ASMT cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ASMT
Entrez Gene ID	281013
Full Name	acetylserotonin O-methyltransferase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_177493.2	NP_803459.1	acetylserotonin O-methyltransferase
XM_024989756.1	XP_024845524.1	acetylserotonin O-methyltransferase isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Evidence of melatonin synthesis in the cumulus oocyte complexes and its role in enhancing oocyte maturation in vitro in cattle.
El-Raey M, Geshi M, Somfai T, Kaneda M, Hirako M, Abdel-Ghaffar AE, Sosa GA, El-Roos ME, Nagai T
Molecular reproduction and development78(4)250-62(2011 Apr)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Bovine hydroxyindole-O-methyltransferase. Significant sequence revision.
Donohue SJ, Roseboom PH, Klein DC
The Journal of biological chemistry267(8)5184-5(1992 Mar)

Molecular cloning and nucleotide sequence of cDNA encoding hydroxyindole O-methyltransferase of bovine pineal glands.
Ishida I, Obinata M, Deguchi T
The Journal of biological chemistry262(6)2895-9(1987 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The study revealed that ASMT and MTNR1A genes were expressed in cumulus-oocyte complexes (COCs).
Title: Evidence of melatonin synthesis in the cumulus oocyte complexes and its role in enhancing oocyte maturation in vitro in cattle.

The following ASMT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASMT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa41825	NM_177493.2 Latest version!	Bos taurus acetylserotonin O-methyltransferase (ASMT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa218594	XM_024989756.1 Latest version!	Bos taurus acetylserotonin O-methyltransferase (ASMT), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ASMT ( NM_177493.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_803459.1 Bos taurus acetylserotonin O-methyltransferase (ASMT), mRNA.

Price & Availability↑

CloneID	OBa41825
Clone ID Related Accession (Same CDS sequence)	NM_177493.2
Accession Version	NM_177493.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	acetylserotonin O-methyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J02671.1. On Jun 3, 2003 this sequence version replaced NM_177493.1. ##Evidence-Data-START## Transcript exon combination :: J02671.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145467 [ECO:0000348] ##Evidence-Data-END##

ATGTGCTCCC AGGAGGGTGA GGGCTACAGT CTCCTGAAGG AATACGCCAA CGCGTTCATG 
GTCTCCCAGG TTCTCTTTGC TGCCTGTGAG CTGGGGGTGT TTGAGCTCCT GGCAGAGGCC 
CTGGAGCCCC TGGACTCAGC AGCAGTGTCT TCACATCTGG GCTCCAGCCC AGGGGACAGA 
GCTGCTACTG AACACCTGTG TGTCCCTGAA GCTGCTGCAA GCCGACGTGA GGGGAGGAAA 
AGCTGTGTAT GCAAACACGG AGCTCGCCAG CACCTACCTG GTGAGAGGCA GCCCCAGGTC 
CCAGCGGGAC ATGCTGCTGT ACGCGGGCAG GACCGCTTAC GTCTGCTGGC GCCACCTGGC 
GAGGCAGTCA GGGAAGGGAG GAACCAGTAT CTGAAAGCCT TCGGGATCCC GTCCGAGGAG 
CTCTTCTCTG CCATCTACAG GTCTGAGGAC GAAAGGCTGC AGTTCATGCA GGGTCTGCAG 
GACGTGTGGA GGCTGGAGGG GGCGACGGTG CTGGCCGCCT TCGACCTGTC CCCCTTCCCG 
CTCATCTGCG ACCTGGGGGG CGGTTCTGGG GCTCTGGCCA AGGCGTGCGT GTCCCTGTAC 
CCGGGATGTC GGGCCATTGT GTTCGACATC CCAGGAGTGG TGCAGATCGC CAAGAGACAT 
TTCTCAGCCT CGGAGGACGA GCGCATCAGC TTCCATGAAG GGGATTTCTT CAAAGACGCC 
CTCCCGGAGG CGGACCTGTA TATCCTGGCC AGGGTCCTGC ATGACTGGAC CGATGCCAAG 
TGCTCCCACC TGCTGCAGAG AGTCTATCGG GCCTGCAGGA CAGGTGGCGG CATCCTGGTC 
ATCGAGAGCC TCCTGGACAC GGACGGGCGG GGCCCTCTGA CCACGCTGCT GTACTCGCTG 
AACATGCTGG TGCAAACGGA GGGCCGTGAG CGGACCCCCG GCCGAAGTAC CGCGCGCTCC 
GTGGGCCCCG CCGCTTCCGA GACGTGCGGT GACGGAGGAC GGGGGGAACC TACGATGCTG 
TCTTGGCCAG GAAATCAGGC CTGCAGCGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_803459.1
CDS	124..1176
Translation	MCSQEGEGYSLLKEYANAFMVSQVLFAACELGVFELLAEALEPLDSAAVSSHLGSSPGDRAATEHLCVPEAAASRREGRKSCVCKHGARQHLPGERQPQVPAGHAAVRGQDRLRLLAPPGEAVREGRNQYLKAFGIPSEELFSAIYRSEDERLQFMQGLQDVWRLEGATVLAAFDLSPFPLICDLGGGSGALAKACVSLYPGCRAIVFDIPGVVQIAKRHFSASEDERISFHEGDFFKDALPEADLYILARVLHDWTDAKCSHLLQRVYRACRTGGGILVIESLLDTDGRGPLTTLLYSLNMLVQTEGRERTPGRSTARSVGPAASETCGDGGRGEPTMLSWPGNQACSV

Target ORF information:

RefSeq Version	NM_177493.2
Organism	Bos taurus(cattle)
Definition	Bos taurus acetylserotonin O-methyltransferase (ASMT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177493.2

ORF Insert Sequence:

ATGTGCTCCC AGGAGGGTGA GGGCTACAGT CTCCTGAAGG AATACGCCAA CGCGTTCATG 
GTCTCCCAGG TTCTCTTTGC TGCCTGTGAG CTGGGGGTGT TTGAGCTCCT GGCAGAGGCC 
CTGGAGCCCC TGGACTCAGC AGCAGTGTCT TCACATCTGG GCTCCAGCCC AGGGGACAGA 
GCTGCTACTG AACACCTGTG TGTCCCTGAA GCTGCTGCAA GCCGACGTGA GGGGAGGAAA 
AGCTGTGTAT GCAAACACGG AGCTCGCCAG CACCTACCTG GTGAGAGGCA GCCCCAGGTC 
CCAGCGGGAC ATGCTGCTGT ACGCGGGCAG GACCGCTTAC GTCTGCTGGC GCCACCTGGC 
GAGGCAGTCA GGGAAGGGAG GAACCAGTAT CTGAAAGCCT TCGGGATCCC GTCCGAGGAG 
CTCTTCTCTG CCATCTACAG GTCTGAGGAC GAAAGGCTGC AGTTCATGCA GGGTCTGCAG 
GACGTGTGGA GGCTGGAGGG GGCGACGGTG CTGGCCGCCT TCGACCTGTC CCCCTTCCCG 
CTCATCTGCG ACCTGGGGGG CGGTTCTGGG GCTCTGGCCA AGGCGTGCGT GTCCCTGTAC 
CCGGGATGTC GGGCCATTGT GTTCGACATC CCAGGAGTGG TGCAGATCGC CAAGAGACAT 
TTCTCAGCCT CGGAGGACGA GCGCATCAGC TTCCATGAAG GGGATTTCTT CAAAGACGCC 
CTCCCGGAGG CGGACCTGTA TATCCTGGCC AGGGTCCTGC ATGACTGGAC CGATGCCAAG 
TGCTCCCACC TGCTGCAGAG AGTCTATCGG GCCTGCAGGA CAGGTGGCGG CATCCTGGTC 
ATCGAGAGCC TCCTGGACAC GGACGGGCGG GGCCCTCTGA CCACGCTGCT GTACTCGCTG 
AACATGCTGG TGCAAACGGA GGGCCGTGAG CGGACCCCCG GCCGAAGTAC CGCGCGCTCC 
GTGGGCCCCG CCGCTTCCGA GACGTGCGGT GACGGAGGAC GGGGGGAACC TACGATGCTG 
TCTTGGCCAG GAAATCAGGC CTGCAGCGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASMT ( XM_024989756.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024845524.1 Bos taurus acetylserotonin O-methyltransferase (ASMT), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa218594
Clone ID Related Accession (Same CDS sequence)	XM_024989756.1
Accession Version	XM_024989756.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	acetylserotonin O-methyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTCCC AGGAGGGTGA GGGCTACAGT CTCCTGAAGG AATACGCCAA CGGCTTCATG 
GTCTCCCAGG TTCTCTTTGC TGCCTGTGAG CTGGGGGTGT TTGAGCTCCT GGCAGAGGCC 
CTGGAGCCCC TGGACTCAGC AGCAGTGTCT TCACATCTGG GCTCCAGCCC CCAGGGGACA 
GAGCTGCTAC TGAACACCTG TGTGTCCCTG AAGCTGCTGC AAGCCGATGT GAGGGGAGGA 
AAAGCTGTGT ATGCAAACAC GGAGCTCGCC AGCACCTACC TGGTGAGAGG CAGCCCCAGG 
TCCCAGCGGG ACATGCTGCT GTACGCGGGC AGGACCGCTT ACGTCTGCTG GCGCCACCTG 
GCGGAGGCAG TCAGGGAAGG GAGGAACCAG TATCTGAAAG CCTTCGGGAT CCCGTCCGAG 
GAGCTCTTCT CTGCCATCTA CAGGTCCGAG GACGAAAGGC TGCAGTTCAT GCAGGGTCTG 
CAGGACGTGT GGAGGCTGGA GGGGGCGACG GTGCTGGCCG CCTTCGACCT GTCCCCCTTC 
CCGCTCATCT GCGACCTGGG GGGCGGTTCT GGGGCTCTGG CCAAGGCGTG CGTGTCCCTG 
TACCCGGGAT GTCGGGCCAT TGTGTTCGAC ATCCCAGGAG TGGTGCAGAT CGCCAAGAGA 
CATTTCTCAG CCTCGGAGGA CGAGCGCATC AGCTTCCATG AAGGGGATTT CTTCAAAGAC 
GCCCTCCCGG AGGCGGACCT GTATATCCTG GCCAGGGTCC TGCATGACTG GACCGATGCC 
AAGTGCTCCC ACCTGCTGCA GAGAGTCTAT CGGGCCTGCA GGACAGGTGG CGGCATCCTG 
GTCATCGAGA GCCTCCTGGA CACGGACGGG CGGGGCCCTC TGACCACGCT GCTGTACTCG 
CTGAACATGC TGGTGCAAAC GGAGGGCCGT GAGCGGACCC CCGCCGAGTA CCGCGCGCTC 
CTGGGCCCCG CCGGCTTCCG AGACGTGCGG TGCCGGAGGA CGGGGGGAAC CTACGATGCT 
GTCTTGGCCA GGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024845524.1
CDS	124..1161
Translation	MCSQEGEGYSLLKEYANGFMVSQVLFAACELGVFELLAEALEPLDSAAVSSHLGSSPQGTELLLNTCVSLKLLQADVRGGKAVYANTELASTYLVRGSPRSQRDMLLYAGRTAYVCWRHLAEAVREGRNQYLKAFGIPSEELFSAIYRSEDERLQFMQGLQDVWRLEGATVLAAFDLSPFPLICDLGGGSGALAKACVSLYPGCRAIVFDIPGVVQIAKRHFSASEDERISFHEGDFFKDALPEADLYILARVLHDWTDAKCSHLLQRVYRACRTGGGILVIESLLDTDGRGPLTTLLYSLNMLVQTEGRERTPAEYRALLGPAGFRDVRCRRTGGTYDAVLARK

Target ORF information:

RefSeq Version	XM_024989756.1
Organism	Bos taurus(cattle)
Definition	Bos taurus acetylserotonin O-methyltransferase (ASMT), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024989756.1

ORF Insert Sequence:

ATGTGCTCCC AGGAGGGTGA GGGCTACAGT CTCCTGAAGG AATACGCCAA CGGCTTCATG 
GTCTCCCAGG TTCTCTTTGC TGCCTGTGAG CTGGGGGTGT TTGAGCTCCT GGCAGAGGCC 
CTGGAGCCCC TGGACTCAGC AGCAGTGTCT TCACATCTGG GCTCCAGCCC CCAGGGGACA 
GAGCTGCTAC TGAACACCTG TGTGTCCCTG AAGCTGCTGC AAGCCGATGT GAGGGGAGGA 
AAAGCTGTGT ATGCAAACAC GGAGCTCGCC AGCACCTACC TGGTGAGAGG CAGCCCCAGG 
TCCCAGCGGG ACATGCTGCT GTACGCGGGC AGGACCGCTT ACGTCTGCTG GCGCCACCTG 
GCGGAGGCAG TCAGGGAAGG GAGGAACCAG TATCTGAAAG CCTTCGGGAT CCCGTCCGAG 
GAGCTCTTCT CTGCCATCTA CAGGTCCGAG GACGAAAGGC TGCAGTTCAT GCAGGGTCTG 
CAGGACGTGT GGAGGCTGGA GGGGGCGACG GTGCTGGCCG CCTTCGACCT GTCCCCCTTC 
CCGCTCATCT GCGACCTGGG GGGCGGTTCT GGGGCTCTGG CCAAGGCGTG CGTGTCCCTG 
TACCCGGGAT GTCGGGCCAT TGTGTTCGAC ATCCCAGGAG TGGTGCAGAT CGCCAAGAGA 
CATTTCTCAG CCTCGGAGGA CGAGCGCATC AGCTTCCATG AAGGGGATTT CTTCAAAGAC 
GCCCTCCCGG AGGCGGACCT GTATATCCTG GCCAGGGTCC TGCATGACTG GACCGATGCC 
AAGTGCTCCC ACCTGCTGCA GAGAGTCTAT CGGGCCTGCA GGACAGGTGG CGGCATCCTG 
GTCATCGAGA GCCTCCTGGA CACGGACGGG CGGGGCCCTC TGACCACGCT GCTGTACTCG 
CTGAACATGC TGGTGCAAAC GGAGGGCCGT GAGCGGACCC CCGCCGAGTA CCGCGCGCTC 
CTGGGCCCCG CCGGCTTCCG AGACGTGCGG TGCCGGAGGA CGGGGGGAAC CTACGATGCT 
GTCTTGGCCA GGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evidence of melatonin synthesis in the cumulus oocyte complexes and its role in enhancing oocyte maturation in vitro in cattle.
Molecular reproduction and development78(4)250-62(2011 Apr)
El-Raey M,Geshi M,Somfai T,Kaneda M,Hirako M,Abdel-Ghaffar AE,Sosa GA,El-Roos ME,Nagai T

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Bovine hydroxyindole-O-methyltransferase. Significant sequence revision.
The Journal of biological chemistry267(8)5184-5(1992 Mar)
Donohue SJ,Roseboom PH,Klein DC

Molecular cloning and nucleotide sequence of cDNA encoding hydroxyindole O-methyltransferase of bovine pineal glands.
The Journal of biological chemistry262(6)2895-9(1987 Feb)
Ishida I,Obinata M,Deguchi T

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ASMT cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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