCA TGATCAAGTT TGCCGAGAAG TCCCTGGACC GGGAGGGTGG CGTCACCCTG 
GAGGACTGGC TTTGTGCTCA GTATCTGGCT TTTGCCACTC CAGACTCTCT CAGCTGTGTG 
GTGAGAATCC TGCAGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PEAK1
Entrez Gene ID	786652
Full Name	pseudopodium enriched atypical kinase 1
General protein information	Preferred Names pseudopodium enriched atypical kinase 1 Names pseudopodium-enriched atypical kinase 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Bos taurus (cattle)	LOC786652	XP_005222067.1
Pan troglodytes (chimpanzee)	PEAK1	XP_510688.2
Danio rerio (zebrafish)	LOC565111	XP_001921602.2
Xenopus tropicalis (tropical clawed frog)	LOC100496576	XP_004916046.1
Homo sapiens (human)	PEAK1	NP_079052.2
	LOC708528	XP_001106139.2
Canis lupus familiaris (dog)	PEAK1	XP_005638710.1
Mus musculus (house mouse)	Peak1	NP_766512.2
Rattus norvegicus (Norway rat)	Peak1	NP_001101619.1
Gallus gallus (chicken)	PEAK1	NP_001244188.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PEAK1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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