SLIT2 cDNA ORF clone, Canis lupus familiaris(dog)

The following SLIT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLIT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe287416 XM_022417277.1
Latest version!
Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe287415 XM_022417276.1
Latest version!
Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe35885 XM_014112671.2
Latest version!
Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe35886 XM_014112672.2
Latest version!
Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe35887 XM_014112673.2
Latest version!
Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe35888 XM_014112674.2
Latest version!
Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe35889 XM_014112675.2
Latest version!
Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
View Old Accession Versions >>
OCe35889 XM_014112675.1 Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe35887 XM_014112673.1 Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe35886 XM_014112672.1 Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe35885 XM_014112671.1 Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe35888 XM_014112674.1 Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe287416
    Clone ID Related Accession (Same CDS sequence) XM_022417277.1
    Accession Version XM_022417277.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4242bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product slit homolog 2 protein isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTCTGT TTTCATCAGC TCTGCACGCG TGTGATCTCA GTGAAAACCA AATTCAGGCA 
    ATTCCAAGGA AGGCTTTCCG TGGGGCAGTT GACATTAAAA ATTTGCAACT GGATTACAAC
    CAGATCAGCT GTATTGAAGA TGGGGCATTT AGAGCTCTGC GGGACCTGGA AGTGCTCACT
    CTCAACAATA ACAACATTAC TAGACTTTCT GTGGCAAGTT TCAACCATAT GCCTAAACTT
    AGGACTTTTC GGCTGCATTC AAACAATCTG TATTGCGACT GCCACCTGGC CTGGCTTTCT
    GACTGGCTGC GCCAAAGGCC CCGGGTTGGT CTCTACACTC AGTGTATGGG CCCATCCCAC
    CTGAGGGGTC ATAACGTAGC CGAGGTTCAA AAACGCGAAT TTGTCTGCAG TGATGAGGAA
    GAAGGCCACC AGTCCTTCAT GGCTCCTTCT TGCAGCGTCC TGCATTGTCC AGCCGCTTGT
    ACCTGTAGCA ACAATATCGT AGACTGTCGT GGGAAAGGTC TCACTGAGAT CCCCACGAAC
    CTGCCAGAGA CCATCACAGA AATACGTTTG GAACAGAACT CAATCAAGGT CATCCCTCCT
    GGAGCTTTCT CACCATATAA AAAGCTTAGA AGAATTGACC TGAGCAATAA TCAGATCTCT
    GAACTAGCAC CGGACGCTTT CCAAGGACTA CGCTCTCTGA ATTCACTTGT CCTCTATGGA
    AATAAAATCA CGGAACTCCC AAAAAGTTTA TTTGAAGGAC TGTTTTCCTT ACAGCTGCTA
    TTATTGAATG CCAACAAGAT AAACTGCCTT CGGGTAGATG CTTTTCAGGA TCTGCACAAC
    CTGAATCTTC TCTCCCTGTA CGACAACAAG CTGCAGACCA TCGCCAAGGG GACCTTCTCA
    CCTCTCCGGG CCATTCAGAC CATGCACCTG GCCCAGAACC CCTTTATTTG TGACTGCCAT
    CTCAAGTGGC TGGCGGACTA TCTCCACACC AACCCCATCG AGACCAGTGG TGCCCGGTGC
    ACCAGCCCCC GGCGCCTGGC AAACAAAAGA ATCGGACAGA TCAAAAGCAA GAAATTCCGT
    TGTTCAGCTA AAGAACAGTA TTTCATTCCA GGTACAGAAG ATTATCGATC AAAATTAAGC
    GGGGACTGCT TTGCAGATCT GGCTTGCCCT GAAAAGTGCC GCTGTGAAGG AACCACAGTA
    GATTGCTCCA ATCAAAAACT CACCAAAATC CCAGACCACA TCCCCCAGTA CACTGCAGAG
    CTGCGTCTCA ATAATAATGA ATTCACAGTG CTGGAAGCTA CAGGAATCTT CAAGAAACTT
    CCGCAGTTAC GTAAAATAAA CTTCAGCAAC AACAAGATCA CAGACATTGA AGAAGGAGCA
    TTTGAAGGAG CAGCTGGTGT AAACGAAATC CTTCTCACGA GTAACCGTTT GGAAAATGTT
    CAGCATAAGA TGTTCAAGGG ATTGGAAAGC CTGAAAACGT TGATGTTGCG AAGCAATCGC
    ATAAGCTGCG TTGGCAACGA TAGCTTCATA GGCCTGAGCT CTGTGCGGTT GCTTTCGCTG
    TACGATAATC AGATCGCCAC CATCGCGCCG GGGGCGTTCG ACACCCTGCA CTCGTTGTCC
    ACCCTAAACC TGTTGGCCAA CCCTTTTAAC TGCAACTGCT ACCTGGCTTG GCTGGGCGAG
    TGGCTCAGGA AGAAAAGAAT TGTAACCGGA AATCCTCGCT GTCAAAAACC ATACTTCCTC
    AAAGAAATCC CCATCCAGGA CGTCGCCATT CAAGACTTCA CGTGTGACGA CGGAAATGAC
    GACAGTAGCT GTTCTCCACT CTCGCGCTGT CCCACGGAAT GCACGTGCTT GGATACAGTT
    GTCCGATGTA GCAACAAGGG CCTGAAGGTC TTGCCCAAAG GTATTCCCAG AGACGTCACT
    GAACTGTATC TGGATGGGAA CCACTTTACC TTGGTTCCCA AGGAGCTCTA TAACTACAAA
    CATCTAACGC TTATAGACCT GAGCAACAAC CGCATAAGCA CTCTTTCTAA TCAGAGCTTC
    AGCAACATGA CCCAGCTCCT CACCCTAATT CTCAGTTACA ACCGTTTGAG ATGTATTCCT
    CCTCGAACCT TCGATGGACT CAAGTCTCTC CGATTACTTT CATTACATGG AAATGACATT
    TCTGTTGTGC CTGAAGGTGC TTTCAGTGAT CTCTCTGCAT TATCACACCT AGCAATCGGA
    GCCAACCCCC TTTACTGTGA TTGCAACATG CAGTGGTTAT CGGACTGGGT AAAGTCGGAA
    TACAAAGAAC CCGGGATTGC TCGCTGTGCC GGCCCCGGAG AAATGGCAGA TAAATTATTA
    CTCACGACTC CCTCCAAAAA ATTTACATGT CAAGGTCCTG TGGATATCAA TATTCTAGCT
    AAATGTAATC CCTGCTTATC AAACCCATGT AAGAATGATG GCACCTGTAA CAATGATCCA
    GTCGACTTTT ATCGCTGTAC CTGTCCGTAT GGTTTCAAGG GGCAGGACTG TGATGTCCCA
    ATCCACGCAT GCATCAGTAA CCCGTGTACA CATGGAGGAA CTTGCCACTT AAAGGAGGGA
    GAAAAAGATG GATTCTGGTG TATTTGTGCC GATGGATTTG AAGGAGAAAA TTGTGAAGTC
    AATGTTGATG ACTGTGAAGA TAATGACTGT GAAAATAACT CTACGTGTGT CGATGGAATT
    AATAACTACA CATGCCTTTG TCCGCCTGAG TACACAGGCG AGTTGTGTGA GGAGAAGCTG
    GACTTCTGCG CTCAGGACCT GAACCCCTGC CAGCACGACT CCAAGTGCAT CCTGATGCCC
    AAAGGATTCA AATGCGACTG CACGCCGGGG TACGTGGGCG AGCACTGCGA CATCGACTTC
    GACGACTGCC AGGATCACAA GTGTAAAAAC GGAGCGCACT GCACGGACGC GGTGAACGGC
    TACACGTGCA CCTGCCCCGA AGGCTACAGC GGCTTGTTCT GTGAATTCTC CCCGCCCATG
    GTCCTCCCAC GCACCAGCCC CTGTGACAAC TTCGACTGTC AGAACGGGGC GCAGTGCATC
    GTCAGGGCGG GCGAGCCAAT CTGCCAGTGT CTGCCCGGCT ACCAGGGGGA CAAGTGTGAG
    AAGTTGGTCA GCGTGAACTT CGTGAACAAA GAGTCGTATC TTCAAATTCC TTCAGCCAAG
    GTCCGGCCCC AAACGAACAT CACCCTGCAG ATTGCCACCG ACGAAGACAG CGGGATCCTC
    CTGTACAAGG GCGACAAGGA CCACATTGCC GTGGAGCTGT ATCGGGGACG GGTGCGCGCC
    AGCTACGACA CCGGCTCGCA CCCCGCTTCT GCCATTTACA GCGTGGAGAC GATCAATGAT
    GGAAACTTTC ACATTGTGGA ACTACTTGCC CTGGATCAGA GCCTGTCCCT CTCCGTGGAT
    GGAGGGAGCC CCAAAATCAT CACCAACTTG TCAAAGCAGT CCACTCTGAA TTTTGACTCT
    CCACTCTATG TAGGAGGCAT GCCCGGGAGG AACAACGTGG CCGCGGCCCT GCGCCAGGCC
    CCGGGGCACA ACGGCACCAG CTTCCACGGC TGCATCCGGA ACCTGTATAT CAACAGCGAG
    CTCCAGGACT TCCGCCAGGT GCCCATGCAG ACCGGCATCC TGCCCGGCTG CGAGCCGTGC
    CACAGGAAGG TGTGTGCCCA CGGCGCGTGC CAGCCCAGCA GCCAGTCGGG CTTCACCTGC
    GAGTGCGAGG AGGGCTGGAC GGGGCCCCTG TGTGACCAGA GGACCAACGA CCCCTGTCTC
    GGGAACAAAT GTGTGCACGG CACCTGCTTG CCCATCAACG CCTTCTCCTA CAGCTGTAAG
    TGTCTGGAGG GCCACGGGGG CGTCCTCTGC GACGAAGAGG AGGACCTGTT CAACCCCTGC
    CAGGCCATCA GGTGCAAGCA CGGGAAATGC AGGCTCTCGG GCCTGGGCCA GCCCTACTGC
    GAATGCAGCA GCGGGTACAC GGGGGATAGC TGCGACCGAG AAGTGTCCTG TCGGGGCGAG
    CGCGTCCGGG ACTACTACCC AAAGCAGCAG GGCTACGCGG CCTGCCAGAC CACCAAGAAG
    GTGTCGCGGC TGGAGTGCAG GGGCGGCTGC GCGGCCGGGC AGTGCTGCGG GCCGCTGCGG
    AGCAAGCGGC GGAAATACTC CTTCGAGTGC ACGGACGGCT CGTCGTTCGT GGACGAGGTG
    GAGAAGGTGG TCAAGTGCGG CTGCAGCAGG TGCGCCGCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272985.1
    CDS16..4257
    Translation

    Target ORF information:

    RefSeq Version XM_022417277.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417277.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTCTGT TTTCATCAGC TCTGCACGCG TGTGATCTCA GTGAAAACCA AATTCAGGCA 
    ATTCCAAGGA AGGCTTTCCG TGGGGCAGTT GACATTAAAA ATTTGCAACT GGATTACAAC
    CAGATCAGCT GTATTGAAGA TGGGGCATTT AGAGCTCTGC GGGACCTGGA AGTGCTCACT
    CTCAACAATA ACAACATTAC TAGACTTTCT GTGGCAAGTT TCAACCATAT GCCTAAACTT
    AGGACTTTTC GGCTGCATTC AAACAATCTG TATTGCGACT GCCACCTGGC CTGGCTTTCT
    GACTGGCTGC GCCAAAGGCC CCGGGTTGGT CTCTACACTC AGTGTATGGG CCCATCCCAC
    CTGAGGGGTC ATAACGTAGC CGAGGTTCAA AAACGCGAAT TTGTCTGCAG TGATGAGGAA
    GAAGGCCACC AGTCCTTCAT GGCTCCTTCT TGCAGCGTCC TGCATTGTCC AGCCGCTTGT
    ACCTGTAGCA ACAATATCGT AGACTGTCGT GGGAAAGGTC TCACTGAGAT CCCCACGAAC
    CTGCCAGAGA CCATCACAGA AATACGTTTG GAACAGAACT CAATCAAGGT CATCCCTCCT
    GGAGCTTTCT CACCATATAA AAAGCTTAGA AGAATTGACC TGAGCAATAA TCAGATCTCT
    GAACTAGCAC CGGACGCTTT CCAAGGACTA CGCTCTCTGA ATTCACTTGT CCTCTATGGA
    AATAAAATCA CGGAACTCCC AAAAAGTTTA TTTGAAGGAC TGTTTTCCTT ACAGCTGCTA
    TTATTGAATG CCAACAAGAT AAACTGCCTT CGGGTAGATG CTTTTCAGGA TCTGCACAAC
    CTGAATCTTC TCTCCCTGTA CGACAACAAG CTGCAGACCA TCGCCAAGGG GACCTTCTCA
    CCTCTCCGGG CCATTCAGAC CATGCACCTG GCCCAGAACC CCTTTATTTG TGACTGCCAT
    CTCAAGTGGC TGGCGGACTA TCTCCACACC AACCCCATCG AGACCAGTGG TGCCCGGTGC
    ACCAGCCCCC GGCGCCTGGC AAACAAAAGA ATCGGACAGA TCAAAAGCAA GAAATTCCGT
    TGTTCAGCTA AAGAACAGTA TTTCATTCCA GGTACAGAAG ATTATCGATC AAAATTAAGC
    GGGGACTGCT TTGCAGATCT GGCTTGCCCT GAAAAGTGCC GCTGTGAAGG AACCACAGTA
    GATTGCTCCA ATCAAAAACT CACCAAAATC CCAGACCACA TCCCCCAGTA CACTGCAGAG
    CTGCGTCTCA ATAATAATGA ATTCACAGTG CTGGAAGCTA CAGGAATCTT CAAGAAACTT
    CCGCAGTTAC GTAAAATAAA CTTCAGCAAC AACAAGATCA CAGACATTGA AGAAGGAGCA
    TTTGAAGGAG CAGCTGGTGT AAACGAAATC CTTCTCACGA GTAACCGTTT GGAAAATGTT
    CAGCATAAGA TGTTCAAGGG ATTGGAAAGC CTGAAAACGT TGATGTTGCG AAGCAATCGC
    ATAAGCTGCG TTGGCAACGA TAGCTTCATA GGCCTGAGCT CTGTGCGGTT GCTTTCGCTG
    TACGATAATC AGATCGCCAC CATCGCGCCG GGGGCGTTCG ACACCCTGCA CTCGTTGTCC
    ACCCTAAACC TGTTGGCCAA CCCTTTTAAC TGCAACTGCT ACCTGGCTTG GCTGGGCGAG
    TGGCTCAGGA AGAAAAGAAT TGTAACCGGA AATCCTCGCT GTCAAAAACC ATACTTCCTC
    AAAGAAATCC CCATCCAGGA CGTCGCCATT CAAGACTTCA CGTGTGACGA CGGAAATGAC
    GACAGTAGCT GTTCTCCACT CTCGCGCTGT CCCACGGAAT GCACGTGCTT GGATACAGTT
    GTCCGATGTA GCAACAAGGG CCTGAAGGTC TTGCCCAAAG GTATTCCCAG AGACGTCACT
    GAACTGTATC TGGATGGGAA CCACTTTACC TTGGTTCCCA AGGAGCTCTA TAACTACAAA
    CATCTAACGC TTATAGACCT GAGCAACAAC CGCATAAGCA CTCTTTCTAA TCAGAGCTTC
    AGCAACATGA CCCAGCTCCT CACCCTAATT CTCAGTTACA ACCGTTTGAG ATGTATTCCT
    CCTCGAACCT TCGATGGACT CAAGTCTCTC CGATTACTTT CATTACATGG AAATGACATT
    TCTGTTGTGC CTGAAGGTGC TTTCAGTGAT CTCTCTGCAT TATCACACCT AGCAATCGGA
    GCCAACCCCC TTTACTGTGA TTGCAACATG CAGTGGTTAT CGGACTGGGT AAAGTCGGAA
    TACAAAGAAC CCGGGATTGC TCGCTGTGCC GGCCCCGGAG AAATGGCAGA TAAATTATTA
    CTCACGACTC CCTCCAAAAA ATTTACATGT CAAGGTCCTG TGGATATCAA TATTCTAGCT
    AAATGTAATC CCTGCTTATC AAACCCATGT AAGAATGATG GCACCTGTAA CAATGATCCA
    GTCGACTTTT ATCGCTGTAC CTGTCCGTAT GGTTTCAAGG GGCAGGACTG TGATGTCCCA
    ATCCACGCAT GCATCAGTAA CCCGTGTACA CATGGAGGAA CTTGCCACTT AAAGGAGGGA
    GAAAAAGATG GATTCTGGTG TATTTGTGCC GATGGATTTG AAGGAGAAAA TTGTGAAGTC
    AATGTTGATG ACTGTGAAGA TAATGACTGT GAAAATAACT CTACGTGTGT CGATGGAATT
    AATAACTACA CATGCCTTTG TCCGCCTGAG TACACAGGCG AGTTGTGTGA GGAGAAGCTG
    GACTTCTGCG CTCAGGACCT GAACCCCTGC CAGCACGACT CCAAGTGCAT CCTGATGCCC
    AAAGGATTCA AATGCGACTG CACGCCGGGG TACGTGGGCG AGCACTGCGA CATCGACTTC
    GACGACTGCC AGGATCACAA GTGTAAAAAC GGAGCGCACT GCACGGACGC GGTGAACGGC
    TACACGTGCA CCTGCCCCGA AGGCTACAGC GGCTTGTTCT GTGAATTCTC CCCGCCCATG
    GTCCTCCCAC GCACCAGCCC CTGTGACAAC TTCGACTGTC AGAACGGGGC GCAGTGCATC
    GTCAGGGCGG GCGAGCCAAT CTGCCAGTGT CTGCCCGGCT ACCAGGGGGA CAAGTGTGAG
    AAGTTGGTCA GCGTGAACTT CGTGAACAAA GAGTCGTATC TTCAAATTCC TTCAGCCAAG
    GTCCGGCCCC AAACGAACAT CACCCTGCAG ATTGCCACCG ACGAAGACAG CGGGATCCTC
    CTGTACAAGG GCGACAAGGA CCACATTGCC GTGGAGCTGT ATCGGGGACG GGTGCGCGCC
    AGCTACGACA CCGGCTCGCA CCCCGCTTCT GCCATTTACA GCGTGGAGAC GATCAATGAT
    GGAAACTTTC ACATTGTGGA ACTACTTGCC CTGGATCAGA GCCTGTCCCT CTCCGTGGAT
    GGAGGGAGCC CCAAAATCAT CACCAACTTG TCAAAGCAGT CCACTCTGAA TTTTGACTCT
    CCACTCTATG TAGGAGGCAT GCCCGGGAGG AACAACGTGG CCGCGGCCCT GCGCCAGGCC
    CCGGGGCACA ACGGCACCAG CTTCCACGGC TGCATCCGGA ACCTGTATAT CAACAGCGAG
    CTCCAGGACT TCCGCCAGGT GCCCATGCAG ACCGGCATCC TGCCCGGCTG CGAGCCGTGC
    CACAGGAAGG TGTGTGCCCA CGGCGCGTGC CAGCCCAGCA GCCAGTCGGG CTTCACCTGC
    GAGTGCGAGG AGGGCTGGAC GGGGCCCCTG TGTGACCAGA GGACCAACGA CCCCTGTCTC
    GGGAACAAAT GTGTGCACGG CACCTGCTTG CCCATCAACG CCTTCTCCTA CAGCTGTAAG
    TGTCTGGAGG GCCACGGGGG CGTCCTCTGC GACGAAGAGG AGGACCTGTT CAACCCCTGC
    CAGGCCATCA GGTGCAAGCA CGGGAAATGC AGGCTCTCGG GCCTGGGCCA GCCCTACTGC
    GAATGCAGCA GCGGGTACAC GGGGGATAGC TGCGACCGAG AAGTGTCCTG TCGGGGCGAG
    CGCGTCCGGG ACTACTACCC AAAGCAGCAG GGCTACGCGG CCTGCCAGAC CACCAAGAAG
    GTGTCGCGGC TGGAGTGCAG GGGCGGCTGC GCGGCCGGGC AGTGCTGCGG GCCGCTGCGG
    AGCAAGCGGC GGAAATACTC CTTCGAGTGC ACGGACGGCT CGTCGTTCGT GGACGAGGTG
    GAGAAGGTGG TCAAGTGCGG CTGCAGCAGG TGCGCCGCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287415
    Clone ID Related Accession (Same CDS sequence) XM_022417276.1
    Accession Version XM_022417276.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product slit homolog 2 protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
    GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC
    TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG
    GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC
    CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG
    GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG
    TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG
    GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC
    AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC
    ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT
    TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG
    GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT
    TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG
    AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT
    CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC
    TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT
    GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC
    AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC
    TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC
    CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG
    TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA
    AGCGGGGACT GCTTTGCAGA TCTGGCTTGC CCTGAAAAGT GCCGCTGTGA AGGAACCACA
    GTAGATTGCT CCAATCAAAA ACTCACCAAA ATCCCAGACC ACATCCCCCA GTACACTGCA
    GAGCTGCGTC TCAATAATAA TGAATTCACA GTGCTGGAAG CTACAGGAAT CTTCAAGAAA
    CTTCCGCAGT TACGTAAAAT AAACTTCAGC AACAACAAGA TCACAGACAT TGAAGAAGGA
    GCATTTGAAG GAGCAGCTGG TGTAAACGAA ATCCTTCTCA CGAGTAACCG TTTGGAAAAT
    GTTCAGCATA AGATGTTCAA GGGATTGGAA AGCCTGAAAA CGTTGATGTT GCGAAGCAAT
    CGCATAAGCT GCGTTGGCAA CGATAGCTTC ATAGGCCTGA GCTCTGTGCG GTTGCTTTCG
    CTGTACGATA ATCAGATCGC CACCATCGCG CCGGGGGCGT TCGACACCCT GCACTCGTTG
    TCCACCCTAA ACCTGTTGGC CAACCCTTTT AACTGCAACT GCTACCTGGC TTGGCTGGGC
    GAGTGGCTCA GGAAGAAAAG AATTGTAACC GGAAATCCTC GCTGTCAAAA ACCATACTTC
    CTCAAAGAAA TCCCCATCCA GGACGTCGCC ATTCAAGACT TCACGTGTGA CGACGGAAAT
    GACGACAGTA GCTGTTCTCC ACTCTCGCGC TGTCCCACGG AATGCACGTG CTTGGATACA
    GTTGTCCGAT GTAGCAACAA GGGCCTGAAG GTCTTGCCCA AAGGTATTCC CAGAGACGTC
    ACTGAACTGT ATCTGGATGG GAACCACTTT ACCTTGGTTC CCAAGGAGCT CTATAACTAC
    AAACATCTAA CGCTTATAGA CCTGAGCAAC AACCGCATAA GCACTCTTTC TAATCAGAGC
    TTCAGCAACA TGACCCAGCT CCTCACCCTA ATTCTCAGTT ACAACCGTTT GAGATGTATT
    CCTCCTCGAA CCTTCGATGG ACTCAAGTCT CTCCGATTAC TTTCATTACA TGGAAATGAC
    ATTTCTGTTG TGCCTGAAGG TGCTTTCAGT GATCTCTCTG CATTATCACA CCTAGCAATC
    GGAGCCAACC CCCTTTACTG TGATTGCAAC ATGCAGTGGT TATCGGACTG GGTAAAGTCG
    GAATACAAAG AACCCGGGAT TGCTCGCTGT GCCGGCCCCG GAGAAATGGC AGATAAATTA
    TTACTCACGA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CTGTGGATAT CAATATTCTA
    GCTAAATGTA ATCCCTGCTT ATCAAACCCA TGTAAGAATG ATGGCACCTG TAACAATGAT
    CCAGTCGACT TTTATCGCTG TACCTGTCCG TATGGTTTCA AGGGGCAGGA CTGTGATGTC
    CCAATCCACG CATGCATCAG TAACCCGTGT ACACATGGAG GAACTTGCCA CTTAAAGGAG
    GGAGAAAAAG ATGGATTCTG GTGTATTTGT GCCGATGGAT TTGAAGGAGA AAATTGTGAA
    GTCAATGTTG ATGACTGTGA AGATAATGAC TGTGAAAATA ACTCTACGTG TGTCGATGGA
    ATTAATAACT ACACATGCCT TTGTCCGCCT GAGTACACAG GCGAGTTGTG TGAGGAGAAG
    CTGGACTTCT GCGCTCAGGA CCTGAACCCC TGCCAGCACG ACTCCAAGTG CATCCTGATG
    CCCAAAGGAT TCAAATGCGA CTGCACGCCG GGGTACGTGG GCGAGCACTG CGACATCGAC
    TTCGACGACT GCCAGGATCA CAAGTGTAAA AACGGAGCGC ACTGCACGGA CGCGGTGAAC
    GGCTACACGT GCACCTGCCC CGAAGGCTAC AGCGGCTTGT TCTGTGAATT CTCCCCGCCC
    ATGGTCCTCC CACGCACCAG CCCCTGTGAC AACTTCGACT GTCAGAACGG GGCGCAGTGC
    ATCGTCAGGG CGGGCGAGCC AATCTGCCAG TGTCTGCCCG GCTACCAGGG GGACAAGTGT
    GAGAAGTTGG TCAGCGTGAA CTTCGTGAAC AAAGAGTCGT ATCTTCAAAT TCCTTCAGCC
    AAGGTCCGGC CCCAAACGAA CATCACCCTG CAGATTGCCA CCGACGAAGA CAGCGGGATC
    CTCCTGTACA AGGGCGACAA GGACCACATT GCCGTGGAGC TGTATCGGGG ACGGGTGCGC
    GCCAGCTACG ACACCGGCTC GCACCCCGCT TCTGCCATTT ACAGCGTGGA GACGATCAAT
    GATGGAAACT TTCACATTGT GGAACTACTT GCCCTGGATC AGAGCCTGTC CCTCTCCGTG
    GATGGAGGGA GCCCCAAAAT CATCACCAAC TTGTCAAAGC AGTCCACTCT GAATTTTGAC
    TCTCCACTCT ATGTAGGAGG CATGCCCGGG AGGAACAACG TGGCCGCGGC CCTGCGCCAG
    GCCCCGGGGC ACAACGGCAC CAGCTTCCAC GGCTGCATCC GGAACCTGTA TATCAACAGC
    GAGCTCCAGG ACTTCCGCCA GGTGCCCATG CAGACCGGCA TCCTGCCCGG CTGCGAGCCG
    TGCCACAGGA AGGTGTGTGC CCACGGCGCG TGCCAGCCCA GCAGCCAGTC GGGCTTCACC
    TGCGAGTGCG AGGAGGGCTG GACGGGGCCC CTGTGTGACC AGAGGACCAA CGACCCCTGT
    CTCGGGAACA AATGTGTGCA CGGCACCTGC TTGCCCATCA ACGCCTTCTC CTACAGCTGT
    AAGTGTCTGG AGGGCCACGG GGGCGTCCTC TGCGACGAAG AGGAGGACCT GTTCAACCCC
    TGCCAGGCCA TCAGGTGCAA GCACGGGAAA TGCAGGCTCT CGGGCCTGGG CCAGCCCTAC
    TGCGAATGCA GCAGCGGGTA CACGGGGGAT AGCTGCGACC GAGTGTCCTG TCGGGGCGAG
    CGCGTCCGGG ACTACTACCC AAAGCAGCAG GGCTACGCGG CCTGCCAGAC CACCAAGAAG
    GTGTCGCGGC TGGAGTGCAG GGGCGGCTGC GCGGCCGGGC AGTGCTGCGG GCCGCTGCGG
    AGCAAGCGGC GGAAATACTC CTTCGAGTGC ACGGACGGCT CGTCGTTCGT GGACGAGGTG
    GAGAAGGTGG TCAAGTGCGG CTGCAGCAGG TGCGCCGCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272984.1
    CDS213..4814
    Translation

    Target ORF information:

    RefSeq Version XM_022417276.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417276.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
    GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC
    TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG
    GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC
    CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG
    GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG
    TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG
    GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC
    AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC
    ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT
    TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG
    GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT
    TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG
    AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT
    CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC
    TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT
    GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC
    AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC
    TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC
    CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG
    TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA
    AGCGGGGACT GCTTTGCAGA TCTGGCTTGC CCTGAAAAGT GCCGCTGTGA AGGAACCACA
    GTAGATTGCT CCAATCAAAA ACTCACCAAA ATCCCAGACC ACATCCCCCA GTACACTGCA
    GAGCTGCGTC TCAATAATAA TGAATTCACA GTGCTGGAAG CTACAGGAAT CTTCAAGAAA
    CTTCCGCAGT TACGTAAAAT AAACTTCAGC AACAACAAGA TCACAGACAT TGAAGAAGGA
    GCATTTGAAG GAGCAGCTGG TGTAAACGAA ATCCTTCTCA CGAGTAACCG TTTGGAAAAT
    GTTCAGCATA AGATGTTCAA GGGATTGGAA AGCCTGAAAA CGTTGATGTT GCGAAGCAAT
    CGCATAAGCT GCGTTGGCAA CGATAGCTTC ATAGGCCTGA GCTCTGTGCG GTTGCTTTCG
    CTGTACGATA ATCAGATCGC CACCATCGCG CCGGGGGCGT TCGACACCCT GCACTCGTTG
    TCCACCCTAA ACCTGTTGGC CAACCCTTTT AACTGCAACT GCTACCTGGC TTGGCTGGGC
    GAGTGGCTCA GGAAGAAAAG AATTGTAACC GGAAATCCTC GCTGTCAAAA ACCATACTTC
    CTCAAAGAAA TCCCCATCCA GGACGTCGCC ATTCAAGACT TCACGTGTGA CGACGGAAAT
    GACGACAGTA GCTGTTCTCC ACTCTCGCGC TGTCCCACGG AATGCACGTG CTTGGATACA
    GTTGTCCGAT GTAGCAACAA GGGCCTGAAG GTCTTGCCCA AAGGTATTCC CAGAGACGTC
    ACTGAACTGT ATCTGGATGG GAACCACTTT ACCTTGGTTC CCAAGGAGCT CTATAACTAC
    AAACATCTAA CGCTTATAGA CCTGAGCAAC AACCGCATAA GCACTCTTTC TAATCAGAGC
    TTCAGCAACA TGACCCAGCT CCTCACCCTA ATTCTCAGTT ACAACCGTTT GAGATGTATT
    CCTCCTCGAA CCTTCGATGG ACTCAAGTCT CTCCGATTAC TTTCATTACA TGGAAATGAC
    ATTTCTGTTG TGCCTGAAGG TGCTTTCAGT GATCTCTCTG CATTATCACA CCTAGCAATC
    GGAGCCAACC CCCTTTACTG TGATTGCAAC ATGCAGTGGT TATCGGACTG GGTAAAGTCG
    GAATACAAAG AACCCGGGAT TGCTCGCTGT GCCGGCCCCG GAGAAATGGC AGATAAATTA
    TTACTCACGA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CTGTGGATAT CAATATTCTA
    GCTAAATGTA ATCCCTGCTT ATCAAACCCA TGTAAGAATG ATGGCACCTG TAACAATGAT
    CCAGTCGACT TTTATCGCTG TACCTGTCCG TATGGTTTCA AGGGGCAGGA CTGTGATGTC
    CCAATCCACG CATGCATCAG TAACCCGTGT ACACATGGAG GAACTTGCCA CTTAAAGGAG
    GGAGAAAAAG ATGGATTCTG GTGTATTTGT GCCGATGGAT TTGAAGGAGA AAATTGTGAA
    GTCAATGTTG ATGACTGTGA AGATAATGAC TGTGAAAATA ACTCTACGTG TGTCGATGGA
    ATTAATAACT ACACATGCCT TTGTCCGCCT GAGTACACAG GCGAGTTGTG TGAGGAGAAG
    CTGGACTTCT GCGCTCAGGA CCTGAACCCC TGCCAGCACG ACTCCAAGTG CATCCTGATG
    CCCAAAGGAT TCAAATGCGA CTGCACGCCG GGGTACGTGG GCGAGCACTG CGACATCGAC
    TTCGACGACT GCCAGGATCA CAAGTGTAAA AACGGAGCGC ACTGCACGGA CGCGGTGAAC
    GGCTACACGT GCACCTGCCC CGAAGGCTAC AGCGGCTTGT TCTGTGAATT CTCCCCGCCC
    ATGGTCCTCC CACGCACCAG CCCCTGTGAC AACTTCGACT GTCAGAACGG GGCGCAGTGC
    ATCGTCAGGG CGGGCGAGCC AATCTGCCAG TGTCTGCCCG GCTACCAGGG GGACAAGTGT
    GAGAAGTTGG TCAGCGTGAA CTTCGTGAAC AAAGAGTCGT ATCTTCAAAT TCCTTCAGCC
    AAGGTCCGGC CCCAAACGAA CATCACCCTG CAGATTGCCA CCGACGAAGA CAGCGGGATC
    CTCCTGTACA AGGGCGACAA GGACCACATT GCCGTGGAGC TGTATCGGGG ACGGGTGCGC
    GCCAGCTACG ACACCGGCTC GCACCCCGCT TCTGCCATTT ACAGCGTGGA GACGATCAAT
    GATGGAAACT TTCACATTGT GGAACTACTT GCCCTGGATC AGAGCCTGTC CCTCTCCGTG
    GATGGAGGGA GCCCCAAAAT CATCACCAAC TTGTCAAAGC AGTCCACTCT GAATTTTGAC
    TCTCCACTCT ATGTAGGAGG CATGCCCGGG AGGAACAACG TGGCCGCGGC CCTGCGCCAG
    GCCCCGGGGC ACAACGGCAC CAGCTTCCAC GGCTGCATCC GGAACCTGTA TATCAACAGC
    GAGCTCCAGG ACTTCCGCCA GGTGCCCATG CAGACCGGCA TCCTGCCCGG CTGCGAGCCG
    TGCCACAGGA AGGTGTGTGC CCACGGCGCG TGCCAGCCCA GCAGCCAGTC GGGCTTCACC
    TGCGAGTGCG AGGAGGGCTG GACGGGGCCC CTGTGTGACC AGAGGACCAA CGACCCCTGT
    CTCGGGAACA AATGTGTGCA CGGCACCTGC TTGCCCATCA ACGCCTTCTC CTACAGCTGT
    AAGTGTCTGG AGGGCCACGG GGGCGTCCTC TGCGACGAAG AGGAGGACCT GTTCAACCCC
    TGCCAGGCCA TCAGGTGCAA GCACGGGAAA TGCAGGCTCT CGGGCCTGGG CCAGCCCTAC
    TGCGAATGCA GCAGCGGGTA CACGGGGGAT AGCTGCGACC GAGTGTCCTG TCGGGGCGAG
    CGCGTCCGGG ACTACTACCC AAAGCAGCAG GGCTACGCGG CCTGCCAGAC CACCAAGAAG
    GTGTCGCGGC TGGAGTGCAG GGGCGGCTGC GCGGCCGGGC AGTGCTGCGG GCCGCTGCGG
    AGCAAGCGGC GGAAATACTC CTTCGAGTGC ACGGACGGCT CGTCGTTCGT GGACGAGGTG
    GAGAAGGTGG TCAAGTGCGG CTGCAGCAGG TGCGCCGCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35885
    Clone ID Related Accession (Same CDS sequence) XM_014112671.1 , XM_014112671.2
    Accession Version XM_014112671.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product slit homolog 2 protein isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
    GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC
    TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG
    GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC
    CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG
    GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG
    TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG
    GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC
    AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC
    ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT
    TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG
    GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT
    TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG
    AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT
    CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC
    TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT
    GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC
    AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC
    TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC
    CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG
    TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA
    AGCGGGGACT GCTTTGCAGA TCTGGCTTGC CCTGAAAAGT GCCGCTGTGA AGGAACCACA
    GTAGATTGCT CCAATCAAAA ACTCACCAAA ATCCCAGACC ACATCCCCCA GTACACTGCA
    GAGCTGCGTC TCAATAATAA TGAATTCACA GTGCTGGAAG CTACAGGAAT CTTCAAGAAA
    CTTCCGCAGT TACGTAAAAT AAACTTCAGC AACAACAAGA TCACAGACAT TGAAGAAGGA
    GCATTTGAAG GAGCAGCTGG TGTAAACGAA ATCCTTCTCA CGAGTAACCG TTTGGAAAAT
    GTTCAGCATA AGATGTTCAA GGGATTGGAA AGCCTGAAAA CGTTGATGTT GCGAAGCAAT
    CGCATAAGCT GCGTTGGCAA CGATAGCTTC ATAGGCCTGA GCTCTGTGCG GTTGCTTTCG
    CTGTACGATA ATCAGATCGC CACCATCGCG CCGGGGGCGT TCGACACCCT GCACTCGTTG
    TCCACCCTAA ACCTGTTGGC CAACCCTTTT AACTGCAACT GCTACCTGGC TTGGCTGGGC
    GAGTGGCTCA GGAAGAAAAG AATTGTAACC GGAAATCCTC GCTGTCAAAA ACCATACTTC
    CTCAAAGAAA TCCCCATCCA GGACGTCGCC ATTCAAGACT TCACGTGTGA CGACGGAAAT
    GACGACAGTA GCTGTTCTCC ACTCTCGCGC TGTCCCACGG AATGCACGTG CTTGGATACA
    GTTGTCCGAT GTAGCAACAA GGGCCTGAAG GTCTTGCCCA AAGGTATTCC CAGAGACGTC
    ACTGAACTGT ATCTGGATGG GAACCACTTT ACCTTGGTTC CCAAGGAGCT CTATAACTAC
    AAACATCTAA CGCTTATAGA CCTGAGCAAC AACCGCATAA GCACTCTTTC TAATCAGAGC
    TTCAGCAACA TGACCCAGCT CCTCACCCTA ATTCTCAGTT ACAACCGTTT GAGATGTATT
    CCTCCTCGAA CCTTCGATGG ACTCAAGTCT CTCCGATTAC TTTCATTACA TGGAAATGAC
    ATTTCTGTTG TGCCTGAAGG TGCTTTCAGT GATCTCTCTG CATTATCACA CCTAGCAATC
    GGAGCCAACC CCCTTTACTG TGATTGCAAC ATGCAGTGGT TATCGGACTG GGTAAAGTCG
    GAATACAAAG AACCCGGGAT TGCTCGCTGT GCCGGCCCCG GAGAAATGGC AGATAAATTA
    TTACTCACGA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CTGTGGATAT CAATATTCTA
    GCTAAATGTA ATCCCTGCTT ATCAAACCCA TGTAAGAATG ATGGCACCTG TAACAATGAT
    CCAGTCGACT TTTATCGCTG TACCTGTCCG TATGGTTTCA AGGGGCAGGA CTGTGATGTC
    CCAATCCACG CATGCATCAG TAACCCGTGT ACACATGGAG GAACTTGCCA CTTAAAGGAG
    GGAGAAAAAG ATGGATTCTG GTGTATTTGT GCCGATGGAT TTGAAGGAGA AAATTGTGAA
    GTCAATGTTG ATGACTGTGA AGATAATGAC TGTGAAAATA ACTCTACGTG TGTCGATGGA
    ATTAATAACT ACACATGCCT TTGTCCGCCT GAGTACACAG GCGAGTTGTG TGAGGAGAAG
    CTGGACTTCT GCGCTCAGGA CCTGAACCCC TGCCAGCACG ACTCCAAGTG CATCCTGATG
    CCCAAAGGAT TCAAATGCGA CTGCACGCCG GGGTACGTGG GCGAGCACTG CGACATCGAC
    TTCGACGACT GCCAGGATCA CAAGTGTAAA AACGGAGCGC ACTGCACGGA CGCGGTGAAC
    GGCTACACGT GCACCTGCCC CGAAGGCTAC AGCGGCTTGT TCTGTGAATT CTCCCCGCCC
    ATGGTCCTCC CACGCACCAG CCCCTGTGAC AACTTCGACT GTCAGAACGG GGCGCAGTGC
    ATCGTCAGGG CGGGCGAGCC AATCTGCCAG TGTCTGCCCG GCTACCAGGG GGACAAGTGT
    GAGAAGTTGG TCAGCGTGAA CTTCGTGAAC AAAGAGTCGT ATCTTCAAAT TCCTTCAGCC
    AAGGTCCGGC CCCAAACGAA CATCACCCTG CAGATTGCCA CCGACGAAGA CAGCGGGATC
    CTCCTGTACA AGGGCGACAA GGACCACATT GCCGTGGAGC TGTATCGGGG ACGGGTGCGC
    GCCAGCTACG ACACCGGCTC GCACCCCGCT TCTGCCATTT ACAGCGTGGA GACGATCAAT
    GATGGAAACT TTCACATTGT GGAACTACTT GCCCTGGATC AGAGCCTGTC CCTCTCCGTG
    GATGGAGGGA GCCCCAAAAT CATCACCAAC TTGTCAAAGC AGTCCACTCT GAATTTTGAC
    TCTCCACTCT ATGTAGGAGG CATGCCCGGG AGGAACAACG TGGCCGCGGC CCTGCGCCAG
    GCCCCGGGGC ACAACGGCAC CAGCTTCCAC GGCTGCATCC GGAACCTGTA TATCAACAGC
    GAGCTCCAGG ACTTCCGCCA GGTGCCCATG CAGACCGGCA TCCTGCCCGG CTGCGAGCCG
    TGCCACAGGA AGGTGTGTGC CCACGGCGCG TGCCAGCCCA GCAGCCAGTC GGGCTTCACC
    TGCGAGTGCG AGGAGGGCTG GACGGGGCCC CTGTGTGACC AGAGGACCAA CGACCCCTGT
    CTCGGGAACA AATGTGTGCA CGGCACCTGC TTGCCCATCA ACGCCTTCTC CTACAGCTGT
    AAGTGTCTGG AGGGCCACGG GGGCGTCCTC TGCGACGAAG AGGAGGACCT GTTCAACCCC
    TGCCAGGCCA TCAGGTGCAA GCACGGGAAA TGCAGGCTCT CGGGCCTGGG CCAGCCCTAC
    TGCGAATGCA GCAGCGGGTA CACGGGGGAT AGCTGCGACC GAGAAGTGTC CTGTCGGGGC
    GAGCGCGTCC GGGACTACTA CCCAAAGCAG CAGGGCTACG CGGCCTGCCA GACCACCAAG
    AAGGTGTCGC GGCTGGAGTG CAGGGGCGGC TGCGCGGCCG GGCAGTGCTG CGGGCCGCTG
    CGGAGCAAGC GGCGGAAATA CTCCTTCGAG TGCACGGACG GCTCGTCGTT CGTGGACGAG
    GTGGAGAAGG TGGTCAAGTG CGGCTGCAGC AGGTGCGCCG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968146.1
    CDS86..4690
    Translation

    Target ORF information:

    RefSeq Version XM_014112671.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112671.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
    GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC
    TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG
    GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC
    CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG
    GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG
    TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG
    GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC
    AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC
    ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT
    TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG
    GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT
    TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG
    AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT
    CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC
    TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT
    GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC
    AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC
    TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC
    CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG
    TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA
    AGCGGGGACT GCTTTGCAGA TCTGGCTTGC CCTGAAAAGT GCCGCTGTGA AGGAACCACA
    GTAGATTGCT CCAATCAAAA ACTCACCAAA ATCCCAGACC ACATCCCCCA GTACACTGCA
    GAGCTGCGTC TCAATAATAA TGAATTCACA GTGCTGGAAG CTACAGGAAT CTTCAAGAAA
    CTTCCGCAGT TACGTAAAAT AAACTTCAGC AACAACAAGA TCACAGACAT TGAAGAAGGA
    GCATTTGAAG GAGCAGCTGG TGTAAACGAA ATCCTTCTCA CGAGTAACCG TTTGGAAAAT
    GTTCAGCATA AGATGTTCAA GGGATTGGAA AGCCTGAAAA CGTTGATGTT GCGAAGCAAT
    CGCATAAGCT GCGTTGGCAA CGATAGCTTC ATAGGCCTGA GCTCTGTGCG GTTGCTTTCG
    CTGTACGATA ATCAGATCGC CACCATCGCG CCGGGGGCGT TCGACACCCT GCACTCGTTG
    TCCACCCTAA ACCTGTTGGC CAACCCTTTT AACTGCAACT GCTACCTGGC TTGGCTGGGC
    GAGTGGCTCA GGAAGAAAAG AATTGTAACC GGAAATCCTC GCTGTCAAAA ACCATACTTC
    CTCAAAGAAA TCCCCATCCA GGACGTCGCC ATTCAAGACT TCACGTGTGA CGACGGAAAT
    GACGACAGTA GCTGTTCTCC ACTCTCGCGC TGTCCCACGG AATGCACGTG CTTGGATACA
    GTTGTCCGAT GTAGCAACAA GGGCCTGAAG GTCTTGCCCA AAGGTATTCC CAGAGACGTC
    ACTGAACTGT ATCTGGATGG GAACCACTTT ACCTTGGTTC CCAAGGAGCT CTATAACTAC
    AAACATCTAA CGCTTATAGA CCTGAGCAAC AACCGCATAA GCACTCTTTC TAATCAGAGC
    TTCAGCAACA TGACCCAGCT CCTCACCCTA ATTCTCAGTT ACAACCGTTT GAGATGTATT
    CCTCCTCGAA CCTTCGATGG ACTCAAGTCT CTCCGATTAC TTTCATTACA TGGAAATGAC
    ATTTCTGTTG TGCCTGAAGG TGCTTTCAGT GATCTCTCTG CATTATCACA CCTAGCAATC
    GGAGCCAACC CCCTTTACTG TGATTGCAAC ATGCAGTGGT TATCGGACTG GGTAAAGTCG
    GAATACAAAG AACCCGGGAT TGCTCGCTGT GCCGGCCCCG GAGAAATGGC AGATAAATTA
    TTACTCACGA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CTGTGGATAT CAATATTCTA
    GCTAAATGTA ATCCCTGCTT ATCAAACCCA TGTAAGAATG ATGGCACCTG TAACAATGAT
    CCAGTCGACT TTTATCGCTG TACCTGTCCG TATGGTTTCA AGGGGCAGGA CTGTGATGTC
    CCAATCCACG CATGCATCAG TAACCCGTGT ACACATGGAG GAACTTGCCA CTTAAAGGAG
    GGAGAAAAAG ATGGATTCTG GTGTATTTGT GCCGATGGAT TTGAAGGAGA AAATTGTGAA
    GTCAATGTTG ATGACTGTGA AGATAATGAC TGTGAAAATA ACTCTACGTG TGTCGATGGA
    ATTAATAACT ACACATGCCT TTGTCCGCCT GAGTACACAG GCGAGTTGTG TGAGGAGAAG
    CTGGACTTCT GCGCTCAGGA CCTGAACCCC TGCCAGCACG ACTCCAAGTG CATCCTGATG
    CCCAAAGGAT TCAAATGCGA CTGCACGCCG GGGTACGTGG GCGAGCACTG CGACATCGAC
    TTCGACGACT GCCAGGATCA CAAGTGTAAA AACGGAGCGC ACTGCACGGA CGCGGTGAAC
    GGCTACACGT GCACCTGCCC CGAAGGCTAC AGCGGCTTGT TCTGTGAATT CTCCCCGCCC
    ATGGTCCTCC CACGCACCAG CCCCTGTGAC AACTTCGACT GTCAGAACGG GGCGCAGTGC
    ATCGTCAGGG CGGGCGAGCC AATCTGCCAG TGTCTGCCCG GCTACCAGGG GGACAAGTGT
    GAGAAGTTGG TCAGCGTGAA CTTCGTGAAC AAAGAGTCGT ATCTTCAAAT TCCTTCAGCC
    AAGGTCCGGC CCCAAACGAA CATCACCCTG CAGATTGCCA CCGACGAAGA CAGCGGGATC
    CTCCTGTACA AGGGCGACAA GGACCACATT GCCGTGGAGC TGTATCGGGG ACGGGTGCGC
    GCCAGCTACG ACACCGGCTC GCACCCCGCT TCTGCCATTT ACAGCGTGGA GACGATCAAT
    GATGGAAACT TTCACATTGT GGAACTACTT GCCCTGGATC AGAGCCTGTC CCTCTCCGTG
    GATGGAGGGA GCCCCAAAAT CATCACCAAC TTGTCAAAGC AGTCCACTCT GAATTTTGAC
    TCTCCACTCT ATGTAGGAGG CATGCCCGGG AGGAACAACG TGGCCGCGGC CCTGCGCCAG
    GCCCCGGGGC ACAACGGCAC CAGCTTCCAC GGCTGCATCC GGAACCTGTA TATCAACAGC
    GAGCTCCAGG ACTTCCGCCA GGTGCCCATG CAGACCGGCA TCCTGCCCGG CTGCGAGCCG
    TGCCACAGGA AGGTGTGTGC CCACGGCGCG TGCCAGCCCA GCAGCCAGTC GGGCTTCACC
    TGCGAGTGCG AGGAGGGCTG GACGGGGCCC CTGTGTGACC AGAGGACCAA CGACCCCTGT
    CTCGGGAACA AATGTGTGCA CGGCACCTGC TTGCCCATCA ACGCCTTCTC CTACAGCTGT
    AAGTGTCTGG AGGGCCACGG GGGCGTCCTC TGCGACGAAG AGGAGGACCT GTTCAACCCC
    TGCCAGGCCA TCAGGTGCAA GCACGGGAAA TGCAGGCTCT CGGGCCTGGG CCAGCCCTAC
    TGCGAATGCA GCAGCGGGTA CACGGGGGAT AGCTGCGACC GAGAAGTGTC CTGTCGGGGC
    GAGCGCGTCC GGGACTACTA CCCAAAGCAG CAGGGCTACG CGGCCTGCCA GACCACCAAG
    AAGGTGTCGC GGCTGGAGTG CAGGGGCGGC TGCGCGGCCG GGCAGTGCTG CGGGCCGCTG
    CGGAGCAAGC GGCGGAAATA CTCCTTCGAG TGCACGGACG GCTCGTCGTT CGTGGACGAG
    GTGGAGAAGG TGGTCAAGTG CGGCTGCAGC AGGTGCGCCG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35885
    Clone ID Related Accession (Same CDS sequence) XM_014112671.1 , XM_014112671.2
    Accession Version XM_014112671.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product slit homolog 2 protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112671.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
    GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC
    TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG
    GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC
    CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG
    GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG
    TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG
    GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC
    AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC
    ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT
    TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG
    GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT
    TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG
    AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT
    CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC
    TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT
    GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC
    AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC
    TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC
    CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG
    TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA
    AGCGGGGACT GCTTTGCAGA TCTGGCTTGC CCTGAAAAGT GCCGCTGTGA AGGAACCACA
    GTAGATTGCT CCAATCAAAA ACTCACCAAA ATCCCAGACC ACATCCCCCA GTACACTGCA
    GAGCTGCGTC TCAATAATAA TGAATTCACA GTGCTGGAAG CTACAGGAAT CTTCAAGAAA
    CTTCCGCAGT TACGTAAAAT AAACTTCAGC AACAACAAGA TCACAGACAT TGAAGAAGGA
    GCATTTGAAG GAGCAGCTGG TGTAAACGAA ATCCTTCTCA CGAGTAACCG TTTGGAAAAT
    GTTCAGCATA AGATGTTCAA GGGATTGGAA AGCCTGAAAA CGTTGATGTT GCGAAGCAAT
    CGCATAAGCT GCGTTGGCAA CGATAGCTTC ATAGGCCTGA GCTCTGTGCG GTTGCTTTCG
    CTGTACGATA ATCAGATCGC CACCATCGCG CCGGGGGCGT TCGACACCCT GCACTCGTTG
    TCCACCCTAA ACCTGTTGGC CAACCCTTTT AACTGCAACT GCTACCTGGC TTGGCTGGGC
    GAGTGGCTCA GGAAGAAAAG AATTGTAACC GGAAATCCTC GCTGTCAAAA ACCATACTTC
    CTCAAAGAAA TCCCCATCCA GGACGTCGCC ATTCAAGACT TCACGTGTGA CGACGGAAAT
    GACGACAGTA GCTGTTCTCC ACTCTCGCGC TGTCCCACGG AATGCACGTG CTTGGATACA
    GTTGTCCGAT GTAGCAACAA GGGCCTGAAG GTCTTGCCCA AAGGTATTCC CAGAGACGTC
    ACTGAACTGT ATCTGGATGG GAACCACTTT ACCTTGGTTC CCAAGGAGCT CTATAACTAC
    AAACATCTAA CGCTTATAGA CCTGAGCAAC AACCGCATAA GCACTCTTTC TAATCAGAGC
    TTCAGCAACA TGACCCAGCT CCTCACCCTA ATTCTCAGTT ACAACCGTTT GAGATGTATT
    CCTCCTCGAA CCTTCGATGG ACTCAAGTCT CTCCGATTAC TTTCATTACA TGGAAATGAC
    ATTTCTGTTG TGCCTGAAGG TGCTTTCAGT GATCTCTCTG CATTATCACA CCTAGCAATC
    GGAGCCAACC CCCTTTACTG TGATTGCAAC ATGCAGTGGT TATCGGACTG GGTAAAGTCG
    GAATACAAAG AACCCGGGAT TGCTCGCTGT GCCGGCCCCG GAGAAATGGC AGATAAATTA
    TTACTCACGA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CTGTGGATAT CAATATTCTA
    GCTAAATGTA ATCCCTGCTT ATCAAACCCA TGTAAGAATG ATGGCACCTG TAACAATGAT
    CCAGTCGACT TTTATCGCTG TACCTGTCCG TATGGTTTCA AGGGGCAGGA CTGTGATGTC
    CCAATCCACG CATGCATCAG TAACCCGTGT ACACATGGAG GAACTTGCCA CTTAAAGGAG
    GGAGAAAAAG ATGGATTCTG GTGTATTTGT GCCGATGGAT TTGAAGGAGA AAATTGTGAA
    GTCAATGTTG ATGACTGTGA AGATAATGAC TGTGAAAATA ACTCTACGTG TGTCGATGGA
    ATTAATAACT ACACATGCCT TTGTCCGCCT GAGTACACAG GCGAGTTGTG TGAGGAGAAG
    CTGGACTTCT GCGCTCAGGA CCTGAACCCC TGCCAGCACG ACTCCAAGTG CATCCTGATG
    CCCAAAGGAT TCAAATGCGA CTGCACGCCG GGGTACGTGG GCGAGCACTG CGACATCGAC
    TTCGACGACT GCCAGGATCA CAAGTGTAAA AACGGAGCGC ACTGCACGGA CGCGGTGAAC
    GGCTACACGT GCACCTGCCC CGAAGGCTAC AGCGGCTTGT TCTGTGAATT CTCCCCGCCC
    ATGGTCCTCC CACGCACCAG CCCCTGTGAC AACTTCGACT GTCAGAACGG GGCGCAGTGC
    ATCGTCAGGG CGGGCGAGCC AATCTGCCAG TGTCTGCCCG GCTACCAGGG GGACAAGTGT
    GAGAAGTTGG TCAGCGTGAA CTTCGTGAAC AAAGAGTCGT ATCTTCAAAT TCCTTCAGCC
    AAGGTCCGGC CCCAAACGAA CATCACCCTG CAGATTGCCA CCGACGAAGA CAGCGGGATC
    CTCCTGTACA AGGGCGACAA GGACCACATT GCCGTGGAGC TGTATCGGGG ACGGGTGCGC
    GCCAGCTACG ACACCGGCTC GCACCCCGCT TCTGCCATTT ACAGCGTGGA GACGATCAAT
    GATGGAAACT TTCACATTGT GGAACTACTT GCCCTGGATC AGAGCCTGTC CCTCTCCGTG
    GATGGAGGGA GCCCCAAAAT CATCACCAAC TTGTCAAAGC AGTCCACTCT GAATTTTGAC
    TCTCCACTCT ATGTAGGAGG CATGCCCGGG AGGAACAACG TGGCCGCGGC CCTGCGCCAG
    GCCCCGGGGC ACAACGGCAC CAGCTTCCAC GGCTGCATCC GGAACCTGTA TATCAACAGC
    GAGCTCCAGG ACTTCCGCCA GGTGCCCATG CAGACCGGCA TCCTGCCCGG CTGCGAGCCG
    TGCCACAGGA AGGTGTGTGC CCACGGCGCG TGCCAGCCCA GCAGCCAGTC GGGCTTCACC
    TGCGAGTGCG AGGAGGGCTG GACGGGGCCC CTGTGTGACC AGAGGACCAA CGACCCCTGT
    CTCGGGAACA AATGTGTGCA CGGCACCTGC TTGCCCATCA ACGCCTTCTC CTACAGCTGT
    AAGTGTCTGG AGGGCCACGG GGGCGTCCTC TGCGACGAAG AGGAGGACCT GTTCAACCCC
    TGCCAGGCCA TCAGGTGCAA GCACGGGAAA TGCAGGCTCT CGGGCCTGGG CCAGCCCTAC
    TGCGAATGCA GCAGCGGGTA CACGGGGGAT AGCTGCGACC GAGAAGTGTC CTGTCGGGGC
    GAGCGCGTCC GGGACTACTA CCCAAAGCAG CAGGGCTACG CGGCCTGCCA GACCACCAAG
    AAGGTGTCGC GGCTGGAGTG CAGGGGCGGC TGCGCGGCCG GGCAGTGCTG CGGGCCGCTG
    CGGAGCAAGC GGCGGAAATA CTCCTTCGAG TGCACGGACG GCTCGTCGTT CGTGGACGAG
    GTGGAGAAGG TGGTCAAGTG CGGCTGCAGC AGGTGCGCCG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968146.1
    CDS212..4816
    Translation

    Target ORF information:

    RefSeq Version XM_014112671.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112671.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
    GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC
    TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG
    GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC
    CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG
    GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG
    TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG
    GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC
    AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC
    ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT
    TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG
    GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT
    TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG
    AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT
    CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC
    TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT
    GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC
    AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC
    TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC
    CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG
    TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA
    AGCGGGGACT GCTTTGCAGA TCTGGCTTGC CCTGAAAAGT GCCGCTGTGA AGGAACCACA
    GTAGATTGCT CCAATCAAAA ACTCACCAAA ATCCCAGACC ACATCCCCCA GTACACTGCA
    GAGCTGCGTC TCAATAATAA TGAATTCACA GTGCTGGAAG CTACAGGAAT CTTCAAGAAA
    CTTCCGCAGT TACGTAAAAT AAACTTCAGC AACAACAAGA TCACAGACAT TGAAGAAGGA
    GCATTTGAAG GAGCAGCTGG TGTAAACGAA ATCCTTCTCA CGAGTAACCG TTTGGAAAAT
    GTTCAGCATA AGATGTTCAA GGGATTGGAA AGCCTGAAAA CGTTGATGTT GCGAAGCAAT
    CGCATAAGCT GCGTTGGCAA CGATAGCTTC ATAGGCCTGA GCTCTGTGCG GTTGCTTTCG
    CTGTACGATA ATCAGATCGC CACCATCGCG CCGGGGGCGT TCGACACCCT GCACTCGTTG
    TCCACCCTAA ACCTGTTGGC CAACCCTTTT AACTGCAACT GCTACCTGGC TTGGCTGGGC
    GAGTGGCTCA GGAAGAAAAG AATTGTAACC GGAAATCCTC GCTGTCAAAA ACCATACTTC
    CTCAAAGAAA TCCCCATCCA GGACGTCGCC ATTCAAGACT TCACGTGTGA CGACGGAAAT
    GACGACAGTA GCTGTTCTCC ACTCTCGCGC TGTCCCACGG AATGCACGTG CTTGGATACA
    GTTGTCCGAT GTAGCAACAA GGGCCTGAAG GTCTTGCCCA AAGGTATTCC CAGAGACGTC
    ACTGAACTGT ATCTGGATGG GAACCACTTT ACCTTGGTTC CCAAGGAGCT CTATAACTAC
    AAACATCTAA CGCTTATAGA CCTGAGCAAC AACCGCATAA GCACTCTTTC TAATCAGAGC
    TTCAGCAACA TGACCCAGCT CCTCACCCTA ATTCTCAGTT ACAACCGTTT GAGATGTATT
    CCTCCTCGAA CCTTCGATGG ACTCAAGTCT CTCCGATTAC TTTCATTACA TGGAAATGAC
    ATTTCTGTTG TGCCTGAAGG TGCTTTCAGT GATCTCTCTG CATTATCACA CCTAGCAATC
    GGAGCCAACC CCCTTTACTG TGATTGCAAC ATGCAGTGGT TATCGGACTG GGTAAAGTCG
    GAATACAAAG AACCCGGGAT TGCTCGCTGT GCCGGCCCCG GAGAAATGGC AGATAAATTA
    TTACTCACGA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CTGTGGATAT CAATATTCTA
    GCTAAATGTA ATCCCTGCTT ATCAAACCCA TGTAAGAATG ATGGCACCTG TAACAATGAT
    CCAGTCGACT TTTATCGCTG TACCTGTCCG TATGGTTTCA AGGGGCAGGA CTGTGATGTC
    CCAATCCACG CATGCATCAG TAACCCGTGT ACACATGGAG GAACTTGCCA CTTAAAGGAG
    GGAGAAAAAG ATGGATTCTG GTGTATTTGT GCCGATGGAT TTGAAGGAGA AAATTGTGAA
    GTCAATGTTG ATGACTGTGA AGATAATGAC TGTGAAAATA ACTCTACGTG TGTCGATGGA
    ATTAATAACT ACACATGCCT TTGTCCGCCT GAGTACACAG GCGAGTTGTG TGAGGAGAAG
    CTGGACTTCT GCGCTCAGGA CCTGAACCCC TGCCAGCACG ACTCCAAGTG CATCCTGATG
    CCCAAAGGAT TCAAATGCGA CTGCACGCCG GGGTACGTGG GCGAGCACTG CGACATCGAC
    TTCGACGACT GCCAGGATCA CAAGTGTAAA AACGGAGCGC ACTGCACGGA CGCGGTGAAC
    GGCTACACGT GCACCTGCCC CGAAGGCTAC AGCGGCTTGT TCTGTGAATT CTCCCCGCCC
    ATGGTCCTCC CACGCACCAG CCCCTGTGAC AACTTCGACT GTCAGAACGG GGCGCAGTGC
    ATCGTCAGGG CGGGCGAGCC AATCTGCCAG TGTCTGCCCG GCTACCAGGG GGACAAGTGT
    GAGAAGTTGG TCAGCGTGAA CTTCGTGAAC AAAGAGTCGT ATCTTCAAAT TCCTTCAGCC
    AAGGTCCGGC CCCAAACGAA CATCACCCTG CAGATTGCCA CCGACGAAGA CAGCGGGATC
    CTCCTGTACA AGGGCGACAA GGACCACATT GCCGTGGAGC TGTATCGGGG ACGGGTGCGC
    GCCAGCTACG ACACCGGCTC GCACCCCGCT TCTGCCATTT ACAGCGTGGA GACGATCAAT
    GATGGAAACT TTCACATTGT GGAACTACTT GCCCTGGATC AGAGCCTGTC CCTCTCCGTG
    GATGGAGGGA GCCCCAAAAT CATCACCAAC TTGTCAAAGC AGTCCACTCT GAATTTTGAC
    TCTCCACTCT ATGTAGGAGG CATGCCCGGG AGGAACAACG TGGCCGCGGC CCTGCGCCAG
    GCCCCGGGGC ACAACGGCAC CAGCTTCCAC GGCTGCATCC GGAACCTGTA TATCAACAGC
    GAGCTCCAGG ACTTCCGCCA GGTGCCCATG CAGACCGGCA TCCTGCCCGG CTGCGAGCCG
    TGCCACAGGA AGGTGTGTGC CCACGGCGCG TGCCAGCCCA GCAGCCAGTC GGGCTTCACC
    TGCGAGTGCG AGGAGGGCTG GACGGGGCCC CTGTGTGACC AGAGGACCAA CGACCCCTGT
    CTCGGGAACA AATGTGTGCA CGGCACCTGC TTGCCCATCA ACGCCTTCTC CTACAGCTGT
    AAGTGTCTGG AGGGCCACGG GGGCGTCCTC TGCGACGAAG AGGAGGACCT GTTCAACCCC
    TGCCAGGCCA TCAGGTGCAA GCACGGGAAA TGCAGGCTCT CGGGCCTGGG CCAGCCCTAC
    TGCGAATGCA GCAGCGGGTA CACGGGGGAT AGCTGCGACC GAGAAGTGTC CTGTCGGGGC
    GAGCGCGTCC GGGACTACTA CCCAAAGCAG CAGGGCTACG CGGCCTGCCA GACCACCAAG
    AAGGTGTCGC GGCTGGAGTG CAGGGGCGGC TGCGCGGCCG GGCAGTGCTG CGGGCCGCTG
    CGGAGCAAGC GGCGGAAATA CTCCTTCGAG TGCACGGACG GCTCGTCGTT CGTGGACGAG
    GTGGAGAAGG TGGTCAAGTG CGGCTGCAGC AGGTGCGCCG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35886
    Clone ID Related Accession (Same CDS sequence) XM_014112672.2 , XM_014112672.1
    Accession Version XM_014112672.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4593bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product slit homolog 2 protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112672.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
    GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC
    TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG
    GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC
    CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG
    GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG
    TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG
    GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC
    AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC
    ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT
    TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGGCCAC
    CAGTCCTTCA TGGCTCCTTC TTGCAGCGTC CTGCATTGTC CAGCCGCTTG TACCTGTAGC
    AACAATATCG TAGACTGTCG TGGGAAAGGT CTCACTGAGA TCCCCACGAA CCTGCCAGAG
    ACCATCACAG AAATACGTTT GGAACAGAAC TCAATCAAGG TCATCCCTCC TGGAGCTTTC
    TCACCATATA AAAAGCTTAG AAGAATTGAC CTGAGCAATA ATCAGATCTC TGAACTAGCA
    CCGGACGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC
    ACGGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTGCT ATTATTGAAT
    GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ATCTGCACAA CCTGAATCTT
    CTCTCCCTGT ACGACAACAA GCTGCAGACC ATCGCCAAGG GGACCTTCTC ACCTCTCCGG
    GCCATTCAGA CCATGCACCT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG
    CTGGCGGACT ATCTCCACAC CAACCCCATC GAGACCAGTG GTGCCCGGTG CACCAGCCCC
    CGGCGCCTGG CAAACAAAAG AATCGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT
    AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG CGGGGACTGC
    TTTGCAGATC TGGCTTGCCC TGAAAAGTGC CGCTGTGAAG GAACCACAGT AGATTGCTCC
    AATCAAAAAC TCACCAAAAT CCCAGACCAC ATCCCCCAGT ACACTGCAGA GCTGCGTCTC
    AATAATAATG AATTCACAGT GCTGGAAGCT ACAGGAATCT TCAAGAAACT TCCGCAGTTA
    CGTAAAATAA ACTTCAGCAA CAACAAGATC ACAGACATTG AAGAAGGAGC ATTTGAAGGA
    GCAGCTGGTG TAAACGAAAT CCTTCTCACG AGTAACCGTT TGGAAAATGT TCAGCATAAG
    ATGTTCAAGG GATTGGAAAG CCTGAAAACG TTGATGTTGC GAAGCAATCG CATAAGCTGC
    GTTGGCAACG ATAGCTTCAT AGGCCTGAGC TCTGTGCGGT TGCTTTCGCT GTACGATAAT
    CAGATCGCCA CCATCGCGCC GGGGGCGTTC GACACCCTGC ACTCGTTGTC CACCCTAAAC
    CTGTTGGCCA ACCCTTTTAA CTGCAACTGC TACCTGGCTT GGCTGGGCGA GTGGCTCAGG
    AAGAAAAGAA TTGTAACCGG AAATCCTCGC TGTCAAAAAC CATACTTCCT CAAAGAAATC
    CCCATCCAGG ACGTCGCCAT TCAAGACTTC ACGTGTGACG ACGGAAATGA CGACAGTAGC
    TGTTCTCCAC TCTCGCGCTG TCCCACGGAA TGCACGTGCT TGGATACAGT TGTCCGATGT
    AGCAACAAGG GCCTGAAGGT CTTGCCCAAA GGTATTCCCA GAGACGTCAC TGAACTGTAT
    CTGGATGGGA ACCACTTTAC CTTGGTTCCC AAGGAGCTCT ATAACTACAA ACATCTAACG
    CTTATAGACC TGAGCAACAA CCGCATAAGC ACTCTTTCTA ATCAGAGCTT CAGCAACATG
    ACCCAGCTCC TCACCCTAAT TCTCAGTTAC AACCGTTTGA GATGTATTCC TCCTCGAACC
    TTCGATGGAC TCAAGTCTCT CCGATTACTT TCATTACATG GAAATGACAT TTCTGTTGTG
    CCTGAAGGTG CTTTCAGTGA TCTCTCTGCA TTATCACACC TAGCAATCGG AGCCAACCCC
    CTTTACTGTG ATTGCAACAT GCAGTGGTTA TCGGACTGGG TAAAGTCGGA ATACAAAGAA
    CCCGGGATTG CTCGCTGTGC CGGCCCCGGA GAAATGGCAG ATAAATTATT ACTCACGACT
    CCCTCCAAAA AATTTACATG TCAAGGTCCT GTGGATATCA ATATTCTAGC TAAATGTAAT
    CCCTGCTTAT CAAACCCATG TAAGAATGAT GGCACCTGTA ACAATGATCC AGTCGACTTT
    TATCGCTGTA CCTGTCCGTA TGGTTTCAAG GGGCAGGACT GTGATGTCCC AATCCACGCA
    TGCATCAGTA ACCCGTGTAC ACATGGAGGA ACTTGCCACT TAAAGGAGGG AGAAAAAGAT
    GGATTCTGGT GTATTTGTGC CGATGGATTT GAAGGAGAAA ATTGTGAAGT CAATGTTGAT
    GACTGTGAAG ATAATGACTG TGAAAATAAC TCTACGTGTG TCGATGGAAT TAATAACTAC
    ACATGCCTTT GTCCGCCTGA GTACACAGGC GAGTTGTGTG AGGAGAAGCT GGACTTCTGC
    GCTCAGGACC TGAACCCCTG CCAGCACGAC TCCAAGTGCA TCCTGATGCC CAAAGGATTC
    AAATGCGACT GCACGCCGGG GTACGTGGGC GAGCACTGCG ACATCGACTT CGACGACTGC
    CAGGATCACA AGTGTAAAAA CGGAGCGCAC TGCACGGACG CGGTGAACGG CTACACGTGC
    ACCTGCCCCG AAGGCTACAG CGGCTTGTTC TGTGAATTCT CCCCGCCCAT GGTCCTCCCA
    CGCACCAGCC CCTGTGACAA CTTCGACTGT CAGAACGGGG CGCAGTGCAT CGTCAGGGCG
    GGCGAGCCAA TCTGCCAGTG TCTGCCCGGC TACCAGGGGG ACAAGTGTGA GAAGTTGGTC
    AGCGTGAACT TCGTGAACAA AGAGTCGTAT CTTCAAATTC CTTCAGCCAA GGTCCGGCCC
    CAAACGAACA TCACCCTGCA GATTGCCACC GACGAAGACA GCGGGATCCT CCTGTACAAG
    GGCGACAAGG ACCACATTGC CGTGGAGCTG TATCGGGGAC GGGTGCGCGC CAGCTACGAC
    ACCGGCTCGC ACCCCGCTTC TGCCATTTAC AGCGTGGAGA CGATCAATGA TGGAAACTTT
    CACATTGTGG AACTACTTGC CCTGGATCAG AGCCTGTCCC TCTCCGTGGA TGGAGGGAGC
    CCCAAAATCA TCACCAACTT GTCAAAGCAG TCCACTCTGA ATTTTGACTC TCCACTCTAT
    GTAGGAGGCA TGCCCGGGAG GAACAACGTG GCCGCGGCCC TGCGCCAGGC CCCGGGGCAC
    AACGGCACCA GCTTCCACGG CTGCATCCGG AACCTGTATA TCAACAGCGA GCTCCAGGAC
    TTCCGCCAGG TGCCCATGCA GACCGGCATC CTGCCCGGCT GCGAGCCGTG CCACAGGAAG
    GTGTGTGCCC ACGGCGCGTG CCAGCCCAGC AGCCAGTCGG GCTTCACCTG CGAGTGCGAG
    GAGGGCTGGA CGGGGCCCCT GTGTGACCAG AGGACCAACG ACCCCTGTCT CGGGAACAAA
    TGTGTGCACG GCACCTGCTT GCCCATCAAC GCCTTCTCCT ACAGCTGTAA GTGTCTGGAG
    GGCCACGGGG GCGTCCTCTG CGACGAAGAG GAGGACCTGT TCAACCCCTG CCAGGCCATC
    AGGTGCAAGC ACGGGAAATG CAGGCTCTCG GGCCTGGGCC AGCCCTACTG CGAATGCAGC
    AGCGGGTACA CGGGGGATAG CTGCGACCGA GAAGTGTCCT GTCGGGGCGA GCGCGTCCGG
    GACTACTACC CAAAGCAGCA GGGCTACGCG GCCTGCCAGA CCACCAAGAA GGTGTCGCGG
    CTGGAGTGCA GGGGCGGCTG CGCGGCCGGG CAGTGCTGCG GGCCGCTGCG GAGCAAGCGG
    CGGAAATACT CCTTCGAGTG CACGGACGGC TCGTCGTTCG TGGACGAGGT GGAGAAGGTG
    GTCAAGTGCG GCTGCAGCAG GTGCGCCGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968147.1
    CDS212..4804
    Translation

    Target ORF information:

    RefSeq Version XM_014112672.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112672.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
    GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC
    TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG
    GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC
    CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG
    GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG
    TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG
    GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC
    AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC
    ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT
    TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGGCCAC
    CAGTCCTTCA TGGCTCCTTC TTGCAGCGTC CTGCATTGTC CAGCCGCTTG TACCTGTAGC
    AACAATATCG TAGACTGTCG TGGGAAAGGT CTCACTGAGA TCCCCACGAA CCTGCCAGAG
    ACCATCACAG AAATACGTTT GGAACAGAAC TCAATCAAGG TCATCCCTCC TGGAGCTTTC
    TCACCATATA AAAAGCTTAG AAGAATTGAC CTGAGCAATA ATCAGATCTC TGAACTAGCA
    CCGGACGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC
    ACGGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTGCT ATTATTGAAT
    GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ATCTGCACAA CCTGAATCTT
    CTCTCCCTGT ACGACAACAA GCTGCAGACC ATCGCCAAGG GGACCTTCTC ACCTCTCCGG
    GCCATTCAGA CCATGCACCT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG
    CTGGCGGACT ATCTCCACAC CAACCCCATC GAGACCAGTG GTGCCCGGTG CACCAGCCCC
    CGGCGCCTGG CAAACAAAAG AATCGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT
    AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG CGGGGACTGC
    TTTGCAGATC TGGCTTGCCC TGAAAAGTGC CGCTGTGAAG GAACCACAGT AGATTGCTCC
    AATCAAAAAC TCACCAAAAT CCCAGACCAC ATCCCCCAGT ACACTGCAGA GCTGCGTCTC
    AATAATAATG AATTCACAGT GCTGGAAGCT ACAGGAATCT TCAAGAAACT TCCGCAGTTA
    CGTAAAATAA ACTTCAGCAA CAACAAGATC ACAGACATTG AAGAAGGAGC ATTTGAAGGA
    GCAGCTGGTG TAAACGAAAT CCTTCTCACG AGTAACCGTT TGGAAAATGT TCAGCATAAG
    ATGTTCAAGG GATTGGAAAG CCTGAAAACG TTGATGTTGC GAAGCAATCG CATAAGCTGC
    GTTGGCAACG ATAGCTTCAT AGGCCTGAGC TCTGTGCGGT TGCTTTCGCT GTACGATAAT
    CAGATCGCCA CCATCGCGCC GGGGGCGTTC GACACCCTGC ACTCGTTGTC CACCCTAAAC
    CTGTTGGCCA ACCCTTTTAA CTGCAACTGC TACCTGGCTT GGCTGGGCGA GTGGCTCAGG
    AAGAAAAGAA TTGTAACCGG AAATCCTCGC TGTCAAAAAC CATACTTCCT CAAAGAAATC
    CCCATCCAGG ACGTCGCCAT TCAAGACTTC ACGTGTGACG ACGGAAATGA CGACAGTAGC
    TGTTCTCCAC TCTCGCGCTG TCCCACGGAA TGCACGTGCT TGGATACAGT TGTCCGATGT
    AGCAACAAGG GCCTGAAGGT CTTGCCCAAA GGTATTCCCA GAGACGTCAC TGAACTGTAT
    CTGGATGGGA ACCACTTTAC CTTGGTTCCC AAGGAGCTCT ATAACTACAA ACATCTAACG
    CTTATAGACC TGAGCAACAA CCGCATAAGC ACTCTTTCTA ATCAGAGCTT CAGCAACATG
    ACCCAGCTCC TCACCCTAAT TCTCAGTTAC AACCGTTTGA GATGTATTCC TCCTCGAACC
    TTCGATGGAC TCAAGTCTCT CCGATTACTT TCATTACATG GAAATGACAT TTCTGTTGTG
    CCTGAAGGTG CTTTCAGTGA TCTCTCTGCA TTATCACACC TAGCAATCGG AGCCAACCCC
    CTTTACTGTG ATTGCAACAT GCAGTGGTTA TCGGACTGGG TAAAGTCGGA ATACAAAGAA
    CCCGGGATTG CTCGCTGTGC CGGCCCCGGA GAAATGGCAG ATAAATTATT ACTCACGACT
    CCCTCCAAAA AATTTACATG TCAAGGTCCT GTGGATATCA ATATTCTAGC TAAATGTAAT
    CCCTGCTTAT CAAACCCATG TAAGAATGAT GGCACCTGTA ACAATGATCC AGTCGACTTT
    TATCGCTGTA CCTGTCCGTA TGGTTTCAAG GGGCAGGACT GTGATGTCCC AATCCACGCA
    TGCATCAGTA ACCCGTGTAC ACATGGAGGA ACTTGCCACT TAAAGGAGGG AGAAAAAGAT
    GGATTCTGGT GTATTTGTGC CGATGGATTT GAAGGAGAAA ATTGTGAAGT CAATGTTGAT
    GACTGTGAAG ATAATGACTG TGAAAATAAC TCTACGTGTG TCGATGGAAT TAATAACTAC
    ACATGCCTTT GTCCGCCTGA GTACACAGGC GAGTTGTGTG AGGAGAAGCT GGACTTCTGC
    GCTCAGGACC TGAACCCCTG CCAGCACGAC TCCAAGTGCA TCCTGATGCC CAAAGGATTC
    AAATGCGACT GCACGCCGGG GTACGTGGGC GAGCACTGCG ACATCGACTT CGACGACTGC
    CAGGATCACA AGTGTAAAAA CGGAGCGCAC TGCACGGACG CGGTGAACGG CTACACGTGC
    ACCTGCCCCG AAGGCTACAG CGGCTTGTTC TGTGAATTCT CCCCGCCCAT GGTCCTCCCA
    CGCACCAGCC CCTGTGACAA CTTCGACTGT CAGAACGGGG CGCAGTGCAT CGTCAGGGCG
    GGCGAGCCAA TCTGCCAGTG TCTGCCCGGC TACCAGGGGG ACAAGTGTGA GAAGTTGGTC
    AGCGTGAACT TCGTGAACAA AGAGTCGTAT CTTCAAATTC CTTCAGCCAA GGTCCGGCCC
    CAAACGAACA TCACCCTGCA GATTGCCACC GACGAAGACA GCGGGATCCT CCTGTACAAG
    GGCGACAAGG ACCACATTGC CGTGGAGCTG TATCGGGGAC GGGTGCGCGC CAGCTACGAC
    ACCGGCTCGC ACCCCGCTTC TGCCATTTAC AGCGTGGAGA CGATCAATGA TGGAAACTTT
    CACATTGTGG AACTACTTGC CCTGGATCAG AGCCTGTCCC TCTCCGTGGA TGGAGGGAGC
    CCCAAAATCA TCACCAACTT GTCAAAGCAG TCCACTCTGA ATTTTGACTC TCCACTCTAT
    GTAGGAGGCA TGCCCGGGAG GAACAACGTG GCCGCGGCCC TGCGCCAGGC CCCGGGGCAC
    AACGGCACCA GCTTCCACGG CTGCATCCGG AACCTGTATA TCAACAGCGA GCTCCAGGAC
    TTCCGCCAGG TGCCCATGCA GACCGGCATC CTGCCCGGCT GCGAGCCGTG CCACAGGAAG
    GTGTGTGCCC ACGGCGCGTG CCAGCCCAGC AGCCAGTCGG GCTTCACCTG CGAGTGCGAG
    GAGGGCTGGA CGGGGCCCCT GTGTGACCAG AGGACCAACG ACCCCTGTCT CGGGAACAAA
    TGTGTGCACG GCACCTGCTT GCCCATCAAC GCCTTCTCCT ACAGCTGTAA GTGTCTGGAG
    GGCCACGGGG GCGTCCTCTG CGACGAAGAG GAGGACCTGT TCAACCCCTG CCAGGCCATC
    AGGTGCAAGC ACGGGAAATG CAGGCTCTCG GGCCTGGGCC AGCCCTACTG CGAATGCAGC
    AGCGGGTACA CGGGGGATAG CTGCGACCGA GAAGTGTCCT GTCGGGGCGA GCGCGTCCGG
    GACTACTACC CAAAGCAGCA GGGCTACGCG GCCTGCCAGA CCACCAAGAA GGTGTCGCGG
    CTGGAGTGCA GGGGCGGCTG CGCGGCCGGG CAGTGCTGCG GGCCGCTGCG GAGCAAGCGG
    CGGAAATACT CCTTCGAGTG CACGGACGGC TCGTCGTTCG TGGACGAGGT GGAGAAGGTG
    GTCAAGTGCG GCTGCAGCAG GTGCGCCGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35886
    Clone ID Related Accession (Same CDS sequence) XM_014112672.2 , XM_014112672.1
    Accession Version XM_014112672.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4593bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product slit homolog 2 protein isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
    GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC
    TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG
    GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC
    CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG
    GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG
    TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG
    GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC
    AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC
    ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT
    TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGGCCAC
    CAGTCCTTCA TGGCTCCTTC TTGCAGCGTC CTGCATTGTC CAGCCGCTTG TACCTGTAGC
    AACAATATCG TAGACTGTCG TGGGAAAGGT CTCACTGAGA TCCCCACGAA CCTGCCAGAG
    ACCATCACAG AAATACGTTT GGAACAGAAC TCAATCAAGG TCATCCCTCC TGGAGCTTTC
    TCACCATATA AAAAGCTTAG AAGAATTGAC CTGAGCAATA ATCAGATCTC TGAACTAGCA
    CCGGACGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC
    ACGGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTGCT ATTATTGAAT
    GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ATCTGCACAA CCTGAATCTT
    CTCTCCCTGT ACGACAACAA GCTGCAGACC ATCGCCAAGG GGACCTTCTC ACCTCTCCGG
    GCCATTCAGA CCATGCACCT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG
    CTGGCGGACT ATCTCCACAC CAACCCCATC GAGACCAGTG GTGCCCGGTG CACCAGCCCC
    CGGCGCCTGG CAAACAAAAG AATCGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT
    AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG CGGGGACTGC
    TTTGCAGATC TGGCTTGCCC TGAAAAGTGC CGCTGTGAAG GAACCACAGT AGATTGCTCC
    AATCAAAAAC TCACCAAAAT CCCAGACCAC ATCCCCCAGT ACACTGCAGA GCTGCGTCTC
    AATAATAATG AATTCACAGT GCTGGAAGCT ACAGGAATCT TCAAGAAACT TCCGCAGTTA
    CGTAAAATAA ACTTCAGCAA CAACAAGATC ACAGACATTG AAGAAGGAGC ATTTGAAGGA
    GCAGCTGGTG TAAACGAAAT CCTTCTCACG AGTAACCGTT TGGAAAATGT TCAGCATAAG
    ATGTTCAAGG GATTGGAAAG CCTGAAAACG TTGATGTTGC GAAGCAATCG CATAAGCTGC
    GTTGGCAACG ATAGCTTCAT AGGCCTGAGC TCTGTGCGGT TGCTTTCGCT GTACGATAAT
    CAGATCGCCA CCATCGCGCC GGGGGCGTTC GACACCCTGC ACTCGTTGTC CACCCTAAAC
    CTGTTGGCCA ACCCTTTTAA CTGCAACTGC TACCTGGCTT GGCTGGGCGA GTGGCTCAGG
    AAGAAAAGAA TTGTAACCGG AAATCCTCGC TGTCAAAAAC CATACTTCCT CAAAGAAATC
    CCCATCCAGG ACGTCGCCAT TCAAGACTTC ACGTGTGACG ACGGAAATGA CGACAGTAGC
    TGTTCTCCAC TCTCGCGCTG TCCCACGGAA TGCACGTGCT TGGATACAGT TGTCCGATGT
    AGCAACAAGG GCCTGAAGGT CTTGCCCAAA GGTATTCCCA GAGACGTCAC TGAACTGTAT
    CTGGATGGGA ACCACTTTAC CTTGGTTCCC AAGGAGCTCT ATAACTACAA ACATCTAACG
    CTTATAGACC TGAGCAACAA CCGCATAAGC ACTCTTTCTA ATCAGAGCTT CAGCAACATG
    ACCCAGCTCC TCACCCTAAT TCTCAGTTAC AACCGTTTGA GATGTATTCC TCCTCGAACC
    TTCGATGGAC TCAAGTCTCT CCGATTACTT TCATTACATG GAAATGACAT TTCTGTTGTG
    CCTGAAGGTG CTTTCAGTGA TCTCTCTGCA TTATCACACC TAGCAATCGG AGCCAACCCC
    CTTTACTGTG ATTGCAACAT GCAGTGGTTA TCGGACTGGG TAAAGTCGGA ATACAAAGAA
    CCCGGGATTG CTCGCTGTGC CGGCCCCGGA GAAATGGCAG ATAAATTATT ACTCACGACT
    CCCTCCAAAA AATTTACATG TCAAGGTCCT GTGGATATCA ATATTCTAGC TAAATGTAAT
    CCCTGCTTAT CAAACCCATG TAAGAATGAT GGCACCTGTA ACAATGATCC AGTCGACTTT
    TATCGCTGTA CCTGTCCGTA TGGTTTCAAG GGGCAGGACT GTGATGTCCC AATCCACGCA
    TGCATCAGTA ACCCGTGTAC ACATGGAGGA ACTTGCCACT TAAAGGAGGG AGAAAAAGAT
    GGATTCTGGT GTATTTGTGC CGATGGATTT GAAGGAGAAA ATTGTGAAGT CAATGTTGAT
    GACTGTGAAG ATAATGACTG TGAAAATAAC TCTACGTGTG TCGATGGAAT TAATAACTAC
    ACATGCCTTT GTCCGCCTGA GTACACAGGC GAGTTGTGTG AGGAGAAGCT GGACTTCTGC
    GCTCAGGACC TGAACCCCTG CCAGCACGAC TCCAAGTGCA TCCTGATGCC CAAAGGATTC
    AAATGCGACT GCACGCCGGG GTACGTGGGC GAGCACTGCG ACATCGACTT CGACGACTGC
    CAGGATCACA AGTGTAAAAA CGGAGCGCAC TGCACGGACG CGGTGAACGG CTACACGTGC
    ACCTGCCCCG AAGGCTACAG CGGCTTGTTC TGTGAATTCT CCCCGCCCAT GGTCCTCCCA
    CGCACCAGCC CCTGTGACAA CTTCGACTGT CAGAACGGGG CGCAGTGCAT CGTCAGGGCG
    GGCGAGCCAA TCTGCCAGTG TCTGCCCGGC TACCAGGGGG ACAAGTGTGA GAAGTTGGTC
    AGCGTGAACT TCGTGAACAA AGAGTCGTAT CTTCAAATTC CTTCAGCCAA GGTCCGGCCC
    CAAACGAACA TCACCCTGCA GATTGCCACC GACGAAGACA GCGGGATCCT CCTGTACAAG
    GGCGACAAGG ACCACATTGC CGTGGAGCTG TATCGGGGAC GGGTGCGCGC CAGCTACGAC
    ACCGGCTCGC ACCCCGCTTC TGCCATTTAC AGCGTGGAGA CGATCAATGA TGGAAACTTT
    CACATTGTGG AACTACTTGC CCTGGATCAG AGCCTGTCCC TCTCCGTGGA TGGAGGGAGC
    CCCAAAATCA TCACCAACTT GTCAAAGCAG TCCACTCTGA ATTTTGACTC TCCACTCTAT
    GTAGGAGGCA TGCCCGGGAG GAACAACGTG GCCGCGGCCC TGCGCCAGGC CCCGGGGCAC
    AACGGCACCA GCTTCCACGG CTGCATCCGG AACCTGTATA TCAACAGCGA GCTCCAGGAC
    TTCCGCCAGG TGCCCATGCA GACCGGCATC CTGCCCGGCT GCGAGCCGTG CCACAGGAAG
    GTGTGTGCCC ACGGCGCGTG CCAGCCCAGC AGCCAGTCGG GCTTCACCTG CGAGTGCGAG
    GAGGGCTGGA CGGGGCCCCT GTGTGACCAG AGGACCAACG ACCCCTGTCT CGGGAACAAA
    TGTGTGCACG GCACCTGCTT GCCCATCAAC GCCTTCTCCT ACAGCTGTAA GTGTCTGGAG
    GGCCACGGGG GCGTCCTCTG CGACGAAGAG GAGGACCTGT TCAACCCCTG CCAGGCCATC
    AGGTGCAAGC ACGGGAAATG CAGGCTCTCG GGCCTGGGCC AGCCCTACTG CGAATGCAGC
    AGCGGGTACA CGGGGGATAG CTGCGACCGA GAAGTGTCCT GTCGGGGCGA GCGCGTCCGG
    GACTACTACC CAAAGCAGCA GGGCTACGCG GCCTGCCAGA CCACCAAGAA GGTGTCGCGG
    CTGGAGTGCA GGGGCGGCTG CGCGGCCGGG CAGTGCTGCG GGCCGCTGCG GAGCAAGCGG
    CGGAAATACT CCTTCGAGTG CACGGACGGC TCGTCGTTCG TGGACGAGGT GGAGAAGGTG
    GTCAAGTGCG GCTGCAGCAG GTGCGCCGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968147.1
    CDS86..4678
    Translation

    Target ORF information:

    RefSeq Version XM_014112672.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112672.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
    GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC
    TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG
    GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC
    CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG
    GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG
    TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG
    GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC
    AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC
    ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT
    TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGGCCAC
    CAGTCCTTCA TGGCTCCTTC TTGCAGCGTC CTGCATTGTC CAGCCGCTTG TACCTGTAGC
    AACAATATCG TAGACTGTCG TGGGAAAGGT CTCACTGAGA TCCCCACGAA CCTGCCAGAG
    ACCATCACAG AAATACGTTT GGAACAGAAC TCAATCAAGG TCATCCCTCC TGGAGCTTTC
    TCACCATATA AAAAGCTTAG AAGAATTGAC CTGAGCAATA ATCAGATCTC TGAACTAGCA
    CCGGACGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC
    ACGGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTGCT ATTATTGAAT
    GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ATCTGCACAA CCTGAATCTT
    CTCTCCCTGT ACGACAACAA GCTGCAGACC ATCGCCAAGG GGACCTTCTC ACCTCTCCGG
    GCCATTCAGA CCATGCACCT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG
    CTGGCGGACT ATCTCCACAC CAACCCCATC GAGACCAGTG GTGCCCGGTG CACCAGCCCC
    CGGCGCCTGG CAAACAAAAG AATCGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT
    AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG CGGGGACTGC
    TTTGCAGATC TGGCTTGCCC TGAAAAGTGC CGCTGTGAAG GAACCACAGT AGATTGCTCC
    AATCAAAAAC TCACCAAAAT CCCAGACCAC ATCCCCCAGT ACACTGCAGA GCTGCGTCTC
    AATAATAATG AATTCACAGT GCTGGAAGCT ACAGGAATCT TCAAGAAACT TCCGCAGTTA
    CGTAAAATAA ACTTCAGCAA CAACAAGATC ACAGACATTG AAGAAGGAGC ATTTGAAGGA
    GCAGCTGGTG TAAACGAAAT CCTTCTCACG AGTAACCGTT TGGAAAATGT TCAGCATAAG
    ATGTTCAAGG GATTGGAAAG CCTGAAAACG TTGATGTTGC GAAGCAATCG CATAAGCTGC
    GTTGGCAACG ATAGCTTCAT AGGCCTGAGC TCTGTGCGGT TGCTTTCGCT GTACGATAAT
    CAGATCGCCA CCATCGCGCC GGGGGCGTTC GACACCCTGC ACTCGTTGTC CACCCTAAAC
    CTGTTGGCCA ACCCTTTTAA CTGCAACTGC TACCTGGCTT GGCTGGGCGA GTGGCTCAGG
    AAGAAAAGAA TTGTAACCGG AAATCCTCGC TGTCAAAAAC CATACTTCCT CAAAGAAATC
    CCCATCCAGG ACGTCGCCAT TCAAGACTTC ACGTGTGACG ACGGAAATGA CGACAGTAGC
    TGTTCTCCAC TCTCGCGCTG TCCCACGGAA TGCACGTGCT TGGATACAGT TGTCCGATGT
    AGCAACAAGG GCCTGAAGGT CTTGCCCAAA GGTATTCCCA GAGACGTCAC TGAACTGTAT
    CTGGATGGGA ACCACTTTAC CTTGGTTCCC AAGGAGCTCT ATAACTACAA ACATCTAACG
    CTTATAGACC TGAGCAACAA CCGCATAAGC ACTCTTTCTA ATCAGAGCTT CAGCAACATG
    ACCCAGCTCC TCACCCTAAT TCTCAGTTAC AACCGTTTGA GATGTATTCC TCCTCGAACC
    TTCGATGGAC TCAAGTCTCT CCGATTACTT TCATTACATG GAAATGACAT TTCTGTTGTG
    CCTGAAGGTG CTTTCAGTGA TCTCTCTGCA TTATCACACC TAGCAATCGG AGCCAACCCC
    CTTTACTGTG ATTGCAACAT GCAGTGGTTA TCGGACTGGG TAAAGTCGGA ATACAAAGAA
    CCCGGGATTG CTCGCTGTGC CGGCCCCGGA GAAATGGCAG ATAAATTATT ACTCACGACT
    CCCTCCAAAA AATTTACATG TCAAGGTCCT GTGGATATCA ATATTCTAGC TAAATGTAAT
    CCCTGCTTAT CAAACCCATG TAAGAATGAT GGCACCTGTA ACAATGATCC AGTCGACTTT
    TATCGCTGTA CCTGTCCGTA TGGTTTCAAG GGGCAGGACT GTGATGTCCC AATCCACGCA
    TGCATCAGTA ACCCGTGTAC ACATGGAGGA ACTTGCCACT TAAAGGAGGG AGAAAAAGAT
    GGATTCTGGT GTATTTGTGC CGATGGATTT GAAGGAGAAA ATTGTGAAGT CAATGTTGAT
    GACTGTGAAG ATAATGACTG TGAAAATAAC TCTACGTGTG TCGATGGAAT TAATAACTAC
    ACATGCCTTT GTCCGCCTGA GTACACAGGC GAGTTGTGTG AGGAGAAGCT GGACTTCTGC
    GCTCAGGACC TGAACCCCTG CCAGCACGAC TCCAAGTGCA TCCTGATGCC CAAAGGATTC
    AAATGCGACT GCACGCCGGG GTACGTGGGC GAGCACTGCG ACATCGACTT CGACGACTGC
    CAGGATCACA AGTGTAAAAA CGGAGCGCAC TGCACGGACG CGGTGAACGG CTACACGTGC
    ACCTGCCCCG AAGGCTACAG CGGCTTGTTC TGTGAATTCT CCCCGCCCAT GGTCCTCCCA
    CGCACCAGCC CCTGTGACAA CTTCGACTGT CAGAACGGGG CGCAGTGCAT CGTCAGGGCG
    GGCGAGCCAA TCTGCCAGTG TCTGCCCGGC TACCAGGGGG ACAAGTGTGA GAAGTTGGTC
    AGCGTGAACT TCGTGAACAA AGAGTCGTAT CTTCAAATTC CTTCAGCCAA GGTCCGGCCC
    CAAACGAACA TCACCCTGCA GATTGCCACC GACGAAGACA GCGGGATCCT CCTGTACAAG
    GGCGACAAGG ACCACATTGC CGTGGAGCTG TATCGGGGAC GGGTGCGCGC CAGCTACGAC
    ACCGGCTCGC ACCCCGCTTC TGCCATTTAC AGCGTGGAGA CGATCAATGA TGGAAACTTT
    CACATTGTGG AACTACTTGC CCTGGATCAG AGCCTGTCCC TCTCCGTGGA TGGAGGGAGC
    CCCAAAATCA TCACCAACTT GTCAAAGCAG TCCACTCTGA ATTTTGACTC TCCACTCTAT
    GTAGGAGGCA TGCCCGGGAG GAACAACGTG GCCGCGGCCC TGCGCCAGGC CCCGGGGCAC
    AACGGCACCA GCTTCCACGG CTGCATCCGG AACCTGTATA TCAACAGCGA GCTCCAGGAC
    TTCCGCCAGG TGCCCATGCA GACCGGCATC CTGCCCGGCT GCGAGCCGTG CCACAGGAAG
    GTGTGTGCCC ACGGCGCGTG CCAGCCCAGC AGCCAGTCGG GCTTCACCTG CGAGTGCGAG
    GAGGGCTGGA CGGGGCCCCT GTGTGACCAG AGGACCAACG ACCCCTGTCT CGGGAACAAA
    TGTGTGCACG GCACCTGCTT GCCCATCAAC GCCTTCTCCT ACAGCTGTAA GTGTCTGGAG
    GGCCACGGGG GCGTCCTCTG CGACGAAGAG GAGGACCTGT TCAACCCCTG CCAGGCCATC
    AGGTGCAAGC ACGGGAAATG CAGGCTCTCG GGCCTGGGCC AGCCCTACTG CGAATGCAGC
    AGCGGGTACA CGGGGGATAG CTGCGACCGA GAAGTGTCCT GTCGGGGCGA GCGCGTCCGG
    GACTACTACC CAAAGCAGCA GGGCTACGCG GCCTGCCAGA CCACCAAGAA GGTGTCGCGG
    CTGGAGTGCA GGGGCGGCTG CGCGGCCGGG CAGTGCTGCG GGCCGCTGCG GAGCAAGCGG
    CGGAAATACT CCTTCGAGTG CACGGACGGC TCGTCGTTCG TGGACGAGGT GGAGAAGGTG
    GTCAAGTGCG GCTGCAGCAG GTGCGCCGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35887
    Clone ID Related Accession (Same CDS sequence) XM_014112673.1 , XM_014112673.2
    Accession Version XM_014112673.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4581bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product slit homolog 2 protein isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
    GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC
    TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG
    GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC
    CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG
    GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG
    TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG
    GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC
    AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC
    ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT
    TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG
    GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT
    TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG
    AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT
    CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC
    TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT
    GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC
    AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC
    TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC
    CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG
    TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG GTACAGAAGA TTATCGATCA AAATTAAGCG GGGACTGCTT TGCAGATCTG
    GCTTGCCCTG AAAAGTGCCG CTGTGAAGGA ACCACAGTAG ATTGCTCCAA TCAAAAACTC
    ACCAAAATCC CAGACCACAT CCCCCAGTAC ACTGCAGAGC TGCGTCTCAA TAATAATGAA
    TTCACAGTGC TGGAAGCTAC AGGAATCTTC AAGAAACTTC CGCAGTTACG TAAAATAAAC
    TTCAGCAACA ACAAGATCAC AGACATTGAA GAAGGAGCAT TTGAAGGAGC AGCTGGTGTA
    AACGAAATCC TTCTCACGAG TAACCGTTTG GAAAATGTTC AGCATAAGAT GTTCAAGGGA
    TTGGAAAGCC TGAAAACGTT GATGTTGCGA AGCAATCGCA TAAGCTGCGT TGGCAACGAT
    AGCTTCATAG GCCTGAGCTC TGTGCGGTTG CTTTCGCTGT ACGATAATCA GATCGCCACC
    ATCGCGCCGG GGGCGTTCGA CACCCTGCAC TCGTTGTCCA CCCTAAACCT GTTGGCCAAC
    CCTTTTAACT GCAACTGCTA CCTGGCTTGG CTGGGCGAGT GGCTCAGGAA GAAAAGAATT
    GTAACCGGAA ATCCTCGCTG TCAAAAACCA TACTTCCTCA AAGAAATCCC CATCCAGGAC
    GTCGCCATTC AAGACTTCAC GTGTGACGAC GGAAATGACG ACAGTAGCTG TTCTCCACTC
    TCGCGCTGTC CCACGGAATG CACGTGCTTG GATACAGTTG TCCGATGTAG CAACAAGGGC
    CTGAAGGTCT TGCCCAAAGG TATTCCCAGA GACGTCACTG AACTGTATCT GGATGGGAAC
    CACTTTACCT TGGTTCCCAA GGAGCTCTAT AACTACAAAC ATCTAACGCT TATAGACCTG
    AGCAACAACC GCATAAGCAC TCTTTCTAAT CAGAGCTTCA GCAACATGAC CCAGCTCCTC
    ACCCTAATTC TCAGTTACAA CCGTTTGAGA TGTATTCCTC CTCGAACCTT CGATGGACTC
    AAGTCTCTCC GATTACTTTC ATTACATGGA AATGACATTT CTGTTGTGCC TGAAGGTGCT
    TTCAGTGATC TCTCTGCATT ATCACACCTA GCAATCGGAG CCAACCCCCT TTACTGTGAT
    TGCAACATGC AGTGGTTATC GGACTGGGTA AAGTCGGAAT ACAAAGAACC CGGGATTGCT
    CGCTGTGCCG GCCCCGGAGA AATGGCAGAT AAATTATTAC TCACGACTCC CTCCAAAAAA
    TTTACATGTC AAGGTCCTGT GGATATCAAT ATTCTAGCTA AATGTAATCC CTGCTTATCA
    AACCCATGTA AGAATGATGG CACCTGTAAC AATGATCCAG TCGACTTTTA TCGCTGTACC
    TGTCCGTATG GTTTCAAGGG GCAGGACTGT GATGTCCCAA TCCACGCATG CATCAGTAAC
    CCGTGTACAC ATGGAGGAAC TTGCCACTTA AAGGAGGGAG AAAAAGATGG ATTCTGGTGT
    ATTTGTGCCG ATGGATTTGA AGGAGAAAAT TGTGAAGTCA ATGTTGATGA CTGTGAAGAT
    AATGACTGTG AAAATAACTC TACGTGTGTC GATGGAATTA ATAACTACAC ATGCCTTTGT
    CCGCCTGAGT ACACAGGCGA GTTGTGTGAG GAGAAGCTGG ACTTCTGCGC TCAGGACCTG
    AACCCCTGCC AGCACGACTC CAAGTGCATC CTGATGCCCA AAGGATTCAA ATGCGACTGC
    ACGCCGGGGT ACGTGGGCGA GCACTGCGAC ATCGACTTCG ACGACTGCCA GGATCACAAG
    TGTAAAAACG GAGCGCACTG CACGGACGCG GTGAACGGCT ACACGTGCAC CTGCCCCGAA
    GGCTACAGCG GCTTGTTCTG TGAATTCTCC CCGCCCATGG TCCTCCCACG CACCAGCCCC
    TGTGACAACT TCGACTGTCA GAACGGGGCG CAGTGCATCG TCAGGGCGGG CGAGCCAATC
    TGCCAGTGTC TGCCCGGCTA CCAGGGGGAC AAGTGTGAGA AGTTGGTCAG CGTGAACTTC
    GTGAACAAAG AGTCGTATCT TCAAATTCCT TCAGCCAAGG TCCGGCCCCA AACGAACATC
    ACCCTGCAGA TTGCCACCGA CGAAGACAGC GGGATCCTCC TGTACAAGGG CGACAAGGAC
    CACATTGCCG TGGAGCTGTA TCGGGGACGG GTGCGCGCCA GCTACGACAC CGGCTCGCAC
    CCCGCTTCTG CCATTTACAG CGTGGAGACG ATCAATGATG GAAACTTTCA CATTGTGGAA
    CTACTTGCCC TGGATCAGAG CCTGTCCCTC TCCGTGGATG GAGGGAGCCC CAAAATCATC
    ACCAACTTGT CAAAGCAGTC CACTCTGAAT TTTGACTCTC CACTCTATGT AGGAGGCATG
    CCCGGGAGGA ACAACGTGGC CGCGGCCCTG CGCCAGGCCC CGGGGCACAA CGGCACCAGC
    TTCCACGGCT GCATCCGGAA CCTGTATATC AACAGCGAGC TCCAGGACTT CCGCCAGGTG
    CCCATGCAGA CCGGCATCCT GCCCGGCTGC GAGCCGTGCC ACAGGAAGGT GTGTGCCCAC
    GGCGCGTGCC AGCCCAGCAG CCAGTCGGGC TTCACCTGCG AGTGCGAGGA GGGCTGGACG
    GGGCCCCTGT GTGACCAGAG GACCAACGAC CCCTGTCTCG GGAACAAATG TGTGCACGGC
    ACCTGCTTGC CCATCAACGC CTTCTCCTAC AGCTGTAAGT GTCTGGAGGG CCACGGGGGC
    GTCCTCTGCG ACGAAGAGGA GGACCTGTTC AACCCCTGCC AGGCCATCAG GTGCAAGCAC
    GGGAAATGCA GGCTCTCGGG CCTGGGCCAG CCCTACTGCG AATGCAGCAG CGGGTACACG
    GGGGATAGCT GCGACCGAGA AGTGTCCTGT CGGGGCGAGC GCGTCCGGGA CTACTACCCA
    AAGCAGCAGG GCTACGCGGC CTGCCAGACC ACCAAGAAGG TGTCGCGGCT GGAGTGCAGG
    GGCGGCTGCG CGGCCGGGCA GTGCTGCGGG CCGCTGCGGA GCAAGCGGCG GAAATACTCC
    TTCGAGTGCA CGGACGGCTC GTCGTTCGTG GACGAGGTGG AGAAGGTGGT CAAGTGCGGC
    TGCAGCAGGT GCGCCGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968148.1
    CDS86..4666
    Translation

    Target ORF information:

    RefSeq Version XM_014112673.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112673.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
    GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC
    TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG
    GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC
    CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG
    GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG
    TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG
    GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC
    AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC
    ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT
    TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG
    GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT
    TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG
    AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT
    CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC
    TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT
    GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC
    AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC
    TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC
    CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG
    TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG GTACAGAAGA TTATCGATCA AAATTAAGCG GGGACTGCTT TGCAGATCTG
    GCTTGCCCTG AAAAGTGCCG CTGTGAAGGA ACCACAGTAG ATTGCTCCAA TCAAAAACTC
    ACCAAAATCC CAGACCACAT CCCCCAGTAC ACTGCAGAGC TGCGTCTCAA TAATAATGAA
    TTCACAGTGC TGGAAGCTAC AGGAATCTTC AAGAAACTTC CGCAGTTACG TAAAATAAAC
    TTCAGCAACA ACAAGATCAC AGACATTGAA GAAGGAGCAT TTGAAGGAGC AGCTGGTGTA
    AACGAAATCC TTCTCACGAG TAACCGTTTG GAAAATGTTC AGCATAAGAT GTTCAAGGGA
    TTGGAAAGCC TGAAAACGTT GATGTTGCGA AGCAATCGCA TAAGCTGCGT TGGCAACGAT
    AGCTTCATAG GCCTGAGCTC TGTGCGGTTG CTTTCGCTGT ACGATAATCA GATCGCCACC
    ATCGCGCCGG GGGCGTTCGA CACCCTGCAC TCGTTGTCCA CCCTAAACCT GTTGGCCAAC
    CCTTTTAACT GCAACTGCTA CCTGGCTTGG CTGGGCGAGT GGCTCAGGAA GAAAAGAATT
    GTAACCGGAA ATCCTCGCTG TCAAAAACCA TACTTCCTCA AAGAAATCCC CATCCAGGAC
    GTCGCCATTC AAGACTTCAC GTGTGACGAC GGAAATGACG ACAGTAGCTG TTCTCCACTC
    TCGCGCTGTC CCACGGAATG CACGTGCTTG GATACAGTTG TCCGATGTAG CAACAAGGGC
    CTGAAGGTCT TGCCCAAAGG TATTCCCAGA GACGTCACTG AACTGTATCT GGATGGGAAC
    CACTTTACCT TGGTTCCCAA GGAGCTCTAT AACTACAAAC ATCTAACGCT TATAGACCTG
    AGCAACAACC GCATAAGCAC TCTTTCTAAT CAGAGCTTCA GCAACATGAC CCAGCTCCTC
    ACCCTAATTC TCAGTTACAA CCGTTTGAGA TGTATTCCTC CTCGAACCTT CGATGGACTC
    AAGTCTCTCC GATTACTTTC ATTACATGGA AATGACATTT CTGTTGTGCC TGAAGGTGCT
    TTCAGTGATC TCTCTGCATT ATCACACCTA GCAATCGGAG CCAACCCCCT TTACTGTGAT
    TGCAACATGC AGTGGTTATC GGACTGGGTA AAGTCGGAAT ACAAAGAACC CGGGATTGCT
    CGCTGTGCCG GCCCCGGAGA AATGGCAGAT AAATTATTAC TCACGACTCC CTCCAAAAAA
    TTTACATGTC AAGGTCCTGT GGATATCAAT ATTCTAGCTA AATGTAATCC CTGCTTATCA
    AACCCATGTA AGAATGATGG CACCTGTAAC AATGATCCAG TCGACTTTTA TCGCTGTACC
    TGTCCGTATG GTTTCAAGGG GCAGGACTGT GATGTCCCAA TCCACGCATG CATCAGTAAC
    CCGTGTACAC ATGGAGGAAC TTGCCACTTA AAGGAGGGAG AAAAAGATGG ATTCTGGTGT
    ATTTGTGCCG ATGGATTTGA AGGAGAAAAT TGTGAAGTCA ATGTTGATGA CTGTGAAGAT
    AATGACTGTG AAAATAACTC TACGTGTGTC GATGGAATTA ATAACTACAC ATGCCTTTGT
    CCGCCTGAGT ACACAGGCGA GTTGTGTGAG GAGAAGCTGG ACTTCTGCGC TCAGGACCTG
    AACCCCTGCC AGCACGACTC CAAGTGCATC CTGATGCCCA AAGGATTCAA ATGCGACTGC
    ACGCCGGGGT ACGTGGGCGA GCACTGCGAC ATCGACTTCG ACGACTGCCA GGATCACAAG
    TGTAAAAACG GAGCGCACTG CACGGACGCG GTGAACGGCT ACACGTGCAC CTGCCCCGAA
    GGCTACAGCG GCTTGTTCTG TGAATTCTCC CCGCCCATGG TCCTCCCACG CACCAGCCCC
    TGTGACAACT TCGACTGTCA GAACGGGGCG CAGTGCATCG TCAGGGCGGG CGAGCCAATC
    TGCCAGTGTC TGCCCGGCTA CCAGGGGGAC AAGTGTGAGA AGTTGGTCAG CGTGAACTTC
    GTGAACAAAG AGTCGTATCT TCAAATTCCT TCAGCCAAGG TCCGGCCCCA AACGAACATC
    ACCCTGCAGA TTGCCACCGA CGAAGACAGC GGGATCCTCC TGTACAAGGG CGACAAGGAC
    CACATTGCCG TGGAGCTGTA TCGGGGACGG GTGCGCGCCA GCTACGACAC CGGCTCGCAC
    CCCGCTTCTG CCATTTACAG CGTGGAGACG ATCAATGATG GAAACTTTCA CATTGTGGAA
    CTACTTGCCC TGGATCAGAG CCTGTCCCTC TCCGTGGATG GAGGGAGCCC CAAAATCATC
    ACCAACTTGT CAAAGCAGTC CACTCTGAAT TTTGACTCTC CACTCTATGT AGGAGGCATG
    CCCGGGAGGA ACAACGTGGC CGCGGCCCTG CGCCAGGCCC CGGGGCACAA CGGCACCAGC
    TTCCACGGCT GCATCCGGAA CCTGTATATC AACAGCGAGC TCCAGGACTT CCGCCAGGTG
    CCCATGCAGA CCGGCATCCT GCCCGGCTGC GAGCCGTGCC ACAGGAAGGT GTGTGCCCAC
    GGCGCGTGCC AGCCCAGCAG CCAGTCGGGC TTCACCTGCG AGTGCGAGGA GGGCTGGACG
    GGGCCCCTGT GTGACCAGAG GACCAACGAC CCCTGTCTCG GGAACAAATG TGTGCACGGC
    ACCTGCTTGC CCATCAACGC CTTCTCCTAC AGCTGTAAGT GTCTGGAGGG CCACGGGGGC
    GTCCTCTGCG ACGAAGAGGA GGACCTGTTC AACCCCTGCC AGGCCATCAG GTGCAAGCAC
    GGGAAATGCA GGCTCTCGGG CCTGGGCCAG CCCTACTGCG AATGCAGCAG CGGGTACACG
    GGGGATAGCT GCGACCGAGA AGTGTCCTGT CGGGGCGAGC GCGTCCGGGA CTACTACCCA
    AAGCAGCAGG GCTACGCGGC CTGCCAGACC ACCAAGAAGG TGTCGCGGCT GGAGTGCAGG
    GGCGGCTGCG CGGCCGGGCA GTGCTGCGGG CCGCTGCGGA GCAAGCGGCG GAAATACTCC
    TTCGAGTGCA CGGACGGCTC GTCGTTCGTG GACGAGGTGG AGAAGGTGGT CAAGTGCGGC
    TGCAGCAGGT GCGCCGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35887
    Clone ID Related Accession (Same CDS sequence) XM_014112673.1 , XM_014112673.2
    Accession Version XM_014112673.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4581bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product slit homolog 2 protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112673.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
    GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC
    TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG
    GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC
    CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG
    GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG
    TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG
    GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC
    AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC
    ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT
    TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG
    GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT
    TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG
    AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT
    CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC
    TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT
    GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC
    AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC
    TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC
    CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG
    TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG GTACAGAAGA TTATCGATCA AAATTAAGCG GGGACTGCTT TGCAGATCTG
    GCTTGCCCTG AAAAGTGCCG CTGTGAAGGA ACCACAGTAG ATTGCTCCAA TCAAAAACTC
    ACCAAAATCC CAGACCACAT CCCCCAGTAC ACTGCAGAGC TGCGTCTCAA TAATAATGAA
    TTCACAGTGC TGGAAGCTAC AGGAATCTTC AAGAAACTTC CGCAGTTACG TAAAATAAAC
    TTCAGCAACA ACAAGATCAC AGACATTGAA GAAGGAGCAT TTGAAGGAGC AGCTGGTGTA
    AACGAAATCC TTCTCACGAG TAACCGTTTG GAAAATGTTC AGCATAAGAT GTTCAAGGGA
    TTGGAAAGCC TGAAAACGTT GATGTTGCGA AGCAATCGCA TAAGCTGCGT TGGCAACGAT
    AGCTTCATAG GCCTGAGCTC TGTGCGGTTG CTTTCGCTGT ACGATAATCA GATCGCCACC
    ATCGCGCCGG GGGCGTTCGA CACCCTGCAC TCGTTGTCCA CCCTAAACCT GTTGGCCAAC
    CCTTTTAACT GCAACTGCTA CCTGGCTTGG CTGGGCGAGT GGCTCAGGAA GAAAAGAATT
    GTAACCGGAA ATCCTCGCTG TCAAAAACCA TACTTCCTCA AAGAAATCCC CATCCAGGAC
    GTCGCCATTC AAGACTTCAC GTGTGACGAC GGAAATGACG ACAGTAGCTG TTCTCCACTC
    TCGCGCTGTC CCACGGAATG CACGTGCTTG GATACAGTTG TCCGATGTAG CAACAAGGGC
    CTGAAGGTCT TGCCCAAAGG TATTCCCAGA GACGTCACTG AACTGTATCT GGATGGGAAC
    CACTTTACCT TGGTTCCCAA GGAGCTCTAT AACTACAAAC ATCTAACGCT TATAGACCTG
    AGCAACAACC GCATAAGCAC TCTTTCTAAT CAGAGCTTCA GCAACATGAC CCAGCTCCTC
    ACCCTAATTC TCAGTTACAA CCGTTTGAGA TGTATTCCTC CTCGAACCTT CGATGGACTC
    AAGTCTCTCC GATTACTTTC ATTACATGGA AATGACATTT CTGTTGTGCC TGAAGGTGCT
    TTCAGTGATC TCTCTGCATT ATCACACCTA GCAATCGGAG CCAACCCCCT TTACTGTGAT
    TGCAACATGC AGTGGTTATC GGACTGGGTA AAGTCGGAAT ACAAAGAACC CGGGATTGCT
    CGCTGTGCCG GCCCCGGAGA AATGGCAGAT AAATTATTAC TCACGACTCC CTCCAAAAAA
    TTTACATGTC AAGGTCCTGT GGATATCAAT ATTCTAGCTA AATGTAATCC CTGCTTATCA
    AACCCATGTA AGAATGATGG CACCTGTAAC AATGATCCAG TCGACTTTTA TCGCTGTACC
    TGTCCGTATG GTTTCAAGGG GCAGGACTGT GATGTCCCAA TCCACGCATG CATCAGTAAC
    CCGTGTACAC ATGGAGGAAC TTGCCACTTA AAGGAGGGAG AAAAAGATGG ATTCTGGTGT
    ATTTGTGCCG ATGGATTTGA AGGAGAAAAT TGTGAAGTCA ATGTTGATGA CTGTGAAGAT
    AATGACTGTG AAAATAACTC TACGTGTGTC GATGGAATTA ATAACTACAC ATGCCTTTGT
    CCGCCTGAGT ACACAGGCGA GTTGTGTGAG GAGAAGCTGG ACTTCTGCGC TCAGGACCTG
    AACCCCTGCC AGCACGACTC CAAGTGCATC CTGATGCCCA AAGGATTCAA ATGCGACTGC
    ACGCCGGGGT ACGTGGGCGA GCACTGCGAC ATCGACTTCG ACGACTGCCA GGATCACAAG
    TGTAAAAACG GAGCGCACTG CACGGACGCG GTGAACGGCT ACACGTGCAC CTGCCCCGAA
    GGCTACAGCG GCTTGTTCTG TGAATTCTCC CCGCCCATGG TCCTCCCACG CACCAGCCCC
    TGTGACAACT TCGACTGTCA GAACGGGGCG CAGTGCATCG TCAGGGCGGG CGAGCCAATC
    TGCCAGTGTC TGCCCGGCTA CCAGGGGGAC AAGTGTGAGA AGTTGGTCAG CGTGAACTTC
    GTGAACAAAG AGTCGTATCT TCAAATTCCT TCAGCCAAGG TCCGGCCCCA AACGAACATC
    ACCCTGCAGA TTGCCACCGA CGAAGACAGC GGGATCCTCC TGTACAAGGG CGACAAGGAC
    CACATTGCCG TGGAGCTGTA TCGGGGACGG GTGCGCGCCA GCTACGACAC CGGCTCGCAC
    CCCGCTTCTG CCATTTACAG CGTGGAGACG ATCAATGATG GAAACTTTCA CATTGTGGAA
    CTACTTGCCC TGGATCAGAG CCTGTCCCTC TCCGTGGATG GAGGGAGCCC CAAAATCATC
    ACCAACTTGT CAAAGCAGTC CACTCTGAAT TTTGACTCTC CACTCTATGT AGGAGGCATG
    CCCGGGAGGA ACAACGTGGC CGCGGCCCTG CGCCAGGCCC CGGGGCACAA CGGCACCAGC
    TTCCACGGCT GCATCCGGAA CCTGTATATC AACAGCGAGC TCCAGGACTT CCGCCAGGTG
    CCCATGCAGA CCGGCATCCT GCCCGGCTGC GAGCCGTGCC ACAGGAAGGT GTGTGCCCAC
    GGCGCGTGCC AGCCCAGCAG CCAGTCGGGC TTCACCTGCG AGTGCGAGGA GGGCTGGACG
    GGGCCCCTGT GTGACCAGAG GACCAACGAC CCCTGTCTCG GGAACAAATG TGTGCACGGC
    ACCTGCTTGC CCATCAACGC CTTCTCCTAC AGCTGTAAGT GTCTGGAGGG CCACGGGGGC
    GTCCTCTGCG ACGAAGAGGA GGACCTGTTC AACCCCTGCC AGGCCATCAG GTGCAAGCAC
    GGGAAATGCA GGCTCTCGGG CCTGGGCCAG CCCTACTGCG AATGCAGCAG CGGGTACACG
    GGGGATAGCT GCGACCGAGA AGTGTCCTGT CGGGGCGAGC GCGTCCGGGA CTACTACCCA
    AAGCAGCAGG GCTACGCGGC CTGCCAGACC ACCAAGAAGG TGTCGCGGCT GGAGTGCAGG
    GGCGGCTGCG CGGCCGGGCA GTGCTGCGGG CCGCTGCGGA GCAAGCGGCG GAAATACTCC
    TTCGAGTGCA CGGACGGCTC GTCGTTCGTG GACGAGGTGG AGAAGGTGGT CAAGTGCGGC
    TGCAGCAGGT GCGCCGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968148.1
    CDS212..4792
    Translation

    Target ORF information:

    RefSeq Version XM_014112673.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112673.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
    GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC
    TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG
    GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC
    CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG
    GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG
    TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG
    GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC
    AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC
    ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT
    TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG
    GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT
    TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG
    AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT
    CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC
    TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT
    GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC
    AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC
    TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC
    CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG
    TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG GTACAGAAGA TTATCGATCA AAATTAAGCG GGGACTGCTT TGCAGATCTG
    GCTTGCCCTG AAAAGTGCCG CTGTGAAGGA ACCACAGTAG ATTGCTCCAA TCAAAAACTC
    ACCAAAATCC CAGACCACAT CCCCCAGTAC ACTGCAGAGC TGCGTCTCAA TAATAATGAA
    TTCACAGTGC TGGAAGCTAC AGGAATCTTC AAGAAACTTC CGCAGTTACG TAAAATAAAC
    TTCAGCAACA ACAAGATCAC AGACATTGAA GAAGGAGCAT TTGAAGGAGC AGCTGGTGTA
    AACGAAATCC TTCTCACGAG TAACCGTTTG GAAAATGTTC AGCATAAGAT GTTCAAGGGA
    TTGGAAAGCC TGAAAACGTT GATGTTGCGA AGCAATCGCA TAAGCTGCGT TGGCAACGAT
    AGCTTCATAG GCCTGAGCTC TGTGCGGTTG CTTTCGCTGT ACGATAATCA GATCGCCACC
    ATCGCGCCGG GGGCGTTCGA CACCCTGCAC TCGTTGTCCA CCCTAAACCT GTTGGCCAAC
    CCTTTTAACT GCAACTGCTA CCTGGCTTGG CTGGGCGAGT GGCTCAGGAA GAAAAGAATT
    GTAACCGGAA ATCCTCGCTG TCAAAAACCA TACTTCCTCA AAGAAATCCC CATCCAGGAC
    GTCGCCATTC AAGACTTCAC GTGTGACGAC GGAAATGACG ACAGTAGCTG TTCTCCACTC
    TCGCGCTGTC CCACGGAATG CACGTGCTTG GATACAGTTG TCCGATGTAG CAACAAGGGC
    CTGAAGGTCT TGCCCAAAGG TATTCCCAGA GACGTCACTG AACTGTATCT GGATGGGAAC
    CACTTTACCT TGGTTCCCAA GGAGCTCTAT AACTACAAAC ATCTAACGCT TATAGACCTG
    AGCAACAACC GCATAAGCAC TCTTTCTAAT CAGAGCTTCA GCAACATGAC CCAGCTCCTC
    ACCCTAATTC TCAGTTACAA CCGTTTGAGA TGTATTCCTC CTCGAACCTT CGATGGACTC
    AAGTCTCTCC GATTACTTTC ATTACATGGA AATGACATTT CTGTTGTGCC TGAAGGTGCT
    TTCAGTGATC TCTCTGCATT ATCACACCTA GCAATCGGAG CCAACCCCCT TTACTGTGAT
    TGCAACATGC AGTGGTTATC GGACTGGGTA AAGTCGGAAT ACAAAGAACC CGGGATTGCT
    CGCTGTGCCG GCCCCGGAGA AATGGCAGAT AAATTATTAC TCACGACTCC CTCCAAAAAA
    TTTACATGTC AAGGTCCTGT GGATATCAAT ATTCTAGCTA AATGTAATCC CTGCTTATCA
    AACCCATGTA AGAATGATGG CACCTGTAAC AATGATCCAG TCGACTTTTA TCGCTGTACC
    TGTCCGTATG GTTTCAAGGG GCAGGACTGT GATGTCCCAA TCCACGCATG CATCAGTAAC
    CCGTGTACAC ATGGAGGAAC TTGCCACTTA AAGGAGGGAG AAAAAGATGG ATTCTGGTGT
    ATTTGTGCCG ATGGATTTGA AGGAGAAAAT TGTGAAGTCA ATGTTGATGA CTGTGAAGAT
    AATGACTGTG AAAATAACTC TACGTGTGTC GATGGAATTA ATAACTACAC ATGCCTTTGT
    CCGCCTGAGT ACACAGGCGA GTTGTGTGAG GAGAAGCTGG ACTTCTGCGC TCAGGACCTG
    AACCCCTGCC AGCACGACTC CAAGTGCATC CTGATGCCCA AAGGATTCAA ATGCGACTGC
    ACGCCGGGGT ACGTGGGCGA GCACTGCGAC ATCGACTTCG ACGACTGCCA GGATCACAAG
    TGTAAAAACG GAGCGCACTG CACGGACGCG GTGAACGGCT ACACGTGCAC CTGCCCCGAA
    GGCTACAGCG GCTTGTTCTG TGAATTCTCC CCGCCCATGG TCCTCCCACG CACCAGCCCC
    TGTGACAACT TCGACTGTCA GAACGGGGCG CAGTGCATCG TCAGGGCGGG CGAGCCAATC
    TGCCAGTGTC TGCCCGGCTA CCAGGGGGAC AAGTGTGAGA AGTTGGTCAG CGTGAACTTC
    GTGAACAAAG AGTCGTATCT TCAAATTCCT TCAGCCAAGG TCCGGCCCCA AACGAACATC
    ACCCTGCAGA TTGCCACCGA CGAAGACAGC GGGATCCTCC TGTACAAGGG CGACAAGGAC
    CACATTGCCG TGGAGCTGTA TCGGGGACGG GTGCGCGCCA GCTACGACAC CGGCTCGCAC
    CCCGCTTCTG CCATTTACAG CGTGGAGACG ATCAATGATG GAAACTTTCA CATTGTGGAA
    CTACTTGCCC TGGATCAGAG CCTGTCCCTC TCCGTGGATG GAGGGAGCCC CAAAATCATC
    ACCAACTTGT CAAAGCAGTC CACTCTGAAT TTTGACTCTC CACTCTATGT AGGAGGCATG
    CCCGGGAGGA ACAACGTGGC CGCGGCCCTG CGCCAGGCCC CGGGGCACAA CGGCACCAGC
    TTCCACGGCT GCATCCGGAA CCTGTATATC AACAGCGAGC TCCAGGACTT CCGCCAGGTG
    CCCATGCAGA CCGGCATCCT GCCCGGCTGC GAGCCGTGCC ACAGGAAGGT GTGTGCCCAC
    GGCGCGTGCC AGCCCAGCAG CCAGTCGGGC TTCACCTGCG AGTGCGAGGA GGGCTGGACG
    GGGCCCCTGT GTGACCAGAG GACCAACGAC CCCTGTCTCG GGAACAAATG TGTGCACGGC
    ACCTGCTTGC CCATCAACGC CTTCTCCTAC AGCTGTAAGT GTCTGGAGGG CCACGGGGGC
    GTCCTCTGCG ACGAAGAGGA GGACCTGTTC AACCCCTGCC AGGCCATCAG GTGCAAGCAC
    GGGAAATGCA GGCTCTCGGG CCTGGGCCAG CCCTACTGCG AATGCAGCAG CGGGTACACG
    GGGGATAGCT GCGACCGAGA AGTGTCCTGT CGGGGCGAGC GCGTCCGGGA CTACTACCCA
    AAGCAGCAGG GCTACGCGGC CTGCCAGACC ACCAAGAAGG TGTCGCGGCT GGAGTGCAGG
    GGCGGCTGCG CGGCCGGGCA GTGCTGCGGG CCGCTGCGGA GCAAGCGGCG GAAATACTCC
    TTCGAGTGCA CGGACGGCTC GTCGTTCGTG GACGAGGTGG AGAAGGTGGT CAAGTGCGGC
    TGCAGCAGGT GCGCCGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35888
    Clone ID Related Accession (Same CDS sequence) XM_014112674.1 , XM_014112674.2
    Accession Version XM_014112674.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4569bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product slit homolog 2 protein isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
    GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC
    TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG
    GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC
    CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG
    GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG
    TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG
    GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC
    AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC
    ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT
    TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGGCCAC
    CAGTCCTTCA TGGCTCCTTC TTGCAGCGTC CTGCATTGTC CAGCCGCTTG TACCTGTAGC
    AACAATATCG TAGACTGTCG TGGGAAAGGT CTCACTGAGA TCCCCACGAA CCTGCCAGAG
    ACCATCACAG AAATACGTTT GGAACAGAAC TCAATCAAGG TCATCCCTCC TGGAGCTTTC
    TCACCATATA AAAAGCTTAG AAGAATTGAC CTGAGCAATA ATCAGATCTC TGAACTAGCA
    CCGGACGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC
    ACGGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTGCT ATTATTGAAT
    GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ATCTGCACAA CCTGAATCTT
    CTCTCCCTGT ACGACAACAA GCTGCAGACC ATCGCCAAGG GGACCTTCTC ACCTCTCCGG
    GCCATTCAGA CCATGCACCT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG
    CTGGCGGACT ATCTCCACAC CAACCCCATC GAGACCAGTG GTGCCCGGTG CACCAGCCCC
    CGGCGCCTGG CAAACAAAAG AATCGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGGT
    ACAGAAGATT ATCGATCAAA ATTAAGCGGG GACTGCTTTG CAGATCTGGC TTGCCCTGAA
    AAGTGCCGCT GTGAAGGAAC CACAGTAGAT TGCTCCAATC AAAAACTCAC CAAAATCCCA
    GACCACATCC CCCAGTACAC TGCAGAGCTG CGTCTCAATA ATAATGAATT CACAGTGCTG
    GAAGCTACAG GAATCTTCAA GAAACTTCCG CAGTTACGTA AAATAAACTT CAGCAACAAC
    AAGATCACAG ACATTGAAGA AGGAGCATTT GAAGGAGCAG CTGGTGTAAA CGAAATCCTT
    CTCACGAGTA ACCGTTTGGA AAATGTTCAG CATAAGATGT TCAAGGGATT GGAAAGCCTG
    AAAACGTTGA TGTTGCGAAG CAATCGCATA AGCTGCGTTG GCAACGATAG CTTCATAGGC
    CTGAGCTCTG TGCGGTTGCT TTCGCTGTAC GATAATCAGA TCGCCACCAT CGCGCCGGGG
    GCGTTCGACA CCCTGCACTC GTTGTCCACC CTAAACCTGT TGGCCAACCC TTTTAACTGC
    AACTGCTACC TGGCTTGGCT GGGCGAGTGG CTCAGGAAGA AAAGAATTGT AACCGGAAAT
    CCTCGCTGTC AAAAACCATA CTTCCTCAAA GAAATCCCCA TCCAGGACGT CGCCATTCAA
    GACTTCACGT GTGACGACGG AAATGACGAC AGTAGCTGTT CTCCACTCTC GCGCTGTCCC
    ACGGAATGCA CGTGCTTGGA TACAGTTGTC CGATGTAGCA ACAAGGGCCT GAAGGTCTTG
    CCCAAAGGTA TTCCCAGAGA CGTCACTGAA CTGTATCTGG ATGGGAACCA CTTTACCTTG
    GTTCCCAAGG AGCTCTATAA CTACAAACAT CTAACGCTTA TAGACCTGAG CAACAACCGC
    ATAAGCACTC TTTCTAATCA GAGCTTCAGC AACATGACCC AGCTCCTCAC CCTAATTCTC
    AGTTACAACC GTTTGAGATG TATTCCTCCT CGAACCTTCG ATGGACTCAA GTCTCTCCGA
    TTACTTTCAT TACATGGAAA TGACATTTCT GTTGTGCCTG AAGGTGCTTT CAGTGATCTC
    TCTGCATTAT CACACCTAGC AATCGGAGCC AACCCCCTTT ACTGTGATTG CAACATGCAG
    TGGTTATCGG ACTGGGTAAA GTCGGAATAC AAAGAACCCG GGATTGCTCG CTGTGCCGGC
    CCCGGAGAAA TGGCAGATAA ATTATTACTC ACGACTCCCT CCAAAAAATT TACATGTCAA
    GGTCCTGTGG ATATCAATAT TCTAGCTAAA TGTAATCCCT GCTTATCAAA CCCATGTAAG
    AATGATGGCA CCTGTAACAA TGATCCAGTC GACTTTTATC GCTGTACCTG TCCGTATGGT
    TTCAAGGGGC AGGACTGTGA TGTCCCAATC CACGCATGCA TCAGTAACCC GTGTACACAT
    GGAGGAACTT GCCACTTAAA GGAGGGAGAA AAAGATGGAT TCTGGTGTAT TTGTGCCGAT
    GGATTTGAAG GAGAAAATTG TGAAGTCAAT GTTGATGACT GTGAAGATAA TGACTGTGAA
    AATAACTCTA CGTGTGTCGA TGGAATTAAT AACTACACAT GCCTTTGTCC GCCTGAGTAC
    ACAGGCGAGT TGTGTGAGGA GAAGCTGGAC TTCTGCGCTC AGGACCTGAA CCCCTGCCAG
    CACGACTCCA AGTGCATCCT GATGCCCAAA GGATTCAAAT GCGACTGCAC GCCGGGGTAC
    GTGGGCGAGC ACTGCGACAT CGACTTCGAC GACTGCCAGG ATCACAAGTG TAAAAACGGA
    GCGCACTGCA CGGACGCGGT GAACGGCTAC ACGTGCACCT GCCCCGAAGG CTACAGCGGC
    TTGTTCTGTG AATTCTCCCC GCCCATGGTC CTCCCACGCA CCAGCCCCTG TGACAACTTC
    GACTGTCAGA ACGGGGCGCA GTGCATCGTC AGGGCGGGCG AGCCAATCTG CCAGTGTCTG
    CCCGGCTACC AGGGGGACAA GTGTGAGAAG TTGGTCAGCG TGAACTTCGT GAACAAAGAG
    TCGTATCTTC AAATTCCTTC AGCCAAGGTC CGGCCCCAAA CGAACATCAC CCTGCAGATT
    GCCACCGACG AAGACAGCGG GATCCTCCTG TACAAGGGCG ACAAGGACCA CATTGCCGTG
    GAGCTGTATC GGGGACGGGT GCGCGCCAGC TACGACACCG GCTCGCACCC CGCTTCTGCC
    ATTTACAGCG TGGAGACGAT CAATGATGGA AACTTTCACA TTGTGGAACT ACTTGCCCTG
    GATCAGAGCC TGTCCCTCTC CGTGGATGGA GGGAGCCCCA AAATCATCAC CAACTTGTCA
    AAGCAGTCCA CTCTGAATTT TGACTCTCCA CTCTATGTAG GAGGCATGCC CGGGAGGAAC
    AACGTGGCCG CGGCCCTGCG CCAGGCCCCG GGGCACAACG GCACCAGCTT CCACGGCTGC
    ATCCGGAACC TGTATATCAA CAGCGAGCTC CAGGACTTCC GCCAGGTGCC CATGCAGACC
    GGCATCCTGC CCGGCTGCGA GCCGTGCCAC AGGAAGGTGT GTGCCCACGG CGCGTGCCAG
    CCCAGCAGCC AGTCGGGCTT CACCTGCGAG TGCGAGGAGG GCTGGACGGG GCCCCTGTGT
    GACCAGAGGA CCAACGACCC CTGTCTCGGG AACAAATGTG TGCACGGCAC CTGCTTGCCC
    ATCAACGCCT TCTCCTACAG CTGTAAGTGT CTGGAGGGCC ACGGGGGCGT CCTCTGCGAC
    GAAGAGGAGG ACCTGTTCAA CCCCTGCCAG GCCATCAGGT GCAAGCACGG GAAATGCAGG
    CTCTCGGGCC TGGGCCAGCC CTACTGCGAA TGCAGCAGCG GGTACACGGG GGATAGCTGC
    GACCGAGAAG TGTCCTGTCG GGGCGAGCGC GTCCGGGACT ACTACCCAAA GCAGCAGGGC
    TACGCGGCCT GCCAGACCAC CAAGAAGGTG TCGCGGCTGG AGTGCAGGGG CGGCTGCGCG
    GCCGGGCAGT GCTGCGGGCC GCTGCGGAGC AAGCGGCGGA AATACTCCTT CGAGTGCACG
    GACGGCTCGT CGTTCGTGGA CGAGGTGGAG AAGGTGGTCA AGTGCGGCTG CAGCAGGTGC
    GCCGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968149.1
    CDS86..4654
    Translation

    Target ORF information:

    RefSeq Version XM_014112674.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112674.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
    GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC
    TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG
    GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC
    CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG
    GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG
    TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG
    GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC
    AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC
    ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT
    TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGGCCAC
    CAGTCCTTCA TGGCTCCTTC TTGCAGCGTC CTGCATTGTC CAGCCGCTTG TACCTGTAGC
    AACAATATCG TAGACTGTCG TGGGAAAGGT CTCACTGAGA TCCCCACGAA CCTGCCAGAG
    ACCATCACAG AAATACGTTT GGAACAGAAC TCAATCAAGG TCATCCCTCC TGGAGCTTTC
    TCACCATATA AAAAGCTTAG AAGAATTGAC CTGAGCAATA ATCAGATCTC TGAACTAGCA
    CCGGACGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC
    ACGGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTGCT ATTATTGAAT
    GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ATCTGCACAA CCTGAATCTT
    CTCTCCCTGT ACGACAACAA GCTGCAGACC ATCGCCAAGG GGACCTTCTC ACCTCTCCGG
    GCCATTCAGA CCATGCACCT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG
    CTGGCGGACT ATCTCCACAC CAACCCCATC GAGACCAGTG GTGCCCGGTG CACCAGCCCC
    CGGCGCCTGG CAAACAAAAG AATCGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGGT
    ACAGAAGATT ATCGATCAAA ATTAAGCGGG GACTGCTTTG CAGATCTGGC TTGCCCTGAA
    AAGTGCCGCT GTGAAGGAAC CACAGTAGAT TGCTCCAATC AAAAACTCAC CAAAATCCCA
    GACCACATCC CCCAGTACAC TGCAGAGCTG CGTCTCAATA ATAATGAATT CACAGTGCTG
    GAAGCTACAG GAATCTTCAA GAAACTTCCG CAGTTACGTA AAATAAACTT CAGCAACAAC
    AAGATCACAG ACATTGAAGA AGGAGCATTT GAAGGAGCAG CTGGTGTAAA CGAAATCCTT
    CTCACGAGTA ACCGTTTGGA AAATGTTCAG CATAAGATGT TCAAGGGATT GGAAAGCCTG
    AAAACGTTGA TGTTGCGAAG CAATCGCATA AGCTGCGTTG GCAACGATAG CTTCATAGGC
    CTGAGCTCTG TGCGGTTGCT TTCGCTGTAC GATAATCAGA TCGCCACCAT CGCGCCGGGG
    GCGTTCGACA CCCTGCACTC GTTGTCCACC CTAAACCTGT TGGCCAACCC TTTTAACTGC
    AACTGCTACC TGGCTTGGCT GGGCGAGTGG CTCAGGAAGA AAAGAATTGT AACCGGAAAT
    CCTCGCTGTC AAAAACCATA CTTCCTCAAA GAAATCCCCA TCCAGGACGT CGCCATTCAA
    GACTTCACGT GTGACGACGG AAATGACGAC AGTAGCTGTT CTCCACTCTC GCGCTGTCCC
    ACGGAATGCA CGTGCTTGGA TACAGTTGTC CGATGTAGCA ACAAGGGCCT GAAGGTCTTG
    CCCAAAGGTA TTCCCAGAGA CGTCACTGAA CTGTATCTGG ATGGGAACCA CTTTACCTTG
    GTTCCCAAGG AGCTCTATAA CTACAAACAT CTAACGCTTA TAGACCTGAG CAACAACCGC
    ATAAGCACTC TTTCTAATCA GAGCTTCAGC AACATGACCC AGCTCCTCAC CCTAATTCTC
    AGTTACAACC GTTTGAGATG TATTCCTCCT CGAACCTTCG ATGGACTCAA GTCTCTCCGA
    TTACTTTCAT TACATGGAAA TGACATTTCT GTTGTGCCTG AAGGTGCTTT CAGTGATCTC
    TCTGCATTAT CACACCTAGC AATCGGAGCC AACCCCCTTT ACTGTGATTG CAACATGCAG
    TGGTTATCGG ACTGGGTAAA GTCGGAATAC AAAGAACCCG GGATTGCTCG CTGTGCCGGC
    CCCGGAGAAA TGGCAGATAA ATTATTACTC ACGACTCCCT CCAAAAAATT TACATGTCAA
    GGTCCTGTGG ATATCAATAT TCTAGCTAAA TGTAATCCCT GCTTATCAAA CCCATGTAAG
    AATGATGGCA CCTGTAACAA TGATCCAGTC GACTTTTATC GCTGTACCTG TCCGTATGGT
    TTCAAGGGGC AGGACTGTGA TGTCCCAATC CACGCATGCA TCAGTAACCC GTGTACACAT
    GGAGGAACTT GCCACTTAAA GGAGGGAGAA AAAGATGGAT TCTGGTGTAT TTGTGCCGAT
    GGATTTGAAG GAGAAAATTG TGAAGTCAAT GTTGATGACT GTGAAGATAA TGACTGTGAA
    AATAACTCTA CGTGTGTCGA TGGAATTAAT AACTACACAT GCCTTTGTCC GCCTGAGTAC
    ACAGGCGAGT TGTGTGAGGA GAAGCTGGAC TTCTGCGCTC AGGACCTGAA CCCCTGCCAG
    CACGACTCCA AGTGCATCCT GATGCCCAAA GGATTCAAAT GCGACTGCAC GCCGGGGTAC
    GTGGGCGAGC ACTGCGACAT CGACTTCGAC GACTGCCAGG ATCACAAGTG TAAAAACGGA
    GCGCACTGCA CGGACGCGGT GAACGGCTAC ACGTGCACCT GCCCCGAAGG CTACAGCGGC
    TTGTTCTGTG AATTCTCCCC GCCCATGGTC CTCCCACGCA CCAGCCCCTG TGACAACTTC
    GACTGTCAGA ACGGGGCGCA GTGCATCGTC AGGGCGGGCG AGCCAATCTG CCAGTGTCTG
    CCCGGCTACC AGGGGGACAA GTGTGAGAAG TTGGTCAGCG TGAACTTCGT GAACAAAGAG
    TCGTATCTTC AAATTCCTTC AGCCAAGGTC CGGCCCCAAA CGAACATCAC CCTGCAGATT
    GCCACCGACG AAGACAGCGG GATCCTCCTG TACAAGGGCG ACAAGGACCA CATTGCCGTG
    GAGCTGTATC GGGGACGGGT GCGCGCCAGC TACGACACCG GCTCGCACCC CGCTTCTGCC
    ATTTACAGCG TGGAGACGAT CAATGATGGA AACTTTCACA TTGTGGAACT ACTTGCCCTG
    GATCAGAGCC TGTCCCTCTC CGTGGATGGA GGGAGCCCCA AAATCATCAC CAACTTGTCA
    AAGCAGTCCA CTCTGAATTT TGACTCTCCA CTCTATGTAG GAGGCATGCC CGGGAGGAAC
    AACGTGGCCG CGGCCCTGCG CCAGGCCCCG GGGCACAACG GCACCAGCTT CCACGGCTGC
    ATCCGGAACC TGTATATCAA CAGCGAGCTC CAGGACTTCC GCCAGGTGCC CATGCAGACC
    GGCATCCTGC CCGGCTGCGA GCCGTGCCAC AGGAAGGTGT GTGCCCACGG CGCGTGCCAG
    CCCAGCAGCC AGTCGGGCTT CACCTGCGAG TGCGAGGAGG GCTGGACGGG GCCCCTGTGT
    GACCAGAGGA CCAACGACCC CTGTCTCGGG AACAAATGTG TGCACGGCAC CTGCTTGCCC
    ATCAACGCCT TCTCCTACAG CTGTAAGTGT CTGGAGGGCC ACGGGGGCGT CCTCTGCGAC
    GAAGAGGAGG ACCTGTTCAA CCCCTGCCAG GCCATCAGGT GCAAGCACGG GAAATGCAGG
    CTCTCGGGCC TGGGCCAGCC CTACTGCGAA TGCAGCAGCG GGTACACGGG GGATAGCTGC
    GACCGAGAAG TGTCCTGTCG GGGCGAGCGC GTCCGGGACT ACTACCCAAA GCAGCAGGGC
    TACGCGGCCT GCCAGACCAC CAAGAAGGTG TCGCGGCTGG AGTGCAGGGG CGGCTGCGCG
    GCCGGGCAGT GCTGCGGGCC GCTGCGGAGC AAGCGGCGGA AATACTCCTT CGAGTGCACG
    GACGGCTCGT CGTTCGTGGA CGAGGTGGAG AAGGTGGTCA AGTGCGGCTG CAGCAGGTGC
    GCCGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35888
    Clone ID Related Accession (Same CDS sequence) XM_014112674.1 , XM_014112674.2
    Accession Version XM_014112674.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4569bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product slit homolog 2 protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112674.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
    GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC
    TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG
    GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC
    CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG
    GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG
    TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG
    GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC
    AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC
    ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT
    TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGGCCAC
    CAGTCCTTCA TGGCTCCTTC TTGCAGCGTC CTGCATTGTC CAGCCGCTTG TACCTGTAGC
    AACAATATCG TAGACTGTCG TGGGAAAGGT CTCACTGAGA TCCCCACGAA CCTGCCAGAG
    ACCATCACAG AAATACGTTT GGAACAGAAC TCAATCAAGG TCATCCCTCC TGGAGCTTTC
    TCACCATATA AAAAGCTTAG AAGAATTGAC CTGAGCAATA ATCAGATCTC TGAACTAGCA
    CCGGACGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC
    ACGGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTGCT ATTATTGAAT
    GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ATCTGCACAA CCTGAATCTT
    CTCTCCCTGT ACGACAACAA GCTGCAGACC ATCGCCAAGG GGACCTTCTC ACCTCTCCGG
    GCCATTCAGA CCATGCACCT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG
    CTGGCGGACT ATCTCCACAC CAACCCCATC GAGACCAGTG GTGCCCGGTG CACCAGCCCC
    CGGCGCCTGG CAAACAAAAG AATCGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGGT
    ACAGAAGATT ATCGATCAAA ATTAAGCGGG GACTGCTTTG CAGATCTGGC TTGCCCTGAA
    AAGTGCCGCT GTGAAGGAAC CACAGTAGAT TGCTCCAATC AAAAACTCAC CAAAATCCCA
    GACCACATCC CCCAGTACAC TGCAGAGCTG CGTCTCAATA ATAATGAATT CACAGTGCTG
    GAAGCTACAG GAATCTTCAA GAAACTTCCG CAGTTACGTA AAATAAACTT CAGCAACAAC
    AAGATCACAG ACATTGAAGA AGGAGCATTT GAAGGAGCAG CTGGTGTAAA CGAAATCCTT
    CTCACGAGTA ACCGTTTGGA AAATGTTCAG CATAAGATGT TCAAGGGATT GGAAAGCCTG
    AAAACGTTGA TGTTGCGAAG CAATCGCATA AGCTGCGTTG GCAACGATAG CTTCATAGGC
    CTGAGCTCTG TGCGGTTGCT TTCGCTGTAC GATAATCAGA TCGCCACCAT CGCGCCGGGG
    GCGTTCGACA CCCTGCACTC GTTGTCCACC CTAAACCTGT TGGCCAACCC TTTTAACTGC
    AACTGCTACC TGGCTTGGCT GGGCGAGTGG CTCAGGAAGA AAAGAATTGT AACCGGAAAT
    CCTCGCTGTC AAAAACCATA CTTCCTCAAA GAAATCCCCA TCCAGGACGT CGCCATTCAA
    GACTTCACGT GTGACGACGG AAATGACGAC AGTAGCTGTT CTCCACTCTC GCGCTGTCCC
    ACGGAATGCA CGTGCTTGGA TACAGTTGTC CGATGTAGCA ACAAGGGCCT GAAGGTCTTG
    CCCAAAGGTA TTCCCAGAGA CGTCACTGAA CTGTATCTGG ATGGGAACCA CTTTACCTTG
    GTTCCCAAGG AGCTCTATAA CTACAAACAT CTAACGCTTA TAGACCTGAG CAACAACCGC
    ATAAGCACTC TTTCTAATCA GAGCTTCAGC AACATGACCC AGCTCCTCAC CCTAATTCTC
    AGTTACAACC GTTTGAGATG TATTCCTCCT CGAACCTTCG ATGGACTCAA GTCTCTCCGA
    TTACTTTCAT TACATGGAAA TGACATTTCT GTTGTGCCTG AAGGTGCTTT CAGTGATCTC
    TCTGCATTAT CACACCTAGC AATCGGAGCC AACCCCCTTT ACTGTGATTG CAACATGCAG
    TGGTTATCGG ACTGGGTAAA GTCGGAATAC AAAGAACCCG GGATTGCTCG CTGTGCCGGC
    CCCGGAGAAA TGGCAGATAA ATTATTACTC ACGACTCCCT CCAAAAAATT TACATGTCAA
    GGTCCTGTGG ATATCAATAT TCTAGCTAAA TGTAATCCCT GCTTATCAAA CCCATGTAAG
    AATGATGGCA CCTGTAACAA TGATCCAGTC GACTTTTATC GCTGTACCTG TCCGTATGGT
    TTCAAGGGGC AGGACTGTGA TGTCCCAATC CACGCATGCA TCAGTAACCC GTGTACACAT
    GGAGGAACTT GCCACTTAAA GGAGGGAGAA AAAGATGGAT TCTGGTGTAT TTGTGCCGAT
    GGATTTGAAG GAGAAAATTG TGAAGTCAAT GTTGATGACT GTGAAGATAA TGACTGTGAA
    AATAACTCTA CGTGTGTCGA TGGAATTAAT AACTACACAT GCCTTTGTCC GCCTGAGTAC
    ACAGGCGAGT TGTGTGAGGA GAAGCTGGAC TTCTGCGCTC AGGACCTGAA CCCCTGCCAG
    CACGACTCCA AGTGCATCCT GATGCCCAAA GGATTCAAAT GCGACTGCAC GCCGGGGTAC
    GTGGGCGAGC ACTGCGACAT CGACTTCGAC GACTGCCAGG ATCACAAGTG TAAAAACGGA
    GCGCACTGCA CGGACGCGGT GAACGGCTAC ACGTGCACCT GCCCCGAAGG CTACAGCGGC
    TTGTTCTGTG AATTCTCCCC GCCCATGGTC CTCCCACGCA CCAGCCCCTG TGACAACTTC
    GACTGTCAGA ACGGGGCGCA GTGCATCGTC AGGGCGGGCG AGCCAATCTG CCAGTGTCTG
    CCCGGCTACC AGGGGGACAA GTGTGAGAAG TTGGTCAGCG TGAACTTCGT GAACAAAGAG
    TCGTATCTTC AAATTCCTTC AGCCAAGGTC CGGCCCCAAA CGAACATCAC CCTGCAGATT
    GCCACCGACG AAGACAGCGG GATCCTCCTG TACAAGGGCG ACAAGGACCA CATTGCCGTG
    GAGCTGTATC GGGGACGGGT GCGCGCCAGC TACGACACCG GCTCGCACCC CGCTTCTGCC
    ATTTACAGCG TGGAGACGAT CAATGATGGA AACTTTCACA TTGTGGAACT ACTTGCCCTG
    GATCAGAGCC TGTCCCTCTC CGTGGATGGA GGGAGCCCCA AAATCATCAC CAACTTGTCA
    AAGCAGTCCA CTCTGAATTT TGACTCTCCA CTCTATGTAG GAGGCATGCC CGGGAGGAAC
    AACGTGGCCG CGGCCCTGCG CCAGGCCCCG GGGCACAACG GCACCAGCTT CCACGGCTGC
    ATCCGGAACC TGTATATCAA CAGCGAGCTC CAGGACTTCC GCCAGGTGCC CATGCAGACC
    GGCATCCTGC CCGGCTGCGA GCCGTGCCAC AGGAAGGTGT GTGCCCACGG CGCGTGCCAG
    CCCAGCAGCC AGTCGGGCTT CACCTGCGAG TGCGAGGAGG GCTGGACGGG GCCCCTGTGT
    GACCAGAGGA CCAACGACCC CTGTCTCGGG AACAAATGTG TGCACGGCAC CTGCTTGCCC
    ATCAACGCCT TCTCCTACAG CTGTAAGTGT CTGGAGGGCC ACGGGGGCGT CCTCTGCGAC
    GAAGAGGAGG ACCTGTTCAA CCCCTGCCAG GCCATCAGGT GCAAGCACGG GAAATGCAGG
    CTCTCGGGCC TGGGCCAGCC CTACTGCGAA TGCAGCAGCG GGTACACGGG GGATAGCTGC
    GACCGAGAAG TGTCCTGTCG GGGCGAGCGC GTCCGGGACT ACTACCCAAA GCAGCAGGGC
    TACGCGGCCT GCCAGACCAC CAAGAAGGTG TCGCGGCTGG AGTGCAGGGG CGGCTGCGCG
    GCCGGGCAGT GCTGCGGGCC GCTGCGGAGC AAGCGGCGGA AATACTCCTT CGAGTGCACG
    GACGGCTCGT CGTTCGTGGA CGAGGTGGAG AAGGTGGTCA AGTGCGGCTG CAGCAGGTGC
    GCCGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968149.1
    CDS212..4780
    Translation

    Target ORF information:

    RefSeq Version XM_014112674.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112674.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
    GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC
    TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG
    GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC
    CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG
    GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG
    TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG
    GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC
    AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC
    ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT
    TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGGCCAC
    CAGTCCTTCA TGGCTCCTTC TTGCAGCGTC CTGCATTGTC CAGCCGCTTG TACCTGTAGC
    AACAATATCG TAGACTGTCG TGGGAAAGGT CTCACTGAGA TCCCCACGAA CCTGCCAGAG
    ACCATCACAG AAATACGTTT GGAACAGAAC TCAATCAAGG TCATCCCTCC TGGAGCTTTC
    TCACCATATA AAAAGCTTAG AAGAATTGAC CTGAGCAATA ATCAGATCTC TGAACTAGCA
    CCGGACGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC
    ACGGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTGCT ATTATTGAAT
    GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ATCTGCACAA CCTGAATCTT
    CTCTCCCTGT ACGACAACAA GCTGCAGACC ATCGCCAAGG GGACCTTCTC ACCTCTCCGG
    GCCATTCAGA CCATGCACCT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG
    CTGGCGGACT ATCTCCACAC CAACCCCATC GAGACCAGTG GTGCCCGGTG CACCAGCCCC
    CGGCGCCTGG CAAACAAAAG AATCGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGGT
    ACAGAAGATT ATCGATCAAA ATTAAGCGGG GACTGCTTTG CAGATCTGGC TTGCCCTGAA
    AAGTGCCGCT GTGAAGGAAC CACAGTAGAT TGCTCCAATC AAAAACTCAC CAAAATCCCA
    GACCACATCC CCCAGTACAC TGCAGAGCTG CGTCTCAATA ATAATGAATT CACAGTGCTG
    GAAGCTACAG GAATCTTCAA GAAACTTCCG CAGTTACGTA AAATAAACTT CAGCAACAAC
    AAGATCACAG ACATTGAAGA AGGAGCATTT GAAGGAGCAG CTGGTGTAAA CGAAATCCTT
    CTCACGAGTA ACCGTTTGGA AAATGTTCAG CATAAGATGT TCAAGGGATT GGAAAGCCTG
    AAAACGTTGA TGTTGCGAAG CAATCGCATA AGCTGCGTTG GCAACGATAG CTTCATAGGC
    CTGAGCTCTG TGCGGTTGCT TTCGCTGTAC GATAATCAGA TCGCCACCAT CGCGCCGGGG
    GCGTTCGACA CCCTGCACTC GTTGTCCACC CTAAACCTGT TGGCCAACCC TTTTAACTGC
    AACTGCTACC TGGCTTGGCT GGGCGAGTGG CTCAGGAAGA AAAGAATTGT AACCGGAAAT
    CCTCGCTGTC AAAAACCATA CTTCCTCAAA GAAATCCCCA TCCAGGACGT CGCCATTCAA
    GACTTCACGT GTGACGACGG AAATGACGAC AGTAGCTGTT CTCCACTCTC GCGCTGTCCC
    ACGGAATGCA CGTGCTTGGA TACAGTTGTC CGATGTAGCA ACAAGGGCCT GAAGGTCTTG
    CCCAAAGGTA TTCCCAGAGA CGTCACTGAA CTGTATCTGG ATGGGAACCA CTTTACCTTG
    GTTCCCAAGG AGCTCTATAA CTACAAACAT CTAACGCTTA TAGACCTGAG CAACAACCGC
    ATAAGCACTC TTTCTAATCA GAGCTTCAGC AACATGACCC AGCTCCTCAC CCTAATTCTC
    AGTTACAACC GTTTGAGATG TATTCCTCCT CGAACCTTCG ATGGACTCAA GTCTCTCCGA
    TTACTTTCAT TACATGGAAA TGACATTTCT GTTGTGCCTG AAGGTGCTTT CAGTGATCTC
    TCTGCATTAT CACACCTAGC AATCGGAGCC AACCCCCTTT ACTGTGATTG CAACATGCAG
    TGGTTATCGG ACTGGGTAAA GTCGGAATAC AAAGAACCCG GGATTGCTCG CTGTGCCGGC
    CCCGGAGAAA TGGCAGATAA ATTATTACTC ACGACTCCCT CCAAAAAATT TACATGTCAA
    GGTCCTGTGG ATATCAATAT TCTAGCTAAA TGTAATCCCT GCTTATCAAA CCCATGTAAG
    AATGATGGCA CCTGTAACAA TGATCCAGTC GACTTTTATC GCTGTACCTG TCCGTATGGT
    TTCAAGGGGC AGGACTGTGA TGTCCCAATC CACGCATGCA TCAGTAACCC GTGTACACAT
    GGAGGAACTT GCCACTTAAA GGAGGGAGAA AAAGATGGAT TCTGGTGTAT TTGTGCCGAT
    GGATTTGAAG GAGAAAATTG TGAAGTCAAT GTTGATGACT GTGAAGATAA TGACTGTGAA
    AATAACTCTA CGTGTGTCGA TGGAATTAAT AACTACACAT GCCTTTGTCC GCCTGAGTAC
    ACAGGCGAGT TGTGTGAGGA GAAGCTGGAC TTCTGCGCTC AGGACCTGAA CCCCTGCCAG
    CACGACTCCA AGTGCATCCT GATGCCCAAA GGATTCAAAT GCGACTGCAC GCCGGGGTAC
    GTGGGCGAGC ACTGCGACAT CGACTTCGAC GACTGCCAGG ATCACAAGTG TAAAAACGGA
    GCGCACTGCA CGGACGCGGT GAACGGCTAC ACGTGCACCT GCCCCGAAGG CTACAGCGGC
    TTGTTCTGTG AATTCTCCCC GCCCATGGTC CTCCCACGCA CCAGCCCCTG TGACAACTTC
    GACTGTCAGA ACGGGGCGCA GTGCATCGTC AGGGCGGGCG AGCCAATCTG CCAGTGTCTG
    CCCGGCTACC AGGGGGACAA GTGTGAGAAG TTGGTCAGCG TGAACTTCGT GAACAAAGAG
    TCGTATCTTC AAATTCCTTC AGCCAAGGTC CGGCCCCAAA CGAACATCAC CCTGCAGATT
    GCCACCGACG AAGACAGCGG GATCCTCCTG TACAAGGGCG ACAAGGACCA CATTGCCGTG
    GAGCTGTATC GGGGACGGGT GCGCGCCAGC TACGACACCG GCTCGCACCC CGCTTCTGCC
    ATTTACAGCG TGGAGACGAT CAATGATGGA AACTTTCACA TTGTGGAACT ACTTGCCCTG
    GATCAGAGCC TGTCCCTCTC CGTGGATGGA GGGAGCCCCA AAATCATCAC CAACTTGTCA
    AAGCAGTCCA CTCTGAATTT TGACTCTCCA CTCTATGTAG GAGGCATGCC CGGGAGGAAC
    AACGTGGCCG CGGCCCTGCG CCAGGCCCCG GGGCACAACG GCACCAGCTT CCACGGCTGC
    ATCCGGAACC TGTATATCAA CAGCGAGCTC CAGGACTTCC GCCAGGTGCC CATGCAGACC
    GGCATCCTGC CCGGCTGCGA GCCGTGCCAC AGGAAGGTGT GTGCCCACGG CGCGTGCCAG
    CCCAGCAGCC AGTCGGGCTT CACCTGCGAG TGCGAGGAGG GCTGGACGGG GCCCCTGTGT
    GACCAGAGGA CCAACGACCC CTGTCTCGGG AACAAATGTG TGCACGGCAC CTGCTTGCCC
    ATCAACGCCT TCTCCTACAG CTGTAAGTGT CTGGAGGGCC ACGGGGGCGT CCTCTGCGAC
    GAAGAGGAGG ACCTGTTCAA CCCCTGCCAG GCCATCAGGT GCAAGCACGG GAAATGCAGG
    CTCTCGGGCC TGGGCCAGCC CTACTGCGAA TGCAGCAGCG GGTACACGGG GGATAGCTGC
    GACCGAGAAG TGTCCTGTCG GGGCGAGCGC GTCCGGGACT ACTACCCAAA GCAGCAGGGC
    TACGCGGCCT GCCAGACCAC CAAGAAGGTG TCGCGGCTGG AGTGCAGGGG CGGCTGCGCG
    GCCGGGCAGT GCTGCGGGCC GCTGCGGAGC AAGCGGCGGA AATACTCCTT CGAGTGCACG
    GACGGCTCGT CGTTCGTGGA CGAGGTGGAG AAGGTGGTCA AGTGCGGCTG CAGCAGGTGC
    GCCGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35889
    Clone ID Related Accession (Same CDS sequence) XM_014112675.2 , XM_014112675.1
    Accession Version XM_014112675.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4560bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product slit homolog 2 protein isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112675.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
    GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC
    TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG
    GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC
    CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG
    GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG
    TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG
    GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC
    AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC
    ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT
    TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG
    GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT
    TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG
    AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT
    CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC
    TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT
    GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC
    AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC
    TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC
    CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG
    TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA
    AGCGGGGACT GCTTTGCAGA TCTGGCTTGC CCTGAAAAGT GCCGCTGTGA AGGAACCACA
    GTAGATTGCT CCAATCAAAA ACTCACCAAA ATCCCAGACC ACATCCCCCA GTACACTGCA
    GAGCTGCGTC TCAATAATAA TGAATTCACA GTGCTGGAAG CTACAGGAAT CTTCAAGAAA
    CTTCCGCAGT TACGTAAAAT AAACTTCAGC AACAACAAGA TCACAGACAT TGAAGAAGGA
    GCATTTGAAG GAGCAGCTGG TGTAAACGAA ATCCTTCTCA CGAGTAACCG TTTGGAAAAT
    GTTCAGCATA AGATGTTCAA GGGATTGGAA AGCCTGAAAA CGTTGATGTT GCGAAGCAAT
    CGCATAAGCT GCGTTGGCAA CGATAGCTTC ATAGGCCTGA GCTCTGTGCG GTTGCTTTCG
    CTGTACGATA ATCAGATCGC CACCATCGCG CCGGGGGCGT TCGACACCCT GCACTCGTTG
    TCCACCCTAA ACCTGTTGGC CAACCCTTTT AACTGCAACT GCTACCTGGC TTGGCTGGGC
    GAGTGGCTCA GGAAGAAAAG AATTGTAACC GGAAATCCTC GCTGTCAAAA ACCATACTTC
    CTCAAAGAAA TCCCCATCCA GGACGTCGCC ATTCAAGACT TCACGTGTGA CGACGGAAAT
    GACGACAGTA GCTGTTCTCC ACTCTCGCGC TGTCCCACGG AATGCACGTG CTTGGATACA
    GTTGTCCGAT GTAGCAACAA GGGCCTGAAG GTCTTGCCCA AAGGTATTCC CAGAGACGTC
    ACTGAACTGT ATCTGGATGG GAACCACTTT ACCTTGGTTC CCAAGGAGCT CTATAACTAC
    AAACATCTAA CGCTTATAGA CCTGAGCAAC AACCGCATAA GCACTCTTTC TAATCAGAGC
    TTCAGCAACA TGACCCAGCT CCTCACCCTA ATTCTCAGTT ACAACCGTTT GAGATGTATT
    CCTCCTCGAA CCTTCGATGG ACTCAAGTCT CTCCGATTAC TTTCATTACA TGGAAATGAC
    ATTTCTGTTG TGCCTGAAGG TGCTTTCAGT GATCTCTCTG CATTATCACA CCTAGCAATC
    GGAGCCAACC CCCTTTACTG TGATTGCAAC ATGCAGTGGT TATCGGACTG GGTAAAGTCG
    GAATACAAAG AACCCGGGAT TGCTCGCTGT GCCGGCCCCG GAGAAATGGC AGATAAATTA
    TTACTCACGA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CTGTGGATAT CAATATTCTA
    GCTAAATGTA ATCCCTGCTT ATCAAACCCA TGTAAGAATG ATGGCACCTG TAACAATGAT
    CCAGTCGACT TTTATCGCTG TACCTGTCCG TATGGTTTCA AGGGGCAGGA CTGTGATGTC
    CCAATCCACG CATGCATCAG TAACCCGTGT ACACATGGAG GAACTTGCCA CTTAAAGGAG
    GGAGAAAAAG ATGGATTCTG GTGTATTTGT GCCGATGGAT TTGAAGGAGA AAATTGTGAA
    GTCAATGTTG ATGACTGTGA AGATAATGAC TGTGAAAATA ACTCTACGTG TGTCGATGGA
    ATTAATAACT ACACATGCCT TTGTCCGCCT GAGTACACAG GCGAGTTGTG TGAGGAGAAG
    CTGGACTTCT GCGCTCAGGA CCTGAACCCC TGCCAGCACG ACTCCAAGTG CATCCTGATG
    CCCAAAGGAT TCAAATGCGA CTGCACGCCG GGGTACGTGG GCGAGCACTG CGACATCGAC
    TTCGACGACT GCCAGGATCA CAAGTGTAAA AACGGAGCGC ACTGCACGGA CGCGGTGAAC
    GGCTACACGT GCACCTGCCC CGAAGGCTAC AGCGGCTTGT TCTGTGAATT CTCCCCGCCC
    ATGGTCCTCC CACGCACCAG CCCCTGTGAC AACTTCGACT GTCAGAACGG GGCGCAGTGC
    ATCGTCAGGG CGGGCGAGCC AATCTGCCAG TGTCTGCCCG GCTACCAGGG GGACAAGTGT
    GAGAAGTTGG TCAGCGTGAA CTTCGTGAAC AAAGAGTCGT ATCTTCAAAT TCCTTCAGCC
    AAGGTCCGGC CCCAAACGAA CATCACCCTG CAGATTGCCA CCGACGAAGA CAGCGGGATC
    CTCCTGTACA AGGGCGACAA GGACCACATT GCCGTGGAGC TGTATCGGGG ACGGGTGCGC
    GCCAGCTACG ACACCGGCTC GCACCCCGCT TCTGCCATTT ACAGCGTGGA GACGATCAAT
    GATGGAAACT TTCACATTGT GGAACTACTT GCCCTGGATC AGAGCCTGTC CCTCTCCGTG
    GATGGAGGGA GCCCCAAAAT CATCACCAAC TTGTCAAAGC AGTCCACTCT GAATTTTGAC
    TCTCCACTCT ATGTAGGAGG CATGCCCGGG AGGAACAACG TGGCCGCGGC CCTGCGCCAG
    GCCCCGGGGC ACAACGGCAC CAGCTTCCAC GGCTGCATCC GGAACCTGTA TATCAACAGC
    GAGCTCCAGG ACTTCCGCCA GGTGCCCATG CAGACCGGCA TCCTGCCCGG CTGCGAGCCG
    TGCCACAGGA AGGTGTGTGC CCACGGCGCG TGCCAGCCCA GCAGCCAGTC GGGCTTCACC
    TGCGAGTGCG AGGAGGGCTG GACGGGGCCC CTGTGTGACC AGAGGACCAA CGACCCCTGT
    CTCGGGAACA ACTGTAAGTG TCTGGAGGGC CACGGGGGCG TCCTCTGCGA CGAAGAGGAG
    GACCTGTTCA ACCCCTGCCA GGCCATCAGG TGCAAGCACG GGAAATGCAG GCTCTCGGGC
    CTGGGCCAGC CCTACTGCGA ATGCAGCAGC GGGTACACGG GGGATAGCTG CGACCGAGAA
    GTGTCCTGTC GGGGCGAGCG CGTCCGGGAC TACTACCCAA AGCAGCAGGG CTACGCGGCC
    TGCCAGACCA CCAAGAAGGT GTCGCGGCTG GAGTGCAGGG GCGGCTGCGC GGCCGGGCAG
    TGCTGCGGGC CGCTGCGGAG CAAGCGGCGG AAATACTCCT TCGAGTGCAC GGACGGCTCG
    TCGTTCGTGG ACGAGGTGGA GAAGGTGGTC AAGTGCGGCT GCAGCAGGTG CGCCGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968150.1
    CDS213..4772
    Translation

    Target ORF information:

    RefSeq Version XM_014112675.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112675.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
    GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC
    TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG
    GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC
    CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG
    GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG
    TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG
    GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC
    AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC
    ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT
    TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG
    GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT
    TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG
    AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT
    CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC
    TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT
    GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC
    AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC
    TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC
    CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG
    TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA
    AGCGGGGACT GCTTTGCAGA TCTGGCTTGC CCTGAAAAGT GCCGCTGTGA AGGAACCACA
    GTAGATTGCT CCAATCAAAA ACTCACCAAA ATCCCAGACC ACATCCCCCA GTACACTGCA
    GAGCTGCGTC TCAATAATAA TGAATTCACA GTGCTGGAAG CTACAGGAAT CTTCAAGAAA
    CTTCCGCAGT TACGTAAAAT AAACTTCAGC AACAACAAGA TCACAGACAT TGAAGAAGGA
    GCATTTGAAG GAGCAGCTGG TGTAAACGAA ATCCTTCTCA CGAGTAACCG TTTGGAAAAT
    GTTCAGCATA AGATGTTCAA GGGATTGGAA AGCCTGAAAA CGTTGATGTT GCGAAGCAAT
    CGCATAAGCT GCGTTGGCAA CGATAGCTTC ATAGGCCTGA GCTCTGTGCG GTTGCTTTCG
    CTGTACGATA ATCAGATCGC CACCATCGCG CCGGGGGCGT TCGACACCCT GCACTCGTTG
    TCCACCCTAA ACCTGTTGGC CAACCCTTTT AACTGCAACT GCTACCTGGC TTGGCTGGGC
    GAGTGGCTCA GGAAGAAAAG AATTGTAACC GGAAATCCTC GCTGTCAAAA ACCATACTTC
    CTCAAAGAAA TCCCCATCCA GGACGTCGCC ATTCAAGACT TCACGTGTGA CGACGGAAAT
    GACGACAGTA GCTGTTCTCC ACTCTCGCGC TGTCCCACGG AATGCACGTG CTTGGATACA
    GTTGTCCGAT GTAGCAACAA GGGCCTGAAG GTCTTGCCCA AAGGTATTCC CAGAGACGTC
    ACTGAACTGT ATCTGGATGG GAACCACTTT ACCTTGGTTC CCAAGGAGCT CTATAACTAC
    AAACATCTAA CGCTTATAGA CCTGAGCAAC AACCGCATAA GCACTCTTTC TAATCAGAGC
    TTCAGCAACA TGACCCAGCT CCTCACCCTA ATTCTCAGTT ACAACCGTTT GAGATGTATT
    CCTCCTCGAA CCTTCGATGG ACTCAAGTCT CTCCGATTAC TTTCATTACA TGGAAATGAC
    ATTTCTGTTG TGCCTGAAGG TGCTTTCAGT GATCTCTCTG CATTATCACA CCTAGCAATC
    GGAGCCAACC CCCTTTACTG TGATTGCAAC ATGCAGTGGT TATCGGACTG GGTAAAGTCG
    GAATACAAAG AACCCGGGAT TGCTCGCTGT GCCGGCCCCG GAGAAATGGC AGATAAATTA
    TTACTCACGA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CTGTGGATAT CAATATTCTA
    GCTAAATGTA ATCCCTGCTT ATCAAACCCA TGTAAGAATG ATGGCACCTG TAACAATGAT
    CCAGTCGACT TTTATCGCTG TACCTGTCCG TATGGTTTCA AGGGGCAGGA CTGTGATGTC
    CCAATCCACG CATGCATCAG TAACCCGTGT ACACATGGAG GAACTTGCCA CTTAAAGGAG
    GGAGAAAAAG ATGGATTCTG GTGTATTTGT GCCGATGGAT TTGAAGGAGA AAATTGTGAA
    GTCAATGTTG ATGACTGTGA AGATAATGAC TGTGAAAATA ACTCTACGTG TGTCGATGGA
    ATTAATAACT ACACATGCCT TTGTCCGCCT GAGTACACAG GCGAGTTGTG TGAGGAGAAG
    CTGGACTTCT GCGCTCAGGA CCTGAACCCC TGCCAGCACG ACTCCAAGTG CATCCTGATG
    CCCAAAGGAT TCAAATGCGA CTGCACGCCG GGGTACGTGG GCGAGCACTG CGACATCGAC
    TTCGACGACT GCCAGGATCA CAAGTGTAAA AACGGAGCGC ACTGCACGGA CGCGGTGAAC
    GGCTACACGT GCACCTGCCC CGAAGGCTAC AGCGGCTTGT TCTGTGAATT CTCCCCGCCC
    ATGGTCCTCC CACGCACCAG CCCCTGTGAC AACTTCGACT GTCAGAACGG GGCGCAGTGC
    ATCGTCAGGG CGGGCGAGCC AATCTGCCAG TGTCTGCCCG GCTACCAGGG GGACAAGTGT
    GAGAAGTTGG TCAGCGTGAA CTTCGTGAAC AAAGAGTCGT ATCTTCAAAT TCCTTCAGCC
    AAGGTCCGGC CCCAAACGAA CATCACCCTG CAGATTGCCA CCGACGAAGA CAGCGGGATC
    CTCCTGTACA AGGGCGACAA GGACCACATT GCCGTGGAGC TGTATCGGGG ACGGGTGCGC
    GCCAGCTACG ACACCGGCTC GCACCCCGCT TCTGCCATTT ACAGCGTGGA GACGATCAAT
    GATGGAAACT TTCACATTGT GGAACTACTT GCCCTGGATC AGAGCCTGTC CCTCTCCGTG
    GATGGAGGGA GCCCCAAAAT CATCACCAAC TTGTCAAAGC AGTCCACTCT GAATTTTGAC
    TCTCCACTCT ATGTAGGAGG CATGCCCGGG AGGAACAACG TGGCCGCGGC CCTGCGCCAG
    GCCCCGGGGC ACAACGGCAC CAGCTTCCAC GGCTGCATCC GGAACCTGTA TATCAACAGC
    GAGCTCCAGG ACTTCCGCCA GGTGCCCATG CAGACCGGCA TCCTGCCCGG CTGCGAGCCG
    TGCCACAGGA AGGTGTGTGC CCACGGCGCG TGCCAGCCCA GCAGCCAGTC GGGCTTCACC
    TGCGAGTGCG AGGAGGGCTG GACGGGGCCC CTGTGTGACC AGAGGACCAA CGACCCCTGT
    CTCGGGAACA ACTGTAAGTG TCTGGAGGGC CACGGGGGCG TCCTCTGCGA CGAAGAGGAG
    GACCTGTTCA ACCCCTGCCA GGCCATCAGG TGCAAGCACG GGAAATGCAG GCTCTCGGGC
    CTGGGCCAGC CCTACTGCGA ATGCAGCAGC GGGTACACGG GGGATAGCTG CGACCGAGAA
    GTGTCCTGTC GGGGCGAGCG CGTCCGGGAC TACTACCCAA AGCAGCAGGG CTACGCGGCC
    TGCCAGACCA CCAAGAAGGT GTCGCGGCTG GAGTGCAGGG GCGGCTGCGC GGCCGGGCAG
    TGCTGCGGGC CGCTGCGGAG CAAGCGGCGG AAATACTCCT TCGAGTGCAC GGACGGCTCG
    TCGTTCGTGG ACGAGGTGGA GAAGGTGGTC AAGTGCGGCT GCAGCAGGTG CGCCGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35889
    Clone ID Related Accession (Same CDS sequence) XM_014112675.2 , XM_014112675.1
    Accession Version XM_014112675.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4560bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product slit homolog 2 protein isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
    GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC
    TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG
    GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC
    CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG
    GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG
    TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG
    GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC
    AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC
    ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT
    TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG
    GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT
    TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG
    AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT
    CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC
    TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT
    GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC
    AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC
    TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC
    CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG
    TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA
    AGCGGGGACT GCTTTGCAGA TCTGGCTTGC CCTGAAAAGT GCCGCTGTGA AGGAACCACA
    GTAGATTGCT CCAATCAAAA ACTCACCAAA ATCCCAGACC ACATCCCCCA GTACACTGCA
    GAGCTGCGTC TCAATAATAA TGAATTCACA GTGCTGGAAG CTACAGGAAT CTTCAAGAAA
    CTTCCGCAGT TACGTAAAAT AAACTTCAGC AACAACAAGA TCACAGACAT TGAAGAAGGA
    GCATTTGAAG GAGCAGCTGG TGTAAACGAA ATCCTTCTCA CGAGTAACCG TTTGGAAAAT
    GTTCAGCATA AGATGTTCAA GGGATTGGAA AGCCTGAAAA CGTTGATGTT GCGAAGCAAT
    CGCATAAGCT GCGTTGGCAA CGATAGCTTC ATAGGCCTGA GCTCTGTGCG GTTGCTTTCG
    CTGTACGATA ATCAGATCGC CACCATCGCG CCGGGGGCGT TCGACACCCT GCACTCGTTG
    TCCACCCTAA ACCTGTTGGC CAACCCTTTT AACTGCAACT GCTACCTGGC TTGGCTGGGC
    GAGTGGCTCA GGAAGAAAAG AATTGTAACC GGAAATCCTC GCTGTCAAAA ACCATACTTC
    CTCAAAGAAA TCCCCATCCA GGACGTCGCC ATTCAAGACT TCACGTGTGA CGACGGAAAT
    GACGACAGTA GCTGTTCTCC ACTCTCGCGC TGTCCCACGG AATGCACGTG CTTGGATACA
    GTTGTCCGAT GTAGCAACAA GGGCCTGAAG GTCTTGCCCA AAGGTATTCC CAGAGACGTC
    ACTGAACTGT ATCTGGATGG GAACCACTTT ACCTTGGTTC CCAAGGAGCT CTATAACTAC
    AAACATCTAA CGCTTATAGA CCTGAGCAAC AACCGCATAA GCACTCTTTC TAATCAGAGC
    TTCAGCAACA TGACCCAGCT CCTCACCCTA ATTCTCAGTT ACAACCGTTT GAGATGTATT
    CCTCCTCGAA CCTTCGATGG ACTCAAGTCT CTCCGATTAC TTTCATTACA TGGAAATGAC
    ATTTCTGTTG TGCCTGAAGG TGCTTTCAGT GATCTCTCTG CATTATCACA CCTAGCAATC
    GGAGCCAACC CCCTTTACTG TGATTGCAAC ATGCAGTGGT TATCGGACTG GGTAAAGTCG
    GAATACAAAG AACCCGGGAT TGCTCGCTGT GCCGGCCCCG GAGAAATGGC AGATAAATTA
    TTACTCACGA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CTGTGGATAT CAATATTCTA
    GCTAAATGTA ATCCCTGCTT ATCAAACCCA TGTAAGAATG ATGGCACCTG TAACAATGAT
    CCAGTCGACT TTTATCGCTG TACCTGTCCG TATGGTTTCA AGGGGCAGGA CTGTGATGTC
    CCAATCCACG CATGCATCAG TAACCCGTGT ACACATGGAG GAACTTGCCA CTTAAAGGAG
    GGAGAAAAAG ATGGATTCTG GTGTATTTGT GCCGATGGAT TTGAAGGAGA AAATTGTGAA
    GTCAATGTTG ATGACTGTGA AGATAATGAC TGTGAAAATA ACTCTACGTG TGTCGATGGA
    ATTAATAACT ACACATGCCT TTGTCCGCCT GAGTACACAG GCGAGTTGTG TGAGGAGAAG
    CTGGACTTCT GCGCTCAGGA CCTGAACCCC TGCCAGCACG ACTCCAAGTG CATCCTGATG
    CCCAAAGGAT TCAAATGCGA CTGCACGCCG GGGTACGTGG GCGAGCACTG CGACATCGAC
    TTCGACGACT GCCAGGATCA CAAGTGTAAA AACGGAGCGC ACTGCACGGA CGCGGTGAAC
    GGCTACACGT GCACCTGCCC CGAAGGCTAC AGCGGCTTGT TCTGTGAATT CTCCCCGCCC
    ATGGTCCTCC CACGCACCAG CCCCTGTGAC AACTTCGACT GTCAGAACGG GGCGCAGTGC
    ATCGTCAGGG CGGGCGAGCC AATCTGCCAG TGTCTGCCCG GCTACCAGGG GGACAAGTGT
    GAGAAGTTGG TCAGCGTGAA CTTCGTGAAC AAAGAGTCGT ATCTTCAAAT TCCTTCAGCC
    AAGGTCCGGC CCCAAACGAA CATCACCCTG CAGATTGCCA CCGACGAAGA CAGCGGGATC
    CTCCTGTACA AGGGCGACAA GGACCACATT GCCGTGGAGC TGTATCGGGG ACGGGTGCGC
    GCCAGCTACG ACACCGGCTC GCACCCCGCT TCTGCCATTT ACAGCGTGGA GACGATCAAT
    GATGGAAACT TTCACATTGT GGAACTACTT GCCCTGGATC AGAGCCTGTC CCTCTCCGTG
    GATGGAGGGA GCCCCAAAAT CATCACCAAC TTGTCAAAGC AGTCCACTCT GAATTTTGAC
    TCTCCACTCT ATGTAGGAGG CATGCCCGGG AGGAACAACG TGGCCGCGGC CCTGCGCCAG
    GCCCCGGGGC ACAACGGCAC CAGCTTCCAC GGCTGCATCC GGAACCTGTA TATCAACAGC
    GAGCTCCAGG ACTTCCGCCA GGTGCCCATG CAGACCGGCA TCCTGCCCGG CTGCGAGCCG
    TGCCACAGGA AGGTGTGTGC CCACGGCGCG TGCCAGCCCA GCAGCCAGTC GGGCTTCACC
    TGCGAGTGCG AGGAGGGCTG GACGGGGCCC CTGTGTGACC AGAGGACCAA CGACCCCTGT
    CTCGGGAACA ACTGTAAGTG TCTGGAGGGC CACGGGGGCG TCCTCTGCGA CGAAGAGGAG
    GACCTGTTCA ACCCCTGCCA GGCCATCAGG TGCAAGCACG GGAAATGCAG GCTCTCGGGC
    CTGGGCCAGC CCTACTGCGA ATGCAGCAGC GGGTACACGG GGGATAGCTG CGACCGAGAA
    GTGTCCTGTC GGGGCGAGCG CGTCCGGGAC TACTACCCAA AGCAGCAGGG CTACGCGGCC
    TGCCAGACCA CCAAGAAGGT GTCGCGGCTG GAGTGCAGGG GCGGCTGCGC GGCCGGGCAG
    TGCTGCGGGC CGCTGCGGAG CAAGCGGCGG AAATACTCCT TCGAGTGCAC GGACGGCTCG
    TCGTTCGTGG ACGAGGTGGA GAAGGTGGTC AAGTGCGGCT GCAGCAGGTG CGCCGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968150.1
    CDS86..4645
    Translation

    Target ORF information:

    RefSeq Version XM_014112675.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112675.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
    GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC
    TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG
    GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC
    CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG
    GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG
    TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG
    GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC
    AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC
    ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT
    TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG
    GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT
    TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG
    AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT
    CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC
    TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT
    GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC
    AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC
    TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC
    CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG
    TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA
    AGCGGGGACT GCTTTGCAGA TCTGGCTTGC CCTGAAAAGT GCCGCTGTGA AGGAACCACA
    GTAGATTGCT CCAATCAAAA ACTCACCAAA ATCCCAGACC ACATCCCCCA GTACACTGCA
    GAGCTGCGTC TCAATAATAA TGAATTCACA GTGCTGGAAG CTACAGGAAT CTTCAAGAAA
    CTTCCGCAGT TACGTAAAAT AAACTTCAGC AACAACAAGA TCACAGACAT TGAAGAAGGA
    GCATTTGAAG GAGCAGCTGG TGTAAACGAA ATCCTTCTCA CGAGTAACCG TTTGGAAAAT
    GTTCAGCATA AGATGTTCAA GGGATTGGAA AGCCTGAAAA CGTTGATGTT GCGAAGCAAT
    CGCATAAGCT GCGTTGGCAA CGATAGCTTC ATAGGCCTGA GCTCTGTGCG GTTGCTTTCG
    CTGTACGATA ATCAGATCGC CACCATCGCG CCGGGGGCGT TCGACACCCT GCACTCGTTG
    TCCACCCTAA ACCTGTTGGC CAACCCTTTT AACTGCAACT GCTACCTGGC TTGGCTGGGC
    GAGTGGCTCA GGAAGAAAAG AATTGTAACC GGAAATCCTC GCTGTCAAAA ACCATACTTC
    CTCAAAGAAA TCCCCATCCA GGACGTCGCC ATTCAAGACT TCACGTGTGA CGACGGAAAT
    GACGACAGTA GCTGTTCTCC ACTCTCGCGC TGTCCCACGG AATGCACGTG CTTGGATACA
    GTTGTCCGAT GTAGCAACAA GGGCCTGAAG GTCTTGCCCA AAGGTATTCC CAGAGACGTC
    ACTGAACTGT ATCTGGATGG GAACCACTTT ACCTTGGTTC CCAAGGAGCT CTATAACTAC
    AAACATCTAA CGCTTATAGA CCTGAGCAAC AACCGCATAA GCACTCTTTC TAATCAGAGC
    TTCAGCAACA TGACCCAGCT CCTCACCCTA ATTCTCAGTT ACAACCGTTT GAGATGTATT
    CCTCCTCGAA CCTTCGATGG ACTCAAGTCT CTCCGATTAC TTTCATTACA TGGAAATGAC
    ATTTCTGTTG TGCCTGAAGG TGCTTTCAGT GATCTCTCTG CATTATCACA CCTAGCAATC
    GGAGCCAACC CCCTTTACTG TGATTGCAAC ATGCAGTGGT TATCGGACTG GGTAAAGTCG
    GAATACAAAG AACCCGGGAT TGCTCGCTGT GCCGGCCCCG GAGAAATGGC AGATAAATTA
    TTACTCACGA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CTGTGGATAT CAATATTCTA
    GCTAAATGTA ATCCCTGCTT ATCAAACCCA TGTAAGAATG ATGGCACCTG TAACAATGAT
    CCAGTCGACT TTTATCGCTG TACCTGTCCG TATGGTTTCA AGGGGCAGGA CTGTGATGTC
    CCAATCCACG CATGCATCAG TAACCCGTGT ACACATGGAG GAACTTGCCA CTTAAAGGAG
    GGAGAAAAAG ATGGATTCTG GTGTATTTGT GCCGATGGAT TTGAAGGAGA AAATTGTGAA
    GTCAATGTTG ATGACTGTGA AGATAATGAC TGTGAAAATA ACTCTACGTG TGTCGATGGA
    ATTAATAACT ACACATGCCT TTGTCCGCCT GAGTACACAG GCGAGTTGTG TGAGGAGAAG
    CTGGACTTCT GCGCTCAGGA CCTGAACCCC TGCCAGCACG ACTCCAAGTG CATCCTGATG
    CCCAAAGGAT TCAAATGCGA CTGCACGCCG GGGTACGTGG GCGAGCACTG CGACATCGAC
    TTCGACGACT GCCAGGATCA CAAGTGTAAA AACGGAGCGC ACTGCACGGA CGCGGTGAAC
    GGCTACACGT GCACCTGCCC CGAAGGCTAC AGCGGCTTGT TCTGTGAATT CTCCCCGCCC
    ATGGTCCTCC CACGCACCAG CCCCTGTGAC AACTTCGACT GTCAGAACGG GGCGCAGTGC
    ATCGTCAGGG CGGGCGAGCC AATCTGCCAG TGTCTGCCCG GCTACCAGGG GGACAAGTGT
    GAGAAGTTGG TCAGCGTGAA CTTCGTGAAC AAAGAGTCGT ATCTTCAAAT TCCTTCAGCC
    AAGGTCCGGC CCCAAACGAA CATCACCCTG CAGATTGCCA CCGACGAAGA CAGCGGGATC
    CTCCTGTACA AGGGCGACAA GGACCACATT GCCGTGGAGC TGTATCGGGG ACGGGTGCGC
    GCCAGCTACG ACACCGGCTC GCACCCCGCT TCTGCCATTT ACAGCGTGGA GACGATCAAT
    GATGGAAACT TTCACATTGT GGAACTACTT GCCCTGGATC AGAGCCTGTC CCTCTCCGTG
    GATGGAGGGA GCCCCAAAAT CATCACCAAC TTGTCAAAGC AGTCCACTCT GAATTTTGAC
    TCTCCACTCT ATGTAGGAGG CATGCCCGGG AGGAACAACG TGGCCGCGGC CCTGCGCCAG
    GCCCCGGGGC ACAACGGCAC CAGCTTCCAC GGCTGCATCC GGAACCTGTA TATCAACAGC
    GAGCTCCAGG ACTTCCGCCA GGTGCCCATG CAGACCGGCA TCCTGCCCGG CTGCGAGCCG
    TGCCACAGGA AGGTGTGTGC CCACGGCGCG TGCCAGCCCA GCAGCCAGTC GGGCTTCACC
    TGCGAGTGCG AGGAGGGCTG GACGGGGCCC CTGTGTGACC AGAGGACCAA CGACCCCTGT
    CTCGGGAACA ACTGTAAGTG TCTGGAGGGC CACGGGGGCG TCCTCTGCGA CGAAGAGGAG
    GACCTGTTCA ACCCCTGCCA GGCCATCAGG TGCAAGCACG GGAAATGCAG GCTCTCGGGC
    CTGGGCCAGC CCTACTGCGA ATGCAGCAGC GGGTACACGG GGGATAGCTG CGACCGAGAA
    GTGTCCTGTC GGGGCGAGCG CGTCCGGGAC TACTACCCAA AGCAGCAGGG CTACGCGGCC
    TGCCAGACCA CCAAGAAGGT GTCGCGGCTG GAGTGCAGGG GCGGCTGCGC GGCCGGGCAG
    TGCTGCGGGC CGCTGCGGAG CAAGCGGCGG AAATACTCCT TCGAGTGCAC GGACGGCTCG
    TCGTTCGTGG ACGAGGTGGA GAAGGTGGTC AAGTGCGGCT GCAGCAGGTG CGCCGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.