RAPGEF4 cDNA ORF clone, Equus caballus(horse)

The following RAPGEF4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAPGEF4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHb36340 XM_014732537.1
Latest version!
Equus caballus Rap guanine nucleotide exchange factor 4 (RAPGEF4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OHb52130 XM_023622000.1
Latest version!
Equus caballus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHb52131 XM_023622001.1
Latest version!
Equus caballus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHb52132 XM_023622002.1
Latest version!
Equus caballus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHb52133 XM_023622003.1
Latest version!
Equus caballus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHb52134 XM_023622006.1
Latest version!
Equus caballus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHb52134 XM_023622004.1
Latest version!
Equus caballus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHb52134 XM_023622005.1
Latest version!
Equus caballus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHb52127 XM_023621997.1
Latest version!
Equus caballus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHb52128 XM_023621998.1
Latest version!
Equus caballus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHb52129 XM_023621999.1
Latest version!
Equus caballus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHb36340
    Clone ID Related Accession (Same CDS sequence) XM_014732537.1
    Accession Version XM_014732537.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product LOW QUALITY PROTEIN: rap guanine nucleotide exchange factor 4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867384.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGGAC TTCTGGCTCC TCCTTATGGT GTTATGGAAA CGGGCTCTAA CAATGACAGG 
    ATTCCTGACA AGGAGAATAC ACCTCTCATT GAACCTCACG TTCCTCTTCG TCCTGCTAAC
    ACCATTACCA AGGTCCCTTC AGAGAAGATC CTCAGGGCTG GAAAAATTTT ACGAAACGCC
    ATTCTCTCTC GAGCACCTCA CATGATTAGA GATAGGAAAT ACCACCTAAA GACGTACAGA
    CAATGCTGTG TGGGAACTGA GCTGGTGGAC TGGATGCTGC AGCAGACTCC GTGTGCCCAC
    TCCCGGACTC AAGCTGTTGG GATGTGGCAA GTGCTGGTGG AAGATGGTGT TCTCAACCAT
    GTGGACCAGG AGCATCATTT CCAAGACAAA TATTTATTTT ATCGATTTCT GGATGACGAG
    CACGAGGATG CCCCTTTGCC TACTGAGGAG GAGAAGAAGG AGTGTGATGA GGAGCTCCAG
    GACACCATGC TGCTGCTGTC ACAGATGGGC CCCGATGCCC ACATGAGGAT GATCCTCCGC
    AAACCACCTG GCCAGAGGAC TGTGGATGAC CTAGAAATTA TCTACGAAGA GCTCCTTCAC
    ATTAAAGCCT TATCCCACCT TTCCACCACT GTGAAACGGG AGTTGGCAGG TGTTCTCATT
    TTTGAGTCGC ACGCCAAAGG AGGAACCGTG TTGTTCAACC AGGGGGAAGA AGGTACCTCC
    TGGTACATTA TTCTAAAAGG ATCGGTGAAT GTAGTCATTT ATGGCAAGGG TGTGGTCTGC
    ACCCTGCATG AGGGAGATGA CTTTGGCAAG CTAGCACTAG TGAACGACGC CCCGAGAGCT
    GCCTCCATCG TCTTGCGAGA AGATAATTGC CATTTCTTAA GAGTGGACAA GGAGGATTTC
    AACCGAATCC TGAGGGACGT TGAGGCGAAT ACAGTCAGAC TTAAAGAACA TGACCAAGAT
    GTCTTGGTGC TGGAGAAGGT CCCAGCAGGG AACAGGGCTT CTAATCAAGG AAACTCACAG
    CCTCAGCAAA AGTATACTGT GATGTCAGGA ACACCTGAAA AAATTTTAGA GCATTTTCTA
    GAAACAATAC GCCTTGAGCC AGCTTTAAAT GAAGCAACAG ATTCTGTTTT AAACGACTTT
    GTTATGATGC ACTGTGTTTT TATGCCAAAT ATCCAGCTTT GTCCAGCCCT GGTGGCCCAT
    TACCATGCAC AGCCCTCACA AGGTACAGAA CAGGAGAAAA TGGATTATGC CCTCAACAAT
    AGGAGACGAG TCATCCGGCT GGTTTTACAT TGGGCCGCCA TGTATGGAGA CGTCCTACAA
    GAGGATGACG TAGCTATGGC TTTCCTGGAG GAGTTTTATG TGTCTGTGTC AGATGATGCC
    CGGATGATTG CTGCCCTCAA GGAGCAACTG CCCGAGCTGG AGAAGATCGT CAAGCAAATT
    TCAGAAGATG CAAAAGCTCC ACAAAAGAAG CACAAAGTTC TTTTGCAACA GTTCAACACA
    GGCGATGAGA GAGCCCAGAA GCGCCAGCCT ATCCGTGGCT CTGATGAAGT TCTGTTTAAG
    GTCTATTGCA TGGACCACAC CTACACAACC ATTCGGGTGC CGGTGGCCGC CTCAGTGAAG
    GAAGTCATCA GCGCAGTTGC CGACAAACTG GGCTCCGGAG AAGGCCTGAT CATAGTCAAG
    ATGAGTTCCG GAGGAGAAAA GGTGGTGCTC AAACCTAATG ATGTTTCAGT ATTTACAACG
    CTCACCATTA ATGGACGCCT GTTTGCTTGC CCGCGAGAGC AATTCGACTC ACTGACTCCC
    CTGCCAGAAC AGGAAGGCCC AACTGTTGGA ACAGTGGGAA CTTTTGAACT GATGAGCTCC
    AAAGATTTAG CATACCAGAT GACAATTTAT GATTGGGAAC TCTTCAACTG TGTGCATGAG
    CTGGAGCTAA TCTATCACAC ATTTGGAAGG CATAATTTTA AAAAGACCAC AGCAAACTTG
    GATTTGTTCC TGAGAAGATT TAATGAAATT CAGTTTTGGG TTGTCACCGA GATTTGCCTT
    TGTCCCCAGC CCAGCAAGCG TGTTCAGCTC TTAAAAAAAT TTATTAAGAT CGCAGCCCAC
    TGTAAGGAGT ACAAAAATCT GAATTCCTTT TTTGCCATCG TCATGGGACT AAGTAATGTT
    GCCGTGAGCC GTTTGGCACT AACGTGGGAG AAACTGCCAA GCAAGTTCAA GAAGTTCTAT
    GCGGAGTTTG AAAGCTTAAT GGATCCTTCG AGAAACCACA GGGCCTACCG GCTGACCATA
    GCTAAGCTGG ACCCCCCTCT CATCCCCTTC ATGCCTTTGC TCATTAAAGA TATGACATTT
    ACTCATGAGG GGAACAAGAC ATTCATTGAC AATCTAGTAA ACTTTGAAAA AATGCGCATG
    ATTGCAAATA CTGCCAGAAC AGTGAGATAC TGCAGGAGCC AACCCTTCAA TCCTGATGCC
    GCTCAAGCTA ATAAGAACCA TCAGGATGTC CGGAGTTATG TACGGCAATT AAATGTGATT
    GACAACCAGA GAACTTTATC ACAGATGTCA CACAGATTAG AGCCTCGTCG ACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014588023.1
    CDS391..2967
    Translation

    Target ORF information:

    RefSeq Version XM_014732537.1
    Organism Equus caballus(horse)
    Definition Equus caballus Rap guanine nucleotide exchange factor 4 (RAPGEF4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014732537.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGGAC TTCTGGCTCC TCCTTATGGT GTTATGGAAA CGGGCTCTAA CAATGACAGG 
    ATTCCTGACA AGGAGAATAC ACCTCTCATT GAACCTCACG TTCCTCTTCG TCCTGCTAAC
    ACCATTACCA AGGTCCCTTC AGAGAAGATC CTCAGGGCTG GAAAAATTTT ACGAAACGCC
    ATTCTCTCTC GAGCACCTCA CATGATTAGA GATAGGAAAT ACCACCTAAA GACGTACAGA
    CAATGCTGTG TGGGAACTGA GCTGGTGGAC TGGATGCTGC AGCAGACTCC GTGTGCCCAC
    TCCCGGACTC AAGCTGTTGG GATGTGGCAA GTGCTGGTGG AAGATGGTGT TCTCAACCAT
    GTGGACCAGG AGCATCATTT CCAAGACAAA TATTTATTTT ATCGATTTCT GGATGACGAG
    CACGAGGATG CCCCTTTGCC TACTGAGGAG GAGAAGAAGG AGTGTGATGA GGAGCTCCAG
    GACACCATGC TGCTGCTGTC ACAGATGGGC CCCGATGCCC ACATGAGGAT GATCCTCCGC
    AAACCACCTG GCCAGAGGAC TGTGGATGAC CTAGAAATTA TCTACGAAGA GCTCCTTCAC
    ATTAAAGCCT TATCCCACCT TTCCACCACT GTGAAACGGG AGTTGGCAGG TGTTCTCATT
    TTTGAGTCGC ACGCCAAAGG AGGAACCGTG TTGTTCAACC AGGGGGAAGA AGGTACCTCC
    TGGTACATTA TTCTAAAAGG ATCGGTGAAT GTAGTCATTT ATGGCAAGGG TGTGGTCTGC
    ACCCTGCATG AGGGAGATGA CTTTGGCAAG CTAGCACTAG TGAACGACGC CCCGAGAGCT
    GCCTCCATCG TCTTGCGAGA AGATAATTGC CATTTCTTAA GAGTGGACAA GGAGGATTTC
    AACCGAATCC TGAGGGACGT TGAGGCGAAT ACAGTCAGAC TTAAAGAACA TGACCAAGAT
    GTCTTGGTGC TGGAGAAGGT CCCAGCAGGG AACAGGGCTT CTAATCAAGG AAACTCACAG
    CCTCAGCAAA AGTATACTGT GATGTCAGGA ACACCTGAAA AAATTTTAGA GCATTTTCTA
    GAAACAATAC GCCTTGAGCC AGCTTTAAAT GAAGCAACAG ATTCTGTTTT AAACGACTTT
    GTTATGATGC ACTGTGTTTT TATGCCAAAT ATCCAGCTTT GTCCAGCCCT GGTGGCCCAT
    TACCATGCAC AGCCCTCACA AGGTACAGAA CAGGAGAAAA TGGATTATGC CCTCAACAAT
    AGGAGACGAG TCATCCGGCT GGTTTTACAT TGGGCCGCCA TGTATGGAGA CGTCCTACAA
    GAGGATGACG TAGCTATGGC TTTCCTGGAG GAGTTTTATG TGTCTGTGTC AGATGATGCC
    CGGATGATTG CTGCCCTCAA GGAGCAACTG CCCGAGCTGG AGAAGATCGT CAAGCAAATT
    TCAGAAGATG CAAAAGCTCC ACAAAAGAAG CACAAAGTTC TTTTGCAACA GTTCAACACA
    GGCGATGAGA GAGCCCAGAA GCGCCAGCCT ATCCGTGGCT CTGATGAAGT TCTGTTTAAG
    GTCTATTGCA TGGACCACAC CTACACAACC ATTCGGGTGC CGGTGGCCGC CTCAGTGAAG
    GAAGTCATCA GCGCAGTTGC CGACAAACTG GGCTCCGGAG AAGGCCTGAT CATAGTCAAG
    ATGAGTTCCG GAGGAGAAAA GGTGGTGCTC AAACCTAATG ATGTTTCAGT ATTTACAACG
    CTCACCATTA ATGGACGCCT GTTTGCTTGC CCGCGAGAGC AATTCGACTC ACTGACTCCC
    CTGCCAGAAC AGGAAGGCCC AACTGTTGGA ACAGTGGGAA CTTTTGAACT GATGAGCTCC
    AAAGATTTAG CATACCAGAT GACAATTTAT GATTGGGAAC TCTTCAACTG TGTGCATGAG
    CTGGAGCTAA TCTATCACAC ATTTGGAAGG CATAATTTTA AAAAGACCAC AGCAAACTTG
    GATTTGTTCC TGAGAAGATT TAATGAAATT CAGTTTTGGG TTGTCACCGA GATTTGCCTT
    TGTCCCCAGC CCAGCAAGCG TGTTCAGCTC TTAAAAAAAT TTATTAAGAT CGCAGCCCAC
    TGTAAGGAGT ACAAAAATCT GAATTCCTTT TTTGCCATCG TCATGGGACT AAGTAATGTT
    GCCGTGAGCC GTTTGGCACT AACGTGGGAG AAACTGCCAA GCAAGTTCAA GAAGTTCTAT
    GCGGAGTTTG AAAGCTTAAT GGATCCTTCG AGAAACCACA GGGCCTACCG GCTGACCATA
    GCTAAGCTGG ACCCCCCTCT CATCCCCTTC ATGCCTTTGC TCATTAAAGA TATGACATTT
    ACTCATGAGG GGAACAAGAC ATTCATTGAC AATCTAGTAA ACTTTGAAAA AATGCGCATG
    ATTGCAAATA CTGCCAGAAC AGTGAGATAC TGCAGGAGCC AACCCTTCAA TCCTGATGCC
    GCTCAAGCTA ATAAGAACCA TCAGGATGTC CGGAGTTATG TACGGCAATT AAATGTGATT
    GACAACCAGA GAACTTTATC ACAGATGTCA CACAGATTAG AGCCTCGTCG ACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb52130
    Clone ID Related Accession (Same CDS sequence) XM_023622000.1
    Accession Version XM_023622000.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2982bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product rap guanine nucleotide exchange factor 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCGTCGGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCGC TGGAACGATC CAGTGAAGAT GTAGATATAA TCTTCACACG GCTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGTTT GTGTGGTTAT
    TATGAGAATC TGGAAAAAGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AAACCAGCAG TCACCAGGAT
    GCAGTGACCA TCTGTACTCT GGGAATTGGG ACAGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCGACCAT CGTTACCAGG GAGAGCAGCG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTACA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAATGTC
    CCTTCAGAGA AGATCCTCAG GGCTGGAAAA ATTTTACGAA ACGCCATTCT CTCTCGAGCA
    CCTCACATGA TTAGAGATAG GAAATACCAC CTAAAGACGT ACAGACAATG CTGTGTGGGA
    ACTGAGCTGG TGGACTGGAT GCTGCAGCAG ACTCCGTGTG CCCACTCCCG GACTCAAGCT
    GTTGGGATGT GGCAAGTGCT GGTGGAAGAT GGTGTTCTCA ACCATGTGGA CCAGGAGCAT
    CATTTCCAAG ACAAATATTT ATTTTATCGA TTTCTGGATG ACGAGCACGA GGATGCCCCT
    TTGCCTACTG AGGAGGAGAA GAAGGAGTGT GATGAGGAGC TCCAGGACAC CATGCTGCTG
    CTGTCACAGA TGGGCCCCGA TGCCCACATG AGGATGATCC TCCGCAAACC ACCTGGCCAG
    AGGACTGTGG ATGACCTAGA AATTATCTAC GAAGAGCTCC TTCACATTAA AGCCTTATCC
    CACCTTTCCA CCACTGTGAA ACGGGAGTTG GCAGGTGTTC TCATTTTTGA GTCGCACGCC
    AAAGGAGGAA CCGTGTTGTT CAACCAGGGG GAAGAAGGTA CCTCCTGGTA CATTATTCTA
    AAAGGATCGG TGAATGTAGT CATTTATGGC AAGGGTGTGG TCTGCACCCT GCATGAGGGA
    GATGACTTTG GCAAGCTAGC ACTAGTGAAC GACGCCCCGA GAGCTGCCTC CATCGTCTTG
    CGAGAAGATA ATTGCCATTT CTTAAGAGTG GACAAGGAGG ATTTCAACCG AATCCTGAGG
    GACGTTGAGG CGAATACAGT CAGACTTAAA GAACATGACC AAGATGTCTT GGTGCTGGAG
    AAGGTCCCAG CAGGGAACAG GGCTTCTAAT CAAGGAAACT CACAGCCTCA GCAAAAGTAT
    ACTGTGATGT CAGGAACACC TGAAAAAATT TTAGAGCATT TTCTAGAAAC AATACGCCTT
    GAGCCAGCTT TAAATGAAGC AACAGATTCT GTTTTAAACG ACTTTGTTAT GATGCACTGT
    GTTTTTATGC CAAATATCCA GCTTTGTCCA GCCCTGGTGG CCCATTACCA TGCACAGCCC
    TCACAAGGTA CAGAACAGGA GAAAATGGAT TATGCCCTCA ACAATAAGAG ACGAGTCATC
    CGGCTGGTTT TACAGTGGGC CGCCATGTAT GGAGACGTCC TACAAGAGGA TGACGTAGCT
    ATGGCTTTCC TGGAGGAGTT TTATGTGTCT GTGTCAGATG ATGCCCGGAT GATTGCTGCC
    CTCAAGGAGC AACTGCCCGA GCTGGAGAAG ATCGTCAAGC AAATTTCAGA AGATGCAAAA
    GCTCCACAAA AGAAGCACAA AGTTCTTTTG CAACAGTTCA ACACAGGCGA TGAGAGAGCC
    CAGAAGCGCC AGCCTATCCG TGGCTCTGAT GAAGTTCTGT TTAAGGTCTA TTGCATGGAC
    CACACCTACA CAACCATTCG GGTGCCGGTG GCCGCCTCAG TGAAGGAAGT CATCAGCGCA
    GTTGCCGACA AACTGGGCTC CGGAGAAGGC CTGATCATAG TCAAGATGAG TTCCGGAGGA
    GAAAAGGTGG TGCTCAAACC TAATGATGTT TCAGTATTTA CAACGCTCAC CATTAATGGA
    CGCCTGTTTG CTTGCCCGCG AGAGCAATTC GACTCACTGA CTCCCCTGCC AGAACAGGAA
    GGCCCAACTG TTGGAACAGT GGGAACTTTT GAACTGATGA GCTCCAAAGA TTTAGCATAC
    CAGATGACAA TTTATGATTG GGAACTCTTC AACTGTGTGC ATGAGCTGGA GCTAATCTAT
    CACACATTTG GAAGGCATAA TTTTAAAAAG ACCACAGCAA ACTTGGATTT GTTCCTGAGA
    AGATTTAATG AAATTCAGTT TTGGGTTGTC ACCGAGATTT GCCTTTGTCC CCAGCCCAGC
    AAGCGTGTTC AGCTCTTAAA AAAATTTATT AAGATCGCAG CCCACTGTAA GGAGTACAAA
    AATCTGAATT CCTTTTTTGC CATCGTCATG GGACTAAGTA ATGTTGCCGT GAGCCGTTTG
    GCACTAACGT GGGAGAAACT GCCAAGCAAG TTCAAGAAGT TCTATGCGGA GTTTGAAAGC
    TTAATGGATC CTTCGAGAAA CCACAGGGCC TACCGGCTGA CCATAGCTAA GCTGGACCCC
    CCTCTCATCC CCTTCATGCC TTTGCTCATT AAAGATATGA CATTTACTCA TGAGGGGAAC
    AAGACATTCA TTGACAATCT AGTAAACTTT GAAAAAATGC GCATGATTGC AAATACTGCC
    AGAACAGTGA GATACTGCAG GAGCCAACCC TTCAATCCTG ATGCCGCTCA AGCTAATAAG
    AACCATCAGG ATGTCCGGAG TTATGTACGG CAATTAAATG TGATTGACAA CCAGAGAACT
    TTATCACAGA TGTCACACAG ATTAGAGCCT CGTCGACCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023477768.1
    CDS111..3092
    Translation

    Target ORF information:

    RefSeq Version XM_023622000.1
    Organism Equus caballus(horse)
    Definition Equus caballus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023622000.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCGTCGGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCGC TGGAACGATC CAGTGAAGAT GTAGATATAA TCTTCACACG GCTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGTTT GTGTGGTTAT
    TATGAGAATC TGGAAAAAGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AAACCAGCAG TCACCAGGAT
    GCAGTGACCA TCTGTACTCT GGGAATTGGG ACAGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCGACCAT CGTTACCAGG GAGAGCAGCG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTACA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAATGTC
    CCTTCAGAGA AGATCCTCAG GGCTGGAAAA ATTTTACGAA ACGCCATTCT CTCTCGAGCA
    CCTCACATGA TTAGAGATAG GAAATACCAC CTAAAGACGT ACAGACAATG CTGTGTGGGA
    ACTGAGCTGG TGGACTGGAT GCTGCAGCAG ACTCCGTGTG CCCACTCCCG GACTCAAGCT
    GTTGGGATGT GGCAAGTGCT GGTGGAAGAT GGTGTTCTCA ACCATGTGGA CCAGGAGCAT
    CATTTCCAAG ACAAATATTT ATTTTATCGA TTTCTGGATG ACGAGCACGA GGATGCCCCT
    TTGCCTACTG AGGAGGAGAA GAAGGAGTGT GATGAGGAGC TCCAGGACAC CATGCTGCTG
    CTGTCACAGA TGGGCCCCGA TGCCCACATG AGGATGATCC TCCGCAAACC ACCTGGCCAG
    AGGACTGTGG ATGACCTAGA AATTATCTAC GAAGAGCTCC TTCACATTAA AGCCTTATCC
    CACCTTTCCA CCACTGTGAA ACGGGAGTTG GCAGGTGTTC TCATTTTTGA GTCGCACGCC
    AAAGGAGGAA CCGTGTTGTT CAACCAGGGG GAAGAAGGTA CCTCCTGGTA CATTATTCTA
    AAAGGATCGG TGAATGTAGT CATTTATGGC AAGGGTGTGG TCTGCACCCT GCATGAGGGA
    GATGACTTTG GCAAGCTAGC ACTAGTGAAC GACGCCCCGA GAGCTGCCTC CATCGTCTTG
    CGAGAAGATA ATTGCCATTT CTTAAGAGTG GACAAGGAGG ATTTCAACCG AATCCTGAGG
    GACGTTGAGG CGAATACAGT CAGACTTAAA GAACATGACC AAGATGTCTT GGTGCTGGAG
    AAGGTCCCAG CAGGGAACAG GGCTTCTAAT CAAGGAAACT CACAGCCTCA GCAAAAGTAT
    ACTGTGATGT CAGGAACACC TGAAAAAATT TTAGAGCATT TTCTAGAAAC AATACGCCTT
    GAGCCAGCTT TAAATGAAGC AACAGATTCT GTTTTAAACG ACTTTGTTAT GATGCACTGT
    GTTTTTATGC CAAATATCCA GCTTTGTCCA GCCCTGGTGG CCCATTACCA TGCACAGCCC
    TCACAAGGTA CAGAACAGGA GAAAATGGAT TATGCCCTCA ACAATAAGAG ACGAGTCATC
    CGGCTGGTTT TACAGTGGGC CGCCATGTAT GGAGACGTCC TACAAGAGGA TGACGTAGCT
    ATGGCTTTCC TGGAGGAGTT TTATGTGTCT GTGTCAGATG ATGCCCGGAT GATTGCTGCC
    CTCAAGGAGC AACTGCCCGA GCTGGAGAAG ATCGTCAAGC AAATTTCAGA AGATGCAAAA
    GCTCCACAAA AGAAGCACAA AGTTCTTTTG CAACAGTTCA ACACAGGCGA TGAGAGAGCC
    CAGAAGCGCC AGCCTATCCG TGGCTCTGAT GAAGTTCTGT TTAAGGTCTA TTGCATGGAC
    CACACCTACA CAACCATTCG GGTGCCGGTG GCCGCCTCAG TGAAGGAAGT CATCAGCGCA
    GTTGCCGACA AACTGGGCTC CGGAGAAGGC CTGATCATAG TCAAGATGAG TTCCGGAGGA
    GAAAAGGTGG TGCTCAAACC TAATGATGTT TCAGTATTTA CAACGCTCAC CATTAATGGA
    CGCCTGTTTG CTTGCCCGCG AGAGCAATTC GACTCACTGA CTCCCCTGCC AGAACAGGAA
    GGCCCAACTG TTGGAACAGT GGGAACTTTT GAACTGATGA GCTCCAAAGA TTTAGCATAC
    CAGATGACAA TTTATGATTG GGAACTCTTC AACTGTGTGC ATGAGCTGGA GCTAATCTAT
    CACACATTTG GAAGGCATAA TTTTAAAAAG ACCACAGCAA ACTTGGATTT GTTCCTGAGA
    AGATTTAATG AAATTCAGTT TTGGGTTGTC ACCGAGATTT GCCTTTGTCC CCAGCCCAGC
    AAGCGTGTTC AGCTCTTAAA AAAATTTATT AAGATCGCAG CCCACTGTAA GGAGTACAAA
    AATCTGAATT CCTTTTTTGC CATCGTCATG GGACTAAGTA ATGTTGCCGT GAGCCGTTTG
    GCACTAACGT GGGAGAAACT GCCAAGCAAG TTCAAGAAGT TCTATGCGGA GTTTGAAAGC
    TTAATGGATC CTTCGAGAAA CCACAGGGCC TACCGGCTGA CCATAGCTAA GCTGGACCCC
    CCTCTCATCC CCTTCATGCC TTTGCTCATT AAAGATATGA CATTTACTCA TGAGGGGAAC
    AAGACATTCA TTGACAATCT AGTAAACTTT GAAAAAATGC GCATGATTGC AAATACTGCC
    AGAACAGTGA GATACTGCAG GAGCCAACCC TTCAATCCTG ATGCCGCTCA AGCTAATAAG
    AACCATCAGG ATGTCCGGAG TTATGTACGG CAATTAAATG TGATTGACAA CCAGAGAACT
    TTATCACAGA TGTCACACAG ATTAGAGCCT CGTCGACCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb52131
    Clone ID Related Accession (Same CDS sequence) XM_023622001.1
    Accession Version XM_023622001.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2913bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product rap guanine nucleotide exchange factor 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTGCTTCT CTCTGCTGAT ACTCGTGGGC GTGTATCCTG TGCCTCAGGT GGGTTGGCAT 
    GGATGGAAGA TTGAGACTCA CCGTTCTAGA CCGCTGGAAC GATCCAGTGA AGATGTAGAT
    ATAATCTTCA CACGGCTGAA AGAAGTTAAA GCTTTTGAGA AATTTCACCC AAATCTCCTT
    CATCAGATTT GTTTGTGTGG TTATTATGAG AATCTGGAAA AAGGAATAAC ATTATTTCGC
    CAGGGTGATA TTGGAACAAA CTGGTATGCT GTCCTGGCAG GGTCTTTGGA TGTTAAAGTA
    TCTGAAACCA GCAGTCACCA GAAATATCGA CAGTACATGG CAGGACTTCT GGCTCCTCCT
    TATGGTGTTA TGGAAACGGG CTCTAACAAT GACAGGATTC CTGACAAGGA GAATACACCT
    CTCATTGAAC CTCACGTTCC TCTTCGTCCT GCTAACACCA TTACCAAGGT CCCTTCAGAG
    AAGATCCTCA GGGCTGGAAA AATTTTACGA AACGCCATTC TCTCTCGAGC ACCTCACATG
    ATTAGAGATA GGAAATACCA CCTAAAGACG TACAGACAAT GCTGTGTGGG AACTGAGCTG
    GTGGACTGGA TGCTGCAGCA GACTCCGTGT GCCCACTCCC GGACTCAAGC TGTTGGGATG
    TGGCAAGTGC TGGTGGAAGA TGGTGTTCTC AACCATGTGG ACCAGGAGCA TCATTTCCAA
    GACAAATATT TATTTTATCG ATTTCTGGAT GACGAGCACG AGGATGCCCC TTTGCCTACT
    GAGGAGGAGA AGAAGGAGTG TGATGAGGAG CTCCAGGACA CCATGCTGCT GCTGTCACAG
    ATGGGCCCCG ATGCCCACAT GAGGATGATC CTCCGCAAAC CACCTGGCCA GAGGACTGTG
    GATGACCTAG AAATTATCTA CGAAGAGCTC CTTCACATTA AAGCCTTATC CCACCTTTCC
    ACCACTGTGA AACGGGAGTT GGCAGGTGTT CTCATTTTTG AGTCGCACGC CAAAGGAGGA
    ACCGTGTTGT TCAACCAGGG GGAAGAAGGT ACCTCCTGGT ACATTATTCT AAAAGGATCG
    GTGAATGTAG TCATTTATGG CAAGGGTGTG GTCTGCACCC TGCATGAGGG AGATGACTTT
    GGCAAGCTAG CACTAGTGAA CGACGCCCCG AGAGCTGCCT CCATCGTCTT GCGAGAAGAT
    AATTGCCATT TCTTAAGAGT GGACAAGGAG GATTTCAACC GAATCCTGAG GGACGTTGAG
    GCGAATACAG TCAGACTTAA AGAACATGAC CAAGATGTCT TGGTGCTGGA GAAGGTCCCA
    GCAGGGAACA GGGCTTCTAA TCAAGGAAAC TCACAGCCTC AGCAAAAGTA TACTGTGATG
    TCAGGAACAC CTGAAAAAAT TTTAGAGCAT TTTCTAGAAA CAATACGCCT TGAGCCAGCT
    TTAAATGAAG CAACAGATTC TGTTTTAAAC GACTTTGTTA TGATGCACTG TGTTTTTATG
    CCAAATATCC AGCTTTGTCC AGCCCTGGTG GCCCATTACC ATGCACAGCC CTCACAAGGT
    ACAGAACAGG AGAAAATGGA TTATGCCCTC AACAATAAGA GACGAGTCAT CCGGCTGGTT
    TTACAGTGGG CCGCCATGTA TGGAGACGTC CTACAAGAGG ATGACGTAGC TATGGCTTTC
    CTGGAGGAGT TTTATGTGTC TGTGTCAGAT GATGCCCGGA TGATTGCTGC CCTCAAGGAG
    CAACTGCCCG AGCTGGAGAA GATCGTCAAG CAAATTTCAG AAGATGCAAA AGCTCCACAA
    AAGAAGCACA AAGTTCTTTT GCAACAGTTC AACACAGGCG ATGAGAGAGC CCAGAAGCGC
    CAGCCTATCC GTGGCTCTGA TGAAGTTCTG TTTAAGGTCT ATTGCATGGA CCACACCTAC
    ACAACCATTC GGGTGCCGGT GGCCGCCTCA GTGAAGGAAG TCATCAGCGC AGTTGCCGAC
    AAACTGGGCT CCGGAGAAGG CCTGATCATA GTCAAGATGA GTTCCGGAGG AGAAAAGGTG
    GTGCTCAAAC CTAATGATGT TTCAGTATTT ACAACGCTCA CCATTAATGG ACGCCTGTTT
    GCTTGCCCGC GAGAGCAATT CGACTCACTG ACTCCCCTGC CAGAACAGGA AGGCCCAACT
    GTTGGAACAG TGGGAACTTT TGAACTGATG AGCTCCAAAG ATTTAGCATA CCAGATGACA
    ATTTATGATT GGGAACTCTT CAACTGTGTG CATGAGCTGG AGCTAATCTA TCACACATTT
    GGAAGGCATA ATTTTAAAAA GACCACAGCA AACTTGGATT TGTTCCTGAG AAGATTTAAT
    GAAATTCAGT TTTGGGTTGT CACCGAGATT TGCCTTTGTC CCCAGCCCAG CAAGCGTGTT
    CAGCTCTTAA AAAAATTTAT TAAGATCGCA GCCCACTGTA AGGAGTACAA AAATCTGAAT
    TCCTTTTTTG CCATCGTCAT GGGACTAAGT AATGTTGCCG TGAGCCGTTT GGCACTAACG
    TGGGAGAAAC TGCCAAGCAA GTTCAAGAAG TTCTATGCGG AGTTTGAAAG CTTAATGGAT
    CCTTCGAGAA ACCACAGGGC CTACCGGCTG ACCATAGCTA AGCTGGACCC CCCTCTCATC
    CCCTTCATGC CTTTGCTCAT TAAAGATATG ACATTTACTC ATGAGGGGAA CAAGACATTC
    ATTGACAATC TAGTAAACTT TGAAAAAATG CGCATGATTG CAAATACTGC CAGAACAGTG
    AGATACTGCA GGAGCCAACC CTTCAATCCT GATGCCGCTC AAGCTAATAA GAACCATCAG
    GATGTCCGGA GTTATGTACG GCAATTAAAT GTGATTGACA ACCAGAGAAC TTTATCACAG
    ATGTCACACA GATTAGAGCC TCGTCGACCA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023477769.1
    CDS97..3009
    Translation

    Target ORF information:

    RefSeq Version XM_023622001.1
    Organism Equus caballus(horse)
    Definition Equus caballus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023622001.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTGCTTCT CTCTGCTGAT ACTCGTGGGC GTGTATCCTG TGCCTCAGGT GGGTTGGCAT 
    GGATGGAAGA TTGAGACTCA CCGTTCTAGA CCGCTGGAAC GATCCAGTGA AGATGTAGAT
    ATAATCTTCA CACGGCTGAA AGAAGTTAAA GCTTTTGAGA AATTTCACCC AAATCTCCTT
    CATCAGATTT GTTTGTGTGG TTATTATGAG AATCTGGAAA AAGGAATAAC ATTATTTCGC
    CAGGGTGATA TTGGAACAAA CTGGTATGCT GTCCTGGCAG GGTCTTTGGA TGTTAAAGTA
    TCTGAAACCA GCAGTCACCA GAAATATCGA CAGTACATGG CAGGACTTCT GGCTCCTCCT
    TATGGTGTTA TGGAAACGGG CTCTAACAAT GACAGGATTC CTGACAAGGA GAATACACCT
    CTCATTGAAC CTCACGTTCC TCTTCGTCCT GCTAACACCA TTACCAAGGT CCCTTCAGAG
    AAGATCCTCA GGGCTGGAAA AATTTTACGA AACGCCATTC TCTCTCGAGC ACCTCACATG
    ATTAGAGATA GGAAATACCA CCTAAAGACG TACAGACAAT GCTGTGTGGG AACTGAGCTG
    GTGGACTGGA TGCTGCAGCA GACTCCGTGT GCCCACTCCC GGACTCAAGC TGTTGGGATG
    TGGCAAGTGC TGGTGGAAGA TGGTGTTCTC AACCATGTGG ACCAGGAGCA TCATTTCCAA
    GACAAATATT TATTTTATCG ATTTCTGGAT GACGAGCACG AGGATGCCCC TTTGCCTACT
    GAGGAGGAGA AGAAGGAGTG TGATGAGGAG CTCCAGGACA CCATGCTGCT GCTGTCACAG
    ATGGGCCCCG ATGCCCACAT GAGGATGATC CTCCGCAAAC CACCTGGCCA GAGGACTGTG
    GATGACCTAG AAATTATCTA CGAAGAGCTC CTTCACATTA AAGCCTTATC CCACCTTTCC
    ACCACTGTGA AACGGGAGTT GGCAGGTGTT CTCATTTTTG AGTCGCACGC CAAAGGAGGA
    ACCGTGTTGT TCAACCAGGG GGAAGAAGGT ACCTCCTGGT ACATTATTCT AAAAGGATCG
    GTGAATGTAG TCATTTATGG CAAGGGTGTG GTCTGCACCC TGCATGAGGG AGATGACTTT
    GGCAAGCTAG CACTAGTGAA CGACGCCCCG AGAGCTGCCT CCATCGTCTT GCGAGAAGAT
    AATTGCCATT TCTTAAGAGT GGACAAGGAG GATTTCAACC GAATCCTGAG GGACGTTGAG
    GCGAATACAG TCAGACTTAA AGAACATGAC CAAGATGTCT TGGTGCTGGA GAAGGTCCCA
    GCAGGGAACA GGGCTTCTAA TCAAGGAAAC TCACAGCCTC AGCAAAAGTA TACTGTGATG
    TCAGGAACAC CTGAAAAAAT TTTAGAGCAT TTTCTAGAAA CAATACGCCT TGAGCCAGCT
    TTAAATGAAG CAACAGATTC TGTTTTAAAC GACTTTGTTA TGATGCACTG TGTTTTTATG
    CCAAATATCC AGCTTTGTCC AGCCCTGGTG GCCCATTACC ATGCACAGCC CTCACAAGGT
    ACAGAACAGG AGAAAATGGA TTATGCCCTC AACAATAAGA GACGAGTCAT CCGGCTGGTT
    TTACAGTGGG CCGCCATGTA TGGAGACGTC CTACAAGAGG ATGACGTAGC TATGGCTTTC
    CTGGAGGAGT TTTATGTGTC TGTGTCAGAT GATGCCCGGA TGATTGCTGC CCTCAAGGAG
    CAACTGCCCG AGCTGGAGAA GATCGTCAAG CAAATTTCAG AAGATGCAAA AGCTCCACAA
    AAGAAGCACA AAGTTCTTTT GCAACAGTTC AACACAGGCG ATGAGAGAGC CCAGAAGCGC
    CAGCCTATCC GTGGCTCTGA TGAAGTTCTG TTTAAGGTCT ATTGCATGGA CCACACCTAC
    ACAACCATTC GGGTGCCGGT GGCCGCCTCA GTGAAGGAAG TCATCAGCGC AGTTGCCGAC
    AAACTGGGCT CCGGAGAAGG CCTGATCATA GTCAAGATGA GTTCCGGAGG AGAAAAGGTG
    GTGCTCAAAC CTAATGATGT TTCAGTATTT ACAACGCTCA CCATTAATGG ACGCCTGTTT
    GCTTGCCCGC GAGAGCAATT CGACTCACTG ACTCCCCTGC CAGAACAGGA AGGCCCAACT
    GTTGGAACAG TGGGAACTTT TGAACTGATG AGCTCCAAAG ATTTAGCATA CCAGATGACA
    ATTTATGATT GGGAACTCTT CAACTGTGTG CATGAGCTGG AGCTAATCTA TCACACATTT
    GGAAGGCATA ATTTTAAAAA GACCACAGCA AACTTGGATT TGTTCCTGAG AAGATTTAAT
    GAAATTCAGT TTTGGGTTGT CACCGAGATT TGCCTTTGTC CCCAGCCCAG CAAGCGTGTT
    CAGCTCTTAA AAAAATTTAT TAAGATCGCA GCCCACTGTA AGGAGTACAA AAATCTGAAT
    TCCTTTTTTG CCATCGTCAT GGGACTAAGT AATGTTGCCG TGAGCCGTTT GGCACTAACG
    TGGGAGAAAC TGCCAAGCAA GTTCAAGAAG TTCTATGCGG AGTTTGAAAG CTTAATGGAT
    CCTTCGAGAA ACCACAGGGC CTACCGGCTG ACCATAGCTA AGCTGGACCC CCCTCTCATC
    CCCTTCATGC CTTTGCTCAT TAAAGATATG ACATTTACTC ATGAGGGGAA CAAGACATTC
    ATTGACAATC TAGTAAACTT TGAAAAAATG CGCATGATTG CAAATACTGC CAGAACAGTG
    AGATACTGCA GGAGCCAACC CTTCAATCCT GATGCCGCTC AAGCTAATAA GAACCATCAG
    GATGTCCGGA GTTATGTACG GCAATTAAAT GTGATTGACA ACCAGAGAAC TTTATCACAG
    ATGTCACACA GATTAGAGCC TCGTCGACCA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb52132
    Clone ID Related Accession (Same CDS sequence) XM_023622002.1
    Accession Version XM_023622002.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2634bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product rap guanine nucleotide exchange factor 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGTCACCAG CAATCTGCCA CTTTACGCAT GGATGTAGCC GGAAATATCG ACAGTACATG 
    GCAGGACTTC TGGCTCCTCC TTATGGTGTT ATGGAAACGG GCTCTAACAA TGACAGGATT
    CCTGACAAGG AGAATACACC TCTCATTGAA CCTCACGTTC CTCTTCGTCC TGCTAACACC
    ATTACCAAGG TCCCTTCAGA GAAGATCCTC AGGGCTGGAA AAATTTTACG AAACGCCATT
    CTCTCTCGAG CACCTCACAT GATTAGAGAT AGGAAATACC ACCTAAAGAC GTACAGACAA
    TGCTGTGTGG GAACTGAGCT GGTGGACTGG ATGCTGCAGC AGACTCCGTG TGCCCACTCC
    CGGACTCAAG CTGTTGGGAT GTGGCAAGTG CTGGTGGAAG ATGGTGTTCT CAACCATGTG
    GACCAGGAGC ATCATTTCCA AGACAAATAT TTATTTTATC GATTTCTGGA TGACGAGCAC
    GAGGATGCCC CTTTGCCTAC TGAGGAGGAG AAGAAGGAGT GTGATGAGGA GCTCCAGGAC
    ACCATGCTGC TGCTGTCACA GATGGGCCCC GATGCCCACA TGAGGATGAT CCTCCGCAAA
    CCACCTGGCC AGAGGACTGT GGATGACCTA GAAATTATCT ACGAAGAGCT CCTTCACATT
    AAAGCCTTAT CCCACCTTTC CACCACTGTG AAACGGGAGT TGGCAGGTGT TCTCATTTTT
    GAGTCGCACG CCAAAGGAGG AACCGTGTTG TTCAACCAGG GGGAAGAAGG TACCTCCTGG
    TACATTATTC TAAAAGGATC GGTGAATGTA GTCATTTATG GCAAGGGTGT GGTCTGCACC
    CTGCATGAGG GAGATGACTT TGGCAAGCTA GCACTAGTGA ACGACGCCCC GAGAGCTGCC
    TCCATCGTCT TGCGAGAAGA TAATTGCCAT TTCTTAAGAG TGGACAAGGA GGATTTCAAC
    CGAATCCTGA GGGACGTTGA GGCGAATACA GTCAGACTTA AAGAACATGA CCAAGATGTC
    TTGGTGCTGG AGAAGGTCCC AGCAGGGAAC AGGGCTTCTA ATCAAGGAAA CTCACAGCCT
    CAGCAAAAGT ATACTGTGAT GTCAGGAACA CCTGAAAAAA TTTTAGAGCA TTTTCTAGAA
    ACAATACGCC TTGAGCCAGC TTTAAATGAA GCAACAGATT CTGTTTTAAA CGACTTTGTT
    ATGATGCACT GTGTTTTTAT GCCAAATATC CAGCTTTGTC CAGCCCTGGT GGCCCATTAC
    CATGCACAGC CCTCACAAGG TACAGAACAG GAGAAAATGG ATTATGCCCT CAACAATAAG
    AGACGAGTCA TCCGGCTGGT TTTACAGTGG GCCGCCATGT ATGGAGACGT CCTACAAGAG
    GATGACGTAG CTATGGCTTT CCTGGAGGAG TTTTATGTGT CTGTGTCAGA TGATGCCCGG
    ATGATTGCTG CCCTCAAGGA GCAACTGCCC GAGCTGGAGA AGATCGTCAA GCAAATTTCA
    GAAGATGCAA AAGCTCCACA AAAGAAGCAC AAAGTTCTTT TGCAACAGTT CAACACAGGC
    GATGAGAGAG CCCAGAAGCG CCAGCCTATC CGTGGCTCTG ATGAAGTTCT GTTTAAGGTC
    TATTGCATGG ACCACACCTA CACAACCATT CGGGTGCCGG TGGCCGCCTC AGTGAAGGAA
    GTCATCAGCG CAGTTGCCGA CAAACTGGGC TCCGGAGAAG GCCTGATCAT AGTCAAGATG
    AGTTCCGGAG GAGAAAAGGT GGTGCTCAAA CCTAATGATG TTTCAGTATT TACAACGCTC
    ACCATTAATG GACGCCTGTT TGCTTGCCCG CGAGAGCAAT TCGACTCACT GACTCCCCTG
    CCAGAACAGG AAGGCCCAAC TGTTGGAACA GTGGGAACTT TTGAACTGAT GAGCTCCAAA
    GATTTAGCAT ACCAGATGAC AATTTATGAT TGGGAACTCT TCAACTGTGT GCATGAGCTG
    GAGCTAATCT ATCACACATT TGGAAGGCAT AATTTTAAAA AGACCACAGC AAACTTGGAT
    TTGTTCCTGA GAAGATTTAA TGAAATTCAG TTTTGGGTTG TCACCGAGAT TTGCCTTTGT
    CCCCAGCCCA GCAAGCGTGT TCAGCTCTTA AAAAAATTTA TTAAGATCGC AGCCCACTGT
    AAGGAGTACA AAAATCTGAA TTCCTTTTTT GCCATCGTCA TGGGACTAAG TAATGTTGCC
    GTGAGCCGTT TGGCACTAAC GTGGGAGAAA CTGCCAAGCA AGTTCAAGAA GTTCTATGCG
    GAGTTTGAAA GCTTAATGGA TCCTTCGAGA AACCACAGGG CCTACCGGCT GACCATAGCT
    AAGCTGGACC CCCCTCTCAT CCCCTTCATG CCTTTGCTCA TTAAAGATAT GACATTTACT
    CATGAGGGGA ACAAGACATT CATTGACAAT CTAGTAAACT TTGAAAAAAT GCGCATGATT
    GCAAATACTG CCAGAACAGT GAGATACTGC AGGAGCCAAC CCTTCAATCC TGATGCCGCT
    CAAGCTAATA AGAACCATCA GGATGTCCGG AGTTATGTAC GGCAATTAAA TGTGATTGAC
    AACCAGAGAA CTTTATCACA GATGTCACAC AGATTAGAGC CTCGTCGACC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023477770.1
    CDS10564..13197
    Translation

    Target ORF information:

    RefSeq Version XM_023622002.1
    Organism Equus caballus(horse)
    Definition Equus caballus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023622002.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGTCACCAG CAATCTGCCA CTTTACGCAT GGATGTAGCC GGAAATATCG ACAGTACATG 
    GCAGGACTTC TGGCTCCTCC TTATGGTGTT ATGGAAACGG GCTCTAACAA TGACAGGATT
    CCTGACAAGG AGAATACACC TCTCATTGAA CCTCACGTTC CTCTTCGTCC TGCTAACACC
    ATTACCAAGG TCCCTTCAGA GAAGATCCTC AGGGCTGGAA AAATTTTACG AAACGCCATT
    CTCTCTCGAG CACCTCACAT GATTAGAGAT AGGAAATACC ACCTAAAGAC GTACAGACAA
    TGCTGTGTGG GAACTGAGCT GGTGGACTGG ATGCTGCAGC AGACTCCGTG TGCCCACTCC
    CGGACTCAAG CTGTTGGGAT GTGGCAAGTG CTGGTGGAAG ATGGTGTTCT CAACCATGTG
    GACCAGGAGC ATCATTTCCA AGACAAATAT TTATTTTATC GATTTCTGGA TGACGAGCAC
    GAGGATGCCC CTTTGCCTAC TGAGGAGGAG AAGAAGGAGT GTGATGAGGA GCTCCAGGAC
    ACCATGCTGC TGCTGTCACA GATGGGCCCC GATGCCCACA TGAGGATGAT CCTCCGCAAA
    CCACCTGGCC AGAGGACTGT GGATGACCTA GAAATTATCT ACGAAGAGCT CCTTCACATT
    AAAGCCTTAT CCCACCTTTC CACCACTGTG AAACGGGAGT TGGCAGGTGT TCTCATTTTT
    GAGTCGCACG CCAAAGGAGG AACCGTGTTG TTCAACCAGG GGGAAGAAGG TACCTCCTGG
    TACATTATTC TAAAAGGATC GGTGAATGTA GTCATTTATG GCAAGGGTGT GGTCTGCACC
    CTGCATGAGG GAGATGACTT TGGCAAGCTA GCACTAGTGA ACGACGCCCC GAGAGCTGCC
    TCCATCGTCT TGCGAGAAGA TAATTGCCAT TTCTTAAGAG TGGACAAGGA GGATTTCAAC
    CGAATCCTGA GGGACGTTGA GGCGAATACA GTCAGACTTA AAGAACATGA CCAAGATGTC
    TTGGTGCTGG AGAAGGTCCC AGCAGGGAAC AGGGCTTCTA ATCAAGGAAA CTCACAGCCT
    CAGCAAAAGT ATACTGTGAT GTCAGGAACA CCTGAAAAAA TTTTAGAGCA TTTTCTAGAA
    ACAATACGCC TTGAGCCAGC TTTAAATGAA GCAACAGATT CTGTTTTAAA CGACTTTGTT
    ATGATGCACT GTGTTTTTAT GCCAAATATC CAGCTTTGTC CAGCCCTGGT GGCCCATTAC
    CATGCACAGC CCTCACAAGG TACAGAACAG GAGAAAATGG ATTATGCCCT CAACAATAAG
    AGACGAGTCA TCCGGCTGGT TTTACAGTGG GCCGCCATGT ATGGAGACGT CCTACAAGAG
    GATGACGTAG CTATGGCTTT CCTGGAGGAG TTTTATGTGT CTGTGTCAGA TGATGCCCGG
    ATGATTGCTG CCCTCAAGGA GCAACTGCCC GAGCTGGAGA AGATCGTCAA GCAAATTTCA
    GAAGATGCAA AAGCTCCACA AAAGAAGCAC AAAGTTCTTT TGCAACAGTT CAACACAGGC
    GATGAGAGAG CCCAGAAGCG CCAGCCTATC CGTGGCTCTG ATGAAGTTCT GTTTAAGGTC
    TATTGCATGG ACCACACCTA CACAACCATT CGGGTGCCGG TGGCCGCCTC AGTGAAGGAA
    GTCATCAGCG CAGTTGCCGA CAAACTGGGC TCCGGAGAAG GCCTGATCAT AGTCAAGATG
    AGTTCCGGAG GAGAAAAGGT GGTGCTCAAA CCTAATGATG TTTCAGTATT TACAACGCTC
    ACCATTAATG GACGCCTGTT TGCTTGCCCG CGAGAGCAAT TCGACTCACT GACTCCCCTG
    CCAGAACAGG AAGGCCCAAC TGTTGGAACA GTGGGAACTT TTGAACTGAT GAGCTCCAAA
    GATTTAGCAT ACCAGATGAC AATTTATGAT TGGGAACTCT TCAACTGTGT GCATGAGCTG
    GAGCTAATCT ATCACACATT TGGAAGGCAT AATTTTAAAA AGACCACAGC AAACTTGGAT
    TTGTTCCTGA GAAGATTTAA TGAAATTCAG TTTTGGGTTG TCACCGAGAT TTGCCTTTGT
    CCCCAGCCCA GCAAGCGTGT TCAGCTCTTA AAAAAATTTA TTAAGATCGC AGCCCACTGT
    AAGGAGTACA AAAATCTGAA TTCCTTTTTT GCCATCGTCA TGGGACTAAG TAATGTTGCC
    GTGAGCCGTT TGGCACTAAC GTGGGAGAAA CTGCCAAGCA AGTTCAAGAA GTTCTATGCG
    GAGTTTGAAA GCTTAATGGA TCCTTCGAGA AACCACAGGG CCTACCGGCT GACCATAGCT
    AAGCTGGACC CCCCTCTCAT CCCCTTCATG CCTTTGCTCA TTAAAGATAT GACATTTACT
    CATGAGGGGA ACAAGACATT CATTGACAAT CTAGTAAACT TTGAAAAAAT GCGCATGATT
    GCAAATACTG CCAGAACAGT GAGATACTGC AGGAGCCAAC CCTTCAATCC TGATGCCGCT
    CAAGCTAATA AGAACCATCA GGATGTCCGG AGTTATGTAC GGCAATTAAA TGTGATTGAC
    AACCAGAGAA CTTTATCACA GATGTCACAC AGATTAGAGC CTCGTCGACC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb52133
    Clone ID Related Accession (Same CDS sequence) XM_023622003.1
    Accession Version XM_023622003.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product rap guanine nucleotide exchange factor 4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCCCTACC AGAAATATCG ACAGTACATG GCAGGACTTC TGGCTCCTCC TTATGGTGTT 
    ATGGAAACGG GCTCTAACAA TGACAGGATT CCTGACAAGG AGAATACACC TCTCATTGAA
    CCTCACGTTC CTCTTCGTCC TGCTAACACC ATTACCAAGG TCCCTTCAGA GAAGATCCTC
    AGGGCTGGAA AAATTTTACG AAACGCCATT CTCTCTCGAG CACCTCACAT GATTAGAGAT
    AGGAAATACC ACCTAAAGAC GTACAGACAA TGCTGTGTGG GAACTGAGCT GGTGGACTGG
    ATGCTGCAGC AGACTCCGTG TGCCCACTCC CGGACTCAAG CTGTTGGGAT GTGGCAAGTG
    CTGGTGGAAG ATGGTGTTCT CAACCATGTG GACCAGGAGC ATCATTTCCA AGACAAATAT
    TTATTTTATC GATTTCTGGA TGACGAGCAC GAGGATGCCC CTTTGCCTAC TGAGGAGGAG
    AAGAAGGAGT GTGATGAGGA GCTCCAGGAC ACCATGCTGC TGCTGTCACA GATGGGCCCC
    GATGCCCACA TGAGGATGAT CCTCCGCAAA CCACCTGGCC AGAGGACTGT GGATGACCTA
    GAAATTATCT ACGAAGAGCT CCTTCACATT AAAGCCTTAT CCCACCTTTC CACCACTGTG
    AAACGGGAGT TGGCAGGTGT TCTCATTTTT GAGTCGCACG CCAAAGGAGG AACCGTGTTG
    TTCAACCAGG GGGAAGAAGG TACCTCCTGG TACATTATTC TAAAAGGATC GGTGAATGTA
    GTCATTTATG GCAAGGGTGT GGTCTGCACC CTGCATGAGG GAGATGACTT TGGCAAGCTA
    GCACTAGTGA ACGACGCCCC GAGAGCTGCC TCCATCGTCT TGCGAGAAGA TAATTGCCAT
    TTCTTAAGAG TGGACAAGGA GGATTTCAAC CGAATCCTGA GGGACGTTGA GGCGAATACA
    GTCAGACTTA AAGAACATGA CCAAGATGTC TTGGTGCTGG AGAAGGTCCC AGCAGGGAAC
    AGGGCTTCTA ATCAAGGAAA CTCACAGCCT CAGCAAAAGT ATACTGTGAT GTCAGGAACA
    CCTGAAAAAA TTTTAGAGCA TTTTCTAGAA ACAATACGCC TTGAGCCAGC TTTAAATGAA
    GCAACAGATT CTGTTTTAAA CGACTTTGTT ATGATGCACT GTGTTTTTAT GCCAAATATC
    CAGCTTTGTC CAGCCCTGGT GGCCCATTAC CATGCACAGC CCTCACAAGG TACAGAACAG
    GAGAAAATGG ATTATGCCCT CAACAATAAG AGACGAGTCA TCCGGCTGGT TTTACAGTGG
    GCCGCCATGT ATGGAGACGT CCTACAAGAG GATGACGTAG CTATGGCTTT CCTGGAGGAG
    TTTTATGTGT CTGTGTCAGA TGATGCCCGG ATGATTGCTG CCCTCAAGGA GCAACTGCCC
    GAGCTGGAGA AGATCGTCAA GCAAATTTCA GAAGATGCAA AAGCTCCACA AAAGAAGCAC
    AAAGTTCTTT TGCAACAGTT CAACACAGGC GATGAGAGAG CCCAGAAGCG CCAGCCTATC
    CGTGGCTCTG ATGAAGTTCT GTTTAAGGTC TATTGCATGG ACCACACCTA CACAACCATT
    CGGGTGCCGG TGGCCGCCTC AGTGAAGGAA GTCATCAGCG CAGTTGCCGA CAAACTGGGC
    TCCGGAGAAG GCCTGATCAT AGTCAAGATG AGTTCCGGAG GAGAAAAGGT GGTGCTCAAA
    CCTAATGATG TTTCAGTATT TACAACGCTC ACCATTAATG GACGCCTGTT TGCTTGCCCG
    CGAGAGCAAT TCGACTCACT GACTCCCCTG CCAGAACAGG AAGGCCCAAC TGTTGGAACA
    GTGGGAACTT TTGAACTGAT GAGCTCCAAA GATTTAGCAT ACCAGATGAC AATTTATGAT
    TGGGAACTCT TCAACTGTGT GCATGAGCTG GAGCTAATCT ATCACACATT TGGAAGGCAT
    AATTTTAAAA AGACCACAGC AAACTTGGAT TTGTTCCTGA GAAGATTTAA TGAAATTCAG
    TTTTGGGTTG TCACCGAGAT TTGCCTTTGT CCCCAGCCCA GCAAGCGTGT TCAGCTCTTA
    AAAAAATTTA TTAAGATCGC AGCCCACTGT AAGGAGTACA AAAATCTGAA TTCCTTTTTT
    GCCATCGTCA TGGGACTAAG TAATGTTGCC GTGAGCCGTT TGGCACTAAC GTGGGAGAAA
    CTGCCAAGCA AGTTCAAGAA GTTCTATGCG GAGTTTGAAA GCTTAATGGA TCCTTCGAGA
    AACCACAGGG CCTACCGGCT GACCATAGCT AAGCTGGACC CCCCTCTCAT CCCCTTCATG
    CCTTTGCTCA TTAAAGATAT GACATTTACT CATGAGGGGA ACAAGACATT CATTGACAAT
    CTAGTAAACT TTGAAAAAAT GCGCATGATT GCAAATACTG CCAGAACAGT GAGATACTGC
    AGGAGCCAAC CCTTCAATCC TGATGCCGCT CAAGCTAATA AGAACCATCA GGATGTCCGG
    AGTTATGTAC GGCAATTAAA TGTGATTGAC AACCAGAGAA CTTTATCACA GATGTCACAC
    AGATTAGAGC CTCGTCGACC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023477771.1
    CDS2952..5555
    Translation

    Target ORF information:

    RefSeq Version XM_023622003.1
    Organism Equus caballus(horse)
    Definition Equus caballus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023622003.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCCCTACC AGAAATATCG ACAGTACATG GCAGGACTTC TGGCTCCTCC TTATGGTGTT 
    ATGGAAACGG GCTCTAACAA TGACAGGATT CCTGACAAGG AGAATACACC TCTCATTGAA
    CCTCACGTTC CTCTTCGTCC TGCTAACACC ATTACCAAGG TCCCTTCAGA GAAGATCCTC
    AGGGCTGGAA AAATTTTACG AAACGCCATT CTCTCTCGAG CACCTCACAT GATTAGAGAT
    AGGAAATACC ACCTAAAGAC GTACAGACAA TGCTGTGTGG GAACTGAGCT GGTGGACTGG
    ATGCTGCAGC AGACTCCGTG TGCCCACTCC CGGACTCAAG CTGTTGGGAT GTGGCAAGTG
    CTGGTGGAAG ATGGTGTTCT CAACCATGTG GACCAGGAGC ATCATTTCCA AGACAAATAT
    TTATTTTATC GATTTCTGGA TGACGAGCAC GAGGATGCCC CTTTGCCTAC TGAGGAGGAG
    AAGAAGGAGT GTGATGAGGA GCTCCAGGAC ACCATGCTGC TGCTGTCACA GATGGGCCCC
    GATGCCCACA TGAGGATGAT CCTCCGCAAA CCACCTGGCC AGAGGACTGT GGATGACCTA
    GAAATTATCT ACGAAGAGCT CCTTCACATT AAAGCCTTAT CCCACCTTTC CACCACTGTG
    AAACGGGAGT TGGCAGGTGT TCTCATTTTT GAGTCGCACG CCAAAGGAGG AACCGTGTTG
    TTCAACCAGG GGGAAGAAGG TACCTCCTGG TACATTATTC TAAAAGGATC GGTGAATGTA
    GTCATTTATG GCAAGGGTGT GGTCTGCACC CTGCATGAGG GAGATGACTT TGGCAAGCTA
    GCACTAGTGA ACGACGCCCC GAGAGCTGCC TCCATCGTCT TGCGAGAAGA TAATTGCCAT
    TTCTTAAGAG TGGACAAGGA GGATTTCAAC CGAATCCTGA GGGACGTTGA GGCGAATACA
    GTCAGACTTA AAGAACATGA CCAAGATGTC TTGGTGCTGG AGAAGGTCCC AGCAGGGAAC
    AGGGCTTCTA ATCAAGGAAA CTCACAGCCT CAGCAAAAGT ATACTGTGAT GTCAGGAACA
    CCTGAAAAAA TTTTAGAGCA TTTTCTAGAA ACAATACGCC TTGAGCCAGC TTTAAATGAA
    GCAACAGATT CTGTTTTAAA CGACTTTGTT ATGATGCACT GTGTTTTTAT GCCAAATATC
    CAGCTTTGTC CAGCCCTGGT GGCCCATTAC CATGCACAGC CCTCACAAGG TACAGAACAG
    GAGAAAATGG ATTATGCCCT CAACAATAAG AGACGAGTCA TCCGGCTGGT TTTACAGTGG
    GCCGCCATGT ATGGAGACGT CCTACAAGAG GATGACGTAG CTATGGCTTT CCTGGAGGAG
    TTTTATGTGT CTGTGTCAGA TGATGCCCGG ATGATTGCTG CCCTCAAGGA GCAACTGCCC
    GAGCTGGAGA AGATCGTCAA GCAAATTTCA GAAGATGCAA AAGCTCCACA AAAGAAGCAC
    AAAGTTCTTT TGCAACAGTT CAACACAGGC GATGAGAGAG CCCAGAAGCG CCAGCCTATC
    CGTGGCTCTG ATGAAGTTCT GTTTAAGGTC TATTGCATGG ACCACACCTA CACAACCATT
    CGGGTGCCGG TGGCCGCCTC AGTGAAGGAA GTCATCAGCG CAGTTGCCGA CAAACTGGGC
    TCCGGAGAAG GCCTGATCAT AGTCAAGATG AGTTCCGGAG GAGAAAAGGT GGTGCTCAAA
    CCTAATGATG TTTCAGTATT TACAACGCTC ACCATTAATG GACGCCTGTT TGCTTGCCCG
    CGAGAGCAAT TCGACTCACT GACTCCCCTG CCAGAACAGG AAGGCCCAAC TGTTGGAACA
    GTGGGAACTT TTGAACTGAT GAGCTCCAAA GATTTAGCAT ACCAGATGAC AATTTATGAT
    TGGGAACTCT TCAACTGTGT GCATGAGCTG GAGCTAATCT ATCACACATT TGGAAGGCAT
    AATTTTAAAA AGACCACAGC AAACTTGGAT TTGTTCCTGA GAAGATTTAA TGAAATTCAG
    TTTTGGGTTG TCACCGAGAT TTGCCTTTGT CCCCAGCCCA GCAAGCGTGT TCAGCTCTTA
    AAAAAATTTA TTAAGATCGC AGCCCACTGT AAGGAGTACA AAAATCTGAA TTCCTTTTTT
    GCCATCGTCA TGGGACTAAG TAATGTTGCC GTGAGCCGTT TGGCACTAAC GTGGGAGAAA
    CTGCCAAGCA AGTTCAAGAA GTTCTATGCG GAGTTTGAAA GCTTAATGGA TCCTTCGAGA
    AACCACAGGG CCTACCGGCT GACCATAGCT AAGCTGGACC CCCCTCTCAT CCCCTTCATG
    CCTTTGCTCA TTAAAGATAT GACATTTACT CATGAGGGGA ACAAGACATT CATTGACAAT
    CTAGTAAACT TTGAAAAAAT GCGCATGATT GCAAATACTG CCAGAACAGT GAGATACTGC
    AGGAGCCAAC CCTTCAATCC TGATGCCGCT CAAGCTAATA AGAACCATCA GGATGTCCGG
    AGTTATGTAC GGCAATTAAA TGTGATTGAC AACCAGAGAA CTTTATCACA GATGTCACAC
    AGATTAGAGC CTCGTCGACC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb52134
    Clone ID Related Accession (Same CDS sequence) XM_023622005.1 , XM_023622006.1 , XM_023622004.1
    Accession Version XM_023622005.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product rap guanine nucleotide exchange factor 4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGGAC TTCTGGCTCC TCCTTATGGT GTTATGGAAA CGGGCTCTAA CAATGACAGG 
    ATTCCTGACA AGGAGAATAC ACCTCTCATT GAACCTCACG TTCCTCTTCG TCCTGCTAAC
    ACCATTACCA AGGTCCCTTC AGAGAAGATC CTCAGGGCTG GAAAAATTTT ACGAAACGCC
    ATTCTCTCTC GAGCACCTCA CATGATTAGA GATAGGAAAT ACCACCTAAA GACGTACAGA
    CAATGCTGTG TGGGAACTGA GCTGGTGGAC TGGATGCTGC AGCAGACTCC GTGTGCCCAC
    TCCCGGACTC AAGCTGTTGG GATGTGGCAA GTGCTGGTGG AAGATGGTGT TCTCAACCAT
    GTGGACCAGG AGCATCATTT CCAAGACAAA TATTTATTTT ATCGATTTCT GGATGACGAG
    CACGAGGATG CCCCTTTGCC TACTGAGGAG GAGAAGAAGG AGTGTGATGA GGAGCTCCAG
    GACACCATGC TGCTGCTGTC ACAGATGGGC CCCGATGCCC ACATGAGGAT GATCCTCCGC
    AAACCACCTG GCCAGAGGAC TGTGGATGAC CTAGAAATTA TCTACGAAGA GCTCCTTCAC
    ATTAAAGCCT TATCCCACCT TTCCACCACT GTGAAACGGG AGTTGGCAGG TGTTCTCATT
    TTTGAGTCGC ACGCCAAAGG AGGAACCGTG TTGTTCAACC AGGGGGAAGA AGGTACCTCC
    TGGTACATTA TTCTAAAAGG ATCGGTGAAT GTAGTCATTT ATGGCAAGGG TGTGGTCTGC
    ACCCTGCATG AGGGAGATGA CTTTGGCAAG CTAGCACTAG TGAACGACGC CCCGAGAGCT
    GCCTCCATCG TCTTGCGAGA AGATAATTGC CATTTCTTAA GAGTGGACAA GGAGGATTTC
    AACCGAATCC TGAGGGACGT TGAGGCGAAT ACAGTCAGAC TTAAAGAACA TGACCAAGAT
    GTCTTGGTGC TGGAGAAGGT CCCAGCAGGG AACAGGGCTT CTAATCAAGG AAACTCACAG
    CCTCAGCAAA AGTATACTGT GATGTCAGGA ACACCTGAAA AAATTTTAGA GCATTTTCTA
    GAAACAATAC GCCTTGAGCC AGCTTTAAAT GAAGCAACAG ATTCTGTTTT AAACGACTTT
    GTTATGATGC ACTGTGTTTT TATGCCAAAT ATCCAGCTTT GTCCAGCCCT GGTGGCCCAT
    TACCATGCAC AGCCCTCACA AGGTACAGAA CAGGAGAAAA TGGATTATGC CCTCAACAAT
    AAGAGACGAG TCATCCGGCT GGTTTTACAG TGGGCCGCCA TGTATGGAGA CGTCCTACAA
    GAGGATGACG TAGCTATGGC TTTCCTGGAG GAGTTTTATG TGTCTGTGTC AGATGATGCC
    CGGATGATTG CTGCCCTCAA GGAGCAACTG CCCGAGCTGG AGAAGATCGT CAAGCAAATT
    TCAGAAGATG CAAAAGCTCC ACAAAAGAAG CACAAAGTTC TTTTGCAACA GTTCAACACA
    GGCGATGAGA GAGCCCAGAA GCGCCAGCCT ATCCGTGGCT CTGATGAAGT TCTGTTTAAG
    GTCTATTGCA TGGACCACAC CTACACAACC ATTCGGGTGC CGGTGGCCGC CTCAGTGAAG
    GAAGTCATCA GCGCAGTTGC CGACAAACTG GGCTCCGGAG AAGGCCTGAT CATAGTCAAG
    ATGAGTTCCG GAGGAGAAAA GGTGGTGCTC AAACCTAATG ATGTTTCAGT ATTTACAACG
    CTCACCATTA ATGGACGCCT GTTTGCTTGC CCGCGAGAGC AATTCGACTC ACTGACTCCC
    CTGCCAGAAC AGGAAGGCCC AACTGTTGGA ACAGTGGGAA CTTTTGAACT GATGAGCTCC
    AAAGATTTAG CATACCAGAT GACAATTTAT GATTGGGAAC TCTTCAACTG TGTGCATGAG
    CTGGAGCTAA TCTATCACAC ATTTGGAAGG CATAATTTTA AAAAGACCAC AGCAAACTTG
    GATTTGTTCC TGAGAAGATT TAATGAAATT CAGTTTTGGG TTGTCACCGA GATTTGCCTT
    TGTCCCCAGC CCAGCAAGCG TGTTCAGCTC TTAAAAAAAT TTATTAAGAT CGCAGCCCAC
    TGTAAGGAGT ACAAAAATCT GAATTCCTTT TTTGCCATCG TCATGGGACT AAGTAATGTT
    GCCGTGAGCC GTTTGGCACT AACGTGGGAG AAACTGCCAA GCAAGTTCAA GAAGTTCTAT
    GCGGAGTTTG AAAGCTTAAT GGATCCTTCG AGAAACCACA GGGCCTACCG GCTGACCATA
    GCTAAGCTGG ACCCCCCTCT CATCCCCTTC ATGCCTTTGC TCATTAAAGA TATGACATTT
    ACTCATGAGG GGAACAAGAC ATTCATTGAC AATCTAGTAA ACTTTGAAAA AATGCGCATG
    ATTGCAAATA CTGCCAGAAC AGTGAGATAC TGCAGGAGCC AACCCTTCAA TCCTGATGCC
    GCTCAAGCTA ATAAGAACCA TCAGGATGTC CGGAGTTATG TACGGCAATT AAATGTGATT
    GACAACCAGA GAACTTTATC ACAGATGTCA CACAGATTAG AGCCTCGTCG ACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023477773.1
    CDS8746..11322
    Translation

    Target ORF information:

    RefSeq Version XM_023622005.1
    Organism Equus caballus(horse)
    Definition Equus caballus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023622005.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGGAC TTCTGGCTCC TCCTTATGGT GTTATGGAAA CGGGCTCTAA CAATGACAGG 
    ATTCCTGACA AGGAGAATAC ACCTCTCATT GAACCTCACG TTCCTCTTCG TCCTGCTAAC
    ACCATTACCA AGGTCCCTTC AGAGAAGATC CTCAGGGCTG GAAAAATTTT ACGAAACGCC
    ATTCTCTCTC GAGCACCTCA CATGATTAGA GATAGGAAAT ACCACCTAAA GACGTACAGA
    CAATGCTGTG TGGGAACTGA GCTGGTGGAC TGGATGCTGC AGCAGACTCC GTGTGCCCAC
    TCCCGGACTC AAGCTGTTGG GATGTGGCAA GTGCTGGTGG AAGATGGTGT TCTCAACCAT
    GTGGACCAGG AGCATCATTT CCAAGACAAA TATTTATTTT ATCGATTTCT GGATGACGAG
    CACGAGGATG CCCCTTTGCC TACTGAGGAG GAGAAGAAGG AGTGTGATGA GGAGCTCCAG
    GACACCATGC TGCTGCTGTC ACAGATGGGC CCCGATGCCC ACATGAGGAT GATCCTCCGC
    AAACCACCTG GCCAGAGGAC TGTGGATGAC CTAGAAATTA TCTACGAAGA GCTCCTTCAC
    ATTAAAGCCT TATCCCACCT TTCCACCACT GTGAAACGGG AGTTGGCAGG TGTTCTCATT
    TTTGAGTCGC ACGCCAAAGG AGGAACCGTG TTGTTCAACC AGGGGGAAGA AGGTACCTCC
    TGGTACATTA TTCTAAAAGG ATCGGTGAAT GTAGTCATTT ATGGCAAGGG TGTGGTCTGC
    ACCCTGCATG AGGGAGATGA CTTTGGCAAG CTAGCACTAG TGAACGACGC CCCGAGAGCT
    GCCTCCATCG TCTTGCGAGA AGATAATTGC CATTTCTTAA GAGTGGACAA GGAGGATTTC
    AACCGAATCC TGAGGGACGT TGAGGCGAAT ACAGTCAGAC TTAAAGAACA TGACCAAGAT
    GTCTTGGTGC TGGAGAAGGT CCCAGCAGGG AACAGGGCTT CTAATCAAGG AAACTCACAG
    CCTCAGCAAA AGTATACTGT GATGTCAGGA ACACCTGAAA AAATTTTAGA GCATTTTCTA
    GAAACAATAC GCCTTGAGCC AGCTTTAAAT GAAGCAACAG ATTCTGTTTT AAACGACTTT
    GTTATGATGC ACTGTGTTTT TATGCCAAAT ATCCAGCTTT GTCCAGCCCT GGTGGCCCAT
    TACCATGCAC AGCCCTCACA AGGTACAGAA CAGGAGAAAA TGGATTATGC CCTCAACAAT
    AAGAGACGAG TCATCCGGCT GGTTTTACAG TGGGCCGCCA TGTATGGAGA CGTCCTACAA
    GAGGATGACG TAGCTATGGC TTTCCTGGAG GAGTTTTATG TGTCTGTGTC AGATGATGCC
    CGGATGATTG CTGCCCTCAA GGAGCAACTG CCCGAGCTGG AGAAGATCGT CAAGCAAATT
    TCAGAAGATG CAAAAGCTCC ACAAAAGAAG CACAAAGTTC TTTTGCAACA GTTCAACACA
    GGCGATGAGA GAGCCCAGAA GCGCCAGCCT ATCCGTGGCT CTGATGAAGT TCTGTTTAAG
    GTCTATTGCA TGGACCACAC CTACACAACC ATTCGGGTGC CGGTGGCCGC CTCAGTGAAG
    GAAGTCATCA GCGCAGTTGC CGACAAACTG GGCTCCGGAG AAGGCCTGAT CATAGTCAAG
    ATGAGTTCCG GAGGAGAAAA GGTGGTGCTC AAACCTAATG ATGTTTCAGT ATTTACAACG
    CTCACCATTA ATGGACGCCT GTTTGCTTGC CCGCGAGAGC AATTCGACTC ACTGACTCCC
    CTGCCAGAAC AGGAAGGCCC AACTGTTGGA ACAGTGGGAA CTTTTGAACT GATGAGCTCC
    AAAGATTTAG CATACCAGAT GACAATTTAT GATTGGGAAC TCTTCAACTG TGTGCATGAG
    CTGGAGCTAA TCTATCACAC ATTTGGAAGG CATAATTTTA AAAAGACCAC AGCAAACTTG
    GATTTGTTCC TGAGAAGATT TAATGAAATT CAGTTTTGGG TTGTCACCGA GATTTGCCTT
    TGTCCCCAGC CCAGCAAGCG TGTTCAGCTC TTAAAAAAAT TTATTAAGAT CGCAGCCCAC
    TGTAAGGAGT ACAAAAATCT GAATTCCTTT TTTGCCATCG TCATGGGACT AAGTAATGTT
    GCCGTGAGCC GTTTGGCACT AACGTGGGAG AAACTGCCAA GCAAGTTCAA GAAGTTCTAT
    GCGGAGTTTG AAAGCTTAAT GGATCCTTCG AGAAACCACA GGGCCTACCG GCTGACCATA
    GCTAAGCTGG ACCCCCCTCT CATCCCCTTC ATGCCTTTGC TCATTAAAGA TATGACATTT
    ACTCATGAGG GGAACAAGAC ATTCATTGAC AATCTAGTAA ACTTTGAAAA AATGCGCATG
    ATTGCAAATA CTGCCAGAAC AGTGAGATAC TGCAGGAGCC AACCCTTCAA TCCTGATGCC
    GCTCAAGCTA ATAAGAACCA TCAGGATGTC CGGAGTTATG TACGGCAATT AAATGTGATT
    GACAACCAGA GAACTTTATC ACAGATGTCA CACAGATTAG AGCCTCGTCG ACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb52134
    Clone ID Related Accession (Same CDS sequence) XM_023622005.1 , XM_023622006.1 , XM_023622004.1
    Accession Version XM_023622006.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product rap guanine nucleotide exchange factor 4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGGAC TTCTGGCTCC TCCTTATGGT GTTATGGAAA CGGGCTCTAA CAATGACAGG 
    ATTCCTGACA AGGAGAATAC ACCTCTCATT GAACCTCACG TTCCTCTTCG TCCTGCTAAC
    ACCATTACCA AGGTCCCTTC AGAGAAGATC CTCAGGGCTG GAAAAATTTT ACGAAACGCC
    ATTCTCTCTC GAGCACCTCA CATGATTAGA GATAGGAAAT ACCACCTAAA GACGTACAGA
    CAATGCTGTG TGGGAACTGA GCTGGTGGAC TGGATGCTGC AGCAGACTCC GTGTGCCCAC
    TCCCGGACTC AAGCTGTTGG GATGTGGCAA GTGCTGGTGG AAGATGGTGT TCTCAACCAT
    GTGGACCAGG AGCATCATTT CCAAGACAAA TATTTATTTT ATCGATTTCT GGATGACGAG
    CACGAGGATG CCCCTTTGCC TACTGAGGAG GAGAAGAAGG AGTGTGATGA GGAGCTCCAG
    GACACCATGC TGCTGCTGTC ACAGATGGGC CCCGATGCCC ACATGAGGAT GATCCTCCGC
    AAACCACCTG GCCAGAGGAC TGTGGATGAC CTAGAAATTA TCTACGAAGA GCTCCTTCAC
    ATTAAAGCCT TATCCCACCT TTCCACCACT GTGAAACGGG AGTTGGCAGG TGTTCTCATT
    TTTGAGTCGC ACGCCAAAGG AGGAACCGTG TTGTTCAACC AGGGGGAAGA AGGTACCTCC
    TGGTACATTA TTCTAAAAGG ATCGGTGAAT GTAGTCATTT ATGGCAAGGG TGTGGTCTGC
    ACCCTGCATG AGGGAGATGA CTTTGGCAAG CTAGCACTAG TGAACGACGC CCCGAGAGCT
    GCCTCCATCG TCTTGCGAGA AGATAATTGC CATTTCTTAA GAGTGGACAA GGAGGATTTC
    AACCGAATCC TGAGGGACGT TGAGGCGAAT ACAGTCAGAC TTAAAGAACA TGACCAAGAT
    GTCTTGGTGC TGGAGAAGGT CCCAGCAGGG AACAGGGCTT CTAATCAAGG AAACTCACAG
    CCTCAGCAAA AGTATACTGT GATGTCAGGA ACACCTGAAA AAATTTTAGA GCATTTTCTA
    GAAACAATAC GCCTTGAGCC AGCTTTAAAT GAAGCAACAG ATTCTGTTTT AAACGACTTT
    GTTATGATGC ACTGTGTTTT TATGCCAAAT ATCCAGCTTT GTCCAGCCCT GGTGGCCCAT
    TACCATGCAC AGCCCTCACA AGGTACAGAA CAGGAGAAAA TGGATTATGC CCTCAACAAT
    AAGAGACGAG TCATCCGGCT GGTTTTACAG TGGGCCGCCA TGTATGGAGA CGTCCTACAA
    GAGGATGACG TAGCTATGGC TTTCCTGGAG GAGTTTTATG TGTCTGTGTC AGATGATGCC
    CGGATGATTG CTGCCCTCAA GGAGCAACTG CCCGAGCTGG AGAAGATCGT CAAGCAAATT
    TCAGAAGATG CAAAAGCTCC ACAAAAGAAG CACAAAGTTC TTTTGCAACA GTTCAACACA
    GGCGATGAGA GAGCCCAGAA GCGCCAGCCT ATCCGTGGCT CTGATGAAGT TCTGTTTAAG
    GTCTATTGCA TGGACCACAC CTACACAACC ATTCGGGTGC CGGTGGCCGC CTCAGTGAAG
    GAAGTCATCA GCGCAGTTGC CGACAAACTG GGCTCCGGAG AAGGCCTGAT CATAGTCAAG
    ATGAGTTCCG GAGGAGAAAA GGTGGTGCTC AAACCTAATG ATGTTTCAGT ATTTACAACG
    CTCACCATTA ATGGACGCCT GTTTGCTTGC CCGCGAGAGC AATTCGACTC ACTGACTCCC
    CTGCCAGAAC AGGAAGGCCC AACTGTTGGA ACAGTGGGAA CTTTTGAACT GATGAGCTCC
    AAAGATTTAG CATACCAGAT GACAATTTAT GATTGGGAAC TCTTCAACTG TGTGCATGAG
    CTGGAGCTAA TCTATCACAC ATTTGGAAGG CATAATTTTA AAAAGACCAC AGCAAACTTG
    GATTTGTTCC TGAGAAGATT TAATGAAATT CAGTTTTGGG TTGTCACCGA GATTTGCCTT
    TGTCCCCAGC CCAGCAAGCG TGTTCAGCTC TTAAAAAAAT TTATTAAGAT CGCAGCCCAC
    TGTAAGGAGT ACAAAAATCT GAATTCCTTT TTTGCCATCG TCATGGGACT AAGTAATGTT
    GCCGTGAGCC GTTTGGCACT AACGTGGGAG AAACTGCCAA GCAAGTTCAA GAAGTTCTAT
    GCGGAGTTTG AAAGCTTAAT GGATCCTTCG AGAAACCACA GGGCCTACCG GCTGACCATA
    GCTAAGCTGG ACCCCCCTCT CATCCCCTTC ATGCCTTTGC TCATTAAAGA TATGACATTT
    ACTCATGAGG GGAACAAGAC ATTCATTGAC AATCTAGTAA ACTTTGAAAA AATGCGCATG
    ATTGCAAATA CTGCCAGAAC AGTGAGATAC TGCAGGAGCC AACCCTTCAA TCCTGATGCC
    GCTCAAGCTA ATAAGAACCA TCAGGATGTC CGGAGTTATG TACGGCAATT AAATGTGATT
    GACAACCAGA GAACTTTATC ACAGATGTCA CACAGATTAG AGCCTCGTCG ACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023477774.1
    CDS3647..6223
    Translation

    Target ORF information:

    RefSeq Version XM_023622006.1
    Organism Equus caballus(horse)
    Definition Equus caballus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023622006.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGGAC TTCTGGCTCC TCCTTATGGT GTTATGGAAA CGGGCTCTAA CAATGACAGG 
    ATTCCTGACA AGGAGAATAC ACCTCTCATT GAACCTCACG TTCCTCTTCG TCCTGCTAAC
    ACCATTACCA AGGTCCCTTC AGAGAAGATC CTCAGGGCTG GAAAAATTTT ACGAAACGCC
    ATTCTCTCTC GAGCACCTCA CATGATTAGA GATAGGAAAT ACCACCTAAA GACGTACAGA
    CAATGCTGTG TGGGAACTGA GCTGGTGGAC TGGATGCTGC AGCAGACTCC GTGTGCCCAC
    TCCCGGACTC AAGCTGTTGG GATGTGGCAA GTGCTGGTGG AAGATGGTGT TCTCAACCAT
    GTGGACCAGG AGCATCATTT CCAAGACAAA TATTTATTTT ATCGATTTCT GGATGACGAG
    CACGAGGATG CCCCTTTGCC TACTGAGGAG GAGAAGAAGG AGTGTGATGA GGAGCTCCAG
    GACACCATGC TGCTGCTGTC ACAGATGGGC CCCGATGCCC ACATGAGGAT GATCCTCCGC
    AAACCACCTG GCCAGAGGAC TGTGGATGAC CTAGAAATTA TCTACGAAGA GCTCCTTCAC
    ATTAAAGCCT TATCCCACCT TTCCACCACT GTGAAACGGG AGTTGGCAGG TGTTCTCATT
    TTTGAGTCGC ACGCCAAAGG AGGAACCGTG TTGTTCAACC AGGGGGAAGA AGGTACCTCC
    TGGTACATTA TTCTAAAAGG ATCGGTGAAT GTAGTCATTT ATGGCAAGGG TGTGGTCTGC
    ACCCTGCATG AGGGAGATGA CTTTGGCAAG CTAGCACTAG TGAACGACGC CCCGAGAGCT
    GCCTCCATCG TCTTGCGAGA AGATAATTGC CATTTCTTAA GAGTGGACAA GGAGGATTTC
    AACCGAATCC TGAGGGACGT TGAGGCGAAT ACAGTCAGAC TTAAAGAACA TGACCAAGAT
    GTCTTGGTGC TGGAGAAGGT CCCAGCAGGG AACAGGGCTT CTAATCAAGG AAACTCACAG
    CCTCAGCAAA AGTATACTGT GATGTCAGGA ACACCTGAAA AAATTTTAGA GCATTTTCTA
    GAAACAATAC GCCTTGAGCC AGCTTTAAAT GAAGCAACAG ATTCTGTTTT AAACGACTTT
    GTTATGATGC ACTGTGTTTT TATGCCAAAT ATCCAGCTTT GTCCAGCCCT GGTGGCCCAT
    TACCATGCAC AGCCCTCACA AGGTACAGAA CAGGAGAAAA TGGATTATGC CCTCAACAAT
    AAGAGACGAG TCATCCGGCT GGTTTTACAG TGGGCCGCCA TGTATGGAGA CGTCCTACAA
    GAGGATGACG TAGCTATGGC TTTCCTGGAG GAGTTTTATG TGTCTGTGTC AGATGATGCC
    CGGATGATTG CTGCCCTCAA GGAGCAACTG CCCGAGCTGG AGAAGATCGT CAAGCAAATT
    TCAGAAGATG CAAAAGCTCC ACAAAAGAAG CACAAAGTTC TTTTGCAACA GTTCAACACA
    GGCGATGAGA GAGCCCAGAA GCGCCAGCCT ATCCGTGGCT CTGATGAAGT TCTGTTTAAG
    GTCTATTGCA TGGACCACAC CTACACAACC ATTCGGGTGC CGGTGGCCGC CTCAGTGAAG
    GAAGTCATCA GCGCAGTTGC CGACAAACTG GGCTCCGGAG AAGGCCTGAT CATAGTCAAG
    ATGAGTTCCG GAGGAGAAAA GGTGGTGCTC AAACCTAATG ATGTTTCAGT ATTTACAACG
    CTCACCATTA ATGGACGCCT GTTTGCTTGC CCGCGAGAGC AATTCGACTC ACTGACTCCC
    CTGCCAGAAC AGGAAGGCCC AACTGTTGGA ACAGTGGGAA CTTTTGAACT GATGAGCTCC
    AAAGATTTAG CATACCAGAT GACAATTTAT GATTGGGAAC TCTTCAACTG TGTGCATGAG
    CTGGAGCTAA TCTATCACAC ATTTGGAAGG CATAATTTTA AAAAGACCAC AGCAAACTTG
    GATTTGTTCC TGAGAAGATT TAATGAAATT CAGTTTTGGG TTGTCACCGA GATTTGCCTT
    TGTCCCCAGC CCAGCAAGCG TGTTCAGCTC TTAAAAAAAT TTATTAAGAT CGCAGCCCAC
    TGTAAGGAGT ACAAAAATCT GAATTCCTTT TTTGCCATCG TCATGGGACT AAGTAATGTT
    GCCGTGAGCC GTTTGGCACT AACGTGGGAG AAACTGCCAA GCAAGTTCAA GAAGTTCTAT
    GCGGAGTTTG AAAGCTTAAT GGATCCTTCG AGAAACCACA GGGCCTACCG GCTGACCATA
    GCTAAGCTGG ACCCCCCTCT CATCCCCTTC ATGCCTTTGC TCATTAAAGA TATGACATTT
    ACTCATGAGG GGAACAAGAC ATTCATTGAC AATCTAGTAA ACTTTGAAAA AATGCGCATG
    ATTGCAAATA CTGCCAGAAC AGTGAGATAC TGCAGGAGCC AACCCTTCAA TCCTGATGCC
    GCTCAAGCTA ATAAGAACCA TCAGGATGTC CGGAGTTATG TACGGCAATT AAATGTGATT
    GACAACCAGA GAACTTTATC ACAGATGTCA CACAGATTAG AGCCTCGTCG ACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb52134
    Clone ID Related Accession (Same CDS sequence) XM_023622005.1 , XM_023622006.1 , XM_023622004.1
    Accession Version XM_023622004.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product rap guanine nucleotide exchange factor 4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGGAC TTCTGGCTCC TCCTTATGGT GTTATGGAAA CGGGCTCTAA CAATGACAGG 
    ATTCCTGACA AGGAGAATAC ACCTCTCATT GAACCTCACG TTCCTCTTCG TCCTGCTAAC
    ACCATTACCA AGGTCCCTTC AGAGAAGATC CTCAGGGCTG GAAAAATTTT ACGAAACGCC
    ATTCTCTCTC GAGCACCTCA CATGATTAGA GATAGGAAAT ACCACCTAAA GACGTACAGA
    CAATGCTGTG TGGGAACTGA GCTGGTGGAC TGGATGCTGC AGCAGACTCC GTGTGCCCAC
    TCCCGGACTC AAGCTGTTGG GATGTGGCAA GTGCTGGTGG AAGATGGTGT TCTCAACCAT
    GTGGACCAGG AGCATCATTT CCAAGACAAA TATTTATTTT ATCGATTTCT GGATGACGAG
    CACGAGGATG CCCCTTTGCC TACTGAGGAG GAGAAGAAGG AGTGTGATGA GGAGCTCCAG
    GACACCATGC TGCTGCTGTC ACAGATGGGC CCCGATGCCC ACATGAGGAT GATCCTCCGC
    AAACCACCTG GCCAGAGGAC TGTGGATGAC CTAGAAATTA TCTACGAAGA GCTCCTTCAC
    ATTAAAGCCT TATCCCACCT TTCCACCACT GTGAAACGGG AGTTGGCAGG TGTTCTCATT
    TTTGAGTCGC ACGCCAAAGG AGGAACCGTG TTGTTCAACC AGGGGGAAGA AGGTACCTCC
    TGGTACATTA TTCTAAAAGG ATCGGTGAAT GTAGTCATTT ATGGCAAGGG TGTGGTCTGC
    ACCCTGCATG AGGGAGATGA CTTTGGCAAG CTAGCACTAG TGAACGACGC CCCGAGAGCT
    GCCTCCATCG TCTTGCGAGA AGATAATTGC CATTTCTTAA GAGTGGACAA GGAGGATTTC
    AACCGAATCC TGAGGGACGT TGAGGCGAAT ACAGTCAGAC TTAAAGAACA TGACCAAGAT
    GTCTTGGTGC TGGAGAAGGT CCCAGCAGGG AACAGGGCTT CTAATCAAGG AAACTCACAG
    CCTCAGCAAA AGTATACTGT GATGTCAGGA ACACCTGAAA AAATTTTAGA GCATTTTCTA
    GAAACAATAC GCCTTGAGCC AGCTTTAAAT GAAGCAACAG ATTCTGTTTT AAACGACTTT
    GTTATGATGC ACTGTGTTTT TATGCCAAAT ATCCAGCTTT GTCCAGCCCT GGTGGCCCAT
    TACCATGCAC AGCCCTCACA AGGTACAGAA CAGGAGAAAA TGGATTATGC CCTCAACAAT
    AAGAGACGAG TCATCCGGCT GGTTTTACAG TGGGCCGCCA TGTATGGAGA CGTCCTACAA
    GAGGATGACG TAGCTATGGC TTTCCTGGAG GAGTTTTATG TGTCTGTGTC AGATGATGCC
    CGGATGATTG CTGCCCTCAA GGAGCAACTG CCCGAGCTGG AGAAGATCGT CAAGCAAATT
    TCAGAAGATG CAAAAGCTCC ACAAAAGAAG CACAAAGTTC TTTTGCAACA GTTCAACACA
    GGCGATGAGA GAGCCCAGAA GCGCCAGCCT ATCCGTGGCT CTGATGAAGT TCTGTTTAAG
    GTCTATTGCA TGGACCACAC CTACACAACC ATTCGGGTGC CGGTGGCCGC CTCAGTGAAG
    GAAGTCATCA GCGCAGTTGC CGACAAACTG GGCTCCGGAG AAGGCCTGAT CATAGTCAAG
    ATGAGTTCCG GAGGAGAAAA GGTGGTGCTC AAACCTAATG ATGTTTCAGT ATTTACAACG
    CTCACCATTA ATGGACGCCT GTTTGCTTGC CCGCGAGAGC AATTCGACTC ACTGACTCCC
    CTGCCAGAAC AGGAAGGCCC AACTGTTGGA ACAGTGGGAA CTTTTGAACT GATGAGCTCC
    AAAGATTTAG CATACCAGAT GACAATTTAT GATTGGGAAC TCTTCAACTG TGTGCATGAG
    CTGGAGCTAA TCTATCACAC ATTTGGAAGG CATAATTTTA AAAAGACCAC AGCAAACTTG
    GATTTGTTCC TGAGAAGATT TAATGAAATT CAGTTTTGGG TTGTCACCGA GATTTGCCTT
    TGTCCCCAGC CCAGCAAGCG TGTTCAGCTC TTAAAAAAAT TTATTAAGAT CGCAGCCCAC
    TGTAAGGAGT ACAAAAATCT GAATTCCTTT TTTGCCATCG TCATGGGACT AAGTAATGTT
    GCCGTGAGCC GTTTGGCACT AACGTGGGAG AAACTGCCAA GCAAGTTCAA GAAGTTCTAT
    GCGGAGTTTG AAAGCTTAAT GGATCCTTCG AGAAACCACA GGGCCTACCG GCTGACCATA
    GCTAAGCTGG ACCCCCCTCT CATCCCCTTC ATGCCTTTGC TCATTAAAGA TATGACATTT
    ACTCATGAGG GGAACAAGAC ATTCATTGAC AATCTAGTAA ACTTTGAAAA AATGCGCATG
    ATTGCAAATA CTGCCAGAAC AGTGAGATAC TGCAGGAGCC AACCCTTCAA TCCTGATGCC
    GCTCAAGCTA ATAAGAACCA TCAGGATGTC CGGAGTTATG TACGGCAATT AAATGTGATT
    GACAACCAGA GAACTTTATC ACAGATGTCA CACAGATTAG AGCCTCGTCG ACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023477772.1
    CDS5213..7789
    Translation

    Target ORF information:

    RefSeq Version XM_023622004.1
    Organism Equus caballus(horse)
    Definition Equus caballus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023622004.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGGAC TTCTGGCTCC TCCTTATGGT GTTATGGAAA CGGGCTCTAA CAATGACAGG 
    ATTCCTGACA AGGAGAATAC ACCTCTCATT GAACCTCACG TTCCTCTTCG TCCTGCTAAC
    ACCATTACCA AGGTCCCTTC AGAGAAGATC CTCAGGGCTG GAAAAATTTT ACGAAACGCC
    ATTCTCTCTC GAGCACCTCA CATGATTAGA GATAGGAAAT ACCACCTAAA GACGTACAGA
    CAATGCTGTG TGGGAACTGA GCTGGTGGAC TGGATGCTGC AGCAGACTCC GTGTGCCCAC
    TCCCGGACTC AAGCTGTTGG GATGTGGCAA GTGCTGGTGG AAGATGGTGT TCTCAACCAT
    GTGGACCAGG AGCATCATTT CCAAGACAAA TATTTATTTT ATCGATTTCT GGATGACGAG
    CACGAGGATG CCCCTTTGCC TACTGAGGAG GAGAAGAAGG AGTGTGATGA GGAGCTCCAG
    GACACCATGC TGCTGCTGTC ACAGATGGGC CCCGATGCCC ACATGAGGAT GATCCTCCGC
    AAACCACCTG GCCAGAGGAC TGTGGATGAC CTAGAAATTA TCTACGAAGA GCTCCTTCAC
    ATTAAAGCCT TATCCCACCT TTCCACCACT GTGAAACGGG AGTTGGCAGG TGTTCTCATT
    TTTGAGTCGC ACGCCAAAGG AGGAACCGTG TTGTTCAACC AGGGGGAAGA AGGTACCTCC
    TGGTACATTA TTCTAAAAGG ATCGGTGAAT GTAGTCATTT ATGGCAAGGG TGTGGTCTGC
    ACCCTGCATG AGGGAGATGA CTTTGGCAAG CTAGCACTAG TGAACGACGC CCCGAGAGCT
    GCCTCCATCG TCTTGCGAGA AGATAATTGC CATTTCTTAA GAGTGGACAA GGAGGATTTC
    AACCGAATCC TGAGGGACGT TGAGGCGAAT ACAGTCAGAC TTAAAGAACA TGACCAAGAT
    GTCTTGGTGC TGGAGAAGGT CCCAGCAGGG AACAGGGCTT CTAATCAAGG AAACTCACAG
    CCTCAGCAAA AGTATACTGT GATGTCAGGA ACACCTGAAA AAATTTTAGA GCATTTTCTA
    GAAACAATAC GCCTTGAGCC AGCTTTAAAT GAAGCAACAG ATTCTGTTTT AAACGACTTT
    GTTATGATGC ACTGTGTTTT TATGCCAAAT ATCCAGCTTT GTCCAGCCCT GGTGGCCCAT
    TACCATGCAC AGCCCTCACA AGGTACAGAA CAGGAGAAAA TGGATTATGC CCTCAACAAT
    AAGAGACGAG TCATCCGGCT GGTTTTACAG TGGGCCGCCA TGTATGGAGA CGTCCTACAA
    GAGGATGACG TAGCTATGGC TTTCCTGGAG GAGTTTTATG TGTCTGTGTC AGATGATGCC
    CGGATGATTG CTGCCCTCAA GGAGCAACTG CCCGAGCTGG AGAAGATCGT CAAGCAAATT
    TCAGAAGATG CAAAAGCTCC ACAAAAGAAG CACAAAGTTC TTTTGCAACA GTTCAACACA
    GGCGATGAGA GAGCCCAGAA GCGCCAGCCT ATCCGTGGCT CTGATGAAGT TCTGTTTAAG
    GTCTATTGCA TGGACCACAC CTACACAACC ATTCGGGTGC CGGTGGCCGC CTCAGTGAAG
    GAAGTCATCA GCGCAGTTGC CGACAAACTG GGCTCCGGAG AAGGCCTGAT CATAGTCAAG
    ATGAGTTCCG GAGGAGAAAA GGTGGTGCTC AAACCTAATG ATGTTTCAGT ATTTACAACG
    CTCACCATTA ATGGACGCCT GTTTGCTTGC CCGCGAGAGC AATTCGACTC ACTGACTCCC
    CTGCCAGAAC AGGAAGGCCC AACTGTTGGA ACAGTGGGAA CTTTTGAACT GATGAGCTCC
    AAAGATTTAG CATACCAGAT GACAATTTAT GATTGGGAAC TCTTCAACTG TGTGCATGAG
    CTGGAGCTAA TCTATCACAC ATTTGGAAGG CATAATTTTA AAAAGACCAC AGCAAACTTG
    GATTTGTTCC TGAGAAGATT TAATGAAATT CAGTTTTGGG TTGTCACCGA GATTTGCCTT
    TGTCCCCAGC CCAGCAAGCG TGTTCAGCTC TTAAAAAAAT TTATTAAGAT CGCAGCCCAC
    TGTAAGGAGT ACAAAAATCT GAATTCCTTT TTTGCCATCG TCATGGGACT AAGTAATGTT
    GCCGTGAGCC GTTTGGCACT AACGTGGGAG AAACTGCCAA GCAAGTTCAA GAAGTTCTAT
    GCGGAGTTTG AAAGCTTAAT GGATCCTTCG AGAAACCACA GGGCCTACCG GCTGACCATA
    GCTAAGCTGG ACCCCCCTCT CATCCCCTTC ATGCCTTTGC TCATTAAAGA TATGACATTT
    ACTCATGAGG GGAACAAGAC ATTCATTGAC AATCTAGTAA ACTTTGAAAA AATGCGCATG
    ATTGCAAATA CTGCCAGAAC AGTGAGATAC TGCAGGAGCC AACCCTTCAA TCCTGATGCC
    GCTCAAGCTA ATAAGAACCA TCAGGATGTC CGGAGTTATG TACGGCAATT AAATGTGATT
    GACAACCAGA GAACTTTATC ACAGATGTCA CACAGATTAG AGCCTCGTCG ACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb52127
    Clone ID Related Accession (Same CDS sequence) XM_023621997.1
    Accession Version XM_023621997.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3060bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product rap guanine nucleotide exchange factor 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTGCTTCT CTCTGCTGAT ACTCGTGGGC GTGTATCCTG TGCCTCAGGT GGGTTGGCAT 
    GGATGGAAGA TTGAGACTCA CCGTTCTAGA CCGCTGGAAC GATCCAGTGA AGATGTAGAT
    ATAATCTTCA CACGGCTGAA AGAAGTTAAA GCTTTTGAGA AATTTCACCC AAATCTCCTT
    CATCAGATTT GTTTGTGTGG TTATTATGAG AATCTGGAAA AAGGAATAAC ATTATTTCGC
    CAGGGTGATA TTGGAACAAA CTGGTATGCT GTCCTGGCAG GGTCTTTGGA TGTTAAAGTA
    TCTGAAACCA GCAGTCACCA GGATGCAGTG ACCATCTGTA CTCTGGGAAT TGGGACAGCC
    TTTGGAGAGT CCATTCTGGA CAACACACCC CGCCATGCGA CCATCGTTAC CAGGGAGAGC
    AGCGAACTGC TCCGCATCGA GCAGAAGGAC TTCAAGGCAC TATGGGAGAA ATATCGACAG
    TACATGGCAG GACTTCTGGC TCCTCCTTAT GGTGTTATGG AAACGGGCTC TAACAATGAC
    AGGATTCCTG ACAAGGAGAA TACACCTCTC ATTGAACCTC ACGTTCCTCT TCGTCCTGCT
    AACACCATTA CCAAGGTCCC TTCAGAGAAG ATCCTCAGGG CTGGAAAAAT TTTACGAAAC
    GCCATTCTCT CTCGAGCACC TCACATGATT AGAGATAGGA AATACCACCT AAAGACGTAC
    AGACAATGCT GTGTGGGAAC TGAGCTGGTG GACTGGATGC TGCAGCAGAC TCCGTGTGCC
    CACTCCCGGA CTCAAGCTGT TGGGATGTGG CAAGTGCTGG TGGAAGATGG TGTTCTCAAC
    CATGTGGACC AGGAGCATCA TTTCCAAGAC AAATATTTAT TTTATCGATT TCTGGATGAC
    GAGCACGAGG ATGCCCCTTT GCCTACTGAG GAGGAGAAGA AGGAGTGTGA TGAGGAGCTC
    CAGGACACCA TGCTGCTGCT GTCACAGATG GGCCCCGATG CCCACATGAG GATGATCCTC
    CGCAAACCAC CTGGCCAGAG GACTGTGGAT GACCTAGAAA TTATCTACGA AGAGCTCCTT
    CACATTAAAG CCTTATCCCA CCTTTCCACC ACTGTGAAAC GGGAGTTGGC AGGTGTTCTC
    ATTTTTGAGT CGCACGCCAA AGGAGGAACC GTGTTGTTCA ACCAGGGGGA AGAAGGTACC
    TCCTGGTACA TTATTCTAAA AGGATCGGTG AATGTAGTCA TTTATGGCAA GGGTGTGGTC
    TGCACCCTGC ATGAGGGAGA TGACTTTGGC AAGCTAGCAC TAGTGAACGA CGCCCCGAGA
    GCTGCCTCCA TCGTCTTGCG AGAAGATAAT TGCCATTTCT TAAGAGTGGA CAAGGAGGAT
    TTCAACCGAA TCCTGAGGGA CGTTGAGGCG AATACAGTCA GACTTAAAGA ACATGACCAA
    GATGTCTTGG TGCTGGAGAA GGTCCCAGCA GGGAACAGGG CTTCTAATCA AGGAAACTCA
    CAGCCTCAGC AAAAGTATAC TGTGATGTCA GGAACACCTG AAAAAATTTT AGAGCATTTT
    CTAGAAACAA TACGCCTTGA GCCAGCTTTA AATGAAGCAA CAGATTCTGT TTTAAACGAC
    TTTGTTATGA TGCACTGTGT TTTTATGCCA AATATCCAGC TTTGTCCAGC CCTGGTGGCC
    CATTACCATG CACAGCCCTC ACAAGGTACA GAACAGGAGA AAATGGATTA TGCCCTCAAC
    AATAAGAGAC GAGTCATCCG GCTGGTTTTA CAGTGGGCCG CCATGTATGG AGACGTCCTA
    CAAGAGGATG ACGTAGCTAT GGCTTTCCTG GAGGAGTTTT ATGTGTCTGT GTCAGATGAT
    GCCCGGATGA TTGCTGCCCT CAAGGAGCAA CTGCCCGAGC TGGAGAAGAT CGTCAAGCAA
    ATTTCAGAAG ATGCAAAAGC TCCACAAAAG AAGCACAAAG TTCTTTTGCA ACAGTTCAAC
    ACAGGCGATG AGAGAGCCCA GAAGCGCCAG CCTATCCGTG GCTCTGATGA AGTTCTGTTT
    AAGGTCTATT GCATGGACCA CACCTACACA ACCATTCGGG TGCCGGTGGC CGCCTCAGTG
    AAGGAAGTCA TCAGCGCAGT TGCCGACAAA CTGGGCTCCG GAGAAGGCCT GATCATAGTC
    AAGATGAGTT CCGGAGGAGA AAAGGTGGTG CTCAAACCTA ATGATGTTTC AGTATTTACA
    ACGCTCACCA TTAATGGACG CCTGTTTGCT TGCCCGCGAG AGCAATTCGA CTCACTGACT
    CCCCTGCCAG AACAGGAAGG CCCAACTGTT GGAACAGTGG GAACTTTTGA ACTGATGAGC
    TCCAAAGATT TAGCATACCA GATGACAATT TATGATTGGG AACTCTTCAA CTGTGTGCAT
    GAGCTGGAGC TAATCTATCA CACATTTGGA AGGCATAATT TTAAAAAGAC CACAGCAAAC
    TTGGATTTGT TCCTGAGAAG ATTTAATGAA ATTCAGTTTT GGGTTGTCAC CGAGATTTGC
    CTTTGTCCCC AGCCCAGCAA GCGTGTTCAG CTCTTAAAAA AATTTATTAA GATCGCAGCC
    CACTGTAAGG AGTACAAAAA TCTGAATTCC TTTTTTGCCA TCGTCATGGG ACTAAGTAAT
    GTTGCCGTGA GCCGTTTGGC ACTAACGTGG GAGAAACTGC CAAGCAAGTT CAAGAAGTTC
    TATGCGGAGT TTGAAAGCTT AATGGATCCT TCGAGAAACC ACAGGGCCTA CCGGCTGACC
    ATAGCTAAGC TGGACCCCCC TCTCATCCCC TTCATGCCTT TGCTCATTAA AGATATGACA
    TTTACTCATG AGGGGAACAA GACATTCATT GACAATCTAG TAAACTTTGA AAAAATGCGC
    ATGATTGCAA ATACTGCCAG AACAGTGAGA TACTGCAGGA GCCAACCCTT CAATCCTGAT
    GCCGCTCAAG CTAATAAGAA CCATCAGGAT GTCCGGAGTT ATGTACGGCA ATTAAATGTG
    ATTGACAACC AGAGAACTTT ATCACAGATG TCACACAGAT TAGAGCCTCG TCGACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023477765.1
    CDS97..3156
    Translation

    Target ORF information:

    RefSeq Version XM_023621997.1
    Organism Equus caballus(horse)
    Definition Equus caballus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023621997.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTGCTTCT CTCTGCTGAT ACTCGTGGGC GTGTATCCTG TGCCTCAGGT GGGTTGGCAT 
    GGATGGAAGA TTGAGACTCA CCGTTCTAGA CCGCTGGAAC GATCCAGTGA AGATGTAGAT
    ATAATCTTCA CACGGCTGAA AGAAGTTAAA GCTTTTGAGA AATTTCACCC AAATCTCCTT
    CATCAGATTT GTTTGTGTGG TTATTATGAG AATCTGGAAA AAGGAATAAC ATTATTTCGC
    CAGGGTGATA TTGGAACAAA CTGGTATGCT GTCCTGGCAG GGTCTTTGGA TGTTAAAGTA
    TCTGAAACCA GCAGTCACCA GGATGCAGTG ACCATCTGTA CTCTGGGAAT TGGGACAGCC
    TTTGGAGAGT CCATTCTGGA CAACACACCC CGCCATGCGA CCATCGTTAC CAGGGAGAGC
    AGCGAACTGC TCCGCATCGA GCAGAAGGAC TTCAAGGCAC TATGGGAGAA ATATCGACAG
    TACATGGCAG GACTTCTGGC TCCTCCTTAT GGTGTTATGG AAACGGGCTC TAACAATGAC
    AGGATTCCTG ACAAGGAGAA TACACCTCTC ATTGAACCTC ACGTTCCTCT TCGTCCTGCT
    AACACCATTA CCAAGGTCCC TTCAGAGAAG ATCCTCAGGG CTGGAAAAAT TTTACGAAAC
    GCCATTCTCT CTCGAGCACC TCACATGATT AGAGATAGGA AATACCACCT AAAGACGTAC
    AGACAATGCT GTGTGGGAAC TGAGCTGGTG GACTGGATGC TGCAGCAGAC TCCGTGTGCC
    CACTCCCGGA CTCAAGCTGT TGGGATGTGG CAAGTGCTGG TGGAAGATGG TGTTCTCAAC
    CATGTGGACC AGGAGCATCA TTTCCAAGAC AAATATTTAT TTTATCGATT TCTGGATGAC
    GAGCACGAGG ATGCCCCTTT GCCTACTGAG GAGGAGAAGA AGGAGTGTGA TGAGGAGCTC
    CAGGACACCA TGCTGCTGCT GTCACAGATG GGCCCCGATG CCCACATGAG GATGATCCTC
    CGCAAACCAC CTGGCCAGAG GACTGTGGAT GACCTAGAAA TTATCTACGA AGAGCTCCTT
    CACATTAAAG CCTTATCCCA CCTTTCCACC ACTGTGAAAC GGGAGTTGGC AGGTGTTCTC
    ATTTTTGAGT CGCACGCCAA AGGAGGAACC GTGTTGTTCA ACCAGGGGGA AGAAGGTACC
    TCCTGGTACA TTATTCTAAA AGGATCGGTG AATGTAGTCA TTTATGGCAA GGGTGTGGTC
    TGCACCCTGC ATGAGGGAGA TGACTTTGGC AAGCTAGCAC TAGTGAACGA CGCCCCGAGA
    GCTGCCTCCA TCGTCTTGCG AGAAGATAAT TGCCATTTCT TAAGAGTGGA CAAGGAGGAT
    TTCAACCGAA TCCTGAGGGA CGTTGAGGCG AATACAGTCA GACTTAAAGA ACATGACCAA
    GATGTCTTGG TGCTGGAGAA GGTCCCAGCA GGGAACAGGG CTTCTAATCA AGGAAACTCA
    CAGCCTCAGC AAAAGTATAC TGTGATGTCA GGAACACCTG AAAAAATTTT AGAGCATTTT
    CTAGAAACAA TACGCCTTGA GCCAGCTTTA AATGAAGCAA CAGATTCTGT TTTAAACGAC
    TTTGTTATGA TGCACTGTGT TTTTATGCCA AATATCCAGC TTTGTCCAGC CCTGGTGGCC
    CATTACCATG CACAGCCCTC ACAAGGTACA GAACAGGAGA AAATGGATTA TGCCCTCAAC
    AATAAGAGAC GAGTCATCCG GCTGGTTTTA CAGTGGGCCG CCATGTATGG AGACGTCCTA
    CAAGAGGATG ACGTAGCTAT GGCTTTCCTG GAGGAGTTTT ATGTGTCTGT GTCAGATGAT
    GCCCGGATGA TTGCTGCCCT CAAGGAGCAA CTGCCCGAGC TGGAGAAGAT CGTCAAGCAA
    ATTTCAGAAG ATGCAAAAGC TCCACAAAAG AAGCACAAAG TTCTTTTGCA ACAGTTCAAC
    ACAGGCGATG AGAGAGCCCA GAAGCGCCAG CCTATCCGTG GCTCTGATGA AGTTCTGTTT
    AAGGTCTATT GCATGGACCA CACCTACACA ACCATTCGGG TGCCGGTGGC CGCCTCAGTG
    AAGGAAGTCA TCAGCGCAGT TGCCGACAAA CTGGGCTCCG GAGAAGGCCT GATCATAGTC
    AAGATGAGTT CCGGAGGAGA AAAGGTGGTG CTCAAACCTA ATGATGTTTC AGTATTTACA
    ACGCTCACCA TTAATGGACG CCTGTTTGCT TGCCCGCGAG AGCAATTCGA CTCACTGACT
    CCCCTGCCAG AACAGGAAGG CCCAACTGTT GGAACAGTGG GAACTTTTGA ACTGATGAGC
    TCCAAAGATT TAGCATACCA GATGACAATT TATGATTGGG AACTCTTCAA CTGTGTGCAT
    GAGCTGGAGC TAATCTATCA CACATTTGGA AGGCATAATT TTAAAAAGAC CACAGCAAAC
    TTGGATTTGT TCCTGAGAAG ATTTAATGAA ATTCAGTTTT GGGTTGTCAC CGAGATTTGC
    CTTTGTCCCC AGCCCAGCAA GCGTGTTCAG CTCTTAAAAA AATTTATTAA GATCGCAGCC
    CACTGTAAGG AGTACAAAAA TCTGAATTCC TTTTTTGCCA TCGTCATGGG ACTAAGTAAT
    GTTGCCGTGA GCCGTTTGGC ACTAACGTGG GAGAAACTGC CAAGCAAGTT CAAGAAGTTC
    TATGCGGAGT TTGAAAGCTT AATGGATCCT TCGAGAAACC ACAGGGCCTA CCGGCTGACC
    ATAGCTAAGC TGGACCCCCC TCTCATCCCC TTCATGCCTT TGCTCATTAA AGATATGACA
    TTTACTCATG AGGGGAACAA GACATTCATT GACAATCTAG TAAACTTTGA AAAAATGCGC
    ATGATTGCAA ATACTGCCAG AACAGTGAGA TACTGCAGGA GCCAACCCTT CAATCCTGAT
    GCCGCTCAAG CTAATAAGAA CCATCAGGAT GTCCGGAGTT ATGTACGGCA ATTAAATGTG
    ATTGACAACC AGAGAACTTT ATCACAGATG TCACACAGAT TAGAGCCTCG TCGACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb52128
    Clone ID Related Accession (Same CDS sequence) XM_023621998.1
    Accession Version XM_023621998.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product rap guanine nucleotide exchange factor 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCGTCGGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCGC TGGAACGATC CAGTGAAGAT GTAGATATAA TCTTCACACG GCTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGTTT GTGTGGTTAT
    TATGAGAATC TGGAAAAAGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AAACCAGCAG TCACCAGGAT
    GCAGTGACCA TCTGTACTCT GGGAATTGGG ACAGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCGACCAT CGTTACCAGG GAGAGCAGCG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTACA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAATACA
    CCTCTCATTG AACCTCACGT TCCTCTTCGT CCTGCTAACA CCATTACCAA GGTCCCTTCA
    GAGAAGATCC TCAGGGCTGG AAAAATTTTA CGAAACGCCA TTCTCTCTCG AGCACCTCAC
    ATGATTAGAG ATAGGAAATA CCACCTAAAG ACGTACAGAC AATGCTGTGT GGGAACTGAG
    CTGGTGGACT GGATGCTGCA GCAGACTCCG TGTGCCCACT CCCGGACTCA AGCTGTTGGG
    ATGTGGCAAG TGCTGGTGGA AGATGGTGTT CTCAACCATG TGGACCAGGA GCATCATTTC
    CAAGACAAAT ATTTATTTTA TCGATTTCTG GATGACGAGC ACGAGGATGC CCCTTTGCCT
    ACTGAGGAGG AGAAGAAGGA GTGTGATGAG GAGCTCCAGG ACACCATGCT GCTGCTGTCA
    CAGATGGGCC CCGATGCCCA CATGAGGATG ATCCTCCGCA AACCACCTGG CCAGAGGACT
    GTGGATGACC TAGAAATTAT CTACGAAGAG CTCCTTCACA TTAAAGCCTT ATCCCACCTT
    TCCACCACTG TGAAACGGGA GTTGGCAGGT GTTCTCATTT TTGAGTCGCA CGCCAAAGGA
    GGAACCGTGT TGTTCAACCA GGGGGAAGAA GGTACCTCCT GGTACATTAT TCTAAAAGGA
    TCGGTGAATG TAGTCATTTA TGGCAAGGGT GTGGTCTGCA CCCTGCATGA GGGAGATGAC
    TTTGGCAAGC TAGCACTAGT GAACGACGCC CCGAGAGCTG CCTCCATCGT CTTGCGAGAA
    GATAATTGCC ATTTCTTAAG AGTGGACAAG GAGGATTTCA ACCGAATCCT GAGGGACGTT
    GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTGCT GGAGAAGGTC
    CCAGCAGGGA ACAGGGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG
    ATGTCAGGAA CACCTGAAAA AATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGCCA
    GCTTTAAATG AAGCAACAGA TTCTGTTTTA AACGACTTTG TTATGATGCA CTGTGTTTTT
    ATGCCAAATA TCCAGCTTTG TCCAGCCCTG GTGGCCCATT ACCATGCACA GCCCTCACAA
    GGTACAGAAC AGGAGAAAAT GGATTATGCC CTCAACAATA AGAGACGAGT CATCCGGCTG
    GTTTTACAGT GGGCCGCCAT GTATGGAGAC GTCCTACAAG AGGATGACGT AGCTATGGCT
    TTCCTGGAGG AGTTTTATGT GTCTGTGTCA GATGATGCCC GGATGATTGC TGCCCTCAAG
    GAGCAACTGC CCGAGCTGGA GAAGATCGTC AAGCAAATTT CAGAAGATGC AAAAGCTCCA
    CAAAAGAAGC ACAAAGTTCT TTTGCAACAG TTCAACACAG GCGATGAGAG AGCCCAGAAG
    CGCCAGCCTA TCCGTGGCTC TGATGAAGTT CTGTTTAAGG TCTATTGCAT GGACCACACC
    TACACAACCA TTCGGGTGCC GGTGGCCGCC TCAGTGAAGG AAGTCATCAG CGCAGTTGCC
    GACAAACTGG GCTCCGGAGA AGGCCTGATC ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG
    GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACAACGC TCACCATTAA TGGACGCCTG
    TTTGCTTGCC CGCGAGAGCA ATTCGACTCA CTGACTCCCC TGCCAGAACA GGAAGGCCCA
    ACTGTTGGAA CAGTGGGAAC TTTTGAACTG ATGAGCTCCA AAGATTTAGC ATACCAGATG
    ACAATTTATG ATTGGGAACT CTTCAACTGT GTGCATGAGC TGGAGCTAAT CTATCACACA
    TTTGGAAGGC ATAATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGAAGATTT
    AATGAAATTC AGTTTTGGGT TGTCACCGAG ATTTGCCTTT GTCCCCAGCC CAGCAAGCGT
    GTTCAGCTCT TAAAAAAATT TATTAAGATC GCAGCCCACT GTAAGGAGTA CAAAAATCTG
    AATTCCTTTT TTGCCATCGT CATGGGACTA AGTAATGTTG CCGTGAGCCG TTTGGCACTA
    ACGTGGGAGA AACTGCCAAG CAAGTTCAAG AAGTTCTATG CGGAGTTTGA AAGCTTAATG
    GATCCTTCGA GAAACCACAG GGCCTACCGG CTGACCATAG CTAAGCTGGA CCCCCCTCTC
    ATCCCCTTCA TGCCTTTGCT CATTAAAGAT ATGACATTTA CTCATGAGGG GAACAAGACA
    TTCATTGACA ATCTAGTAAA CTTTGAAAAA ATGCGCATGA TTGCAAATAC TGCCAGAACA
    GTGAGATACT GCAGGAGCCA ACCCTTCAAT CCTGATGCCG CTCAAGCTAA TAAGAACCAT
    CAGGATGTCC GGAGTTATGT ACGGCAATTA AATGTGATTG ACAACCAGAG AACTTTATCA
    CAGATGTCAC ACAGATTAGA GCCTCGTCGA CCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023477766.1
    CDS111..3146
    Translation

    Target ORF information:

    RefSeq Version XM_023621998.1
    Organism Equus caballus(horse)
    Definition Equus caballus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023621998.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCGTCGGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCGC TGGAACGATC CAGTGAAGAT GTAGATATAA TCTTCACACG GCTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGTTT GTGTGGTTAT
    TATGAGAATC TGGAAAAAGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AAACCAGCAG TCACCAGGAT
    GCAGTGACCA TCTGTACTCT GGGAATTGGG ACAGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCGACCAT CGTTACCAGG GAGAGCAGCG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTACA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAATACA
    CCTCTCATTG AACCTCACGT TCCTCTTCGT CCTGCTAACA CCATTACCAA GGTCCCTTCA
    GAGAAGATCC TCAGGGCTGG AAAAATTTTA CGAAACGCCA TTCTCTCTCG AGCACCTCAC
    ATGATTAGAG ATAGGAAATA CCACCTAAAG ACGTACAGAC AATGCTGTGT GGGAACTGAG
    CTGGTGGACT GGATGCTGCA GCAGACTCCG TGTGCCCACT CCCGGACTCA AGCTGTTGGG
    ATGTGGCAAG TGCTGGTGGA AGATGGTGTT CTCAACCATG TGGACCAGGA GCATCATTTC
    CAAGACAAAT ATTTATTTTA TCGATTTCTG GATGACGAGC ACGAGGATGC CCCTTTGCCT
    ACTGAGGAGG AGAAGAAGGA GTGTGATGAG GAGCTCCAGG ACACCATGCT GCTGCTGTCA
    CAGATGGGCC CCGATGCCCA CATGAGGATG ATCCTCCGCA AACCACCTGG CCAGAGGACT
    GTGGATGACC TAGAAATTAT CTACGAAGAG CTCCTTCACA TTAAAGCCTT ATCCCACCTT
    TCCACCACTG TGAAACGGGA GTTGGCAGGT GTTCTCATTT TTGAGTCGCA CGCCAAAGGA
    GGAACCGTGT TGTTCAACCA GGGGGAAGAA GGTACCTCCT GGTACATTAT TCTAAAAGGA
    TCGGTGAATG TAGTCATTTA TGGCAAGGGT GTGGTCTGCA CCCTGCATGA GGGAGATGAC
    TTTGGCAAGC TAGCACTAGT GAACGACGCC CCGAGAGCTG CCTCCATCGT CTTGCGAGAA
    GATAATTGCC ATTTCTTAAG AGTGGACAAG GAGGATTTCA ACCGAATCCT GAGGGACGTT
    GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTGCT GGAGAAGGTC
    CCAGCAGGGA ACAGGGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG
    ATGTCAGGAA CACCTGAAAA AATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGCCA
    GCTTTAAATG AAGCAACAGA TTCTGTTTTA AACGACTTTG TTATGATGCA CTGTGTTTTT
    ATGCCAAATA TCCAGCTTTG TCCAGCCCTG GTGGCCCATT ACCATGCACA GCCCTCACAA
    GGTACAGAAC AGGAGAAAAT GGATTATGCC CTCAACAATA AGAGACGAGT CATCCGGCTG
    GTTTTACAGT GGGCCGCCAT GTATGGAGAC GTCCTACAAG AGGATGACGT AGCTATGGCT
    TTCCTGGAGG AGTTTTATGT GTCTGTGTCA GATGATGCCC GGATGATTGC TGCCCTCAAG
    GAGCAACTGC CCGAGCTGGA GAAGATCGTC AAGCAAATTT CAGAAGATGC AAAAGCTCCA
    CAAAAGAAGC ACAAAGTTCT TTTGCAACAG TTCAACACAG GCGATGAGAG AGCCCAGAAG
    CGCCAGCCTA TCCGTGGCTC TGATGAAGTT CTGTTTAAGG TCTATTGCAT GGACCACACC
    TACACAACCA TTCGGGTGCC GGTGGCCGCC TCAGTGAAGG AAGTCATCAG CGCAGTTGCC
    GACAAACTGG GCTCCGGAGA AGGCCTGATC ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG
    GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACAACGC TCACCATTAA TGGACGCCTG
    TTTGCTTGCC CGCGAGAGCA ATTCGACTCA CTGACTCCCC TGCCAGAACA GGAAGGCCCA
    ACTGTTGGAA CAGTGGGAAC TTTTGAACTG ATGAGCTCCA AAGATTTAGC ATACCAGATG
    ACAATTTATG ATTGGGAACT CTTCAACTGT GTGCATGAGC TGGAGCTAAT CTATCACACA
    TTTGGAAGGC ATAATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGAAGATTT
    AATGAAATTC AGTTTTGGGT TGTCACCGAG ATTTGCCTTT GTCCCCAGCC CAGCAAGCGT
    GTTCAGCTCT TAAAAAAATT TATTAAGATC GCAGCCCACT GTAAGGAGTA CAAAAATCTG
    AATTCCTTTT TTGCCATCGT CATGGGACTA AGTAATGTTG CCGTGAGCCG TTTGGCACTA
    ACGTGGGAGA AACTGCCAAG CAAGTTCAAG AAGTTCTATG CGGAGTTTGA AAGCTTAATG
    GATCCTTCGA GAAACCACAG GGCCTACCGG CTGACCATAG CTAAGCTGGA CCCCCCTCTC
    ATCCCCTTCA TGCCTTTGCT CATTAAAGAT ATGACATTTA CTCATGAGGG GAACAAGACA
    TTCATTGACA ATCTAGTAAA CTTTGAAAAA ATGCGCATGA TTGCAAATAC TGCCAGAACA
    GTGAGATACT GCAGGAGCCA ACCCTTCAAT CCTGATGCCG CTCAAGCTAA TAAGAACCAT
    CAGGATGTCC GGAGTTATGT ACGGCAATTA AATGTGATTG ACAACCAGAG AACTTTATCA
    CAGATGTCAC ACAGATTAGA GCCTCGTCGA CCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb52129
    Clone ID Related Accession (Same CDS sequence) XM_023621999.1
    Accession Version XM_023621999.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3006bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product rap guanine nucleotide exchange factor 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTGCTTCT CTCTGCTGAT ACTCGTGGGC GTGTATCCTG TGCCTCAGGT GGGTTGGCAT 
    GGATGGAAGA TTGAGACTCA CCGTTCTAGA CCGCTGGAAC GATCCAGTGA AGATGTAGAT
    ATAATCTTCA CACGGCTGAA AGAAGTTAAA GCTTTTGAGA AATTTCACCC AAATCTCCTT
    CATCAGATTT GTTTGTGTGG TTATTATGAG AATCTGGAAA AAGGAATAAC ATTATTTCGC
    CAGGGTGATA TTGGAACAAA CTGGTATGCT GTCCTGGCAG GGTCTTTGGA TGTTAAAGTA
    TCTGAAACCA GCAGTCACCA GGATGCAGTG ACCATCTGTA CTCTGGGAAT TGGGACAGCC
    TTTGGAGAGT CCATTCTGGA CAACACACCC CGCCATGCGA CCATCGTTAC CAGGGAGAGC
    AGCGAACTGC TCCGCATCGA GCAGAAGGAC TTCAAGGCAC TATGGGAGAA ATATCGACAG
    TACATGGCAG GACTTCTGGC TCCTCCTTAT GGTGTTATGG AAACGGGCTC TAACAATGAC
    AGGATTCCTG ACAAGGAGAA TGTCCCTTCA GAGAAGATCC TCAGGGCTGG AAAAATTTTA
    CGAAACGCCA TTCTCTCTCG AGCACCTCAC ATGATTAGAG ATAGGAAATA CCACCTAAAG
    ACGTACAGAC AATGCTGTGT GGGAACTGAG CTGGTGGACT GGATGCTGCA GCAGACTCCG
    TGTGCCCACT CCCGGACTCA AGCTGTTGGG ATGTGGCAAG TGCTGGTGGA AGATGGTGTT
    CTCAACCATG TGGACCAGGA GCATCATTTC CAAGACAAAT ATTTATTTTA TCGATTTCTG
    GATGACGAGC ACGAGGATGC CCCTTTGCCT ACTGAGGAGG AGAAGAAGGA GTGTGATGAG
    GAGCTCCAGG ACACCATGCT GCTGCTGTCA CAGATGGGCC CCGATGCCCA CATGAGGATG
    ATCCTCCGCA AACCACCTGG CCAGAGGACT GTGGATGACC TAGAAATTAT CTACGAAGAG
    CTCCTTCACA TTAAAGCCTT ATCCCACCTT TCCACCACTG TGAAACGGGA GTTGGCAGGT
    GTTCTCATTT TTGAGTCGCA CGCCAAAGGA GGAACCGTGT TGTTCAACCA GGGGGAAGAA
    GGTACCTCCT GGTACATTAT TCTAAAAGGA TCGGTGAATG TAGTCATTTA TGGCAAGGGT
    GTGGTCTGCA CCCTGCATGA GGGAGATGAC TTTGGCAAGC TAGCACTAGT GAACGACGCC
    CCGAGAGCTG CCTCCATCGT CTTGCGAGAA GATAATTGCC ATTTCTTAAG AGTGGACAAG
    GAGGATTTCA ACCGAATCCT GAGGGACGTT GAGGCGAATA CAGTCAGACT TAAAGAACAT
    GACCAAGATG TCTTGGTGCT GGAGAAGGTC CCAGCAGGGA ACAGGGCTTC TAATCAAGGA
    AACTCACAGC CTCAGCAAAA GTATACTGTG ATGTCAGGAA CACCTGAAAA AATTTTAGAG
    CATTTTCTAG AAACAATACG CCTTGAGCCA GCTTTAAATG AAGCAACAGA TTCTGTTTTA
    AACGACTTTG TTATGATGCA CTGTGTTTTT ATGCCAAATA TCCAGCTTTG TCCAGCCCTG
    GTGGCCCATT ACCATGCACA GCCCTCACAA GGTACAGAAC AGGAGAAAAT GGATTATGCC
    CTCAACAATA AGAGACGAGT CATCCGGCTG GTTTTACAGT GGGCCGCCAT GTATGGAGAC
    GTCCTACAAG AGGATGACGT AGCTATGGCT TTCCTGGAGG AGTTTTATGT GTCTGTGTCA
    GATGATGCCC GGATGATTGC TGCCCTCAAG GAGCAACTGC CCGAGCTGGA GAAGATCGTC
    AAGCAAATTT CAGAAGATGC AAAAGCTCCA CAAAAGAAGC ACAAAGTTCT TTTGCAACAG
    TTCAACACAG GCGATGAGAG AGCCCAGAAG CGCCAGCCTA TCCGTGGCTC TGATGAAGTT
    CTGTTTAAGG TCTATTGCAT GGACCACACC TACACAACCA TTCGGGTGCC GGTGGCCGCC
    TCAGTGAAGG AAGTCATCAG CGCAGTTGCC GACAAACTGG GCTCCGGAGA AGGCCTGATC
    ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG GTGGTGCTCA AACCTAATGA TGTTTCAGTA
    TTTACAACGC TCACCATTAA TGGACGCCTG TTTGCTTGCC CGCGAGAGCA ATTCGACTCA
    CTGACTCCCC TGCCAGAACA GGAAGGCCCA ACTGTTGGAA CAGTGGGAAC TTTTGAACTG
    ATGAGCTCCA AAGATTTAGC ATACCAGATG ACAATTTATG ATTGGGAACT CTTCAACTGT
    GTGCATGAGC TGGAGCTAAT CTATCACACA TTTGGAAGGC ATAATTTTAA AAAGACCACA
    GCAAACTTGG ATTTGTTCCT GAGAAGATTT AATGAAATTC AGTTTTGGGT TGTCACCGAG
    ATTTGCCTTT GTCCCCAGCC CAGCAAGCGT GTTCAGCTCT TAAAAAAATT TATTAAGATC
    GCAGCCCACT GTAAGGAGTA CAAAAATCTG AATTCCTTTT TTGCCATCGT CATGGGACTA
    AGTAATGTTG CCGTGAGCCG TTTGGCACTA ACGTGGGAGA AACTGCCAAG CAAGTTCAAG
    AAGTTCTATG CGGAGTTTGA AAGCTTAATG GATCCTTCGA GAAACCACAG GGCCTACCGG
    CTGACCATAG CTAAGCTGGA CCCCCCTCTC ATCCCCTTCA TGCCTTTGCT CATTAAAGAT
    ATGACATTTA CTCATGAGGG GAACAAGACA TTCATTGACA ATCTAGTAAA CTTTGAAAAA
    ATGCGCATGA TTGCAAATAC TGCCAGAACA GTGAGATACT GCAGGAGCCA ACCCTTCAAT
    CCTGATGCCG CTCAAGCTAA TAAGAACCAT CAGGATGTCC GGAGTTATGT ACGGCAATTA
    AATGTGATTG ACAACCAGAG AACTTTATCA CAGATGTCAC ACAGATTAGA GCCTCGTCGA
    CCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023477767.1
    CDS97..3102
    Translation

    Target ORF information:

    RefSeq Version XM_023621999.1
    Organism Equus caballus(horse)
    Definition Equus caballus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023621999.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTGCTTCT CTCTGCTGAT ACTCGTGGGC GTGTATCCTG TGCCTCAGGT GGGTTGGCAT 
    GGATGGAAGA TTGAGACTCA CCGTTCTAGA CCGCTGGAAC GATCCAGTGA AGATGTAGAT
    ATAATCTTCA CACGGCTGAA AGAAGTTAAA GCTTTTGAGA AATTTCACCC AAATCTCCTT
    CATCAGATTT GTTTGTGTGG TTATTATGAG AATCTGGAAA AAGGAATAAC ATTATTTCGC
    CAGGGTGATA TTGGAACAAA CTGGTATGCT GTCCTGGCAG GGTCTTTGGA TGTTAAAGTA
    TCTGAAACCA GCAGTCACCA GGATGCAGTG ACCATCTGTA CTCTGGGAAT TGGGACAGCC
    TTTGGAGAGT CCATTCTGGA CAACACACCC CGCCATGCGA CCATCGTTAC CAGGGAGAGC
    AGCGAACTGC TCCGCATCGA GCAGAAGGAC TTCAAGGCAC TATGGGAGAA ATATCGACAG
    TACATGGCAG GACTTCTGGC TCCTCCTTAT GGTGTTATGG AAACGGGCTC TAACAATGAC
    AGGATTCCTG ACAAGGAGAA TGTCCCTTCA GAGAAGATCC TCAGGGCTGG AAAAATTTTA
    CGAAACGCCA TTCTCTCTCG AGCACCTCAC ATGATTAGAG ATAGGAAATA CCACCTAAAG
    ACGTACAGAC AATGCTGTGT GGGAACTGAG CTGGTGGACT GGATGCTGCA GCAGACTCCG
    TGTGCCCACT CCCGGACTCA AGCTGTTGGG ATGTGGCAAG TGCTGGTGGA AGATGGTGTT
    CTCAACCATG TGGACCAGGA GCATCATTTC CAAGACAAAT ATTTATTTTA TCGATTTCTG
    GATGACGAGC ACGAGGATGC CCCTTTGCCT ACTGAGGAGG AGAAGAAGGA GTGTGATGAG
    GAGCTCCAGG ACACCATGCT GCTGCTGTCA CAGATGGGCC CCGATGCCCA CATGAGGATG
    ATCCTCCGCA AACCACCTGG CCAGAGGACT GTGGATGACC TAGAAATTAT CTACGAAGAG
    CTCCTTCACA TTAAAGCCTT ATCCCACCTT TCCACCACTG TGAAACGGGA GTTGGCAGGT
    GTTCTCATTT TTGAGTCGCA CGCCAAAGGA GGAACCGTGT TGTTCAACCA GGGGGAAGAA
    GGTACCTCCT GGTACATTAT TCTAAAAGGA TCGGTGAATG TAGTCATTTA TGGCAAGGGT
    GTGGTCTGCA CCCTGCATGA GGGAGATGAC TTTGGCAAGC TAGCACTAGT GAACGACGCC
    CCGAGAGCTG CCTCCATCGT CTTGCGAGAA GATAATTGCC ATTTCTTAAG AGTGGACAAG
    GAGGATTTCA ACCGAATCCT GAGGGACGTT GAGGCGAATA CAGTCAGACT TAAAGAACAT
    GACCAAGATG TCTTGGTGCT GGAGAAGGTC CCAGCAGGGA ACAGGGCTTC TAATCAAGGA
    AACTCACAGC CTCAGCAAAA GTATACTGTG ATGTCAGGAA CACCTGAAAA AATTTTAGAG
    CATTTTCTAG AAACAATACG CCTTGAGCCA GCTTTAAATG AAGCAACAGA TTCTGTTTTA
    AACGACTTTG TTATGATGCA CTGTGTTTTT ATGCCAAATA TCCAGCTTTG TCCAGCCCTG
    GTGGCCCATT ACCATGCACA GCCCTCACAA GGTACAGAAC AGGAGAAAAT GGATTATGCC
    CTCAACAATA AGAGACGAGT CATCCGGCTG GTTTTACAGT GGGCCGCCAT GTATGGAGAC
    GTCCTACAAG AGGATGACGT AGCTATGGCT TTCCTGGAGG AGTTTTATGT GTCTGTGTCA
    GATGATGCCC GGATGATTGC TGCCCTCAAG GAGCAACTGC CCGAGCTGGA GAAGATCGTC
    AAGCAAATTT CAGAAGATGC AAAAGCTCCA CAAAAGAAGC ACAAAGTTCT TTTGCAACAG
    TTCAACACAG GCGATGAGAG AGCCCAGAAG CGCCAGCCTA TCCGTGGCTC TGATGAAGTT
    CTGTTTAAGG TCTATTGCAT GGACCACACC TACACAACCA TTCGGGTGCC GGTGGCCGCC
    TCAGTGAAGG AAGTCATCAG CGCAGTTGCC GACAAACTGG GCTCCGGAGA AGGCCTGATC
    ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG GTGGTGCTCA AACCTAATGA TGTTTCAGTA
    TTTACAACGC TCACCATTAA TGGACGCCTG TTTGCTTGCC CGCGAGAGCA ATTCGACTCA
    CTGACTCCCC TGCCAGAACA GGAAGGCCCA ACTGTTGGAA CAGTGGGAAC TTTTGAACTG
    ATGAGCTCCA AAGATTTAGC ATACCAGATG ACAATTTATG ATTGGGAACT CTTCAACTGT
    GTGCATGAGC TGGAGCTAAT CTATCACACA TTTGGAAGGC ATAATTTTAA AAAGACCACA
    GCAAACTTGG ATTTGTTCCT GAGAAGATTT AATGAAATTC AGTTTTGGGT TGTCACCGAG
    ATTTGCCTTT GTCCCCAGCC CAGCAAGCGT GTTCAGCTCT TAAAAAAATT TATTAAGATC
    GCAGCCCACT GTAAGGAGTA CAAAAATCTG AATTCCTTTT TTGCCATCGT CATGGGACTA
    AGTAATGTTG CCGTGAGCCG TTTGGCACTA ACGTGGGAGA AACTGCCAAG CAAGTTCAAG
    AAGTTCTATG CGGAGTTTGA AAGCTTAATG GATCCTTCGA GAAACCACAG GGCCTACCGG
    CTGACCATAG CTAAGCTGGA CCCCCCTCTC ATCCCCTTCA TGCCTTTGCT CATTAAAGAT
    ATGACATTTA CTCATGAGGG GAACAAGACA TTCATTGACA ATCTAGTAAA CTTTGAAAAA
    ATGCGCATGA TTGCAAATAC TGCCAGAACA GTGAGATACT GCAGGAGCCA ACCCTTCAAT
    CCTGATGCCG CTCAAGCTAA TAAGAACCAT CAGGATGTCC GGAGTTATGT ACGGCAATTA
    AATGTGATTG ACAACCAGAG AACTTTATCA CAGATGTCAC ACAGATTAGA GCCTCGTCGA
    CCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.