TJP1 cDNA ORF clone, Bos taurus(cattle)

The following TJP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TJP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa228431 XM_024982002.1
Latest version!
Bos taurus tight junction protein 1 (TJP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa228432 XM_024982003.1
Latest version!
Bos taurus tight junction protein 1 (TJP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa228433 XM_024982004.1
Latest version!
Bos taurus tight junction protein 1 (TJP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa228434 XM_024982005.1
Latest version!
Bos taurus tight junction protein 1 (TJP1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa228435 XM_024982006.1
Latest version!
Bos taurus tight junction protein 1 (TJP1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa228436 XM_024982007.1
Latest version!
Bos taurus tight junction protein 1 (TJP1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa228437 XM_024982008.1
Latest version!
Bos taurus tight junction protein 1 (TJP1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa228438 XM_024982009.1
Latest version!
Bos taurus tight junction protein 1 (TJP1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa228439 XM_024982010.1
Latest version!
Bos taurus tight junction protein 1 (TJP1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa228440 XM_024982011.1
Latest version!
Bos taurus tight junction protein 1 (TJP1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa228441 XM_024982012.1
Latest version!
Bos taurus tight junction protein 1 (TJP1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa45684 XM_015468497.1
Latest version!
Bos taurus tight junction protein 1 (TJP1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa45684 XM_010817146.1
Latest version!
Bos taurus tight junction protein 1 (TJP1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa228431
    Clone ID Related Accession (Same CDS sequence) XM_024982002.1
    Accession Version XM_024982002.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5568bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGGGCCC TGGCCGCGAG 
    CGGAGCGGGG ACAAGATGAA GTACCAGAAG TACCTGACGG TGCTGCAGAT GGCCATCGGC
    GTCACGCCGT CCAACCGCGG CAGCCTGCTG CCGCTCAAGA GGAGGCTGTG GGTAACGCCG
    TCCTCTGAAA ACCCCAGTGG TGCTACTTCT AGTGTCAGCC AAGGAAAGCC TTCTTTAAGA
    CGAATTAAAG GGAGACTGCA CAGGAGCAAG AGCCTCGATA GCATCGATTT CTGTGAACTC
    ACTAACACAA CAATGGAGGA AACAGCTATA TGGGAACAAC ACACGGTGAC ACTTCACAGG
    GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC ACATTTCCAG
    AGCGGGGAAA CCTCCATCGT GATTTCAGAT GTGCTGAAGG GGGGGCCTGC GGAAGGACAG
    CTGCAGGAAA ATGACCGGGT GGCAATGGTT AATGGAGTCT CCATGGATAA TGTTGAGCAT
    GCGTTTGCTG TTCAGCAACT AAGGAAAAGT GGGAAAAATG CAAAAATAAC CATTCGAAGG
    AAGAAGAAAG TTCAGATCCC AGTAAATCGT CCTGATCCTG AGCCTGTGTC TGAGAACGAA
    GAGGACAGTT ACGACGAGGA AGCGCATGAC CCGAGAAGTG GCCGTGGGGC CCTGGCAAAC
    AGGAGGAGCG AGAAGAGCTG GGGCCGAGAT AGGAGTGCCA GTCGGGAGAG GAGTCTGTCC
    CCGCGGTCTG ACAGGCGGTC TGTAGCCTCC AGTCAGCCTG CTAGACCCAC CAAAGTCACC
    CTGGTGAAGT CCCGGAAGAA TGAAGAATAT GGTCTTCGAT TGGCCAGCCA TATATTTGTG
    AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT
    GTATTGAAGA TAAATGGTAC CGTGACGGAA AACATGTCAC TGACAGATGC GAAGACGCTG
    ATCGAGAGGT CTAAGGGCAA GTTAAAGATG GTGGTTCAGA GAGATGAGCG GGCTACCCTG
    CTGAATGTCC CTGACCTTTC TGACAGCATC CACTCTGCTG ACGCCTCCGA GAGGGACGAT
    ATTTCAGAAA TCCAGTCACT GGCATCAGAC CATTCAGGTC GATCCCACGA CCGGCCTCCC
    CGTCACAGCC GCTCTCGGTC TCCTGACCAG CGGTCCGAGC CTTCTGACCA TTCTAGACAC
    TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA
    CCAGGGGCTG TCTCGACTCC GGTAAAGCAT GCAGATGATC ATACACCTAA AACAGTGGAA
    GAAGTTGTAG TTGAGCGAAA TGAGAAACAA GCACCCAATC TTCCAGAACC AAAGCCTGTG
    TACGCACAGG TAGGACAGCC AGACGTGGAT TTACCTGTCA GTCCATCTGA TGGCGTCCTG
    CCCAACTCAA CTCATGAAGA TGGCATTCTT CGGCCCAGCA TGAAGTTAGT AAAATTCAGG
    AAAGGAGATA GTGTGGGTCT TCGACTAGCT GGTGGAAACG ATGTTGGCAT ATTTGTAGCT
    GGTGTTCTCG AAGATAGCCC TGCTGCCAAA GAAGGCTTGG AGGAGGGCGA CCAGATCCTC
    AGGGTAAACA ATGTAGATTT CACAAATATC ATAAGAGAAG AAGCCGTCCT CTTCCTGCTT
    GACCTCCCAA AAGGAGAAGA AGTGACCATA TTGGCTCAGA AGAAGAAAGA TGTTTATCGT
    CGCATCGTAG AATCCGATGT GGGTGATTCT TTCTATATCA GAACCCATTT TGAATATGAG
    AAGGAATCTC CCTATGGACT CAGTTTTAAC AAAGGAGAGG TGTTCCGTGT CGTGGACACT
    CTGTACAATG GGAAGCTGGG GTCCTGGCTT GCCATTCGGA TTGGCAAAAA CCATAAAGAG
    GTGGAACGTG GCATCATCCC AAACAAAAAC AGAGCTGAGC AGTTAGCCAG TGTGCAGTAT
    ACACTTCCCA AGACAGCAGG GGGAGACCGC GCCGACTTCT GGAGGTTCCG CGGTCTGCGC
    AGCTCCAAGA GAAACCTGCG CAAGAGCCGA GAAGACCTCT CAGCCCAGCC AGTCCAGACC
    AAGTTCCCGG CGTATGAGAG GGTGGTTCTT CGAGAAGCTG GATTCCTGAG GCCTGTAACC
    ATCTTTGGAC CAATAGCTGA TGTTGCCAGG GAGAAACTGG CAAGAGAGGA GCCAGATATT
    TACCAGATCG CAAAAAGTGA ACCTCGGGAT GCAGGAACCG ACCAGCGCAG CTCTGGCATT
    ATTCGCCTCC ACACAATAAA GCAGATAATA GATCAGGACA AACATGCCTT ACTGGATGTA
    ACGCCCAATG CAGTAGACCG TCTGAACTAT GCCCAGTGGT ATCCAATCGT GGTGTTCCTT
    AACCCGGACT CAAAGCAGGG TGTGAAAACA ATGCGAATGA GGTTATGTCC AGAATCTCGG
    AAAAGCGCCA GGAAGTTATA TGAGCGCTCT CATAAACTTC GTAAAAATAA TCACCATCTT
    TTTACAACTA CAATTAACTT AAATTCAATG AATGATGGCT GGTATGGTGC ACTGAAAGAA
    GCAATCCAAC AACAACAGAA CCAGTTGGTA TGGGTTTCTG AGGGAAAGGC GGATGGTGCT
    ACAAGTGATG ACCTTGATTT GCATGATGAT CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT
    GAATACTCAA TGTATAGCAC GGACAGTAGA CACACTTCTG ACTATGAAGA CACAGACACA
    GAAGGCGGGG CCTACACTGA TCAAGAACTA GATGAGACCC TAAATGATGA GGTGGGGACT
    CCCCCGGAGT CTGCCATTAC CCGGTCCTCT GAGCCTGTCA GAGAGGACTC CTCTGGAATG
    CATCCTGACC ACCAGGCATA TCCTCCCTAC TCACCACAAG CGCAGCCACA GCCAGGCCAT
    AGAATAGACT CCCCTGGATT TAAAACAGCC TCTCAACAGA AAGCAGAAGC CTCATCTCCA
    GGCCCTTACC TTTCGCCTGA AACAAACCCG GCACCATCAG CCTCTGCTGT TAATCCTCAC
    GTAGACTTAA CTAATGTCAG ACTGGAGGAG CCGAGCCCAG CTCCCCCCTC CTCTGACTCA
    CCACACGCCG ACTCTTTACG AGCTCCAGGC ACTGAGGCCG CTCACGTGCA GCTCAAAGGT
    CCAGAGCCGT CCCTGTCGTC GCATGTGGGC CCAGCAAAGG TGTACAGGAA GGACCCCTAC
    CCTGAGGAGA TGATGAGACA GAACCACGTC TTGAAGCAGC CGGCTGTGGT TCACCCTGGG
    CAGAGGCCAG ACAAGGAGCA AGGCCTGAGC TACGAGCCGC AGCCTCCGTA CACAGAGCGA
    CAGGCTGGCA GGGAGTTGGA GCGGCCTGCA TACAGATACG ATGCCTCTAG CTACCCGGAG
    CAGTTTGCTC GGAACTATGA CCATCGTCTG CGCTATGACG ACCGCCTCCT CACCTATGAG
    GAACAATGGC CCTACTACGA TGACAAGCAG CCTTACCAGC CCCGGCCTCT TGATGGTCAG
    CCTCCCGCGG AGCCAACAGA ACGAGGGTAT TTCCCCCGTT TTGAGGAGCC AGTGCCCCTG
    TCCTATGAAG GCCGAGCGCA CTACGACCCA CTGCCCAGGA CCTCCACCTT GCGGCACGAA
    GAGCCTCCCG CCCCTGCCCC TGGGTACGAC GTGCACGGAA GGTACCGGCC TGACGTGCAG
    CCCTACTCCA CTGCTGGCCC CAAGGTGCCC CCTGAGCCCA AGCAGTACTT GGACCAGTAC
    CCTCGGAGTT ACGAGCCAGC GCCAGCCCAG GGCTTCCCCT CCAGGGCAGG CCCAGGCCAC
    TACGAGCCTC TTCACAGCAC CACGCTCGCT CCACCACTTG GCCCCTCGTC CCAGCACAAG
    CCCGAAGTCC CGCCCACAGG CACCAAACCC CTGCCTCCAC CCCCAGCTCC GGCCGAGGAG
    GAGGAAGACC CAGCCATGAA GCCACAATCC GTGCTCACCA GAGTGAAGAT GTTTGAGAAT
    AAGAGGTCTG CTTCCATGGA GAACAGGAAG GATGAGAGCC ATTCCACTAG TTTCAAGCCC
    CCAGAAGTCA CGTCAAAACC GGCAGGTGCT CCTGTCCCAG GTCCCAAAGC CCCTGCTCCG
    AGTCAGCTCG GTGAACACGA CAGAGCAGCA CATAGGATCC CTGAACCACA GAGGCCTCAC
    ATGAAGCCAC CTGAAGACAT TGTTCGGGCA AATCACTATG ACCCCGAGGA GGATGAGGAG
    TACTACCGGA AGCAGCTCTC CTACTTCGAT CGGAGAAGCT TTGAGGCCAA GCCCCCCACC
    CACATCCCCA CTAGCCACCT CTCCGAGCCC ACCAGGCCGG CTCACCCCCA ATCTCAGCCA
    CAGTTTGCCG GTTACTCTTC CAAGGGGAAG GCTGCTGAGG CCGAAGCTGT GGACAGGTCA
    TTTGGGGAGA AGCGCTATGA CCCGCTGCAG GCGACGCCCC CCCCTCCGCC GCTGCCTGTT
    CAGTACAGCC AGGGTGCTCA ACCCAGCCCC AACACGGCTC TTGGCCTCCA CGCACACACC
    AAGGGGGCGC ATGGTGAAGG TAATGCCATG TCGTTGGATT TTCAGAACTC GTTAGTGTCC
    AAACCGGACC CACCTCCATC TCAGAATAAG CCAGCAGCTT ACAGACCAGC CCCTCGAGAG
    GATGCTGTCC AGTCTGGCTT CTACCCACAA AAGAGTTTCC CAGAGAAAGC CCCAGCTAAT
    GGGGTCGAGC AGACGCAGAA AACCGTCACG CCAGCATACA ATCGATTCAC ACCAAAACCA
    TACACAAGCT CTGCACGGCC ATTTGAACGC AAGTTTGAAA GCCCTAAATT CAACCACAAT
    CTCTTGCCGA ATGAAACCGC ACACAAACCT GACCTGTCCT CCAAAGCCCC CGCTTCTCCG
    AAAACCCTCC TGAAGTCTGC ACAGCCTCCT GAGTTTGACA GTGGCGTGGA GACCCTCTCT
    GTCCACACTG ACAACAAGCT GAAATACCAA GTAAACAGTG TTAGCATGGG GCCGAGAGCA
    GTGCCCGTGA GTCCCTCAGC CGTGGAGGAA GATGAAGACG AAGACGGTCA CACAGTGGTG
    GCTACTGCCC GCGGCGTGTT TAACAGCAAT GGTGGGGTGC TGAGCTCCAT TGAGACCGGT
    GTCAGCATCA TCATCCCCCA GGGAGCCATT CCCGAGGGGG TGGAACAGGA GATCTACTTC
    AAGGTCTGCC GCGACAACAG CATCCTGCCG CCTTTGGACA AAGAGAAGGG TGAGACCCTG
    CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG
    CGCCTACCAC ACTGTGCGTC CATGACTCCT GACGGTTGGT CTTTTGCTCT AAAATCATCC
    GACTCCTCGT CGGGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCAAAT
    TATCTTGTTG GAGCAAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837770.1
    CDS50..5617
    Translation

    Target ORF information:

    RefSeq Version XM_024982002.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 1 (TJP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982002.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGGGCCC TGGCCGCGAG 
    CGGAGCGGGG ACAAGATGAA GTACCAGAAG TACCTGACGG TGCTGCAGAT GGCCATCGGC
    GTCACGCCGT CCAACCGCGG CAGCCTGCTG CCGCTCAAGA GGAGGCTGTG GGTAACGCCG
    TCCTCTGAAA ACCCCAGTGG TGCTACTTCT AGTGTCAGCC AAGGAAAGCC TTCTTTAAGA
    CGAATTAAAG GGAGACTGCA CAGGAGCAAG AGCCTCGATA GCATCGATTT CTGTGAACTC
    ACTAACACAA CAATGGAGGA AACAGCTATA TGGGAACAAC ACACGGTGAC ACTTCACAGG
    GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC ACATTTCCAG
    AGCGGGGAAA CCTCCATCGT GATTTCAGAT GTGCTGAAGG GGGGGCCTGC GGAAGGACAG
    CTGCAGGAAA ATGACCGGGT GGCAATGGTT AATGGAGTCT CCATGGATAA TGTTGAGCAT
    GCGTTTGCTG TTCAGCAACT AAGGAAAAGT GGGAAAAATG CAAAAATAAC CATTCGAAGG
    AAGAAGAAAG TTCAGATCCC AGTAAATCGT CCTGATCCTG AGCCTGTGTC TGAGAACGAA
    GAGGACAGTT ACGACGAGGA AGCGCATGAC CCGAGAAGTG GCCGTGGGGC CCTGGCAAAC
    AGGAGGAGCG AGAAGAGCTG GGGCCGAGAT AGGAGTGCCA GTCGGGAGAG GAGTCTGTCC
    CCGCGGTCTG ACAGGCGGTC TGTAGCCTCC AGTCAGCCTG CTAGACCCAC CAAAGTCACC
    CTGGTGAAGT CCCGGAAGAA TGAAGAATAT GGTCTTCGAT TGGCCAGCCA TATATTTGTG
    AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT
    GTATTGAAGA TAAATGGTAC CGTGACGGAA AACATGTCAC TGACAGATGC GAAGACGCTG
    ATCGAGAGGT CTAAGGGCAA GTTAAAGATG GTGGTTCAGA GAGATGAGCG GGCTACCCTG
    CTGAATGTCC CTGACCTTTC TGACAGCATC CACTCTGCTG ACGCCTCCGA GAGGGACGAT
    ATTTCAGAAA TCCAGTCACT GGCATCAGAC CATTCAGGTC GATCCCACGA CCGGCCTCCC
    CGTCACAGCC GCTCTCGGTC TCCTGACCAG CGGTCCGAGC CTTCTGACCA TTCTAGACAC
    TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA
    CCAGGGGCTG TCTCGACTCC GGTAAAGCAT GCAGATGATC ATACACCTAA AACAGTGGAA
    GAAGTTGTAG TTGAGCGAAA TGAGAAACAA GCACCCAATC TTCCAGAACC AAAGCCTGTG
    TACGCACAGG TAGGACAGCC AGACGTGGAT TTACCTGTCA GTCCATCTGA TGGCGTCCTG
    CCCAACTCAA CTCATGAAGA TGGCATTCTT CGGCCCAGCA TGAAGTTAGT AAAATTCAGG
    AAAGGAGATA GTGTGGGTCT TCGACTAGCT GGTGGAAACG ATGTTGGCAT ATTTGTAGCT
    GGTGTTCTCG AAGATAGCCC TGCTGCCAAA GAAGGCTTGG AGGAGGGCGA CCAGATCCTC
    AGGGTAAACA ATGTAGATTT CACAAATATC ATAAGAGAAG AAGCCGTCCT CTTCCTGCTT
    GACCTCCCAA AAGGAGAAGA AGTGACCATA TTGGCTCAGA AGAAGAAAGA TGTTTATCGT
    CGCATCGTAG AATCCGATGT GGGTGATTCT TTCTATATCA GAACCCATTT TGAATATGAG
    AAGGAATCTC CCTATGGACT CAGTTTTAAC AAAGGAGAGG TGTTCCGTGT CGTGGACACT
    CTGTACAATG GGAAGCTGGG GTCCTGGCTT GCCATTCGGA TTGGCAAAAA CCATAAAGAG
    GTGGAACGTG GCATCATCCC AAACAAAAAC AGAGCTGAGC AGTTAGCCAG TGTGCAGTAT
    ACACTTCCCA AGACAGCAGG GGGAGACCGC GCCGACTTCT GGAGGTTCCG CGGTCTGCGC
    AGCTCCAAGA GAAACCTGCG CAAGAGCCGA GAAGACCTCT CAGCCCAGCC AGTCCAGACC
    AAGTTCCCGG CGTATGAGAG GGTGGTTCTT CGAGAAGCTG GATTCCTGAG GCCTGTAACC
    ATCTTTGGAC CAATAGCTGA TGTTGCCAGG GAGAAACTGG CAAGAGAGGA GCCAGATATT
    TACCAGATCG CAAAAAGTGA ACCTCGGGAT GCAGGAACCG ACCAGCGCAG CTCTGGCATT
    ATTCGCCTCC ACACAATAAA GCAGATAATA GATCAGGACA AACATGCCTT ACTGGATGTA
    ACGCCCAATG CAGTAGACCG TCTGAACTAT GCCCAGTGGT ATCCAATCGT GGTGTTCCTT
    AACCCGGACT CAAAGCAGGG TGTGAAAACA ATGCGAATGA GGTTATGTCC AGAATCTCGG
    AAAAGCGCCA GGAAGTTATA TGAGCGCTCT CATAAACTTC GTAAAAATAA TCACCATCTT
    TTTACAACTA CAATTAACTT AAATTCAATG AATGATGGCT GGTATGGTGC ACTGAAAGAA
    GCAATCCAAC AACAACAGAA CCAGTTGGTA TGGGTTTCTG AGGGAAAGGC GGATGGTGCT
    ACAAGTGATG ACCTTGATTT GCATGATGAT CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT
    GAATACTCAA TGTATAGCAC GGACAGTAGA CACACTTCTG ACTATGAAGA CACAGACACA
    GAAGGCGGGG CCTACACTGA TCAAGAACTA GATGAGACCC TAAATGATGA GGTGGGGACT
    CCCCCGGAGT CTGCCATTAC CCGGTCCTCT GAGCCTGTCA GAGAGGACTC CTCTGGAATG
    CATCCTGACC ACCAGGCATA TCCTCCCTAC TCACCACAAG CGCAGCCACA GCCAGGCCAT
    AGAATAGACT CCCCTGGATT TAAAACAGCC TCTCAACAGA AAGCAGAAGC CTCATCTCCA
    GGCCCTTACC TTTCGCCTGA AACAAACCCG GCACCATCAG CCTCTGCTGT TAATCCTCAC
    GTAGACTTAA CTAATGTCAG ACTGGAGGAG CCGAGCCCAG CTCCCCCCTC CTCTGACTCA
    CCACACGCCG ACTCTTTACG AGCTCCAGGC ACTGAGGCCG CTCACGTGCA GCTCAAAGGT
    CCAGAGCCGT CCCTGTCGTC GCATGTGGGC CCAGCAAAGG TGTACAGGAA GGACCCCTAC
    CCTGAGGAGA TGATGAGACA GAACCACGTC TTGAAGCAGC CGGCTGTGGT TCACCCTGGG
    CAGAGGCCAG ACAAGGAGCA AGGCCTGAGC TACGAGCCGC AGCCTCCGTA CACAGAGCGA
    CAGGCTGGCA GGGAGTTGGA GCGGCCTGCA TACAGATACG ATGCCTCTAG CTACCCGGAG
    CAGTTTGCTC GGAACTATGA CCATCGTCTG CGCTATGACG ACCGCCTCCT CACCTATGAG
    GAACAATGGC CCTACTACGA TGACAAGCAG CCTTACCAGC CCCGGCCTCT TGATGGTCAG
    CCTCCCGCGG AGCCAACAGA ACGAGGGTAT TTCCCCCGTT TTGAGGAGCC AGTGCCCCTG
    TCCTATGAAG GCCGAGCGCA CTACGACCCA CTGCCCAGGA CCTCCACCTT GCGGCACGAA
    GAGCCTCCCG CCCCTGCCCC TGGGTACGAC GTGCACGGAA GGTACCGGCC TGACGTGCAG
    CCCTACTCCA CTGCTGGCCC CAAGGTGCCC CCTGAGCCCA AGCAGTACTT GGACCAGTAC
    CCTCGGAGTT ACGAGCCAGC GCCAGCCCAG GGCTTCCCCT CCAGGGCAGG CCCAGGCCAC
    TACGAGCCTC TTCACAGCAC CACGCTCGCT CCACCACTTG GCCCCTCGTC CCAGCACAAG
    CCCGAAGTCC CGCCCACAGG CACCAAACCC CTGCCTCCAC CCCCAGCTCC GGCCGAGGAG
    GAGGAAGACC CAGCCATGAA GCCACAATCC GTGCTCACCA GAGTGAAGAT GTTTGAGAAT
    AAGAGGTCTG CTTCCATGGA GAACAGGAAG GATGAGAGCC ATTCCACTAG TTTCAAGCCC
    CCAGAAGTCA CGTCAAAACC GGCAGGTGCT CCTGTCCCAG GTCCCAAAGC CCCTGCTCCG
    AGTCAGCTCG GTGAACACGA CAGAGCAGCA CATAGGATCC CTGAACCACA GAGGCCTCAC
    ATGAAGCCAC CTGAAGACAT TGTTCGGGCA AATCACTATG ACCCCGAGGA GGATGAGGAG
    TACTACCGGA AGCAGCTCTC CTACTTCGAT CGGAGAAGCT TTGAGGCCAA GCCCCCCACC
    CACATCCCCA CTAGCCACCT CTCCGAGCCC ACCAGGCCGG CTCACCCCCA ATCTCAGCCA
    CAGTTTGCCG GTTACTCTTC CAAGGGGAAG GCTGCTGAGG CCGAAGCTGT GGACAGGTCA
    TTTGGGGAGA AGCGCTATGA CCCGCTGCAG GCGACGCCCC CCCCTCCGCC GCTGCCTGTT
    CAGTACAGCC AGGGTGCTCA ACCCAGCCCC AACACGGCTC TTGGCCTCCA CGCACACACC
    AAGGGGGCGC ATGGTGAAGG TAATGCCATG TCGTTGGATT TTCAGAACTC GTTAGTGTCC
    AAACCGGACC CACCTCCATC TCAGAATAAG CCAGCAGCTT ACAGACCAGC CCCTCGAGAG
    GATGCTGTCC AGTCTGGCTT CTACCCACAA AAGAGTTTCC CAGAGAAAGC CCCAGCTAAT
    GGGGTCGAGC AGACGCAGAA AACCGTCACG CCAGCATACA ATCGATTCAC ACCAAAACCA
    TACACAAGCT CTGCACGGCC ATTTGAACGC AAGTTTGAAA GCCCTAAATT CAACCACAAT
    CTCTTGCCGA ATGAAACCGC ACACAAACCT GACCTGTCCT CCAAAGCCCC CGCTTCTCCG
    AAAACCCTCC TGAAGTCTGC ACAGCCTCCT GAGTTTGACA GTGGCGTGGA GACCCTCTCT
    GTCCACACTG ACAACAAGCT GAAATACCAA GTAAACAGTG TTAGCATGGG GCCGAGAGCA
    GTGCCCGTGA GTCCCTCAGC CGTGGAGGAA GATGAAGACG AAGACGGTCA CACAGTGGTG
    GCTACTGCCC GCGGCGTGTT TAACAGCAAT GGTGGGGTGC TGAGCTCCAT TGAGACCGGT
    GTCAGCATCA TCATCCCCCA GGGAGCCATT CCCGAGGGGG TGGAACAGGA GATCTACTTC
    AAGGTCTGCC GCGACAACAG CATCCTGCCG CCTTTGGACA AAGAGAAGGG TGAGACCCTG
    CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG
    CGCCTACCAC ACTGTGCGTC CATGACTCCT GACGGTTGGT CTTTTGCTCT AAAATCATCC
    GACTCCTCGT CGGGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCAAAT
    TATCTTGTTG GAGCAAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228432
    Clone ID Related Accession (Same CDS sequence) XM_024982003.1
    Accession Version XM_024982003.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5529bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGGGCCC TGGCCGCGAG 
    CGGAGCGGGG ACAAGATGAA GTACCAGAAG TACCTGACGG TGCTGCAGAT GGCCATCGGC
    GTCACGCCGT CCAACCGCGG CAGCCTGCTG CCGCTCAAGA GGAGGCTGTG GGTAACGCCG
    TCCTCTGAAA ACCCCAGTGG TGCTACTTCT AGTGTCAGCC AAGGAAAGCC TTCTTTAAGA
    CGAATTAAAG GGAGACTGCA CAGGAGCAAG AGCCTCGATA GCATCGATTT CTGTGAACTC
    ACTAACACAA CAATGGAGGA AACAGCTATA TGGGAACAAC ACACGGTGAC ACTTCACAGG
    GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC ACATTTCCAG
    AGCGGGGAAA CCTCCATCGT GATTTCAGAT GTGCTGAAGG GGGGGCCTGC GGAAGGACAG
    CTGCAGGAAA ATGACCGGGT GGCAATGGTT AATGGAGTCT CCATGGATAA TGTTGAGCAT
    GCGTTTGCTG TTCAGCAACT AAGGAAAAGT GGGAAAAATG CAAAAATAAC CATTCGAAGG
    AAGAAGAAAG TTCAGATCCC AGTAAATCGT CCTGATCCTG AGCCTGTGTC TGAGAACGAA
    GAGGACAGTT ACGACGAGGA AGCGCATGAC CCGAGAAGTG GCCGTGGGGC CCTGGCAAAC
    AGGAGGAGCG AGAAGAGCTG GGGCCGAGAT AGGAGTGCCA GTCGGGAGAG GAGTCTGTCC
    CCGCGGTCTG ACAGGCGGTC TGTAGCCTCC AGTCAGCCTG CTAGACCCAC CAAAGTCACC
    CTGGTGAAGT CCCGGAAGAA TGAAGAATAT GGTCTTCGAT TGGCCAGCCA TATATTTGTG
    AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT
    GTATTGAAGA TAAATGGTAC CGTGACGGAA AACATGTCAC TGACAGATGC GAAGACGCTG
    ATCGAGAGGT CTAAGGGCAA GTTAAAGATG GTGGTTCAGA GAGATGAGCG GGCTACCCTG
    CTGAATGTCC CTGACCTTTC TGACAGCATC CACTCTGCTG ACGCCTCCGA GAGGGACGAT
    ATTTCAGAAA TCCAGTCACT GGCATCAGAC CATTCAGGTC GATCCCACGA CCGGCCTCCC
    CGTCACAGCC GCTCTCGGTC TCCTGACCAG CGGTCCGAGC CTTCTGACCA TTCTAGACAC
    TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA
    CCAGGGGCTG TCTCGACTCC GGTAAAGCAT GCAGATGATC ATACACCTAA AACAGTGGAA
    GAAGTTGTAG TTGAGCGAAA TGAGAAACAA GCACCCAATC TTCCAGAACC AAAGCCTGTG
    TACGCACAGG TAGGACAGCC AGACGTGGAT TTACCTGTCA GTCCATCTGA TGGCGTCCTG
    CCCAACTCAA CTCATGAAGA TGGCATTCTT CGGCCCAGCA TGAAGTTAGT AAAATTCAGG
    AAAGGAGATA GTGTGGGTCT TCGACTAGCT GGTGGAAACG ATGTTGGCAT ATTTGTAGCT
    GGTGTTCTCG AAGATAGCCC TGCTGCCAAA GAAGGCTTGG AGGAGGGCGA CCAGATCCTC
    AGGGTAAACA ATGTAGATTT CACAAATATC ATAAGAGAAG AAGCCGTCCT CTTCCTGCTT
    GACCTCCCAA AAGGAGAAGA AGTGACCATA TTGGCTCAGA AGAAGAAAGA TGTTTATCGT
    CGCATCGTAG AATCCGATGT GGGTGATTCT TTCTATATCA GAACCCATTT TGAATATGAG
    AAGGAATCTC CCTATGGACT CAGTTTTAAC AAAGGAGAGG TGTTCCGTGT CGTGGACACT
    CTGTACAATG GGAAGCTGGG GTCCTGGCTT GCCATTCGGA TTGGCAAAAA CCATAAAGAG
    GTGGAACGTG GCATCATCCC AAACAAAAAC AGAGCTGAGC AGTTAGCCAG TGTGCAGTAT
    ACACTTCCCA AGACAGCAGG GGGAGACCGC GCCGACTTCT GGAGGTTCCG CGGTCTGCGC
    AGCTCCAAGA GAAACCTGCG CAAGAGCCGA GAAGACCTCT CAGCCCAGCC AGTCCAGACC
    AAGTTCCCGG CGTATGAGAG GGTGGTTCTT CGAGAAGCTG GATTCCTGAG GCCTGTAACC
    ATCTTTGGAC CAATAGCTGA TGTTGCCAGG GAGAAACTGG CAAGAGAGGA GCCAGATATT
    TACCAGATCG CAAAAAGTGA ACCTCGGGAT GCAGGAACCG ACCAGCGCAG CTCTGGCATT
    ATTCGCCTCC ACACAATAAA GCAGATAATA GATCAGGACA AACATGCCTT ACTGGATGTA
    ACGCCCAATG CAGTAGACCG TCTGAACTAT GCCCAGTGGT ATCCAATCGT GGTGTTCCTT
    AACCCGGACT CAAAGCAGGG TGTGAAAACA ATGCGAATGA GGTTATGTCC AGAATCTCGG
    AAAAGCGCCA GGAAGTTATA TGAGCGCTCT CATAAACTTC GTAAAAATAA TCACCATCTT
    TTTACAACTA CAATTAACTT AAATTCAATG AATGATGGCT GGTATGGTGC ACTGAAAGAA
    GCAATCCAAC AACAACAGAA CCAGTTGGTA TGGGTTTCTG AGGGAAAGGC GGATGGTGCT
    ACAAGTGATG ACCTTGATTT GCATGATGAT CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT
    GAATACTCAA TGTATAGCAC GGACAGTAGA CACACTTCTG ACTATGAAGA CACAGACACA
    GAAGGCGGGG CCTACACTGA TCAAGAACTA GATGAGACCC TAAATGATGA GGTGGGGACT
    CCCCCGGAGT CTGCCATTAC CCGGTCCTCT GAGCCTGTCA GAGAGGACTC CTCTGGAATG
    CATCCTGACC ACCAGGCATA TCCTCCCTAC TCACCACAAG CGCAGCCACA GCCAGGCCAT
    AGAATAGACT CCCCTGGATT TAAAACAGCC TCTCAACAGA AAGCAGAAGC CTCATCTCCA
    GGCCCTTACC TTTCGCCTGA AACAAACCCG GCACCATCAG CCTCTGCTGT TAATCCTCAC
    GTAGACTTAA CTAATGTCAG ACTGGAGGAG CCGAGCCCAG CTCCCCCCTC CTCTGACTCA
    CCACACGCCG ACTCTTTACG AGCTCCAGGC ACTGAGGCCG CTCACGTGCA GCTCAAAGGT
    CCAGAGCCGT CCCTGTCGTC GCATGTGGGC CCAGCAAAGG TGTACAGGAA GGACCCCTAC
    CCTGAGGAGA TGATGAGACA GAACCACGTC TTGAAGCAGC CGGCTGTGGT TCACCCTGGG
    CAGAGGCCAG ACAAGGAGCA AGGCCTGAGC TACGAGCCGC AGCCTCCGTA CACAGAGCGA
    CAGGCTGGCA GGGAGTTGGA GCGGCCTGCA TACAGATACG ATGCCTCTAG CTACCCGGAG
    CAGTTTGCTC GGAACTATGA CCATCGTCTG CGCTATGACG ACCGCCTCCT CACCTATGAG
    GAACAATGGC CCTACTACGA TGACAAGCAG CCTTACCAGC CCCGGCCTCT TGATGGTCAG
    CCTCCCGCGG AGCCAACAGA ACGAGGGTAT TTCCCCCGTT TTGAGGAGCC AGTGCCCCTG
    TCCTATGAAG GCCGAGCGCA CTACGACCCA CTGCCCAGGA CCTCCACCTT GCGGCACGAA
    GAGCCTCCCG CCCCTGCCCC TGGGTACGAC GTGCACGGAA GGTACCGGCC TGACGTGCAG
    CCCTACTCCA CTGCTGGCCC CAAGGTGCCC CCTGAGCCCA AGCAGTACTT GGACCAGTAC
    CCTCGGAGTT ACGAGCCAGC GCCAGCCCAG GGCTTCCCCT CCAGGGCAGG CCCAGGCCAC
    TACGAGCCTC TTCACAGCAC CACGCTCGCT CCACCACTTG GCCCCTCGTC CCAGCACAAG
    CCCGAAGTCC CGCCCACAGG CACCAAACCC CTGCCTCCAC CCCCAGCTCC GGCCGAGGAG
    GAGGAAGACC CAGCCATGAA GCCACAATCC GTGCTCACCA GAGTGAAGAT GTTTGAGAAT
    AAGAGGTCTG CTTCCATGGA GAACAGGAAG GATGAGAGCC ATTCCACTAG TTTCAAGCCC
    CCAGAAGTCA CGTCAAAACC GGCAGGTGCT CCTGTCCCAG GTCCCAAAGC CCCTGCTCCG
    AGTCAGCTCG GTGAACACGA CAGAGCAGCA CATAGGATCC CTGAACCACA GAGGCCTCAC
    ATGAAGCCAC CTGAAGACAT TGTTCGGGCA AATCACTATG ACCCCGAGGA GGATGAGGAG
    TACTACCGGA AGCAGCTCTC CTACTTCGAT CGGAGAAGCT TTGAGGCCAA GCCCCCCACC
    CACATCCCCA CTAGCCACCT CTCCGAGCCC ACCAGGCCGG CTCACCCCCA ATCTCAGCCA
    CAGTTTGCCG GTTACTCTTC CAAGGGGAAG GCTGCTGAGG CCGAAGCTGT GGACAGGTCA
    TTTGGGGAGA AGCGCTATGA CCCGCTGCAG GCGACGCCCC CCCCTCCGCC GCTGCCTGTT
    CAGTACAGCC AGGGTGCTCA ACCCAGCCCC AACACGGCTC TTGGCCTCCA CGCACACACC
    AAGGGGGCGC ATGGTGAAGG TAATGCCATG TCGTTGGATT TTCAGAACTC GTTAGTGTCC
    AAACCGGACC CACCTCCATC TCAGAATAAG CCAGCAGCTT ACAGACCAGC CCCTCGAGAG
    GATGCTGTCC AGTCTGGCTT CTACCCACAA AAGAGTTTCC CAGAGAAAGC CCCAGCTAAT
    GGGGTCGAGC AGACGCAGAA AACCGTCACG CCAGCATACA ATCGATTCAC ACCAAAACCA
    TACACAAGCT CTGCACGGCC ATTTGAACGC AAGTTTGAAA GCCCTAAATT CAACCACAAT
    CTCTTGCCGA ATGAAACCGC ACACAAACCT GACCTGTCCT CCAAAGCCCC CGCTTCTCCG
    AAAACCCTCC TGAAGTCTGC ACAGCCTCCT GAGTTTGACA GTGGCGTGGA GACCCTCTCT
    GTCCACACTG ACAACAAGCT GAAATACCAA GTAAACAGTG TTAGCATGGG GCCGAGAGCA
    GTGCCCGTGA GTCCCTCAGC CGTGGAGGAA GATGAAGACG AAGACGGTCA CACAGTGGTG
    GCTACTGCCC GCGGCGTGTT TAACAGCAAT GGTGGGGTGC TGAGCTCCAT TGAGACCGGT
    GTCAGCATCA TCATCCCCCA GGGAGCCATT CCCGAGGGGG TGGAACAGGA GATCTACTTC
    AAGGTCTGCC GCGACAACAG CATCCTGCCG CCTTTGGACA AAGAGAAGGG TGAGACCCTG
    CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG
    CGCCTACCAC ACTGTGCGTC CATGACTCCT GACGGTGATC CTAAAACCTG GCAAAACAAG
    TGTCTTCCTG GAGATCCAAA TTATCTTGTT GGAGCAAACT GTGTTTCTGT CCTGATTGAC
    CACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837771.1
    CDS50..5578
    Translation

    Target ORF information:

    RefSeq Version XM_024982003.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 1 (TJP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982003.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGGGCCC TGGCCGCGAG 
    CGGAGCGGGG ACAAGATGAA GTACCAGAAG TACCTGACGG TGCTGCAGAT GGCCATCGGC
    GTCACGCCGT CCAACCGCGG CAGCCTGCTG CCGCTCAAGA GGAGGCTGTG GGTAACGCCG
    TCCTCTGAAA ACCCCAGTGG TGCTACTTCT AGTGTCAGCC AAGGAAAGCC TTCTTTAAGA
    CGAATTAAAG GGAGACTGCA CAGGAGCAAG AGCCTCGATA GCATCGATTT CTGTGAACTC
    ACTAACACAA CAATGGAGGA AACAGCTATA TGGGAACAAC ACACGGTGAC ACTTCACAGG
    GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC ACATTTCCAG
    AGCGGGGAAA CCTCCATCGT GATTTCAGAT GTGCTGAAGG GGGGGCCTGC GGAAGGACAG
    CTGCAGGAAA ATGACCGGGT GGCAATGGTT AATGGAGTCT CCATGGATAA TGTTGAGCAT
    GCGTTTGCTG TTCAGCAACT AAGGAAAAGT GGGAAAAATG CAAAAATAAC CATTCGAAGG
    AAGAAGAAAG TTCAGATCCC AGTAAATCGT CCTGATCCTG AGCCTGTGTC TGAGAACGAA
    GAGGACAGTT ACGACGAGGA AGCGCATGAC CCGAGAAGTG GCCGTGGGGC CCTGGCAAAC
    AGGAGGAGCG AGAAGAGCTG GGGCCGAGAT AGGAGTGCCA GTCGGGAGAG GAGTCTGTCC
    CCGCGGTCTG ACAGGCGGTC TGTAGCCTCC AGTCAGCCTG CTAGACCCAC CAAAGTCACC
    CTGGTGAAGT CCCGGAAGAA TGAAGAATAT GGTCTTCGAT TGGCCAGCCA TATATTTGTG
    AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT
    GTATTGAAGA TAAATGGTAC CGTGACGGAA AACATGTCAC TGACAGATGC GAAGACGCTG
    ATCGAGAGGT CTAAGGGCAA GTTAAAGATG GTGGTTCAGA GAGATGAGCG GGCTACCCTG
    CTGAATGTCC CTGACCTTTC TGACAGCATC CACTCTGCTG ACGCCTCCGA GAGGGACGAT
    ATTTCAGAAA TCCAGTCACT GGCATCAGAC CATTCAGGTC GATCCCACGA CCGGCCTCCC
    CGTCACAGCC GCTCTCGGTC TCCTGACCAG CGGTCCGAGC CTTCTGACCA TTCTAGACAC
    TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA
    CCAGGGGCTG TCTCGACTCC GGTAAAGCAT GCAGATGATC ATACACCTAA AACAGTGGAA
    GAAGTTGTAG TTGAGCGAAA TGAGAAACAA GCACCCAATC TTCCAGAACC AAAGCCTGTG
    TACGCACAGG TAGGACAGCC AGACGTGGAT TTACCTGTCA GTCCATCTGA TGGCGTCCTG
    CCCAACTCAA CTCATGAAGA TGGCATTCTT CGGCCCAGCA TGAAGTTAGT AAAATTCAGG
    AAAGGAGATA GTGTGGGTCT TCGACTAGCT GGTGGAAACG ATGTTGGCAT ATTTGTAGCT
    GGTGTTCTCG AAGATAGCCC TGCTGCCAAA GAAGGCTTGG AGGAGGGCGA CCAGATCCTC
    AGGGTAAACA ATGTAGATTT CACAAATATC ATAAGAGAAG AAGCCGTCCT CTTCCTGCTT
    GACCTCCCAA AAGGAGAAGA AGTGACCATA TTGGCTCAGA AGAAGAAAGA TGTTTATCGT
    CGCATCGTAG AATCCGATGT GGGTGATTCT TTCTATATCA GAACCCATTT TGAATATGAG
    AAGGAATCTC CCTATGGACT CAGTTTTAAC AAAGGAGAGG TGTTCCGTGT CGTGGACACT
    CTGTACAATG GGAAGCTGGG GTCCTGGCTT GCCATTCGGA TTGGCAAAAA CCATAAAGAG
    GTGGAACGTG GCATCATCCC AAACAAAAAC AGAGCTGAGC AGTTAGCCAG TGTGCAGTAT
    ACACTTCCCA AGACAGCAGG GGGAGACCGC GCCGACTTCT GGAGGTTCCG CGGTCTGCGC
    AGCTCCAAGA GAAACCTGCG CAAGAGCCGA GAAGACCTCT CAGCCCAGCC AGTCCAGACC
    AAGTTCCCGG CGTATGAGAG GGTGGTTCTT CGAGAAGCTG GATTCCTGAG GCCTGTAACC
    ATCTTTGGAC CAATAGCTGA TGTTGCCAGG GAGAAACTGG CAAGAGAGGA GCCAGATATT
    TACCAGATCG CAAAAAGTGA ACCTCGGGAT GCAGGAACCG ACCAGCGCAG CTCTGGCATT
    ATTCGCCTCC ACACAATAAA GCAGATAATA GATCAGGACA AACATGCCTT ACTGGATGTA
    ACGCCCAATG CAGTAGACCG TCTGAACTAT GCCCAGTGGT ATCCAATCGT GGTGTTCCTT
    AACCCGGACT CAAAGCAGGG TGTGAAAACA ATGCGAATGA GGTTATGTCC AGAATCTCGG
    AAAAGCGCCA GGAAGTTATA TGAGCGCTCT CATAAACTTC GTAAAAATAA TCACCATCTT
    TTTACAACTA CAATTAACTT AAATTCAATG AATGATGGCT GGTATGGTGC ACTGAAAGAA
    GCAATCCAAC AACAACAGAA CCAGTTGGTA TGGGTTTCTG AGGGAAAGGC GGATGGTGCT
    ACAAGTGATG ACCTTGATTT GCATGATGAT CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT
    GAATACTCAA TGTATAGCAC GGACAGTAGA CACACTTCTG ACTATGAAGA CACAGACACA
    GAAGGCGGGG CCTACACTGA TCAAGAACTA GATGAGACCC TAAATGATGA GGTGGGGACT
    CCCCCGGAGT CTGCCATTAC CCGGTCCTCT GAGCCTGTCA GAGAGGACTC CTCTGGAATG
    CATCCTGACC ACCAGGCATA TCCTCCCTAC TCACCACAAG CGCAGCCACA GCCAGGCCAT
    AGAATAGACT CCCCTGGATT TAAAACAGCC TCTCAACAGA AAGCAGAAGC CTCATCTCCA
    GGCCCTTACC TTTCGCCTGA AACAAACCCG GCACCATCAG CCTCTGCTGT TAATCCTCAC
    GTAGACTTAA CTAATGTCAG ACTGGAGGAG CCGAGCCCAG CTCCCCCCTC CTCTGACTCA
    CCACACGCCG ACTCTTTACG AGCTCCAGGC ACTGAGGCCG CTCACGTGCA GCTCAAAGGT
    CCAGAGCCGT CCCTGTCGTC GCATGTGGGC CCAGCAAAGG TGTACAGGAA GGACCCCTAC
    CCTGAGGAGA TGATGAGACA GAACCACGTC TTGAAGCAGC CGGCTGTGGT TCACCCTGGG
    CAGAGGCCAG ACAAGGAGCA AGGCCTGAGC TACGAGCCGC AGCCTCCGTA CACAGAGCGA
    CAGGCTGGCA GGGAGTTGGA GCGGCCTGCA TACAGATACG ATGCCTCTAG CTACCCGGAG
    CAGTTTGCTC GGAACTATGA CCATCGTCTG CGCTATGACG ACCGCCTCCT CACCTATGAG
    GAACAATGGC CCTACTACGA TGACAAGCAG CCTTACCAGC CCCGGCCTCT TGATGGTCAG
    CCTCCCGCGG AGCCAACAGA ACGAGGGTAT TTCCCCCGTT TTGAGGAGCC AGTGCCCCTG
    TCCTATGAAG GCCGAGCGCA CTACGACCCA CTGCCCAGGA CCTCCACCTT GCGGCACGAA
    GAGCCTCCCG CCCCTGCCCC TGGGTACGAC GTGCACGGAA GGTACCGGCC TGACGTGCAG
    CCCTACTCCA CTGCTGGCCC CAAGGTGCCC CCTGAGCCCA AGCAGTACTT GGACCAGTAC
    CCTCGGAGTT ACGAGCCAGC GCCAGCCCAG GGCTTCCCCT CCAGGGCAGG CCCAGGCCAC
    TACGAGCCTC TTCACAGCAC CACGCTCGCT CCACCACTTG GCCCCTCGTC CCAGCACAAG
    CCCGAAGTCC CGCCCACAGG CACCAAACCC CTGCCTCCAC CCCCAGCTCC GGCCGAGGAG
    GAGGAAGACC CAGCCATGAA GCCACAATCC GTGCTCACCA GAGTGAAGAT GTTTGAGAAT
    AAGAGGTCTG CTTCCATGGA GAACAGGAAG GATGAGAGCC ATTCCACTAG TTTCAAGCCC
    CCAGAAGTCA CGTCAAAACC GGCAGGTGCT CCTGTCCCAG GTCCCAAAGC CCCTGCTCCG
    AGTCAGCTCG GTGAACACGA CAGAGCAGCA CATAGGATCC CTGAACCACA GAGGCCTCAC
    ATGAAGCCAC CTGAAGACAT TGTTCGGGCA AATCACTATG ACCCCGAGGA GGATGAGGAG
    TACTACCGGA AGCAGCTCTC CTACTTCGAT CGGAGAAGCT TTGAGGCCAA GCCCCCCACC
    CACATCCCCA CTAGCCACCT CTCCGAGCCC ACCAGGCCGG CTCACCCCCA ATCTCAGCCA
    CAGTTTGCCG GTTACTCTTC CAAGGGGAAG GCTGCTGAGG CCGAAGCTGT GGACAGGTCA
    TTTGGGGAGA AGCGCTATGA CCCGCTGCAG GCGACGCCCC CCCCTCCGCC GCTGCCTGTT
    CAGTACAGCC AGGGTGCTCA ACCCAGCCCC AACACGGCTC TTGGCCTCCA CGCACACACC
    AAGGGGGCGC ATGGTGAAGG TAATGCCATG TCGTTGGATT TTCAGAACTC GTTAGTGTCC
    AAACCGGACC CACCTCCATC TCAGAATAAG CCAGCAGCTT ACAGACCAGC CCCTCGAGAG
    GATGCTGTCC AGTCTGGCTT CTACCCACAA AAGAGTTTCC CAGAGAAAGC CCCAGCTAAT
    GGGGTCGAGC AGACGCAGAA AACCGTCACG CCAGCATACA ATCGATTCAC ACCAAAACCA
    TACACAAGCT CTGCACGGCC ATTTGAACGC AAGTTTGAAA GCCCTAAATT CAACCACAAT
    CTCTTGCCGA ATGAAACCGC ACACAAACCT GACCTGTCCT CCAAAGCCCC CGCTTCTCCG
    AAAACCCTCC TGAAGTCTGC ACAGCCTCCT GAGTTTGACA GTGGCGTGGA GACCCTCTCT
    GTCCACACTG ACAACAAGCT GAAATACCAA GTAAACAGTG TTAGCATGGG GCCGAGAGCA
    GTGCCCGTGA GTCCCTCAGC CGTGGAGGAA GATGAAGACG AAGACGGTCA CACAGTGGTG
    GCTACTGCCC GCGGCGTGTT TAACAGCAAT GGTGGGGTGC TGAGCTCCAT TGAGACCGGT
    GTCAGCATCA TCATCCCCCA GGGAGCCATT CCCGAGGGGG TGGAACAGGA GATCTACTTC
    AAGGTCTGCC GCGACAACAG CATCCTGCCG CCTTTGGACA AAGAGAAGGG TGAGACCCTG
    CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG
    CGCCTACCAC ACTGTGCGTC CATGACTCCT GACGGTGATC CTAAAACCTG GCAAAACAAG
    TGTCTTCCTG GAGATCCAAA TTATCTTGTT GGAGCAAACT GTGTTTCTGT CCTGATTGAC
    CACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228433
    Clone ID Related Accession (Same CDS sequence) XM_024982004.1
    Accession Version XM_024982004.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5508bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGGGCCC TGGCCGCGAG 
    CGGAGCGGGG ACAAGATGAA GTACCAGAAG TACCTGACGG TGCTGCAGAT GGCCATCGGC
    GTCACGCCGT CCAACCGCGG CAGCCTGCTG CCGCTCAAGA GGAGGCTGTG GGTAACGCCG
    TCCTCTGAAA ACCCCAGTGG TGCTACTTCT AGTGTCAGCC AAGGAAAGCC TTCTTTAAGA
    CGAATTAAAG GGAGACTGCA CAGGAGCAAG AGCCTCGATA GCATCGATTT CTGTGAACTC
    ACTAACACAA CAATGGAGGA AACAGCTATA TGGGAACAAC ACACGGTGAC ACTTCACAGG
    GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC ACATTTCCAG
    AGCGGGGAAA CCTCCATCGT GATTTCAGAT GTGCTGAAGG GGGGGCCTGC GGAAGGACAG
    CTGCAGGAAA ATGACCGGGT GGCAATGGTT AATGGAGTCT CCATGGATAA TGTTGAGCAT
    GCGTTTGCTG TTCAGCAACT AAGGAAAAGT GGGAAAAATG CAAAAATAAC CATTCGAAGG
    AAGAAGAAAG TTCAGATCCC AGTAAATCGT CCTGATCCTG AGCCTGTGTC TGAGAACGAA
    GAGGACAGTT ACGACGAGGA AGCGCATGAC CCGAGAAGTG GCCGTGGGGC CCTGGCAAAC
    AGGAGGAGCG AGAAGAGCTG GGGCCGAGAT AGGAGTGCCA GTCGGGAGAG GAGTCTGTCC
    CCGCGGTCTG ACAGGCGGTC TGTAGCCTCC AGTCAGCCTG CTAGACCCAC CAAAGTCACC
    CTGGTGAAGT CCCGGAAGAA TGAAGAATAT GGTCTTCGAT TGGCCAGCCA TATATTTGTG
    AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT
    GTATTGAAGA TAAATGGTAC CGTGACGGAA AACATGTCAC TGACAGATGC GAAGACGCTG
    ATCGAGAGGT CTAAGGGCAA GTTAAAGATG GTGGTTCAGA GAGATGAGCG GGCTACCCTG
    CTGAATGTCC CTGACCTTTC TGACAGCATC CACTCTGCTG ACGCCTCCGA GAGGGACGAT
    ATTTCAGAAA TCCAGTCACT GGCATCAGAC CATTCAGGTC GATCCCACGA CCGGCCTCCC
    CGTCACAGCC GCTCTCGGTC TCCTGACCAG CGGTCCGAGC CTTCTGACCA TTCTAGACAC
    TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA
    CCAGGGGCTG TCTCGACTCC GGTAAAGCAT GCAGATGATC ATACACCTAA AACAGTGGAA
    GAAGTTGTAG TTGAGCGAAA TGAGAAACAA GCACCCAATC TTCCAGAACC AAAGCCTGTG
    TACGCACAGG TAGGACAGCC AGACGTGGAT TTACCTGTCA GTCCATCTGA TGGCGTCCTG
    CCCAACTCAA CTCATGAAGA TGGCATTCTT CGGCCCAGCA TGAAGTTAGT AAAATTCAGG
    AAAGGAGATA GTGTGGGTCT TCGACTAGCT GGTGGAAACG ATGTTGGCAT ATTTGTAGCT
    GGTGTTCTCG AAGATAGCCC TGCTGCCAAA GAAGGCTTGG AGGAGGGCGA CCAGATCCTC
    AGGGTAAACA ATGTAGATTT CACAAATATC ATAAGAGAAG AAGCCGTCCT CTTCCTGCTT
    GACCTCCCAA AAGGAGAAGA AGTGACCATA TTGGCTCAGA AGAAGAAAGA TGTTTATCGT
    CGCATCGTAG AATCCGATGT GGGTGATTCT TTCTATATCA GAACCCATTT TGAATATGAG
    AAGGAATCTC CCTATGGACT CAGTTTTAAC AAAGGAGAGG TGTTCCGTGT CGTGGACACT
    CTGTACAATG GGAAGCTGGG GTCCTGGCTT GCCATTCGGA TTGGCAAAAA CCATAAAGAG
    GTGGAACGTG GCATCATCCC AAACAAAAAC AGAGCTGAGC AGTTAGCCAG TGTGCAGTAT
    ACACTTCCCA AGACAGCAGG GGGAGACCGC GCCGACTTCT GGAGGTTCCG CGGTCTGCGC
    AGCTCCAAGA GAAACCTGCG CAAGAGCCGA GAAGACCTCT CAGCCCAGCC AGTCCAGACC
    AAGTTCCCGG CGTATGAGAG GGTGGTTCTT CGAGAAGCTG GATTCCTGAG GCCTGTAACC
    ATCTTTGGAC CAATAGCTGA TGTTGCCAGG GAGAAACTGG CAAGAGAGGA GCCAGATATT
    TACCAGATCG CAAAAAGTGA ACCTCGGGAT GCAGGAACCG ACCAGCGCAG CTCTGGCATT
    ATTCGCCTCC ACACAATAAA GCAGATAATA GATCAGGACA AACATGCCTT ACTGGATGTA
    ACGCCCAATG CAGTAGACCG TCTGAACTAT GCCCAGTGGT ATCCAATCGT GGTGTTCCTT
    AACCCGGACT CAAAGCAGGG TGTGAAAACA ATGCGAATGA GGTTATGTCC AGAATCTCGG
    AAAAGCGCCA GGAAGTTATA TGAGCGCTCT CATAAACTTC GTAAAAATAA TCACCATCTT
    TTTACAACTA CAATTAACTT AAATTCAATG AATGATGGCT GGTATGGTGC ACTGAAAGAA
    GCAATCCAAC AACAACAGAA CCAGTTGGTA TGGGTTTCTG AGGGAAAGGC GGATGGTGCT
    ACAAGTGATG ACCTTGATTT GCATGATGAT CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT
    GAATACTCAA TGTATAGCAC GGACAGTAGA CACACTTCTG ACTATGAAGA CACAGACACA
    GAAGGCGGGG CCTACACTGA TCAAGAACTA GATGAGACCC TAAATGATGA GGTGGGGACT
    CCCCCGGAGT CTGCCATTAC CCGGTCCTCT GAGCCTGTCA GAGAGGACTC CTCTGGAATG
    CATCCTGACC ACCAGGCATA TCCTCCCTAC TCACCACAAG CGCAGCCACA GCCAGGCCAT
    AGAATAGACT CCCCTGGATT TAAAACAGCC TCTCAACAGA AAGCAGAAGC CTCATCTCCA
    GGCCCTTACC TTTCGCCTGA AACAAACCCG GCACCATCAG CCTCTGCTGT TAATCCTCAC
    GTAGACTTAA CTAATGTCAG ACTGGAGGAG CCGAGCCCAG CTCCCCCCTC CTCTGACTCA
    CCACACGCCG ACTCTTTACG AGCTCCAGGC ACTGAGGCCG CTCACGTGCA GCTCAAAGGT
    CCAGAGCCGT CCCTGTCGTC GCATGTGGGC CCAGCAAAGG TGTACAGGAA GGACCCCTAC
    CCTGAGGAGA TGATGAGACA GAACCACGTC TTGAAGCAGC CGGCTGTGGT TCACCCTGGG
    CAGAGGCCAG ACAAGGAGCA AGGCCTGAGC TACGAGCCGC AGCCTCCGTA CACAGAGCGA
    CAGGCTGGCA GGGAGTTGGA GCGGCCTGCA TACAGATACG ATGCCTCTAG CTACCCGGAG
    CAGTTTGCTC GGAACTATGA CCATCGTCTG CGCTATGACG ACCGCCTCCT CACCTATGAG
    GAACAATGGC CCTACTACGA TGACAAGCAG CCTTACCAGC CCCGGCCTCT TGATGGTCAG
    CCTCCCGCGG AGCCAACAGA ACGAGGGTAT TTCCCCCGTT TTGAGGAGCC AGTGCCCCTG
    TCCTATGAAG GCCGAGCGCA CTACGACCCA CTGCCCAGGA CCTCCACCTT GCGGCACGAA
    GAGCCTCCCG CCCCTGCCCC TGGGTACGAC GTGCACGGAA GGTACCGGCC TGACGTGCAG
    CCCTACTCCA CTGCTGGCCC CAAGGTGCCC CCTGAGCCCA AGCAGTACTT GGACCAGTAC
    CCTCGGAGTT ACGAGCCAGC GCCAGCCCAG GGCTTCCCCT CCAGGGCAGG CCCAGGCCAC
    TACGAGCCTC TTCACAGCAC CACGCTCGCT CCACCACTTG GCCCCTCGTC CCAGCACAAG
    CCCGAAGTCC CGCCCACAGG CACCAAACCC CTGCCTCCAC CCCCAGCTCC GGCCGAGGAG
    GAGGAAGACC CAGCCATGAA GCCACAATCC GTGCTCACCA GAGTGAAGAT GTTTGAGAAT
    AAGAGGTCTG CTTCCATGGA GAACAGGAAG GATGAGAGCC ATTCCACTAG TTTCAAGCCC
    CCAGAAGTCA CGTCAAAACC GGCAGGTGCT CCTGTCCCAG GTCCCAAAGC CCCTGCTCCG
    AGTCAGCTCG GTGAACACGA CAGAGCAGCA CATAGGATCC CTGAACCACA GAGGCCTCAC
    ATGAAGCCAC CTGAAGACAT TGTTCGGGCA AATCACTATG ACCCCGAGGA GGATGAGGAG
    TACTACCGGA AGCAGCTCTC CTACTTCGAT CGGAGAAGCT TTGAGGCCAA GCCCCCCACC
    CACATCCCCA CTAGCCACCT CTCCGAGCCC ACCAGGCCGG CTCACCCCCA ATCTCAGCCA
    CAGTTTGCCG GTTACTCTTC CAAGGGGAAG GCTGCTGAGG CCGAAGCTGT GGACAGGTCA
    TTTGGGGAGA AGCGCTATGA CCCGCTGCAG GCGACGCCCC CCCCTCCGCC GCTGCCTGTT
    CAGTACAGCC AGGGTGCTCA ACCCAGCCCC AACACGGCTC TTGGCCTCCA CGCACACACC
    AAGGGGGCGC ATGGTGAAGG TAATGCCATG TCGTTGGATT TTCAGAACTC GTTAGTGTCC
    AAACCGGACC CACCTCCATC TCAGAATAAG CCAGCAGCTT ACAGACCAGC CCCTCGAGAG
    GATGCTGTCC AGTCTGGCTT CTACCCACAA AAGAGTTTCC CAGAGAAAGC CCCAGCTAAT
    GGGGTCGAGC AGACGCAGAA AACCGTCACG CCAGCATACA ATCGATTCAC ACCAAAACCA
    TACACAAGCT CTGCACGGCC ATTTGAACGC AAGTTTGAAA GCCCTAAATT CAACCACAAT
    CTCTTGCCGA ATGAAACCGC ACACAAACCT GACCTGTCCT CCAAAGCCCC CGCTTCTCCG
    AAAACCCTCC TGAAGTCTGC ACAGCCTCCT GAGTTTGACA GTGGCGTGGA GACCCTCTCT
    GTCCACACTG ACAACAAGCT GAAATACCAA GTAAACAGTG TTAGCATGGG GCCGAGAGCA
    GTGCCCGTGA GTCCCTCAGC CGTGGAGGAA GATGAAGACG AAGACGGTCA CACAGTGGTG
    GCTACTGCCC GCGGCGTGTT TAACAGCAAT GGTGGGGTGC TGAGCTCCAT TGAGACCGGT
    GTCAGCATCA TCATCCCCCA GGGAGCCATT CCCGAGGGGG TGGAACAGGA GATCTACTTC
    AAGGTCTGCC GCGACAACAG CATCCTGCCG CCTTTGGACA AAGAGAAGGG TGAGACCCTG
    CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG
    CGCCTACCAC ACTGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCAAAT
    TATCTTGTTG GAGCAAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837772.1
    CDS50..5557
    Translation

    Target ORF information:

    RefSeq Version XM_024982004.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 1 (TJP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982004.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGGGCCC TGGCCGCGAG 
    CGGAGCGGGG ACAAGATGAA GTACCAGAAG TACCTGACGG TGCTGCAGAT GGCCATCGGC
    GTCACGCCGT CCAACCGCGG CAGCCTGCTG CCGCTCAAGA GGAGGCTGTG GGTAACGCCG
    TCCTCTGAAA ACCCCAGTGG TGCTACTTCT AGTGTCAGCC AAGGAAAGCC TTCTTTAAGA
    CGAATTAAAG GGAGACTGCA CAGGAGCAAG AGCCTCGATA GCATCGATTT CTGTGAACTC
    ACTAACACAA CAATGGAGGA AACAGCTATA TGGGAACAAC ACACGGTGAC ACTTCACAGG
    GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC ACATTTCCAG
    AGCGGGGAAA CCTCCATCGT GATTTCAGAT GTGCTGAAGG GGGGGCCTGC GGAAGGACAG
    CTGCAGGAAA ATGACCGGGT GGCAATGGTT AATGGAGTCT CCATGGATAA TGTTGAGCAT
    GCGTTTGCTG TTCAGCAACT AAGGAAAAGT GGGAAAAATG CAAAAATAAC CATTCGAAGG
    AAGAAGAAAG TTCAGATCCC AGTAAATCGT CCTGATCCTG AGCCTGTGTC TGAGAACGAA
    GAGGACAGTT ACGACGAGGA AGCGCATGAC CCGAGAAGTG GCCGTGGGGC CCTGGCAAAC
    AGGAGGAGCG AGAAGAGCTG GGGCCGAGAT AGGAGTGCCA GTCGGGAGAG GAGTCTGTCC
    CCGCGGTCTG ACAGGCGGTC TGTAGCCTCC AGTCAGCCTG CTAGACCCAC CAAAGTCACC
    CTGGTGAAGT CCCGGAAGAA TGAAGAATAT GGTCTTCGAT TGGCCAGCCA TATATTTGTG
    AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT
    GTATTGAAGA TAAATGGTAC CGTGACGGAA AACATGTCAC TGACAGATGC GAAGACGCTG
    ATCGAGAGGT CTAAGGGCAA GTTAAAGATG GTGGTTCAGA GAGATGAGCG GGCTACCCTG
    CTGAATGTCC CTGACCTTTC TGACAGCATC CACTCTGCTG ACGCCTCCGA GAGGGACGAT
    ATTTCAGAAA TCCAGTCACT GGCATCAGAC CATTCAGGTC GATCCCACGA CCGGCCTCCC
    CGTCACAGCC GCTCTCGGTC TCCTGACCAG CGGTCCGAGC CTTCTGACCA TTCTAGACAC
    TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA
    CCAGGGGCTG TCTCGACTCC GGTAAAGCAT GCAGATGATC ATACACCTAA AACAGTGGAA
    GAAGTTGTAG TTGAGCGAAA TGAGAAACAA GCACCCAATC TTCCAGAACC AAAGCCTGTG
    TACGCACAGG TAGGACAGCC AGACGTGGAT TTACCTGTCA GTCCATCTGA TGGCGTCCTG
    CCCAACTCAA CTCATGAAGA TGGCATTCTT CGGCCCAGCA TGAAGTTAGT AAAATTCAGG
    AAAGGAGATA GTGTGGGTCT TCGACTAGCT GGTGGAAACG ATGTTGGCAT ATTTGTAGCT
    GGTGTTCTCG AAGATAGCCC TGCTGCCAAA GAAGGCTTGG AGGAGGGCGA CCAGATCCTC
    AGGGTAAACA ATGTAGATTT CACAAATATC ATAAGAGAAG AAGCCGTCCT CTTCCTGCTT
    GACCTCCCAA AAGGAGAAGA AGTGACCATA TTGGCTCAGA AGAAGAAAGA TGTTTATCGT
    CGCATCGTAG AATCCGATGT GGGTGATTCT TTCTATATCA GAACCCATTT TGAATATGAG
    AAGGAATCTC CCTATGGACT CAGTTTTAAC AAAGGAGAGG TGTTCCGTGT CGTGGACACT
    CTGTACAATG GGAAGCTGGG GTCCTGGCTT GCCATTCGGA TTGGCAAAAA CCATAAAGAG
    GTGGAACGTG GCATCATCCC AAACAAAAAC AGAGCTGAGC AGTTAGCCAG TGTGCAGTAT
    ACACTTCCCA AGACAGCAGG GGGAGACCGC GCCGACTTCT GGAGGTTCCG CGGTCTGCGC
    AGCTCCAAGA GAAACCTGCG CAAGAGCCGA GAAGACCTCT CAGCCCAGCC AGTCCAGACC
    AAGTTCCCGG CGTATGAGAG GGTGGTTCTT CGAGAAGCTG GATTCCTGAG GCCTGTAACC
    ATCTTTGGAC CAATAGCTGA TGTTGCCAGG GAGAAACTGG CAAGAGAGGA GCCAGATATT
    TACCAGATCG CAAAAAGTGA ACCTCGGGAT GCAGGAACCG ACCAGCGCAG CTCTGGCATT
    ATTCGCCTCC ACACAATAAA GCAGATAATA GATCAGGACA AACATGCCTT ACTGGATGTA
    ACGCCCAATG CAGTAGACCG TCTGAACTAT GCCCAGTGGT ATCCAATCGT GGTGTTCCTT
    AACCCGGACT CAAAGCAGGG TGTGAAAACA ATGCGAATGA GGTTATGTCC AGAATCTCGG
    AAAAGCGCCA GGAAGTTATA TGAGCGCTCT CATAAACTTC GTAAAAATAA TCACCATCTT
    TTTACAACTA CAATTAACTT AAATTCAATG AATGATGGCT GGTATGGTGC ACTGAAAGAA
    GCAATCCAAC AACAACAGAA CCAGTTGGTA TGGGTTTCTG AGGGAAAGGC GGATGGTGCT
    ACAAGTGATG ACCTTGATTT GCATGATGAT CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT
    GAATACTCAA TGTATAGCAC GGACAGTAGA CACACTTCTG ACTATGAAGA CACAGACACA
    GAAGGCGGGG CCTACACTGA TCAAGAACTA GATGAGACCC TAAATGATGA GGTGGGGACT
    CCCCCGGAGT CTGCCATTAC CCGGTCCTCT GAGCCTGTCA GAGAGGACTC CTCTGGAATG
    CATCCTGACC ACCAGGCATA TCCTCCCTAC TCACCACAAG CGCAGCCACA GCCAGGCCAT
    AGAATAGACT CCCCTGGATT TAAAACAGCC TCTCAACAGA AAGCAGAAGC CTCATCTCCA
    GGCCCTTACC TTTCGCCTGA AACAAACCCG GCACCATCAG CCTCTGCTGT TAATCCTCAC
    GTAGACTTAA CTAATGTCAG ACTGGAGGAG CCGAGCCCAG CTCCCCCCTC CTCTGACTCA
    CCACACGCCG ACTCTTTACG AGCTCCAGGC ACTGAGGCCG CTCACGTGCA GCTCAAAGGT
    CCAGAGCCGT CCCTGTCGTC GCATGTGGGC CCAGCAAAGG TGTACAGGAA GGACCCCTAC
    CCTGAGGAGA TGATGAGACA GAACCACGTC TTGAAGCAGC CGGCTGTGGT TCACCCTGGG
    CAGAGGCCAG ACAAGGAGCA AGGCCTGAGC TACGAGCCGC AGCCTCCGTA CACAGAGCGA
    CAGGCTGGCA GGGAGTTGGA GCGGCCTGCA TACAGATACG ATGCCTCTAG CTACCCGGAG
    CAGTTTGCTC GGAACTATGA CCATCGTCTG CGCTATGACG ACCGCCTCCT CACCTATGAG
    GAACAATGGC CCTACTACGA TGACAAGCAG CCTTACCAGC CCCGGCCTCT TGATGGTCAG
    CCTCCCGCGG AGCCAACAGA ACGAGGGTAT TTCCCCCGTT TTGAGGAGCC AGTGCCCCTG
    TCCTATGAAG GCCGAGCGCA CTACGACCCA CTGCCCAGGA CCTCCACCTT GCGGCACGAA
    GAGCCTCCCG CCCCTGCCCC TGGGTACGAC GTGCACGGAA GGTACCGGCC TGACGTGCAG
    CCCTACTCCA CTGCTGGCCC CAAGGTGCCC CCTGAGCCCA AGCAGTACTT GGACCAGTAC
    CCTCGGAGTT ACGAGCCAGC GCCAGCCCAG GGCTTCCCCT CCAGGGCAGG CCCAGGCCAC
    TACGAGCCTC TTCACAGCAC CACGCTCGCT CCACCACTTG GCCCCTCGTC CCAGCACAAG
    CCCGAAGTCC CGCCCACAGG CACCAAACCC CTGCCTCCAC CCCCAGCTCC GGCCGAGGAG
    GAGGAAGACC CAGCCATGAA GCCACAATCC GTGCTCACCA GAGTGAAGAT GTTTGAGAAT
    AAGAGGTCTG CTTCCATGGA GAACAGGAAG GATGAGAGCC ATTCCACTAG TTTCAAGCCC
    CCAGAAGTCA CGTCAAAACC GGCAGGTGCT CCTGTCCCAG GTCCCAAAGC CCCTGCTCCG
    AGTCAGCTCG GTGAACACGA CAGAGCAGCA CATAGGATCC CTGAACCACA GAGGCCTCAC
    ATGAAGCCAC CTGAAGACAT TGTTCGGGCA AATCACTATG ACCCCGAGGA GGATGAGGAG
    TACTACCGGA AGCAGCTCTC CTACTTCGAT CGGAGAAGCT TTGAGGCCAA GCCCCCCACC
    CACATCCCCA CTAGCCACCT CTCCGAGCCC ACCAGGCCGG CTCACCCCCA ATCTCAGCCA
    CAGTTTGCCG GTTACTCTTC CAAGGGGAAG GCTGCTGAGG CCGAAGCTGT GGACAGGTCA
    TTTGGGGAGA AGCGCTATGA CCCGCTGCAG GCGACGCCCC CCCCTCCGCC GCTGCCTGTT
    CAGTACAGCC AGGGTGCTCA ACCCAGCCCC AACACGGCTC TTGGCCTCCA CGCACACACC
    AAGGGGGCGC ATGGTGAAGG TAATGCCATG TCGTTGGATT TTCAGAACTC GTTAGTGTCC
    AAACCGGACC CACCTCCATC TCAGAATAAG CCAGCAGCTT ACAGACCAGC CCCTCGAGAG
    GATGCTGTCC AGTCTGGCTT CTACCCACAA AAGAGTTTCC CAGAGAAAGC CCCAGCTAAT
    GGGGTCGAGC AGACGCAGAA AACCGTCACG CCAGCATACA ATCGATTCAC ACCAAAACCA
    TACACAAGCT CTGCACGGCC ATTTGAACGC AAGTTTGAAA GCCCTAAATT CAACCACAAT
    CTCTTGCCGA ATGAAACCGC ACACAAACCT GACCTGTCCT CCAAAGCCCC CGCTTCTCCG
    AAAACCCTCC TGAAGTCTGC ACAGCCTCCT GAGTTTGACA GTGGCGTGGA GACCCTCTCT
    GTCCACACTG ACAACAAGCT GAAATACCAA GTAAACAGTG TTAGCATGGG GCCGAGAGCA
    GTGCCCGTGA GTCCCTCAGC CGTGGAGGAA GATGAAGACG AAGACGGTCA CACAGTGGTG
    GCTACTGCCC GCGGCGTGTT TAACAGCAAT GGTGGGGTGC TGAGCTCCAT TGAGACCGGT
    GTCAGCATCA TCATCCCCCA GGGAGCCATT CCCGAGGGGG TGGAACAGGA GATCTACTTC
    AAGGTCTGCC GCGACAACAG CATCCTGCCG CCTTTGGACA AAGAGAAGGG TGAGACCCTG
    CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG
    CGCCTACCAC ACTGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCAAAT
    TATCTTGTTG GAGCAAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228434
    Clone ID Related Accession (Same CDS sequence) XM_024982005.1
    Accession Version XM_024982005.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5340bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGGGCCC TGGCCGCGAG 
    CGGAGCGGGG ACAAGATGAA GTACCAGAAG TACCTGACGG TGCTGCAGAT GGCCATCGGC
    GTCACGCCGT CCAACCGCGG CAGCCTGCTG CCGCTCAAGA GGAGGCTGTG GGTAACGCCG
    TCCTCTGAAA ACCCCAGTGG TGCTACTTCT AGTGTCAGCC AAGGAAAGCC TTCTTTAAGA
    CGAATTAAAG GGAGACTGCA CAGGAGCAAG AGCCTCGATA GCATCGATTT CTGTGAACTC
    ACTAACACAA CAATGGAGGA AACAGCTATA TGGGAACAAC ACACGGTGAC ACTTCACAGG
    GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC ACATTTCCAG
    AGCGGGGAAA CCTCCATCGT GATTTCAGAT GTGCTGAAGG GGGGGCCTGC GGAAGGACAG
    CTGCAGGAAA ATGACCGGGT GGCAATGGTT AATGGAGTCT CCATGGATAA TGTTGAGCAT
    GCGTTTGCTG TTCAGCAACT AAGGAAAAGT GGGAAAAATG CAAAAATAAC CATTCGAAGG
    AAGAAGAAAG TTCAGATCCC AGTAAATCGT CCTGATCCTG AGCCTGTGTC TGAGAACGAA
    GAGGACAGTT ACGACGAGGA AGCGCATGAC CCGAGAAGTG GCCGTGGGGC CCTGGCAAAC
    AGGAGGAGCG AGAAGAGCTG GGGCCGAGAT AGGAGTGCCA GTCGGGAGAG GAGTCTGTCC
    CCGCGGTCTG ACAGGCGGTC TGTAGCCTCC AGTCAGCCTG CTAGACCCAC CAAAGTCACC
    CTGGTGAAGT CCCGGAAGAA TGAAGAATAT GGTCTTCGAT TGGCCAGCCA TATATTTGTG
    AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT
    GTATTGAAGA TAAATGGTAC CGTGACGGAA AACATGTCAC TGACAGATGC GAAGACGCTG
    ATCGAGAGGT CTAAGGGCAA GTTAAAGATG GTGGTTCAGA GAGATGAGCG GGCTACCCTG
    CTGAATGTCC CTGACCTTTC TGACAGCATC CACTCTGCTG ACGCCTCCGA GAGGGACGAT
    ATTTCAGAAA TCCAGTCACT GGCATCAGAC CATTCAGGTC GATCCCACGA CCGGCCTCCC
    CGTCACAGCC GCTCTCGGTC TCCTGACCAG CGGTCCGAGC CTTCTGACCA TTCTAGACAC
    TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA
    CCAGGGGCTG TCTCGACTCC GGTAAAGCAT GCAGATGATC ATACACCTAA AACAGTGGAA
    GAAGTTGTAG TTGAGCGAAA TGAGAAACAA GCACCCAATC TTCCAGAACC AAAGCCTGTG
    TACGCACAGG TAGGACAGCC AGACGTGGAT TTACCTGTCA GTCCATCTGA TGGCGTCCTG
    CCCAACTCAA CTCATGAAGA TGGCATTCTT CGGCCCAGCA TGAAGTTAGT AAAATTCAGG
    AAAGGAGATA GTGTGGGTCT TCGACTAGCT GGTGGAAACG ATGTTGGCAT ATTTGTAGCT
    GGTGTTCTCG AAGATAGCCC TGCTGCCAAA GAAGGCTTGG AGGAGGGCGA CCAGATCCTC
    AGGGTAAACA ATGTAGATTT CACAAATATC ATAAGAGAAG AAGCCGTCCT CTTCCTGCTT
    GACCTCCCAA AAGGAGAAGA AGTGACCATA TTGGCTCAGA AGAAGAAAGA TGTTTATCGT
    CGCATCGTAG AATCCGATGT GGGTGATTCT TTCTATATCA GAACCCATTT TGAATATGAG
    AAGGAATCTC CCTATGGACT CAGTTTTAAC AAAGGAGAGG TGTTCCGTGT CGTGGACACT
    CTGTACAATG GGAAGCTGGG GTCCTGGCTT GCCATTCGGA TTGGCAAAAA CCATAAAGAG
    GTGGAACGTG GCATCATCCC AAACAAAAAC AGAGCTGAGC AGTTAGCCAG TGTGCAGTAT
    ACACTTCCCA AGACAGCAGG GGGAGACCGC GCCGACTTCT GGAGGTTCCG CGGTCTGCGC
    AGCTCCAAGA GAAACCTGCG CAAGAGCCGA GAAGACCTCT CAGCCCAGCC AGTCCAGACC
    AAGTTCCCGG CGTATGAGAG GGTGGTTCTT CGAGAAGCTG GATTCCTGAG GCCTGTAACC
    ATCTTTGGAC CAATAGCTGA TGTTGCCAGG GAGAAACTGG CAAGAGAGGA GCCAGATATT
    TACCAGATCG CAAAAAGTGA ACCTCGGGAT GCAGGAACCG ACCAGCGCAG CTCTGGCATT
    ATTCGCCTCC ACACAATAAA GCAGATAATA GATCAGGACA AACATGCCTT ACTGGATGTA
    ACGCCCAATG CAGTAGACCG TCTGAACTAT GCCCAGTGGT ATCCAATCGT GGTGTTCCTT
    AACCCGGACT CAAAGCAGGG TGTGAAAACA ATGCGAATGA GGTTATGTCC AGAATCTCGG
    AAAAGCGCCA GGAAGTTATA TGAGCGCTCT CATAAACTTC GTAAAAATAA TCACCATCTT
    TTTACAACTA CAATTAACTT AAATTCAATG AATGATGGCT GGTATGGTGC ACTGAAAGAA
    GCAATCCAAC AACAACAGAA CCAGTTGGTA TGGGTTTCTG AGGGAAAGGC GGATGGTGCT
    ACAAGTGATG ACCTTGATTT GCATGATGAT CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT
    GAATACTCAA TGTATAGCAC GGACAGTAGA CACACTTCTG ACTATGAAGA CACAGACACA
    GAAGGCGGGG CCTACACTGA TCAAGAACTA GATGAGACCC TAAATGATGA GGTGGGGACT
    CCCCCGGAGT CTGCCATTAC CCGGTCCTCT GAGCCTGTCA GAGAGGACTC CTCTGGAATG
    CATCCTGACC ACCAGGCATA TCCTCCCTAC TCACCACAAG CGCAGCCACA GCCAGGCCAT
    AGAATAGACT CCCCTGGATT TAAAACAGCC TCTCAACAGA AAGCAGAAGC CTCATCTCCA
    GGCCCTTACC TTTCGCCTGA AACAAACCCG GCACCATCAG CCTCTGCTGT TAATCCTCAC
    GTAGACTTAA CTAATGTCAG ACTGGAGGAG CCGAGCCCAG CTCCCCCCTC CTCTGACTCA
    CCACACGCCG ACTCTTTACG AGCTCCAGGC ACTGAGGCCG CTCACGTGCA GCTCAAAGGT
    CCAGAGCCGT CCCTGTCGTC GCATGTGGGC CCAGCAAAGG TGTACAGGAA GGACCCCTAC
    CCTGAGGAGA TGATGAGACA GAACCACGTC TTGAAGCAGC CGGCTGTGGT TCACCCTGGG
    CAGAGGCCAG ACAAGGAGCA AGGCCTGAGC TACGAGCCGC AGCCTCCGTA CACAGAGCGA
    CAGGCTGGCA GGGAGTTGGA GCGGCCTGCA TACAGATACG ATGCCTCTAG CTACCCGGAG
    CAGTTTGCTC GGAACTATGA CCATCGTCTG CGCTATGACG ACCGCCTCCT CACCTATGAG
    GAACAATGGC CCTACTACGA TGACAAGCAG CCTTACCAGC CCCGGCCTCT TGATGGTCAG
    CCTCCCGCGG AGCCAACAGA ACGAGGGTAT TTCCCCCGTT TTGAGGAGCC AGTGCCCCTG
    TCCTATGAAG GCCGAGCGCA CTACGACCCA CTGCCCAGGA CCTCCACCTT GCGGCACGAA
    GAGCCTCCCG CCCCTGCCCC TGGGTACGAC GTGCACGGAA GGTACCGGCC TGACGTGCAG
    CCCTACTCCA CTGCTGGCCC CAAGGTGCCC CCTGAGCCCA AGCAGTACTT GGACCAGTAC
    CCTCGGAGTT ACGAGCCAGC GCCAGCCCAG GGCTTCCCCT CCAGGGCAGG CCCAGGCCAC
    TACGAGCCTC TTCACAGCAC CACGCTCGCT CCACCACTTG GCCCCTCGTC CCAGCACAAG
    CCCGAAGTCC CGCCCACAGG CACCAAACCC CTGCCTCCAC CCCCAGCTCC GGCCGAGGAG
    GAGGAAGACC CAGCCATGAA GCCACAATCC GTGCTCACCA GAGTGAAGAT GTTTGAGAAT
    AAGAGGTCTG CTTCCATGGA GAACAGGAAG GATGAGAGCC ATTCCACTAG TTTCAAGCCC
    CCAGAAGTCA CGTCAAAACC GGCAGGTGCT CCTGTCCCAG GTCCCAAAGC CCCTGCTCCG
    AGTCAGCTCG GTGAACACGA CAGAGCAGCA CATAGGGGGA AGGCTGCTGA GGCCGAAGCT
    GTGGACAGGT CATTTGGGGA GAAGCGCTAT GACCCGCTGC AGGCGACGCC CCCCCCTCCG
    CCGCTGCCTG TTCAGTACAG CCAGGGTGCT CAACCCAGCC CCAACACGGC TCTTGGCCTC
    CACGCACACA CCAAGGGGGC GCATGGTGAA GGTAATGCCA TGTCGTTGGA TTTTCAGAAC
    TCGTTAGTGT CCAAACCGGA CCCACCTCCA TCTCAGAATA AGCCAGCAGC TTACAGACCA
    GCCCCTCGAG AGGATGCTGT CCAGTCTGGC TTCTACCCAC AAAAGAGTTT CCCAGAGAAA
    GCCCCAGCTA ATGGGGTCGA GCAGACGCAG AAAACCGTCA CGCCAGCATA CAATCGATTC
    ACACCAAAAC CATACACAAG CTCTGCACGG CCATTTGAAC GCAAGTTTGA AAGCCCTAAA
    TTCAACCACA ATCTCTTGCC GAATGAAACC GCACACAAAC CTGACCTGTC CTCCAAAGCC
    CCCGCTTCTC CGAAAACCCT CCTGAAGTCT GCACAGCCTC CTGAGTTTGA CAGTGGCGTG
    GAGACCCTCT CTGTCCACAC TGACAACAAG CTGAAATACC AAGTAAACAG TGTTAGCATG
    GGGCCGAGAG CAGTGCCCGT GAGTCCCTCA GCCGTGGAGG AAGATGAAGA CGAAGACGGT
    CACACAGTGG TGGCTACTGC CCGCGGCGTG TTTAACAGCA ATGGTGGGGT GCTGAGCTCC
    ATTGAGACCG GTGTCAGCAT CATCATCCCC CAGGGAGCCA TTCCCGAGGG GGTGGAACAG
    GAGATCTACT TCAAGGTCTG CCGCGACAAC AGCATCCTGC CGCCTTTGGA CAAAGAGAAG
    GGTGAGACCC TGCTGAGCCC CCTAGTGATG TGTGGGCCCC ATGGCCTCAA GTTCCTGAAG
    CCCGTGGAGC TGCGCCTACC ACACTGTGCG TCCATGACTC CTGACGGTTG GTCTTTTGCT
    CTAAAATCAT CCGACTCCTC GTCGGGTGAT CCTAAAACCT GGCAAAACAA GTGTCTTCCT
    GGAGATCCAA ATTATCTTGT TGGAGCAAAC TGTGTTTCTG TCCTGATTGA CCACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837773.1
    CDS50..5389
    Translation

    Target ORF information:

    RefSeq Version XM_024982005.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 1 (TJP1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982005.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGGGCCC TGGCCGCGAG 
    CGGAGCGGGG ACAAGATGAA GTACCAGAAG TACCTGACGG TGCTGCAGAT GGCCATCGGC
    GTCACGCCGT CCAACCGCGG CAGCCTGCTG CCGCTCAAGA GGAGGCTGTG GGTAACGCCG
    TCCTCTGAAA ACCCCAGTGG TGCTACTTCT AGTGTCAGCC AAGGAAAGCC TTCTTTAAGA
    CGAATTAAAG GGAGACTGCA CAGGAGCAAG AGCCTCGATA GCATCGATTT CTGTGAACTC
    ACTAACACAA CAATGGAGGA AACAGCTATA TGGGAACAAC ACACGGTGAC ACTTCACAGG
    GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC ACATTTCCAG
    AGCGGGGAAA CCTCCATCGT GATTTCAGAT GTGCTGAAGG GGGGGCCTGC GGAAGGACAG
    CTGCAGGAAA ATGACCGGGT GGCAATGGTT AATGGAGTCT CCATGGATAA TGTTGAGCAT
    GCGTTTGCTG TTCAGCAACT AAGGAAAAGT GGGAAAAATG CAAAAATAAC CATTCGAAGG
    AAGAAGAAAG TTCAGATCCC AGTAAATCGT CCTGATCCTG AGCCTGTGTC TGAGAACGAA
    GAGGACAGTT ACGACGAGGA AGCGCATGAC CCGAGAAGTG GCCGTGGGGC CCTGGCAAAC
    AGGAGGAGCG AGAAGAGCTG GGGCCGAGAT AGGAGTGCCA GTCGGGAGAG GAGTCTGTCC
    CCGCGGTCTG ACAGGCGGTC TGTAGCCTCC AGTCAGCCTG CTAGACCCAC CAAAGTCACC
    CTGGTGAAGT CCCGGAAGAA TGAAGAATAT GGTCTTCGAT TGGCCAGCCA TATATTTGTG
    AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT
    GTATTGAAGA TAAATGGTAC CGTGACGGAA AACATGTCAC TGACAGATGC GAAGACGCTG
    ATCGAGAGGT CTAAGGGCAA GTTAAAGATG GTGGTTCAGA GAGATGAGCG GGCTACCCTG
    CTGAATGTCC CTGACCTTTC TGACAGCATC CACTCTGCTG ACGCCTCCGA GAGGGACGAT
    ATTTCAGAAA TCCAGTCACT GGCATCAGAC CATTCAGGTC GATCCCACGA CCGGCCTCCC
    CGTCACAGCC GCTCTCGGTC TCCTGACCAG CGGTCCGAGC CTTCTGACCA TTCTAGACAC
    TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA
    CCAGGGGCTG TCTCGACTCC GGTAAAGCAT GCAGATGATC ATACACCTAA AACAGTGGAA
    GAAGTTGTAG TTGAGCGAAA TGAGAAACAA GCACCCAATC TTCCAGAACC AAAGCCTGTG
    TACGCACAGG TAGGACAGCC AGACGTGGAT TTACCTGTCA GTCCATCTGA TGGCGTCCTG
    CCCAACTCAA CTCATGAAGA TGGCATTCTT CGGCCCAGCA TGAAGTTAGT AAAATTCAGG
    AAAGGAGATA GTGTGGGTCT TCGACTAGCT GGTGGAAACG ATGTTGGCAT ATTTGTAGCT
    GGTGTTCTCG AAGATAGCCC TGCTGCCAAA GAAGGCTTGG AGGAGGGCGA CCAGATCCTC
    AGGGTAAACA ATGTAGATTT CACAAATATC ATAAGAGAAG AAGCCGTCCT CTTCCTGCTT
    GACCTCCCAA AAGGAGAAGA AGTGACCATA TTGGCTCAGA AGAAGAAAGA TGTTTATCGT
    CGCATCGTAG AATCCGATGT GGGTGATTCT TTCTATATCA GAACCCATTT TGAATATGAG
    AAGGAATCTC CCTATGGACT CAGTTTTAAC AAAGGAGAGG TGTTCCGTGT CGTGGACACT
    CTGTACAATG GGAAGCTGGG GTCCTGGCTT GCCATTCGGA TTGGCAAAAA CCATAAAGAG
    GTGGAACGTG GCATCATCCC AAACAAAAAC AGAGCTGAGC AGTTAGCCAG TGTGCAGTAT
    ACACTTCCCA AGACAGCAGG GGGAGACCGC GCCGACTTCT GGAGGTTCCG CGGTCTGCGC
    AGCTCCAAGA GAAACCTGCG CAAGAGCCGA GAAGACCTCT CAGCCCAGCC AGTCCAGACC
    AAGTTCCCGG CGTATGAGAG GGTGGTTCTT CGAGAAGCTG GATTCCTGAG GCCTGTAACC
    ATCTTTGGAC CAATAGCTGA TGTTGCCAGG GAGAAACTGG CAAGAGAGGA GCCAGATATT
    TACCAGATCG CAAAAAGTGA ACCTCGGGAT GCAGGAACCG ACCAGCGCAG CTCTGGCATT
    ATTCGCCTCC ACACAATAAA GCAGATAATA GATCAGGACA AACATGCCTT ACTGGATGTA
    ACGCCCAATG CAGTAGACCG TCTGAACTAT GCCCAGTGGT ATCCAATCGT GGTGTTCCTT
    AACCCGGACT CAAAGCAGGG TGTGAAAACA ATGCGAATGA GGTTATGTCC AGAATCTCGG
    AAAAGCGCCA GGAAGTTATA TGAGCGCTCT CATAAACTTC GTAAAAATAA TCACCATCTT
    TTTACAACTA CAATTAACTT AAATTCAATG AATGATGGCT GGTATGGTGC ACTGAAAGAA
    GCAATCCAAC AACAACAGAA CCAGTTGGTA TGGGTTTCTG AGGGAAAGGC GGATGGTGCT
    ACAAGTGATG ACCTTGATTT GCATGATGAT CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT
    GAATACTCAA TGTATAGCAC GGACAGTAGA CACACTTCTG ACTATGAAGA CACAGACACA
    GAAGGCGGGG CCTACACTGA TCAAGAACTA GATGAGACCC TAAATGATGA GGTGGGGACT
    CCCCCGGAGT CTGCCATTAC CCGGTCCTCT GAGCCTGTCA GAGAGGACTC CTCTGGAATG
    CATCCTGACC ACCAGGCATA TCCTCCCTAC TCACCACAAG CGCAGCCACA GCCAGGCCAT
    AGAATAGACT CCCCTGGATT TAAAACAGCC TCTCAACAGA AAGCAGAAGC CTCATCTCCA
    GGCCCTTACC TTTCGCCTGA AACAAACCCG GCACCATCAG CCTCTGCTGT TAATCCTCAC
    GTAGACTTAA CTAATGTCAG ACTGGAGGAG CCGAGCCCAG CTCCCCCCTC CTCTGACTCA
    CCACACGCCG ACTCTTTACG AGCTCCAGGC ACTGAGGCCG CTCACGTGCA GCTCAAAGGT
    CCAGAGCCGT CCCTGTCGTC GCATGTGGGC CCAGCAAAGG TGTACAGGAA GGACCCCTAC
    CCTGAGGAGA TGATGAGACA GAACCACGTC TTGAAGCAGC CGGCTGTGGT TCACCCTGGG
    CAGAGGCCAG ACAAGGAGCA AGGCCTGAGC TACGAGCCGC AGCCTCCGTA CACAGAGCGA
    CAGGCTGGCA GGGAGTTGGA GCGGCCTGCA TACAGATACG ATGCCTCTAG CTACCCGGAG
    CAGTTTGCTC GGAACTATGA CCATCGTCTG CGCTATGACG ACCGCCTCCT CACCTATGAG
    GAACAATGGC CCTACTACGA TGACAAGCAG CCTTACCAGC CCCGGCCTCT TGATGGTCAG
    CCTCCCGCGG AGCCAACAGA ACGAGGGTAT TTCCCCCGTT TTGAGGAGCC AGTGCCCCTG
    TCCTATGAAG GCCGAGCGCA CTACGACCCA CTGCCCAGGA CCTCCACCTT GCGGCACGAA
    GAGCCTCCCG CCCCTGCCCC TGGGTACGAC GTGCACGGAA GGTACCGGCC TGACGTGCAG
    CCCTACTCCA CTGCTGGCCC CAAGGTGCCC CCTGAGCCCA AGCAGTACTT GGACCAGTAC
    CCTCGGAGTT ACGAGCCAGC GCCAGCCCAG GGCTTCCCCT CCAGGGCAGG CCCAGGCCAC
    TACGAGCCTC TTCACAGCAC CACGCTCGCT CCACCACTTG GCCCCTCGTC CCAGCACAAG
    CCCGAAGTCC CGCCCACAGG CACCAAACCC CTGCCTCCAC CCCCAGCTCC GGCCGAGGAG
    GAGGAAGACC CAGCCATGAA GCCACAATCC GTGCTCACCA GAGTGAAGAT GTTTGAGAAT
    AAGAGGTCTG CTTCCATGGA GAACAGGAAG GATGAGAGCC ATTCCACTAG TTTCAAGCCC
    CCAGAAGTCA CGTCAAAACC GGCAGGTGCT CCTGTCCCAG GTCCCAAAGC CCCTGCTCCG
    AGTCAGCTCG GTGAACACGA CAGAGCAGCA CATAGGGGGA AGGCTGCTGA GGCCGAAGCT
    GTGGACAGGT CATTTGGGGA GAAGCGCTAT GACCCGCTGC AGGCGACGCC CCCCCCTCCG
    CCGCTGCCTG TTCAGTACAG CCAGGGTGCT CAACCCAGCC CCAACACGGC TCTTGGCCTC
    CACGCACACA CCAAGGGGGC GCATGGTGAA GGTAATGCCA TGTCGTTGGA TTTTCAGAAC
    TCGTTAGTGT CCAAACCGGA CCCACCTCCA TCTCAGAATA AGCCAGCAGC TTACAGACCA
    GCCCCTCGAG AGGATGCTGT CCAGTCTGGC TTCTACCCAC AAAAGAGTTT CCCAGAGAAA
    GCCCCAGCTA ATGGGGTCGA GCAGACGCAG AAAACCGTCA CGCCAGCATA CAATCGATTC
    ACACCAAAAC CATACACAAG CTCTGCACGG CCATTTGAAC GCAAGTTTGA AAGCCCTAAA
    TTCAACCACA ATCTCTTGCC GAATGAAACC GCACACAAAC CTGACCTGTC CTCCAAAGCC
    CCCGCTTCTC CGAAAACCCT CCTGAAGTCT GCACAGCCTC CTGAGTTTGA CAGTGGCGTG
    GAGACCCTCT CTGTCCACAC TGACAACAAG CTGAAATACC AAGTAAACAG TGTTAGCATG
    GGGCCGAGAG CAGTGCCCGT GAGTCCCTCA GCCGTGGAGG AAGATGAAGA CGAAGACGGT
    CACACAGTGG TGGCTACTGC CCGCGGCGTG TTTAACAGCA ATGGTGGGGT GCTGAGCTCC
    ATTGAGACCG GTGTCAGCAT CATCATCCCC CAGGGAGCCA TTCCCGAGGG GGTGGAACAG
    GAGATCTACT TCAAGGTCTG CCGCGACAAC AGCATCCTGC CGCCTTTGGA CAAAGAGAAG
    GGTGAGACCC TGCTGAGCCC CCTAGTGATG TGTGGGCCCC ATGGCCTCAA GTTCCTGAAG
    CCCGTGGAGC TGCGCCTACC ACACTGTGCG TCCATGACTC CTGACGGTTG GTCTTTTGCT
    CTAAAATCAT CCGACTCCTC GTCGGGTGAT CCTAAAACCT GGCAAAACAA GTGTCTTCCT
    GGAGATCCAA ATTATCTTGT TGGAGCAAAC TGTGTTTCTG TCCTGATTGA CCACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228435
    Clone ID Related Accession (Same CDS sequence) XM_024982006.1
    Accession Version XM_024982006.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGGGCCC TGGCCGCGAG 
    CGGAGCGGGG ACAAGATGAA GTACCAGAAG TACCTGACGG TGCTGCAGAT GGCCATCGGC
    GTCACGCCGT CCAACCGCGG CAGCCTGCTG CCGCTCAAGA GGAGGCTGTG GGTAACGCCG
    TCCTCTGAAA ACCCCAGTGG TGCTACTTCT AGTGTCAGCC AAGGAAAGCC TTCTTTAAGA
    CGAATTAAAG GGAGACTGCA CAGGAGCAAG AGCCTCGATA GCATCGATTT CTGTGAACTC
    ACTAACACAA CAATGGAGGA AACAGCTATA TGGGAACAAC ACACGGTGAC ACTTCACAGG
    GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC ACATTTCCAG
    AGCGGGGAAA CCTCCATCGT GATTTCAGAT GTGCTGAAGG GGGGGCCTGC GGAAGGACAG
    CTGCAGGAAA ATGACCGGGT GGCAATGGTT AATGGAGTCT CCATGGATAA TGTTGAGCAT
    GCGTTTGCTG TTCAGCAACT AAGGAAAAGT GGGAAAAATG CAAAAATAAC CATTCGAAGG
    AAGAAGAAAG TTCAGATCCC AGTAAATCGT CCTGATCCTG AGCCTGTGTC TGAGAACGAA
    GAGGACAGTT ACGACGAGGA AGCGCATGAC CCGAGAAGTG GCCGTGGGGC CCTGGCAAAC
    AGGAGGAGCG AGAAGAGCTG GGGCCGAGAT AGGAGTGCCA GTCGGGAGAG GAGTCTGTCC
    CCGCGGTCTG ACAGGCGGTC TGTAGCCTCC AGTCAGCCTG CTAGACCCAC CAAAGTCACC
    CTGGTGAAGT CCCGGAAGAA TGAAGAATAT GGTCTTCGAT TGGCCAGCCA TATATTTGTG
    AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT
    GTATTGAAGA TAAATGGTAC CGTGACGGAA AACATGTCAC TGACAGATGC GAAGACGCTG
    ATCGAGAGGT CTAAGGGCAA GTTAAAGATG GTGGTTCAGA GAGATGAGCG GGCTACCCTG
    CTGAATGTCC CTGACCTTTC TGACAGCATC CACTCTGCTG ACGCCTCCGA GAGGGACGAT
    ATTTCAGAAA TCCAGTCACT GGCATCAGAC CATTCAGGTC GATCCCACGA CCGGCCTCCC
    CGTCACAGCC GCTCTCGGTC TCCTGACCAG CGGTCCGAGC CTTCTGACCA TTCTAGACAC
    TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA
    CCAGGGGCTG TCTCGACTCC GGTAAAGCAT GCAGATGATC ATACACCTAA AACAGTGGAA
    GAAGTTGTAG TTGAGCGAAA TGAGAAACAA GCACCCAATC TTCCAGAACC AAAGCCTGTG
    TACGCACAGG TAGGACAGCC AGACGTGGAT TTACCTGTCA GTCCATCTGA TGGCGTCCTG
    CCCAACTCAA CTCATGAAGA TGGCATTCTT CGGCCCAGCA TGAAGTTAGT AAAATTCAGG
    AAAGGAGATA GTGTGGGTCT TCGACTAGCT GGTGGAAACG ATGTTGGCAT ATTTGTAGCT
    GGTGTTCTCG AAGATAGCCC TGCTGCCAAA GAAGGCTTGG AGGAGGGCGA CCAGATCCTC
    AGGGTAAACA ATGTAGATTT CACAAATATC ATAAGAGAAG AAGCCGTCCT CTTCCTGCTT
    GACCTCCCAA AAGGAGAAGA AGTGACCATA TTGGCTCAGA AGAAGAAAGA TGTTTATCGT
    CGCATCGTAG AATCCGATGT GGGTGATTCT TTCTATATCA GAACCCATTT TGAATATGAG
    AAGGAATCTC CCTATGGACT CAGTTTTAAC AAAGGAGAGG TGTTCCGTGT CGTGGACACT
    CTGTACAATG GGAAGCTGGG GTCCTGGCTT GCCATTCGGA TTGGCAAAAA CCATAAAGAG
    GTGGAACGTG GCATCATCCC AAACAAAAAC AGAGCTGAGC AGTTAGCCAG TGTGCAGTAT
    ACACTTCCCA AGACAGCAGG GGGAGACCGC GCCGACTTCT GGAGGTTCCG CGGTCTGCGC
    AGCTCCAAGA GAAACCTGCG CAAGAGCCGA GAAGACCTCT CAGCCCAGCC AGTCCAGACC
    AAGTTCCCGG CGTATGAGAG GGTGGTTCTT CGAGAAGCTG GATTCCTGAG GCCTGTAACC
    ATCTTTGGAC CAATAGCTGA TGTTGCCAGG GAGAAACTGG CAAGAGAGGA GCCAGATATT
    TACCAGATCG CAAAAAGTGA ACCTCGGGAT GCAGGAACCG ACCAGCGCAG CTCTGGCATT
    ATTCGCCTCC ACACAATAAA GCAGATAATA GATCAGGACA AACATGCCTT ACTGGATGTA
    ACGCCCAATG CAGTAGACCG TCTGAACTAT GCCCAGTGGT ATCCAATCGT GGTGTTCCTT
    AACCCGGACT CAAAGCAGGG TGTGAAAACA ATGCGAATGA GGTTATGTCC AGAATCTCGG
    AAAAGCGCCA GGAAGTTATA TGAGCGCTCT CATAAACTTC GTAAAAATAA TCACCATCTT
    TTTACAACTA CAATTAACTT AAATTCAATG AATGATGGCT GGTATGGTGC ACTGAAAGAA
    GCAATCCAAC AACAACAGAA CCAGTTGGTA TGGGTTTCTG AGGGAAAGGC GGATGGTGCT
    ACAAGTGATG ACCTTGATTT GCATGATGAT CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT
    GAATACTCAA TGTATAGCAC GGACAGTAGA CACACTTCTG ACTATGAAGA CACAGACACA
    GAAGGCGGGG CCTACACTGA TCAAGAACTA GATGAGACCC TAAATGATGA GGTGGGGACT
    CCCCCGGAGT CTGCCATTAC CCGGTCCTCT GAGCCTGTCA GAGAGGACTC CTCTGGAATG
    CATCCTGACC ACCAGGCATA TCCTCCCTAC TCACCACAAG CGCAGCCACA GCCAGGCCAT
    AGAATAGACT CCCCTGGATT TAAAACAGCC TCTCAACAGG TGTACAGGAA GGACCCCTAC
    CCTGAGGAGA TGATGAGACA GAACCACGTC TTGAAGCAGC CGGCTGTGGT TCACCCTGGG
    CAGAGGCCAG ACAAGGAGCA AGGCCTGAGC TACGAGCCGC AGCCTCCGTA CACAGAGCGA
    CAGGCTGGCA GGGAGTTGGA GCGGCCTGCA TACAGATACG ATGCCTCTAG CTACCCGGAG
    CAGTTTGCTC GGAACTATGA CCATCGTCTG CGCTATGACG ACCGCCTCCT CACCTATGAG
    GAACAATGGC CCTACTACGA TGACAAGCAG CCTTACCAGC CCCGGCCTCT TGATGGTCAG
    CCTCCCGCGG AGCCAACAGA ACGAGGGTAT TTCCCCCGTT TTGAGGAGCC AGTGCCCCTG
    TCCTATGAAG GCCGAGCGCA CTACGACCCA CTGCCCAGGA CCTCCACCTT GCGGCACGAA
    GAGCCTCCCG CCCCTGCCCC TGGGTACGAC GTGCACGGAA GGTACCGGCC TGACGTGCAG
    CCCTACTCCA CTGCTGGCCC CAAGGTGCCC CCTGAGCCCA AGCAGTACTT GGACCAGTAC
    CCTCGGAGTT ACGAGCCAGC GCCAGCCCAG GGCTTCCCCT CCAGGGCAGG CCCAGGCCAC
    TACGAGCCTC TTCACAGCAC CACGCTCGCT CCACCACTTG GCCCCTCGTC CCAGCACAAG
    CCCGAAGTCC CGCCCACAGG CACCAAACCC CTGCCTCCAC CCCCAGCTCC GGCCGAGGAG
    GAGGAAGACC CAGCCATGAA GCCACAATCC GTGCTCACCA GAGTGAAGAT GTTTGAGAAT
    AAGAGGTCTG CTTCCATGGA GAACAGGAAG GATGAGAGCC ATTCCACTAG TTTCAAGCCC
    CCAGAAGTCA CGTCAAAACC GGCAGGTGCT CCTGTCCCAG GTCCCAAAGC CCCTGCTCCG
    AGTCAGCTCG GTGAACACGA CAGAGCAGCA CATAGGATCC CTGAACCACA GAGGCCTCAC
    ATGAAGCCAC CTGAAGACAT TGTTCGGGCA AATCACTATG ACCCCGAGGA GGATGAGGAG
    TACTACCGGA AGCAGCTCTC CTACTTCGAT CGGAGAAGCT TTGAGGCCAA GCCCCCCACC
    CACATCCCCA CTAGCCACCT CTCCGAGCCC ACCAGGCCGG CTCACCCCCA ATCTCAGCCA
    CAGTTTGCCG GTTACTCTTC CAAGGGGAAG GCTGCTGAGG CCGAAGCTGT GGACAGGTCA
    TTTGGGGAGA AGCGCTATGA CCCGCTGCAG GCGACGCCCC CCCCTCCGCC GCTGCCTGTT
    CAGTACAGCC AGGGTGCTCA ACCCAGCCCC AACACGGCTC TTGGCCTCCA CGCACACACC
    AAGGGGGCGC ATGGTGAAGG TAATGCCATG TCGTTGGATT TTCAGAACTC GTTAGTGTCC
    AAACCGGACC CACCTCCATC TCAGAATAAG CCAGCAGCTT ACAGACCAGC CCCTCGAGAG
    GATGCTGTCC AGTCTGGCTT CTACCCACAA AAGAGTTTCC CAGAGAAAGC CCCAGCTAAT
    GGGGTCGAGC AGACGCAGAA AACCGTCACG CCAGCATACA ATCGATTCAC ACCAAAACCA
    TACACAAGCT CTGCACGGCC ATTTGAACGC AAGTTTGAAA GCCCTAAATT CAACCACAAT
    CTCTTGCCGA ATGAAACCGC ACACAAACCT GACCTGTCCT CCAAAGCCCC CGCTTCTCCG
    AAAACCCTCC TGAAGTCTGC ACAGCCTCCT GAGTTTGACA GTGGCGTGGA GACCCTCTCT
    GTCCACACTG ACAACAAGCT GAAATACCAA GTAAACAGTG TTAGCATGGG GCCGAGAGCA
    GTGCCCGTGA GTCCCTCAGC CGTGGAGGAA GATGAAGACG AAGACGGTCA CACAGTGGTG
    GCTACTGCCC GCGGCGTGTT TAACAGCAAT GGTGGGGTGC TGAGCTCCAT TGAGACCGGT
    GTCAGCATCA TCATCCCCCA GGGAGCCATT CCCGAGGGGG TGGAACAGGA GATCTACTTC
    AAGGTCTGCC GCGACAACAG CATCCTGCCG CCTTTGGACA AAGAGAAGGG TGAGACCCTG
    CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG
    CGCCTACCAC ACTGTGCGTC CATGACTCCT GACGGTTGGT CTTTTGCTCT AAAATCATCC
    GACTCCTCGT CGGGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCAAAT
    TATCTTGTTG GAGCAAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837774.1
    CDS50..5377
    Translation

    Target ORF information:

    RefSeq Version XM_024982006.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 1 (TJP1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982006.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGGGCCC TGGCCGCGAG 
    CGGAGCGGGG ACAAGATGAA GTACCAGAAG TACCTGACGG TGCTGCAGAT GGCCATCGGC
    GTCACGCCGT CCAACCGCGG CAGCCTGCTG CCGCTCAAGA GGAGGCTGTG GGTAACGCCG
    TCCTCTGAAA ACCCCAGTGG TGCTACTTCT AGTGTCAGCC AAGGAAAGCC TTCTTTAAGA
    CGAATTAAAG GGAGACTGCA CAGGAGCAAG AGCCTCGATA GCATCGATTT CTGTGAACTC
    ACTAACACAA CAATGGAGGA AACAGCTATA TGGGAACAAC ACACGGTGAC ACTTCACAGG
    GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC ACATTTCCAG
    AGCGGGGAAA CCTCCATCGT GATTTCAGAT GTGCTGAAGG GGGGGCCTGC GGAAGGACAG
    CTGCAGGAAA ATGACCGGGT GGCAATGGTT AATGGAGTCT CCATGGATAA TGTTGAGCAT
    GCGTTTGCTG TTCAGCAACT AAGGAAAAGT GGGAAAAATG CAAAAATAAC CATTCGAAGG
    AAGAAGAAAG TTCAGATCCC AGTAAATCGT CCTGATCCTG AGCCTGTGTC TGAGAACGAA
    GAGGACAGTT ACGACGAGGA AGCGCATGAC CCGAGAAGTG GCCGTGGGGC CCTGGCAAAC
    AGGAGGAGCG AGAAGAGCTG GGGCCGAGAT AGGAGTGCCA GTCGGGAGAG GAGTCTGTCC
    CCGCGGTCTG ACAGGCGGTC TGTAGCCTCC AGTCAGCCTG CTAGACCCAC CAAAGTCACC
    CTGGTGAAGT CCCGGAAGAA TGAAGAATAT GGTCTTCGAT TGGCCAGCCA TATATTTGTG
    AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT
    GTATTGAAGA TAAATGGTAC CGTGACGGAA AACATGTCAC TGACAGATGC GAAGACGCTG
    ATCGAGAGGT CTAAGGGCAA GTTAAAGATG GTGGTTCAGA GAGATGAGCG GGCTACCCTG
    CTGAATGTCC CTGACCTTTC TGACAGCATC CACTCTGCTG ACGCCTCCGA GAGGGACGAT
    ATTTCAGAAA TCCAGTCACT GGCATCAGAC CATTCAGGTC GATCCCACGA CCGGCCTCCC
    CGTCACAGCC GCTCTCGGTC TCCTGACCAG CGGTCCGAGC CTTCTGACCA TTCTAGACAC
    TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA
    CCAGGGGCTG TCTCGACTCC GGTAAAGCAT GCAGATGATC ATACACCTAA AACAGTGGAA
    GAAGTTGTAG TTGAGCGAAA TGAGAAACAA GCACCCAATC TTCCAGAACC AAAGCCTGTG
    TACGCACAGG TAGGACAGCC AGACGTGGAT TTACCTGTCA GTCCATCTGA TGGCGTCCTG
    CCCAACTCAA CTCATGAAGA TGGCATTCTT CGGCCCAGCA TGAAGTTAGT AAAATTCAGG
    AAAGGAGATA GTGTGGGTCT TCGACTAGCT GGTGGAAACG ATGTTGGCAT ATTTGTAGCT
    GGTGTTCTCG AAGATAGCCC TGCTGCCAAA GAAGGCTTGG AGGAGGGCGA CCAGATCCTC
    AGGGTAAACA ATGTAGATTT CACAAATATC ATAAGAGAAG AAGCCGTCCT CTTCCTGCTT
    GACCTCCCAA AAGGAGAAGA AGTGACCATA TTGGCTCAGA AGAAGAAAGA TGTTTATCGT
    CGCATCGTAG AATCCGATGT GGGTGATTCT TTCTATATCA GAACCCATTT TGAATATGAG
    AAGGAATCTC CCTATGGACT CAGTTTTAAC AAAGGAGAGG TGTTCCGTGT CGTGGACACT
    CTGTACAATG GGAAGCTGGG GTCCTGGCTT GCCATTCGGA TTGGCAAAAA CCATAAAGAG
    GTGGAACGTG GCATCATCCC AAACAAAAAC AGAGCTGAGC AGTTAGCCAG TGTGCAGTAT
    ACACTTCCCA AGACAGCAGG GGGAGACCGC GCCGACTTCT GGAGGTTCCG CGGTCTGCGC
    AGCTCCAAGA GAAACCTGCG CAAGAGCCGA GAAGACCTCT CAGCCCAGCC AGTCCAGACC
    AAGTTCCCGG CGTATGAGAG GGTGGTTCTT CGAGAAGCTG GATTCCTGAG GCCTGTAACC
    ATCTTTGGAC CAATAGCTGA TGTTGCCAGG GAGAAACTGG CAAGAGAGGA GCCAGATATT
    TACCAGATCG CAAAAAGTGA ACCTCGGGAT GCAGGAACCG ACCAGCGCAG CTCTGGCATT
    ATTCGCCTCC ACACAATAAA GCAGATAATA GATCAGGACA AACATGCCTT ACTGGATGTA
    ACGCCCAATG CAGTAGACCG TCTGAACTAT GCCCAGTGGT ATCCAATCGT GGTGTTCCTT
    AACCCGGACT CAAAGCAGGG TGTGAAAACA ATGCGAATGA GGTTATGTCC AGAATCTCGG
    AAAAGCGCCA GGAAGTTATA TGAGCGCTCT CATAAACTTC GTAAAAATAA TCACCATCTT
    TTTACAACTA CAATTAACTT AAATTCAATG AATGATGGCT GGTATGGTGC ACTGAAAGAA
    GCAATCCAAC AACAACAGAA CCAGTTGGTA TGGGTTTCTG AGGGAAAGGC GGATGGTGCT
    ACAAGTGATG ACCTTGATTT GCATGATGAT CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT
    GAATACTCAA TGTATAGCAC GGACAGTAGA CACACTTCTG ACTATGAAGA CACAGACACA
    GAAGGCGGGG CCTACACTGA TCAAGAACTA GATGAGACCC TAAATGATGA GGTGGGGACT
    CCCCCGGAGT CTGCCATTAC CCGGTCCTCT GAGCCTGTCA GAGAGGACTC CTCTGGAATG
    CATCCTGACC ACCAGGCATA TCCTCCCTAC TCACCACAAG CGCAGCCACA GCCAGGCCAT
    AGAATAGACT CCCCTGGATT TAAAACAGCC TCTCAACAGG TGTACAGGAA GGACCCCTAC
    CCTGAGGAGA TGATGAGACA GAACCACGTC TTGAAGCAGC CGGCTGTGGT TCACCCTGGG
    CAGAGGCCAG ACAAGGAGCA AGGCCTGAGC TACGAGCCGC AGCCTCCGTA CACAGAGCGA
    CAGGCTGGCA GGGAGTTGGA GCGGCCTGCA TACAGATACG ATGCCTCTAG CTACCCGGAG
    CAGTTTGCTC GGAACTATGA CCATCGTCTG CGCTATGACG ACCGCCTCCT CACCTATGAG
    GAACAATGGC CCTACTACGA TGACAAGCAG CCTTACCAGC CCCGGCCTCT TGATGGTCAG
    CCTCCCGCGG AGCCAACAGA ACGAGGGTAT TTCCCCCGTT TTGAGGAGCC AGTGCCCCTG
    TCCTATGAAG GCCGAGCGCA CTACGACCCA CTGCCCAGGA CCTCCACCTT GCGGCACGAA
    GAGCCTCCCG CCCCTGCCCC TGGGTACGAC GTGCACGGAA GGTACCGGCC TGACGTGCAG
    CCCTACTCCA CTGCTGGCCC CAAGGTGCCC CCTGAGCCCA AGCAGTACTT GGACCAGTAC
    CCTCGGAGTT ACGAGCCAGC GCCAGCCCAG GGCTTCCCCT CCAGGGCAGG CCCAGGCCAC
    TACGAGCCTC TTCACAGCAC CACGCTCGCT CCACCACTTG GCCCCTCGTC CCAGCACAAG
    CCCGAAGTCC CGCCCACAGG CACCAAACCC CTGCCTCCAC CCCCAGCTCC GGCCGAGGAG
    GAGGAAGACC CAGCCATGAA GCCACAATCC GTGCTCACCA GAGTGAAGAT GTTTGAGAAT
    AAGAGGTCTG CTTCCATGGA GAACAGGAAG GATGAGAGCC ATTCCACTAG TTTCAAGCCC
    CCAGAAGTCA CGTCAAAACC GGCAGGTGCT CCTGTCCCAG GTCCCAAAGC CCCTGCTCCG
    AGTCAGCTCG GTGAACACGA CAGAGCAGCA CATAGGATCC CTGAACCACA GAGGCCTCAC
    ATGAAGCCAC CTGAAGACAT TGTTCGGGCA AATCACTATG ACCCCGAGGA GGATGAGGAG
    TACTACCGGA AGCAGCTCTC CTACTTCGAT CGGAGAAGCT TTGAGGCCAA GCCCCCCACC
    CACATCCCCA CTAGCCACCT CTCCGAGCCC ACCAGGCCGG CTCACCCCCA ATCTCAGCCA
    CAGTTTGCCG GTTACTCTTC CAAGGGGAAG GCTGCTGAGG CCGAAGCTGT GGACAGGTCA
    TTTGGGGAGA AGCGCTATGA CCCGCTGCAG GCGACGCCCC CCCCTCCGCC GCTGCCTGTT
    CAGTACAGCC AGGGTGCTCA ACCCAGCCCC AACACGGCTC TTGGCCTCCA CGCACACACC
    AAGGGGGCGC ATGGTGAAGG TAATGCCATG TCGTTGGATT TTCAGAACTC GTTAGTGTCC
    AAACCGGACC CACCTCCATC TCAGAATAAG CCAGCAGCTT ACAGACCAGC CCCTCGAGAG
    GATGCTGTCC AGTCTGGCTT CTACCCACAA AAGAGTTTCC CAGAGAAAGC CCCAGCTAAT
    GGGGTCGAGC AGACGCAGAA AACCGTCACG CCAGCATACA ATCGATTCAC ACCAAAACCA
    TACACAAGCT CTGCACGGCC ATTTGAACGC AAGTTTGAAA GCCCTAAATT CAACCACAAT
    CTCTTGCCGA ATGAAACCGC ACACAAACCT GACCTGTCCT CCAAAGCCCC CGCTTCTCCG
    AAAACCCTCC TGAAGTCTGC ACAGCCTCCT GAGTTTGACA GTGGCGTGGA GACCCTCTCT
    GTCCACACTG ACAACAAGCT GAAATACCAA GTAAACAGTG TTAGCATGGG GCCGAGAGCA
    GTGCCCGTGA GTCCCTCAGC CGTGGAGGAA GATGAAGACG AAGACGGTCA CACAGTGGTG
    GCTACTGCCC GCGGCGTGTT TAACAGCAAT GGTGGGGTGC TGAGCTCCAT TGAGACCGGT
    GTCAGCATCA TCATCCCCCA GGGAGCCATT CCCGAGGGGG TGGAACAGGA GATCTACTTC
    AAGGTCTGCC GCGACAACAG CATCCTGCCG CCTTTGGACA AAGAGAAGGG TGAGACCCTG
    CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG
    CGCCTACCAC ACTGTGCGTC CATGACTCCT GACGGTTGGT CTTTTGCTCT AAAATCATCC
    GACTCCTCGT CGGGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCAAAT
    TATCTTGTTG GAGCAAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228436
    Clone ID Related Accession (Same CDS sequence) XM_024982007.1
    Accession Version XM_024982007.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5292bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCCGCCA GGGCCGCGGC CGCCAAGAAC ACAACAATGG AGGAAACAGC TATATGGGAA 
    CAACACACGG TGACACTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGAAGAGATA ATCCACATTT CCAGAGCGGG GAAACCTCCA TCGTGATTTC AGATGTGCTG
    AAGGGGGGGC CTGCGGAAGG ACAGCTGCAG GAAAATGACC GGGTGGCAAT GGTTAATGGA
    GTCTCCATGG ATAATGTTGA GCATGCGTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TAACCATTCG AAGGAAGAAG AAAGTTCAGA TCCCAGTAAA TCGTCCTGAT
    CCTGAGCCTG TGTCTGAGAA CGAAGAGGAC AGTTACGACG AGGAAGCGCA TGACCCGAGA
    AGTGGCCGTG GGGCCCTGGC AAACAGGAGG AGCGAGAAGA GCTGGGGCCG AGATAGGAGT
    GCCAGTCGGG AGAGGAGTCT GTCCCCGCGG TCTGACAGGC GGTCTGTAGC CTCCAGTCAG
    CCTGCTAGAC CCACCAAAGT CACCCTGGTG AAGTCCCGGA AGAATGAAGA ATATGGTCTT
    CGATTGGCCA GCCATATATT TGTGAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGCGA TGTTGTATTG AAGATAAATG GTACCGTGAC GGAAAACATG
    TCACTGACAG ATGCGAAGAC GCTGATCGAG AGGTCTAAGG GCAAGTTAAA GATGGTGGTT
    CAGAGAGATG AGCGGGCTAC CCTGCTGAAT GTCCCTGACC TTTCTGACAG CATCCACTCT
    GCTGACGCCT CCGAGAGGGA CGATATTTCA GAAATCCAGT CACTGGCATC AGACCATTCA
    GGTCGATCCC ACGACCGGCC TCCCCGTCAC AGCCGCTCTC GGTCTCCTGA CCAGCGGTCC
    GAGCCTTCTG ACCATTCTAG ACACTCTCCA CAGCAGCCCA GCAGTGGCAG TCTCCGGAGC
    AGAGAGGAAG AGAGAATTTC TAAACCAGGG GCTGTCTCGA CTCCGGTAAA GCATGCAGAT
    GATCATACAC CTAAAACAGT GGAAGAAGTT GTAGTTGAGC GAAATGAGAA ACAAGCACCC
    AATCTTCCAG AACCAAAGCC TGTGTACGCA CAGGTAGGAC AGCCAGACGT GGATTTACCT
    GTCAGTCCAT CTGATGGCGT CCTGCCCAAC TCAACTCATG AAGATGGCAT TCTTCGGCCC
    AGCATGAAGT TAGTAAAATT CAGGAAAGGA GATAGTGTGG GTCTTCGACT AGCTGGTGGA
    AACGATGTTG GCATATTTGT AGCTGGTGTT CTCGAAGATA GCCCTGCTGC CAAAGAAGGC
    TTGGAGGAGG GCGACCAGAT CCTCAGGGTA AACAATGTAG ATTTCACAAA TATCATAAGA
    GAAGAAGCCG TCCTCTTCCT GCTTGACCTC CCAAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AAGATGTTTA TCGTCGCATC GTAGAATCCG ATGTGGGTGA TTCTTTCTAT
    ATCAGAACCC ATTTTGAATA TGAGAAGGAA TCTCCCTATG GACTCAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTCGTGGA CACTCTGTAC AATGGGAAGC TGGGGTCCTG GCTTGCCATT
    CGGATTGGCA AAAACCATAA AGAGGTGGAA CGTGGCATCA TCCCAAACAA AAACAGAGCT
    GAGCAGTTAG CCAGTGTGCA GTATACACTT CCCAAGACAG CAGGGGGAGA CCGCGCCGAC
    TTCTGGAGGT TCCGCGGTCT GCGCAGCTCC AAGAGAAACC TGCGCAAGAG CCGAGAAGAC
    CTCTCAGCCC AGCCAGTCCA GACCAAGTTC CCGGCGTATG AGAGGGTGGT TCTTCGAGAA
    GCTGGATTCC TGAGGCCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGGGAGAAA
    CTGGCAAGAG AGGAGCCAGA TATTTACCAG ATCGCAAAAA GTGAACCTCG GGATGCAGGA
    ACCGACCAGC GCAGCTCTGG CATTATTCGC CTCCACACAA TAAAGCAGAT AATAGATCAG
    GACAAACATG CCTTACTGGA TGTAACGCCC AATGCAGTAG ACCGTCTGAA CTATGCCCAG
    TGGTATCCAA TCGTGGTGTT CCTTAACCCG GACTCAAAGC AGGGTGTGAA AACAATGCGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGC GCCAGGAAGT TATATGAGCG CTCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGCTGGTATG GTGCACTGAA AGAAGCAATC CAACAACAAC AGAACCAGTT GGTATGGGTT
    TCTGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCCCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAG
    ACCCTAAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACCCGGTC CTCTGAGCCT
    GTCAGAGAGG ACTCCTCTGG AATGCATCCT GACCACCAGG CATATCCTCC CTACTCACCA
    CAAGCGCAGC CACAGCCAGG CCATAGAATA GACTCCCCTG GATTTAAAAC AGCCTCTCAA
    CAGAAAGCAG AAGCCTCATC TCCAGGCCCT TACCTTTCGC CTGAAACAAA CCCGGCACCA
    TCAGCCTCTG CTGTTAATCC TCACGTAGAC TTAACTAATG TCAGACTGGA GGAGCCGAGC
    CCAGCTCCCC CCTCCTCTGA CTCACCACAC GCCGACTCTT TACGAGCTCC AGGCACTGAG
    GCCGCTCACG TGCAGCTCAA AGGTCCAGAG CCGTCCCTGT CGTCGCATGT GGGCCCAGCA
    AAGGTGTACA GGAAGGACCC CTACCCTGAG GAGATGATGA GACAGAACCA CGTCTTGAAG
    CAGCCGGCTG TGGTTCACCC TGGGCAGAGG CCAGACAAGG AGCAAGGCCT GAGCTACGAG
    CCGCAGCCTC CGTACACAGA GCGACAGGCT GGCAGGGAGT TGGAGCGGCC TGCATACAGA
    TACGATGCCT CTAGCTACCC GGAGCAGTTT GCTCGGAACT ATGACCATCG TCTGCGCTAT
    GACGACCGCC TCCTCACCTA TGAGGAACAA TGGCCCTACT ACGATGACAA GCAGCCTTAC
    CAGCCCCGGC CTCTTGATGG TCAGCCTCCC GCGGAGCCAA CAGAACGAGG GTATTTCCCC
    CGTTTTGAGG AGCCAGTGCC CCTGTCCTAT GAAGGCCGAG CGCACTACGA CCCACTGCCC
    AGGACCTCCA CCTTGCGGCA CGAAGAGCCT CCCGCCCCTG CCCCTGGGTA CGACGTGCAC
    GGAAGGTACC GGCCTGACGT GCAGCCCTAC TCCACTGCTG GCCCCAAGGT GCCCCCTGAG
    CCCAAGCAGT ACTTGGACCA GTACCCTCGG AGTTACGAGC CAGCGCCAGC CCAGGGCTTC
    CCCTCCAGGG CAGGCCCAGG CCACTACGAG CCTCTTCACA GCACCACGCT CGCTCCACCA
    CTTGGCCCCT CGTCCCAGCA CAAGCCCGAA GTCCCGCCCA CAGGCACCAA ACCCCTGCCT
    CCACCCCCAG CTCCGGCCGA GGAGGAGGAA GACCCAGCCA TGAAGCCACA ATCCGTGCTC
    ACCAGAGTGA AGATGTTTGA GAATAAGAGG TCTGCTTCCA TGGAGAACAG GAAGGATGAG
    AGCCATTCCA CTAGTTTCAA GCCCCCAGAA GTCACGTCAA AACCGGCAGG TGCTCCTGTC
    CCAGGTCCCA AAGCCCCTGC TCCGAGTCAG CTCGGTGAAC ACGACAGAGC AGCACATAGG
    ATCCCTGAAC CACAGAGGCC TCACATGAAG CCACCTGAAG ACATTGTTCG GGCAAATCAC
    TATGACCCCG AGGAGGATGA GGAGTACTAC CGGAAGCAGC TCTCCTACTT CGATCGGAGA
    AGCTTTGAGG CCAAGCCCCC CACCCACATC CCCACTAGCC ACCTCTCCGA GCCCACCAGG
    CCGGCTCACC CCCAATCTCA GCCACAGTTT GCCGGTTACT CTTCCAAGGG GAAGGCTGCT
    GAGGCCGAAG CTGTGGACAG GTCATTTGGG GAGAAGCGCT ATGACCCGCT GCAGGCGACG
    CCCCCCCCTC CGCCGCTGCC TGTTCAGTAC AGCCAGGGTG CTCAACCCAG CCCCAACACG
    GCTCTTGGCC TCCACGCACA CACCAAGGGG GCGCATGGTG AAGGTAATGC CATGTCGTTG
    GATTTTCAGA ACTCGTTAGT GTCCAAACCG GACCCACCTC CATCTCAGAA TAAGCCAGCA
    GCTTACAGAC CAGCCCCTCG AGAGGATGCT GTCCAGTCTG GCTTCTACCC ACAAAAGAGT
    TTCCCAGAGA AAGCCCCAGC TAATGGGGTC GAGCAGACGC AGAAAACCGT CACGCCAGCA
    TACAATCGAT TCACACCAAA ACCATACACA AGCTCTGCAC GGCCATTTGA ACGCAAGTTT
    GAAAGCCCTA AATTCAACCA CAATCTCTTG CCGAATGAAA CCGCACACAA ACCTGACCTG
    TCCTCCAAAG CCCCCGCTTC TCCGAAAACC CTCCTGAAGT CTGCACAGCC TCCTGAGTTT
    GACAGTGGCG TGGAGACCCT CTCTGTCCAC ACTGACAACA AGCTGAAATA CCAAGTAAAC
    AGTGTTAGCA TGGGGCCGAG AGCAGTGCCC GTGAGTCCCT CAGCCGTGGA GGAAGATGAA
    GACGAAGACG GTCACACAGT GGTGGCTACT GCCCGCGGCG TGTTTAACAG CAATGGTGGG
    GTGCTGAGCT CCATTGAGAC CGGTGTCAGC ATCATCATCC CCCAGGGAGC CATTCCCGAG
    GGGGTGGAAC AGGAGATCTA CTTCAAGGTC TGCCGCGACA ACAGCATCCT GCCGCCTTTG
    GACAAAGAGA AGGGTGAGAC CCTGCTGAGC CCCCTAGTGA TGTGTGGGCC CCATGGCCTC
    AAGTTCCTGA AGCCCGTGGA GCTGCGCCTA CCACACTGTG CGTCCATGAC TCCTGACGGT
    TGGTCTTTTG CTCTAAAATC ATCCGACTCC TCGTCGGGTG ATCCTAAAAC CTGGCAAAAC
    AAGTGTCTTC CTGGAGATCC AAATTATCTT GTTGGAGCAA ACTGTGTTTC TGTCCTGATT
    GACCACTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837775.1
    CDS2027..7318
    Translation

    Target ORF information:

    RefSeq Version XM_024982007.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 1 (TJP1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982007.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCCGCCA GGGCCGCGGC CGCCAAGAAC ACAACAATGG AGGAAACAGC TATATGGGAA 
    CAACACACGG TGACACTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGAAGAGATA ATCCACATTT CCAGAGCGGG GAAACCTCCA TCGTGATTTC AGATGTGCTG
    AAGGGGGGGC CTGCGGAAGG ACAGCTGCAG GAAAATGACC GGGTGGCAAT GGTTAATGGA
    GTCTCCATGG ATAATGTTGA GCATGCGTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TAACCATTCG AAGGAAGAAG AAAGTTCAGA TCCCAGTAAA TCGTCCTGAT
    CCTGAGCCTG TGTCTGAGAA CGAAGAGGAC AGTTACGACG AGGAAGCGCA TGACCCGAGA
    AGTGGCCGTG GGGCCCTGGC AAACAGGAGG AGCGAGAAGA GCTGGGGCCG AGATAGGAGT
    GCCAGTCGGG AGAGGAGTCT GTCCCCGCGG TCTGACAGGC GGTCTGTAGC CTCCAGTCAG
    CCTGCTAGAC CCACCAAAGT CACCCTGGTG AAGTCCCGGA AGAATGAAGA ATATGGTCTT
    CGATTGGCCA GCCATATATT TGTGAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGCGA TGTTGTATTG AAGATAAATG GTACCGTGAC GGAAAACATG
    TCACTGACAG ATGCGAAGAC GCTGATCGAG AGGTCTAAGG GCAAGTTAAA GATGGTGGTT
    CAGAGAGATG AGCGGGCTAC CCTGCTGAAT GTCCCTGACC TTTCTGACAG CATCCACTCT
    GCTGACGCCT CCGAGAGGGA CGATATTTCA GAAATCCAGT CACTGGCATC AGACCATTCA
    GGTCGATCCC ACGACCGGCC TCCCCGTCAC AGCCGCTCTC GGTCTCCTGA CCAGCGGTCC
    GAGCCTTCTG ACCATTCTAG ACACTCTCCA CAGCAGCCCA GCAGTGGCAG TCTCCGGAGC
    AGAGAGGAAG AGAGAATTTC TAAACCAGGG GCTGTCTCGA CTCCGGTAAA GCATGCAGAT
    GATCATACAC CTAAAACAGT GGAAGAAGTT GTAGTTGAGC GAAATGAGAA ACAAGCACCC
    AATCTTCCAG AACCAAAGCC TGTGTACGCA CAGGTAGGAC AGCCAGACGT GGATTTACCT
    GTCAGTCCAT CTGATGGCGT CCTGCCCAAC TCAACTCATG AAGATGGCAT TCTTCGGCCC
    AGCATGAAGT TAGTAAAATT CAGGAAAGGA GATAGTGTGG GTCTTCGACT AGCTGGTGGA
    AACGATGTTG GCATATTTGT AGCTGGTGTT CTCGAAGATA GCCCTGCTGC CAAAGAAGGC
    TTGGAGGAGG GCGACCAGAT CCTCAGGGTA AACAATGTAG ATTTCACAAA TATCATAAGA
    GAAGAAGCCG TCCTCTTCCT GCTTGACCTC CCAAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AAGATGTTTA TCGTCGCATC GTAGAATCCG ATGTGGGTGA TTCTTTCTAT
    ATCAGAACCC ATTTTGAATA TGAGAAGGAA TCTCCCTATG GACTCAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTCGTGGA CACTCTGTAC AATGGGAAGC TGGGGTCCTG GCTTGCCATT
    CGGATTGGCA AAAACCATAA AGAGGTGGAA CGTGGCATCA TCCCAAACAA AAACAGAGCT
    GAGCAGTTAG CCAGTGTGCA GTATACACTT CCCAAGACAG CAGGGGGAGA CCGCGCCGAC
    TTCTGGAGGT TCCGCGGTCT GCGCAGCTCC AAGAGAAACC TGCGCAAGAG CCGAGAAGAC
    CTCTCAGCCC AGCCAGTCCA GACCAAGTTC CCGGCGTATG AGAGGGTGGT TCTTCGAGAA
    GCTGGATTCC TGAGGCCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGGGAGAAA
    CTGGCAAGAG AGGAGCCAGA TATTTACCAG ATCGCAAAAA GTGAACCTCG GGATGCAGGA
    ACCGACCAGC GCAGCTCTGG CATTATTCGC CTCCACACAA TAAAGCAGAT AATAGATCAG
    GACAAACATG CCTTACTGGA TGTAACGCCC AATGCAGTAG ACCGTCTGAA CTATGCCCAG
    TGGTATCCAA TCGTGGTGTT CCTTAACCCG GACTCAAAGC AGGGTGTGAA AACAATGCGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGC GCCAGGAAGT TATATGAGCG CTCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGCTGGTATG GTGCACTGAA AGAAGCAATC CAACAACAAC AGAACCAGTT GGTATGGGTT
    TCTGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCCCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAG
    ACCCTAAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACCCGGTC CTCTGAGCCT
    GTCAGAGAGG ACTCCTCTGG AATGCATCCT GACCACCAGG CATATCCTCC CTACTCACCA
    CAAGCGCAGC CACAGCCAGG CCATAGAATA GACTCCCCTG GATTTAAAAC AGCCTCTCAA
    CAGAAAGCAG AAGCCTCATC TCCAGGCCCT TACCTTTCGC CTGAAACAAA CCCGGCACCA
    TCAGCCTCTG CTGTTAATCC TCACGTAGAC TTAACTAATG TCAGACTGGA GGAGCCGAGC
    CCAGCTCCCC CCTCCTCTGA CTCACCACAC GCCGACTCTT TACGAGCTCC AGGCACTGAG
    GCCGCTCACG TGCAGCTCAA AGGTCCAGAG CCGTCCCTGT CGTCGCATGT GGGCCCAGCA
    AAGGTGTACA GGAAGGACCC CTACCCTGAG GAGATGATGA GACAGAACCA CGTCTTGAAG
    CAGCCGGCTG TGGTTCACCC TGGGCAGAGG CCAGACAAGG AGCAAGGCCT GAGCTACGAG
    CCGCAGCCTC CGTACACAGA GCGACAGGCT GGCAGGGAGT TGGAGCGGCC TGCATACAGA
    TACGATGCCT CTAGCTACCC GGAGCAGTTT GCTCGGAACT ATGACCATCG TCTGCGCTAT
    GACGACCGCC TCCTCACCTA TGAGGAACAA TGGCCCTACT ACGATGACAA GCAGCCTTAC
    CAGCCCCGGC CTCTTGATGG TCAGCCTCCC GCGGAGCCAA CAGAACGAGG GTATTTCCCC
    CGTTTTGAGG AGCCAGTGCC CCTGTCCTAT GAAGGCCGAG CGCACTACGA CCCACTGCCC
    AGGACCTCCA CCTTGCGGCA CGAAGAGCCT CCCGCCCCTG CCCCTGGGTA CGACGTGCAC
    GGAAGGTACC GGCCTGACGT GCAGCCCTAC TCCACTGCTG GCCCCAAGGT GCCCCCTGAG
    CCCAAGCAGT ACTTGGACCA GTACCCTCGG AGTTACGAGC CAGCGCCAGC CCAGGGCTTC
    CCCTCCAGGG CAGGCCCAGG CCACTACGAG CCTCTTCACA GCACCACGCT CGCTCCACCA
    CTTGGCCCCT CGTCCCAGCA CAAGCCCGAA GTCCCGCCCA CAGGCACCAA ACCCCTGCCT
    CCACCCCCAG CTCCGGCCGA GGAGGAGGAA GACCCAGCCA TGAAGCCACA ATCCGTGCTC
    ACCAGAGTGA AGATGTTTGA GAATAAGAGG TCTGCTTCCA TGGAGAACAG GAAGGATGAG
    AGCCATTCCA CTAGTTTCAA GCCCCCAGAA GTCACGTCAA AACCGGCAGG TGCTCCTGTC
    CCAGGTCCCA AAGCCCCTGC TCCGAGTCAG CTCGGTGAAC ACGACAGAGC AGCACATAGG
    ATCCCTGAAC CACAGAGGCC TCACATGAAG CCACCTGAAG ACATTGTTCG GGCAAATCAC
    TATGACCCCG AGGAGGATGA GGAGTACTAC CGGAAGCAGC TCTCCTACTT CGATCGGAGA
    AGCTTTGAGG CCAAGCCCCC CACCCACATC CCCACTAGCC ACCTCTCCGA GCCCACCAGG
    CCGGCTCACC CCCAATCTCA GCCACAGTTT GCCGGTTACT CTTCCAAGGG GAAGGCTGCT
    GAGGCCGAAG CTGTGGACAG GTCATTTGGG GAGAAGCGCT ATGACCCGCT GCAGGCGACG
    CCCCCCCCTC CGCCGCTGCC TGTTCAGTAC AGCCAGGGTG CTCAACCCAG CCCCAACACG
    GCTCTTGGCC TCCACGCACA CACCAAGGGG GCGCATGGTG AAGGTAATGC CATGTCGTTG
    GATTTTCAGA ACTCGTTAGT GTCCAAACCG GACCCACCTC CATCTCAGAA TAAGCCAGCA
    GCTTACAGAC CAGCCCCTCG AGAGGATGCT GTCCAGTCTG GCTTCTACCC ACAAAAGAGT
    TTCCCAGAGA AAGCCCCAGC TAATGGGGTC GAGCAGACGC AGAAAACCGT CACGCCAGCA
    TACAATCGAT TCACACCAAA ACCATACACA AGCTCTGCAC GGCCATTTGA ACGCAAGTTT
    GAAAGCCCTA AATTCAACCA CAATCTCTTG CCGAATGAAA CCGCACACAA ACCTGACCTG
    TCCTCCAAAG CCCCCGCTTC TCCGAAAACC CTCCTGAAGT CTGCACAGCC TCCTGAGTTT
    GACAGTGGCG TGGAGACCCT CTCTGTCCAC ACTGACAACA AGCTGAAATA CCAAGTAAAC
    AGTGTTAGCA TGGGGCCGAG AGCAGTGCCC GTGAGTCCCT CAGCCGTGGA GGAAGATGAA
    GACGAAGACG GTCACACAGT GGTGGCTACT GCCCGCGGCG TGTTTAACAG CAATGGTGGG
    GTGCTGAGCT CCATTGAGAC CGGTGTCAGC ATCATCATCC CCCAGGGAGC CATTCCCGAG
    GGGGTGGAAC AGGAGATCTA CTTCAAGGTC TGCCGCGACA ACAGCATCCT GCCGCCTTTG
    GACAAAGAGA AGGGTGAGAC CCTGCTGAGC CCCCTAGTGA TGTGTGGGCC CCATGGCCTC
    AAGTTCCTGA AGCCCGTGGA GCTGCGCCTA CCACACTGTG CGTCCATGAC TCCTGACGGT
    TGGTCTTTTG CTCTAAAATC ATCCGACTCC TCGTCGGGTG ATCCTAAAAC CTGGCAAAAC
    AAGTGTCTTC CTGGAGATCC AAATTATCTT GTTGGAGCAA ACTGTGTTTC TGTCCTGATT
    GACCACTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228437
    Clone ID Related Accession (Same CDS sequence) XM_024982008.1
    Accession Version XM_024982008.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5289bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGACGTCGC CACTGGACAA GGAGAACACA ACAATGGAGG AAACAGCTAT ATGGGAACAA 
    CACACGGTGA CACTTCACAG GGCTCCTGGA TTTGGATTTG GAATTGCAAT ATCTGGTGGA
    AGAGATAATC CACATTTCCA GAGCGGGGAA ACCTCCATCG TGATTTCAGA TGTGCTGAAG
    GGGGGGCCTG CGGAAGGACA GCTGCAGGAA AATGACCGGG TGGCAATGGT TAATGGAGTC
    TCCATGGATA ATGTTGAGCA TGCGTTTGCT GTTCAGCAAC TAAGGAAAAG TGGGAAAAAT
    GCAAAAATAA CCATTCGAAG GAAGAAGAAA GTTCAGATCC CAGTAAATCG TCCTGATCCT
    GAGCCTGTGT CTGAGAACGA AGAGGACAGT TACGACGAGG AAGCGCATGA CCCGAGAAGT
    GGCCGTGGGG CCCTGGCAAA CAGGAGGAGC GAGAAGAGCT GGGGCCGAGA TAGGAGTGCC
    AGTCGGGAGA GGAGTCTGTC CCCGCGGTCT GACAGGCGGT CTGTAGCCTC CAGTCAGCCT
    GCTAGACCCA CCAAAGTCAC CCTGGTGAAG TCCCGGAAGA ATGAAGAATA TGGTCTTCGA
    TTGGCCAGCC ATATATTTGT GAAGGAAATT TCACAAGATA GTTTGGCAGC AAGAGATGGC
    AATATTCAAG AAGGCGATGT TGTATTGAAG ATAAATGGTA CCGTGACGGA AAACATGTCA
    CTGACAGATG CGAAGACGCT GATCGAGAGG TCTAAGGGCA AGTTAAAGAT GGTGGTTCAG
    AGAGATGAGC GGGCTACCCT GCTGAATGTC CCTGACCTTT CTGACAGCAT CCACTCTGCT
    GACGCCTCCG AGAGGGACGA TATTTCAGAA ATCCAGTCAC TGGCATCAGA CCATTCAGGT
    CGATCCCACG ACCGGCCTCC CCGTCACAGC CGCTCTCGGT CTCCTGACCA GCGGTCCGAG
    CCTTCTGACC ATTCTAGACA CTCTCCACAG CAGCCCAGCA GTGGCAGTCT CCGGAGCAGA
    GAGGAAGAGA GAATTTCTAA ACCAGGGGCT GTCTCGACTC CGGTAAAGCA TGCAGATGAT
    CATACACCTA AAACAGTGGA AGAAGTTGTA GTTGAGCGAA ATGAGAAACA AGCACCCAAT
    CTTCCAGAAC CAAAGCCTGT GTACGCACAG GTAGGACAGC CAGACGTGGA TTTACCTGTC
    AGTCCATCTG ATGGCGTCCT GCCCAACTCA ACTCATGAAG ATGGCATTCT TCGGCCCAGC
    ATGAAGTTAG TAAAATTCAG GAAAGGAGAT AGTGTGGGTC TTCGACTAGC TGGTGGAAAC
    GATGTTGGCA TATTTGTAGC TGGTGTTCTC GAAGATAGCC CTGCTGCCAA AGAAGGCTTG
    GAGGAGGGCG ACCAGATCCT CAGGGTAAAC AATGTAGATT TCACAAATAT CATAAGAGAA
    GAAGCCGTCC TCTTCCTGCT TGACCTCCCA AAAGGAGAAG AAGTGACCAT ATTGGCTCAG
    AAGAAGAAAG ATGTTTATCG TCGCATCGTA GAATCCGATG TGGGTGATTC TTTCTATATC
    AGAACCCATT TTGAATATGA GAAGGAATCT CCCTATGGAC TCAGTTTTAA CAAAGGAGAG
    GTGTTCCGTG TCGTGGACAC TCTGTACAAT GGGAAGCTGG GGTCCTGGCT TGCCATTCGG
    ATTGGCAAAA ACCATAAAGA GGTGGAACGT GGCATCATCC CAAACAAAAA CAGAGCTGAG
    CAGTTAGCCA GTGTGCAGTA TACACTTCCC AAGACAGCAG GGGGAGACCG CGCCGACTTC
    TGGAGGTTCC GCGGTCTGCG CAGCTCCAAG AGAAACCTGC GCAAGAGCCG AGAAGACCTC
    TCAGCCCAGC CAGTCCAGAC CAAGTTCCCG GCGTATGAGA GGGTGGTTCT TCGAGAAGCT
    GGATTCCTGA GGCCTGTAAC CATCTTTGGA CCAATAGCTG ATGTTGCCAG GGAGAAACTG
    GCAAGAGAGG AGCCAGATAT TTACCAGATC GCAAAAAGTG AACCTCGGGA TGCAGGAACC
    GACCAGCGCA GCTCTGGCAT TATTCGCCTC CACACAATAA AGCAGATAAT AGATCAGGAC
    AAACATGCCT TACTGGATGT AACGCCCAAT GCAGTAGACC GTCTGAACTA TGCCCAGTGG
    TATCCAATCG TGGTGTTCCT TAACCCGGAC TCAAAGCAGG GTGTGAAAAC AATGCGAATG
    AGGTTATGTC CAGAATCTCG GAAAAGCGCC AGGAAGTTAT ATGAGCGCTC TCATAAACTT
    CGTAAAAATA ATCACCATCT TTTTACAACT ACAATTAACT TAAATTCAAT GAATGATGGC
    TGGTATGGTG CACTGAAAGA AGCAATCCAA CAACAACAGA ACCAGTTGGT ATGGGTTTCT
    GAGGGAAAGG CGGATGGTGC TACAAGTGAT GACCTTGATT TGCATGATGA TCGTCTGTCC
    TACCTGTCAG CCCCAGGTAG TGAATACTCA ATGTATAGCA CGGACAGTAG ACACACTTCT
    GACTATGAAG ACACAGACAC AGAAGGCGGG GCCTACACTG ATCAAGAACT AGATGAGACC
    CTAAATGATG AGGTGGGGAC TCCCCCGGAG TCTGCCATTA CCCGGTCCTC TGAGCCTGTC
    AGAGAGGACT CCTCTGGAAT GCATCCTGAC CACCAGGCAT ATCCTCCCTA CTCACCACAA
    GCGCAGCCAC AGCCAGGCCA TAGAATAGAC TCCCCTGGAT TTAAAACAGC CTCTCAACAG
    AAAGCAGAAG CCTCATCTCC AGGCCCTTAC CTTTCGCCTG AAACAAACCC GGCACCATCA
    GCCTCTGCTG TTAATCCTCA CGTAGACTTA ACTAATGTCA GACTGGAGGA GCCGAGCCCA
    GCTCCCCCCT CCTCTGACTC ACCACACGCC GACTCTTTAC GAGCTCCAGG CACTGAGGCC
    GCTCACGTGC AGCTCAAAGG TCCAGAGCCG TCCCTGTCGT CGCATGTGGG CCCAGCAAAG
    GTGTACAGGA AGGACCCCTA CCCTGAGGAG ATGATGAGAC AGAACCACGT CTTGAAGCAG
    CCGGCTGTGG TTCACCCTGG GCAGAGGCCA GACAAGGAGC AAGGCCTGAG CTACGAGCCG
    CAGCCTCCGT ACACAGAGCG ACAGGCTGGC AGGGAGTTGG AGCGGCCTGC ATACAGATAC
    GATGCCTCTA GCTACCCGGA GCAGTTTGCT CGGAACTATG ACCATCGTCT GCGCTATGAC
    GACCGCCTCC TCACCTATGA GGAACAATGG CCCTACTACG ATGACAAGCA GCCTTACCAG
    CCCCGGCCTC TTGATGGTCA GCCTCCCGCG GAGCCAACAG AACGAGGGTA TTTCCCCCGT
    TTTGAGGAGC CAGTGCCCCT GTCCTATGAA GGCCGAGCGC ACTACGACCC ACTGCCCAGG
    ACCTCCACCT TGCGGCACGA AGAGCCTCCC GCCCCTGCCC CTGGGTACGA CGTGCACGGA
    AGGTACCGGC CTGACGTGCA GCCCTACTCC ACTGCTGGCC CCAAGGTGCC CCCTGAGCCC
    AAGCAGTACT TGGACCAGTA CCCTCGGAGT TACGAGCCAG CGCCAGCCCA GGGCTTCCCC
    TCCAGGGCAG GCCCAGGCCA CTACGAGCCT CTTCACAGCA CCACGCTCGC TCCACCACTT
    GGCCCCTCGT CCCAGCACAA GCCCGAAGTC CCGCCCACAG GCACCAAACC CCTGCCTCCA
    CCCCCAGCTC CGGCCGAGGA GGAGGAAGAC CCAGCCATGA AGCCACAATC CGTGCTCACC
    AGAGTGAAGA TGTTTGAGAA TAAGAGGTCT GCTTCCATGG AGAACAGGAA GGATGAGAGC
    CATTCCACTA GTTTCAAGCC CCCAGAAGTC ACGTCAAAAC CGGCAGGTGC TCCTGTCCCA
    GGTCCCAAAG CCCCTGCTCC GAGTCAGCTC GGTGAACACG ACAGAGCAGC ACATAGGATC
    CCTGAACCAC AGAGGCCTCA CATGAAGCCA CCTGAAGACA TTGTTCGGGC AAATCACTAT
    GACCCCGAGG AGGATGAGGA GTACTACCGG AAGCAGCTCT CCTACTTCGA TCGGAGAAGC
    TTTGAGGCCA AGCCCCCCAC CCACATCCCC ACTAGCCACC TCTCCGAGCC CACCAGGCCG
    GCTCACCCCC AATCTCAGCC ACAGTTTGCC GGTTACTCTT CCAAGGGGAA GGCTGCTGAG
    GCCGAAGCTG TGGACAGGTC ATTTGGGGAG AAGCGCTATG ACCCGCTGCA GGCGACGCCC
    CCCCCTCCGC CGCTGCCTGT TCAGTACAGC CAGGGTGCTC AACCCAGCCC CAACACGGCT
    CTTGGCCTCC ACGCACACAC CAAGGGGGCG CATGGTGAAG GTAATGCCAT GTCGTTGGAT
    TTTCAGAACT CGTTAGTGTC CAAACCGGAC CCACCTCCAT CTCAGAATAA GCCAGCAGCT
    TACAGACCAG CCCCTCGAGA GGATGCTGTC CAGTCTGGCT TCTACCCACA AAAGAGTTTC
    CCAGAGAAAG CCCCAGCTAA TGGGGTCGAG CAGACGCAGA AAACCGTCAC GCCAGCATAC
    AATCGATTCA CACCAAAACC ATACACAAGC TCTGCACGGC CATTTGAACG CAAGTTTGAA
    AGCCCTAAAT TCAACCACAA TCTCTTGCCG AATGAAACCG CACACAAACC TGACCTGTCC
    TCCAAAGCCC CCGCTTCTCC GAAAACCCTC CTGAAGTCTG CACAGCCTCC TGAGTTTGAC
    AGTGGCGTGG AGACCCTCTC TGTCCACACT GACAACAAGC TGAAATACCA AGTAAACAGT
    GTTAGCATGG GGCCGAGAGC AGTGCCCGTG AGTCCCTCAG CCGTGGAGGA AGATGAAGAC
    GAAGACGGTC ACACAGTGGT GGCTACTGCC CGCGGCGTGT TTAACAGCAA TGGTGGGGTG
    CTGAGCTCCA TTGAGACCGG TGTCAGCATC ATCATCCCCC AGGGAGCCAT TCCCGAGGGG
    GTGGAACAGG AGATCTACTT CAAGGTCTGC CGCGACAACA GCATCCTGCC GCCTTTGGAC
    AAAGAGAAGG GTGAGACCCT GCTGAGCCCC CTAGTGATGT GTGGGCCCCA TGGCCTCAAG
    TTCCTGAAGC CCGTGGAGCT GCGCCTACCA CACTGTGCGT CCATGACTCC TGACGGTTGG
    TCTTTTGCTC TAAAATCATC CGACTCCTCG TCGGGTGATC CTAAAACCTG GCAAAACAAG
    TGTCTTCCTG GAGATCCAAA TTATCTTGTT GGAGCAAACT GTGTTTCTGT CCTGATTGAC
    CACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837776.1
    CDS16..5304
    Translation

    Target ORF information:

    RefSeq Version XM_024982008.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 1 (TJP1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982008.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGACGTCGC CACTGGACAA GGAGAACACA ACAATGGAGG AAACAGCTAT ATGGGAACAA 
    CACACGGTGA CACTTCACAG GGCTCCTGGA TTTGGATTTG GAATTGCAAT ATCTGGTGGA
    AGAGATAATC CACATTTCCA GAGCGGGGAA ACCTCCATCG TGATTTCAGA TGTGCTGAAG
    GGGGGGCCTG CGGAAGGACA GCTGCAGGAA AATGACCGGG TGGCAATGGT TAATGGAGTC
    TCCATGGATA ATGTTGAGCA TGCGTTTGCT GTTCAGCAAC TAAGGAAAAG TGGGAAAAAT
    GCAAAAATAA CCATTCGAAG GAAGAAGAAA GTTCAGATCC CAGTAAATCG TCCTGATCCT
    GAGCCTGTGT CTGAGAACGA AGAGGACAGT TACGACGAGG AAGCGCATGA CCCGAGAAGT
    GGCCGTGGGG CCCTGGCAAA CAGGAGGAGC GAGAAGAGCT GGGGCCGAGA TAGGAGTGCC
    AGTCGGGAGA GGAGTCTGTC CCCGCGGTCT GACAGGCGGT CTGTAGCCTC CAGTCAGCCT
    GCTAGACCCA CCAAAGTCAC CCTGGTGAAG TCCCGGAAGA ATGAAGAATA TGGTCTTCGA
    TTGGCCAGCC ATATATTTGT GAAGGAAATT TCACAAGATA GTTTGGCAGC AAGAGATGGC
    AATATTCAAG AAGGCGATGT TGTATTGAAG ATAAATGGTA CCGTGACGGA AAACATGTCA
    CTGACAGATG CGAAGACGCT GATCGAGAGG TCTAAGGGCA AGTTAAAGAT GGTGGTTCAG
    AGAGATGAGC GGGCTACCCT GCTGAATGTC CCTGACCTTT CTGACAGCAT CCACTCTGCT
    GACGCCTCCG AGAGGGACGA TATTTCAGAA ATCCAGTCAC TGGCATCAGA CCATTCAGGT
    CGATCCCACG ACCGGCCTCC CCGTCACAGC CGCTCTCGGT CTCCTGACCA GCGGTCCGAG
    CCTTCTGACC ATTCTAGACA CTCTCCACAG CAGCCCAGCA GTGGCAGTCT CCGGAGCAGA
    GAGGAAGAGA GAATTTCTAA ACCAGGGGCT GTCTCGACTC CGGTAAAGCA TGCAGATGAT
    CATACACCTA AAACAGTGGA AGAAGTTGTA GTTGAGCGAA ATGAGAAACA AGCACCCAAT
    CTTCCAGAAC CAAAGCCTGT GTACGCACAG GTAGGACAGC CAGACGTGGA TTTACCTGTC
    AGTCCATCTG ATGGCGTCCT GCCCAACTCA ACTCATGAAG ATGGCATTCT TCGGCCCAGC
    ATGAAGTTAG TAAAATTCAG GAAAGGAGAT AGTGTGGGTC TTCGACTAGC TGGTGGAAAC
    GATGTTGGCA TATTTGTAGC TGGTGTTCTC GAAGATAGCC CTGCTGCCAA AGAAGGCTTG
    GAGGAGGGCG ACCAGATCCT CAGGGTAAAC AATGTAGATT TCACAAATAT CATAAGAGAA
    GAAGCCGTCC TCTTCCTGCT TGACCTCCCA AAAGGAGAAG AAGTGACCAT ATTGGCTCAG
    AAGAAGAAAG ATGTTTATCG TCGCATCGTA GAATCCGATG TGGGTGATTC TTTCTATATC
    AGAACCCATT TTGAATATGA GAAGGAATCT CCCTATGGAC TCAGTTTTAA CAAAGGAGAG
    GTGTTCCGTG TCGTGGACAC TCTGTACAAT GGGAAGCTGG GGTCCTGGCT TGCCATTCGG
    ATTGGCAAAA ACCATAAAGA GGTGGAACGT GGCATCATCC CAAACAAAAA CAGAGCTGAG
    CAGTTAGCCA GTGTGCAGTA TACACTTCCC AAGACAGCAG GGGGAGACCG CGCCGACTTC
    TGGAGGTTCC GCGGTCTGCG CAGCTCCAAG AGAAACCTGC GCAAGAGCCG AGAAGACCTC
    TCAGCCCAGC CAGTCCAGAC CAAGTTCCCG GCGTATGAGA GGGTGGTTCT TCGAGAAGCT
    GGATTCCTGA GGCCTGTAAC CATCTTTGGA CCAATAGCTG ATGTTGCCAG GGAGAAACTG
    GCAAGAGAGG AGCCAGATAT TTACCAGATC GCAAAAAGTG AACCTCGGGA TGCAGGAACC
    GACCAGCGCA GCTCTGGCAT TATTCGCCTC CACACAATAA AGCAGATAAT AGATCAGGAC
    AAACATGCCT TACTGGATGT AACGCCCAAT GCAGTAGACC GTCTGAACTA TGCCCAGTGG
    TATCCAATCG TGGTGTTCCT TAACCCGGAC TCAAAGCAGG GTGTGAAAAC AATGCGAATG
    AGGTTATGTC CAGAATCTCG GAAAAGCGCC AGGAAGTTAT ATGAGCGCTC TCATAAACTT
    CGTAAAAATA ATCACCATCT TTTTACAACT ACAATTAACT TAAATTCAAT GAATGATGGC
    TGGTATGGTG CACTGAAAGA AGCAATCCAA CAACAACAGA ACCAGTTGGT ATGGGTTTCT
    GAGGGAAAGG CGGATGGTGC TACAAGTGAT GACCTTGATT TGCATGATGA TCGTCTGTCC
    TACCTGTCAG CCCCAGGTAG TGAATACTCA ATGTATAGCA CGGACAGTAG ACACACTTCT
    GACTATGAAG ACACAGACAC AGAAGGCGGG GCCTACACTG ATCAAGAACT AGATGAGACC
    CTAAATGATG AGGTGGGGAC TCCCCCGGAG TCTGCCATTA CCCGGTCCTC TGAGCCTGTC
    AGAGAGGACT CCTCTGGAAT GCATCCTGAC CACCAGGCAT ATCCTCCCTA CTCACCACAA
    GCGCAGCCAC AGCCAGGCCA TAGAATAGAC TCCCCTGGAT TTAAAACAGC CTCTCAACAG
    AAAGCAGAAG CCTCATCTCC AGGCCCTTAC CTTTCGCCTG AAACAAACCC GGCACCATCA
    GCCTCTGCTG TTAATCCTCA CGTAGACTTA ACTAATGTCA GACTGGAGGA GCCGAGCCCA
    GCTCCCCCCT CCTCTGACTC ACCACACGCC GACTCTTTAC GAGCTCCAGG CACTGAGGCC
    GCTCACGTGC AGCTCAAAGG TCCAGAGCCG TCCCTGTCGT CGCATGTGGG CCCAGCAAAG
    GTGTACAGGA AGGACCCCTA CCCTGAGGAG ATGATGAGAC AGAACCACGT CTTGAAGCAG
    CCGGCTGTGG TTCACCCTGG GCAGAGGCCA GACAAGGAGC AAGGCCTGAG CTACGAGCCG
    CAGCCTCCGT ACACAGAGCG ACAGGCTGGC AGGGAGTTGG AGCGGCCTGC ATACAGATAC
    GATGCCTCTA GCTACCCGGA GCAGTTTGCT CGGAACTATG ACCATCGTCT GCGCTATGAC
    GACCGCCTCC TCACCTATGA GGAACAATGG CCCTACTACG ATGACAAGCA GCCTTACCAG
    CCCCGGCCTC TTGATGGTCA GCCTCCCGCG GAGCCAACAG AACGAGGGTA TTTCCCCCGT
    TTTGAGGAGC CAGTGCCCCT GTCCTATGAA GGCCGAGCGC ACTACGACCC ACTGCCCAGG
    ACCTCCACCT TGCGGCACGA AGAGCCTCCC GCCCCTGCCC CTGGGTACGA CGTGCACGGA
    AGGTACCGGC CTGACGTGCA GCCCTACTCC ACTGCTGGCC CCAAGGTGCC CCCTGAGCCC
    AAGCAGTACT TGGACCAGTA CCCTCGGAGT TACGAGCCAG CGCCAGCCCA GGGCTTCCCC
    TCCAGGGCAG GCCCAGGCCA CTACGAGCCT CTTCACAGCA CCACGCTCGC TCCACCACTT
    GGCCCCTCGT CCCAGCACAA GCCCGAAGTC CCGCCCACAG GCACCAAACC CCTGCCTCCA
    CCCCCAGCTC CGGCCGAGGA GGAGGAAGAC CCAGCCATGA AGCCACAATC CGTGCTCACC
    AGAGTGAAGA TGTTTGAGAA TAAGAGGTCT GCTTCCATGG AGAACAGGAA GGATGAGAGC
    CATTCCACTA GTTTCAAGCC CCCAGAAGTC ACGTCAAAAC CGGCAGGTGC TCCTGTCCCA
    GGTCCCAAAG CCCCTGCTCC GAGTCAGCTC GGTGAACACG ACAGAGCAGC ACATAGGATC
    CCTGAACCAC AGAGGCCTCA CATGAAGCCA CCTGAAGACA TTGTTCGGGC AAATCACTAT
    GACCCCGAGG AGGATGAGGA GTACTACCGG AAGCAGCTCT CCTACTTCGA TCGGAGAAGC
    TTTGAGGCCA AGCCCCCCAC CCACATCCCC ACTAGCCACC TCTCCGAGCC CACCAGGCCG
    GCTCACCCCC AATCTCAGCC ACAGTTTGCC GGTTACTCTT CCAAGGGGAA GGCTGCTGAG
    GCCGAAGCTG TGGACAGGTC ATTTGGGGAG AAGCGCTATG ACCCGCTGCA GGCGACGCCC
    CCCCCTCCGC CGCTGCCTGT TCAGTACAGC CAGGGTGCTC AACCCAGCCC CAACACGGCT
    CTTGGCCTCC ACGCACACAC CAAGGGGGCG CATGGTGAAG GTAATGCCAT GTCGTTGGAT
    TTTCAGAACT CGTTAGTGTC CAAACCGGAC CCACCTCCAT CTCAGAATAA GCCAGCAGCT
    TACAGACCAG CCCCTCGAGA GGATGCTGTC CAGTCTGGCT TCTACCCACA AAAGAGTTTC
    CCAGAGAAAG CCCCAGCTAA TGGGGTCGAG CAGACGCAGA AAACCGTCAC GCCAGCATAC
    AATCGATTCA CACCAAAACC ATACACAAGC TCTGCACGGC CATTTGAACG CAAGTTTGAA
    AGCCCTAAAT TCAACCACAA TCTCTTGCCG AATGAAACCG CACACAAACC TGACCTGTCC
    TCCAAAGCCC CCGCTTCTCC GAAAACCCTC CTGAAGTCTG CACAGCCTCC TGAGTTTGAC
    AGTGGCGTGG AGACCCTCTC TGTCCACACT GACAACAAGC TGAAATACCA AGTAAACAGT
    GTTAGCATGG GGCCGAGAGC AGTGCCCGTG AGTCCCTCAG CCGTGGAGGA AGATGAAGAC
    GAAGACGGTC ACACAGTGGT GGCTACTGCC CGCGGCGTGT TTAACAGCAA TGGTGGGGTG
    CTGAGCTCCA TTGAGACCGG TGTCAGCATC ATCATCCCCC AGGGAGCCAT TCCCGAGGGG
    GTGGAACAGG AGATCTACTT CAAGGTCTGC CGCGACAACA GCATCCTGCC GCCTTTGGAC
    AAAGAGAAGG GTGAGACCCT GCTGAGCCCC CTAGTGATGT GTGGGCCCCA TGGCCTCAAG
    TTCCTGAAGC CCGTGGAGCT GCGCCTACCA CACTGTGCGT CCATGACTCC TGACGGTTGG
    TCTTTTGCTC TAAAATCATC CGACTCCTCG TCGGGTGATC CTAAAACCTG GCAAAACAAG
    TGTCTTCCTG GAGATCCAAA TTATCTTGTT GGAGCAAACT GTGTTTCTGT CCTGATTGAC
    CACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228438
    Clone ID Related Accession (Same CDS sequence) XM_024982009.1
    Accession Version XM_024982009.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5268bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGGGCCC TGGCCGCGAG 
    CGGAGCGGGG ACAAGATGAA GTACCAGAAG TACCTGACGG TGCTGCAGAT GGCCATCGGC
    GTCACGCCGT CCAACCGCGG CAGCCTGCTG CCGCTCAAGA GGAGGCTGTG GGTAACGCCG
    TCCTCTGAAA ACCCCAGTGG TGCTACTTCT AGTGTCAGCC AAGGAAAGCC TTCTTTAAGA
    CGAATTAAAG GGAGACTGCA CAGGAGCAAG AGCCTCGATA GCATCGATTT CTGTGAACTC
    ACTAACACAA CAATGGAGGA AACAGCTATA TGGGAACAAC ACACGGTGAC ACTTCACAGG
    GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC ACATTTCCAG
    AGCGGGGAAA CCTCCATCGT GATTTCAGAT GTGCTGAAGG GGGGGCCTGC GGAAGGACAG
    CTGCAGGAAA ATGACCGGGT GGCAATGGTT AATGGAGTCT CCATGGATAA TGTTGAGCAT
    GCGTTTGCTG TTCAGCAACT AAGGAAAAGT GGGAAAAATG CAAAAATAAC CATTCGAAGG
    AAGAAGAAAG TTCAGATCCC AGTAAATCGT CCTGATCCTG AGCCTGTGTC TGAGAACGAA
    GAGGACAGTT ACGACGAGGA AGCGCATGAC CCGAGAAGTG GCCGTGGGGC CCTGGCAAAC
    AGGAGGAGCG AGAAGAGCTG GGGCCGAGAT AGGAGTGCCA GTCGGGAGAG GAGTCTGTCC
    CCGCGGTCTG ACAGGCGGTC TGTAGCCTCC AGTCAGCCTG CTAGACCCAC CAAAGTCACC
    CTGGTGAAGT CCCGGAAGAA TGAAGAATAT GGTCTTCGAT TGGCCAGCCA TATATTTGTG
    AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT
    GTATTGAAGA TAAATGGTAC CGTGACGGAA AACATGTCAC TGACAGATGC GAAGACGCTG
    ATCGAGAGGT CTAAGGGCAA GTTAAAGATG GTGGTTCAGA GAGATGAGCG GGCTACCCTG
    CTGAATGTCC CTGACCTTTC TGACAGCATC CACTCTGCTG ACGCCTCCGA GAGGGACGAT
    ATTTCAGAAA TCCAGTCACT GGCATCAGAC CATTCAGGTC GATCCCACGA CCGGCCTCCC
    CGTCACAGCC GCTCTCGGTC TCCTGACCAG CGGTCCGAGC CTTCTGACCA TTCTAGACAC
    TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA
    CCAGGGGCTG TCTCGACTCC GGTAAAGCAT GCAGATGATC ATACACCTAA AACAGTGGAA
    GAAGTTGTAG TTGAGCGAAA TGAGAAACAA GCACCCAATC TTCCAGAACC AAAGCCTGTG
    TACGCACAGG TAGGACAGCC AGACGTGGAT TTACCTGTCA GTCCATCTGA TGGCGTCCTG
    CCCAACTCAA CTCATGAAGA TGGCATTCTT CGGCCCAGCA TGAAGTTAGT AAAATTCAGG
    AAAGGAGATA GTGTGGGTCT TCGACTAGCT GGTGGAAACG ATGTTGGCAT ATTTGTAGCT
    GGTGTTCTCG AAGATAGCCC TGCTGCCAAA GAAGGCTTGG AGGAGGGCGA CCAGATCCTC
    AGGGTAAACA ATGTAGATTT CACAAATATC ATAAGAGAAG AAGCCGTCCT CTTCCTGCTT
    GACCTCCCAA AAGGAGAAGA AGTGACCATA TTGGCTCAGA AGAAGAAAGA TGTTTATCGT
    CGCATCGTAG AATCCGATGT GGGTGATTCT TTCTATATCA GAACCCATTT TGAATATGAG
    AAGGAATCTC CCTATGGACT CAGTTTTAAC AAAGGAGAGG TGTTCCGTGT CGTGGACACT
    CTGTACAATG GGAAGCTGGG GTCCTGGCTT GCCATTCGGA TTGGCAAAAA CCATAAAGAG
    GTGGAACGTG GCATCATCCC AAACAAAAAC AGAGCTGAGC AGTTAGCCAG TGTGCAGTAT
    ACACTTCCCA AGACAGCAGG GGGAGACCGC GCCGACTTCT GGAGGTTCCG CGGTCTGCGC
    AGCTCCAAGA GAAACCTGCG CAAGAGCCGA GAAGACCTCT CAGCCCAGCC AGTCCAGACC
    AAGTTCCCGG CGTATGAGAG GGTGGTTCTT CGAGAAGCTG GATTCCTGAG GCCTGTAACC
    ATCTTTGGAC CAATAGCTGA TGTTGCCAGG GAGAAACTGG CAAGAGAGGA GCCAGATATT
    TACCAGATCG CAAAAAGTGA ACCTCGGGAT GCAGGAACCG ACCAGCGCAG CTCTGGCATT
    ATTCGCCTCC ACACAATAAA GCAGATAATA GATCAGGACA AACATGCCTT ACTGGATGTA
    ACGCCCAATG CAGTAGACCG TCTGAACTAT GCCCAGTGGT ATCCAATCGT GGTGTTCCTT
    AACCCGGACT CAAAGCAGGG TGTGAAAACA ATGCGAATGA GGTTATGTCC AGAATCTCGG
    AAAAGCGCCA GGAAGTTATA TGAGCGCTCT CATAAACTTC GTAAAAATAA TCACCATCTT
    TTTACAACTA CAATTAACTT AAATTCAATG AATGATGGCT GGTATGGTGC ACTGAAAGAA
    GCAATCCAAC AACAACAGAA CCAGTTGGTA TGGGTTTCTG AGGGAAAGGC GGATGGTGCT
    ACAAGTGATG ACCTTGATTT GCATGATGAT CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT
    GAATACTCAA TGTATAGCAC GGACAGTAGA CACACTTCTG ACTATGAAGA CACAGACACA
    GAAGGCGGGG CCTACACTGA TCAAGAACTA GATGAGACCC TAAATGATGA GGTGGGGACT
    CCCCCGGAGT CTGCCATTAC CCGGTCCTCT GAGCCTGTCA GAGAGGACTC CTCTGGAATG
    CATCCTGACC ACCAGGCATA TCCTCCCTAC TCACCACAAG CGCAGCCACA GCCAGGCCAT
    AGAATAGACT CCCCTGGATT TAAAACAGCC TCTCAACAGG TGTACAGGAA GGACCCCTAC
    CCTGAGGAGA TGATGAGACA GAACCACGTC TTGAAGCAGC CGGCTGTGGT TCACCCTGGG
    CAGAGGCCAG ACAAGGAGCA AGGCCTGAGC TACGAGCCGC AGCCTCCGTA CACAGAGCGA
    CAGGCTGGCA GGGAGTTGGA GCGGCCTGCA TACAGATACG ATGCCTCTAG CTACCCGGAG
    CAGTTTGCTC GGAACTATGA CCATCGTCTG CGCTATGACG ACCGCCTCCT CACCTATGAG
    GAACAATGGC CCTACTACGA TGACAAGCAG CCTTACCAGC CCCGGCCTCT TGATGGTCAG
    CCTCCCGCGG AGCCAACAGA ACGAGGGTAT TTCCCCCGTT TTGAGGAGCC AGTGCCCCTG
    TCCTATGAAG GCCGAGCGCA CTACGACCCA CTGCCCAGGA CCTCCACCTT GCGGCACGAA
    GAGCCTCCCG CCCCTGCCCC TGGGTACGAC GTGCACGGAA GGTACCGGCC TGACGTGCAG
    CCCTACTCCA CTGCTGGCCC CAAGGTGCCC CCTGAGCCCA AGCAGTACTT GGACCAGTAC
    CCTCGGAGTT ACGAGCCAGC GCCAGCCCAG GGCTTCCCCT CCAGGGCAGG CCCAGGCCAC
    TACGAGCCTC TTCACAGCAC CACGCTCGCT CCACCACTTG GCCCCTCGTC CCAGCACAAG
    CCCGAAGTCC CGCCCACAGG CACCAAACCC CTGCCTCCAC CCCCAGCTCC GGCCGAGGAG
    GAGGAAGACC CAGCCATGAA GCCACAATCC GTGCTCACCA GAGTGAAGAT GTTTGAGAAT
    AAGAGGTCTG CTTCCATGGA GAACAGGAAG GATGAGAGCC ATTCCACTAG TTTCAAGCCC
    CCAGAAGTCA CGTCAAAACC GGCAGGTGCT CCTGTCCCAG GTCCCAAAGC CCCTGCTCCG
    AGTCAGCTCG GTGAACACGA CAGAGCAGCA CATAGGATCC CTGAACCACA GAGGCCTCAC
    ATGAAGCCAC CTGAAGACAT TGTTCGGGCA AATCACTATG ACCCCGAGGA GGATGAGGAG
    TACTACCGGA AGCAGCTCTC CTACTTCGAT CGGAGAAGCT TTGAGGCCAA GCCCCCCACC
    CACATCCCCA CTAGCCACCT CTCCGAGCCC ACCAGGCCGG CTCACCCCCA ATCTCAGCCA
    CAGTTTGCCG GTTACTCTTC CAAGGGGAAG GCTGCTGAGG CCGAAGCTGT GGACAGGTCA
    TTTGGGGAGA AGCGCTATGA CCCGCTGCAG GCGACGCCCC CCCCTCCGCC GCTGCCTGTT
    CAGTACAGCC AGGGTGCTCA ACCCAGCCCC AACACGGCTC TTGGCCTCCA CGCACACACC
    AAGGGGGCGC ATGGTGAAGG TAATGCCATG TCGTTGGATT TTCAGAACTC GTTAGTGTCC
    AAACCGGACC CACCTCCATC TCAGAATAAG CCAGCAGCTT ACAGACCAGC CCCTCGAGAG
    GATGCTGTCC AGTCTGGCTT CTACCCACAA AAGAGTTTCC CAGAGAAAGC CCCAGCTAAT
    GGGGTCGAGC AGACGCAGAA AACCGTCACG CCAGCATACA ATCGATTCAC ACCAAAACCA
    TACACAAGCT CTGCACGGCC ATTTGAACGC AAGTTTGAAA GCCCTAAATT CAACCACAAT
    CTCTTGCCGA ATGAAACCGC ACACAAACCT GACCTGTCCT CCAAAGCCCC CGCTTCTCCG
    AAAACCCTCC TGAAGTCTGC ACAGCCTCCT GAGTTTGACA GTGGCGTGGA GACCCTCTCT
    GTCCACACTG ACAACAAGCT GAAATACCAA GTAAACAGTG TTAGCATGGG GCCGAGAGCA
    GTGCCCGTGA GTCCCTCAGC CGTGGAGGAA GATGAAGACG AAGACGGTCA CACAGTGGTG
    GCTACTGCCC GCGGCGTGTT TAACAGCAAT GGTGGGGTGC TGAGCTCCAT TGAGACCGGT
    GTCAGCATCA TCATCCCCCA GGGAGCCATT CCCGAGGGGG TGGAACAGGA GATCTACTTC
    AAGGTCTGCC GCGACAACAG CATCCTGCCG CCTTTGGACA AAGAGAAGGG TGAGACCCTG
    CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG
    CGCCTACCAC ACTGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCAAAT
    TATCTTGTTG GAGCAAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837777.1
    CDS50..5317
    Translation

    Target ORF information:

    RefSeq Version XM_024982009.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 1 (TJP1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982009.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGGGCCC TGGCCGCGAG 
    CGGAGCGGGG ACAAGATGAA GTACCAGAAG TACCTGACGG TGCTGCAGAT GGCCATCGGC
    GTCACGCCGT CCAACCGCGG CAGCCTGCTG CCGCTCAAGA GGAGGCTGTG GGTAACGCCG
    TCCTCTGAAA ACCCCAGTGG TGCTACTTCT AGTGTCAGCC AAGGAAAGCC TTCTTTAAGA
    CGAATTAAAG GGAGACTGCA CAGGAGCAAG AGCCTCGATA GCATCGATTT CTGTGAACTC
    ACTAACACAA CAATGGAGGA AACAGCTATA TGGGAACAAC ACACGGTGAC ACTTCACAGG
    GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC ACATTTCCAG
    AGCGGGGAAA CCTCCATCGT GATTTCAGAT GTGCTGAAGG GGGGGCCTGC GGAAGGACAG
    CTGCAGGAAA ATGACCGGGT GGCAATGGTT AATGGAGTCT CCATGGATAA TGTTGAGCAT
    GCGTTTGCTG TTCAGCAACT AAGGAAAAGT GGGAAAAATG CAAAAATAAC CATTCGAAGG
    AAGAAGAAAG TTCAGATCCC AGTAAATCGT CCTGATCCTG AGCCTGTGTC TGAGAACGAA
    GAGGACAGTT ACGACGAGGA AGCGCATGAC CCGAGAAGTG GCCGTGGGGC CCTGGCAAAC
    AGGAGGAGCG AGAAGAGCTG GGGCCGAGAT AGGAGTGCCA GTCGGGAGAG GAGTCTGTCC
    CCGCGGTCTG ACAGGCGGTC TGTAGCCTCC AGTCAGCCTG CTAGACCCAC CAAAGTCACC
    CTGGTGAAGT CCCGGAAGAA TGAAGAATAT GGTCTTCGAT TGGCCAGCCA TATATTTGTG
    AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT
    GTATTGAAGA TAAATGGTAC CGTGACGGAA AACATGTCAC TGACAGATGC GAAGACGCTG
    ATCGAGAGGT CTAAGGGCAA GTTAAAGATG GTGGTTCAGA GAGATGAGCG GGCTACCCTG
    CTGAATGTCC CTGACCTTTC TGACAGCATC CACTCTGCTG ACGCCTCCGA GAGGGACGAT
    ATTTCAGAAA TCCAGTCACT GGCATCAGAC CATTCAGGTC GATCCCACGA CCGGCCTCCC
    CGTCACAGCC GCTCTCGGTC TCCTGACCAG CGGTCCGAGC CTTCTGACCA TTCTAGACAC
    TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA
    CCAGGGGCTG TCTCGACTCC GGTAAAGCAT GCAGATGATC ATACACCTAA AACAGTGGAA
    GAAGTTGTAG TTGAGCGAAA TGAGAAACAA GCACCCAATC TTCCAGAACC AAAGCCTGTG
    TACGCACAGG TAGGACAGCC AGACGTGGAT TTACCTGTCA GTCCATCTGA TGGCGTCCTG
    CCCAACTCAA CTCATGAAGA TGGCATTCTT CGGCCCAGCA TGAAGTTAGT AAAATTCAGG
    AAAGGAGATA GTGTGGGTCT TCGACTAGCT GGTGGAAACG ATGTTGGCAT ATTTGTAGCT
    GGTGTTCTCG AAGATAGCCC TGCTGCCAAA GAAGGCTTGG AGGAGGGCGA CCAGATCCTC
    AGGGTAAACA ATGTAGATTT CACAAATATC ATAAGAGAAG AAGCCGTCCT CTTCCTGCTT
    GACCTCCCAA AAGGAGAAGA AGTGACCATA TTGGCTCAGA AGAAGAAAGA TGTTTATCGT
    CGCATCGTAG AATCCGATGT GGGTGATTCT TTCTATATCA GAACCCATTT TGAATATGAG
    AAGGAATCTC CCTATGGACT CAGTTTTAAC AAAGGAGAGG TGTTCCGTGT CGTGGACACT
    CTGTACAATG GGAAGCTGGG GTCCTGGCTT GCCATTCGGA TTGGCAAAAA CCATAAAGAG
    GTGGAACGTG GCATCATCCC AAACAAAAAC AGAGCTGAGC AGTTAGCCAG TGTGCAGTAT
    ACACTTCCCA AGACAGCAGG GGGAGACCGC GCCGACTTCT GGAGGTTCCG CGGTCTGCGC
    AGCTCCAAGA GAAACCTGCG CAAGAGCCGA GAAGACCTCT CAGCCCAGCC AGTCCAGACC
    AAGTTCCCGG CGTATGAGAG GGTGGTTCTT CGAGAAGCTG GATTCCTGAG GCCTGTAACC
    ATCTTTGGAC CAATAGCTGA TGTTGCCAGG GAGAAACTGG CAAGAGAGGA GCCAGATATT
    TACCAGATCG CAAAAAGTGA ACCTCGGGAT GCAGGAACCG ACCAGCGCAG CTCTGGCATT
    ATTCGCCTCC ACACAATAAA GCAGATAATA GATCAGGACA AACATGCCTT ACTGGATGTA
    ACGCCCAATG CAGTAGACCG TCTGAACTAT GCCCAGTGGT ATCCAATCGT GGTGTTCCTT
    AACCCGGACT CAAAGCAGGG TGTGAAAACA ATGCGAATGA GGTTATGTCC AGAATCTCGG
    AAAAGCGCCA GGAAGTTATA TGAGCGCTCT CATAAACTTC GTAAAAATAA TCACCATCTT
    TTTACAACTA CAATTAACTT AAATTCAATG AATGATGGCT GGTATGGTGC ACTGAAAGAA
    GCAATCCAAC AACAACAGAA CCAGTTGGTA TGGGTTTCTG AGGGAAAGGC GGATGGTGCT
    ACAAGTGATG ACCTTGATTT GCATGATGAT CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT
    GAATACTCAA TGTATAGCAC GGACAGTAGA CACACTTCTG ACTATGAAGA CACAGACACA
    GAAGGCGGGG CCTACACTGA TCAAGAACTA GATGAGACCC TAAATGATGA GGTGGGGACT
    CCCCCGGAGT CTGCCATTAC CCGGTCCTCT GAGCCTGTCA GAGAGGACTC CTCTGGAATG
    CATCCTGACC ACCAGGCATA TCCTCCCTAC TCACCACAAG CGCAGCCACA GCCAGGCCAT
    AGAATAGACT CCCCTGGATT TAAAACAGCC TCTCAACAGG TGTACAGGAA GGACCCCTAC
    CCTGAGGAGA TGATGAGACA GAACCACGTC TTGAAGCAGC CGGCTGTGGT TCACCCTGGG
    CAGAGGCCAG ACAAGGAGCA AGGCCTGAGC TACGAGCCGC AGCCTCCGTA CACAGAGCGA
    CAGGCTGGCA GGGAGTTGGA GCGGCCTGCA TACAGATACG ATGCCTCTAG CTACCCGGAG
    CAGTTTGCTC GGAACTATGA CCATCGTCTG CGCTATGACG ACCGCCTCCT CACCTATGAG
    GAACAATGGC CCTACTACGA TGACAAGCAG CCTTACCAGC CCCGGCCTCT TGATGGTCAG
    CCTCCCGCGG AGCCAACAGA ACGAGGGTAT TTCCCCCGTT TTGAGGAGCC AGTGCCCCTG
    TCCTATGAAG GCCGAGCGCA CTACGACCCA CTGCCCAGGA CCTCCACCTT GCGGCACGAA
    GAGCCTCCCG CCCCTGCCCC TGGGTACGAC GTGCACGGAA GGTACCGGCC TGACGTGCAG
    CCCTACTCCA CTGCTGGCCC CAAGGTGCCC CCTGAGCCCA AGCAGTACTT GGACCAGTAC
    CCTCGGAGTT ACGAGCCAGC GCCAGCCCAG GGCTTCCCCT CCAGGGCAGG CCCAGGCCAC
    TACGAGCCTC TTCACAGCAC CACGCTCGCT CCACCACTTG GCCCCTCGTC CCAGCACAAG
    CCCGAAGTCC CGCCCACAGG CACCAAACCC CTGCCTCCAC CCCCAGCTCC GGCCGAGGAG
    GAGGAAGACC CAGCCATGAA GCCACAATCC GTGCTCACCA GAGTGAAGAT GTTTGAGAAT
    AAGAGGTCTG CTTCCATGGA GAACAGGAAG GATGAGAGCC ATTCCACTAG TTTCAAGCCC
    CCAGAAGTCA CGTCAAAACC GGCAGGTGCT CCTGTCCCAG GTCCCAAAGC CCCTGCTCCG
    AGTCAGCTCG GTGAACACGA CAGAGCAGCA CATAGGATCC CTGAACCACA GAGGCCTCAC
    ATGAAGCCAC CTGAAGACAT TGTTCGGGCA AATCACTATG ACCCCGAGGA GGATGAGGAG
    TACTACCGGA AGCAGCTCTC CTACTTCGAT CGGAGAAGCT TTGAGGCCAA GCCCCCCACC
    CACATCCCCA CTAGCCACCT CTCCGAGCCC ACCAGGCCGG CTCACCCCCA ATCTCAGCCA
    CAGTTTGCCG GTTACTCTTC CAAGGGGAAG GCTGCTGAGG CCGAAGCTGT GGACAGGTCA
    TTTGGGGAGA AGCGCTATGA CCCGCTGCAG GCGACGCCCC CCCCTCCGCC GCTGCCTGTT
    CAGTACAGCC AGGGTGCTCA ACCCAGCCCC AACACGGCTC TTGGCCTCCA CGCACACACC
    AAGGGGGCGC ATGGTGAAGG TAATGCCATG TCGTTGGATT TTCAGAACTC GTTAGTGTCC
    AAACCGGACC CACCTCCATC TCAGAATAAG CCAGCAGCTT ACAGACCAGC CCCTCGAGAG
    GATGCTGTCC AGTCTGGCTT CTACCCACAA AAGAGTTTCC CAGAGAAAGC CCCAGCTAAT
    GGGGTCGAGC AGACGCAGAA AACCGTCACG CCAGCATACA ATCGATTCAC ACCAAAACCA
    TACACAAGCT CTGCACGGCC ATTTGAACGC AAGTTTGAAA GCCCTAAATT CAACCACAAT
    CTCTTGCCGA ATGAAACCGC ACACAAACCT GACCTGTCCT CCAAAGCCCC CGCTTCTCCG
    AAAACCCTCC TGAAGTCTGC ACAGCCTCCT GAGTTTGACA GTGGCGTGGA GACCCTCTCT
    GTCCACACTG ACAACAAGCT GAAATACCAA GTAAACAGTG TTAGCATGGG GCCGAGAGCA
    GTGCCCGTGA GTCCCTCAGC CGTGGAGGAA GATGAAGACG AAGACGGTCA CACAGTGGTG
    GCTACTGCCC GCGGCGTGTT TAACAGCAAT GGTGGGGTGC TGAGCTCCAT TGAGACCGGT
    GTCAGCATCA TCATCCCCCA GGGAGCCATT CCCGAGGGGG TGGAACAGGA GATCTACTTC
    AAGGTCTGCC GCGACAACAG CATCCTGCCG CCTTTGGACA AAGAGAAGGG TGAGACCCTG
    CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG
    CGCCTACCAC ACTGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCAAAT
    TATCTTGTTG GAGCAAACTG TGTTTCTGTC CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228439
    Clone ID Related Accession (Same CDS sequence) XM_024982010.1
    Accession Version XM_024982010.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5253bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCCGCCA GGGCCGCGGC CGCCAAGAAC ACAACAATGG AGGAAACAGC TATATGGGAA 
    CAACACACGG TGACACTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGAAGAGATA ATCCACATTT CCAGAGCGGG GAAACCTCCA TCGTGATTTC AGATGTGCTG
    AAGGGGGGGC CTGCGGAAGG ACAGCTGCAG GAAAATGACC GGGTGGCAAT GGTTAATGGA
    GTCTCCATGG ATAATGTTGA GCATGCGTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TAACCATTCG AAGGAAGAAG AAAGTTCAGA TCCCAGTAAA TCGTCCTGAT
    CCTGAGCCTG TGTCTGAGAA CGAAGAGGAC AGTTACGACG AGGAAGCGCA TGACCCGAGA
    AGTGGCCGTG GGGCCCTGGC AAACAGGAGG AGCGAGAAGA GCTGGGGCCG AGATAGGAGT
    GCCAGTCGGG AGAGGAGTCT GTCCCCGCGG TCTGACAGGC GGTCTGTAGC CTCCAGTCAG
    CCTGCTAGAC CCACCAAAGT CACCCTGGTG AAGTCCCGGA AGAATGAAGA ATATGGTCTT
    CGATTGGCCA GCCATATATT TGTGAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGCGA TGTTGTATTG AAGATAAATG GTACCGTGAC GGAAAACATG
    TCACTGACAG ATGCGAAGAC GCTGATCGAG AGGTCTAAGG GCAAGTTAAA GATGGTGGTT
    CAGAGAGATG AGCGGGCTAC CCTGCTGAAT GTCCCTGACC TTTCTGACAG CATCCACTCT
    GCTGACGCCT CCGAGAGGGA CGATATTTCA GAAATCCAGT CACTGGCATC AGACCATTCA
    GGTCGATCCC ACGACCGGCC TCCCCGTCAC AGCCGCTCTC GGTCTCCTGA CCAGCGGTCC
    GAGCCTTCTG ACCATTCTAG ACACTCTCCA CAGCAGCCCA GCAGTGGCAG TCTCCGGAGC
    AGAGAGGAAG AGAGAATTTC TAAACCAGGG GCTGTCTCGA CTCCGGTAAA GCATGCAGAT
    GATCATACAC CTAAAACAGT GGAAGAAGTT GTAGTTGAGC GAAATGAGAA ACAAGCACCC
    AATCTTCCAG AACCAAAGCC TGTGTACGCA CAGGTAGGAC AGCCAGACGT GGATTTACCT
    GTCAGTCCAT CTGATGGCGT CCTGCCCAAC TCAACTCATG AAGATGGCAT TCTTCGGCCC
    AGCATGAAGT TAGTAAAATT CAGGAAAGGA GATAGTGTGG GTCTTCGACT AGCTGGTGGA
    AACGATGTTG GCATATTTGT AGCTGGTGTT CTCGAAGATA GCCCTGCTGC CAAAGAAGGC
    TTGGAGGAGG GCGACCAGAT CCTCAGGGTA AACAATGTAG ATTTCACAAA TATCATAAGA
    GAAGAAGCCG TCCTCTTCCT GCTTGACCTC CCAAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AAGATGTTTA TCGTCGCATC GTAGAATCCG ATGTGGGTGA TTCTTTCTAT
    ATCAGAACCC ATTTTGAATA TGAGAAGGAA TCTCCCTATG GACTCAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTCGTGGA CACTCTGTAC AATGGGAAGC TGGGGTCCTG GCTTGCCATT
    CGGATTGGCA AAAACCATAA AGAGGTGGAA CGTGGCATCA TCCCAAACAA AAACAGAGCT
    GAGCAGTTAG CCAGTGTGCA GTATACACTT CCCAAGACAG CAGGGGGAGA CCGCGCCGAC
    TTCTGGAGGT TCCGCGGTCT GCGCAGCTCC AAGAGAAACC TGCGCAAGAG CCGAGAAGAC
    CTCTCAGCCC AGCCAGTCCA GACCAAGTTC CCGGCGTATG AGAGGGTGGT TCTTCGAGAA
    GCTGGATTCC TGAGGCCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGGGAGAAA
    CTGGCAAGAG AGGAGCCAGA TATTTACCAG ATCGCAAAAA GTGAACCTCG GGATGCAGGA
    ACCGACCAGC GCAGCTCTGG CATTATTCGC CTCCACACAA TAAAGCAGAT AATAGATCAG
    GACAAACATG CCTTACTGGA TGTAACGCCC AATGCAGTAG ACCGTCTGAA CTATGCCCAG
    TGGTATCCAA TCGTGGTGTT CCTTAACCCG GACTCAAAGC AGGGTGTGAA AACAATGCGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGC GCCAGGAAGT TATATGAGCG CTCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGCTGGTATG GTGCACTGAA AGAAGCAATC CAACAACAAC AGAACCAGTT GGTATGGGTT
    TCTGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCCCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAG
    ACCCTAAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACCCGGTC CTCTGAGCCT
    GTCAGAGAGG ACTCCTCTGG AATGCATCCT GACCACCAGG CATATCCTCC CTACTCACCA
    CAAGCGCAGC CACAGCCAGG CCATAGAATA GACTCCCCTG GATTTAAAAC AGCCTCTCAA
    CAGAAAGCAG AAGCCTCATC TCCAGGCCCT TACCTTTCGC CTGAAACAAA CCCGGCACCA
    TCAGCCTCTG CTGTTAATCC TCACGTAGAC TTAACTAATG TCAGACTGGA GGAGCCGAGC
    CCAGCTCCCC CCTCCTCTGA CTCACCACAC GCCGACTCTT TACGAGCTCC AGGCACTGAG
    GCCGCTCACG TGCAGCTCAA AGGTCCAGAG CCGTCCCTGT CGTCGCATGT GGGCCCAGCA
    AAGGTGTACA GGAAGGACCC CTACCCTGAG GAGATGATGA GACAGAACCA CGTCTTGAAG
    CAGCCGGCTG TGGTTCACCC TGGGCAGAGG CCAGACAAGG AGCAAGGCCT GAGCTACGAG
    CCGCAGCCTC CGTACACAGA GCGACAGGCT GGCAGGGAGT TGGAGCGGCC TGCATACAGA
    TACGATGCCT CTAGCTACCC GGAGCAGTTT GCTCGGAACT ATGACCATCG TCTGCGCTAT
    GACGACCGCC TCCTCACCTA TGAGGAACAA TGGCCCTACT ACGATGACAA GCAGCCTTAC
    CAGCCCCGGC CTCTTGATGG TCAGCCTCCC GCGGAGCCAA CAGAACGAGG GTATTTCCCC
    CGTTTTGAGG AGCCAGTGCC CCTGTCCTAT GAAGGCCGAG CGCACTACGA CCCACTGCCC
    AGGACCTCCA CCTTGCGGCA CGAAGAGCCT CCCGCCCCTG CCCCTGGGTA CGACGTGCAC
    GGAAGGTACC GGCCTGACGT GCAGCCCTAC TCCACTGCTG GCCCCAAGGT GCCCCCTGAG
    CCCAAGCAGT ACTTGGACCA GTACCCTCGG AGTTACGAGC CAGCGCCAGC CCAGGGCTTC
    CCCTCCAGGG CAGGCCCAGG CCACTACGAG CCTCTTCACA GCACCACGCT CGCTCCACCA
    CTTGGCCCCT CGTCCCAGCA CAAGCCCGAA GTCCCGCCCA CAGGCACCAA ACCCCTGCCT
    CCACCCCCAG CTCCGGCCGA GGAGGAGGAA GACCCAGCCA TGAAGCCACA ATCCGTGCTC
    ACCAGAGTGA AGATGTTTGA GAATAAGAGG TCTGCTTCCA TGGAGAACAG GAAGGATGAG
    AGCCATTCCA CTAGTTTCAA GCCCCCAGAA GTCACGTCAA AACCGGCAGG TGCTCCTGTC
    CCAGGTCCCA AAGCCCCTGC TCCGAGTCAG CTCGGTGAAC ACGACAGAGC AGCACATAGG
    ATCCCTGAAC CACAGAGGCC TCACATGAAG CCACCTGAAG ACATTGTTCG GGCAAATCAC
    TATGACCCCG AGGAGGATGA GGAGTACTAC CGGAAGCAGC TCTCCTACTT CGATCGGAGA
    AGCTTTGAGG CCAAGCCCCC CACCCACATC CCCACTAGCC ACCTCTCCGA GCCCACCAGG
    CCGGCTCACC CCCAATCTCA GCCACAGTTT GCCGGTTACT CTTCCAAGGG GAAGGCTGCT
    GAGGCCGAAG CTGTGGACAG GTCATTTGGG GAGAAGCGCT ATGACCCGCT GCAGGCGACG
    CCCCCCCCTC CGCCGCTGCC TGTTCAGTAC AGCCAGGGTG CTCAACCCAG CCCCAACACG
    GCTCTTGGCC TCCACGCACA CACCAAGGGG GCGCATGGTG AAGGTAATGC CATGTCGTTG
    GATTTTCAGA ACTCGTTAGT GTCCAAACCG GACCCACCTC CATCTCAGAA TAAGCCAGCA
    GCTTACAGAC CAGCCCCTCG AGAGGATGCT GTCCAGTCTG GCTTCTACCC ACAAAAGAGT
    TTCCCAGAGA AAGCCCCAGC TAATGGGGTC GAGCAGACGC AGAAAACCGT CACGCCAGCA
    TACAATCGAT TCACACCAAA ACCATACACA AGCTCTGCAC GGCCATTTGA ACGCAAGTTT
    GAAAGCCCTA AATTCAACCA CAATCTCTTG CCGAATGAAA CCGCACACAA ACCTGACCTG
    TCCTCCAAAG CCCCCGCTTC TCCGAAAACC CTCCTGAAGT CTGCACAGCC TCCTGAGTTT
    GACAGTGGCG TGGAGACCCT CTCTGTCCAC ACTGACAACA AGCTGAAATA CCAAGTAAAC
    AGTGTTAGCA TGGGGCCGAG AGCAGTGCCC GTGAGTCCCT CAGCCGTGGA GGAAGATGAA
    GACGAAGACG GTCACACAGT GGTGGCTACT GCCCGCGGCG TGTTTAACAG CAATGGTGGG
    GTGCTGAGCT CCATTGAGAC CGGTGTCAGC ATCATCATCC CCCAGGGAGC CATTCCCGAG
    GGGGTGGAAC AGGAGATCTA CTTCAAGGTC TGCCGCGACA ACAGCATCCT GCCGCCTTTG
    GACAAAGAGA AGGGTGAGAC CCTGCTGAGC CCCCTAGTGA TGTGTGGGCC CCATGGCCTC
    AAGTTCCTGA AGCCCGTGGA GCTGCGCCTA CCACACTGTG CGTCCATGAC TCCTGACGGT
    GATCCTAAAA CCTGGCAAAA CAAGTGTCTT CCTGGAGATC CAAATTATCT TGTTGGAGCA
    AACTGTGTTT CTGTCCTGAT TGACCACTTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837778.1
    CDS2027..7279
    Translation

    Target ORF information:

    RefSeq Version XM_024982010.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 1 (TJP1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982010.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCCGCCA GGGCCGCGGC CGCCAAGAAC ACAACAATGG AGGAAACAGC TATATGGGAA 
    CAACACACGG TGACACTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGAAGAGATA ATCCACATTT CCAGAGCGGG GAAACCTCCA TCGTGATTTC AGATGTGCTG
    AAGGGGGGGC CTGCGGAAGG ACAGCTGCAG GAAAATGACC GGGTGGCAAT GGTTAATGGA
    GTCTCCATGG ATAATGTTGA GCATGCGTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TAACCATTCG AAGGAAGAAG AAAGTTCAGA TCCCAGTAAA TCGTCCTGAT
    CCTGAGCCTG TGTCTGAGAA CGAAGAGGAC AGTTACGACG AGGAAGCGCA TGACCCGAGA
    AGTGGCCGTG GGGCCCTGGC AAACAGGAGG AGCGAGAAGA GCTGGGGCCG AGATAGGAGT
    GCCAGTCGGG AGAGGAGTCT GTCCCCGCGG TCTGACAGGC GGTCTGTAGC CTCCAGTCAG
    CCTGCTAGAC CCACCAAAGT CACCCTGGTG AAGTCCCGGA AGAATGAAGA ATATGGTCTT
    CGATTGGCCA GCCATATATT TGTGAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGCGA TGTTGTATTG AAGATAAATG GTACCGTGAC GGAAAACATG
    TCACTGACAG ATGCGAAGAC GCTGATCGAG AGGTCTAAGG GCAAGTTAAA GATGGTGGTT
    CAGAGAGATG AGCGGGCTAC CCTGCTGAAT GTCCCTGACC TTTCTGACAG CATCCACTCT
    GCTGACGCCT CCGAGAGGGA CGATATTTCA GAAATCCAGT CACTGGCATC AGACCATTCA
    GGTCGATCCC ACGACCGGCC TCCCCGTCAC AGCCGCTCTC GGTCTCCTGA CCAGCGGTCC
    GAGCCTTCTG ACCATTCTAG ACACTCTCCA CAGCAGCCCA GCAGTGGCAG TCTCCGGAGC
    AGAGAGGAAG AGAGAATTTC TAAACCAGGG GCTGTCTCGA CTCCGGTAAA GCATGCAGAT
    GATCATACAC CTAAAACAGT GGAAGAAGTT GTAGTTGAGC GAAATGAGAA ACAAGCACCC
    AATCTTCCAG AACCAAAGCC TGTGTACGCA CAGGTAGGAC AGCCAGACGT GGATTTACCT
    GTCAGTCCAT CTGATGGCGT CCTGCCCAAC TCAACTCATG AAGATGGCAT TCTTCGGCCC
    AGCATGAAGT TAGTAAAATT CAGGAAAGGA GATAGTGTGG GTCTTCGACT AGCTGGTGGA
    AACGATGTTG GCATATTTGT AGCTGGTGTT CTCGAAGATA GCCCTGCTGC CAAAGAAGGC
    TTGGAGGAGG GCGACCAGAT CCTCAGGGTA AACAATGTAG ATTTCACAAA TATCATAAGA
    GAAGAAGCCG TCCTCTTCCT GCTTGACCTC CCAAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AAGATGTTTA TCGTCGCATC GTAGAATCCG ATGTGGGTGA TTCTTTCTAT
    ATCAGAACCC ATTTTGAATA TGAGAAGGAA TCTCCCTATG GACTCAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTCGTGGA CACTCTGTAC AATGGGAAGC TGGGGTCCTG GCTTGCCATT
    CGGATTGGCA AAAACCATAA AGAGGTGGAA CGTGGCATCA TCCCAAACAA AAACAGAGCT
    GAGCAGTTAG CCAGTGTGCA GTATACACTT CCCAAGACAG CAGGGGGAGA CCGCGCCGAC
    TTCTGGAGGT TCCGCGGTCT GCGCAGCTCC AAGAGAAACC TGCGCAAGAG CCGAGAAGAC
    CTCTCAGCCC AGCCAGTCCA GACCAAGTTC CCGGCGTATG AGAGGGTGGT TCTTCGAGAA
    GCTGGATTCC TGAGGCCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGGGAGAAA
    CTGGCAAGAG AGGAGCCAGA TATTTACCAG ATCGCAAAAA GTGAACCTCG GGATGCAGGA
    ACCGACCAGC GCAGCTCTGG CATTATTCGC CTCCACACAA TAAAGCAGAT AATAGATCAG
    GACAAACATG CCTTACTGGA TGTAACGCCC AATGCAGTAG ACCGTCTGAA CTATGCCCAG
    TGGTATCCAA TCGTGGTGTT CCTTAACCCG GACTCAAAGC AGGGTGTGAA AACAATGCGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGC GCCAGGAAGT TATATGAGCG CTCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGCTGGTATG GTGCACTGAA AGAAGCAATC CAACAACAAC AGAACCAGTT GGTATGGGTT
    TCTGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCCCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAG
    ACCCTAAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACCCGGTC CTCTGAGCCT
    GTCAGAGAGG ACTCCTCTGG AATGCATCCT GACCACCAGG CATATCCTCC CTACTCACCA
    CAAGCGCAGC CACAGCCAGG CCATAGAATA GACTCCCCTG GATTTAAAAC AGCCTCTCAA
    CAGAAAGCAG AAGCCTCATC TCCAGGCCCT TACCTTTCGC CTGAAACAAA CCCGGCACCA
    TCAGCCTCTG CTGTTAATCC TCACGTAGAC TTAACTAATG TCAGACTGGA GGAGCCGAGC
    CCAGCTCCCC CCTCCTCTGA CTCACCACAC GCCGACTCTT TACGAGCTCC AGGCACTGAG
    GCCGCTCACG TGCAGCTCAA AGGTCCAGAG CCGTCCCTGT CGTCGCATGT GGGCCCAGCA
    AAGGTGTACA GGAAGGACCC CTACCCTGAG GAGATGATGA GACAGAACCA CGTCTTGAAG
    CAGCCGGCTG TGGTTCACCC TGGGCAGAGG CCAGACAAGG AGCAAGGCCT GAGCTACGAG
    CCGCAGCCTC CGTACACAGA GCGACAGGCT GGCAGGGAGT TGGAGCGGCC TGCATACAGA
    TACGATGCCT CTAGCTACCC GGAGCAGTTT GCTCGGAACT ATGACCATCG TCTGCGCTAT
    GACGACCGCC TCCTCACCTA TGAGGAACAA TGGCCCTACT ACGATGACAA GCAGCCTTAC
    CAGCCCCGGC CTCTTGATGG TCAGCCTCCC GCGGAGCCAA CAGAACGAGG GTATTTCCCC
    CGTTTTGAGG AGCCAGTGCC CCTGTCCTAT GAAGGCCGAG CGCACTACGA CCCACTGCCC
    AGGACCTCCA CCTTGCGGCA CGAAGAGCCT CCCGCCCCTG CCCCTGGGTA CGACGTGCAC
    GGAAGGTACC GGCCTGACGT GCAGCCCTAC TCCACTGCTG GCCCCAAGGT GCCCCCTGAG
    CCCAAGCAGT ACTTGGACCA GTACCCTCGG AGTTACGAGC CAGCGCCAGC CCAGGGCTTC
    CCCTCCAGGG CAGGCCCAGG CCACTACGAG CCTCTTCACA GCACCACGCT CGCTCCACCA
    CTTGGCCCCT CGTCCCAGCA CAAGCCCGAA GTCCCGCCCA CAGGCACCAA ACCCCTGCCT
    CCACCCCCAG CTCCGGCCGA GGAGGAGGAA GACCCAGCCA TGAAGCCACA ATCCGTGCTC
    ACCAGAGTGA AGATGTTTGA GAATAAGAGG TCTGCTTCCA TGGAGAACAG GAAGGATGAG
    AGCCATTCCA CTAGTTTCAA GCCCCCAGAA GTCACGTCAA AACCGGCAGG TGCTCCTGTC
    CCAGGTCCCA AAGCCCCTGC TCCGAGTCAG CTCGGTGAAC ACGACAGAGC AGCACATAGG
    ATCCCTGAAC CACAGAGGCC TCACATGAAG CCACCTGAAG ACATTGTTCG GGCAAATCAC
    TATGACCCCG AGGAGGATGA GGAGTACTAC CGGAAGCAGC TCTCCTACTT CGATCGGAGA
    AGCTTTGAGG CCAAGCCCCC CACCCACATC CCCACTAGCC ACCTCTCCGA GCCCACCAGG
    CCGGCTCACC CCCAATCTCA GCCACAGTTT GCCGGTTACT CTTCCAAGGG GAAGGCTGCT
    GAGGCCGAAG CTGTGGACAG GTCATTTGGG GAGAAGCGCT ATGACCCGCT GCAGGCGACG
    CCCCCCCCTC CGCCGCTGCC TGTTCAGTAC AGCCAGGGTG CTCAACCCAG CCCCAACACG
    GCTCTTGGCC TCCACGCACA CACCAAGGGG GCGCATGGTG AAGGTAATGC CATGTCGTTG
    GATTTTCAGA ACTCGTTAGT GTCCAAACCG GACCCACCTC CATCTCAGAA TAAGCCAGCA
    GCTTACAGAC CAGCCCCTCG AGAGGATGCT GTCCAGTCTG GCTTCTACCC ACAAAAGAGT
    TTCCCAGAGA AAGCCCCAGC TAATGGGGTC GAGCAGACGC AGAAAACCGT CACGCCAGCA
    TACAATCGAT TCACACCAAA ACCATACACA AGCTCTGCAC GGCCATTTGA ACGCAAGTTT
    GAAAGCCCTA AATTCAACCA CAATCTCTTG CCGAATGAAA CCGCACACAA ACCTGACCTG
    TCCTCCAAAG CCCCCGCTTC TCCGAAAACC CTCCTGAAGT CTGCACAGCC TCCTGAGTTT
    GACAGTGGCG TGGAGACCCT CTCTGTCCAC ACTGACAACA AGCTGAAATA CCAAGTAAAC
    AGTGTTAGCA TGGGGCCGAG AGCAGTGCCC GTGAGTCCCT CAGCCGTGGA GGAAGATGAA
    GACGAAGACG GTCACACAGT GGTGGCTACT GCCCGCGGCG TGTTTAACAG CAATGGTGGG
    GTGCTGAGCT CCATTGAGAC CGGTGTCAGC ATCATCATCC CCCAGGGAGC CATTCCCGAG
    GGGGTGGAAC AGGAGATCTA CTTCAAGGTC TGCCGCGACA ACAGCATCCT GCCGCCTTTG
    GACAAAGAGA AGGGTGAGAC CCTGCTGAGC CCCCTAGTGA TGTGTGGGCC CCATGGCCTC
    AAGTTCCTGA AGCCCGTGGA GCTGCGCCTA CCACACTGTG CGTCCATGAC TCCTGACGGT
    GATCCTAAAA CCTGGCAAAA CAAGTGTCTT CCTGGAGATC CAAATTATCT TGTTGGAGCA
    AACTGTGTTT CTGTCCTGAT TGACCACTTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228440
    Clone ID Related Accession (Same CDS sequence) XM_024982011.1
    Accession Version XM_024982011.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCCGCCA GGGCCGCGGC CGCCAAGGCT CCTGGATTTG GATTTGGAAT TGCAATATCT 
    GGTGGAAGAG ATAATCCACA TTTCCAGAGC GGGGAAACCT CCATCGTGAT TTCAGATGTG
    CTGAAGGGGG GGCCTGCGGA AGGACAGCTG CAGGAAAATG ACCGGGTGGC AATGGTTAAT
    GGAGTCTCCA TGGATAATGT TGAGCATGCG TTTGCTGTTC AGCAACTAAG GAAAAGTGGG
    AAAAATGCAA AAATAACCAT TCGAAGGAAG AAGAAAGTTC AGATCCCAGT AAATCGTCCT
    GATCCTGAGC CTGTGTCTGA GAACGAAGAG GACAGTTACG ACGAGGAAGC GCATGACCCG
    AGAAGTGGCC GTGGGGCCCT GGCAAACAGG AGGAGCGAGA AGAGCTGGGG CCGAGATAGG
    AGTGCCAGTC GGGAGAGGAG TCTGTCCCCG CGGTCTGACA GGCGGTCTGT AGCCTCCAGT
    CAGCCTGCTA GACCCACCAA AGTCACCCTG GTGAAGTCCC GGAAGAATGA AGAATATGGT
    CTTCGATTGG CCAGCCATAT ATTTGTGAAG GAAATTTCAC AAGATAGTTT GGCAGCAAGA
    GATGGCAATA TTCAAGAAGG CGATGTTGTA TTGAAGATAA ATGGTACCGT GACGGAAAAC
    ATGTCACTGA CAGATGCGAA GACGCTGATC GAGAGGTCTA AGGGCAAGTT AAAGATGGTG
    GTTCAGAGAG ATGAGCGGGC TACCCTGCTG AATGTCCCTG ACCTTTCTGA CAGCATCCAC
    TCTGCTGACG CCTCCGAGAG GGACGATATT TCAGAAATCC AGTCACTGGC ATCAGACCAT
    TCAGGTCGAT CCCACGACCG GCCTCCCCGT CACAGCCGCT CTCGGTCTCC TGACCAGCGG
    TCCGAGCCTT CTGACCATTC TAGACACTCT CCACAGCAGC CCAGCAGTGG CAGTCTCCGG
    AGCAGAGAGG AAGAGAGAAT TTCTAAACCA GGGGCTGTCT CGACTCCGGT AAAGCATGCA
    GATGATCATA CACCTAAAAC AGTGGAAGAA GTTGTAGTTG AGCGAAATGA GAAACAAGCA
    CCCAATCTTC CAGAACCAAA GCCTGTGTAC GCACAGGTAG GACAGCCAGA CGTGGATTTA
    CCTGTCAGTC CATCTGATGG CGTCCTGCCC AACTCAACTC ATGAAGATGG CATTCTTCGG
    CCCAGCATGA AGTTAGTAAA ATTCAGGAAA GGAGATAGTG TGGGTCTTCG ACTAGCTGGT
    GGAAACGATG TTGGCATATT TGTAGCTGGT GTTCTCGAAG ATAGCCCTGC TGCCAAAGAA
    GGCTTGGAGG AGGGCGACCA GATCCTCAGG GTAAACAATG TAGATTTCAC AAATATCATA
    AGAGAAGAAG CCGTCCTCTT CCTGCTTGAC CTCCCAAAAG GAGAAGAAGT GACCATATTG
    GCTCAGAAGA AGAAAGATGT TTATCGTCGC ATCGTAGAAT CCGATGTGGG TGATTCTTTC
    TATATCAGAA CCCATTTTGA ATATGAGAAG GAATCTCCCT ATGGACTCAG TTTTAACAAA
    GGAGAGGTGT TCCGTGTCGT GGACACTCTG TACAATGGGA AGCTGGGGTC CTGGCTTGCC
    ATTCGGATTG GCAAAAACCA TAAAGAGGTG GAACGTGGCA TCATCCCAAA CAAAAACAGA
    GCTGAGCAGT TAGCCAGTGT GCAGTATACA CTTCCCAAGA CAGCAGGGGG AGACCGCGCC
    GACTTCTGGA GGTTCCGCGG TCTGCGCAGC TCCAAGAGAA ACCTGCGCAA GAGCCGAGAA
    GACCTCTCAG CCCAGCCAGT CCAGACCAAG TTCCCGGCGT ATGAGAGGGT GGTTCTTCGA
    GAAGCTGGAT TCCTGAGGCC TGTAACCATC TTTGGACCAA TAGCTGATGT TGCCAGGGAG
    AAACTGGCAA GAGAGGAGCC AGATATTTAC CAGATCGCAA AAAGTGAACC TCGGGATGCA
    GGAACCGACC AGCGCAGCTC TGGCATTATT CGCCTCCACA CAATAAAGCA GATAATAGAT
    CAGGACAAAC ATGCCTTACT GGATGTAACG CCCAATGCAG TAGACCGTCT GAACTATGCC
    CAGTGGTATC CAATCGTGGT GTTCCTTAAC CCGGACTCAA AGCAGGGTGT GAAAACAATG
    CGAATGAGGT TATGTCCAGA ATCTCGGAAA AGCGCCAGGA AGTTATATGA GCGCTCTCAT
    AAACTTCGTA AAAATAATCA CCATCTTTTT ACAACTACAA TTAACTTAAA TTCAATGAAT
    GATGGCTGGT ATGGTGCACT GAAAGAAGCA ATCCAACAAC AACAGAACCA GTTGGTATGG
    GTTTCTGAGG GAAAGGCGGA TGGTGCTACA AGTGATGACC TTGATTTGCA TGATGATCGT
    CTGTCCTACC TGTCAGCCCC AGGTAGTGAA TACTCAATGT ATAGCACGGA CAGTAGACAC
    ACTTCTGACT ATGAAGACAC AGACACAGAA GGCGGGGCCT ACACTGATCA AGAACTAGAT
    GAGACCCTAA ATGATGAGGT GGGGACTCCC CCGGAGTCTG CCATTACCCG GTCCTCTGAG
    CCTGTCAGAG AGGACTCCTC TGGAATGCAT CCTGACCACC AGGCATATCC TCCCTACTCA
    CCACAAGCGC AGCCACAGCC AGGCCATAGA ATAGACTCCC CTGGATTTAA AACAGCCTCT
    CAACAGAAAG CAGAAGCCTC ATCTCCAGGC CCTTACCTTT CGCCTGAAAC AAACCCGGCA
    CCATCAGCCT CTGCTGTTAA TCCTCACGTA GACTTAACTA ATGTCAGACT GGAGGAGCCG
    AGCCCAGCTC CCCCCTCCTC TGACTCACCA CACGCCGACT CTTTACGAGC TCCAGGCACT
    GAGGCCGCTC ACGTGCAGCT CAAAGGTCCA GAGCCGTCCC TGTCGTCGCA TGTGGGCCCA
    GCAAAGGTGT ACAGGAAGGA CCCCTACCCT GAGGAGATGA TGAGACAGAA CCACGTCTTG
    AAGCAGCCGG CTGTGGTTCA CCCTGGGCAG AGGCCAGACA AGGAGCAAGG CCTGAGCTAC
    GAGCCGCAGC CTCCGTACAC AGAGCGACAG GCTGGCAGGG AGTTGGAGCG GCCTGCATAC
    AGATACGATG CCTCTAGCTA CCCGGAGCAG TTTGCTCGGA ACTATGACCA TCGTCTGCGC
    TATGACGACC GCCTCCTCAC CTATGAGGAA CAATGGCCCT ACTACGATGA CAAGCAGCCT
    TACCAGCCCC GGCCTCTTGA TGGTCAGCCT CCCGCGGAGC CAACAGAACG AGGGTATTTC
    CCCCGTTTTG AGGAGCCAGT GCCCCTGTCC TATGAAGGCC GAGCGCACTA CGACCCACTG
    CCCAGGACCT CCACCTTGCG GCACGAAGAG CCTCCCGCCC CTGCCCCTGG GTACGACGTG
    CACGGAAGGT ACCGGCCTGA CGTGCAGCCC TACTCCACTG CTGGCCCCAA GGTGCCCCCT
    GAGCCCAAGC AGTACTTGGA CCAGTACCCT CGGAGTTACG AGCCAGCGCC AGCCCAGGGC
    TTCCCCTCCA GGGCAGGCCC AGGCCACTAC GAGCCTCTTC ACAGCACCAC GCTCGCTCCA
    CCACTTGGCC CCTCGTCCCA GCACAAGCCC GAAGTCCCGC CCACAGGCAC CAAACCCCTG
    CCTCCACCCC CAGCTCCGGC CGAGGAGGAG GAAGACCCAG CCATGAAGCC ACAATCCGTG
    CTCACCAGAG TGAAGATGTT TGAGAATAAG AGGTCTGCTT CCATGGAGAA CAGGAAGGAT
    GAGAGCCATT CCACTAGTTT CAAGCCCCCA GAAGTCACGT CAAAACCGGC AGGTGCTCCT
    GTCCCAGGTC CCAAAGCCCC TGCTCCGAGT CAGCTCGGTG AACACGACAG AGCAGCACAT
    AGGATCCCTG AACCACAGAG GCCTCACATG AAGCCACCTG AAGACATTGT TCGGGCAAAT
    CACTATGACC CCGAGGAGGA TGAGGAGTAC TACCGGAAGC AGCTCTCCTA CTTCGATCGG
    AGAAGCTTTG AGGCCAAGCC CCCCACCCAC ATCCCCACTA GCCACCTCTC CGAGCCCACC
    AGGCCGGCTC ACCCCCAATC TCAGCCACAG TTTGCCGGTT ACTCTTCCAA GGGGAAGGCT
    GCTGAGGCCG AAGCTGTGGA CAGGTCATTT GGGGAGAAGC GCTATGACCC GCTGCAGGCG
    ACGCCCCCCC CTCCGCCGCT GCCTGTTCAG TACAGCCAGG GTGCTCAACC CAGCCCCAAC
    ACGGCTCTTG GCCTCCACGC ACACACCAAG GGGGCGCATG GTGAAGGTAA TGCCATGTCG
    TTGGATTTTC AGAACTCGTT AGTGTCCAAA CCGGACCCAC CTCCATCTCA GAATAAGCCA
    GCAGCTTACA GACCAGCCCC TCGAGAGGAT GCTGTCCAGT CTGGCTTCTA CCCACAAAAG
    AGTTTCCCAG AGAAAGCCCC AGCTAATGGG GTCGAGCAGA CGCAGAAAAC CGTCACGCCA
    GCATACAATC GATTCACACC AAAACCATAC ACAAGCTCTG CACGGCCATT TGAACGCAAG
    TTTGAAAGCC CTAAATTCAA CCACAATCTC TTGCCGAATG AAACCGCACA CAAACCTGAC
    CTGTCCTCCA AAGCCCCCGC TTCTCCGAAA ACCCTCCTGA AGTCTGCACA GCCTCCTGAG
    TTTGACAGTG GCGTGGAGAC CCTCTCTGTC CACACTGACA ACAAGCTGAA ATACCAAGTA
    AACAGTGTTA GCATGGGGCC GAGAGCAGTG CCCGTGAGTC CCTCAGCCGT GGAGGAAGAT
    GAAGACGAAG ACGGTCACAC AGTGGTGGCT ACTGCCCGCG GCGTGTTTAA CAGCAATGGT
    GGGGTGCTGA GCTCCATTGA GACCGGTGTC AGCATCATCA TCCCCCAGGG AGCCATTCCC
    GAGGGGGTGG AACAGGAGAT CTACTTCAAG GTCTGCCGCG ACAACAGCAT CCTGCCGCCT
    TTGGACAAAG AGAAGGGTGA GACCCTGCTG AGCCCCCTAG TGATGTGTGG GCCCCATGGC
    CTCAAGTTCC TGAAGCCCGT GGAGCTGCGC CTACCACACT GTGCGTCCAT GACTCCTGAC
    GGTTGGTCTT TTGCTCTAAA ATCATCCGAC TCCTCGTCGG GTGATCCTAA AACCTGGCAA
    AACAAGTGTC TTCCTGGAGA TCCAAATTAT CTTGTTGGAG CAAACTGTGT TTCTGTCCTG
    ATTGACCACT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837779.1
    CDS2027..7261
    Translation

    Target ORF information:

    RefSeq Version XM_024982011.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 1 (TJP1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982011.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCCGCCA GGGCCGCGGC CGCCAAGGCT CCTGGATTTG GATTTGGAAT TGCAATATCT 
    GGTGGAAGAG ATAATCCACA TTTCCAGAGC GGGGAAACCT CCATCGTGAT TTCAGATGTG
    CTGAAGGGGG GGCCTGCGGA AGGACAGCTG CAGGAAAATG ACCGGGTGGC AATGGTTAAT
    GGAGTCTCCA TGGATAATGT TGAGCATGCG TTTGCTGTTC AGCAACTAAG GAAAAGTGGG
    AAAAATGCAA AAATAACCAT TCGAAGGAAG AAGAAAGTTC AGATCCCAGT AAATCGTCCT
    GATCCTGAGC CTGTGTCTGA GAACGAAGAG GACAGTTACG ACGAGGAAGC GCATGACCCG
    AGAAGTGGCC GTGGGGCCCT GGCAAACAGG AGGAGCGAGA AGAGCTGGGG CCGAGATAGG
    AGTGCCAGTC GGGAGAGGAG TCTGTCCCCG CGGTCTGACA GGCGGTCTGT AGCCTCCAGT
    CAGCCTGCTA GACCCACCAA AGTCACCCTG GTGAAGTCCC GGAAGAATGA AGAATATGGT
    CTTCGATTGG CCAGCCATAT ATTTGTGAAG GAAATTTCAC AAGATAGTTT GGCAGCAAGA
    GATGGCAATA TTCAAGAAGG CGATGTTGTA TTGAAGATAA ATGGTACCGT GACGGAAAAC
    ATGTCACTGA CAGATGCGAA GACGCTGATC GAGAGGTCTA AGGGCAAGTT AAAGATGGTG
    GTTCAGAGAG ATGAGCGGGC TACCCTGCTG AATGTCCCTG ACCTTTCTGA CAGCATCCAC
    TCTGCTGACG CCTCCGAGAG GGACGATATT TCAGAAATCC AGTCACTGGC ATCAGACCAT
    TCAGGTCGAT CCCACGACCG GCCTCCCCGT CACAGCCGCT CTCGGTCTCC TGACCAGCGG
    TCCGAGCCTT CTGACCATTC TAGACACTCT CCACAGCAGC CCAGCAGTGG CAGTCTCCGG
    AGCAGAGAGG AAGAGAGAAT TTCTAAACCA GGGGCTGTCT CGACTCCGGT AAAGCATGCA
    GATGATCATA CACCTAAAAC AGTGGAAGAA GTTGTAGTTG AGCGAAATGA GAAACAAGCA
    CCCAATCTTC CAGAACCAAA GCCTGTGTAC GCACAGGTAG GACAGCCAGA CGTGGATTTA
    CCTGTCAGTC CATCTGATGG CGTCCTGCCC AACTCAACTC ATGAAGATGG CATTCTTCGG
    CCCAGCATGA AGTTAGTAAA ATTCAGGAAA GGAGATAGTG TGGGTCTTCG ACTAGCTGGT
    GGAAACGATG TTGGCATATT TGTAGCTGGT GTTCTCGAAG ATAGCCCTGC TGCCAAAGAA
    GGCTTGGAGG AGGGCGACCA GATCCTCAGG GTAAACAATG TAGATTTCAC AAATATCATA
    AGAGAAGAAG CCGTCCTCTT CCTGCTTGAC CTCCCAAAAG GAGAAGAAGT GACCATATTG
    GCTCAGAAGA AGAAAGATGT TTATCGTCGC ATCGTAGAAT CCGATGTGGG TGATTCTTTC
    TATATCAGAA CCCATTTTGA ATATGAGAAG GAATCTCCCT ATGGACTCAG TTTTAACAAA
    GGAGAGGTGT TCCGTGTCGT GGACACTCTG TACAATGGGA AGCTGGGGTC CTGGCTTGCC
    ATTCGGATTG GCAAAAACCA TAAAGAGGTG GAACGTGGCA TCATCCCAAA CAAAAACAGA
    GCTGAGCAGT TAGCCAGTGT GCAGTATACA CTTCCCAAGA CAGCAGGGGG AGACCGCGCC
    GACTTCTGGA GGTTCCGCGG TCTGCGCAGC TCCAAGAGAA ACCTGCGCAA GAGCCGAGAA
    GACCTCTCAG CCCAGCCAGT CCAGACCAAG TTCCCGGCGT ATGAGAGGGT GGTTCTTCGA
    GAAGCTGGAT TCCTGAGGCC TGTAACCATC TTTGGACCAA TAGCTGATGT TGCCAGGGAG
    AAACTGGCAA GAGAGGAGCC AGATATTTAC CAGATCGCAA AAAGTGAACC TCGGGATGCA
    GGAACCGACC AGCGCAGCTC TGGCATTATT CGCCTCCACA CAATAAAGCA GATAATAGAT
    CAGGACAAAC ATGCCTTACT GGATGTAACG CCCAATGCAG TAGACCGTCT GAACTATGCC
    CAGTGGTATC CAATCGTGGT GTTCCTTAAC CCGGACTCAA AGCAGGGTGT GAAAACAATG
    CGAATGAGGT TATGTCCAGA ATCTCGGAAA AGCGCCAGGA AGTTATATGA GCGCTCTCAT
    AAACTTCGTA AAAATAATCA CCATCTTTTT ACAACTACAA TTAACTTAAA TTCAATGAAT
    GATGGCTGGT ATGGTGCACT GAAAGAAGCA ATCCAACAAC AACAGAACCA GTTGGTATGG
    GTTTCTGAGG GAAAGGCGGA TGGTGCTACA AGTGATGACC TTGATTTGCA TGATGATCGT
    CTGTCCTACC TGTCAGCCCC AGGTAGTGAA TACTCAATGT ATAGCACGGA CAGTAGACAC
    ACTTCTGACT ATGAAGACAC AGACACAGAA GGCGGGGCCT ACACTGATCA AGAACTAGAT
    GAGACCCTAA ATGATGAGGT GGGGACTCCC CCGGAGTCTG CCATTACCCG GTCCTCTGAG
    CCTGTCAGAG AGGACTCCTC TGGAATGCAT CCTGACCACC AGGCATATCC TCCCTACTCA
    CCACAAGCGC AGCCACAGCC AGGCCATAGA ATAGACTCCC CTGGATTTAA AACAGCCTCT
    CAACAGAAAG CAGAAGCCTC ATCTCCAGGC CCTTACCTTT CGCCTGAAAC AAACCCGGCA
    CCATCAGCCT CTGCTGTTAA TCCTCACGTA GACTTAACTA ATGTCAGACT GGAGGAGCCG
    AGCCCAGCTC CCCCCTCCTC TGACTCACCA CACGCCGACT CTTTACGAGC TCCAGGCACT
    GAGGCCGCTC ACGTGCAGCT CAAAGGTCCA GAGCCGTCCC TGTCGTCGCA TGTGGGCCCA
    GCAAAGGTGT ACAGGAAGGA CCCCTACCCT GAGGAGATGA TGAGACAGAA CCACGTCTTG
    AAGCAGCCGG CTGTGGTTCA CCCTGGGCAG AGGCCAGACA AGGAGCAAGG CCTGAGCTAC
    GAGCCGCAGC CTCCGTACAC AGAGCGACAG GCTGGCAGGG AGTTGGAGCG GCCTGCATAC
    AGATACGATG CCTCTAGCTA CCCGGAGCAG TTTGCTCGGA ACTATGACCA TCGTCTGCGC
    TATGACGACC GCCTCCTCAC CTATGAGGAA CAATGGCCCT ACTACGATGA CAAGCAGCCT
    TACCAGCCCC GGCCTCTTGA TGGTCAGCCT CCCGCGGAGC CAACAGAACG AGGGTATTTC
    CCCCGTTTTG AGGAGCCAGT GCCCCTGTCC TATGAAGGCC GAGCGCACTA CGACCCACTG
    CCCAGGACCT CCACCTTGCG GCACGAAGAG CCTCCCGCCC CTGCCCCTGG GTACGACGTG
    CACGGAAGGT ACCGGCCTGA CGTGCAGCCC TACTCCACTG CTGGCCCCAA GGTGCCCCCT
    GAGCCCAAGC AGTACTTGGA CCAGTACCCT CGGAGTTACG AGCCAGCGCC AGCCCAGGGC
    TTCCCCTCCA GGGCAGGCCC AGGCCACTAC GAGCCTCTTC ACAGCACCAC GCTCGCTCCA
    CCACTTGGCC CCTCGTCCCA GCACAAGCCC GAAGTCCCGC CCACAGGCAC CAAACCCCTG
    CCTCCACCCC CAGCTCCGGC CGAGGAGGAG GAAGACCCAG CCATGAAGCC ACAATCCGTG
    CTCACCAGAG TGAAGATGTT TGAGAATAAG AGGTCTGCTT CCATGGAGAA CAGGAAGGAT
    GAGAGCCATT CCACTAGTTT CAAGCCCCCA GAAGTCACGT CAAAACCGGC AGGTGCTCCT
    GTCCCAGGTC CCAAAGCCCC TGCTCCGAGT CAGCTCGGTG AACACGACAG AGCAGCACAT
    AGGATCCCTG AACCACAGAG GCCTCACATG AAGCCACCTG AAGACATTGT TCGGGCAAAT
    CACTATGACC CCGAGGAGGA TGAGGAGTAC TACCGGAAGC AGCTCTCCTA CTTCGATCGG
    AGAAGCTTTG AGGCCAAGCC CCCCACCCAC ATCCCCACTA GCCACCTCTC CGAGCCCACC
    AGGCCGGCTC ACCCCCAATC TCAGCCACAG TTTGCCGGTT ACTCTTCCAA GGGGAAGGCT
    GCTGAGGCCG AAGCTGTGGA CAGGTCATTT GGGGAGAAGC GCTATGACCC GCTGCAGGCG
    ACGCCCCCCC CTCCGCCGCT GCCTGTTCAG TACAGCCAGG GTGCTCAACC CAGCCCCAAC
    ACGGCTCTTG GCCTCCACGC ACACACCAAG GGGGCGCATG GTGAAGGTAA TGCCATGTCG
    TTGGATTTTC AGAACTCGTT AGTGTCCAAA CCGGACCCAC CTCCATCTCA GAATAAGCCA
    GCAGCTTACA GACCAGCCCC TCGAGAGGAT GCTGTCCAGT CTGGCTTCTA CCCACAAAAG
    AGTTTCCCAG AGAAAGCCCC AGCTAATGGG GTCGAGCAGA CGCAGAAAAC CGTCACGCCA
    GCATACAATC GATTCACACC AAAACCATAC ACAAGCTCTG CACGGCCATT TGAACGCAAG
    TTTGAAAGCC CTAAATTCAA CCACAATCTC TTGCCGAATG AAACCGCACA CAAACCTGAC
    CTGTCCTCCA AAGCCCCCGC TTCTCCGAAA ACCCTCCTGA AGTCTGCACA GCCTCCTGAG
    TTTGACAGTG GCGTGGAGAC CCTCTCTGTC CACACTGACA ACAAGCTGAA ATACCAAGTA
    AACAGTGTTA GCATGGGGCC GAGAGCAGTG CCCGTGAGTC CCTCAGCCGT GGAGGAAGAT
    GAAGACGAAG ACGGTCACAC AGTGGTGGCT ACTGCCCGCG GCGTGTTTAA CAGCAATGGT
    GGGGTGCTGA GCTCCATTGA GACCGGTGTC AGCATCATCA TCCCCCAGGG AGCCATTCCC
    GAGGGGGTGG AACAGGAGAT CTACTTCAAG GTCTGCCGCG ACAACAGCAT CCTGCCGCCT
    TTGGACAAAG AGAAGGGTGA GACCCTGCTG AGCCCCCTAG TGATGTGTGG GCCCCATGGC
    CTCAAGTTCC TGAAGCCCGT GGAGCTGCGC CTACCACACT GTGCGTCCAT GACTCCTGAC
    GGTTGGTCTT TTGCTCTAAA ATCATCCGAC TCCTCGTCGG GTGATCCTAA AACCTGGCAA
    AACAAGTGTC TTCCTGGAGA TCCAAATTAT CTTGTTGGAG CAAACTGTGT TTCTGTCCTG
    ATTGACCACT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228441
    Clone ID Related Accession (Same CDS sequence) XM_024982012.1
    Accession Version XM_024982012.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5232bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCCGCCA GGGCCGCGGC CGCCAAGAAC ACAACAATGG AGGAAACAGC TATATGGGAA 
    CAACACACGG TGACACTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGAAGAGATA ATCCACATTT CCAGAGCGGG GAAACCTCCA TCGTGATTTC AGATGTGCTG
    AAGGGGGGGC CTGCGGAAGG ACAGCTGCAG GAAAATGACC GGGTGGCAAT GGTTAATGGA
    GTCTCCATGG ATAATGTTGA GCATGCGTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TAACCATTCG AAGGAAGAAG AAAGTTCAGA TCCCAGTAAA TCGTCCTGAT
    CCTGAGCCTG TGTCTGAGAA CGAAGAGGAC AGTTACGACG AGGAAGCGCA TGACCCGAGA
    AGTGGCCGTG GGGCCCTGGC AAACAGGAGG AGCGAGAAGA GCTGGGGCCG AGATAGGAGT
    GCCAGTCGGG AGAGGAGTCT GTCCCCGCGG TCTGACAGGC GGTCTGTAGC CTCCAGTCAG
    CCTGCTAGAC CCACCAAAGT CACCCTGGTG AAGTCCCGGA AGAATGAAGA ATATGGTCTT
    CGATTGGCCA GCCATATATT TGTGAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGCGA TGTTGTATTG AAGATAAATG GTACCGTGAC GGAAAACATG
    TCACTGACAG ATGCGAAGAC GCTGATCGAG AGGTCTAAGG GCAAGTTAAA GATGGTGGTT
    CAGAGAGATG AGCGGGCTAC CCTGCTGAAT GTCCCTGACC TTTCTGACAG CATCCACTCT
    GCTGACGCCT CCGAGAGGGA CGATATTTCA GAAATCCAGT CACTGGCATC AGACCATTCA
    GGTCGATCCC ACGACCGGCC TCCCCGTCAC AGCCGCTCTC GGTCTCCTGA CCAGCGGTCC
    GAGCCTTCTG ACCATTCTAG ACACTCTCCA CAGCAGCCCA GCAGTGGCAG TCTCCGGAGC
    AGAGAGGAAG AGAGAATTTC TAAACCAGGG GCTGTCTCGA CTCCGGTAAA GCATGCAGAT
    GATCATACAC CTAAAACAGT GGAAGAAGTT GTAGTTGAGC GAAATGAGAA ACAAGCACCC
    AATCTTCCAG AACCAAAGCC TGTGTACGCA CAGGTAGGAC AGCCAGACGT GGATTTACCT
    GTCAGTCCAT CTGATGGCGT CCTGCCCAAC TCAACTCATG AAGATGGCAT TCTTCGGCCC
    AGCATGAAGT TAGTAAAATT CAGGAAAGGA GATAGTGTGG GTCTTCGACT AGCTGGTGGA
    AACGATGTTG GCATATTTGT AGCTGGTGTT CTCGAAGATA GCCCTGCTGC CAAAGAAGGC
    TTGGAGGAGG GCGACCAGAT CCTCAGGGTA AACAATGTAG ATTTCACAAA TATCATAAGA
    GAAGAAGCCG TCCTCTTCCT GCTTGACCTC CCAAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AAGATGTTTA TCGTCGCATC GTAGAATCCG ATGTGGGTGA TTCTTTCTAT
    ATCAGAACCC ATTTTGAATA TGAGAAGGAA TCTCCCTATG GACTCAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTCGTGGA CACTCTGTAC AATGGGAAGC TGGGGTCCTG GCTTGCCATT
    CGGATTGGCA AAAACCATAA AGAGGTGGAA CGTGGCATCA TCCCAAACAA AAACAGAGCT
    GAGCAGTTAG CCAGTGTGCA GTATACACTT CCCAAGACAG CAGGGGGAGA CCGCGCCGAC
    TTCTGGAGGT TCCGCGGTCT GCGCAGCTCC AAGAGAAACC TGCGCAAGAG CCGAGAAGAC
    CTCTCAGCCC AGCCAGTCCA GACCAAGTTC CCGGCGTATG AGAGGGTGGT TCTTCGAGAA
    GCTGGATTCC TGAGGCCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGGGAGAAA
    CTGGCAAGAG AGGAGCCAGA TATTTACCAG ATCGCAAAAA GTGAACCTCG GGATGCAGGA
    ACCGACCAGC GCAGCTCTGG CATTATTCGC CTCCACACAA TAAAGCAGAT AATAGATCAG
    GACAAACATG CCTTACTGGA TGTAACGCCC AATGCAGTAG ACCGTCTGAA CTATGCCCAG
    TGGTATCCAA TCGTGGTGTT CCTTAACCCG GACTCAAAGC AGGGTGTGAA AACAATGCGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGC GCCAGGAAGT TATATGAGCG CTCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGCTGGTATG GTGCACTGAA AGAAGCAATC CAACAACAAC AGAACCAGTT GGTATGGGTT
    TCTGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCCCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAG
    ACCCTAAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACCCGGTC CTCTGAGCCT
    GTCAGAGAGG ACTCCTCTGG AATGCATCCT GACCACCAGG CATATCCTCC CTACTCACCA
    CAAGCGCAGC CACAGCCAGG CCATAGAATA GACTCCCCTG GATTTAAAAC AGCCTCTCAA
    CAGAAAGCAG AAGCCTCATC TCCAGGCCCT TACCTTTCGC CTGAAACAAA CCCGGCACCA
    TCAGCCTCTG CTGTTAATCC TCACGTAGAC TTAACTAATG TCAGACTGGA GGAGCCGAGC
    CCAGCTCCCC CCTCCTCTGA CTCACCACAC GCCGACTCTT TACGAGCTCC AGGCACTGAG
    GCCGCTCACG TGCAGCTCAA AGGTCCAGAG CCGTCCCTGT CGTCGCATGT GGGCCCAGCA
    AAGGTGTACA GGAAGGACCC CTACCCTGAG GAGATGATGA GACAGAACCA CGTCTTGAAG
    CAGCCGGCTG TGGTTCACCC TGGGCAGAGG CCAGACAAGG AGCAAGGCCT GAGCTACGAG
    CCGCAGCCTC CGTACACAGA GCGACAGGCT GGCAGGGAGT TGGAGCGGCC TGCATACAGA
    TACGATGCCT CTAGCTACCC GGAGCAGTTT GCTCGGAACT ATGACCATCG TCTGCGCTAT
    GACGACCGCC TCCTCACCTA TGAGGAACAA TGGCCCTACT ACGATGACAA GCAGCCTTAC
    CAGCCCCGGC CTCTTGATGG TCAGCCTCCC GCGGAGCCAA CAGAACGAGG GTATTTCCCC
    CGTTTTGAGG AGCCAGTGCC CCTGTCCTAT GAAGGCCGAG CGCACTACGA CCCACTGCCC
    AGGACCTCCA CCTTGCGGCA CGAAGAGCCT CCCGCCCCTG CCCCTGGGTA CGACGTGCAC
    GGAAGGTACC GGCCTGACGT GCAGCCCTAC TCCACTGCTG GCCCCAAGGT GCCCCCTGAG
    CCCAAGCAGT ACTTGGACCA GTACCCTCGG AGTTACGAGC CAGCGCCAGC CCAGGGCTTC
    CCCTCCAGGG CAGGCCCAGG CCACTACGAG CCTCTTCACA GCACCACGCT CGCTCCACCA
    CTTGGCCCCT CGTCCCAGCA CAAGCCCGAA GTCCCGCCCA CAGGCACCAA ACCCCTGCCT
    CCACCCCCAG CTCCGGCCGA GGAGGAGGAA GACCCAGCCA TGAAGCCACA ATCCGTGCTC
    ACCAGAGTGA AGATGTTTGA GAATAAGAGG TCTGCTTCCA TGGAGAACAG GAAGGATGAG
    AGCCATTCCA CTAGTTTCAA GCCCCCAGAA GTCACGTCAA AACCGGCAGG TGCTCCTGTC
    CCAGGTCCCA AAGCCCCTGC TCCGAGTCAG CTCGGTGAAC ACGACAGAGC AGCACATAGG
    ATCCCTGAAC CACAGAGGCC TCACATGAAG CCACCTGAAG ACATTGTTCG GGCAAATCAC
    TATGACCCCG AGGAGGATGA GGAGTACTAC CGGAAGCAGC TCTCCTACTT CGATCGGAGA
    AGCTTTGAGG CCAAGCCCCC CACCCACATC CCCACTAGCC ACCTCTCCGA GCCCACCAGG
    CCGGCTCACC CCCAATCTCA GCCACAGTTT GCCGGTTACT CTTCCAAGGG GAAGGCTGCT
    GAGGCCGAAG CTGTGGACAG GTCATTTGGG GAGAAGCGCT ATGACCCGCT GCAGGCGACG
    CCCCCCCCTC CGCCGCTGCC TGTTCAGTAC AGCCAGGGTG CTCAACCCAG CCCCAACACG
    GCTCTTGGCC TCCACGCACA CACCAAGGGG GCGCATGGTG AAGGTAATGC CATGTCGTTG
    GATTTTCAGA ACTCGTTAGT GTCCAAACCG GACCCACCTC CATCTCAGAA TAAGCCAGCA
    GCTTACAGAC CAGCCCCTCG AGAGGATGCT GTCCAGTCTG GCTTCTACCC ACAAAAGAGT
    TTCCCAGAGA AAGCCCCAGC TAATGGGGTC GAGCAGACGC AGAAAACCGT CACGCCAGCA
    TACAATCGAT TCACACCAAA ACCATACACA AGCTCTGCAC GGCCATTTGA ACGCAAGTTT
    GAAAGCCCTA AATTCAACCA CAATCTCTTG CCGAATGAAA CCGCACACAA ACCTGACCTG
    TCCTCCAAAG CCCCCGCTTC TCCGAAAACC CTCCTGAAGT CTGCACAGCC TCCTGAGTTT
    GACAGTGGCG TGGAGACCCT CTCTGTCCAC ACTGACAACA AGCTGAAATA CCAAGTAAAC
    AGTGTTAGCA TGGGGCCGAG AGCAGTGCCC GTGAGTCCCT CAGCCGTGGA GGAAGATGAA
    GACGAAGACG GTCACACAGT GGTGGCTACT GCCCGCGGCG TGTTTAACAG CAATGGTGGG
    GTGCTGAGCT CCATTGAGAC CGGTGTCAGC ATCATCATCC CCCAGGGAGC CATTCCCGAG
    GGGGTGGAAC AGGAGATCTA CTTCAAGGTC TGCCGCGACA ACAGCATCCT GCCGCCTTTG
    GACAAAGAGA AGGGTGAGAC CCTGCTGAGC CCCCTAGTGA TGTGTGGGCC CCATGGCCTC
    AAGTTCCTGA AGCCCGTGGA GCTGCGCCTA CCACACTGTG ATCCTAAAAC CTGGCAAAAC
    AAGTGTCTTC CTGGAGATCC AAATTATCTT GTTGGAGCAA ACTGTGTTTC TGTCCTGATT
    GACCACTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837780.1
    CDS2027..7258
    Translation

    Target ORF information:

    RefSeq Version XM_024982012.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 1 (TJP1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982012.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCCGCCA GGGCCGCGGC CGCCAAGAAC ACAACAATGG AGGAAACAGC TATATGGGAA 
    CAACACACGG TGACACTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGAAGAGATA ATCCACATTT CCAGAGCGGG GAAACCTCCA TCGTGATTTC AGATGTGCTG
    AAGGGGGGGC CTGCGGAAGG ACAGCTGCAG GAAAATGACC GGGTGGCAAT GGTTAATGGA
    GTCTCCATGG ATAATGTTGA GCATGCGTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TAACCATTCG AAGGAAGAAG AAAGTTCAGA TCCCAGTAAA TCGTCCTGAT
    CCTGAGCCTG TGTCTGAGAA CGAAGAGGAC AGTTACGACG AGGAAGCGCA TGACCCGAGA
    AGTGGCCGTG GGGCCCTGGC AAACAGGAGG AGCGAGAAGA GCTGGGGCCG AGATAGGAGT
    GCCAGTCGGG AGAGGAGTCT GTCCCCGCGG TCTGACAGGC GGTCTGTAGC CTCCAGTCAG
    CCTGCTAGAC CCACCAAAGT CACCCTGGTG AAGTCCCGGA AGAATGAAGA ATATGGTCTT
    CGATTGGCCA GCCATATATT TGTGAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGCGA TGTTGTATTG AAGATAAATG GTACCGTGAC GGAAAACATG
    TCACTGACAG ATGCGAAGAC GCTGATCGAG AGGTCTAAGG GCAAGTTAAA GATGGTGGTT
    CAGAGAGATG AGCGGGCTAC CCTGCTGAAT GTCCCTGACC TTTCTGACAG CATCCACTCT
    GCTGACGCCT CCGAGAGGGA CGATATTTCA GAAATCCAGT CACTGGCATC AGACCATTCA
    GGTCGATCCC ACGACCGGCC TCCCCGTCAC AGCCGCTCTC GGTCTCCTGA CCAGCGGTCC
    GAGCCTTCTG ACCATTCTAG ACACTCTCCA CAGCAGCCCA GCAGTGGCAG TCTCCGGAGC
    AGAGAGGAAG AGAGAATTTC TAAACCAGGG GCTGTCTCGA CTCCGGTAAA GCATGCAGAT
    GATCATACAC CTAAAACAGT GGAAGAAGTT GTAGTTGAGC GAAATGAGAA ACAAGCACCC
    AATCTTCCAG AACCAAAGCC TGTGTACGCA CAGGTAGGAC AGCCAGACGT GGATTTACCT
    GTCAGTCCAT CTGATGGCGT CCTGCCCAAC TCAACTCATG AAGATGGCAT TCTTCGGCCC
    AGCATGAAGT TAGTAAAATT CAGGAAAGGA GATAGTGTGG GTCTTCGACT AGCTGGTGGA
    AACGATGTTG GCATATTTGT AGCTGGTGTT CTCGAAGATA GCCCTGCTGC CAAAGAAGGC
    TTGGAGGAGG GCGACCAGAT CCTCAGGGTA AACAATGTAG ATTTCACAAA TATCATAAGA
    GAAGAAGCCG TCCTCTTCCT GCTTGACCTC CCAAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AAGATGTTTA TCGTCGCATC GTAGAATCCG ATGTGGGTGA TTCTTTCTAT
    ATCAGAACCC ATTTTGAATA TGAGAAGGAA TCTCCCTATG GACTCAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTCGTGGA CACTCTGTAC AATGGGAAGC TGGGGTCCTG GCTTGCCATT
    CGGATTGGCA AAAACCATAA AGAGGTGGAA CGTGGCATCA TCCCAAACAA AAACAGAGCT
    GAGCAGTTAG CCAGTGTGCA GTATACACTT CCCAAGACAG CAGGGGGAGA CCGCGCCGAC
    TTCTGGAGGT TCCGCGGTCT GCGCAGCTCC AAGAGAAACC TGCGCAAGAG CCGAGAAGAC
    CTCTCAGCCC AGCCAGTCCA GACCAAGTTC CCGGCGTATG AGAGGGTGGT TCTTCGAGAA
    GCTGGATTCC TGAGGCCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGGGAGAAA
    CTGGCAAGAG AGGAGCCAGA TATTTACCAG ATCGCAAAAA GTGAACCTCG GGATGCAGGA
    ACCGACCAGC GCAGCTCTGG CATTATTCGC CTCCACACAA TAAAGCAGAT AATAGATCAG
    GACAAACATG CCTTACTGGA TGTAACGCCC AATGCAGTAG ACCGTCTGAA CTATGCCCAG
    TGGTATCCAA TCGTGGTGTT CCTTAACCCG GACTCAAAGC AGGGTGTGAA AACAATGCGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGC GCCAGGAAGT TATATGAGCG CTCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGCTGGTATG GTGCACTGAA AGAAGCAATC CAACAACAAC AGAACCAGTT GGTATGGGTT
    TCTGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCCCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAG
    ACCCTAAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACCCGGTC CTCTGAGCCT
    GTCAGAGAGG ACTCCTCTGG AATGCATCCT GACCACCAGG CATATCCTCC CTACTCACCA
    CAAGCGCAGC CACAGCCAGG CCATAGAATA GACTCCCCTG GATTTAAAAC AGCCTCTCAA
    CAGAAAGCAG AAGCCTCATC TCCAGGCCCT TACCTTTCGC CTGAAACAAA CCCGGCACCA
    TCAGCCTCTG CTGTTAATCC TCACGTAGAC TTAACTAATG TCAGACTGGA GGAGCCGAGC
    CCAGCTCCCC CCTCCTCTGA CTCACCACAC GCCGACTCTT TACGAGCTCC AGGCACTGAG
    GCCGCTCACG TGCAGCTCAA AGGTCCAGAG CCGTCCCTGT CGTCGCATGT GGGCCCAGCA
    AAGGTGTACA GGAAGGACCC CTACCCTGAG GAGATGATGA GACAGAACCA CGTCTTGAAG
    CAGCCGGCTG TGGTTCACCC TGGGCAGAGG CCAGACAAGG AGCAAGGCCT GAGCTACGAG
    CCGCAGCCTC CGTACACAGA GCGACAGGCT GGCAGGGAGT TGGAGCGGCC TGCATACAGA
    TACGATGCCT CTAGCTACCC GGAGCAGTTT GCTCGGAACT ATGACCATCG TCTGCGCTAT
    GACGACCGCC TCCTCACCTA TGAGGAACAA TGGCCCTACT ACGATGACAA GCAGCCTTAC
    CAGCCCCGGC CTCTTGATGG TCAGCCTCCC GCGGAGCCAA CAGAACGAGG GTATTTCCCC
    CGTTTTGAGG AGCCAGTGCC CCTGTCCTAT GAAGGCCGAG CGCACTACGA CCCACTGCCC
    AGGACCTCCA CCTTGCGGCA CGAAGAGCCT CCCGCCCCTG CCCCTGGGTA CGACGTGCAC
    GGAAGGTACC GGCCTGACGT GCAGCCCTAC TCCACTGCTG GCCCCAAGGT GCCCCCTGAG
    CCCAAGCAGT ACTTGGACCA GTACCCTCGG AGTTACGAGC CAGCGCCAGC CCAGGGCTTC
    CCCTCCAGGG CAGGCCCAGG CCACTACGAG CCTCTTCACA GCACCACGCT CGCTCCACCA
    CTTGGCCCCT CGTCCCAGCA CAAGCCCGAA GTCCCGCCCA CAGGCACCAA ACCCCTGCCT
    CCACCCCCAG CTCCGGCCGA GGAGGAGGAA GACCCAGCCA TGAAGCCACA ATCCGTGCTC
    ACCAGAGTGA AGATGTTTGA GAATAAGAGG TCTGCTTCCA TGGAGAACAG GAAGGATGAG
    AGCCATTCCA CTAGTTTCAA GCCCCCAGAA GTCACGTCAA AACCGGCAGG TGCTCCTGTC
    CCAGGTCCCA AAGCCCCTGC TCCGAGTCAG CTCGGTGAAC ACGACAGAGC AGCACATAGG
    ATCCCTGAAC CACAGAGGCC TCACATGAAG CCACCTGAAG ACATTGTTCG GGCAAATCAC
    TATGACCCCG AGGAGGATGA GGAGTACTAC CGGAAGCAGC TCTCCTACTT CGATCGGAGA
    AGCTTTGAGG CCAAGCCCCC CACCCACATC CCCACTAGCC ACCTCTCCGA GCCCACCAGG
    CCGGCTCACC CCCAATCTCA GCCACAGTTT GCCGGTTACT CTTCCAAGGG GAAGGCTGCT
    GAGGCCGAAG CTGTGGACAG GTCATTTGGG GAGAAGCGCT ATGACCCGCT GCAGGCGACG
    CCCCCCCCTC CGCCGCTGCC TGTTCAGTAC AGCCAGGGTG CTCAACCCAG CCCCAACACG
    GCTCTTGGCC TCCACGCACA CACCAAGGGG GCGCATGGTG AAGGTAATGC CATGTCGTTG
    GATTTTCAGA ACTCGTTAGT GTCCAAACCG GACCCACCTC CATCTCAGAA TAAGCCAGCA
    GCTTACAGAC CAGCCCCTCG AGAGGATGCT GTCCAGTCTG GCTTCTACCC ACAAAAGAGT
    TTCCCAGAGA AAGCCCCAGC TAATGGGGTC GAGCAGACGC AGAAAACCGT CACGCCAGCA
    TACAATCGAT TCACACCAAA ACCATACACA AGCTCTGCAC GGCCATTTGA ACGCAAGTTT
    GAAAGCCCTA AATTCAACCA CAATCTCTTG CCGAATGAAA CCGCACACAA ACCTGACCTG
    TCCTCCAAAG CCCCCGCTTC TCCGAAAACC CTCCTGAAGT CTGCACAGCC TCCTGAGTTT
    GACAGTGGCG TGGAGACCCT CTCTGTCCAC ACTGACAACA AGCTGAAATA CCAAGTAAAC
    AGTGTTAGCA TGGGGCCGAG AGCAGTGCCC GTGAGTCCCT CAGCCGTGGA GGAAGATGAA
    GACGAAGACG GTCACACAGT GGTGGCTACT GCCCGCGGCG TGTTTAACAG CAATGGTGGG
    GTGCTGAGCT CCATTGAGAC CGGTGTCAGC ATCATCATCC CCCAGGGAGC CATTCCCGAG
    GGGGTGGAAC AGGAGATCTA CTTCAAGGTC TGCCGCGACA ACAGCATCCT GCCGCCTTTG
    GACAAAGAGA AGGGTGAGAC CCTGCTGAGC CCCCTAGTGA TGTGTGGGCC CCATGGCCTC
    AAGTTCCTGA AGCCCGTGGA GCTGCGCCTA CCACACTGTG ATCCTAAAAC CTGGCAAAAC
    AAGTGTCTTC CTGGAGATCC AAATTATCTT GTTGGAGCAA ACTGTGTTTC TGTCCTGATT
    GACCACTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45684
    Clone ID Related Accession (Same CDS sequence) XM_015468497.1 , XM_010817146.1
    Accession Version XM_015468497.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5118bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646534.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAAGTACC AGAAGTACCT GACGGCGCTG CAGATGGCCA TCGGCGTCAC GCCGACCAAC 
    CGCGGCAGCC TGCTGCCGCT CAAGAGGAGG CTGTGGGTAA CGCCGTCCTC TGAAAACCCC
    AGTGGTGCTA CTTCTAGTGT CAGCCAAGGA AAGCCTTCTT TAAGACGAAT TAAAGGGAGA
    CTGCACAGGA GCAAGAGCCT CGATAGCATC GATTTCTGTG AACTCACTAA CACAACAATG
    GAGGAAACAG CTATATGGGA ACAACACACG GTGACACTTC ACAGGGCTCC TGGATTTGGA
    TTTGGAATTG CAATATCTGG TGGAAGAGAT AATCCACATT TCCAGAGCGG GGAAACCTCC
    ATCGTGATTT CAGATGTGCT GAAGGGGGGG CCTGCGGAAG GACAGCTGCA GGAAAATGAC
    CGGGTGGCAA TGGTTAATGG AGTCTCCATG GATAATGTTG AGCATGCGTT TGCTGTTCAG
    CAACTAAGGA AAAGTGGGAA AAATGCAAAA ATAACCATTC GAAGGAAGAA GAAAGTTCAG
    ATCCCAGTAA ATCGTCCTGA TCCTGAGCCT GTGTCTGAGA ACGAAGAGGA CAGTTACGAC
    GAGGAAGCGC ATGACCCGAG AAGTGGCCGT GGGGCCCTGG CAAACAGGAG GAGCGAGAAG
    AGCTGGGGCC GAGATAGGAG TGCCAGTCGG GAGAGGAGTC TGTCCCCGCG GTCTGACAGG
    CGGTCTGTAG CCTCCAGTCA GCCTGCTAGA CCCACCAAAG TCACCCTGGT GAAGTCCCGG
    AAGAATGAAG GTATTTTCTG CCAATCTAGC CTTTTCACAG TTTTACTAAT GTACAGTCTC
    CGGAGCAGAG AGGAAGAGAG AATTTCTAAA CCAGGGGCTG TCTCGACTCC GGTAAAGCAT
    GCAGATGATC ATACACCTAA AACAGTGGAA GAAGTTGTAG TTGAGCGAAA TGAGAAACAA
    GCACCCAATC TTCCAGAACC AAAGCCTGTG TACGCACAGG TAGGACAGCC AGACGTGGAT
    TTACCTGTCA GTCCATCTGA TGGCGTCCTG CCCAACTCAA CTCATGAAGA TGGCATTCTT
    CGGCCCAGCA TGAAGTTAGT AAAATTCAGG AAAGGAGATA GTGTGGGTCT TCGACTAGCT
    GGTGGAAACG ATGTTGGCAT ATTTGTAGCT GGTGTTCTCG AAGATAGCCC TGCTGCCAAA
    GAAGGCTTGG AGGAGGGCGA CCAGATCCTC AGGGTAAACA ATGTAGATTT CACAAATATC
    ATAAGAGAAG AAGCCGTCCT CTTCCTGCTT GACCTCCCAA AAGGAGAAGA AGTGACCATA
    TTGGCTCAGA AGAAGAAAGA TGTTTATCGT CGCATCGTAG AATCCGATGT GGGTGATTCT
    TTCTATATCA GAACCCATTT TGAATATGAG AAGGAATCTC CCTATGGACT CAGTTTTAAC
    AAAGGAGAGG TGTTCCGTGT CGTGGACACT CTGTACAATG GGAAGCTGGG GTCCTGGCTT
    GCCATTCGGA TTGGCAAAAA CCATAAAGAG GTGGAACGTG GCATCATCCC AAACAAAAAC
    AGAGCTGAGC AGTTAGCCAG TGTGCAGTAT ACACTTCCCA AGACAGCAGG GGGAGACCGC
    GCCGACTTCT GGAGGTTCCG CGGTCTGCGC AGCTCCAAGA GAAACCTGCG CAAGAGCCGA
    GAAGACCTCT CAGCCCAGCC AGTCCAGACC AAGTTCCCGG CGTATGAGAG GGTGGTTCTT
    CGAGAAGCTG GATTCCTGAG GCCTGTAACC ATCTTTGGAC CAATAGCTGA TGTTGCCAGG
    GAGAAACTGG CAAGAGAGGA GCCAGATATT TACCAGATCG CAAAAAGTGA ACCTCGGGAT
    GCAGGAACCG ACCAGCGCAG CTCTGGCATT ATTCGCCTCC ACACAATAAA GCAGATAATA
    GATCAGGACA AACATGCCTT ACTGGATGTA ACGCCCAATG CAGTAGACCG TCTGAACTAT
    GCCCAGTGGT ATCCAATCGT GGTGTTCCTT AACCCGGACT CAAAGCAGGG TGTGAAAACA
    ATGCGAATGA GGTTATGTCC AGAATCTCGG AAAAGCGCCA GGAAGTTATA TGAGCGCTCT
    CATAAACTTC GTAAAAATAA TCACCATCTT TTTACAACTA CAATTAACTT AAATTCAATG
    AATGATGGCT GGTATGGTGC ACTGAAAGAA GCAATCCAAC AACAACAGAA CCAGTTGGTA
    TGGGTTTCTG AGGGAAAGGC GGATGGTGCT ACAAGTGATG ACCTTGATTT GCATGATGAT
    CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT GAATACTCAA TGTATAGCAC GGACAGTAGA
    CACACTTCTG ACTATGAAGA CACAGACACA GAAGGCGGGG CCTACACTGA TCAAGAACTA
    GATGAGACCC TAAATGATGA GGTGGGGACT CCCCCGGAGT CTGCCATTAC CCGGTCCTCT
    GAGCCTGTCA GAGAGGACTC CTCTGGAATG CATCCTGACC ACCAGGCATA TCCTCCCTAC
    TCACCACAAG CGCAGCCACA GCCAGGCCAT AGAATAGACT CCCCTGGATT TAAAACAGCC
    TCTCAACAGA AAGCAGAAGC CTCATCTCCA GGCCCTTACC TTTCGCCTGA AACAAACCCG
    GCACCATCAG CCTCTGCTGT TAATCCTCAC GTAGACTTAA CTAATGTCAG ACTGGAGGAG
    CCGAGCCCAG CTCCCCCCTC CTCTGACTCA CCACACGCCG ACTCTTTACG AGCTCCAGGC
    ACTGAGGCCG CTCACGTGCA GCTCAAAGGT CCAGAGCCGT CCCTGTCGTC GCATGTGGGC
    CCAGCAAAGG TGTACAGGAA GGACCCCTAC CCTGAGGAGA TGATGAGACA GAACCACGTC
    TTGAAGCAGC CGGCTGTGGT TCACCCTGGG CAGAGGCCAG ACAAGGAGCA AGGCCTGAGC
    TACGAGCCGC AGCCTCCGTA CACAGAGCGA CAGGCTGGCA GGGAGTTGGA GCAGCCTGCA
    TACAGATACG ATGCCTCTAG CTACCCGGAG CAGTTTGCTC GGAACTATGA CCATCGTCTG
    CGCTATGACG ACCGCCTCCT CACCTATGAG GAACAATGGC CCTACTACGA TGACAAGCAG
    CCTTACCAGC CCCGGCCTCT TGATGGTCAG CCTCCCGCGG AGCCAACAGA ACGAGGGTAT
    TTCCCCCGTT TTGAGGAGCC AGTGCCCCTG TCCTATGAAG GCCGAGCGCA CTACGACCCA
    CTGCCCAGGA CCTCCACCTT GCGGCACGAA GAGCCTCCCG CCCCTGCCCC TGGGTACGAC
    GTGCACGGAA GGTACCGGCC TGACGTGCAG CCCTACTCCA CTGCTGGCCC CAAGGTGCCC
    CCTGAGCCCA AGCAGTACTT GGACCAGTAC CCTCGGAGTT ACGAGCCAGC GCCAGCCCAG
    GGCTTCCCCT CCAGGGCAGG CCCAGGCCAC TACGAGCCTC TTCACAGCAC CACGCTCGCT
    CCACCACTTG GCCCCTCGTC CCAGCACAAG CCCGAAGTCC CGCCCACAGG CACCAAACCC
    CTGCCTCCAC CCCCAGCTCC GGCCGAGGAG GAGGAAGACC CAGCCATGAA GCCACAATCC
    GTGCTCACCA GAGTGAAGAT GTTTGAGAAT AAGAGGTCTG CTTCCATGGA GAACAGGAAG
    GATGAGAGCC ATTCCACTAG TTTCAAGCCC CCAGAAGTCA CGTCAAAACC GGCAGGTGCT
    CCTGTCCCAG GTCCCAAAGC CCCTGCTCCG AGTCAGCTCG GTGAACACGA CAGAGCAGCA
    CATAGGATCC CTGAACCACA GAGGCCTCAC ATGAAGCCAC CTGAAGACAT TGTTCGGGCA
    AATCACTATG ACCCCGAGGA GGATGAGGAG TACTACCGGA AGCAGCTCTC CTACTTCGAT
    CGGAGAAGCT TTGAGGCCAA GCCCCCCACC CACATCCCCA CTAGCCACCT CTCCGAGCCC
    ACCAGGCCGG CTCACCCCCA ATCTCAGCCA CAGTTTGCCG GTTACTCTTC CAAGGGGAAG
    GCTGCTGAGG CCGAAGCTGT GGACAGGTCA TTTGGGGAGA AGCGCTATGA CCCGCTGCAG
    GCGACGCCCC CCCCTCCGCC GCTGCCTGTT CAGTACAGCC AGGGTGCTCA ACCCAGCCCC
    AACACGGCTC TTGGCCTCCA CGCACACACC AAGGGGGCGC ATGGTGAAGG TAATGCCATG
    TCGTTGGATT TTCAGAACTC GTTAGTGTCC AAACCGGACC CACCTCCATC TCAGAATAAG
    CCAGCAGCTT ACAGACCAGC CCCTCGAGAG GATGCTGTCC AGTCTGGCTT CTACCCACAA
    AAGAGTTTCC CAGAGAAAGC CCCAGCTAAT GGGGTCGAGC AGACGCAGAA AACCGTCACG
    CCAGCATACA ATCGATTCAC ACCAAAACCA TACACAAGCT CTGCACGGCC ATTTGAACGC
    AAGTTTGAAA GCCCTAAATT CAACCACAAT CTCTTGCCGA ATGAAACCGC ACACAAACCT
    GACCTGTCCT CCAAAGCCCC CGCTTCTCCG AAAACCCTCC TGAAGTCTGC ACAGCCTCCT
    GAGTTTGACA GTGGCGTGGA GACCCTCTCT GTCCACACTG ACAACAAGCT GAAATACCAA
    GTAAACAGTG TTAGCATGGG GCCGAGAGCA GTGCCCGTGA GTCCCTCAGC CGTGGAGGAA
    GATGAAGACG AAGACGGTCA CACAGTGGTG GCTACTGCCC GCGGCGTGTT TAACAGCAAT
    GGTGGGGTGC TGAGCTCCAT TGAGACCGGT GTCAGCATCA TCATCCCCCA GGGAGCCATT
    CCCGAGGGGG TGGAACAGGA GATCTACTTC AAGGTCTGCC GCGACAACAG CATCCTGCCG
    CCTTTGGACA AAGAGAAGGG TGAGACCCTG CTGAGCCCCC TAGTGATGTG TGGGCCCCAT
    GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCCTACCAC ACTGTGCGTC CATGACTCCT
    GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGATCC TAAAACCTGG
    CAAAACAAGT GTCTTCCTGG AGATCCAAAT TATCTTGTTG GAGCAAACTG TGTTTCTGTC
    CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323983.1
    CDS16..5133
    Translation

    Target ORF information:

    RefSeq Version XM_015468497.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 1 (TJP1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468497.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAAGTACC AGAAGTACCT GACGGCGCTG CAGATGGCCA TCGGCGTCAC GCCGACCAAC 
    CGCGGCAGCC TGCTGCCGCT CAAGAGGAGG CTGTGGGTAA CGCCGTCCTC TGAAAACCCC
    AGTGGTGCTA CTTCTAGTGT CAGCCAAGGA AAGCCTTCTT TAAGACGAAT TAAAGGGAGA
    CTGCACAGGA GCAAGAGCCT CGATAGCATC GATTTCTGTG AACTCACTAA CACAACAATG
    GAGGAAACAG CTATATGGGA ACAACACACG GTGACACTTC ACAGGGCTCC TGGATTTGGA
    TTTGGAATTG CAATATCTGG TGGAAGAGAT AATCCACATT TCCAGAGCGG GGAAACCTCC
    ATCGTGATTT CAGATGTGCT GAAGGGGGGG CCTGCGGAAG GACAGCTGCA GGAAAATGAC
    CGGGTGGCAA TGGTTAATGG AGTCTCCATG GATAATGTTG AGCATGCGTT TGCTGTTCAG
    CAACTAAGGA AAAGTGGGAA AAATGCAAAA ATAACCATTC GAAGGAAGAA GAAAGTTCAG
    ATCCCAGTAA ATCGTCCTGA TCCTGAGCCT GTGTCTGAGA ACGAAGAGGA CAGTTACGAC
    GAGGAAGCGC ATGACCCGAG AAGTGGCCGT GGGGCCCTGG CAAACAGGAG GAGCGAGAAG
    AGCTGGGGCC GAGATAGGAG TGCCAGTCGG GAGAGGAGTC TGTCCCCGCG GTCTGACAGG
    CGGTCTGTAG CCTCCAGTCA GCCTGCTAGA CCCACCAAAG TCACCCTGGT GAAGTCCCGG
    AAGAATGAAG GTATTTTCTG CCAATCTAGC CTTTTCACAG TTTTACTAAT GTACAGTCTC
    CGGAGCAGAG AGGAAGAGAG AATTTCTAAA CCAGGGGCTG TCTCGACTCC GGTAAAGCAT
    GCAGATGATC ATACACCTAA AACAGTGGAA GAAGTTGTAG TTGAGCGAAA TGAGAAACAA
    GCACCCAATC TTCCAGAACC AAAGCCTGTG TACGCACAGG TAGGACAGCC AGACGTGGAT
    TTACCTGTCA GTCCATCTGA TGGCGTCCTG CCCAACTCAA CTCATGAAGA TGGCATTCTT
    CGGCCCAGCA TGAAGTTAGT AAAATTCAGG AAAGGAGATA GTGTGGGTCT TCGACTAGCT
    GGTGGAAACG ATGTTGGCAT ATTTGTAGCT GGTGTTCTCG AAGATAGCCC TGCTGCCAAA
    GAAGGCTTGG AGGAGGGCGA CCAGATCCTC AGGGTAAACA ATGTAGATTT CACAAATATC
    ATAAGAGAAG AAGCCGTCCT CTTCCTGCTT GACCTCCCAA AAGGAGAAGA AGTGACCATA
    TTGGCTCAGA AGAAGAAAGA TGTTTATCGT CGCATCGTAG AATCCGATGT GGGTGATTCT
    TTCTATATCA GAACCCATTT TGAATATGAG AAGGAATCTC CCTATGGACT CAGTTTTAAC
    AAAGGAGAGG TGTTCCGTGT CGTGGACACT CTGTACAATG GGAAGCTGGG GTCCTGGCTT
    GCCATTCGGA TTGGCAAAAA CCATAAAGAG GTGGAACGTG GCATCATCCC AAACAAAAAC
    AGAGCTGAGC AGTTAGCCAG TGTGCAGTAT ACACTTCCCA AGACAGCAGG GGGAGACCGC
    GCCGACTTCT GGAGGTTCCG CGGTCTGCGC AGCTCCAAGA GAAACCTGCG CAAGAGCCGA
    GAAGACCTCT CAGCCCAGCC AGTCCAGACC AAGTTCCCGG CGTATGAGAG GGTGGTTCTT
    CGAGAAGCTG GATTCCTGAG GCCTGTAACC ATCTTTGGAC CAATAGCTGA TGTTGCCAGG
    GAGAAACTGG CAAGAGAGGA GCCAGATATT TACCAGATCG CAAAAAGTGA ACCTCGGGAT
    GCAGGAACCG ACCAGCGCAG CTCTGGCATT ATTCGCCTCC ACACAATAAA GCAGATAATA
    GATCAGGACA AACATGCCTT ACTGGATGTA ACGCCCAATG CAGTAGACCG TCTGAACTAT
    GCCCAGTGGT ATCCAATCGT GGTGTTCCTT AACCCGGACT CAAAGCAGGG TGTGAAAACA
    ATGCGAATGA GGTTATGTCC AGAATCTCGG AAAAGCGCCA GGAAGTTATA TGAGCGCTCT
    CATAAACTTC GTAAAAATAA TCACCATCTT TTTACAACTA CAATTAACTT AAATTCAATG
    AATGATGGCT GGTATGGTGC ACTGAAAGAA GCAATCCAAC AACAACAGAA CCAGTTGGTA
    TGGGTTTCTG AGGGAAAGGC GGATGGTGCT ACAAGTGATG ACCTTGATTT GCATGATGAT
    CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT GAATACTCAA TGTATAGCAC GGACAGTAGA
    CACACTTCTG ACTATGAAGA CACAGACACA GAAGGCGGGG CCTACACTGA TCAAGAACTA
    GATGAGACCC TAAATGATGA GGTGGGGACT CCCCCGGAGT CTGCCATTAC CCGGTCCTCT
    GAGCCTGTCA GAGAGGACTC CTCTGGAATG CATCCTGACC ACCAGGCATA TCCTCCCTAC
    TCACCACAAG CGCAGCCACA GCCAGGCCAT AGAATAGACT CCCCTGGATT TAAAACAGCC
    TCTCAACAGA AAGCAGAAGC CTCATCTCCA GGCCCTTACC TTTCGCCTGA AACAAACCCG
    GCACCATCAG CCTCTGCTGT TAATCCTCAC GTAGACTTAA CTAATGTCAG ACTGGAGGAG
    CCGAGCCCAG CTCCCCCCTC CTCTGACTCA CCACACGCCG ACTCTTTACG AGCTCCAGGC
    ACTGAGGCCG CTCACGTGCA GCTCAAAGGT CCAGAGCCGT CCCTGTCGTC GCATGTGGGC
    CCAGCAAAGG TGTACAGGAA GGACCCCTAC CCTGAGGAGA TGATGAGACA GAACCACGTC
    TTGAAGCAGC CGGCTGTGGT TCACCCTGGG CAGAGGCCAG ACAAGGAGCA AGGCCTGAGC
    TACGAGCCGC AGCCTCCGTA CACAGAGCGA CAGGCTGGCA GGGAGTTGGA GCAGCCTGCA
    TACAGATACG ATGCCTCTAG CTACCCGGAG CAGTTTGCTC GGAACTATGA CCATCGTCTG
    CGCTATGACG ACCGCCTCCT CACCTATGAG GAACAATGGC CCTACTACGA TGACAAGCAG
    CCTTACCAGC CCCGGCCTCT TGATGGTCAG CCTCCCGCGG AGCCAACAGA ACGAGGGTAT
    TTCCCCCGTT TTGAGGAGCC AGTGCCCCTG TCCTATGAAG GCCGAGCGCA CTACGACCCA
    CTGCCCAGGA CCTCCACCTT GCGGCACGAA GAGCCTCCCG CCCCTGCCCC TGGGTACGAC
    GTGCACGGAA GGTACCGGCC TGACGTGCAG CCCTACTCCA CTGCTGGCCC CAAGGTGCCC
    CCTGAGCCCA AGCAGTACTT GGACCAGTAC CCTCGGAGTT ACGAGCCAGC GCCAGCCCAG
    GGCTTCCCCT CCAGGGCAGG CCCAGGCCAC TACGAGCCTC TTCACAGCAC CACGCTCGCT
    CCACCACTTG GCCCCTCGTC CCAGCACAAG CCCGAAGTCC CGCCCACAGG CACCAAACCC
    CTGCCTCCAC CCCCAGCTCC GGCCGAGGAG GAGGAAGACC CAGCCATGAA GCCACAATCC
    GTGCTCACCA GAGTGAAGAT GTTTGAGAAT AAGAGGTCTG CTTCCATGGA GAACAGGAAG
    GATGAGAGCC ATTCCACTAG TTTCAAGCCC CCAGAAGTCA CGTCAAAACC GGCAGGTGCT
    CCTGTCCCAG GTCCCAAAGC CCCTGCTCCG AGTCAGCTCG GTGAACACGA CAGAGCAGCA
    CATAGGATCC CTGAACCACA GAGGCCTCAC ATGAAGCCAC CTGAAGACAT TGTTCGGGCA
    AATCACTATG ACCCCGAGGA GGATGAGGAG TACTACCGGA AGCAGCTCTC CTACTTCGAT
    CGGAGAAGCT TTGAGGCCAA GCCCCCCACC CACATCCCCA CTAGCCACCT CTCCGAGCCC
    ACCAGGCCGG CTCACCCCCA ATCTCAGCCA CAGTTTGCCG GTTACTCTTC CAAGGGGAAG
    GCTGCTGAGG CCGAAGCTGT GGACAGGTCA TTTGGGGAGA AGCGCTATGA CCCGCTGCAG
    GCGACGCCCC CCCCTCCGCC GCTGCCTGTT CAGTACAGCC AGGGTGCTCA ACCCAGCCCC
    AACACGGCTC TTGGCCTCCA CGCACACACC AAGGGGGCGC ATGGTGAAGG TAATGCCATG
    TCGTTGGATT TTCAGAACTC GTTAGTGTCC AAACCGGACC CACCTCCATC TCAGAATAAG
    CCAGCAGCTT ACAGACCAGC CCCTCGAGAG GATGCTGTCC AGTCTGGCTT CTACCCACAA
    AAGAGTTTCC CAGAGAAAGC CCCAGCTAAT GGGGTCGAGC AGACGCAGAA AACCGTCACG
    CCAGCATACA ATCGATTCAC ACCAAAACCA TACACAAGCT CTGCACGGCC ATTTGAACGC
    AAGTTTGAAA GCCCTAAATT CAACCACAAT CTCTTGCCGA ATGAAACCGC ACACAAACCT
    GACCTGTCCT CCAAAGCCCC CGCTTCTCCG AAAACCCTCC TGAAGTCTGC ACAGCCTCCT
    GAGTTTGACA GTGGCGTGGA GACCCTCTCT GTCCACACTG ACAACAAGCT GAAATACCAA
    GTAAACAGTG TTAGCATGGG GCCGAGAGCA GTGCCCGTGA GTCCCTCAGC CGTGGAGGAA
    GATGAAGACG AAGACGGTCA CACAGTGGTG GCTACTGCCC GCGGCGTGTT TAACAGCAAT
    GGTGGGGTGC TGAGCTCCAT TGAGACCGGT GTCAGCATCA TCATCCCCCA GGGAGCCATT
    CCCGAGGGGG TGGAACAGGA GATCTACTTC AAGGTCTGCC GCGACAACAG CATCCTGCCG
    CCTTTGGACA AAGAGAAGGG TGAGACCCTG CTGAGCCCCC TAGTGATGTG TGGGCCCCAT
    GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCCTACCAC ACTGTGCGTC CATGACTCCT
    GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGATCC TAAAACCTGG
    CAAAACAAGT GTCTTCCTGG AGATCCAAAT TATCTTGTTG GAGCAAACTG TGTTTCTGTC
    CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45684
    Clone ID Related Accession (Same CDS sequence) XM_015468497.1 , XM_010817146.1
    Accession Version XM_010817146.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5118bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104535.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAAGTACC AGAAGTACCT GACGGCGCTG CAGATGGCCA TCGGCGTCAC GCCGACCAAC 
    CGCGGCAGCC TGCTGCCGCT CAAGAGGAGG CTGTGGGTAA CGCCGTCCTC TGAAAACCCC
    AGTGGTGCTA CTTCTAGTGT CAGCCAAGGA AAGCCTTCTT TAAGACGAAT TAAAGGGAGA
    CTGCACAGGA GCAAGAGCCT CGATAGCATC GATTTCTGTG AACTCACTAA CACAACAATG
    GAGGAAACAG CTATATGGGA ACAACACACG GTGACACTTC ACAGGGCTCC TGGATTTGGA
    TTTGGAATTG CAATATCTGG TGGAAGAGAT AATCCACATT TCCAGAGCGG GGAAACCTCC
    ATCGTGATTT CAGATGTGCT GAAGGGGGGG CCTGCGGAAG GACAGCTGCA GGAAAATGAC
    CGGGTGGCAA TGGTTAATGG AGTCTCCATG GATAATGTTG AGCATGCGTT TGCTGTTCAG
    CAACTAAGGA AAAGTGGGAA AAATGCAAAA ATAACCATTC GAAGGAAGAA GAAAGTTCAG
    ATCCCAGTAA ATCGTCCTGA TCCTGAGCCT GTGTCTGAGA ACGAAGAGGA CAGTTACGAC
    GAGGAAGCGC ATGACCCGAG AAGTGGCCGT GGGGCCCTGG CAAACAGGAG GAGCGAGAAG
    AGCTGGGGCC GAGATAGGAG TGCCAGTCGG GAGAGGAGTC TGTCCCCGCG GTCTGACAGG
    CGGTCTGTAG CCTCCAGTCA GCCTGCTAGA CCCACCAAAG TCACCCTGGT GAAGTCCCGG
    AAGAATGAAG GTATTTTCTG CCAATCTAGC CTTTTCACAG TTTTACTAAT GTACAGTCTC
    CGGAGCAGAG AGGAAGAGAG AATTTCTAAA CCAGGGGCTG TCTCGACTCC GGTAAAGCAT
    GCAGATGATC ATACACCTAA AACAGTGGAA GAAGTTGTAG TTGAGCGAAA TGAGAAACAA
    GCACCCAATC TTCCAGAACC AAAGCCTGTG TACGCACAGG TAGGACAGCC AGACGTGGAT
    TTACCTGTCA GTCCATCTGA TGGCGTCCTG CCCAACTCAA CTCATGAAGA TGGCATTCTT
    CGGCCCAGCA TGAAGTTAGT AAAATTCAGG AAAGGAGATA GTGTGGGTCT TCGACTAGCT
    GGTGGAAACG ATGTTGGCAT ATTTGTAGCT GGTGTTCTCG AAGATAGCCC TGCTGCCAAA
    GAAGGCTTGG AGGAGGGCGA CCAGATCCTC AGGGTAAACA ATGTAGATTT CACAAATATC
    ATAAGAGAAG AAGCCGTCCT CTTCCTGCTT GACCTCCCAA AAGGAGAAGA AGTGACCATA
    TTGGCTCAGA AGAAGAAAGA TGTTTATCGT CGCATCGTAG AATCCGATGT GGGTGATTCT
    TTCTATATCA GAACCCATTT TGAATATGAG AAGGAATCTC CCTATGGACT CAGTTTTAAC
    AAAGGAGAGG TGTTCCGTGT CGTGGACACT CTGTACAATG GGAAGCTGGG GTCCTGGCTT
    GCCATTCGGA TTGGCAAAAA CCATAAAGAG GTGGAACGTG GCATCATCCC AAACAAAAAC
    AGAGCTGAGC AGTTAGCCAG TGTGCAGTAT ACACTTCCCA AGACAGCAGG GGGAGACCGC
    GCCGACTTCT GGAGGTTCCG CGGTCTGCGC AGCTCCAAGA GAAACCTGCG CAAGAGCCGA
    GAAGACCTCT CAGCCCAGCC AGTCCAGACC AAGTTCCCGG CGTATGAGAG GGTGGTTCTT
    CGAGAAGCTG GATTCCTGAG GCCTGTAACC ATCTTTGGAC CAATAGCTGA TGTTGCCAGG
    GAGAAACTGG CAAGAGAGGA GCCAGATATT TACCAGATCG CAAAAAGTGA ACCTCGGGAT
    GCAGGAACCG ACCAGCGCAG CTCTGGCATT ATTCGCCTCC ACACAATAAA GCAGATAATA
    GATCAGGACA AACATGCCTT ACTGGATGTA ACGCCCAATG CAGTAGACCG TCTGAACTAT
    GCCCAGTGGT ATCCAATCGT GGTGTTCCTT AACCCGGACT CAAAGCAGGG TGTGAAAACA
    ATGCGAATGA GGTTATGTCC AGAATCTCGG AAAAGCGCCA GGAAGTTATA TGAGCGCTCT
    CATAAACTTC GTAAAAATAA TCACCATCTT TTTACAACTA CAATTAACTT AAATTCAATG
    AATGATGGCT GGTATGGTGC ACTGAAAGAA GCAATCCAAC AACAACAGAA CCAGTTGGTA
    TGGGTTTCTG AGGGAAAGGC GGATGGTGCT ACAAGTGATG ACCTTGATTT GCATGATGAT
    CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT GAATACTCAA TGTATAGCAC GGACAGTAGA
    CACACTTCTG ACTATGAAGA CACAGACACA GAAGGCGGGG CCTACACTGA TCAAGAACTA
    GATGAGACCC TAAATGATGA GGTGGGGACT CCCCCGGAGT CTGCCATTAC CCGGTCCTCT
    GAGCCTGTCA GAGAGGACTC CTCTGGAATG CATCCTGACC ACCAGGCATA TCCTCCCTAC
    TCACCACAAG CGCAGCCACA GCCAGGCCAT AGAATAGACT CCCCTGGATT TAAAACAGCC
    TCTCAACAGA AAGCAGAAGC CTCATCTCCA GGCCCTTACC TTTCGCCTGA AACAAACCCG
    GCACCATCAG CCTCTGCTGT TAATCCTCAC GTAGACTTAA CTAATGTCAG ACTGGAGGAG
    CCGAGCCCAG CTCCCCCCTC CTCTGACTCA CCACACGCCG ACTCTTTACG AGCTCCAGGC
    ACTGAGGCCG CTCACGTGCA GCTCAAAGGT CCAGAGCCGT CCCTGTCGTC GCATGTGGGC
    CCAGCAAAGG TGTACAGGAA GGACCCCTAC CCTGAGGAGA TGATGAGACA GAACCACGTC
    TTGAAGCAGC CGGCTGTGGT TCACCCTGGG CAGAGGCCAG ACAAGGAGCA AGGCCTGAGC
    TACGAGCCGC AGCCTCCGTA CACAGAGCGA CAGGCTGGCA GGGAGTTGGA GCAGCCTGCA
    TACAGATACG ATGCCTCTAG CTACCCGGAG CAGTTTGCTC GGAACTATGA CCATCGTCTG
    CGCTATGACG ACCGCCTCCT CACCTATGAG GAACAATGGC CCTACTACGA TGACAAGCAG
    CCTTACCAGC CCCGGCCTCT TGATGGTCAG CCTCCCGCGG AGCCAACAGA ACGAGGGTAT
    TTCCCCCGTT TTGAGGAGCC AGTGCCCCTG TCCTATGAAG GCCGAGCGCA CTACGACCCA
    CTGCCCAGGA CCTCCACCTT GCGGCACGAA GAGCCTCCCG CCCCTGCCCC TGGGTACGAC
    GTGCACGGAA GGTACCGGCC TGACGTGCAG CCCTACTCCA CTGCTGGCCC CAAGGTGCCC
    CCTGAGCCCA AGCAGTACTT GGACCAGTAC CCTCGGAGTT ACGAGCCAGC GCCAGCCCAG
    GGCTTCCCCT CCAGGGCAGG CCCAGGCCAC TACGAGCCTC TTCACAGCAC CACGCTCGCT
    CCACCACTTG GCCCCTCGTC CCAGCACAAG CCCGAAGTCC CGCCCACAGG CACCAAACCC
    CTGCCTCCAC CCCCAGCTCC GGCCGAGGAG GAGGAAGACC CAGCCATGAA GCCACAATCC
    GTGCTCACCA GAGTGAAGAT GTTTGAGAAT AAGAGGTCTG CTTCCATGGA GAACAGGAAG
    GATGAGAGCC ATTCCACTAG TTTCAAGCCC CCAGAAGTCA CGTCAAAACC GGCAGGTGCT
    CCTGTCCCAG GTCCCAAAGC CCCTGCTCCG AGTCAGCTCG GTGAACACGA CAGAGCAGCA
    CATAGGATCC CTGAACCACA GAGGCCTCAC ATGAAGCCAC CTGAAGACAT TGTTCGGGCA
    AATCACTATG ACCCCGAGGA GGATGAGGAG TACTACCGGA AGCAGCTCTC CTACTTCGAT
    CGGAGAAGCT TTGAGGCCAA GCCCCCCACC CACATCCCCA CTAGCCACCT CTCCGAGCCC
    ACCAGGCCGG CTCACCCCCA ATCTCAGCCA CAGTTTGCCG GTTACTCTTC CAAGGGGAAG
    GCTGCTGAGG CCGAAGCTGT GGACAGGTCA TTTGGGGAGA AGCGCTATGA CCCGCTGCAG
    GCGACGCCCC CCCCTCCGCC GCTGCCTGTT CAGTACAGCC AGGGTGCTCA ACCCAGCCCC
    AACACGGCTC TTGGCCTCCA CGCACACACC AAGGGGGCGC ATGGTGAAGG TAATGCCATG
    TCGTTGGATT TTCAGAACTC GTTAGTGTCC AAACCGGACC CACCTCCATC TCAGAATAAG
    CCAGCAGCTT ACAGACCAGC CCCTCGAGAG GATGCTGTCC AGTCTGGCTT CTACCCACAA
    AAGAGTTTCC CAGAGAAAGC CCCAGCTAAT GGGGTCGAGC AGACGCAGAA AACCGTCACG
    CCAGCATACA ATCGATTCAC ACCAAAACCA TACACAAGCT CTGCACGGCC ATTTGAACGC
    AAGTTTGAAA GCCCTAAATT CAACCACAAT CTCTTGCCGA ATGAAACCGC ACACAAACCT
    GACCTGTCCT CCAAAGCCCC CGCTTCTCCG AAAACCCTCC TGAAGTCTGC ACAGCCTCCT
    GAGTTTGACA GTGGCGTGGA GACCCTCTCT GTCCACACTG ACAACAAGCT GAAATACCAA
    GTAAACAGTG TTAGCATGGG GCCGAGAGCA GTGCCCGTGA GTCCCTCAGC CGTGGAGGAA
    GATGAAGACG AAGACGGTCA CACAGTGGTG GCTACTGCCC GCGGCGTGTT TAACAGCAAT
    GGTGGGGTGC TGAGCTCCAT TGAGACCGGT GTCAGCATCA TCATCCCCCA GGGAGCCATT
    CCCGAGGGGG TGGAACAGGA GATCTACTTC AAGGTCTGCC GCGACAACAG CATCCTGCCG
    CCTTTGGACA AAGAGAAGGG TGAGACCCTG CTGAGCCCCC TAGTGATGTG TGGGCCCCAT
    GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCCTACCAC ACTGTGCGTC CATGACTCCT
    GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGATCC TAAAACCTGG
    CAAAACAAGT GTCTTCCTGG AGATCCAAAT TATCTTGTTG GAGCAAACTG TGTTTCTGTC
    CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010815448.1
    CDS16..5133
    Translation

    Target ORF information:

    RefSeq Version XM_010817146.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 1 (TJP1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817146.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAAGTACC AGAAGTACCT GACGGCGCTG CAGATGGCCA TCGGCGTCAC GCCGACCAAC 
    CGCGGCAGCC TGCTGCCGCT CAAGAGGAGG CTGTGGGTAA CGCCGTCCTC TGAAAACCCC
    AGTGGTGCTA CTTCTAGTGT CAGCCAAGGA AAGCCTTCTT TAAGACGAAT TAAAGGGAGA
    CTGCACAGGA GCAAGAGCCT CGATAGCATC GATTTCTGTG AACTCACTAA CACAACAATG
    GAGGAAACAG CTATATGGGA ACAACACACG GTGACACTTC ACAGGGCTCC TGGATTTGGA
    TTTGGAATTG CAATATCTGG TGGAAGAGAT AATCCACATT TCCAGAGCGG GGAAACCTCC
    ATCGTGATTT CAGATGTGCT GAAGGGGGGG CCTGCGGAAG GACAGCTGCA GGAAAATGAC
    CGGGTGGCAA TGGTTAATGG AGTCTCCATG GATAATGTTG AGCATGCGTT TGCTGTTCAG
    CAACTAAGGA AAAGTGGGAA AAATGCAAAA ATAACCATTC GAAGGAAGAA GAAAGTTCAG
    ATCCCAGTAA ATCGTCCTGA TCCTGAGCCT GTGTCTGAGA ACGAAGAGGA CAGTTACGAC
    GAGGAAGCGC ATGACCCGAG AAGTGGCCGT GGGGCCCTGG CAAACAGGAG GAGCGAGAAG
    AGCTGGGGCC GAGATAGGAG TGCCAGTCGG GAGAGGAGTC TGTCCCCGCG GTCTGACAGG
    CGGTCTGTAG CCTCCAGTCA GCCTGCTAGA CCCACCAAAG TCACCCTGGT GAAGTCCCGG
    AAGAATGAAG GTATTTTCTG CCAATCTAGC CTTTTCACAG TTTTACTAAT GTACAGTCTC
    CGGAGCAGAG AGGAAGAGAG AATTTCTAAA CCAGGGGCTG TCTCGACTCC GGTAAAGCAT
    GCAGATGATC ATACACCTAA AACAGTGGAA GAAGTTGTAG TTGAGCGAAA TGAGAAACAA
    GCACCCAATC TTCCAGAACC AAAGCCTGTG TACGCACAGG TAGGACAGCC AGACGTGGAT
    TTACCTGTCA GTCCATCTGA TGGCGTCCTG CCCAACTCAA CTCATGAAGA TGGCATTCTT
    CGGCCCAGCA TGAAGTTAGT AAAATTCAGG AAAGGAGATA GTGTGGGTCT TCGACTAGCT
    GGTGGAAACG ATGTTGGCAT ATTTGTAGCT GGTGTTCTCG AAGATAGCCC TGCTGCCAAA
    GAAGGCTTGG AGGAGGGCGA CCAGATCCTC AGGGTAAACA ATGTAGATTT CACAAATATC
    ATAAGAGAAG AAGCCGTCCT CTTCCTGCTT GACCTCCCAA AAGGAGAAGA AGTGACCATA
    TTGGCTCAGA AGAAGAAAGA TGTTTATCGT CGCATCGTAG AATCCGATGT GGGTGATTCT
    TTCTATATCA GAACCCATTT TGAATATGAG AAGGAATCTC CCTATGGACT CAGTTTTAAC
    AAAGGAGAGG TGTTCCGTGT CGTGGACACT CTGTACAATG GGAAGCTGGG GTCCTGGCTT
    GCCATTCGGA TTGGCAAAAA CCATAAAGAG GTGGAACGTG GCATCATCCC AAACAAAAAC
    AGAGCTGAGC AGTTAGCCAG TGTGCAGTAT ACACTTCCCA AGACAGCAGG GGGAGACCGC
    GCCGACTTCT GGAGGTTCCG CGGTCTGCGC AGCTCCAAGA GAAACCTGCG CAAGAGCCGA
    GAAGACCTCT CAGCCCAGCC AGTCCAGACC AAGTTCCCGG CGTATGAGAG GGTGGTTCTT
    CGAGAAGCTG GATTCCTGAG GCCTGTAACC ATCTTTGGAC CAATAGCTGA TGTTGCCAGG
    GAGAAACTGG CAAGAGAGGA GCCAGATATT TACCAGATCG CAAAAAGTGA ACCTCGGGAT
    GCAGGAACCG ACCAGCGCAG CTCTGGCATT ATTCGCCTCC ACACAATAAA GCAGATAATA
    GATCAGGACA AACATGCCTT ACTGGATGTA ACGCCCAATG CAGTAGACCG TCTGAACTAT
    GCCCAGTGGT ATCCAATCGT GGTGTTCCTT AACCCGGACT CAAAGCAGGG TGTGAAAACA
    ATGCGAATGA GGTTATGTCC AGAATCTCGG AAAAGCGCCA GGAAGTTATA TGAGCGCTCT
    CATAAACTTC GTAAAAATAA TCACCATCTT TTTACAACTA CAATTAACTT AAATTCAATG
    AATGATGGCT GGTATGGTGC ACTGAAAGAA GCAATCCAAC AACAACAGAA CCAGTTGGTA
    TGGGTTTCTG AGGGAAAGGC GGATGGTGCT ACAAGTGATG ACCTTGATTT GCATGATGAT
    CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT GAATACTCAA TGTATAGCAC GGACAGTAGA
    CACACTTCTG ACTATGAAGA CACAGACACA GAAGGCGGGG CCTACACTGA TCAAGAACTA
    GATGAGACCC TAAATGATGA GGTGGGGACT CCCCCGGAGT CTGCCATTAC CCGGTCCTCT
    GAGCCTGTCA GAGAGGACTC CTCTGGAATG CATCCTGACC ACCAGGCATA TCCTCCCTAC
    TCACCACAAG CGCAGCCACA GCCAGGCCAT AGAATAGACT CCCCTGGATT TAAAACAGCC
    TCTCAACAGA AAGCAGAAGC CTCATCTCCA GGCCCTTACC TTTCGCCTGA AACAAACCCG
    GCACCATCAG CCTCTGCTGT TAATCCTCAC GTAGACTTAA CTAATGTCAG ACTGGAGGAG
    CCGAGCCCAG CTCCCCCCTC CTCTGACTCA CCACACGCCG ACTCTTTACG AGCTCCAGGC
    ACTGAGGCCG CTCACGTGCA GCTCAAAGGT CCAGAGCCGT CCCTGTCGTC GCATGTGGGC
    CCAGCAAAGG TGTACAGGAA GGACCCCTAC CCTGAGGAGA TGATGAGACA GAACCACGTC
    TTGAAGCAGC CGGCTGTGGT TCACCCTGGG CAGAGGCCAG ACAAGGAGCA AGGCCTGAGC
    TACGAGCCGC AGCCTCCGTA CACAGAGCGA CAGGCTGGCA GGGAGTTGGA GCAGCCTGCA
    TACAGATACG ATGCCTCTAG CTACCCGGAG CAGTTTGCTC GGAACTATGA CCATCGTCTG
    CGCTATGACG ACCGCCTCCT CACCTATGAG GAACAATGGC CCTACTACGA TGACAAGCAG
    CCTTACCAGC CCCGGCCTCT TGATGGTCAG CCTCCCGCGG AGCCAACAGA ACGAGGGTAT
    TTCCCCCGTT TTGAGGAGCC AGTGCCCCTG TCCTATGAAG GCCGAGCGCA CTACGACCCA
    CTGCCCAGGA CCTCCACCTT GCGGCACGAA GAGCCTCCCG CCCCTGCCCC TGGGTACGAC
    GTGCACGGAA GGTACCGGCC TGACGTGCAG CCCTACTCCA CTGCTGGCCC CAAGGTGCCC
    CCTGAGCCCA AGCAGTACTT GGACCAGTAC CCTCGGAGTT ACGAGCCAGC GCCAGCCCAG
    GGCTTCCCCT CCAGGGCAGG CCCAGGCCAC TACGAGCCTC TTCACAGCAC CACGCTCGCT
    CCACCACTTG GCCCCTCGTC CCAGCACAAG CCCGAAGTCC CGCCCACAGG CACCAAACCC
    CTGCCTCCAC CCCCAGCTCC GGCCGAGGAG GAGGAAGACC CAGCCATGAA GCCACAATCC
    GTGCTCACCA GAGTGAAGAT GTTTGAGAAT AAGAGGTCTG CTTCCATGGA GAACAGGAAG
    GATGAGAGCC ATTCCACTAG TTTCAAGCCC CCAGAAGTCA CGTCAAAACC GGCAGGTGCT
    CCTGTCCCAG GTCCCAAAGC CCCTGCTCCG AGTCAGCTCG GTGAACACGA CAGAGCAGCA
    CATAGGATCC CTGAACCACA GAGGCCTCAC ATGAAGCCAC CTGAAGACAT TGTTCGGGCA
    AATCACTATG ACCCCGAGGA GGATGAGGAG TACTACCGGA AGCAGCTCTC CTACTTCGAT
    CGGAGAAGCT TTGAGGCCAA GCCCCCCACC CACATCCCCA CTAGCCACCT CTCCGAGCCC
    ACCAGGCCGG CTCACCCCCA ATCTCAGCCA CAGTTTGCCG GTTACTCTTC CAAGGGGAAG
    GCTGCTGAGG CCGAAGCTGT GGACAGGTCA TTTGGGGAGA AGCGCTATGA CCCGCTGCAG
    GCGACGCCCC CCCCTCCGCC GCTGCCTGTT CAGTACAGCC AGGGTGCTCA ACCCAGCCCC
    AACACGGCTC TTGGCCTCCA CGCACACACC AAGGGGGCGC ATGGTGAAGG TAATGCCATG
    TCGTTGGATT TTCAGAACTC GTTAGTGTCC AAACCGGACC CACCTCCATC TCAGAATAAG
    CCAGCAGCTT ACAGACCAGC CCCTCGAGAG GATGCTGTCC AGTCTGGCTT CTACCCACAA
    AAGAGTTTCC CAGAGAAAGC CCCAGCTAAT GGGGTCGAGC AGACGCAGAA AACCGTCACG
    CCAGCATACA ATCGATTCAC ACCAAAACCA TACACAAGCT CTGCACGGCC ATTTGAACGC
    AAGTTTGAAA GCCCTAAATT CAACCACAAT CTCTTGCCGA ATGAAACCGC ACACAAACCT
    GACCTGTCCT CCAAAGCCCC CGCTTCTCCG AAAACCCTCC TGAAGTCTGC ACAGCCTCCT
    GAGTTTGACA GTGGCGTGGA GACCCTCTCT GTCCACACTG ACAACAAGCT GAAATACCAA
    GTAAACAGTG TTAGCATGGG GCCGAGAGCA GTGCCCGTGA GTCCCTCAGC CGTGGAGGAA
    GATGAAGACG AAGACGGTCA CACAGTGGTG GCTACTGCCC GCGGCGTGTT TAACAGCAAT
    GGTGGGGTGC TGAGCTCCAT TGAGACCGGT GTCAGCATCA TCATCCCCCA GGGAGCCATT
    CCCGAGGGGG TGGAACAGGA GATCTACTTC AAGGTCTGCC GCGACAACAG CATCCTGCCG
    CCTTTGGACA AAGAGAAGGG TGAGACCCTG CTGAGCCCCC TAGTGATGTG TGGGCCCCAT
    GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCCTACCAC ACTGTGCGTC CATGACTCCT
    GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGATCC TAAAACCTGG
    CAAAACAAGT GTCTTCCTGG AGATCCAAAT TATCTTGTTG GAGCAAACTG TGTTTCTGTC
    CTGATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.