NSMCE2 cDNA ORF clone, Felis catus(domestic cat)

Gene
Clones
gRNAs

Summary

Gene Symbol	NSMCE2
Entrez Gene ID	101086785
Full Name	NSE2/MMS21 homolog, SMC5-SMC6 complex SUMO ligase
Gene Type	protein-coding
Organism	Felis catus(domestic cat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004000117.4	XP_004000166.1	E3 SUMO-protein ligase NSE2 isoform X2
XM_019823121.1	XP_019678680.1	E3 SUMO-protein ligase NSE2 isoform X1

Pathways from BioSystems
Gene Ontology

The following NSMCE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NSMCE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFb23259	XM_004000117.4 Latest version!	Felis catus NSE2/MMS21 homolog, SMC5-SMC6 complex SUMO ligase (NSMCE2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFb57288	XM_019823121.1 Latest version!	Felis catus NSE2/MMS21 homolog, SMC5-SMC6 complex SUMO ligase (NSMCE2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NSMCE2 ( XM_004000117.4 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_004000166.1 Felis catus NSE2/MMS21 homolog, SMC5-SMC6 complex SUMO ligase (NSMCE2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OFb23259
Clone ID Related Accession (Same CDS sequence)	XM_004000117.4
Accession Version	XM_004000117.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	E3 SUMO-protein ligase NSE2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018740.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_004000117.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGAC GTTCGAGTTC AAGTTCAGGC TCAACTGGTT TTATATCCTT CAGTGGTATA 
GAATCTGCTC TCTCCACTTT GAAAAACTTC CAATCCTGTA TCAGCTCTGG AATGGACACA 
GCTTCTAGTG TTGCCTTGGA TCTTGTGGAA ACTCAGACTG AAGTGAGTAG TGAATACAGT 
ATGGACAAGG CAATGGTTGA ATTGGCCATG ATGGATCGGG AACTAAGCCA TTACGTAAAG 
GCTGTTCAGT CTGCGATAAA TCATGTAAAA GAAGAACGTC CAGAGAAGAT ACCAGATCTA 
AAATTATTAG TGGAGAAGAA ATTTTTGGCT TTACAGAATA AGAATTGTGA CACAGACTTT 
CAAAATAATG CAAAATTTGT ACAGTTTAAA CAACAGCTGA AAGAACTGAA GAAGCAATAT 
GGTCTTCAAG CCGACAGAGA GGTTGATGGA ACAGAAGGAG TTGATGAAGA TATGATTGTG 
ACCCAGAGCC AGACCAACTT CATCTGCCCC ATTACACAGG TGGAAATGAA GAAACCGGTG 
AAAAATAAAG TGTGTGGCCA CACCTATGAA GAGGAAGCCA TTGTTCGCAT GATTGAGTCC 
AAGCACAGGC GGAAGAAAAA GGCCTGTTGC CCCAAAATTG GCTGTAGCCA CACAGATGTG 
AGAATGTCAG ACCTCATCCA GGATGAAGCA CTTAGAAGGG CCATCGAGAG CCACAACAAG 
AAGAAAACTC GTCACTCCGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004000166.1
CDS	121..864
Translation	MPGRSSSSSGSTGFISFSGIESALSTLKNFQSCISSGMDTASSVALDLVETQTEVSSEYSMDKAMVELAMMDRELSHYVKAVQSAINHVKEERPEKIPDLKLLVEKKFLALQNKNCDTDFQNNAKFVQFKQQLKELKKQYGLQADREVDGTEGVDEDMIVTQSQTNFICPITQVEMKKPVKNKVCGHTYEEEAIVRMIESKHRRKKKACCPKIGCSHTDVRMSDLIQDEALRRAIESHNKKKTRHSE

Target ORF information:

RefSeq Version	XM_004000117.4
Organism	Felis catus(domestic cat)
Definition	Felis catus NSE2/MMS21 homolog, SMC5-SMC6 complex SUMO ligase (NSMCE2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004000117.4

ORF Insert Sequence:

ATGCCCGGAC GTTCGAGTTC AAGTTCAGGC TCAACTGGTT TTATATCCTT CAGTGGTATA 
GAATCTGCTC TCTCCACTTT GAAAAACTTC CAATCCTGTA TCAGCTCTGG AATGGACACA 
GCTTCTAGTG TTGCCTTGGA TCTTGTGGAA ACTCAGACTG AAGTGAGTAG TGAATACAGT 
ATGGACAAGG CAATGGTTGA ATTGGCCATG ATGGATCGGG AACTAAGCCA TTACGTAAAG 
GCTGTTCAGT CTGCGATAAA TCATGTAAAA GAAGAACGTC CAGAGAAGAT ACCAGATCTA 
AAATTATTAG TGGAGAAGAA ATTTTTGGCT TTACAGAATA AGAATTGTGA CACAGACTTT 
CAAAATAATG CAAAATTTGT ACAGTTTAAA CAACAGCTGA AAGAACTGAA GAAGCAATAT 
GGTCTTCAAG CCGACAGAGA GGTTGATGGA ACAGAAGGAG TTGATGAAGA TATGATTGTG 
ACCCAGAGCC AGACCAACTT CATCTGCCCC ATTACACAGG TGGAAATGAA GAAACCGGTG 
AAAAATAAAG TGTGTGGCCA CACCTATGAA GAGGAAGCCA TTGTTCGCAT GATTGAGTCC 
AAGCACAGGC GGAAGAAAAA GGCCTGTTGC CCCAAAATTG GCTGTAGCCA CACAGATGTG 
AGAATGTCAG ACCTCATCCA GGATGAAGCA CTTAGAAGGG CCATCGAGAG CCACAACAAG 
AAGAAAACTC GTCACTCCGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSMCE2 ( XM_019823121.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019678680.1 Felis catus NSE2/MMS21 homolog, SMC5-SMC6 complex SUMO ligase (NSMCE2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OFb57288
Clone ID Related Accession (Same CDS sequence)	XM_019823121.1
Accession Version	XM_019823121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	E3 SUMO-protein ligase NSE2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018740.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGAC GTTCGAGTTC AAGTTCAGGC TCAACTGGTT TTATATCCTT CAGTGGTATA 
GAATCTGCTC TCTCCACTTT GAAAAACTTC CAATCCTGTA TCAGCTCTGG AATGGACACA 
GCTTCTAGTG TTGCCTTGGA TCTTGTGGAA ACTCAGACTG AAGTGAGTAG TGAATACAGT 
ATGGACAAGG CAATGGTTGA ATTGGCCATG ATGGATCGGG AACTAAGCCA TTACGTAAAG 
GCTGTTCAGT CTGCGATAAA TCATGTAAAA GAAGAACGTC CAGAGAAGAT ACCAGATCTA 
AAATTATTAG TGGAGAAGAA ATTTTTGGCT TTACAGAATA AGAATTGTGA CACAGACTTT 
CAAAATAATG CAAAATTTGT ACAGTTTAAA CAACAGCTGA AAGAACTGAA GAAGCAATCA 
GCGAAACCTA AAGAAGATAG AAGACACAAT GAGGTCGACA AGATTAAGGA TAAGGATTCA 
AGTAGAGCAG GCAATGGACG AGATGGTCTT CAAGCCGACA GAGAGGTTGA TGGAACAGAA 
GGAGTTGATG AAGATATGAT TGTGACCCAG AGCCAGACCA ACTTCATCTG CCCCATTACA 
CAGGTGGAAA TGAAGAAACC GGTGAAAAAT AAAGTGTGTG GCCACACCTA TGAAGAGGAA 
GCCATTGTTC GCATGATTGA GTCCAAGCAC AGGCGGAAGA AAAAGGCCTG TTGCCCCAAA 
ATTGGCTGTA GCCACACAGA TGTGAGAATG TCAGACCTCA TCCAGGATGA AGCACTTAGA 
AGGGCCATCG AGAGCCACAA CAAGAAGAAA ACTCGTCACT CCGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019678680.1
CDS	121..948
Translation	MPGRSSSSSGSTGFISFSGIESALSTLKNFQSCISSGMDTASSVALDLVETQTEVSSEYSMDKAMVELAMMDRELSHYVKAVQSAINHVKEERPEKIPDLKLLVEKKFLALQNKNCDTDFQNNAKFVQFKQQLKELKKQSAKPKEDRRHNEVDKIKDKDSSRAGNGRDGLQADREVDGTEGVDEDMIVTQSQTNFICPITQVEMKKPVKNKVCGHTYEEEAIVRMIESKHRRKKKACCPKIGCSHTDVRMSDLIQDEALRRAIESHNKKKTRHSE

Target ORF information:

RefSeq Version	XM_019823121.1
Organism	Felis catus(domestic cat)
Definition	Felis catus NSE2/MMS21 homolog, SMC5-SMC6 complex SUMO ligase (NSMCE2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019823121.1

ORF Insert Sequence:

ATGCCCGGAC GTTCGAGTTC AAGTTCAGGC TCAACTGGTT TTATATCCTT CAGTGGTATA 
GAATCTGCTC TCTCCACTTT GAAAAACTTC CAATCCTGTA TCAGCTCTGG AATGGACACA 
GCTTCTAGTG TTGCCTTGGA TCTTGTGGAA ACTCAGACTG AAGTGAGTAG TGAATACAGT 
ATGGACAAGG CAATGGTTGA ATTGGCCATG ATGGATCGGG AACTAAGCCA TTACGTAAAG 
GCTGTTCAGT CTGCGATAAA TCATGTAAAA GAAGAACGTC CAGAGAAGAT ACCAGATCTA 
AAATTATTAG TGGAGAAGAA ATTTTTGGCT TTACAGAATA AGAATTGTGA CACAGACTTT 
CAAAATAATG CAAAATTTGT ACAGTTTAAA CAACAGCTGA AAGAACTGAA GAAGCAATCA 
GCGAAACCTA AAGAAGATAG AAGACACAAT GAGGTCGACA AGATTAAGGA TAAGGATTCA 
AGTAGAGCAG GCAATGGACG AGATGGTCTT CAAGCCGACA GAGAGGTTGA TGGAACAGAA 
GGAGTTGATG AAGATATGAT TGTGACCCAG AGCCAGACCA ACTTCATCTG CCCCATTACA 
CAGGTGGAAA TGAAGAAACC GGTGAAAAAT AAAGTGTGTG GCCACACCTA TGAAGAGGAA 
GCCATTGTTC GCATGATTGA GTCCAAGCAC AGGCGGAAGA AAAAGGCCTG TTGCCCCAAA 
ATTGGCTGTA GCCACACAGA TGTGAGAATG TCAGACCTCA TCCAGGATGA AGCACTTAGA 
AGGGCCATCG AGAGCCACAA CAAGAAGAAA ACTCGTCACT CCGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.