LOC611458 cDNA ORF clone, Canis lupus familiaris(Dog)

The following LOC611458 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC611458 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe282889 XM_022411257.1
Latest version!
Canis lupus familiaris pregnancy zone protein-like (LOC611458), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe27344 XM_005637173.3
Latest version!
Canis lupus familiaris pregnancy zone protein-like (LOC611458), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe27345 XM_005637174.3
Latest version!
Canis lupus familiaris pregnancy zone protein-like (LOC611458), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OCe27345 XM_005637174.2 Canis lupus familiaris alpha-2-macroglobulin-like (LOC611458), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe27344 XM_005637173.2 Canis lupus familiaris alpha-2-macroglobulin-like (LOC611458), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe282889
    Clone ID Related Accession (Same CDS sequence) XM_022411257.1
    Accession Version XM_022411257.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3609bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pregnancy zone protein-like isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTCATTTT TGGCTTGGGA AAGGGCAGAT GATAAAGGCT GTTTCACACA ACTCGTGAAG 
    ACCAAAGTAT TTCAGCTGAG ACAAAGGGGG TATGACATGA CAATACAAGT GGAAGCCAAA
    ATCAAAGAGG AGGGAACAGA GGTGGAACTA ACTGGGCATG GATCATGTGA AATCACAAAT
    ATCCTGAGCA AGCTGAAATT TACGAAAGCG GATACATATT ATAGACCTGG GCTTCCTTTT
    TATGGGCAGG TTCTTCTAGT TGACGAGAAG GATCAGCCAA TGGCCCATAA AACTGTAGTC
    GTCAGTGTGG ACGTAACCAC ATACCAATCC AGCCTTACCA CGAATGAACA TGGTTTGGTG
    AACATTTACA TTGATACCAC CAACTTCACA TCTTCTCTCT TTACAGTTAT GGTGACTTAC
    AAACAGAATG TCCACTGTTT TGATAATTGG TGGCTTGAAG AACTTCATAA CCAAGCACAG
    CACACTGTAA AGCATATTTT CTCCCCGAGC AAGAGTTATG TTCACCTTGA ACTTGTTGCT
    GGTACCATAA CCTGTGGACA AACCCAGGAG ATCCGGGCGC ACTACATCCT GAATGGACAG
    ATTCTGAAGG ATGAAAAAGA ATTAACCTTC TATTATTTGA TCAAAGCAAG AGGAATGATC
    TCCCGATCAG GAATCCATGT GCTGTCCATT GAACAAGGAG ACTTGAAAGG GGAATTTTCC
    TTCTCCTTTC GAGTGGAATC AGACATTGCT CCTGCAGCCC AGCTGCTCAT TTATGCTATT
    TTGCCCAATG GAGAAATTGT TGCCGATACT GAGAAACTCG AGATTGAAAA CTGTTTTGCC
    AATAAGGTAA ATTTGAGCTT CTCCTCAAAC CAGAGCCTGC CGGCCTCACA CACCAACCTA
    GAGGTCAGGG CCTTTCCTAA CTCCCTCTGT GCCCTCCGAG CAGTAGACCA GAGTGTTCTG
    CTGATGAAGC CTGAAACTGA GCTCTCCCCT CAGTCGGTCT ATAATTTGCT ACCAGTAAAG
    GGTATCTTTA GTGTTGGTTA TGGAGATCTC AGAAGAGAAG ATAGTGAGCA ATGTATCCAC
    GCAGAAGACT TTATTCACGA GGGGATCATT TACACGCCAA AGCAGATCCT GGATAATGAT
    GACATTTACA GTATTTTTGA GTCCGCAGGA TTATATATTT TTACCAACTC AAAAATCCAC
    AAGCCGAATT TTTGTCAGCT GCCACAGCCA TATCCGGGAG TGCCTATGGC CCATGCAGCA
    GTTAACGGTG AGATTTATTT GCCAGTCCTG CCTCTAGCAC GTGGTAGTGC AGGAGCAGTT
    TATCCAATGG TCATGAGACA CAGTTCTCCA GCCGTGGAAG TGAAAGAGAC AGTGCGGAAG
    TACTTCCCTG AGACCTGGAT CTGGGACTTG GTACCAGTCG ATTTATCAGG TAGAAGTGAG
    CTGGCAGTGA AGGTCCCTGA CACCATCACA GAGTGGAAGG CCAGTGCCCT CTGCTTGTCA
    GAAATGACAG GGCTAGGCCT CTCCCCCACC ATCTCTCTTC AAGTGTTCCA GCCTTTTTTC
    CTGGAACTCA CTCTTCCCTA CTCTGTGGTG CGAGGAGAAG CCTTCACACT CAAAGCAACC
    GTGTTCAACT ATTTGTCCCA CTGCATTCGG GTCAGTGTGC AGCTGGAAGC CTCTCCTGAC
    AAGTCGGCTG ACCTAGAGAG AAAGAACGAA GAAACTCCCT GTATCTGTGG AAATCACCAG
    AAAACTATGT TCTGGGCTGT GACCCCAAAG TCACTGGGAA AGATGAATTT CACAGCTACT
    GTGGAAGCCT TGCAGTCTCA GGAATTGTGT GGCAATGAGG TGCCAAGAGT CCCAGAACTT
    GGACAGAAGG ATACTGTAGT CAAGCCCTTA TTAGTTGAGC CTGAAGGAAT AGAAAAGGAG
    GAAACTTTCA ACACACTCCT CTGTGCAGCA GAAACTGATG TACCTGAAAA GTTGTCACTA
    AAAGTTCCTT CAGATGTGGT TGAAGGATCT GCCAGGGCGA CATATTCGGT TTTGGGTGAC
    ATTCTAGGCT CTGCAATGCA AAATCTTCAG AATCTTCTCC GGATGCCATA TGGTTGTGGA
    GAGCAAAATA TGGTCCTATT TGTCCCTAAC ATCTATGTTC TGAACTACCT GAATGAGACA
    CGACAACTGA CAGAGACTAT CAAGTCCAAA GCCATCAACT ACCTCATCAG TGGGTACCAA
    AGGCAGTTGA ATTATAAGCA CAGTGATGGT TCATACAGCA CCTTTGGGGA TCATGATGGA
    AGAAGTCAGG GAAACACTTG GCTCACTGCA TTTGTGCTCA AGTCTTTTGC TCAAGCCCGG
    TCACACATTT TTGTGGAGAT GTCACACATC ACGAGTTCCC TCACCTGGCT CTCACAAAGA
    CAGAAGGAAA ATGGTTGTTT CCAACGCTCT GGATCACTAC TAAACAATGC TGTTAAGGGT
    GGGGTAGATG ATGAATTGAC GCTCTCTGCC TACATCACCA TTGCTCTGCT GGAGATGCCA
    CTGCCTGTCA CTCACCCTCT TGTCCGCAAT GCTCTATTCT GCCTGGAAAG TGCCTGGGGA
    TCCATCTCAG AGGCCCAAGG AAGTCTTGTC TATACGAAGG CATTATTGGC TTATGCCTTT
    GCCCTAGCAG GAAACCAGGT CAAGCGAAAT GAGCTTCTTA AATCACTAGA CAAAGATGCT
    GTGAAAGAAG AGGACTCAAT CCACTGGCAG CGTCCTGGGA AAGTTGAAGA AGTTACGACA
    TTCTATCAAC CCCGGGCTCC TTCAGTTGAA GTGGAGATGA CCTCTTACCT GCTCCTTGCC
    TATCTTACTG CTTCGCCTGC CCCATCTTCA GAAGACCTGT CTGTGGCTTC ACGTATTGTG
    AAATGGATCA CCAAGCAACA AAACGCCAAT GGGGGCTTCT CTTCCACTCA GGATACAGTG
    GTAGCTCTCC AAGCCCTGTC CAAATATGGA GCAGCAACTT TCAGTAAAAG GGAGAAAACA
    ACTGTAGTCA CTGTCAAGTC TTCAGAGACA TTCTCCCAAA AATTCGAAGT ACACGATGCC
    AATCGCCTGT TGCTACAAGA AGTCAGGTTG CCAGAGATTC CAGGGGAGTA CAGCACAACA
    GTGTCAGGAT CAGGATGTGT GTACCTCCAG ACATCCCTGA GATACAACAT CCTGCCCAAG
    AAAGAAGGCA AAGTTCCCTT CAATCTGAAG GTTGATACTT TCCCCAAGAA TTGTGATGGA
    GTAGACACTC ACAGGAAATT CCAGATTCAC ATCAACATTA GTTATACTGG GGAACGATCA
    AGCTCCAACA TGGTCATTGT TGACGTGAAG ATGGTATCAG GCTTCATACC CGTGAAATCA
    TCAGTGAGAA AGCTCCAAGA AAAGCCTCAG ATTCAAAGGA CTGAAGTGAG CACCAACCAT
    GTTCTGATTT ACTTTGAAGA GCTAACCAAT CAAACTCTGA GTTTCTCCTT CTCAGTGGAA
    CAAGACATCC AAGTTGACAA TTTAAAACCA GCTACTGTAA AAGCCTATGA CTATTATGAG
    ACAGAGGAAT TTACTATTGA AGAGTACAGG GATCCCTGCA GTGCTGAATC TGGACACAGA
    ACTGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266965.1
    CDS85..3693
    Translation

    Target ORF information:

    RefSeq Version XM_022411257.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pregnancy zone protein-like (LOC611458), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022411257.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTCATTTT TGGCTTGGGA AAGGGCAGAT GATAAAGGCT GTTTCACACA ACTCGTGAAG 
    ACCAAAGTAT TTCAGCTGAG ACAAAGGGGG TATGACATGA CAATACAAGT GGAAGCCAAA
    ATCAAAGAGG AGGGAACAGA GGTGGAACTA ACTGGGCATG GATCATGTGA AATCACAAAT
    ATCCTGAGCA AGCTGAAATT TACGAAAGCG GATACATATT ATAGACCTGG GCTTCCTTTT
    TATGGGCAGG TTCTTCTAGT TGACGAGAAG GATCAGCCAA TGGCCCATAA AACTGTAGTC
    GTCAGTGTGG ACGTAACCAC ATACCAATCC AGCCTTACCA CGAATGAACA TGGTTTGGTG
    AACATTTACA TTGATACCAC CAACTTCACA TCTTCTCTCT TTACAGTTAT GGTGACTTAC
    AAACAGAATG TCCACTGTTT TGATAATTGG TGGCTTGAAG AACTTCATAA CCAAGCACAG
    CACACTGTAA AGCATATTTT CTCCCCGAGC AAGAGTTATG TTCACCTTGA ACTTGTTGCT
    GGTACCATAA CCTGTGGACA AACCCAGGAG ATCCGGGCGC ACTACATCCT GAATGGACAG
    ATTCTGAAGG ATGAAAAAGA ATTAACCTTC TATTATTTGA TCAAAGCAAG AGGAATGATC
    TCCCGATCAG GAATCCATGT GCTGTCCATT GAACAAGGAG ACTTGAAAGG GGAATTTTCC
    TTCTCCTTTC GAGTGGAATC AGACATTGCT CCTGCAGCCC AGCTGCTCAT TTATGCTATT
    TTGCCCAATG GAGAAATTGT TGCCGATACT GAGAAACTCG AGATTGAAAA CTGTTTTGCC
    AATAAGGTAA ATTTGAGCTT CTCCTCAAAC CAGAGCCTGC CGGCCTCACA CACCAACCTA
    GAGGTCAGGG CCTTTCCTAA CTCCCTCTGT GCCCTCCGAG CAGTAGACCA GAGTGTTCTG
    CTGATGAAGC CTGAAACTGA GCTCTCCCCT CAGTCGGTCT ATAATTTGCT ACCAGTAAAG
    GGTATCTTTA GTGTTGGTTA TGGAGATCTC AGAAGAGAAG ATAGTGAGCA ATGTATCCAC
    GCAGAAGACT TTATTCACGA GGGGATCATT TACACGCCAA AGCAGATCCT GGATAATGAT
    GACATTTACA GTATTTTTGA GTCCGCAGGA TTATATATTT TTACCAACTC AAAAATCCAC
    AAGCCGAATT TTTGTCAGCT GCCACAGCCA TATCCGGGAG TGCCTATGGC CCATGCAGCA
    GTTAACGGTG AGATTTATTT GCCAGTCCTG CCTCTAGCAC GTGGTAGTGC AGGAGCAGTT
    TATCCAATGG TCATGAGACA CAGTTCTCCA GCCGTGGAAG TGAAAGAGAC AGTGCGGAAG
    TACTTCCCTG AGACCTGGAT CTGGGACTTG GTACCAGTCG ATTTATCAGG TAGAAGTGAG
    CTGGCAGTGA AGGTCCCTGA CACCATCACA GAGTGGAAGG CCAGTGCCCT CTGCTTGTCA
    GAAATGACAG GGCTAGGCCT CTCCCCCACC ATCTCTCTTC AAGTGTTCCA GCCTTTTTTC
    CTGGAACTCA CTCTTCCCTA CTCTGTGGTG CGAGGAGAAG CCTTCACACT CAAAGCAACC
    GTGTTCAACT ATTTGTCCCA CTGCATTCGG GTCAGTGTGC AGCTGGAAGC CTCTCCTGAC
    AAGTCGGCTG ACCTAGAGAG AAAGAACGAA GAAACTCCCT GTATCTGTGG AAATCACCAG
    AAAACTATGT TCTGGGCTGT GACCCCAAAG TCACTGGGAA AGATGAATTT CACAGCTACT
    GTGGAAGCCT TGCAGTCTCA GGAATTGTGT GGCAATGAGG TGCCAAGAGT CCCAGAACTT
    GGACAGAAGG ATACTGTAGT CAAGCCCTTA TTAGTTGAGC CTGAAGGAAT AGAAAAGGAG
    GAAACTTTCA ACACACTCCT CTGTGCAGCA GAAACTGATG TACCTGAAAA GTTGTCACTA
    AAAGTTCCTT CAGATGTGGT TGAAGGATCT GCCAGGGCGA CATATTCGGT TTTGGGTGAC
    ATTCTAGGCT CTGCAATGCA AAATCTTCAG AATCTTCTCC GGATGCCATA TGGTTGTGGA
    GAGCAAAATA TGGTCCTATT TGTCCCTAAC ATCTATGTTC TGAACTACCT GAATGAGACA
    CGACAACTGA CAGAGACTAT CAAGTCCAAA GCCATCAACT ACCTCATCAG TGGGTACCAA
    AGGCAGTTGA ATTATAAGCA CAGTGATGGT TCATACAGCA CCTTTGGGGA TCATGATGGA
    AGAAGTCAGG GAAACACTTG GCTCACTGCA TTTGTGCTCA AGTCTTTTGC TCAAGCCCGG
    TCACACATTT TTGTGGAGAT GTCACACATC ACGAGTTCCC TCACCTGGCT CTCACAAAGA
    CAGAAGGAAA ATGGTTGTTT CCAACGCTCT GGATCACTAC TAAACAATGC TGTTAAGGGT
    GGGGTAGATG ATGAATTGAC GCTCTCTGCC TACATCACCA TTGCTCTGCT GGAGATGCCA
    CTGCCTGTCA CTCACCCTCT TGTCCGCAAT GCTCTATTCT GCCTGGAAAG TGCCTGGGGA
    TCCATCTCAG AGGCCCAAGG AAGTCTTGTC TATACGAAGG CATTATTGGC TTATGCCTTT
    GCCCTAGCAG GAAACCAGGT CAAGCGAAAT GAGCTTCTTA AATCACTAGA CAAAGATGCT
    GTGAAAGAAG AGGACTCAAT CCACTGGCAG CGTCCTGGGA AAGTTGAAGA AGTTACGACA
    TTCTATCAAC CCCGGGCTCC TTCAGTTGAA GTGGAGATGA CCTCTTACCT GCTCCTTGCC
    TATCTTACTG CTTCGCCTGC CCCATCTTCA GAAGACCTGT CTGTGGCTTC ACGTATTGTG
    AAATGGATCA CCAAGCAACA AAACGCCAAT GGGGGCTTCT CTTCCACTCA GGATACAGTG
    GTAGCTCTCC AAGCCCTGTC CAAATATGGA GCAGCAACTT TCAGTAAAAG GGAGAAAACA
    ACTGTAGTCA CTGTCAAGTC TTCAGAGACA TTCTCCCAAA AATTCGAAGT ACACGATGCC
    AATCGCCTGT TGCTACAAGA AGTCAGGTTG CCAGAGATTC CAGGGGAGTA CAGCACAACA
    GTGTCAGGAT CAGGATGTGT GTACCTCCAG ACATCCCTGA GATACAACAT CCTGCCCAAG
    AAAGAAGGCA AAGTTCCCTT CAATCTGAAG GTTGATACTT TCCCCAAGAA TTGTGATGGA
    GTAGACACTC ACAGGAAATT CCAGATTCAC ATCAACATTA GTTATACTGG GGAACGATCA
    AGCTCCAACA TGGTCATTGT TGACGTGAAG ATGGTATCAG GCTTCATACC CGTGAAATCA
    TCAGTGAGAA AGCTCCAAGA AAAGCCTCAG ATTCAAAGGA CTGAAGTGAG CACCAACCAT
    GTTCTGATTT ACTTTGAAGA GCTAACCAAT CAAACTCTGA GTTTCTCCTT CTCAGTGGAA
    CAAGACATCC AAGTTGACAA TTTAAAACCA GCTACTGTAA AAGCCTATGA CTATTATGAG
    ACAGAGGAAT TTACTATTGA AGAGTACAGG GATCCCTGCA GTGCTGAATC TGGACACAGA
    ACTGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27344
    Clone ID Related Accession (Same CDS sequence) XM_005637173.3 , XM_005637173.2
    Accession Version XM_005637173.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4455bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pregnancy zone protein-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005637173.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGGAGAA ATCCACTGCC CATCCCAGTC TTGATTCTGT TTTTCCTGCT CCTTCCCAGA 
    GATGCTTCCA CTACTTCAAA ACCGCAGTAC CTAGTGCTGG TCCCATCACA GCTGTACGCT
    AAGGTTCCTG AGAAGGCCTG TGTTATGCTT AATGACCTGA ATGAGACGGT GACAGTAAAC
    TTAATTCTGG AGTATGCAAT GCAAACCAGA ACCCTCCTCT CAGACGTGGT GACAAAGAAT
    TCCTTCTACT GCAGGCCCTT CACGATTCCA GAGTTGCCGT CGTCCCCAGG GCTCATTACC
    GTGGAGGTGA AGGGGCCAAC CCAACTGTTC ATAAAGAAGA AGAAAGTACG CATCACAAAA
    GTAAACAGCC TGATCTTTGT CCAAACAGAC AAACCCATCT ACAAGCCAGG ACAAACAGTG
    AGATTCCGTG TTGTCTCTGT GGATGTCAGT TTTCATCCTT TGAATGAAAC CTTGCCGGTG
    GTTTATATTG AGAATCCCAA AAGGAACCGA ATTTTCCAAT GGCAAAATCT CAGATTGCAA
    GGAGGACTCA GCCAGCTCTC CTTCCCACTC TCAGTGGAGC CCACCCTGGG TGCTTATAAG
    GTCAAGCTGG AGAAGGAGTC AGGGGAAAAA ATAGAGCACC CCTTTGAAGT GAAGGAATAC
    GTTTTGCCCA AATTTGAGGT CCAAGTGAAA ATGCCCAAGA CGATTAGTTT TCTGGATGAA
    GAATTTGTAG TATCCGCCTG TGGCCTATAC ACGTATGGAA AGCCTGTCTC TGGTGTGGTG
    ACAATTAACA TATGCAGAAA ATACTCACAA TATCATTTTG CCTGCCATGG AATGCATTCG
    CAAGGTGTCT GTGAAGAATT CAGTCAACAG GCAGATGATA AAGGCTGTTT CACACAACTC
    GTGAAGACCA AAGTATTTCA GCTGAGACAA AGGGGGTATG ACATGACAAT ACAAGTGGAA
    GCCAAAATCA AAGAGGAGGG AACAGAGGTG GAACTAACTG GGCATGGATC ATGTGAAATC
    ACAAATATCC TGAGCAAGCT GAAATTTACG AAAGCGGATA CATATTATAG ACCTGGGCTT
    CCTTTTTATG GGCAGGTTCT TCTAGTTGAC GAGAAGGATC AGCCAATGGC CCATAAAACT
    GTAGTCGTCA GTGTGGACGT AACCACATAC CAATCCAGCC TTACCACGAA TGAACATGGT
    TTGGTGAACA TTTACATTGA TACCACCAAC TTCACATCTT CTCTCTTTAC AGTTATGGTG
    ACTTACAAAC AGAATGTCCA CTGTTTTGAT AATTGGTGGC TTGAAGAACT TCATAACCAA
    GCACAGCACA CTGTAAAGCA TATTTTCTCC CCGAGCAAGA GTTATGTTCA CCTTGAACTT
    GTTGCTGGTA CCATAACCTG TGGACAAACC CAGGAGATCC GGGCGCACTA CATCCTGAAT
    GGACAGATTC TGAAGGATGA AAAAGAATTA ACCTTCTATT ATTTGATCAA AGCAAGAGGA
    ATGATCTCCC GATCAGGAAT CCATGTGCTG TCCATTGAAC AAGGAGACTT GAAAGGGGAA
    TTTTCCTTCT CCTTTCGAGT GGAATCAGAC ATTGCTCCTG CAGCCCAGCT GCTCATTTAT
    GCTATTTTGC CCAATGGAGA AATTGTTGCC GATACTGAGA AACTCGAGAT TGAAAACTGT
    TTTGCCAATA AGGTAAATTT GAGCTTCTCC TCAAACCAGA GCCTGCCGGC CTCACACACC
    AACCTAGAGG TCAGGGCCTT TCCTAACTCC CTCTGTGCCC TCCGAGCAGT AGACCAGAGT
    GTTCTGCTGA TGAAGCCTGA AACTGAGCTC TCCCCTCAGT CGGTCTATAA TTTGCTACCA
    GTAAAGGGTA TCTTTAGTGT TGGTTATGGA GATCTCAGAA GAGAAGATAG TGAGCAATGT
    ATCCACGCAG AAGACTTTAT TCACGAGGGG ATCATTTACA CGCCAAAGCA GATCCTGGAT
    AATGATGACA TTTACAGTAT TTTTGAGTCC GCAGGATTAT ATATTTTTAC CAACTCAAAA
    ATCCACAAGC CGAATTTTTG TCAGCTGCCA CAGCCATATC CGGGAGTGCC TATGGCCCAT
    GCAGCAGTTA ACGGTGAGAT TTATTTGCCA GTCCTGCCTC TAGCACGTGG TAGTGCAGGA
    GCAGTTTATC CAATGGTCAT GAGACACAGT TCTCCAGCCG TGGAAGTGAA AGAGACAGTG
    CGGAAGTACT TCCCTGAGAC CTGGATCTGG GACTTGGTAC CAGTCGATTT ATCAGGTAGA
    AGTGAGCTGG CAGTGAAGGT CCCTGACACC ATCACAGAGT GGAAGGCCAG TGCCCTCTGC
    TTGTCAGAAA TGACAGGGCT AGGCCTCTCC CCCACCATCT CTCTTCAAGT GTTCCAGCCT
    TTTTTCCTGG AACTCACTCT TCCCTACTCT GTGGTGCGAG GAGAAGCCTT CACACTCAAA
    GCAACCGTGT TCAACTATTT GTCCCACTGC ATTCGGGTCA GTGTGCAGCT GGAAGCCTCT
    CCTGACAAGT CGGCTGACCT AGAGAGAAAG AACGAAGAAA CTCCCTGTAT CTGTGGAAAT
    CACCAGAAAA CTATGTTCTG GGCTGTGACC CCAAAGTCAC TGGGAAAGAT GAATTTCACA
    GCTACTGTGG AAGCCTTGCA GTCTCAGGAA TTGTGTGGCA ATGAGGTGCC AAGAGTCCCA
    GAACTTGGAC AGAAGGATAC TGTAGTCAAG CCCTTATTAG TTGAGCCTGA AGGAATAGAA
    AAGGAGGAAA CTTTCAACAC ACTCCTCTGT GCAGCAGAAA CTGATGTACC TGAAAAGTTG
    TCACTAAAAG TTCCTTCAGA TGTGGTTGAA GGATCTGCCA GGGCGACATA TTCGGTTTTG
    GGTGACATTC TAGGCTCTGC AATGCAAAAT CTTCAGAATC TTCTCCGGAT GCCATATGGT
    TGTGGAGAGC AAAATATGGT CCTATTTGTC CCTAACATCT ATGTTCTGAA CTACCTGAAT
    GAGACACGAC AACTGACAGA GACTATCAAG TCCAAAGCCA TCAACTACCT CATCAGTGGG
    TACCAAAGGC AGTTGAATTA TAAGCACAGT GATGGTTCAT ACAGCACCTT TGGGGATCAT
    GATGGAAGAA GTCAGGGAAA CACTTGGCTC ACTGCATTTG TGCTCAAGTC TTTTGCTCAA
    GCCCGGTCAC ACATTTTTGT GGAGATGTCA CACATCACGA GTTCCCTCAC CTGGCTCTCA
    CAAAGACAGA AGGAAAATGG TTGTTTCCAA CGCTCTGGAT CACTACTAAA CAATGCTGTT
    AAGGGTGGGG TAGATGATGA ATTGACGCTC TCTGCCTACA TCACCATTGC TCTGCTGGAG
    ATGCCACTGC CTGTCACTCA CCCTCTTGTC CGCAATGCTC TATTCTGCCT GGAAAGTGCC
    TGGGGATCCA TCTCAGAGGC CCAAGGAAGT CTTGTCTATA CGAAGGCATT ATTGGCTTAT
    GCCTTTGCCC TAGCAGGAAA CCAGGTCAAG CGAAATGAGC TTCTTAAATC ACTAGACAAA
    GATGCTGTGA AAGAAGAGGA CTCAATCCAC TGGCAGCGTC CTGGGAAAGT TGAAGAAGTT
    ACGACATTCT ATCAACCCCG GGCTCCTTCA GTTGAAGTGG AGATGACCTC TTACCTGCTC
    CTTGCCTATC TTACTGCTTC GCCTGCCCCA TCTTCAGAAG ACCTGTCTGT GGCTTCACGT
    ATTGTGAAAT GGATCACCAA GCAACAAAAC GCCAATGGGG GCTTCTCTTC CACTCAGGAT
    ACAGTGGTAG CTCTCCAAGC CCTGTCCAAA TATGGAGCAG CAACTTTCAG TAAAAGGGAG
    AAAACAACTG TAGTCACTGT CAAGTCTTCA GAGACATTCT CCCAAAAATT CGAAGTACAC
    GATGCCAATC GCCTGTTGCT ACAAGAAGTC AGGTTGCCAG AGATTCCAGG GGAGTACAGC
    ACAACAGTGT CAGGATCAGG ATGTGTGTAC CTCCAGACAT CCCTGAGATA CAACATCCTG
    CCCAAGAAAG AAGGCAAAGT TCCCTTCAAT CTGAAGGTTG ATACTTTCCC CAAGAATTGT
    GATGGAGTAG ACACTCACAG GAAATTCCAG ATTCACATCA ACATTAGTTA TACTGGGGAA
    CGATCAAGCT CCAACATGGT CATTGTTGAC GTGAAGATGG TATCAGGCTT CATACCCGTG
    AAATCATCAG TGAGAAAGCT CCAAGAAAAG CCTCAGATTC AAAGGACTGA AGTGAGCACC
    AACCATGTTC TGATTTACTT TGAAGAGCTA ACCAATCAAA CTCTGAGTTT CTCCTTCTCA
    GTGGAACAAG ACATCCAAGT TGACAATTTA AAACCAGCTA CTGTAAAAGC CTATGACTAT
    TATGAGACAG AGGAATTTAC TATTGAAGAG TACAGGGATC CCTGCAGTGC TGAATCTGGA
    CACAGAACTG TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005637230.1
    CDS82..4536
    Translation

    Target ORF information:

    RefSeq Version XM_005637173.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pregnancy zone protein-like (LOC611458), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005637173.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGGAGAA ATCCACTGCC CATCCCAGTC TTGATTCTGT TTTTCCTGCT CCTTCCCAGA 
    GATGCTTCCA CTACTTCAAA ACCGCAGTAC CTAGTGCTGG TCCCATCACA GCTGTACGCT
    AAGGTTCCTG AGAAGGCCTG TGTTATGCTT AATGACCTGA ATGAGACGGT GACAGTAAAC
    TTAATTCTGG AGTATGCAAT GCAAACCAGA ACCCTCCTCT CAGACGTGGT GACAAAGAAT
    TCCTTCTACT GCAGGCCCTT CACGATTCCA GAGTTGCCGT CGTCCCCAGG GCTCATTACC
    GTGGAGGTGA AGGGGCCAAC CCAACTGTTC ATAAAGAAGA AGAAAGTACG CATCACAAAA
    GTAAACAGCC TGATCTTTGT CCAAACAGAC AAACCCATCT ACAAGCCAGG ACAAACAGTG
    AGATTCCGTG TTGTCTCTGT GGATGTCAGT TTTCATCCTT TGAATGAAAC CTTGCCGGTG
    GTTTATATTG AGAATCCCAA AAGGAACCGA ATTTTCCAAT GGCAAAATCT CAGATTGCAA
    GGAGGACTCA GCCAGCTCTC CTTCCCACTC TCAGTGGAGC CCACCCTGGG TGCTTATAAG
    GTCAAGCTGG AGAAGGAGTC AGGGGAAAAA ATAGAGCACC CCTTTGAAGT GAAGGAATAC
    GTTTTGCCCA AATTTGAGGT CCAAGTGAAA ATGCCCAAGA CGATTAGTTT TCTGGATGAA
    GAATTTGTAG TATCCGCCTG TGGCCTATAC ACGTATGGAA AGCCTGTCTC TGGTGTGGTG
    ACAATTAACA TATGCAGAAA ATACTCACAA TATCATTTTG CCTGCCATGG AATGCATTCG
    CAAGGTGTCT GTGAAGAATT CAGTCAACAG GCAGATGATA AAGGCTGTTT CACACAACTC
    GTGAAGACCA AAGTATTTCA GCTGAGACAA AGGGGGTATG ACATGACAAT ACAAGTGGAA
    GCCAAAATCA AAGAGGAGGG AACAGAGGTG GAACTAACTG GGCATGGATC ATGTGAAATC
    ACAAATATCC TGAGCAAGCT GAAATTTACG AAAGCGGATA CATATTATAG ACCTGGGCTT
    CCTTTTTATG GGCAGGTTCT TCTAGTTGAC GAGAAGGATC AGCCAATGGC CCATAAAACT
    GTAGTCGTCA GTGTGGACGT AACCACATAC CAATCCAGCC TTACCACGAA TGAACATGGT
    TTGGTGAACA TTTACATTGA TACCACCAAC TTCACATCTT CTCTCTTTAC AGTTATGGTG
    ACTTACAAAC AGAATGTCCA CTGTTTTGAT AATTGGTGGC TTGAAGAACT TCATAACCAA
    GCACAGCACA CTGTAAAGCA TATTTTCTCC CCGAGCAAGA GTTATGTTCA CCTTGAACTT
    GTTGCTGGTA CCATAACCTG TGGACAAACC CAGGAGATCC GGGCGCACTA CATCCTGAAT
    GGACAGATTC TGAAGGATGA AAAAGAATTA ACCTTCTATT ATTTGATCAA AGCAAGAGGA
    ATGATCTCCC GATCAGGAAT CCATGTGCTG TCCATTGAAC AAGGAGACTT GAAAGGGGAA
    TTTTCCTTCT CCTTTCGAGT GGAATCAGAC ATTGCTCCTG CAGCCCAGCT GCTCATTTAT
    GCTATTTTGC CCAATGGAGA AATTGTTGCC GATACTGAGA AACTCGAGAT TGAAAACTGT
    TTTGCCAATA AGGTAAATTT GAGCTTCTCC TCAAACCAGA GCCTGCCGGC CTCACACACC
    AACCTAGAGG TCAGGGCCTT TCCTAACTCC CTCTGTGCCC TCCGAGCAGT AGACCAGAGT
    GTTCTGCTGA TGAAGCCTGA AACTGAGCTC TCCCCTCAGT CGGTCTATAA TTTGCTACCA
    GTAAAGGGTA TCTTTAGTGT TGGTTATGGA GATCTCAGAA GAGAAGATAG TGAGCAATGT
    ATCCACGCAG AAGACTTTAT TCACGAGGGG ATCATTTACA CGCCAAAGCA GATCCTGGAT
    AATGATGACA TTTACAGTAT TTTTGAGTCC GCAGGATTAT ATATTTTTAC CAACTCAAAA
    ATCCACAAGC CGAATTTTTG TCAGCTGCCA CAGCCATATC CGGGAGTGCC TATGGCCCAT
    GCAGCAGTTA ACGGTGAGAT TTATTTGCCA GTCCTGCCTC TAGCACGTGG TAGTGCAGGA
    GCAGTTTATC CAATGGTCAT GAGACACAGT TCTCCAGCCG TGGAAGTGAA AGAGACAGTG
    CGGAAGTACT TCCCTGAGAC CTGGATCTGG GACTTGGTAC CAGTCGATTT ATCAGGTAGA
    AGTGAGCTGG CAGTGAAGGT CCCTGACACC ATCACAGAGT GGAAGGCCAG TGCCCTCTGC
    TTGTCAGAAA TGACAGGGCT AGGCCTCTCC CCCACCATCT CTCTTCAAGT GTTCCAGCCT
    TTTTTCCTGG AACTCACTCT TCCCTACTCT GTGGTGCGAG GAGAAGCCTT CACACTCAAA
    GCAACCGTGT TCAACTATTT GTCCCACTGC ATTCGGGTCA GTGTGCAGCT GGAAGCCTCT
    CCTGACAAGT CGGCTGACCT AGAGAGAAAG AACGAAGAAA CTCCCTGTAT CTGTGGAAAT
    CACCAGAAAA CTATGTTCTG GGCTGTGACC CCAAAGTCAC TGGGAAAGAT GAATTTCACA
    GCTACTGTGG AAGCCTTGCA GTCTCAGGAA TTGTGTGGCA ATGAGGTGCC AAGAGTCCCA
    GAACTTGGAC AGAAGGATAC TGTAGTCAAG CCCTTATTAG TTGAGCCTGA AGGAATAGAA
    AAGGAGGAAA CTTTCAACAC ACTCCTCTGT GCAGCAGAAA CTGATGTACC TGAAAAGTTG
    TCACTAAAAG TTCCTTCAGA TGTGGTTGAA GGATCTGCCA GGGCGACATA TTCGGTTTTG
    GGTGACATTC TAGGCTCTGC AATGCAAAAT CTTCAGAATC TTCTCCGGAT GCCATATGGT
    TGTGGAGAGC AAAATATGGT CCTATTTGTC CCTAACATCT ATGTTCTGAA CTACCTGAAT
    GAGACACGAC AACTGACAGA GACTATCAAG TCCAAAGCCA TCAACTACCT CATCAGTGGG
    TACCAAAGGC AGTTGAATTA TAAGCACAGT GATGGTTCAT ACAGCACCTT TGGGGATCAT
    GATGGAAGAA GTCAGGGAAA CACTTGGCTC ACTGCATTTG TGCTCAAGTC TTTTGCTCAA
    GCCCGGTCAC ACATTTTTGT GGAGATGTCA CACATCACGA GTTCCCTCAC CTGGCTCTCA
    CAAAGACAGA AGGAAAATGG TTGTTTCCAA CGCTCTGGAT CACTACTAAA CAATGCTGTT
    AAGGGTGGGG TAGATGATGA ATTGACGCTC TCTGCCTACA TCACCATTGC TCTGCTGGAG
    ATGCCACTGC CTGTCACTCA CCCTCTTGTC CGCAATGCTC TATTCTGCCT GGAAAGTGCC
    TGGGGATCCA TCTCAGAGGC CCAAGGAAGT CTTGTCTATA CGAAGGCATT ATTGGCTTAT
    GCCTTTGCCC TAGCAGGAAA CCAGGTCAAG CGAAATGAGC TTCTTAAATC ACTAGACAAA
    GATGCTGTGA AAGAAGAGGA CTCAATCCAC TGGCAGCGTC CTGGGAAAGT TGAAGAAGTT
    ACGACATTCT ATCAACCCCG GGCTCCTTCA GTTGAAGTGG AGATGACCTC TTACCTGCTC
    CTTGCCTATC TTACTGCTTC GCCTGCCCCA TCTTCAGAAG ACCTGTCTGT GGCTTCACGT
    ATTGTGAAAT GGATCACCAA GCAACAAAAC GCCAATGGGG GCTTCTCTTC CACTCAGGAT
    ACAGTGGTAG CTCTCCAAGC CCTGTCCAAA TATGGAGCAG CAACTTTCAG TAAAAGGGAG
    AAAACAACTG TAGTCACTGT CAAGTCTTCA GAGACATTCT CCCAAAAATT CGAAGTACAC
    GATGCCAATC GCCTGTTGCT ACAAGAAGTC AGGTTGCCAG AGATTCCAGG GGAGTACAGC
    ACAACAGTGT CAGGATCAGG ATGTGTGTAC CTCCAGACAT CCCTGAGATA CAACATCCTG
    CCCAAGAAAG AAGGCAAAGT TCCCTTCAAT CTGAAGGTTG ATACTTTCCC CAAGAATTGT
    GATGGAGTAG ACACTCACAG GAAATTCCAG ATTCACATCA ACATTAGTTA TACTGGGGAA
    CGATCAAGCT CCAACATGGT CATTGTTGAC GTGAAGATGG TATCAGGCTT CATACCCGTG
    AAATCATCAG TGAGAAAGCT CCAAGAAAAG CCTCAGATTC AAAGGACTGA AGTGAGCACC
    AACCATGTTC TGATTTACTT TGAAGAGCTA ACCAATCAAA CTCTGAGTTT CTCCTTCTCA
    GTGGAACAAG ACATCCAAGT TGACAATTTA AAACCAGCTA CTGTAAAAGC CTATGACTAT
    TATGAGACAG AGGAATTTAC TATTGAAGAG TACAGGGATC CCTGCAGTGC TGAATCTGGA
    CACAGAACTG TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27344
    Clone ID Related Accession (Same CDS sequence) XM_005637173.3 , XM_005637173.2
    Accession Version XM_005637173.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4455bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product alpha-2-macroglobulin-like isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005637173.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGGAGAA ATCCACTGCC CATCCCAGTC TTGATTCTGT TTTTCCTGCT CCTTCCCAGA 
    GATGCTTCCA CTACTTCAAA ACCGCAGTAC CTAGTGCTGG TCCCATCACA GCTGTACGCT
    AAGGTTCCTG AGAAGGCCTG TGTTATGCTT AATGACCTGA ATGAGACGGT GACAGTAAAC
    TTAATTCTGG AGTATGCAAT GCAAACCAGA ACCCTCCTCT CAGACGTGGT GACAAAGAAT
    TCCTTCTACT GCAGGCCCTT CACGATTCCA GAGTTGCCGT CGTCCCCAGG GCTCATTACC
    GTGGAGGTGA AGGGGCCAAC CCAACTGTTC ATAAAGAAGA AGAAAGTACG CATCACAAAA
    GTAAACAGCC TGATCTTTGT CCAAACAGAC AAACCCATCT ACAAGCCAGG ACAAACAGTG
    AGATTCCGTG TTGTCTCTGT GGATGTCAGT TTTCATCCTT TGAATGAAAC CTTGCCGGTG
    GTTTATATTG AGAATCCCAA AAGGAACCGA ATTTTCCAAT GGCAAAATCT CAGATTGCAA
    GGAGGACTCA GCCAGCTCTC CTTCCCACTC TCAGTGGAGC CCACCCTGGG TGCTTATAAG
    GTCAAGCTGG AGAAGGAGTC AGGGGAAAAA ATAGAGCACC CCTTTGAAGT GAAGGAATAC
    GTTTTGCCCA AATTTGAGGT CCAAGTGAAA ATGCCCAAGA CGATTAGTTT TCTGGATGAA
    GAATTTGTAG TATCCGCCTG TGGCCTATAC ACGTATGGAA AGCCTGTCTC TGGTGTGGTG
    ACAATTAACA TATGCAGAAA ATACTCACAA TATCATTTTG CCTGCCATGG AATGCATTCG
    CAAGGTGTCT GTGAAGAATT CAGTCAACAG GCAGATGATA AAGGCTGTTT CACACAACTC
    GTGAAGACCA AAGTATTTCA GCTGAGACAA AGGGGGTATG ACATGACAAT ACAAGTGGAA
    GCCAAAATCA AAGAGGAGGG AACAGAGGTG GAACTAACTG GGCATGGATC ATGTGAAATC
    ACAAATATCC TGAGCAAGCT GAAATTTACG AAAGCGGATA CATATTATAG ACCTGGGCTT
    CCTTTTTATG GGCAGGTTCT TCTAGTTGAC GAGAAGGATC AGCCAATGGC CCATAAAACT
    GTAGTCGTCA GTGTGGACGT AACCACATAC CAATCCAGCC TTACCACGAA TGAACATGGT
    TTGGTGAACA TTTACATTGA TACCACCAAC TTCACATCTT CTCTCTTTAC AGTTATGGTG
    ACTTACAAAC AGAATGTCCA CTGTTTTGAT AATTGGTGGC TTGAAGAACT TCATAACCAA
    GCACAGCACA CTGTAAAGCA TATTTTCTCC CCGAGCAAGA GTTATGTTCA CCTTGAACTT
    GTTGCTGGTA CCATAACCTG TGGACAAACC CAGGAGATCC GGGCGCACTA CATCCTGAAT
    GGACAGATTC TGAAGGATGA AAAAGAATTA ACCTTCTATT ATTTGATCAA AGCAAGAGGA
    ATGATCTCCC GATCAGGAAT CCATGTGCTG TCCATTGAAC AAGGAGACTT GAAAGGGGAA
    TTTTCCTTCT CCTTTCGAGT GGAATCAGAC ATTGCTCCTG CAGCCCAGCT GCTCATTTAT
    GCTATTTTGC CCAATGGAGA AATTGTTGCC GATACTGAGA AACTCGAGAT TGAAAACTGT
    TTTGCCAATA AGGTAAATTT GAGCTTCTCC TCAAACCAGA GCCTGCCGGC CTCACACACC
    AACCTAGAGG TCAGGGCCTT TCCTAACTCC CTCTGTGCCC TCCGAGCAGT AGACCAGAGT
    GTTCTGCTGA TGAAGCCTGA AACTGAGCTC TCCCCTCAGT CGGTCTATAA TTTGCTACCA
    GTAAAGGGTA TCTTTAGTGT TGGTTATGGA GATCTCAGAA GAGAAGATAG TGAGCAATGT
    ATCCACGCAG AAGACTTTAT TCACGAGGGG ATCATTTACA CGCCAAAGCA GATCCTGGAT
    AATGATGACA TTTACAGTAT TTTTGAGTCC GCAGGATTAT ATATTTTTAC CAACTCAAAA
    ATCCACAAGC CGAATTTTTG TCAGCTGCCA CAGCCATATC CGGGAGTGCC TATGGCCCAT
    GCAGCAGTTA ACGGTGAGAT TTATTTGCCA GTCCTGCCTC TAGCACGTGG TAGTGCAGGA
    GCAGTTTATC CAATGGTCAT GAGACACAGT TCTCCAGCCG TGGAAGTGAA AGAGACAGTG
    CGGAAGTACT TCCCTGAGAC CTGGATCTGG GACTTGGTAC CAGTCGATTT ATCAGGTAGA
    AGTGAGCTGG CAGTGAAGGT CCCTGACACC ATCACAGAGT GGAAGGCCAG TGCCCTCTGC
    TTGTCAGAAA TGACAGGGCT AGGCCTCTCC CCCACCATCT CTCTTCAAGT GTTCCAGCCT
    TTTTTCCTGG AACTCACTCT TCCCTACTCT GTGGTGCGAG GAGAAGCCTT CACACTCAAA
    GCAACCGTGT TCAACTATTT GTCCCACTGC ATTCGGGTCA GTGTGCAGCT GGAAGCCTCT
    CCTGACAAGT CGGCTGACCT AGAGAGAAAG AACGAAGAAA CTCCCTGTAT CTGTGGAAAT
    CACCAGAAAA CTATGTTCTG GGCTGTGACC CCAAAGTCAC TGGGAAAGAT GAATTTCACA
    GCTACTGTGG AAGCCTTGCA GTCTCAGGAA TTGTGTGGCA ATGAGGTGCC AAGAGTCCCA
    GAACTTGGAC AGAAGGATAC TGTAGTCAAG CCCTTATTAG TTGAGCCTGA AGGAATAGAA
    AAGGAGGAAA CTTTCAACAC ACTCCTCTGT GCAGCAGAAA CTGATGTACC TGAAAAGTTG
    TCACTAAAAG TTCCTTCAGA TGTGGTTGAA GGATCTGCCA GGGCGACATA TTCGGTTTTG
    GGTGACATTC TAGGCTCTGC AATGCAAAAT CTTCAGAATC TTCTCCGGAT GCCATATGGT
    TGTGGAGAGC AAAATATGGT CCTATTTGTC CCTAACATCT ATGTTCTGAA CTACCTGAAT
    GAGACACGAC AACTGACAGA GACTATCAAG TCCAAAGCCA TCAACTACCT CATCAGTGGG
    TACCAAAGGC AGTTGAATTA TAAGCACAGT GATGGTTCAT ACAGCACCTT TGGGGATCAT
    GATGGAAGAA GTCAGGGAAA CACTTGGCTC ACTGCATTTG TGCTCAAGTC TTTTGCTCAA
    GCCCGGTCAC ACATTTTTGT GGAGATGTCA CACATCACGA GTTCCCTCAC CTGGCTCTCA
    CAAAGACAGA AGGAAAATGG TTGTTTCCAA CGCTCTGGAT CACTACTAAA CAATGCTGTT
    AAGGGTGGGG TAGATGATGA ATTGACGCTC TCTGCCTACA TCACCATTGC TCTGCTGGAG
    ATGCCACTGC CTGTCACTCA CCCTCTTGTC CGCAATGCTC TATTCTGCCT GGAAAGTGCC
    TGGGGATCCA TCTCAGAGGC CCAAGGAAGT CTTGTCTATA CGAAGGCATT ATTGGCTTAT
    GCCTTTGCCC TAGCAGGAAA CCAGGTCAAG CGAAATGAGC TTCTTAAATC ACTAGACAAA
    GATGCTGTGA AAGAAGAGGA CTCAATCCAC TGGCAGCGTC CTGGGAAAGT TGAAGAAGTT
    ACGACATTCT ATCAACCCCG GGCTCCTTCA GTTGAAGTGG AGATGACCTC TTACCTGCTC
    CTTGCCTATC TTACTGCTTC GCCTGCCCCA TCTTCAGAAG ACCTGTCTGT GGCTTCACGT
    ATTGTGAAAT GGATCACCAA GCAACAAAAC GCCAATGGGG GCTTCTCTTC CACTCAGGAT
    ACAGTGGTAG CTCTCCAAGC CCTGTCCAAA TATGGAGCAG CAACTTTCAG TAAAAGGGAG
    AAAACAACTG TAGTCACTGT CAAGTCTTCA GAGACATTCT CCCAAAAATT CGAAGTACAC
    GATGCCAATC GCCTGTTGCT ACAAGAAGTC AGGTTGCCAG AGATTCCAGG GGAGTACAGC
    ACAACAGTGT CAGGATCAGG ATGTGTGTAC CTCCAGACAT CCCTGAGATA CAACATCCTG
    CCCAAGAAAG AAGGCAAAGT TCCCTTCAAT CTGAAGGTTG ATACTTTCCC CAAGAATTGT
    GATGGAGTAG ACACTCACAG GAAATTCCAG ATTCACATCA ACATTAGTTA TACTGGGGAA
    CGATCAAGCT CCAACATGGT CATTGTTGAC GTGAAGATGG TATCAGGCTT CATACCCGTG
    AAATCATCAG TGAGAAAGCT CCAAGAAAAG CCTCAGATTC AAAGGACTGA AGTGAGCACC
    AACCATGTTC TGATTTACTT TGAAGAGCTA ACCAATCAAA CTCTGAGTTT CTCCTTCTCA
    GTGGAACAAG ACATCCAAGT TGACAATTTA AAACCAGCTA CTGTAAAAGC CTATGACTAT
    TATGAGACAG AGGAATTTAC TATTGAAGAG TACAGGGATC CCTGCAGTGC TGAATCTGGA
    CACAGAACTG TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005637230.1
    CDS85..4539
    Translation

    Target ORF information:

    RefSeq Version XM_005637173.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris alpha-2-macroglobulin-like (LOC611458), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005637173.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGGAGAA ATCCACTGCC CATCCCAGTC TTGATTCTGT TTTTCCTGCT CCTTCCCAGA 
    GATGCTTCCA CTACTTCAAA ACCGCAGTAC CTAGTGCTGG TCCCATCACA GCTGTACGCT
    AAGGTTCCTG AGAAGGCCTG TGTTATGCTT AATGACCTGA ATGAGACGGT GACAGTAAAC
    TTAATTCTGG AGTATGCAAT GCAAACCAGA ACCCTCCTCT CAGACGTGGT GACAAAGAAT
    TCCTTCTACT GCAGGCCCTT CACGATTCCA GAGTTGCCGT CGTCCCCAGG GCTCATTACC
    GTGGAGGTGA AGGGGCCAAC CCAACTGTTC ATAAAGAAGA AGAAAGTACG CATCACAAAA
    GTAAACAGCC TGATCTTTGT CCAAACAGAC AAACCCATCT ACAAGCCAGG ACAAACAGTG
    AGATTCCGTG TTGTCTCTGT GGATGTCAGT TTTCATCCTT TGAATGAAAC CTTGCCGGTG
    GTTTATATTG AGAATCCCAA AAGGAACCGA ATTTTCCAAT GGCAAAATCT CAGATTGCAA
    GGAGGACTCA GCCAGCTCTC CTTCCCACTC TCAGTGGAGC CCACCCTGGG TGCTTATAAG
    GTCAAGCTGG AGAAGGAGTC AGGGGAAAAA ATAGAGCACC CCTTTGAAGT GAAGGAATAC
    GTTTTGCCCA AATTTGAGGT CCAAGTGAAA ATGCCCAAGA CGATTAGTTT TCTGGATGAA
    GAATTTGTAG TATCCGCCTG TGGCCTATAC ACGTATGGAA AGCCTGTCTC TGGTGTGGTG
    ACAATTAACA TATGCAGAAA ATACTCACAA TATCATTTTG CCTGCCATGG AATGCATTCG
    CAAGGTGTCT GTGAAGAATT CAGTCAACAG GCAGATGATA AAGGCTGTTT CACACAACTC
    GTGAAGACCA AAGTATTTCA GCTGAGACAA AGGGGGTATG ACATGACAAT ACAAGTGGAA
    GCCAAAATCA AAGAGGAGGG AACAGAGGTG GAACTAACTG GGCATGGATC ATGTGAAATC
    ACAAATATCC TGAGCAAGCT GAAATTTACG AAAGCGGATA CATATTATAG ACCTGGGCTT
    CCTTTTTATG GGCAGGTTCT TCTAGTTGAC GAGAAGGATC AGCCAATGGC CCATAAAACT
    GTAGTCGTCA GTGTGGACGT AACCACATAC CAATCCAGCC TTACCACGAA TGAACATGGT
    TTGGTGAACA TTTACATTGA TACCACCAAC TTCACATCTT CTCTCTTTAC AGTTATGGTG
    ACTTACAAAC AGAATGTCCA CTGTTTTGAT AATTGGTGGC TTGAAGAACT TCATAACCAA
    GCACAGCACA CTGTAAAGCA TATTTTCTCC CCGAGCAAGA GTTATGTTCA CCTTGAACTT
    GTTGCTGGTA CCATAACCTG TGGACAAACC CAGGAGATCC GGGCGCACTA CATCCTGAAT
    GGACAGATTC TGAAGGATGA AAAAGAATTA ACCTTCTATT ATTTGATCAA AGCAAGAGGA
    ATGATCTCCC GATCAGGAAT CCATGTGCTG TCCATTGAAC AAGGAGACTT GAAAGGGGAA
    TTTTCCTTCT CCTTTCGAGT GGAATCAGAC ATTGCTCCTG CAGCCCAGCT GCTCATTTAT
    GCTATTTTGC CCAATGGAGA AATTGTTGCC GATACTGAGA AACTCGAGAT TGAAAACTGT
    TTTGCCAATA AGGTAAATTT GAGCTTCTCC TCAAACCAGA GCCTGCCGGC CTCACACACC
    AACCTAGAGG TCAGGGCCTT TCCTAACTCC CTCTGTGCCC TCCGAGCAGT AGACCAGAGT
    GTTCTGCTGA TGAAGCCTGA AACTGAGCTC TCCCCTCAGT CGGTCTATAA TTTGCTACCA
    GTAAAGGGTA TCTTTAGTGT TGGTTATGGA GATCTCAGAA GAGAAGATAG TGAGCAATGT
    ATCCACGCAG AAGACTTTAT TCACGAGGGG ATCATTTACA CGCCAAAGCA GATCCTGGAT
    AATGATGACA TTTACAGTAT TTTTGAGTCC GCAGGATTAT ATATTTTTAC CAACTCAAAA
    ATCCACAAGC CGAATTTTTG TCAGCTGCCA CAGCCATATC CGGGAGTGCC TATGGCCCAT
    GCAGCAGTTA ACGGTGAGAT TTATTTGCCA GTCCTGCCTC TAGCACGTGG TAGTGCAGGA
    GCAGTTTATC CAATGGTCAT GAGACACAGT TCTCCAGCCG TGGAAGTGAA AGAGACAGTG
    CGGAAGTACT TCCCTGAGAC CTGGATCTGG GACTTGGTAC CAGTCGATTT ATCAGGTAGA
    AGTGAGCTGG CAGTGAAGGT CCCTGACACC ATCACAGAGT GGAAGGCCAG TGCCCTCTGC
    TTGTCAGAAA TGACAGGGCT AGGCCTCTCC CCCACCATCT CTCTTCAAGT GTTCCAGCCT
    TTTTTCCTGG AACTCACTCT TCCCTACTCT GTGGTGCGAG GAGAAGCCTT CACACTCAAA
    GCAACCGTGT TCAACTATTT GTCCCACTGC ATTCGGGTCA GTGTGCAGCT GGAAGCCTCT
    CCTGACAAGT CGGCTGACCT AGAGAGAAAG AACGAAGAAA CTCCCTGTAT CTGTGGAAAT
    CACCAGAAAA CTATGTTCTG GGCTGTGACC CCAAAGTCAC TGGGAAAGAT GAATTTCACA
    GCTACTGTGG AAGCCTTGCA GTCTCAGGAA TTGTGTGGCA ATGAGGTGCC AAGAGTCCCA
    GAACTTGGAC AGAAGGATAC TGTAGTCAAG CCCTTATTAG TTGAGCCTGA AGGAATAGAA
    AAGGAGGAAA CTTTCAACAC ACTCCTCTGT GCAGCAGAAA CTGATGTACC TGAAAAGTTG
    TCACTAAAAG TTCCTTCAGA TGTGGTTGAA GGATCTGCCA GGGCGACATA TTCGGTTTTG
    GGTGACATTC TAGGCTCTGC AATGCAAAAT CTTCAGAATC TTCTCCGGAT GCCATATGGT
    TGTGGAGAGC AAAATATGGT CCTATTTGTC CCTAACATCT ATGTTCTGAA CTACCTGAAT
    GAGACACGAC AACTGACAGA GACTATCAAG TCCAAAGCCA TCAACTACCT CATCAGTGGG
    TACCAAAGGC AGTTGAATTA TAAGCACAGT GATGGTTCAT ACAGCACCTT TGGGGATCAT
    GATGGAAGAA GTCAGGGAAA CACTTGGCTC ACTGCATTTG TGCTCAAGTC TTTTGCTCAA
    GCCCGGTCAC ACATTTTTGT GGAGATGTCA CACATCACGA GTTCCCTCAC CTGGCTCTCA
    CAAAGACAGA AGGAAAATGG TTGTTTCCAA CGCTCTGGAT CACTACTAAA CAATGCTGTT
    AAGGGTGGGG TAGATGATGA ATTGACGCTC TCTGCCTACA TCACCATTGC TCTGCTGGAG
    ATGCCACTGC CTGTCACTCA CCCTCTTGTC CGCAATGCTC TATTCTGCCT GGAAAGTGCC
    TGGGGATCCA TCTCAGAGGC CCAAGGAAGT CTTGTCTATA CGAAGGCATT ATTGGCTTAT
    GCCTTTGCCC TAGCAGGAAA CCAGGTCAAG CGAAATGAGC TTCTTAAATC ACTAGACAAA
    GATGCTGTGA AAGAAGAGGA CTCAATCCAC TGGCAGCGTC CTGGGAAAGT TGAAGAAGTT
    ACGACATTCT ATCAACCCCG GGCTCCTTCA GTTGAAGTGG AGATGACCTC TTACCTGCTC
    CTTGCCTATC TTACTGCTTC GCCTGCCCCA TCTTCAGAAG ACCTGTCTGT GGCTTCACGT
    ATTGTGAAAT GGATCACCAA GCAACAAAAC GCCAATGGGG GCTTCTCTTC CACTCAGGAT
    ACAGTGGTAG CTCTCCAAGC CCTGTCCAAA TATGGAGCAG CAACTTTCAG TAAAAGGGAG
    AAAACAACTG TAGTCACTGT CAAGTCTTCA GAGACATTCT CCCAAAAATT CGAAGTACAC
    GATGCCAATC GCCTGTTGCT ACAAGAAGTC AGGTTGCCAG AGATTCCAGG GGAGTACAGC
    ACAACAGTGT CAGGATCAGG ATGTGTGTAC CTCCAGACAT CCCTGAGATA CAACATCCTG
    CCCAAGAAAG AAGGCAAAGT TCCCTTCAAT CTGAAGGTTG ATACTTTCCC CAAGAATTGT
    GATGGAGTAG ACACTCACAG GAAATTCCAG ATTCACATCA ACATTAGTTA TACTGGGGAA
    CGATCAAGCT CCAACATGGT CATTGTTGAC GTGAAGATGG TATCAGGCTT CATACCCGTG
    AAATCATCAG TGAGAAAGCT CCAAGAAAAG CCTCAGATTC AAAGGACTGA AGTGAGCACC
    AACCATGTTC TGATTTACTT TGAAGAGCTA ACCAATCAAA CTCTGAGTTT CTCCTTCTCA
    GTGGAACAAG ACATCCAAGT TGACAATTTA AAACCAGCTA CTGTAAAAGC CTATGACTAT
    TATGAGACAG AGGAATTTAC TATTGAAGAG TACAGGGATC CCTGCAGTGC TGAATCTGGA
    CACAGAACTG TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27345
    Clone ID Related Accession (Same CDS sequence) XM_005637174.3 , XM_005637174.2
    Accession Version XM_005637174.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pregnancy zone protein-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005637174.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAGGAGAA ATCCACTGCC CATCCCAGTC TTGATTCTGT TTTTCCTGCT CCTTCCCAGA 
    GATGCTTCCA CTACTTCAAA ACCGCAGTAC CTAGTGCTGG TCCCATCACA GCTGTACGCT
    AAGGTTCCTG AGAAGGCCTG TGTTATGCTT AATGACCTGA ATGAGACGGT GACAGTAAAC
    TTAATTCTGG AGTATGCAAT GCAAACCAGA ACCCTCCTCT CAGACGTGGT GACAAAGAAT
    TCCTTCTACT GCAGGCCCTT CACGATTCCA GAGTTGCCGT CGTCCCCAGG GCTCATTACC
    GTGGAGGTGA AGGGGCCAAC CCAACTGTTC ATAAAGAAGA AGAAAGTACG CATCACAAAA
    GTAAACAGCC TGATCTTTGT CCAAACAGAC AAACCCATCT ACAAGCCAGG ACAAACAGTG
    AGATTCCGTG TTGTCTCTGT GGATGTCAGT TTTCATCCTT TGAATGAAAC CTTGCCGGTG
    GTTTATATTG AGAATCCCAA AAGGAACCGA ATTTTCCAAT GGCAAAATCT CAGATTGCAA
    GGAGGACTCA GCCAGCTCTC CTTCCCACTC TCAGTGGAGC CCACCCTGGG TGCTTATAAG
    GTCAAGCTGG AGAAGGAGTC AGGGGAAAAA ATAGAGCACC CCTTTGAAGT GAAGGAATAC
    GTTTTGCCCA AATTTGAGGT CCAAGTGAAA ATGCCCAAGA CGATTAGTTT TCTGGATGAA
    GAATTTGTAG TATCCGCCTG TGGCCTATAC ACGTATGGAA AGCCTGTCTC TGGTGTGGTG
    ACAATTAACA TATGCAGAAA ATACTCACAA TATCATTTTG CCTGCCATGG AATGCATTCG
    CAAGGTGTCT GTGAAGAATT CAGTCAACAG GCAGATGATA AAGGCTGTTT CACACAACTC
    GTGAAGACCA AAGTATTTCA GCTGAGACAA AGGGGGTATG ACATGACAAT ACAAGTGGAA
    GCCAAAATCA AAGAGGAGGG AACAGAGGTG GAACTAACTG GGCATGGATC ATGTGAAATC
    ACAAATATCC TGAGCAAGCT GAAATTTACG AAAGCGGATA CATATTATAG ACCTGGGCTT
    CCTTTTTATG GGCAGGTTCT TCTAGTTGAC GAGAAGGATC AGCCAATGGC CCATAAAACT
    GTAGTCGTCA GTGTGGACGT AACCACATAC CAATCCAGCC TTACCACGAA TGAACATGGT
    TTGGTGAACA TTTACATTGA TACCACCAAC TTCACATCTT CTCTCTTTAC AGTTATGGTG
    ACTTACAAAC AGAATGTCCA CTGTTTTGAT AATTGGTGGC TTGAAGAACT TCATAACCAA
    GCACAGCACA CTGTAAAGCA TATTTTCTCC CCGAGCAAGA GTTATGTTCA CCTTGAACTT
    GTTGCTGGTA CCATAACCTG TGGACAAACC CAGGAGATCC GGGCGCACTA CATCCTGAAT
    GGACAGATTC TGAAGGATGA AAAAGAATTA ACCTTCTATT ATTTGATCAA AGCAAGAGGA
    ATGATCTCCC GATCAGGAAT CCATGTGCTG TCCATTGAAC AAGGAGACTT GAAAGGGGAA
    TTTTCCTTCT CCTTTCGAGT GGAATCAGAC ATTGCTCCTG CAGCCCAGCT GCTCATTTAT
    GCTATTTTGC CCAATGGAGA AATTGTTGCC GATACTGAGA AACTCGAGAT TGAAAACTGT
    TTTGCCAATA AGGTAAATTT GAGCTTCTCC TCAAACCAGA GCCTGCCGGC CTCACACACC
    AACCTAGAGG TCAGGGCCTT TCCTAACTCC CTCTGTGCCC TCCGAGCAGT AGACCAGAGT
    GTTCTGCTGA TGAAGCCTGA AACTGAGCTC TCCCCTCAGT CGGTCTATAA TTTGCTACCA
    GTAAAGGGTA TCTTTAGTGT TGGTTATGGA GATCTCAGAA GAGAAGATAG TGAGCAATGT
    ATCCACGCAG AAGACTTTAT TCACGAGGGG ATCATTTACA CGCCAAAGCA GATCCTGGAT
    AATGATGACA TTTACAGTAT TTTTGAGTCC GCAGGATTAT ATATTTTTAC CAACTCAAAA
    ATCCACAAGC CGAATTTTTG TCAGCTGCCA CAGCCATATC CGGGAGTGCC TATGGCCCAT
    GCAGCAGTTA ACGTCCTGCC TCTAGCACGT GGTAGTGCAG GAGCAGTTTA TCCAATGGTC
    ATGAGACACA GTTCTCCAGC CGTGGAAGTG AAAGAGACAG TGCGGAAGTA CTTCCCTGAG
    ACCTGGATCT GGGACTTGGT ACCAGTCGAT TTATCAGGTA GAAGTGAGCT GGCAGTGAAG
    GTCCCTGACA CCATCACAGA GTGGAAGGCC AGTGCCCTCT GCTTGTCAGA AATGACAGGG
    CTAGGCCTCT CCCCCACCAT CTCTCTTCAA GTGTTCCAGC CTTTTTTCCT GGAACTCACT
    CTTCCCTACT CTGTGGTGCG AGGAGAAGCC TTCACACTCA AAGCAACCGT GTTCAACTAT
    TTGTCCCACT GCATTCGGGT CAGTGTGCAG CTGGAAGCCT CTCCTGACAA GTCGGCTGAC
    CTAGAGAGAA AGAACGAAGA AACTCCCTGT ATCTGTGGAA ATCACCAGAA AACTATGTTC
    TGGGCTGTGA CCCCAAAGTC ACTGGGAAAG ATGAATTTCA CAGCTACTGT GGAAGCCTTG
    CAGTCTCAGG AATTGTGTGG CAATGAGGTG CCAAGAGTCC CAGAACTTGG ACAGAAGGAT
    ACTGTAGTCA AGCCCTTATT AGTTGAGCCT GAAGGAATAG AAAAGGAGGA AACTTTCAAC
    ACACTCCTCT GTGCAGCAGA AACTGATGTA CCTGAAAAGT TGTCACTAAA AGTTCCTTCA
    GATGTGGTTG AAGGATCTGC CAGGGCGACA TATTCGGTTT TGGGTGACAT TCTAGGCTCT
    GCAATGCAAA ATCTTCAGAA TCTTCTCCGG ATGCCATATG GTTGTGGAGA GCAAAATATG
    GTCCTATTTG TCCCTAACAT CTATGTTCTG AACTACCTGA ATGAGACACG ACAACTGACA
    GAGACTATCA AGTCCAAAGC CATCAACTAC CTCATCAGTG GGTACCAAAG GCAGTTGAAT
    TATAAGCACA GTGATGGTTC ATACAGCACC TTTGGGGATC ATGATGGAAG AAGTCAGGGA
    AACACTTGGC TCACTGCATT TGTGCTCAAG TCTTTTGCTC AAGCCCGGTC ACACATTTTT
    GTGGAGATGT CACACATCAC GAGTTCCCTC ACCTGGCTCT CACAAAGACA GAAGGAAAAT
    GGTTGTTTCC AACGCTCTGG ATCACTACTA AACAATGCTG TTAAGGGTGG GGTAGATGAT
    GAATTGACGC TCTCTGCCTA CATCACCATT GCTCTGCTGG AGATGCCACT GCCTGTCACT
    CACCCTCTTG TCCGCAATGC TCTATTCTGC CTGGAAAGTG CCTGGGGATC CATCTCAGAG
    GCCCAAGGAA GTCTTGTCTA TACGAAGGCA TTATTGGCTT ATGCCTTTGC CCTAGCAGGA
    AACCAGGTCA AGCGAAATGA GCTTCTTAAA TCACTAGACA AAGATGCTGT GAAAGAAGAG
    GACTCAATCC ACTGGCAGCG TCCTGGGAAA GTTGAAGAAG TTACGACATT CTATCAACCC
    CGGGCTCCTT CAGTTGAAGT GGAGATGACC TCTTACCTGC TCCTTGCCTA TCTTACTGCT
    TCGCCTGCCC CATCTTCAGA AGACCTGTCT GTGGCTTCAC GTATTGTGAA ATGGATCACC
    AAGCAACAAA ACGCCAATGG GGGCTTCTCT TCCACTCAGG ATACAGTGGT AGCTCTCCAA
    GCCCTGTCCA AATATGGAGC AGCAACTTTC AGTAAAAGGG AGAAAACAAC TGTAGTCACT
    GTCAAGTCTT CAGAGACATT CTCCCAAAAA TTCGAAGTAC ACGATGCCAA TCGCCTGTTG
    CTACAAGAAG TCAGGTTGCC AGAGATTCCA GGGGAGTACA GCACAACAGT GTCAGGATCA
    GGATGTGTGT ACCTCCAGAC ATCCCTGAGA TACAACATCC TGCCCAAGAA AGAAGGCAAA
    GTTCCCTTCA ATCTGAAGGT TGATACTTTC CCCAAGAATT GTGATGGAGT AGACACTCAC
    AGGAAATTCC AGATTCACAT CAACATTAGT TATACTGGGG AACGATCAAG CTCCAACATG
    GTCATTGTTG ACGTGAAGAT GGTATCAGGC TTCATACCCG TGAAATCATC AGTGAGAAAG
    CTCCAAGAAA AGCCTCAGAT TCAAAGGACT GAAGTGAGCA CCAACCATGT TCTGATTTAC
    TTTGAAGAGC TAACCAATCA AACTCTGAGT TTCTCCTTCT CAGTGGAACA AGACATCCAA
    GTTGACAATT TAAAACCAGC TACTGTAAAA GCCTATGACT ATTATGAGAC AGAGGAATTT
    ACTATTGAAG AGTACAGGGA TCCCTGCAGT GCTGAATCTG GACACAGAAC TGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005637231.1
    CDS81..4517
    Translation

    Target ORF information:

    RefSeq Version XM_005637174.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pregnancy zone protein-like (LOC611458), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005637174.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAGGAGAA ATCCACTGCC CATCCCAGTC TTGATTCTGT TTTTCCTGCT CCTTCCCAGA 
    GATGCTTCCA CTACTTCAAA ACCGCAGTAC CTAGTGCTGG TCCCATCACA GCTGTACGCT
    AAGGTTCCTG AGAAGGCCTG TGTTATGCTT AATGACCTGA ATGAGACGGT GACAGTAAAC
    TTAATTCTGG AGTATGCAAT GCAAACCAGA ACCCTCCTCT CAGACGTGGT GACAAAGAAT
    TCCTTCTACT GCAGGCCCTT CACGATTCCA GAGTTGCCGT CGTCCCCAGG GCTCATTACC
    GTGGAGGTGA AGGGGCCAAC CCAACTGTTC ATAAAGAAGA AGAAAGTACG CATCACAAAA
    GTAAACAGCC TGATCTTTGT CCAAACAGAC AAACCCATCT ACAAGCCAGG ACAAACAGTG
    AGATTCCGTG TTGTCTCTGT GGATGTCAGT TTTCATCCTT TGAATGAAAC CTTGCCGGTG
    GTTTATATTG AGAATCCCAA AAGGAACCGA ATTTTCCAAT GGCAAAATCT CAGATTGCAA
    GGAGGACTCA GCCAGCTCTC CTTCCCACTC TCAGTGGAGC CCACCCTGGG TGCTTATAAG
    GTCAAGCTGG AGAAGGAGTC AGGGGAAAAA ATAGAGCACC CCTTTGAAGT GAAGGAATAC
    GTTTTGCCCA AATTTGAGGT CCAAGTGAAA ATGCCCAAGA CGATTAGTTT TCTGGATGAA
    GAATTTGTAG TATCCGCCTG TGGCCTATAC ACGTATGGAA AGCCTGTCTC TGGTGTGGTG
    ACAATTAACA TATGCAGAAA ATACTCACAA TATCATTTTG CCTGCCATGG AATGCATTCG
    CAAGGTGTCT GTGAAGAATT CAGTCAACAG GCAGATGATA AAGGCTGTTT CACACAACTC
    GTGAAGACCA AAGTATTTCA GCTGAGACAA AGGGGGTATG ACATGACAAT ACAAGTGGAA
    GCCAAAATCA AAGAGGAGGG AACAGAGGTG GAACTAACTG GGCATGGATC ATGTGAAATC
    ACAAATATCC TGAGCAAGCT GAAATTTACG AAAGCGGATA CATATTATAG ACCTGGGCTT
    CCTTTTTATG GGCAGGTTCT TCTAGTTGAC GAGAAGGATC AGCCAATGGC CCATAAAACT
    GTAGTCGTCA GTGTGGACGT AACCACATAC CAATCCAGCC TTACCACGAA TGAACATGGT
    TTGGTGAACA TTTACATTGA TACCACCAAC TTCACATCTT CTCTCTTTAC AGTTATGGTG
    ACTTACAAAC AGAATGTCCA CTGTTTTGAT AATTGGTGGC TTGAAGAACT TCATAACCAA
    GCACAGCACA CTGTAAAGCA TATTTTCTCC CCGAGCAAGA GTTATGTTCA CCTTGAACTT
    GTTGCTGGTA CCATAACCTG TGGACAAACC CAGGAGATCC GGGCGCACTA CATCCTGAAT
    GGACAGATTC TGAAGGATGA AAAAGAATTA ACCTTCTATT ATTTGATCAA AGCAAGAGGA
    ATGATCTCCC GATCAGGAAT CCATGTGCTG TCCATTGAAC AAGGAGACTT GAAAGGGGAA
    TTTTCCTTCT CCTTTCGAGT GGAATCAGAC ATTGCTCCTG CAGCCCAGCT GCTCATTTAT
    GCTATTTTGC CCAATGGAGA AATTGTTGCC GATACTGAGA AACTCGAGAT TGAAAACTGT
    TTTGCCAATA AGGTAAATTT GAGCTTCTCC TCAAACCAGA GCCTGCCGGC CTCACACACC
    AACCTAGAGG TCAGGGCCTT TCCTAACTCC CTCTGTGCCC TCCGAGCAGT AGACCAGAGT
    GTTCTGCTGA TGAAGCCTGA AACTGAGCTC TCCCCTCAGT CGGTCTATAA TTTGCTACCA
    GTAAAGGGTA TCTTTAGTGT TGGTTATGGA GATCTCAGAA GAGAAGATAG TGAGCAATGT
    ATCCACGCAG AAGACTTTAT TCACGAGGGG ATCATTTACA CGCCAAAGCA GATCCTGGAT
    AATGATGACA TTTACAGTAT TTTTGAGTCC GCAGGATTAT ATATTTTTAC CAACTCAAAA
    ATCCACAAGC CGAATTTTTG TCAGCTGCCA CAGCCATATC CGGGAGTGCC TATGGCCCAT
    GCAGCAGTTA ACGTCCTGCC TCTAGCACGT GGTAGTGCAG GAGCAGTTTA TCCAATGGTC
    ATGAGACACA GTTCTCCAGC CGTGGAAGTG AAAGAGACAG TGCGGAAGTA CTTCCCTGAG
    ACCTGGATCT GGGACTTGGT ACCAGTCGAT TTATCAGGTA GAAGTGAGCT GGCAGTGAAG
    GTCCCTGACA CCATCACAGA GTGGAAGGCC AGTGCCCTCT GCTTGTCAGA AATGACAGGG
    CTAGGCCTCT CCCCCACCAT CTCTCTTCAA GTGTTCCAGC CTTTTTTCCT GGAACTCACT
    CTTCCCTACT CTGTGGTGCG AGGAGAAGCC TTCACACTCA AAGCAACCGT GTTCAACTAT
    TTGTCCCACT GCATTCGGGT CAGTGTGCAG CTGGAAGCCT CTCCTGACAA GTCGGCTGAC
    CTAGAGAGAA AGAACGAAGA AACTCCCTGT ATCTGTGGAA ATCACCAGAA AACTATGTTC
    TGGGCTGTGA CCCCAAAGTC ACTGGGAAAG ATGAATTTCA CAGCTACTGT GGAAGCCTTG
    CAGTCTCAGG AATTGTGTGG CAATGAGGTG CCAAGAGTCC CAGAACTTGG ACAGAAGGAT
    ACTGTAGTCA AGCCCTTATT AGTTGAGCCT GAAGGAATAG AAAAGGAGGA AACTTTCAAC
    ACACTCCTCT GTGCAGCAGA AACTGATGTA CCTGAAAAGT TGTCACTAAA AGTTCCTTCA
    GATGTGGTTG AAGGATCTGC CAGGGCGACA TATTCGGTTT TGGGTGACAT TCTAGGCTCT
    GCAATGCAAA ATCTTCAGAA TCTTCTCCGG ATGCCATATG GTTGTGGAGA GCAAAATATG
    GTCCTATTTG TCCCTAACAT CTATGTTCTG AACTACCTGA ATGAGACACG ACAACTGACA
    GAGACTATCA AGTCCAAAGC CATCAACTAC CTCATCAGTG GGTACCAAAG GCAGTTGAAT
    TATAAGCACA GTGATGGTTC ATACAGCACC TTTGGGGATC ATGATGGAAG AAGTCAGGGA
    AACACTTGGC TCACTGCATT TGTGCTCAAG TCTTTTGCTC AAGCCCGGTC ACACATTTTT
    GTGGAGATGT CACACATCAC GAGTTCCCTC ACCTGGCTCT CACAAAGACA GAAGGAAAAT
    GGTTGTTTCC AACGCTCTGG ATCACTACTA AACAATGCTG TTAAGGGTGG GGTAGATGAT
    GAATTGACGC TCTCTGCCTA CATCACCATT GCTCTGCTGG AGATGCCACT GCCTGTCACT
    CACCCTCTTG TCCGCAATGC TCTATTCTGC CTGGAAAGTG CCTGGGGATC CATCTCAGAG
    GCCCAAGGAA GTCTTGTCTA TACGAAGGCA TTATTGGCTT ATGCCTTTGC CCTAGCAGGA
    AACCAGGTCA AGCGAAATGA GCTTCTTAAA TCACTAGACA AAGATGCTGT GAAAGAAGAG
    GACTCAATCC ACTGGCAGCG TCCTGGGAAA GTTGAAGAAG TTACGACATT CTATCAACCC
    CGGGCTCCTT CAGTTGAAGT GGAGATGACC TCTTACCTGC TCCTTGCCTA TCTTACTGCT
    TCGCCTGCCC CATCTTCAGA AGACCTGTCT GTGGCTTCAC GTATTGTGAA ATGGATCACC
    AAGCAACAAA ACGCCAATGG GGGCTTCTCT TCCACTCAGG ATACAGTGGT AGCTCTCCAA
    GCCCTGTCCA AATATGGAGC AGCAACTTTC AGTAAAAGGG AGAAAACAAC TGTAGTCACT
    GTCAAGTCTT CAGAGACATT CTCCCAAAAA TTCGAAGTAC ACGATGCCAA TCGCCTGTTG
    CTACAAGAAG TCAGGTTGCC AGAGATTCCA GGGGAGTACA GCACAACAGT GTCAGGATCA
    GGATGTGTGT ACCTCCAGAC ATCCCTGAGA TACAACATCC TGCCCAAGAA AGAAGGCAAA
    GTTCCCTTCA ATCTGAAGGT TGATACTTTC CCCAAGAATT GTGATGGAGT AGACACTCAC
    AGGAAATTCC AGATTCACAT CAACATTAGT TATACTGGGG AACGATCAAG CTCCAACATG
    GTCATTGTTG ACGTGAAGAT GGTATCAGGC TTCATACCCG TGAAATCATC AGTGAGAAAG
    CTCCAAGAAA AGCCTCAGAT TCAAAGGACT GAAGTGAGCA CCAACCATGT TCTGATTTAC
    TTTGAAGAGC TAACCAATCA AACTCTGAGT TTCTCCTTCT CAGTGGAACA AGACATCCAA
    GTTGACAATT TAAAACCAGC TACTGTAAAA GCCTATGACT ATTATGAGAC AGAGGAATTT
    ACTATTGAAG AGTACAGGGA TCCCTGCAGT GCTGAATCTG GACACAGAAC TGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27345
    Clone ID Related Accession (Same CDS sequence) XM_005637174.3 , XM_005637174.2
    Accession Version XM_005637174.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product alpha-2-macroglobulin-like isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005637174.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAGGAGAA ATCCACTGCC CATCCCAGTC TTGATTCTGT TTTTCCTGCT CCTTCCCAGA 
    GATGCTTCCA CTACTTCAAA ACCGCAGTAC CTAGTGCTGG TCCCATCACA GCTGTACGCT
    AAGGTTCCTG AGAAGGCCTG TGTTATGCTT AATGACCTGA ATGAGACGGT GACAGTAAAC
    TTAATTCTGG AGTATGCAAT GCAAACCAGA ACCCTCCTCT CAGACGTGGT GACAAAGAAT
    TCCTTCTACT GCAGGCCCTT CACGATTCCA GAGTTGCCGT CGTCCCCAGG GCTCATTACC
    GTGGAGGTGA AGGGGCCAAC CCAACTGTTC ATAAAGAAGA AGAAAGTACG CATCACAAAA
    GTAAACAGCC TGATCTTTGT CCAAACAGAC AAACCCATCT ACAAGCCAGG ACAAACAGTG
    AGATTCCGTG TTGTCTCTGT GGATGTCAGT TTTCATCCTT TGAATGAAAC CTTGCCGGTG
    GTTTATATTG AGAATCCCAA AAGGAACCGA ATTTTCCAAT GGCAAAATCT CAGATTGCAA
    GGAGGACTCA GCCAGCTCTC CTTCCCACTC TCAGTGGAGC CCACCCTGGG TGCTTATAAG
    GTCAAGCTGG AGAAGGAGTC AGGGGAAAAA ATAGAGCACC CCTTTGAAGT GAAGGAATAC
    GTTTTGCCCA AATTTGAGGT CCAAGTGAAA ATGCCCAAGA CGATTAGTTT TCTGGATGAA
    GAATTTGTAG TATCCGCCTG TGGCCTATAC ACGTATGGAA AGCCTGTCTC TGGTGTGGTG
    ACAATTAACA TATGCAGAAA ATACTCACAA TATCATTTTG CCTGCCATGG AATGCATTCG
    CAAGGTGTCT GTGAAGAATT CAGTCAACAG GCAGATGATA AAGGCTGTTT CACACAACTC
    GTGAAGACCA AAGTATTTCA GCTGAGACAA AGGGGGTATG ACATGACAAT ACAAGTGGAA
    GCCAAAATCA AAGAGGAGGG AACAGAGGTG GAACTAACTG GGCATGGATC ATGTGAAATC
    ACAAATATCC TGAGCAAGCT GAAATTTACG AAAGCGGATA CATATTATAG ACCTGGGCTT
    CCTTTTTATG GGCAGGTTCT TCTAGTTGAC GAGAAGGATC AGCCAATGGC CCATAAAACT
    GTAGTCGTCA GTGTGGACGT AACCACATAC CAATCCAGCC TTACCACGAA TGAACATGGT
    TTGGTGAACA TTTACATTGA TACCACCAAC TTCACATCTT CTCTCTTTAC AGTTATGGTG
    ACTTACAAAC AGAATGTCCA CTGTTTTGAT AATTGGTGGC TTGAAGAACT TCATAACCAA
    GCACAGCACA CTGTAAAGCA TATTTTCTCC CCGAGCAAGA GTTATGTTCA CCTTGAACTT
    GTTGCTGGTA CCATAACCTG TGGACAAACC CAGGAGATCC GGGCGCACTA CATCCTGAAT
    GGACAGATTC TGAAGGATGA AAAAGAATTA ACCTTCTATT ATTTGATCAA AGCAAGAGGA
    ATGATCTCCC GATCAGGAAT CCATGTGCTG TCCATTGAAC AAGGAGACTT GAAAGGGGAA
    TTTTCCTTCT CCTTTCGAGT GGAATCAGAC ATTGCTCCTG CAGCCCAGCT GCTCATTTAT
    GCTATTTTGC CCAATGGAGA AATTGTTGCC GATACTGAGA AACTCGAGAT TGAAAACTGT
    TTTGCCAATA AGGTAAATTT GAGCTTCTCC TCAAACCAGA GCCTGCCGGC CTCACACACC
    AACCTAGAGG TCAGGGCCTT TCCTAACTCC CTCTGTGCCC TCCGAGCAGT AGACCAGAGT
    GTTCTGCTGA TGAAGCCTGA AACTGAGCTC TCCCCTCAGT CGGTCTATAA TTTGCTACCA
    GTAAAGGGTA TCTTTAGTGT TGGTTATGGA GATCTCAGAA GAGAAGATAG TGAGCAATGT
    ATCCACGCAG AAGACTTTAT TCACGAGGGG ATCATTTACA CGCCAAAGCA GATCCTGGAT
    AATGATGACA TTTACAGTAT TTTTGAGTCC GCAGGATTAT ATATTTTTAC CAACTCAAAA
    ATCCACAAGC CGAATTTTTG TCAGCTGCCA CAGCCATATC CGGGAGTGCC TATGGCCCAT
    GCAGCAGTTA ACGTCCTGCC TCTAGCACGT GGTAGTGCAG GAGCAGTTTA TCCAATGGTC
    ATGAGACACA GTTCTCCAGC CGTGGAAGTG AAAGAGACAG TGCGGAAGTA CTTCCCTGAG
    ACCTGGATCT GGGACTTGGT ACCAGTCGAT TTATCAGGTA GAAGTGAGCT GGCAGTGAAG
    GTCCCTGACA CCATCACAGA GTGGAAGGCC AGTGCCCTCT GCTTGTCAGA AATGACAGGG
    CTAGGCCTCT CCCCCACCAT CTCTCTTCAA GTGTTCCAGC CTTTTTTCCT GGAACTCACT
    CTTCCCTACT CTGTGGTGCG AGGAGAAGCC TTCACACTCA AAGCAACCGT GTTCAACTAT
    TTGTCCCACT GCATTCGGGT CAGTGTGCAG CTGGAAGCCT CTCCTGACAA GTCGGCTGAC
    CTAGAGAGAA AGAACGAAGA AACTCCCTGT ATCTGTGGAA ATCACCAGAA AACTATGTTC
    TGGGCTGTGA CCCCAAAGTC ACTGGGAAAG ATGAATTTCA CAGCTACTGT GGAAGCCTTG
    CAGTCTCAGG AATTGTGTGG CAATGAGGTG CCAAGAGTCC CAGAACTTGG ACAGAAGGAT
    ACTGTAGTCA AGCCCTTATT AGTTGAGCCT GAAGGAATAG AAAAGGAGGA AACTTTCAAC
    ACACTCCTCT GTGCAGCAGA AACTGATGTA CCTGAAAAGT TGTCACTAAA AGTTCCTTCA
    GATGTGGTTG AAGGATCTGC CAGGGCGACA TATTCGGTTT TGGGTGACAT TCTAGGCTCT
    GCAATGCAAA ATCTTCAGAA TCTTCTCCGG ATGCCATATG GTTGTGGAGA GCAAAATATG
    GTCCTATTTG TCCCTAACAT CTATGTTCTG AACTACCTGA ATGAGACACG ACAACTGACA
    GAGACTATCA AGTCCAAAGC CATCAACTAC CTCATCAGTG GGTACCAAAG GCAGTTGAAT
    TATAAGCACA GTGATGGTTC ATACAGCACC TTTGGGGATC ATGATGGAAG AAGTCAGGGA
    AACACTTGGC TCACTGCATT TGTGCTCAAG TCTTTTGCTC AAGCCCGGTC ACACATTTTT
    GTGGAGATGT CACACATCAC GAGTTCCCTC ACCTGGCTCT CACAAAGACA GAAGGAAAAT
    GGTTGTTTCC AACGCTCTGG ATCACTACTA AACAATGCTG TTAAGGGTGG GGTAGATGAT
    GAATTGACGC TCTCTGCCTA CATCACCATT GCTCTGCTGG AGATGCCACT GCCTGTCACT
    CACCCTCTTG TCCGCAATGC TCTATTCTGC CTGGAAAGTG CCTGGGGATC CATCTCAGAG
    GCCCAAGGAA GTCTTGTCTA TACGAAGGCA TTATTGGCTT ATGCCTTTGC CCTAGCAGGA
    AACCAGGTCA AGCGAAATGA GCTTCTTAAA TCACTAGACA AAGATGCTGT GAAAGAAGAG
    GACTCAATCC ACTGGCAGCG TCCTGGGAAA GTTGAAGAAG TTACGACATT CTATCAACCC
    CGGGCTCCTT CAGTTGAAGT GGAGATGACC TCTTACCTGC TCCTTGCCTA TCTTACTGCT
    TCGCCTGCCC CATCTTCAGA AGACCTGTCT GTGGCTTCAC GTATTGTGAA ATGGATCACC
    AAGCAACAAA ACGCCAATGG GGGCTTCTCT TCCACTCAGG ATACAGTGGT AGCTCTCCAA
    GCCCTGTCCA AATATGGAGC AGCAACTTTC AGTAAAAGGG AGAAAACAAC TGTAGTCACT
    GTCAAGTCTT CAGAGACATT CTCCCAAAAA TTCGAAGTAC ACGATGCCAA TCGCCTGTTG
    CTACAAGAAG TCAGGTTGCC AGAGATTCCA GGGGAGTACA GCACAACAGT GTCAGGATCA
    GGATGTGTGT ACCTCCAGAC ATCCCTGAGA TACAACATCC TGCCCAAGAA AGAAGGCAAA
    GTTCCCTTCA ATCTGAAGGT TGATACTTTC CCCAAGAATT GTGATGGAGT AGACACTCAC
    AGGAAATTCC AGATTCACAT CAACATTAGT TATACTGGGG AACGATCAAG CTCCAACATG
    GTCATTGTTG ACGTGAAGAT GGTATCAGGC TTCATACCCG TGAAATCATC AGTGAGAAAG
    CTCCAAGAAA AGCCTCAGAT TCAAAGGACT GAAGTGAGCA CCAACCATGT TCTGATTTAC
    TTTGAAGAGC TAACCAATCA AACTCTGAGT TTCTCCTTCT CAGTGGAACA AGACATCCAA
    GTTGACAATT TAAAACCAGC TACTGTAAAA GCCTATGACT ATTATGAGAC AGAGGAATTT
    ACTATTGAAG AGTACAGGGA TCCCTGCAGT GCTGAATCTG GACACAGAAC TGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005637231.1
    CDS85..4521
    Translation

    Target ORF information:

    RefSeq Version XM_005637174.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris alpha-2-macroglobulin-like (LOC611458), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005637174.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAGGAGAA ATCCACTGCC CATCCCAGTC TTGATTCTGT TTTTCCTGCT CCTTCCCAGA 
    GATGCTTCCA CTACTTCAAA ACCGCAGTAC CTAGTGCTGG TCCCATCACA GCTGTACGCT
    AAGGTTCCTG AGAAGGCCTG TGTTATGCTT AATGACCTGA ATGAGACGGT GACAGTAAAC
    TTAATTCTGG AGTATGCAAT GCAAACCAGA ACCCTCCTCT CAGACGTGGT GACAAAGAAT
    TCCTTCTACT GCAGGCCCTT CACGATTCCA GAGTTGCCGT CGTCCCCAGG GCTCATTACC
    GTGGAGGTGA AGGGGCCAAC CCAACTGTTC ATAAAGAAGA AGAAAGTACG CATCACAAAA
    GTAAACAGCC TGATCTTTGT CCAAACAGAC AAACCCATCT ACAAGCCAGG ACAAACAGTG
    AGATTCCGTG TTGTCTCTGT GGATGTCAGT TTTCATCCTT TGAATGAAAC CTTGCCGGTG
    GTTTATATTG AGAATCCCAA AAGGAACCGA ATTTTCCAAT GGCAAAATCT CAGATTGCAA
    GGAGGACTCA GCCAGCTCTC CTTCCCACTC TCAGTGGAGC CCACCCTGGG TGCTTATAAG
    GTCAAGCTGG AGAAGGAGTC AGGGGAAAAA ATAGAGCACC CCTTTGAAGT GAAGGAATAC
    GTTTTGCCCA AATTTGAGGT CCAAGTGAAA ATGCCCAAGA CGATTAGTTT TCTGGATGAA
    GAATTTGTAG TATCCGCCTG TGGCCTATAC ACGTATGGAA AGCCTGTCTC TGGTGTGGTG
    ACAATTAACA TATGCAGAAA ATACTCACAA TATCATTTTG CCTGCCATGG AATGCATTCG
    CAAGGTGTCT GTGAAGAATT CAGTCAACAG GCAGATGATA AAGGCTGTTT CACACAACTC
    GTGAAGACCA AAGTATTTCA GCTGAGACAA AGGGGGTATG ACATGACAAT ACAAGTGGAA
    GCCAAAATCA AAGAGGAGGG AACAGAGGTG GAACTAACTG GGCATGGATC ATGTGAAATC
    ACAAATATCC TGAGCAAGCT GAAATTTACG AAAGCGGATA CATATTATAG ACCTGGGCTT
    CCTTTTTATG GGCAGGTTCT TCTAGTTGAC GAGAAGGATC AGCCAATGGC CCATAAAACT
    GTAGTCGTCA GTGTGGACGT AACCACATAC CAATCCAGCC TTACCACGAA TGAACATGGT
    TTGGTGAACA TTTACATTGA TACCACCAAC TTCACATCTT CTCTCTTTAC AGTTATGGTG
    ACTTACAAAC AGAATGTCCA CTGTTTTGAT AATTGGTGGC TTGAAGAACT TCATAACCAA
    GCACAGCACA CTGTAAAGCA TATTTTCTCC CCGAGCAAGA GTTATGTTCA CCTTGAACTT
    GTTGCTGGTA CCATAACCTG TGGACAAACC CAGGAGATCC GGGCGCACTA CATCCTGAAT
    GGACAGATTC TGAAGGATGA AAAAGAATTA ACCTTCTATT ATTTGATCAA AGCAAGAGGA
    ATGATCTCCC GATCAGGAAT CCATGTGCTG TCCATTGAAC AAGGAGACTT GAAAGGGGAA
    TTTTCCTTCT CCTTTCGAGT GGAATCAGAC ATTGCTCCTG CAGCCCAGCT GCTCATTTAT
    GCTATTTTGC CCAATGGAGA AATTGTTGCC GATACTGAGA AACTCGAGAT TGAAAACTGT
    TTTGCCAATA AGGTAAATTT GAGCTTCTCC TCAAACCAGA GCCTGCCGGC CTCACACACC
    AACCTAGAGG TCAGGGCCTT TCCTAACTCC CTCTGTGCCC TCCGAGCAGT AGACCAGAGT
    GTTCTGCTGA TGAAGCCTGA AACTGAGCTC TCCCCTCAGT CGGTCTATAA TTTGCTACCA
    GTAAAGGGTA TCTTTAGTGT TGGTTATGGA GATCTCAGAA GAGAAGATAG TGAGCAATGT
    ATCCACGCAG AAGACTTTAT TCACGAGGGG ATCATTTACA CGCCAAAGCA GATCCTGGAT
    AATGATGACA TTTACAGTAT TTTTGAGTCC GCAGGATTAT ATATTTTTAC CAACTCAAAA
    ATCCACAAGC CGAATTTTTG TCAGCTGCCA CAGCCATATC CGGGAGTGCC TATGGCCCAT
    GCAGCAGTTA ACGTCCTGCC TCTAGCACGT GGTAGTGCAG GAGCAGTTTA TCCAATGGTC
    ATGAGACACA GTTCTCCAGC CGTGGAAGTG AAAGAGACAG TGCGGAAGTA CTTCCCTGAG
    ACCTGGATCT GGGACTTGGT ACCAGTCGAT TTATCAGGTA GAAGTGAGCT GGCAGTGAAG
    GTCCCTGACA CCATCACAGA GTGGAAGGCC AGTGCCCTCT GCTTGTCAGA AATGACAGGG
    CTAGGCCTCT CCCCCACCAT CTCTCTTCAA GTGTTCCAGC CTTTTTTCCT GGAACTCACT
    CTTCCCTACT CTGTGGTGCG AGGAGAAGCC TTCACACTCA AAGCAACCGT GTTCAACTAT
    TTGTCCCACT GCATTCGGGT CAGTGTGCAG CTGGAAGCCT CTCCTGACAA GTCGGCTGAC
    CTAGAGAGAA AGAACGAAGA AACTCCCTGT ATCTGTGGAA ATCACCAGAA AACTATGTTC
    TGGGCTGTGA CCCCAAAGTC ACTGGGAAAG ATGAATTTCA CAGCTACTGT GGAAGCCTTG
    CAGTCTCAGG AATTGTGTGG CAATGAGGTG CCAAGAGTCC CAGAACTTGG ACAGAAGGAT
    ACTGTAGTCA AGCCCTTATT AGTTGAGCCT GAAGGAATAG AAAAGGAGGA AACTTTCAAC
    ACACTCCTCT GTGCAGCAGA AACTGATGTA CCTGAAAAGT TGTCACTAAA AGTTCCTTCA
    GATGTGGTTG AAGGATCTGC CAGGGCGACA TATTCGGTTT TGGGTGACAT TCTAGGCTCT
    GCAATGCAAA ATCTTCAGAA TCTTCTCCGG ATGCCATATG GTTGTGGAGA GCAAAATATG
    GTCCTATTTG TCCCTAACAT CTATGTTCTG AACTACCTGA ATGAGACACG ACAACTGACA
    GAGACTATCA AGTCCAAAGC CATCAACTAC CTCATCAGTG GGTACCAAAG GCAGTTGAAT
    TATAAGCACA GTGATGGTTC ATACAGCACC TTTGGGGATC ATGATGGAAG AAGTCAGGGA
    AACACTTGGC TCACTGCATT TGTGCTCAAG TCTTTTGCTC AAGCCCGGTC ACACATTTTT
    GTGGAGATGT CACACATCAC GAGTTCCCTC ACCTGGCTCT CACAAAGACA GAAGGAAAAT
    GGTTGTTTCC AACGCTCTGG ATCACTACTA AACAATGCTG TTAAGGGTGG GGTAGATGAT
    GAATTGACGC TCTCTGCCTA CATCACCATT GCTCTGCTGG AGATGCCACT GCCTGTCACT
    CACCCTCTTG TCCGCAATGC TCTATTCTGC CTGGAAAGTG CCTGGGGATC CATCTCAGAG
    GCCCAAGGAA GTCTTGTCTA TACGAAGGCA TTATTGGCTT ATGCCTTTGC CCTAGCAGGA
    AACCAGGTCA AGCGAAATGA GCTTCTTAAA TCACTAGACA AAGATGCTGT GAAAGAAGAG
    GACTCAATCC ACTGGCAGCG TCCTGGGAAA GTTGAAGAAG TTACGACATT CTATCAACCC
    CGGGCTCCTT CAGTTGAAGT GGAGATGACC TCTTACCTGC TCCTTGCCTA TCTTACTGCT
    TCGCCTGCCC CATCTTCAGA AGACCTGTCT GTGGCTTCAC GTATTGTGAA ATGGATCACC
    AAGCAACAAA ACGCCAATGG GGGCTTCTCT TCCACTCAGG ATACAGTGGT AGCTCTCCAA
    GCCCTGTCCA AATATGGAGC AGCAACTTTC AGTAAAAGGG AGAAAACAAC TGTAGTCACT
    GTCAAGTCTT CAGAGACATT CTCCCAAAAA TTCGAAGTAC ACGATGCCAA TCGCCTGTTG
    CTACAAGAAG TCAGGTTGCC AGAGATTCCA GGGGAGTACA GCACAACAGT GTCAGGATCA
    GGATGTGTGT ACCTCCAGAC ATCCCTGAGA TACAACATCC TGCCCAAGAA AGAAGGCAAA
    GTTCCCTTCA ATCTGAAGGT TGATACTTTC CCCAAGAATT GTGATGGAGT AGACACTCAC
    AGGAAATTCC AGATTCACAT CAACATTAGT TATACTGGGG AACGATCAAG CTCCAACATG
    GTCATTGTTG ACGTGAAGAT GGTATCAGGC TTCATACCCG TGAAATCATC AGTGAGAAAG
    CTCCAAGAAA AGCCTCAGAT TCAAAGGACT GAAGTGAGCA CCAACCATGT TCTGATTTAC
    TTTGAAGAGC TAACCAATCA AACTCTGAGT TTCTCCTTCT CAGTGGAACA AGACATCCAA
    GTTGACAATT TAAAACCAGC TACTGTAAAA GCCTATGACT ATTATGAGAC AGAGGAATTT
    ACTATTGAAG AGTACAGGGA TCCCTGCAGT GCTGAATCTG GACACAGAAC TGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.