ACAN cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACAN
Entrez Gene ID	280985
Full Name	aggrecan
Synonyms	AGC1
General protein information	Preferred Names aggrecan Names aggrecan core protein CSPCP aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122) aggrecan epidermal growth factor-like domain-1 cartilage-specific proteoglycan core protein
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024981588.1	XP_024837356.1	aggrecan core protein isoform X1
XM_024981589.1	XP_024837357.1	aggrecan core protein isoform X2
XM_005221803.3	XP_005221860.1	aggrecan core protein isoform X1
XM_024981590.1	XP_024837358.1	aggrecan core protein isoform X3
NM_173981.2	NP_776406.1	aggrecan core protein precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ACAN	NP_776406.1
Mus musculus (house mouse)	Acan	NP_031450.2
Pan troglodytes (chimpanzee)	ACAN	XP_003952775.1
Rattus norvegicus (Norway rat)	Acan	NP_071526.1
	ACAN	XP_002804990.1

Gene Ontology
Bibliography

Related articles in PubMed

Antigen-specific B lymphocytes acquire proteoglycan aggrecan from cartilage extracellular matrix resulting in antigen presentation and CD4+ T-cell activation.
Ciechomska M, Wilson CL, Floudas A, Hui W, Rowan AD, van Eden W, Robinson JH, Knight AM
Immunology141(1)70-8(2014 Jan)

Microvesicles shed by oligodendroglioma cells and rheumatoid synovial fibroblasts contain aggrecanase activity.
Lo Cicero A, Majkowska I, Nagase H, Di Liegro I, Troeberg L
Matrix biology : journal of the International Society for Matrix Biology31(4)229-33(2012 May)

Disc strain and resulting positive mRNA expression from application of a noninvasive treatment.
Desmoulin GT, Hewitt CR, Hunter CJ
Spine36(14)E921-8(2011 Jun)

MMPs are less efficient than ADAMTS5 in cleaving aggrecan core protein.
Durigova M, Nagase H, Mort JS, Roughley PJ
Matrix biology : journal of the International Society for Matrix Biology30(2)145-53(2011 Mar)

Single-molecule force spectroscopy of cartilage aggrecan self-adhesion.
Harder A, Walhorn V, Dierks T, Fern?ndez-Busquets X, Anselmetti D
Biophysical journal99(10)3498-504(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the subsequent presentation of aggregan from ECM leads to CD4(+) T-cell activation and effector cell formation.
Title: Antigen-specific B lymphocytes acquire proteoglycan aggrecan from cartilage extracellular matrix resulting in antigen presentation and CD4+ T-cell activation.

Data show that expression of mRNAs for aggrecan, collagen type II, and versican were significantly effected by the intervention.
Title: Disc strain and resulting positive mRNA expression from application of a noninvasive treatment.

The delayed activation of proMMPs and the relatively low cleavage efficiency of MMPs compared to ADAMTS5 (aggrecanase) explains the minor contribution of the MMP enzymes to aggrecan catabolism in vivo.
Title: MMPs are less efficient than ADAMTS5 in cleaving aggrecan core protein.

Self-adhesion between highly negatively charged aggrecan macromolecules extracted from bovine cartilage extracellular matrix, was investigated.
Title: Single-molecule force spectroscopy of cartilage aggrecan self-adhesion.

aggrecanase activity (a) is responsible for early TNFalpha-dependent aggrecan cleavage and GAG release in the meniscus and (b) might be involved in meniscal degeneration.
Title: Tumor necrosis factor alpha-dependent aggrecan cleavage and release of glycosaminoglycans in the meniscus is mediated by nitrous oxide-independent aggrecanase activity in vitro.

The following ACAN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACAN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa228203	XM_024981589.1 Latest version!	Bos taurus aggrecan (ACAN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa228204	XM_024981590.1 Latest version!	Bos taurus aggrecan (ACAN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa44496	NM_173981.2 Latest version!	Bos taurus aggrecan (ACAN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa30830	XM_005221803.3 Latest version!	Bos taurus aggrecan (ACAN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OBa30830	XM_024981588.1 Latest version!	Bos taurus aggrecan (ACAN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACAN ( XM_024981589.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837357.1 Bos taurus aggrecan (ACAN), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa228203
Clone ID Related Accession (Same CDS sequence)	XM_024981589.1
Accession Version	XM_024981589.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	aggrecan core protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTT TACTCTTGGT GTTTGTGACT CTGAGGGTCA TCACAGCAGC CATCTCAGTA 
GAAGTTTCAG AACCTGACAA CTCGCTGAGT GTCAGCATCC CTGAACCGTC CCCGCTGCGG 
GTCCTCCTGG GGAGCTCCCT CACCATCCCC TGCTACTTCA TCGACCCCAT GCACCCCGTG 
ACCACCGCCC CCTCCACCGC CCCCCTTGCC CCAAGAATCA AGTGGAGCCG CATTTCCAAG 
GAGAAGGAGG TGGTGCTGCT GGTGGCCACT GAAGGGCGGG TGCGGGTCAA CAGCGCCTAC 
CAAGACAAGG TCACGCTGCC CAACTACCCC GCCATCCCCA GCGACGCCAC CCTGGAAATC 
CAGAACATGC GCTCCAATGA CTCCGGGATC TATCGCTGCG AGGTGATGCA CGGCATCGAG 
GACAGCCAGG CCACCCTAGA GGTCGTGGTA AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCCACGCGCT ACACCCTGGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGCGCC 
ATCATCGCCA CGCCCGAGCA GCTGCAGGCT GCCTATGAGG ACGGCTTCCA CCAGTGCGAC 
GCCGGCTGGC TGGCCGATCA GACTGTGAGG TATCCCATCC ACACGCCGAG GGAAGGTTGC 
TATGGAGACA AGGACGAGTT TCCCGGCGTG AGAACCTACG GCATCCGGGA CACCAACGAA 
ACCTATGACG TGTACTGCTT CGCGGAGGAG ATGGAGGGCG AGGTCTTCTA TGCAACATCC 
CCGGAGAAGT TCACCTTCCA AGAGGCAGCC AACGAGTGCC GGCGGCTGGG CGCCCGGCTG 
GCCACCACGG GCCAACTCTA CCTGGCCTGG CAGGGTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCTG ACCGCAGCGT GCGATACCCC ATCTCCAAGG CCCGGCCTAA CTGCGGGGGC 
AACCTCCTGG GAGTGAGGAC CGTCTACCTG CACGCCAACC AGACGGGCTA CCCTGACCCT 
TCATCCCGCT ATGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAGC 
TTTTTCGGGG TGGGCGGTGA GGAGGACATC ACCATCCAGA CGGTGACCTG GCCTGACGTG 
GAGCTGCCCC TGCCCCGAAA TATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTCACG 
GCAAAGCCCG ACTTTGAAGT CTCCCCCACC GCCCCGGAAC CCGAGGAGCC TTTCACGTTT 
GTCCCTGAAG TAAGGGCCAC TGCATTCCCC GAAGTAGAGA ACAGGACTGA AGAGGCCACC 
CGGCCCTGGG CCTTTCCCAG AGAGTCCACC CCCGGCCTGG GAGCCCCCAC GGCCTTCACC 
AGCGAGGACC TCGTCGTGCA GGTGACCTTA GCCCCAGGTG CGGCTGAGGT CCCTGGGCAG 
CCACGACTGC CAGGGGGAGT CGTGTTCCAC TACCGCCCAG GCTCCTCCCG CTACTCGCTG 
ACCTTTGAGG AGGCCAAGCA GGCCTGCCTG CGCACCGGGG CCATCATCGC CTCGCCCGAG 
CAGCTCCAGG CCGCCTATGA AGCAGGCTAC GAGCAGTGTG ACGCCGGCTG GCTGCAGGAC 
CAGACAGTCA GATACCCCAT TGTGAGCCCG CGGACCCCCT GTGTGGGTGA CAAGGACAGC 
AGCCCGGGGG TCCGGACCTA CGGCGTGCGG CCACCATCAG AAACCTACGA TGTCTACTGC 
TACGTGGACA GACTCGAGGG GGAGGTGTTC TTCGCCACAC GCCTGGAGCA GTTCACCTTC 
TGGGAAGCCC AGGAGTTCTG TGAATCCCAA AACGCCACTC TGGCCACCAC AGGCCAGCTC 
TATGCTGCCT GGAGCCGTGG TCTGGACAAG TGCTACGCCG GCTGGCTGGC TGACGGCAGC 
CTCCGCTACC CCATCGTCAC CCCAAGGCCC GCCTGTGGCG GGGACAAACC GGGCGTGAGA 
ACCGTCTACC TCTACCCCAA CCAGACGGGC CTCCTGGATC CGCTGTCCCG GCACCACGCC 
TTCTGCTTCC GAGGTGTTTC AGCGGCGCCC TCTCCAGAAG AAGAGGAGGG TAGTGCACCC 
ACAGCAGGCC CTGACGTGGA GGAGTGGATG GTGACACAAG TGGGGCCTGG CGTGGCTGCT 
GTCCCCATCG GGGAGGAGAC GACTGCAATC CCAGGCTTCA CCGTTGAGCC AGAAAACAAG 
ACGGAATGGG AACTTGCCTA CACCCCAGCG GGCACTTTGC CACTACCAGG GATCCCTCCT 
ACATGGCCTC CCACTGGAGA AGCAACAGAG GAGCACACAG AAGGCCCTTC TGCAACGGAA 
GTGCCCTCAG CCTCAGAGAA GCCGTTCCCT TCAGAGGAAC CATTCCCCCC AGAGGAACCA 
TTCCCCTCAG AGAAACCATT CCCCCCAGAG GAACTGTTCC CCTCAGAGAA GCCATTCCCC 
TCAGAAAAGC CGTTCCCCTC AGAGGAACCG TTCCCCTCAG AAAAACCATT CCCCCCAGAG 
GAACTGTTCC CCTCAGAGAA GCCAATCCCC TCAGAGGAGC CATTCCCCTC AGAGGAGCCA 
TTCCCCTCAG AGAAGCCATT CCCCCCAGAG GAACCATTCC CCTCAGAGAA ACCAATCCCC 
TCAGAGGAGC CTTTCCCCTC AGAGAAGCCA TTCCCTTCAG AGGAGCCATT CCCCTCAGAG 
GAGCCATCCA CACTTTCAGC CCCAGTGCCC AGCAGGACTG AGCTGCCAAG CTCTGGGGAG 
GTGTCTGGGG TGCCCGAAAT CAGTGGTGAC TTCACAGGCA GTGGAGAAAT TTCAGGACAC 
CTAGACTTCA GTGGGCAGCC CTCAGGGGAA AGTGCAAGTG GACTGCCCTC TGAAGACCTT 
GACTCCAGTG GGCTCACCTC TACAGTGGGC TCAGGCCTGC CTGTGGAAAG TGGACTGCCT 
TCAGGGGAAG AAGAGAGAAT TACATGGACC AGTGCTCCTA AAGTTGACAG GTTGCCCTCT 
GGGGGTGAGG GCCCAGAAGT TTCTGGAGTA GAGGATATCA GTGGACTTCC TTCTGGAGGA 
GAGGTTCATC TAGAGATCTC TGCCTCTGGA GTAGAGGACA TCAGTGGACT TCCTTCTGGA 
GGAGAGGTTC ATCTAGAGAT CTCTGCCTCT GGAGTAGAGG ACCTAAGTAG AATTCCTTCT 
GGAGAAGGTC CAGAAATCTC TGCCTCTGGA GTAGAGGACA TCAGTGGACT TCCTTCTGGA 
GAGGAAGGTC ATCTAGAGAT CTCTGCCTCT GGAGTAGAAG ACCTCAGTGG AATTCCTTCT 
GGAGAAGGTC CAGAAGTCTC TGCTTCTGGA GTAGAGGACC TTATTGGACT TCCTTCTGGA 
GAAGGTCCAG AAGTCTCTGC TTCTGGAGTA GAGGACCTCA GTAGACTTCC TTCTGGAGAA 
GGTCCAGAAG TCTCTGCTTC TGGGGTAGAG GACCTTAGTG GACTTCCTTC TGGAGAAGGT 
CCAGAAGTCT CTGTTTCTGG AGTAGAGGAT CTCAGCAGAC TTCCTTCTGG AGAAGGTCCA 
GAAGTCTCTG CTTCTGGAGT AGAGGACCTC AGTAGACTTC CTTCTGGAGA AGGTCCAGAA 
ATCTCTGTGT CTGGAGTAGA GGACATCAGT ATACTTCCTT CTGGAGAAGG TCCAGAAGTC 
TCTGCCTCTG GAGTAGAGGA CCTCAGTGTT CTTCCTTCTG GAGAAGGTCA TCTAGAGATC 
TCTACCTCTG GAGTAGAGGA CCTCAGTGTT CTTCCTTCTG GTGAAGGTCA TCTAGAGATC 
TCTGCCTCTG GAGTAGAGGA CATCAGTAGA CTTCCTTCTG GAGAAGGTCC AGAAGTCTCT 
GCCTCTGGAG TAGAGGACCT CAGTGTTCTT CCTTCTGGAG AAGATCATCT AGAGATCTCT 
GCCTCTGGAG TAGAGGACCT CGGTGTTCTT CCTTCTGGAG AAGATCATCT AGAGATCTCT 
GCCTCTGGAG TAGAGGACAT CAGTAGACTT CCTTCTGGAG AAGGTCCAGA AGTCTCTGCC 
TCTGGAGTAG AGGACCTCAG TGTTCTTCCT TCTGGAGAAG GTCATCTAGA GATCTCTGCC 
TCTGGAGTAG AGGACCTCAG TAGACTTCCT TCTGGAGGAG AGGATCATCT AGAGACTTCT 
GCTTCTGGAG TAGGGGACCT TAGTGGACTT CCTTCTGGAA GGGAAGGTCT GGAGATCTCT 
GCTTCTGGAG CTGGGGATCT TAGTGGGTTG ACTTCTGGAA AAGAAGACTT GACTGGGTCA 
GCTTCTGGAG CCTTGGACCT TGGCAGAATA CCTTCTGTAA CTCTAGGAAG TGGGCAAGCT 
CCAGAAGCAA GTGGTCTTCC TTCTGGATTT AGTGGTGAGT ATTCTGGGGT GGACCTTGAA 
AGTGGCCCAT CCTCTGGCCT TCCTGATTTC AGTGGACTTC CATCTGGGTT CCCAACTGTC 
TCCCTAGTGG ATACTACATT GGTGGAAGTC GTCACAGCCA CCACAGCAGG TGAACTAGAA 
GGAAGGGGAA CCATTGACAT CAGCGGTGCT GGAGAAACAT CTGGGCTGCC CTTCAGTGAG 
TTGGACATTA GTGGAGGAGC AAGCGGACTC TCTTCAGGAG CTGAACTCAG TGGCCAAGCA 
TCTGGGTCTC CTGACATCAG TGGGGAAACC TCTGGACTCT TTGGTGTCAG TGGACAGCCC 
TCAGGGTTTC CTGACATTAG TGGGGAAACT TCCGGGCTTC TTGAGGTCAG TGGGCAGCCA 
TCAGGGTTTT ATGGAGAAAT ATCTGGCGTG ACTGAGCTTA GCGGACTGGC CTCTGGACAA 
CCAGAAATCA GTGGAGAAGC TTCTGGAATT CTTTCTGGCC TTGGTCCACC ATTCGGCATA 
ACCGACCTGA GCGGAGAAGC ACCCGGGATC CCTGATCTCA GTGGGCAACC ATCGGGCTTG 
CCAGAGTTCA GTGGGACAAC ATCCGGGATC CCTGACCTGG TTTCCAGTGC TGTGAGTGGC 
AGTGGTGAAT CTTCTGGCAT TACGTTCGTG GACACCAGTT TGGTTGAAGT GACCCCAACG 
ACATTTAAAG AAGAAGAAGG CTTAGGATCT GTGGAACTCA GTGGCCTCCC TTCAGGAGAG 
GTGGGTGTCT CAGGCACATC TGGGCTAGCG GATGTCAGTG GACTGTCTTC TGGAGCAATT 
GACTCCAGTG GGTTTACGTC CCAGCCTCCA GAATTCAGTG GCCTGCCAAG CGGAGTAACT 
GAGGTCAGTG GAGAAGCCTC CGGAGCTGAA AGTGGGAGCA GCCTGCCCTC CGGAGCATAT 
GACAGCAGTG GACTTCCGTC TGGTTTCCCC ACTGTCTCTT TTGTAGACAG GACTTTGGTG 
GAATCTGTAA CCCAGGCTCC AACTGCTCAA GAAGCAGGAG AAGGGCCTTC AGGCATTCTG 
GAACTTAGCG GTGCGCCTTC TGGAGCACCA GACATGTCTG GAGACCATTT GGGATCTTTG 
GACCAAAGTG GGCTTCAGTC TGGACTAGTG GAGCCCAGTG GGGAGCCCGC AAGTACTCCA 
TATTTTAGTG GGGACTTTTC TGGCACCACT GATGTAAGTG GGGAATCCTC TGCAGCCACG 
AGCACCAGTG GGGAGGCCTC CGGACTTCCA GAAGTTACGT TAATCACATC TGAGTTGGTG 
GAGGGAGTTA CTGAACCAAC CGTTTCCCAG GAACTGGGCC AGAGACCCCC CGTAACATAC 
ACCCCCCAGC TTTTTGAATC CAGTGGTGAA GCCTCTGCAT CTGGGGATGT GCCAAGGTTC 
CCTGGATCGG GGGTAGAAGT GTCATCAGTC CCAGAATCCA GCGGTGAGAC GTCAGCCTAT 
CCCGAGGCTG AGGTGGGAGC ATCTGCTGCC CCCGAGGCAA GCGGAGGAGC TTCCGGGTCC 
CCTAACCTGA GTGAAACCAC CTCCACCTTC CATGAAGCTG ATCTGGAGGG AACCTCAGGC 
CTGGGAGTGA GTGGCAGCCC CTCAGCCTTT CCAGAAGGCC CCACGGAGGG CTTGGCCACC 
CCGGAAGTGA GTGGAGAGTC AACCACTGCC TTTGACGTGA GCGTAGAGGC ATCAGGCTCA 
CCTTCTGCCA CTCCCCTGGC TTCTGGAGAC AGGACTGACA CCAGCGGAGA TCTGTCTGGC 
CACACCTCGG GGCTGGATAT TGTCATCAGC ACCACCATCC CAGAATCCGA GTGGACTCAG 
CAGACCCAGC GCCCTGCAGA GGCGCGTCTA GAAATCGAAT CCTCAAGCCC TGTGCACTCA 
GGAGAAGAGA GCCAAACAGC CGACACAGCC ACCTCCCCGA CTGATGCTTC TATCCCAGCC 
TCCGCAGGAG GGACAGATGA TTCAGAGGCA ACCACAACAG ACCAGAAGCT GTGCGAGGAG 
GGCTGGACCA AGTTCCAGGG CCACTGTTAC CGCCACTTCC CCGACCGGGC AACCTGGGTG 
GACGCTGAGA GCCAGTGCCG GAAGCAGCAG TCACACCTGA GCAGCATCGT CACCCCCGAG 
GAGCAGGAGT TTGTCAACAA CAATGCCCAG GACTACCAGT GGATCGGCCT GAACGACAAG 
ACCATCGAAG GGGACTTCCG CTGGTCAGAT GGACACTCCT TGCAATTTGA GAACTGGCGC 
CCCAACCAGC CTGACAACTT CTTTGCCACT GGGGAGGACT GTGTGGTGAT GATCTGGCAT 
GAGAAGGGCG AGTGGAACGA TGTCCCATGT AATTACCAGC TACCCTTCAC CTGTAAAAAG 
GGCACAGTGG CCTGCGGAGA GCCCCCCGTG GTGGAGCATG CCAGAATCTT CGGACAGAAG 
AAGGACCGAT ACGAGATCAA TGCCCTGGTG CGGTACCAGT GCACCGAGGG CTTCATCCAG 
CGCCATGTGC CCACCATCCG GTGCCAGCCC AGCGGGCACT GGGAGGAGCC TCGGATCACC 
TGCACAGACC CCGCTACCTA CAAGCGCAGA CTACAGAAGC GGAGCTCACG GCCCCTGAGG 
AGGAGCCACC CCAGCACGGC CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837357.1
CDS	11..6997
Translation	MTTLLLVFVTLRVITAAISVEVSEPDNSLSVSIPEPSPLRVLLGSSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRISKEKEVVLLVATEGRVRVNSAYQDKVTLPNYPAIPSDATLEIQNMRSNDSGIYRCEVMHGIEDSQATLEVVVKGIVFHYRAISTRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPREGCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRLGARLATTGQLYLAWQGGMDMCSAGWLADRSVRYPISKARPNCGGNLLGVRTVYLHANQTGYPDPSSRYDAICYTGEDFVDIPESFFGVGGEEDITIQTVTWPDVELPLPRNITEGEARGSVILTAKPDFEVSPTAPEPEEPFTFVPEVRATAFPEVENRTEEATRPWAFPRESTPGLGAPTAFTSEDLVVQVTLAPGAAEVPGQPRLPGGVVFHYRPGSSRYSLTFEEAKQACLRTGAIIASPEQLQAAYEAGYEQCDAGWLQDQTVRYPIVSPRTPCVGDKDSSPGVRTYGVRPPSETYDVYCYVDRLEGEVFFATRLEQFTFWEAQEFCESQNATLATTGQLYAAWSRGLDKCYAGWLADGSLRYPIVTPRPACGGDKPGVRTVYLYPNQTGLLDPLSRHHAFCFRGVSAAPSPEEEEGSAPTAGPDVEEWMVTQVGPGVAAVPIGEETTAIPGFTVEPENKTEWELAYTPAGTLPLPGIPPTWPPTGEATEEHTEGPSATEVPSASEKPFPSEEPFPPEEPFPSEKPFPPEELFPSEKPFPSEKPFPSEEPFPSEKPFPPEELFPSEKPIPSEEPFPSEEPFPSEKPFPPEEPFPSEKPIPSEEPFPSEKPFPSEEPFPSEEPSTLSAPVPSRTELPSSGEVSGVPEISGDFTGSGEISGHLDFSGQPSGESASGLPSEDLDSSGLTSTVGSGLPVESGLPSGEEERITWTSAPKVDRLPSGGEGPEVSGVEDISGLPSGGEVHLEISASGVEDISGLPSGGEVHLEISASGVEDLSRIPSGEGPEISASGVEDISGLPSGEEGHLEISASGVEDLSGIPSGEGPEVSASGVEDLIGLPSGEGPEVSASGVEDLSRLPSGEGPEVSASGVEDLSGLPSGEGPEVSVSGVEDLSRLPSGEGPEVSASGVEDLSRLPSGEGPEISVSGVEDISILPSGEGPEVSASGVEDLSVLPSGEGHLEISTSGVEDLSVLPSGEGHLEISASGVEDISRLPSGEGPEVSASGVEDLSVLPSGEDHLEISASGVEDLGVLPSGEDHLEISASGVEDISRLPSGEGPEVSASGVEDLSVLPSGEGHLEISASGVEDLSRLPSGGEDHLETSASGVGDLSGLPSGREGLEISASGAGDLSGLTSGKEDLTGSASGALDLGRIPSVTLGSGQAPEASGLPSGFSGEYSGVDLESGPSSGLPDFSGLPSGFPTVSLVDTTLVEVVTATTAGELEGRGTIDISGAGETSGLPFSELDISGGASGLSSGAELSGQASGSPDISGETSGLFGVSGQPSGFPDISGETSGLLEVSGQPSGFYGEISGVTELSGLASGQPEISGEASGILSGLGPPFGITDLSGEAPGIPDLSGQPSGLPEFSGTTSGIPDLVSSAVSGSGESSGITFVDTSLVEVTPTTFKEEEGLGSVELSGLPSGEVGVSGTSGLADVSGLSSGAIDSSGFTSQPPEFSGLPSGVTEVSGEASGAESGSSLPSGAYDSSGLPSGFPTVSFVDRTLVESVTQAPTAQEAGEGPSGILELSGAPSGAPDMSGDHLGSLDQSGLQSGLVEPSGEPASTPYFSGDFSGTTDVSGESSAATSTSGEASGLPEVTLITSELVEGVTEPTVSQELGQRPPVTYTPQLFESSGEASASGDVPRFPGSGVEVSSVPESSGETSAYPEAEVGASAAPEASGGASGSPNLSETTSTFHEADLEGTSGLGVSGSPSAFPEGPTEGLATPEVSGESTTAFDVSVEASGSPSATPLASGDRTDTSGDLSGHTSGLDIVISTTIPESEWTQQTQRPAEARLEIESSSPVHSGEESQTADTATSPTDASIPASAGGTDDSEATTTDQKLCEEGWTKFQGHCYRHFPDRATWVDAESQCRKQQSHLSSIVTPEEQEFVNNNAQDYQWIGLNDKTIEGDFRWSDGHSLQFENWRPNQPDNFFATGEDCVVMIWHEKGEWNDVPCNYQLPFTCKKGTVACGEPPVVEHARIFGQKKDRYEINALVRYQCTEGFIQRHVPTIRCQPSGHWEEPRITCTDPATYKRRLQKRSSRPLRRSHPSTAH

Target ORF information:

RefSeq Version	XM_024981589.1
Organism	Bos taurus(cattle)
Definition	Bos taurus aggrecan (ACAN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981589.1

ORF Insert Sequence:

ATGACCACTT TACTCTTGGT GTTTGTGACT CTGAGGGTCA TCACAGCAGC CATCTCAGTA 
GAAGTTTCAG AACCTGACAA CTCGCTGAGT GTCAGCATCC CTGAACCGTC CCCGCTGCGG 
GTCCTCCTGG GGAGCTCCCT CACCATCCCC TGCTACTTCA TCGACCCCAT GCACCCCGTG 
ACCACCGCCC CCTCCACCGC CCCCCTTGCC CCAAGAATCA AGTGGAGCCG CATTTCCAAG 
GAGAAGGAGG TGGTGCTGCT GGTGGCCACT GAAGGGCGGG TGCGGGTCAA CAGCGCCTAC 
CAAGACAAGG TCACGCTGCC CAACTACCCC GCCATCCCCA GCGACGCCAC CCTGGAAATC 
CAGAACATGC GCTCCAATGA CTCCGGGATC TATCGCTGCG AGGTGATGCA CGGCATCGAG 
GACAGCCAGG CCACCCTAGA GGTCGTGGTA AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCCACGCGCT ACACCCTGGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGCGCC 
ATCATCGCCA CGCCCGAGCA GCTGCAGGCT GCCTATGAGG ACGGCTTCCA CCAGTGCGAC 
GCCGGCTGGC TGGCCGATCA GACTGTGAGG TATCCCATCC ACACGCCGAG GGAAGGTTGC 
TATGGAGACA AGGACGAGTT TCCCGGCGTG AGAACCTACG GCATCCGGGA CACCAACGAA 
ACCTATGACG TGTACTGCTT CGCGGAGGAG ATGGAGGGCG AGGTCTTCTA TGCAACATCC 
CCGGAGAAGT TCACCTTCCA AGAGGCAGCC AACGAGTGCC GGCGGCTGGG CGCCCGGCTG 
GCCACCACGG GCCAACTCTA CCTGGCCTGG CAGGGTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCTG ACCGCAGCGT GCGATACCCC ATCTCCAAGG CCCGGCCTAA CTGCGGGGGC 
AACCTCCTGG GAGTGAGGAC CGTCTACCTG CACGCCAACC AGACGGGCTA CCCTGACCCT 
TCATCCCGCT ATGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAGC 
TTTTTCGGGG TGGGCGGTGA GGAGGACATC ACCATCCAGA CGGTGACCTG GCCTGACGTG 
GAGCTGCCCC TGCCCCGAAA TATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTCACG 
GCAAAGCCCG ACTTTGAAGT CTCCCCCACC GCCCCGGAAC CCGAGGAGCC TTTCACGTTT 
GTCCCTGAAG TAAGGGCCAC TGCATTCCCC GAAGTAGAGA ACAGGACTGA AGAGGCCACC 
CGGCCCTGGG CCTTTCCCAG AGAGTCCACC CCCGGCCTGG GAGCCCCCAC GGCCTTCACC 
AGCGAGGACC TCGTCGTGCA GGTGACCTTA GCCCCAGGTG CGGCTGAGGT CCCTGGGCAG 
CCACGACTGC CAGGGGGAGT CGTGTTCCAC TACCGCCCAG GCTCCTCCCG CTACTCGCTG 
ACCTTTGAGG AGGCCAAGCA GGCCTGCCTG CGCACCGGGG CCATCATCGC CTCGCCCGAG 
CAGCTCCAGG CCGCCTATGA AGCAGGCTAC GAGCAGTGTG ACGCCGGCTG GCTGCAGGAC 
CAGACAGTCA GATACCCCAT TGTGAGCCCG CGGACCCCCT GTGTGGGTGA CAAGGACAGC 
AGCCCGGGGG TCCGGACCTA CGGCGTGCGG CCACCATCAG AAACCTACGA TGTCTACTGC 
TACGTGGACA GACTCGAGGG GGAGGTGTTC TTCGCCACAC GCCTGGAGCA GTTCACCTTC 
TGGGAAGCCC AGGAGTTCTG TGAATCCCAA AACGCCACTC TGGCCACCAC AGGCCAGCTC 
TATGCTGCCT GGAGCCGTGG TCTGGACAAG TGCTACGCCG GCTGGCTGGC TGACGGCAGC 
CTCCGCTACC CCATCGTCAC CCCAAGGCCC GCCTGTGGCG GGGACAAACC GGGCGTGAGA 
ACCGTCTACC TCTACCCCAA CCAGACGGGC CTCCTGGATC CGCTGTCCCG GCACCACGCC 
TTCTGCTTCC GAGGTGTTTC AGCGGCGCCC TCTCCAGAAG AAGAGGAGGG TAGTGCACCC 
ACAGCAGGCC CTGACGTGGA GGAGTGGATG GTGACACAAG TGGGGCCTGG CGTGGCTGCT 
GTCCCCATCG GGGAGGAGAC GACTGCAATC CCAGGCTTCA CCGTTGAGCC AGAAAACAAG 
ACGGAATGGG AACTTGCCTA CACCCCAGCG GGCACTTTGC CACTACCAGG GATCCCTCCT 
ACATGGCCTC CCACTGGAGA AGCAACAGAG GAGCACACAG AAGGCCCTTC TGCAACGGAA 
GTGCCCTCAG CCTCAGAGAA GCCGTTCCCT TCAGAGGAAC CATTCCCCCC AGAGGAACCA 
TTCCCCTCAG AGAAACCATT CCCCCCAGAG GAACTGTTCC CCTCAGAGAA GCCATTCCCC 
TCAGAAAAGC CGTTCCCCTC AGAGGAACCG TTCCCCTCAG AAAAACCATT CCCCCCAGAG 
GAACTGTTCC CCTCAGAGAA GCCAATCCCC TCAGAGGAGC CATTCCCCTC AGAGGAGCCA 
TTCCCCTCAG AGAAGCCATT CCCCCCAGAG GAACCATTCC CCTCAGAGAA ACCAATCCCC 
TCAGAGGAGC CTTTCCCCTC AGAGAAGCCA TTCCCTTCAG AGGAGCCATT CCCCTCAGAG 
GAGCCATCCA CACTTTCAGC CCCAGTGCCC AGCAGGACTG AGCTGCCAAG CTCTGGGGAG 
GTGTCTGGGG TGCCCGAAAT CAGTGGTGAC TTCACAGGCA GTGGAGAAAT TTCAGGACAC 
CTAGACTTCA GTGGGCAGCC CTCAGGGGAA AGTGCAAGTG GACTGCCCTC TGAAGACCTT 
GACTCCAGTG GGCTCACCTC TACAGTGGGC TCAGGCCTGC CTGTGGAAAG TGGACTGCCT 
TCAGGGGAAG AAGAGAGAAT TACATGGACC AGTGCTCCTA AAGTTGACAG GTTGCCCTCT 
GGGGGTGAGG GCCCAGAAGT TTCTGGAGTA GAGGATATCA GTGGACTTCC TTCTGGAGGA 
GAGGTTCATC TAGAGATCTC TGCCTCTGGA GTAGAGGACA TCAGTGGACT TCCTTCTGGA 
GGAGAGGTTC ATCTAGAGAT CTCTGCCTCT GGAGTAGAGG ACCTAAGTAG AATTCCTTCT 
GGAGAAGGTC CAGAAATCTC TGCCTCTGGA GTAGAGGACA TCAGTGGACT TCCTTCTGGA 
GAGGAAGGTC ATCTAGAGAT CTCTGCCTCT GGAGTAGAAG ACCTCAGTGG AATTCCTTCT 
GGAGAAGGTC CAGAAGTCTC TGCTTCTGGA GTAGAGGACC TTATTGGACT TCCTTCTGGA 
GAAGGTCCAG AAGTCTCTGC TTCTGGAGTA GAGGACCTCA GTAGACTTCC TTCTGGAGAA 
GGTCCAGAAG TCTCTGCTTC TGGGGTAGAG GACCTTAGTG GACTTCCTTC TGGAGAAGGT 
CCAGAAGTCT CTGTTTCTGG AGTAGAGGAT CTCAGCAGAC TTCCTTCTGG AGAAGGTCCA 
GAAGTCTCTG CTTCTGGAGT AGAGGACCTC AGTAGACTTC CTTCTGGAGA AGGTCCAGAA 
ATCTCTGTGT CTGGAGTAGA GGACATCAGT ATACTTCCTT CTGGAGAAGG TCCAGAAGTC 
TCTGCCTCTG GAGTAGAGGA CCTCAGTGTT CTTCCTTCTG GAGAAGGTCA TCTAGAGATC 
TCTACCTCTG GAGTAGAGGA CCTCAGTGTT CTTCCTTCTG GTGAAGGTCA TCTAGAGATC 
TCTGCCTCTG GAGTAGAGGA CATCAGTAGA CTTCCTTCTG GAGAAGGTCC AGAAGTCTCT 
GCCTCTGGAG TAGAGGACCT CAGTGTTCTT CCTTCTGGAG AAGATCATCT AGAGATCTCT 
GCCTCTGGAG TAGAGGACCT CGGTGTTCTT CCTTCTGGAG AAGATCATCT AGAGATCTCT 
GCCTCTGGAG TAGAGGACAT CAGTAGACTT CCTTCTGGAG AAGGTCCAGA AGTCTCTGCC 
TCTGGAGTAG AGGACCTCAG TGTTCTTCCT TCTGGAGAAG GTCATCTAGA GATCTCTGCC 
TCTGGAGTAG AGGACCTCAG TAGACTTCCT TCTGGAGGAG AGGATCATCT AGAGACTTCT 
GCTTCTGGAG TAGGGGACCT TAGTGGACTT CCTTCTGGAA GGGAAGGTCT GGAGATCTCT 
GCTTCTGGAG CTGGGGATCT TAGTGGGTTG ACTTCTGGAA AAGAAGACTT GACTGGGTCA 
GCTTCTGGAG CCTTGGACCT TGGCAGAATA CCTTCTGTAA CTCTAGGAAG TGGGCAAGCT 
CCAGAAGCAA GTGGTCTTCC TTCTGGATTT AGTGGTGAGT ATTCTGGGGT GGACCTTGAA 
AGTGGCCCAT CCTCTGGCCT TCCTGATTTC AGTGGACTTC CATCTGGGTT CCCAACTGTC 
TCCCTAGTGG ATACTACATT GGTGGAAGTC GTCACAGCCA CCACAGCAGG TGAACTAGAA 
GGAAGGGGAA CCATTGACAT CAGCGGTGCT GGAGAAACAT CTGGGCTGCC CTTCAGTGAG 
TTGGACATTA GTGGAGGAGC AAGCGGACTC TCTTCAGGAG CTGAACTCAG TGGCCAAGCA 
TCTGGGTCTC CTGACATCAG TGGGGAAACC TCTGGACTCT TTGGTGTCAG TGGACAGCCC 
TCAGGGTTTC CTGACATTAG TGGGGAAACT TCCGGGCTTC TTGAGGTCAG TGGGCAGCCA 
TCAGGGTTTT ATGGAGAAAT ATCTGGCGTG ACTGAGCTTA GCGGACTGGC CTCTGGACAA 
CCAGAAATCA GTGGAGAAGC TTCTGGAATT CTTTCTGGCC TTGGTCCACC ATTCGGCATA 
ACCGACCTGA GCGGAGAAGC ACCCGGGATC CCTGATCTCA GTGGGCAACC ATCGGGCTTG 
CCAGAGTTCA GTGGGACAAC ATCCGGGATC CCTGACCTGG TTTCCAGTGC TGTGAGTGGC 
AGTGGTGAAT CTTCTGGCAT TACGTTCGTG GACACCAGTT TGGTTGAAGT GACCCCAACG 
ACATTTAAAG AAGAAGAAGG CTTAGGATCT GTGGAACTCA GTGGCCTCCC TTCAGGAGAG 
GTGGGTGTCT CAGGCACATC TGGGCTAGCG GATGTCAGTG GACTGTCTTC TGGAGCAATT 
GACTCCAGTG GGTTTACGTC CCAGCCTCCA GAATTCAGTG GCCTGCCAAG CGGAGTAACT 
GAGGTCAGTG GAGAAGCCTC CGGAGCTGAA AGTGGGAGCA GCCTGCCCTC CGGAGCATAT 
GACAGCAGTG GACTTCCGTC TGGTTTCCCC ACTGTCTCTT TTGTAGACAG GACTTTGGTG 
GAATCTGTAA CCCAGGCTCC AACTGCTCAA GAAGCAGGAG AAGGGCCTTC AGGCATTCTG 
GAACTTAGCG GTGCGCCTTC TGGAGCACCA GACATGTCTG GAGACCATTT GGGATCTTTG 
GACCAAAGTG GGCTTCAGTC TGGACTAGTG GAGCCCAGTG GGGAGCCCGC AAGTACTCCA 
TATTTTAGTG GGGACTTTTC TGGCACCACT GATGTAAGTG GGGAATCCTC TGCAGCCACG 
AGCACCAGTG GGGAGGCCTC CGGACTTCCA GAAGTTACGT TAATCACATC TGAGTTGGTG 
GAGGGAGTTA CTGAACCAAC CGTTTCCCAG GAACTGGGCC AGAGACCCCC CGTAACATAC 
ACCCCCCAGC TTTTTGAATC CAGTGGTGAA GCCTCTGCAT CTGGGGATGT GCCAAGGTTC 
CCTGGATCGG GGGTAGAAGT GTCATCAGTC CCAGAATCCA GCGGTGAGAC GTCAGCCTAT 
CCCGAGGCTG AGGTGGGAGC ATCTGCTGCC CCCGAGGCAA GCGGAGGAGC TTCCGGGTCC 
CCTAACCTGA GTGAAACCAC CTCCACCTTC CATGAAGCTG ATCTGGAGGG AACCTCAGGC 
CTGGGAGTGA GTGGCAGCCC CTCAGCCTTT CCAGAAGGCC CCACGGAGGG CTTGGCCACC 
CCGGAAGTGA GTGGAGAGTC AACCACTGCC TTTGACGTGA GCGTAGAGGC ATCAGGCTCA 
CCTTCTGCCA CTCCCCTGGC TTCTGGAGAC AGGACTGACA CCAGCGGAGA TCTGTCTGGC 
CACACCTCGG GGCTGGATAT TGTCATCAGC ACCACCATCC CAGAATCCGA GTGGACTCAG 
CAGACCCAGC GCCCTGCAGA GGCGCGTCTA GAAATCGAAT CCTCAAGCCC TGTGCACTCA 
GGAGAAGAGA GCCAAACAGC CGACACAGCC ACCTCCCCGA CTGATGCTTC TATCCCAGCC 
TCCGCAGGAG GGACAGATGA TTCAGAGGCA ACCACAACAG ACCAGAAGCT GTGCGAGGAG 
GGCTGGACCA AGTTCCAGGG CCACTGTTAC CGCCACTTCC CCGACCGGGC AACCTGGGTG 
GACGCTGAGA GCCAGTGCCG GAAGCAGCAG TCACACCTGA GCAGCATCGT CACCCCCGAG 
GAGCAGGAGT TTGTCAACAA CAATGCCCAG GACTACCAGT GGATCGGCCT GAACGACAAG 
ACCATCGAAG GGGACTTCCG CTGGTCAGAT GGACACTCCT TGCAATTTGA GAACTGGCGC 
CCCAACCAGC CTGACAACTT CTTTGCCACT GGGGAGGACT GTGTGGTGAT GATCTGGCAT 
GAGAAGGGCG AGTGGAACGA TGTCCCATGT AATTACCAGC TACCCTTCAC CTGTAAAAAG 
GGCACAGTGG CCTGCGGAGA GCCCCCCGTG GTGGAGCATG CCAGAATCTT CGGACAGAAG 
AAGGACCGAT ACGAGATCAA TGCCCTGGTG CGGTACCAGT GCACCGAGGG CTTCATCCAG 
CGCCATGTGC CCACCATCCG GTGCCAGCCC AGCGGGCACT GGGAGGAGCC TCGGATCACC 
TGCACAGACC CCGCTACCTA CAAGCGCAGA CTACAGAAGC GGAGCTCACG GCCCCTGAGG 
AGGAGCCACC CCAGCACGGC CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACAN ( XM_024981590.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837358.1 Bos taurus aggrecan (ACAN), transcript variant X3, mRNA.

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CloneID	OBa228204
Clone ID Related Accession (Same CDS sequence)	XM_024981590.1
Accession Version	XM_024981590.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	aggrecan core protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTT TACTCTTGGT GTTTGTGACT CTGAGGGTCA TCACAGCAGC CATCTCAGTA 
GAAGTTTCAG AACCTGACAA CTCGCTGAGT GTCAGCATCC CTGAACCGTC CCCGCTGCGG 
GTCCTCCTGG GGAGCTCCCT CACCATCCCC TGCTACTTCA TCGACCCCAT GCACCCCGTG 
ACCACCGCCC CCTCCACCGC CCCCCTTGCC CCAAGAATCA AGTGGAGCCG CATTTCCAAG 
GAGAAGGAGG TGGTGCTGCT GGTGGCCACT GAAGGGCGGG TGCGGGTCAA CAGCGCCTAC 
CAAGACAAGG TCACGCTGCC CAACTACCCC GCCATCCCCA GCGACGCCAC CCTGGAAATC 
CAGAACATGC GCTCCAATGA CTCCGGGATC TATCGCTGCG AGGTGATGCA CGGCATCGAG 
GACAGCCAGG CCACCCTAGA GGTCGTGGTA AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCCACGCGCT ACACCCTGGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGCGCC 
ATCATCGCCA CGCCCGAGCA GCTGCAGGCT GCCTATGAGG ACGGCTTCCA CCAGTGCGAC 
GCCGGCTGGC TGGCCGATCA GACTGTGAGG TATCCCATCC ACACGCCGAG GGAAGGTTGC 
TATGGAGACA AGGACGAGTT TCCCGGCGTG AGAACCTACG GCATCCGGGA CACCAACGAA 
ACCTATGACG TGTACTGCTT CGCGGAGGAG ATGGAGGGCG AGGTCTTCTA TGCAACATCC 
CCGGAGAAGT TCACCTTCCA AGAGGCAGCC AACGAGTGCC GGCGGCTGGG CGCCCGGCTG 
GCCACCACGG GCCAACTCTA CCTGGCCTGG CAGGGTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCTG ACCGCAGCGT GCGATACCCC ATCTCCAAGG CCCGGCCTAA CTGCGGGGGC 
AACCTCCTGG GAGTGAGGAC CGTCTACCTG CACGCCAACC AGACGGGCTA CCCTGACCCT 
TCATCCCGCT ATGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAGC 
TTTTTCGGGG TGGGCGGTGA GGAGGACATC ACCATCCAGA CGGTGACCTG GCCTGACGTG 
GAGCTGCCCC TGCCCCGAAA TATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTCACG 
GCAAAGCCCG ACTTTGAAGT CTCCCCCACC GCCCCGGAAC CCGAGGAGCC TTTCACGTTT 
GTCCCTGAAG TAAGGGCCAC TGCATTCCCC GAAGTAGAGA ACAGGACTGA AGAGGCCACC 
CGGCCCTGGG CCTTTCCCAG AGAGTCCACC CCCGGCCTGG GAGCCCCCAC GGCCTTCACC 
AGCGAGGACC TCGTCGTGCA GGTGACCTTA GCCCCAGGTG CGGCTGAGGT CCCTGGGCAG 
CCACGACTGC CAGGGGGAGT CGTGTTCCAC TACCGCCCAG GCTCCTCCCG CTACTCGCTG 
ACCTTTGAGG AGGCCAAGCA GGCCTGCCTG CGCACCGGGG CCATCATCGC CTCGCCCGAG 
CAGCTCCAGG CCGCCTATGA AGCAGGCTAC GAGCAGTGTG ACGCCGGCTG GCTGCAGGAC 
CAGACAGTCA GATACCCCAT TGTGAGCCCG CGGACCCCCT GTGTGGGTGA CAAGGACAGC 
AGCCCGGGGG TCCGGACCTA CGGCGTGCGG CCACCATCAG AAACCTACGA TGTCTACTGC 
TACGTGGACA GACTCGAGGG GGAGGTGTTC TTCGCCACAC GCCTGGAGCA GTTCACCTTC 
TGGGAAGCCC AGGAGTTCTG TGAATCCCAA AACGCCACTC TGGCCACCAC AGGCCAGCTC 
TATGCTGCCT GGAGCCGTGG TCTGGACAAG TGCTACGCCG GCTGGCTGGC TGACGGCAGC 
CTCCGCTACC CCATCGTCAC CCCAAGGCCC GCCTGTGGCG GGGACAAACC GGGCGTGAGA 
ACCGTCTACC TCTACCCCAA CCAGACGGGC CTCCTGGATC CGCTGTCCCG GCACCACGCC 
TTCTGCTTCC GAGGTGTTTC AGCGGCGCCC TCTCCAGAAG AAGAGGAGGG TAGTGCACCC 
ACAGCAGGCC CTGACGTGGA GGAGTGGATG GTGACACAAG TGGGGCCTGG CGTGGCTGCT 
GTCCCCATCG GGGAGGAGAC GACTGCAATC CCAGGCTTCA CCGTTGAGCC AGAAAACAAG 
ACGGAATGGG AACTTGCCTA CACCCCAGCG GGCACTTTGC CACTACCAGG GATCCCTCCT 
ACATGGCCTC CCACTGGAGA AGCAACAGAG GAGCACACAG AAGGCCCTTC TGCAACGGAA 
GTGCCCTCAG CCTCAGAGAA GCCGTTCCCT TCAGAGGAAC CATTCCCCCC AGAGGAACCA 
TTCCCCTCAG AGAAACCATT CCCCCCAGAG GAACTGTTCC CCTCAGAGAA GCCATTCCCC 
TCAGAAAAGC CGTTCCCCTC AGAGGAACCG TTCCCCTCAG AAAAACCATT CCCCCCAGAG 
GAACTGTTCC CCTCAGAGAA GCCAATCCCC TCAGAGGAGC CATTCCCCTC AGAGGAGCCA 
TTCCCCTCAG AGAAGCCATT CCCCCCAGAG GAACCATTCC CCTCAGAGAA ACCAATCCCC 
TCAGAGGAGC CTTTCCCCTC AGAGAAGCCA TTCCCTTCAG AGGAGCCATT CCCCTCAGAG 
GAGCCATCCA CACTTTCAGC CCCAGTGCCC AGCAGGACTG AGCTGCCAAG CTCTGGGGAG 
GTGTCTGGGG TGCCCGAAAT CAGTGGTGAC TTCACAGGCA GTGGAGAAAT TTCAGGACAC 
CTAGACTTCA GTGGGCAGCC CTCAGGGGAA AGTGCAAGTG GACTGCCCTC TGAAGACCTT 
GACTCCAGTG GGCTCACCTC TACAGTGGGC TCAGGCCTGC CTGTGGAAAG TGGACTGCCT 
TCAGGGGAAG AAGAGAGAAT TACATGGACC AGTGCTCCTA AAGTTGACAG GTTGCCCTCT 
GGGGGTGAGG GCCCAGAAGT TTCTGGAGTA GAGGATATCA GTGGACTTCC TTCTGGAGGA 
GAGGTTCATC TAGAGATCTC TGCCTCTGGA GTAGAGGACA TCAGTGGACT TCCTTCTGGA 
GGAGAGGTTC ATCTAGAGAT CTCTGCCTCT GGAGTAGAGG ACCTAAGTAG AATTCCTTCT 
GGAGAAGGTC CAGAAATCTC TGCCTCTGGA GTAGAGGACA TCAGTGGACT TCCTTCTGGA 
GAGGAAGGTC ATCTAGAGAT CTCTGCCTCT GGAGTAGAAG ACCTCAGTGG AATTCCTTCT 
GGAGAAGGTC CAGAAGTCTC TGCTTCTGGA GTAGAGGACC TTATTGGACT TCCTTCTGGA 
GAAGGTCCAG AAGTCTCTGC TTCTGGAGTA GAGGACCTCA GTAGACTTCC TTCTGGAGAA 
GGTCCAGAAG TCTCTGCTTC TGGGGTAGAG GACCTTAGTG GACTTCCTTC TGGAGAAGGT 
CCAGAAGTCT CTGTTTCTGG AGTAGAGGAT CTCAGCAGAC TTCCTTCTGG AGAAGGTCCA 
GAAGTCTCTG CTTCTGGAGT AGAGGACCTC AGTAGACTTC CTTCTGGAGA AGGTCCAGAA 
ATCTCTGTGT CTGGAGTAGA GGACATCAGT ATACTTCCTT CTGGAGAAGG TCCAGAAGTC 
TCTGCCTCTG GAGTAGAGGA CCTCAGTGTT CTTCCTTCTG GAGAAGGTCA TCTAGAGATC 
TCTACCTCTG GAGTAGAGGA CCTCAGTGTT CTTCCTTCTG GTGAAGGTCA TCTAGAGATC 
TCTGCCTCTG GAGTAGAGGA CATCAGTAGA CTTCCTTCTG GAGAAGGTCC AGAAGTCTCT 
GCCTCTGGAG TAGAGGACCT CAGTGTTCTT CCTTCTGGAG AAGATCATCT AGAGATCTCT 
GCCTCTGGAG TAGAGGACCT CGGTGTTCTT CCTTCTGGAG AAGATCATCT AGAGATCTCT 
GCCTCTGGAG TAGAGGACAT CAGTAGACTT CCTTCTGGAG AAGGTCCAGA AGTCTCTGCC 
TCTGGAGTAG AGGACCTCAG TGTTCTTCCT TCTGGAGAAG GTCATCTAGA GATCTCTGCC 
TCTGGAGTAG AGGACCTCAG TAGACTTCCT TCTGGAGGAG AGGATCATCT AGAGACTTCT 
GCTTCTGGAG TAGGGGACCT TAGTGGACTT CCTTCTGGAA GGGAAGGTCT GGAGATCTCT 
GCTTCTGGAG CTGGGGATCT TAGTGGGTTG ACTTCTGGAA AAGAAGACTT GACTGGGTCA 
GCTTCTGGAG CCTTGGACCT TGGCAGAATA CCTTCTGTAA CTCTAGGAAG TGGGCAAGCT 
CCAGAAGCAA GTGGTCTTCC TTCTGGATTT AGTGGTGAGT ATTCTGGGGT GGACCTTGAA 
AGTGGCCCAT CCTCTGGCCT TCCTGATTTC AGTGGACTTC CATCTGGGTT CCCAACTGTC 
TCCCTAGTGG ATACTACATT GGTGGAAGTC GTCACAGCCA CCACAGCAGG TGAACTAGAA 
GGAAGGGGAA CCATTGACAT CAGCGGTGCT GGAGAAACAT CTGGGCTGCC CTTCAGTGAG 
TTGGACATTA GTGGAGGAGC AAGCGGACTC TCTTCAGGAG CTGAACTCAG TGGCCAAGCA 
TCTGGGTCTC CTGACATCAG TGGGGAAACC TCTGGACTCT TTGGTGTCAG TGGACAGCCC 
TCAGGGTTTC CTGACATTAG TGGGGAAACT TCCGGGCTTC TTGAGGTCAG TGGGCAGCCA 
TCAGGGTTTT ATGGAGAAAT ATCTGGCGTG ACTGAGCTTA GCGGACTGGC CTCTGGACAA 
CCAGAAATCA GTGGAGAAGC TTCTGGAATT CTTTCTGGCC TTGGTCCACC ATTCGGCATA 
ACCGACCTGA GCGGAGAAGC ACCCGGGATC CCTGATCTCA GTGGGCAACC ATCGGGCTTG 
CCAGAGTTCA GTGGGACAAC ATCCGGGATC CCTGACCTGG TTTCCAGTGC TGTGAGTGGC 
AGTGGTGAAT CTTCTGGCAT TACGTTCGTG GACACCAGTT TGGTTGAAGT GACCCCAACG 
ACATTTAAAG AAGAAGAAGG CTTAGGATCT GTGGAACTCA GTGGCCTCCC TTCAGGAGAG 
GTGGGTGTCT CAGGCACATC TGGGCTAGCG GATGTCAGTG GACTGTCTTC TGGAGCAATT 
GACTCCAGTG GGTTTACGTC CCAGCCTCCA GAATTCAGTG GCCTGCCAAG CGGAGTAACT 
GAGGTCAGTG GAGAAGCCTC CGGAGCTGAA AGTGGGAGCA GCCTGCCCTC CGGAGCATAT 
GACAGCAGTG GACTTCCGTC TGGTTTCCCC ACTGTCTCTT TTGTAGACAG GACTTTGGTG 
GAATCTGTAA CCCAGGCTCC AACTGCTCAA GAAGCAGGAG AAGGGCCTTC AGGCATTCTG 
GAACTTAGCG GTGCGCCTTC TGGAGCACCA GACATGTCTG GAGACCATTT GGGATCTTTG 
GACCAAAGTG GGCTTCAGTC TGGACTAGTG GAGCCCAGTG GGGAGCCCGC AAGTACTCCA 
TATTTTAGTG GGGACTTTTC TGGCACCACT GATGTAAGTG GGGAATCCTC TGCAGCCACG 
AGCACCAGTG GGGAGGCCTC CGGACTTCCA GAAGTTACGT TAATCACATC TGAGTTGGTG 
GAGGGAGTTA CTGAACCAAC CGTTTCCCAG GAACTGGGCC AGAGACCCCC CGTAACATAC 
ACCCCCCAGC TTTTTGAATC CAGTGGTGAA GCCTCTGCAT CTGGGGATGT GCCAAGGTTC 
CCTGGATCGG GGGTAGAAGT GTCATCAGTC CCAGAATCCA GCGGTGAGAC GTCAGCCTAT 
CCCGAGGCTG AGGTGGGAGC ATCTGCTGCC CCCGAGGCAA GCGGAGGAGC TTCCGGGTCC 
CCTAACCTGA GTGAAACCAC CTCCACCTTC CATGAAGCTG ATCTGGAGGG AACCTCAGGC 
CTGGGAGTGA GTGGCAGCCC CTCAGCCTTT CCAGAAGGCC CCACGGAGGG CTTGGCCACC 
CCGGAAGTGA GTGGAGAGTC AACCACTGCC TTTGACGTGA GCGTAGAGGC ATCAGGCTCA 
CCTTCTGCCA CTCCCCTGGC TTCTGGAGAC AGGACTGACA CCAGCGGAGA TCTGTCTGGC 
CACACCTCGG GGCTGGATAT TGTCATCAGC ACCACCATCC CAGAATCCGA GTGGACTCAG 
CAGACCCAGC GCCCTGCAGA GGCGCGTCTA GAAATCGAAT CCTCAAGCCC TGTGCACTCA 
GGAGAAGAGA GCCAAACAGC CGACACAGCC ACCTCCCCGA CTGATGCTTC TATCCCAGCC 
TCCGCAGGAG GGACAGATGA TTCAGAGGCA ACCACAACAG CCTCTACCAG TTCCTGTGCC 
GAAAAGCCCT GTAGCGCTGG GACCTGCCAG GAGACGGAGG GACACGTCAT ATGCTTGTGC 
CCCCCGGGCT ACCAAGGGGA CGTGTGTGAG ATTGACCAGA AGCTGTGCGA GGAGGGCTGG 
ACCAAGTTCC AGGGCCACTG TTACCGCCAC TTCCCCGACC GGGCAACCTG GGTGGACGCT 
GAGAGCCAGT GCCGGAAGCA GCAGTCACAC CTGAGCAGCA TCGTCACCCC CGAGGAGCAG 
GAGTTTGTCA ACAACAATGC CCAGGACTAC CAGTGGATCG GCCTGAACGA CAAGACCATC 
GAAGGGGACT TCCGCTGGTC AGATGGACAC TCCTTGCAAT TTGAGAACTG GCGCCCCAAC 
CAGCCTGACA ACTTCTTTGC CACTGGGGAG GACTGTGTGG TGATGATCTG GCATGAGAAG 
GGCGAGTGGA ACGATGTCCC ATGTAATTAC CAGCTACCCT TCACCTGTAA AAAGGGCACA 
GTGGCCTGCG GAGAGCCCCC CGTGGTGGAG CATGCCAGAA TCTTCGGACA GAAGAAGGAC 
CGATACGAGA TCAATGCCCT GGTGCGGTAC CAGTGCACCG AGGGCTTCAT CCAGCGCCAT 
GTGCCCACCA TCCGGTGCCA GCCCAGCGGG CACTGGGAGG AGCCTCGGAT CACCTGCACA 
GACCCCGCTA CCTACAAGCG CAGACTACAG AAGCGGAGCT CACGGCCCCT GAGGAGGAGC 
CACCCCAGCA CGGCCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837358.1
CDS	11..7111
Translation	MTTLLLVFVTLRVITAAISVEVSEPDNSLSVSIPEPSPLRVLLGSSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRISKEKEVVLLVATEGRVRVNSAYQDKVTLPNYPAIPSDATLEIQNMRSNDSGIYRCEVMHGIEDSQATLEVVVKGIVFHYRAISTRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPREGCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRLGARLATTGQLYLAWQGGMDMCSAGWLADRSVRYPISKARPNCGGNLLGVRTVYLHANQTGYPDPSSRYDAICYTGEDFVDIPESFFGVGGEEDITIQTVTWPDVELPLPRNITEGEARGSVILTAKPDFEVSPTAPEPEEPFTFVPEVRATAFPEVENRTEEATRPWAFPRESTPGLGAPTAFTSEDLVVQVTLAPGAAEVPGQPRLPGGVVFHYRPGSSRYSLTFEEAKQACLRTGAIIASPEQLQAAYEAGYEQCDAGWLQDQTVRYPIVSPRTPCVGDKDSSPGVRTYGVRPPSETYDVYCYVDRLEGEVFFATRLEQFTFWEAQEFCESQNATLATTGQLYAAWSRGLDKCYAGWLADGSLRYPIVTPRPACGGDKPGVRTVYLYPNQTGLLDPLSRHHAFCFRGVSAAPSPEEEEGSAPTAGPDVEEWMVTQVGPGVAAVPIGEETTAIPGFTVEPENKTEWELAYTPAGTLPLPGIPPTWPPTGEATEEHTEGPSATEVPSASEKPFPSEEPFPPEEPFPSEKPFPPEELFPSEKPFPSEKPFPSEEPFPSEKPFPPEELFPSEKPIPSEEPFPSEEPFPSEKPFPPEEPFPSEKPIPSEEPFPSEKPFPSEEPFPSEEPSTLSAPVPSRTELPSSGEVSGVPEISGDFTGSGEISGHLDFSGQPSGESASGLPSEDLDSSGLTSTVGSGLPVESGLPSGEEERITWTSAPKVDRLPSGGEGPEVSGVEDISGLPSGGEVHLEISASGVEDISGLPSGGEVHLEISASGVEDLSRIPSGEGPEISASGVEDISGLPSGEEGHLEISASGVEDLSGIPSGEGPEVSASGVEDLIGLPSGEGPEVSASGVEDLSRLPSGEGPEVSASGVEDLSGLPSGEGPEVSVSGVEDLSRLPSGEGPEVSASGVEDLSRLPSGEGPEISVSGVEDISILPSGEGPEVSASGVEDLSVLPSGEGHLEISTSGVEDLSVLPSGEGHLEISASGVEDISRLPSGEGPEVSASGVEDLSVLPSGEDHLEISASGVEDLGVLPSGEDHLEISASGVEDISRLPSGEGPEVSASGVEDLSVLPSGEGHLEISASGVEDLSRLPSGGEDHLETSASGVGDLSGLPSGREGLEISASGAGDLSGLTSGKEDLTGSASGALDLGRIPSVTLGSGQAPEASGLPSGFSGEYSGVDLESGPSSGLPDFSGLPSGFPTVSLVDTTLVEVVTATTAGELEGRGTIDISGAGETSGLPFSELDISGGASGLSSGAELSGQASGSPDISGETSGLFGVSGQPSGFPDISGETSGLLEVSGQPSGFYGEISGVTELSGLASGQPEISGEASGILSGLGPPFGITDLSGEAPGIPDLSGQPSGLPEFSGTTSGIPDLVSSAVSGSGESSGITFVDTSLVEVTPTTFKEEEGLGSVELSGLPSGEVGVSGTSGLADVSGLSSGAIDSSGFTSQPPEFSGLPSGVTEVSGEASGAESGSSLPSGAYDSSGLPSGFPTVSFVDRTLVESVTQAPTAQEAGEGPSGILELSGAPSGAPDMSGDHLGSLDQSGLQSGLVEPSGEPASTPYFSGDFSGTTDVSGESSAATSTSGEASGLPEVTLITSELVEGVTEPTVSQELGQRPPVTYTPQLFESSGEASASGDVPRFPGSGVEVSSVPESSGETSAYPEAEVGASAAPEASGGASGSPNLSETTSTFHEADLEGTSGLGVSGSPSAFPEGPTEGLATPEVSGESTTAFDVSVEASGSPSATPLASGDRTDTSGDLSGHTSGLDIVISTTIPESEWTQQTQRPAEARLEIESSSPVHSGEESQTADTATSPTDASIPASAGGTDDSEATTTASTSSCAEKPCSAGTCQETEGHVICLCPPGYQGDVCEIDQKLCEEGWTKFQGHCYRHFPDRATWVDAESQCRKQQSHLSSIVTPEEQEFVNNNAQDYQWIGLNDKTIEGDFRWSDGHSLQFENWRPNQPDNFFATGEDCVVMIWHEKGEWNDVPCNYQLPFTCKKGTVACGEPPVVEHARIFGQKKDRYEINALVRYQCTEGFIQRHVPTIRCQPSGHWEEPRITCTDPATYKRRLQKRSSRPLRRSHPSTAH

Target ORF information:

RefSeq Version	XM_024981590.1
Organism	Bos taurus(cattle)
Definition	Bos taurus aggrecan (ACAN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981590.1

ORF Insert Sequence:

ATGACCACTT TACTCTTGGT GTTTGTGACT CTGAGGGTCA TCACAGCAGC CATCTCAGTA 
GAAGTTTCAG AACCTGACAA CTCGCTGAGT GTCAGCATCC CTGAACCGTC CCCGCTGCGG 
GTCCTCCTGG GGAGCTCCCT CACCATCCCC TGCTACTTCA TCGACCCCAT GCACCCCGTG 
ACCACCGCCC CCTCCACCGC CCCCCTTGCC CCAAGAATCA AGTGGAGCCG CATTTCCAAG 
GAGAAGGAGG TGGTGCTGCT GGTGGCCACT GAAGGGCGGG TGCGGGTCAA CAGCGCCTAC 
CAAGACAAGG TCACGCTGCC CAACTACCCC GCCATCCCCA GCGACGCCAC CCTGGAAATC 
CAGAACATGC GCTCCAATGA CTCCGGGATC TATCGCTGCG AGGTGATGCA CGGCATCGAG 
GACAGCCAGG CCACCCTAGA GGTCGTGGTA AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCCACGCGCT ACACCCTGGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGCGCC 
ATCATCGCCA CGCCCGAGCA GCTGCAGGCT GCCTATGAGG ACGGCTTCCA CCAGTGCGAC 
GCCGGCTGGC TGGCCGATCA GACTGTGAGG TATCCCATCC ACACGCCGAG GGAAGGTTGC 
TATGGAGACA AGGACGAGTT TCCCGGCGTG AGAACCTACG GCATCCGGGA CACCAACGAA 
ACCTATGACG TGTACTGCTT CGCGGAGGAG ATGGAGGGCG AGGTCTTCTA TGCAACATCC 
CCGGAGAAGT TCACCTTCCA AGAGGCAGCC AACGAGTGCC GGCGGCTGGG CGCCCGGCTG 
GCCACCACGG GCCAACTCTA CCTGGCCTGG CAGGGTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCTG ACCGCAGCGT GCGATACCCC ATCTCCAAGG CCCGGCCTAA CTGCGGGGGC 
AACCTCCTGG GAGTGAGGAC CGTCTACCTG CACGCCAACC AGACGGGCTA CCCTGACCCT 
TCATCCCGCT ATGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAGC 
TTTTTCGGGG TGGGCGGTGA GGAGGACATC ACCATCCAGA CGGTGACCTG GCCTGACGTG 
GAGCTGCCCC TGCCCCGAAA TATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTCACG 
GCAAAGCCCG ACTTTGAAGT CTCCCCCACC GCCCCGGAAC CCGAGGAGCC TTTCACGTTT 
GTCCCTGAAG TAAGGGCCAC TGCATTCCCC GAAGTAGAGA ACAGGACTGA AGAGGCCACC 
CGGCCCTGGG CCTTTCCCAG AGAGTCCACC CCCGGCCTGG GAGCCCCCAC GGCCTTCACC 
AGCGAGGACC TCGTCGTGCA GGTGACCTTA GCCCCAGGTG CGGCTGAGGT CCCTGGGCAG 
CCACGACTGC CAGGGGGAGT CGTGTTCCAC TACCGCCCAG GCTCCTCCCG CTACTCGCTG 
ACCTTTGAGG AGGCCAAGCA GGCCTGCCTG CGCACCGGGG CCATCATCGC CTCGCCCGAG 
CAGCTCCAGG CCGCCTATGA AGCAGGCTAC GAGCAGTGTG ACGCCGGCTG GCTGCAGGAC 
CAGACAGTCA GATACCCCAT TGTGAGCCCG CGGACCCCCT GTGTGGGTGA CAAGGACAGC 
AGCCCGGGGG TCCGGACCTA CGGCGTGCGG CCACCATCAG AAACCTACGA TGTCTACTGC 
TACGTGGACA GACTCGAGGG GGAGGTGTTC TTCGCCACAC GCCTGGAGCA GTTCACCTTC 
TGGGAAGCCC AGGAGTTCTG TGAATCCCAA AACGCCACTC TGGCCACCAC AGGCCAGCTC 
TATGCTGCCT GGAGCCGTGG TCTGGACAAG TGCTACGCCG GCTGGCTGGC TGACGGCAGC 
CTCCGCTACC CCATCGTCAC CCCAAGGCCC GCCTGTGGCG GGGACAAACC GGGCGTGAGA 
ACCGTCTACC TCTACCCCAA CCAGACGGGC CTCCTGGATC CGCTGTCCCG GCACCACGCC 
TTCTGCTTCC GAGGTGTTTC AGCGGCGCCC TCTCCAGAAG AAGAGGAGGG TAGTGCACCC 
ACAGCAGGCC CTGACGTGGA GGAGTGGATG GTGACACAAG TGGGGCCTGG CGTGGCTGCT 
GTCCCCATCG GGGAGGAGAC GACTGCAATC CCAGGCTTCA CCGTTGAGCC AGAAAACAAG 
ACGGAATGGG AACTTGCCTA CACCCCAGCG GGCACTTTGC CACTACCAGG GATCCCTCCT 
ACATGGCCTC CCACTGGAGA AGCAACAGAG GAGCACACAG AAGGCCCTTC TGCAACGGAA 
GTGCCCTCAG CCTCAGAGAA GCCGTTCCCT TCAGAGGAAC CATTCCCCCC AGAGGAACCA 
TTCCCCTCAG AGAAACCATT CCCCCCAGAG GAACTGTTCC CCTCAGAGAA GCCATTCCCC 
TCAGAAAAGC CGTTCCCCTC AGAGGAACCG TTCCCCTCAG AAAAACCATT CCCCCCAGAG 
GAACTGTTCC CCTCAGAGAA GCCAATCCCC TCAGAGGAGC CATTCCCCTC AGAGGAGCCA 
TTCCCCTCAG AGAAGCCATT CCCCCCAGAG GAACCATTCC CCTCAGAGAA ACCAATCCCC 
TCAGAGGAGC CTTTCCCCTC AGAGAAGCCA TTCCCTTCAG AGGAGCCATT CCCCTCAGAG 
GAGCCATCCA CACTTTCAGC CCCAGTGCCC AGCAGGACTG AGCTGCCAAG CTCTGGGGAG 
GTGTCTGGGG TGCCCGAAAT CAGTGGTGAC TTCACAGGCA GTGGAGAAAT TTCAGGACAC 
CTAGACTTCA GTGGGCAGCC CTCAGGGGAA AGTGCAAGTG GACTGCCCTC TGAAGACCTT 
GACTCCAGTG GGCTCACCTC TACAGTGGGC TCAGGCCTGC CTGTGGAAAG TGGACTGCCT 
TCAGGGGAAG AAGAGAGAAT TACATGGACC AGTGCTCCTA AAGTTGACAG GTTGCCCTCT 
GGGGGTGAGG GCCCAGAAGT TTCTGGAGTA GAGGATATCA GTGGACTTCC TTCTGGAGGA 
GAGGTTCATC TAGAGATCTC TGCCTCTGGA GTAGAGGACA TCAGTGGACT TCCTTCTGGA 
GGAGAGGTTC ATCTAGAGAT CTCTGCCTCT GGAGTAGAGG ACCTAAGTAG AATTCCTTCT 
GGAGAAGGTC CAGAAATCTC TGCCTCTGGA GTAGAGGACA TCAGTGGACT TCCTTCTGGA 
GAGGAAGGTC ATCTAGAGAT CTCTGCCTCT GGAGTAGAAG ACCTCAGTGG AATTCCTTCT 
GGAGAAGGTC CAGAAGTCTC TGCTTCTGGA GTAGAGGACC TTATTGGACT TCCTTCTGGA 
GAAGGTCCAG AAGTCTCTGC TTCTGGAGTA GAGGACCTCA GTAGACTTCC TTCTGGAGAA 
GGTCCAGAAG TCTCTGCTTC TGGGGTAGAG GACCTTAGTG GACTTCCTTC TGGAGAAGGT 
CCAGAAGTCT CTGTTTCTGG AGTAGAGGAT CTCAGCAGAC TTCCTTCTGG AGAAGGTCCA 
GAAGTCTCTG CTTCTGGAGT AGAGGACCTC AGTAGACTTC CTTCTGGAGA AGGTCCAGAA 
ATCTCTGTGT CTGGAGTAGA GGACATCAGT ATACTTCCTT CTGGAGAAGG TCCAGAAGTC 
TCTGCCTCTG GAGTAGAGGA CCTCAGTGTT CTTCCTTCTG GAGAAGGTCA TCTAGAGATC 
TCTACCTCTG GAGTAGAGGA CCTCAGTGTT CTTCCTTCTG GTGAAGGTCA TCTAGAGATC 
TCTGCCTCTG GAGTAGAGGA CATCAGTAGA CTTCCTTCTG GAGAAGGTCC AGAAGTCTCT 
GCCTCTGGAG TAGAGGACCT CAGTGTTCTT CCTTCTGGAG AAGATCATCT AGAGATCTCT 
GCCTCTGGAG TAGAGGACCT CGGTGTTCTT CCTTCTGGAG AAGATCATCT AGAGATCTCT 
GCCTCTGGAG TAGAGGACAT CAGTAGACTT CCTTCTGGAG AAGGTCCAGA AGTCTCTGCC 
TCTGGAGTAG AGGACCTCAG TGTTCTTCCT TCTGGAGAAG GTCATCTAGA GATCTCTGCC 
TCTGGAGTAG AGGACCTCAG TAGACTTCCT TCTGGAGGAG AGGATCATCT AGAGACTTCT 
GCTTCTGGAG TAGGGGACCT TAGTGGACTT CCTTCTGGAA GGGAAGGTCT GGAGATCTCT 
GCTTCTGGAG CTGGGGATCT TAGTGGGTTG ACTTCTGGAA AAGAAGACTT GACTGGGTCA 
GCTTCTGGAG CCTTGGACCT TGGCAGAATA CCTTCTGTAA CTCTAGGAAG TGGGCAAGCT 
CCAGAAGCAA GTGGTCTTCC TTCTGGATTT AGTGGTGAGT ATTCTGGGGT GGACCTTGAA 
AGTGGCCCAT CCTCTGGCCT TCCTGATTTC AGTGGACTTC CATCTGGGTT CCCAACTGTC 
TCCCTAGTGG ATACTACATT GGTGGAAGTC GTCACAGCCA CCACAGCAGG TGAACTAGAA 
GGAAGGGGAA CCATTGACAT CAGCGGTGCT GGAGAAACAT CTGGGCTGCC CTTCAGTGAG 
TTGGACATTA GTGGAGGAGC AAGCGGACTC TCTTCAGGAG CTGAACTCAG TGGCCAAGCA 
TCTGGGTCTC CTGACATCAG TGGGGAAACC TCTGGACTCT TTGGTGTCAG TGGACAGCCC 
TCAGGGTTTC CTGACATTAG TGGGGAAACT TCCGGGCTTC TTGAGGTCAG TGGGCAGCCA 
TCAGGGTTTT ATGGAGAAAT ATCTGGCGTG ACTGAGCTTA GCGGACTGGC CTCTGGACAA 
CCAGAAATCA GTGGAGAAGC TTCTGGAATT CTTTCTGGCC TTGGTCCACC ATTCGGCATA 
ACCGACCTGA GCGGAGAAGC ACCCGGGATC CCTGATCTCA GTGGGCAACC ATCGGGCTTG 
CCAGAGTTCA GTGGGACAAC ATCCGGGATC CCTGACCTGG TTTCCAGTGC TGTGAGTGGC 
AGTGGTGAAT CTTCTGGCAT TACGTTCGTG GACACCAGTT TGGTTGAAGT GACCCCAACG 
ACATTTAAAG AAGAAGAAGG CTTAGGATCT GTGGAACTCA GTGGCCTCCC TTCAGGAGAG 
GTGGGTGTCT CAGGCACATC TGGGCTAGCG GATGTCAGTG GACTGTCTTC TGGAGCAATT 
GACTCCAGTG GGTTTACGTC CCAGCCTCCA GAATTCAGTG GCCTGCCAAG CGGAGTAACT 
GAGGTCAGTG GAGAAGCCTC CGGAGCTGAA AGTGGGAGCA GCCTGCCCTC CGGAGCATAT 
GACAGCAGTG GACTTCCGTC TGGTTTCCCC ACTGTCTCTT TTGTAGACAG GACTTTGGTG 
GAATCTGTAA CCCAGGCTCC AACTGCTCAA GAAGCAGGAG AAGGGCCTTC AGGCATTCTG 
GAACTTAGCG GTGCGCCTTC TGGAGCACCA GACATGTCTG GAGACCATTT GGGATCTTTG 
GACCAAAGTG GGCTTCAGTC TGGACTAGTG GAGCCCAGTG GGGAGCCCGC AAGTACTCCA 
TATTTTAGTG GGGACTTTTC TGGCACCACT GATGTAAGTG GGGAATCCTC TGCAGCCACG 
AGCACCAGTG GGGAGGCCTC CGGACTTCCA GAAGTTACGT TAATCACATC TGAGTTGGTG 
GAGGGAGTTA CTGAACCAAC CGTTTCCCAG GAACTGGGCC AGAGACCCCC CGTAACATAC 
ACCCCCCAGC TTTTTGAATC CAGTGGTGAA GCCTCTGCAT CTGGGGATGT GCCAAGGTTC 
CCTGGATCGG GGGTAGAAGT GTCATCAGTC CCAGAATCCA GCGGTGAGAC GTCAGCCTAT 
CCCGAGGCTG AGGTGGGAGC ATCTGCTGCC CCCGAGGCAA GCGGAGGAGC TTCCGGGTCC 
CCTAACCTGA GTGAAACCAC CTCCACCTTC CATGAAGCTG ATCTGGAGGG AACCTCAGGC 
CTGGGAGTGA GTGGCAGCCC CTCAGCCTTT CCAGAAGGCC CCACGGAGGG CTTGGCCACC 
CCGGAAGTGA GTGGAGAGTC AACCACTGCC TTTGACGTGA GCGTAGAGGC ATCAGGCTCA 
CCTTCTGCCA CTCCCCTGGC TTCTGGAGAC AGGACTGACA CCAGCGGAGA TCTGTCTGGC 
CACACCTCGG GGCTGGATAT TGTCATCAGC ACCACCATCC CAGAATCCGA GTGGACTCAG 
CAGACCCAGC GCCCTGCAGA GGCGCGTCTA GAAATCGAAT CCTCAAGCCC TGTGCACTCA 
GGAGAAGAGA GCCAAACAGC CGACACAGCC ACCTCCCCGA CTGATGCTTC TATCCCAGCC 
TCCGCAGGAG GGACAGATGA TTCAGAGGCA ACCACAACAG CCTCTACCAG TTCCTGTGCC 
GAAAAGCCCT GTAGCGCTGG GACCTGCCAG GAGACGGAGG GACACGTCAT ATGCTTGTGC 
CCCCCGGGCT ACCAAGGGGA CGTGTGTGAG ATTGACCAGA AGCTGTGCGA GGAGGGCTGG 
ACCAAGTTCC AGGGCCACTG TTACCGCCAC TTCCCCGACC GGGCAACCTG GGTGGACGCT 
GAGAGCCAGT GCCGGAAGCA GCAGTCACAC CTGAGCAGCA TCGTCACCCC CGAGGAGCAG 
GAGTTTGTCA ACAACAATGC CCAGGACTAC CAGTGGATCG GCCTGAACGA CAAGACCATC 
GAAGGGGACT TCCGCTGGTC AGATGGACAC TCCTTGCAAT TTGAGAACTG GCGCCCCAAC 
CAGCCTGACA ACTTCTTTGC CACTGGGGAG GACTGTGTGG TGATGATCTG GCATGAGAAG 
GGCGAGTGGA ACGATGTCCC ATGTAATTAC CAGCTACCCT TCACCTGTAA AAAGGGCACA 
GTGGCCTGCG GAGAGCCCCC CGTGGTGGAG CATGCCAGAA TCTTCGGACA GAAGAAGGAC 
CGATACGAGA TCAATGCCCT GGTGCGGTAC CAGTGCACCG AGGGCTTCAT CCAGCGCCAT 
GTGCCCACCA TCCGGTGCCA GCCCAGCGGG CACTGGGAGG AGCCTCGGAT CACCTGCACA 
GACCCCGCTA CCTACAAGCG CAGACTACAG AAGCGGAGCT CACGGCCCCT GAGGAGGAGC 
CACCCCAGCA CGGCCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACAN ( NM_173981.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776406.1 Bos taurus aggrecan (ACAN), mRNA.

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CloneID	OBa44496
Clone ID Related Accession (Same CDS sequence)	NM_173981.2
Accession Version	NM_173981.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	aggrecan core protein precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U76615.1. On Jun 3, 2003 this sequence version replaced NM_173981.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U76615.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145430 [ECO:0000348] ##Evidence-Data-END##

ATGACCACTT TACTCTTGGT GTTTGTGACT CTGAGGGTCA TCACAGCAGC CATCTCAGTA 
GAAGTTTCAG AACCTGACAA CTCGCTGAGT GTCAGCATCC CTGAACCGTC CCCACTTCGG 
GTCCTCCTGG GGAGCTCCCT CACCATCCCC TGCTACTTCA TCGACCCCAT GCACCCCGTG 
ACCACCGCCC CCTCCACCGC CCCCCTTGCC CCAAGAATCA AGTGGAGCCG CATTTCCAAG 
GAGAAGGAGG TGGTGCTGCT GGTGGCCACT GAAGGGCGGG TGCGGGTCAA CAGCGCCTAC 
CAAGACAAGG TCACGCTGCC CAACTACCCC GCCATCCCCA GCGACGCCAC CCTGGAAATC 
CAGAACATGC GCTCCAATGA CTCCGGGATT CTACGCTGCG AGGTGATGCA CGGCATCGAG 
GACAGCCAGG CCACCCTAGA GGTCGTGGTA AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCCACGCGCT ACACCCTGGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGCGCC 
ATCATCGCCA CGCCCGAGCA GCTGCAGGCT GCCTATGAGG ACGGCTTCCA CCAGTGCGAC 
GCCGGCTGGC TGGCCGATCA GACTGTGAGG TATCCCATCC ACACGCCGAG GGAAGGTTGC 
TATGGAGACA AGGACGAGTT TCCCGGCGTG AGAACCTACG GCATCCGGGA CACCAACGAA 
ACCTATGACG TGTACTGCTT CGCGGAGGAG ATGGAGGGCG AGGTCTTCTA TGCAACATCC 
CCGGAGAAGT TCACCTTCCA AGAGGCAGCC AACGAGTGCC GGCGGCTGGG CGCCCGGCTG 
GCCACCACGG GCCAACTCTA CCTGGCCTGG CAGGGTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCTG ACCGCAGCGT GCGATACCCC ATCTCCAAGG CCCGGCCTAA CTGCGGGGGC 
AACCTCCTGG GAGTGAGGAC CGTCTACCTG CACGCCAACC AGACGGGCTA CCCTGACCCT 
TCATCCCGCT ATGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAGC 
TTTTTCGGGG TGGGCGGTGA GGAGGACATC ACCATCCAGA CGGTGACCTG GCCTGACGTG 
GAGCTGCCCC TGCCCCGAAA TATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTCACG 
GCAAAGCCCG ACTTTGAAGT CTCCCCCACC GCCCCGGAAC CCGAGGAGCC TTTCACGTTT 
GTCCCTGAAG TAAGGGCCAC TGCATTCCCC GAAGTAGAGA ACAGGACTGA AGAGGCCACC 
CGGCCCTGGG CCTTTCCCAG AGAGTCCACC CCCGGCCTGG GAGCCCCCAC GGCCTTCACC 
AGCGAGGACC TCGTCGTGCA GGTGACCTTA GCCCCAGGTG CGGCTGAGGT CCCTGGGCAG 
CCACGACTGC CAGGGGGAGT CGTGTTCCAC TACCGCCCGG GCTCCTCCCG CTACTCGCTG 
ACCTTTGAGG AGGCCAAGCA GGCCTGCCTG CGCACCGGGG CCATCATCGC CTCGCCCGAG 
CAGCTCCAGG CCGCCTATGA AGCAGGCTAC GAGCAGTGTG ACGCCGGCTG GCTGCAGGAC 
CAGACAGTCA GATACCCCAT TGTGAGCCCG CGGACCCCCT GTGTGGGTGA CAAGGACAGC 
AGCCCGGGGG TCCGGACCTA CGGCGTGCGG CCACCATCAG AAACCTACGA TGTCTACTGC 
TACGTGGACA GACTCGAGGG GGAGGTGTTC TTCGCCACAC GCCTGGAGCA GTTCACCTTC 
TGGGAAGCCC AGGAGTTCTG TGAATCCCAA AACGCCACTC TGGCCACCAC AGGCCAGCTC 
TATGCTGCCT GGAGCCGTGG TCTGGACAAG TGCTACGCCG GCTGGCTGGC TGACGGCAGC 
CTCCGCTACC CCATCGTCAC CCCAAGGCCC GCCTGTGGCG GGGACAAACC GGGCGTGAGA 
ACCGTCTACC TCTACCCCAA CCAGACGGGC CTCCTGGATC CGCTGTCCCG GCACCACGCC 
TTCTGCTTCC GAGGTGTTTC AGCGGCGCCC TCTCCAGAAG AAGAGGAGGG TAGTGCACCC 
ACAGCAGGCC CTGACGTGGA GGAGTGGATG GTGACACAAG TGGGGCCTGG CGTGGCTGCT 
GTCCCCATCG GGGAGGAGAC GACTGCAATC CCAGGCTTCA CCGTTGAGCC AGAAAACAAG 
ACGGAATGGG AACTTGCCTA CACCCCAGCG GGCACTTTGC CACTACCAGG GATCCCTCCT 
ACATGGCCTC CCACTGGAGA AGCAACAGAG GAGCACACAG AAGGCCCTTC TGCAACGGAA 
GTGCCCTCAG CCTCAGAGAA GCCGTTCCCT TCAGAGGAAC CATTCCCCCC AGAGGAACCA 
TTCCCCTCAG AGAAACCATT CCCCCCAGAG GAACTGTTCC CCTCAGAGAA GCCATTCCCC 
TCAGAAAAGC CGTTCCCCTC AGAGGAACCG TTCCCCTCAG AAAAACCATT CCCCCCAGAG 
GAACTGTTCC CCTCAGAGAA GCCAATCCCC TCAGAGGAGC CATTCCCCTC AGAGGAGCCA 
TTCCCCTCAG AGAAGCCATT CCCCCCAGAG GAACCATTCC CCTCAGAGAA ACCAATCCCC 
TCAGAGGAGC CTTTCCCCTC AGAGAAGCCA TTCCCTTCAG AGGAGCCATT CCCCTCAGAG 
GAGCCATCCA CACTTTCAGC CCCAGTGCCC AGCAGGACTG AGCTGCCAAG CTCTGGGGAG 
GTGTCTGGGG TGCCCGAAAT CAGTGGTGAC TTCACAGGCA GTGGAGAAAT TTCAGGACAC 
CTAGACTTCA GTGGGCAGCC CTCAGGGGAA AGTGCAAGTG GACTGCCCTC TGAAGACCTT 
GACTCCAGTG GGCTCACCTC TACAGTGGGC TCAGGCCTGC CTGTGGAAAG TGGACTGCCT 
TCAGGGGAAG AAGAGAGAAT TACATGGACC AGTGCTCCTA AAGTTGACAG GTTGCCCTCT 
GGGGGTGAGG GCCCAGAAGT TTCTGGAGTA GAGGATATCA GTGGACTTCC TTCTGGAGGA 
GAGGTTCATC TAGAGATCTC TGCCTCTGGA GTAGAGGACA TCAGTGGACT TCCTTCTGGA 
GGAGAGGTTC ATCTAGAGAT CTCTGCCTCT GGAGTAGAGG ACCTAAGTAG AATTCCTTCT 
GGAGAAGGTC CAGAAATCTC TGCCTCTGGA GTAGAGGACA TCAGTGGACT TCCTTCTGGA 
GAGGAAGGTC ATCTAGAGAT CTCTGCCTCT GGAGTAGAAG ACCTCAGTGG AATTCCTTCT 
GGAGAAGGTC CAGAAGTCTC TGCTTCTGGA GTAGAGGACC TTATTGGACT TCCTTCTGGA 
GAAGGTCCAG AAGTCTCTGC TTCTGGAGTA GAGGACCTCA GTAGACTTCC TTCTGGAGAA 
GGTCCAGAAG TCTCTGCTTC TGGGGTAGAG GACCTTAGTG GACTTCCTTC TGGAGAAGGT 
CCAGAAGTCT CTGTTTCTGG AGTAGAGGAT CTCAGCAGAC TTCCTTCTGG AGAAGGTCCA 
GAAGTCTCTG CTTCTGGAGT AGAGGACCTC AGTAGACTTC CTTCTGGAGA AGGTCCAGAA 
ATCTCTGTGT CTGGAGTAGA GGACATCAGT ATACTTCCTT CTGGAGAAGG TCCAGAAGTC 
TCTGCCTCTG GAGTAGAGGA CCTCAGTGTT CTTCCTTCTG GAGAAGGTCA TCTAGAGATC 
TCTACCTCTG GAGTAGAGGA CCTCAGTGTT CTTCCTTCTG GTGAAGGTCA TCTAGAGACT 
TCCTCTGGAG TAGAGGACAT CAGTAGACTT CCTTCTGGAG AAGGTCCAGA AGTCTCTGCC 
TCTGGAGTAG AGGACCTCAG TGTTCTTCCT TCTGGAGAAG ATCATCTAGA GATCTCTGCC 
TCTGGAGTAG AGGACCTCGG TGTTCTTCCT TCTGGAGAAG ATCATCTAGA GATCTCTGCC 
TCTGGAGTAG AGGACATCAG TAGACTTCCT TCTGGAGAAG GTCCAGAAGT CTCTGCCTCT 
GGAGTAGAGG ACCTCAGTGT TCTTCCTTCT GGAGAAGGTC ATCTAGAGAT CTCTGCCTCT 
GGAGTAGAGG ACCTCAGTAG ACTTCCTTCT GGAGGAGAGG ATCATCTAGA GACTTCTGCT 
TCTGGAGTAG GGGACCTTAG TGGACTTCCT TCTGGAAGGG AAGGTCTGGA GATCTCTGCT 
TCTGGAGCTG GGGATCTTAG TGGGTTGACT TCTGGAAAAG AAGACTTGAC TGGGTCAGCT 
TCTGGAGCCT TGGACCTTGG CAGAATACCT TCTGTAACTC TAGGAAGTGG GCAAGCTCCA 
GAAGCAAGTG GTCTTCCTTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGAAAGT 
GGCCCATCCT CTGGCCTTCC TGATTTCAGT GGACTTCCAT CTGGGTTCCC AACTGTCTCC 
CTAGTGGATA CTACATTGGT GGAAGTCGTC ACAGCCACCA CAGCAGGTGA ACTAGAAGGA 
AGGGGAACCA TTGACATCAG CGGTGCTGGA GAAACATCTG GGCTGCCCTT CAGTGAGTTG 
GACATTAGTG GAGGAGCAAG CGGACTCTCT TCAGGAGCTG AACTCAGTGG CCAAGCATCT 
GGGTCTCCTG ACATCAGTGG GGAAACCTCT GGACTCTTTG GTGTCAGTGG ACAGCCCTCA 
GGGTTTCCTG ACATTAGTGG GGAAACTTCC GGGCTTCTTG AGGTCAGTGG GCAGCCATCA 
GGGTTTTATG GAGAAATATC TGGCGTGACT GAGCTTAGCG GACTGGCCTC TGGACAACCA 
GAAATCAGTG GAGAAGCTTC TGGAATTCTT TCTGGCCTTG GTCCACCATT CGGCATAACC 
GACCTGAGCG GAGAAGCACC CGGGATCCCT GATCTCAGTG GGCAACCATC GGGCTTGCCA 
GAGTTCAGTG GGACAGCATC CGGGATCCCT GACCTGGTTT CCAGTGCTGT GAGTGGCAGT 
GGTGAATCTT CTGGCATTAC GTTCGTGGAC ACCAGTTTGG TTGAAGTGAC CCCAACGACA 
TTTAAAGAAG AAGAAGGCTT AGGATCTGTG GAACTCAGTG GCCTCCCTTC AGGAGAGCTG 
GGTGTCTCAG GCACATCTGG GCTAGCGGAT GTCAGTGGAC TGTCTTCTGG AGCAATTGAC 
TCCAGTGGGT TTACGTCCCA GCCTCCAGAA TTCAGTGGCC TGCCAAGCGG AGTAACTGAG 
GTCAGTGGAG AAGCCTCCGG AGCTGAAAGT GGGAGCAGCC TGCCCTCCGG AGCATATGAC 
AGCAGTGGAC TTCCGTCTGG TTTCCCCACT GTCTCTTTTG TAGACAGGAC TTTGGTGGAA 
TCTGTAACCC AGGCTCCAAC TGCTCAAGAA GCAGGAGAAG GGCCTTCAGG CATTCTGGAA 
CTTAGCGGTG CGCCTTCTGG AGCACCAGAC ATGTCTGGAG ACCATTTGGG ATCTTTGGAC 
CAAAGTGGGC TTCAGTCTGG ACTAGTGGAG CCCAGTGGGG AGCCCGCAAG TACTCCATAT 
TTTAGTGGGG ACTTTTCTGG CACCACTGAT GTAAGTGGGG AATCCTCTGC AGCCACGAGC 
ACCAGTGGGG AGGCCTCCGG ACTTCCAGAA GTTACGTTAA TCACATCTGA GTTGGTGGAG 
GGAGTTACTG AACCAACCGT TTCCCAGGAA CTGGGCCAGA GACCCCCCGT AACATACACC 
CCCCAGCTTT TTGAATCCAG TGGTGAAGCC TCTGCATCTG GGGATGTGCC AAGGTTCCCT 
GGATCGGGGG TAGAAGTGTC ATCAGTCCCA GAATCCAGCG GTGAGACGTC AGCCTATCCC 
GAGGCTGAGG TGGGAGCATC TGCTGCCCCC GAGGCAAGCG GAGGAGCTTC CGGGTCCCCT 
AACCTGAGTG AAACCACCTC CACCTTCCAT GAAGCTGATC TGGAGGGAAC CTCAGGCCTG 
GGAGTGAGTG GCAGCCCCTC AGCCTTTCCA GAAGGCCCCA CGGAGGGCTT GGCCACCCCG 
GAAGTGAGTG GAGAGTCAAC CACTGCCTTT GACGTGAGCG TAGAGGCATC AGGCTCACCT 
TCTGCCACTC CCCTGGCTTC TGGAGACAGG ACTGACACCA GCGGAGATCT GTCTGGCCAC 
ACCTCGGGGC TGGATATTGT CATCAGCACC ACCATCCCAG AATCCGAGTG GACTCAGCAG 
ACCCAGCGCC CTGCAGAGGC GCGTCTAGAA ATCGAATCCT CAAGCCCTGT GCACTCAGGA 
GAAGAGAGCC AAACAGCCGA CACAGCCACC TCCCCGACTG ATGCTTCTAT CCCAGCCTCC 
GCAGGAGGGA CAGATGATTC AGAGGCAACC ACAACAGACC AGAAGCTGTG CGAGGAGGGC 
TGGACCAAGT TCCAGGGCCA CTGTTACCGC CACTTCCCGG ACCGGGCAAC CTGGGTGGAC 
GCTGAGAGCC AGTGCCGGAA GCAGCAGTCA CACCTGAGCA GCATCGTCAC CCCCGAGGAG 
CAGGAGTTTG TCAACAACAA TGCCCAGGAC TACCAGTGGA TCGGCCTGAA CGACAAGACC 
ATCGAAGGGG ACTTCCGCTG GTCAGATGGA CACTCCTTGC AATTTGAGAA CTGGCGCCCC 
AACCAGCCTG ACAACTTCTT TGCCACTGGG GAGGACTGTG TGGTGATGAT CTGGCATGAG 
AAGGGCGAGT GGAACGATGT CCCATGTAAT TACCAGCTAC CCTTCACCTG TAAAAAGGGC 
ACAGTGGCCT GCGGAGAGCC CCCCGTGGTG GAGCATGCCA GAATCTTCGG ACAGAAGAAG 
GACCGATACG AGATCAATGC CCTGGTGCGG TACCAGTGCA CCGAGGGCTT CATCCAGGGC 
CATGTGCCCA CCATCCGGTG CCAGCCCAGC GGGCACTGGG AGGAGCCTCG GATCACCTGC 
ACAGACCCCG CTACCTACAA GCGCAGACTA CAGAAGCGGA GCTCACGGCC CCTGAGGAGG 
AGCCACCCCA GCACGGCCCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776406.1
CDS	361..7344
Translation	MTTLLLVFVTLRVITAAISVEVSEPDNSLSVSIPEPSPLRVLLGSSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRISKEKEVVLLVATEGRVRVNSAYQDKVTLPNYPAIPSDATLEIQNMRSNDSGILRCEVMHGIEDSQATLEVVVKGIVFHYRAISTRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPREGCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRLGARLATTGQLYLAWQGGMDMCSAGWLADRSVRYPISKARPNCGGNLLGVRTVYLHANQTGYPDPSSRYDAICYTGEDFVDIPESFFGVGGEEDITIQTVTWPDVELPLPRNITEGEARGSVILTAKPDFEVSPTAPEPEEPFTFVPEVRATAFPEVENRTEEATRPWAFPRESTPGLGAPTAFTSEDLVVQVTLAPGAAEVPGQPRLPGGVVFHYRPGSSRYSLTFEEAKQACLRTGAIIASPEQLQAAYEAGYEQCDAGWLQDQTVRYPIVSPRTPCVGDKDSSPGVRTYGVRPPSETYDVYCYVDRLEGEVFFATRLEQFTFWEAQEFCESQNATLATTGQLYAAWSRGLDKCYAGWLADGSLRYPIVTPRPACGGDKPGVRTVYLYPNQTGLLDPLSRHHAFCFRGVSAAPSPEEEEGSAPTAGPDVEEWMVTQVGPGVAAVPIGEETTAIPGFTVEPENKTEWELAYTPAGTLPLPGIPPTWPPTGEATEEHTEGPSATEVPSASEKPFPSEEPFPPEEPFPSEKPFPPEELFPSEKPFPSEKPFPSEEPFPSEKPFPPEELFPSEKPIPSEEPFPSEEPFPSEKPFPPEEPFPSEKPIPSEEPFPSEKPFPSEEPFPSEEPSTLSAPVPSRTELPSSGEVSGVPEISGDFTGSGEISGHLDFSGQPSGESASGLPSEDLDSSGLTSTVGSGLPVESGLPSGEEERITWTSAPKVDRLPSGGEGPEVSGVEDISGLPSGGEVHLEISASGVEDISGLPSGGEVHLEISASGVEDLSRIPSGEGPEISASGVEDISGLPSGEEGHLEISASGVEDLSGIPSGEGPEVSASGVEDLIGLPSGEGPEVSASGVEDLSRLPSGEGPEVSASGVEDLSGLPSGEGPEVSVSGVEDLSRLPSGEGPEVSASGVEDLSRLPSGEGPEISVSGVEDISILPSGEGPEVSASGVEDLSVLPSGEGHLEISTSGVEDLSVLPSGEGHLETSSGVEDISRLPSGEGPEVSASGVEDLSVLPSGEDHLEISASGVEDLGVLPSGEDHLEISASGVEDISRLPSGEGPEVSASGVEDLSVLPSGEGHLEISASGVEDLSRLPSGGEDHLETSASGVGDLSGLPSGREGLEISASGAGDLSGLTSGKEDLTGSASGALDLGRIPSVTLGSGQAPEASGLPSGFSGEYSGVDLESGPSSGLPDFSGLPSGFPTVSLVDTTLVEVVTATTAGELEGRGTIDISGAGETSGLPFSELDISGGASGLSSGAELSGQASGSPDISGETSGLFGVSGQPSGFPDISGETSGLLEVSGQPSGFYGEISGVTELSGLASGQPEISGEASGILSGLGPPFGITDLSGEAPGIPDLSGQPSGLPEFSGTASGIPDLVSSAVSGSGESSGITFVDTSLVEVTPTTFKEEEGLGSVELSGLPSGELGVSGTSGLADVSGLSSGAIDSSGFTSQPPEFSGLPSGVTEVSGEASGAESGSSLPSGAYDSSGLPSGFPTVSFVDRTLVESVTQAPTAQEAGEGPSGILELSGAPSGAPDMSGDHLGSLDQSGLQSGLVEPSGEPASTPYFSGDFSGTTDVSGESSAATSTSGEASGLPEVTLITSELVEGVTEPTVSQELGQRPPVTYTPQLFESSGEASASGDVPRFPGSGVEVSSVPESSGETSAYPEAEVGASAAPEASGGASGSPNLSETTSTFHEADLEGTSGLGVSGSPSAFPEGPTEGLATPEVSGESTTAFDVSVEASGSPSATPLASGDRTDTSGDLSGHTSGLDIVISTTIPESEWTQQTQRPAEARLEIESSSPVHSGEESQTADTATSPTDASIPASAGGTDDSEATTTDQKLCEEGWTKFQGHCYRHFPDRATWVDAESQCRKQQSHLSSIVTPEEQEFVNNNAQDYQWIGLNDKTIEGDFRWSDGHSLQFENWRPNQPDNFFATGEDCVVMIWHEKGEWNDVPCNYQLPFTCKKGTVACGEPPVVEHARIFGQKKDRYEINALVRYQCTEGFIQGHVPTIRCQPSGHWEEPRITCTDPATYKRRLQKRSSRPLRRSHPSTAH

Target ORF information:

RefSeq Version	NM_173981.2
Organism	Bos taurus(cattle)
Definition	Bos taurus aggrecan (ACAN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173981.2

ORF Insert Sequence:

ATGACCACTT TACTCTTGGT GTTTGTGACT CTGAGGGTCA TCACAGCAGC CATCTCAGTA 
GAAGTTTCAG AACCTGACAA CTCGCTGAGT GTCAGCATCC CTGAACCGTC CCCACTTCGG 
GTCCTCCTGG GGAGCTCCCT CACCATCCCC TGCTACTTCA TCGACCCCAT GCACCCCGTG 
ACCACCGCCC CCTCCACCGC CCCCCTTGCC CCAAGAATCA AGTGGAGCCG CATTTCCAAG 
GAGAAGGAGG TGGTGCTGCT GGTGGCCACT GAAGGGCGGG TGCGGGTCAA CAGCGCCTAC 
CAAGACAAGG TCACGCTGCC CAACTACCCC GCCATCCCCA GCGACGCCAC CCTGGAAATC 
CAGAACATGC GCTCCAATGA CTCCGGGATT CTACGCTGCG AGGTGATGCA CGGCATCGAG 
GACAGCCAGG CCACCCTAGA GGTCGTGGTA AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCCACGCGCT ACACCCTGGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGCGCC 
ATCATCGCCA CGCCCGAGCA GCTGCAGGCT GCCTATGAGG ACGGCTTCCA CCAGTGCGAC 
GCCGGCTGGC TGGCCGATCA GACTGTGAGG TATCCCATCC ACACGCCGAG GGAAGGTTGC 
TATGGAGACA AGGACGAGTT TCCCGGCGTG AGAACCTACG GCATCCGGGA CACCAACGAA 
ACCTATGACG TGTACTGCTT CGCGGAGGAG ATGGAGGGCG AGGTCTTCTA TGCAACATCC 
CCGGAGAAGT TCACCTTCCA AGAGGCAGCC AACGAGTGCC GGCGGCTGGG CGCCCGGCTG 
GCCACCACGG GCCAACTCTA CCTGGCCTGG CAGGGTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCTG ACCGCAGCGT GCGATACCCC ATCTCCAAGG CCCGGCCTAA CTGCGGGGGC 
AACCTCCTGG GAGTGAGGAC CGTCTACCTG CACGCCAACC AGACGGGCTA CCCTGACCCT 
TCATCCCGCT ATGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAGC 
TTTTTCGGGG TGGGCGGTGA GGAGGACATC ACCATCCAGA CGGTGACCTG GCCTGACGTG 
GAGCTGCCCC TGCCCCGAAA TATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTCACG 
GCAAAGCCCG ACTTTGAAGT CTCCCCCACC GCCCCGGAAC CCGAGGAGCC TTTCACGTTT 
GTCCCTGAAG TAAGGGCCAC TGCATTCCCC GAAGTAGAGA ACAGGACTGA AGAGGCCACC 
CGGCCCTGGG CCTTTCCCAG AGAGTCCACC CCCGGCCTGG GAGCCCCCAC GGCCTTCACC 
AGCGAGGACC TCGTCGTGCA GGTGACCTTA GCCCCAGGTG CGGCTGAGGT CCCTGGGCAG 
CCACGACTGC CAGGGGGAGT CGTGTTCCAC TACCGCCCGG GCTCCTCCCG CTACTCGCTG 
ACCTTTGAGG AGGCCAAGCA GGCCTGCCTG CGCACCGGGG CCATCATCGC CTCGCCCGAG 
CAGCTCCAGG CCGCCTATGA AGCAGGCTAC GAGCAGTGTG ACGCCGGCTG GCTGCAGGAC 
CAGACAGTCA GATACCCCAT TGTGAGCCCG CGGACCCCCT GTGTGGGTGA CAAGGACAGC 
AGCCCGGGGG TCCGGACCTA CGGCGTGCGG CCACCATCAG AAACCTACGA TGTCTACTGC 
TACGTGGACA GACTCGAGGG GGAGGTGTTC TTCGCCACAC GCCTGGAGCA GTTCACCTTC 
TGGGAAGCCC AGGAGTTCTG TGAATCCCAA AACGCCACTC TGGCCACCAC AGGCCAGCTC 
TATGCTGCCT GGAGCCGTGG TCTGGACAAG TGCTACGCCG GCTGGCTGGC TGACGGCAGC 
CTCCGCTACC CCATCGTCAC CCCAAGGCCC GCCTGTGGCG GGGACAAACC GGGCGTGAGA 
ACCGTCTACC TCTACCCCAA CCAGACGGGC CTCCTGGATC CGCTGTCCCG GCACCACGCC 
TTCTGCTTCC GAGGTGTTTC AGCGGCGCCC TCTCCAGAAG AAGAGGAGGG TAGTGCACCC 
ACAGCAGGCC CTGACGTGGA GGAGTGGATG GTGACACAAG TGGGGCCTGG CGTGGCTGCT 
GTCCCCATCG GGGAGGAGAC GACTGCAATC CCAGGCTTCA CCGTTGAGCC AGAAAACAAG 
ACGGAATGGG AACTTGCCTA CACCCCAGCG GGCACTTTGC CACTACCAGG GATCCCTCCT 
ACATGGCCTC CCACTGGAGA AGCAACAGAG GAGCACACAG AAGGCCCTTC TGCAACGGAA 
GTGCCCTCAG CCTCAGAGAA GCCGTTCCCT TCAGAGGAAC CATTCCCCCC AGAGGAACCA 
TTCCCCTCAG AGAAACCATT CCCCCCAGAG GAACTGTTCC CCTCAGAGAA GCCATTCCCC 
TCAGAAAAGC CGTTCCCCTC AGAGGAACCG TTCCCCTCAG AAAAACCATT CCCCCCAGAG 
GAACTGTTCC CCTCAGAGAA GCCAATCCCC TCAGAGGAGC CATTCCCCTC AGAGGAGCCA 
TTCCCCTCAG AGAAGCCATT CCCCCCAGAG GAACCATTCC CCTCAGAGAA ACCAATCCCC 
TCAGAGGAGC CTTTCCCCTC AGAGAAGCCA TTCCCTTCAG AGGAGCCATT CCCCTCAGAG 
GAGCCATCCA CACTTTCAGC CCCAGTGCCC AGCAGGACTG AGCTGCCAAG CTCTGGGGAG 
GTGTCTGGGG TGCCCGAAAT CAGTGGTGAC TTCACAGGCA GTGGAGAAAT TTCAGGACAC 
CTAGACTTCA GTGGGCAGCC CTCAGGGGAA AGTGCAAGTG GACTGCCCTC TGAAGACCTT 
GACTCCAGTG GGCTCACCTC TACAGTGGGC TCAGGCCTGC CTGTGGAAAG TGGACTGCCT 
TCAGGGGAAG AAGAGAGAAT TACATGGACC AGTGCTCCTA AAGTTGACAG GTTGCCCTCT 
GGGGGTGAGG GCCCAGAAGT TTCTGGAGTA GAGGATATCA GTGGACTTCC TTCTGGAGGA 
GAGGTTCATC TAGAGATCTC TGCCTCTGGA GTAGAGGACA TCAGTGGACT TCCTTCTGGA 
GGAGAGGTTC ATCTAGAGAT CTCTGCCTCT GGAGTAGAGG ACCTAAGTAG AATTCCTTCT 
GGAGAAGGTC CAGAAATCTC TGCCTCTGGA GTAGAGGACA TCAGTGGACT TCCTTCTGGA 
GAGGAAGGTC ATCTAGAGAT CTCTGCCTCT GGAGTAGAAG ACCTCAGTGG AATTCCTTCT 
GGAGAAGGTC CAGAAGTCTC TGCTTCTGGA GTAGAGGACC TTATTGGACT TCCTTCTGGA 
GAAGGTCCAG AAGTCTCTGC TTCTGGAGTA GAGGACCTCA GTAGACTTCC TTCTGGAGAA 
GGTCCAGAAG TCTCTGCTTC TGGGGTAGAG GACCTTAGTG GACTTCCTTC TGGAGAAGGT 
CCAGAAGTCT CTGTTTCTGG AGTAGAGGAT CTCAGCAGAC TTCCTTCTGG AGAAGGTCCA 
GAAGTCTCTG CTTCTGGAGT AGAGGACCTC AGTAGACTTC CTTCTGGAGA AGGTCCAGAA 
ATCTCTGTGT CTGGAGTAGA GGACATCAGT ATACTTCCTT CTGGAGAAGG TCCAGAAGTC 
TCTGCCTCTG GAGTAGAGGA CCTCAGTGTT CTTCCTTCTG GAGAAGGTCA TCTAGAGATC 
TCTACCTCTG GAGTAGAGGA CCTCAGTGTT CTTCCTTCTG GTGAAGGTCA TCTAGAGACT 
TCCTCTGGAG TAGAGGACAT CAGTAGACTT CCTTCTGGAG AAGGTCCAGA AGTCTCTGCC 
TCTGGAGTAG AGGACCTCAG TGTTCTTCCT TCTGGAGAAG ATCATCTAGA GATCTCTGCC 
TCTGGAGTAG AGGACCTCGG TGTTCTTCCT TCTGGAGAAG ATCATCTAGA GATCTCTGCC 
TCTGGAGTAG AGGACATCAG TAGACTTCCT TCTGGAGAAG GTCCAGAAGT CTCTGCCTCT 
GGAGTAGAGG ACCTCAGTGT TCTTCCTTCT GGAGAAGGTC ATCTAGAGAT CTCTGCCTCT 
GGAGTAGAGG ACCTCAGTAG ACTTCCTTCT GGAGGAGAGG ATCATCTAGA GACTTCTGCT 
TCTGGAGTAG GGGACCTTAG TGGACTTCCT TCTGGAAGGG AAGGTCTGGA GATCTCTGCT 
TCTGGAGCTG GGGATCTTAG TGGGTTGACT TCTGGAAAAG AAGACTTGAC TGGGTCAGCT 
TCTGGAGCCT TGGACCTTGG CAGAATACCT TCTGTAACTC TAGGAAGTGG GCAAGCTCCA 
GAAGCAAGTG GTCTTCCTTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGAAAGT 
GGCCCATCCT CTGGCCTTCC TGATTTCAGT GGACTTCCAT CTGGGTTCCC AACTGTCTCC 
CTAGTGGATA CTACATTGGT GGAAGTCGTC ACAGCCACCA CAGCAGGTGA ACTAGAAGGA 
AGGGGAACCA TTGACATCAG CGGTGCTGGA GAAACATCTG GGCTGCCCTT CAGTGAGTTG 
GACATTAGTG GAGGAGCAAG CGGACTCTCT TCAGGAGCTG AACTCAGTGG CCAAGCATCT 
GGGTCTCCTG ACATCAGTGG GGAAACCTCT GGACTCTTTG GTGTCAGTGG ACAGCCCTCA 
GGGTTTCCTG ACATTAGTGG GGAAACTTCC GGGCTTCTTG AGGTCAGTGG GCAGCCATCA 
GGGTTTTATG GAGAAATATC TGGCGTGACT GAGCTTAGCG GACTGGCCTC TGGACAACCA 
GAAATCAGTG GAGAAGCTTC TGGAATTCTT TCTGGCCTTG GTCCACCATT CGGCATAACC 
GACCTGAGCG GAGAAGCACC CGGGATCCCT GATCTCAGTG GGCAACCATC GGGCTTGCCA 
GAGTTCAGTG GGACAGCATC CGGGATCCCT GACCTGGTTT CCAGTGCTGT GAGTGGCAGT 
GGTGAATCTT CTGGCATTAC GTTCGTGGAC ACCAGTTTGG TTGAAGTGAC CCCAACGACA 
TTTAAAGAAG AAGAAGGCTT AGGATCTGTG GAACTCAGTG GCCTCCCTTC AGGAGAGCTG 
GGTGTCTCAG GCACATCTGG GCTAGCGGAT GTCAGTGGAC TGTCTTCTGG AGCAATTGAC 
TCCAGTGGGT TTACGTCCCA GCCTCCAGAA TTCAGTGGCC TGCCAAGCGG AGTAACTGAG 
GTCAGTGGAG AAGCCTCCGG AGCTGAAAGT GGGAGCAGCC TGCCCTCCGG AGCATATGAC 
AGCAGTGGAC TTCCGTCTGG TTTCCCCACT GTCTCTTTTG TAGACAGGAC TTTGGTGGAA 
TCTGTAACCC AGGCTCCAAC TGCTCAAGAA GCAGGAGAAG GGCCTTCAGG CATTCTGGAA 
CTTAGCGGTG CGCCTTCTGG AGCACCAGAC ATGTCTGGAG ACCATTTGGG ATCTTTGGAC 
CAAAGTGGGC TTCAGTCTGG ACTAGTGGAG CCCAGTGGGG AGCCCGCAAG TACTCCATAT 
TTTAGTGGGG ACTTTTCTGG CACCACTGAT GTAAGTGGGG AATCCTCTGC AGCCACGAGC 
ACCAGTGGGG AGGCCTCCGG ACTTCCAGAA GTTACGTTAA TCACATCTGA GTTGGTGGAG 
GGAGTTACTG AACCAACCGT TTCCCAGGAA CTGGGCCAGA GACCCCCCGT AACATACACC 
CCCCAGCTTT TTGAATCCAG TGGTGAAGCC TCTGCATCTG GGGATGTGCC AAGGTTCCCT 
GGATCGGGGG TAGAAGTGTC ATCAGTCCCA GAATCCAGCG GTGAGACGTC AGCCTATCCC 
GAGGCTGAGG TGGGAGCATC TGCTGCCCCC GAGGCAAGCG GAGGAGCTTC CGGGTCCCCT 
AACCTGAGTG AAACCACCTC CACCTTCCAT GAAGCTGATC TGGAGGGAAC CTCAGGCCTG 
GGAGTGAGTG GCAGCCCCTC AGCCTTTCCA GAAGGCCCCA CGGAGGGCTT GGCCACCCCG 
GAAGTGAGTG GAGAGTCAAC CACTGCCTTT GACGTGAGCG TAGAGGCATC AGGCTCACCT 
TCTGCCACTC CCCTGGCTTC TGGAGACAGG ACTGACACCA GCGGAGATCT GTCTGGCCAC 
ACCTCGGGGC TGGATATTGT CATCAGCACC ACCATCCCAG AATCCGAGTG GACTCAGCAG 
ACCCAGCGCC CTGCAGAGGC GCGTCTAGAA ATCGAATCCT CAAGCCCTGT GCACTCAGGA 
GAAGAGAGCC AAACAGCCGA CACAGCCACC TCCCCGACTG ATGCTTCTAT CCCAGCCTCC 
GCAGGAGGGA CAGATGATTC AGAGGCAACC ACAACAGACC AGAAGCTGTG CGAGGAGGGC 
TGGACCAAGT TCCAGGGCCA CTGTTACCGC CACTTCCCGG ACCGGGCAAC CTGGGTGGAC 
GCTGAGAGCC AGTGCCGGAA GCAGCAGTCA CACCTGAGCA GCATCGTCAC CCCCGAGGAG 
CAGGAGTTTG TCAACAACAA TGCCCAGGAC TACCAGTGGA TCGGCCTGAA CGACAAGACC 
ATCGAAGGGG ACTTCCGCTG GTCAGATGGA CACTCCTTGC AATTTGAGAA CTGGCGCCCC 
AACCAGCCTG ACAACTTCTT TGCCACTGGG GAGGACTGTG TGGTGATGAT CTGGCATGAG 
AAGGGCGAGT GGAACGATGT CCCATGTAAT TACCAGCTAC CCTTCACCTG TAAAAAGGGC 
ACAGTGGCCT GCGGAGAGCC CCCCGTGGTG GAGCATGCCA GAATCTTCGG ACAGAAGAAG 
GACCGATACG AGATCAATGC CCTGGTGCGG TACCAGTGCA CCGAGGGCTT CATCCAGGGC 
CATGTGCCCA CCATCCGGTG CCAGCCCAGC GGGCACTGGG AGGAGCCTCG GATCACCTGC 
ACAGACCCCG CTACCTACAA GCGCAGACTA CAGAAGCGGA GCTCACGGCC CCTGAGGAGG 
AGCCACCCCA GCACGGCCCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACAN ( XM_024981588.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837356.1 Bos taurus aggrecan (ACAN), transcript variant X1, mRNA.

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CloneID	OBa30830
Clone ID Related Accession (Same CDS sequence)	XM_024981588.1 , XM_005221803.3
Accession Version	XM_024981588.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	aggrecan core protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTT TACTCTTGGT GTTTGTGACT CTGAGGGTCA TCACAGCAGC CATCTCAGTA 
GAAGTTTCAG AACCTGACAA CTCGCTGAGT GTCAGCATCC CTGAACCGTC CCCGCTGCGG 
GTCCTCCTGG GGAGCTCCCT CACCATCCCC TGCTACTTCA TCGACCCCAT GCACCCCGTG 
ACCACCGCCC CCTCCACCGC CCCCCTTGCC CCAAGAATCA AGTGGAGCCG CATTTCCAAG 
GAGAAGGAGG TGGTGCTGCT GGTGGCCACT GAAGGGCGGG TGCGGGTCAA CAGCGCCTAC 
CAAGACAAGG TCACGCTGCC CAACTACCCC GCCATCCCCA GCGACGCCAC CCTGGAAATC 
CAGAACATGC GCTCCAATGA CTCCGGGATC TATCGCTGCG AGGTGATGCA CGGCATCGAG 
GACAGCCAGG CCACCCTAGA GGTCGTGGTA AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCCACGCGCT ACACCCTGGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGCGCC 
ATCATCGCCA CGCCCGAGCA GCTGCAGGCT GCCTATGAGG ACGGCTTCCA CCAGTGCGAC 
GCCGGCTGGC TGGCCGATCA GACTGTGAGG TATCCCATCC ACACGCCGAG GGAAGGTTGC 
TATGGAGACA AGGACGAGTT TCCCGGCGTG AGAACCTACG GCATCCGGGA CACCAACGAA 
ACCTATGACG TGTACTGCTT CGCGGAGGAG ATGGAGGGCG AGGTCTTCTA TGCAACATCC 
CCGGAGAAGT TCACCTTCCA AGAGGCAGCC AACGAGTGCC GGCGGCTGGG CGCCCGGCTG 
GCCACCACGG GCCAACTCTA CCTGGCCTGG CAGGGTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCTG ACCGCAGCGT GCGATACCCC ATCTCCAAGG CCCGGCCTAA CTGCGGGGGC 
AACCTCCTGG GAGTGAGGAC CGTCTACCTG CACGCCAACC AGACGGGCTA CCCTGACCCT 
TCATCCCGCT ATGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAGC 
TTTTTCGGGG TGGGCGGTGA GGAGGACATC ACCATCCAGA CGGTGACCTG GCCTGACGTG 
GAGCTGCCCC TGCCCCGAAA TATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTCACG 
GCAAAGCCCG ACTTTGAAGT CTCCCCCACC GCCCCGGAAC CCGAGGAGCC TTTCACGTTT 
GTCCCTGAAG TAAGGGCCAC TGCATTCCCC GAAGTAGAGA ACAGGACTGA AGAGGCCACC 
CGGCCCTGGG CCTTTCCCAG AGAGTCCACC CCCGGCCTGG GAGCCCCCAC GGCCTTCACC 
AGCGAGGACC TCGTCGTGCA GGTGACCTTA GCCCCAGGTG CGGCTGAGGT CCCTGGGCAG 
CCACGACTGC CAGGGGGAGT CGTGTTCCAC TACCGCCCAG GCTCCTCCCG CTACTCGCTG 
ACCTTTGAGG AGGCCAAGCA GGCCTGCCTG CGCACCGGGG CCATCATCGC CTCGCCCGAG 
CAGCTCCAGG CCGCCTATGA AGCAGGCTAC GAGCAGTGTG ACGCCGGCTG GCTGCAGGAC 
CAGACAGTCA GATACCCCAT TGTGAGCCCG CGGACCCCCT GTGTGGGTGA CAAGGACAGC 
AGCCCGGGGG TCCGGACCTA CGGCGTGCGG CCACCATCAG AAACCTACGA TGTCTACTGC 
TACGTGGACA GACTCGAGGG GGAGGTGTTC TTCGCCACAC GCCTGGAGCA GTTCACCTTC 
TGGGAAGCCC AGGAGTTCTG TGAATCCCAA AACGCCACTC TGGCCACCAC AGGCCAGCTC 
TATGCTGCCT GGAGCCGTGG TCTGGACAAG TGCTACGCCG GCTGGCTGGC TGACGGCAGC 
CTCCGCTACC CCATCGTCAC CCCAAGGCCC GCCTGTGGCG GGGACAAACC GGGCGTGAGA 
ACCGTCTACC TCTACCCCAA CCAGACGGGC CTCCTGGATC CGCTGTCCCG GCACCACGCC 
TTCTGCTTCC GAGGTGTTTC AGCGGCGCCC TCTCCAGAAG AAGAGGAGGG TAGTGCACCC 
ACAGCAGGCC CTGACGTGGA GGAGTGGATG GTGACACAAG TGGGGCCTGG CGTGGCTGCT 
GTCCCCATCG GGGAGGAGAC GACTGCAATC CCAGGCTTCA CCGTTGAGCC AGAAAACAAG 
ACGGAATGGG AACTTGCCTA CACCCCAGCG GGCACTTTGC CACTACCAGG GATCCCTCCT 
ACATGGCCTC CCACTGGAGA AGCAACAGAG GAGCACACAG AAGGCCCTTC TGCAACGGAA 
GTGCCCTCAG CCTCAGAGAA GCCGTTCCCT TCAGAGGAAC CATTCCCCCC AGAGGAACCA 
TTCCCCTCAG AGAAACCATT CCCCCCAGAG GAACTGTTCC CCTCAGAGAA GCCATTCCCC 
TCAGAAAAGC CGTTCCCCTC AGAGGAACCG TTCCCCTCAG AAAAACCATT CCCCCCAGAG 
GAACTGTTCC CCTCAGAGAA GCCAATCCCC TCAGAGGAGC CATTCCCCTC AGAGGAGCCA 
TTCCCCTCAG AGAAGCCATT CCCCCCAGAG GAACCATTCC CCTCAGAGAA ACCAATCCCC 
TCAGAGGAGC CTTTCCCCTC AGAGAAGCCA TTCCCTTCAG AGGAGCCATT CCCCTCAGAG 
GAGCCATCCA CACTTTCAGC CCCAGTGCCC AGCAGGACTG AGCTGCCAAG CTCTGGGGAG 
GTGTCTGGGG TGCCCGAAAT CAGTGGTGAC TTCACAGGCA GTGGAGAAAT TTCAGGACAC 
CTAGACTTCA GTGGGCAGCC CTCAGGGGAA AGTGCAAGTG GACTGCCCTC TGAAGACCTT 
GACTCCAGTG GGCTCACCTC TACAGTGGGC TCAGGCCTGC CTGTGGAAAG TGGACTGCCT 
TCAGGGGAAG AAGAGAGAAT TACATGGACC AGTGCTCCTA AAGTTGACAG GTTGCCCTCT 
GGGGGTGAGG GCCCAGAAGT TTCTGGAGTA GAGGATATCA GTGGACTTCC TTCTGGAGGA 
GAGGTTCATC TAGAGATCTC TGCCTCTGGA GTAGAGGACA TCAGTGGACT TCCTTCTGGA 
GGAGAGGTTC ATCTAGAGAT CTCTGCCTCT GGAGTAGAGG ACCTAAGTAG AATTCCTTCT 
GGAGAAGGTC CAGAAATCTC TGCCTCTGGA GTAGAGGACA TCAGTGGACT TCCTTCTGGA 
GAGGAAGGTC ATCTAGAGAT CTCTGCCTCT GGAGTAGAAG ACCTCAGTGG AATTCCTTCT 
GGAGAAGGTC CAGAAGTCTC TGCTTCTGGA GTAGAGGACC TTATTGGACT TCCTTCTGGA 
GAAGGTCCAG AAGTCTCTGC TTCTGGAGTA GAGGACCTCA GTAGACTTCC TTCTGGAGAA 
GGTCCAGAAG TCTCTGCTTC TGGGGTAGAG GACCTTAGTG GACTTCCTTC TGGAGAAGGT 
CCAGAAGTCT CTGTTTCTGG AGTAGAGGAT CTCAGCAGAC TTCCTTCTGG AGAAGGTCCA 
GAAGTCTCTG CTTCTGGAGT AGAGGACCTC AGTAGACTTC CTTCTGGAGA AGGTCCAGAA 
ATCTCTGTGT CTGGAGTAGA GGACATCAGT ATACTTCCTT CTGGAGAAGG TCCAGAAGTC 
TCTGCCTCTG GAGTAGAGGA CCTCAGTGTT CTTCCTTCTG GAGAAGGTCA TCTAGAGATC 
TCTACCTCTG GAGTAGAGGA CCTCAGTGTT CTTCCTTCTG GTGAAGGTCA TCTAGAGATC 
TCTGCCTCTG GAGTAGAGGA CATCAGTAGA CTTCCTTCTG GAGAAGGTCC AGAAGTCTCT 
GCCTCTGGAG TAGAGGACCT CAGTGTTCTT CCTTCTGGAG AAGATCATCT AGAGATCTCT 
GCCTCTGGAG TAGAGGACCT CGGTGTTCTT CCTTCTGGAG AAGATCATCT AGAGATCTCT 
GCCTCTGGAG TAGAGGACAT CAGTAGACTT CCTTCTGGAG AAGGTCCAGA AGTCTCTGCC 
TCTGGAGTAG AGGACCTCAG TGTTCTTCCT TCTGGAGAAG GTCATCTAGA GATCTCTGCC 
TCTGGAGTAG AGGACCTCAG TAGACTTCCT TCTGGAGGAG AGGATCATCT AGAGACTTCT 
GCTTCTGGAG TAGGGGACCT TAGTGGACTT CCTTCTGGAA GGGAAGGTCT GGAGATCTCT 
GCTTCTGGAG CTGGGGATCT TAGTGGGTTG ACTTCTGGAA AAGAAGACTT GACTGGGTCA 
GCTTCTGGAG CCTTGGACCT TGGCAGAATA CCTTCTGTAA CTCTAGGAAG TGGGCAAGCT 
CCAGAAGCAA GTGGTCTTCC TTCTGGATTT AGTGGTGAGT ATTCTGGGGT GGACCTTGAA 
AGTGGCCCAT CCTCTGGCCT TCCTGATTTC AGTGGACTTC CATCTGGGTT CCCAACTGTC 
TCCCTAGTGG ATACTACATT GGTGGAAGTC GTCACAGCCA CCACAGCAGG TGAACTAGAA 
GGAAGGGGAA CCATTGACAT CAGCGGTGCT GGAGAAACAT CTGGGCTGCC CTTCAGTGAG 
TTGGACATTA GTGGAGGAGC AAGCGGACTC TCTTCAGGAG CTGAACTCAG TGGCCAAGCA 
TCTGGGTCTC CTGACATCAG TGGGGAAACC TCTGGACTCT TTGGTGTCAG TGGACAGCCC 
TCAGGGTTTC CTGACATTAG TGGGGAAACT TCCGGGCTTC TTGAGGTCAG TGGGCAGCCA 
TCAGGGTTTT ATGGAGAAAT ATCTGGCGTG ACTGAGCTTA GCGGACTGGC CTCTGGACAA 
CCAGAAATCA GTGGAGAAGC TTCTGGAATT CTTTCTGGCC TTGGTCCACC ATTCGGCATA 
ACCGACCTGA GCGGAGAAGC ACCCGGGATC CCTGATCTCA GTGGGCAACC ATCGGGCTTG 
CCAGAGTTCA GTGGGACAAC ATCCGGGATC CCTGACCTGG TTTCCAGTGC TGTGAGTGGC 
AGTGGTGAAT CTTCTGGCAT TACGTTCGTG GACACCAGTT TGGTTGAAGT GACCCCAACG 
ACATTTAAAG AAGAAGAAGG CTTAGGATCT GTGGAACTCA GTGGCCTCCC TTCAGGAGAG 
GTGGGTGTCT CAGGCACATC TGGGCTAGCG GATGTCAGTG GACTGTCTTC TGGAGCAATT 
GACTCCAGTG GGTTTACGTC CCAGCCTCCA GAATTCAGTG GCCTGCCAAG CGGAGTAACT 
GAGGTCAGTG GAGAAGCCTC CGGAGCTGAA AGTGGGAGCA GCCTGCCCTC CGGAGCATAT 
GACAGCAGTG GACTTCCGTC TGGTTTCCCC ACTGTCTCTT TTGTAGACAG GACTTTGGTG 
GAATCTGTAA CCCAGGCTCC AACTGCTCAA GAAGCAGGAG AAGGGCCTTC AGGCATTCTG 
GAACTTAGCG GTGCGCCTTC TGGAGCACCA GACATGTCTG GAGACCATTT GGGATCTTTG 
GACCAAAGTG GGCTTCAGTC TGGACTAGTG GAGCCCAGTG GGGAGCCCGC AAGTACTCCA 
TATTTTAGTG GGGACTTTTC TGGCACCACT GATGTAAGTG GGGAATCCTC TGCAGCCACG 
AGCACCAGTG GGGAGGCCTC CGGACTTCCA GAAGTTACGT TAATCACATC TGAGTTGGTG 
GAGGGAGTTA CTGAACCAAC CGTTTCCCAG GAACTGGGCC AGAGACCCCC CGTAACATAC 
ACCCCCCAGC TTTTTGAATC CAGTGGTGAA GCCTCTGCAT CTGGGGATGT GCCAAGGTTC 
CCTGGATCGG GGGTAGAAGT GTCATCAGTC CCAGAATCCA GCGGTGAGAC GTCAGCCTAT 
CCCGAGGCTG AGGTGGGAGC ATCTGCTGCC CCCGAGGCAA GCGGAGGAGC TTCCGGGTCC 
CCTAACCTGA GTGAAACCAC CTCCACCTTC CATGAAGCTG ATCTGGAGGG AACCTCAGGC 
CTGGGAGTGA GTGGCAGCCC CTCAGCCTTT CCAGAAGGCC CCACGGAGGG CTTGGCCACC 
CCGGAAGTGA GTGGAGAGTC AACCACTGCC TTTGACGTGA GCGTAGAGGC ATCAGGCTCA 
CCTTCTGCCA CTCCCCTGGC TTCTGGAGAC AGGACTGACA CCAGCGGAGA TCTGTCTGGC 
CACACCTCGG GGCTGGATAT TGTCATCAGC ACCACCATCC CAGAATCCGA GTGGACTCAG 
CAGACCCAGC GCCCTGCAGA GGCGCGTCTA GAAATCGAAT CCTCAAGCCC TGTGCACTCA 
GGAGAAGAGA GCCAAACAGC CGACACAGCC ACCTCCCCGA CTGATGCTTC TATCCCAGCC 
TCCGCAGGAG GGACAGATGA TTCAGAGGCA ACCACAACAG ACATTGACGA GTGCCTCTCA 
AGCCCTTGTC TGAATGGAGC CACCTGCGTG GATGCCATCG ACTCTTTCAC ATGCTTATGC 
CTTCCCAGCT ACCAAGGGGA CGTGTGTGAG ATTGACCAGA AGCTGTGCGA GGAGGGCTGG 
ACCAAGTTCC AGGGCCACTG TTACCGCCAC TTCCCCGACC GGGCAACCTG GGTGGACGCT 
GAGAGCCAGT GCCGGAAGCA GCAGTCACAC CTGAGCAGCA TCGTCACCCC CGAGGAGCAG 
GAGTTTGTCA ACAACAATGC CCAGGACTAC CAGTGGATCG GCCTGAACGA CAAGACCATC 
GAAGGGGACT TCCGCTGGTC AGATGGACAC TCCTTGCAAT TTGAGAACTG GCGCCCCAAC 
CAGCCTGACA ACTTCTTTGC CACTGGGGAG GACTGTGTGG TGATGATCTG GCATGAGAAG 
GGCGAGTGGA ACGATGTCCC ATGTAATTAC CAGCTACCCT TCACCTGTAA AAAGGGCACA 
GTGGCCTGCG GAGAGCCCCC CGTGGTGGAG CATGCCAGAA TCTTCGGACA GAAGAAGGAC 
CGATACGAGA TCAATGCCCT GGTGCGGTAC CAGTGCACCG AGGGCTTCAT CCAGCGCCAT 
GTGCCCACCA TCCGGTGCCA GCCCAGCGGG CACTGGGAGG AGCCTCGGAT CACCTGCACA 
GACCCCGCTA CCTACAAGCG CAGACTACAG AAGCGGAGCT CACGGCCCCT GAGGAGGAGC 
CACCCCAGCA CGGCCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837356.1
CDS	11..7111
Translation	MTTLLLVFVTLRVITAAISVEVSEPDNSLSVSIPEPSPLRVLLGSSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRISKEKEVVLLVATEGRVRVNSAYQDKVTLPNYPAIPSDATLEIQNMRSNDSGIYRCEVMHGIEDSQATLEVVVKGIVFHYRAISTRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPREGCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRLGARLATTGQLYLAWQGGMDMCSAGWLADRSVRYPISKARPNCGGNLLGVRTVYLHANQTGYPDPSSRYDAICYTGEDFVDIPESFFGVGGEEDITIQTVTWPDVELPLPRNITEGEARGSVILTAKPDFEVSPTAPEPEEPFTFVPEVRATAFPEVENRTEEATRPWAFPRESTPGLGAPTAFTSEDLVVQVTLAPGAAEVPGQPRLPGGVVFHYRPGSSRYSLTFEEAKQACLRTGAIIASPEQLQAAYEAGYEQCDAGWLQDQTVRYPIVSPRTPCVGDKDSSPGVRTYGVRPPSETYDVYCYVDRLEGEVFFATRLEQFTFWEAQEFCESQNATLATTGQLYAAWSRGLDKCYAGWLADGSLRYPIVTPRPACGGDKPGVRTVYLYPNQTGLLDPLSRHHAFCFRGVSAAPSPEEEEGSAPTAGPDVEEWMVTQVGPGVAAVPIGEETTAIPGFTVEPENKTEWELAYTPAGTLPLPGIPPTWPPTGEATEEHTEGPSATEVPSASEKPFPSEEPFPPEEPFPSEKPFPPEELFPSEKPFPSEKPFPSEEPFPSEKPFPPEELFPSEKPIPSEEPFPSEEPFPSEKPFPPEEPFPSEKPIPSEEPFPSEKPFPSEEPFPSEEPSTLSAPVPSRTELPSSGEVSGVPEISGDFTGSGEISGHLDFSGQPSGESASGLPSEDLDSSGLTSTVGSGLPVESGLPSGEEERITWTSAPKVDRLPSGGEGPEVSGVEDISGLPSGGEVHLEISASGVEDISGLPSGGEVHLEISASGVEDLSRIPSGEGPEISASGVEDISGLPSGEEGHLEISASGVEDLSGIPSGEGPEVSASGVEDLIGLPSGEGPEVSASGVEDLSRLPSGEGPEVSASGVEDLSGLPSGEGPEVSVSGVEDLSRLPSGEGPEVSASGVEDLSRLPSGEGPEISVSGVEDISILPSGEGPEVSASGVEDLSVLPSGEGHLEISTSGVEDLSVLPSGEGHLEISASGVEDISRLPSGEGPEVSASGVEDLSVLPSGEDHLEISASGVEDLGVLPSGEDHLEISASGVEDISRLPSGEGPEVSASGVEDLSVLPSGEGHLEISASGVEDLSRLPSGGEDHLETSASGVGDLSGLPSGREGLEISASGAGDLSGLTSGKEDLTGSASGALDLGRIPSVTLGSGQAPEASGLPSGFSGEYSGVDLESGPSSGLPDFSGLPSGFPTVSLVDTTLVEVVTATTAGELEGRGTIDISGAGETSGLPFSELDISGGASGLSSGAELSGQASGSPDISGETSGLFGVSGQPSGFPDISGETSGLLEVSGQPSGFYGEISGVTELSGLASGQPEISGEASGILSGLGPPFGITDLSGEAPGIPDLSGQPSGLPEFSGTTSGIPDLVSSAVSGSGESSGITFVDTSLVEVTPTTFKEEEGLGSVELSGLPSGEVGVSGTSGLADVSGLSSGAIDSSGFTSQPPEFSGLPSGVTEVSGEASGAESGSSLPSGAYDSSGLPSGFPTVSFVDRTLVESVTQAPTAQEAGEGPSGILELSGAPSGAPDMSGDHLGSLDQSGLQSGLVEPSGEPASTPYFSGDFSGTTDVSGESSAATSTSGEASGLPEVTLITSELVEGVTEPTVSQELGQRPPVTYTPQLFESSGEASASGDVPRFPGSGVEVSSVPESSGETSAYPEAEVGASAAPEASGGASGSPNLSETTSTFHEADLEGTSGLGVSGSPSAFPEGPTEGLATPEVSGESTTAFDVSVEASGSPSATPLASGDRTDTSGDLSGHTSGLDIVISTTIPESEWTQQTQRPAEARLEIESSSPVHSGEESQTADTATSPTDASIPASAGGTDDSEATTTDIDECLSSPCLNGATCVDAIDSFTCLCLPSYQGDVCEIDQKLCEEGWTKFQGHCYRHFPDRATWVDAESQCRKQQSHLSSIVTPEEQEFVNNNAQDYQWIGLNDKTIEGDFRWSDGHSLQFENWRPNQPDNFFATGEDCVVMIWHEKGEWNDVPCNYQLPFTCKKGTVACGEPPVVEHARIFGQKKDRYEINALVRYQCTEGFIQRHVPTIRCQPSGHWEEPRITCTDPATYKRRLQKRSSRPLRRSHPSTAH

Target ORF information:

RefSeq Version	XM_024981588.1
Organism	Bos taurus(cattle)
Definition	Bos taurus aggrecan (ACAN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981588.1

ORF Insert Sequence:

ATGACCACTT TACTCTTGGT GTTTGTGACT CTGAGGGTCA TCACAGCAGC CATCTCAGTA 
GAAGTTTCAG AACCTGACAA CTCGCTGAGT GTCAGCATCC CTGAACCGTC CCCGCTGCGG 
GTCCTCCTGG GGAGCTCCCT CACCATCCCC TGCTACTTCA TCGACCCCAT GCACCCCGTG 
ACCACCGCCC CCTCCACCGC CCCCCTTGCC CCAAGAATCA AGTGGAGCCG CATTTCCAAG 
GAGAAGGAGG TGGTGCTGCT GGTGGCCACT GAAGGGCGGG TGCGGGTCAA CAGCGCCTAC 
CAAGACAAGG TCACGCTGCC CAACTACCCC GCCATCCCCA GCGACGCCAC CCTGGAAATC 
CAGAACATGC GCTCCAATGA CTCCGGGATC TATCGCTGCG AGGTGATGCA CGGCATCGAG 
GACAGCCAGG CCACCCTAGA GGTCGTGGTA AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCCACGCGCT ACACCCTGGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGCGCC 
ATCATCGCCA CGCCCGAGCA GCTGCAGGCT GCCTATGAGG ACGGCTTCCA CCAGTGCGAC 
GCCGGCTGGC TGGCCGATCA GACTGTGAGG TATCCCATCC ACACGCCGAG GGAAGGTTGC 
TATGGAGACA AGGACGAGTT TCCCGGCGTG AGAACCTACG GCATCCGGGA CACCAACGAA 
ACCTATGACG TGTACTGCTT CGCGGAGGAG ATGGAGGGCG AGGTCTTCTA TGCAACATCC 
CCGGAGAAGT TCACCTTCCA AGAGGCAGCC AACGAGTGCC GGCGGCTGGG CGCCCGGCTG 
GCCACCACGG GCCAACTCTA CCTGGCCTGG CAGGGTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCTG ACCGCAGCGT GCGATACCCC ATCTCCAAGG CCCGGCCTAA CTGCGGGGGC 
AACCTCCTGG GAGTGAGGAC CGTCTACCTG CACGCCAACC AGACGGGCTA CCCTGACCCT 
TCATCCCGCT ATGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAGC 
TTTTTCGGGG TGGGCGGTGA GGAGGACATC ACCATCCAGA CGGTGACCTG GCCTGACGTG 
GAGCTGCCCC TGCCCCGAAA TATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTCACG 
GCAAAGCCCG ACTTTGAAGT CTCCCCCACC GCCCCGGAAC CCGAGGAGCC TTTCACGTTT 
GTCCCTGAAG TAAGGGCCAC TGCATTCCCC GAAGTAGAGA ACAGGACTGA AGAGGCCACC 
CGGCCCTGGG CCTTTCCCAG AGAGTCCACC CCCGGCCTGG GAGCCCCCAC GGCCTTCACC 
AGCGAGGACC TCGTCGTGCA GGTGACCTTA GCCCCAGGTG CGGCTGAGGT CCCTGGGCAG 
CCACGACTGC CAGGGGGAGT CGTGTTCCAC TACCGCCCAG GCTCCTCCCG CTACTCGCTG 
ACCTTTGAGG AGGCCAAGCA GGCCTGCCTG CGCACCGGGG CCATCATCGC CTCGCCCGAG 
CAGCTCCAGG CCGCCTATGA AGCAGGCTAC GAGCAGTGTG ACGCCGGCTG GCTGCAGGAC 
CAGACAGTCA GATACCCCAT TGTGAGCCCG CGGACCCCCT GTGTGGGTGA CAAGGACAGC 
AGCCCGGGGG TCCGGACCTA CGGCGTGCGG CCACCATCAG AAACCTACGA TGTCTACTGC 
TACGTGGACA GACTCGAGGG GGAGGTGTTC TTCGCCACAC GCCTGGAGCA GTTCACCTTC 
TGGGAAGCCC AGGAGTTCTG TGAATCCCAA AACGCCACTC TGGCCACCAC AGGCCAGCTC 
TATGCTGCCT GGAGCCGTGG TCTGGACAAG TGCTACGCCG GCTGGCTGGC TGACGGCAGC 
CTCCGCTACC CCATCGTCAC CCCAAGGCCC GCCTGTGGCG GGGACAAACC GGGCGTGAGA 
ACCGTCTACC TCTACCCCAA CCAGACGGGC CTCCTGGATC CGCTGTCCCG GCACCACGCC 
TTCTGCTTCC GAGGTGTTTC AGCGGCGCCC TCTCCAGAAG AAGAGGAGGG TAGTGCACCC 
ACAGCAGGCC CTGACGTGGA GGAGTGGATG GTGACACAAG TGGGGCCTGG CGTGGCTGCT 
GTCCCCATCG GGGAGGAGAC GACTGCAATC CCAGGCTTCA CCGTTGAGCC AGAAAACAAG 
ACGGAATGGG AACTTGCCTA CACCCCAGCG GGCACTTTGC CACTACCAGG GATCCCTCCT 
ACATGGCCTC CCACTGGAGA AGCAACAGAG GAGCACACAG AAGGCCCTTC TGCAACGGAA 
GTGCCCTCAG CCTCAGAGAA GCCGTTCCCT TCAGAGGAAC CATTCCCCCC AGAGGAACCA 
TTCCCCTCAG AGAAACCATT CCCCCCAGAG GAACTGTTCC CCTCAGAGAA GCCATTCCCC 
TCAGAAAAGC CGTTCCCCTC AGAGGAACCG TTCCCCTCAG AAAAACCATT CCCCCCAGAG 
GAACTGTTCC CCTCAGAGAA GCCAATCCCC TCAGAGGAGC CATTCCCCTC AGAGGAGCCA 
TTCCCCTCAG AGAAGCCATT CCCCCCAGAG GAACCATTCC CCTCAGAGAA ACCAATCCCC 
TCAGAGGAGC CTTTCCCCTC AGAGAAGCCA TTCCCTTCAG AGGAGCCATT CCCCTCAGAG 
GAGCCATCCA CACTTTCAGC CCCAGTGCCC AGCAGGACTG AGCTGCCAAG CTCTGGGGAG 
GTGTCTGGGG TGCCCGAAAT CAGTGGTGAC TTCACAGGCA GTGGAGAAAT TTCAGGACAC 
CTAGACTTCA GTGGGCAGCC CTCAGGGGAA AGTGCAAGTG GACTGCCCTC TGAAGACCTT 
GACTCCAGTG GGCTCACCTC TACAGTGGGC TCAGGCCTGC CTGTGGAAAG TGGACTGCCT 
TCAGGGGAAG AAGAGAGAAT TACATGGACC AGTGCTCCTA AAGTTGACAG GTTGCCCTCT 
GGGGGTGAGG GCCCAGAAGT TTCTGGAGTA GAGGATATCA GTGGACTTCC TTCTGGAGGA 
GAGGTTCATC TAGAGATCTC TGCCTCTGGA GTAGAGGACA TCAGTGGACT TCCTTCTGGA 
GGAGAGGTTC ATCTAGAGAT CTCTGCCTCT GGAGTAGAGG ACCTAAGTAG AATTCCTTCT 
GGAGAAGGTC CAGAAATCTC TGCCTCTGGA GTAGAGGACA TCAGTGGACT TCCTTCTGGA 
GAGGAAGGTC ATCTAGAGAT CTCTGCCTCT GGAGTAGAAG ACCTCAGTGG AATTCCTTCT 
GGAGAAGGTC CAGAAGTCTC TGCTTCTGGA GTAGAGGACC TTATTGGACT TCCTTCTGGA 
GAAGGTCCAG AAGTCTCTGC TTCTGGAGTA GAGGACCTCA GTAGACTTCC TTCTGGAGAA 
GGTCCAGAAG TCTCTGCTTC TGGGGTAGAG GACCTTAGTG GACTTCCTTC TGGAGAAGGT 
CCAGAAGTCT CTGTTTCTGG AGTAGAGGAT CTCAGCAGAC TTCCTTCTGG AGAAGGTCCA 
GAAGTCTCTG CTTCTGGAGT AGAGGACCTC AGTAGACTTC CTTCTGGAGA AGGTCCAGAA 
ATCTCTGTGT CTGGAGTAGA GGACATCAGT ATACTTCCTT CTGGAGAAGG TCCAGAAGTC 
TCTGCCTCTG GAGTAGAGGA CCTCAGTGTT CTTCCTTCTG GAGAAGGTCA TCTAGAGATC 
TCTACCTCTG GAGTAGAGGA CCTCAGTGTT CTTCCTTCTG GTGAAGGTCA TCTAGAGATC 
TCTGCCTCTG GAGTAGAGGA CATCAGTAGA CTTCCTTCTG GAGAAGGTCC AGAAGTCTCT 
GCCTCTGGAG TAGAGGACCT CAGTGTTCTT CCTTCTGGAG AAGATCATCT AGAGATCTCT 
GCCTCTGGAG TAGAGGACCT CGGTGTTCTT CCTTCTGGAG AAGATCATCT AGAGATCTCT 
GCCTCTGGAG TAGAGGACAT CAGTAGACTT CCTTCTGGAG AAGGTCCAGA AGTCTCTGCC 
TCTGGAGTAG AGGACCTCAG TGTTCTTCCT TCTGGAGAAG GTCATCTAGA GATCTCTGCC 
TCTGGAGTAG AGGACCTCAG TAGACTTCCT TCTGGAGGAG AGGATCATCT AGAGACTTCT 
GCTTCTGGAG TAGGGGACCT TAGTGGACTT CCTTCTGGAA GGGAAGGTCT GGAGATCTCT 
GCTTCTGGAG CTGGGGATCT TAGTGGGTTG ACTTCTGGAA AAGAAGACTT GACTGGGTCA 
GCTTCTGGAG CCTTGGACCT TGGCAGAATA CCTTCTGTAA CTCTAGGAAG TGGGCAAGCT 
CCAGAAGCAA GTGGTCTTCC TTCTGGATTT AGTGGTGAGT ATTCTGGGGT GGACCTTGAA 
AGTGGCCCAT CCTCTGGCCT TCCTGATTTC AGTGGACTTC CATCTGGGTT CCCAACTGTC 
TCCCTAGTGG ATACTACATT GGTGGAAGTC GTCACAGCCA CCACAGCAGG TGAACTAGAA 
GGAAGGGGAA CCATTGACAT CAGCGGTGCT GGAGAAACAT CTGGGCTGCC CTTCAGTGAG 
TTGGACATTA GTGGAGGAGC AAGCGGACTC TCTTCAGGAG CTGAACTCAG TGGCCAAGCA 
TCTGGGTCTC CTGACATCAG TGGGGAAACC TCTGGACTCT TTGGTGTCAG TGGACAGCCC 
TCAGGGTTTC CTGACATTAG TGGGGAAACT TCCGGGCTTC TTGAGGTCAG TGGGCAGCCA 
TCAGGGTTTT ATGGAGAAAT ATCTGGCGTG ACTGAGCTTA GCGGACTGGC CTCTGGACAA 
CCAGAAATCA GTGGAGAAGC TTCTGGAATT CTTTCTGGCC TTGGTCCACC ATTCGGCATA 
ACCGACCTGA GCGGAGAAGC ACCCGGGATC CCTGATCTCA GTGGGCAACC ATCGGGCTTG 
CCAGAGTTCA GTGGGACAAC ATCCGGGATC CCTGACCTGG TTTCCAGTGC TGTGAGTGGC 
AGTGGTGAAT CTTCTGGCAT TACGTTCGTG GACACCAGTT TGGTTGAAGT GACCCCAACG 
ACATTTAAAG AAGAAGAAGG CTTAGGATCT GTGGAACTCA GTGGCCTCCC TTCAGGAGAG 
GTGGGTGTCT CAGGCACATC TGGGCTAGCG GATGTCAGTG GACTGTCTTC TGGAGCAATT 
GACTCCAGTG GGTTTACGTC CCAGCCTCCA GAATTCAGTG GCCTGCCAAG CGGAGTAACT 
GAGGTCAGTG GAGAAGCCTC CGGAGCTGAA AGTGGGAGCA GCCTGCCCTC CGGAGCATAT 
GACAGCAGTG GACTTCCGTC TGGTTTCCCC ACTGTCTCTT TTGTAGACAG GACTTTGGTG 
GAATCTGTAA CCCAGGCTCC AACTGCTCAA GAAGCAGGAG AAGGGCCTTC AGGCATTCTG 
GAACTTAGCG GTGCGCCTTC TGGAGCACCA GACATGTCTG GAGACCATTT GGGATCTTTG 
GACCAAAGTG GGCTTCAGTC TGGACTAGTG GAGCCCAGTG GGGAGCCCGC AAGTACTCCA 
TATTTTAGTG GGGACTTTTC TGGCACCACT GATGTAAGTG GGGAATCCTC TGCAGCCACG 
AGCACCAGTG GGGAGGCCTC CGGACTTCCA GAAGTTACGT TAATCACATC TGAGTTGGTG 
GAGGGAGTTA CTGAACCAAC CGTTTCCCAG GAACTGGGCC AGAGACCCCC CGTAACATAC 
ACCCCCCAGC TTTTTGAATC CAGTGGTGAA GCCTCTGCAT CTGGGGATGT GCCAAGGTTC 
CCTGGATCGG GGGTAGAAGT GTCATCAGTC CCAGAATCCA GCGGTGAGAC GTCAGCCTAT 
CCCGAGGCTG AGGTGGGAGC ATCTGCTGCC CCCGAGGCAA GCGGAGGAGC TTCCGGGTCC 
CCTAACCTGA GTGAAACCAC CTCCACCTTC CATGAAGCTG ATCTGGAGGG AACCTCAGGC 
CTGGGAGTGA GTGGCAGCCC CTCAGCCTTT CCAGAAGGCC CCACGGAGGG CTTGGCCACC 
CCGGAAGTGA GTGGAGAGTC AACCACTGCC TTTGACGTGA GCGTAGAGGC ATCAGGCTCA 
CCTTCTGCCA CTCCCCTGGC TTCTGGAGAC AGGACTGACA CCAGCGGAGA TCTGTCTGGC 
CACACCTCGG GGCTGGATAT TGTCATCAGC ACCACCATCC CAGAATCCGA GTGGACTCAG 
CAGACCCAGC GCCCTGCAGA GGCGCGTCTA GAAATCGAAT CCTCAAGCCC TGTGCACTCA 
GGAGAAGAGA GCCAAACAGC CGACACAGCC ACCTCCCCGA CTGATGCTTC TATCCCAGCC 
TCCGCAGGAG GGACAGATGA TTCAGAGGCA ACCACAACAG ACATTGACGA GTGCCTCTCA 
AGCCCTTGTC TGAATGGAGC CACCTGCGTG GATGCCATCG ACTCTTTCAC ATGCTTATGC 
CTTCCCAGCT ACCAAGGGGA CGTGTGTGAG ATTGACCAGA AGCTGTGCGA GGAGGGCTGG 
ACCAAGTTCC AGGGCCACTG TTACCGCCAC TTCCCCGACC GGGCAACCTG GGTGGACGCT 
GAGAGCCAGT GCCGGAAGCA GCAGTCACAC CTGAGCAGCA TCGTCACCCC CGAGGAGCAG 
GAGTTTGTCA ACAACAATGC CCAGGACTAC CAGTGGATCG GCCTGAACGA CAAGACCATC 
GAAGGGGACT TCCGCTGGTC AGATGGACAC TCCTTGCAAT TTGAGAACTG GCGCCCCAAC 
CAGCCTGACA ACTTCTTTGC CACTGGGGAG GACTGTGTGG TGATGATCTG GCATGAGAAG 
GGCGAGTGGA ACGATGTCCC ATGTAATTAC CAGCTACCCT TCACCTGTAA AAAGGGCACA 
GTGGCCTGCG GAGAGCCCCC CGTGGTGGAG CATGCCAGAA TCTTCGGACA GAAGAAGGAC 
CGATACGAGA TCAATGCCCT GGTGCGGTAC CAGTGCACCG AGGGCTTCAT CCAGCGCCAT 
GTGCCCACCA TCCGGTGCCA GCCCAGCGGG CACTGGGAGG AGCCTCGGAT CACCTGCACA 
GACCCCGCTA CCTACAAGCG CAGACTACAG AAGCGGAGCT CACGGCCCCT GAGGAGGAGC 
CACCCCAGCA CGGCCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACAN ( XM_005221803.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221860.1 Bos taurus aggrecan (ACAN), transcript variant X1, mRNA.

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CloneID	OBa30830
Clone ID Related Accession (Same CDS sequence)	XM_024981588.1 , XM_005221803.3
Accession Version	XM_005221803.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	aggrecan core protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104533.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221803.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTT TACTCTTGGT GTTTGTGACT CTGAGGGTCA TCACAGCAGC CATCTCAGTA 
GAAGTTTCAG AACCTGACAA CTCGCTGAGT GTCAGCATCC CTGAACCGTC CCCGCTGCGG 
GTCCTCCTGG GGAGCTCCCT CACCATCCCC TGCTACTTCA TCGACCCCAT GCACCCCGTG 
ACCACCGCCC CCTCCACCGC CCCCCTTGCC CCAAGAATCA AGTGGAGCCG CATTTCCAAG 
GAGAAGGAGG TGGTGCTGCT GGTGGCCACT GAAGGGCGGG TGCGGGTCAA CAGCGCCTAC 
CAAGACAAGG TCACGCTGCC CAACTACCCC GCCATCCCCA GCGACGCCAC CCTGGAAATC 
CAGAACATGC GCTCCAATGA CTCCGGGATC TATCGCTGCG AGGTGATGCA CGGCATCGAG 
GACAGCCAGG CCACCCTAGA GGTCGTGGTA AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCCACGCGCT ACACCCTGGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGCGCC 
ATCATCGCCA CGCCCGAGCA GCTGCAGGCT GCCTATGAGG ACGGCTTCCA CCAGTGCGAC 
GCCGGCTGGC TGGCCGATCA GACTGTGAGG TATCCCATCC ACACGCCGAG GGAAGGTTGC 
TATGGAGACA AGGACGAGTT TCCCGGCGTG AGAACCTACG GCATCCGGGA CACCAACGAA 
ACCTATGACG TGTACTGCTT CGCGGAGGAG ATGGAGGGCG AGGTCTTCTA TGCAACATCC 
CCGGAGAAGT TCACCTTCCA AGAGGCAGCC AACGAGTGCC GGCGGCTGGG CGCCCGGCTG 
GCCACCACGG GCCAACTCTA CCTGGCCTGG CAGGGTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCTG ACCGCAGCGT GCGATACCCC ATCTCCAAGG CCCGGCCTAA CTGCGGGGGC 
AACCTCCTGG GAGTGAGGAC CGTCTACCTG CACGCCAACC AGACGGGCTA CCCTGACCCT 
TCATCCCGCT ATGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAGC 
TTTTTCGGGG TGGGCGGTGA GGAGGACATC ACCATCCAGA CGGTGACCTG GCCTGACGTG 
GAGCTGCCCC TGCCCCGAAA TATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTCACG 
GCAAAGCCCG ACTTTGAAGT CTCCCCCACC GCCCCGGAAC CCGAGGAGCC TTTCACGTTT 
GTCCCTGAAG TAAGGGCCAC TGCATTCCCC GAAGTAGAGA ACAGGACTGA AGAGGCCACC 
CGGCCCTGGG CCTTTCCCAG AGAGTCCACC CCCGGCCTGG GAGCCCCCAC GGCCTTCACC 
AGCGAGGACC TCGTCGTGCA GGTGACCTTA GCCCCAGGTG CGGCTGAGGT CCCTGGGCAG 
CCACGACTGC CAGGGGGAGT CGTGTTCCAC TACCGCCCAG GCTCCTCCCG CTACTCGCTG 
ACCTTTGAGG AGGCCAAGCA GGCCTGCCTG CGCACCGGGG CCATCATCGC CTCGCCCGAG 
CAGCTCCAGG CCGCCTATGA AGCAGGCTAC GAGCAGTGTG ACGCCGGCTG GCTGCAGGAC 
CAGACAGTCA GATACCCCAT TGTGAGCCCG CGGACCCCCT GTGTGGGTGA CAAGGACAGC 
AGCCCGGGGG TCCGGACCTA CGGCGTGCGG CCACCATCAG AAACCTACGA TGTCTACTGC 
TACGTGGACA GACTCGAGGG GGAGGTGTTC TTCGCCACAC GCCTGGAGCA GTTCACCTTC 
TGGGAAGCCC AGGAGTTCTG TGAATCCCAA AACGCCACTC TGGCCACCAC AGGCCAGCTC 
TATGCTGCCT GGAGCCGTGG TCTGGACAAG TGCTACGCCG GCTGGCTGGC TGACGGCAGC 
CTCCGCTACC CCATCGTCAC CCCAAGGCCC GCCTGTGGCG GGGACAAACC GGGCGTGAGA 
ACCGTCTACC TCTACCCCAA CCAGACGGGC CTCCTGGATC CGCTGTCCCG GCACCACGCC 
TTCTGCTTCC GAGGTGTTTC AGCGGCGCCC TCTCCAGAAG AAGAGGAGGG TAGTGCACCC 
ACAGCAGGCC CTGACGTGGA GGAGTGGATG GTGACACAAG TGGGGCCTGG CGTGGCTGCT 
GTCCCCATCG GGGAGGAGAC GACTGCAATC CCAGGCTTCA CCGTTGAGCC AGAAAACAAG 
ACGGAATGGG AACTTGCCTA CACCCCAGCG GGCACTTTGC CACTACCAGG GATCCCTCCT 
ACATGGCCTC CCACTGGAGA AGCAACAGAG GAGCACACAG AAGGCCCTTC TGCAACGGAA 
GTGCCCTCAG CCTCAGAGAA GCCGTTCCCT TCAGAGGAAC CATTCCCCCC AGAGGAACCA 
TTCCCCTCAG AGAAACCATT CCCCCCAGAG GAACTGTTCC CCTCAGAGAA GCCATTCCCC 
TCAGAAAAGC CGTTCCCCTC AGAGGAACCG TTCCCCTCAG AAAAACCATT CCCCCCAGAG 
GAACTGTTCC CCTCAGAGAA GCCAATCCCC TCAGAGGAGC CATTCCCCTC AGAGGAGCCA 
TTCCCCTCAG AGAAGCCATT CCCCCCAGAG GAACCATTCC CCTCAGAGAA ACCAATCCCC 
TCAGAGGAGC CTTTCCCCTC AGAGAAGCCA TTCCCTTCAG AGGAGCCATT CCCCTCAGAG 
GAGCCATCCA CACTTTCAGC CCCAGTGCCC AGCAGGACTG AGCTGCCAAG CTCTGGGGAG 
GTGTCTGGGG TGCCCGAAAT CAGTGGTGAC TTCACAGGCA GTGGAGAAAT TTCAGGACAC 
CTAGACTTCA GTGGGCAGCC CTCAGGGGAA AGTGCAAGTG GACTGCCCTC TGAAGACCTT 
GACTCCAGTG GGCTCACCTC TACAGTGGGC TCAGGCCTGC CTGTGGAAAG TGGACTGCCT 
TCAGGGGAAG AAGAGAGAAT TACATGGACC AGTGCTCCTA AAGTTGACAG GTTGCCCTCT 
GGGGGTGAGG GCCCAGAAGT TTCTGGAGTA GAGGATATCA GTGGACTTCC TTCTGGAGGA 
GAGGTTCATC TAGAGATCTC TGCCTCTGGA GTAGAGGACA TCAGTGGACT TCCTTCTGGA 
GGAGAGGTTC ATCTAGAGAT CTCTGCCTCT GGAGTAGAGG ACCTAAGTAG AATTCCTTCT 
GGAGAAGGTC CAGAAATCTC TGCCTCTGGA GTAGAGGACA TCAGTGGACT TCCTTCTGGA 
GAGGAAGGTC ATCTAGAGAT CTCTGCCTCT GGAGTAGAAG ACCTCAGTGG AATTCCTTCT 
GGAGAAGGTC CAGAAGTCTC TGCTTCTGGA GTAGAGGACC TTATTGGACT TCCTTCTGGA 
GAAGGTCCAG AAGTCTCTGC TTCTGGAGTA GAGGACCTCA GTAGACTTCC TTCTGGAGAA 
GGTCCAGAAG TCTCTGCTTC TGGGGTAGAG GACCTTAGTG GACTTCCTTC TGGAGAAGGT 
CCAGAAGTCT CTGTTTCTGG AGTAGAGGAT CTCAGCAGAC TTCCTTCTGG AGAAGGTCCA 
GAAGTCTCTG CTTCTGGAGT AGAGGACCTC AGTAGACTTC CTTCTGGAGA AGGTCCAGAA 
ATCTCTGTGT CTGGAGTAGA GGACATCAGT ATACTTCCTT CTGGAGAAGG TCCAGAAGTC 
TCTGCCTCTG GAGTAGAGGA CCTCAGTGTT CTTCCTTCTG GAGAAGGTCA TCTAGAGATC 
TCTACCTCTG GAGTAGAGGA CCTCAGTGTT CTTCCTTCTG GTGAAGGTCA TCTAGAGATC 
TCTGCCTCTG GAGTAGAGGA CATCAGTAGA CTTCCTTCTG GAGAAGGTCC AGAAGTCTCT 
GCCTCTGGAG TAGAGGACCT CAGTGTTCTT CCTTCTGGAG AAGATCATCT AGAGATCTCT 
GCCTCTGGAG TAGAGGACCT CGGTGTTCTT CCTTCTGGAG AAGATCATCT AGAGATCTCT 
GCCTCTGGAG TAGAGGACAT CAGTAGACTT CCTTCTGGAG AAGGTCCAGA AGTCTCTGCC 
TCTGGAGTAG AGGACCTCAG TGTTCTTCCT TCTGGAGAAG GTCATCTAGA GATCTCTGCC 
TCTGGAGTAG AGGACCTCAG TAGACTTCCT TCTGGAGGAG AGGATCATCT AGAGACTTCT 
GCTTCTGGAG TAGGGGACCT TAGTGGACTT CCTTCTGGAA GGGAAGGTCT GGAGATCTCT 
GCTTCTGGAG CTGGGGATCT TAGTGGGTTG ACTTCTGGAA AAGAAGACTT GACTGGGTCA 
GCTTCTGGAG CCTTGGACCT TGGCAGAATA CCTTCTGTAA CTCTAGGAAG TGGGCAAGCT 
CCAGAAGCAA GTGGTCTTCC TTCTGGATTT AGTGGTGAGT ATTCTGGGGT GGACCTTGAA 
AGTGGCCCAT CCTCTGGCCT TCCTGATTTC AGTGGACTTC CATCTGGGTT CCCAACTGTC 
TCCCTAGTGG ATACTACATT GGTGGAAGTC GTCACAGCCA CCACAGCAGG TGAACTAGAA 
GGAAGGGGAA CCATTGACAT CAGCGGTGCT GGAGAAACAT CTGGGCTGCC CTTCAGTGAG 
TTGGACATTA GTGGAGGAGC AAGCGGACTC TCTTCAGGAG CTGAACTCAG TGGCCAAGCA 
TCTGGGTCTC CTGACATCAG TGGGGAAACC TCTGGACTCT TTGGTGTCAG TGGACAGCCC 
TCAGGGTTTC CTGACATTAG TGGGGAAACT TCCGGGCTTC TTGAGGTCAG TGGGCAGCCA 
TCAGGGTTTT ATGGAGAAAT ATCTGGCGTG ACTGAGCTTA GCGGACTGGC CTCTGGACAA 
CCAGAAATCA GTGGAGAAGC TTCTGGAATT CTTTCTGGCC TTGGTCCACC ATTCGGCATA 
ACCGACCTGA GCGGAGAAGC ACCCGGGATC CCTGATCTCA GTGGGCAACC ATCGGGCTTG 
CCAGAGTTCA GTGGGACAAC ATCCGGGATC CCTGACCTGG TTTCCAGTGC TGTGAGTGGC 
AGTGGTGAAT CTTCTGGCAT TACGTTCGTG GACACCAGTT TGGTTGAAGT GACCCCAACG 
ACATTTAAAG AAGAAGAAGG CTTAGGATCT GTGGAACTCA GTGGCCTCCC TTCAGGAGAG 
GTGGGTGTCT CAGGCACATC TGGGCTAGCG GATGTCAGTG GACTGTCTTC TGGAGCAATT 
GACTCCAGTG GGTTTACGTC CCAGCCTCCA GAATTCAGTG GCCTGCCAAG CGGAGTAACT 
GAGGTCAGTG GAGAAGCCTC CGGAGCTGAA AGTGGGAGCA GCCTGCCCTC CGGAGCATAT 
GACAGCAGTG GACTTCCGTC TGGTTTCCCC ACTGTCTCTT TTGTAGACAG GACTTTGGTG 
GAATCTGTAA CCCAGGCTCC AACTGCTCAA GAAGCAGGAG AAGGGCCTTC AGGCATTCTG 
GAACTTAGCG GTGCGCCTTC TGGAGCACCA GACATGTCTG GAGACCATTT GGGATCTTTG 
GACCAAAGTG GGCTTCAGTC TGGACTAGTG GAGCCCAGTG GGGAGCCCGC AAGTACTCCA 
TATTTTAGTG GGGACTTTTC TGGCACCACT GATGTAAGTG GGGAATCCTC TGCAGCCACG 
AGCACCAGTG GGGAGGCCTC CGGACTTCCA GAAGTTACGT TAATCACATC TGAGTTGGTG 
GAGGGAGTTA CTGAACCAAC CGTTTCCCAG GAACTGGGCC AGAGACCCCC CGTAACATAC 
ACCCCCCAGC TTTTTGAATC CAGTGGTGAA GCCTCTGCAT CTGGGGATGT GCCAAGGTTC 
CCTGGATCGG GGGTAGAAGT GTCATCAGTC CCAGAATCCA GCGGTGAGAC GTCAGCCTAT 
CCCGAGGCTG AGGTGGGAGC ATCTGCTGCC CCCGAGGCAA GCGGAGGAGC TTCCGGGTCC 
CCTAACCTGA GTGAAACCAC CTCCACCTTC CATGAAGCTG ATCTGGAGGG AACCTCAGGC 
CTGGGAGTGA GTGGCAGCCC CTCAGCCTTT CCAGAAGGCC CCACGGAGGG CTTGGCCACC 
CCGGAAGTGA GTGGAGAGTC AACCACTGCC TTTGACGTGA GCGTAGAGGC ATCAGGCTCA 
CCTTCTGCCA CTCCCCTGGC TTCTGGAGAC AGGACTGACA CCAGCGGAGA TCTGTCTGGC 
CACACCTCGG GGCTGGATAT TGTCATCAGC ACCACCATCC CAGAATCCGA GTGGACTCAG 
CAGACCCAGC GCCCTGCAGA GGCGCGTCTA GAAATCGAAT CCTCAAGCCC TGTGCACTCA 
GGAGAAGAGA GCCAAACAGC CGACACAGCC ACCTCCCCGA CTGATGCTTC TATCCCAGCC 
TCCGCAGGAG GGACAGATGA TTCAGAGGCA ACCACAACAG ACATTGACGA GTGCCTCTCA 
AGCCCTTGTC TGAATGGAGC CACCTGCGTG GATGCCATCG ACTCTTTCAC ATGCTTATGC 
CTTCCCAGCT ACCAAGGGGA CGTGTGTGAG ATTGACCAGA AGCTGTGCGA GGAGGGCTGG 
ACCAAGTTCC AGGGCCACTG TTACCGCCAC TTCCCCGACC GGGCAACCTG GGTGGACGCT 
GAGAGCCAGT GCCGGAAGCA GCAGTCACAC CTGAGCAGCA TCGTCACCCC CGAGGAGCAG 
GAGTTTGTCA ACAACAATGC CCAGGACTAC CAGTGGATCG GCCTGAACGA CAAGACCATC 
GAAGGGGACT TCCGCTGGTC AGATGGACAC TCCTTGCAAT TTGAGAACTG GCGCCCCAAC 
CAGCCTGACA ACTTCTTTGC CACTGGGGAG GACTGTGTGG TGATGATCTG GCATGAGAAG 
GGCGAGTGGA ACGATGTCCC ATGTAATTAC CAGCTACCCT TCACCTGTAA AAAGGGCACA 
GTGGCCTGCG GAGAGCCCCC CGTGGTGGAG CATGCCAGAA TCTTCGGACA GAAGAAGGAC 
CGATACGAGA TCAATGCCCT GGTGCGGTAC CAGTGCACCG AGGGCTTCAT CCAGCGCCAT 
GTGCCCACCA TCCGGTGCCA GCCCAGCGGG CACTGGGAGG AGCCTCGGAT CACCTGCACA 
GACCCCGCTA CCTACAAGCG CAGACTACAG AAGCGGAGCT CACGGCCCCT GAGGAGGAGC 
CACCCCAGCA CGGCCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221860.1
CDS	339..7439
Translation	MTTLLLVFVTLRVITAAISVEVSEPDNSLSVSIPEPSPLRVLLGSSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRISKEKEVVLLVATEGRVRVNSAYQDKVTLPNYPAIPSDATLEIQNMRSNDSGIYRCEVMHGIEDSQATLEVVVKGIVFHYRAISTRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPREGCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRLGARLATTGQLYLAWQGGMDMCSAGWLADRSVRYPISKARPNCGGNLLGVRTVYLHANQTGYPDPSSRYDAICYTGEDFVDIPESFFGVGGEEDITIQTVTWPDVELPLPRNITEGEARGSVILTAKPDFEVSPTAPEPEEPFTFVPEVRATAFPEVENRTEEATRPWAFPRESTPGLGAPTAFTSEDLVVQVTLAPGAAEVPGQPRLPGGVVFHYRPGSSRYSLTFEEAKQACLRTGAIIASPEQLQAAYEAGYEQCDAGWLQDQTVRYPIVSPRTPCVGDKDSSPGVRTYGVRPPSETYDVYCYVDRLEGEVFFATRLEQFTFWEAQEFCESQNATLATTGQLYAAWSRGLDKCYAGWLADGSLRYPIVTPRPACGGDKPGVRTVYLYPNQTGLLDPLSRHHAFCFRGVSAAPSPEEEEGSAPTAGPDVEEWMVTQVGPGVAAVPIGEETTAIPGFTVEPENKTEWELAYTPAGTLPLPGIPPTWPPTGEATEEHTEGPSATEVPSASEKPFPSEEPFPPEEPFPSEKPFPPEELFPSEKPFPSEKPFPSEEPFPSEKPFPPEELFPSEKPIPSEEPFPSEEPFPSEKPFPPEEPFPSEKPIPSEEPFPSEKPFPSEEPFPSEEPSTLSAPVPSRTELPSSGEVSGVPEISGDFTGSGEISGHLDFSGQPSGESASGLPSEDLDSSGLTSTVGSGLPVESGLPSGEEERITWTSAPKVDRLPSGGEGPEVSGVEDISGLPSGGEVHLEISASGVEDISGLPSGGEVHLEISASGVEDLSRIPSGEGPEISASGVEDISGLPSGEEGHLEISASGVEDLSGIPSGEGPEVSASGVEDLIGLPSGEGPEVSASGVEDLSRLPSGEGPEVSASGVEDLSGLPSGEGPEVSVSGVEDLSRLPSGEGPEVSASGVEDLSRLPSGEGPEISVSGVEDISILPSGEGPEVSASGVEDLSVLPSGEGHLEISTSGVEDLSVLPSGEGHLEISASGVEDISRLPSGEGPEVSASGVEDLSVLPSGEDHLEISASGVEDLGVLPSGEDHLEISASGVEDISRLPSGEGPEVSASGVEDLSVLPSGEGHLEISASGVEDLSRLPSGGEDHLETSASGVGDLSGLPSGREGLEISASGAGDLSGLTSGKEDLTGSASGALDLGRIPSVTLGSGQAPEASGLPSGFSGEYSGVDLESGPSSGLPDFSGLPSGFPTVSLVDTTLVEVVTATTAGELEGRGTIDISGAGETSGLPFSELDISGGASGLSSGAELSGQASGSPDISGETSGLFGVSGQPSGFPDISGETSGLLEVSGQPSGFYGEISGVTELSGLASGQPEISGEASGILSGLGPPFGITDLSGEAPGIPDLSGQPSGLPEFSGTTSGIPDLVSSAVSGSGESSGITFVDTSLVEVTPTTFKEEEGLGSVELSGLPSGEVGVSGTSGLADVSGLSSGAIDSSGFTSQPPEFSGLPSGVTEVSGEASGAESGSSLPSGAYDSSGLPSGFPTVSFVDRTLVESVTQAPTAQEAGEGPSGILELSGAPSGAPDMSGDHLGSLDQSGLQSGLVEPSGEPASTPYFSGDFSGTTDVSGESSAATSTSGEASGLPEVTLITSELVEGVTEPTVSQELGQRPPVTYTPQLFESSGEASASGDVPRFPGSGVEVSSVPESSGETSAYPEAEVGASAAPEASGGASGSPNLSETTSTFHEADLEGTSGLGVSGSPSAFPEGPTEGLATPEVSGESTTAFDVSVEASGSPSATPLASGDRTDTSGDLSGHTSGLDIVISTTIPESEWTQQTQRPAEARLEIESSSPVHSGEESQTADTATSPTDASIPASAGGTDDSEATTTDIDECLSSPCLNGATCVDAIDSFTCLCLPSYQGDVCEIDQKLCEEGWTKFQGHCYRHFPDRATWVDAESQCRKQQSHLSSIVTPEEQEFVNNNAQDYQWIGLNDKTIEGDFRWSDGHSLQFENWRPNQPDNFFATGEDCVVMIWHEKGEWNDVPCNYQLPFTCKKGTVACGEPPVVEHARIFGQKKDRYEINALVRYQCTEGFIQRHVPTIRCQPSGHWEEPRITCTDPATYKRRLQKRSSRPLRRSHPSTAH

Target ORF information:

RefSeq Version	XM_005221803.3
Organism	Bos taurus(cattle)
Definition	Bos taurus aggrecan (ACAN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221803.3

ORF Insert Sequence:

ATGACCACTT TACTCTTGGT GTTTGTGACT CTGAGGGTCA TCACAGCAGC CATCTCAGTA 
GAAGTTTCAG AACCTGACAA CTCGCTGAGT GTCAGCATCC CTGAACCGTC CCCGCTGCGG 
GTCCTCCTGG GGAGCTCCCT CACCATCCCC TGCTACTTCA TCGACCCCAT GCACCCCGTG 
ACCACCGCCC CCTCCACCGC CCCCCTTGCC CCAAGAATCA AGTGGAGCCG CATTTCCAAG 
GAGAAGGAGG TGGTGCTGCT GGTGGCCACT GAAGGGCGGG TGCGGGTCAA CAGCGCCTAC 
CAAGACAAGG TCACGCTGCC CAACTACCCC GCCATCCCCA GCGACGCCAC CCTGGAAATC 
CAGAACATGC GCTCCAATGA CTCCGGGATC TATCGCTGCG AGGTGATGCA CGGCATCGAG 
GACAGCCAGG CCACCCTAGA GGTCGTGGTA AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCCACGCGCT ACACCCTGGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGCGCC 
ATCATCGCCA CGCCCGAGCA GCTGCAGGCT GCCTATGAGG ACGGCTTCCA CCAGTGCGAC 
GCCGGCTGGC TGGCCGATCA GACTGTGAGG TATCCCATCC ACACGCCGAG GGAAGGTTGC 
TATGGAGACA AGGACGAGTT TCCCGGCGTG AGAACCTACG GCATCCGGGA CACCAACGAA 
ACCTATGACG TGTACTGCTT CGCGGAGGAG ATGGAGGGCG AGGTCTTCTA TGCAACATCC 
CCGGAGAAGT TCACCTTCCA AGAGGCAGCC AACGAGTGCC GGCGGCTGGG CGCCCGGCTG 
GCCACCACGG GCCAACTCTA CCTGGCCTGG CAGGGTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCTG ACCGCAGCGT GCGATACCCC ATCTCCAAGG CCCGGCCTAA CTGCGGGGGC 
AACCTCCTGG GAGTGAGGAC CGTCTACCTG CACGCCAACC AGACGGGCTA CCCTGACCCT 
TCATCCCGCT ATGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAGC 
TTTTTCGGGG TGGGCGGTGA GGAGGACATC ACCATCCAGA CGGTGACCTG GCCTGACGTG 
GAGCTGCCCC TGCCCCGAAA TATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTCACG 
GCAAAGCCCG ACTTTGAAGT CTCCCCCACC GCCCCGGAAC CCGAGGAGCC TTTCACGTTT 
GTCCCTGAAG TAAGGGCCAC TGCATTCCCC GAAGTAGAGA ACAGGACTGA AGAGGCCACC 
CGGCCCTGGG CCTTTCCCAG AGAGTCCACC CCCGGCCTGG GAGCCCCCAC GGCCTTCACC 
AGCGAGGACC TCGTCGTGCA GGTGACCTTA GCCCCAGGTG CGGCTGAGGT CCCTGGGCAG 
CCACGACTGC CAGGGGGAGT CGTGTTCCAC TACCGCCCAG GCTCCTCCCG CTACTCGCTG 
ACCTTTGAGG AGGCCAAGCA GGCCTGCCTG CGCACCGGGG CCATCATCGC CTCGCCCGAG 
CAGCTCCAGG CCGCCTATGA AGCAGGCTAC GAGCAGTGTG ACGCCGGCTG GCTGCAGGAC 
CAGACAGTCA GATACCCCAT TGTGAGCCCG CGGACCCCCT GTGTGGGTGA CAAGGACAGC 
AGCCCGGGGG TCCGGACCTA CGGCGTGCGG CCACCATCAG AAACCTACGA TGTCTACTGC 
TACGTGGACA GACTCGAGGG GGAGGTGTTC TTCGCCACAC GCCTGGAGCA GTTCACCTTC 
TGGGAAGCCC AGGAGTTCTG TGAATCCCAA AACGCCACTC TGGCCACCAC AGGCCAGCTC 
TATGCTGCCT GGAGCCGTGG TCTGGACAAG TGCTACGCCG GCTGGCTGGC TGACGGCAGC 
CTCCGCTACC CCATCGTCAC CCCAAGGCCC GCCTGTGGCG GGGACAAACC GGGCGTGAGA 
ACCGTCTACC TCTACCCCAA CCAGACGGGC CTCCTGGATC CGCTGTCCCG GCACCACGCC 
TTCTGCTTCC GAGGTGTTTC AGCGGCGCCC TCTCCAGAAG AAGAGGAGGG TAGTGCACCC 
ACAGCAGGCC CTGACGTGGA GGAGTGGATG GTGACACAAG TGGGGCCTGG CGTGGCTGCT 
GTCCCCATCG GGGAGGAGAC GACTGCAATC CCAGGCTTCA CCGTTGAGCC AGAAAACAAG 
ACGGAATGGG AACTTGCCTA CACCCCAGCG GGCACTTTGC CACTACCAGG GATCCCTCCT 
ACATGGCCTC CCACTGGAGA AGCAACAGAG GAGCACACAG AAGGCCCTTC TGCAACGGAA 
GTGCCCTCAG CCTCAGAGAA GCCGTTCCCT TCAGAGGAAC CATTCCCCCC AGAGGAACCA 
TTCCCCTCAG AGAAACCATT CCCCCCAGAG GAACTGTTCC CCTCAGAGAA GCCATTCCCC 
TCAGAAAAGC CGTTCCCCTC AGAGGAACCG TTCCCCTCAG AAAAACCATT CCCCCCAGAG 
GAACTGTTCC CCTCAGAGAA GCCAATCCCC TCAGAGGAGC CATTCCCCTC AGAGGAGCCA 
TTCCCCTCAG AGAAGCCATT CCCCCCAGAG GAACCATTCC CCTCAGAGAA ACCAATCCCC 
TCAGAGGAGC CTTTCCCCTC AGAGAAGCCA TTCCCTTCAG AGGAGCCATT CCCCTCAGAG 
GAGCCATCCA CACTTTCAGC CCCAGTGCCC AGCAGGACTG AGCTGCCAAG CTCTGGGGAG 
GTGTCTGGGG TGCCCGAAAT CAGTGGTGAC TTCACAGGCA GTGGAGAAAT TTCAGGACAC 
CTAGACTTCA GTGGGCAGCC CTCAGGGGAA AGTGCAAGTG GACTGCCCTC TGAAGACCTT 
GACTCCAGTG GGCTCACCTC TACAGTGGGC TCAGGCCTGC CTGTGGAAAG TGGACTGCCT 
TCAGGGGAAG AAGAGAGAAT TACATGGACC AGTGCTCCTA AAGTTGACAG GTTGCCCTCT 
GGGGGTGAGG GCCCAGAAGT TTCTGGAGTA GAGGATATCA GTGGACTTCC TTCTGGAGGA 
GAGGTTCATC TAGAGATCTC TGCCTCTGGA GTAGAGGACA TCAGTGGACT TCCTTCTGGA 
GGAGAGGTTC ATCTAGAGAT CTCTGCCTCT GGAGTAGAGG ACCTAAGTAG AATTCCTTCT 
GGAGAAGGTC CAGAAATCTC TGCCTCTGGA GTAGAGGACA TCAGTGGACT TCCTTCTGGA 
GAGGAAGGTC ATCTAGAGAT CTCTGCCTCT GGAGTAGAAG ACCTCAGTGG AATTCCTTCT 
GGAGAAGGTC CAGAAGTCTC TGCTTCTGGA GTAGAGGACC TTATTGGACT TCCTTCTGGA 
GAAGGTCCAG AAGTCTCTGC TTCTGGAGTA GAGGACCTCA GTAGACTTCC TTCTGGAGAA 
GGTCCAGAAG TCTCTGCTTC TGGGGTAGAG GACCTTAGTG GACTTCCTTC TGGAGAAGGT 
CCAGAAGTCT CTGTTTCTGG AGTAGAGGAT CTCAGCAGAC TTCCTTCTGG AGAAGGTCCA 
GAAGTCTCTG CTTCTGGAGT AGAGGACCTC AGTAGACTTC CTTCTGGAGA AGGTCCAGAA 
ATCTCTGTGT CTGGAGTAGA GGACATCAGT ATACTTCCTT CTGGAGAAGG TCCAGAAGTC 
TCTGCCTCTG GAGTAGAGGA CCTCAGTGTT CTTCCTTCTG GAGAAGGTCA TCTAGAGATC 
TCTACCTCTG GAGTAGAGGA CCTCAGTGTT CTTCCTTCTG GTGAAGGTCA TCTAGAGATC 
TCTGCCTCTG GAGTAGAGGA CATCAGTAGA CTTCCTTCTG GAGAAGGTCC AGAAGTCTCT 
GCCTCTGGAG TAGAGGACCT CAGTGTTCTT CCTTCTGGAG AAGATCATCT AGAGATCTCT 
GCCTCTGGAG TAGAGGACCT CGGTGTTCTT CCTTCTGGAG AAGATCATCT AGAGATCTCT 
GCCTCTGGAG TAGAGGACAT CAGTAGACTT CCTTCTGGAG AAGGTCCAGA AGTCTCTGCC 
TCTGGAGTAG AGGACCTCAG TGTTCTTCCT TCTGGAGAAG GTCATCTAGA GATCTCTGCC 
TCTGGAGTAG AGGACCTCAG TAGACTTCCT TCTGGAGGAG AGGATCATCT AGAGACTTCT 
GCTTCTGGAG TAGGGGACCT TAGTGGACTT CCTTCTGGAA GGGAAGGTCT GGAGATCTCT 
GCTTCTGGAG CTGGGGATCT TAGTGGGTTG ACTTCTGGAA AAGAAGACTT GACTGGGTCA 
GCTTCTGGAG CCTTGGACCT TGGCAGAATA CCTTCTGTAA CTCTAGGAAG TGGGCAAGCT 
CCAGAAGCAA GTGGTCTTCC TTCTGGATTT AGTGGTGAGT ATTCTGGGGT GGACCTTGAA 
AGTGGCCCAT CCTCTGGCCT TCCTGATTTC AGTGGACTTC CATCTGGGTT CCCAACTGTC 
TCCCTAGTGG ATACTACATT GGTGGAAGTC GTCACAGCCA CCACAGCAGG TGAACTAGAA 
GGAAGGGGAA CCATTGACAT CAGCGGTGCT GGAGAAACAT CTGGGCTGCC CTTCAGTGAG 
TTGGACATTA GTGGAGGAGC AAGCGGACTC TCTTCAGGAG CTGAACTCAG TGGCCAAGCA 
TCTGGGTCTC CTGACATCAG TGGGGAAACC TCTGGACTCT TTGGTGTCAG TGGACAGCCC 
TCAGGGTTTC CTGACATTAG TGGGGAAACT TCCGGGCTTC TTGAGGTCAG TGGGCAGCCA 
TCAGGGTTTT ATGGAGAAAT ATCTGGCGTG ACTGAGCTTA GCGGACTGGC CTCTGGACAA 
CCAGAAATCA GTGGAGAAGC TTCTGGAATT CTTTCTGGCC TTGGTCCACC ATTCGGCATA 
ACCGACCTGA GCGGAGAAGC ACCCGGGATC CCTGATCTCA GTGGGCAACC ATCGGGCTTG 
CCAGAGTTCA GTGGGACAAC ATCCGGGATC CCTGACCTGG TTTCCAGTGC TGTGAGTGGC 
AGTGGTGAAT CTTCTGGCAT TACGTTCGTG GACACCAGTT TGGTTGAAGT GACCCCAACG 
ACATTTAAAG AAGAAGAAGG CTTAGGATCT GTGGAACTCA GTGGCCTCCC TTCAGGAGAG 
GTGGGTGTCT CAGGCACATC TGGGCTAGCG GATGTCAGTG GACTGTCTTC TGGAGCAATT 
GACTCCAGTG GGTTTACGTC CCAGCCTCCA GAATTCAGTG GCCTGCCAAG CGGAGTAACT 
GAGGTCAGTG GAGAAGCCTC CGGAGCTGAA AGTGGGAGCA GCCTGCCCTC CGGAGCATAT 
GACAGCAGTG GACTTCCGTC TGGTTTCCCC ACTGTCTCTT TTGTAGACAG GACTTTGGTG 
GAATCTGTAA CCCAGGCTCC AACTGCTCAA GAAGCAGGAG AAGGGCCTTC AGGCATTCTG 
GAACTTAGCG GTGCGCCTTC TGGAGCACCA GACATGTCTG GAGACCATTT GGGATCTTTG 
GACCAAAGTG GGCTTCAGTC TGGACTAGTG GAGCCCAGTG GGGAGCCCGC AAGTACTCCA 
TATTTTAGTG GGGACTTTTC TGGCACCACT GATGTAAGTG GGGAATCCTC TGCAGCCACG 
AGCACCAGTG GGGAGGCCTC CGGACTTCCA GAAGTTACGT TAATCACATC TGAGTTGGTG 
GAGGGAGTTA CTGAACCAAC CGTTTCCCAG GAACTGGGCC AGAGACCCCC CGTAACATAC 
ACCCCCCAGC TTTTTGAATC CAGTGGTGAA GCCTCTGCAT CTGGGGATGT GCCAAGGTTC 
CCTGGATCGG GGGTAGAAGT GTCATCAGTC CCAGAATCCA GCGGTGAGAC GTCAGCCTAT 
CCCGAGGCTG AGGTGGGAGC ATCTGCTGCC CCCGAGGCAA GCGGAGGAGC TTCCGGGTCC 
CCTAACCTGA GTGAAACCAC CTCCACCTTC CATGAAGCTG ATCTGGAGGG AACCTCAGGC 
CTGGGAGTGA GTGGCAGCCC CTCAGCCTTT CCAGAAGGCC CCACGGAGGG CTTGGCCACC 
CCGGAAGTGA GTGGAGAGTC AACCACTGCC TTTGACGTGA GCGTAGAGGC ATCAGGCTCA 
CCTTCTGCCA CTCCCCTGGC TTCTGGAGAC AGGACTGACA CCAGCGGAGA TCTGTCTGGC 
CACACCTCGG GGCTGGATAT TGTCATCAGC ACCACCATCC CAGAATCCGA GTGGACTCAG 
CAGACCCAGC GCCCTGCAGA GGCGCGTCTA GAAATCGAAT CCTCAAGCCC TGTGCACTCA 
GGAGAAGAGA GCCAAACAGC CGACACAGCC ACCTCCCCGA CTGATGCTTC TATCCCAGCC 
TCCGCAGGAG GGACAGATGA TTCAGAGGCA ACCACAACAG ACATTGACGA GTGCCTCTCA 
AGCCCTTGTC TGAATGGAGC CACCTGCGTG GATGCCATCG ACTCTTTCAC ATGCTTATGC 
CTTCCCAGCT ACCAAGGGGA CGTGTGTGAG ATTGACCAGA AGCTGTGCGA GGAGGGCTGG 
ACCAAGTTCC AGGGCCACTG TTACCGCCAC TTCCCCGACC GGGCAACCTG GGTGGACGCT 
GAGAGCCAGT GCCGGAAGCA GCAGTCACAC CTGAGCAGCA TCGTCACCCC CGAGGAGCAG 
GAGTTTGTCA ACAACAATGC CCAGGACTAC CAGTGGATCG GCCTGAACGA CAAGACCATC 
GAAGGGGACT TCCGCTGGTC AGATGGACAC TCCTTGCAAT TTGAGAACTG GCGCCCCAAC 
CAGCCTGACA ACTTCTTTGC CACTGGGGAG GACTGTGTGG TGATGATCTG GCATGAGAAG 
GGCGAGTGGA ACGATGTCCC ATGTAATTAC CAGCTACCCT TCACCTGTAA AAAGGGCACA 
GTGGCCTGCG GAGAGCCCCC CGTGGTGGAG CATGCCAGAA TCTTCGGACA GAAGAAGGAC 
CGATACGAGA TCAATGCCCT GGTGCGGTAC CAGTGCACCG AGGGCTTCAT CCAGCGCCAT 
GTGCCCACCA TCCGGTGCCA GCCCAGCGGG CACTGGGAGG AGCCTCGGAT CACCTGCACA 
GACCCCGCTA CCTACAAGCG CAGACTACAG AAGCGGAGCT CACGGCCCCT GAGGAGGAGC 
CACCCCAGCA CGGCCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Antigen-specific B lymphocytes acquire proteoglycan aggrecan from cartilage extracellular matrix resulting in antigen presentation and CD4+ T-cell activation.
Immunology141(1)70-8(2014 Jan)
Ciechomska M,Wilson CL,Floudas A,Hui W,Rowan AD,van Eden W,Robinson JH,Knight AM

Microvesicles shed by oligodendroglioma cells and rheumatoid synovial fibroblasts contain aggrecanase activity.
Matrix biology : journal of the International Society for Matrix Biology31(4)229-33(2012 May)
Lo Cicero A,Majkowska I,Nagase H,Di Liegro I,Troeberg L

Disc strain and resulting positive mRNA expression from application of a noninvasive treatment.
Spine36(14)E921-8(2011 Jun)
Desmoulin GT,Hewitt CR,Hunter CJ

MMPs are less efficient than ADAMTS5 in cleaving aggrecan core protein.
Matrix biology : journal of the International Society for Matrix Biology30(2)145-53(2011 Mar)
Durigova M,Nagase H,Mort JS,Roughley PJ

Single-molecule force spectroscopy of cartilage aggrecan self-adhesion.
Biophysical journal99(10)3498-504(2010 Nov)
Harder A,Walhorn V,Dierks T,Fern?ndez-Busquets X,Anselmetti D

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ACAN cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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