MTCL1 cDNA ORF clone, Canis lupus familiaris(dog)

The following MTCL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTCL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe09959 XM_022421620.1
Latest version!
Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe289879 XM_022421619.1
Latest version!
Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe289880 XM_022421621.1
Latest version!
Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe289881 XM_022421622.1
Latest version!
Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe289882 XM_022421623.1
Latest version!
Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe289883 XM_022421624.1
Latest version!
Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe289884 XM_022421625.1
Latest version!
Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe289885 XM_022421626.1
Latest version!
Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe289886 XM_022421627.1
Latest version!
Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe289887 XM_022421628.1
Latest version!
Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe289888 XM_022421629.1
Latest version!
Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe289889 XM_022421630.1
Latest version!
Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe289890 XM_022421631.1
Latest version!
Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe09960 XM_005623075.1
Latest version!
Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe09963 XM_005623078.1
Latest version!
Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe09965 XM_005623080.3
Latest version!
Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OCe09965 XM_005623080.2 Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe09959
    Clone ID Related Accession (Same CDS sequence) XM_022421620.1
    Accession Version XM_022421620.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product microtubule cross-linking factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    CAGAGTGGTG GAACCGAGCG CCTTGGAAAC TGCAGGTCTA CAACCAAAAC TCAAAGGAAA
    CTGGCACCCC GGCGCAAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA
    GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG
    GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG
    GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG
    GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA
    GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG
    GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG
    CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT
    GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT
    CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT
    CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG
    AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC
    CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG
    AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC
    GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA
    TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC
    CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG
    GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG
    CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG
    GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG
    GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC
    CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC
    GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGTCCA AACTGAGAGG TCAGCTGGAG
    TGGCAGCCGG GGCAGGAGTG CGGGGACGAG CCAGAGCAGC TGCACCGCCG CGCTGATGGG
    GACGTCGGGA GCTGCCGGCA GGGAGGCCAG AGCTGCTTCA GTCTAGAGAG TGTATCGCTA
    GAGGAGCTGC AGGGTCAGCT TGCGCAGGCG ACCAGACTGC ATCAAGAGGA GACAGAGAAA
    TTTACAAACA AGATTCGGAA GATGGAGGAG GAGCACCTGT ACGCCTTGAG GTGGAAAGAA
    CTGGAAATGC ACAGCCTGGC TTTGCAGAAC ACCCTCCATG AGCAAACCTG GAGTGATGAG
    AGGAACCTGA TGCAGCAGGA GCTCCGGTCT ATGAAGCAGA ACATTTTCCT TTTCTACGTC
    AAACTCAGAT GGCTGCTGAG GCACTGGCGA CAAGGGAAGC AGATGGAGGA AGAAGGCGAG
    GATTTCACTG AGAGCGAGCA TCCAGAGACC CTCCCCAGGC TTGGTGAGCT TGGAGTGCAG
    GGGGACCCGA AGGGGGACGG CCCAGACCAA GATGACAACG GTCCGGGCTG CGGCTTTCCG
    CTGGGAGAGC ATCCTCCACA CTCCCCTGTG CAGATTGGTG ACCATGGATC GAGACTACAG
    CCTACACATG GGAGACAGCT CCACAGGCAG GTGGTGGAAA ACCAGCAGCT GTTCAGTGCA
    CTCAAGAGCC TGCTGGAGGA CCTGCGCGCC GAGCTGCGGG AGGACGAGCA TGCACGGCGG
    CGGCTGCAGC AGCAGTACGC CAGCGACAAA GCCGCCTGGG ACGTGGAGTG GGCTGCACTT
    ACCTGCCGCC TGGAACAGCT GGAAGAGAAG ACTGAGAAGA ATTTGGGAGA AGCATGCTCT
    TCCACCGACA GCAAAGGGGC ATTCAAGAAG GAAAGAGAGA TGCATCAGAA GCTCCTCACT
    GACAGTCATG GCCTGGTCAT GGACCTGCGC TGGCAGATCC ATCACAGTGA GAAGAACTGG
    AGCCGGGAGA AGGTGGAACT TCTTAGCCGT CTAGACAGAG ACCGGCAGGA ATGGGAGCAG
    CAGAAGAAGG AGCTCTTGTG GAGAATAGAA CAGTTGCAGA AAGAAAACAG TCCCCGGAGA
    AGTGGCAGTT TCCTCTGTGA TCAAAAAGAA GGCACCATCC GCCCCTTTGC CCATCCAGGA
    AGCCCCCGAA TGCCCCGTCC CTTGGGGATG TGGTCCTGTG CAGACACCGA CTCCACCCCA
    TTCGAAGACC GGCCGCTGTC CAAGCTGAAG GAATCAGACA GATGTTCAGC ACGGGAAAAC
    CTCTACTTGG ACGCCTTGTC TCTAGATGAC GAGCCAGAGG AGCCTCCAGC CCGAGAGCCC
    CAGCGGGAGT TCAGGAACTG CCTCCCCCAG GAAGAAGAAA ATCACAAGGG AAATCTTCAA
    AGGGCCGTGT CTGTGTCCTC CATGTCTGAG TTCCAGCGTC TGATGGACGT TTCTCCCTTC
    CTGCCGGAGA AGGGCGTCCC CGCCACCAGC AGCAAGGAGG ACATCACTCC GCCCCTGTCT
    CCTGATGATC TGAAGTACAT CGAGGAGTTC AACAGCAAGA ACTGGGACGA GAGGCCCCCG
    GAGCCCTGGG CAGACAGGAC CGAGGGGGGG CGGGCAGGGA GCGAGGCTGG TGCAGAGCCT
    TTCCCTGACT CCTCCTGGTA CCTCACCACG AGTGTCACCA TGACTACGGA CACCATGACC
    AGCCCAGAGC ACTGCCAGAA GCAGCCCCTT CGGAGCCCCA TGTGCGCGGA GCAGGCCGGG
    GTGCGGGTGC TGCACAGCCC GCCCGCGGTC CGTAGGATGG ATGGCATCGT GGTGGCGGGC
    AGTGAGGGCA AGGGCCGGGC CGAGCCCGAG TGCCCGTTTC CAACTAGCAG AGCCAGGGGG
    GGCACTGGAG ACACAAAAGG GGGTCCCTCA GAACACGTGC TCAGCAGGTG GCCTTGCGCC
    CCCTCCAGGC ACCCCCGAGA CTATGTGGAG GGAGGGCTGC GCCCCCTCGA AAGTGCCCTC
    TGCGCCCCCC TGGGTTTTGC CTCCTCGCTG CACGGCCTGC AGATGTCCAA GAACATGAGT
    GATGACATGA AGGAGGTGGC CTTCTCGGTC AGGAACGCCA TCTGCTCTGG CCCCCCCAAG
    CCTCCGCTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGACGGG AACACAGACC
    GTCCAGACCA TCACCATTGG CCTGCAGACC GAAGCCCTGC GTGGCAGCGC CGTCACCAGC
    AGCCCCCACA AGTGTCTCAC ACCCAAGGCC GGGGGCGGGG CCACGCCCGC GTCATCTCCT
    TCCCGTGGCC TTCGGAGCCG GCAGATGGCC CCCGCCATCG AGAAGGTGCA GGCCAAGTTC
    GAACGCACGT GCTGCTCCCC CAAGTACGGG TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC
    AAAGCTGAGC CGCCCAACAG CAGGACCTCC CTGGGGCTGG CCCCAAAGGG GTGCAGCGAG
    TCCGCCTGGG CCCGTTCCAC CACCACGAGG GAGAGCCCGG TGCACACCGC CATCAACGAT
    GGCCTCTCCA GCCTCTTCAA CATCATCGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG
    AAGGGGACTC GGGCCGGGAG CCGATCTCGC TCAGCAGAAC CCCGGCCCGA GCTGGGCCCA
    GGCCAGGAAA CGGGCCCCAG TTCCCGGGGC CGGTCCCCCA GCCCCATCGG GGCTGGCTCA
    GAGACTTGGA GGGAGGAAGG GGGAGAGGGC ACGCCCTTGA GGCAGGACTT ATCCGCTCCC
    CCTGGTTACA CGTTCACTGA GAACGTAGCC CGGATCCTCA ACAAGAAGCT GTTGGAGCAT
    GCCTTAAAGG AGGAGAGGAG GCAGGCCCCC CACGGGCCCC CAAGTCTTAA CAGTGACAGC
    CACATGGGAG AAACCATCAA AGCTGAACCA GGGTCCATCG AGGAACTACC TTGTTCCGCA
    CTAGCTCCAT CCCTAGAACC CTGCTTCTCC AGGCCCGAGA GACCAGCAAA CCGTCGCCTT
    CCGTCCCGTT GGGCCTCACA TTCCCCCACT GCCTCACTGG CTCAGTCACC CGGAGACCCA
    GCCTCCTTGG AGGAGCTCGG CGATGAGGAG CCACCGGGGG AGAAGACACA CCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022277328.1
    CDS117..5153
    Translation

    Target ORF information:

    RefSeq Version XM_022421620.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022421620.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    CAGAGTGGTG GAACCGAGCG CCTTGGAAAC TGCAGGTCTA CAACCAAAAC TCAAAGGAAA
    CTGGCACCCC GGCGCAAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA
    GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG
    GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG
    GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG
    GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA
    GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG
    GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG
    CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT
    GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT
    CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT
    CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG
    AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC
    CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG
    AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC
    GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA
    TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC
    CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG
    GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG
    CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG
    GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG
    GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC
    CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC
    GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGTCCA AACTGAGAGG TCAGCTGGAG
    TGGCAGCCGG GGCAGGAGTG CGGGGACGAG CCAGAGCAGC TGCACCGCCG CGCTGATGGG
    GACGTCGGGA GCTGCCGGCA GGGAGGCCAG AGCTGCTTCA GTCTAGAGAG TGTATCGCTA
    GAGGAGCTGC AGGGTCAGCT TGCGCAGGCG ACCAGACTGC ATCAAGAGGA GACAGAGAAA
    TTTACAAACA AGATTCGGAA GATGGAGGAG GAGCACCTGT ACGCCTTGAG GTGGAAAGAA
    CTGGAAATGC ACAGCCTGGC TTTGCAGAAC ACCCTCCATG AGCAAACCTG GAGTGATGAG
    AGGAACCTGA TGCAGCAGGA GCTCCGGTCT ATGAAGCAGA ACATTTTCCT TTTCTACGTC
    AAACTCAGAT GGCTGCTGAG GCACTGGCGA CAAGGGAAGC AGATGGAGGA AGAAGGCGAG
    GATTTCACTG AGAGCGAGCA TCCAGAGACC CTCCCCAGGC TTGGTGAGCT TGGAGTGCAG
    GGGGACCCGA AGGGGGACGG CCCAGACCAA GATGACAACG GTCCGGGCTG CGGCTTTCCG
    CTGGGAGAGC ATCCTCCACA CTCCCCTGTG CAGATTGGTG ACCATGGATC GAGACTACAG
    CCTACACATG GGAGACAGCT CCACAGGCAG GTGGTGGAAA ACCAGCAGCT GTTCAGTGCA
    CTCAAGAGCC TGCTGGAGGA CCTGCGCGCC GAGCTGCGGG AGGACGAGCA TGCACGGCGG
    CGGCTGCAGC AGCAGTACGC CAGCGACAAA GCCGCCTGGG ACGTGGAGTG GGCTGCACTT
    ACCTGCCGCC TGGAACAGCT GGAAGAGAAG ACTGAGAAGA ATTTGGGAGA AGCATGCTCT
    TCCACCGACA GCAAAGGGGC ATTCAAGAAG GAAAGAGAGA TGCATCAGAA GCTCCTCACT
    GACAGTCATG GCCTGGTCAT GGACCTGCGC TGGCAGATCC ATCACAGTGA GAAGAACTGG
    AGCCGGGAGA AGGTGGAACT TCTTAGCCGT CTAGACAGAG ACCGGCAGGA ATGGGAGCAG
    CAGAAGAAGG AGCTCTTGTG GAGAATAGAA CAGTTGCAGA AAGAAAACAG TCCCCGGAGA
    AGTGGCAGTT TCCTCTGTGA TCAAAAAGAA GGCACCATCC GCCCCTTTGC CCATCCAGGA
    AGCCCCCGAA TGCCCCGTCC CTTGGGGATG TGGTCCTGTG CAGACACCGA CTCCACCCCA
    TTCGAAGACC GGCCGCTGTC CAAGCTGAAG GAATCAGACA GATGTTCAGC ACGGGAAAAC
    CTCTACTTGG ACGCCTTGTC TCTAGATGAC GAGCCAGAGG AGCCTCCAGC CCGAGAGCCC
    CAGCGGGAGT TCAGGAACTG CCTCCCCCAG GAAGAAGAAA ATCACAAGGG AAATCTTCAA
    AGGGCCGTGT CTGTGTCCTC CATGTCTGAG TTCCAGCGTC TGATGGACGT TTCTCCCTTC
    CTGCCGGAGA AGGGCGTCCC CGCCACCAGC AGCAAGGAGG ACATCACTCC GCCCCTGTCT
    CCTGATGATC TGAAGTACAT CGAGGAGTTC AACAGCAAGA ACTGGGACGA GAGGCCCCCG
    GAGCCCTGGG CAGACAGGAC CGAGGGGGGG CGGGCAGGGA GCGAGGCTGG TGCAGAGCCT
    TTCCCTGACT CCTCCTGGTA CCTCACCACG AGTGTCACCA TGACTACGGA CACCATGACC
    AGCCCAGAGC ACTGCCAGAA GCAGCCCCTT CGGAGCCCCA TGTGCGCGGA GCAGGCCGGG
    GTGCGGGTGC TGCACAGCCC GCCCGCGGTC CGTAGGATGG ATGGCATCGT GGTGGCGGGC
    AGTGAGGGCA AGGGCCGGGC CGAGCCCGAG TGCCCGTTTC CAACTAGCAG AGCCAGGGGG
    GGCACTGGAG ACACAAAAGG GGGTCCCTCA GAACACGTGC TCAGCAGGTG GCCTTGCGCC
    CCCTCCAGGC ACCCCCGAGA CTATGTGGAG GGAGGGCTGC GCCCCCTCGA AAGTGCCCTC
    TGCGCCCCCC TGGGTTTTGC CTCCTCGCTG CACGGCCTGC AGATGTCCAA GAACATGAGT
    GATGACATGA AGGAGGTGGC CTTCTCGGTC AGGAACGCCA TCTGCTCTGG CCCCCCCAAG
    CCTCCGCTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGACGGG AACACAGACC
    GTCCAGACCA TCACCATTGG CCTGCAGACC GAAGCCCTGC GTGGCAGCGC CGTCACCAGC
    AGCCCCCACA AGTGTCTCAC ACCCAAGGCC GGGGGCGGGG CCACGCCCGC GTCATCTCCT
    TCCCGTGGCC TTCGGAGCCG GCAGATGGCC CCCGCCATCG AGAAGGTGCA GGCCAAGTTC
    GAACGCACGT GCTGCTCCCC CAAGTACGGG TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC
    AAAGCTGAGC CGCCCAACAG CAGGACCTCC CTGGGGCTGG CCCCAAAGGG GTGCAGCGAG
    TCCGCCTGGG CCCGTTCCAC CACCACGAGG GAGAGCCCGG TGCACACCGC CATCAACGAT
    GGCCTCTCCA GCCTCTTCAA CATCATCGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG
    AAGGGGACTC GGGCCGGGAG CCGATCTCGC TCAGCAGAAC CCCGGCCCGA GCTGGGCCCA
    GGCCAGGAAA CGGGCCCCAG TTCCCGGGGC CGGTCCCCCA GCCCCATCGG GGCTGGCTCA
    GAGACTTGGA GGGAGGAAGG GGGAGAGGGC ACGCCCTTGA GGCAGGACTT ATCCGCTCCC
    CCTGGTTACA CGTTCACTGA GAACGTAGCC CGGATCCTCA ACAAGAAGCT GTTGGAGCAT
    GCCTTAAAGG AGGAGAGGAG GCAGGCCCCC CACGGGCCCC CAAGTCTTAA CAGTGACAGC
    CACATGGGAG AAACCATCAA AGCTGAACCA GGGTCCATCG AGGAACTACC TTGTTCCGCA
    CTAGCTCCAT CCCTAGAACC CTGCTTCTCC AGGCCCGAGA GACCAGCAAA CCGTCGCCTT
    CCGTCCCGTT GGGCCTCACA TTCCCCCACT GCCTCACTGG CTCAGTCACC CGGAGACCCA
    GCCTCCTTGG AGGAGCTCGG CGATGAGGAG CCACCGGGGG AGAAGACACA CCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe289879
    Clone ID Related Accession (Same CDS sequence) XM_022421619.1
    Accession Version XM_022421619.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5094bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product microtubule cross-linking factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    AGTGGTGGAA CCGAGCGCCT TGGAAACTGC AGGTCTACAA CCAAAACTCA AAGGAAACTG
    GCACCCCGGC GCAAGGATGA CAGTGCCGAT TTGAAGTGCC AGCTTCAGTT TGCCAAAGAG
    GAAGGCTCCC TGATGCGTAA AAAGATGGCC AAGCTGGGGA AGGAGAAGGA CGAGCTGGAA
    CAGGAGCTCC AGAAGTACAA GGCCCTCTAT GGAGACGTGG ACAGCCCCCT CCCCACGGGG
    GAAGCCGGGG GGCCGCCCAG CACCAGGGAG GCTGAGCTGA AGCTGCGCTT GAAGTTGGTG
    GAGGAAGAGG CCAACATCTT GGGCCGGAAG ATTGTGGAAC TGGAGGTGGA GAACCGAGGC
    CTCAAAGCAG AAATGGAGGA CATGAGGCTC CAGTACGAGC GAGAGGGTAC AGGCCGGGAC
    CACATCACAG GCATTCCTAC CTCACCCTTC GGTGACTCCC TGGAGTCCTC GACGGAGCTC
    CGCCGGCACC TGCAGTTCGT GGAGGAGGAA GCGGAGCTGC TCCGGAGGTC CATATCTGAG
    ATCGAAGACC ACAACCGGCA GCTGACCCAT GAGCTGAACA AGTTCAAGTT TGAGCCTCGG
    CCGGAGCCAG GGTGGCTGGC GGATGGCGCG GGCAAGGGCG TGGGCAGCGG GGCCCCTCTG
    CAGGAGGAGC TCAAGTCGGC ACGGCTACAG ATCAATGAGC TCAGTGGGAA GGTGCTGAAG
    CTGCAGTATG AGAACCGGGT GCTACTGTCC AATGTGCAGC GCTATGACCT GGCTGCCCAC
    CTAGGCCTGC GTGCCCCCAG CCCCCGGGAC AGCGACGCAG ACAGCGACCA GGGCAAGAAG
    GAGAGCGATG GGGAGGAAGG CCGCCAGCCC CAGCCCAAGC GGGAGGGCCC TATTGGCGGG
    GAGAGCGACT CAGAGGAGAT GTGCGAGAAG ACGTCGGGCT TCGGCAGCGG AAAGCCATCG
    GAGGCGAGCG AGCCATGTCC CGCCGAGCTC CTGCGGGCCA GGGAGGACAC CGAGTGCCTG
    GCGAGCATAA AGCACGAGGC TGAGCGCCTG GAGCGAACAG TGGAGCGCCT CATCACGGAC
    ACCGCGGGCT TCGGGGACGA TGTGGGGCTG CTGGGCAGTG GCGAGCCCTT GCCAGGGCCC
    GACATCCAGG GGGCGGGGGG GACGGGCGAG GGGGGCAAGA AGGAGCCCCC ACTGCTGGGG
    ACCATCAACT CGAGGATGAA GGCTTTCAGG AAGGAGCTGC AGGCCTTCCT GGAGCAGGTG
    AACCGAATCG GGGATGGCCT GCGGGAGCGC CTGGAAGGCC TGTCCCCCCT GCCTCACCTC
    ACAGAGTCTT CCAGCTTTCT CTCCACCGTC ACTTCCGTGT CCCGGGACTC CCCCGTCGGG
    AACCTGGGGA AGGAGCTGGT CCCGGACCTG CAGTCCAAAC TGAGAGGTCA GCTGGAGTGG
    CAGCCGGGGC AGGAGTGCGG GGACGAGCCA GAGCAGCTGC ACCGCCGCGC TGATGGGGAC
    GTCGGGAGCT GCCGGCAGGG AGGCCAGAGC TGCTTCAGTC TAGAGAGTGT ATCGCTAGAG
    GAGCTGCAGG GTCAGCTTGC GCAGGCGACC AGACTGCATC AAGAGGAGAC AGAGAAATTT
    ACAAACAAGA TTCGGAAGAT GGAGGAGGAG CACCTGTACG CCTTGAGGTG GAAAGAACTG
    GAAATGCACA GCCTGGCTTT GCAGAACACC CTCCATGAGC AAACCTGGAG TGATGAGAGG
    AACCTGATGC AGCAGGAGCT CCGGTCTATG AAGCAGAACA TTTTCCTTTT CTACGTCAAA
    CTCAGATGGC TGCTGAGGCA CTGGCGACAA GGGAAGCAGA TGGAGGAAGA AGGCGAGGAT
    TTCACTGAGA GCGAGCATCC AGAGACCCTC CCCAGGCTTG GTGAGCTTGG AGTGCAGGGG
    GACCCGAAGG GGGACGGCCC AGACCAAGAT GACAACGGTC CGGGCTGCGG CTTTCCGCTG
    GGAGAGCATC CTCCACACTC CCCTGTGCAG ATTGGTGACC ATGGATCGAG ACTACAGCCT
    ACACATGGGA GACAGCTCCA CAGGCAGGTG GTGGAAAACC AGCAGCTGTT CAGTGCACTC
    AAGAGCCTGC TGGAGGACCT GCGCGCCGAG CTGCGGGAGG ACGAGCATGC ACGGCGGCGG
    CTGCAGCAGC AGTACGCCAG CGACAAAGCC GCCTGGGACG TGGAGTGGGC TGCACTTACC
    TGCCGCCTGG AACAGGTGCC TGCCTGCGGG GTCTGTCCTG GGTCTCCCCA GCTCCTCCTC
    TGCCATCTGG GCCAGCTGGA AGAGAAGACT GAGAAGAATT TGGGAGAAGC ATGCTCTTCC
    ACCGACAGCA AAGGGGCATT CAAGAAGGAA AGAGAGATGC ATCAGAAGCT CCTCACTGAC
    AGTCATGGCC TGGTCATGGA CCTGCGCTGG CAGATCCATC ACAGTGAGAA GAACTGGAGC
    CGGGAGAAGG TGGAACTTCT TAGCCGTCTA GACAGAGACC GGCAGGAATG GGAGCAGCAG
    AAGAAGGAGC TCTTGTGGAG AATAGAACAG TTGCAGAAAG AAAACAGTCC CCGGAGAAGT
    GGCAGTTTCC TCTGTGATCA AAAAGAAGGC ACCATCCGCC CCTTTGCCCA TCCAGGAAGC
    CCCCGAATGC CCCGTCCCTT GGGGATGTGG TCCTGTGCAG ACACCGACTC CACCCCATTC
    GAAGACCGGC CGCTGTCCAA GCTGAAGGAA TCAGACAGAT GTTCAGCACG GGAAAACCTC
    TACTTGGACG CCTTGTCTCT AGATGACGAG CCAGAGGAGC CTCCAGCCCG AGAGCCCCAG
    CGGGAGTTCA GGAACTGCCT CCCCCAGGAA GAAGAAAATC ACAAGGGAAA TCTTCAAAGG
    GCCGTGTCTG TGTCCTCCAT GTCTGAGTTC CAGCGTCTGA TGGACGTTTC TCCCTTCCTG
    CCGGAGAAGG GCGTCCCCGC CACCAGCAGC AAGGAGGACA TCACTCCGCC CCTGTCTCCT
    GATGATCTGA AGTACATCGA GGAGTTCAAC AGCAAGAACT GGGACGAGAG GCCCCCGGAG
    CCCTGGGCAG ACAGGACCGA GGGGGGGCGG GCAGGGAGCG AGGCTGGTGC AGAGCCTTTC
    CCTGACTCCT CCTGGTACCT CACCACGAGT GTCACCATGA CTACGGACAC CATGACCAGC
    CCAGAGCACT GCCAGAAGCA GCCCCTTCGG AGCCCCATGT GCGCGGAGCA GGCCGGGGTG
    CGGGTGCTGC ACAGCCCGCC CGCGGTCCGT AGGATGGATG GCATCGTGGT GGCGGGCAGT
    GAGGGCAAGG GCCGGGCCGA GCCCGAGTGC CCGTTTCCAA CTAGCAGAGC CAGGGGGGGC
    ACTGGAGACA CAAAAGGGGG TCCCTCAGAA CACGTGCTCA GCAGGTGGCC TTGCGCCCCC
    TCCAGGCACC CCCGAGACTA TGTGGAGGGA GGGCTGCGCC CCCTCGAAAG TGCCCTCTGC
    GCCCCCCTGG GTTTTGCCTC CTCGCTGCAC GGCCTGCAGA TGTCCAAGAA CATGAGTGAT
    GACATGAAGG AGGTGGCCTT CTCGGTCAGG AACGCCATCT GCTCTGGCCC CCCCAAGCCT
    CCGCTCAAGG ACATGGCCTG CCAGACCAAT GGGTCCCGGA CGACGGGAAC ACAGACCGTC
    CAGACCATCA CCATTGGCCT GCAGACCGAA GCCCTGCGTG GCAGCGCCGT CACCAGCAGC
    CCCCACAAGT GTCTCACACC CAAGGCCGGG GGCGGGGCCA CGCCCGCGTC ATCTCCTTCC
    CGTGGCCTTC GGAGCCGGCA GATGGCCCCC GCCATCGAGA AGGTGCAGGC CAAGTTCGAA
    CGCACGTGCT GCTCCCCCAA GTACGGGTCT CCCAAGCTGC AGAGGAAGCC CCTCCCCAAA
    GCTGAGCCGC CCAACAGCAG GACCTCCCTG GGGCTGGCCC CAAAGGGGTG CAGCGAGTCC
    GCCTGGGCCC GTTCCACCAC CACGAGGGAG AGCCCGGTGC ACACCGCCAT CAACGATGGC
    CTCTCCAGCC TCTTCAACAT CATCGACCAC AGCCCCGTGG TGCAGGACCC CTTCCAGAAG
    GGGACTCGGG CCGGGAGCCG ATCTCGCTCA GCAGAACCCC GGCCCGAGCT GGGCCCAGGC
    CAGGAAACGG GCCCCAGTTC CCGGGGCCGG TCCCCCAGCC CCATCGGGGC TGGCTCAGAG
    ACTTGGAGGG AGGAAGGGGG AGAGGGCACG CCCTTGAGGC AGGACTTATC CGCTCCCCCT
    GGTTACACGT TCACTGAGAA CGTAGCCCGG ATCCTCAACA AGAAGCTGTT GGAGCATGCC
    TTAAAGGAGG AGAGGAGGCA GGCCCCCCAC GGGCCCCCAA GTCTTAACAG TGACAGCCAC
    ATGGGAGAAA CCATCAAAGC TGAACCAGGG TCCATCGAGG AACTACCTTG TTCCGCACTA
    GCTCCATCCC TAGAACCCTG CTTCTCCAGG CCCGAGAGAC CAGCAAACCG TCGCCTTCCG
    TCCCGTTGGG CCTCACATTC CCCCACTGCC TCACTGGCTC AGTCACCCGG AGACCCAGCC
    TCCTTGGAGG AGCTCGGCGA TGAGGAGCCA CCGGGGGAGA AGACACACCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022277327.1
    CDS117..5210
    Translation

    Target ORF information:

    RefSeq Version XM_022421619.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022421619.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    AGTGGTGGAA CCGAGCGCCT TGGAAACTGC AGGTCTACAA CCAAAACTCA AAGGAAACTG
    GCACCCCGGC GCAAGGATGA CAGTGCCGAT TTGAAGTGCC AGCTTCAGTT TGCCAAAGAG
    GAAGGCTCCC TGATGCGTAA AAAGATGGCC AAGCTGGGGA AGGAGAAGGA CGAGCTGGAA
    CAGGAGCTCC AGAAGTACAA GGCCCTCTAT GGAGACGTGG ACAGCCCCCT CCCCACGGGG
    GAAGCCGGGG GGCCGCCCAG CACCAGGGAG GCTGAGCTGA AGCTGCGCTT GAAGTTGGTG
    GAGGAAGAGG CCAACATCTT GGGCCGGAAG ATTGTGGAAC TGGAGGTGGA GAACCGAGGC
    CTCAAAGCAG AAATGGAGGA CATGAGGCTC CAGTACGAGC GAGAGGGTAC AGGCCGGGAC
    CACATCACAG GCATTCCTAC CTCACCCTTC GGTGACTCCC TGGAGTCCTC GACGGAGCTC
    CGCCGGCACC TGCAGTTCGT GGAGGAGGAA GCGGAGCTGC TCCGGAGGTC CATATCTGAG
    ATCGAAGACC ACAACCGGCA GCTGACCCAT GAGCTGAACA AGTTCAAGTT TGAGCCTCGG
    CCGGAGCCAG GGTGGCTGGC GGATGGCGCG GGCAAGGGCG TGGGCAGCGG GGCCCCTCTG
    CAGGAGGAGC TCAAGTCGGC ACGGCTACAG ATCAATGAGC TCAGTGGGAA GGTGCTGAAG
    CTGCAGTATG AGAACCGGGT GCTACTGTCC AATGTGCAGC GCTATGACCT GGCTGCCCAC
    CTAGGCCTGC GTGCCCCCAG CCCCCGGGAC AGCGACGCAG ACAGCGACCA GGGCAAGAAG
    GAGAGCGATG GGGAGGAAGG CCGCCAGCCC CAGCCCAAGC GGGAGGGCCC TATTGGCGGG
    GAGAGCGACT CAGAGGAGAT GTGCGAGAAG ACGTCGGGCT TCGGCAGCGG AAAGCCATCG
    GAGGCGAGCG AGCCATGTCC CGCCGAGCTC CTGCGGGCCA GGGAGGACAC CGAGTGCCTG
    GCGAGCATAA AGCACGAGGC TGAGCGCCTG GAGCGAACAG TGGAGCGCCT CATCACGGAC
    ACCGCGGGCT TCGGGGACGA TGTGGGGCTG CTGGGCAGTG GCGAGCCCTT GCCAGGGCCC
    GACATCCAGG GGGCGGGGGG GACGGGCGAG GGGGGCAAGA AGGAGCCCCC ACTGCTGGGG
    ACCATCAACT CGAGGATGAA GGCTTTCAGG AAGGAGCTGC AGGCCTTCCT GGAGCAGGTG
    AACCGAATCG GGGATGGCCT GCGGGAGCGC CTGGAAGGCC TGTCCCCCCT GCCTCACCTC
    ACAGAGTCTT CCAGCTTTCT CTCCACCGTC ACTTCCGTGT CCCGGGACTC CCCCGTCGGG
    AACCTGGGGA AGGAGCTGGT CCCGGACCTG CAGTCCAAAC TGAGAGGTCA GCTGGAGTGG
    CAGCCGGGGC AGGAGTGCGG GGACGAGCCA GAGCAGCTGC ACCGCCGCGC TGATGGGGAC
    GTCGGGAGCT GCCGGCAGGG AGGCCAGAGC TGCTTCAGTC TAGAGAGTGT ATCGCTAGAG
    GAGCTGCAGG GTCAGCTTGC GCAGGCGACC AGACTGCATC AAGAGGAGAC AGAGAAATTT
    ACAAACAAGA TTCGGAAGAT GGAGGAGGAG CACCTGTACG CCTTGAGGTG GAAAGAACTG
    GAAATGCACA GCCTGGCTTT GCAGAACACC CTCCATGAGC AAACCTGGAG TGATGAGAGG
    AACCTGATGC AGCAGGAGCT CCGGTCTATG AAGCAGAACA TTTTCCTTTT CTACGTCAAA
    CTCAGATGGC TGCTGAGGCA CTGGCGACAA GGGAAGCAGA TGGAGGAAGA AGGCGAGGAT
    TTCACTGAGA GCGAGCATCC AGAGACCCTC CCCAGGCTTG GTGAGCTTGG AGTGCAGGGG
    GACCCGAAGG GGGACGGCCC AGACCAAGAT GACAACGGTC CGGGCTGCGG CTTTCCGCTG
    GGAGAGCATC CTCCACACTC CCCTGTGCAG ATTGGTGACC ATGGATCGAG ACTACAGCCT
    ACACATGGGA GACAGCTCCA CAGGCAGGTG GTGGAAAACC AGCAGCTGTT CAGTGCACTC
    AAGAGCCTGC TGGAGGACCT GCGCGCCGAG CTGCGGGAGG ACGAGCATGC ACGGCGGCGG
    CTGCAGCAGC AGTACGCCAG CGACAAAGCC GCCTGGGACG TGGAGTGGGC TGCACTTACC
    TGCCGCCTGG AACAGGTGCC TGCCTGCGGG GTCTGTCCTG GGTCTCCCCA GCTCCTCCTC
    TGCCATCTGG GCCAGCTGGA AGAGAAGACT GAGAAGAATT TGGGAGAAGC ATGCTCTTCC
    ACCGACAGCA AAGGGGCATT CAAGAAGGAA AGAGAGATGC ATCAGAAGCT CCTCACTGAC
    AGTCATGGCC TGGTCATGGA CCTGCGCTGG CAGATCCATC ACAGTGAGAA GAACTGGAGC
    CGGGAGAAGG TGGAACTTCT TAGCCGTCTA GACAGAGACC GGCAGGAATG GGAGCAGCAG
    AAGAAGGAGC TCTTGTGGAG AATAGAACAG TTGCAGAAAG AAAACAGTCC CCGGAGAAGT
    GGCAGTTTCC TCTGTGATCA AAAAGAAGGC ACCATCCGCC CCTTTGCCCA TCCAGGAAGC
    CCCCGAATGC CCCGTCCCTT GGGGATGTGG TCCTGTGCAG ACACCGACTC CACCCCATTC
    GAAGACCGGC CGCTGTCCAA GCTGAAGGAA TCAGACAGAT GTTCAGCACG GGAAAACCTC
    TACTTGGACG CCTTGTCTCT AGATGACGAG CCAGAGGAGC CTCCAGCCCG AGAGCCCCAG
    CGGGAGTTCA GGAACTGCCT CCCCCAGGAA GAAGAAAATC ACAAGGGAAA TCTTCAAAGG
    GCCGTGTCTG TGTCCTCCAT GTCTGAGTTC CAGCGTCTGA TGGACGTTTC TCCCTTCCTG
    CCGGAGAAGG GCGTCCCCGC CACCAGCAGC AAGGAGGACA TCACTCCGCC CCTGTCTCCT
    GATGATCTGA AGTACATCGA GGAGTTCAAC AGCAAGAACT GGGACGAGAG GCCCCCGGAG
    CCCTGGGCAG ACAGGACCGA GGGGGGGCGG GCAGGGAGCG AGGCTGGTGC AGAGCCTTTC
    CCTGACTCCT CCTGGTACCT CACCACGAGT GTCACCATGA CTACGGACAC CATGACCAGC
    CCAGAGCACT GCCAGAAGCA GCCCCTTCGG AGCCCCATGT GCGCGGAGCA GGCCGGGGTG
    CGGGTGCTGC ACAGCCCGCC CGCGGTCCGT AGGATGGATG GCATCGTGGT GGCGGGCAGT
    GAGGGCAAGG GCCGGGCCGA GCCCGAGTGC CCGTTTCCAA CTAGCAGAGC CAGGGGGGGC
    ACTGGAGACA CAAAAGGGGG TCCCTCAGAA CACGTGCTCA GCAGGTGGCC TTGCGCCCCC
    TCCAGGCACC CCCGAGACTA TGTGGAGGGA GGGCTGCGCC CCCTCGAAAG TGCCCTCTGC
    GCCCCCCTGG GTTTTGCCTC CTCGCTGCAC GGCCTGCAGA TGTCCAAGAA CATGAGTGAT
    GACATGAAGG AGGTGGCCTT CTCGGTCAGG AACGCCATCT GCTCTGGCCC CCCCAAGCCT
    CCGCTCAAGG ACATGGCCTG CCAGACCAAT GGGTCCCGGA CGACGGGAAC ACAGACCGTC
    CAGACCATCA CCATTGGCCT GCAGACCGAA GCCCTGCGTG GCAGCGCCGT CACCAGCAGC
    CCCCACAAGT GTCTCACACC CAAGGCCGGG GGCGGGGCCA CGCCCGCGTC ATCTCCTTCC
    CGTGGCCTTC GGAGCCGGCA GATGGCCCCC GCCATCGAGA AGGTGCAGGC CAAGTTCGAA
    CGCACGTGCT GCTCCCCCAA GTACGGGTCT CCCAAGCTGC AGAGGAAGCC CCTCCCCAAA
    GCTGAGCCGC CCAACAGCAG GACCTCCCTG GGGCTGGCCC CAAAGGGGTG CAGCGAGTCC
    GCCTGGGCCC GTTCCACCAC CACGAGGGAG AGCCCGGTGC ACACCGCCAT CAACGATGGC
    CTCTCCAGCC TCTTCAACAT CATCGACCAC AGCCCCGTGG TGCAGGACCC CTTCCAGAAG
    GGGACTCGGG CCGGGAGCCG ATCTCGCTCA GCAGAACCCC GGCCCGAGCT GGGCCCAGGC
    CAGGAAACGG GCCCCAGTTC CCGGGGCCGG TCCCCCAGCC CCATCGGGGC TGGCTCAGAG
    ACTTGGAGGG AGGAAGGGGG AGAGGGCACG CCCTTGAGGC AGGACTTATC CGCTCCCCCT
    GGTTACACGT TCACTGAGAA CGTAGCCCGG ATCCTCAACA AGAAGCTGTT GGAGCATGCC
    TTAAAGGAGG AGAGGAGGCA GGCCCCCCAC GGGCCCCCAA GTCTTAACAG TGACAGCCAC
    ATGGGAGAAA CCATCAAAGC TGAACCAGGG TCCATCGAGG AACTACCTTG TTCCGCACTA
    GCTCCATCCC TAGAACCCTG CTTCTCCAGG CCCGAGAGAC CAGCAAACCG TCGCCTTCCG
    TCCCGTTGGG CCTCACATTC CCCCACTGCC TCACTGGCTC AGTCACCCGG AGACCCAGCC
    TCCTTGGAGG AGCTCGGCGA TGAGGAGCCA CCGGGGGAGA AGACACACCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe289880
    Clone ID Related Accession (Same CDS sequence) XM_022421621.1
    Accession Version XM_022421621.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5034bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product microtubule cross-linking factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAAGAGA TGAGAGACAG TTACTTAGAG GAAGATGTTT ACCAGCTGCA GGAGCTTCGG 
    CGAGAGCTGG ACCGTGCTCA TAAAAACTGC CGAATCCTGC AGTACCGCCT CAGGAAAGCT
    GAGCAGAAGA GCCTGAAAGT GGCTGAGACT GGTCAGGTGG ATGGTGAGCT CATCCGGAGT
    CTAGAGCAGG ATTTAAAGGT AGCCAAAGAT GTATCTGTCA GATTGCATCA TGAACTTGAA
    ACCGTGGAGG AAAAGCGTGC TAAAGCAGAG GATGAAAATG AAAGTCTCCG GCAGCAGATG
    ATTGAAGTGG AGATATCTAA ACAGGCCCTC CAAAATGAGC TGGAGAGACT GAAAGAGAGT
    TCCCTGAAAA GAAGAGGCAA CCGAGAAACA TACAAAGAAA AGAAAACCTT TAACCAGCAG
    AGTGGTGGAA CCGAGCGCCT TGGAAACTGC AGGTCTACAA CCAAAACTCA AAGGAAACTG
    GCACCCCGGC GCAAGGATGA CAGTGCCGAT TTGAAGTGCC AGCTTCAGTT TGCCAAAGAG
    GAAGGCTCCC TGATGCGTAA AAAGATGGCC AAGCTGGGGA AGGAGAAGGA CGAGCTGGAA
    CAGGAGCTCC AGAAGTACAA GGCCCTCTAT GGAGACGTGG ACAGCCCCCT CCCCACGGGG
    GAAGCCGGGG GGCCGCCCAG CACCAGGGAG GCTGAGCTGA AGCTGCGCTT GAAGTTGGTG
    GAGGAAGAGG CCAACATCTT GGGCCGGAAG ATTGTGGAAC TGGAGGTGGA GAACCGAGGC
    CTCAAAGCAG AAATGGAGGA CATGAGGCTC CAGTACGAGC GAGAGGGTAC AGGCCGGGAC
    CACATCACAG GCATTCCTAC CTCACCCTTC GGTGACTCCC TGGAGTCCTC GACGGAGCTC
    CGCCGGCACC TGCAGTTCGT GGAGGAGGAA GCGGAGCTGC TCCGGAGGTC CATATCTGAG
    ATCGAAGACC ACAACCGGCA GCTGACCCAT GAGCTGAACA AGTTCAAGTT TGAGCCTCGG
    CCGGAGCCAG GGTGGCTGGC GGATGGCGCG GGCAAGGGCG TGGGCAGCGG GGCCCCTCTG
    CAGGAGGAGC TCAAGTCGGC ACGGCTACAG ATCAATGAGC TCAGTGGGAA GGTGCTGAAG
    CTGCAGTATG AGAACCGGGT GCTACTGTCC AATGTGCAGC GCTATGACCT GGCTGCCCAC
    CTAGGCCTGC GTGCCCCCAG CCCCCGGGAC AGCGACGCAG ACAGCGACCA GGGCAAGAAG
    GAGAGCGATG GGGAGGAAGG CCGCCAGCCC CAGCCCAAGC GGGAGGGCCC TATTGGCGGG
    GAGAGCGACT CAGAGGAGAT GTGCGAGAAG ACGTCGGGCT TCGGCAGCGG AAAGCCATCG
    GAGGCGAGCG AGCCATGTCC CGCCGAGCTC CTGCGGGCCA GGGAGGACAC CGAGTGCCTG
    GCGAGCATAA AGCACGAGGC TGAGCGCCTG GAGCGAACAG TGGAGCGCCT CATCACGGAC
    ACCGCGGGCT TCGGGGACGA TGTGGGGCTG CTGGGCAGTG GCGAGCCCTT GCCAGGGCCC
    GACATCCAGG GGGCGGGGGG GACGGGCGAG GGGGGCAAGA AGGAGCCCCC ACTGCTGGGG
    ACCATCAACT CGAGGATGAA GGCTTTCAGG AAGGAGCTGC AGGCCTTCCT GGAGCAGGTG
    AACCGAATCG GGGATGGCCT GCGGGAGCGC CTGGAAGGCC TGTCCCCCCT GCCTCACCTC
    ACAGAGTCTT CCAGCTTTCT CTCCACCGTC ACTTCCGTGT CCCGGGACTC CCCCGTCGGG
    AACCTGGGGA AGGAGCTGGT CCCGGACCTG CAGTCCAAAC TGAGAGGTCA GCTGGAGTGG
    CAGCCGGGGC AGGAGTGCGG GGACGAGCCA GAGCAGCTGC ACCGCCGCGC TGATGGGGAC
    GTCGGGAGCT GCCGGCAGGG AGGCCAGAGC TGCTTCAGTC TAGAGAGTGT ATCGCTAGAG
    GAGCTGCAGG GTCAGCTTGC GCAGGCGACC AGACTGCATC AAGAGGAGAC AGAGAAATTT
    ACAAACAAGA TTCGGAAGAT GGAGGAGGAG CACCTGTACG CCTTGAGGTG GAAAGAACTG
    GAAATGCACA GCCTGGCTTT GCAGAACACC CTCCATGAGC AAACCTGGAG TGATGAGAGG
    AACCTGATGC AGCAGGAGCT CCGGTCTATG AAGCAGAACA TTTTCCTTTT CTACGTCAAA
    CTCAGATGGC TGCTGAGGCA CTGGCGACAA GGGAAGCAGA TGGAGGAAGA AGGCGAGGAT
    TTCACTGAGA GCGAGCATCC AGAGACCCTC CCCAGGCTTG GTGAGCTTGG AGTGCAGGGG
    GACCCGAAGG GGGACGGCCC AGACCAAGAT GACAACGGTC CGGGCTGCGG CTTTCCGCTG
    GGAGAGCATC CTCCACACTC CCCTGTGCAG ATTGGTGACC ATGGATCGAG ACTACAGCCT
    ACACATGGGA GACAGCTCCA CAGGCAGGTG GTGGAAAACC AGCAGCTGTT CAGTGCACTC
    AAGAGCCTGC TGGAGGACCT GCGCGCCGAG CTGCGGGAGG ACGAGCATGC ACGGCGGCGG
    CTGCAGCAGC AGTACGCCAG CGACAAAGCC GCCTGGGACG TGGAGTGGGC TGCACTTACC
    TGCCGCCTGG AACAGGTGCC TGCCTGCGGG GTCTGTCCTG GGTCTCCCCA GCTCCTCCTC
    TGCCATCTGG GCCAGCTGGA AGAGAAGACT GAGAAGAATT TGGGAGAAGC ATGCTCTTCC
    ACCGACAGCA AAGGGGCATT CAAGAAGGAA AGAGAGATGC ATCAGAAGCT CCTCACTGAC
    AGTCATGGCC TGGTCATGGA CCTGCGCTGG CAGATCCATC ACAGTGAGAA GAACTGGAGC
    CGGGAGAAGG TGGAACTTCT TAGCCGTCTA GACAGAGACC GGCAGGAATG GGAGCAGCAG
    AAGAAGGAGC TCTTGTGGAG AATAGAACAG TTGCAGAAAG AAAACAGTCC CCGGAGAAGT
    GGCAGTTTCC TCTGTGATCA AAAAGAAGGC ACCATCCGCC CCTTTGCCCA TCCAGGAAGC
    CCCCGAATGC CCCGTCCCTT GGGGATGTGG TCCTGTGCAG ACACCGACTC CACCCCATTC
    GAAGACCGGC CGCTGTCCAA GCTGAAGGAA TCAGACAGAT GTTCAGCACG GGAAAACCTC
    TACTTGGACG CCTTGTCTCT AGATGACGAG CCAGAGGAGC CTCCAGCCCG AGAGCCCCAG
    CGGGAGTTCA GGAACTGCCT CCCCCAGGAA GAAGAAAATC ACAAGGGAAA TCTTCAAAGG
    GCCGTGTCTG TGTCCTCCAT GTCTGAGTTC CAGCGTCTGA TGGACGTTTC TCCCTTCCTG
    CCGGAGAAGG GCGTCCCCGC CACCAGCAGC AAGGAGGACA TCACTCCGCC CCTGTCTCCT
    GATGATCTGA AGTACATCGA GGAGTTCAAC AGCAAGAACT GGGACGAGAG GCCCCCGGAG
    CCCTGGGCAG ACAGGACCGA GGGGGGGCGG GCAGGGAGCG AGGCTGGTGC AGAGCCTTTC
    CCTGACTCCT CCTGGTACCT CACCACGAGT GTCACCATGA CTACGGACAC CATGACCAGC
    CCAGAGCACT GCCAGAAGCA GCCCCTTCGG AGCCCCATGT GCGCGGAGCA GGCCGGGGTG
    CGGGTGCTGC ACAGCCCGCC CGCGGTCCGT AGGATGGATG GCATCGTGGT GGCGGGCAGT
    GAGGGCAAGG GCCGGGCCGA GCCCGAGTGC CCGTTTCCAA CTAGCAGAGC CAGGGGGGGC
    ACTGGAGACA CAAAAGGGGG TCCCTCAGAA CACGTGCTCA GCAGGTGGCC TTGCGCCCCC
    TCCAGGCACC CCCGAGACTA TGTGGAGGGA GGGCTGCGCC CCCTCGAAAG TGCCCTCTGC
    GCCCCCCTGG GTTTTGCCTC CTCGCTGCAC GGCCTGCAGA TGTCCAAGAA CATGAGTGAT
    GACATGAAGG AGGTGGCCTT CTCGGTCAGG AACGCCATCT GCTCTGGCCC CCCCAAGCCT
    CCGCTCAAGG ACATGGCCTG CCAGACCAAT GGGTCCCGGA CGACGGGAAC ACAGACCGTC
    CAGACCATCA CCATTGGCCT GCAGACCGAA GCCCTGCGTG GCAGCGCCGT CACCAGCAGC
    CCCCACAAGT GTCTCACACC CAAGGCCGGG GGCGGGGCCA CGCCCGCGTC ATCTCCTTCC
    CGTGGCCTTC GGAGCCGGCA GATGGCCCCC GCCATCGAGA AGGTGCAGGC CAAGTTCGAA
    CGCACGTGCT GCTCCCCCAA GTACGGGTCT CCCAAGCTGC AGAGGAAGCC CCTCCCCAAA
    GCTGAGCCGC CCAACAGCAG GACCTCCCTG GGGCTGGCCC CAAAGGGGTG CAGCGAGTCC
    GCCTGGGCCC GTTCCACCAC CACGAGGGAG AGCCCGGTGC ACACCGCCAT CAACGATGGC
    CTCTCCAGCC TCTTCAACAT CATCGACCAC AGCCCCGTGG TGCAGGACCC CTTCCAGAAG
    GGGACTCGGG CCGGGAGCCG ATCTCGCTCA GCAGAACCCC GGCCCGAGCT GGGCCCAGGC
    CAGGAAACGG GCCCCAGTTC CCGGGGCCGG TCCCCCAGCC CCATCGGGGC TGGCTCAGAG
    ACTTGGAGGG AGGAAGGGGG AGAGGGCACG CCCTTGAGGC AGGACTTATC CGCTCCCCCT
    GGTTACACGT TCACTGAGAA CGTAGCCCGG ATCCTCAACA AGAAGCTGTT GGAGCATGCC
    TTAAAGGAGG AGAGGAGGCA GGCCCCCCAC GGGCCCCCAA GTCTTAACAG TGACAGCCAC
    ATGGGAGAAA CCATCAAAGC TGAACCAGGG TCCATCGAGG AACTACCTTG TTCCGCACTA
    GCTCCATCCC TAGAACCCTG CTTCTCCAGG CCCGAGAGAC CAGCAAACCG TCGCCTTCCG
    TCCCGTTGGG CCTCACATTC CCCCACTGCC TCACTGGCTC AGTCACCCGG AGACCCAGCC
    TCCTTGGAGG AGCTCGGCGA TGAGGAGCCA CCGGGGGAGA AGACACACCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022277329.1
    CDS1029..6062
    Translation

    Target ORF information:

    RefSeq Version XM_022421621.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022421621.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAAGAGA TGAGAGACAG TTACTTAGAG GAAGATGTTT ACCAGCTGCA GGAGCTTCGG 
    CGAGAGCTGG ACCGTGCTCA TAAAAACTGC CGAATCCTGC AGTACCGCCT CAGGAAAGCT
    GAGCAGAAGA GCCTGAAAGT GGCTGAGACT GGTCAGGTGG ATGGTGAGCT CATCCGGAGT
    CTAGAGCAGG ATTTAAAGGT AGCCAAAGAT GTATCTGTCA GATTGCATCA TGAACTTGAA
    ACCGTGGAGG AAAAGCGTGC TAAAGCAGAG GATGAAAATG AAAGTCTCCG GCAGCAGATG
    ATTGAAGTGG AGATATCTAA ACAGGCCCTC CAAAATGAGC TGGAGAGACT GAAAGAGAGT
    TCCCTGAAAA GAAGAGGCAA CCGAGAAACA TACAAAGAAA AGAAAACCTT TAACCAGCAG
    AGTGGTGGAA CCGAGCGCCT TGGAAACTGC AGGTCTACAA CCAAAACTCA AAGGAAACTG
    GCACCCCGGC GCAAGGATGA CAGTGCCGAT TTGAAGTGCC AGCTTCAGTT TGCCAAAGAG
    GAAGGCTCCC TGATGCGTAA AAAGATGGCC AAGCTGGGGA AGGAGAAGGA CGAGCTGGAA
    CAGGAGCTCC AGAAGTACAA GGCCCTCTAT GGAGACGTGG ACAGCCCCCT CCCCACGGGG
    GAAGCCGGGG GGCCGCCCAG CACCAGGGAG GCTGAGCTGA AGCTGCGCTT GAAGTTGGTG
    GAGGAAGAGG CCAACATCTT GGGCCGGAAG ATTGTGGAAC TGGAGGTGGA GAACCGAGGC
    CTCAAAGCAG AAATGGAGGA CATGAGGCTC CAGTACGAGC GAGAGGGTAC AGGCCGGGAC
    CACATCACAG GCATTCCTAC CTCACCCTTC GGTGACTCCC TGGAGTCCTC GACGGAGCTC
    CGCCGGCACC TGCAGTTCGT GGAGGAGGAA GCGGAGCTGC TCCGGAGGTC CATATCTGAG
    ATCGAAGACC ACAACCGGCA GCTGACCCAT GAGCTGAACA AGTTCAAGTT TGAGCCTCGG
    CCGGAGCCAG GGTGGCTGGC GGATGGCGCG GGCAAGGGCG TGGGCAGCGG GGCCCCTCTG
    CAGGAGGAGC TCAAGTCGGC ACGGCTACAG ATCAATGAGC TCAGTGGGAA GGTGCTGAAG
    CTGCAGTATG AGAACCGGGT GCTACTGTCC AATGTGCAGC GCTATGACCT GGCTGCCCAC
    CTAGGCCTGC GTGCCCCCAG CCCCCGGGAC AGCGACGCAG ACAGCGACCA GGGCAAGAAG
    GAGAGCGATG GGGAGGAAGG CCGCCAGCCC CAGCCCAAGC GGGAGGGCCC TATTGGCGGG
    GAGAGCGACT CAGAGGAGAT GTGCGAGAAG ACGTCGGGCT TCGGCAGCGG AAAGCCATCG
    GAGGCGAGCG AGCCATGTCC CGCCGAGCTC CTGCGGGCCA GGGAGGACAC CGAGTGCCTG
    GCGAGCATAA AGCACGAGGC TGAGCGCCTG GAGCGAACAG TGGAGCGCCT CATCACGGAC
    ACCGCGGGCT TCGGGGACGA TGTGGGGCTG CTGGGCAGTG GCGAGCCCTT GCCAGGGCCC
    GACATCCAGG GGGCGGGGGG GACGGGCGAG GGGGGCAAGA AGGAGCCCCC ACTGCTGGGG
    ACCATCAACT CGAGGATGAA GGCTTTCAGG AAGGAGCTGC AGGCCTTCCT GGAGCAGGTG
    AACCGAATCG GGGATGGCCT GCGGGAGCGC CTGGAAGGCC TGTCCCCCCT GCCTCACCTC
    ACAGAGTCTT CCAGCTTTCT CTCCACCGTC ACTTCCGTGT CCCGGGACTC CCCCGTCGGG
    AACCTGGGGA AGGAGCTGGT CCCGGACCTG CAGTCCAAAC TGAGAGGTCA GCTGGAGTGG
    CAGCCGGGGC AGGAGTGCGG GGACGAGCCA GAGCAGCTGC ACCGCCGCGC TGATGGGGAC
    GTCGGGAGCT GCCGGCAGGG AGGCCAGAGC TGCTTCAGTC TAGAGAGTGT ATCGCTAGAG
    GAGCTGCAGG GTCAGCTTGC GCAGGCGACC AGACTGCATC AAGAGGAGAC AGAGAAATTT
    ACAAACAAGA TTCGGAAGAT GGAGGAGGAG CACCTGTACG CCTTGAGGTG GAAAGAACTG
    GAAATGCACA GCCTGGCTTT GCAGAACACC CTCCATGAGC AAACCTGGAG TGATGAGAGG
    AACCTGATGC AGCAGGAGCT CCGGTCTATG AAGCAGAACA TTTTCCTTTT CTACGTCAAA
    CTCAGATGGC TGCTGAGGCA CTGGCGACAA GGGAAGCAGA TGGAGGAAGA AGGCGAGGAT
    TTCACTGAGA GCGAGCATCC AGAGACCCTC CCCAGGCTTG GTGAGCTTGG AGTGCAGGGG
    GACCCGAAGG GGGACGGCCC AGACCAAGAT GACAACGGTC CGGGCTGCGG CTTTCCGCTG
    GGAGAGCATC CTCCACACTC CCCTGTGCAG ATTGGTGACC ATGGATCGAG ACTACAGCCT
    ACACATGGGA GACAGCTCCA CAGGCAGGTG GTGGAAAACC AGCAGCTGTT CAGTGCACTC
    AAGAGCCTGC TGGAGGACCT GCGCGCCGAG CTGCGGGAGG ACGAGCATGC ACGGCGGCGG
    CTGCAGCAGC AGTACGCCAG CGACAAAGCC GCCTGGGACG TGGAGTGGGC TGCACTTACC
    TGCCGCCTGG AACAGGTGCC TGCCTGCGGG GTCTGTCCTG GGTCTCCCCA GCTCCTCCTC
    TGCCATCTGG GCCAGCTGGA AGAGAAGACT GAGAAGAATT TGGGAGAAGC ATGCTCTTCC
    ACCGACAGCA AAGGGGCATT CAAGAAGGAA AGAGAGATGC ATCAGAAGCT CCTCACTGAC
    AGTCATGGCC TGGTCATGGA CCTGCGCTGG CAGATCCATC ACAGTGAGAA GAACTGGAGC
    CGGGAGAAGG TGGAACTTCT TAGCCGTCTA GACAGAGACC GGCAGGAATG GGAGCAGCAG
    AAGAAGGAGC TCTTGTGGAG AATAGAACAG TTGCAGAAAG AAAACAGTCC CCGGAGAAGT
    GGCAGTTTCC TCTGTGATCA AAAAGAAGGC ACCATCCGCC CCTTTGCCCA TCCAGGAAGC
    CCCCGAATGC CCCGTCCCTT GGGGATGTGG TCCTGTGCAG ACACCGACTC CACCCCATTC
    GAAGACCGGC CGCTGTCCAA GCTGAAGGAA TCAGACAGAT GTTCAGCACG GGAAAACCTC
    TACTTGGACG CCTTGTCTCT AGATGACGAG CCAGAGGAGC CTCCAGCCCG AGAGCCCCAG
    CGGGAGTTCA GGAACTGCCT CCCCCAGGAA GAAGAAAATC ACAAGGGAAA TCTTCAAAGG
    GCCGTGTCTG TGTCCTCCAT GTCTGAGTTC CAGCGTCTGA TGGACGTTTC TCCCTTCCTG
    CCGGAGAAGG GCGTCCCCGC CACCAGCAGC AAGGAGGACA TCACTCCGCC CCTGTCTCCT
    GATGATCTGA AGTACATCGA GGAGTTCAAC AGCAAGAACT GGGACGAGAG GCCCCCGGAG
    CCCTGGGCAG ACAGGACCGA GGGGGGGCGG GCAGGGAGCG AGGCTGGTGC AGAGCCTTTC
    CCTGACTCCT CCTGGTACCT CACCACGAGT GTCACCATGA CTACGGACAC CATGACCAGC
    CCAGAGCACT GCCAGAAGCA GCCCCTTCGG AGCCCCATGT GCGCGGAGCA GGCCGGGGTG
    CGGGTGCTGC ACAGCCCGCC CGCGGTCCGT AGGATGGATG GCATCGTGGT GGCGGGCAGT
    GAGGGCAAGG GCCGGGCCGA GCCCGAGTGC CCGTTTCCAA CTAGCAGAGC CAGGGGGGGC
    ACTGGAGACA CAAAAGGGGG TCCCTCAGAA CACGTGCTCA GCAGGTGGCC TTGCGCCCCC
    TCCAGGCACC CCCGAGACTA TGTGGAGGGA GGGCTGCGCC CCCTCGAAAG TGCCCTCTGC
    GCCCCCCTGG GTTTTGCCTC CTCGCTGCAC GGCCTGCAGA TGTCCAAGAA CATGAGTGAT
    GACATGAAGG AGGTGGCCTT CTCGGTCAGG AACGCCATCT GCTCTGGCCC CCCCAAGCCT
    CCGCTCAAGG ACATGGCCTG CCAGACCAAT GGGTCCCGGA CGACGGGAAC ACAGACCGTC
    CAGACCATCA CCATTGGCCT GCAGACCGAA GCCCTGCGTG GCAGCGCCGT CACCAGCAGC
    CCCCACAAGT GTCTCACACC CAAGGCCGGG GGCGGGGCCA CGCCCGCGTC ATCTCCTTCC
    CGTGGCCTTC GGAGCCGGCA GATGGCCCCC GCCATCGAGA AGGTGCAGGC CAAGTTCGAA
    CGCACGTGCT GCTCCCCCAA GTACGGGTCT CCCAAGCTGC AGAGGAAGCC CCTCCCCAAA
    GCTGAGCCGC CCAACAGCAG GACCTCCCTG GGGCTGGCCC CAAAGGGGTG CAGCGAGTCC
    GCCTGGGCCC GTTCCACCAC CACGAGGGAG AGCCCGGTGC ACACCGCCAT CAACGATGGC
    CTCTCCAGCC TCTTCAACAT CATCGACCAC AGCCCCGTGG TGCAGGACCC CTTCCAGAAG
    GGGACTCGGG CCGGGAGCCG ATCTCGCTCA GCAGAACCCC GGCCCGAGCT GGGCCCAGGC
    CAGGAAACGG GCCCCAGTTC CCGGGGCCGG TCCCCCAGCC CCATCGGGGC TGGCTCAGAG
    ACTTGGAGGG AGGAAGGGGG AGAGGGCACG CCCTTGAGGC AGGACTTATC CGCTCCCCCT
    GGTTACACGT TCACTGAGAA CGTAGCCCGG ATCCTCAACA AGAAGCTGTT GGAGCATGCC
    TTAAAGGAGG AGAGGAGGCA GGCCCCCCAC GGGCCCCCAA GTCTTAACAG TGACAGCCAC
    ATGGGAGAAA CCATCAAAGC TGAACCAGGG TCCATCGAGG AACTACCTTG TTCCGCACTA
    GCTCCATCCC TAGAACCCTG CTTCTCCAGG CCCGAGAGAC CAGCAAACCG TCGCCTTCCG
    TCCCGTTGGG CCTCACATTC CCCCACTGCC TCACTGGCTC AGTCACCCGG AGACCCAGCC
    TCCTTGGAGG AGCTCGGCGA TGAGGAGCCA CCGGGGGAGA AGACACACCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe289881
    Clone ID Related Accession (Same CDS sequence) XM_022421622.1
    Accession Version XM_022421622.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5019bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product microtubule cross-linking factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG
    CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG
    TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG
    CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC
    ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG
    GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT
    CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG
    TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC
    CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG
    CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG
    TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC
    CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC
    CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG
    GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG
    GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA
    TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC
    GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG
    GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG
    GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG
    ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT
    GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC
    TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG
    CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG
    TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG
    CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG AGTGTATCGC TAGAGGAGCT GCAGGGTCAG
    CTTGCGCAGG CGACCAGACT GCATCAAGAG GAGACAGAGA AATTTACAAA CAAGATTCGG
    AAGATGGAGG AGGAGCACCT GTACGCCTTG AGGTGGAAAG AACTGGAAAT GCACAGCCTG
    GCTTTGCAGA ACACCCTCCA TGAGCAAACC TGGAGTGATG AGAGGAACCT GATGCAGCAG
    GAGCTCCGGT CTATGAAGCA GAACATTTTC CTTTTCTACG TCAAACTCAG ATGGCTGCTG
    AGGCACTGGC GACAAGGGAA GCAGATGGAG GAAGAAGGCG AGGATTTCAC TGAGAGCGAG
    CATCCAGAGA CCCTCCCCAG GCTTGGTGAG CTTGGAGTGC AGGGGGACCC GAAGGGGGAC
    GGCCCAGACC AAGATGACAA CGGTCCGGGC TGCGGCTTTC CGCTGGGAGA GCATCCTCCA
    CACTCCCCTG TGCAGATTGG TGACCATGGA TCGAGACTAC AGCCTACACA TGGGAGACAG
    CTCCACAGGC AGGTGGTGGA AAACCAGCAG CTGTTCAGTG CACTCAAGAG CCTGCTGGAG
    GACCTGCGCG CCGAGCTGCG GGAGGACGAG CATGCACGGC GGCGGCTGCA GCAGCAGTAC
    GCCAGCGACA AAGCCGCCTG GGACGTGGAG TGGGCTGCAC TTACCTGCCG CCTGGAACAG
    GTGCCTGCCT GCGGGGTCTG TCCTGGGTCT CCCCAGCTCC TCCTCTGCCA TCTGGGCCAG
    CTGGAAGAGA AGACTGAGAA GAATTTGGGA GAAGCATGCT CTTCCACCGA CAGCAAAGGG
    GCATTCAAGA AGGAAAGAGA GATGCATCAG AAGCTCCTCA CTGACAGTCA TGGCCTGGTC
    ATGGACCTGC GCTGGCAGAT CCATCACAGT GAGAAGAACT GGAGCCGGGA GAAGGTGGAA
    CTTCTTAGCC GTCTAGACAG AGACCGGCAG GAATGGGAGC AGCAGAAGAA GGAGCTCTTG
    TGGAGAATAG AACAGTTGCA GAAAGAAAAC AGTCCCCGGA GAAGTGGCAG TTTCCTCTGT
    GATCAAAAAG AAGGCACCAT CCGCCCCTTT GCCCATCCAG GAAGCCCCCG AATGCCCCGT
    CCCTTGGGGA TGTGGTCCTG TGCAGACACC GACTCCACCC CATTCGAAGA CCGGCCGCTG
    TCCAAGCTGA AGGAATCAGA CAGATGTTCA GCACGGGAAA ACCTCTACTT GGACGCCTTG
    TCTCTAGATG ACGAGCCAGA GGAGCCTCCA GCCCGAGAGC CCCAGCGGGA GTTCAGGAAC
    TGCCTCCCCC AGGAAGAAGA AAATCACAAG GGAAATCTTC AAAGGGCCGT GTCTGTGTCC
    TCCATGTCTG AGTTCCAGCG TCTGATGGAC GTTTCTCCCT TCCTGCCGGA GAAGGGCGTC
    CCCGCCACCA GCAGCAAGGA GGACATCACT CCGCCCCTGT CTCCTGATGA TCTGAAGTAC
    ATCGAGGAGT TCAACAGCAA GAACTGGGAC GAGAGGCCCC CGGAGCCCTG GGCAGACAGG
    ACCGAGGGGG GGCGGGCAGG GAGCGAGGCT GGTGCAGAGC CTTTCCCTGA CTCCTCCTGG
    TACCTCACCA CGAGTGTCAC CATGACTACG GACACCATGA CCAGCCCAGA GCACTGCCAG
    AAGCAGCCCC TTCGGAGCCC CATGTGCGCG GAGCAGGCCG GGGTGCGGGT GCTGCACAGC
    CCGCCCGCGG TCCGTAGGAT GGATGGCATC GTGGTGGCGG GCAGTGAGGG CAAGGGCCGG
    GCCGAGCCCG AGTGCCCGTT TCCAACTAGC AGAGCCAGGG GGGGCACTGG AGACACAAAA
    GGGGGTCCCT CAGAACACGT GCTCAGCAGG TGGCCTTGCG CCCCCTCCAG GCACCCCCGA
    GACTATGTGG AGGGAGGGCT GCGCCCCCTC GAAAGTGCCC TCTGCGCCCC CCTGGGTTTT
    GCCTCCTCGC TGCACGGCCT GCAGATGTCC AAGAACATGA GTGATGACAT GAAGGAGGTG
    GCCTTCTCGG TCAGGAACGC CATCTGCTCT GGCCCCCCCA AGCCTCCGCT CAAGGACATG
    GCCTGCCAGA CCAATGGGTC CCGGACGACG GGAACACAGA CCGTCCAGAC CATCACCATT
    GGCCTGCAGA CCGAAGCCCT GCGTGGCAGC GCCGTCACCA GCAGCCCCCA CAAGTGTCTC
    ACACCCAAGG CCGGGGGCGG GGCCACGCCC GCGTCATCTC CTTCCCGTGG CCTTCGGAGC
    CGGCAGATGG CCCCCGCCAT CGAGAAGGTG CAGGCCAAGT TCGAACGCAC GTGCTGCTCC
    CCCAAGTACG GGTCTCCCAA GCTGCAGAGG AAGCCCCTCC CCAAAGCTGA GCCGCCCAAC
    AGCAGGACCT CCCTGGGGCT GGCCCCAAAG GGGTGCAGCG AGTCCGCCTG GGCCCGTTCC
    ACCACCACGA GGGAGAGCCC GGTGCACACC GCCATCAACG ATGGCCTCTC CAGCCTCTTC
    AACATCATCG ACCACAGCCC CGTGGTGCAG GACCCCTTCC AGAAGGGGAC TCGGGCCGGG
    AGCCGATCTC GCTCAGCAGA ACCCCGGCCC GAGCTGGGCC CAGGCCAGGA AACGGGCCCC
    AGTTCCCGGG GCCGGTCCCC CAGCCCCATC GGGGCTGGCT CAGAGACTTG GAGGGAGGAA
    GGGGGAGAGG GCACGCCCTT GAGGCAGGAC TTATCCGCTC CCCCTGGTTA CACGTTCACT
    GAGAACGTAG CCCGGATCCT CAACAAGAAG CTGTTGGAGC ATGCCTTAAA GGAGGAGAGG
    AGGCAGGCCC CCCACGGGCC CCCAAGTCTT AACAGTGACA GCCACATGGG AGAAACCATC
    AAAGCTGAAC CAGGGTCCAT CGAGGAACTA CCTTGTTCCG CACTAGCTCC ATCCCTAGAA
    CCCTGCTTCT CCAGGCCCGA GAGACCAGCA AACCGTCGCC TTCCGTCCCG TTGGGCCTCA
    CATTCCCCCA CTGCCTCACT GGCTCAGTCA CCCGGAGACC CAGCCTCCTT GGAGGAGCTC
    GGCGATGAGG AGCCACCGGG GGAGAAGACA CACCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022277330.1
    CDS117..5135
    Translation

    Target ORF information:

    RefSeq Version XM_022421622.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022421622.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG
    CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG
    TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG
    CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC
    ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG
    GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT
    CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG
    TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC
    CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG
    CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG
    TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC
    CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC
    CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG
    GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG
    GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA
    TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC
    GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG
    GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG
    GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG
    ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT
    GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC
    TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG
    CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG
    TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG
    CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG AGTGTATCGC TAGAGGAGCT GCAGGGTCAG
    CTTGCGCAGG CGACCAGACT GCATCAAGAG GAGACAGAGA AATTTACAAA CAAGATTCGG
    AAGATGGAGG AGGAGCACCT GTACGCCTTG AGGTGGAAAG AACTGGAAAT GCACAGCCTG
    GCTTTGCAGA ACACCCTCCA TGAGCAAACC TGGAGTGATG AGAGGAACCT GATGCAGCAG
    GAGCTCCGGT CTATGAAGCA GAACATTTTC CTTTTCTACG TCAAACTCAG ATGGCTGCTG
    AGGCACTGGC GACAAGGGAA GCAGATGGAG GAAGAAGGCG AGGATTTCAC TGAGAGCGAG
    CATCCAGAGA CCCTCCCCAG GCTTGGTGAG CTTGGAGTGC AGGGGGACCC GAAGGGGGAC
    GGCCCAGACC AAGATGACAA CGGTCCGGGC TGCGGCTTTC CGCTGGGAGA GCATCCTCCA
    CACTCCCCTG TGCAGATTGG TGACCATGGA TCGAGACTAC AGCCTACACA TGGGAGACAG
    CTCCACAGGC AGGTGGTGGA AAACCAGCAG CTGTTCAGTG CACTCAAGAG CCTGCTGGAG
    GACCTGCGCG CCGAGCTGCG GGAGGACGAG CATGCACGGC GGCGGCTGCA GCAGCAGTAC
    GCCAGCGACA AAGCCGCCTG GGACGTGGAG TGGGCTGCAC TTACCTGCCG CCTGGAACAG
    GTGCCTGCCT GCGGGGTCTG TCCTGGGTCT CCCCAGCTCC TCCTCTGCCA TCTGGGCCAG
    CTGGAAGAGA AGACTGAGAA GAATTTGGGA GAAGCATGCT CTTCCACCGA CAGCAAAGGG
    GCATTCAAGA AGGAAAGAGA GATGCATCAG AAGCTCCTCA CTGACAGTCA TGGCCTGGTC
    ATGGACCTGC GCTGGCAGAT CCATCACAGT GAGAAGAACT GGAGCCGGGA GAAGGTGGAA
    CTTCTTAGCC GTCTAGACAG AGACCGGCAG GAATGGGAGC AGCAGAAGAA GGAGCTCTTG
    TGGAGAATAG AACAGTTGCA GAAAGAAAAC AGTCCCCGGA GAAGTGGCAG TTTCCTCTGT
    GATCAAAAAG AAGGCACCAT CCGCCCCTTT GCCCATCCAG GAAGCCCCCG AATGCCCCGT
    CCCTTGGGGA TGTGGTCCTG TGCAGACACC GACTCCACCC CATTCGAAGA CCGGCCGCTG
    TCCAAGCTGA AGGAATCAGA CAGATGTTCA GCACGGGAAA ACCTCTACTT GGACGCCTTG
    TCTCTAGATG ACGAGCCAGA GGAGCCTCCA GCCCGAGAGC CCCAGCGGGA GTTCAGGAAC
    TGCCTCCCCC AGGAAGAAGA AAATCACAAG GGAAATCTTC AAAGGGCCGT GTCTGTGTCC
    TCCATGTCTG AGTTCCAGCG TCTGATGGAC GTTTCTCCCT TCCTGCCGGA GAAGGGCGTC
    CCCGCCACCA GCAGCAAGGA GGACATCACT CCGCCCCTGT CTCCTGATGA TCTGAAGTAC
    ATCGAGGAGT TCAACAGCAA GAACTGGGAC GAGAGGCCCC CGGAGCCCTG GGCAGACAGG
    ACCGAGGGGG GGCGGGCAGG GAGCGAGGCT GGTGCAGAGC CTTTCCCTGA CTCCTCCTGG
    TACCTCACCA CGAGTGTCAC CATGACTACG GACACCATGA CCAGCCCAGA GCACTGCCAG
    AAGCAGCCCC TTCGGAGCCC CATGTGCGCG GAGCAGGCCG GGGTGCGGGT GCTGCACAGC
    CCGCCCGCGG TCCGTAGGAT GGATGGCATC GTGGTGGCGG GCAGTGAGGG CAAGGGCCGG
    GCCGAGCCCG AGTGCCCGTT TCCAACTAGC AGAGCCAGGG GGGGCACTGG AGACACAAAA
    GGGGGTCCCT CAGAACACGT GCTCAGCAGG TGGCCTTGCG CCCCCTCCAG GCACCCCCGA
    GACTATGTGG AGGGAGGGCT GCGCCCCCTC GAAAGTGCCC TCTGCGCCCC CCTGGGTTTT
    GCCTCCTCGC TGCACGGCCT GCAGATGTCC AAGAACATGA GTGATGACAT GAAGGAGGTG
    GCCTTCTCGG TCAGGAACGC CATCTGCTCT GGCCCCCCCA AGCCTCCGCT CAAGGACATG
    GCCTGCCAGA CCAATGGGTC CCGGACGACG GGAACACAGA CCGTCCAGAC CATCACCATT
    GGCCTGCAGA CCGAAGCCCT GCGTGGCAGC GCCGTCACCA GCAGCCCCCA CAAGTGTCTC
    ACACCCAAGG CCGGGGGCGG GGCCACGCCC GCGTCATCTC CTTCCCGTGG CCTTCGGAGC
    CGGCAGATGG CCCCCGCCAT CGAGAAGGTG CAGGCCAAGT TCGAACGCAC GTGCTGCTCC
    CCCAAGTACG GGTCTCCCAA GCTGCAGAGG AAGCCCCTCC CCAAAGCTGA GCCGCCCAAC
    AGCAGGACCT CCCTGGGGCT GGCCCCAAAG GGGTGCAGCG AGTCCGCCTG GGCCCGTTCC
    ACCACCACGA GGGAGAGCCC GGTGCACACC GCCATCAACG ATGGCCTCTC CAGCCTCTTC
    AACATCATCG ACCACAGCCC CGTGGTGCAG GACCCCTTCC AGAAGGGGAC TCGGGCCGGG
    AGCCGATCTC GCTCAGCAGA ACCCCGGCCC GAGCTGGGCC CAGGCCAGGA AACGGGCCCC
    AGTTCCCGGG GCCGGTCCCC CAGCCCCATC GGGGCTGGCT CAGAGACTTG GAGGGAGGAA
    GGGGGAGAGG GCACGCCCTT GAGGCAGGAC TTATCCGCTC CCCCTGGTTA CACGTTCACT
    GAGAACGTAG CCCGGATCCT CAACAAGAAG CTGTTGGAGC ATGCCTTAAA GGAGGAGAGG
    AGGCAGGCCC CCCACGGGCC CCCAAGTCTT AACAGTGACA GCCACATGGG AGAAACCATC
    AAAGCTGAAC CAGGGTCCAT CGAGGAACTA CCTTGTTCCG CACTAGCTCC ATCCCTAGAA
    CCCTGCTTCT CCAGGCCCGA GAGACCAGCA AACCGTCGCC TTCCGTCCCG TTGGGCCTCA
    CATTCCCCCA CTGCCTCACT GGCTCAGTCA CCCGGAGACC CAGCCTCCTT GGAGGAGCTC
    GGCGATGAGG AGCCACCGGG GGAGAAGACA CACCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe289882
    Clone ID Related Accession (Same CDS sequence) XM_022421623.1
    Accession Version XM_022421623.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product microtubule cross-linking factor 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    CAGAGTGGTG GAACCGAGCG CCTTGGAAAC TGCAGGTCTA CAACCAAAAC TCAAAGGAAA
    CTGGCACCCC GGCGCAAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA
    GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG
    GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG
    GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG
    GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA
    GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG
    GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG
    CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT
    GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT
    CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT
    CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG
    AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC
    CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG
    AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC
    GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA
    TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC
    CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG
    GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG
    CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG
    GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG
    GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC
    CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC
    GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGTCCA AACTGAGAGG TCAGCTGGAG
    TGGCAGCCGG GGCAGGAGTG CGGGGACGAG CCAGAGCAGC TGCACCGCCG CGCTGATGGG
    GACGTCGGGA GCTGCCGGCA GGGAGGCCAG AGCTGCTTCA GTCTAGAGAT GGAGGAGGAG
    CACCTGTACG CCTTGAGGTG GAAAGAACTG GAAATGCACA GCCTGGCTTT GCAGAACACC
    CTCCATGAGC AAACCTGGAG TGATGAGAGG AACCTGATGC AGCAGGAGCT CCGGTCTATG
    AAGCAGAACA TTTTCCTTTT CTACGTCAAA CTCAGATGGC TGCTGAGGCA CTGGCGACAA
    GGGAAGCAGA TGGAGGAAGA AGGCGAGGAT TTCACTGAGA GCGAGCATCC AGAGACCCTC
    CCCAGGCTTG GTGAGCTTGG AGTGCAGGGG GACCCGAAGG GGGACGGCCC AGACCAAGAT
    GACAACGGTC CGGGCTGCGG CTTTCCGCTG GGAGAGCATC CTCCACACTC CCCTGTGCAG
    ATTGGTGACC ATGGATCGAG ACTACAGCCT ACACATGGGA GACAGCTCCA CAGGCAGGTG
    GTGGAAAACC AGCAGCTGTT CAGTGCACTC AAGAGCCTGC TGGAGGACCT GCGCGCCGAG
    CTGCGGGAGG ACGAGCATGC ACGGCGGCGG CTGCAGCAGC AGTACGCCAG CGACAAAGCC
    GCCTGGGACG TGGAGTGGGC TGCACTTACC TGCCGCCTGG AACAGGTGCC TGCCTGCGGG
    GTCTGTCCTG GGTCTCCCCA GCTCCTCCTC TGCCATCTGG GCCAGCTGGA AGAGAAGACT
    GAGAAGAATT TGGGAGAAGC ATGCTCTTCC ACCGACAGCA AAGGGGCATT CAAGAAGGAA
    AGAGAGATGC ATCAGAAGCT CCTCACTGAC AGTCATGGCC TGGTCATGGA CCTGCGCTGG
    CAGATCCATC ACAGTGAGAA GAACTGGAGC CGGGAGAAGG TGGAACTTCT TAGCCGTCTA
    GACAGAGACC GGCAGGAATG GGAGCAGCAG AAGAAGGAGC TCTTGTGGAG AATAGAACAG
    TTGCAGAAAG AAAACAGTCC CCGGAGAAGT GGCAGTTTCC TCTGTGATCA AAAAGAAGGC
    ACCATCCGCC CCTTTGCCCA TCCAGGAAGC CCCCGAATGC CCCGTCCCTT GGGGATGTGG
    TCCTGTGCAG ACACCGACTC CACCCCATTC GAAGACCGGC CGCTGTCCAA GCTGAAGGAA
    TCAGACAGAT GTTCAGCACG GGAAAACCTC TACTTGGACG CCTTGTCTCT AGATGACGAG
    CCAGAGGAGC CTCCAGCCCG AGAGCCCCAG CGGGAGTTCA GGAACTGCCT CCCCCAGGAA
    GAAGAAAATC ACAAGGGAAA TCTTCAAAGG GCCGTGTCTG TGTCCTCCAT GTCTGAGTTC
    CAGCGTCTGA TGGACGTTTC TCCCTTCCTG CCGGAGAAGG GCGTCCCCGC CACCAGCAGC
    AAGGAGGACA TCACTCCGCC CCTGTCTCCT GATGATCTGA AGTACATCGA GGAGTTCAAC
    AGCAAGAACT GGGACGAGAG GCCCCCGGAG CCCTGGGCAG ACAGGACCGA GGGGGGGCGG
    GCAGGGAGCG AGGCTGGTGC AGAGCCTTTC CCTGACTCCT CCTGGTACCT CACCACGAGT
    GTCACCATGA CTACGGACAC CATGACCAGC CCAGAGCACT GCCAGAAGCA GCCCCTTCGG
    AGCCCCATGT GCGCGGAGCA GGCCGGGGTG CGGGTGCTGC ACAGCCCGCC CGCGGTCCGT
    AGGATGGATG GCATCGTGGT GGCGGGCAGT GAGGGCAAGG GCCGGGCCGA GCCCGAGTGC
    CCGTTTCCAA CTAGCAGAGC CAGGGGGGGC ACTGGAGACA CAAAAGGGGG TCCCTCAGAA
    CACGTGCTCA GCAGGTGGCC TTGCGCCCCC TCCAGGCACC CCCGAGACTA TGTGGAGGGA
    GGGCTGCGCC CCCTCGAAAG TGCCCTCTGC GCCCCCCTGG GTTTTGCCTC CTCGCTGCAC
    GGCCTGCAGA TGTCCAAGAA CATGAGTGAT GACATGAAGG AGGTGGCCTT CTCGGTCAGG
    AACGCCATCT GCTCTGGCCC CCCCAAGCCT CCGCTCAAGG ACATGGCCTG CCAGACCAAT
    GGGTCCCGGA CGACGGGAAC ACAGACCGTC CAGACCATCA CCATTGGCCT GCAGACCGAA
    GCCCTGCGTG GCAGCGCCGT CACCAGCAGC CCCCACAAGT GTCTCACACC CAAGGCCGGG
    GGCGGGGCCA CGCCCGCGTC ATCTCCTTCC CGTGGCCTTC GGAGCCGGCA GATGGCCCCC
    GCCATCGAGA AGGTGCAGGC CAAGTTCGAA CGCACGTGCT GCTCCCCCAA GTACGGGTCT
    CCCAAGCTGC AGAGGAAGCC CCTCCCCAAA GCTGAGCCGC CCAACAGCAG GACCTCCCTG
    GGGCTGGCCC CAAAGGGGTG CAGCGAGTCC GCCTGGGCCC GTTCCACCAC CACGAGGGAG
    AGCCCGGTGC ACACCGCCAT CAACGATGGC CTCTCCAGCC TCTTCAACAT CATCGACCAC
    AGCCCCGTGG TGCAGGACCC CTTCCAGAAG GGGACTCGGG CCGGGAGCCG ATCTCGCTCA
    GCAGAACCCC GGCCCGAGCT GGGCCCAGGC CAGGAAACGG GCCCCAGTTC CCGGGGCCGG
    TCCCCCAGCC CCATCGGGGC TGGCTCAGAG ACTTGGAGGG AGGAAGGGGG AGAGGGCACG
    CCCTTGAGGC AGGACTTATC CGCTCCCCCT GGTTACACGT TCACTGAGAA CGTAGCCCGG
    ATCCTCAACA AGAAGCTGTT GGAGCATGCC TTAAAGGAGG AGAGGAGGCA GGCCCCCCAC
    GGGCCCCCAA GTCTTAACAG TGACAGCCAC ATGGGAGAAA CCATCAAAGC TGAACCAGGG
    TCCATCGAGG AACTACCTTG TTCCGCACTA GCTCCATCCC TAGAACCCTG CTTCTCCAGG
    CCCGAGAGAC CAGCAAACCG TCGCCTTCCG TCCCGTTGGG CCTCACATTC CCCCACTGCC
    TCACTGGCTC AGTCACCCGG AGACCCAGCC TCCTTGGAGG AGCTCGGCGA TGAGGAGCCA
    CCGGGGGAGA AGACACACCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022277331.1
    CDS117..5120
    Translation

    Target ORF information:

    RefSeq Version XM_022421623.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022421623.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    CAGAGTGGTG GAACCGAGCG CCTTGGAAAC TGCAGGTCTA CAACCAAAAC TCAAAGGAAA
    CTGGCACCCC GGCGCAAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA
    GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG
    GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG
    GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG
    GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA
    GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG
    GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG
    CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT
    GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT
    CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT
    CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG
    AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC
    CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG
    AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC
    GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA
    TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC
    CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG
    GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG
    CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG
    GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG
    GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC
    CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC
    GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGTCCA AACTGAGAGG TCAGCTGGAG
    TGGCAGCCGG GGCAGGAGTG CGGGGACGAG CCAGAGCAGC TGCACCGCCG CGCTGATGGG
    GACGTCGGGA GCTGCCGGCA GGGAGGCCAG AGCTGCTTCA GTCTAGAGAT GGAGGAGGAG
    CACCTGTACG CCTTGAGGTG GAAAGAACTG GAAATGCACA GCCTGGCTTT GCAGAACACC
    CTCCATGAGC AAACCTGGAG TGATGAGAGG AACCTGATGC AGCAGGAGCT CCGGTCTATG
    AAGCAGAACA TTTTCCTTTT CTACGTCAAA CTCAGATGGC TGCTGAGGCA CTGGCGACAA
    GGGAAGCAGA TGGAGGAAGA AGGCGAGGAT TTCACTGAGA GCGAGCATCC AGAGACCCTC
    CCCAGGCTTG GTGAGCTTGG AGTGCAGGGG GACCCGAAGG GGGACGGCCC AGACCAAGAT
    GACAACGGTC CGGGCTGCGG CTTTCCGCTG GGAGAGCATC CTCCACACTC CCCTGTGCAG
    ATTGGTGACC ATGGATCGAG ACTACAGCCT ACACATGGGA GACAGCTCCA CAGGCAGGTG
    GTGGAAAACC AGCAGCTGTT CAGTGCACTC AAGAGCCTGC TGGAGGACCT GCGCGCCGAG
    CTGCGGGAGG ACGAGCATGC ACGGCGGCGG CTGCAGCAGC AGTACGCCAG CGACAAAGCC
    GCCTGGGACG TGGAGTGGGC TGCACTTACC TGCCGCCTGG AACAGGTGCC TGCCTGCGGG
    GTCTGTCCTG GGTCTCCCCA GCTCCTCCTC TGCCATCTGG GCCAGCTGGA AGAGAAGACT
    GAGAAGAATT TGGGAGAAGC ATGCTCTTCC ACCGACAGCA AAGGGGCATT CAAGAAGGAA
    AGAGAGATGC ATCAGAAGCT CCTCACTGAC AGTCATGGCC TGGTCATGGA CCTGCGCTGG
    CAGATCCATC ACAGTGAGAA GAACTGGAGC CGGGAGAAGG TGGAACTTCT TAGCCGTCTA
    GACAGAGACC GGCAGGAATG GGAGCAGCAG AAGAAGGAGC TCTTGTGGAG AATAGAACAG
    TTGCAGAAAG AAAACAGTCC CCGGAGAAGT GGCAGTTTCC TCTGTGATCA AAAAGAAGGC
    ACCATCCGCC CCTTTGCCCA TCCAGGAAGC CCCCGAATGC CCCGTCCCTT GGGGATGTGG
    TCCTGTGCAG ACACCGACTC CACCCCATTC GAAGACCGGC CGCTGTCCAA GCTGAAGGAA
    TCAGACAGAT GTTCAGCACG GGAAAACCTC TACTTGGACG CCTTGTCTCT AGATGACGAG
    CCAGAGGAGC CTCCAGCCCG AGAGCCCCAG CGGGAGTTCA GGAACTGCCT CCCCCAGGAA
    GAAGAAAATC ACAAGGGAAA TCTTCAAAGG GCCGTGTCTG TGTCCTCCAT GTCTGAGTTC
    CAGCGTCTGA TGGACGTTTC TCCCTTCCTG CCGGAGAAGG GCGTCCCCGC CACCAGCAGC
    AAGGAGGACA TCACTCCGCC CCTGTCTCCT GATGATCTGA AGTACATCGA GGAGTTCAAC
    AGCAAGAACT GGGACGAGAG GCCCCCGGAG CCCTGGGCAG ACAGGACCGA GGGGGGGCGG
    GCAGGGAGCG AGGCTGGTGC AGAGCCTTTC CCTGACTCCT CCTGGTACCT CACCACGAGT
    GTCACCATGA CTACGGACAC CATGACCAGC CCAGAGCACT GCCAGAAGCA GCCCCTTCGG
    AGCCCCATGT GCGCGGAGCA GGCCGGGGTG CGGGTGCTGC ACAGCCCGCC CGCGGTCCGT
    AGGATGGATG GCATCGTGGT GGCGGGCAGT GAGGGCAAGG GCCGGGCCGA GCCCGAGTGC
    CCGTTTCCAA CTAGCAGAGC CAGGGGGGGC ACTGGAGACA CAAAAGGGGG TCCCTCAGAA
    CACGTGCTCA GCAGGTGGCC TTGCGCCCCC TCCAGGCACC CCCGAGACTA TGTGGAGGGA
    GGGCTGCGCC CCCTCGAAAG TGCCCTCTGC GCCCCCCTGG GTTTTGCCTC CTCGCTGCAC
    GGCCTGCAGA TGTCCAAGAA CATGAGTGAT GACATGAAGG AGGTGGCCTT CTCGGTCAGG
    AACGCCATCT GCTCTGGCCC CCCCAAGCCT CCGCTCAAGG ACATGGCCTG CCAGACCAAT
    GGGTCCCGGA CGACGGGAAC ACAGACCGTC CAGACCATCA CCATTGGCCT GCAGACCGAA
    GCCCTGCGTG GCAGCGCCGT CACCAGCAGC CCCCACAAGT GTCTCACACC CAAGGCCGGG
    GGCGGGGCCA CGCCCGCGTC ATCTCCTTCC CGTGGCCTTC GGAGCCGGCA GATGGCCCCC
    GCCATCGAGA AGGTGCAGGC CAAGTTCGAA CGCACGTGCT GCTCCCCCAA GTACGGGTCT
    CCCAAGCTGC AGAGGAAGCC CCTCCCCAAA GCTGAGCCGC CCAACAGCAG GACCTCCCTG
    GGGCTGGCCC CAAAGGGGTG CAGCGAGTCC GCCTGGGCCC GTTCCACCAC CACGAGGGAG
    AGCCCGGTGC ACACCGCCAT CAACGATGGC CTCTCCAGCC TCTTCAACAT CATCGACCAC
    AGCCCCGTGG TGCAGGACCC CTTCCAGAAG GGGACTCGGG CCGGGAGCCG ATCTCGCTCA
    GCAGAACCCC GGCCCGAGCT GGGCCCAGGC CAGGAAACGG GCCCCAGTTC CCGGGGCCGG
    TCCCCCAGCC CCATCGGGGC TGGCTCAGAG ACTTGGAGGG AGGAAGGGGG AGAGGGCACG
    CCCTTGAGGC AGGACTTATC CGCTCCCCCT GGTTACACGT TCACTGAGAA CGTAGCCCGG
    ATCCTCAACA AGAAGCTGTT GGAGCATGCC TTAAAGGAGG AGAGGAGGCA GGCCCCCCAC
    GGGCCCCCAA GTCTTAACAG TGACAGCCAC ATGGGAGAAA CCATCAAAGC TGAACCAGGG
    TCCATCGAGG AACTACCTTG TTCCGCACTA GCTCCATCCC TAGAACCCTG CTTCTCCAGG
    CCCGAGAGAC CAGCAAACCG TCGCCTTCCG TCCCGTTGGG CCTCACATTC CCCCACTGCC
    TCACTGGCTC AGTCACCCGG AGACCCAGCC TCCTTGGAGG AGCTCGGCGA TGAGGAGCCA
    CCGGGGGAGA AGACACACCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe289883
    Clone ID Related Accession (Same CDS sequence) XM_022421624.1
    Accession Version XM_022421624.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product microtubule cross-linking factor 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    CAGAGTGGTG GAACCGAGCG CCTTGGAAAC TGCAGGTCTA CAACCAAAAC TCAAAGGAAA
    CTGGCACCCC GGCGCAAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA
    GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG
    GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG
    GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG
    GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA
    GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG
    GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG
    CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT
    GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT
    CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT
    CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG
    AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC
    CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG
    AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC
    GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA
    TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC
    CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG
    GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG
    CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG
    GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG
    GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC
    CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC
    GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGTCCA AACTGAGAGG TCAGCTGGAG
    TGGCAGCCGG GGCAGGAGTG CGGGGACGAG CCAGAGCAGC TGCACCGCCG CGCTGATGGG
    GACGTCGGGA GCTGCCGGCA GGGAGGCCAG AGCTGCTTCA GTCTAGAGAG TGTATCGCTA
    GAGGAGCTGC AGGGTCAGCT TGCGCAGGCG ACCAGACTGC ATCAAGAGGA GACAGAGAAA
    TTTACAAACA AGATTCGGAA GATGGAGGAG GAGCACCTGT ACGCCTTGAG GTGGAAAGAA
    CTGGAAATGC ACAGCCTGGC TTTGCAGAAC ACCCTCCATG AGCAAACCTG GAGTGATGAG
    AGGAACCTGA TGCAGCAGGA GCTCCGGTCT ATGAAGCAGA ACATTTTCCT TTTCTACGTC
    AAACTCAGAT GGCTGCTGAG GCACTGGCGA CAAGGGAAGC AGATGGAGGA AGAAGGCGAG
    GATTTCACTG AGAGCGAGCA TCCAGAGACC CTCCCCAGGC TTGGTGAGCT TGGAGTGCAG
    GGGGACCCGA AGGGGGACGG CCCAGACCAA GATGACAACG GTCCGGGCTG CGGCTTTCCG
    CTGGGAGAGC ATCCTCCACA CTCCCCTGTG CAGATTGGTG ACCATGGATC GAGACTACAG
    CCTACACATG GGAGACAGCT CCACAGGCAG GTGGTGGAAA ACCAGCAGCT GTTCAGTGCA
    CTCAAGAGCC TGCTGGAGGA CCTGCGCGCC GAGCTGCGGG AGGACGAGCA TGCACGGCGG
    CGGCTGCAGC AGCAGTACGC CAGCGACAAA GCCGCCTGGG ACGTGGAGTG GGCTGCACTT
    ACCTGCCGCC TGGAACAGGT GCCTGCCTGC GGGGTCTGTC CTGGGTCTCC CCAGCTCCTC
    CTCTGCCATC TGGGCCAGCT GGAAGAGAAG ACTGAGAAGA ATTTGGGAGA AGCATGCTCT
    TCCACCGACA GCAAAGGGGC ATTCAAGAAG GAAAGAGAGA TGCATCAGAA GCTCCTCACT
    GACAGTCATG GCCTGGTCAT GGACCTGCGC TGGCAGATCC ATCACAGTGA GAAGAACTGG
    AGCCGGGAGA AGGTGGAACT TCTTAGCCGT CTAGACAGAG ACCGGCAGGA ATGGGAGCAG
    CAGAAGAAGG AGCTCTTGTG GAGAATAGAA CAGTTGCAGA AAGAAAACAG TCCCCGGAGA
    AGTGGCAGTT TCCTCTGTGA TCAAAAAGAA GGCACCATCC GCCCCTTTGC CCATCCAGGA
    AGCCCCCGAA TGCCCCGTCC CTTGGGGATG TGGTCCTGTG CAGACACCGA CTCCACCCCA
    TTCGAAGACC GGCCGCTGTC CAAGCTGAAG GAATCAGACA GATGTTCAGC ACGGGAAAAC
    CTCTACTTGG ACGCCTTGTC TCTAGATGAC GAGCCAGAGG AGCCTCCAGC CCGAGAGCCC
    CAGCGGGAGT TCAGGAACTG CCTCCCCCAG GAAGAAGAAA ATCACAAGGG AAATCTTCAA
    AGGGCCGTGT CTGTGTCCTC CATGTCTGAG TTCCAGCGTC TGATGGACGT TTCTCCCTTC
    CTGCCGGAGA AGGGCGTCCC CGCCACCAGC AGCAAGGAGG ACATCACTCC GCCCCTGTCT
    CCTGATGATC TGAAGTACAT CGAGGAGTTC AACAGCAAGA ACTGGGACGA GAGGCCCCCG
    GAGCCCTGGG CAGACAGGAC CGAGGGGGGG CGGGCAGGGA GCGAGGCTGG TGCAGAGCCT
    TTCCCTGACT CCTCCTGGTA CCTCACCACG AGTGTCACCA TGACTACGGA CACCATGACC
    AGCCCAGAGC ACTGCCAGAA GCAGCCCCTT CGGAGCCCCA TGTGCGCGGA GCAGGCCGGG
    GTGCGGGTGC TGCACAGCCC GCCCGCGGTC CGTAGGATGG ATGGCATCGT GGTGGCGGGC
    AGTGAGGGCA AGGGCCGGGC CGAGCCCGAG TGCCCGTTTC CAACTAGCAG AGCCAGGGGG
    GGCACTGGAG ACACAAAAGG GGGTCCCTCA GAACACGTGC TCAGCAGGTG GCCTTGCGCC
    CCCTCCAGGC ACCCCCGAGA CTATGTGGAG GGAGGGCTGC GCCCCCTCGA AAGTGCCCTC
    TGCGCCCCCC TGGGTTTTGC CTCCTCGCTG CACGGCCTGC AGATGTCCAA GAACATGAGT
    GATGACATGA AGGAGGTGGC CTTCTCGGTC AGGAACGCCA TCTGCTCTGG CCCCCCCAAG
    CCTCCGCTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGACGGG AACACAGACC
    GTCCAGACCA TCACCATTGG CCTGCAGACC GAAGCCCTGC GTGGCAGCGC CGTCACCAGC
    AGCCCCCACA AGTGTCTCAC ACCCAAGGCC GGGGGCGGGG CCACGCCCGC GTCATCTCCT
    TCCCGTGGCC TTCGGAGCCG GCAGATGGCC CCCGCCATCG AGAAGGTGCA GGCCAAGTTC
    GAACGCACGT GCTGCTCCCC CAAGTACGGG TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC
    AAAGCTGAGC CGCCCAACAG CAGGACCTCC CTGGGGCTGG CCCCAAAGGG GTGCAGCGAG
    TCCGCCTGGG CCCGTTCCAC CACCACGAGG GAGAGCCCGG TGCACACCGC CATCAACGAT
    GGCCTCTCCA GCCTCTTCAA CATCATCGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG
    AAGGGGACTC GGGCCGGGAG CCGATCTCGC TCAGCAGAAC CCCGGCCCGA GCTGGGCCCA
    GGCCAGGAAA CGGGCCCCAG TTCCCGGGGC CGGTCCCCCA GCCCCATCGG GGCTGGCTCA
    GAGACTTGGA GGGAGGAAGG GGGAGAGGGC ACGCCCTTGA GGCAGGACTT ATCCGCTCCC
    CCTGGTTACA CGTTCACTGA GAACGTAGCC CGGATCCTCA ACAAGAAGCT GTTGGAGCAT
    GCCTTAAAGG AGGAGAGGAG GCAGGCCCCC CACGGGCCCC CAAGTCTTAA CAGTGACAGC
    CACATGGGAG AAACCATCAA AGCTGAACCA GGGTCCATCG AGAACCAAAC TGTCTTACTA
    ACTGCCCCCT GGGAACTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022277332.1
    CDS119..5059
    Translation

    Target ORF information:

    RefSeq Version XM_022421624.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022421624.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    CAGAGTGGTG GAACCGAGCG CCTTGGAAAC TGCAGGTCTA CAACCAAAAC TCAAAGGAAA
    CTGGCACCCC GGCGCAAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA
    GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG
    GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG
    GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG
    GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA
    GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG
    GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG
    CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT
    GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT
    CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT
    CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG
    AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC
    CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG
    AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC
    GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA
    TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC
    CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG
    GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG
    CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG
    GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG
    GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC
    CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC
    GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGTCCA AACTGAGAGG TCAGCTGGAG
    TGGCAGCCGG GGCAGGAGTG CGGGGACGAG CCAGAGCAGC TGCACCGCCG CGCTGATGGG
    GACGTCGGGA GCTGCCGGCA GGGAGGCCAG AGCTGCTTCA GTCTAGAGAG TGTATCGCTA
    GAGGAGCTGC AGGGTCAGCT TGCGCAGGCG ACCAGACTGC ATCAAGAGGA GACAGAGAAA
    TTTACAAACA AGATTCGGAA GATGGAGGAG GAGCACCTGT ACGCCTTGAG GTGGAAAGAA
    CTGGAAATGC ACAGCCTGGC TTTGCAGAAC ACCCTCCATG AGCAAACCTG GAGTGATGAG
    AGGAACCTGA TGCAGCAGGA GCTCCGGTCT ATGAAGCAGA ACATTTTCCT TTTCTACGTC
    AAACTCAGAT GGCTGCTGAG GCACTGGCGA CAAGGGAAGC AGATGGAGGA AGAAGGCGAG
    GATTTCACTG AGAGCGAGCA TCCAGAGACC CTCCCCAGGC TTGGTGAGCT TGGAGTGCAG
    GGGGACCCGA AGGGGGACGG CCCAGACCAA GATGACAACG GTCCGGGCTG CGGCTTTCCG
    CTGGGAGAGC ATCCTCCACA CTCCCCTGTG CAGATTGGTG ACCATGGATC GAGACTACAG
    CCTACACATG GGAGACAGCT CCACAGGCAG GTGGTGGAAA ACCAGCAGCT GTTCAGTGCA
    CTCAAGAGCC TGCTGGAGGA CCTGCGCGCC GAGCTGCGGG AGGACGAGCA TGCACGGCGG
    CGGCTGCAGC AGCAGTACGC CAGCGACAAA GCCGCCTGGG ACGTGGAGTG GGCTGCACTT
    ACCTGCCGCC TGGAACAGGT GCCTGCCTGC GGGGTCTGTC CTGGGTCTCC CCAGCTCCTC
    CTCTGCCATC TGGGCCAGCT GGAAGAGAAG ACTGAGAAGA ATTTGGGAGA AGCATGCTCT
    TCCACCGACA GCAAAGGGGC ATTCAAGAAG GAAAGAGAGA TGCATCAGAA GCTCCTCACT
    GACAGTCATG GCCTGGTCAT GGACCTGCGC TGGCAGATCC ATCACAGTGA GAAGAACTGG
    AGCCGGGAGA AGGTGGAACT TCTTAGCCGT CTAGACAGAG ACCGGCAGGA ATGGGAGCAG
    CAGAAGAAGG AGCTCTTGTG GAGAATAGAA CAGTTGCAGA AAGAAAACAG TCCCCGGAGA
    AGTGGCAGTT TCCTCTGTGA TCAAAAAGAA GGCACCATCC GCCCCTTTGC CCATCCAGGA
    AGCCCCCGAA TGCCCCGTCC CTTGGGGATG TGGTCCTGTG CAGACACCGA CTCCACCCCA
    TTCGAAGACC GGCCGCTGTC CAAGCTGAAG GAATCAGACA GATGTTCAGC ACGGGAAAAC
    CTCTACTTGG ACGCCTTGTC TCTAGATGAC GAGCCAGAGG AGCCTCCAGC CCGAGAGCCC
    CAGCGGGAGT TCAGGAACTG CCTCCCCCAG GAAGAAGAAA ATCACAAGGG AAATCTTCAA
    AGGGCCGTGT CTGTGTCCTC CATGTCTGAG TTCCAGCGTC TGATGGACGT TTCTCCCTTC
    CTGCCGGAGA AGGGCGTCCC CGCCACCAGC AGCAAGGAGG ACATCACTCC GCCCCTGTCT
    CCTGATGATC TGAAGTACAT CGAGGAGTTC AACAGCAAGA ACTGGGACGA GAGGCCCCCG
    GAGCCCTGGG CAGACAGGAC CGAGGGGGGG CGGGCAGGGA GCGAGGCTGG TGCAGAGCCT
    TTCCCTGACT CCTCCTGGTA CCTCACCACG AGTGTCACCA TGACTACGGA CACCATGACC
    AGCCCAGAGC ACTGCCAGAA GCAGCCCCTT CGGAGCCCCA TGTGCGCGGA GCAGGCCGGG
    GTGCGGGTGC TGCACAGCCC GCCCGCGGTC CGTAGGATGG ATGGCATCGT GGTGGCGGGC
    AGTGAGGGCA AGGGCCGGGC CGAGCCCGAG TGCCCGTTTC CAACTAGCAG AGCCAGGGGG
    GGCACTGGAG ACACAAAAGG GGGTCCCTCA GAACACGTGC TCAGCAGGTG GCCTTGCGCC
    CCCTCCAGGC ACCCCCGAGA CTATGTGGAG GGAGGGCTGC GCCCCCTCGA AAGTGCCCTC
    TGCGCCCCCC TGGGTTTTGC CTCCTCGCTG CACGGCCTGC AGATGTCCAA GAACATGAGT
    GATGACATGA AGGAGGTGGC CTTCTCGGTC AGGAACGCCA TCTGCTCTGG CCCCCCCAAG
    CCTCCGCTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGACGGG AACACAGACC
    GTCCAGACCA TCACCATTGG CCTGCAGACC GAAGCCCTGC GTGGCAGCGC CGTCACCAGC
    AGCCCCCACA AGTGTCTCAC ACCCAAGGCC GGGGGCGGGG CCACGCCCGC GTCATCTCCT
    TCCCGTGGCC TTCGGAGCCG GCAGATGGCC CCCGCCATCG AGAAGGTGCA GGCCAAGTTC
    GAACGCACGT GCTGCTCCCC CAAGTACGGG TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC
    AAAGCTGAGC CGCCCAACAG CAGGACCTCC CTGGGGCTGG CCCCAAAGGG GTGCAGCGAG
    TCCGCCTGGG CCCGTTCCAC CACCACGAGG GAGAGCCCGG TGCACACCGC CATCAACGAT
    GGCCTCTCCA GCCTCTTCAA CATCATCGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG
    AAGGGGACTC GGGCCGGGAG CCGATCTCGC TCAGCAGAAC CCCGGCCCGA GCTGGGCCCA
    GGCCAGGAAA CGGGCCCCAG TTCCCGGGGC CGGTCCCCCA GCCCCATCGG GGCTGGCTCA
    GAGACTTGGA GGGAGGAAGG GGGAGAGGGC ACGCCCTTGA GGCAGGACTT ATCCGCTCCC
    CCTGGTTACA CGTTCACTGA GAACGTAGCC CGGATCCTCA ACAAGAAGCT GTTGGAGCAT
    GCCTTAAAGG AGGAGAGGAG GCAGGCCCCC CACGGGCCCC CAAGTCTTAA CAGTGACAGC
    CACATGGGAG AAACCATCAA AGCTGAACCA GGGTCCATCG AGAACCAAAC TGTCTTACTA
    ACTGCCCCCT GGGAACTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe289884
    Clone ID Related Accession (Same CDS sequence) XM_022421625.1
    Accession Version XM_022421625.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4881bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product microtubule cross-linking factor 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    CAGAGTGGTG GAACCGAGCG CCTTGGAAAC TGCAGGTCTA CAACCAAAAC TCAAAGGAAA
    CTGGCACCCC GGCGCAAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA
    GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG
    GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG
    GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG
    GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA
    GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG
    GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG
    CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT
    GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT
    CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT
    CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG
    AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC
    CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG
    AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC
    GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA
    TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC
    CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG
    GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG
    CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG
    GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG
    GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC
    CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC
    GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGTCCA AACTGAGAGG TCAGCTGGAG
    TGGCAGCCGG GGCAGGAGTG CGGGGACGAG CCAGAGCAGC TGCACCGCCG CGCTGATGGG
    GACGTCGGGA GCTGCCGGCA GGGAGGCCAG AGCTGCTTCA GTCTAGAGAG TGTATCGCTA
    GAGGAGCTGC AGGGTCAGCT TGCGCAGGCG ACCAGACTGC ATCAAGAGGA GACAGAGAAA
    TTTACAAACA AGATTCGGAA GATGGAGGAG GAGCACCTGT ACGCCTTGAG GTGGAAAGAA
    CTGGAAATGC ACAGCCTGGC TTTGCAGAAC ACCCTCCATG AGCAAACCTG GAGTGATGAG
    AGGAACCTGA TGCAGCAGGA GCTCCGGTCT ATGAAGCAGA ACATTTTCCT TTTCTACGTC
    AAACTCAGAT GGCTGCTGAG GCACTGGCGA CAAGGGAAGC AGATGGAGGA AGAAGGCGAG
    GATTTCACTG AGAGCGAGCA TCCAGAGACC CTCCCCAGGC TTGGTGAGCT TGGAGTGCAG
    GGGGACCCGA AGGGGGACGG CCCAGACCAA GATGACAACG GTCCGGGCTG CGGCTTTCCG
    CTGGGAGAGC ATCCTCCACA CTCCCCTGTG CAGATTGGTG ACCATGGATC GAGACTACAG
    CCTACACATG GGAGACAGCT CCACAGGCAG GTGGTGGAAA ACCAGCAGCT GTTCAGTGCA
    CTCAAGAGCC TGCTGGAGGA CCTGCGCGCC GAGCTGCGGG AGGACGAGCA TGCACGGCGG
    CGGCTGCAGC AGCAGTACGC CAGCGACAAA GCCGCCTGGG ACGTGGAGTG GGCTGCACTT
    ACCTGCCGCC TGGAACAGCT GGAAGAGAAG ACTGAGAAGA ATTTGGGAGA AGCATGCTCT
    TCCACCGACA GCAAAGGGGC ATTCAAGAAG GAAAGAGAGA TGCATCAGAA GCTCCTCACT
    GACAGTCATG GCCTGGTCAT GGACCTGCGC TGGCAGATCC ATCACAGTGA GAAGAACTGG
    AGCCGGGAGA AGGTGGAACT TCTTAGCCGT CTAGACAGAG ACCGGCAGGA ATGGGAGCAG
    CAGAAGAAGG AGCTCTTGTG GAGAATAGAA CAGTTGCAGA AAGAAAACAG TCCCCGGAGA
    AGTGGCAGTT TCCTCTGTGA TCAAAAAGAA GGCACCATCC GCCCCTTTGC CCATCCAGGA
    AGCCCCCGAA TGCCCCGTCC CTTGGGGATG TGGTCCTGTG CAGACACCGA CTCCACCCCA
    TTCGAAGACC GGCCGCTGTC CAAGCTGAAG GAATCAGACA GATGTTCAGC ACGGGAAAAC
    CTCTACTTGG ACGCCTTGTC TCTAGATGAC GAGCCAGAGG AGCCTCCAGC CCGAGAGCCC
    CAGCGGGAGT TCAGGAACTG CCTCCCCCAG GAAGAAGAAA ATCACAAGGG AAATCTTCAA
    AGGGCCGTGT CTGTGTCCTC CATGTCTGAG TTCCAGCGTC TGATGGACGT TTCTCCCTTC
    CTGCCGGAGA AGGGCGTCCC CGCCACCAGC AGCAAGGAGG ACATCACTCC GCCCCTGTCT
    CCTGATGATC TGAAGTACAT CGAGGAGTTC AACAGCAAGA ACTGGGACGA GAGGCCCCCG
    GAGCCCTGGG CAGACAGGAC CGAGGGGGGG CGGGCAGGGA GCGAGGCTGG TGCAGAGCCT
    TTCCCTGACT CCTCCTGGTA CCTCACCACG AGTGTCACCA TGACTACGGA CACCATGACC
    AGCCCAGAGC ACTGCCAGAA GCAGCCCCTT CGGAGCCCCA TGTGCGCGGA GCAGGCCGGG
    GTGCGGGTGC TGCACAGCCC GCCCGCGGTC CGTAGGATGG ATGGCATCGT GGTGGCGGGC
    AGTGAGGGCA AGGGCCGGGC CGAGCCCGAG TGCCCGTTTC CAACTAGCAG AGCCAGGGGG
    GGCACTGGAG ACACAAAAGG GGGTCCCTCA GAACACGTGC TCAGCAGGTG GCCTTGCGCC
    CCCTCCAGGC ACCCCCGAGA CTATGTGGAG GGAGGGCTGC GCCCCCTCGA AAGTGCCCTC
    TGCGCCCCCC TGGGTTTTGC CTCCTCGCTG CACGGCCTGC AGATGTCCAA GAACATGAGT
    GATGACATGA AGGAGGTGGC CTTCTCGGTC AGGAACGCCA TCTGCTCTGG CCCCCCCAAG
    CCTCCGCTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGACGGG AACACAGACC
    GTCCAGACCA TCACCATTGG CCTGCAGACC GAAGCCCTGC GTGGCAGCGC CGTCACCAGC
    AGCCCCCACA AGTGTCTCAC ACCCAAGGCC GGGGGCGGGG CCACGCCCGC GTCATCTCCT
    TCCCGTGGCC TTCGGAGCCG GCAGATGGCC CCCGCCATCG AGAAGGTGCA GGCCAAGTTC
    GAACGCACGT GCTGCTCCCC CAAGTACGGG TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC
    AAAGCTGAGC CGCCCAACAG CAGGACCTCC CTGGGGCTGG CCCCAAAGGG GTGCAGCGAG
    TCCGCCTGGG CCCGTTCCAC CACCACGAGG GAGAGCCCGG TGCACACCGC CATCAACGAT
    GGCCTCTCCA GCCTCTTCAA CATCATCGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG
    AAGGGGACTC GGGCCGGGAG CCGATCTCGC TCAGCAGAAC CCCGGCCCGA GCTGGGCCCA
    GGCCAGGAAA CGGGCCCCAG TTCCCGGGGC CGGTCCCCCA GCCCCATCGG GGCTGGCTCA
    GAGACTTGGA GGGAGGAAGG GGGAGAGGGC ACGCCCTTGA GGCAGGACTT ATCCGCTCCC
    CCTGGTTACA CGTTCACTGA GAACGTAGCC CGGATCCTCA ACAAGAAGCT GTTGGAGCAT
    GCCTTAAAGG AGGAGAGGAG GCAGGCCCCC CACGGGCCCC CAAGTCTTAA CAGTGACAGC
    CACATGGGAG AAACCATCAA AGCTGAACCA GGGTCCATCG AGAACCAAAC TGTCTTACTA
    ACTGCCCCCT GGGAACTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022277333.1
    CDS119..4999
    Translation

    Target ORF information:

    RefSeq Version XM_022421625.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022421625.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    CAGAGTGGTG GAACCGAGCG CCTTGGAAAC TGCAGGTCTA CAACCAAAAC TCAAAGGAAA
    CTGGCACCCC GGCGCAAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA
    GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG
    GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG
    GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG
    GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA
    GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG
    GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG
    CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT
    GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT
    CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT
    CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG
    AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC
    CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG
    AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC
    GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA
    TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC
    CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG
    GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG
    CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG
    GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG
    GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC
    CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC
    GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGTCCA AACTGAGAGG TCAGCTGGAG
    TGGCAGCCGG GGCAGGAGTG CGGGGACGAG CCAGAGCAGC TGCACCGCCG CGCTGATGGG
    GACGTCGGGA GCTGCCGGCA GGGAGGCCAG AGCTGCTTCA GTCTAGAGAG TGTATCGCTA
    GAGGAGCTGC AGGGTCAGCT TGCGCAGGCG ACCAGACTGC ATCAAGAGGA GACAGAGAAA
    TTTACAAACA AGATTCGGAA GATGGAGGAG GAGCACCTGT ACGCCTTGAG GTGGAAAGAA
    CTGGAAATGC ACAGCCTGGC TTTGCAGAAC ACCCTCCATG AGCAAACCTG GAGTGATGAG
    AGGAACCTGA TGCAGCAGGA GCTCCGGTCT ATGAAGCAGA ACATTTTCCT TTTCTACGTC
    AAACTCAGAT GGCTGCTGAG GCACTGGCGA CAAGGGAAGC AGATGGAGGA AGAAGGCGAG
    GATTTCACTG AGAGCGAGCA TCCAGAGACC CTCCCCAGGC TTGGTGAGCT TGGAGTGCAG
    GGGGACCCGA AGGGGGACGG CCCAGACCAA GATGACAACG GTCCGGGCTG CGGCTTTCCG
    CTGGGAGAGC ATCCTCCACA CTCCCCTGTG CAGATTGGTG ACCATGGATC GAGACTACAG
    CCTACACATG GGAGACAGCT CCACAGGCAG GTGGTGGAAA ACCAGCAGCT GTTCAGTGCA
    CTCAAGAGCC TGCTGGAGGA CCTGCGCGCC GAGCTGCGGG AGGACGAGCA TGCACGGCGG
    CGGCTGCAGC AGCAGTACGC CAGCGACAAA GCCGCCTGGG ACGTGGAGTG GGCTGCACTT
    ACCTGCCGCC TGGAACAGCT GGAAGAGAAG ACTGAGAAGA ATTTGGGAGA AGCATGCTCT
    TCCACCGACA GCAAAGGGGC ATTCAAGAAG GAAAGAGAGA TGCATCAGAA GCTCCTCACT
    GACAGTCATG GCCTGGTCAT GGACCTGCGC TGGCAGATCC ATCACAGTGA GAAGAACTGG
    AGCCGGGAGA AGGTGGAACT TCTTAGCCGT CTAGACAGAG ACCGGCAGGA ATGGGAGCAG
    CAGAAGAAGG AGCTCTTGTG GAGAATAGAA CAGTTGCAGA AAGAAAACAG TCCCCGGAGA
    AGTGGCAGTT TCCTCTGTGA TCAAAAAGAA GGCACCATCC GCCCCTTTGC CCATCCAGGA
    AGCCCCCGAA TGCCCCGTCC CTTGGGGATG TGGTCCTGTG CAGACACCGA CTCCACCCCA
    TTCGAAGACC GGCCGCTGTC CAAGCTGAAG GAATCAGACA GATGTTCAGC ACGGGAAAAC
    CTCTACTTGG ACGCCTTGTC TCTAGATGAC GAGCCAGAGG AGCCTCCAGC CCGAGAGCCC
    CAGCGGGAGT TCAGGAACTG CCTCCCCCAG GAAGAAGAAA ATCACAAGGG AAATCTTCAA
    AGGGCCGTGT CTGTGTCCTC CATGTCTGAG TTCCAGCGTC TGATGGACGT TTCTCCCTTC
    CTGCCGGAGA AGGGCGTCCC CGCCACCAGC AGCAAGGAGG ACATCACTCC GCCCCTGTCT
    CCTGATGATC TGAAGTACAT CGAGGAGTTC AACAGCAAGA ACTGGGACGA GAGGCCCCCG
    GAGCCCTGGG CAGACAGGAC CGAGGGGGGG CGGGCAGGGA GCGAGGCTGG TGCAGAGCCT
    TTCCCTGACT CCTCCTGGTA CCTCACCACG AGTGTCACCA TGACTACGGA CACCATGACC
    AGCCCAGAGC ACTGCCAGAA GCAGCCCCTT CGGAGCCCCA TGTGCGCGGA GCAGGCCGGG
    GTGCGGGTGC TGCACAGCCC GCCCGCGGTC CGTAGGATGG ATGGCATCGT GGTGGCGGGC
    AGTGAGGGCA AGGGCCGGGC CGAGCCCGAG TGCCCGTTTC CAACTAGCAG AGCCAGGGGG
    GGCACTGGAG ACACAAAAGG GGGTCCCTCA GAACACGTGC TCAGCAGGTG GCCTTGCGCC
    CCCTCCAGGC ACCCCCGAGA CTATGTGGAG GGAGGGCTGC GCCCCCTCGA AAGTGCCCTC
    TGCGCCCCCC TGGGTTTTGC CTCCTCGCTG CACGGCCTGC AGATGTCCAA GAACATGAGT
    GATGACATGA AGGAGGTGGC CTTCTCGGTC AGGAACGCCA TCTGCTCTGG CCCCCCCAAG
    CCTCCGCTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGACGGG AACACAGACC
    GTCCAGACCA TCACCATTGG CCTGCAGACC GAAGCCCTGC GTGGCAGCGC CGTCACCAGC
    AGCCCCCACA AGTGTCTCAC ACCCAAGGCC GGGGGCGGGG CCACGCCCGC GTCATCTCCT
    TCCCGTGGCC TTCGGAGCCG GCAGATGGCC CCCGCCATCG AGAAGGTGCA GGCCAAGTTC
    GAACGCACGT GCTGCTCCCC CAAGTACGGG TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC
    AAAGCTGAGC CGCCCAACAG CAGGACCTCC CTGGGGCTGG CCCCAAAGGG GTGCAGCGAG
    TCCGCCTGGG CCCGTTCCAC CACCACGAGG GAGAGCCCGG TGCACACCGC CATCAACGAT
    GGCCTCTCCA GCCTCTTCAA CATCATCGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG
    AAGGGGACTC GGGCCGGGAG CCGATCTCGC TCAGCAGAAC CCCGGCCCGA GCTGGGCCCA
    GGCCAGGAAA CGGGCCCCAG TTCCCGGGGC CGGTCCCCCA GCCCCATCGG GGCTGGCTCA
    GAGACTTGGA GGGAGGAAGG GGGAGAGGGC ACGCCCTTGA GGCAGGACTT ATCCGCTCCC
    CCTGGTTACA CGTTCACTGA GAACGTAGCC CGGATCCTCA ACAAGAAGCT GTTGGAGCAT
    GCCTTAAAGG AGGAGAGGAG GCAGGCCCCC CACGGGCCCC CAAGTCTTAA CAGTGACAGC
    CACATGGGAG AAACCATCAA AGCTGAACCA GGGTCCATCG AGAACCAAAC TGTCTTACTA
    ACTGCCCCCT GGGAACTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe289885
    Clone ID Related Accession (Same CDS sequence) XM_022421626.1
    Accession Version XM_022421626.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product microtubule cross-linking factor 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    CAGAGTGGTG GAACCGAGCG CCTTGGAAAC TGCAGGTCTA CAACCAAAAC TCAAAGGAAA
    CTGGCACCCC GGCGCAAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA
    GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG
    GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG
    GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG
    GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA
    GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG
    GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG
    CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT
    GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT
    CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT
    CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG
    AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC
    CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG
    AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC
    GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA
    TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC
    CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG
    GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG
    CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG
    GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG
    GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC
    CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC
    GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGATGG AGGAGGAGCA CCTGTACGCC
    TTGAGGTGGA AAGAACTGGA AATGCACAGC CTGGCTTTGC AGAACACCCT CCATGAGCAA
    ACCTGGAGTG ATGAGAGGAA CCTGATGCAG CAGGAGCTCC GGTCTATGAA GCAGAACATT
    TTCCTTTTCT ACGTCAAACT CAGATGGCTG CTGAGGCACT GGCGACAAGG GAAGCAGATG
    GAGGAAGAAG GCGAGGATTT CACTGAGAGC GAGCATCCAG AGACCCTCCC CAGGCTTGGT
    GAGCTTGGAG TGCAGGGGGA CCCGAAGGGG GACGGCCCAG ACCAAGATGA CAACGGTCCG
    GGCTGCGGCT TTCCGCTGGG AGAGCATCCT CCACACTCCC CTGTGCAGAT TGGTGACCAT
    GGATCGAGAC TACAGCCTAC ACATGGGAGA CAGCTCCACA GGCAGGTGGT GGAAAACCAG
    CAGCTGTTCA GTGCACTCAA GAGCCTGCTG GAGGACCTGC GCGCCGAGCT GCGGGAGGAC
    GAGCATGCAC GGCGGCGGCT GCAGCAGCAG TACGCCAGCG ACAAAGCCGC CTGGGACGTG
    GAGTGGGCTG CACTTACCTG CCGCCTGGAA CAGGTGCCTG CCTGCGGGGT CTGTCCTGGG
    TCTCCCCAGC TCCTCCTCTG CCATCTGGGC CAGCTGGAAG AGAAGACTGA GAAGAATTTG
    GGAGAAGCAT GCTCTTCCAC CGACAGCAAA GGGGCATTCA AGAAGGAAAG AGAGATGCAT
    CAGAAGCTCC TCACTGACAG TCATGGCCTG GTCATGGACC TGCGCTGGCA GATCCATCAC
    AGTGAGAAGA ACTGGAGCCG GGAGAAGGTG GAACTTCTTA GCCGTCTAGA CAGAGACCGG
    CAGGAATGGG AGCAGCAGAA GAAGGAGCTC TTGTGGAGAA TAGAACAGTT GCAGAAAGAA
    AACAGTCCCC GGAGAAGTGG CAGTTTCCTC TGTGATCAAA AAGAAGGCAC CATCCGCCCC
    TTTGCCCATC CAGGAAGCCC CCGAATGCCC CGTCCCTTGG GGATGTGGTC CTGTGCAGAC
    ACCGACTCCA CCCCATTCGA AGACCGGCCG CTGTCCAAGC TGAAGGAATC AGACAGATGT
    TCAGCACGGG AAAACCTCTA CTTGGACGCC TTGTCTCTAG ATGACGAGCC AGAGGAGCCT
    CCAGCCCGAG AGCCCCAGCG GGAGTTCAGG AACTGCCTCC CCCAGGAAGA AGAAAATCAC
    AAGGGAAATC TTCAAAGGGC CGTGTCTGTG TCCTCCATGT CTGAGTTCCA GCGTCTGATG
    GACGTTTCTC CCTTCCTGCC GGAGAAGGGC GTCCCCGCCA CCAGCAGCAA GGAGGACATC
    ACTCCGCCCC TGTCTCCTGA TGATCTGAAG TACATCGAGG AGTTCAACAG CAAGAACTGG
    GACGAGAGGC CCCCGGAGCC CTGGGCAGAC AGGACCGAGG GGGGGCGGGC AGGGAGCGAG
    GCTGGTGCAG AGCCTTTCCC TGACTCCTCC TGGTACCTCA CCACGAGTGT CACCATGACT
    ACGGACACCA TGACCAGCCC AGAGCACTGC CAGAAGCAGC CCCTTCGGAG CCCCATGTGC
    GCGGAGCAGG CCGGGGTGCG GGTGCTGCAC AGCCCGCCCG CGGTCCGTAG GATGGATGGC
    ATCGTGGTGG CGGGCAGTGA GGGCAAGGGC CGGGCCGAGC CCGAGTGCCC GTTTCCAACT
    AGCAGAGCCA GGGGGGGCAC TGGAGACACA AAAGGGGGTC CCTCAGAACA CGTGCTCAGC
    AGGTGGCCTT GCGCCCCCTC CAGGCACCCC CGAGACTATG TGGAGGGAGG GCTGCGCCCC
    CTCGAAAGTG CCCTCTGCGC CCCCCTGGGT TTTGCCTCCT CGCTGCACGG CCTGCAGATG
    TCCAAGAACA TGAGTGATGA CATGAAGGAG GTGGCCTTCT CGGTCAGGAA CGCCATCTGC
    TCTGGCCCCC CCAAGCCTCC GCTCAAGGAC ATGGCCTGCC AGACCAATGG GTCCCGGACG
    ACGGGAACAC AGACCGTCCA GACCATCACC ATTGGCCTGC AGACCGAAGC CCTGCGTGGC
    AGCGCCGTCA CCAGCAGCCC CCACAAGTGT CTCACACCCA AGGCCGGGGG CGGGGCCACG
    CCCGCGTCAT CTCCTTCCCG TGGCCTTCGG AGCCGGCAGA TGGCCCCCGC CATCGAGAAG
    GTGCAGGCCA AGTTCGAACG CACGTGCTGC TCCCCCAAGT ACGGGTCTCC CAAGCTGCAG
    AGGAAGCCCC TCCCCAAAGC TGAGCCGCCC AACAGCAGGA CCTCCCTGGG GCTGGCCCCA
    AAGGGGTGCA GCGAGTCCGC CTGGGCCCGT TCCACCACCA CGAGGGAGAG CCCGGTGCAC
    ACCGCCATCA ACGATGGCCT CTCCAGCCTC TTCAACATCA TCGACCACAG CCCCGTGGTG
    CAGGACCCCT TCCAGAAGGG GACTCGGGCC GGGAGCCGAT CTCGCTCAGC AGAACCCCGG
    CCCGAGCTGG GCCCAGGCCA GGAAACGGGC CCCAGTTCCC GGGGCCGGTC CCCCAGCCCC
    ATCGGGGCTG GCTCAGAGAC TTGGAGGGAG GAAGGGGGAG AGGGCACGCC CTTGAGGCAG
    GACTTATCCG CTCCCCCTGG TTACACGTTC ACTGAGAACG TAGCCCGGAT CCTCAACAAG
    AAGCTGTTGG AGCATGCCTT AAAGGAGGAG AGGAGGCAGG CCCCCCACGG GCCCCCAAGT
    CTTAACAGTG ACAGCCACAT GGGAGAAACC ATCAAAGCTG AACCAGGGTC CATCGAGGAA
    CTACCTTGTT CCGCACTAGC TCCATCCCTA GAACCCTGCT TCTCCAGGCC CGAGAGACCA
    GCAAACCGTC GCCTTCCGTC CCGTTGGGCC TCACATTCCC CCACTGCCTC ACTGGCTCAG
    TCACCCGGAG ACCCAGCCTC CTTGGAGGAG CTCGGCGATG AGGAGCCACC GGGGGAGAAG
    ACACACCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022277334.1
    CDS117..4988
    Translation

    Target ORF information:

    RefSeq Version XM_022421626.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022421626.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    CAGAGTGGTG GAACCGAGCG CCTTGGAAAC TGCAGGTCTA CAACCAAAAC TCAAAGGAAA
    CTGGCACCCC GGCGCAAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA
    GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG
    GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG
    GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG
    GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA
    GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG
    GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG
    CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT
    GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT
    CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT
    CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG
    AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC
    CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG
    AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC
    GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA
    TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC
    CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG
    GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG
    CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG
    GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG
    GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC
    CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC
    GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGATGG AGGAGGAGCA CCTGTACGCC
    TTGAGGTGGA AAGAACTGGA AATGCACAGC CTGGCTTTGC AGAACACCCT CCATGAGCAA
    ACCTGGAGTG ATGAGAGGAA CCTGATGCAG CAGGAGCTCC GGTCTATGAA GCAGAACATT
    TTCCTTTTCT ACGTCAAACT CAGATGGCTG CTGAGGCACT GGCGACAAGG GAAGCAGATG
    GAGGAAGAAG GCGAGGATTT CACTGAGAGC GAGCATCCAG AGACCCTCCC CAGGCTTGGT
    GAGCTTGGAG TGCAGGGGGA CCCGAAGGGG GACGGCCCAG ACCAAGATGA CAACGGTCCG
    GGCTGCGGCT TTCCGCTGGG AGAGCATCCT CCACACTCCC CTGTGCAGAT TGGTGACCAT
    GGATCGAGAC TACAGCCTAC ACATGGGAGA CAGCTCCACA GGCAGGTGGT GGAAAACCAG
    CAGCTGTTCA GTGCACTCAA GAGCCTGCTG GAGGACCTGC GCGCCGAGCT GCGGGAGGAC
    GAGCATGCAC GGCGGCGGCT GCAGCAGCAG TACGCCAGCG ACAAAGCCGC CTGGGACGTG
    GAGTGGGCTG CACTTACCTG CCGCCTGGAA CAGGTGCCTG CCTGCGGGGT CTGTCCTGGG
    TCTCCCCAGC TCCTCCTCTG CCATCTGGGC CAGCTGGAAG AGAAGACTGA GAAGAATTTG
    GGAGAAGCAT GCTCTTCCAC CGACAGCAAA GGGGCATTCA AGAAGGAAAG AGAGATGCAT
    CAGAAGCTCC TCACTGACAG TCATGGCCTG GTCATGGACC TGCGCTGGCA GATCCATCAC
    AGTGAGAAGA ACTGGAGCCG GGAGAAGGTG GAACTTCTTA GCCGTCTAGA CAGAGACCGG
    CAGGAATGGG AGCAGCAGAA GAAGGAGCTC TTGTGGAGAA TAGAACAGTT GCAGAAAGAA
    AACAGTCCCC GGAGAAGTGG CAGTTTCCTC TGTGATCAAA AAGAAGGCAC CATCCGCCCC
    TTTGCCCATC CAGGAAGCCC CCGAATGCCC CGTCCCTTGG GGATGTGGTC CTGTGCAGAC
    ACCGACTCCA CCCCATTCGA AGACCGGCCG CTGTCCAAGC TGAAGGAATC AGACAGATGT
    TCAGCACGGG AAAACCTCTA CTTGGACGCC TTGTCTCTAG ATGACGAGCC AGAGGAGCCT
    CCAGCCCGAG AGCCCCAGCG GGAGTTCAGG AACTGCCTCC CCCAGGAAGA AGAAAATCAC
    AAGGGAAATC TTCAAAGGGC CGTGTCTGTG TCCTCCATGT CTGAGTTCCA GCGTCTGATG
    GACGTTTCTC CCTTCCTGCC GGAGAAGGGC GTCCCCGCCA CCAGCAGCAA GGAGGACATC
    ACTCCGCCCC TGTCTCCTGA TGATCTGAAG TACATCGAGG AGTTCAACAG CAAGAACTGG
    GACGAGAGGC CCCCGGAGCC CTGGGCAGAC AGGACCGAGG GGGGGCGGGC AGGGAGCGAG
    GCTGGTGCAG AGCCTTTCCC TGACTCCTCC TGGTACCTCA CCACGAGTGT CACCATGACT
    ACGGACACCA TGACCAGCCC AGAGCACTGC CAGAAGCAGC CCCTTCGGAG CCCCATGTGC
    GCGGAGCAGG CCGGGGTGCG GGTGCTGCAC AGCCCGCCCG CGGTCCGTAG GATGGATGGC
    ATCGTGGTGG CGGGCAGTGA GGGCAAGGGC CGGGCCGAGC CCGAGTGCCC GTTTCCAACT
    AGCAGAGCCA GGGGGGGCAC TGGAGACACA AAAGGGGGTC CCTCAGAACA CGTGCTCAGC
    AGGTGGCCTT GCGCCCCCTC CAGGCACCCC CGAGACTATG TGGAGGGAGG GCTGCGCCCC
    CTCGAAAGTG CCCTCTGCGC CCCCCTGGGT TTTGCCTCCT CGCTGCACGG CCTGCAGATG
    TCCAAGAACA TGAGTGATGA CATGAAGGAG GTGGCCTTCT CGGTCAGGAA CGCCATCTGC
    TCTGGCCCCC CCAAGCCTCC GCTCAAGGAC ATGGCCTGCC AGACCAATGG GTCCCGGACG
    ACGGGAACAC AGACCGTCCA GACCATCACC ATTGGCCTGC AGACCGAAGC CCTGCGTGGC
    AGCGCCGTCA CCAGCAGCCC CCACAAGTGT CTCACACCCA AGGCCGGGGG CGGGGCCACG
    CCCGCGTCAT CTCCTTCCCG TGGCCTTCGG AGCCGGCAGA TGGCCCCCGC CATCGAGAAG
    GTGCAGGCCA AGTTCGAACG CACGTGCTGC TCCCCCAAGT ACGGGTCTCC CAAGCTGCAG
    AGGAAGCCCC TCCCCAAAGC TGAGCCGCCC AACAGCAGGA CCTCCCTGGG GCTGGCCCCA
    AAGGGGTGCA GCGAGTCCGC CTGGGCCCGT TCCACCACCA CGAGGGAGAG CCCGGTGCAC
    ACCGCCATCA ACGATGGCCT CTCCAGCCTC TTCAACATCA TCGACCACAG CCCCGTGGTG
    CAGGACCCCT TCCAGAAGGG GACTCGGGCC GGGAGCCGAT CTCGCTCAGC AGAACCCCGG
    CCCGAGCTGG GCCCAGGCCA GGAAACGGGC CCCAGTTCCC GGGGCCGGTC CCCCAGCCCC
    ATCGGGGCTG GCTCAGAGAC TTGGAGGGAG GAAGGGGGAG AGGGCACGCC CTTGAGGCAG
    GACTTATCCG CTCCCCCTGG TTACACGTTC ACTGAGAACG TAGCCCGGAT CCTCAACAAG
    AAGCTGTTGG AGCATGCCTT AAAGGAGGAG AGGAGGCAGG CCCCCCACGG GCCCCCAAGT
    CTTAACAGTG ACAGCCACAT GGGAGAAACC ATCAAAGCTG AACCAGGGTC CATCGAGGAA
    CTACCTTGTT CCGCACTAGC TCCATCCCTA GAACCCTGCT TCTCCAGGCC CGAGAGACCA
    GCAAACCGTC GCCTTCCGTC CCGTTGGGCC TCACATTCCC CCACTGCCTC ACTGGCTCAG
    TCACCCGGAG ACCCAGCCTC CTTGGAGGAG CTCGGCGATG AGGAGCCACC GGGGGAGAAG
    ACACACCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe289886
    Clone ID Related Accession (Same CDS sequence) XM_022421627.1
    Accession Version XM_022421627.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5097bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product microtubule cross-linking factor 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    CAGAGTGGTG GAACCGAGCG CCTTGGAAAC TGCAGGTCTA CAACCAAAAC TCAAAGGAAA
    CTGGCACCCC GGCGCAAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA
    GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG
    GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG
    GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG
    GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA
    GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG
    GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG
    CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT
    GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT
    CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT
    CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG
    AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC
    CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG
    AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC
    GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA
    TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC
    CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG
    GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG
    CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG
    GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG
    GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC
    CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC
    GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGTCCA AACTGAGAGG TCAGCTGGAG
    TGGCAGCCGG GGCAGGAGTG CGGGGACGAG CCAGAGCAGC TGCACCGCCG CGCTGATGGG
    GACGTCGGGA GCTGCCGGCA GGGAGGCCAG AGCTGCTTCA GTCTAGAGAG TGTATCGCTA
    GAGGAGCTGC AGGGTCAGCT TGCGCAGGCG ACCAGACTGC ATCAAGAGGA GACAGAGAAA
    TTTACAAACA AGATTCGGAA GATGGAGGAG GAGCACCTGT ACGCCTTGAG GTGGAAAGAA
    CTGGAAATGC ACAGCCTGGC TTTGCAGAAC ACCCTCCATG AGCAAACCTG GAGTGATGAG
    AGGAACCTGA TGCAGCAGGA GCTCCGGTCT ATGAAGCAGA ACATTTTCCT TTTCTACGTC
    AAACTCAGAT GGCTGCTGAG GCACTGGCGA CAAGGGAAGC AGATGGAGGA AGAAGGCGAG
    GATTTCACTG AGAGCGAGCA TCCAGAGACC CTCCCCAGGC TTGGTGAGCT TGGAGTGCAG
    GGGGACCCGA AGGGGGACGG CCCAGACCAA GATGACAACG GTCCGGGCTG CGGCTTTCCG
    CTGGGAGAGC ATCCTCCACA CTCCCCTGTG CAGATTGGTG ACCATGGATC GAGACTACAG
    CCTACACATG GGAGACAGCT CCACAGGCAG GTGGTGGAAA ACCAGCAGCT GTTCAGTGCA
    CTCAAGAGCC TGCTGGAGGA CCTGCGCGCC GAGCTGCGGG AGGACGAGCA TGCACGGCGG
    CGGCTGCAGC AGCAGTACGC CAGCGACAAA GCCGCCTGGG ACGTGGAGTG GGCTGCACTT
    ACCTGCCGCC TGGAACAGGT GCCTGCCTGC GGGGTCTGTC CTGGGTCTCC CCAGCTCCTC
    CTCTGCCATC TGGGCCAGCT GGAAGAGAAG ACTGAGAAGA ATTTGGGAGA AGCATGCTCT
    TCCACCGACA GCAAAGGGGC ATTCAAGAAG GAAAGAGAGA TGCATCAGAA GCTCCTCACT
    GACAGTCATG GCCTGGTCAT GGACCTGCGC TGGCAGATCC ATCACAGTGA GAAGAACTGG
    AGCCGGGAGA AGGTGGAACT TCTTAGCCGT CTAGACAGAG ACCGGCAGGA ATGGGAGCAG
    CAGAAGAAGG AGCTCTTGTG GAGAATAGAA CAGTTGCAGA AAGAAAACAG TCCCCGGAGA
    AGTGGCAGTT TCCTCTGTGA TCAAAAAGAA GGCACCATCC GCCCCTTTGC CCATCCAGGA
    AGCCCCCGAA TGCCCCGTCC CTTGGGGATG TGGTCCTGTG CAGACACCGA CTCCACCCCA
    TTCGAAGACC GGCCGCTGTC CAAGCTGAAG GAATCAGACA GATGTTCAGC ACGGGAAAAC
    CTCTACTTGG ACGCCTTGTC TCTAGATGAC GAGCCAGAGG AGCCTCCAGC CCGAGAGCCC
    CAGCGGGAGT TCAGGAACTG CCTCCCCCAG GAAGAAGAAA ATCACAAGGG AAATCTTCAA
    AGGGCCGTGT CTGTGTCCTC CATGTCTGAG TTCCAGCGTC TGATGGACGT TTCTCCCTTC
    CTGCCGGAGA AGGGCGTCCC CGCCACCAGC AGCAAGGAGG ACATCACTCC GCCCCTGTCT
    CCTGATGATC TGAAGTACAT CGAGGAGTTC AACAGCAAGA ACTGGGACGA GAGGCCCCCG
    GAGCCCTGGG CAGACAGGAC CGAGGGGGGG CGGGCAGGGA GCGAGGCTGG TGCAGAGCCT
    TTCCCTGACT CCTCCTGGTA CCTCACCACG AGTGTCACCA TGACTACGGA CACCATGACC
    AGCCCAGAGC ACTGCCAGAA GCAGCCCCTT CGGAGCCCCA TGTGCGCGGA GCAGGCCGGG
    GTGCGGGTGC TGCACAGCCC GCCCGCGGTC CGTAGGATGG ATGGCATCGT GGTGGCGGGC
    AGTGAGGGCA AGGGCCGGGC CGAGCCCGAG TGCCCGTTTC CAACTAGCAG AGCCAGGGGG
    GGCACTGGAG ACACAAAAGG GGGTCCCTCA GAACACGTGC TCAGCAGGTG GCCTTGCGCC
    CCCTCCAGGC ACCCCCGAGA CTATGTGGAG GGAGGGCTGC GCCCCCTCGA AAGTGCCCTC
    TGCGCCCCCC TGGGTTTTGC CTCCTCGCTG CACGGCCTGC AGATGTCCAA GAACATGAGT
    GATGACATGA AGGAGGTGGC CTTCTCGGTC AGGAACGCCA TCTGCTCTGG CCCCCCCAAG
    CCTCCGCTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGACGGG AACACAGACC
    GTCCAGACCA TCACCATTGG CCTGCAGACC GAAGCCCTGC GTGGCAGCGC CGTCACCAGC
    AGCCCCCACA AGTGTCTCAC ACCCAAGGCC GGGGGCGGGG CCACGCCCGC GTCATCTCCT
    TCCCGTGGCC TTCGGAGCCG GCAGATGGCC CCCGCCATCG AGAAGGTGCA GGCCAAGTTC
    GAACGCACGT GCTGCTCCCC CAAGTACGGG TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC
    AAAGCTGAGC CGCCCAACAG CAGGACCTCC CTGGGGCTGG CCCCAAAGGG GTGCAGCGAG
    TCCGCCTGGG CCCGTTCCAC CACCACGAGG GAGAGCCCGG TGCACACCGC CATCAACGAT
    GGCCTCTCCA GCCTCTTCAA CATCATCGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG
    AAGGGGACTC GGGCCGGGAG CCGATCTCGC TCAGCAGAAC CCCGGCCCGA GCTGGGCCCA
    GGCCAGGAAA CGGGCCCCAG TTCCCGGGGC CGGTCCCCCA GCCCCATCGG GGCTGGCTCA
    GAGACTTGGA GGGAGGAAGG GGGAGAGGGC ACGCCCTTGA GGCAGGACTT ATCCGCTCCC
    CCTGGTTACA CGTTCACTGA GAACGTAGCC CGGATCCTCA ACAAGAAGCT GTTGGAGCAT
    GCCTTAAAGG AGGAGAGGAG GCAGGCCCCC CACGGGCCCC CAAGTCTTAA CAGTGACAGC
    CACATGGGAG AAACCATCAA AGCTGAACCA GGGTCCATCG AGGAACTACC TTGTTCCGCA
    CTAGCTCCAT CCCTAGAACC CTGCTTCTCC AGGCCCGAGA GACCAGCAAA CCGTCGCCTT
    CCGTCCCGTT GGGCCTCACA TTCCCCCACT GCCTCACTGG CTCAGTCACC CGGAGACCCA
    GCCTCCTTGG AGGAGCTCGG CGATGAGGAG CCACCGGGGG AGAAGACACA CCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022277335.1
    CDS132..5228
    Translation

    Target ORF information:

    RefSeq Version XM_022421627.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022421627.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    CAGAGTGGTG GAACCGAGCG CCTTGGAAAC TGCAGGTCTA CAACCAAAAC TCAAAGGAAA
    CTGGCACCCC GGCGCAAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA
    GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG
    GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG
    GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG
    GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA
    GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG
    GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG
    CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT
    GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT
    CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT
    CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG
    AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC
    CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG
    AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC
    GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA
    TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC
    CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG
    GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG
    CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG
    GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG
    GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC
    CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC
    GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGTCCA AACTGAGAGG TCAGCTGGAG
    TGGCAGCCGG GGCAGGAGTG CGGGGACGAG CCAGAGCAGC TGCACCGCCG CGCTGATGGG
    GACGTCGGGA GCTGCCGGCA GGGAGGCCAG AGCTGCTTCA GTCTAGAGAG TGTATCGCTA
    GAGGAGCTGC AGGGTCAGCT TGCGCAGGCG ACCAGACTGC ATCAAGAGGA GACAGAGAAA
    TTTACAAACA AGATTCGGAA GATGGAGGAG GAGCACCTGT ACGCCTTGAG GTGGAAAGAA
    CTGGAAATGC ACAGCCTGGC TTTGCAGAAC ACCCTCCATG AGCAAACCTG GAGTGATGAG
    AGGAACCTGA TGCAGCAGGA GCTCCGGTCT ATGAAGCAGA ACATTTTCCT TTTCTACGTC
    AAACTCAGAT GGCTGCTGAG GCACTGGCGA CAAGGGAAGC AGATGGAGGA AGAAGGCGAG
    GATTTCACTG AGAGCGAGCA TCCAGAGACC CTCCCCAGGC TTGGTGAGCT TGGAGTGCAG
    GGGGACCCGA AGGGGGACGG CCCAGACCAA GATGACAACG GTCCGGGCTG CGGCTTTCCG
    CTGGGAGAGC ATCCTCCACA CTCCCCTGTG CAGATTGGTG ACCATGGATC GAGACTACAG
    CCTACACATG GGAGACAGCT CCACAGGCAG GTGGTGGAAA ACCAGCAGCT GTTCAGTGCA
    CTCAAGAGCC TGCTGGAGGA CCTGCGCGCC GAGCTGCGGG AGGACGAGCA TGCACGGCGG
    CGGCTGCAGC AGCAGTACGC CAGCGACAAA GCCGCCTGGG ACGTGGAGTG GGCTGCACTT
    ACCTGCCGCC TGGAACAGGT GCCTGCCTGC GGGGTCTGTC CTGGGTCTCC CCAGCTCCTC
    CTCTGCCATC TGGGCCAGCT GGAAGAGAAG ACTGAGAAGA ATTTGGGAGA AGCATGCTCT
    TCCACCGACA GCAAAGGGGC ATTCAAGAAG GAAAGAGAGA TGCATCAGAA GCTCCTCACT
    GACAGTCATG GCCTGGTCAT GGACCTGCGC TGGCAGATCC ATCACAGTGA GAAGAACTGG
    AGCCGGGAGA AGGTGGAACT TCTTAGCCGT CTAGACAGAG ACCGGCAGGA ATGGGAGCAG
    CAGAAGAAGG AGCTCTTGTG GAGAATAGAA CAGTTGCAGA AAGAAAACAG TCCCCGGAGA
    AGTGGCAGTT TCCTCTGTGA TCAAAAAGAA GGCACCATCC GCCCCTTTGC CCATCCAGGA
    AGCCCCCGAA TGCCCCGTCC CTTGGGGATG TGGTCCTGTG CAGACACCGA CTCCACCCCA
    TTCGAAGACC GGCCGCTGTC CAAGCTGAAG GAATCAGACA GATGTTCAGC ACGGGAAAAC
    CTCTACTTGG ACGCCTTGTC TCTAGATGAC GAGCCAGAGG AGCCTCCAGC CCGAGAGCCC
    CAGCGGGAGT TCAGGAACTG CCTCCCCCAG GAAGAAGAAA ATCACAAGGG AAATCTTCAA
    AGGGCCGTGT CTGTGTCCTC CATGTCTGAG TTCCAGCGTC TGATGGACGT TTCTCCCTTC
    CTGCCGGAGA AGGGCGTCCC CGCCACCAGC AGCAAGGAGG ACATCACTCC GCCCCTGTCT
    CCTGATGATC TGAAGTACAT CGAGGAGTTC AACAGCAAGA ACTGGGACGA GAGGCCCCCG
    GAGCCCTGGG CAGACAGGAC CGAGGGGGGG CGGGCAGGGA GCGAGGCTGG TGCAGAGCCT
    TTCCCTGACT CCTCCTGGTA CCTCACCACG AGTGTCACCA TGACTACGGA CACCATGACC
    AGCCCAGAGC ACTGCCAGAA GCAGCCCCTT CGGAGCCCCA TGTGCGCGGA GCAGGCCGGG
    GTGCGGGTGC TGCACAGCCC GCCCGCGGTC CGTAGGATGG ATGGCATCGT GGTGGCGGGC
    AGTGAGGGCA AGGGCCGGGC CGAGCCCGAG TGCCCGTTTC CAACTAGCAG AGCCAGGGGG
    GGCACTGGAG ACACAAAAGG GGGTCCCTCA GAACACGTGC TCAGCAGGTG GCCTTGCGCC
    CCCTCCAGGC ACCCCCGAGA CTATGTGGAG GGAGGGCTGC GCCCCCTCGA AAGTGCCCTC
    TGCGCCCCCC TGGGTTTTGC CTCCTCGCTG CACGGCCTGC AGATGTCCAA GAACATGAGT
    GATGACATGA AGGAGGTGGC CTTCTCGGTC AGGAACGCCA TCTGCTCTGG CCCCCCCAAG
    CCTCCGCTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGACGGG AACACAGACC
    GTCCAGACCA TCACCATTGG CCTGCAGACC GAAGCCCTGC GTGGCAGCGC CGTCACCAGC
    AGCCCCCACA AGTGTCTCAC ACCCAAGGCC GGGGGCGGGG CCACGCCCGC GTCATCTCCT
    TCCCGTGGCC TTCGGAGCCG GCAGATGGCC CCCGCCATCG AGAAGGTGCA GGCCAAGTTC
    GAACGCACGT GCTGCTCCCC CAAGTACGGG TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC
    AAAGCTGAGC CGCCCAACAG CAGGACCTCC CTGGGGCTGG CCCCAAAGGG GTGCAGCGAG
    TCCGCCTGGG CCCGTTCCAC CACCACGAGG GAGAGCCCGG TGCACACCGC CATCAACGAT
    GGCCTCTCCA GCCTCTTCAA CATCATCGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG
    AAGGGGACTC GGGCCGGGAG CCGATCTCGC TCAGCAGAAC CCCGGCCCGA GCTGGGCCCA
    GGCCAGGAAA CGGGCCCCAG TTCCCGGGGC CGGTCCCCCA GCCCCATCGG GGCTGGCTCA
    GAGACTTGGA GGGAGGAAGG GGGAGAGGGC ACGCCCTTGA GGCAGGACTT ATCCGCTCCC
    CCTGGTTACA CGTTCACTGA GAACGTAGCC CGGATCCTCA ACAAGAAGCT GTTGGAGCAT
    GCCTTAAAGG AGGAGAGGAG GCAGGCCCCC CACGGGCCCC CAAGTCTTAA CAGTGACAGC
    CACATGGGAG AAACCATCAA AGCTGAACCA GGGTCCATCG AGGAACTACC TTGTTCCGCA
    CTAGCTCCAT CCCTAGAACC CTGCTTCTCC AGGCCCGAGA GACCAGCAAA CCGTCGCCTT
    CCGTCCCGTT GGGCCTCACA TTCCCCCACT GCCTCACTGG CTCAGTCACC CGGAGACCCA
    GCCTCCTTGG AGGAGCTCGG CGATGAGGAG CCACCGGGGG AGAAGACACA CCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe289887
    Clone ID Related Accession (Same CDS sequence) XM_022421628.1
    Accession Version XM_022421628.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4695bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product microtubule cross-linking factor 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTTGCAGG ACACAAAGAG TTCCCTGAAA AGAAGAGGCA ACCGAGAAAC ATACAAAGAA 
    AAGAAAACCT TTAACCAGCA GAGTGGTGGA ACCGAGCGCC TTGGAAACTG CAGGTCTACA
    ACCAAAACTC AAAGGAAACT GGCACCCCGG CGCAAGGATG ACAGTGCCGA TTTGAAGTGC
    CAGCTTCAGT TTGCCAAAGA GGAAGGCTCC CTGATGCGTA AAAAGATGGC CAAGCTGGGG
    AAGGAGAAGG ACGAGCTGGA ACAGGAGCTC CAGAAGTACA AGGCCCTCTA TGGAGACGTG
    GACAGCCCCC TCCCCACGGG GGAAGCCGGG GGGCCGCCCA GCACCAGGGA GGCTGAGCTG
    AAGCTGCGCT TGAAGTTGGT GGAGGAAGAG GCCAACATCT TGGGCCGGAA GATTGTGGAA
    CTGGAGGTGG AGAACCGAGG CCTCAAAGCA GAAATGGAGG ACATGAGGCT CCAGTACGAG
    CGAGAGGGTA CAGGCCGGGA CCACATCACA GGCATTCCTA CCTCACCCTT CGGTGACTCC
    CTGGAGTCCT CGACGGAGCT CCGCCGGCAC CTGCAGTTCG TGGAGGAGGA AGCGGAGCTG
    CTCCGGAGGT CCATATCTGA GATCGAAGAC CACAACCGGC AGCTGACCCA TGAGCTGAAC
    AAGTTCAAGT TTGAGCCTCG GCCGGAGCCA GGGTGGCTGG CGGATGGCGC GGGCAAGGGC
    GTGGGCAGCG GGGCCCCTCT GCAGGAGGAG CTCAAGTCGG CACGGCTACA GATCAATGAG
    CTCAGTGGGA AGGTGCTGAA GCTGCAGTAT GAGAACCGGG TGCTACTGTC CAATGTGCAG
    CGCTATGACC TGGCTGCCCA CCTAGGCCTG CGTGCCCCCA GCCCCCGGGA CAGCGACGCA
    GACAGCGACC AGGGCAAGAA GGAGAGCGAT GGGGAGGAAG GCCGCCAGCC CCAGCCCAAG
    CGGGAGGGCC CTATTGGCGG GGAGAGCGAC TCAGAGGAGA TGTGCGAGAA GACGTCGGGC
    TTCGGCAGCG GAAAGCCATC GGAGGCGAGC GAGCCATGTC CCGCCGAGCT CCTGCGGGCC
    AGGGAGGACA CCGAGTGCCT GGCGAGCATA AAGCACGAGG CTGAGCGCCT GGAGCGAACA
    GTGGAGCGCC TCATCACGGA CACCGCGGGC TTCGGGGACG ATGTGGGGCT GCTGGGCAGT
    GGCGAGCCCT TGCCAGGGCC CGACATCCAG GGGGCGGGGG GGACGGGCGA GGGGGGCAAG
    AAGGAGCCCC CACTGCTGGG GACCATCAAC TCGAGGATGA AGGCTTTCAG GAAGGAGCTG
    CAGGCCTTCC TGGAGCAGGT GAACCGAATC GGGGATGGCC TGCGGGAGCG CCTGGAAGGC
    CTGTCCCCCC TGCCTCACCT CACAGAGTCT TCCAGCTTTC TCTCCACCGT CACTTCCGTG
    TCCCGGGACT CCCCCGTCGG GAACCTGGGG AAGGAGCTGG TCCCGGACCT GCAGTCCAAA
    CTGAGAGGTC AGCTGGAGTG GCAGCCGGGG CAGGAGTGCG GGGACGAGCC AGAGCAGCTG
    CACCGCCGCG CTGATGGGGA CGTCGGGAGC TGCCGGCAGG GAGGCCAGAG CTGCTTCAGT
    CTAGAGAGTG TATCGCTAGA GGAGCTGCAG GGTCAGCTTG CGCAGGCGAC CAGACTGCAT
    CAAGAGGAGA CAGAGAAATT TACAAACAAG ATTCGGAAGA TGGAGGAGGA GCACCTGTAC
    GCCTTGAGGT GGAAAGAACT GGAAATGCAC AGCCTGGCTT TGCAGAACAC CCTCCATGAG
    CAAACCTGGA GTGATGAGAG GAACCTGATG CAGCAGGAGC TCCGGTCTAT GAAGCAGAAC
    ATTTTCCTTT TCTACGTCAA ACTCAGATGG CTGCTGAGGC ACTGGCGACA AGGGAAGCAG
    ATGGAGGAAG AAGGCGAGGA TTTCACTGAG AGCGAGCATC CAGAGACCCT CCCCAGGCTT
    GGTGAGCTTG GAGTGCAGGG GGACCCGAAG GGGGACGGCC CAGACCAAGA TGACAACGGT
    CCGGGCTGCG GCTTTCCGCT GGGAGAGCAT CCTCCACACT CCCCTGTGCA GATTGGTGAC
    CATGGATCGA GACTACAGCC TACACATGGG AGACAGCTCC ACAGGCAGGT GGTGGAAAAC
    CAGCAGCTGT TCAGTGCACT CAAGAGCCTG CTGGAGGACC TGCGCGCCGA GCTGCGGGAG
    GACGAGCATG CACGGCGGCG GCTGCAGCAG CAGTACGCCA GCGACAAAGC CGCCTGGGAC
    GTGGAGTGGG CTGCACTTAC CTGCCGCCTG GAACAGGTGC CTGCCTGCGG GGTCTGTCCT
    GGGTCTCCCC AGCTCCTCCT CTGCCATCTG GGCCAGCTGG AAGAGAAGAC TGAGAAGAAT
    TTGGGAGAAG CATGCTCTTC CACCGACAGC AAAGGGGCAT TCAAGAAGGA AAGAGAGATG
    CATCAGAAGC TCCTCACTGA CAGTCATGGC CTGGTCATGG ACCTGCGCTG GCAGATCCAT
    CACAGTGAGA AGAACTGGAG CCGGGAGAAG GTGGAACTTC TTAGCCGTCT AGACAGAGAC
    CGGCAGGAAT GGGAGCAGCA GAAGAAGGAG CTCTTGTGGA GAATAGAACA GTTGCAGAAA
    GAAAACAGTC CCCGGAGAAG TGGCAGTTTC CTCTGTGATC AAAAAGAAGG CACCATCCGC
    CCCTTTGCCC ATCCAGGAAG CCCCCGAATG CCCCGTCCCT TGGGGATGTG GTCCTGTGCA
    GACACCGACT CCACCCCATT CGAAGACCGG CCGCTGTCCA AGCTGAAGGA ATCAGACAGA
    TGTTCAGCAC GGGAAAACCT CTACTTGGAC GCCTTGTCTC TAGATGACGA GCCAGAGGAG
    CCTCCAGCCC GAGAGCCCCA GCGGGAGTTC AGGAACTGCC TCCCCCAGGA AGAAGAAAAT
    CACAAGGGAA ATCTTCAAAG GGCCGTGTCT GTGTCCTCCA TGTCTGAGTT CCAGCGTCTG
    ATGGACGTTT CTCCCTTCCT GCCGGAGAAG GGCGTCCCCG CCACCAGCAG CAAGGAGGAC
    ATCACTCCGC CCCTGTCTCC TGATGATCTG AAGTACATCG AGGAGTTCAA CAGCAAGAAC
    TGGGACGAGA GGCCCCCGGA GCCCTGGGCA GACAGGACCG AGGGGGGGCG GGCAGGGAGC
    GAGGCTGGTG CAGAGCCTTT CCCTGACTCC TCCTGGTACC TCACCACGAG TGTCACCATG
    ACTACGGACA CCATGACCAG CCCAGAGCAC TGCCAGAAGC AGCCCCTTCG GAGCCCCATG
    TGCGCGGAGC AGGCCGGGGT GCGGGTGCTG CACAGCCCGC CCGCGGTCCG TAGGATGGAT
    GGCATCGTGG TGGCGGGCAG TGAGGGCAAG GGCCGGGCCG AGCCCGAGTG CCCGTTTCCA
    ACTAGCAGAG CCAGGGGGGG CACTGGAGAC ACAAAAGGGG GTCCCTCAGA ACACGTGCTC
    AGCAGGTGGC CTTGCGCCCC CTCCAGGCAC CCCCGAGACT ATGTGGAGGG AGGGCTGCGC
    CCCCTCGAAA GTGCCCTCTG CGCCCCCCTG GGTTTTGCCT CCTCGCTGCA CGGCCTGCAG
    ATGTCCAAGA ACATGAGTGA TGACATGAAG GAGGTGGCCT TCTCGGTCAG GAACGCCATC
    TGCTCTGGCC CCCCCAAGCC TCCGCTCAAG GACATGGCCT GCCAGACCAA TGGGTCCCGG
    ACGACGGGAA CACAGACCGT CCAGACCATC ACCATTGGCC TGCAGACCGA AGCCCTGCGT
    GGCAGCGCCG TCACCAGCAG CCCCCACAAG TGTCTCACAC CCAAGGCCGG GGGCGGGGCC
    ACGCCCGCGT CATCTCCTTC CCGTGGCCTT CGGAGCCGGC AGATGGCCCC CGCCATCGAG
    AAGGTGCAGG CCAAGTTCGA ACGCACGTGC TGCTCCCCCA AGTACGGGTC TCCCAAGCTG
    CAGAGGAAGC CCCTCCCCAA AGCTGAGCCG CCCAACAGCA GGACCTCCCT GGGGCTGGCC
    CCAAAGGGGT GCAGCGAGTC CGCCTGGGCC CGTTCCACCA CCACGAGGGA GAGCCCGGTG
    CACACCGCCA TCAACGATGG CCTCTCCAGC CTCTTCAACA TCATCGACCA CAGCCCCGTG
    GTGCAGGACC CCTTCCAGAA GGGGACTCGG GCCGGGAGCC GATCTCGCTC AGCAGAACCC
    CGGCCCGAGC TGGGCCCAGG CCAGGAAACG GGCCCCAGTT CCCGGGGCCG GTCCCCCAGC
    CCCATCGGGG CTGGCTCAGA GACTTGGAGG GAGGAAGGGG GAGAGGGCAC GCCCTTGAGG
    CAGGACTTAT CCGCTCCCCC TGGTTACACG TTCACTGAGA ACGTAGCCCG GATCCTCAAC
    AAGAAGCTGT TGGAGCATGC CTTAAAGGAG GAGAGGAGGC AGGCCCCCCA CGGGCCCCCA
    AGTCTTAACA GTGACAGCCA CATGGGAGAA ACCATCAAAG CTGAACCAGG GTCCATCGAG
    GAACTACCTT GTTCCGCACT AGCTCCATCC CTAGAACCCT GCTTCTCCAG GCCCGAGAGA
    CCAGCAAACC GTCGCCTTCC GTCCCGTTGG GCCTCACATT CCCCCACTGC CTCACTGGCT
    CAGTCACCCG GAGACCCAGC CTCCTTGGAG GAGCTCGGCG ATGAGGAGCC ACCGGGGGAG
    AAGACACACC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022277336.1
    CDS699..5393
    Translation

    Target ORF information:

    RefSeq Version XM_022421628.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022421628.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTTGCAGG ACACAAAGAG TTCCCTGAAA AGAAGAGGCA ACCGAGAAAC ATACAAAGAA 
    AAGAAAACCT TTAACCAGCA GAGTGGTGGA ACCGAGCGCC TTGGAAACTG CAGGTCTACA
    ACCAAAACTC AAAGGAAACT GGCACCCCGG CGCAAGGATG ACAGTGCCGA TTTGAAGTGC
    CAGCTTCAGT TTGCCAAAGA GGAAGGCTCC CTGATGCGTA AAAAGATGGC CAAGCTGGGG
    AAGGAGAAGG ACGAGCTGGA ACAGGAGCTC CAGAAGTACA AGGCCCTCTA TGGAGACGTG
    GACAGCCCCC TCCCCACGGG GGAAGCCGGG GGGCCGCCCA GCACCAGGGA GGCTGAGCTG
    AAGCTGCGCT TGAAGTTGGT GGAGGAAGAG GCCAACATCT TGGGCCGGAA GATTGTGGAA
    CTGGAGGTGG AGAACCGAGG CCTCAAAGCA GAAATGGAGG ACATGAGGCT CCAGTACGAG
    CGAGAGGGTA CAGGCCGGGA CCACATCACA GGCATTCCTA CCTCACCCTT CGGTGACTCC
    CTGGAGTCCT CGACGGAGCT CCGCCGGCAC CTGCAGTTCG TGGAGGAGGA AGCGGAGCTG
    CTCCGGAGGT CCATATCTGA GATCGAAGAC CACAACCGGC AGCTGACCCA TGAGCTGAAC
    AAGTTCAAGT TTGAGCCTCG GCCGGAGCCA GGGTGGCTGG CGGATGGCGC GGGCAAGGGC
    GTGGGCAGCG GGGCCCCTCT GCAGGAGGAG CTCAAGTCGG CACGGCTACA GATCAATGAG
    CTCAGTGGGA AGGTGCTGAA GCTGCAGTAT GAGAACCGGG TGCTACTGTC CAATGTGCAG
    CGCTATGACC TGGCTGCCCA CCTAGGCCTG CGTGCCCCCA GCCCCCGGGA CAGCGACGCA
    GACAGCGACC AGGGCAAGAA GGAGAGCGAT GGGGAGGAAG GCCGCCAGCC CCAGCCCAAG
    CGGGAGGGCC CTATTGGCGG GGAGAGCGAC TCAGAGGAGA TGTGCGAGAA GACGTCGGGC
    TTCGGCAGCG GAAAGCCATC GGAGGCGAGC GAGCCATGTC CCGCCGAGCT CCTGCGGGCC
    AGGGAGGACA CCGAGTGCCT GGCGAGCATA AAGCACGAGG CTGAGCGCCT GGAGCGAACA
    GTGGAGCGCC TCATCACGGA CACCGCGGGC TTCGGGGACG ATGTGGGGCT GCTGGGCAGT
    GGCGAGCCCT TGCCAGGGCC CGACATCCAG GGGGCGGGGG GGACGGGCGA GGGGGGCAAG
    AAGGAGCCCC CACTGCTGGG GACCATCAAC TCGAGGATGA AGGCTTTCAG GAAGGAGCTG
    CAGGCCTTCC TGGAGCAGGT GAACCGAATC GGGGATGGCC TGCGGGAGCG CCTGGAAGGC
    CTGTCCCCCC TGCCTCACCT CACAGAGTCT TCCAGCTTTC TCTCCACCGT CACTTCCGTG
    TCCCGGGACT CCCCCGTCGG GAACCTGGGG AAGGAGCTGG TCCCGGACCT GCAGTCCAAA
    CTGAGAGGTC AGCTGGAGTG GCAGCCGGGG CAGGAGTGCG GGGACGAGCC AGAGCAGCTG
    CACCGCCGCG CTGATGGGGA CGTCGGGAGC TGCCGGCAGG GAGGCCAGAG CTGCTTCAGT
    CTAGAGAGTG TATCGCTAGA GGAGCTGCAG GGTCAGCTTG CGCAGGCGAC CAGACTGCAT
    CAAGAGGAGA CAGAGAAATT TACAAACAAG ATTCGGAAGA TGGAGGAGGA GCACCTGTAC
    GCCTTGAGGT GGAAAGAACT GGAAATGCAC AGCCTGGCTT TGCAGAACAC CCTCCATGAG
    CAAACCTGGA GTGATGAGAG GAACCTGATG CAGCAGGAGC TCCGGTCTAT GAAGCAGAAC
    ATTTTCCTTT TCTACGTCAA ACTCAGATGG CTGCTGAGGC ACTGGCGACA AGGGAAGCAG
    ATGGAGGAAG AAGGCGAGGA TTTCACTGAG AGCGAGCATC CAGAGACCCT CCCCAGGCTT
    GGTGAGCTTG GAGTGCAGGG GGACCCGAAG GGGGACGGCC CAGACCAAGA TGACAACGGT
    CCGGGCTGCG GCTTTCCGCT GGGAGAGCAT CCTCCACACT CCCCTGTGCA GATTGGTGAC
    CATGGATCGA GACTACAGCC TACACATGGG AGACAGCTCC ACAGGCAGGT GGTGGAAAAC
    CAGCAGCTGT TCAGTGCACT CAAGAGCCTG CTGGAGGACC TGCGCGCCGA GCTGCGGGAG
    GACGAGCATG CACGGCGGCG GCTGCAGCAG CAGTACGCCA GCGACAAAGC CGCCTGGGAC
    GTGGAGTGGG CTGCACTTAC CTGCCGCCTG GAACAGGTGC CTGCCTGCGG GGTCTGTCCT
    GGGTCTCCCC AGCTCCTCCT CTGCCATCTG GGCCAGCTGG AAGAGAAGAC TGAGAAGAAT
    TTGGGAGAAG CATGCTCTTC CACCGACAGC AAAGGGGCAT TCAAGAAGGA AAGAGAGATG
    CATCAGAAGC TCCTCACTGA CAGTCATGGC CTGGTCATGG ACCTGCGCTG GCAGATCCAT
    CACAGTGAGA AGAACTGGAG CCGGGAGAAG GTGGAACTTC TTAGCCGTCT AGACAGAGAC
    CGGCAGGAAT GGGAGCAGCA GAAGAAGGAG CTCTTGTGGA GAATAGAACA GTTGCAGAAA
    GAAAACAGTC CCCGGAGAAG TGGCAGTTTC CTCTGTGATC AAAAAGAAGG CACCATCCGC
    CCCTTTGCCC ATCCAGGAAG CCCCCGAATG CCCCGTCCCT TGGGGATGTG GTCCTGTGCA
    GACACCGACT CCACCCCATT CGAAGACCGG CCGCTGTCCA AGCTGAAGGA ATCAGACAGA
    TGTTCAGCAC GGGAAAACCT CTACTTGGAC GCCTTGTCTC TAGATGACGA GCCAGAGGAG
    CCTCCAGCCC GAGAGCCCCA GCGGGAGTTC AGGAACTGCC TCCCCCAGGA AGAAGAAAAT
    CACAAGGGAA ATCTTCAAAG GGCCGTGTCT GTGTCCTCCA TGTCTGAGTT CCAGCGTCTG
    ATGGACGTTT CTCCCTTCCT GCCGGAGAAG GGCGTCCCCG CCACCAGCAG CAAGGAGGAC
    ATCACTCCGC CCCTGTCTCC TGATGATCTG AAGTACATCG AGGAGTTCAA CAGCAAGAAC
    TGGGACGAGA GGCCCCCGGA GCCCTGGGCA GACAGGACCG AGGGGGGGCG GGCAGGGAGC
    GAGGCTGGTG CAGAGCCTTT CCCTGACTCC TCCTGGTACC TCACCACGAG TGTCACCATG
    ACTACGGACA CCATGACCAG CCCAGAGCAC TGCCAGAAGC AGCCCCTTCG GAGCCCCATG
    TGCGCGGAGC AGGCCGGGGT GCGGGTGCTG CACAGCCCGC CCGCGGTCCG TAGGATGGAT
    GGCATCGTGG TGGCGGGCAG TGAGGGCAAG GGCCGGGCCG AGCCCGAGTG CCCGTTTCCA
    ACTAGCAGAG CCAGGGGGGG CACTGGAGAC ACAAAAGGGG GTCCCTCAGA ACACGTGCTC
    AGCAGGTGGC CTTGCGCCCC CTCCAGGCAC CCCCGAGACT ATGTGGAGGG AGGGCTGCGC
    CCCCTCGAAA GTGCCCTCTG CGCCCCCCTG GGTTTTGCCT CCTCGCTGCA CGGCCTGCAG
    ATGTCCAAGA ACATGAGTGA TGACATGAAG GAGGTGGCCT TCTCGGTCAG GAACGCCATC
    TGCTCTGGCC CCCCCAAGCC TCCGCTCAAG GACATGGCCT GCCAGACCAA TGGGTCCCGG
    ACGACGGGAA CACAGACCGT CCAGACCATC ACCATTGGCC TGCAGACCGA AGCCCTGCGT
    GGCAGCGCCG TCACCAGCAG CCCCCACAAG TGTCTCACAC CCAAGGCCGG GGGCGGGGCC
    ACGCCCGCGT CATCTCCTTC CCGTGGCCTT CGGAGCCGGC AGATGGCCCC CGCCATCGAG
    AAGGTGCAGG CCAAGTTCGA ACGCACGTGC TGCTCCCCCA AGTACGGGTC TCCCAAGCTG
    CAGAGGAAGC CCCTCCCCAA AGCTGAGCCG CCCAACAGCA GGACCTCCCT GGGGCTGGCC
    CCAAAGGGGT GCAGCGAGTC CGCCTGGGCC CGTTCCACCA CCACGAGGGA GAGCCCGGTG
    CACACCGCCA TCAACGATGG CCTCTCCAGC CTCTTCAACA TCATCGACCA CAGCCCCGTG
    GTGCAGGACC CCTTCCAGAA GGGGACTCGG GCCGGGAGCC GATCTCGCTC AGCAGAACCC
    CGGCCCGAGC TGGGCCCAGG CCAGGAAACG GGCCCCAGTT CCCGGGGCCG GTCCCCCAGC
    CCCATCGGGG CTGGCTCAGA GACTTGGAGG GAGGAAGGGG GAGAGGGCAC GCCCTTGAGG
    CAGGACTTAT CCGCTCCCCC TGGTTACACG TTCACTGAGA ACGTAGCCCG GATCCTCAAC
    AAGAAGCTGT TGGAGCATGC CTTAAAGGAG GAGAGGAGGC AGGCCCCCCA CGGGCCCCCA
    AGTCTTAACA GTGACAGCCA CATGGGAGAA ACCATCAAAG CTGAACCAGG GTCCATCGAG
    GAACTACCTT GTTCCGCACT AGCTCCATCC CTAGAACCCT GCTTCTCCAG GCCCGAGAGA
    CCAGCAAACC GTCGCCTTCC GTCCCGTTGG GCCTCACATT CCCCCACTGC CTCACTGGCT
    CAGTCACCCG GAGACCCAGC CTCCTTGGAG GAGCTCGGCG ATGAGGAGCC ACCGGGGGAG
    AAGACACACC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe289888
    Clone ID Related Accession (Same CDS sequence) XM_022421629.1
    Accession Version XM_022421629.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4866bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product microtubule cross-linking factor 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG
    CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG
    TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG
    CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC
    ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG
    GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT
    CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG
    TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC
    CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG
    CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG
    TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC
    CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC
    CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG
    GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG
    GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA
    TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC
    GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG
    GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG
    GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG
    ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT
    GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC
    TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG
    CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG
    TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG
    CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG ATGGAGGAGG AGCACCTGTA CGCCTTGAGG
    TGGAAAGAAC TGGAAATGCA CAGCCTGGCT TTGCAGAACA CCCTCCATGA GCAAACCTGG
    AGTGATGAGA GGAACCTGAT GCAGCAGGAG CTCCGGTCTA TGAAGCAGAA CATTTTCCTT
    TTCTACGTCA AACTCAGATG GCTGCTGAGG CACTGGCGAC AAGGGAAGCA GATGGAGGAA
    GAAGGCGAGG ATTTCACTGA GAGCGAGCAT CCAGAGACCC TCCCCAGGCT TGGTGAGCTT
    GGAGTGCAGG GGGACCCGAA GGGGGACGGC CCAGACCAAG ATGACAACGG TCCGGGCTGC
    GGCTTTCCGC TGGGAGAGCA TCCTCCACAC TCCCCTGTGC AGATTGGTGA CCATGGATCG
    AGACTACAGC CTACACATGG GAGACAGCTC CACAGGCAGG TGGTGGAAAA CCAGCAGCTG
    TTCAGTGCAC TCAAGAGCCT GCTGGAGGAC CTGCGCGCCG AGCTGCGGGA GGACGAGCAT
    GCACGGCGGC GGCTGCAGCA GCAGTACGCC AGCGACAAAG CCGCCTGGGA CGTGGAGTGG
    GCTGCACTTA CCTGCCGCCT GGAACAGCTG GAAGAGAAGA CTGAGAAGAA TTTGGGAGAA
    GCATGCTCTT CCACCGACAG CAAAGGGGCA TTCAAGAAGG AAAGAGAGAT GCATCAGAAG
    CTCCTCACTG ACAGTCATGG CCTGGTCATG GACCTGCGCT GGCAGATCCA TCACAGTGAG
    AAGAACTGGA GCCGGGAGAA GGTGGAACTT CTTAGCCGTC TAGACAGAGA CCGGCAGGAA
    TGGGAGCAGC AGAAGAAGGA GCTCTTGTGG AGAATAGAAC AGTTGCAGAA AGAAAACAGT
    CCCCGGAGAA GTGGCAGTTT CCTCTGTGAT CAAAAAGAAG GCACCATCCG CCCCTTTGCC
    CATCCAGGAA GCCCCCGAAT GCCCCGTCCC TTGGGGATGT GGTCCTGTGC AGACACCGAC
    TCCACCCCAT TCGAAGACCG GCCGCTGTCC AAGCTGAAGG AATCAGACAG ATGTTCAGCA
    CGGGAAAACC TCTACTTGGA CGCCTTGTCT CTAGATGACG AGCCAGAGGA GCCTCCAGCC
    CGAGAGCCCC AGCGGGAGTT CAGGAACTGC CTCCCCCAGG AAGAAGAAAA TCACAAGGGA
    AATCTTCAAA GGGCCGTGTC TGTGTCCTCC ATGTCTGAGT TCCAGCGTCT GATGGACGTT
    TCTCCCTTCC TGCCGGAGAA GGGCGTCCCC GCCACCAGCA GCAAGGAGGA CATCACTCCG
    CCCCTGTCTC CTGATGATCT GAAGTACATC GAGGAGTTCA ACAGCAAGAA CTGGGACGAG
    AGGCCCCCGG AGCCCTGGGC AGACAGGACC GAGGGGGGGC GGGCAGGGAG CGAGGCTGGT
    GCAGAGCCTT TCCCTGACTC CTCCTGGTAC CTCACCACGA GTGTCACCAT GACTACGGAC
    ACCATGACCA GCCCAGAGCA CTGCCAGAAG CAGCCCCTTC GGAGCCCCAT GTGCGCGGAG
    CAGGCCGGGG TGCGGGTGCT GCACAGCCCG CCCGCGGTCC GTAGGATGGA TGGCATCGTG
    GTGGCGGGCA GTGAGGGCAA GGGCCGGGCC GAGCCCGAGT GCCCGTTTCC AACTAGCAGA
    GCCAGGGGGG GCACTGGAGA CACAAAAGGG GGTCCCTCAG AACACGTGCT CAGCAGGTGG
    CCTTGCGCCC CCTCCAGGCA CCCCCGAGAC TATGTGGAGG GAGGGCTGCG CCCCCTCGAA
    AGTGCCCTCT GCGCCCCCCT GGGTTTTGCC TCCTCGCTGC ACGGCCTGCA GATGTCCAAG
    AACATGAGTG ATGACATGAA GGAGGTGGCC TTCTCGGTCA GGAACGCCAT CTGCTCTGGC
    CCCCCCAAGC CTCCGCTCAA GGACATGGCC TGCCAGACCA ATGGGTCCCG GACGACGGGA
    ACACAGACCG TCCAGACCAT CACCATTGGC CTGCAGACCG AAGCCCTGCG TGGCAGCGCC
    GTCACCAGCA GCCCCCACAA GTGTCTCACA CCCAAGGCCG GGGGCGGGGC CACGCCCGCG
    TCATCTCCTT CCCGTGGCCT TCGGAGCCGG CAGATGGCCC CCGCCATCGA GAAGGTGCAG
    GCCAAGTTCG AACGCACGTG CTGCTCCCCC AAGTACGGGT CTCCCAAGCT GCAGAGGAAG
    CCCCTCCCCA AAGCTGAGCC GCCCAACAGC AGGACCTCCC TGGGGCTGGC CCCAAAGGGG
    TGCAGCGAGT CCGCCTGGGC CCGTTCCACC ACCACGAGGG AGAGCCCGGT GCACACCGCC
    ATCAACGATG GCCTCTCCAG CCTCTTCAAC ATCATCGACC ACAGCCCCGT GGTGCAGGAC
    CCCTTCCAGA AGGGGACTCG GGCCGGGAGC CGATCTCGCT CAGCAGAACC CCGGCCCGAG
    CTGGGCCCAG GCCAGGAAAC GGGCCCCAGT TCCCGGGGCC GGTCCCCCAG CCCCATCGGG
    GCTGGCTCAG AGACTTGGAG GGAGGAAGGG GGAGAGGGCA CGCCCTTGAG GCAGGACTTA
    TCCGCTCCCC CTGGTTACAC GTTCACTGAG AACGTAGCCC GGATCCTCAA CAAGAAGCTG
    TTGGAGCATG CCTTAAAGGA GGAGAGGAGG CAGGCCCCCC ACGGGCCCCC AAGTCTTAAC
    AGTGACAGCC ACATGGGAGA AACCATCAAA GCTGAACCAG GGTCCATCGA GGAACTACCT
    TGTTCCGCAC TAGCTCCATC CCTAGAACCC TGCTTCTCCA GGCCCGAGAG ACCAGCAAAC
    CGTCGCCTTC CGTCCCGTTG GGCCTCACAT TCCCCCACTG CCTCACTGGC TCAGTCACCC
    GGAGACCCAG CCTCCTTGGA GGAGCTCGGC GATGAGGAGC CACCGGGGGA GAAGACACAC
    CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022277337.1
    CDS128..4993
    Translation

    Target ORF information:

    RefSeq Version XM_022421629.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022421629.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG
    CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG
    TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG
    CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC
    ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG
    GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT
    CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG
    TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC
    CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG
    CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG
    TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC
    CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC
    CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG
    GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG
    GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA
    TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC
    GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG
    GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG
    GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG
    ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT
    GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC
    TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG
    CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG
    TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG
    CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG ATGGAGGAGG AGCACCTGTA CGCCTTGAGG
    TGGAAAGAAC TGGAAATGCA CAGCCTGGCT TTGCAGAACA CCCTCCATGA GCAAACCTGG
    AGTGATGAGA GGAACCTGAT GCAGCAGGAG CTCCGGTCTA TGAAGCAGAA CATTTTCCTT
    TTCTACGTCA AACTCAGATG GCTGCTGAGG CACTGGCGAC AAGGGAAGCA GATGGAGGAA
    GAAGGCGAGG ATTTCACTGA GAGCGAGCAT CCAGAGACCC TCCCCAGGCT TGGTGAGCTT
    GGAGTGCAGG GGGACCCGAA GGGGGACGGC CCAGACCAAG ATGACAACGG TCCGGGCTGC
    GGCTTTCCGC TGGGAGAGCA TCCTCCACAC TCCCCTGTGC AGATTGGTGA CCATGGATCG
    AGACTACAGC CTACACATGG GAGACAGCTC CACAGGCAGG TGGTGGAAAA CCAGCAGCTG
    TTCAGTGCAC TCAAGAGCCT GCTGGAGGAC CTGCGCGCCG AGCTGCGGGA GGACGAGCAT
    GCACGGCGGC GGCTGCAGCA GCAGTACGCC AGCGACAAAG CCGCCTGGGA CGTGGAGTGG
    GCTGCACTTA CCTGCCGCCT GGAACAGCTG GAAGAGAAGA CTGAGAAGAA TTTGGGAGAA
    GCATGCTCTT CCACCGACAG CAAAGGGGCA TTCAAGAAGG AAAGAGAGAT GCATCAGAAG
    CTCCTCACTG ACAGTCATGG CCTGGTCATG GACCTGCGCT GGCAGATCCA TCACAGTGAG
    AAGAACTGGA GCCGGGAGAA GGTGGAACTT CTTAGCCGTC TAGACAGAGA CCGGCAGGAA
    TGGGAGCAGC AGAAGAAGGA GCTCTTGTGG AGAATAGAAC AGTTGCAGAA AGAAAACAGT
    CCCCGGAGAA GTGGCAGTTT CCTCTGTGAT CAAAAAGAAG GCACCATCCG CCCCTTTGCC
    CATCCAGGAA GCCCCCGAAT GCCCCGTCCC TTGGGGATGT GGTCCTGTGC AGACACCGAC
    TCCACCCCAT TCGAAGACCG GCCGCTGTCC AAGCTGAAGG AATCAGACAG ATGTTCAGCA
    CGGGAAAACC TCTACTTGGA CGCCTTGTCT CTAGATGACG AGCCAGAGGA GCCTCCAGCC
    CGAGAGCCCC AGCGGGAGTT CAGGAACTGC CTCCCCCAGG AAGAAGAAAA TCACAAGGGA
    AATCTTCAAA GGGCCGTGTC TGTGTCCTCC ATGTCTGAGT TCCAGCGTCT GATGGACGTT
    TCTCCCTTCC TGCCGGAGAA GGGCGTCCCC GCCACCAGCA GCAAGGAGGA CATCACTCCG
    CCCCTGTCTC CTGATGATCT GAAGTACATC GAGGAGTTCA ACAGCAAGAA CTGGGACGAG
    AGGCCCCCGG AGCCCTGGGC AGACAGGACC GAGGGGGGGC GGGCAGGGAG CGAGGCTGGT
    GCAGAGCCTT TCCCTGACTC CTCCTGGTAC CTCACCACGA GTGTCACCAT GACTACGGAC
    ACCATGACCA GCCCAGAGCA CTGCCAGAAG CAGCCCCTTC GGAGCCCCAT GTGCGCGGAG
    CAGGCCGGGG TGCGGGTGCT GCACAGCCCG CCCGCGGTCC GTAGGATGGA TGGCATCGTG
    GTGGCGGGCA GTGAGGGCAA GGGCCGGGCC GAGCCCGAGT GCCCGTTTCC AACTAGCAGA
    GCCAGGGGGG GCACTGGAGA CACAAAAGGG GGTCCCTCAG AACACGTGCT CAGCAGGTGG
    CCTTGCGCCC CCTCCAGGCA CCCCCGAGAC TATGTGGAGG GAGGGCTGCG CCCCCTCGAA
    AGTGCCCTCT GCGCCCCCCT GGGTTTTGCC TCCTCGCTGC ACGGCCTGCA GATGTCCAAG
    AACATGAGTG ATGACATGAA GGAGGTGGCC TTCTCGGTCA GGAACGCCAT CTGCTCTGGC
    CCCCCCAAGC CTCCGCTCAA GGACATGGCC TGCCAGACCA ATGGGTCCCG GACGACGGGA
    ACACAGACCG TCCAGACCAT CACCATTGGC CTGCAGACCG AAGCCCTGCG TGGCAGCGCC
    GTCACCAGCA GCCCCCACAA GTGTCTCACA CCCAAGGCCG GGGGCGGGGC CACGCCCGCG
    TCATCTCCTT CCCGTGGCCT TCGGAGCCGG CAGATGGCCC CCGCCATCGA GAAGGTGCAG
    GCCAAGTTCG AACGCACGTG CTGCTCCCCC AAGTACGGGT CTCCCAAGCT GCAGAGGAAG
    CCCCTCCCCA AAGCTGAGCC GCCCAACAGC AGGACCTCCC TGGGGCTGGC CCCAAAGGGG
    TGCAGCGAGT CCGCCTGGGC CCGTTCCACC ACCACGAGGG AGAGCCCGGT GCACACCGCC
    ATCAACGATG GCCTCTCCAG CCTCTTCAAC ATCATCGACC ACAGCCCCGT GGTGCAGGAC
    CCCTTCCAGA AGGGGACTCG GGCCGGGAGC CGATCTCGCT CAGCAGAACC CCGGCCCGAG
    CTGGGCCCAG GCCAGGAAAC GGGCCCCAGT TCCCGGGGCC GGTCCCCCAG CCCCATCGGG
    GCTGGCTCAG AGACTTGGAG GGAGGAAGGG GGAGAGGGCA CGCCCTTGAG GCAGGACTTA
    TCCGCTCCCC CTGGTTACAC GTTCACTGAG AACGTAGCCC GGATCCTCAA CAAGAAGCTG
    TTGGAGCATG CCTTAAAGGA GGAGAGGAGG CAGGCCCCCC ACGGGCCCCC AAGTCTTAAC
    AGTGACAGCC ACATGGGAGA AACCATCAAA GCTGAACCAG GGTCCATCGA GGAACTACCT
    TGTTCCGCAC TAGCTCCATC CCTAGAACCC TGCTTCTCCA GGCCCGAGAG ACCAGCAAAC
    CGTCGCCTTC CGTCCCGTTG GGCCTCACAT TCCCCCACTG CCTCACTGGC TCAGTCACCC
    GGAGACCCAG CCTCCTTGGA GGAGCTCGGC GATGAGGAGC CACCGGGGGA GAAGACACAC
    CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe289889
    Clone ID Related Accession (Same CDS sequence) XM_022421630.1
    Accession Version XM_022421630.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4617bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product microtubule cross-linking factor 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTGCAGG ACACAAAGAG TTCCCTGAAA AGAAGAGGCA ACCGAGAAAC ATACAAAGAA 
    AAGAAAACCT TTAACCAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA
    GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG
    GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG
    GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG
    GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA
    GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG
    GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG
    CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT
    GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT
    CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT
    CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG
    AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC
    CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG
    AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC
    GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA
    TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC
    CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG
    GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG
    CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG
    GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG
    GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC
    CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC
    GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGTCCA AACTGAGAGG TCAGCTGGAG
    TGGCAGCCGG GGCAGGAGTG CGGGGACGAG CCAGAGCAGC TGCACCGCCG CGCTGATGGG
    GACGTCGGGA GCTGCCGGCA GGGAGGCCAG AGCTGCTTCA GTCTAGAGAG TGTATCGCTA
    GAGGAGCTGC AGGGTCAGCT TGCGCAGGCG ACCAGACTGC ATCAAGAGGA GACAGAGAAA
    TTTACAAACA AGATTCGGAA GATGGAGGAG GAGCACCTGT ACGCCTTGAG GTGGAAAGAA
    CTGGAAATGC ACAGCCTGGC TTTGCAGAAC ACCCTCCATG AGCAAACCTG GAGTGATGAG
    AGGAACCTGA TGCAGCAGGA GCTCCGGTCT ATGAAGCAGA ACATTTTCCT TTTCTACGTC
    AAACTCAGAT GGCTGCTGAG GCACTGGCGA CAAGGGAAGC AGATGGAGGA AGAAGGCGAG
    GATTTCACTG AGAGCGAGCA TCCAGAGACC CTCCCCAGGC TTGGTGAGCT TGGAGTGCAG
    GGGGACCCGA AGGGGGACGG CCCAGACCAA GATGACAACG GTCCGGGCTG CGGCTTTCCG
    CTGGGAGAGC ATCCTCCACA CTCCCCTGTG CAGATTGGTG ACCATGGATC GAGACTACAG
    CCTACACATG GGAGACAGCT CCACAGGCAG GTGGTGGAAA ACCAGCAGCT GTTCAGTGCA
    CTCAAGAGCC TGCTGGAGGA CCTGCGCGCC GAGCTGCGGG AGGACGAGCA TGCACGGCGG
    CGGCTGCAGC AGCAGTACGC CAGCGACAAA GCCGCCTGGG ACGTGGAGTG GGCTGCACTT
    ACCTGCCGCC TGGAACAGGT GCCTGCCTGC GGGGTCTGTC CTGGGTCTCC CCAGCTCCTC
    CTCTGCCATC TGGGCCAGCT GGAAGAGAAG ACTGAGAAGA ATTTGGGAGA AGCATGCTCT
    TCCACCGACA GCAAAGGGGC ATTCAAGAAG GAAAGAGAGA TGCATCAGAA GCTCCTCACT
    GACAGTCATG GCCTGGTCAT GGACCTGCGC TGGCAGATCC ATCACAGTGA GAAGAACTGG
    AGCCGGGAGA AGGTGGAACT TCTTAGCCGT CTAGACAGAG ACCGGCAGGA ATGGGAGCAG
    CAGAAGAAGG AGCTCTTGTG GAGAATAGAA CAGTTGCAGA AAGAAAACAG TCCCCGGAGA
    AGTGGCAGTT TCCTCTGTGA TCAAAAAGAA GGCACCATCC GCCCCTTTGC CCATCCAGGA
    AGCCCCCGAA TGCCCCGTCC CTTGGGGATG TGGTCCTGTG CAGACACCGA CTCCACCCCA
    TTCGAAGACC GGCCGCTGTC CAAGCTGAAG GAATCAGACA GATGTTCAGC ACGGGAAAAC
    CTCTACTTGG ACGCCTTGTC TCTAGATGAC GAGCCAGAGG AGCCTCCAGC CCGAGAGCCC
    CAGCGGGAGT TCAGGAACTG CCTCCCCCAG GAAGAAGAAA ATCACAAGGG AAATCTTCAA
    AGGGCCGTGT CTGTGTCCTC CATGTCTGAG TTCCAGCGTC TGATGGACGT TTCTCCCTTC
    CTGCCGGAGA AGGGCGTCCC CGCCACCAGC AGCAAGGAGG ACATCACTCC GCCCCTGTCT
    CCTGATGATC TGAAGTACAT CGAGGAGTTC AACAGCAAGA ACTGGGACGA GAGGCCCCCG
    GAGCCCTGGG CAGACAGGAC CGAGGGGGGG CGGGCAGGGA GCGAGGCTGG TGCAGAGCCT
    TTCCCTGACT CCTCCTGGTA CCTCACCACG AGTGTCACCA TGACTACGGA CACCATGACC
    AGCCCAGAGC ACTGCCAGAA GCAGCCCCTT CGGAGCCCCA TGTGCGCGGA GCAGGCCGGG
    GTGCGGGTGC TGCACAGCCC GCCCGCGGTC CGTAGGATGG ATGGCATCGT GGTGGCGGGC
    AGTGAGGGCA AGGGCCGGGC CGAGCCCGAG TGCCCGTTTC CAACTAGCAG AGCCAGGGGG
    GGCACTGGAG ACACAAAAGG GGGTCCCTCA GAACACGTGC TCAGCAGGTG GCCTTGCGCC
    CCCTCCAGGC ACCCCCGAGA CTATGTGGAG GGAGGGCTGC GCCCCCTCGA AAGTGCCCTC
    TGCGCCCCCC TGGGTTTTGC CTCCTCGCTG CACGGCCTGC AGATGTCCAA GAACATGAGT
    GATGACATGA AGGAGGTGGC CTTCTCGGTC AGGAACGCCA TCTGCTCTGG CCCCCCCAAG
    CCTCCGCTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGACGGG AACACAGACC
    GTCCAGACCA TCACCATTGG CCTGCAGACC GAAGCCCTGC GTGGCAGCGC CGTCACCAGC
    AGCCCCCACA AGTGTCTCAC ACCCAAGGCC GGGGGCGGGG CCACGCCCGC GTCATCTCCT
    TCCCGTGGCC TTCGGAGCCG GCAGATGGCC CCCGCCATCG AGAAGGTGCA GGCCAAGTTC
    GAACGCACGT GCTGCTCCCC CAAGTACGGG TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC
    AAAGCTGAGC CGCCCAACAG CAGGACCTCC CTGGGGCTGG CCCCAAAGGG GTGCAGCGAG
    TCCGCCTGGG CCCGTTCCAC CACCACGAGG GAGAGCCCGG TGCACACCGC CATCAACGAT
    GGCCTCTCCA GCCTCTTCAA CATCATCGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG
    AAGGGGACTC GGGCCGGGAG CCGATCTCGC TCAGCAGAAC CCCGGCCCGA GCTGGGCCCA
    GGCCAGGAAA CGGGCCCCAG TTCCCGGGGC CGGTCCCCCA GCCCCATCGG GGCTGGCTCA
    GAGACTTGGA GGGAGGAAGG GGGAGAGGGC ACGCCCTTGA GGCAGGACTT ATCCGCTCCC
    CCTGGTTACA CGTTCACTGA GAACGTAGCC CGGATCCTCA ACAAGAAGCT GTTGGAGCAT
    GCCTTAAAGG AGGAGAGGAG GCAGGCCCCC CACGGGCCCC CAAGTCTTAA CAGTGACAGC
    CACATGGGAG AAACCATCAA AGCTGAACCA GGGTCCATCG AGGAACTACC TTGTTCCGCA
    CTAGCTCCAT CCCTAGAACC CTGCTTCTCC AGGCCCGAGA GACCAGCAAA CCGTCGCCTT
    CCGTCCCGTT GGGCCTCACA TTCCCCCACT GCCTCACTGG CTCAGTCACC CGGAGACCCA
    GCCTCCTTGG AGGAGCTCGG CGATGAGGAG CCACCGGGGG AGAAGACACA CCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022277338.1
    CDS698..5314
    Translation

    Target ORF information:

    RefSeq Version XM_022421630.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022421630.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTGCAGG ACACAAAGAG TTCCCTGAAA AGAAGAGGCA ACCGAGAAAC ATACAAAGAA 
    AAGAAAACCT TTAACCAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA
    GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG
    GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG
    GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG
    GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA
    GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG
    GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG
    CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT
    GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT
    CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT
    CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG
    AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC
    CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG
    AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC
    GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA
    TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC
    CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG
    GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG
    CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG
    GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG
    GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC
    CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC
    GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGTCCA AACTGAGAGG TCAGCTGGAG
    TGGCAGCCGG GGCAGGAGTG CGGGGACGAG CCAGAGCAGC TGCACCGCCG CGCTGATGGG
    GACGTCGGGA GCTGCCGGCA GGGAGGCCAG AGCTGCTTCA GTCTAGAGAG TGTATCGCTA
    GAGGAGCTGC AGGGTCAGCT TGCGCAGGCG ACCAGACTGC ATCAAGAGGA GACAGAGAAA
    TTTACAAACA AGATTCGGAA GATGGAGGAG GAGCACCTGT ACGCCTTGAG GTGGAAAGAA
    CTGGAAATGC ACAGCCTGGC TTTGCAGAAC ACCCTCCATG AGCAAACCTG GAGTGATGAG
    AGGAACCTGA TGCAGCAGGA GCTCCGGTCT ATGAAGCAGA ACATTTTCCT TTTCTACGTC
    AAACTCAGAT GGCTGCTGAG GCACTGGCGA CAAGGGAAGC AGATGGAGGA AGAAGGCGAG
    GATTTCACTG AGAGCGAGCA TCCAGAGACC CTCCCCAGGC TTGGTGAGCT TGGAGTGCAG
    GGGGACCCGA AGGGGGACGG CCCAGACCAA GATGACAACG GTCCGGGCTG CGGCTTTCCG
    CTGGGAGAGC ATCCTCCACA CTCCCCTGTG CAGATTGGTG ACCATGGATC GAGACTACAG
    CCTACACATG GGAGACAGCT CCACAGGCAG GTGGTGGAAA ACCAGCAGCT GTTCAGTGCA
    CTCAAGAGCC TGCTGGAGGA CCTGCGCGCC GAGCTGCGGG AGGACGAGCA TGCACGGCGG
    CGGCTGCAGC AGCAGTACGC CAGCGACAAA GCCGCCTGGG ACGTGGAGTG GGCTGCACTT
    ACCTGCCGCC TGGAACAGGT GCCTGCCTGC GGGGTCTGTC CTGGGTCTCC CCAGCTCCTC
    CTCTGCCATC TGGGCCAGCT GGAAGAGAAG ACTGAGAAGA ATTTGGGAGA AGCATGCTCT
    TCCACCGACA GCAAAGGGGC ATTCAAGAAG GAAAGAGAGA TGCATCAGAA GCTCCTCACT
    GACAGTCATG GCCTGGTCAT GGACCTGCGC TGGCAGATCC ATCACAGTGA GAAGAACTGG
    AGCCGGGAGA AGGTGGAACT TCTTAGCCGT CTAGACAGAG ACCGGCAGGA ATGGGAGCAG
    CAGAAGAAGG AGCTCTTGTG GAGAATAGAA CAGTTGCAGA AAGAAAACAG TCCCCGGAGA
    AGTGGCAGTT TCCTCTGTGA TCAAAAAGAA GGCACCATCC GCCCCTTTGC CCATCCAGGA
    AGCCCCCGAA TGCCCCGTCC CTTGGGGATG TGGTCCTGTG CAGACACCGA CTCCACCCCA
    TTCGAAGACC GGCCGCTGTC CAAGCTGAAG GAATCAGACA GATGTTCAGC ACGGGAAAAC
    CTCTACTTGG ACGCCTTGTC TCTAGATGAC GAGCCAGAGG AGCCTCCAGC CCGAGAGCCC
    CAGCGGGAGT TCAGGAACTG CCTCCCCCAG GAAGAAGAAA ATCACAAGGG AAATCTTCAA
    AGGGCCGTGT CTGTGTCCTC CATGTCTGAG TTCCAGCGTC TGATGGACGT TTCTCCCTTC
    CTGCCGGAGA AGGGCGTCCC CGCCACCAGC AGCAAGGAGG ACATCACTCC GCCCCTGTCT
    CCTGATGATC TGAAGTACAT CGAGGAGTTC AACAGCAAGA ACTGGGACGA GAGGCCCCCG
    GAGCCCTGGG CAGACAGGAC CGAGGGGGGG CGGGCAGGGA GCGAGGCTGG TGCAGAGCCT
    TTCCCTGACT CCTCCTGGTA CCTCACCACG AGTGTCACCA TGACTACGGA CACCATGACC
    AGCCCAGAGC ACTGCCAGAA GCAGCCCCTT CGGAGCCCCA TGTGCGCGGA GCAGGCCGGG
    GTGCGGGTGC TGCACAGCCC GCCCGCGGTC CGTAGGATGG ATGGCATCGT GGTGGCGGGC
    AGTGAGGGCA AGGGCCGGGC CGAGCCCGAG TGCCCGTTTC CAACTAGCAG AGCCAGGGGG
    GGCACTGGAG ACACAAAAGG GGGTCCCTCA GAACACGTGC TCAGCAGGTG GCCTTGCGCC
    CCCTCCAGGC ACCCCCGAGA CTATGTGGAG GGAGGGCTGC GCCCCCTCGA AAGTGCCCTC
    TGCGCCCCCC TGGGTTTTGC CTCCTCGCTG CACGGCCTGC AGATGTCCAA GAACATGAGT
    GATGACATGA AGGAGGTGGC CTTCTCGGTC AGGAACGCCA TCTGCTCTGG CCCCCCCAAG
    CCTCCGCTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGACGGG AACACAGACC
    GTCCAGACCA TCACCATTGG CCTGCAGACC GAAGCCCTGC GTGGCAGCGC CGTCACCAGC
    AGCCCCCACA AGTGTCTCAC ACCCAAGGCC GGGGGCGGGG CCACGCCCGC GTCATCTCCT
    TCCCGTGGCC TTCGGAGCCG GCAGATGGCC CCCGCCATCG AGAAGGTGCA GGCCAAGTTC
    GAACGCACGT GCTGCTCCCC CAAGTACGGG TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC
    AAAGCTGAGC CGCCCAACAG CAGGACCTCC CTGGGGCTGG CCCCAAAGGG GTGCAGCGAG
    TCCGCCTGGG CCCGTTCCAC CACCACGAGG GAGAGCCCGG TGCACACCGC CATCAACGAT
    GGCCTCTCCA GCCTCTTCAA CATCATCGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG
    AAGGGGACTC GGGCCGGGAG CCGATCTCGC TCAGCAGAAC CCCGGCCCGA GCTGGGCCCA
    GGCCAGGAAA CGGGCCCCAG TTCCCGGGGC CGGTCCCCCA GCCCCATCGG GGCTGGCTCA
    GAGACTTGGA GGGAGGAAGG GGGAGAGGGC ACGCCCTTGA GGCAGGACTT ATCCGCTCCC
    CCTGGTTACA CGTTCACTGA GAACGTAGCC CGGATCCTCA ACAAGAAGCT GTTGGAGCAT
    GCCTTAAAGG AGGAGAGGAG GCAGGCCCCC CACGGGCCCC CAAGTCTTAA CAGTGACAGC
    CACATGGGAG AAACCATCAA AGCTGAACCA GGGTCCATCG AGGAACTACC TTGTTCCGCA
    CTAGCTCCAT CCCTAGAACC CTGCTTCTCC AGGCCCGAGA GACCAGCAAA CCGTCGCCTT
    CCGTCCCGTT GGGCCTCACA TTCCCCCACT GCCTCACTGG CTCAGTCACC CGGAGACCCA
    GCCTCCTTGG AGGAGCTCGG CGATGAGGAG CCACCGGGGG AGAAGACACA CCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe289890
    Clone ID Related Accession (Same CDS sequence) XM_022421631.1
    Accession Version XM_022421631.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product microtubule cross-linking factor 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG
    CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG
    TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG
    CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC
    ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG
    GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT
    CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG
    TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC
    CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG
    CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG
    TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC
    CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC
    CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG
    GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG
    GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA
    TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC
    GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG
    GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG
    GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG
    ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT
    GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC
    TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG
    CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG
    TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG
    CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG AGTGTATCGC TAGAGGAGCT GCAGGGTCAG
    CTTGCGCAGG CGACCAGACT GCATCAAGAG GAGACAGAGA AATTTACAAA CAAGATTCGG
    AAGATGGAGG AGGAGCACCT GTACGCCTTG AGGTGGAAAG AACTGGAAAT GCACAGCCTG
    GCTTTGCAGA ACACCCTCCA TGAGCAAACC TGGAGTGATG AGAGGAACCT GATGCAGCAG
    GAGCTCCGGT CTATGAAGCA GAACATTTTC CTTTTCTACG TCAAACTCAG ATGGCTGCTG
    AGGCACTGGC GACAAGGGAA GCAGATGGAG GAAGAAGGCG AGGATTTCAC TGAGAGCGAG
    CATCCAGAGA CCCTCCCCAG GCTTGGTGAG CTTGGAGTGC AGGGGGACCC GAAGGGGGAC
    GGCCCAGACC AAGATGACAA CGGTCCGGGC TGCGGCTTTC CGCTGGGAGA GCATCCTCCA
    CACTCCCCTG TGCAGATTGG TGACCATGGA TCGAGACTAC AGCCTACACA TGGGAGACAG
    CTCCACAGGC AGGTGGTGGA AAACCAGCAG CTGTTCAGTG CACTCAAGAG CCTGCTGGAG
    GACCTGCGCG CCGAGCTGCG GGAGGACGAG CATGCACGGC GGCGGCTGCA GCAGCAGTAC
    GCCAGCGACA AAGCCGCCTG GGACGTGGAG TGGGCTGCAC TTACCTGCCG CCTGGAACAG
    CTGGAAGAGA AGACTGAGAA GAATTTGGGA GAAGCATGCT CTTCCACCGA CAGCAAAGGG
    GCATTCAAGA AGGAAAGAGA GATGCATCAG AAGCTCCTCA CTGACAGTCA TGGCCTGGTC
    ATGGACCTGC GCTGGCAGAT CCATCACAGT GAGAAGAACT GGAGCCGGGA GAAGGTGGAA
    CTTCTTAGCC GTCTAGACAG AGACCGGCAG GAATGGGAGC AGCAGAAGAA GGAGCTCTTG
    TGGAGAATAG AACAGTTGCA GAAAGAAAAC AGTCCCCGGA GAAGTGGCAG TTTCCTCTGT
    GATCAAAAAG AAGGCACCAT CCGCCCCTTT GCCCATCCAG GAAGCCCCCG AATGCCCCGT
    CCCTTGGGGA TGTGGTCCTG TGCAGACACC GACTCCACCC CATTCGAAGA CCGGCCGCTG
    TCCAAGCTGA AGGAATCAGA CAGATGTTCA GCACGGGAAA ACCTCTACTT GGACGCCTTG
    TCTCTAGATG ACGAGCCAGA GGAGCCTCCA GCCCGAGAGC CCCAGCGGGA GTTCAGGAAC
    TGCCTCCCCC AGGAAGAAGA AAATCACAAG GGAAATCTTC AAAGGGCCGT GTCTGTGTCC
    TCCATGTCTG AGTTCCAGCG TCTGATGGAC GTTTCTCCCT TCCTGCCGGA GAAGGGCGTC
    CCCGCCACCA GCAGCAAGGA GGACATCACT CCGCCCCTGT CTCCTGATGA TCTGAAGTAC
    ATCGAGGAGT TCAACAGCAA GAACTGGGAC GAGAGGCCCC CGGAGCCCTG GGCAGACAGG
    ACCGAGGGGG GGCGGGCAGG GAGCGAGGCT GGTGCAGAGC CTTTCCCTGA CTCCTCCTGG
    TACCTCACCA CGAGTGTCAC CATGACTACG GACACCATGA CCAGCCCAGA GCACTGCCAG
    AAGCAGCCCC TTCGGAGCCC CATGTGCGCG GAGCAGGCCG GGGTGCGGGT GCTGCACAGC
    CCGCCCGCGG TCCGTAGGAT GGATGGCATC GTGGTGGCGG GCAGTGAGGG CAAGGGCCGG
    GCCGAGCCCG AGTGCCCGTT TCCAACTAGC AGAGCCAGGG GGGGCACTGG AGACACAAAA
    GGGGGTCCCT CAGAACACGT GCTCAGCAGG TGGCCTTGCG CCCCCTCCAG GCACCCCCGA
    GACTATGTGG AGGGAGGGCT GCGCCCCCTC GAAAGTGCCC TCTGCGCCCC CCTGGGTTTT
    GCCTCCTCGC TGCACGGCCT GCAGATGTCC AAGAACATGA GTGATGACAT GAAGGAGGTG
    GCCTTCTCGG TCAGGAACGC CATCTGCTCT GGCCCCCCCA AGCCTCCGCT CAAGGACATG
    GCCTGCCAGA CCAATGGGTC CCGGACGACG GGAACACAGA CCGTCCAGAC CATCACCATT
    GGCCTGCAGA CCGAAGCCCT GCGTGGCAGC GCCGTCACCA GCAGCCCCCA CAAGTGTCTC
    ACACCCAAGG CCGGGGGCGG GGCCACGCCC GCGTCATCTC CTTCCCGTGG CCTTCGGAGC
    CGGCAGATGG CCCCCGCCAT CGAGAAGGTG CAGGCCAAGT TCGAACGCAC GTGCTGCTCC
    CCCAAGTACG GGTCTCCCAA GCTGCAGAGG AAGCCCCTCC CCAAAGCTGA GCCGCCCAAC
    AGCAGGACCT CCCTGGGGCT GGCCCCAAAG GGGTGCAGCG AGTCCGCCTG GGCCCGTTCC
    ACCACCACGA GGGAGAGCCC GGTGCACACC GCCATCAACG ATGGCCTCTC CAGCCTCTTC
    AACATCATCG ACCACAGCCC CGTGGTGCAG GACCCCTTCC AGAAGGGGAC TCGGGCCGGG
    AGCCGATCTC GCTCAGCAGA ACCCCGGCCC GAGCTGGGCC CAGGCCAGGA AACGGGCCCC
    AGTTCCCGGG GCCGGTCCCC CAGCCCCATC GGGGCTGGCT CAGAGACTTG GAGGGAGGAA
    GGGGGAGAGG GCACGCCCTT GAGGCAGGAC TTATCCGCTC CCCCTGGTTA CACGTTCACT
    GAGAACGTAG CCCGGATCCT CAACAAGAAG CTGTTGGAGC ATGCCTTAAA GGAGGAGAGG
    AGGCAGGCCC CCCACGGGCC CCCAAGTCTT AACAGTGACA GCCACATGGG AGAAACCATC
    AAAGCTGAAC CAGGGTCCAT CGAGAACCAA ACTGTCTTAC TAACTGCCCC CTGGGAACTC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022277339.1
    CDS118..4920
    Translation

    Target ORF information:

    RefSeq Version XM_022421631.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022421631.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG
    CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG
    TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG
    CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC
    ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG
    GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT
    CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG
    TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC
    CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG
    CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG
    TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC
    CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC
    CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG
    GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG
    GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA
    TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC
    GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG
    GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG
    GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG
    ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT
    GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC
    TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG
    CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG
    TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG
    CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG AGTGTATCGC TAGAGGAGCT GCAGGGTCAG
    CTTGCGCAGG CGACCAGACT GCATCAAGAG GAGACAGAGA AATTTACAAA CAAGATTCGG
    AAGATGGAGG AGGAGCACCT GTACGCCTTG AGGTGGAAAG AACTGGAAAT GCACAGCCTG
    GCTTTGCAGA ACACCCTCCA TGAGCAAACC TGGAGTGATG AGAGGAACCT GATGCAGCAG
    GAGCTCCGGT CTATGAAGCA GAACATTTTC CTTTTCTACG TCAAACTCAG ATGGCTGCTG
    AGGCACTGGC GACAAGGGAA GCAGATGGAG GAAGAAGGCG AGGATTTCAC TGAGAGCGAG
    CATCCAGAGA CCCTCCCCAG GCTTGGTGAG CTTGGAGTGC AGGGGGACCC GAAGGGGGAC
    GGCCCAGACC AAGATGACAA CGGTCCGGGC TGCGGCTTTC CGCTGGGAGA GCATCCTCCA
    CACTCCCCTG TGCAGATTGG TGACCATGGA TCGAGACTAC AGCCTACACA TGGGAGACAG
    CTCCACAGGC AGGTGGTGGA AAACCAGCAG CTGTTCAGTG CACTCAAGAG CCTGCTGGAG
    GACCTGCGCG CCGAGCTGCG GGAGGACGAG CATGCACGGC GGCGGCTGCA GCAGCAGTAC
    GCCAGCGACA AAGCCGCCTG GGACGTGGAG TGGGCTGCAC TTACCTGCCG CCTGGAACAG
    CTGGAAGAGA AGACTGAGAA GAATTTGGGA GAAGCATGCT CTTCCACCGA CAGCAAAGGG
    GCATTCAAGA AGGAAAGAGA GATGCATCAG AAGCTCCTCA CTGACAGTCA TGGCCTGGTC
    ATGGACCTGC GCTGGCAGAT CCATCACAGT GAGAAGAACT GGAGCCGGGA GAAGGTGGAA
    CTTCTTAGCC GTCTAGACAG AGACCGGCAG GAATGGGAGC AGCAGAAGAA GGAGCTCTTG
    TGGAGAATAG AACAGTTGCA GAAAGAAAAC AGTCCCCGGA GAAGTGGCAG TTTCCTCTGT
    GATCAAAAAG AAGGCACCAT CCGCCCCTTT GCCCATCCAG GAAGCCCCCG AATGCCCCGT
    CCCTTGGGGA TGTGGTCCTG TGCAGACACC GACTCCACCC CATTCGAAGA CCGGCCGCTG
    TCCAAGCTGA AGGAATCAGA CAGATGTTCA GCACGGGAAA ACCTCTACTT GGACGCCTTG
    TCTCTAGATG ACGAGCCAGA GGAGCCTCCA GCCCGAGAGC CCCAGCGGGA GTTCAGGAAC
    TGCCTCCCCC AGGAAGAAGA AAATCACAAG GGAAATCTTC AAAGGGCCGT GTCTGTGTCC
    TCCATGTCTG AGTTCCAGCG TCTGATGGAC GTTTCTCCCT TCCTGCCGGA GAAGGGCGTC
    CCCGCCACCA GCAGCAAGGA GGACATCACT CCGCCCCTGT CTCCTGATGA TCTGAAGTAC
    ATCGAGGAGT TCAACAGCAA GAACTGGGAC GAGAGGCCCC CGGAGCCCTG GGCAGACAGG
    ACCGAGGGGG GGCGGGCAGG GAGCGAGGCT GGTGCAGAGC CTTTCCCTGA CTCCTCCTGG
    TACCTCACCA CGAGTGTCAC CATGACTACG GACACCATGA CCAGCCCAGA GCACTGCCAG
    AAGCAGCCCC TTCGGAGCCC CATGTGCGCG GAGCAGGCCG GGGTGCGGGT GCTGCACAGC
    CCGCCCGCGG TCCGTAGGAT GGATGGCATC GTGGTGGCGG GCAGTGAGGG CAAGGGCCGG
    GCCGAGCCCG AGTGCCCGTT TCCAACTAGC AGAGCCAGGG GGGGCACTGG AGACACAAAA
    GGGGGTCCCT CAGAACACGT GCTCAGCAGG TGGCCTTGCG CCCCCTCCAG GCACCCCCGA
    GACTATGTGG AGGGAGGGCT GCGCCCCCTC GAAAGTGCCC TCTGCGCCCC CCTGGGTTTT
    GCCTCCTCGC TGCACGGCCT GCAGATGTCC AAGAACATGA GTGATGACAT GAAGGAGGTG
    GCCTTCTCGG TCAGGAACGC CATCTGCTCT GGCCCCCCCA AGCCTCCGCT CAAGGACATG
    GCCTGCCAGA CCAATGGGTC CCGGACGACG GGAACACAGA CCGTCCAGAC CATCACCATT
    GGCCTGCAGA CCGAAGCCCT GCGTGGCAGC GCCGTCACCA GCAGCCCCCA CAAGTGTCTC
    ACACCCAAGG CCGGGGGCGG GGCCACGCCC GCGTCATCTC CTTCCCGTGG CCTTCGGAGC
    CGGCAGATGG CCCCCGCCAT CGAGAAGGTG CAGGCCAAGT TCGAACGCAC GTGCTGCTCC
    CCCAAGTACG GGTCTCCCAA GCTGCAGAGG AAGCCCCTCC CCAAAGCTGA GCCGCCCAAC
    AGCAGGACCT CCCTGGGGCT GGCCCCAAAG GGGTGCAGCG AGTCCGCCTG GGCCCGTTCC
    ACCACCACGA GGGAGAGCCC GGTGCACACC GCCATCAACG ATGGCCTCTC CAGCCTCTTC
    AACATCATCG ACCACAGCCC CGTGGTGCAG GACCCCTTCC AGAAGGGGAC TCGGGCCGGG
    AGCCGATCTC GCTCAGCAGA ACCCCGGCCC GAGCTGGGCC CAGGCCAGGA AACGGGCCCC
    AGTTCCCGGG GCCGGTCCCC CAGCCCCATC GGGGCTGGCT CAGAGACTTG GAGGGAGGAA
    GGGGGAGAGG GCACGCCCTT GAGGCAGGAC TTATCCGCTC CCCCTGGTTA CACGTTCACT
    GAGAACGTAG CCCGGATCCT CAACAAGAAG CTGTTGGAGC ATGCCTTAAA GGAGGAGAGG
    AGGCAGGCCC CCCACGGGCC CCCAAGTCTT AACAGTGACA GCCACATGGG AGAAACCATC
    AAAGCTGAAC CAGGGTCCAT CGAGAACCAA ACTGTCTTAC TAACTGCCCC CTGGGAACTC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe09960
    Clone ID Related Accession (Same CDS sequence) XM_005623075.1
    Accession Version XM_005623075.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4989bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product microtubule cross-linking factor 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726068.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG
    CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG
    TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG
    CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC
    ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG
    GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT
    CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG
    TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC
    CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG
    CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG
    TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC
    CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC
    CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG
    GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG
    GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA
    TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC
    GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG
    GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG
    GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG
    ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT
    GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC
    TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG
    CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG
    TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG
    CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG CTCAGGGGCC CCCCTGTTTT ACCTGAACAG
    AGTGTATCGC TAGAGGAGCT GCAGGGTCAG CTTGCGCAGG CGACCAGACT GCATCAAGAG
    GAGACAGAGA AATTTACAAA CAAGATTCGG AAGATGGAGG AGGAGCACCT GTACGCCTTG
    AGGTGGAAAG AACTGGAAAT GCACAGCCTG GCTTTGCAGA ACACCCTCCA TGAGCAAACC
    TGGAGTGATG AGAGGAACCT GATGCAGCAG GAGCTCCGGT CTATGAAGCA GAACATTTTC
    CTTTTCTACG TCAAACTCAG ATGGCTGCTG AGGCACTGGC GACAAGGGAA GCAGATGGAG
    GAAGAAGGCG AGGATTTCAC TGAGAGCGAG CATCCAGAGA CCCTCCCCAG GCTTGGTGAG
    CTTGGAGTGC AGGGGGACCC GAAGGGGGAC GGCCCAGACC AAGATGACAA CGGTCCGGGC
    TGCGGCTTTC CGCTGGGAGA GCATCCTCCA CACTCCCCTG TGCAGATTGG TGACCATGGA
    TCGAGACTAC AGCCTACACA TGGGAGACAG CTCCACAGGC AGGTGGTGGA AAACCAGCAG
    CTGTTCAGTG CACTCAAGAG CCTGCTGGAG GACCTGCGCG CCGAGCTGCG GGAGGACGAG
    CATGCACGGC GGCGGCTGCA GCAGCAGTAC GCCAGCGACA AAGCCGCCTG GGACGTGGAG
    TGGGCTGCAC TTACCTGCCG CCTGGAACAG CTGGAAGAGA AGACTGAGAA GAATTTGGGA
    GAAGCATGCT CTTCCACCGA CAGCAAAGGG GCATTCAAGA AGGAAAGAGA GATGCATCAG
    AAGCTCCTCA CTGACAGTCA TGGCCTGGTC ATGGACCTGC GCTGGCAGAT CCATCACAGT
    GAGAAGAACT GGAGCCGGGA GAAGGTGGAA CTTCTTAGCC GTCTAGACAG AGACCGGCAG
    GAATGGGAGC AGCAGAAGAA GGAGCTCTTG TGGAGAATAG AACAGTTGCA GAAAGAAAAC
    AGTCCCCGGA GAAGTGGCAG TTTCCTCTGT GATCAAAAAG AAGGCACCAT CCGCCCCTTT
    GCCCATCCAG GAAGCCCCCG AATGCCCCGT CCCTTGGGGA TGTGGTCCTG TGCAGACACC
    GACTCCACCC CATTCGAAGA CCGGCCGCTG TCCAAGCTGA AGGAATCAGA CAGATGTTCA
    GCACGGGAAA ACCTCTACTT GGACGCCTTG TCTCTAGATG ACGAGCCAGA GGAGCCTCCA
    GCCCGAGAGC CCCAGCGGGA GTTCAGGAAC TGCCTCCCCC AGGAAGAAGA AAATCACAAG
    GGAAATCTTC AAAGGGCCGT GTCTGTGTCC TCCATGTCTG AGTTCCAGCG TCTGATGGAC
    GTTTCTCCCT TCCTGCCGGA GAAGGGCGTC CCCGCCACCA GCAGCAAGGA GGACATCACT
    CCGCCCCTGT CTCCTGATGA TCTGAAGTAC ATCGAGGAGT TCAACAGCAA GAACTGGGAC
    GAGAGGCCCC CGGAGCCCTG GGCAGACAGG ACCGAGGGGG GGCGGGCAGG GAGCGAGGCT
    GGTGCAGAGC CTTTCCCTGA CTCCTCCTGG TACCTCACCA CGAGTGTCAC CATGACTACG
    GACACCATGA CCAGCCCAGA GCACTGCCAG AAGCAGCCCC TTCGGAGCCC CATGTGCGCG
    GAGCAGGCCG GGGTGCGGGT GCTGCACAGC CCGCCCGCGG TCCGTAGGAT GGATGGCATC
    GTGGTGGCGG GCAGTGAGGG CAAGGGCCGG GCCGAGCCCG AGTGCCCGTT TCCAACTAGC
    AGAGCCAGGG GGGGCACTGG AGACACAAAA GGGGGTCCCT CAGAACACGT GCTCAGCAGG
    TGGCCTTGCG CCCCCTCCAG GCACCCCCGA GACTATGTGG AGGGAGGGCT GCGCCCCCTC
    GAAAGTGCCC TCTGCGCCCC CCTGGGTTTT GCCTCCTCGC TGCACGGCCT GCAGATGTCC
    AAGAACATGA GTGATGACAT GAAGGAGGTG GCCTTCTCGG TCAGGAACGC CATCTGCTCT
    GGCCCCCCCA AGCCTCCGCT CAAGGACATG GCCTGCCAGA CCAATGGGTC CCGGACGACG
    GGAACACAGA CCGTCCAGAC CATCACCATT GGCCTGCAGA CCGAAGCCCT GCGTGGCAGC
    GCCGTCACCA GCAGCCCCCA CAAGTGTCTC ACACCCAAGG CCGGGGGCGG GGCCACGCCC
    GCGTCATCTC CTTCCCGTGG CCTTCGGAGC CGGCAGATGG CCCCCGCCAT CGAGAAGGTG
    CAGGCCAAGT TCGAACGCAC GTGCTGCTCC CCCAAGTACG GGTCTCCCAA GCTGCAGAGG
    AAGCCCCTCC CCAAAGCTGA GCCGCCCAAC AGCAGGACCT CCCTGGGGCT GGCCCCAAAG
    GGGTGCAGCG AGTCCGCCTG GGCCCGTTCC ACCACCACGA GGGAGAGCCC GGTGCACACC
    GCCATCAACG ATGGCCTCTC CAGCCTCTTC AACATCATCG ACCACAGCCC CGTGGTGCAG
    GACCCCTTCC AGAAGGGGAC TCGGGCCGGG AGCCGATCTC GCTCAGCAGA ACCCCGGCCC
    GAGCTGGGCC CAGGCCAGGA AACGGGCCCC AGTTCCCGGG GCCGGTCCCC CAGCCCCATC
    GGGGCTGGCT CAGAGACTTG GAGGGAGGAA GGGGGAGAGG GCACGCCCTT GAGGCAGGAC
    TTATCCGCTC CCCCTGGTTA CACGTTCACT GAGAACGTAG CCCGGATCCT CAACAAGAAG
    CTGTTGGAGC ATGCCTTAAA GGAGGAGAGG AGGCAGGCCC CCCACGGGCC CCCAAGTCTT
    AACAGTGACA GCCACATGGG AGAAACCATC AAAGCTGAAC CAGGGTCCAT CGAGGAACTA
    CCTTGTTCCG CACTAGCTCC ATCCCTAGAA CCCTGCTTCT CCAGGCCCGA GAGACCAGCA
    AACCGTCGCC TTCCGTCCCG TTGGGCCTCA CATTCCCCCA CTGCCTCACT GGCTCAGTCA
    CCCGGAGACC CAGCCTCCTT GGAGGAGCTC GGCGATGAGG AGCCACCGGG GGAGAAGACA
    CACCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005623132.1
    CDS80..5068
    Translation

    Target ORF information:

    RefSeq Version XM_005623075.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005623075.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG
    CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG
    TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG
    CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC
    ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG
    GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT
    CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG
    TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC
    CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG
    CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG
    TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC
    CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC
    CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG
    GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG
    GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA
    TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC
    GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG
    GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG
    GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG
    ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT
    GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC
    TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG
    CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG
    TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG
    CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG CTCAGGGGCC CCCCTGTTTT ACCTGAACAG
    AGTGTATCGC TAGAGGAGCT GCAGGGTCAG CTTGCGCAGG CGACCAGACT GCATCAAGAG
    GAGACAGAGA AATTTACAAA CAAGATTCGG AAGATGGAGG AGGAGCACCT GTACGCCTTG
    AGGTGGAAAG AACTGGAAAT GCACAGCCTG GCTTTGCAGA ACACCCTCCA TGAGCAAACC
    TGGAGTGATG AGAGGAACCT GATGCAGCAG GAGCTCCGGT CTATGAAGCA GAACATTTTC
    CTTTTCTACG TCAAACTCAG ATGGCTGCTG AGGCACTGGC GACAAGGGAA GCAGATGGAG
    GAAGAAGGCG AGGATTTCAC TGAGAGCGAG CATCCAGAGA CCCTCCCCAG GCTTGGTGAG
    CTTGGAGTGC AGGGGGACCC GAAGGGGGAC GGCCCAGACC AAGATGACAA CGGTCCGGGC
    TGCGGCTTTC CGCTGGGAGA GCATCCTCCA CACTCCCCTG TGCAGATTGG TGACCATGGA
    TCGAGACTAC AGCCTACACA TGGGAGACAG CTCCACAGGC AGGTGGTGGA AAACCAGCAG
    CTGTTCAGTG CACTCAAGAG CCTGCTGGAG GACCTGCGCG CCGAGCTGCG GGAGGACGAG
    CATGCACGGC GGCGGCTGCA GCAGCAGTAC GCCAGCGACA AAGCCGCCTG GGACGTGGAG
    TGGGCTGCAC TTACCTGCCG CCTGGAACAG CTGGAAGAGA AGACTGAGAA GAATTTGGGA
    GAAGCATGCT CTTCCACCGA CAGCAAAGGG GCATTCAAGA AGGAAAGAGA GATGCATCAG
    AAGCTCCTCA CTGACAGTCA TGGCCTGGTC ATGGACCTGC GCTGGCAGAT CCATCACAGT
    GAGAAGAACT GGAGCCGGGA GAAGGTGGAA CTTCTTAGCC GTCTAGACAG AGACCGGCAG
    GAATGGGAGC AGCAGAAGAA GGAGCTCTTG TGGAGAATAG AACAGTTGCA GAAAGAAAAC
    AGTCCCCGGA GAAGTGGCAG TTTCCTCTGT GATCAAAAAG AAGGCACCAT CCGCCCCTTT
    GCCCATCCAG GAAGCCCCCG AATGCCCCGT CCCTTGGGGA TGTGGTCCTG TGCAGACACC
    GACTCCACCC CATTCGAAGA CCGGCCGCTG TCCAAGCTGA AGGAATCAGA CAGATGTTCA
    GCACGGGAAA ACCTCTACTT GGACGCCTTG TCTCTAGATG ACGAGCCAGA GGAGCCTCCA
    GCCCGAGAGC CCCAGCGGGA GTTCAGGAAC TGCCTCCCCC AGGAAGAAGA AAATCACAAG
    GGAAATCTTC AAAGGGCCGT GTCTGTGTCC TCCATGTCTG AGTTCCAGCG TCTGATGGAC
    GTTTCTCCCT TCCTGCCGGA GAAGGGCGTC CCCGCCACCA GCAGCAAGGA GGACATCACT
    CCGCCCCTGT CTCCTGATGA TCTGAAGTAC ATCGAGGAGT TCAACAGCAA GAACTGGGAC
    GAGAGGCCCC CGGAGCCCTG GGCAGACAGG ACCGAGGGGG GGCGGGCAGG GAGCGAGGCT
    GGTGCAGAGC CTTTCCCTGA CTCCTCCTGG TACCTCACCA CGAGTGTCAC CATGACTACG
    GACACCATGA CCAGCCCAGA GCACTGCCAG AAGCAGCCCC TTCGGAGCCC CATGTGCGCG
    GAGCAGGCCG GGGTGCGGGT GCTGCACAGC CCGCCCGCGG TCCGTAGGAT GGATGGCATC
    GTGGTGGCGG GCAGTGAGGG CAAGGGCCGG GCCGAGCCCG AGTGCCCGTT TCCAACTAGC
    AGAGCCAGGG GGGGCACTGG AGACACAAAA GGGGGTCCCT CAGAACACGT GCTCAGCAGG
    TGGCCTTGCG CCCCCTCCAG GCACCCCCGA GACTATGTGG AGGGAGGGCT GCGCCCCCTC
    GAAAGTGCCC TCTGCGCCCC CCTGGGTTTT GCCTCCTCGC TGCACGGCCT GCAGATGTCC
    AAGAACATGA GTGATGACAT GAAGGAGGTG GCCTTCTCGG TCAGGAACGC CATCTGCTCT
    GGCCCCCCCA AGCCTCCGCT CAAGGACATG GCCTGCCAGA CCAATGGGTC CCGGACGACG
    GGAACACAGA CCGTCCAGAC CATCACCATT GGCCTGCAGA CCGAAGCCCT GCGTGGCAGC
    GCCGTCACCA GCAGCCCCCA CAAGTGTCTC ACACCCAAGG CCGGGGGCGG GGCCACGCCC
    GCGTCATCTC CTTCCCGTGG CCTTCGGAGC CGGCAGATGG CCCCCGCCAT CGAGAAGGTG
    CAGGCCAAGT TCGAACGCAC GTGCTGCTCC CCCAAGTACG GGTCTCCCAA GCTGCAGAGG
    AAGCCCCTCC CCAAAGCTGA GCCGCCCAAC AGCAGGACCT CCCTGGGGCT GGCCCCAAAG
    GGGTGCAGCG AGTCCGCCTG GGCCCGTTCC ACCACCACGA GGGAGAGCCC GGTGCACACC
    GCCATCAACG ATGGCCTCTC CAGCCTCTTC AACATCATCG ACCACAGCCC CGTGGTGCAG
    GACCCCTTCC AGAAGGGGAC TCGGGCCGGG AGCCGATCTC GCTCAGCAGA ACCCCGGCCC
    GAGCTGGGCC CAGGCCAGGA AACGGGCCCC AGTTCCCGGG GCCGGTCCCC CAGCCCCATC
    GGGGCTGGCT CAGAGACTTG GAGGGAGGAA GGGGGAGAGG GCACGCCCTT GAGGCAGGAC
    TTATCCGCTC CCCCTGGTTA CACGTTCACT GAGAACGTAG CCCGGATCCT CAACAAGAAG
    CTGTTGGAGC ATGCCTTAAA GGAGGAGAGG AGGCAGGCCC CCCACGGGCC CCCAAGTCTT
    AACAGTGACA GCCACATGGG AGAAACCATC AAAGCTGAAC CAGGGTCCAT CGAGGAACTA
    CCTTGTTCCG CACTAGCTCC ATCCCTAGAA CCCTGCTTCT CCAGGCCCGA GAGACCAGCA
    AACCGTCGCC TTCCGTCCCG TTGGGCCTCA CATTCCCCCA CTGCCTCACT GGCTCAGTCA
    CCCGGAGACC CAGCCTCCTT GGAGGAGCTC GGCGATGAGG AGCCACCGGG GGAGAAGACA
    CACCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe09963
    Clone ID Related Accession (Same CDS sequence) XM_005623078.1
    Accession Version XM_005623078.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product microtubule cross-linking factor 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726068.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG
    CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG
    TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG
    CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC
    ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG
    GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT
    CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG
    TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC
    CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG
    CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG
    TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC
    CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC
    CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG
    GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG
    GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA
    TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC
    GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG
    GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG
    GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG
    ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT
    GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC
    TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG
    CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG
    TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG
    CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG CTCAGGGGCC CCCCTGTTTT ACCTGAACAG
    AGTGTATCGC TAGAGGAGCT GCAGGGTCAG CTTGCGCAGG CGACCAGACT GCATCAAGAG
    GAGACAGAGA AATTTACAAA CAAGATTCGG AAGATGGAGG AGGAGCACCT GTACGCCTTG
    AGGTGGAAAG AACTGGAAAT GCACAGCCTG GCTTTGCAGA ACACCCTCCA TGAGCAAACC
    TGGAGTGATG AGAGGAACCT GATGCAGCAG GAGCTCCGGT CTATGAAGCA GAACATTTTC
    CTTTTCTACG TCAAACTCAG ATGGCTGCTG AGGCACTGGC GACAAGGGAA GCAGATGGAG
    GAAGAAGGCG AGGATTTCAC TGAGAGCGAG CATCCAGAGA CCCTCCCCAG GCTTGGTGAG
    CTTGGAGTGC AGGGGGACCC GAAGGGGGAC GGCCCAGACC AAGATGACAA CGGTCCGGGC
    TGCGGCTTTC CGCTGGGAGA GCATCCTCCA CACTCCCCTG TGCAGATTGG TGACCATGGA
    TCGAGACTAC AGCCTACACA TGGGAGACAG CTCCACAGGC AGGTGGTGGA AAACCAGCAG
    CTGTTCAGTG CACTCAAGAG CCTGCTGGAG GACCTGCGCG CCGAGCTGCG GGAGGACGAG
    CATGCACGGC GGCGGCTGCA GCAGCAGTAC GCCAGCGACA AAGCCGCCTG GGACGTGGAG
    TGGGCTGCAC TTACCTGCCG CCTGGAACAG CTGGAAGAGA AGACTGAGAA GAATTTGGGA
    GAAGCATGCT CTTCCACCGA CAGCAAAGGG GCATTCAAGA AGGAAAGAGA GATGCATCAG
    AAGCTCCTCA CTGACAGTCA TGGCCTGGTC ATGGACCTGC GCTGGCAGAT CCATCACAGT
    GAGAAGAACT GGAGCCGGGA GAAGGTGGAA CTTCTTAGCC GTCTAGACAG AGACCGGCAG
    GAATGGGAGC AGCAGAAGAA GGAGCTCTTG TGGAGAATAG AACAGTTGCA GAAAGAAAAC
    AGTCCCCGGA GAAGTGGCAG TTTCCTCTGT GATCAAAAAG AAGGCACCAT CCGCCCCTTT
    GCCCATCCAG GAAGCCCCCG AATGCCCCGT CCCTTGGGGA TGTGGTCCTG TGCAGACACC
    GACTCCACCC CATTCGAAGA CCGGCCGCTG TCCAAGCTGA AGGAATCAGA CAGATGTTCA
    GCACGGGAAA ACCTCTACTT GGACGCCTTG TCTCTAGATG ACGAGCCAGA GGAGCCTCCA
    GCCCGAGAGC CCCAGCGGGA GTTCAGGAAC TGCCTCCCCC AGGAAGAAGA AAATCACAAG
    GGAAATCTTC AAAGGGCCGT GTCTGTGTCC TCCATGTCTG AGTTCCAGCG TCTGATGGAC
    GTTTCTCCCT TCCTGCCGGA GAAGGGCGTC CCCGCCACCA GCAGCAAGGA GGACATCACT
    CCGCCCCTGT CTCCTGATGA TCTGAAGTAC ATCGAGGAGT TCAACAGCAA GAACTGGGAC
    GAGAGGCCCC CGGAGCCCTG GGCAGACAGG ACCGAGGGGG GGCGGGCAGG GAGCGAGGCT
    GGTGCAGAGC CTTTCCCTGA CTCCTCCTGG TACCTCACCA CGAGTGTCAC CATGACTACG
    GACACCATGA CCAGCCCAGA GCACTGCCAG AAGCAGCCCC TTCGGAGCCC CATGTGCGCG
    GAGCAGGCCG GGGTGCGGGT GCTGCACAGC CCGCCCGCGG TCCGTAGGAT GGATGGCATC
    GTGGTGGCGG GCAGTGAGGG CAAGGGCCGG GCCGAGCCCG AGTGCCCGTT TCCAACTAGC
    AGAGCCAGGG GGGGCACTGG AGACACAAAA GGGGGTCCCT CAGAACACGT GCTCAGCAGG
    TGGCCTTGCG CCCCCTCCAG GCACCCCCGA GACTATGTGG AGGGAGGGCT GCGCCCCCTC
    GAAAGTGCCC TCTGCGCCCC CCTGGGTTTT GCCTCCTCGC TGCACGGCCT GCAGATGTCC
    AAGAACATGA GTGATGACAT GAAGGAGGTG GCCTTCTCGG TCAGGAACGC CATCTGCTCT
    GGCCCCCCCA AGCCTCCGCT CAAGGACATG GCCTGCCAGA CCAATGGGTC CCGGACGACG
    GGAACACAGA CCGTCCAGAC CATCACCATT GGCCTGCAGA CCGAAGCCCT GCGTGGCAGC
    GCCGTCACCA GCAGCCCCCA CAAGTGTCTC ACACCCAAGG CCGGGGGCGG GGCCACGCCC
    GCGTCATCTC CTTCCCGTGG CCTTCGGAGC CGGCAGATGG CCCCCGCCAT CGAGAAGGTG
    CAGGCCAAGT TCGAACGCAC GTGCTGCTCC CCCAAGTACG GGTCTCCCAA GCTGCAGAGG
    AAGCCCCTCC CCAAAGCTGA GCCGCCCAAC AGCAGGACCT CCCTGGGGCT GGCCCCAAAG
    GGGTGCAGCG AGTCCGCCTG GGCCCGTTCC ACCACCACGA GGGAGAGCCC GGTGCACACC
    GCCATCAACG ATGGCCTCTC CAGCCTCTTC AACATCATCG ACCACAGCCC CGTGGTGCAG
    GACCCCTTCC AGAAGGGGAC TCGGGCCGGG AGCCGATCTC GCTCAGCAGA ACCCCGGCCC
    GAGCTGGGCC CAGGCCAGGA AACGGGCCCC AGTTCCCGGG GCCGGTCCCC CAGCCCCATC
    GGGGCTGGCT CAGAGACTTG GAGGGAGGAA GGGGGAGAGG GCACGCCCTT GAGGCAGGAC
    TTATCCGCTC CCCCTGGTTA CACGTTCACT GAGAACGTAG CCCGGATCCT CAACAAGAAG
    CTGTTGGAGC ATGCCTTAAA GGAGGAGAGG AGGCAGGCCC CCCACGGGCC CCCAAGTCTT
    AACAGTGACA GCCACATGGG AGAAACCATC AAAGCTGAAC CAGGGTCCAT CGAGAACCAA
    ACTGTCTTAC TAACTGCCCC CTGGGAACTC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005623135.1
    CDS80..4912
    Translation

    Target ORF information:

    RefSeq Version XM_005623078.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005623078.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG
    CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG
    TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG
    CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC
    ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG
    GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT
    CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG
    TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC
    CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG
    CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG
    TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC
    CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC
    CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG
    GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG
    GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA
    TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC
    GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG
    GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG
    GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG
    ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT
    GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC
    TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG
    CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG
    TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG
    CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG CTCAGGGGCC CCCCTGTTTT ACCTGAACAG
    AGTGTATCGC TAGAGGAGCT GCAGGGTCAG CTTGCGCAGG CGACCAGACT GCATCAAGAG
    GAGACAGAGA AATTTACAAA CAAGATTCGG AAGATGGAGG AGGAGCACCT GTACGCCTTG
    AGGTGGAAAG AACTGGAAAT GCACAGCCTG GCTTTGCAGA ACACCCTCCA TGAGCAAACC
    TGGAGTGATG AGAGGAACCT GATGCAGCAG GAGCTCCGGT CTATGAAGCA GAACATTTTC
    CTTTTCTACG TCAAACTCAG ATGGCTGCTG AGGCACTGGC GACAAGGGAA GCAGATGGAG
    GAAGAAGGCG AGGATTTCAC TGAGAGCGAG CATCCAGAGA CCCTCCCCAG GCTTGGTGAG
    CTTGGAGTGC AGGGGGACCC GAAGGGGGAC GGCCCAGACC AAGATGACAA CGGTCCGGGC
    TGCGGCTTTC CGCTGGGAGA GCATCCTCCA CACTCCCCTG TGCAGATTGG TGACCATGGA
    TCGAGACTAC AGCCTACACA TGGGAGACAG CTCCACAGGC AGGTGGTGGA AAACCAGCAG
    CTGTTCAGTG CACTCAAGAG CCTGCTGGAG GACCTGCGCG CCGAGCTGCG GGAGGACGAG
    CATGCACGGC GGCGGCTGCA GCAGCAGTAC GCCAGCGACA AAGCCGCCTG GGACGTGGAG
    TGGGCTGCAC TTACCTGCCG CCTGGAACAG CTGGAAGAGA AGACTGAGAA GAATTTGGGA
    GAAGCATGCT CTTCCACCGA CAGCAAAGGG GCATTCAAGA AGGAAAGAGA GATGCATCAG
    AAGCTCCTCA CTGACAGTCA TGGCCTGGTC ATGGACCTGC GCTGGCAGAT CCATCACAGT
    GAGAAGAACT GGAGCCGGGA GAAGGTGGAA CTTCTTAGCC GTCTAGACAG AGACCGGCAG
    GAATGGGAGC AGCAGAAGAA GGAGCTCTTG TGGAGAATAG AACAGTTGCA GAAAGAAAAC
    AGTCCCCGGA GAAGTGGCAG TTTCCTCTGT GATCAAAAAG AAGGCACCAT CCGCCCCTTT
    GCCCATCCAG GAAGCCCCCG AATGCCCCGT CCCTTGGGGA TGTGGTCCTG TGCAGACACC
    GACTCCACCC CATTCGAAGA CCGGCCGCTG TCCAAGCTGA AGGAATCAGA CAGATGTTCA
    GCACGGGAAA ACCTCTACTT GGACGCCTTG TCTCTAGATG ACGAGCCAGA GGAGCCTCCA
    GCCCGAGAGC CCCAGCGGGA GTTCAGGAAC TGCCTCCCCC AGGAAGAAGA AAATCACAAG
    GGAAATCTTC AAAGGGCCGT GTCTGTGTCC TCCATGTCTG AGTTCCAGCG TCTGATGGAC
    GTTTCTCCCT TCCTGCCGGA GAAGGGCGTC CCCGCCACCA GCAGCAAGGA GGACATCACT
    CCGCCCCTGT CTCCTGATGA TCTGAAGTAC ATCGAGGAGT TCAACAGCAA GAACTGGGAC
    GAGAGGCCCC CGGAGCCCTG GGCAGACAGG ACCGAGGGGG GGCGGGCAGG GAGCGAGGCT
    GGTGCAGAGC CTTTCCCTGA CTCCTCCTGG TACCTCACCA CGAGTGTCAC CATGACTACG
    GACACCATGA CCAGCCCAGA GCACTGCCAG AAGCAGCCCC TTCGGAGCCC CATGTGCGCG
    GAGCAGGCCG GGGTGCGGGT GCTGCACAGC CCGCCCGCGG TCCGTAGGAT GGATGGCATC
    GTGGTGGCGG GCAGTGAGGG CAAGGGCCGG GCCGAGCCCG AGTGCCCGTT TCCAACTAGC
    AGAGCCAGGG GGGGCACTGG AGACACAAAA GGGGGTCCCT CAGAACACGT GCTCAGCAGG
    TGGCCTTGCG CCCCCTCCAG GCACCCCCGA GACTATGTGG AGGGAGGGCT GCGCCCCCTC
    GAAAGTGCCC TCTGCGCCCC CCTGGGTTTT GCCTCCTCGC TGCACGGCCT GCAGATGTCC
    AAGAACATGA GTGATGACAT GAAGGAGGTG GCCTTCTCGG TCAGGAACGC CATCTGCTCT
    GGCCCCCCCA AGCCTCCGCT CAAGGACATG GCCTGCCAGA CCAATGGGTC CCGGACGACG
    GGAACACAGA CCGTCCAGAC CATCACCATT GGCCTGCAGA CCGAAGCCCT GCGTGGCAGC
    GCCGTCACCA GCAGCCCCCA CAAGTGTCTC ACACCCAAGG CCGGGGGCGG GGCCACGCCC
    GCGTCATCTC CTTCCCGTGG CCTTCGGAGC CGGCAGATGG CCCCCGCCAT CGAGAAGGTG
    CAGGCCAAGT TCGAACGCAC GTGCTGCTCC CCCAAGTACG GGTCTCCCAA GCTGCAGAGG
    AAGCCCCTCC CCAAAGCTGA GCCGCCCAAC AGCAGGACCT CCCTGGGGCT GGCCCCAAAG
    GGGTGCAGCG AGTCCGCCTG GGCCCGTTCC ACCACCACGA GGGAGAGCCC GGTGCACACC
    GCCATCAACG ATGGCCTCTC CAGCCTCTTC AACATCATCG ACCACAGCCC CGTGGTGCAG
    GACCCCTTCC AGAAGGGGAC TCGGGCCGGG AGCCGATCTC GCTCAGCAGA ACCCCGGCCC
    GAGCTGGGCC CAGGCCAGGA AACGGGCCCC AGTTCCCGGG GCCGGTCCCC CAGCCCCATC
    GGGGCTGGCT CAGAGACTTG GAGGGAGGAA GGGGGAGAGG GCACGCCCTT GAGGCAGGAC
    TTATCCGCTC CCCCTGGTTA CACGTTCACT GAGAACGTAG CCCGGATCCT CAACAAGAAG
    CTGTTGGAGC ATGCCTTAAA GGAGGAGAGG AGGCAGGCCC CCCACGGGCC CCCAAGTCTT
    AACAGTGACA GCCACATGGG AGAAACCATC AAAGCTGAAC CAGGGTCCAT CGAGAACCAA
    ACTGTCTTAC TAACTGCCCC CTGGGAACTC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe09965
    Clone ID Related Accession (Same CDS sequence) XM_005623080.2 , XM_005623080.3
    Accession Version XM_005623080.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product microtubule cross-linking factor 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726068.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005623080.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG
    CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG
    TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG
    CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC
    ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG
    GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT
    CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG
    TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC
    CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG
    CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG
    TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC
    CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC
    CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG
    GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG
    GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA
    TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC
    GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG
    GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG
    GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG
    ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT
    GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC
    TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG
    CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG
    TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG
    CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG ATGGAGGAGG AGCACCTGTA CGCCTTGAGG
    TGGAAAGAAC TGGAAATGCA CAGCCTGGCT TTGCAGAACA CCCTCCATGA GCAAACCTGG
    AGTGATGAGA GGAACCTGAT GCAGCAGGAG CTCCGGTCTA TGAAGCAGAA CATTTTCCTT
    TTCTACGTCA AACTCAGATG GCTGCTGAGG CACTGGCGAC AAGGGAAGCA GATGGAGGAA
    GAAGGCGAGG ATTTCACTGA GAGCGAGCAT CCAGAGACCC TCCCCAGGCT TGGTGAGCTT
    GGAGTGCAGG GGGACCCGAA GGGGGACGGC CCAGACCAAG ATGACAACGG TCCGGGCTGC
    GGCTTTCCGC TGGGAGAGCA TCCTCCACAC TCCCCTGTGC AGATTGGTGA CCATGGATCG
    AGACTACAGC CTACACATGG GAGACAGCTC CACAGGCAGG TGGTGGAAAA CCAGCAGCTG
    TTCAGTGCAC TCAAGAGCCT GCTGGAGGAC CTGCGCGCCG AGCTGCGGGA GGACGAGCAT
    GCACGGCGGC GGCTGCAGCA GCAGTACGCC AGCGACAAAG CCGCCTGGGA CGTGGAGTGG
    GCTGCACTTA CCTGCCGCCT GGAACAGCTG GAAGAGAAGA CTGAGAAGAA TTTGGGAGAA
    GCATGCTCTT CCACCGACAG CAAAGGGGCA TTCAAGAAGG AAAGAGAGAT GCATCAGAAG
    CTCCTCACTG ACAGTCATGG CCTGGTCATG GACCTGCGCT GGCAGATCCA TCACAGTGAG
    AAGAACTGGA GCCGGGAGAA GGTGGAACTT CTTAGCCGTC TAGACAGAGA CCGGCAGGAA
    TGGGAGCAGC AGAAGAAGGA GCTCTTGTGG AGAATAGAAC AGTTGCAGAA AGAAAACAGT
    CCCCGGAGAA GTGGCAGTTT CCTCTGTGAT CAAAAAGAAG GCACCATCCG CCCCTTTGCC
    CATCCAGGAA GCCCCCGAAT GCCCCGTCCC TTGGGGATGT GGTCCTGTGC AGACACCGAC
    TCCACCCCAT TCGAAGACCG GCCGCTGTCC AAGCTGAAGG AATCAGACAG ATGTTCAGCA
    CGGGAAAACC TCTACTTGGA CGCCTTGTCT CTAGATGACG AGCCAGAGGA GCCTCCAGCC
    CGAGAGCCCC AGCGGGAGTT CAGGAACTGC CTCCCCCAGG AAGAAGAAAA TCACAAGGGA
    AATCTTCAAA GGGCCGTGTC TGTGTCCTCC ATGTCTGAGT TCCAGCGTCT GATGGACGTT
    TCTCCCTTCC TGCCGGAGAA GGGCGTCCCC GCCACCAGCA GCAAGGAGGA CATCACTCCG
    CCCCTGTCTC CTGATGATCT GAAGTACATC GAGGAGTTCA ACAGCAAGAA CTGGGACGAG
    AGGCCCCCGG AGCCCTGGGC AGACAGGACC GAGGGGGGGC GGGCAGGGAG CGAGGCTGGT
    GCAGAGCCTT TCCCTGACTC CTCCTGGTAC CTCACCACGA GTGTCACCAT GACTACGGAC
    ACCATGACCA GCCCAGAGCA CTGCCAGAAG CAGCCCCTTC GGAGCCCCAT GTGCGCGGAG
    CAGGCCGGGG TGCGGGTGCT GCACAGCCCG CCCGCGGTCC GTAGGATGGA TGGCATCGTG
    GTGGCGGGCA GTGAGGGCAA GGGCCGGGCC GAGCCCGAGT GCCCGTTTCC AACTAGCAGA
    GCCAGGGGGG GCACTGGAGA CACAAAAGGG GGTCCCTCAG AACACGTGCT CAGCAGGTGG
    CCTTGCGCCC CCTCCAGGCA CCCCCGAGAC TATGTGGAGG GAGGGCTGCG CCCCCTCGAA
    AGTGCCCTCT GCGCCCCCCT GGGTTTTGCC TCCTCGCTGC ACGGCCTGCA GATGTCCAAG
    AACATGAGTG ATGACATGAA GGAGGTGGCC TTCTCGGTCA GGAACGCCAT CTGCTCTGGC
    CCCCCCAAGC CTCCGCTCAA GGACATGGCC TGCCAGACCA ATGGGTCCCG GACGACGGGA
    ACACAGACCG TCCAGACCAT CACCATTGGC CTGCAGACCG AAGCCCTGCG TGGCAGCGCC
    GTCACCAGCA GCCCCCACAA GTGTCTCACA CCCAAGGCCG GGGGCGGGGC CACGCCCGCG
    TCATCTCCTT CCCGTGGCCT TCGGAGCCGG CAGATGGCCC CCGCCATCGA GAAGGTGCAG
    GCCAAGTTCG AACGCACGTG CTGCTCCCCC AAGTACGGGT CTCCCAAGCT GCAGAGGAAG
    CCCCTCCCCA AAGCTGAGCC GCCCAACAGC AGGACCTCCC TGGGGCTGGC CCCAAAGGGG
    TGCAGCGAGT CCGCCTGGGC CCGTTCCACC ACCACGAGGG AGAGCCCGGT GCACACCGCC
    ATCAACGATG GCCTCTCCAG CCTCTTCAAC ATCATCGACC ACAGCCCCGT GGTGCAGGAC
    CCCTTCCAGA AGGGGACTCG GGCCGGGAGC CGATCTCGCT CAGCAGAACC CCGGCCCGAG
    CTGGGCCCAG GCCAGGAAAC GGGCCCCAGT TCCCGGGGCC GGTCCCCCAG CCCCATCGGG
    GCTGGCTCAG AGACTTGGAG GGAGGAAGGG GGAGAGGGCA CGCCCTTGAG GCAGGACTTA
    TCCGCTCCCC CTGGTTACAC GTTCACTGAG AACGTAGCCC GGATCCTCAA CAAGAAGCTG
    TTGGAGCATG CCTTAAAGGA GGAGAGGAGG CAGGCCCCCC ACGGGCCCCC AAGTCTTAAC
    AGTGACAGCC ACATGGGAGA AACCATCAAA GCTGAACCAG GGTCCATCGA GAACCAAACT
    GTCTTACTAA CTGCCCCCTG GGAACTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005623137.1
    CDS365..5074
    Translation

    Target ORF information:

    RefSeq Version XM_005623080.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005623080.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG
    CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG
    TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG
    CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC
    ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG
    GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT
    CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG
    TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC
    CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG
    CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG
    TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC
    CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC
    CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG
    GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG
    GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA
    TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC
    GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG
    GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG
    GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG
    ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT
    GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC
    TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG
    CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG
    TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG
    CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG ATGGAGGAGG AGCACCTGTA CGCCTTGAGG
    TGGAAAGAAC TGGAAATGCA CAGCCTGGCT TTGCAGAACA CCCTCCATGA GCAAACCTGG
    AGTGATGAGA GGAACCTGAT GCAGCAGGAG CTCCGGTCTA TGAAGCAGAA CATTTTCCTT
    TTCTACGTCA AACTCAGATG GCTGCTGAGG CACTGGCGAC AAGGGAAGCA GATGGAGGAA
    GAAGGCGAGG ATTTCACTGA GAGCGAGCAT CCAGAGACCC TCCCCAGGCT TGGTGAGCTT
    GGAGTGCAGG GGGACCCGAA GGGGGACGGC CCAGACCAAG ATGACAACGG TCCGGGCTGC
    GGCTTTCCGC TGGGAGAGCA TCCTCCACAC TCCCCTGTGC AGATTGGTGA CCATGGATCG
    AGACTACAGC CTACACATGG GAGACAGCTC CACAGGCAGG TGGTGGAAAA CCAGCAGCTG
    TTCAGTGCAC TCAAGAGCCT GCTGGAGGAC CTGCGCGCCG AGCTGCGGGA GGACGAGCAT
    GCACGGCGGC GGCTGCAGCA GCAGTACGCC AGCGACAAAG CCGCCTGGGA CGTGGAGTGG
    GCTGCACTTA CCTGCCGCCT GGAACAGCTG GAAGAGAAGA CTGAGAAGAA TTTGGGAGAA
    GCATGCTCTT CCACCGACAG CAAAGGGGCA TTCAAGAAGG AAAGAGAGAT GCATCAGAAG
    CTCCTCACTG ACAGTCATGG CCTGGTCATG GACCTGCGCT GGCAGATCCA TCACAGTGAG
    AAGAACTGGA GCCGGGAGAA GGTGGAACTT CTTAGCCGTC TAGACAGAGA CCGGCAGGAA
    TGGGAGCAGC AGAAGAAGGA GCTCTTGTGG AGAATAGAAC AGTTGCAGAA AGAAAACAGT
    CCCCGGAGAA GTGGCAGTTT CCTCTGTGAT CAAAAAGAAG GCACCATCCG CCCCTTTGCC
    CATCCAGGAA GCCCCCGAAT GCCCCGTCCC TTGGGGATGT GGTCCTGTGC AGACACCGAC
    TCCACCCCAT TCGAAGACCG GCCGCTGTCC AAGCTGAAGG AATCAGACAG ATGTTCAGCA
    CGGGAAAACC TCTACTTGGA CGCCTTGTCT CTAGATGACG AGCCAGAGGA GCCTCCAGCC
    CGAGAGCCCC AGCGGGAGTT CAGGAACTGC CTCCCCCAGG AAGAAGAAAA TCACAAGGGA
    AATCTTCAAA GGGCCGTGTC TGTGTCCTCC ATGTCTGAGT TCCAGCGTCT GATGGACGTT
    TCTCCCTTCC TGCCGGAGAA GGGCGTCCCC GCCACCAGCA GCAAGGAGGA CATCACTCCG
    CCCCTGTCTC CTGATGATCT GAAGTACATC GAGGAGTTCA ACAGCAAGAA CTGGGACGAG
    AGGCCCCCGG AGCCCTGGGC AGACAGGACC GAGGGGGGGC GGGCAGGGAG CGAGGCTGGT
    GCAGAGCCTT TCCCTGACTC CTCCTGGTAC CTCACCACGA GTGTCACCAT GACTACGGAC
    ACCATGACCA GCCCAGAGCA CTGCCAGAAG CAGCCCCTTC GGAGCCCCAT GTGCGCGGAG
    CAGGCCGGGG TGCGGGTGCT GCACAGCCCG CCCGCGGTCC GTAGGATGGA TGGCATCGTG
    GTGGCGGGCA GTGAGGGCAA GGGCCGGGCC GAGCCCGAGT GCCCGTTTCC AACTAGCAGA
    GCCAGGGGGG GCACTGGAGA CACAAAAGGG GGTCCCTCAG AACACGTGCT CAGCAGGTGG
    CCTTGCGCCC CCTCCAGGCA CCCCCGAGAC TATGTGGAGG GAGGGCTGCG CCCCCTCGAA
    AGTGCCCTCT GCGCCCCCCT GGGTTTTGCC TCCTCGCTGC ACGGCCTGCA GATGTCCAAG
    AACATGAGTG ATGACATGAA GGAGGTGGCC TTCTCGGTCA GGAACGCCAT CTGCTCTGGC
    CCCCCCAAGC CTCCGCTCAA GGACATGGCC TGCCAGACCA ATGGGTCCCG GACGACGGGA
    ACACAGACCG TCCAGACCAT CACCATTGGC CTGCAGACCG AAGCCCTGCG TGGCAGCGCC
    GTCACCAGCA GCCCCCACAA GTGTCTCACA CCCAAGGCCG GGGGCGGGGC CACGCCCGCG
    TCATCTCCTT CCCGTGGCCT TCGGAGCCGG CAGATGGCCC CCGCCATCGA GAAGGTGCAG
    GCCAAGTTCG AACGCACGTG CTGCTCCCCC AAGTACGGGT CTCCCAAGCT GCAGAGGAAG
    CCCCTCCCCA AAGCTGAGCC GCCCAACAGC AGGACCTCCC TGGGGCTGGC CCCAAAGGGG
    TGCAGCGAGT CCGCCTGGGC CCGTTCCACC ACCACGAGGG AGAGCCCGGT GCACACCGCC
    ATCAACGATG GCCTCTCCAG CCTCTTCAAC ATCATCGACC ACAGCCCCGT GGTGCAGGAC
    CCCTTCCAGA AGGGGACTCG GGCCGGGAGC CGATCTCGCT CAGCAGAACC CCGGCCCGAG
    CTGGGCCCAG GCCAGGAAAC GGGCCCCAGT TCCCGGGGCC GGTCCCCCAG CCCCATCGGG
    GCTGGCTCAG AGACTTGGAG GGAGGAAGGG GGAGAGGGCA CGCCCTTGAG GCAGGACTTA
    TCCGCTCCCC CTGGTTACAC GTTCACTGAG AACGTAGCCC GGATCCTCAA CAAGAAGCTG
    TTGGAGCATG CCTTAAAGGA GGAGAGGAGG CAGGCCCCCC ACGGGCCCCC AAGTCTTAAC
    AGTGACAGCC ACATGGGAGA AACCATCAAA GCTGAACCAG GGTCCATCGA GAACCAAACT
    GTCTTACTAA CTGCCCCCTG GGAACTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe09965
    Clone ID Related Accession (Same CDS sequence) XM_005623080.2 , XM_005623080.3
    Accession Version XM_005623080.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product microtubule cross-linking factor 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005623080.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG
    CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG
    TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG
    CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC
    ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG
    GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT
    CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG
    TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC
    CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG
    CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG
    TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC
    CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC
    CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG
    GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG
    GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA
    TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC
    GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG
    GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG
    GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG
    ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT
    GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC
    TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG
    CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG
    TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG
    CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG ATGGAGGAGG AGCACCTGTA CGCCTTGAGG
    TGGAAAGAAC TGGAAATGCA CAGCCTGGCT TTGCAGAACA CCCTCCATGA GCAAACCTGG
    AGTGATGAGA GGAACCTGAT GCAGCAGGAG CTCCGGTCTA TGAAGCAGAA CATTTTCCTT
    TTCTACGTCA AACTCAGATG GCTGCTGAGG CACTGGCGAC AAGGGAAGCA GATGGAGGAA
    GAAGGCGAGG ATTTCACTGA GAGCGAGCAT CCAGAGACCC TCCCCAGGCT TGGTGAGCTT
    GGAGTGCAGG GGGACCCGAA GGGGGACGGC CCAGACCAAG ATGACAACGG TCCGGGCTGC
    GGCTTTCCGC TGGGAGAGCA TCCTCCACAC TCCCCTGTGC AGATTGGTGA CCATGGATCG
    AGACTACAGC CTACACATGG GAGACAGCTC CACAGGCAGG TGGTGGAAAA CCAGCAGCTG
    TTCAGTGCAC TCAAGAGCCT GCTGGAGGAC CTGCGCGCCG AGCTGCGGGA GGACGAGCAT
    GCACGGCGGC GGCTGCAGCA GCAGTACGCC AGCGACAAAG CCGCCTGGGA CGTGGAGTGG
    GCTGCACTTA CCTGCCGCCT GGAACAGCTG GAAGAGAAGA CTGAGAAGAA TTTGGGAGAA
    GCATGCTCTT CCACCGACAG CAAAGGGGCA TTCAAGAAGG AAAGAGAGAT GCATCAGAAG
    CTCCTCACTG ACAGTCATGG CCTGGTCATG GACCTGCGCT GGCAGATCCA TCACAGTGAG
    AAGAACTGGA GCCGGGAGAA GGTGGAACTT CTTAGCCGTC TAGACAGAGA CCGGCAGGAA
    TGGGAGCAGC AGAAGAAGGA GCTCTTGTGG AGAATAGAAC AGTTGCAGAA AGAAAACAGT
    CCCCGGAGAA GTGGCAGTTT CCTCTGTGAT CAAAAAGAAG GCACCATCCG CCCCTTTGCC
    CATCCAGGAA GCCCCCGAAT GCCCCGTCCC TTGGGGATGT GGTCCTGTGC AGACACCGAC
    TCCACCCCAT TCGAAGACCG GCCGCTGTCC AAGCTGAAGG AATCAGACAG ATGTTCAGCA
    CGGGAAAACC TCTACTTGGA CGCCTTGTCT CTAGATGACG AGCCAGAGGA GCCTCCAGCC
    CGAGAGCCCC AGCGGGAGTT CAGGAACTGC CTCCCCCAGG AAGAAGAAAA TCACAAGGGA
    AATCTTCAAA GGGCCGTGTC TGTGTCCTCC ATGTCTGAGT TCCAGCGTCT GATGGACGTT
    TCTCCCTTCC TGCCGGAGAA GGGCGTCCCC GCCACCAGCA GCAAGGAGGA CATCACTCCG
    CCCCTGTCTC CTGATGATCT GAAGTACATC GAGGAGTTCA ACAGCAAGAA CTGGGACGAG
    AGGCCCCCGG AGCCCTGGGC AGACAGGACC GAGGGGGGGC GGGCAGGGAG CGAGGCTGGT
    GCAGAGCCTT TCCCTGACTC CTCCTGGTAC CTCACCACGA GTGTCACCAT GACTACGGAC
    ACCATGACCA GCCCAGAGCA CTGCCAGAAG CAGCCCCTTC GGAGCCCCAT GTGCGCGGAG
    CAGGCCGGGG TGCGGGTGCT GCACAGCCCG CCCGCGGTCC GTAGGATGGA TGGCATCGTG
    GTGGCGGGCA GTGAGGGCAA GGGCCGGGCC GAGCCCGAGT GCCCGTTTCC AACTAGCAGA
    GCCAGGGGGG GCACTGGAGA CACAAAAGGG GGTCCCTCAG AACACGTGCT CAGCAGGTGG
    CCTTGCGCCC CCTCCAGGCA CCCCCGAGAC TATGTGGAGG GAGGGCTGCG CCCCCTCGAA
    AGTGCCCTCT GCGCCCCCCT GGGTTTTGCC TCCTCGCTGC ACGGCCTGCA GATGTCCAAG
    AACATGAGTG ATGACATGAA GGAGGTGGCC TTCTCGGTCA GGAACGCCAT CTGCTCTGGC
    CCCCCCAAGC CTCCGCTCAA GGACATGGCC TGCCAGACCA ATGGGTCCCG GACGACGGGA
    ACACAGACCG TCCAGACCAT CACCATTGGC CTGCAGACCG AAGCCCTGCG TGGCAGCGCC
    GTCACCAGCA GCCCCCACAA GTGTCTCACA CCCAAGGCCG GGGGCGGGGC CACGCCCGCG
    TCATCTCCTT CCCGTGGCCT TCGGAGCCGG CAGATGGCCC CCGCCATCGA GAAGGTGCAG
    GCCAAGTTCG AACGCACGTG CTGCTCCCCC AAGTACGGGT CTCCCAAGCT GCAGAGGAAG
    CCCCTCCCCA AAGCTGAGCC GCCCAACAGC AGGACCTCCC TGGGGCTGGC CCCAAAGGGG
    TGCAGCGAGT CCGCCTGGGC CCGTTCCACC ACCACGAGGG AGAGCCCGGT GCACACCGCC
    ATCAACGATG GCCTCTCCAG CCTCTTCAAC ATCATCGACC ACAGCCCCGT GGTGCAGGAC
    CCCTTCCAGA AGGGGACTCG GGCCGGGAGC CGATCTCGCT CAGCAGAACC CCGGCCCGAG
    CTGGGCCCAG GCCAGGAAAC GGGCCCCAGT TCCCGGGGCC GGTCCCCCAG CCCCATCGGG
    GCTGGCTCAG AGACTTGGAG GGAGGAAGGG GGAGAGGGCA CGCCCTTGAG GCAGGACTTA
    TCCGCTCCCC CTGGTTACAC GTTCACTGAG AACGTAGCCC GGATCCTCAA CAAGAAGCTG
    TTGGAGCATG CCTTAAAGGA GGAGAGGAGG CAGGCCCCCC ACGGGCCCCC AAGTCTTAAC
    AGTGACAGCC ACATGGGAGA AACCATCAAA GCTGAACCAG GGTCCATCGA GAACCAAACT
    GTCTTACTAA CTGCCCCCTG GGAACTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005623137.1
    CDS132..4841
    Translation

    Target ORF information:

    RefSeq Version XM_005623080.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005623080.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
    GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT
    CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA
    GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG
    AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT
    GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG
    ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG
    AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG
    GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG
    CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG
    TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG
    CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC
    ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG
    GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT
    CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG
    TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC
    CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG
    CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG
    TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC
    CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC
    CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG
    GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG
    GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA
    TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC
    GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG
    GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG
    GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG
    ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT
    GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC
    TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG
    CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG
    TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG
    CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG ATGGAGGAGG AGCACCTGTA CGCCTTGAGG
    TGGAAAGAAC TGGAAATGCA CAGCCTGGCT TTGCAGAACA CCCTCCATGA GCAAACCTGG
    AGTGATGAGA GGAACCTGAT GCAGCAGGAG CTCCGGTCTA TGAAGCAGAA CATTTTCCTT
    TTCTACGTCA AACTCAGATG GCTGCTGAGG CACTGGCGAC AAGGGAAGCA GATGGAGGAA
    GAAGGCGAGG ATTTCACTGA GAGCGAGCAT CCAGAGACCC TCCCCAGGCT TGGTGAGCTT
    GGAGTGCAGG GGGACCCGAA GGGGGACGGC CCAGACCAAG ATGACAACGG TCCGGGCTGC
    GGCTTTCCGC TGGGAGAGCA TCCTCCACAC TCCCCTGTGC AGATTGGTGA CCATGGATCG
    AGACTACAGC CTACACATGG GAGACAGCTC CACAGGCAGG TGGTGGAAAA CCAGCAGCTG
    TTCAGTGCAC TCAAGAGCCT GCTGGAGGAC CTGCGCGCCG AGCTGCGGGA GGACGAGCAT
    GCACGGCGGC GGCTGCAGCA GCAGTACGCC AGCGACAAAG CCGCCTGGGA CGTGGAGTGG
    GCTGCACTTA CCTGCCGCCT GGAACAGCTG GAAGAGAAGA CTGAGAAGAA TTTGGGAGAA
    GCATGCTCTT CCACCGACAG CAAAGGGGCA TTCAAGAAGG AAAGAGAGAT GCATCAGAAG
    CTCCTCACTG ACAGTCATGG CCTGGTCATG GACCTGCGCT GGCAGATCCA TCACAGTGAG
    AAGAACTGGA GCCGGGAGAA GGTGGAACTT CTTAGCCGTC TAGACAGAGA CCGGCAGGAA
    TGGGAGCAGC AGAAGAAGGA GCTCTTGTGG AGAATAGAAC AGTTGCAGAA AGAAAACAGT
    CCCCGGAGAA GTGGCAGTTT CCTCTGTGAT CAAAAAGAAG GCACCATCCG CCCCTTTGCC
    CATCCAGGAA GCCCCCGAAT GCCCCGTCCC TTGGGGATGT GGTCCTGTGC AGACACCGAC
    TCCACCCCAT TCGAAGACCG GCCGCTGTCC AAGCTGAAGG AATCAGACAG ATGTTCAGCA
    CGGGAAAACC TCTACTTGGA CGCCTTGTCT CTAGATGACG AGCCAGAGGA GCCTCCAGCC
    CGAGAGCCCC AGCGGGAGTT CAGGAACTGC CTCCCCCAGG AAGAAGAAAA TCACAAGGGA
    AATCTTCAAA GGGCCGTGTC TGTGTCCTCC ATGTCTGAGT TCCAGCGTCT GATGGACGTT
    TCTCCCTTCC TGCCGGAGAA GGGCGTCCCC GCCACCAGCA GCAAGGAGGA CATCACTCCG
    CCCCTGTCTC CTGATGATCT GAAGTACATC GAGGAGTTCA ACAGCAAGAA CTGGGACGAG
    AGGCCCCCGG AGCCCTGGGC AGACAGGACC GAGGGGGGGC GGGCAGGGAG CGAGGCTGGT
    GCAGAGCCTT TCCCTGACTC CTCCTGGTAC CTCACCACGA GTGTCACCAT GACTACGGAC
    ACCATGACCA GCCCAGAGCA CTGCCAGAAG CAGCCCCTTC GGAGCCCCAT GTGCGCGGAG
    CAGGCCGGGG TGCGGGTGCT GCACAGCCCG CCCGCGGTCC GTAGGATGGA TGGCATCGTG
    GTGGCGGGCA GTGAGGGCAA GGGCCGGGCC GAGCCCGAGT GCCCGTTTCC AACTAGCAGA
    GCCAGGGGGG GCACTGGAGA CACAAAAGGG GGTCCCTCAG AACACGTGCT CAGCAGGTGG
    CCTTGCGCCC CCTCCAGGCA CCCCCGAGAC TATGTGGAGG GAGGGCTGCG CCCCCTCGAA
    AGTGCCCTCT GCGCCCCCCT GGGTTTTGCC TCCTCGCTGC ACGGCCTGCA GATGTCCAAG
    AACATGAGTG ATGACATGAA GGAGGTGGCC TTCTCGGTCA GGAACGCCAT CTGCTCTGGC
    CCCCCCAAGC CTCCGCTCAA GGACATGGCC TGCCAGACCA ATGGGTCCCG GACGACGGGA
    ACACAGACCG TCCAGACCAT CACCATTGGC CTGCAGACCG AAGCCCTGCG TGGCAGCGCC
    GTCACCAGCA GCCCCCACAA GTGTCTCACA CCCAAGGCCG GGGGCGGGGC CACGCCCGCG
    TCATCTCCTT CCCGTGGCCT TCGGAGCCGG CAGATGGCCC CCGCCATCGA GAAGGTGCAG
    GCCAAGTTCG AACGCACGTG CTGCTCCCCC AAGTACGGGT CTCCCAAGCT GCAGAGGAAG
    CCCCTCCCCA AAGCTGAGCC GCCCAACAGC AGGACCTCCC TGGGGCTGGC CCCAAAGGGG
    TGCAGCGAGT CCGCCTGGGC CCGTTCCACC ACCACGAGGG AGAGCCCGGT GCACACCGCC
    ATCAACGATG GCCTCTCCAG CCTCTTCAAC ATCATCGACC ACAGCCCCGT GGTGCAGGAC
    CCCTTCCAGA AGGGGACTCG GGCCGGGAGC CGATCTCGCT CAGCAGAACC CCGGCCCGAG
    CTGGGCCCAG GCCAGGAAAC GGGCCCCAGT TCCCGGGGCC GGTCCCCCAG CCCCATCGGG
    GCTGGCTCAG AGACTTGGAG GGAGGAAGGG GGAGAGGGCA CGCCCTTGAG GCAGGACTTA
    TCCGCTCCCC CTGGTTACAC GTTCACTGAG AACGTAGCCC GGATCCTCAA CAAGAAGCTG
    TTGGAGCATG CCTTAAAGGA GGAGAGGAGG CAGGCCCCCC ACGGGCCCCC AAGTCTTAAC
    AGTGACAGCC ACATGGGAGA AACCATCAAA GCTGAACCAG GGTCCATCGA GAACCAAACT
    GTCTTACTAA CTGCCCCCTG GGAACTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.