LRRC8A cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LRRC8A
Entrez Gene ID	491309
Full Name	leucine rich repeat containing 8 VRAC subunit A
General protein information	Preferred Names leucine rich repeat containing 8 VRAC subunit A Names volume-regulated anion channel subunit LRRC8A leucine rich repeat containing 8 family member A
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_548430.4	XP_548430.2	volume-regulated anion channel subunit LRRC8A
XM_548430.5	XP_548430.2	volume-regulated anion channel subunit LRRC8A
XM_005625263.2	XP_005625320.1	volume-regulated anion channel subunit LRRC8A
XM_005625265.3	XP_005625322.1	volume-regulated anion channel subunit LRRC8A
XM_005625263.3	XP_005625320.1	volume-regulated anion channel subunit LRRC8A
XM_005625265.2	XP_005625322.1	volume-regulated anion channel subunit LRRC8A
XM_005625264.2	XP_005625321.1	volume-regulated anion channel subunit LRRC8A
XM_022423747.1	XP_022279455.1	volume-regulated anion channel subunit LRRC8A

Pathways from BioSystems

Homologies

Homo sapiens (human)	LRRC8A	NP_001120717.1
Bos taurus (cattle)	LRRC8A	NP_001070275.1
Danio rerio (zebrafish)	lrrc8a	NP_001025120.1
Danio rerio (zebrafish)	si:ch211-236k19.7	XP_005165659.1
Xenopus tropicalis (tropical clawed frog)	lrrc8a	XP_004916747.1
	LRRC8A	XP_001109698.1
Mus musculus (house mouse)	Lrrc8a	NP_808393.1
Gallus gallus (chicken)	LOC100858917	XP_003642331.1
Gallus gallus (chicken)	LRRC8A	XP_415484.1
Pan troglodytes (chimpanzee)	LRRC8A	XP_520301.1
Canis lupus familiaris (dog)	LRRC8A	XP_005625321.1
Rattus norvegicus (Norway rat)	Lrrc8a	NP_001019953.1

Gene Ontology

The following LRRC8A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LRRC8A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe12734	XM_022423747.1 Latest version!	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit A (LRRC8A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe12734	XM_005625264.2 Latest version!	Canis lupus familiaris leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe12734	XM_548430.5 Latest version!	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit A (LRRC8A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe12734	XM_005625265.3 Latest version!	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit A (LRRC8A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe12734	XM_005625263.3 Latest version!	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit A (LRRC8A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
OCe12734	XM_548430.4	Canis lupus familiaris leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe12734	XM_005625263.2	Canis lupus familiaris leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe12734	XM_005625265.2	Canis lupus familiaris leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LRRC8A ( XM_548430.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_548430.2 Canis lupus familiaris leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe12734
Clone ID Related Accession (Same CDS sequence)	XM_548430.4 , XM_548430.5 , XM_005625263.2 , XM_005625265.3 , XM_005625263.3 , XM_005625265.2 , XM_005625264.2 , XM_022423747.1
Accession Version	XM_548430.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	volume-regulated anion channel subunit LRRC8A
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_548430.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCCAG TGACAGAGCT CCGCTACTTT GCAGACACGC AGCCAGCGTA CCGGATCCTG 
AAGCCATGGT GGGACGTGTT CACAGACTAT ATCTCCATTG TGATGTTGAT GATCGCAGTC 
TTTGGGGGCA CGCTGCAGGT CACCCAGGAC AAGATGATCT GCCTGCCTTG TAAGTGGGTC 
ACCAAGGATT CCTGCAATGA CTCATTCCGA GGCTGGGCAG CCCCAGGCCC AGAGCCCACC 
TACCCAAACT CCACGGTCCC ACCGCCCCCT GGCACAGGCC CCACAGGCAT CAAGTACGAT 
CTCGACCGGC ACCAATACAA CTACGTGGAT GCTGTGTGCT ACGAGAACCG CCTGCACTGG 
TTTGCCAAGT ACTTCCCCTA TCTGGTGCTC CTGCACACGC TCATCTTCCT GGCCTGTAGC 
AACTTCTGGT TCAAGTTTCC GCGCACCAGC TCGAAGCTAG AGCACTTTGT GTCTATCCTG 
CTCAAGTGCT TTGACTCACC ATGGACTACG CGGGCCCTGT CAGAGACGGT GGTGGAAGAG 
AGCGACCCCA AGCCGGCCTT CAGCAAGATG AATGGCTCCA TGGACAAGAA GTCGTCGACG 
GTCAGTGAAG ATGTGGAGGC CACTGTGCCC ATGCTGCAGC GGACCAAGTC ACGGATCGAG 
CAGGGCATTG TGGACCGCTC AGAGACAGGT GTGCTGGACA AGAAAGAGGG GGAGCAGGCC 
AAGGCGCTGT TTGAGAAGGT CAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG 
TACCGCCTCT ATATGCGGCA GACCATCATC AAGGTGATTA AGTTCATGCT CATCATCTGC 
TACACTGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGTACAGT CGACATCGAG 
AGCCTGACGG GCTATCGCAC CTACCGCTGT GCCCACCCCC TGGCCACGCT CTTCAAGATC 
CTGGCATCCT TCTATATTAG CTTGGTCATC TTCTATGGGC TCATCTGCAT GTACACACTG 
TGGTGGATGT TGCGACGGTC CCTCAAGAAG TACTCATTTG AGTCGATCCG TGAGGAGAGC 
AGTTATAGTG ACATCCCCGA CGTCAAGAAT GACTTTGCCT TTATGCTCCA CCTCATCGAC 
CAGTACGACC CACTCTACTC AAAGCGCTTT GCTGTCTTCC TCTCGGAAGT GAGTGAGAAC 
AAGCTGCGGC AGCTGAACCT CAATAATGAG TGGACACTGG ACAAGCTGCG CCAGCGGCTC 
ACGAAGAATG CACAGGACAA GTTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC 
ACCGTGTTCG ATCTGGTGGA GCTAGAGGTG CTGAAGCTGG AGCTGATCCC AGACGTGACC 
ATTCCGCCCA GCATTGCGCA GCTCACAGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCA 
GCCAAGATTG AGGCACCTGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC CCTGCACATC 
AAGTTCACGG ACATCAAGGA GATCCCACTG TGGATCTACA GCCTGAAGAC GCTAGAGGAG 
CTGCACTTGA CGGGCAACCT GAGTGCTGAC AACAACCGCT ACATCGTCAT TGACGGGCTG 
CGTGAGCTCA AGCGCCTCAA GGTGCTGCGG CTCAAGAGCA ATCTGAGTAA GCTGCCACAA 
GTGGTCACCG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACCAAG 
CTCATCGTCC TCAATAGCCT CAAGAAGATG GTGAACCTGA CGGAGCTGGA GCTGATACGC 
TGCGACCTGG AGCGGATCCC GCACTCCATC TTTAGCCTCC ATAACCTGCA GGAGATCGAC 
CTCAAGGACA ACAACCTCAA GACCATCGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC 
CTCACCTGCC TTAAGCTGTG GTACAACCAC ATCGCCTACA TCCCCATCCA GATTGGCAAT 
CTCACCAACC TTGAGCGCCT CTACCTAAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG 
CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAATTT GACCTTCCTC 
CCTGCCGACA TCGGCCTCTT GCAGAACCTC CAGAACCTGG CTGTCACGGC CAACCGGATT 
GAGGCGCTGC CCCCGGAACT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC 
AACGTGCTGC AGTCCCTGCC CTCGAGGGTG GGTGAGCTGA CCAACCTAAC CCAGATTGAG 
CTGCGGGGCA ACCGGCTGGA GTGCCTGCCC GTGGAGCTGG GCGAGTGCCC ACTGCTCAAG 
CGCAGCGGCC TGGTGGTTGA GGAGGATCTG TTCAATACGT TGCCACCTGA GGTGAAGGAA 
CGGCTGTGGA GGGCCGACAA GGAGCAGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_548430.2
CDS	140..2572
Translation	MIPVTELRYFADTQPAYRILKPWWDVFTDYISIVMLMIAVFGGTLQVTQDKMICLPCKWVTKDSCNDSFRGWAAPGPEPTYPNSTVPPPPGTGPTGIKYDLDRHQYNYVDAVCYENRLHWFAKYFPYLVLLHTLIFLACSNFWFKFPRTSSKLEHFVSILLKCFDSPWTTRALSETVVEESDPKPAFSKMNGSMDKKSSTVSEDVEATVPMLQRTKSRIEQGIVDRSETGVLDKKEGEQAKALFEKVKKFRTHVEEGDIVYRLYMRQTIIKVIKFMLIICYTVYYVHNIKFDVDCTVDIESLTGYRTYRCAHPLATLFKILASFYISLVIFYGLICMYTLWWMLRRSLKKYSFESIREESSYSDIPDVKNDFAFMLHLIDQYDPLYSKRFAVFLSEVSENKLRQLNLNNEWTLDKLRQRLTKNAQDKLELHLFMLSGIPDTVFDLVELEVLKLELIPDVTIPPSIAQLTGLKELWLYHTAAKIEAPALAFLRENLRALHIKFTDIKEIPLWIYSLKTLEELHLTGNLSADNNRYIVIDGLRELKRLKVLRLKSNLSKLPQVVTDVGVHLQKLSINNEGTKLIVLNSLKKMVNLTELELIRCDLERIPHSIFSLHNLQEIDLKDNNLKTIEEIISFQHLHRLTCLKLWYNHIAYIPIQIGNLTNLERLYLNRNKIEKIPTQLFYCRKLRYLDLSHNNLTFLPADIGLLQNLQNLAVTANRIEALPPELFQCRKLRALHLGNNVLQSLPSRVGELTNLTQIELRGNRLECLPVELGECPLLKRSGLVVEEDLFNTLPPEVKERLWRADKEQA

Target ORF information:

RefSeq Version	XM_548430.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_548430.4

ORF Insert Sequence:

ATGATTCCAG TGACAGAGCT CCGCTACTTT GCAGACACGC AGCCAGCGTA CCGGATCCTG 
AAGCCATGGT GGGACGTGTT CACAGACTAT ATCTCCATTG TGATGTTGAT GATCGCAGTC 
TTTGGGGGCA CGCTGCAGGT CACCCAGGAC AAGATGATCT GCCTGCCTTG TAAGTGGGTC 
ACCAAGGATT CCTGCAATGA CTCATTCCGA GGCTGGGCAG CCCCAGGCCC AGAGCCCACC 
TACCCAAACT CCACGGTCCC ACCGCCCCCT GGCACAGGCC CCACAGGCAT CAAGTACGAT 
CTCGACCGGC ACCAATACAA CTACGTGGAT GCTGTGTGCT ACGAGAACCG CCTGCACTGG 
TTTGCCAAGT ACTTCCCCTA TCTGGTGCTC CTGCACACGC TCATCTTCCT GGCCTGTAGC 
AACTTCTGGT TCAAGTTTCC GCGCACCAGC TCGAAGCTAG AGCACTTTGT GTCTATCCTG 
CTCAAGTGCT TTGACTCACC ATGGACTACG CGGGCCCTGT CAGAGACGGT GGTGGAAGAG 
AGCGACCCCA AGCCGGCCTT CAGCAAGATG AATGGCTCCA TGGACAAGAA GTCGTCGACG 
GTCAGTGAAG ATGTGGAGGC CACTGTGCCC ATGCTGCAGC GGACCAAGTC ACGGATCGAG 
CAGGGCATTG TGGACCGCTC AGAGACAGGT GTGCTGGACA AGAAAGAGGG GGAGCAGGCC 
AAGGCGCTGT TTGAGAAGGT CAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG 
TACCGCCTCT ATATGCGGCA GACCATCATC AAGGTGATTA AGTTCATGCT CATCATCTGC 
TACACTGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGTACAGT CGACATCGAG 
AGCCTGACGG GCTATCGCAC CTACCGCTGT GCCCACCCCC TGGCCACGCT CTTCAAGATC 
CTGGCATCCT TCTATATTAG CTTGGTCATC TTCTATGGGC TCATCTGCAT GTACACACTG 
TGGTGGATGT TGCGACGGTC CCTCAAGAAG TACTCATTTG AGTCGATCCG TGAGGAGAGC 
AGTTATAGTG ACATCCCCGA CGTCAAGAAT GACTTTGCCT TTATGCTCCA CCTCATCGAC 
CAGTACGACC CACTCTACTC AAAGCGCTTT GCTGTCTTCC TCTCGGAAGT GAGTGAGAAC 
AAGCTGCGGC AGCTGAACCT CAATAATGAG TGGACACTGG ACAAGCTGCG CCAGCGGCTC 
ACGAAGAATG CACAGGACAA GTTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC 
ACCGTGTTCG ATCTGGTGGA GCTAGAGGTG CTGAAGCTGG AGCTGATCCC AGACGTGACC 
ATTCCGCCCA GCATTGCGCA GCTCACAGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCA 
GCCAAGATTG AGGCACCTGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC CCTGCACATC 
AAGTTCACGG ACATCAAGGA GATCCCACTG TGGATCTACA GCCTGAAGAC GCTAGAGGAG 
CTGCACTTGA CGGGCAACCT GAGTGCTGAC AACAACCGCT ACATCGTCAT TGACGGGCTG 
CGTGAGCTCA AGCGCCTCAA GGTGCTGCGG CTCAAGAGCA ATCTGAGTAA GCTGCCACAA 
GTGGTCACCG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACCAAG 
CTCATCGTCC TCAATAGCCT CAAGAAGATG GTGAACCTGA CGGAGCTGGA GCTGATACGC 
TGCGACCTGG AGCGGATCCC GCACTCCATC TTTAGCCTCC ATAACCTGCA GGAGATCGAC 
CTCAAGGACA ACAACCTCAA GACCATCGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC 
CTCACCTGCC TTAAGCTGTG GTACAACCAC ATCGCCTACA TCCCCATCCA GATTGGCAAT 
CTCACCAACC TTGAGCGCCT CTACCTAAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG 
CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAATTT GACCTTCCTC 
CCTGCCGACA TCGGCCTCTT GCAGAACCTC CAGAACCTGG CTGTCACGGC CAACCGGATT 
GAGGCGCTGC CCCCGGAACT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC 
AACGTGCTGC AGTCCCTGCC CTCGAGGGTG GGTGAGCTGA CCAACCTAAC CCAGATTGAG 
CTGCGGGGCA ACCGGCTGGA GTGCCTGCCC GTGGAGCTGG GCGAGTGCCC ACTGCTCAAG 
CGCAGCGGCC TGGTGGTTGA GGAGGATCTG TTCAATACGT TGCCACCTGA GGTGAAGGAA 
CGGCTGTGGA GGGCCGACAA GGAGCAGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8A ( XM_548430.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_548430.2 Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit A (LRRC8A), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe12734
Clone ID Related Accession (Same CDS sequence)	XM_548430.4 , XM_548430.5 , XM_005625263.2 , XM_005625265.3 , XM_005625263.3 , XM_005625265.2 , XM_005625264.2 , XM_022423747.1
Accession Version	XM_548430.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	volume-regulated anion channel subunit LRRC8A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_548430.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCCAG TGACAGAGCT CCGCTACTTT GCAGACACGC AGCCAGCGTA CCGGATCCTG 
AAGCCATGGT GGGACGTGTT CACAGACTAT ATCTCCATTG TGATGTTGAT GATCGCAGTC 
TTTGGGGGCA CGCTGCAGGT CACCCAGGAC AAGATGATCT GCCTGCCTTG TAAGTGGGTC 
ACCAAGGATT CCTGCAATGA CTCATTCCGA GGCTGGGCAG CCCCAGGCCC AGAGCCCACC 
TACCCAAACT CCACGGTCCC ACCGCCCCCT GGCACAGGCC CCACAGGCAT CAAGTACGAT 
CTCGACCGGC ACCAATACAA CTACGTGGAT GCTGTGTGCT ACGAGAACCG CCTGCACTGG 
TTTGCCAAGT ACTTCCCCTA TCTGGTGCTC CTGCACACGC TCATCTTCCT GGCCTGTAGC 
AACTTCTGGT TCAAGTTTCC GCGCACCAGC TCGAAGCTAG AGCACTTTGT GTCTATCCTG 
CTCAAGTGCT TTGACTCACC ATGGACTACG CGGGCCCTGT CAGAGACGGT GGTGGAAGAG 
AGCGACCCCA AGCCGGCCTT CAGCAAGATG AATGGCTCCA TGGACAAGAA GTCGTCGACG 
GTCAGTGAAG ATGTGGAGGC CACTGTGCCC ATGCTGCAGC GGACCAAGTC ACGGATCGAG 
CAGGGCATTG TGGACCGCTC AGAGACAGGT GTGCTGGACA AGAAAGAGGG GGAGCAGGCC 
AAGGCGCTGT TTGAGAAGGT CAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG 
TACCGCCTCT ATATGCGGCA GACCATCATC AAGGTGATTA AGTTCATGCT CATCATCTGC 
TACACTGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGTACAGT CGACATCGAG 
AGCCTGACGG GCTATCGCAC CTACCGCTGT GCCCACCCCC TGGCCACGCT CTTCAAGATC 
CTGGCATCCT TCTATATTAG CTTGGTCATC TTCTATGGGC TCATCTGCAT GTACACACTG 
TGGTGGATGT TGCGACGGTC CCTCAAGAAG TACTCATTTG AGTCGATCCG TGAGGAGAGC 
AGTTATAGTG ACATCCCCGA CGTCAAGAAT GACTTTGCCT TTATGCTCCA CCTCATCGAC 
CAGTACGACC CACTCTACTC AAAGCGCTTT GCTGTCTTCC TCTCGGAAGT GAGTGAGAAC 
AAGCTGCGGC AGCTGAACCT CAATAATGAG TGGACACTGG ACAAGCTGCG CCAGCGGCTC 
ACGAAGAATG CACAGGACAA GTTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC 
ACCGTGTTCG ATCTGGTGGA GCTAGAGGTG CTGAAGCTGG AGCTGATCCC AGACGTGACC 
ATTCCGCCCA GCATTGCGCA GCTCACAGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCA 
GCCAAGATTG AGGCACCTGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC CCTGCACATC 
AAGTTCACGG ACATCAAGGA GATCCCACTG TGGATCTACA GCCTGAAGAC GCTAGAGGAG 
CTGCACTTGA CGGGCAACCT GAGTGCTGAC AACAACCGCT ACATCGTCAT TGACGGGCTG 
CGTGAGCTCA AGCGCCTCAA GGTGCTGCGG CTCAAGAGCA ATCTGAGTAA GCTGCCACAA 
GTGGTCACCG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACCAAG 
CTCATCGTCC TCAATAGCCT CAAGAAGATG GTGAACCTGA CGGAGCTGGA GCTGATACGC 
TGCGACCTGG AGCGGATCCC GCACTCCATC TTTAGCCTCC ATAACCTGCA GGAGATCGAC 
CTCAAGGACA ACAACCTCAA GACCATCGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC 
CTCACCTGCC TTAAGCTGTG GTACAACCAC ATCGCCTACA TCCCCATCCA GATTGGCAAT 
CTCACCAACC TTGAGCGCCT CTACCTAAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG 
CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAATTT GACCTTCCTC 
CCTGCCGACA TCGGCCTCTT GCAGAACCTC CAGAACCTGG CTGTCACGGC CAACCGGATT 
GAGGCGCTGC CCCCGGAACT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC 
AACGTGCTGC AGTCCCTGCC CTCGAGGGTG GGTGAGCTGA CCAACCTAAC CCAGATTGAG 
CTGCGGGGCA ACCGGCTGGA GTGCCTGCCC GTGGAGCTGG GCGAGTGCCC ACTGCTCAAG 
CGCAGCGGCC TGGTGGTTGA GGAGGATCTG TTCAATACGT TGCCACCTGA GGTGAAGGAA 
CGGCTGTGGA GGGCCGACAA GGAGCAGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_548430.2
CDS	129..2561
Translation	MIPVTELRYFADTQPAYRILKPWWDVFTDYISIVMLMIAVFGGTLQVTQDKMICLPCKWVTKDSCNDSFRGWAAPGPEPTYPNSTVPPPPGTGPTGIKYDLDRHQYNYVDAVCYENRLHWFAKYFPYLVLLHTLIFLACSNFWFKFPRTSSKLEHFVSILLKCFDSPWTTRALSETVVEESDPKPAFSKMNGSMDKKSSTVSEDVEATVPMLQRTKSRIEQGIVDRSETGVLDKKEGEQAKALFEKVKKFRTHVEEGDIVYRLYMRQTIIKVIKFMLIICYTVYYVHNIKFDVDCTVDIESLTGYRTYRCAHPLATLFKILASFYISLVIFYGLICMYTLWWMLRRSLKKYSFESIREESSYSDIPDVKNDFAFMLHLIDQYDPLYSKRFAVFLSEVSENKLRQLNLNNEWTLDKLRQRLTKNAQDKLELHLFMLSGIPDTVFDLVELEVLKLELIPDVTIPPSIAQLTGLKELWLYHTAAKIEAPALAFLRENLRALHIKFTDIKEIPLWIYSLKTLEELHLTGNLSADNNRYIVIDGLRELKRLKVLRLKSNLSKLPQVVTDVGVHLQKLSINNEGTKLIVLNSLKKMVNLTELELIRCDLERIPHSIFSLHNLQEIDLKDNNLKTIEEIISFQHLHRLTCLKLWYNHIAYIPIQIGNLTNLERLYLNRNKIEKIPTQLFYCRKLRYLDLSHNNLTFLPADIGLLQNLQNLAVTANRIEALPPELFQCRKLRALHLGNNVLQSLPSRVGELTNLTQIELRGNRLECLPVELGECPLLKRSGLVVEEDLFNTLPPEVKERLWRADKEQA

Target ORF information:

RefSeq Version	XM_548430.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit A (LRRC8A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_548430.5

ORF Insert Sequence:

ATGATTCCAG TGACAGAGCT CCGCTACTTT GCAGACACGC AGCCAGCGTA CCGGATCCTG 
AAGCCATGGT GGGACGTGTT CACAGACTAT ATCTCCATTG TGATGTTGAT GATCGCAGTC 
TTTGGGGGCA CGCTGCAGGT CACCCAGGAC AAGATGATCT GCCTGCCTTG TAAGTGGGTC 
ACCAAGGATT CCTGCAATGA CTCATTCCGA GGCTGGGCAG CCCCAGGCCC AGAGCCCACC 
TACCCAAACT CCACGGTCCC ACCGCCCCCT GGCACAGGCC CCACAGGCAT CAAGTACGAT 
CTCGACCGGC ACCAATACAA CTACGTGGAT GCTGTGTGCT ACGAGAACCG CCTGCACTGG 
TTTGCCAAGT ACTTCCCCTA TCTGGTGCTC CTGCACACGC TCATCTTCCT GGCCTGTAGC 
AACTTCTGGT TCAAGTTTCC GCGCACCAGC TCGAAGCTAG AGCACTTTGT GTCTATCCTG 
CTCAAGTGCT TTGACTCACC ATGGACTACG CGGGCCCTGT CAGAGACGGT GGTGGAAGAG 
AGCGACCCCA AGCCGGCCTT CAGCAAGATG AATGGCTCCA TGGACAAGAA GTCGTCGACG 
GTCAGTGAAG ATGTGGAGGC CACTGTGCCC ATGCTGCAGC GGACCAAGTC ACGGATCGAG 
CAGGGCATTG TGGACCGCTC AGAGACAGGT GTGCTGGACA AGAAAGAGGG GGAGCAGGCC 
AAGGCGCTGT TTGAGAAGGT CAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG 
TACCGCCTCT ATATGCGGCA GACCATCATC AAGGTGATTA AGTTCATGCT CATCATCTGC 
TACACTGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGTACAGT CGACATCGAG 
AGCCTGACGG GCTATCGCAC CTACCGCTGT GCCCACCCCC TGGCCACGCT CTTCAAGATC 
CTGGCATCCT TCTATATTAG CTTGGTCATC TTCTATGGGC TCATCTGCAT GTACACACTG 
TGGTGGATGT TGCGACGGTC CCTCAAGAAG TACTCATTTG AGTCGATCCG TGAGGAGAGC 
AGTTATAGTG ACATCCCCGA CGTCAAGAAT GACTTTGCCT TTATGCTCCA CCTCATCGAC 
CAGTACGACC CACTCTACTC AAAGCGCTTT GCTGTCTTCC TCTCGGAAGT GAGTGAGAAC 
AAGCTGCGGC AGCTGAACCT CAATAATGAG TGGACACTGG ACAAGCTGCG CCAGCGGCTC 
ACGAAGAATG CACAGGACAA GTTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC 
ACCGTGTTCG ATCTGGTGGA GCTAGAGGTG CTGAAGCTGG AGCTGATCCC AGACGTGACC 
ATTCCGCCCA GCATTGCGCA GCTCACAGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCA 
GCCAAGATTG AGGCACCTGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC CCTGCACATC 
AAGTTCACGG ACATCAAGGA GATCCCACTG TGGATCTACA GCCTGAAGAC GCTAGAGGAG 
CTGCACTTGA CGGGCAACCT GAGTGCTGAC AACAACCGCT ACATCGTCAT TGACGGGCTG 
CGTGAGCTCA AGCGCCTCAA GGTGCTGCGG CTCAAGAGCA ATCTGAGTAA GCTGCCACAA 
GTGGTCACCG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACCAAG 
CTCATCGTCC TCAATAGCCT CAAGAAGATG GTGAACCTGA CGGAGCTGGA GCTGATACGC 
TGCGACCTGG AGCGGATCCC GCACTCCATC TTTAGCCTCC ATAACCTGCA GGAGATCGAC 
CTCAAGGACA ACAACCTCAA GACCATCGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC 
CTCACCTGCC TTAAGCTGTG GTACAACCAC ATCGCCTACA TCCCCATCCA GATTGGCAAT 
CTCACCAACC TTGAGCGCCT CTACCTAAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG 
CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAATTT GACCTTCCTC 
CCTGCCGACA TCGGCCTCTT GCAGAACCTC CAGAACCTGG CTGTCACGGC CAACCGGATT 
GAGGCGCTGC CCCCGGAACT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC 
AACGTGCTGC AGTCCCTGCC CTCGAGGGTG GGTGAGCTGA CCAACCTAAC CCAGATTGAG 
CTGCGGGGCA ACCGGCTGGA GTGCCTGCCC GTGGAGCTGG GCGAGTGCCC ACTGCTCAAG 
CGCAGCGGCC TGGTGGTTGA GGAGGATCTG TTCAATACGT TGCCACCTGA GGTGAAGGAA 
CGGCTGTGGA GGGCCGACAA GGAGCAGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8A ( XM_005625263.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005625320.1 Canis lupus familiaris leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe12734
Clone ID Related Accession (Same CDS sequence)	XM_548430.4 , XM_548430.5 , XM_005625263.2 , XM_005625265.3 , XM_005625263.3 , XM_005625265.2 , XM_005625264.2 , XM_022423747.1
Accession Version	XM_005625263.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	volume-regulated anion channel subunit LRRC8A
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625263.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCCAG TGACAGAGCT CCGCTACTTT GCAGACACGC AGCCAGCGTA CCGGATCCTG 
AAGCCATGGT GGGACGTGTT CACAGACTAT ATCTCCATTG TGATGTTGAT GATCGCAGTC 
TTTGGGGGCA CGCTGCAGGT CACCCAGGAC AAGATGATCT GCCTGCCTTG TAAGTGGGTC 
ACCAAGGATT CCTGCAATGA CTCATTCCGA GGCTGGGCAG CCCCAGGCCC AGAGCCCACC 
TACCCAAACT CCACGGTCCC ACCGCCCCCT GGCACAGGCC CCACAGGCAT CAAGTACGAT 
CTCGACCGGC ACCAATACAA CTACGTGGAT GCTGTGTGCT ACGAGAACCG CCTGCACTGG 
TTTGCCAAGT ACTTCCCCTA TCTGGTGCTC CTGCACACGC TCATCTTCCT GGCCTGTAGC 
AACTTCTGGT TCAAGTTTCC GCGCACCAGC TCGAAGCTAG AGCACTTTGT GTCTATCCTG 
CTCAAGTGCT TTGACTCACC ATGGACTACG CGGGCCCTGT CAGAGACGGT GGTGGAAGAG 
AGCGACCCCA AGCCGGCCTT CAGCAAGATG AATGGCTCCA TGGACAAGAA GTCGTCGACG 
GTCAGTGAAG ATGTGGAGGC CACTGTGCCC ATGCTGCAGC GGACCAAGTC ACGGATCGAG 
CAGGGCATTG TGGACCGCTC AGAGACAGGT GTGCTGGACA AGAAAGAGGG GGAGCAGGCC 
AAGGCGCTGT TTGAGAAGGT CAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG 
TACCGCCTCT ATATGCGGCA GACCATCATC AAGGTGATTA AGTTCATGCT CATCATCTGC 
TACACTGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGTACAGT CGACATCGAG 
AGCCTGACGG GCTATCGCAC CTACCGCTGT GCCCACCCCC TGGCCACGCT CTTCAAGATC 
CTGGCATCCT TCTATATTAG CTTGGTCATC TTCTATGGGC TCATCTGCAT GTACACACTG 
TGGTGGATGT TGCGACGGTC CCTCAAGAAG TACTCATTTG AGTCGATCCG TGAGGAGAGC 
AGTTATAGTG ACATCCCCGA CGTCAAGAAT GACTTTGCCT TTATGCTCCA CCTCATCGAC 
CAGTACGACC CACTCTACTC AAAGCGCTTT GCTGTCTTCC TCTCGGAAGT GAGTGAGAAC 
AAGCTGCGGC AGCTGAACCT CAATAATGAG TGGACACTGG ACAAGCTGCG CCAGCGGCTC 
ACGAAGAATG CACAGGACAA GTTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC 
ACCGTGTTCG ATCTGGTGGA GCTAGAGGTG CTGAAGCTGG AGCTGATCCC AGACGTGACC 
ATTCCGCCCA GCATTGCGCA GCTCACAGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCA 
GCCAAGATTG AGGCACCTGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC CCTGCACATC 
AAGTTCACGG ACATCAAGGA GATCCCACTG TGGATCTACA GCCTGAAGAC GCTAGAGGAG 
CTGCACTTGA CGGGCAACCT GAGTGCTGAC AACAACCGCT ACATCGTCAT TGACGGGCTG 
CGTGAGCTCA AGCGCCTCAA GGTGCTGCGG CTCAAGAGCA ATCTGAGTAA GCTGCCACAA 
GTGGTCACCG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACCAAG 
CTCATCGTCC TCAATAGCCT CAAGAAGATG GTGAACCTGA CGGAGCTGGA GCTGATACGC 
TGCGACCTGG AGCGGATCCC GCACTCCATC TTTAGCCTCC ATAACCTGCA GGAGATCGAC 
CTCAAGGACA ACAACCTCAA GACCATCGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC 
CTCACCTGCC TTAAGCTGTG GTACAACCAC ATCGCCTACA TCCCCATCCA GATTGGCAAT 
CTCACCAACC TTGAGCGCCT CTACCTAAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG 
CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAATTT GACCTTCCTC 
CCTGCCGACA TCGGCCTCTT GCAGAACCTC CAGAACCTGG CTGTCACGGC CAACCGGATT 
GAGGCGCTGC CCCCGGAACT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC 
AACGTGCTGC AGTCCCTGCC CTCGAGGGTG GGTGAGCTGA CCAACCTAAC CCAGATTGAG 
CTGCGGGGCA ACCGGCTGGA GTGCCTGCCC GTGGAGCTGG GCGAGTGCCC ACTGCTCAAG 
CGCAGCGGCC TGGTGGTTGA GGAGGATCTG TTCAATACGT TGCCACCTGA GGTGAAGGAA 
CGGCTGTGGA GGGCCGACAA GGAGCAGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005625320.1
CDS	247..2679
Translation	MIPVTELRYFADTQPAYRILKPWWDVFTDYISIVMLMIAVFGGTLQVTQDKMICLPCKWVTKDSCNDSFRGWAAPGPEPTYPNSTVPPPPGTGPTGIKYDLDRHQYNYVDAVCYENRLHWFAKYFPYLVLLHTLIFLACSNFWFKFPRTSSKLEHFVSILLKCFDSPWTTRALSETVVEESDPKPAFSKMNGSMDKKSSTVSEDVEATVPMLQRTKSRIEQGIVDRSETGVLDKKEGEQAKALFEKVKKFRTHVEEGDIVYRLYMRQTIIKVIKFMLIICYTVYYVHNIKFDVDCTVDIESLTGYRTYRCAHPLATLFKILASFYISLVIFYGLICMYTLWWMLRRSLKKYSFESIREESSYSDIPDVKNDFAFMLHLIDQYDPLYSKRFAVFLSEVSENKLRQLNLNNEWTLDKLRQRLTKNAQDKLELHLFMLSGIPDTVFDLVELEVLKLELIPDVTIPPSIAQLTGLKELWLYHTAAKIEAPALAFLRENLRALHIKFTDIKEIPLWIYSLKTLEELHLTGNLSADNNRYIVIDGLRELKRLKVLRLKSNLSKLPQVVTDVGVHLQKLSINNEGTKLIVLNSLKKMVNLTELELIRCDLERIPHSIFSLHNLQEIDLKDNNLKTIEEIISFQHLHRLTCLKLWYNHIAYIPIQIGNLTNLERLYLNRNKIEKIPTQLFYCRKLRYLDLSHNNLTFLPADIGLLQNLQNLAVTANRIEALPPELFQCRKLRALHLGNNVLQSLPSRVGELTNLTQIELRGNRLECLPVELGECPLLKRSGLVVEEDLFNTLPPEVKERLWRADKEQA

Target ORF information:

RefSeq Version	XM_005625263.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005625263.2

ORF Insert Sequence:

ATGATTCCAG TGACAGAGCT CCGCTACTTT GCAGACACGC AGCCAGCGTA CCGGATCCTG 
AAGCCATGGT GGGACGTGTT CACAGACTAT ATCTCCATTG TGATGTTGAT GATCGCAGTC 
TTTGGGGGCA CGCTGCAGGT CACCCAGGAC AAGATGATCT GCCTGCCTTG TAAGTGGGTC 
ACCAAGGATT CCTGCAATGA CTCATTCCGA GGCTGGGCAG CCCCAGGCCC AGAGCCCACC 
TACCCAAACT CCACGGTCCC ACCGCCCCCT GGCACAGGCC CCACAGGCAT CAAGTACGAT 
CTCGACCGGC ACCAATACAA CTACGTGGAT GCTGTGTGCT ACGAGAACCG CCTGCACTGG 
TTTGCCAAGT ACTTCCCCTA TCTGGTGCTC CTGCACACGC TCATCTTCCT GGCCTGTAGC 
AACTTCTGGT TCAAGTTTCC GCGCACCAGC TCGAAGCTAG AGCACTTTGT GTCTATCCTG 
CTCAAGTGCT TTGACTCACC ATGGACTACG CGGGCCCTGT CAGAGACGGT GGTGGAAGAG 
AGCGACCCCA AGCCGGCCTT CAGCAAGATG AATGGCTCCA TGGACAAGAA GTCGTCGACG 
GTCAGTGAAG ATGTGGAGGC CACTGTGCCC ATGCTGCAGC GGACCAAGTC ACGGATCGAG 
CAGGGCATTG TGGACCGCTC AGAGACAGGT GTGCTGGACA AGAAAGAGGG GGAGCAGGCC 
AAGGCGCTGT TTGAGAAGGT CAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG 
TACCGCCTCT ATATGCGGCA GACCATCATC AAGGTGATTA AGTTCATGCT CATCATCTGC 
TACACTGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGTACAGT CGACATCGAG 
AGCCTGACGG GCTATCGCAC CTACCGCTGT GCCCACCCCC TGGCCACGCT CTTCAAGATC 
CTGGCATCCT TCTATATTAG CTTGGTCATC TTCTATGGGC TCATCTGCAT GTACACACTG 
TGGTGGATGT TGCGACGGTC CCTCAAGAAG TACTCATTTG AGTCGATCCG TGAGGAGAGC 
AGTTATAGTG ACATCCCCGA CGTCAAGAAT GACTTTGCCT TTATGCTCCA CCTCATCGAC 
CAGTACGACC CACTCTACTC AAAGCGCTTT GCTGTCTTCC TCTCGGAAGT GAGTGAGAAC 
AAGCTGCGGC AGCTGAACCT CAATAATGAG TGGACACTGG ACAAGCTGCG CCAGCGGCTC 
ACGAAGAATG CACAGGACAA GTTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC 
ACCGTGTTCG ATCTGGTGGA GCTAGAGGTG CTGAAGCTGG AGCTGATCCC AGACGTGACC 
ATTCCGCCCA GCATTGCGCA GCTCACAGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCA 
GCCAAGATTG AGGCACCTGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC CCTGCACATC 
AAGTTCACGG ACATCAAGGA GATCCCACTG TGGATCTACA GCCTGAAGAC GCTAGAGGAG 
CTGCACTTGA CGGGCAACCT GAGTGCTGAC AACAACCGCT ACATCGTCAT TGACGGGCTG 
CGTGAGCTCA AGCGCCTCAA GGTGCTGCGG CTCAAGAGCA ATCTGAGTAA GCTGCCACAA 
GTGGTCACCG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACCAAG 
CTCATCGTCC TCAATAGCCT CAAGAAGATG GTGAACCTGA CGGAGCTGGA GCTGATACGC 
TGCGACCTGG AGCGGATCCC GCACTCCATC TTTAGCCTCC ATAACCTGCA GGAGATCGAC 
CTCAAGGACA ACAACCTCAA GACCATCGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC 
CTCACCTGCC TTAAGCTGTG GTACAACCAC ATCGCCTACA TCCCCATCCA GATTGGCAAT 
CTCACCAACC TTGAGCGCCT CTACCTAAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG 
CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAATTT GACCTTCCTC 
CCTGCCGACA TCGGCCTCTT GCAGAACCTC CAGAACCTGG CTGTCACGGC CAACCGGATT 
GAGGCGCTGC CCCCGGAACT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC 
AACGTGCTGC AGTCCCTGCC CTCGAGGGTG GGTGAGCTGA CCAACCTAAC CCAGATTGAG 
CTGCGGGGCA ACCGGCTGGA GTGCCTGCCC GTGGAGCTGG GCGAGTGCCC ACTGCTCAAG 
CGCAGCGGCC TGGTGGTTGA GGAGGATCTG TTCAATACGT TGCCACCTGA GGTGAAGGAA 
CGGCTGTGGA GGGCCGACAA GGAGCAGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8A ( XM_005625265.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005625322.1 Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit A (LRRC8A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe12734
Clone ID Related Accession (Same CDS sequence)	XM_548430.4 , XM_548430.5 , XM_005625263.2 , XM_005625265.3 , XM_005625263.3 , XM_005625265.2 , XM_005625264.2 , XM_022423747.1
Accession Version	XM_005625265.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	volume-regulated anion channel subunit LRRC8A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005625265.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCCAG TGACAGAGCT CCGCTACTTT GCAGACACGC AGCCAGCGTA CCGGATCCTG 
AAGCCATGGT GGGACGTGTT CACAGACTAT ATCTCCATTG TGATGTTGAT GATCGCAGTC 
TTTGGGGGCA CGCTGCAGGT CACCCAGGAC AAGATGATCT GCCTGCCTTG TAAGTGGGTC 
ACCAAGGATT CCTGCAATGA CTCATTCCGA GGCTGGGCAG CCCCAGGCCC AGAGCCCACC 
TACCCAAACT CCACGGTCCC ACCGCCCCCT GGCACAGGCC CCACAGGCAT CAAGTACGAT 
CTCGACCGGC ACCAATACAA CTACGTGGAT GCTGTGTGCT ACGAGAACCG CCTGCACTGG 
TTTGCCAAGT ACTTCCCCTA TCTGGTGCTC CTGCACACGC TCATCTTCCT GGCCTGTAGC 
AACTTCTGGT TCAAGTTTCC GCGCACCAGC TCGAAGCTAG AGCACTTTGT GTCTATCCTG 
CTCAAGTGCT TTGACTCACC ATGGACTACG CGGGCCCTGT CAGAGACGGT GGTGGAAGAG 
AGCGACCCCA AGCCGGCCTT CAGCAAGATG AATGGCTCCA TGGACAAGAA GTCGTCGACG 
GTCAGTGAAG ATGTGGAGGC CACTGTGCCC ATGCTGCAGC GGACCAAGTC ACGGATCGAG 
CAGGGCATTG TGGACCGCTC AGAGACAGGT GTGCTGGACA AGAAAGAGGG GGAGCAGGCC 
AAGGCGCTGT TTGAGAAGGT CAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG 
TACCGCCTCT ATATGCGGCA GACCATCATC AAGGTGATTA AGTTCATGCT CATCATCTGC 
TACACTGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGTACAGT CGACATCGAG 
AGCCTGACGG GCTATCGCAC CTACCGCTGT GCCCACCCCC TGGCCACGCT CTTCAAGATC 
CTGGCATCCT TCTATATTAG CTTGGTCATC TTCTATGGGC TCATCTGCAT GTACACACTG 
TGGTGGATGT TGCGACGGTC CCTCAAGAAG TACTCATTTG AGTCGATCCG TGAGGAGAGC 
AGTTATAGTG ACATCCCCGA CGTCAAGAAT GACTTTGCCT TTATGCTCCA CCTCATCGAC 
CAGTACGACC CACTCTACTC AAAGCGCTTT GCTGTCTTCC TCTCGGAAGT GAGTGAGAAC 
AAGCTGCGGC AGCTGAACCT CAATAATGAG TGGACACTGG ACAAGCTGCG CCAGCGGCTC 
ACGAAGAATG CACAGGACAA GTTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC 
ACCGTGTTCG ATCTGGTGGA GCTAGAGGTG CTGAAGCTGG AGCTGATCCC AGACGTGACC 
ATTCCGCCCA GCATTGCGCA GCTCACAGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCA 
GCCAAGATTG AGGCACCTGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC CCTGCACATC 
AAGTTCACGG ACATCAAGGA GATCCCACTG TGGATCTACA GCCTGAAGAC GCTAGAGGAG 
CTGCACTTGA CGGGCAACCT GAGTGCTGAC AACAACCGCT ACATCGTCAT TGACGGGCTG 
CGTGAGCTCA AGCGCCTCAA GGTGCTGCGG CTCAAGAGCA ATCTGAGTAA GCTGCCACAA 
GTGGTCACCG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACCAAG 
CTCATCGTCC TCAATAGCCT CAAGAAGATG GTGAACCTGA CGGAGCTGGA GCTGATACGC 
TGCGACCTGG AGCGGATCCC GCACTCCATC TTTAGCCTCC ATAACCTGCA GGAGATCGAC 
CTCAAGGACA ACAACCTCAA GACCATCGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC 
CTCACCTGCC TTAAGCTGTG GTACAACCAC ATCGCCTACA TCCCCATCCA GATTGGCAAT 
CTCACCAACC TTGAGCGCCT CTACCTAAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG 
CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAATTT GACCTTCCTC 
CCTGCCGACA TCGGCCTCTT GCAGAACCTC CAGAACCTGG CTGTCACGGC CAACCGGATT 
GAGGCGCTGC CCCCGGAACT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC 
AACGTGCTGC AGTCCCTGCC CTCGAGGGTG GGTGAGCTGA CCAACCTAAC CCAGATTGAG 
CTGCGGGGCA ACCGGCTGGA GTGCCTGCCC GTGGAGCTGG GCGAGTGCCC ACTGCTCAAG 
CGCAGCGGCC TGGTGGTTGA GGAGGATCTG TTCAATACGT TGCCACCTGA GGTGAAGGAA 
CGGCTGTGGA GGGCCGACAA GGAGCAGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005625322.1
CDS	294..2726
Translation	MIPVTELRYFADTQPAYRILKPWWDVFTDYISIVMLMIAVFGGTLQVTQDKMICLPCKWVTKDSCNDSFRGWAAPGPEPTYPNSTVPPPPGTGPTGIKYDLDRHQYNYVDAVCYENRLHWFAKYFPYLVLLHTLIFLACSNFWFKFPRTSSKLEHFVSILLKCFDSPWTTRALSETVVEESDPKPAFSKMNGSMDKKSSTVSEDVEATVPMLQRTKSRIEQGIVDRSETGVLDKKEGEQAKALFEKVKKFRTHVEEGDIVYRLYMRQTIIKVIKFMLIICYTVYYVHNIKFDVDCTVDIESLTGYRTYRCAHPLATLFKILASFYISLVIFYGLICMYTLWWMLRRSLKKYSFESIREESSYSDIPDVKNDFAFMLHLIDQYDPLYSKRFAVFLSEVSENKLRQLNLNNEWTLDKLRQRLTKNAQDKLELHLFMLSGIPDTVFDLVELEVLKLELIPDVTIPPSIAQLTGLKELWLYHTAAKIEAPALAFLRENLRALHIKFTDIKEIPLWIYSLKTLEELHLTGNLSADNNRYIVIDGLRELKRLKVLRLKSNLSKLPQVVTDVGVHLQKLSINNEGTKLIVLNSLKKMVNLTELELIRCDLERIPHSIFSLHNLQEIDLKDNNLKTIEEIISFQHLHRLTCLKLWYNHIAYIPIQIGNLTNLERLYLNRNKIEKIPTQLFYCRKLRYLDLSHNNLTFLPADIGLLQNLQNLAVTANRIEALPPELFQCRKLRALHLGNNVLQSLPSRVGELTNLTQIELRGNRLECLPVELGECPLLKRSGLVVEEDLFNTLPPEVKERLWRADKEQA

Target ORF information:

RefSeq Version	XM_005625265.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit A (LRRC8A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005625265.3

ORF Insert Sequence:

ATGATTCCAG TGACAGAGCT CCGCTACTTT GCAGACACGC AGCCAGCGTA CCGGATCCTG 
AAGCCATGGT GGGACGTGTT CACAGACTAT ATCTCCATTG TGATGTTGAT GATCGCAGTC 
TTTGGGGGCA CGCTGCAGGT CACCCAGGAC AAGATGATCT GCCTGCCTTG TAAGTGGGTC 
ACCAAGGATT CCTGCAATGA CTCATTCCGA GGCTGGGCAG CCCCAGGCCC AGAGCCCACC 
TACCCAAACT CCACGGTCCC ACCGCCCCCT GGCACAGGCC CCACAGGCAT CAAGTACGAT 
CTCGACCGGC ACCAATACAA CTACGTGGAT GCTGTGTGCT ACGAGAACCG CCTGCACTGG 
TTTGCCAAGT ACTTCCCCTA TCTGGTGCTC CTGCACACGC TCATCTTCCT GGCCTGTAGC 
AACTTCTGGT TCAAGTTTCC GCGCACCAGC TCGAAGCTAG AGCACTTTGT GTCTATCCTG 
CTCAAGTGCT TTGACTCACC ATGGACTACG CGGGCCCTGT CAGAGACGGT GGTGGAAGAG 
AGCGACCCCA AGCCGGCCTT CAGCAAGATG AATGGCTCCA TGGACAAGAA GTCGTCGACG 
GTCAGTGAAG ATGTGGAGGC CACTGTGCCC ATGCTGCAGC GGACCAAGTC ACGGATCGAG 
CAGGGCATTG TGGACCGCTC AGAGACAGGT GTGCTGGACA AGAAAGAGGG GGAGCAGGCC 
AAGGCGCTGT TTGAGAAGGT CAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG 
TACCGCCTCT ATATGCGGCA GACCATCATC AAGGTGATTA AGTTCATGCT CATCATCTGC 
TACACTGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGTACAGT CGACATCGAG 
AGCCTGACGG GCTATCGCAC CTACCGCTGT GCCCACCCCC TGGCCACGCT CTTCAAGATC 
CTGGCATCCT TCTATATTAG CTTGGTCATC TTCTATGGGC TCATCTGCAT GTACACACTG 
TGGTGGATGT TGCGACGGTC CCTCAAGAAG TACTCATTTG AGTCGATCCG TGAGGAGAGC 
AGTTATAGTG ACATCCCCGA CGTCAAGAAT GACTTTGCCT TTATGCTCCA CCTCATCGAC 
CAGTACGACC CACTCTACTC AAAGCGCTTT GCTGTCTTCC TCTCGGAAGT GAGTGAGAAC 
AAGCTGCGGC AGCTGAACCT CAATAATGAG TGGACACTGG ACAAGCTGCG CCAGCGGCTC 
ACGAAGAATG CACAGGACAA GTTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC 
ACCGTGTTCG ATCTGGTGGA GCTAGAGGTG CTGAAGCTGG AGCTGATCCC AGACGTGACC 
ATTCCGCCCA GCATTGCGCA GCTCACAGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCA 
GCCAAGATTG AGGCACCTGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC CCTGCACATC 
AAGTTCACGG ACATCAAGGA GATCCCACTG TGGATCTACA GCCTGAAGAC GCTAGAGGAG 
CTGCACTTGA CGGGCAACCT GAGTGCTGAC AACAACCGCT ACATCGTCAT TGACGGGCTG 
CGTGAGCTCA AGCGCCTCAA GGTGCTGCGG CTCAAGAGCA ATCTGAGTAA GCTGCCACAA 
GTGGTCACCG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACCAAG 
CTCATCGTCC TCAATAGCCT CAAGAAGATG GTGAACCTGA CGGAGCTGGA GCTGATACGC 
TGCGACCTGG AGCGGATCCC GCACTCCATC TTTAGCCTCC ATAACCTGCA GGAGATCGAC 
CTCAAGGACA ACAACCTCAA GACCATCGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC 
CTCACCTGCC TTAAGCTGTG GTACAACCAC ATCGCCTACA TCCCCATCCA GATTGGCAAT 
CTCACCAACC TTGAGCGCCT CTACCTAAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG 
CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAATTT GACCTTCCTC 
CCTGCCGACA TCGGCCTCTT GCAGAACCTC CAGAACCTGG CTGTCACGGC CAACCGGATT 
GAGGCGCTGC CCCCGGAACT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC 
AACGTGCTGC AGTCCCTGCC CTCGAGGGTG GGTGAGCTGA CCAACCTAAC CCAGATTGAG 
CTGCGGGGCA ACCGGCTGGA GTGCCTGCCC GTGGAGCTGG GCGAGTGCCC ACTGCTCAAG 
CGCAGCGGCC TGGTGGTTGA GGAGGATCTG TTCAATACGT TGCCACCTGA GGTGAAGGAA 
CGGCTGTGGA GGGCCGACAA GGAGCAGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8A ( XM_005625263.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005625320.1 Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit A (LRRC8A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe12734
Clone ID Related Accession (Same CDS sequence)	XM_548430.4 , XM_548430.5 , XM_005625263.2 , XM_005625265.3 , XM_005625263.3 , XM_005625265.2 , XM_005625264.2 , XM_022423747.1
Accession Version	XM_005625263.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	volume-regulated anion channel subunit LRRC8A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005625263.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCCAG TGACAGAGCT CCGCTACTTT GCAGACACGC AGCCAGCGTA CCGGATCCTG 
AAGCCATGGT GGGACGTGTT CACAGACTAT ATCTCCATTG TGATGTTGAT GATCGCAGTC 
TTTGGGGGCA CGCTGCAGGT CACCCAGGAC AAGATGATCT GCCTGCCTTG TAAGTGGGTC 
ACCAAGGATT CCTGCAATGA CTCATTCCGA GGCTGGGCAG CCCCAGGCCC AGAGCCCACC 
TACCCAAACT CCACGGTCCC ACCGCCCCCT GGCACAGGCC CCACAGGCAT CAAGTACGAT 
CTCGACCGGC ACCAATACAA CTACGTGGAT GCTGTGTGCT ACGAGAACCG CCTGCACTGG 
TTTGCCAAGT ACTTCCCCTA TCTGGTGCTC CTGCACACGC TCATCTTCCT GGCCTGTAGC 
AACTTCTGGT TCAAGTTTCC GCGCACCAGC TCGAAGCTAG AGCACTTTGT GTCTATCCTG 
CTCAAGTGCT TTGACTCACC ATGGACTACG CGGGCCCTGT CAGAGACGGT GGTGGAAGAG 
AGCGACCCCA AGCCGGCCTT CAGCAAGATG AATGGCTCCA TGGACAAGAA GTCGTCGACG 
GTCAGTGAAG ATGTGGAGGC CACTGTGCCC ATGCTGCAGC GGACCAAGTC ACGGATCGAG 
CAGGGCATTG TGGACCGCTC AGAGACAGGT GTGCTGGACA AGAAAGAGGG GGAGCAGGCC 
AAGGCGCTGT TTGAGAAGGT CAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG 
TACCGCCTCT ATATGCGGCA GACCATCATC AAGGTGATTA AGTTCATGCT CATCATCTGC 
TACACTGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGTACAGT CGACATCGAG 
AGCCTGACGG GCTATCGCAC CTACCGCTGT GCCCACCCCC TGGCCACGCT CTTCAAGATC 
CTGGCATCCT TCTATATTAG CTTGGTCATC TTCTATGGGC TCATCTGCAT GTACACACTG 
TGGTGGATGT TGCGACGGTC CCTCAAGAAG TACTCATTTG AGTCGATCCG TGAGGAGAGC 
AGTTATAGTG ACATCCCCGA CGTCAAGAAT GACTTTGCCT TTATGCTCCA CCTCATCGAC 
CAGTACGACC CACTCTACTC AAAGCGCTTT GCTGTCTTCC TCTCGGAAGT GAGTGAGAAC 
AAGCTGCGGC AGCTGAACCT CAATAATGAG TGGACACTGG ACAAGCTGCG CCAGCGGCTC 
ACGAAGAATG CACAGGACAA GTTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC 
ACCGTGTTCG ATCTGGTGGA GCTAGAGGTG CTGAAGCTGG AGCTGATCCC AGACGTGACC 
ATTCCGCCCA GCATTGCGCA GCTCACAGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCA 
GCCAAGATTG AGGCACCTGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC CCTGCACATC 
AAGTTCACGG ACATCAAGGA GATCCCACTG TGGATCTACA GCCTGAAGAC GCTAGAGGAG 
CTGCACTTGA CGGGCAACCT GAGTGCTGAC AACAACCGCT ACATCGTCAT TGACGGGCTG 
CGTGAGCTCA AGCGCCTCAA GGTGCTGCGG CTCAAGAGCA ATCTGAGTAA GCTGCCACAA 
GTGGTCACCG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACCAAG 
CTCATCGTCC TCAATAGCCT CAAGAAGATG GTGAACCTGA CGGAGCTGGA GCTGATACGC 
TGCGACCTGG AGCGGATCCC GCACTCCATC TTTAGCCTCC ATAACCTGCA GGAGATCGAC 
CTCAAGGACA ACAACCTCAA GACCATCGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC 
CTCACCTGCC TTAAGCTGTG GTACAACCAC ATCGCCTACA TCCCCATCCA GATTGGCAAT 
CTCACCAACC TTGAGCGCCT CTACCTAAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG 
CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAATTT GACCTTCCTC 
CCTGCCGACA TCGGCCTCTT GCAGAACCTC CAGAACCTGG CTGTCACGGC CAACCGGATT 
GAGGCGCTGC CCCCGGAACT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC 
AACGTGCTGC AGTCCCTGCC CTCGAGGGTG GGTGAGCTGA CCAACCTAAC CCAGATTGAG 
CTGCGGGGCA ACCGGCTGGA GTGCCTGCCC GTGGAGCTGG GCGAGTGCCC ACTGCTCAAG 
CGCAGCGGCC TGGTGGTTGA GGAGGATCTG TTCAATACGT TGCCACCTGA GGTGAAGGAA 
CGGCTGTGGA GGGCCGACAA GGAGCAGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005625320.1
CDS	236..2668
Translation	MIPVTELRYFADTQPAYRILKPWWDVFTDYISIVMLMIAVFGGTLQVTQDKMICLPCKWVTKDSCNDSFRGWAAPGPEPTYPNSTVPPPPGTGPTGIKYDLDRHQYNYVDAVCYENRLHWFAKYFPYLVLLHTLIFLACSNFWFKFPRTSSKLEHFVSILLKCFDSPWTTRALSETVVEESDPKPAFSKMNGSMDKKSSTVSEDVEATVPMLQRTKSRIEQGIVDRSETGVLDKKEGEQAKALFEKVKKFRTHVEEGDIVYRLYMRQTIIKVIKFMLIICYTVYYVHNIKFDVDCTVDIESLTGYRTYRCAHPLATLFKILASFYISLVIFYGLICMYTLWWMLRRSLKKYSFESIREESSYSDIPDVKNDFAFMLHLIDQYDPLYSKRFAVFLSEVSENKLRQLNLNNEWTLDKLRQRLTKNAQDKLELHLFMLSGIPDTVFDLVELEVLKLELIPDVTIPPSIAQLTGLKELWLYHTAAKIEAPALAFLRENLRALHIKFTDIKEIPLWIYSLKTLEELHLTGNLSADNNRYIVIDGLRELKRLKVLRLKSNLSKLPQVVTDVGVHLQKLSINNEGTKLIVLNSLKKMVNLTELELIRCDLERIPHSIFSLHNLQEIDLKDNNLKTIEEIISFQHLHRLTCLKLWYNHIAYIPIQIGNLTNLERLYLNRNKIEKIPTQLFYCRKLRYLDLSHNNLTFLPADIGLLQNLQNLAVTANRIEALPPELFQCRKLRALHLGNNVLQSLPSRVGELTNLTQIELRGNRLECLPVELGECPLLKRSGLVVEEDLFNTLPPEVKERLWRADKEQA

Target ORF information:

RefSeq Version	XM_005625263.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit A (LRRC8A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005625263.3

ORF Insert Sequence:

ATGATTCCAG TGACAGAGCT CCGCTACTTT GCAGACACGC AGCCAGCGTA CCGGATCCTG 
AAGCCATGGT GGGACGTGTT CACAGACTAT ATCTCCATTG TGATGTTGAT GATCGCAGTC 
TTTGGGGGCA CGCTGCAGGT CACCCAGGAC AAGATGATCT GCCTGCCTTG TAAGTGGGTC 
ACCAAGGATT CCTGCAATGA CTCATTCCGA GGCTGGGCAG CCCCAGGCCC AGAGCCCACC 
TACCCAAACT CCACGGTCCC ACCGCCCCCT GGCACAGGCC CCACAGGCAT CAAGTACGAT 
CTCGACCGGC ACCAATACAA CTACGTGGAT GCTGTGTGCT ACGAGAACCG CCTGCACTGG 
TTTGCCAAGT ACTTCCCCTA TCTGGTGCTC CTGCACACGC TCATCTTCCT GGCCTGTAGC 
AACTTCTGGT TCAAGTTTCC GCGCACCAGC TCGAAGCTAG AGCACTTTGT GTCTATCCTG 
CTCAAGTGCT TTGACTCACC ATGGACTACG CGGGCCCTGT CAGAGACGGT GGTGGAAGAG 
AGCGACCCCA AGCCGGCCTT CAGCAAGATG AATGGCTCCA TGGACAAGAA GTCGTCGACG 
GTCAGTGAAG ATGTGGAGGC CACTGTGCCC ATGCTGCAGC GGACCAAGTC ACGGATCGAG 
CAGGGCATTG TGGACCGCTC AGAGACAGGT GTGCTGGACA AGAAAGAGGG GGAGCAGGCC 
AAGGCGCTGT TTGAGAAGGT CAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG 
TACCGCCTCT ATATGCGGCA GACCATCATC AAGGTGATTA AGTTCATGCT CATCATCTGC 
TACACTGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGTACAGT CGACATCGAG 
AGCCTGACGG GCTATCGCAC CTACCGCTGT GCCCACCCCC TGGCCACGCT CTTCAAGATC 
CTGGCATCCT TCTATATTAG CTTGGTCATC TTCTATGGGC TCATCTGCAT GTACACACTG 
TGGTGGATGT TGCGACGGTC CCTCAAGAAG TACTCATTTG AGTCGATCCG TGAGGAGAGC 
AGTTATAGTG ACATCCCCGA CGTCAAGAAT GACTTTGCCT TTATGCTCCA CCTCATCGAC 
CAGTACGACC CACTCTACTC AAAGCGCTTT GCTGTCTTCC TCTCGGAAGT GAGTGAGAAC 
AAGCTGCGGC AGCTGAACCT CAATAATGAG TGGACACTGG ACAAGCTGCG CCAGCGGCTC 
ACGAAGAATG CACAGGACAA GTTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC 
ACCGTGTTCG ATCTGGTGGA GCTAGAGGTG CTGAAGCTGG AGCTGATCCC AGACGTGACC 
ATTCCGCCCA GCATTGCGCA GCTCACAGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCA 
GCCAAGATTG AGGCACCTGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC CCTGCACATC 
AAGTTCACGG ACATCAAGGA GATCCCACTG TGGATCTACA GCCTGAAGAC GCTAGAGGAG 
CTGCACTTGA CGGGCAACCT GAGTGCTGAC AACAACCGCT ACATCGTCAT TGACGGGCTG 
CGTGAGCTCA AGCGCCTCAA GGTGCTGCGG CTCAAGAGCA ATCTGAGTAA GCTGCCACAA 
GTGGTCACCG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACCAAG 
CTCATCGTCC TCAATAGCCT CAAGAAGATG GTGAACCTGA CGGAGCTGGA GCTGATACGC 
TGCGACCTGG AGCGGATCCC GCACTCCATC TTTAGCCTCC ATAACCTGCA GGAGATCGAC 
CTCAAGGACA ACAACCTCAA GACCATCGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC 
CTCACCTGCC TTAAGCTGTG GTACAACCAC ATCGCCTACA TCCCCATCCA GATTGGCAAT 
CTCACCAACC TTGAGCGCCT CTACCTAAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG 
CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAATTT GACCTTCCTC 
CCTGCCGACA TCGGCCTCTT GCAGAACCTC CAGAACCTGG CTGTCACGGC CAACCGGATT 
GAGGCGCTGC CCCCGGAACT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC 
AACGTGCTGC AGTCCCTGCC CTCGAGGGTG GGTGAGCTGA CCAACCTAAC CCAGATTGAG 
CTGCGGGGCA ACCGGCTGGA GTGCCTGCCC GTGGAGCTGG GCGAGTGCCC ACTGCTCAAG 
CGCAGCGGCC TGGTGGTTGA GGAGGATCTG TTCAATACGT TGCCACCTGA GGTGAAGGAA 
CGGCTGTGGA GGGCCGACAA GGAGCAGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8A ( XM_005625265.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005625322.1 Canis lupus familiaris leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe12734
Clone ID Related Accession (Same CDS sequence)	XM_548430.4 , XM_548430.5 , XM_005625263.2 , XM_005625265.3 , XM_005625263.3 , XM_005625265.2 , XM_005625264.2 , XM_022423747.1
Accession Version	XM_005625265.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	volume-regulated anion channel subunit LRRC8A
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625265.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCCAG TGACAGAGCT CCGCTACTTT GCAGACACGC AGCCAGCGTA CCGGATCCTG 
AAGCCATGGT GGGACGTGTT CACAGACTAT ATCTCCATTG TGATGTTGAT GATCGCAGTC 
TTTGGGGGCA CGCTGCAGGT CACCCAGGAC AAGATGATCT GCCTGCCTTG TAAGTGGGTC 
ACCAAGGATT CCTGCAATGA CTCATTCCGA GGCTGGGCAG CCCCAGGCCC AGAGCCCACC 
TACCCAAACT CCACGGTCCC ACCGCCCCCT GGCACAGGCC CCACAGGCAT CAAGTACGAT 
CTCGACCGGC ACCAATACAA CTACGTGGAT GCTGTGTGCT ACGAGAACCG CCTGCACTGG 
TTTGCCAAGT ACTTCCCCTA TCTGGTGCTC CTGCACACGC TCATCTTCCT GGCCTGTAGC 
AACTTCTGGT TCAAGTTTCC GCGCACCAGC TCGAAGCTAG AGCACTTTGT GTCTATCCTG 
CTCAAGTGCT TTGACTCACC ATGGACTACG CGGGCCCTGT CAGAGACGGT GGTGGAAGAG 
AGCGACCCCA AGCCGGCCTT CAGCAAGATG AATGGCTCCA TGGACAAGAA GTCGTCGACG 
GTCAGTGAAG ATGTGGAGGC CACTGTGCCC ATGCTGCAGC GGACCAAGTC ACGGATCGAG 
CAGGGCATTG TGGACCGCTC AGAGACAGGT GTGCTGGACA AGAAAGAGGG GGAGCAGGCC 
AAGGCGCTGT TTGAGAAGGT CAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG 
TACCGCCTCT ATATGCGGCA GACCATCATC AAGGTGATTA AGTTCATGCT CATCATCTGC 
TACACTGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGTACAGT CGACATCGAG 
AGCCTGACGG GCTATCGCAC CTACCGCTGT GCCCACCCCC TGGCCACGCT CTTCAAGATC 
CTGGCATCCT TCTATATTAG CTTGGTCATC TTCTATGGGC TCATCTGCAT GTACACACTG 
TGGTGGATGT TGCGACGGTC CCTCAAGAAG TACTCATTTG AGTCGATCCG TGAGGAGAGC 
AGTTATAGTG ACATCCCCGA CGTCAAGAAT GACTTTGCCT TTATGCTCCA CCTCATCGAC 
CAGTACGACC CACTCTACTC AAAGCGCTTT GCTGTCTTCC TCTCGGAAGT GAGTGAGAAC 
AAGCTGCGGC AGCTGAACCT CAATAATGAG TGGACACTGG ACAAGCTGCG CCAGCGGCTC 
ACGAAGAATG CACAGGACAA GTTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC 
ACCGTGTTCG ATCTGGTGGA GCTAGAGGTG CTGAAGCTGG AGCTGATCCC AGACGTGACC 
ATTCCGCCCA GCATTGCGCA GCTCACAGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCA 
GCCAAGATTG AGGCACCTGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC CCTGCACATC 
AAGTTCACGG ACATCAAGGA GATCCCACTG TGGATCTACA GCCTGAAGAC GCTAGAGGAG 
CTGCACTTGA CGGGCAACCT GAGTGCTGAC AACAACCGCT ACATCGTCAT TGACGGGCTG 
CGTGAGCTCA AGCGCCTCAA GGTGCTGCGG CTCAAGAGCA ATCTGAGTAA GCTGCCACAA 
GTGGTCACCG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACCAAG 
CTCATCGTCC TCAATAGCCT CAAGAAGATG GTGAACCTGA CGGAGCTGGA GCTGATACGC 
TGCGACCTGG AGCGGATCCC GCACTCCATC TTTAGCCTCC ATAACCTGCA GGAGATCGAC 
CTCAAGGACA ACAACCTCAA GACCATCGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC 
CTCACCTGCC TTAAGCTGTG GTACAACCAC ATCGCCTACA TCCCCATCCA GATTGGCAAT 
CTCACCAACC TTGAGCGCCT CTACCTAAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG 
CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAATTT GACCTTCCTC 
CCTGCCGACA TCGGCCTCTT GCAGAACCTC CAGAACCTGG CTGTCACGGC CAACCGGATT 
GAGGCGCTGC CCCCGGAACT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC 
AACGTGCTGC AGTCCCTGCC CTCGAGGGTG GGTGAGCTGA CCAACCTAAC CCAGATTGAG 
CTGCGGGGCA ACCGGCTGGA GTGCCTGCCC GTGGAGCTGG GCGAGTGCCC ACTGCTCAAG 
CGCAGCGGCC TGGTGGTTGA GGAGGATCTG TTCAATACGT TGCCACCTGA GGTGAAGGAA 
CGGCTGTGGA GGGCCGACAA GGAGCAGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005625322.1
CDS	305..2737
Translation	MIPVTELRYFADTQPAYRILKPWWDVFTDYISIVMLMIAVFGGTLQVTQDKMICLPCKWVTKDSCNDSFRGWAAPGPEPTYPNSTVPPPPGTGPTGIKYDLDRHQYNYVDAVCYENRLHWFAKYFPYLVLLHTLIFLACSNFWFKFPRTSSKLEHFVSILLKCFDSPWTTRALSETVVEESDPKPAFSKMNGSMDKKSSTVSEDVEATVPMLQRTKSRIEQGIVDRSETGVLDKKEGEQAKALFEKVKKFRTHVEEGDIVYRLYMRQTIIKVIKFMLIICYTVYYVHNIKFDVDCTVDIESLTGYRTYRCAHPLATLFKILASFYISLVIFYGLICMYTLWWMLRRSLKKYSFESIREESSYSDIPDVKNDFAFMLHLIDQYDPLYSKRFAVFLSEVSENKLRQLNLNNEWTLDKLRQRLTKNAQDKLELHLFMLSGIPDTVFDLVELEVLKLELIPDVTIPPSIAQLTGLKELWLYHTAAKIEAPALAFLRENLRALHIKFTDIKEIPLWIYSLKTLEELHLTGNLSADNNRYIVIDGLRELKRLKVLRLKSNLSKLPQVVTDVGVHLQKLSINNEGTKLIVLNSLKKMVNLTELELIRCDLERIPHSIFSLHNLQEIDLKDNNLKTIEEIISFQHLHRLTCLKLWYNHIAYIPIQIGNLTNLERLYLNRNKIEKIPTQLFYCRKLRYLDLSHNNLTFLPADIGLLQNLQNLAVTANRIEALPPELFQCRKLRALHLGNNVLQSLPSRVGELTNLTQIELRGNRLECLPVELGECPLLKRSGLVVEEDLFNTLPPEVKERLWRADKEQA

Target ORF information:

RefSeq Version	XM_005625265.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005625265.2

ORF Insert Sequence:

ATGATTCCAG TGACAGAGCT CCGCTACTTT GCAGACACGC AGCCAGCGTA CCGGATCCTG 
AAGCCATGGT GGGACGTGTT CACAGACTAT ATCTCCATTG TGATGTTGAT GATCGCAGTC 
TTTGGGGGCA CGCTGCAGGT CACCCAGGAC AAGATGATCT GCCTGCCTTG TAAGTGGGTC 
ACCAAGGATT CCTGCAATGA CTCATTCCGA GGCTGGGCAG CCCCAGGCCC AGAGCCCACC 
TACCCAAACT CCACGGTCCC ACCGCCCCCT GGCACAGGCC CCACAGGCAT CAAGTACGAT 
CTCGACCGGC ACCAATACAA CTACGTGGAT GCTGTGTGCT ACGAGAACCG CCTGCACTGG 
TTTGCCAAGT ACTTCCCCTA TCTGGTGCTC CTGCACACGC TCATCTTCCT GGCCTGTAGC 
AACTTCTGGT TCAAGTTTCC GCGCACCAGC TCGAAGCTAG AGCACTTTGT GTCTATCCTG 
CTCAAGTGCT TTGACTCACC ATGGACTACG CGGGCCCTGT CAGAGACGGT GGTGGAAGAG 
AGCGACCCCA AGCCGGCCTT CAGCAAGATG AATGGCTCCA TGGACAAGAA GTCGTCGACG 
GTCAGTGAAG ATGTGGAGGC CACTGTGCCC ATGCTGCAGC GGACCAAGTC ACGGATCGAG 
CAGGGCATTG TGGACCGCTC AGAGACAGGT GTGCTGGACA AGAAAGAGGG GGAGCAGGCC 
AAGGCGCTGT TTGAGAAGGT CAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG 
TACCGCCTCT ATATGCGGCA GACCATCATC AAGGTGATTA AGTTCATGCT CATCATCTGC 
TACACTGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGTACAGT CGACATCGAG 
AGCCTGACGG GCTATCGCAC CTACCGCTGT GCCCACCCCC TGGCCACGCT CTTCAAGATC 
CTGGCATCCT TCTATATTAG CTTGGTCATC TTCTATGGGC TCATCTGCAT GTACACACTG 
TGGTGGATGT TGCGACGGTC CCTCAAGAAG TACTCATTTG AGTCGATCCG TGAGGAGAGC 
AGTTATAGTG ACATCCCCGA CGTCAAGAAT GACTTTGCCT TTATGCTCCA CCTCATCGAC 
CAGTACGACC CACTCTACTC AAAGCGCTTT GCTGTCTTCC TCTCGGAAGT GAGTGAGAAC 
AAGCTGCGGC AGCTGAACCT CAATAATGAG TGGACACTGG ACAAGCTGCG CCAGCGGCTC 
ACGAAGAATG CACAGGACAA GTTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC 
ACCGTGTTCG ATCTGGTGGA GCTAGAGGTG CTGAAGCTGG AGCTGATCCC AGACGTGACC 
ATTCCGCCCA GCATTGCGCA GCTCACAGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCA 
GCCAAGATTG AGGCACCTGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC CCTGCACATC 
AAGTTCACGG ACATCAAGGA GATCCCACTG TGGATCTACA GCCTGAAGAC GCTAGAGGAG 
CTGCACTTGA CGGGCAACCT GAGTGCTGAC AACAACCGCT ACATCGTCAT TGACGGGCTG 
CGTGAGCTCA AGCGCCTCAA GGTGCTGCGG CTCAAGAGCA ATCTGAGTAA GCTGCCACAA 
GTGGTCACCG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACCAAG 
CTCATCGTCC TCAATAGCCT CAAGAAGATG GTGAACCTGA CGGAGCTGGA GCTGATACGC 
TGCGACCTGG AGCGGATCCC GCACTCCATC TTTAGCCTCC ATAACCTGCA GGAGATCGAC 
CTCAAGGACA ACAACCTCAA GACCATCGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC 
CTCACCTGCC TTAAGCTGTG GTACAACCAC ATCGCCTACA TCCCCATCCA GATTGGCAAT 
CTCACCAACC TTGAGCGCCT CTACCTAAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG 
CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAATTT GACCTTCCTC 
CCTGCCGACA TCGGCCTCTT GCAGAACCTC CAGAACCTGG CTGTCACGGC CAACCGGATT 
GAGGCGCTGC CCCCGGAACT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC 
AACGTGCTGC AGTCCCTGCC CTCGAGGGTG GGTGAGCTGA CCAACCTAAC CCAGATTGAG 
CTGCGGGGCA ACCGGCTGGA GTGCCTGCCC GTGGAGCTGG GCGAGTGCCC ACTGCTCAAG 
CGCAGCGGCC TGGTGGTTGA GGAGGATCTG TTCAATACGT TGCCACCTGA GGTGAAGGAA 
CGGCTGTGGA GGGCCGACAA GGAGCAGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8A ( XM_005625264.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005625321.1 Canis lupus familiaris leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe12734
Clone ID Related Accession (Same CDS sequence)	XM_548430.4 , XM_548430.5 , XM_005625263.2 , XM_005625265.3 , XM_005625263.3 , XM_005625265.2 , XM_005625264.2 , XM_022423747.1
Accession Version	XM_005625264.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	volume-regulated anion channel subunit LRRC8A
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625264.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCCAG TGACAGAGCT CCGCTACTTT GCAGACACGC AGCCAGCGTA CCGGATCCTG 
AAGCCATGGT GGGACGTGTT CACAGACTAT ATCTCCATTG TGATGTTGAT GATCGCAGTC 
TTTGGGGGCA CGCTGCAGGT CACCCAGGAC AAGATGATCT GCCTGCCTTG TAAGTGGGTC 
ACCAAGGATT CCTGCAATGA CTCATTCCGA GGCTGGGCAG CCCCAGGCCC AGAGCCCACC 
TACCCAAACT CCACGGTCCC ACCGCCCCCT GGCACAGGCC CCACAGGCAT CAAGTACGAT 
CTCGACCGGC ACCAATACAA CTACGTGGAT GCTGTGTGCT ACGAGAACCG CCTGCACTGG 
TTTGCCAAGT ACTTCCCCTA TCTGGTGCTC CTGCACACGC TCATCTTCCT GGCCTGTAGC 
AACTTCTGGT TCAAGTTTCC GCGCACCAGC TCGAAGCTAG AGCACTTTGT GTCTATCCTG 
CTCAAGTGCT TTGACTCACC ATGGACTACG CGGGCCCTGT CAGAGACGGT GGTGGAAGAG 
AGCGACCCCA AGCCGGCCTT CAGCAAGATG AATGGCTCCA TGGACAAGAA GTCGTCGACG 
GTCAGTGAAG ATGTGGAGGC CACTGTGCCC ATGCTGCAGC GGACCAAGTC ACGGATCGAG 
CAGGGCATTG TGGACCGCTC AGAGACAGGT GTGCTGGACA AGAAAGAGGG GGAGCAGGCC 
AAGGCGCTGT TTGAGAAGGT CAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG 
TACCGCCTCT ATATGCGGCA GACCATCATC AAGGTGATTA AGTTCATGCT CATCATCTGC 
TACACTGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGTACAGT CGACATCGAG 
AGCCTGACGG GCTATCGCAC CTACCGCTGT GCCCACCCCC TGGCCACGCT CTTCAAGATC 
CTGGCATCCT TCTATATTAG CTTGGTCATC TTCTATGGGC TCATCTGCAT GTACACACTG 
TGGTGGATGT TGCGACGGTC CCTCAAGAAG TACTCATTTG AGTCGATCCG TGAGGAGAGC 
AGTTATAGTG ACATCCCCGA CGTCAAGAAT GACTTTGCCT TTATGCTCCA CCTCATCGAC 
CAGTACGACC CACTCTACTC AAAGCGCTTT GCTGTCTTCC TCTCGGAAGT GAGTGAGAAC 
AAGCTGCGGC AGCTGAACCT CAATAATGAG TGGACACTGG ACAAGCTGCG CCAGCGGCTC 
ACGAAGAATG CACAGGACAA GTTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC 
ACCGTGTTCG ATCTGGTGGA GCTAGAGGTG CTGAAGCTGG AGCTGATCCC AGACGTGACC 
ATTCCGCCCA GCATTGCGCA GCTCACAGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCA 
GCCAAGATTG AGGCACCTGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC CCTGCACATC 
AAGTTCACGG ACATCAAGGA GATCCCACTG TGGATCTACA GCCTGAAGAC GCTAGAGGAG 
CTGCACTTGA CGGGCAACCT GAGTGCTGAC AACAACCGCT ACATCGTCAT TGACGGGCTG 
CGTGAGCTCA AGCGCCTCAA GGTGCTGCGG CTCAAGAGCA ATCTGAGTAA GCTGCCACAA 
GTGGTCACCG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACCAAG 
CTCATCGTCC TCAATAGCCT CAAGAAGATG GTGAACCTGA CGGAGCTGGA GCTGATACGC 
TGCGACCTGG AGCGGATCCC GCACTCCATC TTTAGCCTCC ATAACCTGCA GGAGATCGAC 
CTCAAGGACA ACAACCTCAA GACCATCGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC 
CTCACCTGCC TTAAGCTGTG GTACAACCAC ATCGCCTACA TCCCCATCCA GATTGGCAAT 
CTCACCAACC TTGAGCGCCT CTACCTAAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG 
CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAATTT GACCTTCCTC 
CCTGCCGACA TCGGCCTCTT GCAGAACCTC CAGAACCTGG CTGTCACGGC CAACCGGATT 
GAGGCGCTGC CCCCGGAACT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC 
AACGTGCTGC AGTCCCTGCC CTCGAGGGTG GGTGAGCTGA CCAACCTAAC CCAGATTGAG 
CTGCGGGGCA ACCGGCTGGA GTGCCTGCCC GTGGAGCTGG GCGAGTGCCC ACTGCTCAAG 
CGCAGCGGCC TGGTGGTTGA GGAGGATCTG TTCAATACGT TGCCACCTGA GGTGAAGGAA 
CGGCTGTGGA GGGCCGACAA GGAGCAGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005625321.1
CDS	203..2635
Translation	MIPVTELRYFADTQPAYRILKPWWDVFTDYISIVMLMIAVFGGTLQVTQDKMICLPCKWVTKDSCNDSFRGWAAPGPEPTYPNSTVPPPPGTGPTGIKYDLDRHQYNYVDAVCYENRLHWFAKYFPYLVLLHTLIFLACSNFWFKFPRTSSKLEHFVSILLKCFDSPWTTRALSETVVEESDPKPAFSKMNGSMDKKSSTVSEDVEATVPMLQRTKSRIEQGIVDRSETGVLDKKEGEQAKALFEKVKKFRTHVEEGDIVYRLYMRQTIIKVIKFMLIICYTVYYVHNIKFDVDCTVDIESLTGYRTYRCAHPLATLFKILASFYISLVIFYGLICMYTLWWMLRRSLKKYSFESIREESSYSDIPDVKNDFAFMLHLIDQYDPLYSKRFAVFLSEVSENKLRQLNLNNEWTLDKLRQRLTKNAQDKLELHLFMLSGIPDTVFDLVELEVLKLELIPDVTIPPSIAQLTGLKELWLYHTAAKIEAPALAFLRENLRALHIKFTDIKEIPLWIYSLKTLEELHLTGNLSADNNRYIVIDGLRELKRLKVLRLKSNLSKLPQVVTDVGVHLQKLSINNEGTKLIVLNSLKKMVNLTELELIRCDLERIPHSIFSLHNLQEIDLKDNNLKTIEEIISFQHLHRLTCLKLWYNHIAYIPIQIGNLTNLERLYLNRNKIEKIPTQLFYCRKLRYLDLSHNNLTFLPADIGLLQNLQNLAVTANRIEALPPELFQCRKLRALHLGNNVLQSLPSRVGELTNLTQIELRGNRLECLPVELGECPLLKRSGLVVEEDLFNTLPPEVKERLWRADKEQA

Target ORF information:

RefSeq Version	XM_005625264.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005625264.2

ORF Insert Sequence:

ATGATTCCAG TGACAGAGCT CCGCTACTTT GCAGACACGC AGCCAGCGTA CCGGATCCTG 
AAGCCATGGT GGGACGTGTT CACAGACTAT ATCTCCATTG TGATGTTGAT GATCGCAGTC 
TTTGGGGGCA CGCTGCAGGT CACCCAGGAC AAGATGATCT GCCTGCCTTG TAAGTGGGTC 
ACCAAGGATT CCTGCAATGA CTCATTCCGA GGCTGGGCAG CCCCAGGCCC AGAGCCCACC 
TACCCAAACT CCACGGTCCC ACCGCCCCCT GGCACAGGCC CCACAGGCAT CAAGTACGAT 
CTCGACCGGC ACCAATACAA CTACGTGGAT GCTGTGTGCT ACGAGAACCG CCTGCACTGG 
TTTGCCAAGT ACTTCCCCTA TCTGGTGCTC CTGCACACGC TCATCTTCCT GGCCTGTAGC 
AACTTCTGGT TCAAGTTTCC GCGCACCAGC TCGAAGCTAG AGCACTTTGT GTCTATCCTG 
CTCAAGTGCT TTGACTCACC ATGGACTACG CGGGCCCTGT CAGAGACGGT GGTGGAAGAG 
AGCGACCCCA AGCCGGCCTT CAGCAAGATG AATGGCTCCA TGGACAAGAA GTCGTCGACG 
GTCAGTGAAG ATGTGGAGGC CACTGTGCCC ATGCTGCAGC GGACCAAGTC ACGGATCGAG 
CAGGGCATTG TGGACCGCTC AGAGACAGGT GTGCTGGACA AGAAAGAGGG GGAGCAGGCC 
AAGGCGCTGT TTGAGAAGGT CAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG 
TACCGCCTCT ATATGCGGCA GACCATCATC AAGGTGATTA AGTTCATGCT CATCATCTGC 
TACACTGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGTACAGT CGACATCGAG 
AGCCTGACGG GCTATCGCAC CTACCGCTGT GCCCACCCCC TGGCCACGCT CTTCAAGATC 
CTGGCATCCT TCTATATTAG CTTGGTCATC TTCTATGGGC TCATCTGCAT GTACACACTG 
TGGTGGATGT TGCGACGGTC CCTCAAGAAG TACTCATTTG AGTCGATCCG TGAGGAGAGC 
AGTTATAGTG ACATCCCCGA CGTCAAGAAT GACTTTGCCT TTATGCTCCA CCTCATCGAC 
CAGTACGACC CACTCTACTC AAAGCGCTTT GCTGTCTTCC TCTCGGAAGT GAGTGAGAAC 
AAGCTGCGGC AGCTGAACCT CAATAATGAG TGGACACTGG ACAAGCTGCG CCAGCGGCTC 
ACGAAGAATG CACAGGACAA GTTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC 
ACCGTGTTCG ATCTGGTGGA GCTAGAGGTG CTGAAGCTGG AGCTGATCCC AGACGTGACC 
ATTCCGCCCA GCATTGCGCA GCTCACAGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCA 
GCCAAGATTG AGGCACCTGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC CCTGCACATC 
AAGTTCACGG ACATCAAGGA GATCCCACTG TGGATCTACA GCCTGAAGAC GCTAGAGGAG 
CTGCACTTGA CGGGCAACCT GAGTGCTGAC AACAACCGCT ACATCGTCAT TGACGGGCTG 
CGTGAGCTCA AGCGCCTCAA GGTGCTGCGG CTCAAGAGCA ATCTGAGTAA GCTGCCACAA 
GTGGTCACCG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACCAAG 
CTCATCGTCC TCAATAGCCT CAAGAAGATG GTGAACCTGA CGGAGCTGGA GCTGATACGC 
TGCGACCTGG AGCGGATCCC GCACTCCATC TTTAGCCTCC ATAACCTGCA GGAGATCGAC 
CTCAAGGACA ACAACCTCAA GACCATCGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC 
CTCACCTGCC TTAAGCTGTG GTACAACCAC ATCGCCTACA TCCCCATCCA GATTGGCAAT 
CTCACCAACC TTGAGCGCCT CTACCTAAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG 
CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAATTT GACCTTCCTC 
CCTGCCGACA TCGGCCTCTT GCAGAACCTC CAGAACCTGG CTGTCACGGC CAACCGGATT 
GAGGCGCTGC CCCCGGAACT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC 
AACGTGCTGC AGTCCCTGCC CTCGAGGGTG GGTGAGCTGA CCAACCTAAC CCAGATTGAG 
CTGCGGGGCA ACCGGCTGGA GTGCCTGCCC GTGGAGCTGG GCGAGTGCCC ACTGCTCAAG 
CGCAGCGGCC TGGTGGTTGA GGAGGATCTG TTCAATACGT TGCCACCTGA GGTGAAGGAA 
CGGCTGTGGA GGGCCGACAA GGAGCAGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8A ( XM_022423747.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022279455.1 Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit A (LRRC8A), transcript variant X3, mRNA.

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CloneID	OCe12734
Clone ID Related Accession (Same CDS sequence)	XM_548430.4 , XM_548430.5 , XM_005625263.2 , XM_005625265.3 , XM_005625263.3 , XM_005625265.2 , XM_005625264.2 , XM_022423747.1
Accession Version	XM_022423747.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	volume-regulated anion channel subunit LRRC8A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCCAG TGACAGAGCT CCGCTACTTT GCAGACACGC AGCCAGCGTA CCGGATCCTG 
AAGCCATGGT GGGACGTGTT CACAGACTAT ATCTCCATTG TGATGTTGAT GATCGCAGTC 
TTTGGGGGCA CGCTGCAGGT CACCCAGGAC AAGATGATCT GCCTGCCTTG TAAGTGGGTC 
ACCAAGGATT CCTGCAATGA CTCATTCCGA GGCTGGGCAG CCCCAGGCCC AGAGCCCACC 
TACCCAAACT CCACGGTCCC ACCGCCCCCT GGCACAGGCC CCACAGGCAT CAAGTACGAT 
CTCGACCGGC ACCAATACAA CTACGTGGAT GCTGTGTGCT ACGAGAACCG CCTGCACTGG 
TTTGCCAAGT ACTTCCCCTA TCTGGTGCTC CTGCACACGC TCATCTTCCT GGCCTGTAGC 
AACTTCTGGT TCAAGTTTCC GCGCACCAGC TCGAAGCTAG AGCACTTTGT GTCTATCCTG 
CTCAAGTGCT TTGACTCACC ATGGACTACG CGGGCCCTGT CAGAGACGGT GGTGGAAGAG 
AGCGACCCCA AGCCGGCCTT CAGCAAGATG AATGGCTCCA TGGACAAGAA GTCGTCGACG 
GTCAGTGAAG ATGTGGAGGC CACTGTGCCC ATGCTGCAGC GGACCAAGTC ACGGATCGAG 
CAGGGCATTG TGGACCGCTC AGAGACAGGT GTGCTGGACA AGAAAGAGGG GGAGCAGGCC 
AAGGCGCTGT TTGAGAAGGT CAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG 
TACCGCCTCT ATATGCGGCA GACCATCATC AAGGTGATTA AGTTCATGCT CATCATCTGC 
TACACTGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGTACAGT CGACATCGAG 
AGCCTGACGG GCTATCGCAC CTACCGCTGT GCCCACCCCC TGGCCACGCT CTTCAAGATC 
CTGGCATCCT TCTATATTAG CTTGGTCATC TTCTATGGGC TCATCTGCAT GTACACACTG 
TGGTGGATGT TGCGACGGTC CCTCAAGAAG TACTCATTTG AGTCGATCCG TGAGGAGAGC 
AGTTATAGTG ACATCCCCGA CGTCAAGAAT GACTTTGCCT TTATGCTCCA CCTCATCGAC 
CAGTACGACC CACTCTACTC AAAGCGCTTT GCTGTCTTCC TCTCGGAAGT GAGTGAGAAC 
AAGCTGCGGC AGCTGAACCT CAATAATGAG TGGACACTGG ACAAGCTGCG CCAGCGGCTC 
ACGAAGAATG CACAGGACAA GTTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC 
ACCGTGTTCG ATCTGGTGGA GCTAGAGGTG CTGAAGCTGG AGCTGATCCC AGACGTGACC 
ATTCCGCCCA GCATTGCGCA GCTCACAGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCA 
GCCAAGATTG AGGCACCTGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC CCTGCACATC 
AAGTTCACGG ACATCAAGGA GATCCCACTG TGGATCTACA GCCTGAAGAC GCTAGAGGAG 
CTGCACTTGA CGGGCAACCT GAGTGCTGAC AACAACCGCT ACATCGTCAT TGACGGGCTG 
CGTGAGCTCA AGCGCCTCAA GGTGCTGCGG CTCAAGAGCA ATCTGAGTAA GCTGCCACAA 
GTGGTCACCG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACCAAG 
CTCATCGTCC TCAATAGCCT CAAGAAGATG GTGAACCTGA CGGAGCTGGA GCTGATACGC 
TGCGACCTGG AGCGGATCCC GCACTCCATC TTTAGCCTCC ATAACCTGCA GGAGATCGAC 
CTCAAGGACA ACAACCTCAA GACCATCGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC 
CTCACCTGCC TTAAGCTGTG GTACAACCAC ATCGCCTACA TCCCCATCCA GATTGGCAAT 
CTCACCAACC TTGAGCGCCT CTACCTAAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG 
CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAATTT GACCTTCCTC 
CCTGCCGACA TCGGCCTCTT GCAGAACCTC CAGAACCTGG CTGTCACGGC CAACCGGATT 
GAGGCGCTGC CCCCGGAACT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC 
AACGTGCTGC AGTCCCTGCC CTCGAGGGTG GGTGAGCTGA CCAACCTAAC CCAGATTGAG 
CTGCGGGGCA ACCGGCTGGA GTGCCTGCCC GTGGAGCTGG GCGAGTGCCC ACTGCTCAAG 
CGCAGCGGCC TGGTGGTTGA GGAGGATCTG TTCAATACGT TGCCACCTGA GGTGAAGGAA 
CGGCTGTGGA GGGCCGACAA GGAGCAGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022279455.1
CDS	96..2528
Translation	MIPVTELRYFADTQPAYRILKPWWDVFTDYISIVMLMIAVFGGTLQVTQDKMICLPCKWVTKDSCNDSFRGWAAPGPEPTYPNSTVPPPPGTGPTGIKYDLDRHQYNYVDAVCYENRLHWFAKYFPYLVLLHTLIFLACSNFWFKFPRTSSKLEHFVSILLKCFDSPWTTRALSETVVEESDPKPAFSKMNGSMDKKSSTVSEDVEATVPMLQRTKSRIEQGIVDRSETGVLDKKEGEQAKALFEKVKKFRTHVEEGDIVYRLYMRQTIIKVIKFMLIICYTVYYVHNIKFDVDCTVDIESLTGYRTYRCAHPLATLFKILASFYISLVIFYGLICMYTLWWMLRRSLKKYSFESIREESSYSDIPDVKNDFAFMLHLIDQYDPLYSKRFAVFLSEVSENKLRQLNLNNEWTLDKLRQRLTKNAQDKLELHLFMLSGIPDTVFDLVELEVLKLELIPDVTIPPSIAQLTGLKELWLYHTAAKIEAPALAFLRENLRALHIKFTDIKEIPLWIYSLKTLEELHLTGNLSADNNRYIVIDGLRELKRLKVLRLKSNLSKLPQVVTDVGVHLQKLSINNEGTKLIVLNSLKKMVNLTELELIRCDLERIPHSIFSLHNLQEIDLKDNNLKTIEEIISFQHLHRLTCLKLWYNHIAYIPIQIGNLTNLERLYLNRNKIEKIPTQLFYCRKLRYLDLSHNNLTFLPADIGLLQNLQNLAVTANRIEALPPELFQCRKLRALHLGNNVLQSLPSRVGELTNLTQIELRGNRLECLPVELGECPLLKRSGLVVEEDLFNTLPPEVKERLWRADKEQA

Target ORF information:

RefSeq Version	XM_022423747.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit A (LRRC8A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022423747.1

ORF Insert Sequence:

ATGATTCCAG TGACAGAGCT CCGCTACTTT GCAGACACGC AGCCAGCGTA CCGGATCCTG 
AAGCCATGGT GGGACGTGTT CACAGACTAT ATCTCCATTG TGATGTTGAT GATCGCAGTC 
TTTGGGGGCA CGCTGCAGGT CACCCAGGAC AAGATGATCT GCCTGCCTTG TAAGTGGGTC 
ACCAAGGATT CCTGCAATGA CTCATTCCGA GGCTGGGCAG CCCCAGGCCC AGAGCCCACC 
TACCCAAACT CCACGGTCCC ACCGCCCCCT GGCACAGGCC CCACAGGCAT CAAGTACGAT 
CTCGACCGGC ACCAATACAA CTACGTGGAT GCTGTGTGCT ACGAGAACCG CCTGCACTGG 
TTTGCCAAGT ACTTCCCCTA TCTGGTGCTC CTGCACACGC TCATCTTCCT GGCCTGTAGC 
AACTTCTGGT TCAAGTTTCC GCGCACCAGC TCGAAGCTAG AGCACTTTGT GTCTATCCTG 
CTCAAGTGCT TTGACTCACC ATGGACTACG CGGGCCCTGT CAGAGACGGT GGTGGAAGAG 
AGCGACCCCA AGCCGGCCTT CAGCAAGATG AATGGCTCCA TGGACAAGAA GTCGTCGACG 
GTCAGTGAAG ATGTGGAGGC CACTGTGCCC ATGCTGCAGC GGACCAAGTC ACGGATCGAG 
CAGGGCATTG TGGACCGCTC AGAGACAGGT GTGCTGGACA AGAAAGAGGG GGAGCAGGCC 
AAGGCGCTGT TTGAGAAGGT CAAGAAGTTC CGGACCCACG TGGAGGAGGG GGACATCGTG 
TACCGCCTCT ATATGCGGCA GACCATCATC AAGGTGATTA AGTTCATGCT CATCATCTGC 
TACACTGTCT ACTACGTGCA CAACATCAAG TTCGACGTGG ACTGTACAGT CGACATCGAG 
AGCCTGACGG GCTATCGCAC CTACCGCTGT GCCCACCCCC TGGCCACGCT CTTCAAGATC 
CTGGCATCCT TCTATATTAG CTTGGTCATC TTCTATGGGC TCATCTGCAT GTACACACTG 
TGGTGGATGT TGCGACGGTC CCTCAAGAAG TACTCATTTG AGTCGATCCG TGAGGAGAGC 
AGTTATAGTG ACATCCCCGA CGTCAAGAAT GACTTTGCCT TTATGCTCCA CCTCATCGAC 
CAGTACGACC CACTCTACTC AAAGCGCTTT GCTGTCTTCC TCTCGGAAGT GAGTGAGAAC 
AAGCTGCGGC AGCTGAACCT CAATAATGAG TGGACACTGG ACAAGCTGCG CCAGCGGCTC 
ACGAAGAATG CACAGGACAA GTTGGAGCTG CACCTGTTCA TGCTCAGCGG CATCCCTGAC 
ACCGTGTTCG ATCTGGTGGA GCTAGAGGTG CTGAAGCTGG AGCTGATCCC AGACGTGACC 
ATTCCGCCCA GCATTGCGCA GCTCACAGGC CTCAAGGAGC TGTGGCTGTA CCACACGGCA 
GCCAAGATTG AGGCACCTGC CCTGGCCTTC CTGCGCGAGA ACCTGCGGGC CCTGCACATC 
AAGTTCACGG ACATCAAGGA GATCCCACTG TGGATCTACA GCCTGAAGAC GCTAGAGGAG 
CTGCACTTGA CGGGCAACCT GAGTGCTGAC AACAACCGCT ACATCGTCAT TGACGGGCTG 
CGTGAGCTCA AGCGCCTCAA GGTGCTGCGG CTCAAGAGCA ATCTGAGTAA GCTGCCACAA 
GTGGTCACCG ACGTGGGCGT GCACCTGCAG AAACTGTCCA TCAACAATGA GGGCACCAAG 
CTCATCGTCC TCAATAGCCT CAAGAAGATG GTGAACCTGA CGGAGCTGGA GCTGATACGC 
TGCGACCTGG AGCGGATCCC GCACTCCATC TTTAGCCTCC ATAACCTGCA GGAGATCGAC 
CTCAAGGACA ACAACCTCAA GACCATCGAG GAGATCATCA GCTTCCAGCA CCTGCACCGC 
CTCACCTGCC TTAAGCTGTG GTACAACCAC ATCGCCTACA TCCCCATCCA GATTGGCAAT 
CTCACCAACC TTGAGCGCCT CTACCTAAAC CGCAACAAGA TCGAGAAGAT CCCCACCCAG 
CTCTTCTACT GCCGCAAGCT GCGCTACCTG GACCTCAGCC ACAACAATTT GACCTTCCTC 
CCTGCCGACA TCGGCCTCTT GCAGAACCTC CAGAACCTGG CTGTCACGGC CAACCGGATT 
GAGGCGCTGC CCCCGGAACT CTTCCAGTGC CGGAAGCTGC GGGCCCTGCA CCTGGGCAAC 
AACGTGCTGC AGTCCCTGCC CTCGAGGGTG GGTGAGCTGA CCAACCTAAC CCAGATTGAG 
CTGCGGGGCA ACCGGCTGGA GTGCCTGCCC GTGGAGCTGG GCGAGTGCCC ACTGCTCAAG 
CGCAGCGGCC TGGTGGTTGA GGAGGATCTG TTCAATACGT TGCCACCTGA GGTGAAGGAA 
CGGCTGTGGA GGGCCGACAA GGAGCAGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LRRC8A cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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