CACNA1E gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_022420929.1	XP_022276637.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
XM_005622391.2	XP_005622448.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X3
XM_003434969.3	XP_003435017.2	voltage-dependent R-type calcium channel subunit alpha-1E isoform X10
XM_014115114.1	XP_013970589.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
XM_003434970.3	XP_003435018.2	voltage-dependent R-type calcium channel subunit alpha-1E isoform X9
XM_022420928.1	XP_022276636.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
XM_022420934.1	XP_022276642.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X7
XM_022420935.1	XP_022276643.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X8
XM_005622392.2	XP_005622449.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X4
XM_005622394.2	XP_005622451.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X8
XM_022420936.1	XP_022276644.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X9
XM_005622390.2	XP_005622447.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
XM_547425.5	XP_547425.4	voltage-dependent R-type calcium channel subunit alpha-1E isoform X5
XM_014115117.1	XP_013970592.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X13
XM_022420932.1	XP_022276640.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X5
XM_022420933.1	XP_022276641.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X6
XM_014115115.1	XP_013970590.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X6
XM_014115116.1	XP_013970591.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X11
XM_005622393.2	XP_005622450.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X7
XM_005622396.1	XP_005622453.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X12
XM_022420930.1	XP_022276638.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X3
XM_022420931.1	XP_022276639.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe09112	XM_005622390.2 Latest version!	Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe09113	XM_005622391.2 Latest version!	Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe09114	XM_005622392.2 Latest version!	Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe09115	XM_547425.5 Latest version!	Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe09116	XM_005622393.2 Latest version!	Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe09117	XM_005622394.2 Latest version!	Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe09118	XM_003434970.3 Latest version!	Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe09120	XM_003434969.3 Latest version!	Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe09121	XM_005622396.1 Latest version!	Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe289494	XM_022420928.1 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe289495	XM_022420929.1 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe289496	XM_022420930.1 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe289497	XM_022420931.1 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe289498	XM_022420932.1 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe289499	XM_022420933.1 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe289500	XM_022420934.1 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe289501	XM_022420935.1 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe289502	XM_022420936.1 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe37410	XM_014115114.1 Latest version!	Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe37411	XM_014115115.1 Latest version!	Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe37412	XM_014115116.1 Latest version!	Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe37413	XM_014115117.1 Latest version!	Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00

CACNA1E ( XM_005622390.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622447.1 Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe09112
Clone ID Related Accession (Same CDS sequence)	XM_005622390.2
Accession Version	XM_005622390.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005622390.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGC AATGTTCTGG GATGGTGCCC GAGATGCGTG GACCGAGCCC AGGCCGCAGG 
CTGCCCGAGC CCGCCCCCTG CCCCGCCGCG GCCGGGAGCC CTGAGCCCTG GGCCGCGGGC 
CGCCGCCGCC ACGTCCTGGC CCTGCCTGAG GAGGAGGCAT GGAGGCCCCA GCAGTGCTCC 
CTGCTCGAGG CCGACGCCTC GGACGAGGTG GGCATGCTGC CGGCCTCCCC GCGCGCCGCC 
GCCTCCGGCG TCGACAACTT CTTCCCGGTG CAGGAGGGCG AGGGCGCGGG CTGGGAGGGC 
GGTGCGGCCC TGGACGCCGG CGCCGGCCCC TTCCTCCCGG TGAGCCCCGA GGTGATGAAG 
CGGCGGCGCG GGGGCCTCAT CGAGCAGCGC GACATCATCA AGGCCCACGA GGCGCACAAG 
ATGCAGAGCA CCCCGCAGGC GCGGCGCAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG 
GTGGTCGGCA GGCCGGGGTC GGGCGATGGA GACTCGGACC AGAGCAGGAA CCGGCAAGGA 
ACCCCGGTGC CGGCCTCGGG GCCGGCGGCC GCCTACAAGC AGTCGAAAGC GCAGAGGGCG 
CGGACTATGG CTTTGTACAA CCCCATTCCC GTCCGGCAGA ACTGTTTCAC CGTCAACAGA 
TCCCTGTTCA TCTTCGGAGA AGATAACATT GTCAGGAAAT ACGCCAAGAA GCTCATCGAC 
TGGCCGCCGT TTGAGTACAT GATTTTGGCA ACCATCATTG CCAACTGCAT TGTCTTGGCC 
CTGGAACAGC ATCTTCCTGA GGATGACAAG ACCCCGATGT CCCGCAGACT GGAGAAGACA 
GAGCCGTATT TCATTGGGAT CTTTTGCTTT GAAGCCGGTA TCAAAATTGT GGCCCTGGGG 
TTCATCTTTC ATAAGGGCTC GTACCTCCGC AATGGCTGGA ATGTCATGGA CTTCATCGTG 
GTCCTCAGTG GCATCCTGGC CACTGCGGGG ACTCACTTCA ACACACACGT GGACCTGAGG 
ACCCTCCGGG CTGTGCGTGT CCTGAGACCC CTGAAGCTCG TGTCAGGGAT TCCTAGCCTG 
CAGATCGTGT TGAAGTCTAT CATGAAGGCC ATGGTGCCTC TTCTGCAAAT TGGTCTCCTG 
CTCTTCTTTG CCATCTTGAT GTTTGCTATC ATTGGCTTAG AGTTCTACAG TGGGAAGTTG 
CACCGAGCAT GTTTCACGAA CAATTCAGGT ATTCTGGAAG GATTTGACCC CCCTCACCCA 
TGTGGTGTGC AGGGCTGCCC AGCTGGCTAC GAATGCCGGG ACTGGATTGG CCCCAATGAT 
GGGATCACCC AGTTTGATAA CATCCTTTTT GCTGTGCTGA CTGTCTTCCA ATGCATCACC 
ATGGAAGGAT GGACCACCGT GCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC 
TGGCTGTACT TCATCCCCCT CATCATCATT GGATCCTTCT TTGTTCTCAA CCTAGTTCTG 
GGAGTGCTTT CCGGGGAGTT TGCCAAAGAG AGAGAGAGAG TGGAGAACCG GAGAGCTTTC 
ATGAAACTGC GGCGGCAGCA GCAGATTGAG CGGGAGCTGA ACGGGTACCG CGCCTGGATA 
GACAAAGCAG AGGAAGTCAT GCTTGCTGAA GAAAATAAAA ATGCTGGGAC GTCAGCCTTA 
GAAGTGCTTC GAAGGGCAAC CATCAAAAGG AGCCGGACAG AGGCCATGAC TCGAGACTCC 
AGTGATGAAC ACTGTGTCGA TATCTCCTCT GTGGGCACAC CTCTCGCCCG AGCCAGCATC 
AAAAGTGCAA AGGTGGATGG GGCCTCCTAT TTCCGGCACA AAGAAAGGCT TCTGCGCATC 
TCTATCCGCC ACATGGTGAA ATCCCAGGTG TTTTACTGGA TTGTGTTGAG CCTTGTGGCT 
CTCAACACTG CCTGTGTGGC CATCGTCCAT CATAACCAGC CCCAGTGGCT CACCCATCTC 
CTCTACTATG CAGAATTTTT GTTTCTGGGA CTGTTCCTCT TGGAGATGTC CCTGAAGATG 
TATGGCATGG GACCTCGACT TTATTTTCAC TCTTCATTCA ACTGTTTTGA TTTTGGGGTC 
ACAGTGGGCA GCATCTTTGA AGTGGTCTGG GCAATCTTCA GACCTGGCAC ATCTTTTGGA 
ATCAGTGTCT TACGGGCTCT CCGGCTTCTA AGAATATTTA AAATAACCAA GTATTGGGCA 
TCTCTGCGAA ATTTGGTGGT CTCCTTGATG AGCTCTATGA AGTCTATCAT CAGTTTGCTC 
TTCCTCCTCT TCCTCTTCAT CGTTGTCTTT GCTCTCCTAG GAATGCAGCT GTTTGGAGGC 
AGGTTTAACT TTAATGATGG GACTCCTTCA GCAAACTTTG ATACCTTCCC TGCAGCCATC 
ATGACTGTAT TCCAGATCCT AACAGGTGAG GACTGGAATG AGGTGATGTA CAATGGGATC 
CGTTCCCAGG GTGGAGTTAG CTCAGGCATG TGGTCAGCTA TCTATTTCAT TGTGCTCACC 
TTGTTTGGAA ACTACACACT GCTGAATGTG TTCTTGGCCA TTGCTGTGGA CAATCTGGCC 
AATGCCCAAG AGCTGACCAA GGATGAACAA GAAGAAGAGG AGGCTTTTAA CCAGAAACAT 
GCATTGCAGA AGGCCAAGGA GGTCAGCCCG ATGTCTGCAC CCAACATGCC TTCTATCGAA 
AGAGACAGAA GGAGAAGACA CCACATGTCG ATGTGGGAGC CACGCAGCAG CCACCTGAGG 
GAGCGGAGGC GCCGGCACCA CATGTCGGTG TGGGAGCAGC GCACCAGCCA GCTGAGAAGG 
CACATGCAGA TGTCCAGCCA GGAGGCCCTC AATAAAGAGG AGGCCCCACC GATGAACCCC 
CTCAACCCCC TCAACCCGCT GAGCCCCCTC AACCCGCTCA ACGCCCACCC CAGCCTCTAC 
CGGAGACCTA GGCCTATTGA GGGCCTGGGC CTGGGCCTGG AGAAGTGTGA GGAGGAGCGC 
ATCATCAGCC GCGGGGGGTC CCTCAAGGGG GACGGAGGGG ACCTGTCGAG TGCCCTGGAC 
AACCAGAGGA GCCCTTTGTC CCTGGGCAAA CGGGAGCCAC CATGGCTGGC CAGGCCCTGT 
CATGGGAACT GTGATCCGAC CCAGCAGGAG GCTGGGGGAG GGGAGACCGT GGTGACCTTC 
GAGGACCGGG CCAGGCACAG GCAGAGCCAG CGGCGCAGCC GGCACCGTCG TGTCAGGACG 
GAAGGCAAGG AGCTCTCCTC CACCTCCCGG AGCAGGTCTG TCAGCCAGGA GCGGAGTCTG 
GATGAGGGGG TGCTCACCGA CGGGGAGAAG GAGCGAGAGC CCAGGGGCGG CCATGCAGGC 
AAAGAACCCA CCATCCAAGA AGAAGACAGA GGCCAAGACT TAAGGAGGAC GAACAGTCTG 
ATGGTGCCCA GAGGCTCTGG GCTGGCAGGA GCCCTTGATG AGGCCAACAC ACCCCTAGTC 
CTGCCCCACC ACGATCTGGA AGTGGGGAAG GACACGGTGC TGACGGAGCA GGAGCCGGAG 
GGCAGCAGCG AGCAGGCCCT GCTGGGGGAT GTGCAGTTGG ACATTGGCAG GGTCATCAGC 
CAGAGTGAGC CTGACCTCTC CTGCGTCACA GCCAACACAG ACAAGGCCAC CACCGACAGC 
ACCAGCGTCA CCGTCGCCAT CCCTGATGTG GGCCCCTTGA TGGACTCAAC TGTGATTCAC 
ATTAGCAACA AAACTGATGG AGAAGCCAGT CCCTTGAAGG AGGCTGAGAT CAAAGAGGAG 
GAGGAGGAAA TGGAGAAGAA GCAGCAGAAG AAGGAGAAGC GTGAGACGGG CAAAGCCATG 
GTGCCCCACA GCTCTATGTT CATCTTCAGC ACCACCAACC CGATTCGCAG GGCCTGCCAC 
TACATCGTGA ACCTGCGCTA CTTTGAGATG TGCATTCTCC TGGTGATCGC CGCCAGCAGC 
ATTGCCCTGG CAGCAGAGGA CCCCGTCCTG ACCAACTCGG AGCGCAACAA AGTGGGTTCA 
CGGGAGCCGT TTGCCAACCT CCGTAATGCC CTGGTCCTGA GGTATTTTGA CTATGTTTTC 
ACTGGCGTGT TCACCTTTGA GATGGTTATA AAGATGATAG ACCAGGGCTT GATCCTACAG 
GATGGGTCCT ACTTCCGAGA CCTGTGGAAC ATCCTGGACT TTGTGGTGGT GGTTGGTGCA 
TTGGTGGCCT TTGCTTTGGC GAACGCTTTG GGAACAAACA AGGGGCGAGA CATCAAGACC 
ATCAAATCCC TGAGGGTGCT CCGAGTTCTG AGGCCACTGA AGACCATCAA GCGCTTGCCC 
AAACTCAAGG CTGTCTTTGA CTGTGTGGTG ACCTCCTTGA AGAATGTCTT CAACATACTC 
ATCGTCTATA AGCTCTTCAT GTTCATCTTT GCTGTCATTG CGGTTCAGCT CTTCAAGGGA 
AAATTCTTTT ATTGCACAGA CAGTTCCAAG GACACAGAGA AGGAGTGCAT AGGCAACTAC 
GTAGATCATG AAAAAAACAA GATGGAGGTG AAAGGCCGGG AATGGAAGCG CCATGAATTC 
CACTACGACA ACATTATCTG GGCCCTGCTG ACCCTCTTCA CCGTCTCCAC GGGGGAAGGA 
TGGCCTCAAG TCTTACAGCA TTCTGTCGAT GTGACAGAGG AAGACCGAGG ACCAAGCCGC 
AGCAACCGCA TGGAGATGTC CATCTTCTAT GTGGTCTACT TTGTGGTCTT CCCTTTCTTC 
TTTGTCAATA TCTTTGTGGC TCTGATCATC ATCACCTTCC AGGAACAAGG GGACAAGATG 
ATGGAGGAAT GCAGCCTGGA GAAGAACGAG AGGGCGTGCA TTGACTTCGC CATCAGTGCC 
AAACCTCTCA CCCGCTACAT GCCACAGAAC AGGCACACCT TCCAGTACCG CGTCTGGCAC 
TTCGTGGTGT CTCCGTCCTT TGAGTACACC ATCATGGCCA TGATCGCCCT GAACACCGTC 
GTGCTGATGA TGAAGTACTA CTCTGCTCCC TGTACCTATG AACTGGCCCT GAAGTACCTG 
AATATTGCCT TCACCATGGT GTTTTCCCTG GAATGTGTCC TAAAGATCAT CGCTTTTGGC 
TTCTTGAACT ACTTTCGAGA CACCTGGAAC ATCTTTGACT TCATCACTGT GATTGGCAGT 
ATCACAGAAA TCATACTGAC AGACAGCAAG CTGGTAAACA CCAGTGGTTT CAACATGAGC 
TTTCTGAAGC TCTTCCGTGC CGCCCGCCTC ATCAAGCTTC TGCGGCAGGG CTACACCATC 
CGTATTTTAC TCTGGACCTT CGTGCAGTCC TTTAAGGCCC TCCCCTACGT CTGCCTTTTG 
ATTGCCATGC TTTTCTTCAT CTATGCCATC ATTGGGATGC AGGTATTTGG AAACATAAAA 
TTAGATGAGG AGAGTCACAT CAACCGGCAC AACAACTTCC GGAGTTTCTT TGGGTCCCTC 
ATGTTACTCT TCAGGAGTGC CACGGGAGAG GCCTGGCAGG AGATAATGCT GTCGTGCCTC 
GGGGAGAAAG GCTGCGAGCC CGACACCACT GCGCCATCGG GGCAGAACGA GAATGAGCGC 
TGTGGCACCG ATCTGGCCTA TGTTTACTTT GTCTCCTTCA TCTTCTTCTG CTCTTTCTTG 
ATGCTCAACT TGTTTGTGGC CGTCATCATG GACAACTTCG AGTACCTGAC TCGGGACTCC 
TCCATCTTAG GGCCCCACCA CTTGGATGAG TTTGTCCGCG TCTGGGCAGA GTACGACCGA 
GCTGCATGTG GCCGCATCCA TTATACTGAG ATGTATGAAA TGCTGACTCT CATGTCACCA 
CCGCTAGGCC TCGGCAAGAG ATGTCCCTCC AAAGTGGCAT ATAAGAGGTT GGTCCTAATG 
AATATGCCAG TAGCTGAGGA CATGACAGTC CACTTCACCT CCACACTGAT GGCTCTGATC 
CGGACAGCCC TGGACATCAA AATCGCCAAA GGTGGTGCAG ACAGGCAGCA GCTAGACTCG 
GAGCTGCAAA AGGAGACCCT GGCCATCTGG CCTCACCTGT CCCAGAAGAT GCTAGACCTG 
CTTGTGCCCA TGCCCAAAGC CTCTGACCTG ACTGTGGGCA AAATCTATGC AGCAATGATG 
ATCATGGACT ACTATAAACA GAGTAAGGTG AAGAAACAGA GGCAGCAGCT GGAGGAACAG 
AAAAATGCAC CCATGTTCCA GCGAATGGAG CCCTCATCTC TGCCTCAGGA GATCATTGCT 
AATGCCAAAG CCCTGCCCTA TCTCCAGCAG GACCCCGTTT CAGGCCTGAG TGGCCGTAGT 
GGATACCCTT CGATGAGTCC ACTCTCCCCC CAGGAAATAT TCCAATTGGC TTGTATGGAT 
CCAGCCGATG ATGGACAGTT CCAGGAACAT CAGTCTCTGG AGCCAGAGGT TAGTGAATTA 
AAAAGCGTGC AGTCCTCTAA CCATGGCATC TACCTTCCTT CGGACACCCA GGAGCATACG 
GGATCTGGGA GGGCATCTTC TATGCCACGC CTGACTGTGG ATCCCCAGGT GGTGACAGAT 
ACTAGCTCCA TGAGACGTTC ATTTTCCACT ATTCGGGATA AGCGTTCAAA TTCCTCCTGG 
TTGGAGGAAT TCTCCATGGA GAGAAGCAGT GAAAACACCT ACAAGTCACG TCGTCGGAGT 
TACCACTCCT CCCTGAGACT GTCAGCTCAT CGCCTGAATT CTGACTCAGG CCACAAGTCT 
GACACCCACC GCTCAGGGGG TAGAGAGCGG GGACGATCGA AAGAGCGAAA GCATCTTCTC 
TCTCCTGATG TCTCCCGCTG CAACTCAGAG GAGCGAGGGA CCCAGGCCGA CTGGGAGTCC 
CCAGAGCGCC AACAGTCCAG GTCTCCCAGT GAGGCCAGGT CACAGACCCC CAACAGACAG 
GGCACGGGCT CCCTGAGCGA GAGCTCCATC CCCTCCATCT CTGACACCAG CACCCCAAGA 
CGAAGTCGCC GGCAGCTCCC ACCCGTCCCA CCAAAGCCTC GGCCCCTCCT CTCCTACAGC 
TCCCTGATTC GACACTCCGG GAGCATCTCC CCGCCGGCCG ACGGAAGCGA GAGGGGCTCC 
CCGCTGACCC CCCAGGCGCT GGAGAGCACG GAGGCCGGCC TGGCGGAGCC CTCGGGCCCC 
CGGCACGGGC ACGGGCAGGG GCACGGGCAG GGGCACGGGC AGCAGAGCCA GCACTGCTCC 
CCGCAGCGCT ACATCTCCGA GCCCTACCTG GCCCTGCACG AAGACTCCCA CGCGTCGGAC 
TGTGGCGAGG AGGAGACGCT CACCTTCGAG GCCGCCGTGG CCACCAGCCT GGGCCGCTCC 
AACACCATCG GCTCGGCCCC GCCGCTGCGG CACAGCTGGC AGATGCCCAA CGGGCACTAT 
CGGCGGCGGC GGCGCGGGGG GCCGGGGCCG GGCGTGATGT GCGGGGCGGT CAGCGACCTC 
CTGAGTGACA CGGAAGAAGA CGACAAGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622447.1
CDS	86..7558
Translation	MEEQCSGMVPEMRGPSPGRRLPEPAPCPAAAGSPEPWAAGRRRHVLALPEEEAWRPQQCSLLEADASDEVGMLPASPRAAASGVDNFFPVQEGEGAGWEGGAALDAGAGPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFTNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLGLGLEKCEEERIISRGGSLKGDGGDLSSALDNQRSPLSLGKREPPWLARPCHGNCDPTQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKELSSTSRSRSVSQERSLDEGVLTDGEKEREPRGGHAGKEPTIQEEDRGQDLRRTNSLMVPRGSGLAGALDEANTPLVLPHHDLEVGKDTVLTEQEPEGSSEQALLGDVQLDIGRVISQSEPDLSCVTANTDKATTDSTSVTVAIPDVGPLMDSTVIHISNKTDGEASPLKEAEIKEEEEEMEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVGSREPFANLRNALVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEHQSLEPEVSELKSVQSSNHGIYLPSDTQEHTGSGRASSMPRLTVDPQVVTDTSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERQQSRSPSEARSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHSGSISPPADGSERGSPLTPQALESTEAGLAEPSGPRHGHGQGHGQGHGQQSQHCSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGVMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_005622390.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622390.2

ORF Insert Sequence:

ATGGAAGAGC AATGTTCTGG GATGGTGCCC GAGATGCGTG GACCGAGCCC AGGCCGCAGG 
CTGCCCGAGC CCGCCCCCTG CCCCGCCGCG GCCGGGAGCC CTGAGCCCTG GGCCGCGGGC 
CGCCGCCGCC ACGTCCTGGC CCTGCCTGAG GAGGAGGCAT GGAGGCCCCA GCAGTGCTCC 
CTGCTCGAGG CCGACGCCTC GGACGAGGTG GGCATGCTGC CGGCCTCCCC GCGCGCCGCC 
GCCTCCGGCG TCGACAACTT CTTCCCGGTG CAGGAGGGCG AGGGCGCGGG CTGGGAGGGC 
GGTGCGGCCC TGGACGCCGG CGCCGGCCCC TTCCTCCCGG TGAGCCCCGA GGTGATGAAG 
CGGCGGCGCG GGGGCCTCAT CGAGCAGCGC GACATCATCA AGGCCCACGA GGCGCACAAG 
ATGCAGAGCA CCCCGCAGGC GCGGCGCAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG 
GTGGTCGGCA GGCCGGGGTC GGGCGATGGA GACTCGGACC AGAGCAGGAA CCGGCAAGGA 
ACCCCGGTGC CGGCCTCGGG GCCGGCGGCC GCCTACAAGC AGTCGAAAGC GCAGAGGGCG 
CGGACTATGG CTTTGTACAA CCCCATTCCC GTCCGGCAGA ACTGTTTCAC CGTCAACAGA 
TCCCTGTTCA TCTTCGGAGA AGATAACATT GTCAGGAAAT ACGCCAAGAA GCTCATCGAC 
TGGCCGCCGT TTGAGTACAT GATTTTGGCA ACCATCATTG CCAACTGCAT TGTCTTGGCC 
CTGGAACAGC ATCTTCCTGA GGATGACAAG ACCCCGATGT CCCGCAGACT GGAGAAGACA 
GAGCCGTATT TCATTGGGAT CTTTTGCTTT GAAGCCGGTA TCAAAATTGT GGCCCTGGGG 
TTCATCTTTC ATAAGGGCTC GTACCTCCGC AATGGCTGGA ATGTCATGGA CTTCATCGTG 
GTCCTCAGTG GCATCCTGGC CACTGCGGGG ACTCACTTCA ACACACACGT GGACCTGAGG 
ACCCTCCGGG CTGTGCGTGT CCTGAGACCC CTGAAGCTCG TGTCAGGGAT TCCTAGCCTG 
CAGATCGTGT TGAAGTCTAT CATGAAGGCC ATGGTGCCTC TTCTGCAAAT TGGTCTCCTG 
CTCTTCTTTG CCATCTTGAT GTTTGCTATC ATTGGCTTAG AGTTCTACAG TGGGAAGTTG 
CACCGAGCAT GTTTCACGAA CAATTCAGGT ATTCTGGAAG GATTTGACCC CCCTCACCCA 
TGTGGTGTGC AGGGCTGCCC AGCTGGCTAC GAATGCCGGG ACTGGATTGG CCCCAATGAT 
GGGATCACCC AGTTTGATAA CATCCTTTTT GCTGTGCTGA CTGTCTTCCA ATGCATCACC 
ATGGAAGGAT GGACCACCGT GCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC 
TGGCTGTACT TCATCCCCCT CATCATCATT GGATCCTTCT TTGTTCTCAA CCTAGTTCTG 
GGAGTGCTTT CCGGGGAGTT TGCCAAAGAG AGAGAGAGAG TGGAGAACCG GAGAGCTTTC 
ATGAAACTGC GGCGGCAGCA GCAGATTGAG CGGGAGCTGA ACGGGTACCG CGCCTGGATA 
GACAAAGCAG AGGAAGTCAT GCTTGCTGAA GAAAATAAAA ATGCTGGGAC GTCAGCCTTA 
GAAGTGCTTC GAAGGGCAAC CATCAAAAGG AGCCGGACAG AGGCCATGAC TCGAGACTCC 
AGTGATGAAC ACTGTGTCGA TATCTCCTCT GTGGGCACAC CTCTCGCCCG AGCCAGCATC 
AAAAGTGCAA AGGTGGATGG GGCCTCCTAT TTCCGGCACA AAGAAAGGCT TCTGCGCATC 
TCTATCCGCC ACATGGTGAA ATCCCAGGTG TTTTACTGGA TTGTGTTGAG CCTTGTGGCT 
CTCAACACTG CCTGTGTGGC CATCGTCCAT CATAACCAGC CCCAGTGGCT CACCCATCTC 
CTCTACTATG CAGAATTTTT GTTTCTGGGA CTGTTCCTCT TGGAGATGTC CCTGAAGATG 
TATGGCATGG GACCTCGACT TTATTTTCAC TCTTCATTCA ACTGTTTTGA TTTTGGGGTC 
ACAGTGGGCA GCATCTTTGA AGTGGTCTGG GCAATCTTCA GACCTGGCAC ATCTTTTGGA 
ATCAGTGTCT TACGGGCTCT CCGGCTTCTA AGAATATTTA AAATAACCAA GTATTGGGCA 
TCTCTGCGAA ATTTGGTGGT CTCCTTGATG AGCTCTATGA AGTCTATCAT CAGTTTGCTC 
TTCCTCCTCT TCCTCTTCAT CGTTGTCTTT GCTCTCCTAG GAATGCAGCT GTTTGGAGGC 
AGGTTTAACT TTAATGATGG GACTCCTTCA GCAAACTTTG ATACCTTCCC TGCAGCCATC 
ATGACTGTAT TCCAGATCCT AACAGGTGAG GACTGGAATG AGGTGATGTA CAATGGGATC 
CGTTCCCAGG GTGGAGTTAG CTCAGGCATG TGGTCAGCTA TCTATTTCAT TGTGCTCACC 
TTGTTTGGAA ACTACACACT GCTGAATGTG TTCTTGGCCA TTGCTGTGGA CAATCTGGCC 
AATGCCCAAG AGCTGACCAA GGATGAACAA GAAGAAGAGG AGGCTTTTAA CCAGAAACAT 
GCATTGCAGA AGGCCAAGGA GGTCAGCCCG ATGTCTGCAC CCAACATGCC TTCTATCGAA 
AGAGACAGAA GGAGAAGACA CCACATGTCG ATGTGGGAGC CACGCAGCAG CCACCTGAGG 
GAGCGGAGGC GCCGGCACCA CATGTCGGTG TGGGAGCAGC GCACCAGCCA GCTGAGAAGG 
CACATGCAGA TGTCCAGCCA GGAGGCCCTC AATAAAGAGG AGGCCCCACC GATGAACCCC 
CTCAACCCCC TCAACCCGCT GAGCCCCCTC AACCCGCTCA ACGCCCACCC CAGCCTCTAC 
CGGAGACCTA GGCCTATTGA GGGCCTGGGC CTGGGCCTGG AGAAGTGTGA GGAGGAGCGC 
ATCATCAGCC GCGGGGGGTC CCTCAAGGGG GACGGAGGGG ACCTGTCGAG TGCCCTGGAC 
AACCAGAGGA GCCCTTTGTC CCTGGGCAAA CGGGAGCCAC CATGGCTGGC CAGGCCCTGT 
CATGGGAACT GTGATCCGAC CCAGCAGGAG GCTGGGGGAG GGGAGACCGT GGTGACCTTC 
GAGGACCGGG CCAGGCACAG GCAGAGCCAG CGGCGCAGCC GGCACCGTCG TGTCAGGACG 
GAAGGCAAGG AGCTCTCCTC CACCTCCCGG AGCAGGTCTG TCAGCCAGGA GCGGAGTCTG 
GATGAGGGGG TGCTCACCGA CGGGGAGAAG GAGCGAGAGC CCAGGGGCGG CCATGCAGGC 
AAAGAACCCA CCATCCAAGA AGAAGACAGA GGCCAAGACT TAAGGAGGAC GAACAGTCTG 
ATGGTGCCCA GAGGCTCTGG GCTGGCAGGA GCCCTTGATG AGGCCAACAC ACCCCTAGTC 
CTGCCCCACC ACGATCTGGA AGTGGGGAAG GACACGGTGC TGACGGAGCA GGAGCCGGAG 
GGCAGCAGCG AGCAGGCCCT GCTGGGGGAT GTGCAGTTGG ACATTGGCAG GGTCATCAGC 
CAGAGTGAGC CTGACCTCTC CTGCGTCACA GCCAACACAG ACAAGGCCAC CACCGACAGC 
ACCAGCGTCA CCGTCGCCAT CCCTGATGTG GGCCCCTTGA TGGACTCAAC TGTGATTCAC 
ATTAGCAACA AAACTGATGG AGAAGCCAGT CCCTTGAAGG AGGCTGAGAT CAAAGAGGAG 
GAGGAGGAAA TGGAGAAGAA GCAGCAGAAG AAGGAGAAGC GTGAGACGGG CAAAGCCATG 
GTGCCCCACA GCTCTATGTT CATCTTCAGC ACCACCAACC CGATTCGCAG GGCCTGCCAC 
TACATCGTGA ACCTGCGCTA CTTTGAGATG TGCATTCTCC TGGTGATCGC CGCCAGCAGC 
ATTGCCCTGG CAGCAGAGGA CCCCGTCCTG ACCAACTCGG AGCGCAACAA AGTGGGTTCA 
CGGGAGCCGT TTGCCAACCT CCGTAATGCC CTGGTCCTGA GGTATTTTGA CTATGTTTTC 
ACTGGCGTGT TCACCTTTGA GATGGTTATA AAGATGATAG ACCAGGGCTT GATCCTACAG 
GATGGGTCCT ACTTCCGAGA CCTGTGGAAC ATCCTGGACT TTGTGGTGGT GGTTGGTGCA 
TTGGTGGCCT TTGCTTTGGC GAACGCTTTG GGAACAAACA AGGGGCGAGA CATCAAGACC 
ATCAAATCCC TGAGGGTGCT CCGAGTTCTG AGGCCACTGA AGACCATCAA GCGCTTGCCC 
AAACTCAAGG CTGTCTTTGA CTGTGTGGTG ACCTCCTTGA AGAATGTCTT CAACATACTC 
ATCGTCTATA AGCTCTTCAT GTTCATCTTT GCTGTCATTG CGGTTCAGCT CTTCAAGGGA 
AAATTCTTTT ATTGCACAGA CAGTTCCAAG GACACAGAGA AGGAGTGCAT AGGCAACTAC 
GTAGATCATG AAAAAAACAA GATGGAGGTG AAAGGCCGGG AATGGAAGCG CCATGAATTC 
CACTACGACA ACATTATCTG GGCCCTGCTG ACCCTCTTCA CCGTCTCCAC GGGGGAAGGA 
TGGCCTCAAG TCTTACAGCA TTCTGTCGAT GTGACAGAGG AAGACCGAGG ACCAAGCCGC 
AGCAACCGCA TGGAGATGTC CATCTTCTAT GTGGTCTACT TTGTGGTCTT CCCTTTCTTC 
TTTGTCAATA TCTTTGTGGC TCTGATCATC ATCACCTTCC AGGAACAAGG GGACAAGATG 
ATGGAGGAAT GCAGCCTGGA GAAGAACGAG AGGGCGTGCA TTGACTTCGC CATCAGTGCC 
AAACCTCTCA CCCGCTACAT GCCACAGAAC AGGCACACCT TCCAGTACCG CGTCTGGCAC 
TTCGTGGTGT CTCCGTCCTT TGAGTACACC ATCATGGCCA TGATCGCCCT GAACACCGTC 
GTGCTGATGA TGAAGTACTA CTCTGCTCCC TGTACCTATG AACTGGCCCT GAAGTACCTG 
AATATTGCCT TCACCATGGT GTTTTCCCTG GAATGTGTCC TAAAGATCAT CGCTTTTGGC 
TTCTTGAACT ACTTTCGAGA CACCTGGAAC ATCTTTGACT TCATCACTGT GATTGGCAGT 
ATCACAGAAA TCATACTGAC AGACAGCAAG CTGGTAAACA CCAGTGGTTT CAACATGAGC 
TTTCTGAAGC TCTTCCGTGC CGCCCGCCTC ATCAAGCTTC TGCGGCAGGG CTACACCATC 
CGTATTTTAC TCTGGACCTT CGTGCAGTCC TTTAAGGCCC TCCCCTACGT CTGCCTTTTG 
ATTGCCATGC TTTTCTTCAT CTATGCCATC ATTGGGATGC AGGTATTTGG AAACATAAAA 
TTAGATGAGG AGAGTCACAT CAACCGGCAC AACAACTTCC GGAGTTTCTT TGGGTCCCTC 
ATGTTACTCT TCAGGAGTGC CACGGGAGAG GCCTGGCAGG AGATAATGCT GTCGTGCCTC 
GGGGAGAAAG GCTGCGAGCC CGACACCACT GCGCCATCGG GGCAGAACGA GAATGAGCGC 
TGTGGCACCG ATCTGGCCTA TGTTTACTTT GTCTCCTTCA TCTTCTTCTG CTCTTTCTTG 
ATGCTCAACT TGTTTGTGGC CGTCATCATG GACAACTTCG AGTACCTGAC TCGGGACTCC 
TCCATCTTAG GGCCCCACCA CTTGGATGAG TTTGTCCGCG TCTGGGCAGA GTACGACCGA 
GCTGCATGTG GCCGCATCCA TTATACTGAG ATGTATGAAA TGCTGACTCT CATGTCACCA 
CCGCTAGGCC TCGGCAAGAG ATGTCCCTCC AAAGTGGCAT ATAAGAGGTT GGTCCTAATG 
AATATGCCAG TAGCTGAGGA CATGACAGTC CACTTCACCT CCACACTGAT GGCTCTGATC 
CGGACAGCCC TGGACATCAA AATCGCCAAA GGTGGTGCAG ACAGGCAGCA GCTAGACTCG 
GAGCTGCAAA AGGAGACCCT GGCCATCTGG CCTCACCTGT CCCAGAAGAT GCTAGACCTG 
CTTGTGCCCA TGCCCAAAGC CTCTGACCTG ACTGTGGGCA AAATCTATGC AGCAATGATG 
ATCATGGACT ACTATAAACA GAGTAAGGTG AAGAAACAGA GGCAGCAGCT GGAGGAACAG 
AAAAATGCAC CCATGTTCCA GCGAATGGAG CCCTCATCTC TGCCTCAGGA GATCATTGCT 
AATGCCAAAG CCCTGCCCTA TCTCCAGCAG GACCCCGTTT CAGGCCTGAG TGGCCGTAGT 
GGATACCCTT CGATGAGTCC ACTCTCCCCC CAGGAAATAT TCCAATTGGC TTGTATGGAT 
CCAGCCGATG ATGGACAGTT CCAGGAACAT CAGTCTCTGG AGCCAGAGGT TAGTGAATTA 
AAAAGCGTGC AGTCCTCTAA CCATGGCATC TACCTTCCTT CGGACACCCA GGAGCATACG 
GGATCTGGGA GGGCATCTTC TATGCCACGC CTGACTGTGG ATCCCCAGGT GGTGACAGAT 
ACTAGCTCCA TGAGACGTTC ATTTTCCACT ATTCGGGATA AGCGTTCAAA TTCCTCCTGG 
TTGGAGGAAT TCTCCATGGA GAGAAGCAGT GAAAACACCT ACAAGTCACG TCGTCGGAGT 
TACCACTCCT CCCTGAGACT GTCAGCTCAT CGCCTGAATT CTGACTCAGG CCACAAGTCT 
GACACCCACC GCTCAGGGGG TAGAGAGCGG GGACGATCGA AAGAGCGAAA GCATCTTCTC 
TCTCCTGATG TCTCCCGCTG CAACTCAGAG GAGCGAGGGA CCCAGGCCGA CTGGGAGTCC 
CCAGAGCGCC AACAGTCCAG GTCTCCCAGT GAGGCCAGGT CACAGACCCC CAACAGACAG 
GGCACGGGCT CCCTGAGCGA GAGCTCCATC CCCTCCATCT CTGACACCAG CACCCCAAGA 
CGAAGTCGCC GGCAGCTCCC ACCCGTCCCA CCAAAGCCTC GGCCCCTCCT CTCCTACAGC 
TCCCTGATTC GACACTCCGG GAGCATCTCC CCGCCGGCCG ACGGAAGCGA GAGGGGCTCC 
CCGCTGACCC CCCAGGCGCT GGAGAGCACG GAGGCCGGCC TGGCGGAGCC CTCGGGCCCC 
CGGCACGGGC ACGGGCAGGG GCACGGGCAG GGGCACGGGC AGCAGAGCCA GCACTGCTCC 
CCGCAGCGCT ACATCTCCGA GCCCTACCTG GCCCTGCACG AAGACTCCCA CGCGTCGGAC 
TGTGGCGAGG AGGAGACGCT CACCTTCGAG GCCGCCGTGG CCACCAGCCT GGGCCGCTCC 
AACACCATCG GCTCGGCCCC GCCGCTGCGG CACAGCTGGC AGATGCCCAA CGGGCACTAT 
CGGCGGCGGC GGCGCGGGGG GCCGGGGCCG GGCGTGATGT GCGGGGCGGT CAGCGACCTC 
CTGAGTGACA CGGAAGAAGA CGACAAGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_005622391.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622448.1 Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe09113
Clone ID Related Accession (Same CDS sequence)	XM_005622391.2
Accession Version	XM_005622391.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005622391.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGC AATGTTCTGG GATGGTGCCC GAGATGCGTG GACCGAGCCC AGGCCGCAGG 
CTGCCCGAGC CCGCCCCCTG CCCCGCCGCG GCCGGGAGCC CTGAGCCCTG GGCCGCGGGC 
CGCCGCCGCC ACGTCCTGGC CCTGCCTGAG GAGGAGGCAT GGAGGCCCCA GCAGTGCTCC 
CTGCTCGAGG CCGACGCCTC GGACGAGGTG GGCATGCTGC CGGCCTCCCC GCGCGCCGCC 
GCCTCCGGCG TCGACAACTT CTTCCCGGTG CAGGAGGGCG AGGGCGCGGG CTGGGAGGGC 
GGTGCGGCCC TGGACGCCGG CGCCGGCCCC TTCCTCCCGG TGAGCCCCGA GGTGATGAAG 
CGGCGGCGCG GGGGCCTCAT CGAGCAGCGC GACATCATCA AGGCCCACGA GGCGCACAAG 
ATGCAGAGCA CCCCGCAGGC GCGGCGCAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG 
GTGGTCGGCA GGCCGGGGTC GGGCGATGGA GACTCGGACC AGAGCAGGAA CCGGCAAGGA 
ACCCCGGTGC CGGCCTCGGG GCCGGCGGCC GCCTACAAGC AGTCGAAAGC GCAGAGGGCG 
CGGACTATGG CTTTGTACAA CCCCATTCCC GTCCGGCAGA ACTGTTTCAC CGTCAACAGA 
TCCCTGTTCA TCTTCGGAGA AGATAACATT GTCAGGAAAT ACGCCAAGAA GCTCATCGAC 
TGGCCGCCGT TTGAGTACAT GATTTTGGCA ACCATCATTG CCAACTGCAT TGTCTTGGCC 
CTGGAACAGC ATCTTCCTGA GGATGACAAG ACCCCGATGT CCCGCAGACT GGAGAAGACA 
GAGCCGTATT TCATTGGGAT CTTTTGCTTT GAAGCCGGTA TCAAAATTGT GGCCCTGGGG 
TTCATCTTTC ATAAGGGCTC GTACCTCCGC AATGGCTGGA ATGTCATGGA CTTCATCGTG 
GTCCTCAGTG GCATCCTGGC CACTGCGGGG ACTCACTTCA ACACACACGT GGACCTGAGG 
ACCCTCCGGG CTGTGCGTGT CCTGAGACCC CTGAAGCTCG TGTCAGGGAT TCCTAGCCTG 
CAGATCGTGT TGAAGTCTAT CATGAAGGCC ATGGTGCCTC TTCTGCAAAT TGGTCTCCTG 
CTCTTCTTTG CCATCTTGAT GTTTGCTATC ATTGGCTTAG AGTTCTACAG TGGGAAGTTG 
CACCGAGCAT GTTTCACGAA CAATTCAGGT ATTCTGGAAG GATTTGACCC CCCTCACCCA 
TGTGGTGTGC AGGGCTGCCC AGCTGGCTAC GAATGCCGGG ACTGGATTGG CCCCAATGAT 
GGGATCACCC AGTTTGATAA CATCCTTTTT GCTGTGCTGA CTGTCTTCCA ATGCATCACC 
ATGGAAGGAT GGACCACCGT GCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC 
TGGCTGTACT TCATCCCCCT CATCATCATT GGATCCTTCT TTGTTCTCAA CCTAGTTCTG 
GGAGTGCTTT CCGGGGAGTT TGCCAAAGAG AGAGAGAGAG TGGAGAACCG GAGAGCTTTC 
ATGAAACTGC GGCGGCAGCA GCAGATTGAG CGGGAGCTGA ACGGGTACCG CGCCTGGATA 
GACAAAGCAG AGGAAGTCAT GCTTGCTGAA GAAAATAAAA ATGCTGGGAC GTCAGCCTTA 
GAAGTGCTTC GAAGGGCAAC CATCAAAAGG AGCCGGACAG AGGCCATGAC TCGAGACTCC 
AGTGATGAAC ACTGTGTCGA TATCTCCTCT GTGGGCACAC CTCTCGCCCG AGCCAGCATC 
AAAAGTGCAA AGGTGGATGG GGCCTCCTAT TTCCGGCACA AAGAAAGGCT TCTGCGCATC 
TCTATCCGCC ACATGGTGAA ATCCCAGGTG TTTTACTGGA TTGTGTTGAG CCTTGTGGCT 
CTCAACACTG CCTGTGTGGC CATCGTCCAT CATAACCAGC CCCAGTGGCT CACCCATCTC 
CTCTACTATG CAGAATTTTT GTTTCTGGGA CTGTTCCTCT TGGAGATGTC CCTGAAGATG 
TATGGCATGG GACCTCGACT TTATTTTCAC TCTTCATTCA ACTGTTTTGA TTTTGGGGTC 
ACAGTGGGCA GCATCTTTGA AGTGGTCTGG GCAATCTTCA GACCTGGCAC ATCTTTTGGA 
ATCAGTGTCT TACGGGCTCT CCGGCTTCTA AGAATATTTA AAATAACCAA GTATTGGGCA 
TCTCTGCGAA ATTTGGTGGT CTCCTTGATG AGCTCTATGA AGTCTATCAT CAGTTTGCTC 
TTCCTCCTCT TCCTCTTCAT CGTTGTCTTT GCTCTCCTAG GAATGCAGCT GTTTGGAGGC 
AGGTTTAACT TTAATGATGG GACTCCTTCA GCAAACTTTG ATACCTTCCC TGCAGCCATC 
ATGACTGTAT TCCAGATCCT AACAGGTGAG GACTGGAATG AGGTGATGTA CAATGGGATC 
CGTTCCCAGG GTGGAGTTAG CTCAGGCATG TGGTCAGCTA TCTATTTCAT TGTGCTCACC 
TTGTTTGGAA ACTACACACT GCTGAATGTG TTCTTGGCCA TTGCTGTGGA CAATCTGGCC 
AATGCCCAAG AGCTGACCAA GGATGAACAA GAAGAAGAGG AGGCTTTTAA CCAGAAACAT 
GCATTGCAGA AGGCCAAGGA GGTCAGCCCG ATGTCTGCAC CCAACATGCC TTCTATCGAA 
AGAGACAGAA GGAGAAGACA CCACATGTCG ATGTGGGAGC CACGCAGCAG CCACCTGAGG 
GAGCGGAGGC GCCGGCACCA CATGTCGGTG TGGGAGCAGC GCACCAGCCA GCTGAGAAGG 
CACATGCAGA TGTCCAGCCA GGAGGCCCTC AATAAAGAGG AGGCCCCACC GATGAACCCC 
CTCAACCCCC TCAACCCGCT GAGCCCCCTC AACCCGCTCA ACGCCCACCC CAGCCTCTAC 
CGGAGACCTA GGCCTATTGA GGGCCTGGGC CTGGGCCTGG AGAAGTGTGA GGAGGAGCGC 
ATCATCAGCC GCGGGGGGTC CCTCAAGGGG GACGGAGGGG ACCTGTCGAG TGCCCTGGAC 
AACCAGAGGA GCCCTTTGTC CCTGGGCAAA CGGGAGCCAC CATGGCTGGC CAGGCCCTGT 
CATGGGAACT GTGATCCGAC CCAGCAGGAG GCTGGGGGAG GGGAGACCGT GGTGACCTTC 
GAGGACCGGG CCAGGCACAG GCAGAGCCAG CGGCGCAGCC GGCACCGTCG TGTCAGGACG 
GAAGGCAAGG AGCTCTCCTC CACCTCCCGG AGCAGGTCTG TCAGCCAGGA GCGGAGTCTG 
GATGAGGGGG TGCTCACCGA CGGGGAGAAG GAGCGAGAGC CCAGGGGCGG CCATGCAGGC 
AAAGAACCCA CCATCCAAGA AGAAGACAGA GGCCAAGACT TAAGGAGGAC GAACAGTCTG 
ATGGTGCCCA GAGGCTCTGG GCTGGCAGGA GCCCTTGATG AGGCCAACAC ACCCCTAGTC 
CTGCCCCACC ACGATCTGGA AGTGGGGAAG GACACGGTGC TGACGGAGCA GGAGCCGGAG 
GGCAGCAGCG AGCAGGCCCT GCTGGGGGAT GTGCAGTTGG ACATTGGCAG GGTCATCAGC 
CAGAGTGAGC CTGACCTCTC CTGCGTCACA GCCAACACAG ACAAGGCCAC CACCGACAGC 
ACCAGCGTCA CCGTCGCCAT CCCTGATGTG GGCCCCTTGA TGGACTCAAC TGTGATTCAC 
ATTAGCAACA AAACTGATGG AGAAGCCAGT CCCTTGAAGG AGGCTGAGAT CAAAGAGGAG 
GAGGAGGAAA TGGAGAAGAA GCAGCAGAAG AAGGAGAAGC GTGAGACGGG CAAAGCCATG 
GTGCCCCACA GCTCTATGTT CATCTTCAGC ACCACCAACC CGATTCGCAG GGCCTGCCAC 
TACATCGTGA ACCTGCGCTA CTTTGAGATG TGCATTCTCC TGGTGATCGC CGCCAGCAGC 
ATTGCCCTGG CAGCAGAGGA CCCCGTCCTG ACCAACTCGG AGCGCAACAA AGTGGGTTCA 
CGGGAGCCGT TTGCCAACCT CCGTAATGCC CTGGTCCTGA GGTATTTTGA CTATGTTTTC 
ACTGGCGTGT TCACCTTTGA GATGGTTATA AAGATGATAG ACCAGGGCTT GATCCTACAG 
GATGGGTCCT ACTTCCGAGA CCTGTGGAAC ATCCTGGACT TTGTGGTGGT GGTTGGTGCA 
TTGGTGGCCT TTGCTTTGGC AACAAACAAG GGGCGAGACA TCAAGACCAT CAAATCCCTG 
AGGGTGCTCC GAGTTCTGAG GCCACTGAAG ACCATCAAGC GCTTGCCCAA ACTCAAGGCT 
GTCTTTGACT GTGTGGTGAC CTCCTTGAAG AATGTCTTCA ACATACTCAT CGTCTATAAG 
CTCTTCATGT TCATCTTTGC TGTCATTGCG GTTCAGCTCT TCAAGGGAAA ATTCTTTTAT 
TGCACAGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTACGT AGATCATGAA 
AAAAACAAGA TGGAGGTGAA AGGCCGGGAA TGGAAGCGCC ATGAATTCCA CTACGACAAC 
ATTATCTGGG CCCTGCTGAC CCTCTTCACC GTCTCCACGG GGGAAGGATG GCCTCAAGTC 
TTACAGCATT CTGTCGATGT GACAGAGGAA GACCGAGGAC CAAGCCGCAG CAACCGCATG 
GAGATGTCCA TCTTCTATGT GGTCTACTTT GTGGTCTTCC CTTTCTTCTT TGTCAATATC 
TTTGTGGCTC TGATCATCAT CACCTTCCAG GAACAAGGGG ACAAGATGAT GGAGGAATGC 
AGCCTGGAGA AGAACGAGAG GGCGTGCATT GACTTCGCCA TCAGTGCCAA ACCTCTCACC 
CGCTACATGC CACAGAACAG GCACACCTTC CAGTACCGCG TCTGGCACTT CGTGGTGTCT 
CCGTCCTTTG AGTACACCAT CATGGCCATG ATCGCCCTGA ACACCGTCGT GCTGATGATG 
AAGTACTACT CTGCTCCCTG TACCTATGAA CTGGCCCTGA AGTACCTGAA TATTGCCTTC 
ACCATGGTGT TTTCCCTGGA ATGTGTCCTA AAGATCATCG CTTTTGGCTT CTTGAACTAC 
TTTCGAGACA CCTGGAACAT CTTTGACTTC ATCACTGTGA TTGGCAGTAT CACAGAAATC 
ATACTGACAG ACAGCAAGCT GGTAAACACC AGTGGTTTCA ACATGAGCTT TCTGAAGCTC 
TTCCGTGCCG CCCGCCTCAT CAAGCTTCTG CGGCAGGGCT ACACCATCCG TATTTTACTC 
TGGACCTTCG TGCAGTCCTT TAAGGCCCTC CCCTACGTCT GCCTTTTGAT TGCCATGCTT 
TTCTTCATCT ATGCCATCAT TGGGATGCAG GTATTTGGAA ACATAAAATT AGATGAGGAG 
AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GGTCCCTCAT GTTACTCTTC 
AGGAGTGCCA CGGGAGAGGC CTGGCAGGAG ATAATGCTGT CGTGCCTCGG GGAGAAAGGC 
TGCGAGCCCG ACACCACTGC GCCATCGGGG CAGAACGAGA ATGAGCGCTG TGGCACCGAT 
CTGGCCTATG TTTACTTTGT CTCCTTCATC TTCTTCTGCT CTTTCTTGAT GCTCAACTTG 
TTTGTGGCCG TCATCATGGA CAACTTCGAG TACCTGACTC GGGACTCCTC CATCTTAGGG 
CCCCACCACT TGGATGAGTT TGTCCGCGTC TGGGCAGAGT ACGACCGAGC TGCATGTGGC 
CGCATCCATT ATACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCACC GCTAGGCCTC 
GGCAAGAGAT GTCCCTCCAA AGTGGCATAT AAGAGGTTGG TCCTAATGAA TATGCCAGTA 
GCTGAGGACA TGACAGTCCA CTTCACCTCC ACACTGATGG CTCTGATCCG GACAGCCCTG 
GACATCAAAA TCGCCAAAGG TGGTGCAGAC AGGCAGCAGC TAGACTCGGA GCTGCAAAAG 
GAGACCCTGG CCATCTGGCC TCACCTGTCC CAGAAGATGC TAGACCTGCT TGTGCCCATG 
CCCAAAGCCT CTGACCTGAC TGTGGGCAAA ATCTATGCAG CAATGATGAT CATGGACTAC 
TATAAACAGA GTAAGGTGAA GAAACAGAGG CAGCAGCTGG AGGAACAGAA AAATGCACCC 
ATGTTCCAGC GAATGGAGCC CTCATCTCTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC 
CTGCCCTATC TCCAGCAGGA CCCCGTTTCA GGCCTGAGTG GCCGTAGTGG ATACCCTTCG 
ATGAGTCCAC TCTCCCCCCA GGAAATATTC CAATTGGCTT GTATGGATCC AGCCGATGAT 
GGACAGTTCC AGGAACATCA GTCTCTGGAG CCAGAGGTTA GTGAATTAAA AAGCGTGCAG 
TCCTCTAACC ATGGCATCTA CCTTCCTTCG GACACCCAGG AGCATACGGG ATCTGGGAGG 
GCATCTTCTA TGCCACGCCT GACTGTGGAT CCCCAGGTGG TGACAGATAC TAGCTCCATG 
AGACGTTCAT TTTCCACTAT TCGGGATAAG CGTTCAAATT CCTCCTGGTT GGAGGAATTC 
TCCATGGAGA GAAGCAGTGA AAACACCTAC AAGTCACGTC GTCGGAGTTA CCACTCCTCC 
CTGAGACTGT CAGCTCATCG CCTGAATTCT GACTCAGGCC ACAAGTCTGA CACCCACCGC 
TCAGGGGGTA GAGAGCGGGG ACGATCGAAA GAGCGAAAGC ATCTTCTCTC TCCTGATGTC 
TCCCGCTGCA ACTCAGAGGA GCGAGGGACC CAGGCCGACT GGGAGTCCCC AGAGCGCCAA 
CAGTCCAGGT CTCCCAGTGA GGCCAGGTCA CAGACCCCCA ACAGACAGGG CACGGGCTCC 
CTGAGCGAGA GCTCCATCCC CTCCATCTCT GACACCAGCA CCCCAAGACG AAGTCGCCGG 
CAGCTCCCAC CCGTCCCACC AAAGCCTCGG CCCCTCCTCT CCTACAGCTC CCTGATTCGA 
CACTCCGGGA GCATCTCCCC GCCGGCCGAC GGAAGCGAGA GGGGCTCCCC GCTGACCCCC 
CAGGCGCTGG AGAGCACGGA GGCCGGCCTG GCGGAGCCCT CGGGCCCCCG GCACGGGCAC 
GGGCAGGGGC ACGGGCAGGG GCACGGGCAG CAGAGCCAGC ACTGCTCCCC GCAGCGCTAC 
ATCTCCGAGC CCTACCTGGC CCTGCACGAA GACTCCCACG CGTCGGACTG TGGCGAGGAG 
GAGACGCTCA CCTTCGAGGC CGCCGTGGCC ACCAGCCTGG GCCGCTCCAA CACCATCGGC 
TCGGCCCCGC CGCTGCGGCA CAGCTGGCAG ATGCCCAACG GGCACTATCG GCGGCGGCGG 
CGCGGGGGGC CGGGGCCGGG CGTGATGTGC GGGGCGGTCA GCGACCTCCT GAGTGACACG 
GAAGAAGACG ACAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622448.1
CDS	86..7546
Translation	MEEQCSGMVPEMRGPSPGRRLPEPAPCPAAAGSPEPWAAGRRRHVLALPEEEAWRPQQCSLLEADASDEVGMLPASPRAAASGVDNFFPVQEGEGAGWEGGAALDAGAGPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFTNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLGLGLEKCEEERIISRGGSLKGDGGDLSSALDNQRSPLSLGKREPPWLARPCHGNCDPTQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKELSSTSRSRSVSQERSLDEGVLTDGEKEREPRGGHAGKEPTIQEEDRGQDLRRTNSLMVPRGSGLAGALDEANTPLVLPHHDLEVGKDTVLTEQEPEGSSEQALLGDVQLDIGRVISQSEPDLSCVTANTDKATTDSTSVTVAIPDVGPLMDSTVIHISNKTDGEASPLKEAEIKEEEEEMEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVGSREPFANLRNALVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALATNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEHQSLEPEVSELKSVQSSNHGIYLPSDTQEHTGSGRASSMPRLTVDPQVVTDTSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERQQSRSPSEARSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHSGSISPPADGSERGSPLTPQALESTEAGLAEPSGPRHGHGQGHGQGHGQQSQHCSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGVMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_005622391.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622391.2

ORF Insert Sequence:

ATGGAAGAGC AATGTTCTGG GATGGTGCCC GAGATGCGTG GACCGAGCCC AGGCCGCAGG 
CTGCCCGAGC CCGCCCCCTG CCCCGCCGCG GCCGGGAGCC CTGAGCCCTG GGCCGCGGGC 
CGCCGCCGCC ACGTCCTGGC CCTGCCTGAG GAGGAGGCAT GGAGGCCCCA GCAGTGCTCC 
CTGCTCGAGG CCGACGCCTC GGACGAGGTG GGCATGCTGC CGGCCTCCCC GCGCGCCGCC 
GCCTCCGGCG TCGACAACTT CTTCCCGGTG CAGGAGGGCG AGGGCGCGGG CTGGGAGGGC 
GGTGCGGCCC TGGACGCCGG CGCCGGCCCC TTCCTCCCGG TGAGCCCCGA GGTGATGAAG 
CGGCGGCGCG GGGGCCTCAT CGAGCAGCGC GACATCATCA AGGCCCACGA GGCGCACAAG 
ATGCAGAGCA CCCCGCAGGC GCGGCGCAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG 
GTGGTCGGCA GGCCGGGGTC GGGCGATGGA GACTCGGACC AGAGCAGGAA CCGGCAAGGA 
ACCCCGGTGC CGGCCTCGGG GCCGGCGGCC GCCTACAAGC AGTCGAAAGC GCAGAGGGCG 
CGGACTATGG CTTTGTACAA CCCCATTCCC GTCCGGCAGA ACTGTTTCAC CGTCAACAGA 
TCCCTGTTCA TCTTCGGAGA AGATAACATT GTCAGGAAAT ACGCCAAGAA GCTCATCGAC 
TGGCCGCCGT TTGAGTACAT GATTTTGGCA ACCATCATTG CCAACTGCAT TGTCTTGGCC 
CTGGAACAGC ATCTTCCTGA GGATGACAAG ACCCCGATGT CCCGCAGACT GGAGAAGACA 
GAGCCGTATT TCATTGGGAT CTTTTGCTTT GAAGCCGGTA TCAAAATTGT GGCCCTGGGG 
TTCATCTTTC ATAAGGGCTC GTACCTCCGC AATGGCTGGA ATGTCATGGA CTTCATCGTG 
GTCCTCAGTG GCATCCTGGC CACTGCGGGG ACTCACTTCA ACACACACGT GGACCTGAGG 
ACCCTCCGGG CTGTGCGTGT CCTGAGACCC CTGAAGCTCG TGTCAGGGAT TCCTAGCCTG 
CAGATCGTGT TGAAGTCTAT CATGAAGGCC ATGGTGCCTC TTCTGCAAAT TGGTCTCCTG 
CTCTTCTTTG CCATCTTGAT GTTTGCTATC ATTGGCTTAG AGTTCTACAG TGGGAAGTTG 
CACCGAGCAT GTTTCACGAA CAATTCAGGT ATTCTGGAAG GATTTGACCC CCCTCACCCA 
TGTGGTGTGC AGGGCTGCCC AGCTGGCTAC GAATGCCGGG ACTGGATTGG CCCCAATGAT 
GGGATCACCC AGTTTGATAA CATCCTTTTT GCTGTGCTGA CTGTCTTCCA ATGCATCACC 
ATGGAAGGAT GGACCACCGT GCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC 
TGGCTGTACT TCATCCCCCT CATCATCATT GGATCCTTCT TTGTTCTCAA CCTAGTTCTG 
GGAGTGCTTT CCGGGGAGTT TGCCAAAGAG AGAGAGAGAG TGGAGAACCG GAGAGCTTTC 
ATGAAACTGC GGCGGCAGCA GCAGATTGAG CGGGAGCTGA ACGGGTACCG CGCCTGGATA 
GACAAAGCAG AGGAAGTCAT GCTTGCTGAA GAAAATAAAA ATGCTGGGAC GTCAGCCTTA 
GAAGTGCTTC GAAGGGCAAC CATCAAAAGG AGCCGGACAG AGGCCATGAC TCGAGACTCC 
AGTGATGAAC ACTGTGTCGA TATCTCCTCT GTGGGCACAC CTCTCGCCCG AGCCAGCATC 
AAAAGTGCAA AGGTGGATGG GGCCTCCTAT TTCCGGCACA AAGAAAGGCT TCTGCGCATC 
TCTATCCGCC ACATGGTGAA ATCCCAGGTG TTTTACTGGA TTGTGTTGAG CCTTGTGGCT 
CTCAACACTG CCTGTGTGGC CATCGTCCAT CATAACCAGC CCCAGTGGCT CACCCATCTC 
CTCTACTATG CAGAATTTTT GTTTCTGGGA CTGTTCCTCT TGGAGATGTC CCTGAAGATG 
TATGGCATGG GACCTCGACT TTATTTTCAC TCTTCATTCA ACTGTTTTGA TTTTGGGGTC 
ACAGTGGGCA GCATCTTTGA AGTGGTCTGG GCAATCTTCA GACCTGGCAC ATCTTTTGGA 
ATCAGTGTCT TACGGGCTCT CCGGCTTCTA AGAATATTTA AAATAACCAA GTATTGGGCA 
TCTCTGCGAA ATTTGGTGGT CTCCTTGATG AGCTCTATGA AGTCTATCAT CAGTTTGCTC 
TTCCTCCTCT TCCTCTTCAT CGTTGTCTTT GCTCTCCTAG GAATGCAGCT GTTTGGAGGC 
AGGTTTAACT TTAATGATGG GACTCCTTCA GCAAACTTTG ATACCTTCCC TGCAGCCATC 
ATGACTGTAT TCCAGATCCT AACAGGTGAG GACTGGAATG AGGTGATGTA CAATGGGATC 
CGTTCCCAGG GTGGAGTTAG CTCAGGCATG TGGTCAGCTA TCTATTTCAT TGTGCTCACC 
TTGTTTGGAA ACTACACACT GCTGAATGTG TTCTTGGCCA TTGCTGTGGA CAATCTGGCC 
AATGCCCAAG AGCTGACCAA GGATGAACAA GAAGAAGAGG AGGCTTTTAA CCAGAAACAT 
GCATTGCAGA AGGCCAAGGA GGTCAGCCCG ATGTCTGCAC CCAACATGCC TTCTATCGAA 
AGAGACAGAA GGAGAAGACA CCACATGTCG ATGTGGGAGC CACGCAGCAG CCACCTGAGG 
GAGCGGAGGC GCCGGCACCA CATGTCGGTG TGGGAGCAGC GCACCAGCCA GCTGAGAAGG 
CACATGCAGA TGTCCAGCCA GGAGGCCCTC AATAAAGAGG AGGCCCCACC GATGAACCCC 
CTCAACCCCC TCAACCCGCT GAGCCCCCTC AACCCGCTCA ACGCCCACCC CAGCCTCTAC 
CGGAGACCTA GGCCTATTGA GGGCCTGGGC CTGGGCCTGG AGAAGTGTGA GGAGGAGCGC 
ATCATCAGCC GCGGGGGGTC CCTCAAGGGG GACGGAGGGG ACCTGTCGAG TGCCCTGGAC 
AACCAGAGGA GCCCTTTGTC CCTGGGCAAA CGGGAGCCAC CATGGCTGGC CAGGCCCTGT 
CATGGGAACT GTGATCCGAC CCAGCAGGAG GCTGGGGGAG GGGAGACCGT GGTGACCTTC 
GAGGACCGGG CCAGGCACAG GCAGAGCCAG CGGCGCAGCC GGCACCGTCG TGTCAGGACG 
GAAGGCAAGG AGCTCTCCTC CACCTCCCGG AGCAGGTCTG TCAGCCAGGA GCGGAGTCTG 
GATGAGGGGG TGCTCACCGA CGGGGAGAAG GAGCGAGAGC CCAGGGGCGG CCATGCAGGC 
AAAGAACCCA CCATCCAAGA AGAAGACAGA GGCCAAGACT TAAGGAGGAC GAACAGTCTG 
ATGGTGCCCA GAGGCTCTGG GCTGGCAGGA GCCCTTGATG AGGCCAACAC ACCCCTAGTC 
CTGCCCCACC ACGATCTGGA AGTGGGGAAG GACACGGTGC TGACGGAGCA GGAGCCGGAG 
GGCAGCAGCG AGCAGGCCCT GCTGGGGGAT GTGCAGTTGG ACATTGGCAG GGTCATCAGC 
CAGAGTGAGC CTGACCTCTC CTGCGTCACA GCCAACACAG ACAAGGCCAC CACCGACAGC 
ACCAGCGTCA CCGTCGCCAT CCCTGATGTG GGCCCCTTGA TGGACTCAAC TGTGATTCAC 
ATTAGCAACA AAACTGATGG AGAAGCCAGT CCCTTGAAGG AGGCTGAGAT CAAAGAGGAG 
GAGGAGGAAA TGGAGAAGAA GCAGCAGAAG AAGGAGAAGC GTGAGACGGG CAAAGCCATG 
GTGCCCCACA GCTCTATGTT CATCTTCAGC ACCACCAACC CGATTCGCAG GGCCTGCCAC 
TACATCGTGA ACCTGCGCTA CTTTGAGATG TGCATTCTCC TGGTGATCGC CGCCAGCAGC 
ATTGCCCTGG CAGCAGAGGA CCCCGTCCTG ACCAACTCGG AGCGCAACAA AGTGGGTTCA 
CGGGAGCCGT TTGCCAACCT CCGTAATGCC CTGGTCCTGA GGTATTTTGA CTATGTTTTC 
ACTGGCGTGT TCACCTTTGA GATGGTTATA AAGATGATAG ACCAGGGCTT GATCCTACAG 
GATGGGTCCT ACTTCCGAGA CCTGTGGAAC ATCCTGGACT TTGTGGTGGT GGTTGGTGCA 
TTGGTGGCCT TTGCTTTGGC AACAAACAAG GGGCGAGACA TCAAGACCAT CAAATCCCTG 
AGGGTGCTCC GAGTTCTGAG GCCACTGAAG ACCATCAAGC GCTTGCCCAA ACTCAAGGCT 
GTCTTTGACT GTGTGGTGAC CTCCTTGAAG AATGTCTTCA ACATACTCAT CGTCTATAAG 
CTCTTCATGT TCATCTTTGC TGTCATTGCG GTTCAGCTCT TCAAGGGAAA ATTCTTTTAT 
TGCACAGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTACGT AGATCATGAA 
AAAAACAAGA TGGAGGTGAA AGGCCGGGAA TGGAAGCGCC ATGAATTCCA CTACGACAAC 
ATTATCTGGG CCCTGCTGAC CCTCTTCACC GTCTCCACGG GGGAAGGATG GCCTCAAGTC 
TTACAGCATT CTGTCGATGT GACAGAGGAA GACCGAGGAC CAAGCCGCAG CAACCGCATG 
GAGATGTCCA TCTTCTATGT GGTCTACTTT GTGGTCTTCC CTTTCTTCTT TGTCAATATC 
TTTGTGGCTC TGATCATCAT CACCTTCCAG GAACAAGGGG ACAAGATGAT GGAGGAATGC 
AGCCTGGAGA AGAACGAGAG GGCGTGCATT GACTTCGCCA TCAGTGCCAA ACCTCTCACC 
CGCTACATGC CACAGAACAG GCACACCTTC CAGTACCGCG TCTGGCACTT CGTGGTGTCT 
CCGTCCTTTG AGTACACCAT CATGGCCATG ATCGCCCTGA ACACCGTCGT GCTGATGATG 
AAGTACTACT CTGCTCCCTG TACCTATGAA CTGGCCCTGA AGTACCTGAA TATTGCCTTC 
ACCATGGTGT TTTCCCTGGA ATGTGTCCTA AAGATCATCG CTTTTGGCTT CTTGAACTAC 
TTTCGAGACA CCTGGAACAT CTTTGACTTC ATCACTGTGA TTGGCAGTAT CACAGAAATC 
ATACTGACAG ACAGCAAGCT GGTAAACACC AGTGGTTTCA ACATGAGCTT TCTGAAGCTC 
TTCCGTGCCG CCCGCCTCAT CAAGCTTCTG CGGCAGGGCT ACACCATCCG TATTTTACTC 
TGGACCTTCG TGCAGTCCTT TAAGGCCCTC CCCTACGTCT GCCTTTTGAT TGCCATGCTT 
TTCTTCATCT ATGCCATCAT TGGGATGCAG GTATTTGGAA ACATAAAATT AGATGAGGAG 
AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GGTCCCTCAT GTTACTCTTC 
AGGAGTGCCA CGGGAGAGGC CTGGCAGGAG ATAATGCTGT CGTGCCTCGG GGAGAAAGGC 
TGCGAGCCCG ACACCACTGC GCCATCGGGG CAGAACGAGA ATGAGCGCTG TGGCACCGAT 
CTGGCCTATG TTTACTTTGT CTCCTTCATC TTCTTCTGCT CTTTCTTGAT GCTCAACTTG 
TTTGTGGCCG TCATCATGGA CAACTTCGAG TACCTGACTC GGGACTCCTC CATCTTAGGG 
CCCCACCACT TGGATGAGTT TGTCCGCGTC TGGGCAGAGT ACGACCGAGC TGCATGTGGC 
CGCATCCATT ATACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCACC GCTAGGCCTC 
GGCAAGAGAT GTCCCTCCAA AGTGGCATAT AAGAGGTTGG TCCTAATGAA TATGCCAGTA 
GCTGAGGACA TGACAGTCCA CTTCACCTCC ACACTGATGG CTCTGATCCG GACAGCCCTG 
GACATCAAAA TCGCCAAAGG TGGTGCAGAC AGGCAGCAGC TAGACTCGGA GCTGCAAAAG 
GAGACCCTGG CCATCTGGCC TCACCTGTCC CAGAAGATGC TAGACCTGCT TGTGCCCATG 
CCCAAAGCCT CTGACCTGAC TGTGGGCAAA ATCTATGCAG CAATGATGAT CATGGACTAC 
TATAAACAGA GTAAGGTGAA GAAACAGAGG CAGCAGCTGG AGGAACAGAA AAATGCACCC 
ATGTTCCAGC GAATGGAGCC CTCATCTCTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC 
CTGCCCTATC TCCAGCAGGA CCCCGTTTCA GGCCTGAGTG GCCGTAGTGG ATACCCTTCG 
ATGAGTCCAC TCTCCCCCCA GGAAATATTC CAATTGGCTT GTATGGATCC AGCCGATGAT 
GGACAGTTCC AGGAACATCA GTCTCTGGAG CCAGAGGTTA GTGAATTAAA AAGCGTGCAG 
TCCTCTAACC ATGGCATCTA CCTTCCTTCG GACACCCAGG AGCATACGGG ATCTGGGAGG 
GCATCTTCTA TGCCACGCCT GACTGTGGAT CCCCAGGTGG TGACAGATAC TAGCTCCATG 
AGACGTTCAT TTTCCACTAT TCGGGATAAG CGTTCAAATT CCTCCTGGTT GGAGGAATTC 
TCCATGGAGA GAAGCAGTGA AAACACCTAC AAGTCACGTC GTCGGAGTTA CCACTCCTCC 
CTGAGACTGT CAGCTCATCG CCTGAATTCT GACTCAGGCC ACAAGTCTGA CACCCACCGC 
TCAGGGGGTA GAGAGCGGGG ACGATCGAAA GAGCGAAAGC ATCTTCTCTC TCCTGATGTC 
TCCCGCTGCA ACTCAGAGGA GCGAGGGACC CAGGCCGACT GGGAGTCCCC AGAGCGCCAA 
CAGTCCAGGT CTCCCAGTGA GGCCAGGTCA CAGACCCCCA ACAGACAGGG CACGGGCTCC 
CTGAGCGAGA GCTCCATCCC CTCCATCTCT GACACCAGCA CCCCAAGACG AAGTCGCCGG 
CAGCTCCCAC CCGTCCCACC AAAGCCTCGG CCCCTCCTCT CCTACAGCTC CCTGATTCGA 
CACTCCGGGA GCATCTCCCC GCCGGCCGAC GGAAGCGAGA GGGGCTCCCC GCTGACCCCC 
CAGGCGCTGG AGAGCACGGA GGCCGGCCTG GCGGAGCCCT CGGGCCCCCG GCACGGGCAC 
GGGCAGGGGC ACGGGCAGGG GCACGGGCAG CAGAGCCAGC ACTGCTCCCC GCAGCGCTAC 
ATCTCCGAGC CCTACCTGGC CCTGCACGAA GACTCCCACG CGTCGGACTG TGGCGAGGAG 
GAGACGCTCA CCTTCGAGGC CGCCGTGGCC ACCAGCCTGG GCCGCTCCAA CACCATCGGC 
TCGGCCCCGC CGCTGCGGCA CAGCTGGCAG ATGCCCAACG GGCACTATCG GCGGCGGCGG 
CGCGGGGGGC CGGGGCCGGG CGTGATGTGC GGGGCGGTCA GCGACCTCCT GAGTGACACG 
GAAGAAGACG ACAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_005622392.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622449.1 Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe09114
Clone ID Related Accession (Same CDS sequence)	XM_005622392.2
Accession Version	XM_005622392.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005622392.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACCGAGCCCA GGCCGCAGGC TGCCCGAGCC CGCCCCCTGC 
CCCGCCGCGG CCGGGAGCCC TGAGCCCTGG GCCGCGGGCC GCCGCCGCCA CGTCCTGGCC 
CTGCCTGAGG AGGAGGCATG GAGGCCCCAG CAGTGCTCCC TGCTCGAGGC CGACGCCTCG 
GACGAGGTGG GCATGCTGCC GGCCTCCCCG CGCGCCGCCG CCTCCGGCGT CGACAACTTC 
TTCCCGGTGC AGGAGGGCGA GGGCGCGGGC TGGGAGGGCG GTGCGGCCCT GGACGCCGGC 
GCCGGCCCCT TCCTCCCGGT GAGCCCCGAG GTGATGAAGC GGCGGCGCGG GGGCCTCATC 
GAGCAGCGCG ACATCATCAA GGCCCACGAG GCGCACAAGA TGCAGAGCAC CCCGCAGGCG 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGGCAG GCCGGGGTCG 
GGCGATGGAG ACTCGGACCA GAGCAGGAAC CGGCAAGGAA CCCCGGTGCC GGCCTCGGGG 
CCGGCGGCCG CCTACAAGCA GTCGAAAGCG CAGAGGGCGC GGACTATGGC TTTGTACAAC 
CCCATTCCCG TCCGGCAGAA CTGTTTCACC GTCAACAGAT CCCTGTTCAT CTTCGGAGAA 
GATAACATTG TCAGGAAATA CGCCAAGAAG CTCATCGACT GGCCGCCGTT TGAGTACATG 
ATTTTGGCAA CCATCATTGC CAACTGCATT GTCTTGGCCC TGGAACAGCA TCTTCCTGAG 
GATGACAAGA CCCCGATGTC CCGCAGACTG GAGAAGACAG AGCCGTATTT CATTGGGATC 
TTTTGCTTTG AAGCCGGTAT CAAAATTGTG GCCCTGGGGT TCATCTTTCA TAAGGGCTCG 
TACCTCCGCA ATGGCTGGAA TGTCATGGAC TTCATCGTGG TCCTCAGTGG CATCCTGGCC 
ACTGCGGGGA CTCACTTCAA CACACACGTG GACCTGAGGA CCCTCCGGGC TGTGCGTGTC 
CTGAGACCCC TGAAGCTCGT GTCAGGGATT CCTAGCCTGC AGATCGTGTT GAAGTCTATC 
ATGAAGGCCA TGGTGCCTCT TCTGCAAATT GGTCTCCTGC TCTTCTTTGC CATCTTGATG 
TTTGCTATCA TTGGCTTAGA GTTCTACAGT GGGAAGTTGC ACCGAGCATG TTTCACGAAC 
AATTCAGGTA TTCTGGAAGG ATTTGACCCC CCTCACCCAT GTGGTGTGCA GGGCTGCCCA 
GCTGGCTACG AATGCCGGGA CTGGATTGGC CCCAATGATG GGATCACCCA GTTTGATAAC 
ATCCTTTTTG CTGTGCTGAC TGTCTTCCAA TGCATCACCA TGGAAGGATG GACCACCGTG 
CTGTACAATA CCAATGATGC CTTAGGAGCC ACCTGGAACT GGCTGTACTT CATCCCCCTC 
ATCATCATTG GATCCTTCTT TGTTCTCAAC CTAGTTCTGG GAGTGCTTTC CGGGGAGTTT 
GCCAAAGAGA GAGAGAGAGT GGAGAACCGG AGAGCTTTCA TGAAACTGCG GCGGCAGCAG 
CAGATTGAGC GGGAGCTGAA CGGGTACCGC GCCTGGATAG ACAAAGCAGA GGAAGTCATG 
CTTGCTGAAG AAAATAAAAA TGCTGGGACG TCAGCCTTAG AAGTGCTTCG AAGGGCAACC 
ATCAAAAGGA GCCGGACAGA GGCCATGACT CGAGACTCCA GTGATGAACA CTGTGTCGAT 
ATCTCCTCTG TGGGCACACC TCTCGCCCGA GCCAGCATCA AAAGTGCAAA GGTGGATGGG 
GCCTCCTATT TCCGGCACAA AGAAAGGCTT CTGCGCATCT CTATCCGCCA CATGGTGAAA 
TCCCAGGTGT TTTACTGGAT TGTGTTGAGC CTTGTGGCTC TCAACACTGC CTGTGTGGCC 
ATCGTCCATC ATAACCAGCC CCAGTGGCTC ACCCATCTCC TCTACTATGC AGAATTTTTG 
TTTCTGGGAC TGTTCCTCTT GGAGATGTCC CTGAAGATGT ATGGCATGGG ACCTCGACTT 
TATTTTCACT CTTCATTCAA CTGTTTTGAT TTTGGGGTCA CAGTGGGCAG CATCTTTGAA 
GTGGTCTGGG CAATCTTCAG ACCTGGCACA TCTTTTGGAA TCAGTGTCTT ACGGGCTCTC 
CGGCTTCTAA GAATATTTAA AATAACCAAG TATTGGGCAT CTCTGCGAAA TTTGGTGGTC 
TCCTTGATGA GCTCTATGAA GTCTATCATC AGTTTGCTCT TCCTCCTCTT CCTCTTCATC 
GTTGTCTTTG CTCTCCTAGG AATGCAGCTG TTTGGAGGCA GGTTTAACTT TAATGATGGG 
ACTCCTTCAG CAAACTTTGA TACCTTCCCT GCAGCCATCA TGACTGTATT CCAGATCCTA 
ACAGGTGAGG ACTGGAATGA GGTGATGTAC AATGGGATCC GTTCCCAGGG TGGAGTTAGC 
TCAGGCATGT GGTCAGCTAT CTATTTCATT GTGCTCACCT TGTTTGGAAA CTACACACTG 
CTGAATGTGT TCTTGGCCAT TGCTGTGGAC AATCTGGCCA ATGCCCAAGA GCTGACCAAG 
GATGAACAAG AAGAAGAGGA GGCTTTTAAC CAGAAACATG CATTGCAGAA GGCCAAGGAG 
GTCAGCCCGA TGTCTGCACC CAACATGCCT TCTATCGAAA GAGACAGAAG GAGAAGACAC 
CACATGTCGA TGTGGGAGCC ACGCAGCAGC CACCTGAGGG AGCGGAGGCG CCGGCACCAC 
ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGAGAAGGC ACATGCAGAT GTCCAGCCAG 
GAGGCCCTCA ATAAAGAGGA GGCCCCACCG ATGAACCCCC TCAACCCCCT CAACCCGCTG 
AGCCCCCTCA ACCCGCTCAA CGCCCACCCC AGCCTCTACC GGAGACCTAG GCCTATTGAG 
GGCCTGGGCC TGGGCCTGGA GAAGTGTGAG GAGGAGCGCA TCATCAGCCG CGGGGGGTCC 
CTCAAGGGGG ACGGAGGGGA CCTGTCGAGT GCCCTGGACA ACCAGAGGAG CCCTTTGTCC 
CTGGGCAAAC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGGAACTG TGATCCGACC 
CAGCAGGAGG CTGGGGGAGG GGAGACCGTG GTGACCTTCG AGGACCGGGC CAGGCACAGG 
CAGAGCCAGC GGCGCAGCCG GCACCGTCGT GTCAGGACGG AAGGCAAGGA GCTCTCCTCC 
ACCTCCCGGA GCAGGTCTGT CAGCCAGGAG CGGAGTCTGG ATGAGGGGGT GCTCACCGAC 
GGGGAGAAGG AGCGAGAGCC CAGGGGCGGC CATGCAGGCA AAGAACCCAC CATCCAAGAA 
GAAGACAGAG GCCAAGACTT AAGGAGGACG AACAGTCTGA TGGTGCCCAG AGGCTCTGGG 
CTGGCAGGAG CCCTTGATGA GGCCAACACA CCCCTAGTCC TGCCCCACCA CGATCTGGAA 
GTGGGGAAGG ACACGGTGCT GACGGAGCAG GAGCCGGAGG GCAGCAGCGA GCAGGCCCTG 
CTGGGGGATG TGCAGTTGGA CATTGGCAGG GTCATCAGCC AGAGTGAGCC TGACCTCTCC 
TGCGTCACAG CCAACACAGA CAAGGCCACC ACCGACAGCA CCAGCGTCAC CGTCGCCATC 
CCTGATGTGG GCCCCTTGAT GGACTCAACT GTGATTCACA TTAGCAACAA AACTGATGGA 
GAAGCCAGTC CCTTGAAGGA GGCTGAGATC AAAGAGGAGG AGGAGGAAAT GGAGAAGAAG 
CAGCAGAAGA AGGAGAAGCG TGAGACGGGC AAAGCCATGG TGCCCCACAG CTCTATGTTC 
ATCTTCAGCA CCACCAACCC GATTCGCAGG GCCTGCCACT ACATCGTGAA CCTGCGCTAC 
TTTGAGATGT GCATTCTCCT GGTGATCGCC GCCAGCAGCA TTGCCCTGGC AGCAGAGGAC 
CCCGTCCTGA CCAACTCGGA GCGCAACAAA GTGGGTTCAC GGGAGCCGTT TGCCAACCTC 
CGTAATGCCC TGGTCCTGAG GTATTTTGAC TATGTTTTCA CTGGCGTGTT CACCTTTGAG 
ATGGTTATAA AGATGATAGA CCAGGGCTTG ATCCTACAGG ATGGGTCCTA CTTCCGAGAC 
CTGTGGAACA TCCTGGACTT TGTGGTGGTG GTTGGTGCAT TGGTGGCCTT TGCTTTGGCG 
AACGCTTTGG GAACAAACAA GGGGCGAGAC ATCAAGACCA TCAAATCCCT GAGGGTGCTC 
CGAGTTCTGA GGCCACTGAA GACCATCAAG CGCTTGCCCA AACTCAAGGC TGTCTTTGAC 
TGTGTGGTGA CCTCCTTGAA GAATGTCTTC AACATACTCA TCGTCTATAA GCTCTTCATG 
TTCATCTTTG CTGTCATTGC GGTTCAGCTC TTCAAGGGAA AATTCTTTTA TTGCACAGAC 
AGTTCCAAGG ACACAGAGAA GGAGTGCATA GGCAACTACG TAGATCATGA AAAAAACAAG 
ATGGAGGTGA AAGGCCGGGA ATGGAAGCGC CATGAATTCC ACTACGACAA CATTATCTGG 
GCCCTGCTGA CCCTCTTCAC CGTCTCCACG GGGGAAGGAT GGCCTCAAGT CTTACAGCAT 
TCTGTCGATG TGACAGAGGA AGACCGAGGA CCAAGCCGCA GCAACCGCAT GGAGATGTCC 
ATCTTCTATG TGGTCTACTT TGTGGTCTTC CCTTTCTTCT TTGTCAATAT CTTTGTGGCT 
CTGATCATCA TCACCTTCCA GGAACAAGGG GACAAGATGA TGGAGGAATG CAGCCTGGAG 
AAGAACGAGA GGGCGTGCAT TGACTTCGCC ATCAGTGCCA AACCTCTCAC CCGCTACATG 
CCACAGAACA GGCACACCTT CCAGTACCGC GTCTGGCACT TCGTGGTGTC TCCGTCCTTT 
GAGTACACCA TCATGGCCAT GATCGCCCTG AACACCGTCG TGCTGATGAT GAAGTACTAC 
TCTGCTCCCT GTACCTATGA ACTGGCCCTG AAGTACCTGA ATATTGCCTT CACCATGGTG 
TTTTCCCTGG AATGTGTCCT AAAGATCATC GCTTTTGGCT TCTTGAACTA CTTTCGAGAC 
ACCTGGAACA TCTTTGACTT CATCACTGTG ATTGGCAGTA TCACAGAAAT CATACTGACA 
GACAGCAAGC TGGTAAACAC CAGTGGTTTC AACATGAGCT TTCTGAAGCT CTTCCGTGCC 
GCCCGCCTCA TCAAGCTTCT GCGGCAGGGC TACACCATCC GTATTTTACT CTGGACCTTC 
GTGCAGTCCT TTAAGGCCCT CCCCTACGTC TGCCTTTTGA TTGCCATGCT TTTCTTCATC 
TATGCCATCA TTGGGATGCA GGTATTTGGA AACATAAAAT TAGATGAGGA GAGTCACATC 
AACCGGCACA ACAACTTCCG GAGTTTCTTT GGGTCCCTCA TGTTACTCTT CAGGAGTGCC 
ACGGGAGAGG CCTGGCAGGA GATAATGCTG TCGTGCCTCG GGGAGAAAGG CTGCGAGCCC 
GACACCACTG CGCCATCGGG GCAGAACGAG AATGAGCGCT GTGGCACCGA TCTGGCCTAT 
GTTTACTTTG TCTCCTTCAT CTTCTTCTGC TCTTTCTTGA TGCTCAACTT GTTTGTGGCC 
GTCATCATGG ACAACTTCGA GTACCTGACT CGGGACTCCT CCATCTTAGG GCCCCACCAC 
TTGGATGAGT TTGTCCGCGT CTGGGCAGAG TACGACCGAG CTGCATGTGG CCGCATCCAT 
TATACTGAGA TGTATGAAAT GCTGACTCTC ATGTCACCAC CGCTAGGCCT CGGCAAGAGA 
TGTCCCTCCA AAGTGGCATA TAAGAGGTTG GTCCTAATGA ATATGCCAGT AGCTGAGGAC 
ATGACAGTCC ACTTCACCTC CACACTGATG GCTCTGATCC GGACAGCCCT GGACATCAAA 
ATCGCCAAAG GTGGTGCAGA CAGGCAGCAG CTAGACTCGG AGCTGCAAAA GGAGACCCTG 
GCCATCTGGC CTCACCTGTC CCAGAAGATG CTAGACCTGC TTGTGCCCAT GCCCAAAGCC 
TCTGACCTGA CTGTGGGCAA AATCTATGCA GCAATGATGA TCATGGACTA CTATAAACAG 
AGTAAGGTGA AGAAACAGAG GCAGCAGCTG GAGGAACAGA AAAATGCACC CATGTTCCAG 
CGAATGGAGC CCTCATCTCT GCCTCAGGAG ATCATTGCTA ATGCCAAAGC CCTGCCCTAT 
CTCCAGCAGG ACCCCGTTTC AGGCCTGAGT GGCCGTAGTG GATACCCTTC GATGAGTCCA 
CTCTCCCCCC AGGAAATATT CCAATTGGCT TGTATGGATC CAGCCGATGA TGGACAGTTC 
CAGGAACATC AGTCTCTGGA GCCAGAGGTT AGTGAATTAA AAAGCGTGCA GTCCTCTAAC 
CATGGCATCT ACCTTCCTTC GGACACCCAG GAGCATACGG GATCTGGGAG GGCATCTTCT 
ATGCCACGCC TGACTGTGGA TCCCCAGGTG GTGACAGATA CTAGCTCCAT GAGACGTTCA 
TTTTCCACTA TTCGGGATAA GCGTTCAAAT TCCTCCTGGT TGGAGGAATT CTCCATGGAG 
AGAAGCAGTG AAAACACCTA CAAGTCACGT CGTCGGAGTT ACCACTCCTC CCTGAGACTG 
TCAGCTCATC GCCTGAATTC TGACTCAGGC CACAAGTCTG ACACCCACCG CTCAGGGGGT 
AGAGAGCGGG GACGATCGAA AGAGCGAAAG CATCTTCTCT CTCCTGATGT CTCCCGCTGC 
AACTCAGAGG AGCGAGGGAC CCAGGCCGAC TGGGAGTCCC CAGAGCGCCA ACAGTCCAGG 
TCTCCCAGTG AGGCCAGGTC ACAGACCCCC AACAGACAGG GCACGGGCTC CCTGAGCGAG 
AGCTCCATCC CCTCCATCTC TGACACCAGC ACCCCAAGAC GAAGTCGCCG GCAGCTCCCA 
CCCGTCCCAC CAAAGCCTCG GCCCCTCCTC TCCTACAGCT CCCTGATTCG ACACTCCGGG 
AGCATCTCCC CGCCGGCCGA CGGAAGCGAG AGGGGCTCCC CGCTGACCCC CCAGGCGCTG 
GAGAGCACGG AGGCCGGCCT GGCGGAGCCC TCGGGCCCCC GGCACGGGCA CGGGCAGGGG 
CACGGGCAGG GGCACGGGCA GCAGAGCCAG CACTGCTCCC CGCAGCGCTA CATCTCCGAG 
CCCTACCTGG CCCTGCACGA AGACTCCCAC GCGTCGGACT GTGGCGAGGA GGAGACGCTC 
ACCTTCGAGG CCGCCGTGGC CACCAGCCTG GGCCGCTCCA ACACCATCGG CTCGGCCCCG 
CCGCTGCGGC ACAGCTGGCA GATGCCCAAC GGGCACTATC GGCGGCGGCG GCGCGGGGGG 
CCGGGGCCGG GCGTGATGTG CGGGGCGGTC AGCGACCTCC TGAGTGACAC GGAAGAAGAC 
GACAAGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622449.1
CDS	111..7562
Translation	MVPEMRGPSPGRRLPEPAPCPAAAGSPEPWAAGRRRHVLALPEEEAWRPQQCSLLEADASDEVGMLPASPRAAASGVDNFFPVQEGEGAGWEGGAALDAGAGPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFTNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLGLGLEKCEEERIISRGGSLKGDGGDLSSALDNQRSPLSLGKREPPWLARPCHGNCDPTQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKELSSTSRSRSVSQERSLDEGVLTDGEKEREPRGGHAGKEPTIQEEDRGQDLRRTNSLMVPRGSGLAGALDEANTPLVLPHHDLEVGKDTVLTEQEPEGSSEQALLGDVQLDIGRVISQSEPDLSCVTANTDKATTDSTSVTVAIPDVGPLMDSTVIHISNKTDGEASPLKEAEIKEEEEEMEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVGSREPFANLRNALVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEHQSLEPEVSELKSVQSSNHGIYLPSDTQEHTGSGRASSMPRLTVDPQVVTDTSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERQQSRSPSEARSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHSGSISPPADGSERGSPLTPQALESTEAGLAEPSGPRHGHGQGHGQGHGQQSQHCSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGVMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_005622392.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622392.2

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACCGAGCCCA GGCCGCAGGC TGCCCGAGCC CGCCCCCTGC 
CCCGCCGCGG CCGGGAGCCC TGAGCCCTGG GCCGCGGGCC GCCGCCGCCA CGTCCTGGCC 
CTGCCTGAGG AGGAGGCATG GAGGCCCCAG CAGTGCTCCC TGCTCGAGGC CGACGCCTCG 
GACGAGGTGG GCATGCTGCC GGCCTCCCCG CGCGCCGCCG CCTCCGGCGT CGACAACTTC 
TTCCCGGTGC AGGAGGGCGA GGGCGCGGGC TGGGAGGGCG GTGCGGCCCT GGACGCCGGC 
GCCGGCCCCT TCCTCCCGGT GAGCCCCGAG GTGATGAAGC GGCGGCGCGG GGGCCTCATC 
GAGCAGCGCG ACATCATCAA GGCCCACGAG GCGCACAAGA TGCAGAGCAC CCCGCAGGCG 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGGCAG GCCGGGGTCG 
GGCGATGGAG ACTCGGACCA GAGCAGGAAC CGGCAAGGAA CCCCGGTGCC GGCCTCGGGG 
CCGGCGGCCG CCTACAAGCA GTCGAAAGCG CAGAGGGCGC GGACTATGGC TTTGTACAAC 
CCCATTCCCG TCCGGCAGAA CTGTTTCACC GTCAACAGAT CCCTGTTCAT CTTCGGAGAA 
GATAACATTG TCAGGAAATA CGCCAAGAAG CTCATCGACT GGCCGCCGTT TGAGTACATG 
ATTTTGGCAA CCATCATTGC CAACTGCATT GTCTTGGCCC TGGAACAGCA TCTTCCTGAG 
GATGACAAGA CCCCGATGTC CCGCAGACTG GAGAAGACAG AGCCGTATTT CATTGGGATC 
TTTTGCTTTG AAGCCGGTAT CAAAATTGTG GCCCTGGGGT TCATCTTTCA TAAGGGCTCG 
TACCTCCGCA ATGGCTGGAA TGTCATGGAC TTCATCGTGG TCCTCAGTGG CATCCTGGCC 
ACTGCGGGGA CTCACTTCAA CACACACGTG GACCTGAGGA CCCTCCGGGC TGTGCGTGTC 
CTGAGACCCC TGAAGCTCGT GTCAGGGATT CCTAGCCTGC AGATCGTGTT GAAGTCTATC 
ATGAAGGCCA TGGTGCCTCT TCTGCAAATT GGTCTCCTGC TCTTCTTTGC CATCTTGATG 
TTTGCTATCA TTGGCTTAGA GTTCTACAGT GGGAAGTTGC ACCGAGCATG TTTCACGAAC 
AATTCAGGTA TTCTGGAAGG ATTTGACCCC CCTCACCCAT GTGGTGTGCA GGGCTGCCCA 
GCTGGCTACG AATGCCGGGA CTGGATTGGC CCCAATGATG GGATCACCCA GTTTGATAAC 
ATCCTTTTTG CTGTGCTGAC TGTCTTCCAA TGCATCACCA TGGAAGGATG GACCACCGTG 
CTGTACAATA CCAATGATGC CTTAGGAGCC ACCTGGAACT GGCTGTACTT CATCCCCCTC 
ATCATCATTG GATCCTTCTT TGTTCTCAAC CTAGTTCTGG GAGTGCTTTC CGGGGAGTTT 
GCCAAAGAGA GAGAGAGAGT GGAGAACCGG AGAGCTTTCA TGAAACTGCG GCGGCAGCAG 
CAGATTGAGC GGGAGCTGAA CGGGTACCGC GCCTGGATAG ACAAAGCAGA GGAAGTCATG 
CTTGCTGAAG AAAATAAAAA TGCTGGGACG TCAGCCTTAG AAGTGCTTCG AAGGGCAACC 
ATCAAAAGGA GCCGGACAGA GGCCATGACT CGAGACTCCA GTGATGAACA CTGTGTCGAT 
ATCTCCTCTG TGGGCACACC TCTCGCCCGA GCCAGCATCA AAAGTGCAAA GGTGGATGGG 
GCCTCCTATT TCCGGCACAA AGAAAGGCTT CTGCGCATCT CTATCCGCCA CATGGTGAAA 
TCCCAGGTGT TTTACTGGAT TGTGTTGAGC CTTGTGGCTC TCAACACTGC CTGTGTGGCC 
ATCGTCCATC ATAACCAGCC CCAGTGGCTC ACCCATCTCC TCTACTATGC AGAATTTTTG 
TTTCTGGGAC TGTTCCTCTT GGAGATGTCC CTGAAGATGT ATGGCATGGG ACCTCGACTT 
TATTTTCACT CTTCATTCAA CTGTTTTGAT TTTGGGGTCA CAGTGGGCAG CATCTTTGAA 
GTGGTCTGGG CAATCTTCAG ACCTGGCACA TCTTTTGGAA TCAGTGTCTT ACGGGCTCTC 
CGGCTTCTAA GAATATTTAA AATAACCAAG TATTGGGCAT CTCTGCGAAA TTTGGTGGTC 
TCCTTGATGA GCTCTATGAA GTCTATCATC AGTTTGCTCT TCCTCCTCTT CCTCTTCATC 
GTTGTCTTTG CTCTCCTAGG AATGCAGCTG TTTGGAGGCA GGTTTAACTT TAATGATGGG 
ACTCCTTCAG CAAACTTTGA TACCTTCCCT GCAGCCATCA TGACTGTATT CCAGATCCTA 
ACAGGTGAGG ACTGGAATGA GGTGATGTAC AATGGGATCC GTTCCCAGGG TGGAGTTAGC 
TCAGGCATGT GGTCAGCTAT CTATTTCATT GTGCTCACCT TGTTTGGAAA CTACACACTG 
CTGAATGTGT TCTTGGCCAT TGCTGTGGAC AATCTGGCCA ATGCCCAAGA GCTGACCAAG 
GATGAACAAG AAGAAGAGGA GGCTTTTAAC CAGAAACATG CATTGCAGAA GGCCAAGGAG 
GTCAGCCCGA TGTCTGCACC CAACATGCCT TCTATCGAAA GAGACAGAAG GAGAAGACAC 
CACATGTCGA TGTGGGAGCC ACGCAGCAGC CACCTGAGGG AGCGGAGGCG CCGGCACCAC 
ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGAGAAGGC ACATGCAGAT GTCCAGCCAG 
GAGGCCCTCA ATAAAGAGGA GGCCCCACCG ATGAACCCCC TCAACCCCCT CAACCCGCTG 
AGCCCCCTCA ACCCGCTCAA CGCCCACCCC AGCCTCTACC GGAGACCTAG GCCTATTGAG 
GGCCTGGGCC TGGGCCTGGA GAAGTGTGAG GAGGAGCGCA TCATCAGCCG CGGGGGGTCC 
CTCAAGGGGG ACGGAGGGGA CCTGTCGAGT GCCCTGGACA ACCAGAGGAG CCCTTTGTCC 
CTGGGCAAAC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGGAACTG TGATCCGACC 
CAGCAGGAGG CTGGGGGAGG GGAGACCGTG GTGACCTTCG AGGACCGGGC CAGGCACAGG 
CAGAGCCAGC GGCGCAGCCG GCACCGTCGT GTCAGGACGG AAGGCAAGGA GCTCTCCTCC 
ACCTCCCGGA GCAGGTCTGT CAGCCAGGAG CGGAGTCTGG ATGAGGGGGT GCTCACCGAC 
GGGGAGAAGG AGCGAGAGCC CAGGGGCGGC CATGCAGGCA AAGAACCCAC CATCCAAGAA 
GAAGACAGAG GCCAAGACTT AAGGAGGACG AACAGTCTGA TGGTGCCCAG AGGCTCTGGG 
CTGGCAGGAG CCCTTGATGA GGCCAACACA CCCCTAGTCC TGCCCCACCA CGATCTGGAA 
GTGGGGAAGG ACACGGTGCT GACGGAGCAG GAGCCGGAGG GCAGCAGCGA GCAGGCCCTG 
CTGGGGGATG TGCAGTTGGA CATTGGCAGG GTCATCAGCC AGAGTGAGCC TGACCTCTCC 
TGCGTCACAG CCAACACAGA CAAGGCCACC ACCGACAGCA CCAGCGTCAC CGTCGCCATC 
CCTGATGTGG GCCCCTTGAT GGACTCAACT GTGATTCACA TTAGCAACAA AACTGATGGA 
GAAGCCAGTC CCTTGAAGGA GGCTGAGATC AAAGAGGAGG AGGAGGAAAT GGAGAAGAAG 
CAGCAGAAGA AGGAGAAGCG TGAGACGGGC AAAGCCATGG TGCCCCACAG CTCTATGTTC 
ATCTTCAGCA CCACCAACCC GATTCGCAGG GCCTGCCACT ACATCGTGAA CCTGCGCTAC 
TTTGAGATGT GCATTCTCCT GGTGATCGCC GCCAGCAGCA TTGCCCTGGC AGCAGAGGAC 
CCCGTCCTGA CCAACTCGGA GCGCAACAAA GTGGGTTCAC GGGAGCCGTT TGCCAACCTC 
CGTAATGCCC TGGTCCTGAG GTATTTTGAC TATGTTTTCA CTGGCGTGTT CACCTTTGAG 
ATGGTTATAA AGATGATAGA CCAGGGCTTG ATCCTACAGG ATGGGTCCTA CTTCCGAGAC 
CTGTGGAACA TCCTGGACTT TGTGGTGGTG GTTGGTGCAT TGGTGGCCTT TGCTTTGGCG 
AACGCTTTGG GAACAAACAA GGGGCGAGAC ATCAAGACCA TCAAATCCCT GAGGGTGCTC 
CGAGTTCTGA GGCCACTGAA GACCATCAAG CGCTTGCCCA AACTCAAGGC TGTCTTTGAC 
TGTGTGGTGA CCTCCTTGAA GAATGTCTTC AACATACTCA TCGTCTATAA GCTCTTCATG 
TTCATCTTTG CTGTCATTGC GGTTCAGCTC TTCAAGGGAA AATTCTTTTA TTGCACAGAC 
AGTTCCAAGG ACACAGAGAA GGAGTGCATA GGCAACTACG TAGATCATGA AAAAAACAAG 
ATGGAGGTGA AAGGCCGGGA ATGGAAGCGC CATGAATTCC ACTACGACAA CATTATCTGG 
GCCCTGCTGA CCCTCTTCAC CGTCTCCACG GGGGAAGGAT GGCCTCAAGT CTTACAGCAT 
TCTGTCGATG TGACAGAGGA AGACCGAGGA CCAAGCCGCA GCAACCGCAT GGAGATGTCC 
ATCTTCTATG TGGTCTACTT TGTGGTCTTC CCTTTCTTCT TTGTCAATAT CTTTGTGGCT 
CTGATCATCA TCACCTTCCA GGAACAAGGG GACAAGATGA TGGAGGAATG CAGCCTGGAG 
AAGAACGAGA GGGCGTGCAT TGACTTCGCC ATCAGTGCCA AACCTCTCAC CCGCTACATG 
CCACAGAACA GGCACACCTT CCAGTACCGC GTCTGGCACT TCGTGGTGTC TCCGTCCTTT 
GAGTACACCA TCATGGCCAT GATCGCCCTG AACACCGTCG TGCTGATGAT GAAGTACTAC 
TCTGCTCCCT GTACCTATGA ACTGGCCCTG AAGTACCTGA ATATTGCCTT CACCATGGTG 
TTTTCCCTGG AATGTGTCCT AAAGATCATC GCTTTTGGCT TCTTGAACTA CTTTCGAGAC 
ACCTGGAACA TCTTTGACTT CATCACTGTG ATTGGCAGTA TCACAGAAAT CATACTGACA 
GACAGCAAGC TGGTAAACAC CAGTGGTTTC AACATGAGCT TTCTGAAGCT CTTCCGTGCC 
GCCCGCCTCA TCAAGCTTCT GCGGCAGGGC TACACCATCC GTATTTTACT CTGGACCTTC 
GTGCAGTCCT TTAAGGCCCT CCCCTACGTC TGCCTTTTGA TTGCCATGCT TTTCTTCATC 
TATGCCATCA TTGGGATGCA GGTATTTGGA AACATAAAAT TAGATGAGGA GAGTCACATC 
AACCGGCACA ACAACTTCCG GAGTTTCTTT GGGTCCCTCA TGTTACTCTT CAGGAGTGCC 
ACGGGAGAGG CCTGGCAGGA GATAATGCTG TCGTGCCTCG GGGAGAAAGG CTGCGAGCCC 
GACACCACTG CGCCATCGGG GCAGAACGAG AATGAGCGCT GTGGCACCGA TCTGGCCTAT 
GTTTACTTTG TCTCCTTCAT CTTCTTCTGC TCTTTCTTGA TGCTCAACTT GTTTGTGGCC 
GTCATCATGG ACAACTTCGA GTACCTGACT CGGGACTCCT CCATCTTAGG GCCCCACCAC 
TTGGATGAGT TTGTCCGCGT CTGGGCAGAG TACGACCGAG CTGCATGTGG CCGCATCCAT 
TATACTGAGA TGTATGAAAT GCTGACTCTC ATGTCACCAC CGCTAGGCCT CGGCAAGAGA 
TGTCCCTCCA AAGTGGCATA TAAGAGGTTG GTCCTAATGA ATATGCCAGT AGCTGAGGAC 
ATGACAGTCC ACTTCACCTC CACACTGATG GCTCTGATCC GGACAGCCCT GGACATCAAA 
ATCGCCAAAG GTGGTGCAGA CAGGCAGCAG CTAGACTCGG AGCTGCAAAA GGAGACCCTG 
GCCATCTGGC CTCACCTGTC CCAGAAGATG CTAGACCTGC TTGTGCCCAT GCCCAAAGCC 
TCTGACCTGA CTGTGGGCAA AATCTATGCA GCAATGATGA TCATGGACTA CTATAAACAG 
AGTAAGGTGA AGAAACAGAG GCAGCAGCTG GAGGAACAGA AAAATGCACC CATGTTCCAG 
CGAATGGAGC CCTCATCTCT GCCTCAGGAG ATCATTGCTA ATGCCAAAGC CCTGCCCTAT 
CTCCAGCAGG ACCCCGTTTC AGGCCTGAGT GGCCGTAGTG GATACCCTTC GATGAGTCCA 
CTCTCCCCCC AGGAAATATT CCAATTGGCT TGTATGGATC CAGCCGATGA TGGACAGTTC 
CAGGAACATC AGTCTCTGGA GCCAGAGGTT AGTGAATTAA AAAGCGTGCA GTCCTCTAAC 
CATGGCATCT ACCTTCCTTC GGACACCCAG GAGCATACGG GATCTGGGAG GGCATCTTCT 
ATGCCACGCC TGACTGTGGA TCCCCAGGTG GTGACAGATA CTAGCTCCAT GAGACGTTCA 
TTTTCCACTA TTCGGGATAA GCGTTCAAAT TCCTCCTGGT TGGAGGAATT CTCCATGGAG 
AGAAGCAGTG AAAACACCTA CAAGTCACGT CGTCGGAGTT ACCACTCCTC CCTGAGACTG 
TCAGCTCATC GCCTGAATTC TGACTCAGGC CACAAGTCTG ACACCCACCG CTCAGGGGGT 
AGAGAGCGGG GACGATCGAA AGAGCGAAAG CATCTTCTCT CTCCTGATGT CTCCCGCTGC 
AACTCAGAGG AGCGAGGGAC CCAGGCCGAC TGGGAGTCCC CAGAGCGCCA ACAGTCCAGG 
TCTCCCAGTG AGGCCAGGTC ACAGACCCCC AACAGACAGG GCACGGGCTC CCTGAGCGAG 
AGCTCCATCC CCTCCATCTC TGACACCAGC ACCCCAAGAC GAAGTCGCCG GCAGCTCCCA 
CCCGTCCCAC CAAAGCCTCG GCCCCTCCTC TCCTACAGCT CCCTGATTCG ACACTCCGGG 
AGCATCTCCC CGCCGGCCGA CGGAAGCGAG AGGGGCTCCC CGCTGACCCC CCAGGCGCTG 
GAGAGCACGG AGGCCGGCCT GGCGGAGCCC TCGGGCCCCC GGCACGGGCA CGGGCAGGGG 
CACGGGCAGG GGCACGGGCA GCAGAGCCAG CACTGCTCCC CGCAGCGCTA CATCTCCGAG 
CCCTACCTGG CCCTGCACGA AGACTCCCAC GCGTCGGACT GTGGCGAGGA GGAGACGCTC 
ACCTTCGAGG CCGCCGTGGC CACCAGCCTG GGCCGCTCCA ACACCATCGG CTCGGCCCCG 
CCGCTGCGGC ACAGCTGGCA GATGCCCAAC GGGCACTATC GGCGGCGGCG GCGCGGGGGG 
CCGGGGCCGG GCGTGATGTG CGGGGCGGTC AGCGACCTCC TGAGTGACAC GGAAGAAGAC 
GACAAGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_547425.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_547425.4 Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe09115
Clone ID Related Accession (Same CDS sequence)	XM_547425.5
Accession Version	XM_547425.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_547425.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGC AATGTTCTGG GATGGTGCCC GAGATGCGTG GACCGAGCCC AGGCCGCAGG 
CTGCCCGAGC CCGCCCCCTG CCCCGCCGCG GCCGGGAGCC CTGAGCCCTG GGCCGCGGGC 
CGCCGCCGCC ACGTCCTGGC CCTGCCTGAG GAGGAGGCAT GGAGGCCCCA GCAGTGCTCC 
CTGCTCGAGG CCGACGCCTC GGACGAGGTG GGCATGCTGC CGGCCTCCCC GCGCGCCGCC 
GCCTCCGGCG TCGACAACTT CTTCCCGGTG CAGGAGGGCG AGGGCGCGGG CTGGGAGGGC 
GGTGCGGCCC TGGACGCCGG CGCCGGCCCC TTCCTCCCGG TGAGCCCCGA GGTGATGAAG 
CGGCGGCGCG GGGGCCTCAT CGAGCAGCGC GACATCATCA AGGCCCACGA GGCGCACAAG 
ATGCAGAGCA CCCCGCAGGC GCGGCGCAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG 
GTGGTCGGCA GGCCGGGGTC GGGCGATGGA GACTCGGACC AGAGCAGGAA CCGGCAAGGA 
ACCCCGGTGC CGGCCTCGGG GCCGGCGGCC GCCTACAAGC AGTCGAAAGC GCAGAGGGCG 
CGGACTATGG CTTTGTACAA CCCCATTCCC GTCCGGCAGA ACTGTTTCAC CGTCAACAGA 
TCCCTGTTCA TCTTCGGAGA AGATAACATT GTCAGGAAAT ACGCCAAGAA GCTCATCGAC 
TGGCCGCCGT TTGAGTACAT GATTTTGGCA ACCATCATTG CCAACTGCAT TGTCTTGGCC 
CTGGAACAGC ATCTTCCTGA GGATGACAAG ACCCCGATGT CCCGCAGACT GGAGAAGACA 
GAGCCGTATT TCATTGGGAT CTTTTGCTTT GAAGCCGGTA TCAAAATTGT GGCCCTGGGG 
TTCATCTTTC ATAAGGGCTC GTACCTCCGC AATGGCTGGA ATGTCATGGA CTTCATCGTG 
GTCCTCAGTG GCATCCTGGC CACTGCGGGG ACTCACTTCA ACACACACGT GGACCTGAGG 
ACCCTCCGGG CTGTGCGTGT CCTGAGACCC CTGAAGCTCG TGTCAGGGAT TCCTAGCCTG 
CAGATCGTGT TGAAGTCTAT CATGAAGGCC ATGGTGCCTC TTCTGCAAAT TGGTCTCCTG 
CTCTTCTTTG CCATCTTGAT GTTTGCTATC ATTGGCTTAG AGTTCTACAG TGGGAAGTTG 
CACCGAGCAT GTTTCACGAA CAATTCAGGT ATTCTGGAAG GATTTGACCC CCCTCACCCA 
TGTGGTGTGC AGGGCTGCCC AGCTGGCTAC GAATGCCGGG ACTGGATTGG CCCCAATGAT 
GGGATCACCC AGTTTGATAA CATCCTTTTT GCTGTGCTGA CTGTCTTCCA ATGCATCACC 
ATGGAAGGAT GGACCACCGT GCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC 
TGGCTGTACT TCATCCCCCT CATCATCATT GGATCCTTCT TTGTTCTCAA CCTAGTTCTG 
GGAGTGCTTT CCGGGGAGTT TGCCAAAGAG AGAGAGAGAG TGGAGAACCG GAGAGCTTTC 
ATGAAACTGC GGCGGCAGCA GCAGATTGAG CGGGAGCTGA ACGGGTACCG CGCCTGGATA 
GACAAAGCAG AGGAAGTCAT GCTTGCTGAA GAAAATAAAA ATGCTGGGAC GTCAGCCTTA 
GAAGTGCTTC GAAGGGCAAC CATCAAAAGG AGCCGGACAG AGGCCATGAC TCGAGACTCC 
AGTGATGAAC ACTGTGTCGA TATCTCCTCT GTGGGCACAC CTCTCGCCCG AGCCAGCATC 
AAAAGTGCAA AGGTGGATGG GGCCTCCTAT TTCCGGCACA AAGAAAGGCT TCTGCGCATC 
TCTATCCGCC ACATGGTGAA ATCCCAGGTG TTTTACTGGA TTGTGTTGAG CCTTGTGGCT 
CTCAACACTG CCTGTGTGGC CATCGTCCAT CATAACCAGC CCCAGTGGCT CACCCATCTC 
CTCTACTATG CAGAATTTTT GTTTCTGGGA CTGTTCCTCT TGGAGATGTC CCTGAAGATG 
TATGGCATGG GACCTCGACT TTATTTTCAC TCTTCATTCA ACTGTTTTGA TTTTGGGGTC 
ACAGTGGGCA GCATCTTTGA AGTGGTCTGG GCAATCTTCA GACCTGGCAC ATCTTTTGGA 
ATCAGTGTCT TACGGGCTCT CCGGCTTCTA AGAATATTTA AAATAACCAA GTATTGGGCA 
TCTCTGCGAA ATTTGGTGGT CTCCTTGATG AGCTCTATGA AGTCTATCAT CAGTTTGCTC 
TTCCTCCTCT TCCTCTTCAT CGTTGTCTTT GCTCTCCTAG GAATGCAGCT GTTTGGAGGC 
AGGTTTAACT TTAATGATGG GACTCCTTCA GCAAACTTTG ATACCTTCCC TGCAGCCATC 
ATGACTGTAT TCCAGATCCT AACAGGTGAG GACTGGAATG AGGTGATGTA CAATGGGATC 
CGTTCCCAGG GTGGAGTTAG CTCAGGCATG TGGTCAGCTA TCTATTTCAT TGTGCTCACC 
TTGTTTGGAA ACTACACACT GCTGAATGTG TTCTTGGCCA TTGCTGTGGA CAATCTGGCC 
AATGCCCAAG AGCTGACCAA GGATGAACAA GAAGAAGAGG AGGCTTTTAA CCAGAAACAT 
GCATTGCAGA AGGCCAAGGA GGTCAGCCCG ATGTCTGCAC CCAACATGCC TTCTATCGAA 
AGAGACAGAA GGAGAAGACA CCACATGTCG ATGTGGGAGC CACGCAGCAG CCACCTGAGG 
GAGCGGAGGC GCCGGCACCA CATGTCGGTG TGGGAGCAGC GCACCAGCCA GCTGAGAAGG 
CACATGCAGA TGTCCAGCCA GGAGGCCCTC AATAAAGAGG AGGCCCCACC GATGAACCCC 
CTCAACCCCC TCAACCCGCT GAGCCCCCTC AACCCGCTCA ACGCCCACCC CAGCCTCTAC 
CGGAGACCTA GGCCTATTGA GGGCCTGGGC CTGGGCCTGG AGAAGTGTGA GGAGGAGCGC 
ATCATCAGCC GCGGGGGGTC CCTCAAGGGG GACGGAGGGG ACCTGTCGAG TGCCCTGGAC 
AACCAGAGGA GCCCTTTGTC CCTGGGCAAA CGGGAGCCAC CATGGCTGGC CAGGCCCTGT 
CATGGGAACT GTGATCCGAC CCAGCAGGAG GCTGGGGGAG GGGAGACCGT GGTGACCTTC 
GAGGACCGGG CCAGGCACAG GCAGAGCCAG CGGCGCAGCC GGCACCGTCG TGTCAGGACG 
GAAGGCAAGG AGCTCTCCTC CACCTCCCGG AGCAGGTCTG TCAGCCAGGA GCGGAGTCTG 
GATGAGGGGG TGCTCACCGA CGGGGAGAAG GAGCGAGAGC CCAGGGGCGG CCATGCAGGC 
AAAGAACCCA CCATCCAAGA AGAAGACAGA GGCCAAGACT TAAGGAGGAC GAACAGTCTG 
ATGGTGCCCA GAGGCTCTGG GCTGGCAGGA GCCCTTGATG AGGCCAACAC ACCCCTAGTC 
CTGCCCCACC ACGATCTGGA AGTGGGGAAG GACACGGTGC TGACGGAGCA GGAGCCGGAG 
GGCAGCAGCG AGCAGGCCCT GCTGGGGGAT GTGCAGTTGG ACATTGGCAG GGTCATCAGC 
CAGAGTGAGC CTGACCTCTC CTGCGTCACA GCCAACACAG ACAAGGCCAC CACCGACAGC 
ACCAGCGTCA CCGTCGCCAT CCCTGATGTG GGCCCCTTGA TGGACTCAAC TGTGATTCAC 
ATTAGCAACA AAACTGATGG AGAAGCCAGT CCCTTGAAGG AGGCTGAGAT CAAAGAGGAG 
GAGGAGGAAA TGGAGAAGAA GCAGCAGAAG AAGGAGAAGC GTGAGACGGG CAAAGCCATG 
GTGCCCCACA GCTCTATGTT CATCTTCAGC ACCACCAACC CGATTCGCAG GGCCTGCCAC 
TACATCGTGA ACCTGCGCTA CTTTGAGATG TGCATTCTCC TGGTGATCGC CGCCAGCAGC 
ATTGCCCTGG CAGCAGAGGA CCCCGTCCTG ACCAACTCGG AGCGCAACAA AGTCCTGAGG 
TATTTTGACT ATGTTTTCAC TGGCGTGTTC ACCTTTGAGA TGGTTATAAA GATGATAGAC 
CAGGGCTTGA TCCTACAGGA TGGGTCCTAC TTCCGAGACC TGTGGAACAT CCTGGACTTT 
GTGGTGGTGG TTGGTGCATT GGTGGCCTTT GCTTTGGCGA ACGCTTTGGG AACAAACAAG 
GGGCGAGACA TCAAGACCAT CAAATCCCTG AGGGTGCTCC GAGTTCTGAG GCCACTGAAG 
ACCATCAAGC GCTTGCCCAA ACTCAAGGCT GTCTTTGACT GTGTGGTGAC CTCCTTGAAG 
AATGTCTTCA ACATACTCAT CGTCTATAAG CTCTTCATGT TCATCTTTGC TGTCATTGCG 
GTTCAGCTCT TCAAGGGAAA ATTCTTTTAT TGCACAGACA GTTCCAAGGA CACAGAGAAG 
GAGTGCATAG GCAACTACGT AGATCATGAA AAAAACAAGA TGGAGGTGAA AGGCCGGGAA 
TGGAAGCGCC ATGAATTCCA CTACGACAAC ATTATCTGGG CCCTGCTGAC CCTCTTCACC 
GTCTCCACGG GGGAAGGATG GCCTCAAGTC TTACAGCATT CTGTCGATGT GACAGAGGAA 
GACCGAGGAC CAAGCCGCAG CAACCGCATG GAGATGTCCA TCTTCTATGT GGTCTACTTT 
GTGGTCTTCC CTTTCTTCTT TGTCAATATC TTTGTGGCTC TGATCATCAT CACCTTCCAG 
GAACAAGGGG ACAAGATGAT GGAGGAATGC AGCCTGGAGA AGAACGAGAG GGCGTGCATT 
GACTTCGCCA TCAGTGCCAA ACCTCTCACC CGCTACATGC CACAGAACAG GCACACCTTC 
CAGTACCGCG TCTGGCACTT CGTGGTGTCT CCGTCCTTTG AGTACACCAT CATGGCCATG 
ATCGCCCTGA ACACCGTCGT GCTGATGATG AAGTACTACT CTGCTCCCTG TACCTATGAA 
CTGGCCCTGA AGTACCTGAA TATTGCCTTC ACCATGGTGT TTTCCCTGGA ATGTGTCCTA 
AAGATCATCG CTTTTGGCTT CTTGAACTAC TTTCGAGACA CCTGGAACAT CTTTGACTTC 
ATCACTGTGA TTGGCAGTAT CACAGAAATC ATACTGACAG ACAGCAAGCT GGTAAACACC 
AGTGGTTTCA ACATGAGCTT TCTGAAGCTC TTCCGTGCCG CCCGCCTCAT CAAGCTTCTG 
CGGCAGGGCT ACACCATCCG TATTTTACTC TGGACCTTCG TGCAGTCCTT TAAGGCCCTC 
CCCTACGTCT GCCTTTTGAT TGCCATGCTT TTCTTCATCT ATGCCATCAT TGGGATGCAG 
GTATTTGGAA ACATAAAATT AGATGAGGAG AGTCACATCA ACCGGCACAA CAACTTCCGG 
AGTTTCTTTG GGTCCCTCAT GTTACTCTTC AGGAGTGCCA CGGGAGAGGC CTGGCAGGAG 
ATAATGCTGT CGTGCCTCGG GGAGAAAGGC TGCGAGCCCG ACACCACTGC GCCATCGGGG 
CAGAACGAGA ATGAGCGCTG TGGCACCGAT CTGGCCTATG TTTACTTTGT CTCCTTCATC 
TTCTTCTGCT CTTTCTTGAT GCTCAACTTG TTTGTGGCCG TCATCATGGA CAACTTCGAG 
TACCTGACTC GGGACTCCTC CATCTTAGGG CCCCACCACT TGGATGAGTT TGTCCGCGTC 
TGGGCAGAGT ACGACCGAGC TGCATGTGGC CGCATCCATT ATACTGAGAT GTATGAAATG 
CTGACTCTCA TGTCACCACC GCTAGGCCTC GGCAAGAGAT GTCCCTCCAA AGTGGCATAT 
AAGAGGTTGG TCCTAATGAA TATGCCAGTA GCTGAGGACA TGACAGTCCA CTTCACCTCC 
ACACTGATGG CTCTGATCCG GACAGCCCTG GACATCAAAA TCGCCAAAGG TGGTGCAGAC 
AGGCAGCAGC TAGACTCGGA GCTGCAAAAG GAGACCCTGG CCATCTGGCC TCACCTGTCC 
CAGAAGATGC TAGACCTGCT TGTGCCCATG CCCAAAGCCT CTGACCTGAC TGTGGGCAAA 
ATCTATGCAG CAATGATGAT CATGGACTAC TATAAACAGA GTAAGGTGAA GAAACAGAGG 
CAGCAGCTGG AGGAACAGAA AAATGCACCC ATGTTCCAGC GAATGGAGCC CTCATCTCTG 
CCTCAGGAGA TCATTGCTAA TGCCAAAGCC CTGCCCTATC TCCAGCAGGA CCCCGTTTCA 
GGCCTGAGTG GCCGTAGTGG ATACCCTTCG ATGAGTCCAC TCTCCCCCCA GGAAATATTC 
CAATTGGCTT GTATGGATCC AGCCGATGAT GGACAGTTCC AGGAACATCA GTCTCTGGAG 
CCAGAGGTTA GTGAATTAAA AAGCGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTTCG 
GACACCCAGG AGCATACGGG ATCTGGGAGG GCATCTTCTA TGCCACGCCT GACTGTGGAT 
CCCCAGGTGG TGACAGATAC TAGCTCCATG AGACGTTCAT TTTCCACTAT TCGGGATAAG 
CGTTCAAATT CCTCCTGGTT GGAGGAATTC TCCATGGAGA GAAGCAGTGA AAACACCTAC 
AAGTCACGTC GTCGGAGTTA CCACTCCTCC CTGAGACTGT CAGCTCATCG CCTGAATTCT 
GACTCAGGCC ACAAGTCTGA CACCCACCGC TCAGGGGGTA GAGAGCGGGG ACGATCGAAA 
GAGCGAAAGC ATCTTCTCTC TCCTGATGTC TCCCGCTGCA ACTCAGAGGA GCGAGGGACC 
CAGGCCGACT GGGAGTCCCC AGAGCGCCAA CAGTCCAGGT CTCCCAGTGA GGCCAGGTCA 
CAGACCCCCA ACAGACAGGG CACGGGCTCC CTGAGCGAGA GCTCCATCCC CTCCATCTCT 
GACACCAGCA CCCCAAGACG AAGTCGCCGG CAGCTCCCAC CCGTCCCACC AAAGCCTCGG 
CCCCTCCTCT CCTACAGCTC CCTGATTCGA CACTCCGGGA GCATCTCCCC GCCGGCCGAC 
GGAAGCGAGA GGGGCTCCCC GCTGACCCCC CAGGCGCTGG AGAGCACGGA GGCCGGCCTG 
GCGGAGCCCT CGGGCCCCCG GCACGGGCAC GGGCAGGGGC ACGGGCAGGG GCACGGGCAG 
CAGAGCCAGC ACTGCTCCCC GCAGCGCTAC ATCTCCGAGC CCTACCTGGC CCTGCACGAA 
GACTCCCACG CGTCGGACTG TGGCGAGGAG GAGACGCTCA CCTTCGAGGC CGCCGTGGCC 
ACCAGCCTGG GCCGCTCCAA CACCATCGGC TCGGCCCCGC CGCTGCGGCA CAGCTGGCAG 
ATGCCCAACG GGCACTATCG GCGGCGGCGG CGCGGGGGGC CGGGGCCGGG CGTGATGTGC 
GGGGCGGTCA GCGACCTCCT GAGTGACACG GAAGAAGACG ACAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_547425.4
CDS	85..7515
Translation	MEEQCSGMVPEMRGPSPGRRLPEPAPCPAAAGSPEPWAAGRRRHVLALPEEEAWRPQQCSLLEADASDEVGMLPASPRAAASGVDNFFPVQEGEGAGWEGGAALDAGAGPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFTNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLGLGLEKCEEERIISRGGSLKGDGGDLSSALDNQRSPLSLGKREPPWLARPCHGNCDPTQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKELSSTSRSRSVSQERSLDEGVLTDGEKEREPRGGHAGKEPTIQEEDRGQDLRRTNSLMVPRGSGLAGALDEANTPLVLPHHDLEVGKDTVLTEQEPEGSSEQALLGDVQLDIGRVISQSEPDLSCVTANTDKATTDSTSVTVAIPDVGPLMDSTVIHISNKTDGEASPLKEAEIKEEEEEMEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEHQSLEPEVSELKSVQSSNHGIYLPSDTQEHTGSGRASSMPRLTVDPQVVTDTSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERQQSRSPSEARSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHSGSISPPADGSERGSPLTPQALESTEAGLAEPSGPRHGHGQGHGQGHGQQSQHCSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGVMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_547425.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_547425.5

ORF Insert Sequence:

ATGGAAGAGC AATGTTCTGG GATGGTGCCC GAGATGCGTG GACCGAGCCC AGGCCGCAGG 
CTGCCCGAGC CCGCCCCCTG CCCCGCCGCG GCCGGGAGCC CTGAGCCCTG GGCCGCGGGC 
CGCCGCCGCC ACGTCCTGGC CCTGCCTGAG GAGGAGGCAT GGAGGCCCCA GCAGTGCTCC 
CTGCTCGAGG CCGACGCCTC GGACGAGGTG GGCATGCTGC CGGCCTCCCC GCGCGCCGCC 
GCCTCCGGCG TCGACAACTT CTTCCCGGTG CAGGAGGGCG AGGGCGCGGG CTGGGAGGGC 
GGTGCGGCCC TGGACGCCGG CGCCGGCCCC TTCCTCCCGG TGAGCCCCGA GGTGATGAAG 
CGGCGGCGCG GGGGCCTCAT CGAGCAGCGC GACATCATCA AGGCCCACGA GGCGCACAAG 
ATGCAGAGCA CCCCGCAGGC GCGGCGCAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG 
GTGGTCGGCA GGCCGGGGTC GGGCGATGGA GACTCGGACC AGAGCAGGAA CCGGCAAGGA 
ACCCCGGTGC CGGCCTCGGG GCCGGCGGCC GCCTACAAGC AGTCGAAAGC GCAGAGGGCG 
CGGACTATGG CTTTGTACAA CCCCATTCCC GTCCGGCAGA ACTGTTTCAC CGTCAACAGA 
TCCCTGTTCA TCTTCGGAGA AGATAACATT GTCAGGAAAT ACGCCAAGAA GCTCATCGAC 
TGGCCGCCGT TTGAGTACAT GATTTTGGCA ACCATCATTG CCAACTGCAT TGTCTTGGCC 
CTGGAACAGC ATCTTCCTGA GGATGACAAG ACCCCGATGT CCCGCAGACT GGAGAAGACA 
GAGCCGTATT TCATTGGGAT CTTTTGCTTT GAAGCCGGTA TCAAAATTGT GGCCCTGGGG 
TTCATCTTTC ATAAGGGCTC GTACCTCCGC AATGGCTGGA ATGTCATGGA CTTCATCGTG 
GTCCTCAGTG GCATCCTGGC CACTGCGGGG ACTCACTTCA ACACACACGT GGACCTGAGG 
ACCCTCCGGG CTGTGCGTGT CCTGAGACCC CTGAAGCTCG TGTCAGGGAT TCCTAGCCTG 
CAGATCGTGT TGAAGTCTAT CATGAAGGCC ATGGTGCCTC TTCTGCAAAT TGGTCTCCTG 
CTCTTCTTTG CCATCTTGAT GTTTGCTATC ATTGGCTTAG AGTTCTACAG TGGGAAGTTG 
CACCGAGCAT GTTTCACGAA CAATTCAGGT ATTCTGGAAG GATTTGACCC CCCTCACCCA 
TGTGGTGTGC AGGGCTGCCC AGCTGGCTAC GAATGCCGGG ACTGGATTGG CCCCAATGAT 
GGGATCACCC AGTTTGATAA CATCCTTTTT GCTGTGCTGA CTGTCTTCCA ATGCATCACC 
ATGGAAGGAT GGACCACCGT GCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC 
TGGCTGTACT TCATCCCCCT CATCATCATT GGATCCTTCT TTGTTCTCAA CCTAGTTCTG 
GGAGTGCTTT CCGGGGAGTT TGCCAAAGAG AGAGAGAGAG TGGAGAACCG GAGAGCTTTC 
ATGAAACTGC GGCGGCAGCA GCAGATTGAG CGGGAGCTGA ACGGGTACCG CGCCTGGATA 
GACAAAGCAG AGGAAGTCAT GCTTGCTGAA GAAAATAAAA ATGCTGGGAC GTCAGCCTTA 
GAAGTGCTTC GAAGGGCAAC CATCAAAAGG AGCCGGACAG AGGCCATGAC TCGAGACTCC 
AGTGATGAAC ACTGTGTCGA TATCTCCTCT GTGGGCACAC CTCTCGCCCG AGCCAGCATC 
AAAAGTGCAA AGGTGGATGG GGCCTCCTAT TTCCGGCACA AAGAAAGGCT TCTGCGCATC 
TCTATCCGCC ACATGGTGAA ATCCCAGGTG TTTTACTGGA TTGTGTTGAG CCTTGTGGCT 
CTCAACACTG CCTGTGTGGC CATCGTCCAT CATAACCAGC CCCAGTGGCT CACCCATCTC 
CTCTACTATG CAGAATTTTT GTTTCTGGGA CTGTTCCTCT TGGAGATGTC CCTGAAGATG 
TATGGCATGG GACCTCGACT TTATTTTCAC TCTTCATTCA ACTGTTTTGA TTTTGGGGTC 
ACAGTGGGCA GCATCTTTGA AGTGGTCTGG GCAATCTTCA GACCTGGCAC ATCTTTTGGA 
ATCAGTGTCT TACGGGCTCT CCGGCTTCTA AGAATATTTA AAATAACCAA GTATTGGGCA 
TCTCTGCGAA ATTTGGTGGT CTCCTTGATG AGCTCTATGA AGTCTATCAT CAGTTTGCTC 
TTCCTCCTCT TCCTCTTCAT CGTTGTCTTT GCTCTCCTAG GAATGCAGCT GTTTGGAGGC 
AGGTTTAACT TTAATGATGG GACTCCTTCA GCAAACTTTG ATACCTTCCC TGCAGCCATC 
ATGACTGTAT TCCAGATCCT AACAGGTGAG GACTGGAATG AGGTGATGTA CAATGGGATC 
CGTTCCCAGG GTGGAGTTAG CTCAGGCATG TGGTCAGCTA TCTATTTCAT TGTGCTCACC 
TTGTTTGGAA ACTACACACT GCTGAATGTG TTCTTGGCCA TTGCTGTGGA CAATCTGGCC 
AATGCCCAAG AGCTGACCAA GGATGAACAA GAAGAAGAGG AGGCTTTTAA CCAGAAACAT 
GCATTGCAGA AGGCCAAGGA GGTCAGCCCG ATGTCTGCAC CCAACATGCC TTCTATCGAA 
AGAGACAGAA GGAGAAGACA CCACATGTCG ATGTGGGAGC CACGCAGCAG CCACCTGAGG 
GAGCGGAGGC GCCGGCACCA CATGTCGGTG TGGGAGCAGC GCACCAGCCA GCTGAGAAGG 
CACATGCAGA TGTCCAGCCA GGAGGCCCTC AATAAAGAGG AGGCCCCACC GATGAACCCC 
CTCAACCCCC TCAACCCGCT GAGCCCCCTC AACCCGCTCA ACGCCCACCC CAGCCTCTAC 
CGGAGACCTA GGCCTATTGA GGGCCTGGGC CTGGGCCTGG AGAAGTGTGA GGAGGAGCGC 
ATCATCAGCC GCGGGGGGTC CCTCAAGGGG GACGGAGGGG ACCTGTCGAG TGCCCTGGAC 
AACCAGAGGA GCCCTTTGTC CCTGGGCAAA CGGGAGCCAC CATGGCTGGC CAGGCCCTGT 
CATGGGAACT GTGATCCGAC CCAGCAGGAG GCTGGGGGAG GGGAGACCGT GGTGACCTTC 
GAGGACCGGG CCAGGCACAG GCAGAGCCAG CGGCGCAGCC GGCACCGTCG TGTCAGGACG 
GAAGGCAAGG AGCTCTCCTC CACCTCCCGG AGCAGGTCTG TCAGCCAGGA GCGGAGTCTG 
GATGAGGGGG TGCTCACCGA CGGGGAGAAG GAGCGAGAGC CCAGGGGCGG CCATGCAGGC 
AAAGAACCCA CCATCCAAGA AGAAGACAGA GGCCAAGACT TAAGGAGGAC GAACAGTCTG 
ATGGTGCCCA GAGGCTCTGG GCTGGCAGGA GCCCTTGATG AGGCCAACAC ACCCCTAGTC 
CTGCCCCACC ACGATCTGGA AGTGGGGAAG GACACGGTGC TGACGGAGCA GGAGCCGGAG 
GGCAGCAGCG AGCAGGCCCT GCTGGGGGAT GTGCAGTTGG ACATTGGCAG GGTCATCAGC 
CAGAGTGAGC CTGACCTCTC CTGCGTCACA GCCAACACAG ACAAGGCCAC CACCGACAGC 
ACCAGCGTCA CCGTCGCCAT CCCTGATGTG GGCCCCTTGA TGGACTCAAC TGTGATTCAC 
ATTAGCAACA AAACTGATGG AGAAGCCAGT CCCTTGAAGG AGGCTGAGAT CAAAGAGGAG 
GAGGAGGAAA TGGAGAAGAA GCAGCAGAAG AAGGAGAAGC GTGAGACGGG CAAAGCCATG 
GTGCCCCACA GCTCTATGTT CATCTTCAGC ACCACCAACC CGATTCGCAG GGCCTGCCAC 
TACATCGTGA ACCTGCGCTA CTTTGAGATG TGCATTCTCC TGGTGATCGC CGCCAGCAGC 
ATTGCCCTGG CAGCAGAGGA CCCCGTCCTG ACCAACTCGG AGCGCAACAA AGTCCTGAGG 
TATTTTGACT ATGTTTTCAC TGGCGTGTTC ACCTTTGAGA TGGTTATAAA GATGATAGAC 
CAGGGCTTGA TCCTACAGGA TGGGTCCTAC TTCCGAGACC TGTGGAACAT CCTGGACTTT 
GTGGTGGTGG TTGGTGCATT GGTGGCCTTT GCTTTGGCGA ACGCTTTGGG AACAAACAAG 
GGGCGAGACA TCAAGACCAT CAAATCCCTG AGGGTGCTCC GAGTTCTGAG GCCACTGAAG 
ACCATCAAGC GCTTGCCCAA ACTCAAGGCT GTCTTTGACT GTGTGGTGAC CTCCTTGAAG 
AATGTCTTCA ACATACTCAT CGTCTATAAG CTCTTCATGT TCATCTTTGC TGTCATTGCG 
GTTCAGCTCT TCAAGGGAAA ATTCTTTTAT TGCACAGACA GTTCCAAGGA CACAGAGAAG 
GAGTGCATAG GCAACTACGT AGATCATGAA AAAAACAAGA TGGAGGTGAA AGGCCGGGAA 
TGGAAGCGCC ATGAATTCCA CTACGACAAC ATTATCTGGG CCCTGCTGAC CCTCTTCACC 
GTCTCCACGG GGGAAGGATG GCCTCAAGTC TTACAGCATT CTGTCGATGT GACAGAGGAA 
GACCGAGGAC CAAGCCGCAG CAACCGCATG GAGATGTCCA TCTTCTATGT GGTCTACTTT 
GTGGTCTTCC CTTTCTTCTT TGTCAATATC TTTGTGGCTC TGATCATCAT CACCTTCCAG 
GAACAAGGGG ACAAGATGAT GGAGGAATGC AGCCTGGAGA AGAACGAGAG GGCGTGCATT 
GACTTCGCCA TCAGTGCCAA ACCTCTCACC CGCTACATGC CACAGAACAG GCACACCTTC 
CAGTACCGCG TCTGGCACTT CGTGGTGTCT CCGTCCTTTG AGTACACCAT CATGGCCATG 
ATCGCCCTGA ACACCGTCGT GCTGATGATG AAGTACTACT CTGCTCCCTG TACCTATGAA 
CTGGCCCTGA AGTACCTGAA TATTGCCTTC ACCATGGTGT TTTCCCTGGA ATGTGTCCTA 
AAGATCATCG CTTTTGGCTT CTTGAACTAC TTTCGAGACA CCTGGAACAT CTTTGACTTC 
ATCACTGTGA TTGGCAGTAT CACAGAAATC ATACTGACAG ACAGCAAGCT GGTAAACACC 
AGTGGTTTCA ACATGAGCTT TCTGAAGCTC TTCCGTGCCG CCCGCCTCAT CAAGCTTCTG 
CGGCAGGGCT ACACCATCCG TATTTTACTC TGGACCTTCG TGCAGTCCTT TAAGGCCCTC 
CCCTACGTCT GCCTTTTGAT TGCCATGCTT TTCTTCATCT ATGCCATCAT TGGGATGCAG 
GTATTTGGAA ACATAAAATT AGATGAGGAG AGTCACATCA ACCGGCACAA CAACTTCCGG 
AGTTTCTTTG GGTCCCTCAT GTTACTCTTC AGGAGTGCCA CGGGAGAGGC CTGGCAGGAG 
ATAATGCTGT CGTGCCTCGG GGAGAAAGGC TGCGAGCCCG ACACCACTGC GCCATCGGGG 
CAGAACGAGA ATGAGCGCTG TGGCACCGAT CTGGCCTATG TTTACTTTGT CTCCTTCATC 
TTCTTCTGCT CTTTCTTGAT GCTCAACTTG TTTGTGGCCG TCATCATGGA CAACTTCGAG 
TACCTGACTC GGGACTCCTC CATCTTAGGG CCCCACCACT TGGATGAGTT TGTCCGCGTC 
TGGGCAGAGT ACGACCGAGC TGCATGTGGC CGCATCCATT ATACTGAGAT GTATGAAATG 
CTGACTCTCA TGTCACCACC GCTAGGCCTC GGCAAGAGAT GTCCCTCCAA AGTGGCATAT 
AAGAGGTTGG TCCTAATGAA TATGCCAGTA GCTGAGGACA TGACAGTCCA CTTCACCTCC 
ACACTGATGG CTCTGATCCG GACAGCCCTG GACATCAAAA TCGCCAAAGG TGGTGCAGAC 
AGGCAGCAGC TAGACTCGGA GCTGCAAAAG GAGACCCTGG CCATCTGGCC TCACCTGTCC 
CAGAAGATGC TAGACCTGCT TGTGCCCATG CCCAAAGCCT CTGACCTGAC TGTGGGCAAA 
ATCTATGCAG CAATGATGAT CATGGACTAC TATAAACAGA GTAAGGTGAA GAAACAGAGG 
CAGCAGCTGG AGGAACAGAA AAATGCACCC ATGTTCCAGC GAATGGAGCC CTCATCTCTG 
CCTCAGGAGA TCATTGCTAA TGCCAAAGCC CTGCCCTATC TCCAGCAGGA CCCCGTTTCA 
GGCCTGAGTG GCCGTAGTGG ATACCCTTCG ATGAGTCCAC TCTCCCCCCA GGAAATATTC 
CAATTGGCTT GTATGGATCC AGCCGATGAT GGACAGTTCC AGGAACATCA GTCTCTGGAG 
CCAGAGGTTA GTGAATTAAA AAGCGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTTCG 
GACACCCAGG AGCATACGGG ATCTGGGAGG GCATCTTCTA TGCCACGCCT GACTGTGGAT 
CCCCAGGTGG TGACAGATAC TAGCTCCATG AGACGTTCAT TTTCCACTAT TCGGGATAAG 
CGTTCAAATT CCTCCTGGTT GGAGGAATTC TCCATGGAGA GAAGCAGTGA AAACACCTAC 
AAGTCACGTC GTCGGAGTTA CCACTCCTCC CTGAGACTGT CAGCTCATCG CCTGAATTCT 
GACTCAGGCC ACAAGTCTGA CACCCACCGC TCAGGGGGTA GAGAGCGGGG ACGATCGAAA 
GAGCGAAAGC ATCTTCTCTC TCCTGATGTC TCCCGCTGCA ACTCAGAGGA GCGAGGGACC 
CAGGCCGACT GGGAGTCCCC AGAGCGCCAA CAGTCCAGGT CTCCCAGTGA GGCCAGGTCA 
CAGACCCCCA ACAGACAGGG CACGGGCTCC CTGAGCGAGA GCTCCATCCC CTCCATCTCT 
GACACCAGCA CCCCAAGACG AAGTCGCCGG CAGCTCCCAC CCGTCCCACC AAAGCCTCGG 
CCCCTCCTCT CCTACAGCTC CCTGATTCGA CACTCCGGGA GCATCTCCCC GCCGGCCGAC 
GGAAGCGAGA GGGGCTCCCC GCTGACCCCC CAGGCGCTGG AGAGCACGGA GGCCGGCCTG 
GCGGAGCCCT CGGGCCCCCG GCACGGGCAC GGGCAGGGGC ACGGGCAGGG GCACGGGCAG 
CAGAGCCAGC ACTGCTCCCC GCAGCGCTAC ATCTCCGAGC CCTACCTGGC CCTGCACGAA 
GACTCCCACG CGTCGGACTG TGGCGAGGAG GAGACGCTCA CCTTCGAGGC CGCCGTGGCC 
ACCAGCCTGG GCCGCTCCAA CACCATCGGC TCGGCCCCGC CGCTGCGGCA CAGCTGGCAG 
ATGCCCAACG GGCACTATCG GCGGCGGCGG CGCGGGGGGC CGGGGCCGGG CGTGATGTGC 
GGGGCGGTCA GCGACCTCCT GAGTGACACG GAAGAAGACG ACAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_005622393.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622450.1 Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCe09116
Clone ID Related Accession (Same CDS sequence)	XM_005622393.2
Accession Version	XM_005622393.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005622393.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGC AATGTTCTGG GATGGTGCCC GAGATGCGTG GACCGAGCCC AGGCCGCAGG 
CTGCCCGAGC CCGCCCCCTG CCCCGCCGCG GCCGGGAGCC CTGAGCCCTG GGCCGCGGGC 
CGCCGCCGCC ACGTCCTGGC CCTGCCTGAG GAGGAGGCAT GGAGGCCCCA GCAGTGCTCC 
CTGCTCGAGG CCGACGCCTC GGACGAGGTG GGCATGCTGC CGGCCTCCCC GCGCGCCGCC 
GCCTCCGGCG TCGACAACTT CTTCCCGGTG CAGGAGGGCG AGGGCGCGGG CTGGGAGGGC 
GGTGCGGCCC TGGACGCCGG CGCCGGCCCC TTCCTCCCGG TGAGCCCCGA GGTGATGAAG 
CGGCGGCGCG GGGGCCTCAT CGAGCAGCGC GACATCATCA AGGCCCACGA GGCGCACAAG 
ATGCAGAGCA CCCCGCAGGC GCGGCGCAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG 
GTGGTCGGCA GGCCGGGGTC GGGCGATGGA GACTCGGACC AGAGCAGGAA CCGGCAAGGA 
ACCCCGGTGC CGGCCTCGGG GCCGGCGGCC GCCTACAAGC AGTCGAAAGC GCAGAGGGCG 
CGGACTATGG CTTTGTACAA CCCCATTCCC GTCCGGCAGA ACTGTTTCAC CGTCAACAGA 
TCCCTGTTCA TCTTCGGAGA AGATAACATT GTCAGGAAAT ACGCCAAGAA GCTCATCGAC 
TGGCCGCCGT TTGAGTACAT GATTTTGGCA ACCATCATTG CCAACTGCAT TGTCTTGGCC 
CTGGAACAGC ATCTTCCTGA GGATGACAAG ACCCCGATGT CCCGCAGACT GGAGAAGACA 
GAGCCGTATT TCATTGGGAT CTTTTGCTTT GAAGCCGGTA TCAAAATTGT GGCCCTGGGG 
TTCATCTTTC ATAAGGGCTC GTACCTCCGC AATGGCTGGA ATGTCATGGA CTTCATCGTG 
GTCCTCAGTG GCATCCTGGC CACTGCGGGG ACTCACTTCA ACACACACGT GGACCTGAGG 
ACCCTCCGGG CTGTGCGTGT CCTGAGACCC CTGAAGCTCG TGTCAGGGAT TCCTAGCCTG 
CAGATCGTGT TGAAGTCTAT CATGAAGGCC ATGGTGCCTC TTCTGCAAAT TGGTCTCCTG 
CTCTTCTTTG CCATCTTGAT GTTTGCTATC ATTGGCTTAG AGTTCTACAG TGGGAAGTTG 
CACCGAGCAT GTTTCACGAA CAATTCAGGT ATTCTGGAAG GATTTGACCC CCCTCACCCA 
TGTGGTGTGC AGGGCTGCCC AGCTGGCTAC GAATGCCGGG ACTGGATTGG CCCCAATGAT 
GGGATCACCC AGTTTGATAA CATCCTTTTT GCTGTGCTGA CTGTCTTCCA ATGCATCACC 
ATGGAAGGAT GGACCACCGT GCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC 
TGGCTGTACT TCATCCCCCT CATCATCATT GGATCCTTCT TTGTTCTCAA CCTAGTTCTG 
GGAGTGCTTT CCGGGGAGTT TGCCAAAGAG AGAGAGAGAG TGGAGAACCG GAGAGCTTTC 
ATGAAACTGC GGCGGCAGCA GCAGATTGAG CGGGAGCTGA ACGGGTACCG CGCCTGGATA 
GACAAAGCAG AGGAAGTCAT GCTTGCTGAA GAAAATAAAA ATGCTGGGAC GTCAGCCTTA 
GAAGTGCTTC GAAGGGCAAC CATCAAAAGG AGCCGGACAG AGGCCATGAC TCGAGACTCC 
AGTGATGAAC ACTGTGTCGA TATCTCCTCT GTGGGCACAC CTCTCGCCCG AGCCAGCATC 
AAAAGTGCAA AGGTGGATGG GGCCTCCTAT TTCCGGCACA AAGAAAGGCT TCTGCGCATC 
TCTATCCGCC ACATGGTGAA ATCCCAGGTG TTTTACTGGA TTGTGTTGAG CCTTGTGGCT 
CTCAACACTG CCTGTGTGGC CATCGTCCAT CATAACCAGC CCCAGTGGCT CACCCATCTC 
CTCTACTATG CAGAATTTTT GTTTCTGGGA CTGTTCCTCT TGGAGATGTC CCTGAAGATG 
TATGGCATGG GACCTCGACT TTATTTTCAC TCTTCATTCA ACTGTTTTGA TTTTGGGGTC 
ACAGTGGGCA GCATCTTTGA AGTGGTCTGG GCAATCTTCA GACCTGGCAC ATCTTTTGGA 
ATCAGTGTCT TACGGGCTCT CCGGCTTCTA AGAATATTTA AAATAACCAA GTATTGGGCA 
TCTCTGCGAA ATTTGGTGGT CTCCTTGATG AGCTCTATGA AGTCTATCAT CAGTTTGCTC 
TTCCTCCTCT TCCTCTTCAT CGTTGTCTTT GCTCTCCTAG GAATGCAGCT GTTTGGAGGC 
AGGTTTAACT TTAATGATGG GACTCCTTCA GCAAACTTTG ATACCTTCCC TGCAGCCATC 
ATGACTGTAT TCCAGATCCT AACAGGTGAG GACTGGAATG AGGTGATGTA CAATGGGATC 
CGTTCCCAGG GTGGAGTTAG CTCAGGCATG TGGTCAGCTA TCTATTTCAT TGTGCTCACC 
TTGTTTGGAA ACTACACACT GCTGAATGTG TTCTTGGCCA TTGCTGTGGA CAATCTGGCC 
AATGCCCAAG AGCTGACCAA GGATGAACAA GAAGAAGAGG AGGCTTTTAA CCAGAAACAT 
GCATTGCAGA AGGCCAAGGA GGTCAGCCCG ATGTCTGCAC CCAACATGCC TTCTATCGAG 
AGGGAGCGGA GGCGCCGGCA CCACATGTCG GTGTGGGAGC AGCGCACCAG CCAGCTGAGA 
AGGCACATGC AGATGTCCAG CCAGGAGGCC CTCAATAAAG AGGAGGCCCC ACCGATGAAC 
CCCCTCAACC CCCTCAACCC GCTGAGCCCC CTCAACCCGC TCAACGCCCA CCCCAGCCTC 
TACCGGAGAC CTAGGCCTAT TGAGGGCCTG GGCCTGGGCC TGGAGAAGTG TGAGGAGGAG 
CGCATCATCA GCCGCGGGGG GTCCCTCAAG GGGGACGGAG GGGACCTGTC GAGTGCCCTG 
GACAACCAGA GGAGCCCTTT GTCCCTGGGC AAACGGGAGC CACCATGGCT GGCCAGGCCC 
TGTCATGGGA ACTGTGATCC GACCCAGCAG GAGGCTGGGG GAGGGGAGAC CGTGGTGACC 
TTCGAGGACC GGGCCAGGCA CAGGCAGAGC CAGCGGCGCA GCCGGCACCG TCGTGTCAGG 
ACGGAAGGCA AGGAGCTCTC CTCCACCTCC CGGAGCAGGT CTGTCAGCCA GGAGCGGAGT 
CTGGATGAGG GGGTGCTCAC CGACGGGGAG AAGGAGCGAG AGCCCAGGGG CGGCCATGCA 
GGCAAAGAAC CCACCATCCA AGAAGAAGAC AGAGGCCAAG ACTTAAGGAG GACGAACAGT 
CTGATGGTGC CCAGAGGCTC TGGGCTGGCA GGAGCCCTTG ATGAGGCCAA CACACCCCTA 
GTCCTGCCCC ACCACGATCT GGAAGTGGGG AAGGACACGG TGCTGACGGA GCAGGAGCCG 
GAGGGCAGCA GCGAGCAGGC CCTGCTGGGG GATGTGCAGT TGGACATTGG CAGGGTCATC 
AGCCAGAGTG AGCCTGACCT CTCCTGCGTC ACAGCCAACA CAGACAAGGC CACCACCGAC 
AGCACCAGCG TCACCGTCGC CATCCCTGAT GTGGGCCCCT TGATGGACTC AACTGTGATT 
CACATTAGCA ACAAAACTGA TGGAGAAGCC AGTCCCTTGA AGGAGGCTGA GATCAAAGAG 
GAGGAGGAGG AAATGGAGAA GAAGCAGCAG AAGAAGGAGA AGCGTGAGAC GGGCAAAGCC 
ATGGTGCCCC ACAGCTCTAT GTTCATCTTC AGCACCACCA ACCCGATTCG CAGGGCCTGC 
CACTACATCG TGAACCTGCG CTACTTTGAG ATGTGCATTC TCCTGGTGAT CGCCGCCAGC 
AGCATTGCCC TGGCAGCAGA GGACCCCGTC CTGACCAACT CGGAGCGCAA CAAAGTGGGT 
TCACGGGAGC CGTTTGCCAA CCTCCGTAAT GCCCTGGTCC TGAGGTATTT TGACTATGTT 
TTCACTGGCG TGTTCACCTT TGAGATGGTT ATAAAGATGA TAGACCAGGG CTTGATCCTA 
CAGGATGGGT CCTACTTCCG AGACCTGTGG AACATCCTGG ACTTTGTGGT GGTGGTTGGT 
GCATTGGTGG CCTTTGCTTT GGCGAACGCT TTGGGAACAA ACAAGGGGCG AGACATCAAG 
ACCATCAAAT CCCTGAGGGT GCTCCGAGTT CTGAGGCCAC TGAAGACCAT CAAGCGCTTG 
CCCAAACTCA AGGCTGTCTT TGACTGTGTG GTGACCTCCT TGAAGAATGT CTTCAACATA 
CTCATCGTCT ATAAGCTCTT CATGTTCATC TTTGCTGTCA TTGCGGTTCA GCTCTTCAAG 
GGAAAATTCT TTTATTGCAC AGACAGTTCC AAGGACACAG AGAAGGAGTG CATAGGCAAC 
TACGTAGATC ATGAAAAAAA CAAGATGGAG GTGAAAGGCC GGGAATGGAA GCGCCATGAA 
TTCCACTACG ACAACATTAT CTGGGCCCTG CTGACCCTCT TCACCGTCTC CACGGGGGAA 
GGATGGCCTC AAGTCTTACA GCATTCTGTC GATGTGACAG AGGAAGACCG AGGACCAAGC 
CGCAGCAACC GCATGGAGAT GTCCATCTTC TATGTGGTCT ACTTTGTGGT CTTCCCTTTC 
TTCTTTGTCA ATATCTTTGT GGCTCTGATC ATCATCACCT TCCAGGAACA AGGGGACAAG 
ATGATGGAGG AATGCAGCCT GGAGAAGAAC GAGAGGGCGT GCATTGACTT CGCCATCAGT 
GCCAAACCTC TCACCCGCTA CATGCCACAG AACAGGCACA CCTTCCAGTA CCGCGTCTGG 
CACTTCGTGG TGTCTCCGTC CTTTGAGTAC ACCATCATGG CCATGATCGC CCTGAACACC 
GTCGTGCTGA TGATGAAGTA CTACTCTGCT CCCTGTACCT ATGAACTGGC CCTGAAGTAC 
CTGAATATTG CCTTCACCAT GGTGTTTTCC CTGGAATGTG TCCTAAAGAT CATCGCTTTT 
GGCTTCTTGA ACTACTTTCG AGACACCTGG AACATCTTTG ACTTCATCAC TGTGATTGGC 
AGTATCACAG AAATCATACT GACAGACAGC AAGCTGGTAA ACACCAGTGG TTTCAACATG 
AGCTTTCTGA AGCTCTTCCG TGCCGCCCGC CTCATCAAGC TTCTGCGGCA GGGCTACACC 
ATCCGTATTT TACTCTGGAC CTTCGTGCAG TCCTTTAAGG CCCTCCCCTA CGTCTGCCTT 
TTGATTGCCA TGCTTTTCTT CATCTATGCC ATCATTGGGA TGCAGGTATT TGGAAACATA 
AAATTAGATG AGGAGAGTCA CATCAACCGG CACAACAACT TCCGGAGTTT CTTTGGGTCC 
CTCATGTTAC TCTTCAGGAG TGCCACGGGA GAGGCCTGGC AGGAGATAAT GCTGTCGTGC 
CTCGGGGAGA AAGGCTGCGA GCCCGACACC ACTGCGCCAT CGGGGCAGAA CGAGAATGAG 
CGCTGTGGCA CCGATCTGGC CTATGTTTAC TTTGTCTCCT TCATCTTCTT CTGCTCTTTC 
TTGATGCTCA ACTTGTTTGT GGCCGTCATC ATGGACAACT TCGAGTACCT GACTCGGGAC 
TCCTCCATCT TAGGGCCCCA CCACTTGGAT GAGTTTGTCC GCGTCTGGGC AGAGTACGAC 
CGAGCTGCAT GTGGCCGCAT CCATTATACT GAGATGTATG AAATGCTGAC TCTCATGTCA 
CCACCGCTAG GCCTCGGCAA GAGATGTCCC TCCAAAGTGG CATATAAGAG GTTGGTCCTA 
ATGAATATGC CAGTAGCTGA GGACATGACA GTCCACTTCA CCTCCACACT GATGGCTCTG 
ATCCGGACAG CCCTGGACAT CAAAATCGCC AAAGGTGGTG CAGACAGGCA GCAGCTAGAC 
TCGGAGCTGC AAAAGGAGAC CCTGGCCATC TGGCCTCACC TGTCCCAGAA GATGCTAGAC 
CTGCTTGTGC CCATGCCCAA AGCCTCTGAC CTGACTGTGG GCAAAATCTA TGCAGCAATG 
ATGATCATGG ACTACTATAA ACAGAGTAAG GTGAAGAAAC AGAGGCAGCA GCTGGAGGAA 
CAGAAAAATG CACCCATGTT CCAGCGAATG GAGCCCTCAT CTCTGCCTCA GGAGATCATT 
GCTAATGCCA AAGCCCTGCC CTATCTCCAG CAGGACCCCG TTTCAGGCCT GAGTGGCCGT 
AGTGGATACC CTTCGATGAG TCCACTCTCC CCCCAGGAAA TATTCCAATT GGCTTGTATG 
GATCCAGCCG ATGATGGACA GTTCCAGGAA CATCAGTCTC TGGAGCCAGA GGTTAGTGAA 
TTAAAAAGCG TGCAGTCCTC TAACCATGGC ATCTACCTTC CTTCGGACAC CCAGGAGCAT 
ACGGGATCTG GGAGGGCATC TTCTATGCCA CGCCTGACTG TGGATCCCCA GGTGGTGACA 
GATACTAGCT CCATGAGACG TTCATTTTCC ACTATTCGGG ATAAGCGTTC AAATTCCTCC 
TGGTTGGAGG AATTCTCCAT GGAGAGAAGC AGTGAAAACA CCTACAAGTC ACGTCGTCGG 
AGTTACCACT CCTCCCTGAG ACTGTCAGCT CATCGCCTGA ATTCTGACTC AGGCCACAAG 
TCTGACACCC ACCGCTCAGG GGGTAGAGAG CGGGGACGAT CGAAAGAGCG AAAGCATCTT 
CTCTCTCCTG ATGTCTCCCG CTGCAACTCA GAGGAGCGAG GGACCCAGGC CGACTGGGAG 
TCCCCAGAGC GCCAACAGTC CAGGTCTCCC AGTGAGGCCA GGTCACAGAC CCCCAACAGA 
CAGGGCACGG GCTCCCTGAG CGAGAGCTCC ATCCCCTCCA TCTCTGACAC CAGCACCCCA 
AGACGAAGTC GCCGGCAGCT CCCACCCGTC CCACCAAAGC CTCGGCCCCT CCTCTCCTAC 
AGCTCCCTGA TTCGACACTC CGGGAGCATC TCCCCGCCGG CCGACGGAAG CGAGAGGGGC 
TCCCCGCTGA CCCCCCAGGC GCTGGAGAGC ACGGAGGCCG GCCTGGCGGA GCCCTCGGGC 
CCCCGGCACG GGCACGGGCA GGGGCACGGG CAGGGGCACG GGCAGCAGAG CCAGCACTGC 
TCCCCGCAGC GCTACATCTC CGAGCCCTAC CTGGCCCTGC ACGAAGACTC CCACGCGTCG 
GACTGTGGCG AGGAGGAGAC GCTCACCTTC GAGGCCGCCG TGGCCACCAG CCTGGGCCGC 
TCCAACACCA TCGGCTCGGC CCCGCCGCTG CGGCACAGCT GGCAGATGCC CAACGGGCAC 
TATCGGCGGC GGCGGCGCGG GGGGCCGGGG CCGGGCGTGA TGTGCGGGGC GGTCAGCGAC 
CTCCTGAGTG ACACGGAAGA AGACGACAAG TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622450.1
CDS	86..7501
Translation	MEEQCSGMVPEMRGPSPGRRLPEPAPCPAAAGSPEPWAAGRRRHVLALPEEEAWRPQQCSLLEADASDEVGMLPASPRAAASGVDNFFPVQEGEGAGWEGGAALDAGAGPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFTNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLGLGLEKCEEERIISRGGSLKGDGGDLSSALDNQRSPLSLGKREPPWLARPCHGNCDPTQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKELSSTSRSRSVSQERSLDEGVLTDGEKEREPRGGHAGKEPTIQEEDRGQDLRRTNSLMVPRGSGLAGALDEANTPLVLPHHDLEVGKDTVLTEQEPEGSSEQALLGDVQLDIGRVISQSEPDLSCVTANTDKATTDSTSVTVAIPDVGPLMDSTVIHISNKTDGEASPLKEAEIKEEEEEMEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVGSREPFANLRNALVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEHQSLEPEVSELKSVQSSNHGIYLPSDTQEHTGSGRASSMPRLTVDPQVVTDTSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERQQSRSPSEARSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHSGSISPPADGSERGSPLTPQALESTEAGLAEPSGPRHGHGQGHGQGHGQQSQHCSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGVMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_005622393.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622393.2

ORF Insert Sequence:

ATGGAAGAGC AATGTTCTGG GATGGTGCCC GAGATGCGTG GACCGAGCCC AGGCCGCAGG 
CTGCCCGAGC CCGCCCCCTG CCCCGCCGCG GCCGGGAGCC CTGAGCCCTG GGCCGCGGGC 
CGCCGCCGCC ACGTCCTGGC CCTGCCTGAG GAGGAGGCAT GGAGGCCCCA GCAGTGCTCC 
CTGCTCGAGG CCGACGCCTC GGACGAGGTG GGCATGCTGC CGGCCTCCCC GCGCGCCGCC 
GCCTCCGGCG TCGACAACTT CTTCCCGGTG CAGGAGGGCG AGGGCGCGGG CTGGGAGGGC 
GGTGCGGCCC TGGACGCCGG CGCCGGCCCC TTCCTCCCGG TGAGCCCCGA GGTGATGAAG 
CGGCGGCGCG GGGGCCTCAT CGAGCAGCGC GACATCATCA AGGCCCACGA GGCGCACAAG 
ATGCAGAGCA CCCCGCAGGC GCGGCGCAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG 
GTGGTCGGCA GGCCGGGGTC GGGCGATGGA GACTCGGACC AGAGCAGGAA CCGGCAAGGA 
ACCCCGGTGC CGGCCTCGGG GCCGGCGGCC GCCTACAAGC AGTCGAAAGC GCAGAGGGCG 
CGGACTATGG CTTTGTACAA CCCCATTCCC GTCCGGCAGA ACTGTTTCAC CGTCAACAGA 
TCCCTGTTCA TCTTCGGAGA AGATAACATT GTCAGGAAAT ACGCCAAGAA GCTCATCGAC 
TGGCCGCCGT TTGAGTACAT GATTTTGGCA ACCATCATTG CCAACTGCAT TGTCTTGGCC 
CTGGAACAGC ATCTTCCTGA GGATGACAAG ACCCCGATGT CCCGCAGACT GGAGAAGACA 
GAGCCGTATT TCATTGGGAT CTTTTGCTTT GAAGCCGGTA TCAAAATTGT GGCCCTGGGG 
TTCATCTTTC ATAAGGGCTC GTACCTCCGC AATGGCTGGA ATGTCATGGA CTTCATCGTG 
GTCCTCAGTG GCATCCTGGC CACTGCGGGG ACTCACTTCA ACACACACGT GGACCTGAGG 
ACCCTCCGGG CTGTGCGTGT CCTGAGACCC CTGAAGCTCG TGTCAGGGAT TCCTAGCCTG 
CAGATCGTGT TGAAGTCTAT CATGAAGGCC ATGGTGCCTC TTCTGCAAAT TGGTCTCCTG 
CTCTTCTTTG CCATCTTGAT GTTTGCTATC ATTGGCTTAG AGTTCTACAG TGGGAAGTTG 
CACCGAGCAT GTTTCACGAA CAATTCAGGT ATTCTGGAAG GATTTGACCC CCCTCACCCA 
TGTGGTGTGC AGGGCTGCCC AGCTGGCTAC GAATGCCGGG ACTGGATTGG CCCCAATGAT 
GGGATCACCC AGTTTGATAA CATCCTTTTT GCTGTGCTGA CTGTCTTCCA ATGCATCACC 
ATGGAAGGAT GGACCACCGT GCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC 
TGGCTGTACT TCATCCCCCT CATCATCATT GGATCCTTCT TTGTTCTCAA CCTAGTTCTG 
GGAGTGCTTT CCGGGGAGTT TGCCAAAGAG AGAGAGAGAG TGGAGAACCG GAGAGCTTTC 
ATGAAACTGC GGCGGCAGCA GCAGATTGAG CGGGAGCTGA ACGGGTACCG CGCCTGGATA 
GACAAAGCAG AGGAAGTCAT GCTTGCTGAA GAAAATAAAA ATGCTGGGAC GTCAGCCTTA 
GAAGTGCTTC GAAGGGCAAC CATCAAAAGG AGCCGGACAG AGGCCATGAC TCGAGACTCC 
AGTGATGAAC ACTGTGTCGA TATCTCCTCT GTGGGCACAC CTCTCGCCCG AGCCAGCATC 
AAAAGTGCAA AGGTGGATGG GGCCTCCTAT TTCCGGCACA AAGAAAGGCT TCTGCGCATC 
TCTATCCGCC ACATGGTGAA ATCCCAGGTG TTTTACTGGA TTGTGTTGAG CCTTGTGGCT 
CTCAACACTG CCTGTGTGGC CATCGTCCAT CATAACCAGC CCCAGTGGCT CACCCATCTC 
CTCTACTATG CAGAATTTTT GTTTCTGGGA CTGTTCCTCT TGGAGATGTC CCTGAAGATG 
TATGGCATGG GACCTCGACT TTATTTTCAC TCTTCATTCA ACTGTTTTGA TTTTGGGGTC 
ACAGTGGGCA GCATCTTTGA AGTGGTCTGG GCAATCTTCA GACCTGGCAC ATCTTTTGGA 
ATCAGTGTCT TACGGGCTCT CCGGCTTCTA AGAATATTTA AAATAACCAA GTATTGGGCA 
TCTCTGCGAA ATTTGGTGGT CTCCTTGATG AGCTCTATGA AGTCTATCAT CAGTTTGCTC 
TTCCTCCTCT TCCTCTTCAT CGTTGTCTTT GCTCTCCTAG GAATGCAGCT GTTTGGAGGC 
AGGTTTAACT TTAATGATGG GACTCCTTCA GCAAACTTTG ATACCTTCCC TGCAGCCATC 
ATGACTGTAT TCCAGATCCT AACAGGTGAG GACTGGAATG AGGTGATGTA CAATGGGATC 
CGTTCCCAGG GTGGAGTTAG CTCAGGCATG TGGTCAGCTA TCTATTTCAT TGTGCTCACC 
TTGTTTGGAA ACTACACACT GCTGAATGTG TTCTTGGCCA TTGCTGTGGA CAATCTGGCC 
AATGCCCAAG AGCTGACCAA GGATGAACAA GAAGAAGAGG AGGCTTTTAA CCAGAAACAT 
GCATTGCAGA AGGCCAAGGA GGTCAGCCCG ATGTCTGCAC CCAACATGCC TTCTATCGAG 
AGGGAGCGGA GGCGCCGGCA CCACATGTCG GTGTGGGAGC AGCGCACCAG CCAGCTGAGA 
AGGCACATGC AGATGTCCAG CCAGGAGGCC CTCAATAAAG AGGAGGCCCC ACCGATGAAC 
CCCCTCAACC CCCTCAACCC GCTGAGCCCC CTCAACCCGC TCAACGCCCA CCCCAGCCTC 
TACCGGAGAC CTAGGCCTAT TGAGGGCCTG GGCCTGGGCC TGGAGAAGTG TGAGGAGGAG 
CGCATCATCA GCCGCGGGGG GTCCCTCAAG GGGGACGGAG GGGACCTGTC GAGTGCCCTG 
GACAACCAGA GGAGCCCTTT GTCCCTGGGC AAACGGGAGC CACCATGGCT GGCCAGGCCC 
TGTCATGGGA ACTGTGATCC GACCCAGCAG GAGGCTGGGG GAGGGGAGAC CGTGGTGACC 
TTCGAGGACC GGGCCAGGCA CAGGCAGAGC CAGCGGCGCA GCCGGCACCG TCGTGTCAGG 
ACGGAAGGCA AGGAGCTCTC CTCCACCTCC CGGAGCAGGT CTGTCAGCCA GGAGCGGAGT 
CTGGATGAGG GGGTGCTCAC CGACGGGGAG AAGGAGCGAG AGCCCAGGGG CGGCCATGCA 
GGCAAAGAAC CCACCATCCA AGAAGAAGAC AGAGGCCAAG ACTTAAGGAG GACGAACAGT 
CTGATGGTGC CCAGAGGCTC TGGGCTGGCA GGAGCCCTTG ATGAGGCCAA CACACCCCTA 
GTCCTGCCCC ACCACGATCT GGAAGTGGGG AAGGACACGG TGCTGACGGA GCAGGAGCCG 
GAGGGCAGCA GCGAGCAGGC CCTGCTGGGG GATGTGCAGT TGGACATTGG CAGGGTCATC 
AGCCAGAGTG AGCCTGACCT CTCCTGCGTC ACAGCCAACA CAGACAAGGC CACCACCGAC 
AGCACCAGCG TCACCGTCGC CATCCCTGAT GTGGGCCCCT TGATGGACTC AACTGTGATT 
CACATTAGCA ACAAAACTGA TGGAGAAGCC AGTCCCTTGA AGGAGGCTGA GATCAAAGAG 
GAGGAGGAGG AAATGGAGAA GAAGCAGCAG AAGAAGGAGA AGCGTGAGAC GGGCAAAGCC 
ATGGTGCCCC ACAGCTCTAT GTTCATCTTC AGCACCACCA ACCCGATTCG CAGGGCCTGC 
CACTACATCG TGAACCTGCG CTACTTTGAG ATGTGCATTC TCCTGGTGAT CGCCGCCAGC 
AGCATTGCCC TGGCAGCAGA GGACCCCGTC CTGACCAACT CGGAGCGCAA CAAAGTGGGT 
TCACGGGAGC CGTTTGCCAA CCTCCGTAAT GCCCTGGTCC TGAGGTATTT TGACTATGTT 
TTCACTGGCG TGTTCACCTT TGAGATGGTT ATAAAGATGA TAGACCAGGG CTTGATCCTA 
CAGGATGGGT CCTACTTCCG AGACCTGTGG AACATCCTGG ACTTTGTGGT GGTGGTTGGT 
GCATTGGTGG CCTTTGCTTT GGCGAACGCT TTGGGAACAA ACAAGGGGCG AGACATCAAG 
ACCATCAAAT CCCTGAGGGT GCTCCGAGTT CTGAGGCCAC TGAAGACCAT CAAGCGCTTG 
CCCAAACTCA AGGCTGTCTT TGACTGTGTG GTGACCTCCT TGAAGAATGT CTTCAACATA 
CTCATCGTCT ATAAGCTCTT CATGTTCATC TTTGCTGTCA TTGCGGTTCA GCTCTTCAAG 
GGAAAATTCT TTTATTGCAC AGACAGTTCC AAGGACACAG AGAAGGAGTG CATAGGCAAC 
TACGTAGATC ATGAAAAAAA CAAGATGGAG GTGAAAGGCC GGGAATGGAA GCGCCATGAA 
TTCCACTACG ACAACATTAT CTGGGCCCTG CTGACCCTCT TCACCGTCTC CACGGGGGAA 
GGATGGCCTC AAGTCTTACA GCATTCTGTC GATGTGACAG AGGAAGACCG AGGACCAAGC 
CGCAGCAACC GCATGGAGAT GTCCATCTTC TATGTGGTCT ACTTTGTGGT CTTCCCTTTC 
TTCTTTGTCA ATATCTTTGT GGCTCTGATC ATCATCACCT TCCAGGAACA AGGGGACAAG 
ATGATGGAGG AATGCAGCCT GGAGAAGAAC GAGAGGGCGT GCATTGACTT CGCCATCAGT 
GCCAAACCTC TCACCCGCTA CATGCCACAG AACAGGCACA CCTTCCAGTA CCGCGTCTGG 
CACTTCGTGG TGTCTCCGTC CTTTGAGTAC ACCATCATGG CCATGATCGC CCTGAACACC 
GTCGTGCTGA TGATGAAGTA CTACTCTGCT CCCTGTACCT ATGAACTGGC CCTGAAGTAC 
CTGAATATTG CCTTCACCAT GGTGTTTTCC CTGGAATGTG TCCTAAAGAT CATCGCTTTT 
GGCTTCTTGA ACTACTTTCG AGACACCTGG AACATCTTTG ACTTCATCAC TGTGATTGGC 
AGTATCACAG AAATCATACT GACAGACAGC AAGCTGGTAA ACACCAGTGG TTTCAACATG 
AGCTTTCTGA AGCTCTTCCG TGCCGCCCGC CTCATCAAGC TTCTGCGGCA GGGCTACACC 
ATCCGTATTT TACTCTGGAC CTTCGTGCAG TCCTTTAAGG CCCTCCCCTA CGTCTGCCTT 
TTGATTGCCA TGCTTTTCTT CATCTATGCC ATCATTGGGA TGCAGGTATT TGGAAACATA 
AAATTAGATG AGGAGAGTCA CATCAACCGG CACAACAACT TCCGGAGTTT CTTTGGGTCC 
CTCATGTTAC TCTTCAGGAG TGCCACGGGA GAGGCCTGGC AGGAGATAAT GCTGTCGTGC 
CTCGGGGAGA AAGGCTGCGA GCCCGACACC ACTGCGCCAT CGGGGCAGAA CGAGAATGAG 
CGCTGTGGCA CCGATCTGGC CTATGTTTAC TTTGTCTCCT TCATCTTCTT CTGCTCTTTC 
TTGATGCTCA ACTTGTTTGT GGCCGTCATC ATGGACAACT TCGAGTACCT GACTCGGGAC 
TCCTCCATCT TAGGGCCCCA CCACTTGGAT GAGTTTGTCC GCGTCTGGGC AGAGTACGAC 
CGAGCTGCAT GTGGCCGCAT CCATTATACT GAGATGTATG AAATGCTGAC TCTCATGTCA 
CCACCGCTAG GCCTCGGCAA GAGATGTCCC TCCAAAGTGG CATATAAGAG GTTGGTCCTA 
ATGAATATGC CAGTAGCTGA GGACATGACA GTCCACTTCA CCTCCACACT GATGGCTCTG 
ATCCGGACAG CCCTGGACAT CAAAATCGCC AAAGGTGGTG CAGACAGGCA GCAGCTAGAC 
TCGGAGCTGC AAAAGGAGAC CCTGGCCATC TGGCCTCACC TGTCCCAGAA GATGCTAGAC 
CTGCTTGTGC CCATGCCCAA AGCCTCTGAC CTGACTGTGG GCAAAATCTA TGCAGCAATG 
ATGATCATGG ACTACTATAA ACAGAGTAAG GTGAAGAAAC AGAGGCAGCA GCTGGAGGAA 
CAGAAAAATG CACCCATGTT CCAGCGAATG GAGCCCTCAT CTCTGCCTCA GGAGATCATT 
GCTAATGCCA AAGCCCTGCC CTATCTCCAG CAGGACCCCG TTTCAGGCCT GAGTGGCCGT 
AGTGGATACC CTTCGATGAG TCCACTCTCC CCCCAGGAAA TATTCCAATT GGCTTGTATG 
GATCCAGCCG ATGATGGACA GTTCCAGGAA CATCAGTCTC TGGAGCCAGA GGTTAGTGAA 
TTAAAAAGCG TGCAGTCCTC TAACCATGGC ATCTACCTTC CTTCGGACAC CCAGGAGCAT 
ACGGGATCTG GGAGGGCATC TTCTATGCCA CGCCTGACTG TGGATCCCCA GGTGGTGACA 
GATACTAGCT CCATGAGACG TTCATTTTCC ACTATTCGGG ATAAGCGTTC AAATTCCTCC 
TGGTTGGAGG AATTCTCCAT GGAGAGAAGC AGTGAAAACA CCTACAAGTC ACGTCGTCGG 
AGTTACCACT CCTCCCTGAG ACTGTCAGCT CATCGCCTGA ATTCTGACTC AGGCCACAAG 
TCTGACACCC ACCGCTCAGG GGGTAGAGAG CGGGGACGAT CGAAAGAGCG AAAGCATCTT 
CTCTCTCCTG ATGTCTCCCG CTGCAACTCA GAGGAGCGAG GGACCCAGGC CGACTGGGAG 
TCCCCAGAGC GCCAACAGTC CAGGTCTCCC AGTGAGGCCA GGTCACAGAC CCCCAACAGA 
CAGGGCACGG GCTCCCTGAG CGAGAGCTCC ATCCCCTCCA TCTCTGACAC CAGCACCCCA 
AGACGAAGTC GCCGGCAGCT CCCACCCGTC CCACCAAAGC CTCGGCCCCT CCTCTCCTAC 
AGCTCCCTGA TTCGACACTC CGGGAGCATC TCCCCGCCGG CCGACGGAAG CGAGAGGGGC 
TCCCCGCTGA CCCCCCAGGC GCTGGAGAGC ACGGAGGCCG GCCTGGCGGA GCCCTCGGGC 
CCCCGGCACG GGCACGGGCA GGGGCACGGG CAGGGGCACG GGCAGCAGAG CCAGCACTGC 
TCCCCGCAGC GCTACATCTC CGAGCCCTAC CTGGCCCTGC ACGAAGACTC CCACGCGTCG 
GACTGTGGCG AGGAGGAGAC GCTCACCTTC GAGGCCGCCG TGGCCACCAG CCTGGGCCGC 
TCCAACACCA TCGGCTCGGC CCCGCCGCTG CGGCACAGCT GGCAGATGCC CAACGGGCAC 
TATCGGCGGC GGCGGCGCGG GGGGCCGGGG CCGGGCGTGA TGTGCGGGGC GGTCAGCGAC 
CTCCTGAGTG ACACGGAAGA AGACGACAAG TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_005622394.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622451.1 Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCe09117
Clone ID Related Accession (Same CDS sequence)	XM_005622394.2
Accession Version	XM_005622394.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005622394.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGC AATGTTCTGG GATGGTGCCC GAGATGCGTG GACCGAGCCC AGGCCGCAGG 
CTGCCCGAGC CCGCCCCCTG CCCCGCCGCG GCCGGGAGCC CTGAGCCCTG GGCCGCGGGC 
CGCCGCCGCC ACGTCCTGGC CCTGCCTGAG GAGGAGGCAT GGAGGCCCCA GCAGTGCTCC 
CTGCTCGAGG CCGACGCCTC GGACGAGGTG GGCATGCTGC CGGCCTCCCC GCGCGCCGCC 
GCCTCCGGCG TCGACAACTT CTTCCCGGTG CAGGAGGGCG AGGGCGCGGG CTGGGAGGGC 
GGTGCGGCCC TGGACGCCGG CGCCGGCCCC TTCCTCCCGG TGAGCCCCGA GGTGATGAAG 
CGGCGGCGCG GGGGCCTCAT CGAGCAGCGC GACATCATCA AGGCCCACGA GGCGCACAAG 
ATGCAGAGCA CCCCGCAGGC GCGGCGCAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG 
GTGGTCGGCA GGCCGGGGTC GGGCGATGGA GACTCGGACC AGAGCAGGAA CCGGCAAGGA 
ACCCCGGTGC CGGCCTCGGG GCCGGCGGCC GCCTACAAGC AGTCGAAAGC GCAGAGGGCG 
CGGACTATGG CTTTGTACAA CCCCATTCCC GTCCGGCAGA ACTGTTTCAC CGTCAACAGA 
TCCCTGTTCA TCTTCGGAGA AGATAACATT GTCAGGAAAT ACGCCAAGAA GCTCATCGAC 
TGGCCGCCGT TTGAGTACAT GATTTTGGCA ACCATCATTG CCAACTGCAT TGTCTTGGCC 
CTGGAACAGC ATCTTCCTGA GGATGACAAG ACCCCGATGT CCCGCAGACT GGAGAAGACA 
GAGCCGTATT TCATTGGGAT CTTTTGCTTT GAAGCCGGTA TCAAAATTGT GGCCCTGGGG 
TTCATCTTTC ATAAGGGCTC GTACCTCCGC AATGGCTGGA ATGTCATGGA CTTCATCGTG 
GTCCTCAGTG GCATCCTGGC CACTGCGGGG ACTCACTTCA ACACACACGT GGACCTGAGG 
ACCCTCCGGG CTGTGCGTGT CCTGAGACCC CTGAAGCTCG TGTCAGGGAT TCCTAGCCTG 
CAGATCGTGT TGAAGTCTAT CATGAAGGCC ATGGTGCCTC TTCTGCAAAT TGGTCTCCTG 
CTCTTCTTTG CCATCTTGAT GTTTGCTATC ATTGGCTTAG AGTTCTACAG TGGGAAGTTG 
CACCGAGCAT GTTTCACGAA CAATTCAGGT ATTCTGGAAG GATTTGACCC CCCTCACCCA 
TGTGGTGTGC AGGGCTGCCC AGCTGGCTAC GAATGCCGGG ACTGGATTGG CCCCAATGAT 
GGGATCACCC AGTTTGATAA CATCCTTTTT GCTGTGCTGA CTGTCTTCCA ATGCATCACC 
ATGGAAGGAT GGACCACCGT GCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC 
TGGCTGTACT TCATCCCCCT CATCATCATT GGATCCTTCT TTGTTCTCAA CCTAGTTCTG 
GGAGTGCTTT CCGGGGAGTT TGCCAAAGAG AGAGAGAGAG TGGAGAACCG GAGAGCTTTC 
ATGAAACTGC GGCGGCAGCA GCAGATTGAG CGGGAGCTGA ACGGGTACCG CGCCTGGATA 
GACAAAGCAG AGGAAGTCAT GCTTGCTGAA GAAAATAAAA ATGCTGGGAC GTCAGCCTTA 
GAAGTGCTTC GAAGGGCAAC CATCAAAAGG AGCCGGACAG AGGCCATGAC TCGAGACTCC 
AGTGATGAAC ACTGTGTCGA TATCTCCTCT GTGGGCACAC CTCTCGCCCG AGCCAGCATC 
AAAAGTGCAA AGGTGGATGG GGCCTCCTAT TTCCGGCACA AAGAAAGGCT TCTGCGCATC 
TCTATCCGCC ACATGGTGAA ATCCCAGGTG TTTTACTGGA TTGTGTTGAG CCTTGTGGCT 
CTCAACACTG CCTGTGTGGC CATCGTCCAT CATAACCAGC CCCAGTGGCT CACCCATCTC 
CTCTACTATG CAGAATTTTT GTTTCTGGGA CTGTTCCTCT TGGAGATGTC CCTGAAGATG 
TATGGCATGG GACCTCGACT TTATTTTCAC TCTTCATTCA ACTGTTTTGA TTTTGGGGTC 
ACAGTGGGCA GCATCTTTGA AGTGGTCTGG GCAATCTTCA GACCTGGCAC ATCTTTTGGA 
ATCAGTGTCT TACGGGCTCT CCGGCTTCTA AGAATATTTA AAATAACCAA GTATTGGGCA 
TCTCTGCGAA ATTTGGTGGT CTCCTTGATG AGCTCTATGA AGTCTATCAT CAGTTTGCTC 
TTCCTCCTCT TCCTCTTCAT CGTTGTCTTT GCTCTCCTAG GAATGCAGCT GTTTGGAGGC 
AGGTTTAACT TTAATGATGG GACTCCTTCA GCAAACTTTG ATACCTTCCC TGCAGCCATC 
ATGACTGTAT TCCAGATCCT AACAGGTGAG GACTGGAATG AGGTGATGTA CAATGGGATC 
CGTTCCCAGG GTGGAGTTAG CTCAGGCATG TGGTCAGCTA TCTATTTCAT TGTGCTCACC 
TTGTTTGGAA ACTACACACT GCTGAATGTG TTCTTGGCCA TTGCTGTGGA CAATCTGGCC 
AATGCCCAAG AGCTGACCAA GGATGAACAA GAAGAAGAGG AGGCTTTTAA CCAGAAACAT 
GCATTGCAGA AGGCCAAGGA GGTCAGCCCG ATGTCTGCAC CCAACATGCC TTCTATCGAA 
AGAGACAGAA GGAGAAGACA CCACATGTCG ATGTGGGAGC CACGCAGCAG CCACCTGAGG 
GAGCGGAGGC GCCGGCACCA CATGTCGGTG TGGGAGCAGC GCACCAGCCA GCTGAGAAGG 
CACATGCAGA TGTCCAGCCA GGAGGCCCTC AATAAAGAGG AGGCCCCACC GATGAACCCC 
CTCAACCCCC TCAACCCGCT GAGCCCCCTC AACCCGCTCA ACGCCCACCC CAGCCTCTAC 
CGGAGACCTA GGCCTATTGA GGGCCTGGGC CTGGGCCTGG AGAAGTGTGA GGAGGAGCGC 
ATCATCAGCC GCGGGGGGTC CCTCAAGGGG GACGGAGGGG ACCTGTCGAG TGCCCTGGAC 
AACCAGAGGA GCCCTTTGTC CCTGGGCAAA CGGGAGCCAC CATGGCTGGC CAGGCCCTGT 
CATGGGAACT GTGATCCGAC CCAGCAGGAG GCTGGGGGAG GGGAGACCGT GGTGACCTTC 
GAGGACCGGG CCAGGCACAG GCAGAGCCAG CGGCGCAGCC GGCACCGTCG TGTCAGGACG 
GAAGGCAAGG AGCTCTCCTC CACCTCCCGG AGCAGGTCTG TCAGCCAGGA GCGGAGTCTG 
GATGAGGGGG TGCTCACCGA CGGGGAGAAG GAGCGAGAGC CCAGGGGCGG CCATGCAGGC 
AAAGAACCCA CCATCCAAGA AGAAGACAGA GGCCAAGACT TAAGGAGGAC GAACAGTCTG 
ATGGTGCCCA GAGGCTCTGG GCTGGCAGGA GCCCTTGATG AGGCCAACAC ACCCCTAGTC 
CTGCCCCACC ACGATCTGGA AGTGGGGAAG GACACGGTGC TGACGGAGCA GGAGCCGGAG 
GGCAGCAGCG AGCAGGCCCT GCTGGGGGAT GTGCAGTTGG ACATTGGCAG GGTCATCAGC 
CAGAGTGAGC CTGACCTCTC CTGCGTCACA GCCAACACAG ACAAGGCCAC CACCGACAGC 
ACCAGCGTCA CCGTCGCCAT CCCTGATGTG GGCCCCTTGA TGGACTCAAC TGTGATTCAC 
ATTAGCAACA AAACTGATGG AGAAGCCAGT CCCTTGAAGG AGGCTGAGAT CAAAGAGGAG 
GAGGAGGAAA TGGAGAAGAA GCAGCAGAAG AAGGAGAAGC GTGAGACGGG CAAAGCCATG 
GTGCCCCACA GCTCTATGTT CATCTTCAGC ACCACCAACC CGATTCGCAG GGCCTGCCAC 
TACATCGTGA ACCTGCGCTA CTTTGAGATG TGCATTCTCC TGGTGATCGC CGCCAGCAGC 
ATTGCCCTGG CAGCAGAGGA CCCCGTCCTG ACCAACTCGG AGCGCAACAA AGTGGGTTCA 
CGGGAGCCGT TTGCCAACCT CCGTAATGCC CTGGTCCTGA GGTATTTTGA CTATGTTTTC 
ACTGGCGTGT TCACCTTTGA GATGGTTATA AAGATGATAG ACCAGGGCTT GATCCTACAG 
GATGGGTCCT ACTTCCGAGA CCTGTGGAAC ATCCTGGACT TTGTGGTGGT GGTTGGTGCA 
TTGGTGGCCT TTGCTTTGGC GAACGCTTTG GGAACAAACA AGGGGCGAGA CATCAAGACC 
ATCAAATCCC TGAGGGTGCT CCGAGTTCTG AGGCCACTGA AGACCATCAA GCGCTTGCCC 
AAACTCAAGG CTGTCTTTGA CTGTGTGGTG ACCTCCTTGA AGAATGTCTT CAACATACTC 
ATCGTCTATA AGCTCTTCAT GTTCATCTTT GCTGTCATTG CGGTTCAGCT CTTCAAGGGA 
AAATTCTTTT ATTGCACAGA CAGTTCCAAG GACACAGAGA AGGAGTGCAT AGGCAACTAC 
GTAGATCATG AAAAAAACAA GATGGAGGTG AAAGGCCGGG AATGGAAGCG CCATGAATTC 
CACTACGACA ACATTATCTG GGCCCTGCTG ACCCTCTTCA CCGTCTCCAC GGGGGAAGGA 
TGGCCTCAAG TCTTACAGCA TTCTGTCGAT GTGACAGAGG AAGACCGAGG ACCAAGCCGC 
AGCAACCGCA TGGAGATGTC CATCTTCTAT GTGGTCTACT TTGTGGTCTT CCCTTTCTTC 
TTTGTCAATA TCTTTGTGGC TCTGATCATC ATCACCTTCC AGGAACAAGG GGACAAGATG 
ATGGAGGAAT GCAGCCTGGA GAAGAACGAG AGGGCGTGCA TTGACTTCGC CATCAGTGCC 
AAACCTCTCA CCCGCTACAT GCCACAGAAC AGGCACACCT TCCAGTACCG CGTCTGGCAC 
TTCGTGGTGT CTCCGTCCTT TGAGTACACC ATCATGGCCA TGATCGCCCT GAACACCGTC 
GTGCTGATGA TGAAGTACTA CTCTGCTCCC TGTACCTATG AACTGGCCCT GAAGTACCTG 
AATATTGCCT TCACCATGGT GTTTTCCCTG GAATGTGTCC TAAAGATCAT CGCTTTTGGC 
TTCTTGAACT ACTTTCGAGA CACCTGGAAC ATCTTTGACT TCATCACTGT GATTGGCAGT 
ATCACAGAAA TCATACTGAC AGACAGCAAG CTGGTAAACA CCAGTGGTTT CAACATGAGC 
TTTCTGAAGC TCTTCCGTGC CGCCCGCCTC ATCAAGCTTC TGCGGCAGGG CTACACCATC 
CGTATTTTAC TCTGGACCTT CGTGCAGTCC TTTAAGGCCC TCCCCTACGT CTGCCTTTTG 
ATTGCCATGC TTTTCTTCAT CTATGCCATC ATTGGGATGC AGGTATTTGG AAACATAAAA 
TTAGATGAGG AGAGTCACAT CAACCGGCAC AACAACTTCC GGAGTTTCTT TGGGTCCCTC 
ATGTTACTCT TCAGGAGTGC CACGGGAGAG GCCTGGCAGG AGATAATGCT GTCGTGCCTC 
GGGGAGAAAG GCTGCGAGCC CGACACCACT GCGCCATCGG GGCAGAACGA GAATGAGCGC 
TGTGGCACCG ATCTGGCCTA TGTTTACTTT GTCTCCTTCA TCTTCTTCTG CTCTTTCTTG 
ATGCTCAACT TGTTTGTGGC CGTCATCATG GACAACTTCG AGTACCTGAC TCGGGACTCC 
TCCATCTTAG GGCCCCACCA CTTGGATGAG TTTGTCCGCG TCTGGGCAGA GTACGACCGA 
GCTGCATGTG GCCGCATCCA TTATACTGAG ATGTATGAAA TGCTGACTCT CATGTCACCA 
CCGCTAGGCC TCGGCAAGAG ATGTCCCTCC AAAGTGGCAT ATAAGAGGTT GGTCCTAATG 
AATATGCCAG TAGCTGAGGA CATGACAGTC CACTTCACCT CCACACTGAT GGCTCTGATC 
CGGACAGCCC TGGACATCAA AATCGCCAAA GGTGGTGCAG ACAGGCAGCA GCTAGACTCG 
GAGCTGCAAA AGGAGACCCT GGCCATCTGG CCTCACCTGT CCCAGAAGAT GCTAGACCTG 
CTTGTGCCCA TGCCCAAAGC CTCTGACCTG ACTGTGGGCA AAATCTATGC AGCAATGATG 
ATCATGGACT ACTATAAACA GAGTAAGGTG AAGAAACAGA GGCAGCAGCT GGAGGAACAG 
AAAAATGCAC CCATGTTCCA GCGAATGGAG CCCTCATCTC TGCCTCAGGA GATCATTGCT 
AATGCCAAAG CCCTGCCCTA TCTCCAGCAG GACCCCGTTT CAGGCCTGAG TGGCCGTAGT 
GGATACCCTT CGATGAGTCC ACTCTCCCCC CAGGAAATAT TCCAATTGGC TTGTATGGAT 
CCAGCCGATG ATGGACAGTT CCAGGAACAT CAGTCTCTGG TGGTGACAGA TACTAGCTCC 
ATGAGACGTT CATTTTCCAC TATTCGGGAT AAGCGTTCAA ATTCCTCCTG GTTGGAGGAA 
TTCTCCATGG AGAGAAGCAG TGAAAACACC TACAAGTCAC GTCGTCGGAG TTACCACTCC 
TCCCTGAGAC TGTCAGCTCA TCGCCTGAAT TCTGACTCAG GCCACAAGTC TGACACCCAC 
CGCTCAGGGG GTAGAGAGCG GGGACGATCG AAAGAGCGAA AGCATCTTCT CTCTCCTGAT 
GTCTCCCGCT GCAACTCAGA GGAGCGAGGG ACCCAGGCCG ACTGGGAGTC CCCAGAGCGC 
CAACAGTCCA GGTCTCCCAG TGAGGCCAGG TCACAGACCC CCAACAGACA GGGCACGGGC 
TCCCTGAGCG AGAGCTCCAT CCCCTCCATC TCTGACACCA GCACCCCAAG ACGAAGTCGC 
CGGCAGCTCC CACCCGTCCC ACCAAAGCCT CGGCCCCTCC TCTCCTACAG CTCCCTGATT 
CGACACTCCG GGAGCATCTC CCCGCCGGCC GACGGAAGCG AGAGGGGCTC CCCGCTGACC 
CCCCAGGCGC TGGAGAGCAC GGAGGCCGGC CTGGCGGAGC CCTCGGGCCC CCGGCACGGG 
CACGGGCAGG GGCACGGGCA GGGGCACGGG CAGCAGAGCC AGCACTGCTC CCCGCAGCGC 
TACATCTCCG AGCCCTACCT GGCCCTGCAC GAAGACTCCC ACGCGTCGGA CTGTGGCGAG 
GAGGAGACGC TCACCTTCGA GGCCGCCGTG GCCACCAGCC TGGGCCGCTC CAACACCATC 
GGCTCGGCCC CGCCGCTGCG GCACAGCTGG CAGATGCCCA ACGGGCACTA TCGGCGGCGG 
CGGCGCGGGG GGCCGGGGCC GGGCGTGATG TGCGGGGCGG TCAGCGACCT CCTGAGTGAC 
ACGGAAGAAG ACGACAAGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622451.1
CDS	85..7428
Translation	MEEQCSGMVPEMRGPSPGRRLPEPAPCPAAAGSPEPWAAGRRRHVLALPEEEAWRPQQCSLLEADASDEVGMLPASPRAAASGVDNFFPVQEGEGAGWEGGAALDAGAGPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFTNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLGLGLEKCEEERIISRGGSLKGDGGDLSSALDNQRSPLSLGKREPPWLARPCHGNCDPTQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKELSSTSRSRSVSQERSLDEGVLTDGEKEREPRGGHAGKEPTIQEEDRGQDLRRTNSLMVPRGSGLAGALDEANTPLVLPHHDLEVGKDTVLTEQEPEGSSEQALLGDVQLDIGRVISQSEPDLSCVTANTDKATTDSTSVTVAIPDVGPLMDSTVIHISNKTDGEASPLKEAEIKEEEEEMEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVGSREPFANLRNALVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEHQSLVVTDTSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERQQSRSPSEARSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHSGSISPPADGSERGSPLTPQALESTEAGLAEPSGPRHGHGQGHGQGHGQQSQHCSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGVMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_005622394.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622394.2

ORF Insert Sequence:

ATGGAAGAGC AATGTTCTGG GATGGTGCCC GAGATGCGTG GACCGAGCCC AGGCCGCAGG 
CTGCCCGAGC CCGCCCCCTG CCCCGCCGCG GCCGGGAGCC CTGAGCCCTG GGCCGCGGGC 
CGCCGCCGCC ACGTCCTGGC CCTGCCTGAG GAGGAGGCAT GGAGGCCCCA GCAGTGCTCC 
CTGCTCGAGG CCGACGCCTC GGACGAGGTG GGCATGCTGC CGGCCTCCCC GCGCGCCGCC 
GCCTCCGGCG TCGACAACTT CTTCCCGGTG CAGGAGGGCG AGGGCGCGGG CTGGGAGGGC 
GGTGCGGCCC TGGACGCCGG CGCCGGCCCC TTCCTCCCGG TGAGCCCCGA GGTGATGAAG 
CGGCGGCGCG GGGGCCTCAT CGAGCAGCGC GACATCATCA AGGCCCACGA GGCGCACAAG 
ATGCAGAGCA CCCCGCAGGC GCGGCGCAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG 
GTGGTCGGCA GGCCGGGGTC GGGCGATGGA GACTCGGACC AGAGCAGGAA CCGGCAAGGA 
ACCCCGGTGC CGGCCTCGGG GCCGGCGGCC GCCTACAAGC AGTCGAAAGC GCAGAGGGCG 
CGGACTATGG CTTTGTACAA CCCCATTCCC GTCCGGCAGA ACTGTTTCAC CGTCAACAGA 
TCCCTGTTCA TCTTCGGAGA AGATAACATT GTCAGGAAAT ACGCCAAGAA GCTCATCGAC 
TGGCCGCCGT TTGAGTACAT GATTTTGGCA ACCATCATTG CCAACTGCAT TGTCTTGGCC 
CTGGAACAGC ATCTTCCTGA GGATGACAAG ACCCCGATGT CCCGCAGACT GGAGAAGACA 
GAGCCGTATT TCATTGGGAT CTTTTGCTTT GAAGCCGGTA TCAAAATTGT GGCCCTGGGG 
TTCATCTTTC ATAAGGGCTC GTACCTCCGC AATGGCTGGA ATGTCATGGA CTTCATCGTG 
GTCCTCAGTG GCATCCTGGC CACTGCGGGG ACTCACTTCA ACACACACGT GGACCTGAGG 
ACCCTCCGGG CTGTGCGTGT CCTGAGACCC CTGAAGCTCG TGTCAGGGAT TCCTAGCCTG 
CAGATCGTGT TGAAGTCTAT CATGAAGGCC ATGGTGCCTC TTCTGCAAAT TGGTCTCCTG 
CTCTTCTTTG CCATCTTGAT GTTTGCTATC ATTGGCTTAG AGTTCTACAG TGGGAAGTTG 
CACCGAGCAT GTTTCACGAA CAATTCAGGT ATTCTGGAAG GATTTGACCC CCCTCACCCA 
TGTGGTGTGC AGGGCTGCCC AGCTGGCTAC GAATGCCGGG ACTGGATTGG CCCCAATGAT 
GGGATCACCC AGTTTGATAA CATCCTTTTT GCTGTGCTGA CTGTCTTCCA ATGCATCACC 
ATGGAAGGAT GGACCACCGT GCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC 
TGGCTGTACT TCATCCCCCT CATCATCATT GGATCCTTCT TTGTTCTCAA CCTAGTTCTG 
GGAGTGCTTT CCGGGGAGTT TGCCAAAGAG AGAGAGAGAG TGGAGAACCG GAGAGCTTTC 
ATGAAACTGC GGCGGCAGCA GCAGATTGAG CGGGAGCTGA ACGGGTACCG CGCCTGGATA 
GACAAAGCAG AGGAAGTCAT GCTTGCTGAA GAAAATAAAA ATGCTGGGAC GTCAGCCTTA 
GAAGTGCTTC GAAGGGCAAC CATCAAAAGG AGCCGGACAG AGGCCATGAC TCGAGACTCC 
AGTGATGAAC ACTGTGTCGA TATCTCCTCT GTGGGCACAC CTCTCGCCCG AGCCAGCATC 
AAAAGTGCAA AGGTGGATGG GGCCTCCTAT TTCCGGCACA AAGAAAGGCT TCTGCGCATC 
TCTATCCGCC ACATGGTGAA ATCCCAGGTG TTTTACTGGA TTGTGTTGAG CCTTGTGGCT 
CTCAACACTG CCTGTGTGGC CATCGTCCAT CATAACCAGC CCCAGTGGCT CACCCATCTC 
CTCTACTATG CAGAATTTTT GTTTCTGGGA CTGTTCCTCT TGGAGATGTC CCTGAAGATG 
TATGGCATGG GACCTCGACT TTATTTTCAC TCTTCATTCA ACTGTTTTGA TTTTGGGGTC 
ACAGTGGGCA GCATCTTTGA AGTGGTCTGG GCAATCTTCA GACCTGGCAC ATCTTTTGGA 
ATCAGTGTCT TACGGGCTCT CCGGCTTCTA AGAATATTTA AAATAACCAA GTATTGGGCA 
TCTCTGCGAA ATTTGGTGGT CTCCTTGATG AGCTCTATGA AGTCTATCAT CAGTTTGCTC 
TTCCTCCTCT TCCTCTTCAT CGTTGTCTTT GCTCTCCTAG GAATGCAGCT GTTTGGAGGC 
AGGTTTAACT TTAATGATGG GACTCCTTCA GCAAACTTTG ATACCTTCCC TGCAGCCATC 
ATGACTGTAT TCCAGATCCT AACAGGTGAG GACTGGAATG AGGTGATGTA CAATGGGATC 
CGTTCCCAGG GTGGAGTTAG CTCAGGCATG TGGTCAGCTA TCTATTTCAT TGTGCTCACC 
TTGTTTGGAA ACTACACACT GCTGAATGTG TTCTTGGCCA TTGCTGTGGA CAATCTGGCC 
AATGCCCAAG AGCTGACCAA GGATGAACAA GAAGAAGAGG AGGCTTTTAA CCAGAAACAT 
GCATTGCAGA AGGCCAAGGA GGTCAGCCCG ATGTCTGCAC CCAACATGCC TTCTATCGAA 
AGAGACAGAA GGAGAAGACA CCACATGTCG ATGTGGGAGC CACGCAGCAG CCACCTGAGG 
GAGCGGAGGC GCCGGCACCA CATGTCGGTG TGGGAGCAGC GCACCAGCCA GCTGAGAAGG 
CACATGCAGA TGTCCAGCCA GGAGGCCCTC AATAAAGAGG AGGCCCCACC GATGAACCCC 
CTCAACCCCC TCAACCCGCT GAGCCCCCTC AACCCGCTCA ACGCCCACCC CAGCCTCTAC 
CGGAGACCTA GGCCTATTGA GGGCCTGGGC CTGGGCCTGG AGAAGTGTGA GGAGGAGCGC 
ATCATCAGCC GCGGGGGGTC CCTCAAGGGG GACGGAGGGG ACCTGTCGAG TGCCCTGGAC 
AACCAGAGGA GCCCTTTGTC CCTGGGCAAA CGGGAGCCAC CATGGCTGGC CAGGCCCTGT 
CATGGGAACT GTGATCCGAC CCAGCAGGAG GCTGGGGGAG GGGAGACCGT GGTGACCTTC 
GAGGACCGGG CCAGGCACAG GCAGAGCCAG CGGCGCAGCC GGCACCGTCG TGTCAGGACG 
GAAGGCAAGG AGCTCTCCTC CACCTCCCGG AGCAGGTCTG TCAGCCAGGA GCGGAGTCTG 
GATGAGGGGG TGCTCACCGA CGGGGAGAAG GAGCGAGAGC CCAGGGGCGG CCATGCAGGC 
AAAGAACCCA CCATCCAAGA AGAAGACAGA GGCCAAGACT TAAGGAGGAC GAACAGTCTG 
ATGGTGCCCA GAGGCTCTGG GCTGGCAGGA GCCCTTGATG AGGCCAACAC ACCCCTAGTC 
CTGCCCCACC ACGATCTGGA AGTGGGGAAG GACACGGTGC TGACGGAGCA GGAGCCGGAG 
GGCAGCAGCG AGCAGGCCCT GCTGGGGGAT GTGCAGTTGG ACATTGGCAG GGTCATCAGC 
CAGAGTGAGC CTGACCTCTC CTGCGTCACA GCCAACACAG ACAAGGCCAC CACCGACAGC 
ACCAGCGTCA CCGTCGCCAT CCCTGATGTG GGCCCCTTGA TGGACTCAAC TGTGATTCAC 
ATTAGCAACA AAACTGATGG AGAAGCCAGT CCCTTGAAGG AGGCTGAGAT CAAAGAGGAG 
GAGGAGGAAA TGGAGAAGAA GCAGCAGAAG AAGGAGAAGC GTGAGACGGG CAAAGCCATG 
GTGCCCCACA GCTCTATGTT CATCTTCAGC ACCACCAACC CGATTCGCAG GGCCTGCCAC 
TACATCGTGA ACCTGCGCTA CTTTGAGATG TGCATTCTCC TGGTGATCGC CGCCAGCAGC 
ATTGCCCTGG CAGCAGAGGA CCCCGTCCTG ACCAACTCGG AGCGCAACAA AGTGGGTTCA 
CGGGAGCCGT TTGCCAACCT CCGTAATGCC CTGGTCCTGA GGTATTTTGA CTATGTTTTC 
ACTGGCGTGT TCACCTTTGA GATGGTTATA AAGATGATAG ACCAGGGCTT GATCCTACAG 
GATGGGTCCT ACTTCCGAGA CCTGTGGAAC ATCCTGGACT TTGTGGTGGT GGTTGGTGCA 
TTGGTGGCCT TTGCTTTGGC GAACGCTTTG GGAACAAACA AGGGGCGAGA CATCAAGACC 
ATCAAATCCC TGAGGGTGCT CCGAGTTCTG AGGCCACTGA AGACCATCAA GCGCTTGCCC 
AAACTCAAGG CTGTCTTTGA CTGTGTGGTG ACCTCCTTGA AGAATGTCTT CAACATACTC 
ATCGTCTATA AGCTCTTCAT GTTCATCTTT GCTGTCATTG CGGTTCAGCT CTTCAAGGGA 
AAATTCTTTT ATTGCACAGA CAGTTCCAAG GACACAGAGA AGGAGTGCAT AGGCAACTAC 
GTAGATCATG AAAAAAACAA GATGGAGGTG AAAGGCCGGG AATGGAAGCG CCATGAATTC 
CACTACGACA ACATTATCTG GGCCCTGCTG ACCCTCTTCA CCGTCTCCAC GGGGGAAGGA 
TGGCCTCAAG TCTTACAGCA TTCTGTCGAT GTGACAGAGG AAGACCGAGG ACCAAGCCGC 
AGCAACCGCA TGGAGATGTC CATCTTCTAT GTGGTCTACT TTGTGGTCTT CCCTTTCTTC 
TTTGTCAATA TCTTTGTGGC TCTGATCATC ATCACCTTCC AGGAACAAGG GGACAAGATG 
ATGGAGGAAT GCAGCCTGGA GAAGAACGAG AGGGCGTGCA TTGACTTCGC CATCAGTGCC 
AAACCTCTCA CCCGCTACAT GCCACAGAAC AGGCACACCT TCCAGTACCG CGTCTGGCAC 
TTCGTGGTGT CTCCGTCCTT TGAGTACACC ATCATGGCCA TGATCGCCCT GAACACCGTC 
GTGCTGATGA TGAAGTACTA CTCTGCTCCC TGTACCTATG AACTGGCCCT GAAGTACCTG 
AATATTGCCT TCACCATGGT GTTTTCCCTG GAATGTGTCC TAAAGATCAT CGCTTTTGGC 
TTCTTGAACT ACTTTCGAGA CACCTGGAAC ATCTTTGACT TCATCACTGT GATTGGCAGT 
ATCACAGAAA TCATACTGAC AGACAGCAAG CTGGTAAACA CCAGTGGTTT CAACATGAGC 
TTTCTGAAGC TCTTCCGTGC CGCCCGCCTC ATCAAGCTTC TGCGGCAGGG CTACACCATC 
CGTATTTTAC TCTGGACCTT CGTGCAGTCC TTTAAGGCCC TCCCCTACGT CTGCCTTTTG 
ATTGCCATGC TTTTCTTCAT CTATGCCATC ATTGGGATGC AGGTATTTGG AAACATAAAA 
TTAGATGAGG AGAGTCACAT CAACCGGCAC AACAACTTCC GGAGTTTCTT TGGGTCCCTC 
ATGTTACTCT TCAGGAGTGC CACGGGAGAG GCCTGGCAGG AGATAATGCT GTCGTGCCTC 
GGGGAGAAAG GCTGCGAGCC CGACACCACT GCGCCATCGG GGCAGAACGA GAATGAGCGC 
TGTGGCACCG ATCTGGCCTA TGTTTACTTT GTCTCCTTCA TCTTCTTCTG CTCTTTCTTG 
ATGCTCAACT TGTTTGTGGC CGTCATCATG GACAACTTCG AGTACCTGAC TCGGGACTCC 
TCCATCTTAG GGCCCCACCA CTTGGATGAG TTTGTCCGCG TCTGGGCAGA GTACGACCGA 
GCTGCATGTG GCCGCATCCA TTATACTGAG ATGTATGAAA TGCTGACTCT CATGTCACCA 
CCGCTAGGCC TCGGCAAGAG ATGTCCCTCC AAAGTGGCAT ATAAGAGGTT GGTCCTAATG 
AATATGCCAG TAGCTGAGGA CATGACAGTC CACTTCACCT CCACACTGAT GGCTCTGATC 
CGGACAGCCC TGGACATCAA AATCGCCAAA GGTGGTGCAG ACAGGCAGCA GCTAGACTCG 
GAGCTGCAAA AGGAGACCCT GGCCATCTGG CCTCACCTGT CCCAGAAGAT GCTAGACCTG 
CTTGTGCCCA TGCCCAAAGC CTCTGACCTG ACTGTGGGCA AAATCTATGC AGCAATGATG 
ATCATGGACT ACTATAAACA GAGTAAGGTG AAGAAACAGA GGCAGCAGCT GGAGGAACAG 
AAAAATGCAC CCATGTTCCA GCGAATGGAG CCCTCATCTC TGCCTCAGGA GATCATTGCT 
AATGCCAAAG CCCTGCCCTA TCTCCAGCAG GACCCCGTTT CAGGCCTGAG TGGCCGTAGT 
GGATACCCTT CGATGAGTCC ACTCTCCCCC CAGGAAATAT TCCAATTGGC TTGTATGGAT 
CCAGCCGATG ATGGACAGTT CCAGGAACAT CAGTCTCTGG TGGTGACAGA TACTAGCTCC 
ATGAGACGTT CATTTTCCAC TATTCGGGAT AAGCGTTCAA ATTCCTCCTG GTTGGAGGAA 
TTCTCCATGG AGAGAAGCAG TGAAAACACC TACAAGTCAC GTCGTCGGAG TTACCACTCC 
TCCCTGAGAC TGTCAGCTCA TCGCCTGAAT TCTGACTCAG GCCACAAGTC TGACACCCAC 
CGCTCAGGGG GTAGAGAGCG GGGACGATCG AAAGAGCGAA AGCATCTTCT CTCTCCTGAT 
GTCTCCCGCT GCAACTCAGA GGAGCGAGGG ACCCAGGCCG ACTGGGAGTC CCCAGAGCGC 
CAACAGTCCA GGTCTCCCAG TGAGGCCAGG TCACAGACCC CCAACAGACA GGGCACGGGC 
TCCCTGAGCG AGAGCTCCAT CCCCTCCATC TCTGACACCA GCACCCCAAG ACGAAGTCGC 
CGGCAGCTCC CACCCGTCCC ACCAAAGCCT CGGCCCCTCC TCTCCTACAG CTCCCTGATT 
CGACACTCCG GGAGCATCTC CCCGCCGGCC GACGGAAGCG AGAGGGGCTC CCCGCTGACC 
CCCCAGGCGC TGGAGAGCAC GGAGGCCGGC CTGGCGGAGC CCTCGGGCCC CCGGCACGGG 
CACGGGCAGG GGCACGGGCA GGGGCACGGG CAGCAGAGCC AGCACTGCTC CCCGCAGCGC 
TACATCTCCG AGCCCTACCT GGCCCTGCAC GAAGACTCCC ACGCGTCGGA CTGTGGCGAG 
GAGGAGACGC TCACCTTCGA GGCCGCCGTG GCCACCAGCC TGGGCCGCTC CAACACCATC 
GGCTCGGCCC CGCCGCTGCG GCACAGCTGG CAGATGCCCA ACGGGCACTA TCGGCGGCGG 
CGGCGCGGGG GGCCGGGGCC GGGCGTGATG TGCGGGGCGG TCAGCGACCT CCTGAGTGAC 
ACGGAAGAAG ACGACAAGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_003434970.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003435018.2 Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OCe09118
Clone ID Related Accession (Same CDS sequence)	XM_003434970.3
Accession Version	XM_003434970.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003434970.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGC AATGTTCTGG GATGGTGCCC GAGATGCGTG GACCGAGCCC AGGCCGCAGG 
CTGCCCGAGC CCGCCCCCTG CCCCGCCGCG GCCGGGAGCC CTGAGCCCTG GGCCGCGGGC 
CGCCGCCGCC ACGTCCTGGC CCTGCCTGAG GAGGAGGCAT GGAGGCCCCA GCAGTGCTCC 
CTGCTCGAGG CCGACGCCTC GGACGAGGTG GGCATGCTGC CGGCCTCCCC GCGCGCCGCC 
GCCTCCGGCG TCGACAACTT CTTCCCGGTG CAGGAGGGCG AGGGCGCGGG CTGGGAGGGC 
GGTGCGGCCC TGGACGCCGG CGCCGGCCCC TTCCTCCCGG TGAGCCCCGA GGTGATGAAG 
CGGCGGCGCG GGGGCCTCAT CGAGCAGCGC GACATCATCA AGGCCCACGA GGCGCACAAG 
ATGCAGAGCA CCCCGCAGGC GCGGCGCAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG 
GTGGTCGGCA GGCCGGGGTC GGGCGATGGA GACTCGGACC AGAGCAGGAA CCGGCAAGGA 
ACCCCGGTGC CGGCCTCGGG GCCGGCGGCC GCCTACAAGC AGTCGAAAGC GCAGAGGGCG 
CGGACTATGG CTTTGTACAA CCCCATTCCC GTCCGGCAGA ACTGTTTCAC CGTCAACAGA 
TCCCTGTTCA TCTTCGGAGA AGATAACATT GTCAGGAAAT ACGCCAAGAA GCTCATCGAC 
TGGCCGCCGT TTGAGTACAT GATTTTGGCA ACCATCATTG CCAACTGCAT TGTCTTGGCC 
CTGGAACAGC ATCTTCCTGA GGATGACAAG ACCCCGATGT CCCGCAGACT GGAGAAGACA 
GAGCCGTATT TCATTGGGAT CTTTTGCTTT GAAGCCGGTA TCAAAATTGT GGCCCTGGGG 
TTCATCTTTC ATAAGGGCTC GTACCTCCGC AATGGCTGGA ATGTCATGGA CTTCATCGTG 
GTCCTCAGTG GCATCCTGGC CACTGCGGGG ACTCACTTCA ACACACACGT GGACCTGAGG 
ACCCTCCGGG CTGTGCGTGT CCTGAGACCC CTGAAGCTCG TGTCAGGGAT TCCTAGCCTG 
CAGATCGTGT TGAAGTCTAT CATGAAGGCC ATGGTGCCTC TTCTGCAAAT TGGTCTCCTG 
CTCTTCTTTG CCATCTTGAT GTTTGCTATC ATTGGCTTAG AGTTCTACAG TGGGAAGTTG 
CACCGAGCAT GTTTCACGAA CAATTCAGGT ATTCTGGAAG GATTTGACCC CCCTCACCCA 
TGTGGTGTGC AGGGCTGCCC AGCTGGCTAC GAATGCCGGG ACTGGATTGG CCCCAATGAT 
GGGATCACCC AGTTTGATAA CATCCTTTTT GCTGTGCTGA CTGTCTTCCA ATGCATCACC 
ATGGAAGGAT GGACCACCGT GCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC 
TGGCTGTACT TCATCCCCCT CATCATCATT GGATCCTTCT TTGTTCTCAA CCTAGTTCTG 
GGAGTGCTTT CCGGGGAGTT TGCCAAAGAG AGAGAGAGAG TGGAGAACCG GAGAGCTTTC 
ATGAAACTGC GGCGGCAGCA GCAGATTGAG CGGGAGCTGA ACGGGTACCG CGCCTGGATA 
GACAAAGCAG AGGAAGTCAT GCTTGCTGAA GAAAATAAAA ATGCTGGGAC GTCAGCCTTA 
GAAGTGCTTC GAAGGGCAAC CATCAAAAGG AGCCGGACAG AGGCCATGAC TCGAGACTCC 
AGTGATGAAC ACTGTGTCGA TATCTCCTCT GTGGGCACAC CTCTCGCCCG AGCCAGCATC 
AAAAGTGCAA AGGTGGATGG GGCCTCCTAT TTCCGGCACA AAGAAAGGCT TCTGCGCATC 
TCTATCCGCC ACATGGTGAA ATCCCAGGTG TTTTACTGGA TTGTGTTGAG CCTTGTGGCT 
CTCAACACTG CCTGTGTGGC CATCGTCCAT CATAACCAGC CCCAGTGGCT CACCCATCTC 
CTCTACTATG CAGAATTTTT GTTTCTGGGA CTGTTCCTCT TGGAGATGTC CCTGAAGATG 
TATGGCATGG GACCTCGACT TTATTTTCAC TCTTCATTCA ACTGTTTTGA TTTTGGGGTC 
ACAGTGGGCA GCATCTTTGA AGTGGTCTGG GCAATCTTCA GACCTGGCAC ATCTTTTGGA 
ATCAGTGTCT TACGGGCTCT CCGGCTTCTA AGAATATTTA AAATAACCAA GTATTGGGCA 
TCTCTGCGAA ATTTGGTGGT CTCCTTGATG AGCTCTATGA AGTCTATCAT CAGTTTGCTC 
TTCCTCCTCT TCCTCTTCAT CGTTGTCTTT GCTCTCCTAG GAATGCAGCT GTTTGGAGGC 
AGGTTTAACT TTAATGATGG GACTCCTTCA GCAAACTTTG ATACCTTCCC TGCAGCCATC 
ATGACTGTAT TCCAGATCCT AACAGGTGAG GACTGGAATG AGGTGATGTA CAATGGGATC 
CGTTCCCAGG GTGGAGTTAG CTCAGGCATG TGGTCAGCTA TCTATTTCAT TGTGCTCACC 
TTGTTTGGAA ACTACACACT GCTGAATGTG TTCTTGGCCA TTGCTGTGGA CAATCTGGCC 
AATGCCCAAG AGCTGACCAA GGATGAACAA GAAGAAGAGG AGGCTTTTAA CCAGAAACAT 
GCATTGCAGA AGGCCAAGGA GGTCAGCCCG ATGTCTGCAC CCAACATGCC TTCTATCGAA 
AGAGACAGAA GGAGAAGACA CCACATGTCG ATGTGGGAGC CACGCAGCAG CCACCTGAGG 
GAGCGGAGGC GCCGGCACCA CATGTCGGTG TGGGAGCAGC GCACCAGCCA GCTGAGAAGG 
CACATGCAGA TGTCCAGCCA GGAGGCCCTC AATAAAGAGG AGGCCCCACC GATGAACCCC 
CTCAACCCCC TCAACCCGCT GAGCCCCCTC AACCCGCTCA ACGCCCACCC CAGCCTCTAC 
CGGAGACCTA GGCCTATTGA GGGCCTGGGC CTGGGCCTGG AGAAGTGTGA GGAGGAGCGC 
ATCATCAGCC GCGGGGGGTC CCTCAAGGGG GACGGAGGGG ACCTGTCGAG TGCCCTGGAC 
AACCAGAGGA GCCCTTTGTC CCTGGGCAAA CGGGAGCCAC CATGGCTGGC CAGGCCCTGT 
CATGGGAACT GTGATCCGAC CCAGCAGGAG GCTGGGGGAG GGGAGACCGT GGTGACCTTC 
GAGGACCGGG CCAGGCACAG GCAGAGCCAG CGGCGCAGCC GGCACCGTCG TGTCAGGACG 
GAAGGCAAGG AGCTCTCCTC CACCTCCCGG AGCAGGTCTG TCAGCCAGGA GCGGAGTCTG 
GATGAGGGGG TGCTCACCGA CGGGGAGAAG GAGCGAGAGC CCAGGGGCGG CCATGCAGGC 
AAAGAACCCA CCATCCAAGA AGAAGACAGA GGCCAAGACT TAAGGAGGAC GAACAGTCTG 
ATGGTGCCCA GAGGCTCTGG GCTGGCAGGA GCCCTTGATG AGGCCAACAC ACCCCTAGTC 
CTGCCCCACC ACGATCTGGA AGTGGGGAAG GACACGGTGC TGACGGAGCA GGAGCCGGAG 
GGCAGCAGCG AGCAGGCCCT GCTGGGGGAT GTGCAGTTGG ACATTGGCAG GGTCATCAGC 
CAGAGTGAGC CTGACCTCTC CTGCGTCACA GCCAACACAG ACAAGGCCAC CACCGACAGC 
ACCAGCGTCA CCGTCGCCAT CCCTGATGTG GGCCCCTTGA TGGACTCAAC TGTGATTCAC 
ATTAGCAACA AAACTGATGG AGAAGCCAGT CCCTTGAAGG AGGCTGAGAT CAAAGAGGAG 
GAGGAGGAAA TGGAGAAGAA GCAGCAGAAG AAGGAGAAGC GTGAGACGGG CAAAGCCATG 
GTGCCCCACA GCTCTATGTT CATCTTCAGC ACCACCAACC CGATTCGCAG GGCCTGCCAC 
TACATCGTGA ACCTGCGCTA CTTTGAGATG TGCATTCTCC TGGTGATCGC CGCCAGCAGC 
ATTGCCCTGG CAGCAGAGGA CCCCGTCCTG ACCAACTCGG AGCGCAACAA AGTCCTGAGG 
TATTTTGACT ATGTTTTCAC TGGCGTGTTC ACCTTTGAGA TGGTTATAAA GATGATAGAC 
CAGGGCTTGA TCCTACAGGA TGGGTCCTAC TTCCGAGACC TGTGGAACAT CCTGGACTTT 
GTGGTGGTGG TTGGTGCATT GGTGGCCTTT GCTTTGGCGA ACGCTTTGGG AACAAACAAG 
GGGCGAGACA TCAAGACCAT CAAATCCCTG AGGGTGCTCC GAGTTCTGAG GCCACTGAAG 
ACCATCAAGC GCTTGCCCAA ACTCAAGGCT GTCTTTGACT GTGTGGTGAC CTCCTTGAAG 
AATGTCTTCA ACATACTCAT CGTCTATAAG CTCTTCATGT TCATCTTTGC TGTCATTGCG 
GTTCAGCTCT TCAAGGGAAA ATTCTTTTAT TGCACAGACA GTTCCAAGGA CACAGAGAAG 
GAGTGCATAG GCAACTACGT AGATCATGAA AAAAACAAGA TGGAGGTGAA AGGCCGGGAA 
TGGAAGCGCC ATGAATTCCA CTACGACAAC ATTATCTGGG CCCTGCTGAC CCTCTTCACC 
GTCTCCACGG GGGAAGGATG GCCTCAAGTC TTACAGCATT CTGTCGATGT GACAGAGGAA 
GACCGAGGAC CAAGCCGCAG CAACCGCATG GAGATGTCCA TCTTCTATGT GGTCTACTTT 
GTGGTCTTCC CTTTCTTCTT TGTCAATATC TTTGTGGCTC TGATCATCAT CACCTTCCAG 
GAACAAGGGG ACAAGATGAT GGAGGAATGC AGCCTGGAGA AGAACGAGAG GGCGTGCATT 
GACTTCGCCA TCAGTGCCAA ACCTCTCACC CGCTACATGC CACAGAACAG GCACACCTTC 
CAGTACCGCG TCTGGCACTT CGTGGTGTCT CCGTCCTTTG AGTACACCAT CATGGCCATG 
ATCGCCCTGA ACACCGTCGT GCTGATGATG AAGTACTACT CTGCTCCCTG TACCTATGAA 
CTGGCCCTGA AGTACCTGAA TATTGCCTTC ACCATGGTGT TTTCCCTGGA ATGTGTCCTA 
AAGATCATCG CTTTTGGCTT CTTGAACTAC TTTCGAGACA CCTGGAACAT CTTTGACTTC 
ATCACTGTGA TTGGCAGTAT CACAGAAATC ATACTGACAG ACAGCAAGCT GGTAAACACC 
AGTGGTTTCA ACATGAGCTT TCTGAAGCTC TTCCGTGCCG CCCGCCTCAT CAAGCTTCTG 
CGGCAGGGCT ACACCATCCG TATTTTACTC TGGACCTTCG TGCAGTCCTT TAAGGCCCTC 
CCCTACGTCT GCCTTTTGAT TGCCATGCTT TTCTTCATCT ATGCCATCAT TGGGATGCAG 
GTATTTGGAA ACATAAAATT AGATGAGGAG AGTCACATCA ACCGGCACAA CAACTTCCGG 
AGTTTCTTTG GGTCCCTCAT GTTACTCTTC AGGAGTGCCA CGGGAGAGGC CTGGCAGGAG 
ATAATGCTGT CGTGCCTCGG GGAGAAAGGC TGCGAGCCCG ACACCACTGC GCCATCGGGG 
CAGAACGAGA ATGAGCGCTG TGGCACCGAT CTGGCCTATG TTTACTTTGT CTCCTTCATC 
TTCTTCTGCT CTTTCTTGAT GCTCAACTTG TTTGTGGCCG TCATCATGGA CAACTTCGAG 
TACCTGACTC GGGACTCCTC CATCTTAGGG CCCCACCACT TGGATGAGTT TGTCCGCGTC 
TGGGCAGAGT ACGACCGAGC TGCATGTGGC CGCATCCATT ATACTGAGAT GTATGAAATG 
CTGACTCTCA TGTCACCACC GCTAGGCCTC GGCAAGAGAT GTCCCTCCAA AGTGGCATAT 
AAGAGGTTGG TCCTAATGAA TATGCCAGTA GCTGAGGACA TGACAGTCCA CTTCACCTCC 
ACACTGATGG CTCTGATCCG GACAGCCCTG GACATCAAAA TCGCCAAAGG TGGTGCAGAC 
AGGCAGCAGC TAGACTCGGA GCTGCAAAAG GAGACCCTGG CCATCTGGCC TCACCTGTCC 
CAGAAGATGC TAGACCTGCT TGTGCCCATG CCCAAAGCCT CTGACCTGAC TGTGGGCAAA 
ATCTATGCAG CAATGATGAT CATGGACTAC TATAAACAGA GTAAGGTGAA GAAACAGAGG 
CAGCAGCTGG AGGAACAGAA AAATGCACCC ATGTTCCAGC GAATGGAGCC CTCATCTCTG 
CCTCAGGAGA TCATTGCTAA TGCCAAAGCC CTGCCCTATC TCCAGCAGGA CCCCGTTTCA 
GGCCTGAGTG GCCGTAGTGG ATACCCTTCG ATGAGTCCAC TCTCCCCCCA GGAAATATTC 
CAATTGGCTT GTATGGATCC AGCCGATGAT GGACAGTTCC AGGAACATCA GTCTCTGGTG 
GTGACAGATA CTAGCTCCAT GAGACGTTCA TTTTCCACTA TTCGGGATAA GCGTTCAAAT 
TCCTCCTGGT TGGAGGAATT CTCCATGGAG AGAAGCAGTG AAAACACCTA CAAGTCACGT 
CGTCGGAGTT ACCACTCCTC CCTGAGACTG TCAGCTCATC GCCTGAATTC TGACTCAGGC 
CACAAGTCTG ACACCCACCG CTCAGGGGGT AGAGAGCGGG GACGATCGAA AGAGCGAAAG 
CATCTTCTCT CTCCTGATGT CTCCCGCTGC AACTCAGAGG AGCGAGGGAC CCAGGCCGAC 
TGGGAGTCCC CAGAGCGCCA ACAGTCCAGG TCTCCCAGTG AGGCCAGGTC ACAGACCCCC 
AACAGACAGG GCACGGGCTC CCTGAGCGAG AGCTCCATCC CCTCCATCTC TGACACCAGC 
ACCCCAAGAC GAAGTCGCCG GCAGCTCCCA CCCGTCCCAC CAAAGCCTCG GCCCCTCCTC 
TCCTACAGCT CCCTGATTCG ACACTCCGGG AGCATCTCCC CGCCGGCCGA CGGAAGCGAG 
AGGGGCTCCC CGCTGACCCC CCAGGCGCTG GAGAGCACGG AGGCCGGCCT GGCGGAGCCC 
TCGGGCCCCC GGCACGGGCA CGGGCAGGGG CACGGGCAGG GGCACGGGCA GCAGAGCCAG 
CACTGCTCCC CGCAGCGCTA CATCTCCGAG CCCTACCTGG CCCTGCACGA AGACTCCCAC 
GCGTCGGACT GTGGCGAGGA GGAGACGCTC ACCTTCGAGG CCGCCGTGGC CACCAGCCTG 
GGCCGCTCCA ACACCATCGG CTCGGCCCCG CCGCTGCGGC ACAGCTGGCA GATGCCCAAC 
GGGCACTATC GGCGGCGGCG GCGCGGGGGG CCGGGGCCGG GCGTGATGTG CGGGGCGGTC 
AGCGACCTCC TGAGTGACAC GGAAGAAGAC GACAAGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003435018.2
CDS	85..7386
Translation	MEEQCSGMVPEMRGPSPGRRLPEPAPCPAAAGSPEPWAAGRRRHVLALPEEEAWRPQQCSLLEADASDEVGMLPASPRAAASGVDNFFPVQEGEGAGWEGGAALDAGAGPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFTNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLGLGLEKCEEERIISRGGSLKGDGGDLSSALDNQRSPLSLGKREPPWLARPCHGNCDPTQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKELSSTSRSRSVSQERSLDEGVLTDGEKEREPRGGHAGKEPTIQEEDRGQDLRRTNSLMVPRGSGLAGALDEANTPLVLPHHDLEVGKDTVLTEQEPEGSSEQALLGDVQLDIGRVISQSEPDLSCVTANTDKATTDSTSVTVAIPDVGPLMDSTVIHISNKTDGEASPLKEAEIKEEEEEMEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEHQSLVVTDTSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERQQSRSPSEARSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHSGSISPPADGSERGSPLTPQALESTEAGLAEPSGPRHGHGQGHGQGHGQQSQHCSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGVMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_003434970.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003434970.3

ORF Insert Sequence:

ATGGAAGAGC AATGTTCTGG GATGGTGCCC GAGATGCGTG GACCGAGCCC AGGCCGCAGG 
CTGCCCGAGC CCGCCCCCTG CCCCGCCGCG GCCGGGAGCC CTGAGCCCTG GGCCGCGGGC 
CGCCGCCGCC ACGTCCTGGC CCTGCCTGAG GAGGAGGCAT GGAGGCCCCA GCAGTGCTCC 
CTGCTCGAGG CCGACGCCTC GGACGAGGTG GGCATGCTGC CGGCCTCCCC GCGCGCCGCC 
GCCTCCGGCG TCGACAACTT CTTCCCGGTG CAGGAGGGCG AGGGCGCGGG CTGGGAGGGC 
GGTGCGGCCC TGGACGCCGG CGCCGGCCCC TTCCTCCCGG TGAGCCCCGA GGTGATGAAG 
CGGCGGCGCG GGGGCCTCAT CGAGCAGCGC GACATCATCA AGGCCCACGA GGCGCACAAG 
ATGCAGAGCA CCCCGCAGGC GCGGCGCAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG 
GTGGTCGGCA GGCCGGGGTC GGGCGATGGA GACTCGGACC AGAGCAGGAA CCGGCAAGGA 
ACCCCGGTGC CGGCCTCGGG GCCGGCGGCC GCCTACAAGC AGTCGAAAGC GCAGAGGGCG 
CGGACTATGG CTTTGTACAA CCCCATTCCC GTCCGGCAGA ACTGTTTCAC CGTCAACAGA 
TCCCTGTTCA TCTTCGGAGA AGATAACATT GTCAGGAAAT ACGCCAAGAA GCTCATCGAC 
TGGCCGCCGT TTGAGTACAT GATTTTGGCA ACCATCATTG CCAACTGCAT TGTCTTGGCC 
CTGGAACAGC ATCTTCCTGA GGATGACAAG ACCCCGATGT CCCGCAGACT GGAGAAGACA 
GAGCCGTATT TCATTGGGAT CTTTTGCTTT GAAGCCGGTA TCAAAATTGT GGCCCTGGGG 
TTCATCTTTC ATAAGGGCTC GTACCTCCGC AATGGCTGGA ATGTCATGGA CTTCATCGTG 
GTCCTCAGTG GCATCCTGGC CACTGCGGGG ACTCACTTCA ACACACACGT GGACCTGAGG 
ACCCTCCGGG CTGTGCGTGT CCTGAGACCC CTGAAGCTCG TGTCAGGGAT TCCTAGCCTG 
CAGATCGTGT TGAAGTCTAT CATGAAGGCC ATGGTGCCTC TTCTGCAAAT TGGTCTCCTG 
CTCTTCTTTG CCATCTTGAT GTTTGCTATC ATTGGCTTAG AGTTCTACAG TGGGAAGTTG 
CACCGAGCAT GTTTCACGAA CAATTCAGGT ATTCTGGAAG GATTTGACCC CCCTCACCCA 
TGTGGTGTGC AGGGCTGCCC AGCTGGCTAC GAATGCCGGG ACTGGATTGG CCCCAATGAT 
GGGATCACCC AGTTTGATAA CATCCTTTTT GCTGTGCTGA CTGTCTTCCA ATGCATCACC 
ATGGAAGGAT GGACCACCGT GCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC 
TGGCTGTACT TCATCCCCCT CATCATCATT GGATCCTTCT TTGTTCTCAA CCTAGTTCTG 
GGAGTGCTTT CCGGGGAGTT TGCCAAAGAG AGAGAGAGAG TGGAGAACCG GAGAGCTTTC 
ATGAAACTGC GGCGGCAGCA GCAGATTGAG CGGGAGCTGA ACGGGTACCG CGCCTGGATA 
GACAAAGCAG AGGAAGTCAT GCTTGCTGAA GAAAATAAAA ATGCTGGGAC GTCAGCCTTA 
GAAGTGCTTC GAAGGGCAAC CATCAAAAGG AGCCGGACAG AGGCCATGAC TCGAGACTCC 
AGTGATGAAC ACTGTGTCGA TATCTCCTCT GTGGGCACAC CTCTCGCCCG AGCCAGCATC 
AAAAGTGCAA AGGTGGATGG GGCCTCCTAT TTCCGGCACA AAGAAAGGCT TCTGCGCATC 
TCTATCCGCC ACATGGTGAA ATCCCAGGTG TTTTACTGGA TTGTGTTGAG CCTTGTGGCT 
CTCAACACTG CCTGTGTGGC CATCGTCCAT CATAACCAGC CCCAGTGGCT CACCCATCTC 
CTCTACTATG CAGAATTTTT GTTTCTGGGA CTGTTCCTCT TGGAGATGTC CCTGAAGATG 
TATGGCATGG GACCTCGACT TTATTTTCAC TCTTCATTCA ACTGTTTTGA TTTTGGGGTC 
ACAGTGGGCA GCATCTTTGA AGTGGTCTGG GCAATCTTCA GACCTGGCAC ATCTTTTGGA 
ATCAGTGTCT TACGGGCTCT CCGGCTTCTA AGAATATTTA AAATAACCAA GTATTGGGCA 
TCTCTGCGAA ATTTGGTGGT CTCCTTGATG AGCTCTATGA AGTCTATCAT CAGTTTGCTC 
TTCCTCCTCT TCCTCTTCAT CGTTGTCTTT GCTCTCCTAG GAATGCAGCT GTTTGGAGGC 
AGGTTTAACT TTAATGATGG GACTCCTTCA GCAAACTTTG ATACCTTCCC TGCAGCCATC 
ATGACTGTAT TCCAGATCCT AACAGGTGAG GACTGGAATG AGGTGATGTA CAATGGGATC 
CGTTCCCAGG GTGGAGTTAG CTCAGGCATG TGGTCAGCTA TCTATTTCAT TGTGCTCACC 
TTGTTTGGAA ACTACACACT GCTGAATGTG TTCTTGGCCA TTGCTGTGGA CAATCTGGCC 
AATGCCCAAG AGCTGACCAA GGATGAACAA GAAGAAGAGG AGGCTTTTAA CCAGAAACAT 
GCATTGCAGA AGGCCAAGGA GGTCAGCCCG ATGTCTGCAC CCAACATGCC TTCTATCGAA 
AGAGACAGAA GGAGAAGACA CCACATGTCG ATGTGGGAGC CACGCAGCAG CCACCTGAGG 
GAGCGGAGGC GCCGGCACCA CATGTCGGTG TGGGAGCAGC GCACCAGCCA GCTGAGAAGG 
CACATGCAGA TGTCCAGCCA GGAGGCCCTC AATAAAGAGG AGGCCCCACC GATGAACCCC 
CTCAACCCCC TCAACCCGCT GAGCCCCCTC AACCCGCTCA ACGCCCACCC CAGCCTCTAC 
CGGAGACCTA GGCCTATTGA GGGCCTGGGC CTGGGCCTGG AGAAGTGTGA GGAGGAGCGC 
ATCATCAGCC GCGGGGGGTC CCTCAAGGGG GACGGAGGGG ACCTGTCGAG TGCCCTGGAC 
AACCAGAGGA GCCCTTTGTC CCTGGGCAAA CGGGAGCCAC CATGGCTGGC CAGGCCCTGT 
CATGGGAACT GTGATCCGAC CCAGCAGGAG GCTGGGGGAG GGGAGACCGT GGTGACCTTC 
GAGGACCGGG CCAGGCACAG GCAGAGCCAG CGGCGCAGCC GGCACCGTCG TGTCAGGACG 
GAAGGCAAGG AGCTCTCCTC CACCTCCCGG AGCAGGTCTG TCAGCCAGGA GCGGAGTCTG 
GATGAGGGGG TGCTCACCGA CGGGGAGAAG GAGCGAGAGC CCAGGGGCGG CCATGCAGGC 
AAAGAACCCA CCATCCAAGA AGAAGACAGA GGCCAAGACT TAAGGAGGAC GAACAGTCTG 
ATGGTGCCCA GAGGCTCTGG GCTGGCAGGA GCCCTTGATG AGGCCAACAC ACCCCTAGTC 
CTGCCCCACC ACGATCTGGA AGTGGGGAAG GACACGGTGC TGACGGAGCA GGAGCCGGAG 
GGCAGCAGCG AGCAGGCCCT GCTGGGGGAT GTGCAGTTGG ACATTGGCAG GGTCATCAGC 
CAGAGTGAGC CTGACCTCTC CTGCGTCACA GCCAACACAG ACAAGGCCAC CACCGACAGC 
ACCAGCGTCA CCGTCGCCAT CCCTGATGTG GGCCCCTTGA TGGACTCAAC TGTGATTCAC 
ATTAGCAACA AAACTGATGG AGAAGCCAGT CCCTTGAAGG AGGCTGAGAT CAAAGAGGAG 
GAGGAGGAAA TGGAGAAGAA GCAGCAGAAG AAGGAGAAGC GTGAGACGGG CAAAGCCATG 
GTGCCCCACA GCTCTATGTT CATCTTCAGC ACCACCAACC CGATTCGCAG GGCCTGCCAC 
TACATCGTGA ACCTGCGCTA CTTTGAGATG TGCATTCTCC TGGTGATCGC CGCCAGCAGC 
ATTGCCCTGG CAGCAGAGGA CCCCGTCCTG ACCAACTCGG AGCGCAACAA AGTCCTGAGG 
TATTTTGACT ATGTTTTCAC TGGCGTGTTC ACCTTTGAGA TGGTTATAAA GATGATAGAC 
CAGGGCTTGA TCCTACAGGA TGGGTCCTAC TTCCGAGACC TGTGGAACAT CCTGGACTTT 
GTGGTGGTGG TTGGTGCATT GGTGGCCTTT GCTTTGGCGA ACGCTTTGGG AACAAACAAG 
GGGCGAGACA TCAAGACCAT CAAATCCCTG AGGGTGCTCC GAGTTCTGAG GCCACTGAAG 
ACCATCAAGC GCTTGCCCAA ACTCAAGGCT GTCTTTGACT GTGTGGTGAC CTCCTTGAAG 
AATGTCTTCA ACATACTCAT CGTCTATAAG CTCTTCATGT TCATCTTTGC TGTCATTGCG 
GTTCAGCTCT TCAAGGGAAA ATTCTTTTAT TGCACAGACA GTTCCAAGGA CACAGAGAAG 
GAGTGCATAG GCAACTACGT AGATCATGAA AAAAACAAGA TGGAGGTGAA AGGCCGGGAA 
TGGAAGCGCC ATGAATTCCA CTACGACAAC ATTATCTGGG CCCTGCTGAC CCTCTTCACC 
GTCTCCACGG GGGAAGGATG GCCTCAAGTC TTACAGCATT CTGTCGATGT GACAGAGGAA 
GACCGAGGAC CAAGCCGCAG CAACCGCATG GAGATGTCCA TCTTCTATGT GGTCTACTTT 
GTGGTCTTCC CTTTCTTCTT TGTCAATATC TTTGTGGCTC TGATCATCAT CACCTTCCAG 
GAACAAGGGG ACAAGATGAT GGAGGAATGC AGCCTGGAGA AGAACGAGAG GGCGTGCATT 
GACTTCGCCA TCAGTGCCAA ACCTCTCACC CGCTACATGC CACAGAACAG GCACACCTTC 
CAGTACCGCG TCTGGCACTT CGTGGTGTCT CCGTCCTTTG AGTACACCAT CATGGCCATG 
ATCGCCCTGA ACACCGTCGT GCTGATGATG AAGTACTACT CTGCTCCCTG TACCTATGAA 
CTGGCCCTGA AGTACCTGAA TATTGCCTTC ACCATGGTGT TTTCCCTGGA ATGTGTCCTA 
AAGATCATCG CTTTTGGCTT CTTGAACTAC TTTCGAGACA CCTGGAACAT CTTTGACTTC 
ATCACTGTGA TTGGCAGTAT CACAGAAATC ATACTGACAG ACAGCAAGCT GGTAAACACC 
AGTGGTTTCA ACATGAGCTT TCTGAAGCTC TTCCGTGCCG CCCGCCTCAT CAAGCTTCTG 
CGGCAGGGCT ACACCATCCG TATTTTACTC TGGACCTTCG TGCAGTCCTT TAAGGCCCTC 
CCCTACGTCT GCCTTTTGAT TGCCATGCTT TTCTTCATCT ATGCCATCAT TGGGATGCAG 
GTATTTGGAA ACATAAAATT AGATGAGGAG AGTCACATCA ACCGGCACAA CAACTTCCGG 
AGTTTCTTTG GGTCCCTCAT GTTACTCTTC AGGAGTGCCA CGGGAGAGGC CTGGCAGGAG 
ATAATGCTGT CGTGCCTCGG GGAGAAAGGC TGCGAGCCCG ACACCACTGC GCCATCGGGG 
CAGAACGAGA ATGAGCGCTG TGGCACCGAT CTGGCCTATG TTTACTTTGT CTCCTTCATC 
TTCTTCTGCT CTTTCTTGAT GCTCAACTTG TTTGTGGCCG TCATCATGGA CAACTTCGAG 
TACCTGACTC GGGACTCCTC CATCTTAGGG CCCCACCACT TGGATGAGTT TGTCCGCGTC 
TGGGCAGAGT ACGACCGAGC TGCATGTGGC CGCATCCATT ATACTGAGAT GTATGAAATG 
CTGACTCTCA TGTCACCACC GCTAGGCCTC GGCAAGAGAT GTCCCTCCAA AGTGGCATAT 
AAGAGGTTGG TCCTAATGAA TATGCCAGTA GCTGAGGACA TGACAGTCCA CTTCACCTCC 
ACACTGATGG CTCTGATCCG GACAGCCCTG GACATCAAAA TCGCCAAAGG TGGTGCAGAC 
AGGCAGCAGC TAGACTCGGA GCTGCAAAAG GAGACCCTGG CCATCTGGCC TCACCTGTCC 
CAGAAGATGC TAGACCTGCT TGTGCCCATG CCCAAAGCCT CTGACCTGAC TGTGGGCAAA 
ATCTATGCAG CAATGATGAT CATGGACTAC TATAAACAGA GTAAGGTGAA GAAACAGAGG 
CAGCAGCTGG AGGAACAGAA AAATGCACCC ATGTTCCAGC GAATGGAGCC CTCATCTCTG 
CCTCAGGAGA TCATTGCTAA TGCCAAAGCC CTGCCCTATC TCCAGCAGGA CCCCGTTTCA 
GGCCTGAGTG GCCGTAGTGG ATACCCTTCG ATGAGTCCAC TCTCCCCCCA GGAAATATTC 
CAATTGGCTT GTATGGATCC AGCCGATGAT GGACAGTTCC AGGAACATCA GTCTCTGGTG 
GTGACAGATA CTAGCTCCAT GAGACGTTCA TTTTCCACTA TTCGGGATAA GCGTTCAAAT 
TCCTCCTGGT TGGAGGAATT CTCCATGGAG AGAAGCAGTG AAAACACCTA CAAGTCACGT 
CGTCGGAGTT ACCACTCCTC CCTGAGACTG TCAGCTCATC GCCTGAATTC TGACTCAGGC 
CACAAGTCTG ACACCCACCG CTCAGGGGGT AGAGAGCGGG GACGATCGAA AGAGCGAAAG 
CATCTTCTCT CTCCTGATGT CTCCCGCTGC AACTCAGAGG AGCGAGGGAC CCAGGCCGAC 
TGGGAGTCCC CAGAGCGCCA ACAGTCCAGG TCTCCCAGTG AGGCCAGGTC ACAGACCCCC 
AACAGACAGG GCACGGGCTC CCTGAGCGAG AGCTCCATCC CCTCCATCTC TGACACCAGC 
ACCCCAAGAC GAAGTCGCCG GCAGCTCCCA CCCGTCCCAC CAAAGCCTCG GCCCCTCCTC 
TCCTACAGCT CCCTGATTCG ACACTCCGGG AGCATCTCCC CGCCGGCCGA CGGAAGCGAG 
AGGGGCTCCC CGCTGACCCC CCAGGCGCTG GAGAGCACGG AGGCCGGCCT GGCGGAGCCC 
TCGGGCCCCC GGCACGGGCA CGGGCAGGGG CACGGGCAGG GGCACGGGCA GCAGAGCCAG 
CACTGCTCCC CGCAGCGCTA CATCTCCGAG CCCTACCTGG CCCTGCACGA AGACTCCCAC 
GCGTCGGACT GTGGCGAGGA GGAGACGCTC ACCTTCGAGG CCGCCGTGGC CACCAGCCTG 
GGCCGCTCCA ACACCATCGG CTCGGCCCCG CCGCTGCGGC ACAGCTGGCA GATGCCCAAC 
GGGCACTATC GGCGGCGGCG GCGCGGGGGG CCGGGGCCGG GCGTGATGTG CGGGGCGGTC 
AGCGACCTCC TGAGTGACAC GGAAGAAGAC GACAAGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_003434969.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003435017.2 Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OCe09120
Clone ID Related Accession (Same CDS sequence)	XM_003434969.3
Accession Version	XM_003434969.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003434969.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGC AATGTTCTGG GATGGTGCCC GAGATGCGTG GACCGAGCCC AGGCCGCAGG 
CTGCCCGAGC CCGCCCCCTG CCCCGCCGCG GCCGGGAGCC CTGAGCCCTG GGCCGCGGGC 
CGCCGCCGCC ACGTCCTGGC CCTGCCTGAG GAGGAGGCAT GGAGGCCCCA GCAGTGCTCC 
CTGCTCGAGG CCGACGCCTC GGACGAGGTG GGCATGCTGC CGGCCTCCCC GCGCGCCGCC 
GCCTCCGGCG TCGACAACTT CTTCCCGGTG CAGGAGGGCG AGGGCGCGGG CTGGGAGGGC 
GGTGCGGCCC TGGACGCCGG CGCCGGCCCC TTCCTCCCGG TGAGCCCCGA GGTGATGAAG 
CGGCGGCGCG GGGGCCTCAT CGAGCAGCGC GACATCATCA AGGCCCACGA GGCGCACAAG 
ATGCAGAGCA CCCCGCAGGC GCGGCGCAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG 
GTGGTCGGCA GGCCGGGGTC GGGCGATGGA GACTCGGACC AGAGCAGGAA CCGGCAAGGA 
ACCCCGGTGC CGGCCTCGGG GCCGGCGGCC GCCTACAAGC AGTCGAAAGC GCAGAGGGCG 
CGGACTATGG CTTTGTACAA CCCCATTCCC GTCCGGCAGA ACTGTTTCAC CGTCAACAGA 
TCCCTGTTCA TCTTCGGAGA AGATAACATT GTCAGGAAAT ACGCCAAGAA GCTCATCGAC 
TGGCCGCCGT TTGAGTACAT GATTTTGGCA ACCATCATTG CCAACTGCAT TGTCTTGGCC 
CTGGAACAGC ATCTTCCTGA GGATGACAAG ACCCCGATGT CCCGCAGACT GGAGAAGACA 
GAGCCGTATT TCATTGGGAT CTTTTGCTTT GAAGCCGGTA TCAAAATTGT GGCCCTGGGG 
TTCATCTTTC ATAAGGGCTC GTACCTCCGC AATGGCTGGA ATGTCATGGA CTTCATCGTG 
GTCCTCAGTG GCATCCTGGC CACTGCGGGG ACTCACTTCA ACACACACGT GGACCTGAGG 
ACCCTCCGGG CTGTGCGTGT CCTGAGACCC CTGAAGCTCG TGTCAGGGAT TCCTAGCCTG 
CAGATCGTGT TGAAGTCTAT CATGAAGGCC ATGGTGCCTC TTCTGCAAAT TGGTCTCCTG 
CTCTTCTTTG CCATCTTGAT GTTTGCTATC ATTGGCTTAG AGTTCTACAG TGGGAAGTTG 
CACCGAGCAT GTTTCACGAA CAATTCAGGT ATTCTGGAAG GATTTGACCC CCCTCACCCA 
TGTGGTGTGC AGGGCTGCCC AGCTGGCTAC GAATGCCGGG ACTGGATTGG CCCCAATGAT 
GGGATCACCC AGTTTGATAA CATCCTTTTT GCTGTGCTGA CTGTCTTCCA ATGCATCACC 
ATGGAAGGAT GGACCACCGT GCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC 
TGGCTGTACT TCATCCCCCT CATCATCATT GGATCCTTCT TTGTTCTCAA CCTAGTTCTG 
GGAGTGCTTT CCGGGGAGTT TGCCAAAGAG AGAGAGAGAG TGGAGAACCG GAGAGCTTTC 
ATGAAACTGC GGCGGCAGCA GCAGATTGAG CGGGAGCTGA ACGGGTACCG CGCCTGGATA 
GACAAAGCAG AGGAAGTCAT GCTTGCTGAA GAAAATAAAA ATGCTGGGAC GTCAGCCTTA 
GAAGTGCTTC GAAGGGCAAC CATCAAAAGG AGCCGGACAG AGGCCATGAC TCGAGACTCC 
AGTGATGAAC ACTGTGTCGA TATCTCCTCT GTGGGCACAC CTCTCGCCCG AGCCAGCATC 
AAAAGTGCAA AGGTGGATGG GGCCTCCTAT TTCCGGCACA AAGAAAGGCT TCTGCGCATC 
TCTATCCGCC ACATGGTGAA ATCCCAGGTG TTTTACTGGA TTGTGTTGAG CCTTGTGGCT 
CTCAACACTG CCTGTGTGGC CATCGTCCAT CATAACCAGC CCCAGTGGCT CACCCATCTC 
CTCTACTATG CAGAATTTTT GTTTCTGGGA CTGTTCCTCT TGGAGATGTC CCTGAAGATG 
TATGGCATGG GACCTCGACT TTATTTTCAC TCTTCATTCA ACTGTTTTGA TTTTGGGGTC 
ACAGTGGGCA GCATCTTTGA AGTGGTCTGG GCAATCTTCA GACCTGGCAC ATCTTTTGGA 
ATCAGTGTCT TACGGGCTCT CCGGCTTCTA AGAATATTTA AAATAACCAA GTATTGGGCA 
TCTCTGCGAA ATTTGGTGGT CTCCTTGATG AGCTCTATGA AGTCTATCAT CAGTTTGCTC 
TTCCTCCTCT TCCTCTTCAT CGTTGTCTTT GCTCTCCTAG GAATGCAGCT GTTTGGAGGC 
AGGTTTAACT TTAATGATGG GACTCCTTCA GCAAACTTTG ATACCTTCCC TGCAGCCATC 
ATGACTGTAT TCCAGATCCT AACAGGTGAG GACTGGAATG AGGTGATGTA CAATGGGATC 
CGTTCCCAGG GTGGAGTTAG CTCAGGCATG TGGTCAGCTA TCTATTTCAT TGTGCTCACC 
TTGTTTGGAA ACTACACACT GCTGAATGTG TTCTTGGCCA TTGCTGTGGA CAATCTGGCC 
AATGCCCAAG AGCTGACCAA GGATGAACAA GAAGAAGAGG AGGCTTTTAA CCAGAAACAT 
GCATTGCAGA AGGCCAAGGA GGTCAGCCCG ATGTCTGCAC CCAACATGCC TTCTATCGAG 
AGGGAGCGGA GGCGCCGGCA CCACATGTCG GTGTGGGAGC AGCGCACCAG CCAGCTGAGA 
AGGCACATGC AGATGTCCAG CCAGGAGGCC CTCAATAAAG AGGAGGCCCC ACCGATGAAC 
CCCCTCAACC CCCTCAACCC GCTGAGCCCC CTCAACCCGC TCAACGCCCA CCCCAGCCTC 
TACCGGAGAC CTAGGCCTAT TGAGGGCCTG GGCCTGGGCC TGGAGAAGTG TGAGGAGGAG 
CGCATCATCA GCCGCGGGGG GTCCCTCAAG GGGGACGGAG GGGACCTGTC GAGTGCCCTG 
GACAACCAGA GGAGCCCTTT GTCCCTGGGC AAACGGGAGC CACCATGGCT GGCCAGGCCC 
TGTCATGGGA ACTGTGATCC GACCCAGCAG GAGGCTGGGG GAGGGGAGAC CGTGGTGACC 
TTCGAGGACC GGGCCAGGCA CAGGCAGAGC CAGCGGCGCA GCCGGCACCG TCGTGTCAGG 
ACGGAAGGCA AGGAGCTCTC CTCCACCTCC CGGAGCAGGT CTGTCAGCCA GGAGCGGAGT 
CTGGATGAGG GGGTGCTCAC CGACGGGGAG AAGGAGCGAG AGCCCAGGGG CGGCCATGCA 
GGCAAAGAAC CCACCATCCA AGAAGAAGAC AGAGGCCAAG ACTTAAGGAG GACGAACAGT 
CTGATGGTGC CCAGAGGCTC TGGGCTGGCA GGAGCCCTTG ATGAGGCCAA CACACCCCTA 
GTCCTGCCCC ACCACGATCT GGAAGTGGGG AAGGACACGG TGCTGACGGA GCAGGAGCCG 
GAGGGCAGCA GCGAGCAGGC CCTGCTGGGG GATGTGCAGT TGGACATTGG CAGGGTCATC 
AGCCAGAGTG AGCCTGACCT CTCCTGCGTC ACAGCCAACA CAGACAAGGC CACCACCGAC 
AGCACCAGCG TCACCGTCGC CATCCCTGAT GTGGGCCCCT TGATGGACTC AACTGTGATT 
CACATTAGCA ACAAAACTGA TGGAGAAGCC AGTCCCTTGA AGGAGGCTGA GATCAAAGAG 
GAGGAGGAGG AAATGGAGAA GAAGCAGCAG AAGAAGGAGA AGCGTGAGAC GGGCAAAGCC 
ATGGTGCCCC ACAGCTCTAT GTTCATCTTC AGCACCACCA ACCCGATTCG CAGGGCCTGC 
CACTACATCG TGAACCTGCG CTACTTTGAG ATGTGCATTC TCCTGGTGAT CGCCGCCAGC 
AGCATTGCCC TGGCAGCAGA GGACCCCGTC CTGACCAACT CGGAGCGCAA CAAAGTCCTG 
AGGTATTTTG ACTATGTTTT CACTGGCGTG TTCACCTTTG AGATGGTTAT AAAGATGATA 
GACCAGGGCT TGATCCTACA GGATGGGTCC TACTTCCGAG ACCTGTGGAA CATCCTGGAC 
TTTGTGGTGG TGGTTGGTGC ATTGGTGGCC TTTGCTTTGG CGAACGCTTT GGGAACAAAC 
AAGGGGCGAG ACATCAAGAC CATCAAATCC CTGAGGGTGC TCCGAGTTCT GAGGCCACTG 
AAGACCATCA AGCGCTTGCC CAAACTCAAG GCTGTCTTTG ACTGTGTGGT GACCTCCTTG 
AAGAATGTCT TCAACATACT CATCGTCTAT AAGCTCTTCA TGTTCATCTT TGCTGTCATT 
GCGGTTCAGC TCTTCAAGGG AAAATTCTTT TATTGCACAG ACAGTTCCAA GGACACAGAG 
AAGGAGTGCA TAGGCAACTA CGTAGATCAT GAAAAAAACA AGATGGAGGT GAAAGGCCGG 
GAATGGAAGC GCCATGAATT CCACTACGAC AACATTATCT GGGCCCTGCT GACCCTCTTC 
ACCGTCTCCA CGGGGGAAGG ATGGCCTCAA GTCTTACAGC ATTCTGTCGA TGTGACAGAG 
GAAGACCGAG GACCAAGCCG CAGCAACCGC ATGGAGATGT CCATCTTCTA TGTGGTCTAC 
TTTGTGGTCT TCCCTTTCTT CTTTGTCAAT ATCTTTGTGG CTCTGATCAT CATCACCTTC 
CAGGAACAAG GGGACAAGAT GATGGAGGAA TGCAGCCTGG AGAAGAACGA GAGGGCGTGC 
ATTGACTTCG CCATCAGTGC CAAACCTCTC ACCCGCTACA TGCCACAGAA CAGGCACACC 
TTCCAGTACC GCGTCTGGCA CTTCGTGGTG TCTCCGTCCT TTGAGTACAC CATCATGGCC 
ATGATCGCCC TGAACACCGT CGTGCTGATG ATGAAGTACT ACTCTGCTCC CTGTACCTAT 
GAACTGGCCC TGAAGTACCT GAATATTGCC TTCACCATGG TGTTTTCCCT GGAATGTGTC 
CTAAAGATCA TCGCTTTTGG CTTCTTGAAC TACTTTCGAG ACACCTGGAA CATCTTTGAC 
TTCATCACTG TGATTGGCAG TATCACAGAA ATCATACTGA CAGACAGCAA GCTGGTAAAC 
ACCAGTGGTT TCAACATGAG CTTTCTGAAG CTCTTCCGTG CCGCCCGCCT CATCAAGCTT 
CTGCGGCAGG GCTACACCAT CCGTATTTTA CTCTGGACCT TCGTGCAGTC CTTTAAGGCC 
CTCCCCTACG TCTGCCTTTT GATTGCCATG CTTTTCTTCA TCTATGCCAT CATTGGGATG 
CAGGTATTTG GAAACATAAA ATTAGATGAG GAGAGTCACA TCAACCGGCA CAACAACTTC 
CGGAGTTTCT TTGGGTCCCT CATGTTACTC TTCAGGAGTG CCACGGGAGA GGCCTGGCAG 
GAGATAATGC TGTCGTGCCT CGGGGAGAAA GGCTGCGAGC CCGACACCAC TGCGCCATCG 
GGGCAGAACG AGAATGAGCG CTGTGGCACC GATCTGGCCT ATGTTTACTT TGTCTCCTTC 
ATCTTCTTCT GCTCTTTCTT GATGCTCAAC TTGTTTGTGG CCGTCATCAT GGACAACTTC 
GAGTACCTGA CTCGGGACTC CTCCATCTTA GGGCCCCACC ACTTGGATGA GTTTGTCCGC 
GTCTGGGCAG AGTACGACCG AGCTGCATGT GGCCGCATCC ATTATACTGA GATGTATGAA 
ATGCTGACTC TCATGTCACC ACCGCTAGGC CTCGGCAAGA GATGTCCCTC CAAAGTGGCA 
TATAAGAGGT TGGTCCTAAT GAATATGCCA GTAGCTGAGG ACATGACAGT CCACTTCACC 
TCCACACTGA TGGCTCTGAT CCGGACAGCC CTGGACATCA AAATCGCCAA AGGTGGTGCA 
GACAGGCAGC AGCTAGACTC GGAGCTGCAA AAGGAGACCC TGGCCATCTG GCCTCACCTG 
TCCCAGAAGA TGCTAGACCT GCTTGTGCCC ATGCCCAAAG CCTCTGACCT GACTGTGGGC 
AAAATCTATG CAGCAATGAT GATCATGGAC TACTATAAAC AGAGTAAGGT GAAGAAACAG 
AGGCAGCAGC TGGAGGAACA GAAAAATGCA CCCATGTTCC AGCGAATGGA GCCCTCATCT 
CTGCCTCAGG AGATCATTGC TAATGCCAAA GCCCTGCCCT ATCTCCAGCA GGACCCCGTT 
TCAGGCCTGA GTGGCCGTAG TGGATACCCT TCGATGAGTC CACTCTCCCC CCAGGAAATA 
TTCCAATTGG CTTGTATGGA TCCAGCCGAT GATGGACAGT TCCAGGAACA TCAGTCTCTG 
GTGGTGACAG ATACTAGCTC CATGAGACGT TCATTTTCCA CTATTCGGGA TAAGCGTTCA 
AATTCCTCCT GGTTGGAGGA ATTCTCCATG GAGAGAAGCA GTGAAAACAC CTACAAGTCA 
CGTCGTCGGA GTTACCACTC CTCCCTGAGA CTGTCAGCTC ATCGCCTGAA TTCTGACTCA 
GGCCACAAGT CTGACACCCA CCGCTCAGGG GGTAGAGAGC GGGGACGATC GAAAGAGCGA 
AAGCATCTTC TCTCTCCTGA TGTCTCCCGC TGCAACTCAG AGGAGCGAGG GACCCAGGCC 
GACTGGGAGT CCCCAGAGCG CCAACAGTCC AGGTCTCCCA GTGAGGCCAG GTCACAGACC 
CCCAACAGAC AGGGCACGGG CTCCCTGAGC GAGAGCTCCA TCCCCTCCAT CTCTGACACC 
AGCACCCCAA GACGAAGTCG CCGGCAGCTC CCACCCGTCC CACCAAAGCC TCGGCCCCTC 
CTCTCCTACA GCTCCCTGAT TCGACACTCC GGGAGCATCT CCCCGCCGGC CGACGGAAGC 
GAGAGGGGCT CCCCGCTGAC CCCCCAGGCG CTGGAGAGCA CGGAGGCCGG CCTGGCGGAG 
CCCTCGGGCC CCCGGCACGG GCACGGGCAG GGGCACGGGC AGGGGCACGG GCAGCAGAGC 
CAGCACTGCT CCCCGCAGCG CTACATCTCC GAGCCCTACC TGGCCCTGCA CGAAGACTCC 
CACGCGTCGG ACTGTGGCGA GGAGGAGACG CTCACCTTCG AGGCCGCCGT GGCCACCAGC 
CTGGGCCGCT CCAACACCAT CGGCTCGGCC CCGCCGCTGC GGCACAGCTG GCAGATGCCC 
AACGGGCACT ATCGGCGGCG GCGGCGCGGG GGGCCGGGGC CGGGCGTGAT GTGCGGGGCG 
GTCAGCGACC TCCTGAGTGA CACGGAAGAA GACGACAAGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003435017.2
CDS	85..7329
Translation	MEEQCSGMVPEMRGPSPGRRLPEPAPCPAAAGSPEPWAAGRRRHVLALPEEEAWRPQQCSLLEADASDEVGMLPASPRAAASGVDNFFPVQEGEGAGWEGGAALDAGAGPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFTNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLGLGLEKCEEERIISRGGSLKGDGGDLSSALDNQRSPLSLGKREPPWLARPCHGNCDPTQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKELSSTSRSRSVSQERSLDEGVLTDGEKEREPRGGHAGKEPTIQEEDRGQDLRRTNSLMVPRGSGLAGALDEANTPLVLPHHDLEVGKDTVLTEQEPEGSSEQALLGDVQLDIGRVISQSEPDLSCVTANTDKATTDSTSVTVAIPDVGPLMDSTVIHISNKTDGEASPLKEAEIKEEEEEMEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEHQSLVVTDTSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERQQSRSPSEARSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHSGSISPPADGSERGSPLTPQALESTEAGLAEPSGPRHGHGQGHGQGHGQQSQHCSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGVMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_003434969.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003434969.3

ORF Insert Sequence:

ATGGAAGAGC AATGTTCTGG GATGGTGCCC GAGATGCGTG GACCGAGCCC AGGCCGCAGG 
CTGCCCGAGC CCGCCCCCTG CCCCGCCGCG GCCGGGAGCC CTGAGCCCTG GGCCGCGGGC 
CGCCGCCGCC ACGTCCTGGC CCTGCCTGAG GAGGAGGCAT GGAGGCCCCA GCAGTGCTCC 
CTGCTCGAGG CCGACGCCTC GGACGAGGTG GGCATGCTGC CGGCCTCCCC GCGCGCCGCC 
GCCTCCGGCG TCGACAACTT CTTCCCGGTG CAGGAGGGCG AGGGCGCGGG CTGGGAGGGC 
GGTGCGGCCC TGGACGCCGG CGCCGGCCCC TTCCTCCCGG TGAGCCCCGA GGTGATGAAG 
CGGCGGCGCG GGGGCCTCAT CGAGCAGCGC GACATCATCA AGGCCCACGA GGCGCACAAG 
ATGCAGAGCA CCCCGCAGGC GCGGCGCAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG 
GTGGTCGGCA GGCCGGGGTC GGGCGATGGA GACTCGGACC AGAGCAGGAA CCGGCAAGGA 
ACCCCGGTGC CGGCCTCGGG GCCGGCGGCC GCCTACAAGC AGTCGAAAGC GCAGAGGGCG 
CGGACTATGG CTTTGTACAA CCCCATTCCC GTCCGGCAGA ACTGTTTCAC CGTCAACAGA 
TCCCTGTTCA TCTTCGGAGA AGATAACATT GTCAGGAAAT ACGCCAAGAA GCTCATCGAC 
TGGCCGCCGT TTGAGTACAT GATTTTGGCA ACCATCATTG CCAACTGCAT TGTCTTGGCC 
CTGGAACAGC ATCTTCCTGA GGATGACAAG ACCCCGATGT CCCGCAGACT GGAGAAGACA 
GAGCCGTATT TCATTGGGAT CTTTTGCTTT GAAGCCGGTA TCAAAATTGT GGCCCTGGGG 
TTCATCTTTC ATAAGGGCTC GTACCTCCGC AATGGCTGGA ATGTCATGGA CTTCATCGTG 
GTCCTCAGTG GCATCCTGGC CACTGCGGGG ACTCACTTCA ACACACACGT GGACCTGAGG 
ACCCTCCGGG CTGTGCGTGT CCTGAGACCC CTGAAGCTCG TGTCAGGGAT TCCTAGCCTG 
CAGATCGTGT TGAAGTCTAT CATGAAGGCC ATGGTGCCTC TTCTGCAAAT TGGTCTCCTG 
CTCTTCTTTG CCATCTTGAT GTTTGCTATC ATTGGCTTAG AGTTCTACAG TGGGAAGTTG 
CACCGAGCAT GTTTCACGAA CAATTCAGGT ATTCTGGAAG GATTTGACCC CCCTCACCCA 
TGTGGTGTGC AGGGCTGCCC AGCTGGCTAC GAATGCCGGG ACTGGATTGG CCCCAATGAT 
GGGATCACCC AGTTTGATAA CATCCTTTTT GCTGTGCTGA CTGTCTTCCA ATGCATCACC 
ATGGAAGGAT GGACCACCGT GCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC 
TGGCTGTACT TCATCCCCCT CATCATCATT GGATCCTTCT TTGTTCTCAA CCTAGTTCTG 
GGAGTGCTTT CCGGGGAGTT TGCCAAAGAG AGAGAGAGAG TGGAGAACCG GAGAGCTTTC 
ATGAAACTGC GGCGGCAGCA GCAGATTGAG CGGGAGCTGA ACGGGTACCG CGCCTGGATA 
GACAAAGCAG AGGAAGTCAT GCTTGCTGAA GAAAATAAAA ATGCTGGGAC GTCAGCCTTA 
GAAGTGCTTC GAAGGGCAAC CATCAAAAGG AGCCGGACAG AGGCCATGAC TCGAGACTCC 
AGTGATGAAC ACTGTGTCGA TATCTCCTCT GTGGGCACAC CTCTCGCCCG AGCCAGCATC 
AAAAGTGCAA AGGTGGATGG GGCCTCCTAT TTCCGGCACA AAGAAAGGCT TCTGCGCATC 
TCTATCCGCC ACATGGTGAA ATCCCAGGTG TTTTACTGGA TTGTGTTGAG CCTTGTGGCT 
CTCAACACTG CCTGTGTGGC CATCGTCCAT CATAACCAGC CCCAGTGGCT CACCCATCTC 
CTCTACTATG CAGAATTTTT GTTTCTGGGA CTGTTCCTCT TGGAGATGTC CCTGAAGATG 
TATGGCATGG GACCTCGACT TTATTTTCAC TCTTCATTCA ACTGTTTTGA TTTTGGGGTC 
ACAGTGGGCA GCATCTTTGA AGTGGTCTGG GCAATCTTCA GACCTGGCAC ATCTTTTGGA 
ATCAGTGTCT TACGGGCTCT CCGGCTTCTA AGAATATTTA AAATAACCAA GTATTGGGCA 
TCTCTGCGAA ATTTGGTGGT CTCCTTGATG AGCTCTATGA AGTCTATCAT CAGTTTGCTC 
TTCCTCCTCT TCCTCTTCAT CGTTGTCTTT GCTCTCCTAG GAATGCAGCT GTTTGGAGGC 
AGGTTTAACT TTAATGATGG GACTCCTTCA GCAAACTTTG ATACCTTCCC TGCAGCCATC 
ATGACTGTAT TCCAGATCCT AACAGGTGAG GACTGGAATG AGGTGATGTA CAATGGGATC 
CGTTCCCAGG GTGGAGTTAG CTCAGGCATG TGGTCAGCTA TCTATTTCAT TGTGCTCACC 
TTGTTTGGAA ACTACACACT GCTGAATGTG TTCTTGGCCA TTGCTGTGGA CAATCTGGCC 
AATGCCCAAG AGCTGACCAA GGATGAACAA GAAGAAGAGG AGGCTTTTAA CCAGAAACAT 
GCATTGCAGA AGGCCAAGGA GGTCAGCCCG ATGTCTGCAC CCAACATGCC TTCTATCGAG 
AGGGAGCGGA GGCGCCGGCA CCACATGTCG GTGTGGGAGC AGCGCACCAG CCAGCTGAGA 
AGGCACATGC AGATGTCCAG CCAGGAGGCC CTCAATAAAG AGGAGGCCCC ACCGATGAAC 
CCCCTCAACC CCCTCAACCC GCTGAGCCCC CTCAACCCGC TCAACGCCCA CCCCAGCCTC 
TACCGGAGAC CTAGGCCTAT TGAGGGCCTG GGCCTGGGCC TGGAGAAGTG TGAGGAGGAG 
CGCATCATCA GCCGCGGGGG GTCCCTCAAG GGGGACGGAG GGGACCTGTC GAGTGCCCTG 
GACAACCAGA GGAGCCCTTT GTCCCTGGGC AAACGGGAGC CACCATGGCT GGCCAGGCCC 
TGTCATGGGA ACTGTGATCC GACCCAGCAG GAGGCTGGGG GAGGGGAGAC CGTGGTGACC 
TTCGAGGACC GGGCCAGGCA CAGGCAGAGC CAGCGGCGCA GCCGGCACCG TCGTGTCAGG 
ACGGAAGGCA AGGAGCTCTC CTCCACCTCC CGGAGCAGGT CTGTCAGCCA GGAGCGGAGT 
CTGGATGAGG GGGTGCTCAC CGACGGGGAG AAGGAGCGAG AGCCCAGGGG CGGCCATGCA 
GGCAAAGAAC CCACCATCCA AGAAGAAGAC AGAGGCCAAG ACTTAAGGAG GACGAACAGT 
CTGATGGTGC CCAGAGGCTC TGGGCTGGCA GGAGCCCTTG ATGAGGCCAA CACACCCCTA 
GTCCTGCCCC ACCACGATCT GGAAGTGGGG AAGGACACGG TGCTGACGGA GCAGGAGCCG 
GAGGGCAGCA GCGAGCAGGC CCTGCTGGGG GATGTGCAGT TGGACATTGG CAGGGTCATC 
AGCCAGAGTG AGCCTGACCT CTCCTGCGTC ACAGCCAACA CAGACAAGGC CACCACCGAC 
AGCACCAGCG TCACCGTCGC CATCCCTGAT GTGGGCCCCT TGATGGACTC AACTGTGATT 
CACATTAGCA ACAAAACTGA TGGAGAAGCC AGTCCCTTGA AGGAGGCTGA GATCAAAGAG 
GAGGAGGAGG AAATGGAGAA GAAGCAGCAG AAGAAGGAGA AGCGTGAGAC GGGCAAAGCC 
ATGGTGCCCC ACAGCTCTAT GTTCATCTTC AGCACCACCA ACCCGATTCG CAGGGCCTGC 
CACTACATCG TGAACCTGCG CTACTTTGAG ATGTGCATTC TCCTGGTGAT CGCCGCCAGC 
AGCATTGCCC TGGCAGCAGA GGACCCCGTC CTGACCAACT CGGAGCGCAA CAAAGTCCTG 
AGGTATTTTG ACTATGTTTT CACTGGCGTG TTCACCTTTG AGATGGTTAT AAAGATGATA 
GACCAGGGCT TGATCCTACA GGATGGGTCC TACTTCCGAG ACCTGTGGAA CATCCTGGAC 
TTTGTGGTGG TGGTTGGTGC ATTGGTGGCC TTTGCTTTGG CGAACGCTTT GGGAACAAAC 
AAGGGGCGAG ACATCAAGAC CATCAAATCC CTGAGGGTGC TCCGAGTTCT GAGGCCACTG 
AAGACCATCA AGCGCTTGCC CAAACTCAAG GCTGTCTTTG ACTGTGTGGT GACCTCCTTG 
AAGAATGTCT TCAACATACT CATCGTCTAT AAGCTCTTCA TGTTCATCTT TGCTGTCATT 
GCGGTTCAGC TCTTCAAGGG AAAATTCTTT TATTGCACAG ACAGTTCCAA GGACACAGAG 
AAGGAGTGCA TAGGCAACTA CGTAGATCAT GAAAAAAACA AGATGGAGGT GAAAGGCCGG 
GAATGGAAGC GCCATGAATT CCACTACGAC AACATTATCT GGGCCCTGCT GACCCTCTTC 
ACCGTCTCCA CGGGGGAAGG ATGGCCTCAA GTCTTACAGC ATTCTGTCGA TGTGACAGAG 
GAAGACCGAG GACCAAGCCG CAGCAACCGC ATGGAGATGT CCATCTTCTA TGTGGTCTAC 
TTTGTGGTCT TCCCTTTCTT CTTTGTCAAT ATCTTTGTGG CTCTGATCAT CATCACCTTC 
CAGGAACAAG GGGACAAGAT GATGGAGGAA TGCAGCCTGG AGAAGAACGA GAGGGCGTGC 
ATTGACTTCG CCATCAGTGC CAAACCTCTC ACCCGCTACA TGCCACAGAA CAGGCACACC 
TTCCAGTACC GCGTCTGGCA CTTCGTGGTG TCTCCGTCCT TTGAGTACAC CATCATGGCC 
ATGATCGCCC TGAACACCGT CGTGCTGATG ATGAAGTACT ACTCTGCTCC CTGTACCTAT 
GAACTGGCCC TGAAGTACCT GAATATTGCC TTCACCATGG TGTTTTCCCT GGAATGTGTC 
CTAAAGATCA TCGCTTTTGG CTTCTTGAAC TACTTTCGAG ACACCTGGAA CATCTTTGAC 
TTCATCACTG TGATTGGCAG TATCACAGAA ATCATACTGA CAGACAGCAA GCTGGTAAAC 
ACCAGTGGTT TCAACATGAG CTTTCTGAAG CTCTTCCGTG CCGCCCGCCT CATCAAGCTT 
CTGCGGCAGG GCTACACCAT CCGTATTTTA CTCTGGACCT TCGTGCAGTC CTTTAAGGCC 
CTCCCCTACG TCTGCCTTTT GATTGCCATG CTTTTCTTCA TCTATGCCAT CATTGGGATG 
CAGGTATTTG GAAACATAAA ATTAGATGAG GAGAGTCACA TCAACCGGCA CAACAACTTC 
CGGAGTTTCT TTGGGTCCCT CATGTTACTC TTCAGGAGTG CCACGGGAGA GGCCTGGCAG 
GAGATAATGC TGTCGTGCCT CGGGGAGAAA GGCTGCGAGC CCGACACCAC TGCGCCATCG 
GGGCAGAACG AGAATGAGCG CTGTGGCACC GATCTGGCCT ATGTTTACTT TGTCTCCTTC 
ATCTTCTTCT GCTCTTTCTT GATGCTCAAC TTGTTTGTGG CCGTCATCAT GGACAACTTC 
GAGTACCTGA CTCGGGACTC CTCCATCTTA GGGCCCCACC ACTTGGATGA GTTTGTCCGC 
GTCTGGGCAG AGTACGACCG AGCTGCATGT GGCCGCATCC ATTATACTGA GATGTATGAA 
ATGCTGACTC TCATGTCACC ACCGCTAGGC CTCGGCAAGA GATGTCCCTC CAAAGTGGCA 
TATAAGAGGT TGGTCCTAAT GAATATGCCA GTAGCTGAGG ACATGACAGT CCACTTCACC 
TCCACACTGA TGGCTCTGAT CCGGACAGCC CTGGACATCA AAATCGCCAA AGGTGGTGCA 
GACAGGCAGC AGCTAGACTC GGAGCTGCAA AAGGAGACCC TGGCCATCTG GCCTCACCTG 
TCCCAGAAGA TGCTAGACCT GCTTGTGCCC ATGCCCAAAG CCTCTGACCT GACTGTGGGC 
AAAATCTATG CAGCAATGAT GATCATGGAC TACTATAAAC AGAGTAAGGT GAAGAAACAG 
AGGCAGCAGC TGGAGGAACA GAAAAATGCA CCCATGTTCC AGCGAATGGA GCCCTCATCT 
CTGCCTCAGG AGATCATTGC TAATGCCAAA GCCCTGCCCT ATCTCCAGCA GGACCCCGTT 
TCAGGCCTGA GTGGCCGTAG TGGATACCCT TCGATGAGTC CACTCTCCCC CCAGGAAATA 
TTCCAATTGG CTTGTATGGA TCCAGCCGAT GATGGACAGT TCCAGGAACA TCAGTCTCTG 
GTGGTGACAG ATACTAGCTC CATGAGACGT TCATTTTCCA CTATTCGGGA TAAGCGTTCA 
AATTCCTCCT GGTTGGAGGA ATTCTCCATG GAGAGAAGCA GTGAAAACAC CTACAAGTCA 
CGTCGTCGGA GTTACCACTC CTCCCTGAGA CTGTCAGCTC ATCGCCTGAA TTCTGACTCA 
GGCCACAAGT CTGACACCCA CCGCTCAGGG GGTAGAGAGC GGGGACGATC GAAAGAGCGA 
AAGCATCTTC TCTCTCCTGA TGTCTCCCGC TGCAACTCAG AGGAGCGAGG GACCCAGGCC 
GACTGGGAGT CCCCAGAGCG CCAACAGTCC AGGTCTCCCA GTGAGGCCAG GTCACAGACC 
CCCAACAGAC AGGGCACGGG CTCCCTGAGC GAGAGCTCCA TCCCCTCCAT CTCTGACACC 
AGCACCCCAA GACGAAGTCG CCGGCAGCTC CCACCCGTCC CACCAAAGCC TCGGCCCCTC 
CTCTCCTACA GCTCCCTGAT TCGACACTCC GGGAGCATCT CCCCGCCGGC CGACGGAAGC 
GAGAGGGGCT CCCCGCTGAC CCCCCAGGCG CTGGAGAGCA CGGAGGCCGG CCTGGCGGAG 
CCCTCGGGCC CCCGGCACGG GCACGGGCAG GGGCACGGGC AGGGGCACGG GCAGCAGAGC 
CAGCACTGCT CCCCGCAGCG CTACATCTCC GAGCCCTACC TGGCCCTGCA CGAAGACTCC 
CACGCGTCGG ACTGTGGCGA GGAGGAGACG CTCACCTTCG AGGCCGCCGT GGCCACCAGC 
CTGGGCCGCT CCAACACCAT CGGCTCGGCC CCGCCGCTGC GGCACAGCTG GCAGATGCCC 
AACGGGCACT ATCGGCGGCG GCGGCGCGGG GGGCCGGGGC CGGGCGTGAT GTGCGGGGCG 
GTCAGCGACC TCCTGAGTGA CACGGAAGAA GACGACAAGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_005622396.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622453.1 Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OCe09121
Clone ID Related Accession (Same CDS sequence)	XM_005622396.1
Accession Version	XM_005622396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCGGTG CCGGCCTCGG GGCCGGCGGC CGCCTACAAG 
CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TACGCCAAGA AGCTCATCGA CTGGCCGCCG TTTGAGTACA TGATTTTGGC AACCATCATT 
GCCAACTGCA TTGTCTTGGC CCTGGAACAG CATCTTCCTG AGGATGACAA GACCCCGATG 
TCCCGCAGAC TGGAGAAGAC AGAGCCGTAT TTCATTGGGA TCTTTTGCTT TGAAGCCGGT 
ATCAAAATTG TGGCCCTGGG GTTCATCTTT CATAAGGGCT CGTACCTCCG CAATGGCTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCGGG GACTCACTTC 
AACACACACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGAGACC CCTGAAGCTC 
GTGTCAGGGA TTCCTAGCCT GCAGATCGTG TTGAAGTCTA TCATGAAGGC CATGGTGCCT 
CTTCTGCAAA TTGGTCTCCT GCTCTTCTTT GCCATCTTGA TGTTTGCTAT CATTGGCTTA 
GAGTTCTACA GTGGGAAGTT GCACCGAGCA TGTTTCACGA ACAATTCAGG TATTCTGGAA 
GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGCTA CGAATGCCGG 
GACTGGATTG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG 
ACTGTCTTCC AATGCATCAC CATGGAAGGA TGGACCACCG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA CTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTAGTTCT GGGAGTGCTT TCCGGGGAGT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GGAGAGCTTT CATGAAACTG CGGCGGCAGC AGCAGATTGA GCGGGAGCTG 
AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAATAAA 
AATGCTGGGA CGTCAGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAAAG GAGCCGGACA 
GAGGCCATGA CTCGAGACTC CAGTGATGAA CACTGTGTCG ATATCTCCTC TGTGGGCACA 
CCTCTCGCCC GAGCCAGCAT CAAAAGTGCA AAGGTGGATG GGGCCTCCTA TTTCCGGCAC 
AAAGAAAGGC TTCTGCGCAT CTCTATCCGC CACATGGTGA AATCCCAGGT GTTTTACTGG 
ATTGTGTTGA GCCTTGTGGC TCTCAACACT GCCTGTGTGG CCATCGTCCA TCATAACCAG 
CCCCAGTGGC TCACCCATCT CCTCTACTAT GCAGAATTTT TGTTTCTGGG ACTGTTCCTC 
TTGGAGATGT CCCTGAAGAT GTATGGCATG GGACCTCGAC TTTATTTTCA CTCTTCATTC 
AACTGTTTTG ATTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTGGTCTG GGCAATCTTC 
AGACCTGGCA CATCTTTTGG AATCAGTGTC TTACGGGCTC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC ATCTCTGCGA AATTTGGTGG TCTCCTTGAT GAGCTCTATG 
AAGTCTATCA TCAGTTTGCT CTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA 
GGAATGCAGC TGTTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC AGCAAACTTT 
GATACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TAACAGGTGA GGACTGGAAT 
GAGGTGATGT ACAATGGGAT CCGTTCCCAG GGTGGAGTTA GCTCAGGCAT GTGGTCAGCT 
ATCTATTTCA TTGTGCTCAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC 
ATTGCTGTGG ACAATCTGGC CAATGCCCAA GAGCTGACCA AGGATGAACA AGAAGAAGAG 
GAGGCTTTTA ACCAGAAACA TGCATTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCTATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG 
CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCACC ACATGTCGGT GTGGGAGCAG 
CGCACCAGCC AGCTGAGAAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAATAAAGAG 
GAGGCCCCAC CGATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC 
AACGCCCACC CCAGCCTCTA CCGGAGACCT AGGCCTATTG AGGGCCTGGG CCTGGGCCTG 
GAGAAGTGTG AGGAGGAGCG CATCATCAGC CGCGGGGGGT CCCTCAAGGG GGACGGAGGG 
GACCTGTCGA GTGCCCTGGA CAACCAGAGG AGCCCTTTGT CCCTGGGCAA ACGGGAGCCA 
CCATGGCTGG CCAGGCCCTG TCATGGGAAC TGTGATCCGA CCCAGCAGGA GGCTGGGGGA 
GGGGAGACCG TGGTGACCTT CGAGGACCGG GCCAGGCACA GGCAGAGCCA GCGGCGCAGC 
CGGCACCGTC GTGTCAGGAC GGAAGGCAAG GAGCTCTCCT CCACCTCCCG GAGCAGGTCT 
GTCAGCCAGG AGCGGAGTCT GGATGAGGGG GTGCTCACCG ACGGGGAGAA GGAGCGAGAG 
CCCAGGGGCG GCCATGCAGG CAAAGAACCC ACCATCCAAG AAGAAGACAG AGGCCAAGAC 
TTAAGGAGGA CGAACAGTCT GATGGTGCCC AGAGGCTCTG GGCTGGCAGG AGCCCTTGAT 
GAGGCCAACA CACCCCTAGT CCTGCCCCAC CACGATCTGG AAGTGGGGAA GGACACGGTG 
CTGACGGAGC AGGAGCCGGA GGGCAGCAGC GAGCAGGCCC TGCTGGGGGA TGTGCAGTTG 
GACATTGGCA GGGTCATCAG CCAGAGTGAG CCTGACCTCT CCTGCGTCAC AGCCAACACA 
GACAAGGCCA CCACCGACAG CACCAGCGTC ACCGTCGCCA TCCCTGATGT GGGCCCCTTG 
ATGGACTCAA CTGTGATTCA CATTAGCAAC AAAACTGATG GAGAAGCCAG TCCCTTGAAG 
GAGGCTGAGA TCAAAGAGGA GGAGGAGGAA ATGGAGAAGA AGCAGCAGAA GAAGGAGAAG 
CGTGAGACGG GCAAAGCCAT GGTGCCCCAC AGCTCTATGT TCATCTTCAG CACCACCAAC 
CCGATTCGCA GGGCCTGCCA CTACATCGTG AACCTGCGCT ACTTTGAGAT GTGCATTCTC 
CTGGTGATCG CCGCCAGCAG CATTGCCCTG GCAGCAGAGG ACCCCGTCCT GACCAACTCG 
GAGCGCAACA AAGTGGGTTC ACGGGAGCCG TTTGCCAACC TCCGTAATGC CCTGGTCCTG 
AGGTATTTTG ACTATGTTTT CACTGGCGTG TTCACCTTTG AGATGGTTAT AAAGATGATA 
GACCAGGGCT TGATCCTACA GGATGGGTCC TACTTCCGAG ACCTGTGGAA CATCCTGGAC 
TTTGTGGTGG TGGTTGGTGC ATTGGTGGCC TTTGCTTTGG CGAACGCTTT GGGAACAAAC 
AAGGGGCGAG ACATCAAGAC CATCAAATCC CTGAGGGTGC TCCGAGTTCT GAGGCCACTG 
AAGACCATCA AGCGCTTGCC CAAACTCAAG GCTGTCTTTG ACTGTGTGGT GACCTCCTTG 
AAGAATGTCT TCAACATACT CATCGTCTAT AAGCTCTTCA TGTTCATCTT TGCTGTCATT 
GCGGTTCAGC TCTTCAAGGG AAAATTCTTT TATTGCACAG ACAGTTCCAA GGACACAGAG 
AAGGAGTGCA TAGGCAACTA CGTAGATCAT GAAAAAAACA AGATGGAGGT GAAAGGCCGG 
GAATGGAAGC GCCATGAATT CCACTACGAC AACATTATCT GGGCCCTGCT GACCCTCTTC 
ACCGTCTCCA CGGGGGAAGG ATGGCCTCAA GTCTTACAGC ATTCTGTCGA TGTGACAGAG 
GAAGACCGAG GACCAAGCCG CAGCAACCGC ATGGAGATGT CCATCTTCTA TGTGGTCTAC 
TTTGTGGTCT TCCCTTTCTT CTTTGTCAAT ATCTTTGTGG CTCTGATCAT CATCACCTTC 
CAGGAACAAG GGGACAAGAT GATGGAGGAA TGCAGCCTGG AGAAGAACGA GAGGGCGTGC 
ATTGACTTCG CCATCAGTGC CAAACCTCTC ACCCGCTACA TGCCACAGAA CAGGCACACC 
TTCCAGTACC GCGTCTGGCA CTTCGTGGTG TCTCCGTCCT TTGAGTACAC CATCATGGCC 
ATGATCGCCC TGAACACCGT CGTGCTGATG ATGAAGTACT ACTCTGCTCC CTGTACCTAT 
GAACTGGCCC TGAAGTACCT GAATATTGCC TTCACCATGG TGTTTTCCCT GGAATGTGTC 
CTAAAGATCA TCGCTTTTGG CTTCTTGAAC TACTTTCGAG ACACCTGGAA CATCTTTGAC 
TTCATCACTG TGATTGGCAG TATCACAGAA ATCATACTGA CAGACAGCAA GCTGGTAAAC 
ACCAGTGGTT TCAACATGAG CTTTCTGAAG CTCTTCCGTG CCGCCCGCCT CATCAAGCTT 
CTGCGGCAGG GCTACACCAT CCGTATTTTA CTCTGGACCT TCGTGCAGTC CTTTAAGGCC 
CTCCCCTACG TCTGCCTTTT GATTGCCATG CTTTTCTTCA TCTATGCCAT CATTGGGATG 
CAGGTATTTG GAAACATAAA ATTAGATGAG GAGAGTCACA TCAACCGGCA CAACAACTTC 
CGGAGTTTCT TTGGGTCCCT CATGTTACTC TTCAGGAGTG CCACGGGAGA GGCCTGGCAG 
GAGATAATGC TGTCGTGCCT CGGGGAGAAA GGCTGCGAGC CCGACACCAC TGCGCCATCG 
GGGCAGAACG AGAATGAGCG CTGTGGCACC GATCTGGCCT ATGTTTACTT TGTCTCCTTC 
ATCTTCTTCT GCTCTTTCTT GATGCTCAAC TTGTTTGTGG CCGTCATCAT GGACAACTTC 
GAGTACCTGA CTCGGGACTC CTCCATCTTA GGGCCCCACC ACTTGGATGA GTTTGTCCGC 
GTCTGGGCAG AGTACGACCG AGCTGCATGT GGCCGCATCC ATTATACTGA GATGTATGAA 
ATGCTGACTC TCATGTCACC ACCGCTAGGC CTCGGCAAGA GATGTCCCTC CAAAGTGGCA 
TATAAGAGGT TGGTCCTAAT GAATATGCCA GTAGCTGAGG ACATGACAGT CCACTTCACC 
TCCACACTGA TGGCTCTGAT CCGGACAGCC CTGGACATCA AAATCGCCAA AGGTGGTGCA 
GACAGGCAGC AGCTAGACTC GGAGCTGCAA AAGGAGACCC TGGCCATCTG GCCTCACCTG 
TCCCAGAAGA TGCTAGACCT GCTTGTGCCC ATGCCCAAAG CCTCTGACCT GACTGTGGGC 
AAAATCTATG CAGCAATGAT GATCATGGAC TACTATAAAC AGAGTAAGGT GAAGAAACAG 
AGGCAGCAGC TGGAGGAACA GAAAAATGCA CCCATGTTCC AGCGAATGGA GCCCTCATCT 
CTGCCTCAGG AGATCATTGC TAATGCCAAA GCCCTGCCCT ATCTCCAGCA GGACCCCGTT 
TCAGGCCTGA GTGGCCGTAG TGGATACCCT TCGATGAGTC CACTCTCCCC CCAGGAAATA 
TTCCAATTGG CTTGTATGGA TCCAGCCGAT GATGGACAGT TCCAGGAACA TCAGTCTCTG 
GAGCCAGAGG TTAGTGAATT AAAAAGCGTG CAGTCCTCTA ACCATGGCAT CTACCTTCCT 
TCGGACACCC AGGAGCATAC GGGATCTGGG AGGGCATCTT CTATGCCACG CCTGACTGTG 
GATCCCCAGG TGGTGACAGA TACTAGCTCC ATGAGACGTT CATTTTCCAC TATTCGGGAT 
AAGCGTTCAA ATTCCTCCTG GTTGGAGGAA TTCTCCATGG AGAGAAGCAG TGAAAACACC 
TACAAGTCAC GTCGTCGGAG TTACCACTCC TCCCTGAGAC TGTCAGCTCA TCGCCTGAAT 
TCTGACTCAG GCCACAAGTC TGACACCCAC CGCTCAGGGG GTAGAGAGCG GGGACGATCG 
AAAGAGCGAA AGCATCTTCT CTCTCCTGAT GTCTCCCGCT GCAACTCAGA GGAGCGAGGG 
ACCCAGGCCG ACTGGGAGTC CCCAGAGCGC CAACAGTCCA GGTCTCCCAG TGAGGCCAGG 
TCACAGACCC CCAACAGACA GGGCACGGGC TCCCTGAGCG AGAGCTCCAT CCCCTCCATC 
TCTGACACCA GCACCCCAAG ACGAAGTCGC CGGCAGCTCC CACCCGTCCC ACCAAAGCCT 
CGGCCCCTCC TCTCCTACAG CTCCCTGATT CGACACTCCG GGAGCATCTC CCCGCCGGCC 
GACGGAAGCG AGAGGGGCTC CCCGCTGACC CCCCAGGCGC TGGAGAGCAC GGAGGCCGGC 
CTGGCGGAGC CCTCGGGCCC CCGGCACGGG CACGGGCAGG GGCACGGGCA GGGGCACGGG 
CAGCAGAGCC AGCACTGCTC CCCGCAGCGC TACATCTCCG AGCCCTACCT GGCCCTGCAC 
GAAGACTCCC ACGCGTCGGA CTGTGGCGAG GAGGAGACGC TCACCTTCGA GGCCGCCGTG 
GCCACCAGCC TGGGCCGCTC CAACACCATC GGCTCGGCCC CGCCGCTGCG GCACAGCTGG 
CAGATGCCCA ACGGGCACTA TCGGCGGCGG CGGCGCGGGG GGCCGGGGCC GGGCGTGATG 
TGCGGGGCGG TCAGCGACCT CCTGAGTGAC ACGGAAGAAG ACGACAAGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622453.1
CDS	131..7144
Translation	MARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFTNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLGLGLEKCEEERIISRGGSLKGDGGDLSSALDNQRSPLSLGKREPPWLARPCHGNCDPTQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKELSSTSRSRSVSQERSLDEGVLTDGEKEREPRGGHAGKEPTIQEEDRGQDLRRTNSLMVPRGSGLAGALDEANTPLVLPHHDLEVGKDTVLTEQEPEGSSEQALLGDVQLDIGRVISQSEPDLSCVTANTDKATTDSTSVTVAIPDVGPLMDSTVIHISNKTDGEASPLKEAEIKEEEEEMEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVGSREPFANLRNALVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEHQSLEPEVSELKSVQSSNHGIYLPSDTQEHTGSGRASSMPRLTVDPQVVTDTSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERQQSRSPSEARSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHSGSISPPADGSERGSPLTPQALESTEAGLAEPSGPRHGHGQGHGQGHGQQSQHCSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGVMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_005622396.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005622396.1

ORF Insert Sequence:

ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCGGTG CCGGCCTCGG GGCCGGCGGC CGCCTACAAG 
CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TACGCCAAGA AGCTCATCGA CTGGCCGCCG TTTGAGTACA TGATTTTGGC AACCATCATT 
GCCAACTGCA TTGTCTTGGC CCTGGAACAG CATCTTCCTG AGGATGACAA GACCCCGATG 
TCCCGCAGAC TGGAGAAGAC AGAGCCGTAT TTCATTGGGA TCTTTTGCTT TGAAGCCGGT 
ATCAAAATTG TGGCCCTGGG GTTCATCTTT CATAAGGGCT CGTACCTCCG CAATGGCTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCGGG GACTCACTTC 
AACACACACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGAGACC CCTGAAGCTC 
GTGTCAGGGA TTCCTAGCCT GCAGATCGTG TTGAAGTCTA TCATGAAGGC CATGGTGCCT 
CTTCTGCAAA TTGGTCTCCT GCTCTTCTTT GCCATCTTGA TGTTTGCTAT CATTGGCTTA 
GAGTTCTACA GTGGGAAGTT GCACCGAGCA TGTTTCACGA ACAATTCAGG TATTCTGGAA 
GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGCTA CGAATGCCGG 
GACTGGATTG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG 
ACTGTCTTCC AATGCATCAC CATGGAAGGA TGGACCACCG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA CTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTAGTTCT GGGAGTGCTT TCCGGGGAGT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GGAGAGCTTT CATGAAACTG CGGCGGCAGC AGCAGATTGA GCGGGAGCTG 
AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAATAAA 
AATGCTGGGA CGTCAGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAAAG GAGCCGGACA 
GAGGCCATGA CTCGAGACTC CAGTGATGAA CACTGTGTCG ATATCTCCTC TGTGGGCACA 
CCTCTCGCCC GAGCCAGCAT CAAAAGTGCA AAGGTGGATG GGGCCTCCTA TTTCCGGCAC 
AAAGAAAGGC TTCTGCGCAT CTCTATCCGC CACATGGTGA AATCCCAGGT GTTTTACTGG 
ATTGTGTTGA GCCTTGTGGC TCTCAACACT GCCTGTGTGG CCATCGTCCA TCATAACCAG 
CCCCAGTGGC TCACCCATCT CCTCTACTAT GCAGAATTTT TGTTTCTGGG ACTGTTCCTC 
TTGGAGATGT CCCTGAAGAT GTATGGCATG GGACCTCGAC TTTATTTTCA CTCTTCATTC 
AACTGTTTTG ATTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTGGTCTG GGCAATCTTC 
AGACCTGGCA CATCTTTTGG AATCAGTGTC TTACGGGCTC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC ATCTCTGCGA AATTTGGTGG TCTCCTTGAT GAGCTCTATG 
AAGTCTATCA TCAGTTTGCT CTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA 
GGAATGCAGC TGTTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC AGCAAACTTT 
GATACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TAACAGGTGA GGACTGGAAT 
GAGGTGATGT ACAATGGGAT CCGTTCCCAG GGTGGAGTTA GCTCAGGCAT GTGGTCAGCT 
ATCTATTTCA TTGTGCTCAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC 
ATTGCTGTGG ACAATCTGGC CAATGCCCAA GAGCTGACCA AGGATGAACA AGAAGAAGAG 
GAGGCTTTTA ACCAGAAACA TGCATTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCTATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG 
CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCACC ACATGTCGGT GTGGGAGCAG 
CGCACCAGCC AGCTGAGAAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAATAAAGAG 
GAGGCCCCAC CGATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC 
AACGCCCACC CCAGCCTCTA CCGGAGACCT AGGCCTATTG AGGGCCTGGG CCTGGGCCTG 
GAGAAGTGTG AGGAGGAGCG CATCATCAGC CGCGGGGGGT CCCTCAAGGG GGACGGAGGG 
GACCTGTCGA GTGCCCTGGA CAACCAGAGG AGCCCTTTGT CCCTGGGCAA ACGGGAGCCA 
CCATGGCTGG CCAGGCCCTG TCATGGGAAC TGTGATCCGA CCCAGCAGGA GGCTGGGGGA 
GGGGAGACCG TGGTGACCTT CGAGGACCGG GCCAGGCACA GGCAGAGCCA GCGGCGCAGC 
CGGCACCGTC GTGTCAGGAC GGAAGGCAAG GAGCTCTCCT CCACCTCCCG GAGCAGGTCT 
GTCAGCCAGG AGCGGAGTCT GGATGAGGGG GTGCTCACCG ACGGGGAGAA GGAGCGAGAG 
CCCAGGGGCG GCCATGCAGG CAAAGAACCC ACCATCCAAG AAGAAGACAG AGGCCAAGAC 
TTAAGGAGGA CGAACAGTCT GATGGTGCCC AGAGGCTCTG GGCTGGCAGG AGCCCTTGAT 
GAGGCCAACA CACCCCTAGT CCTGCCCCAC CACGATCTGG AAGTGGGGAA GGACACGGTG 
CTGACGGAGC AGGAGCCGGA GGGCAGCAGC GAGCAGGCCC TGCTGGGGGA TGTGCAGTTG 
GACATTGGCA GGGTCATCAG CCAGAGTGAG CCTGACCTCT CCTGCGTCAC AGCCAACACA 
GACAAGGCCA CCACCGACAG CACCAGCGTC ACCGTCGCCA TCCCTGATGT GGGCCCCTTG 
ATGGACTCAA CTGTGATTCA CATTAGCAAC AAAACTGATG GAGAAGCCAG TCCCTTGAAG 
GAGGCTGAGA TCAAAGAGGA GGAGGAGGAA ATGGAGAAGA AGCAGCAGAA GAAGGAGAAG 
CGTGAGACGG GCAAAGCCAT GGTGCCCCAC AGCTCTATGT TCATCTTCAG CACCACCAAC 
CCGATTCGCA GGGCCTGCCA CTACATCGTG AACCTGCGCT ACTTTGAGAT GTGCATTCTC 
CTGGTGATCG CCGCCAGCAG CATTGCCCTG GCAGCAGAGG ACCCCGTCCT GACCAACTCG 
GAGCGCAACA AAGTGGGTTC ACGGGAGCCG TTTGCCAACC TCCGTAATGC CCTGGTCCTG 
AGGTATTTTG ACTATGTTTT CACTGGCGTG TTCACCTTTG AGATGGTTAT AAAGATGATA 
GACCAGGGCT TGATCCTACA GGATGGGTCC TACTTCCGAG ACCTGTGGAA CATCCTGGAC 
TTTGTGGTGG TGGTTGGTGC ATTGGTGGCC TTTGCTTTGG CGAACGCTTT GGGAACAAAC 
AAGGGGCGAG ACATCAAGAC CATCAAATCC CTGAGGGTGC TCCGAGTTCT GAGGCCACTG 
AAGACCATCA AGCGCTTGCC CAAACTCAAG GCTGTCTTTG ACTGTGTGGT GACCTCCTTG 
AAGAATGTCT TCAACATACT CATCGTCTAT AAGCTCTTCA TGTTCATCTT TGCTGTCATT 
GCGGTTCAGC TCTTCAAGGG AAAATTCTTT TATTGCACAG ACAGTTCCAA GGACACAGAG 
AAGGAGTGCA TAGGCAACTA CGTAGATCAT GAAAAAAACA AGATGGAGGT GAAAGGCCGG 
GAATGGAAGC GCCATGAATT CCACTACGAC AACATTATCT GGGCCCTGCT GACCCTCTTC 
ACCGTCTCCA CGGGGGAAGG ATGGCCTCAA GTCTTACAGC ATTCTGTCGA TGTGACAGAG 
GAAGACCGAG GACCAAGCCG CAGCAACCGC ATGGAGATGT CCATCTTCTA TGTGGTCTAC 
TTTGTGGTCT TCCCTTTCTT CTTTGTCAAT ATCTTTGTGG CTCTGATCAT CATCACCTTC 
CAGGAACAAG GGGACAAGAT GATGGAGGAA TGCAGCCTGG AGAAGAACGA GAGGGCGTGC 
ATTGACTTCG CCATCAGTGC CAAACCTCTC ACCCGCTACA TGCCACAGAA CAGGCACACC 
TTCCAGTACC GCGTCTGGCA CTTCGTGGTG TCTCCGTCCT TTGAGTACAC CATCATGGCC 
ATGATCGCCC TGAACACCGT CGTGCTGATG ATGAAGTACT ACTCTGCTCC CTGTACCTAT 
GAACTGGCCC TGAAGTACCT GAATATTGCC TTCACCATGG TGTTTTCCCT GGAATGTGTC 
CTAAAGATCA TCGCTTTTGG CTTCTTGAAC TACTTTCGAG ACACCTGGAA CATCTTTGAC 
TTCATCACTG TGATTGGCAG TATCACAGAA ATCATACTGA CAGACAGCAA GCTGGTAAAC 
ACCAGTGGTT TCAACATGAG CTTTCTGAAG CTCTTCCGTG CCGCCCGCCT CATCAAGCTT 
CTGCGGCAGG GCTACACCAT CCGTATTTTA CTCTGGACCT TCGTGCAGTC CTTTAAGGCC 
CTCCCCTACG TCTGCCTTTT GATTGCCATG CTTTTCTTCA TCTATGCCAT CATTGGGATG 
CAGGTATTTG GAAACATAAA ATTAGATGAG GAGAGTCACA TCAACCGGCA CAACAACTTC 
CGGAGTTTCT TTGGGTCCCT CATGTTACTC TTCAGGAGTG CCACGGGAGA GGCCTGGCAG 
GAGATAATGC TGTCGTGCCT CGGGGAGAAA GGCTGCGAGC CCGACACCAC TGCGCCATCG 
GGGCAGAACG AGAATGAGCG CTGTGGCACC GATCTGGCCT ATGTTTACTT TGTCTCCTTC 
ATCTTCTTCT GCTCTTTCTT GATGCTCAAC TTGTTTGTGG CCGTCATCAT GGACAACTTC 
GAGTACCTGA CTCGGGACTC CTCCATCTTA GGGCCCCACC ACTTGGATGA GTTTGTCCGC 
GTCTGGGCAG AGTACGACCG AGCTGCATGT GGCCGCATCC ATTATACTGA GATGTATGAA 
ATGCTGACTC TCATGTCACC ACCGCTAGGC CTCGGCAAGA GATGTCCCTC CAAAGTGGCA 
TATAAGAGGT TGGTCCTAAT GAATATGCCA GTAGCTGAGG ACATGACAGT CCACTTCACC 
TCCACACTGA TGGCTCTGAT CCGGACAGCC CTGGACATCA AAATCGCCAA AGGTGGTGCA 
GACAGGCAGC AGCTAGACTC GGAGCTGCAA AAGGAGACCC TGGCCATCTG GCCTCACCTG 
TCCCAGAAGA TGCTAGACCT GCTTGTGCCC ATGCCCAAAG CCTCTGACCT GACTGTGGGC 
AAAATCTATG CAGCAATGAT GATCATGGAC TACTATAAAC AGAGTAAGGT GAAGAAACAG 
AGGCAGCAGC TGGAGGAACA GAAAAATGCA CCCATGTTCC AGCGAATGGA GCCCTCATCT 
CTGCCTCAGG AGATCATTGC TAATGCCAAA GCCCTGCCCT ATCTCCAGCA GGACCCCGTT 
TCAGGCCTGA GTGGCCGTAG TGGATACCCT TCGATGAGTC CACTCTCCCC CCAGGAAATA 
TTCCAATTGG CTTGTATGGA TCCAGCCGAT GATGGACAGT TCCAGGAACA TCAGTCTCTG 
GAGCCAGAGG TTAGTGAATT AAAAAGCGTG CAGTCCTCTA ACCATGGCAT CTACCTTCCT 
TCGGACACCC AGGAGCATAC GGGATCTGGG AGGGCATCTT CTATGCCACG CCTGACTGTG 
GATCCCCAGG TGGTGACAGA TACTAGCTCC ATGAGACGTT CATTTTCCAC TATTCGGGAT 
AAGCGTTCAA ATTCCTCCTG GTTGGAGGAA TTCTCCATGG AGAGAAGCAG TGAAAACACC 
TACAAGTCAC GTCGTCGGAG TTACCACTCC TCCCTGAGAC TGTCAGCTCA TCGCCTGAAT 
TCTGACTCAG GCCACAAGTC TGACACCCAC CGCTCAGGGG GTAGAGAGCG GGGACGATCG 
AAAGAGCGAA AGCATCTTCT CTCTCCTGAT GTCTCCCGCT GCAACTCAGA GGAGCGAGGG 
ACCCAGGCCG ACTGGGAGTC CCCAGAGCGC CAACAGTCCA GGTCTCCCAG TGAGGCCAGG 
TCACAGACCC CCAACAGACA GGGCACGGGC TCCCTGAGCG AGAGCTCCAT CCCCTCCATC 
TCTGACACCA GCACCCCAAG ACGAAGTCGC CGGCAGCTCC CACCCGTCCC ACCAAAGCCT 
CGGCCCCTCC TCTCCTACAG CTCCCTGATT CGACACTCCG GGAGCATCTC CCCGCCGGCC 
GACGGAAGCG AGAGGGGCTC CCCGCTGACC CCCCAGGCGC TGGAGAGCAC GGAGGCCGGC 
CTGGCGGAGC CCTCGGGCCC CCGGCACGGG CACGGGCAGG GGCACGGGCA GGGGCACGGG 
CAGCAGAGCC AGCACTGCTC CCCGCAGCGC TACATCTCCG AGCCCTACCT GGCCCTGCAC 
GAAGACTCCC ACGCGTCGGA CTGTGGCGAG GAGGAGACGC TCACCTTCGA GGCCGCCGTG 
GCCACCAGCC TGGGCCGCTC CAACACCATC GGCTCGGCCC CGCCGCTGCG GCACAGCTGG 
CAGATGCCCA ACGGGCACTA TCGGCGGCGG CGGCGCGGGG GGCCGGGGCC GGGCGTGATG 
TGCGGGGCGG TCAGCGACCT CCTGAGTGAC ACGGAAGAAG ACGACAAGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_022420928.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022276636.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe289494
Clone ID Related Accession (Same CDS sequence)	XM_022420928.1
Accession Version	XM_022420928.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGGAC CGAGCCCAGG CCGCAGGCTG CCCGAGCCCG CCCCCTGCCC CGCCGCGGCC 
GGGAGCCCTG AGCCCTGGGC CGCGGGCCGC CGCCGCCACG TCCTGGCCCT GCCTGAGGAG 
GAGGCATGGA GGCCCCAGCA GTGCTCCCTG CTCGAGGCCG ACGCCTCGGA CGAGGTGGGC 
ATGCTGCCGG CCTCCCCGCG CGCCGCCGCC TCCGGCGTCG ACAACTTCTT CCCGGTGCAG 
GAGGGCGAGG GCGCGGGCTG GGAGGGCGGT GCGGCCCTGG ACGCCGGCGC CGGCCCCTTC 
CTCCCGGTGA GCCCCGAGGT GATGAAGCGG CGGCGCGGGG GCCTCATCGA GCAGCGCGAC 
ATCATCAAGG CCCACGAGGC GCACAAGATG CAGAGCACCC CGCAGGCGCG GCGCAAGGAG 
TGGGAGATGG CTCGCTTCGG GGAGGCGGTG GTCGGCAGGC CGGGGTCGGG CGATGGAGAC 
TCGGACCAGA GCAGGAACCG GCAAGGAACC CCGGTGCCGG CCTCGGGGCC GGCGGCCGCC 
TACAAGCAGT CGAAAGCGCA GAGGGCGCGG ACTATGGCTT TGTACAACCC CATTCCCGTC 
CGGCAGAACT GTTTCACCGT CAACAGATCC CTGTTCATCT TCGGAGAAGA TAACATTGTC 
AGGAAATACG CCAAGAAGCT CATCGACTGG CCGCCGTTTG AGTACATGAT TTTGGCAACC 
ATCATTGCCA ACTGCATTGT CTTGGCCCTG GAACAGCATC TTCCTGAGGA TGACAAGACC 
CCGATGTCCC GCAGACTGGA GAAGACAGAG CCGTATTTCA TTGGGATCTT TTGCTTTGAA 
GCCGGTATCA AAATTGTGGC CCTGGGGTTC ATCTTTCATA AGGGCTCGTA CCTCCGCAAT 
GGCTGGAATG TCATGGACTT CATCGTGGTC CTCAGTGGCA TCCTGGCCAC TGCGGGGACT 
CACTTCAACA CACACGTGGA CCTGAGGACC CTCCGGGCTG TGCGTGTCCT GAGACCCCTG 
AAGCTCGTGT CAGGGATTCC TAGCCTGCAG ATCGTGTTGA AGTCTATCAT GAAGGCCATG 
GTGCCTCTTC TGCAAATTGG TCTCCTGCTC TTCTTTGCCA TCTTGATGTT TGCTATCATT 
GGCTTAGAGT TCTACAGTGG GAAGTTGCAC CGAGCATGTT TCACGAACAA TTCAGGTATT 
CTGGAAGGAT TTGACCCCCC TCACCCATGT GGTGTGCAGG GCTGCCCAGC TGGCTACGAA 
TGCCGGGACT GGATTGGCCC CAATGATGGG ATCACCCAGT TTGATAACAT CCTTTTTGCT 
GTGCTGACTG TCTTCCAATG CATCACCATG GAAGGATGGA CCACCGTGCT GTACAATACC 
AATGATGCCT TAGGAGCCAC CTGGAACTGG CTGTACTTCA TCCCCCTCAT CATCATTGGA 
TCCTTCTTTG TTCTCAACCT AGTTCTGGGA GTGCTTTCCG GGGAGTTTGC CAAAGAGAGA 
GAGAGAGTGG AGAACCGGAG AGCTTTCATG AAACTGCGGC GGCAGCAGCA GATTGAGCGG 
GAGCTGAACG GGTACCGCGC CTGGATAGAC AAAGCAGAGG AAGTCATGCT TGCTGAAGAA 
AATAAAAATG CTGGGACGTC AGCCTTAGAA GTGCTTCGAA GGGCAACCAT CAAAAGGAGC 
CGGACAGAGG CCATGACTCG AGACTCCAGT GATGAACACT GTGTCGATAT CTCCTCTGTG 
GGCACACCTC TCGCCCGAGC CAGCATCAAA AGTGCAAAGG TGGATGGGGC CTCCTATTTC 
CGGCACAAAG AAAGGCTTCT GCGCATCTCT ATCCGCCACA TGGTGAAATC CCAGGTGTTT 
TACTGGATTG TGTTGAGCCT TGTGGCTCTC AACACTGCCT GTGTGGCCAT CGTCCATCAT 
AACCAGCCCC AGTGGCTCAC CCATCTCCTC TACTATGCAG AATTTTTGTT TCTGGGACTG 
TTCCTCTTGG AGATGTCCCT GAAGATGTAT GGCATGGGAC CTCGACTTTA TTTTCACTCT 
TCATTCAACT GTTTTGATTT TGGGGTCACA GTGGGCAGCA TCTTTGAAGT GGTCTGGGCA 
ATCTTCAGAC CTGGCACATC TTTTGGAATC AGTGTCTTAC GGGCTCTCCG GCTTCTAAGA 
ATATTTAAAA TAACCAAGTA TTGGGCATCT CTGCGAAATT TGGTGGTCTC CTTGATGAGC 
TCTATGAAGT CTATCATCAG TTTGCTCTTC CTCCTCTTCC TCTTCATCGT TGTCTTTGCT 
CTCCTAGGAA TGCAGCTGTT TGGAGGCAGG TTTAACTTTA ATGATGGGAC TCCTTCAGCA 
AACTTTGATA CCTTCCCTGC AGCCATCATG ACTGTATTCC AGATCCTAAC AGGTGAGGAC 
TGGAATGAGG TGATGTACAA TGGGATCCGT TCCCAGGGTG GAGTTAGCTC AGGCATGTGG 
TCAGCTATCT ATTTCATTGT GCTCACCTTG TTTGGAAACT ACACACTGCT GAATGTGTTC 
TTGGCCATTG CTGTGGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA TGAACAAGAA 
GAAGAGGAGG CTTTTAACCA GAAACATGCA TTGCAGAAGG CCAAGGAGGT CAGCCCGATG 
TCTGCACCCA ACATGCCTTC TATCGAAAGA GACAGAAGGA GAAGACACCA CATGTCGATG 
TGGGAGCCAC GCAGCAGCCA CCTGAGGGAG CGGAGGCGCC GGCACCACAT GTCGGTGTGG 
GAGCAGCGCA CCAGCCAGCT GAGAAGGCAC ATGCAGATGT CCAGCCAGGA GGCCCTCAAT 
AAAGAGGAGG CCCCACCGAT GAACCCCCTC AACCCCCTCA ACCCGCTGAG CCCCCTCAAC 
CCGCTCAACG CCCACCCCAG CCTCTACCGG AGACCTAGGC CTATTGAGGG CCTGGGCCTG 
GGCCTGGAGA AGTGTGAGGA GGAGCGCATC ATCAGCCGCG GGGGGTCCCT CAAGGGGGAC 
GGAGGGGACC TGTCGAGTGC CCTGGACAAC CAGAGGAGCC CTTTGTCCCT GGGCAAACGG 
GAGCCACCAT GGCTGGCCAG GCCCTGTCAT GGGAACTGTG ATCCGACCCA GCAGGAGGCT 
GGGGGAGGGG AGACCGTGGT GACCTTCGAG GACCGGGCCA GGCACAGGCA GAGCCAGCGG 
CGCAGCCGGC ACCGTCGTGT CAGGACGGAA GGCAAGGAGC TCTCCTCCAC CTCCCGGAGC 
AGGTCTGTCA GCCAGGAGCG GAGTCTGGAT GAGGGGGTGC TCACCGACGG GGAGAAGGAG 
CGAGAGCCCA GGGGCGGCCA TGCAGGCAAA GAACCCACCA TCCAAGAAGA AGACAGAGGC 
CAAGACTTAA GGAGGACGAA CAGTCTGATG GTGCCCAGAG GCTCTGGGCT GGCAGGAGCC 
CTTGATGAGG CCAACACACC CCTAGTCCTG CCCCACCACG ATCTGGAAGT GGGGAAGGAC 
ACGGTGCTGA CGGAGCAGGA GCCGGAGGGC AGCAGCGAGC AGGCCCTGCT GGGGGATGTG 
CAGTTGGACA TTGGCAGGGT CATCAGCCAG AGTGAGCCTG ACCTCTCCTG CGTCACAGCC 
AACACAGACA AGGCCACCAC CGACAGCACC AGCGTCACCG TCGCCATCCC TGATGTGGGC 
CCCTTGATGG ACTCAACTGT GATTCACATT AGCAACAAAA CTGATGGAGA AGCCAGTCCC 
TTGAAGGAGG CTGAGATCAA AGAGGAGGAG GAGGAAATGG AGAAGAAGCA GCAGAAGAAG 
GAGAAGCGTG AGACGGGCAA AGCCATGGTG CCCCACAGCT CTATGTTCAT CTTCAGCACC 
ACCAACCCGA TTCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT TGAGATGTGC 
ATTCTCCTGG TGATCGCCGC CAGCAGCATT GCCCTGGCAG CAGAGGACCC CGTCCTGACC 
AACTCGGAGC GCAACAAAGT CCTGAGGTAT TTTGACTATG TTTTCACTGG CGTGTTCACC 
TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATCC TACAGGATGG GTCCTACTTC 
CGAGACCTGT GGAACATCCT GGACTTTGTG GTGGTGGTTG GTGCATTGGT GGCCTTTGCT 
TTGGCGAACG CTTTGGGAAC AAACAAGGGG CGAGACATCA AGACCATCAA ATCCCTGAGG 
GTGCTCCGAG TTCTGAGGCC ACTGAAGACC ATCAAGCGCT TGCCCAAACT CAAGGCTGTC 
TTTGACTGTG TGGTGACCTC CTTGAAGAAT GTCTTCAACA TACTCATCGT CTATAAGCTC 
TTCATGTTCA TCTTTGCTGT CATTGCGGTT CAGCTCTTCA AGGGAAAATT CTTTTATTGC 
ACAGACAGTT CCAAGGACAC AGAGAAGGAG TGCATAGGCA ACTACGTAGA TCATGAAAAA 
AACAAGATGG AGGTGAAAGG CCGGGAATGG AAGCGCCATG AATTCCACTA CGACAACATT 
ATCTGGGCCC TGCTGACCCT CTTCACCGTC TCCACGGGGG AAGGATGGCC TCAAGTCTTA 
CAGCATTCTG TCGATGTGAC AGAGGAAGAC CGAGGACCAA GCCGCAGCAA CCGCATGGAG 
ATGTCCATCT TCTATGTGGT CTACTTTGTG GTCTTCCCTT TCTTCTTTGT CAATATCTTT 
GTGGCTCTGA TCATCATCAC CTTCCAGGAA CAAGGGGACA AGATGATGGA GGAATGCAGC 
CTGGAGAAGA ACGAGAGGGC GTGCATTGAC TTCGCCATCA GTGCCAAACC TCTCACCCGC 
TACATGCCAC AGAACAGGCA CACCTTCCAG TACCGCGTCT GGCACTTCGT GGTGTCTCCG 
TCCTTTGAGT ACACCATCAT GGCCATGATC GCCCTGAACA CCGTCGTGCT GATGATGAAG 
TACTACTCTG CTCCCTGTAC CTATGAACTG GCCCTGAAGT ACCTGAATAT TGCCTTCACC 
ATGGTGTTTT CCCTGGAATG TGTCCTAAAG ATCATCGCTT TTGGCTTCTT GAACTACTTT 
CGAGACACCT GGAACATCTT TGACTTCATC ACTGTGATTG GCAGTATCAC AGAAATCATA 
CTGACAGACA GCAAGCTGGT AAACACCAGT GGTTTCAACA TGAGCTTTCT GAAGCTCTTC 
CGTGCCGCCC GCCTCATCAA GCTTCTGCGG CAGGGCTACA CCATCCGTAT TTTACTCTGG 
ACCTTCGTGC AGTCCTTTAA GGCCCTCCCC TACGTCTGCC TTTTGATTGC CATGCTTTTC 
TTCATCTATG CCATCATTGG GATGCAGGTA TTTGGAAACA TAAAATTAGA TGAGGAGAGT 
CACATCAACC GGCACAACAA CTTCCGGAGT TTCTTTGGGT CCCTCATGTT ACTCTTCAGG 
AGTGCCACGG GAGAGGCCTG GCAGGAGATA ATGCTGTCGT GCCTCGGGGA GAAAGGCTGC 
GAGCCCGACA CCACTGCGCC ATCGGGGCAG AACGAGAATG AGCGCTGTGG CACCGATCTG 
GCCTATGTTT ACTTTGTCTC CTTCATCTTC TTCTGCTCTT TCTTGATGCT CAACTTGTTT 
GTGGCCGTCA TCATGGACAA CTTCGAGTAC CTGACTCGGG ACTCCTCCAT CTTAGGGCCC 
CACCACTTGG ATGAGTTTGT CCGCGTCTGG GCAGAGTACG ACCGAGCTGC ATGTGGCCGC 
ATCCATTATA CTGAGATGTA TGAAATGCTG ACTCTCATGT CACCACCGCT AGGCCTCGGC 
AAGAGATGTC CCTCCAAAGT GGCATATAAG AGGTTGGTCC TAATGAATAT GCCAGTAGCT 
GAGGACATGA CAGTCCACTT CACCTCCACA CTGATGGCTC TGATCCGGAC AGCCCTGGAC 
ATCAAAATCG CCAAAGGTGG TGCAGACAGG CAGCAGCTAG ACTCGGAGCT GCAAAAGGAG 
ACCCTGGCCA TCTGGCCTCA CCTGTCCCAG AAGATGCTAG ACCTGCTTGT GCCCATGCCC 
AAAGCCTCTG ACCTGACTGT GGGCAAAATC TATGCAGCAA TGATGATCAT GGACTACTAT 
AAACAGAGTA AGGTGAAGAA ACAGAGGCAG CAGCTGGAGG AACAGAAAAA TGCACCCATG 
TTCCAGCGAA TGGAGCCCTC ATCTCTGCCT CAGGAGATCA TTGCTAATGC CAAAGCCCTG 
CCCTATCTCC AGCAGGACCC CGTTTCAGGC CTGAGTGGCC GTAGTGGATA CCCTTCGATG 
AGTCCACTCT CCCCCCAGGA AATATTCCAA TTGGCTTGTA TGGATCCAGC CGATGATGGA 
CAGTTCCAGG AACATCAGTC TCTGGAGCCA GAGGTTAGTG AATTAAAAAG CGTGCAGTCC 
TCTAACCATG GCATCTACCT TCCTTCGGAC ACCCAGGAGC ATACGGGATC TGGGAGGGCA 
TCTTCTATGC CACGCCTGAC TGTGGATCCC CAGGTGGTGA CAGATACTAG CTCCATGAGA 
CGTTCATTTT CCACTATTCG GGATAAGCGT TCAAATTCCT CCTGGTTGGA GGAATTCTCC 
ATGGAGAGAA GCAGTGAAAA CACCTACAAG TCACGTCGTC GGAGTTACCA CTCCTCCCTG 
AGACTGTCAG CTCATCGCCT GAATTCTGAC TCAGGCCACA AGTCTGACAC CCACCGCTCA 
GGGGGTAGAG AGCGGGGACG ATCGAAAGAG CGAAAGCATC TTCTCTCTCC TGATGTCTCC 
CGCTGCAACT CAGAGGAGCG AGGGACCCAG GCCGACTGGG AGTCCCCAGA GCGCCAACAG 
TCCAGGTCTC CCAGTGAGGC CAGGTCACAG ACCCCCAACA GACAGGGCAC GGGCTCCCTG 
AGCGAGAGCT CCATCCCCTC CATCTCTGAC ACCAGCACCC CAAGACGAAG TCGCCGGCAG 
CTCCCACCCG TCCCACCAAA GCCTCGGCCC CTCCTCTCCT ACAGCTCCCT GATTCGACAC 
TCCGGGAGCA TCTCCCCGCC GGCCGACGGA AGCGAGAGGG GCTCCCCGCT GACCCCCCAG 
GCGCTGGAGA GCACGGAGGC CGGCCTGGCG GAGCCCTCGG GCCCCCGGCA CGGGCACGGG 
CAGGGGCACG GGCAGGGGCA CGGGCAGCAG AGCCAGCACT GCTCCCCGCA GCGCTACATC 
TCCGAGCCCT ACCTGGCCCT GCACGAAGAC TCCCACGCGT CGGACTGTGG CGAGGAGGAG 
ACGCTCACCT TCGAGGCCGC CGTGGCCACC AGCCTGGGCC GCTCCAACAC CATCGGCTCG 
GCCCCGCCGC TGCGGCACAG CTGGCAGATG CCCAACGGGC ACTATCGGCG GCGGCGGCGC 
GGGGGGCCGG GGCCGGGCGT GATGTGCGGG GCGGTCAGCG ACCTCCTGAG TGACACGGAA 
GAAGACGACA AGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276636.1
CDS	4..7401
Translation	MRGPSPGRRLPEPAPCPAAAGSPEPWAAGRRRHVLALPEEEAWRPQQCSLLEADASDEVGMLPASPRAAASGVDNFFPVQEGEGAGWEGGAALDAGAGPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFTNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLGLGLEKCEEERIISRGGSLKGDGGDLSSALDNQRSPLSLGKREPPWLARPCHGNCDPTQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKELSSTSRSRSVSQERSLDEGVLTDGEKEREPRGGHAGKEPTIQEEDRGQDLRRTNSLMVPRGSGLAGALDEANTPLVLPHHDLEVGKDTVLTEQEPEGSSEQALLGDVQLDIGRVISQSEPDLSCVTANTDKATTDSTSVTVAIPDVGPLMDSTVIHISNKTDGEASPLKEAEIKEEEEEMEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEHQSLEPEVSELKSVQSSNHGIYLPSDTQEHTGSGRASSMPRLTVDPQVVTDTSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERQQSRSPSEARSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHSGSISPPADGSERGSPLTPQALESTEAGLAEPSGPRHGHGQGHGQGHGQQSQHCSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGVMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_022420928.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022420928.1

ORF Insert Sequence:

ATGCGTGGAC CGAGCCCAGG CCGCAGGCTG CCCGAGCCCG CCCCCTGCCC CGCCGCGGCC 
GGGAGCCCTG AGCCCTGGGC CGCGGGCCGC CGCCGCCACG TCCTGGCCCT GCCTGAGGAG 
GAGGCATGGA GGCCCCAGCA GTGCTCCCTG CTCGAGGCCG ACGCCTCGGA CGAGGTGGGC 
ATGCTGCCGG CCTCCCCGCG CGCCGCCGCC TCCGGCGTCG ACAACTTCTT CCCGGTGCAG 
GAGGGCGAGG GCGCGGGCTG GGAGGGCGGT GCGGCCCTGG ACGCCGGCGC CGGCCCCTTC 
CTCCCGGTGA GCCCCGAGGT GATGAAGCGG CGGCGCGGGG GCCTCATCGA GCAGCGCGAC 
ATCATCAAGG CCCACGAGGC GCACAAGATG CAGAGCACCC CGCAGGCGCG GCGCAAGGAG 
TGGGAGATGG CTCGCTTCGG GGAGGCGGTG GTCGGCAGGC CGGGGTCGGG CGATGGAGAC 
TCGGACCAGA GCAGGAACCG GCAAGGAACC CCGGTGCCGG CCTCGGGGCC GGCGGCCGCC 
TACAAGCAGT CGAAAGCGCA GAGGGCGCGG ACTATGGCTT TGTACAACCC CATTCCCGTC 
CGGCAGAACT GTTTCACCGT CAACAGATCC CTGTTCATCT TCGGAGAAGA TAACATTGTC 
AGGAAATACG CCAAGAAGCT CATCGACTGG CCGCCGTTTG AGTACATGAT TTTGGCAACC 
ATCATTGCCA ACTGCATTGT CTTGGCCCTG GAACAGCATC TTCCTGAGGA TGACAAGACC 
CCGATGTCCC GCAGACTGGA GAAGACAGAG CCGTATTTCA TTGGGATCTT TTGCTTTGAA 
GCCGGTATCA AAATTGTGGC CCTGGGGTTC ATCTTTCATA AGGGCTCGTA CCTCCGCAAT 
GGCTGGAATG TCATGGACTT CATCGTGGTC CTCAGTGGCA TCCTGGCCAC TGCGGGGACT 
CACTTCAACA CACACGTGGA CCTGAGGACC CTCCGGGCTG TGCGTGTCCT GAGACCCCTG 
AAGCTCGTGT CAGGGATTCC TAGCCTGCAG ATCGTGTTGA AGTCTATCAT GAAGGCCATG 
GTGCCTCTTC TGCAAATTGG TCTCCTGCTC TTCTTTGCCA TCTTGATGTT TGCTATCATT 
GGCTTAGAGT TCTACAGTGG GAAGTTGCAC CGAGCATGTT TCACGAACAA TTCAGGTATT 
CTGGAAGGAT TTGACCCCCC TCACCCATGT GGTGTGCAGG GCTGCCCAGC TGGCTACGAA 
TGCCGGGACT GGATTGGCCC CAATGATGGG ATCACCCAGT TTGATAACAT CCTTTTTGCT 
GTGCTGACTG TCTTCCAATG CATCACCATG GAAGGATGGA CCACCGTGCT GTACAATACC 
AATGATGCCT TAGGAGCCAC CTGGAACTGG CTGTACTTCA TCCCCCTCAT CATCATTGGA 
TCCTTCTTTG TTCTCAACCT AGTTCTGGGA GTGCTTTCCG GGGAGTTTGC CAAAGAGAGA 
GAGAGAGTGG AGAACCGGAG AGCTTTCATG AAACTGCGGC GGCAGCAGCA GATTGAGCGG 
GAGCTGAACG GGTACCGCGC CTGGATAGAC AAAGCAGAGG AAGTCATGCT TGCTGAAGAA 
AATAAAAATG CTGGGACGTC AGCCTTAGAA GTGCTTCGAA GGGCAACCAT CAAAAGGAGC 
CGGACAGAGG CCATGACTCG AGACTCCAGT GATGAACACT GTGTCGATAT CTCCTCTGTG 
GGCACACCTC TCGCCCGAGC CAGCATCAAA AGTGCAAAGG TGGATGGGGC CTCCTATTTC 
CGGCACAAAG AAAGGCTTCT GCGCATCTCT ATCCGCCACA TGGTGAAATC CCAGGTGTTT 
TACTGGATTG TGTTGAGCCT TGTGGCTCTC AACACTGCCT GTGTGGCCAT CGTCCATCAT 
AACCAGCCCC AGTGGCTCAC CCATCTCCTC TACTATGCAG AATTTTTGTT TCTGGGACTG 
TTCCTCTTGG AGATGTCCCT GAAGATGTAT GGCATGGGAC CTCGACTTTA TTTTCACTCT 
TCATTCAACT GTTTTGATTT TGGGGTCACA GTGGGCAGCA TCTTTGAAGT GGTCTGGGCA 
ATCTTCAGAC CTGGCACATC TTTTGGAATC AGTGTCTTAC GGGCTCTCCG GCTTCTAAGA 
ATATTTAAAA TAACCAAGTA TTGGGCATCT CTGCGAAATT TGGTGGTCTC CTTGATGAGC 
TCTATGAAGT CTATCATCAG TTTGCTCTTC CTCCTCTTCC TCTTCATCGT TGTCTTTGCT 
CTCCTAGGAA TGCAGCTGTT TGGAGGCAGG TTTAACTTTA ATGATGGGAC TCCTTCAGCA 
AACTTTGATA CCTTCCCTGC AGCCATCATG ACTGTATTCC AGATCCTAAC AGGTGAGGAC 
TGGAATGAGG TGATGTACAA TGGGATCCGT TCCCAGGGTG GAGTTAGCTC AGGCATGTGG 
TCAGCTATCT ATTTCATTGT GCTCACCTTG TTTGGAAACT ACACACTGCT GAATGTGTTC 
TTGGCCATTG CTGTGGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA TGAACAAGAA 
GAAGAGGAGG CTTTTAACCA GAAACATGCA TTGCAGAAGG CCAAGGAGGT CAGCCCGATG 
TCTGCACCCA ACATGCCTTC TATCGAAAGA GACAGAAGGA GAAGACACCA CATGTCGATG 
TGGGAGCCAC GCAGCAGCCA CCTGAGGGAG CGGAGGCGCC GGCACCACAT GTCGGTGTGG 
GAGCAGCGCA CCAGCCAGCT GAGAAGGCAC ATGCAGATGT CCAGCCAGGA GGCCCTCAAT 
AAAGAGGAGG CCCCACCGAT GAACCCCCTC AACCCCCTCA ACCCGCTGAG CCCCCTCAAC 
CCGCTCAACG CCCACCCCAG CCTCTACCGG AGACCTAGGC CTATTGAGGG CCTGGGCCTG 
GGCCTGGAGA AGTGTGAGGA GGAGCGCATC ATCAGCCGCG GGGGGTCCCT CAAGGGGGAC 
GGAGGGGACC TGTCGAGTGC CCTGGACAAC CAGAGGAGCC CTTTGTCCCT GGGCAAACGG 
GAGCCACCAT GGCTGGCCAG GCCCTGTCAT GGGAACTGTG ATCCGACCCA GCAGGAGGCT 
GGGGGAGGGG AGACCGTGGT GACCTTCGAG GACCGGGCCA GGCACAGGCA GAGCCAGCGG 
CGCAGCCGGC ACCGTCGTGT CAGGACGGAA GGCAAGGAGC TCTCCTCCAC CTCCCGGAGC 
AGGTCTGTCA GCCAGGAGCG GAGTCTGGAT GAGGGGGTGC TCACCGACGG GGAGAAGGAG 
CGAGAGCCCA GGGGCGGCCA TGCAGGCAAA GAACCCACCA TCCAAGAAGA AGACAGAGGC 
CAAGACTTAA GGAGGACGAA CAGTCTGATG GTGCCCAGAG GCTCTGGGCT GGCAGGAGCC 
CTTGATGAGG CCAACACACC CCTAGTCCTG CCCCACCACG ATCTGGAAGT GGGGAAGGAC 
ACGGTGCTGA CGGAGCAGGA GCCGGAGGGC AGCAGCGAGC AGGCCCTGCT GGGGGATGTG 
CAGTTGGACA TTGGCAGGGT CATCAGCCAG AGTGAGCCTG ACCTCTCCTG CGTCACAGCC 
AACACAGACA AGGCCACCAC CGACAGCACC AGCGTCACCG TCGCCATCCC TGATGTGGGC 
CCCTTGATGG ACTCAACTGT GATTCACATT AGCAACAAAA CTGATGGAGA AGCCAGTCCC 
TTGAAGGAGG CTGAGATCAA AGAGGAGGAG GAGGAAATGG AGAAGAAGCA GCAGAAGAAG 
GAGAAGCGTG AGACGGGCAA AGCCATGGTG CCCCACAGCT CTATGTTCAT CTTCAGCACC 
ACCAACCCGA TTCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT TGAGATGTGC 
ATTCTCCTGG TGATCGCCGC CAGCAGCATT GCCCTGGCAG CAGAGGACCC CGTCCTGACC 
AACTCGGAGC GCAACAAAGT CCTGAGGTAT TTTGACTATG TTTTCACTGG CGTGTTCACC 
TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATCC TACAGGATGG GTCCTACTTC 
CGAGACCTGT GGAACATCCT GGACTTTGTG GTGGTGGTTG GTGCATTGGT GGCCTTTGCT 
TTGGCGAACG CTTTGGGAAC AAACAAGGGG CGAGACATCA AGACCATCAA ATCCCTGAGG 
GTGCTCCGAG TTCTGAGGCC ACTGAAGACC ATCAAGCGCT TGCCCAAACT CAAGGCTGTC 
TTTGACTGTG TGGTGACCTC CTTGAAGAAT GTCTTCAACA TACTCATCGT CTATAAGCTC 
TTCATGTTCA TCTTTGCTGT CATTGCGGTT CAGCTCTTCA AGGGAAAATT CTTTTATTGC 
ACAGACAGTT CCAAGGACAC AGAGAAGGAG TGCATAGGCA ACTACGTAGA TCATGAAAAA 
AACAAGATGG AGGTGAAAGG CCGGGAATGG AAGCGCCATG AATTCCACTA CGACAACATT 
ATCTGGGCCC TGCTGACCCT CTTCACCGTC TCCACGGGGG AAGGATGGCC TCAAGTCTTA 
CAGCATTCTG TCGATGTGAC AGAGGAAGAC CGAGGACCAA GCCGCAGCAA CCGCATGGAG 
ATGTCCATCT TCTATGTGGT CTACTTTGTG GTCTTCCCTT TCTTCTTTGT CAATATCTTT 
GTGGCTCTGA TCATCATCAC CTTCCAGGAA CAAGGGGACA AGATGATGGA GGAATGCAGC 
CTGGAGAAGA ACGAGAGGGC GTGCATTGAC TTCGCCATCA GTGCCAAACC TCTCACCCGC 
TACATGCCAC AGAACAGGCA CACCTTCCAG TACCGCGTCT GGCACTTCGT GGTGTCTCCG 
TCCTTTGAGT ACACCATCAT GGCCATGATC GCCCTGAACA CCGTCGTGCT GATGATGAAG 
TACTACTCTG CTCCCTGTAC CTATGAACTG GCCCTGAAGT ACCTGAATAT TGCCTTCACC 
ATGGTGTTTT CCCTGGAATG TGTCCTAAAG ATCATCGCTT TTGGCTTCTT GAACTACTTT 
CGAGACACCT GGAACATCTT TGACTTCATC ACTGTGATTG GCAGTATCAC AGAAATCATA 
CTGACAGACA GCAAGCTGGT AAACACCAGT GGTTTCAACA TGAGCTTTCT GAAGCTCTTC 
CGTGCCGCCC GCCTCATCAA GCTTCTGCGG CAGGGCTACA CCATCCGTAT TTTACTCTGG 
ACCTTCGTGC AGTCCTTTAA GGCCCTCCCC TACGTCTGCC TTTTGATTGC CATGCTTTTC 
TTCATCTATG CCATCATTGG GATGCAGGTA TTTGGAAACA TAAAATTAGA TGAGGAGAGT 
CACATCAACC GGCACAACAA CTTCCGGAGT TTCTTTGGGT CCCTCATGTT ACTCTTCAGG 
AGTGCCACGG GAGAGGCCTG GCAGGAGATA ATGCTGTCGT GCCTCGGGGA GAAAGGCTGC 
GAGCCCGACA CCACTGCGCC ATCGGGGCAG AACGAGAATG AGCGCTGTGG CACCGATCTG 
GCCTATGTTT ACTTTGTCTC CTTCATCTTC TTCTGCTCTT TCTTGATGCT CAACTTGTTT 
GTGGCCGTCA TCATGGACAA CTTCGAGTAC CTGACTCGGG ACTCCTCCAT CTTAGGGCCC 
CACCACTTGG ATGAGTTTGT CCGCGTCTGG GCAGAGTACG ACCGAGCTGC ATGTGGCCGC 
ATCCATTATA CTGAGATGTA TGAAATGCTG ACTCTCATGT CACCACCGCT AGGCCTCGGC 
AAGAGATGTC CCTCCAAAGT GGCATATAAG AGGTTGGTCC TAATGAATAT GCCAGTAGCT 
GAGGACATGA CAGTCCACTT CACCTCCACA CTGATGGCTC TGATCCGGAC AGCCCTGGAC 
ATCAAAATCG CCAAAGGTGG TGCAGACAGG CAGCAGCTAG ACTCGGAGCT GCAAAAGGAG 
ACCCTGGCCA TCTGGCCTCA CCTGTCCCAG AAGATGCTAG ACCTGCTTGT GCCCATGCCC 
AAAGCCTCTG ACCTGACTGT GGGCAAAATC TATGCAGCAA TGATGATCAT GGACTACTAT 
AAACAGAGTA AGGTGAAGAA ACAGAGGCAG CAGCTGGAGG AACAGAAAAA TGCACCCATG 
TTCCAGCGAA TGGAGCCCTC ATCTCTGCCT CAGGAGATCA TTGCTAATGC CAAAGCCCTG 
CCCTATCTCC AGCAGGACCC CGTTTCAGGC CTGAGTGGCC GTAGTGGATA CCCTTCGATG 
AGTCCACTCT CCCCCCAGGA AATATTCCAA TTGGCTTGTA TGGATCCAGC CGATGATGGA 
CAGTTCCAGG AACATCAGTC TCTGGAGCCA GAGGTTAGTG AATTAAAAAG CGTGCAGTCC 
TCTAACCATG GCATCTACCT TCCTTCGGAC ACCCAGGAGC ATACGGGATC TGGGAGGGCA 
TCTTCTATGC CACGCCTGAC TGTGGATCCC CAGGTGGTGA CAGATACTAG CTCCATGAGA 
CGTTCATTTT CCACTATTCG GGATAAGCGT TCAAATTCCT CCTGGTTGGA GGAATTCTCC 
ATGGAGAGAA GCAGTGAAAA CACCTACAAG TCACGTCGTC GGAGTTACCA CTCCTCCCTG 
AGACTGTCAG CTCATCGCCT GAATTCTGAC TCAGGCCACA AGTCTGACAC CCACCGCTCA 
GGGGGTAGAG AGCGGGGACG ATCGAAAGAG CGAAAGCATC TTCTCTCTCC TGATGTCTCC 
CGCTGCAACT CAGAGGAGCG AGGGACCCAG GCCGACTGGG AGTCCCCAGA GCGCCAACAG 
TCCAGGTCTC CCAGTGAGGC CAGGTCACAG ACCCCCAACA GACAGGGCAC GGGCTCCCTG 
AGCGAGAGCT CCATCCCCTC CATCTCTGAC ACCAGCACCC CAAGACGAAG TCGCCGGCAG 
CTCCCACCCG TCCCACCAAA GCCTCGGCCC CTCCTCTCCT ACAGCTCCCT GATTCGACAC 
TCCGGGAGCA TCTCCCCGCC GGCCGACGGA AGCGAGAGGG GCTCCCCGCT GACCCCCCAG 
GCGCTGGAGA GCACGGAGGC CGGCCTGGCG GAGCCCTCGG GCCCCCGGCA CGGGCACGGG 
CAGGGGCACG GGCAGGGGCA CGGGCAGCAG AGCCAGCACT GCTCCCCGCA GCGCTACATC 
TCCGAGCCCT ACCTGGCCCT GCACGAAGAC TCCCACGCGT CGGACTGTGG CGAGGAGGAG 
ACGCTCACCT TCGAGGCCGC CGTGGCCACC AGCCTGGGCC GCTCCAACAC CATCGGCTCG 
GCCCCGCCGC TGCGGCACAG CTGGCAGATG CCCAACGGGC ACTATCGGCG GCGGCGGCGC 
GGGGGGCCGG GGCCGGGCGT GATGTGCGGG GCGGTCAGCG ACCTCCTGAG TGACACGGAA 
GAAGACGACA AGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_022420929.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022276637.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe289495
Clone ID Related Accession (Same CDS sequence)	XM_022420929.1
Accession Version	XM_022420929.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGGAC CGAGCCCAGG CCGCAGGCTG CCCGAGCCCG CCCCCTGCCC CGCCGCGGCC 
GGGAGCCCTG AGCCCTGGGC CGCGGGCCGC CGCCGCCACG TCCTGGCCCT GCCTGAGGAG 
GAGGCATGGA GGCCCCAGCA GTGCTCCCTG CTCGAGGCCG ACGCCTCGGA CGAGGTGGGC 
ATGCTGCCGG CCTCCCCGCG CGCCGCCGCC TCCGGCGTCG ACAACTTCTT CCCGGTGCAG 
GAGGGCGAGG GCGCGGGCTG GGAGGGCGGT GCGGCCCTGG ACGCCGGCGC CGGCCCCTTC 
CTCCCGGTGA GCCCCGAGGT GATGAAGCGG CGGCGCGGGG GCCTCATCGA GCAGCGCGAC 
ATCATCAAGG CCCACGAGGC GCACAAGATG CAGAGCACCC CGCAGGCGCG GCGCAAGGAG 
TGGGAGATGG CTCGCTTCGG GGAGGCGGTG GTCGGCAGGC CGGGGTCGGG CGATGGAGAC 
TCGGACCAGA GCAGGAACCG GCAAGGAACC CCGGTGCCGG CCTCGGGGCC GGCGGCCGCC 
TACAAGCAGT CGAAAGCGCA GAGGGCGCGG ACTATGGCTT TGTACAACCC CATTCCCGTC 
CGGCAGAACT GTTTCACCGT CAACAGATCC CTGTTCATCT TCGGAGAAGA TAACATTGTC 
AGGAAATACG CCAAGAAGCT CATCGACTGG CCGCCGTTTG AGTACATGAT TTTGGCAACC 
ATCATTGCCA ACTGCATTGT CTTGGCCCTG GAACAGCATC TTCCTGAGGA TGACAAGACC 
CCGATGTCCC GCAGACTGGA GAAGACAGAG CCGTATTTCA TTGGGATCTT TTGCTTTGAA 
GCCGGTATCA AAATTGTGGC CCTGGGGTTC ATCTTTCATA AGGGCTCGTA CCTCCGCAAT 
GGCTGGAATG TCATGGACTT CATCGTGGTC CTCAGTGGCA TCCTGGCCAC TGCGGGGACT 
CACTTCAACA CACACGTGGA CCTGAGGACC CTCCGGGCTG TGCGTGTCCT GAGACCCCTG 
AAGCTCGTGT CAGGGATTCC TAGCCTGCAG ATCGTGTTGA AGTCTATCAT GAAGGCCATG 
GTGCCTCTTC TGCAAATTGG TCTCCTGCTC TTCTTTGCCA TCTTGATGTT TGCTATCATT 
GGCTTAGAGT TCTACAGTGG GAAGTTGCAC CGAGCATGTT TCACGAACAA TTCAGGTATT 
CTGGAAGGAT TTGACCCCCC TCACCCATGT GGTGTGCAGG GCTGCCCAGC TGGCTACGAA 
TGCCGGGACT GGATTGGCCC CAATGATGGG ATCACCCAGT TTGATAACAT CCTTTTTGCT 
GTGCTGACTG TCTTCCAATG CATCACCATG GAAGGATGGA CCACCGTGCT GTACAATACC 
AATGATGCCT TAGGAGCCAC CTGGAACTGG CTGTACTTCA TCCCCCTCAT CATCATTGGA 
TCCTTCTTTG TTCTCAACCT AGTTCTGGGA GTGCTTTCCG GGGAGTTTGC CAAAGAGAGA 
GAGAGAGTGG AGAACCGGAG AGCTTTCATG AAACTGCGGC GGCAGCAGCA GATTGAGCGG 
GAGCTGAACG GGTACCGCGC CTGGATAGAC AAAGCAGAGG AAGTCATGCT TGCTGAAGAA 
AATAAAAATG CTGGGACGTC AGCCTTAGAA GTGCTTCGAA GGGCAACCAT CAAAAGGAGC 
CGGACAGAGG CCATGACTCG AGACTCCAGT GATGAACACT GTGTCGATAT CTCCTCTGTG 
GGCACACCTC TCGCCCGAGC CAGCATCAAA AGTGCAAAGG TGGATGGGGC CTCCTATTTC 
CGGCACAAAG AAAGGCTTCT GCGCATCTCT ATCCGCCACA TGGTGAAATC CCAGGTGTTT 
TACTGGATTG TGTTGAGCCT TGTGGCTCTC AACACTGCCT GTGTGGCCAT CGTCCATCAT 
AACCAGCCCC AGTGGCTCAC CCATCTCCTC TACTATGCAG AATTTTTGTT TCTGGGACTG 
TTCCTCTTGG AGATGTCCCT GAAGATGTAT GGCATGGGAC CTCGACTTTA TTTTCACTCT 
TCATTCAACT GTTTTGATTT TGGGGTCACA GTGGGCAGCA TCTTTGAAGT GGTCTGGGCA 
ATCTTCAGAC CTGGCACATC TTTTGGAATC AGTGTCTTAC GGGCTCTCCG GCTTCTAAGA 
ATATTTAAAA TAACCAAGTA TTGGGCATCT CTGCGAAATT TGGTGGTCTC CTTGATGAGC 
TCTATGAAGT CTATCATCAG TTTGCTCTTC CTCCTCTTCC TCTTCATCGT TGTCTTTGCT 
CTCCTAGGAA TGCAGCTGTT TGGAGGCAGG TTTAACTTTA ATGATGGGAC TCCTTCAGCA 
AACTTTGATA CCTTCCCTGC AGCCATCATG ACTGTATTCC AGATCCTAAC AGGTGAGGAC 
TGGAATGAGG TGATGTACAA TGGGATCCGT TCCCAGGGTG GAGTTAGCTC AGGCATGTGG 
TCAGCTATCT ATTTCATTGT GCTCACCTTG TTTGGAAACT ACACACTGCT GAATGTGTTC 
TTGGCCATTG CTGTGGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA TGAACAAGAA 
GAAGAGGAGG CTTTTAACCA GAAACATGCA TTGCAGAAGG CCAAGGAGGT CAGCCCGATG 
TCTGCACCCA ACATGCCTTC TATCGAAAGA GACAGAAGGA GAAGACACCA CATGTCGATG 
TGGGAGCCAC GCAGCAGCCA CCTGAGGGAG CGGAGGCGCC GGCACCACAT GTCGGTGTGG 
GAGCAGCGCA CCAGCCAGCT GAGAAGGCAC ATGCAGATGT CCAGCCAGGA GGCCCTCAAT 
AAAGAGGAGG CCCCACCGAT GAACCCCCTC AACCCCCTCA ACCCGCTGAG CCCCCTCAAC 
CCGCTCAACG CCCACCCCAG CCTCTACCGG AGACCTAGGC CTATTGAGGG CCTGGGCCTG 
GGCCTGGAGA AGTGTGAGGA GGAGCGCATC ATCAGCCGCG GGGGGTCCCT CAAGGGGGAC 
GGAGGGGACC TGTCGAGTGC CCTGGACAAC CAGAGGAGCC CTTTGTCCCT GGGCAAACGG 
GAGCCACCAT GGCTGGCCAG GCCCTGTCAT GGGAACTGTG ATCCGACCCA GCAGGAGGCT 
GGGGGAGGGG AGACCGTGGT GACCTTCGAG GACCGGGCCA GGCACAGGCA GAGCCAGCGG 
CGCAGCCGGC ACCGTCGTGT CAGGACGGAA GGCAAGGAGC TCTCCTCCAC CTCCCGGAGC 
AGGTCTGTCA GCCAGGAGCG GAGTCTGGAT GAGGGGGTGC TCACCGACGG GGAGAAGGAG 
CGAGAGCCCA GGGGCGGCCA TGCAGGCAAA GAACCCACCA TCCAAGAAGA AGACAGAGGC 
CAAGACTTAA GGAGGACGAA CAGTCTGATG GTGCCCAGAG GCTCTGGGCT GGCAGGAGCC 
CTTGATGAGG CCAACACACC CCTAGTCCTG CCCCACCACG ATCTGGAAGT GGGGAAGGAC 
ACGGTGCTGA CGGAGCAGGA GCCGGAGGGC AGCAGCGAGC AGGCCCTGCT GGGGGATGTG 
CAGTTGGACA TTGGCAGGGT CATCAGCCAG AGTGAGCCTG ACCTCTCCTG CGTCACAGCC 
AACACAGACA AGGCCACCAC CGACAGCACC AGCGTCACCG TCGCCATCCC TGATGTGGGC 
CCCTTGATGG ACTCAACTGT GATTCACATT AGCAACAAAA CTGATGGAGA AGCCAGTCCC 
TTGAAGGAGG CTGAGATCAA AGAGGAGGAG GAGGAAATGG AGAAGAAGCA GCAGAAGAAG 
GAGAAGCGTG AGACGGGCAA AGCCATGGTG CCCCACAGCT CTATGTTCAT CTTCAGCACC 
ACCAACCCGA TTCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT TGAGATGTGC 
ATTCTCCTGG TGATCGCCGC CAGCAGCATT GCCCTGGCAG CAGAGGACCC CGTCCTGACC 
AACTCGGAGC GCAACAAAGT CCTGAGGTAT TTTGACTATG TTTTCACTGG CGTGTTCACC 
TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATCC TACAGGATGG GTCCTACTTC 
CGAGACCTGT GGAACATCCT GGACTTTGTG GTGGTGGTTG GTGCATTGGT GGCCTTTGCT 
TTGGCGAACG CTTTGGGAAC AAACAAGGGG CGAGACATCA AGACCATCAA ATCCCTGAGG 
GTGCTCCGAG TTCTGAGGCC ACTGAAGACC ATCAAGCGCT TGCCCAAACT CAAGGCTGTC 
TTTGACTGTG TGGTGACCTC CTTGAAGAAT GTCTTCAACA TACTCATCGT CTATAAGCTC 
TTCATGTTCA TCTTTGCTGT CATTGCGGTT CAGCTCTTCA AGGGAAAATT CTTTTATTGC 
ACAGACAGTT CCAAGGACAC AGAGAAGGAG TGCATAGGCA ACTACGTAGA TCATGAAAAA 
AACAAGATGG AGGTGAAAGG CCGGGAATGG AAGCGCCATG AATTCCACTA CGACAACATT 
ATCTGGGCCC TGCTGACCCT CTTCACCGTC TCCACGGGGG AAGGATGGCC TCAAGTCTTA 
CAGCATTCTG TCGATGTGAC AGAGGAAGAC CGAGGACCAA GCCGCAGCAA CCGCATGGAG 
ATGTCCATCT TCTATGTGGT CTACTTTGTG GTCTTCCCTT TCTTCTTTGT CAATATCTTT 
GTGGCTCTGA TCATCATCAC CTTCCAGGAA CAAGGGGACA AGATGATGGA GGAATGCAGC 
CTGGAGAAGA ACGAGAGGGC GTGCATTGAC TTCGCCATCA GTGCCAAACC TCTCACCCGC 
TACATGCCAC AGAACAGGCA CACCTTCCAG TACCGCGTCT GGCACTTCGT GGTGTCTCCG 
TCCTTTGAGT ACACCATCAT GGCCATGATC GCCCTGAACA CCGTCGTGCT GATGATGAAG 
TACTACTCTG CTCCCTGTAC CTATGAACTG GCCCTGAAGT ACCTGAATAT TGCCTTCACC 
ATGGTGTTTT CCCTGGAATG TGTCCTAAAG ATCATCGCTT TTGGCTTCTT GAACTACTTT 
CGAGACACCT GGAACATCTT TGACTTCATC ACTGTGATTG GCAGTATCAC AGAAATCATA 
CTGACAGACA GCAAGCTGGT AAACACCAGT GGTTTCAACA TGAGCTTTCT GAAGCTCTTC 
CGTGCCGCCC GCCTCATCAA GCTTCTGCGG CAGGGCTACA CCATCCGTAT TTTACTCTGG 
ACCTTCGTGC AGTCCTTTAA GGCCCTCCCC TACGTCTGCC TTTTGATTGC CATGCTTTTC 
TTCATCTATG CCATCATTGG GATGCAGGTA TTTGGAAACA TAAAATTAGA TGAGGAGAGT 
CACATCAACC GGCACAACAA CTTCCGGAGT TTCTTTGGGT CCCTCATGTT ACTCTTCAGG 
AGTGCCACGG GAGAGGCCTG GCAGGAGATA ATGCTGTCGT GCCTCGGGGA GAAAGGCTGC 
GAGCCCGACA CCACTGCGCC ATCGGGGCAG AACGAGAATG AGCGCTGTGG CACCGATCTG 
GCCTATGTTT ACTTTGTCTC CTTCATCTTC TTCTGCTCTT TCTTGATGCT CAACTTGTTT 
GTGGCCGTCA TCATGGACAA CTTCGAGTAC CTGACTCGGG ACTCCTCCAT CTTAGGGCCC 
CACCACTTGG ATGAGTTTGT CCGCGTCTGG GCAGAGTACG ACCGAGCTGC ATGCGGCAGG 
ATCCCGTATA AGGATATGTA CAAATTAGTG CGGGTGATCT CGCCGCCTCT GGGCCTGGGA 
GAGAACTGCC CCTACAGGGT GGCTTGCAAG AGGTTGGTCC TAATGAATAT GCCAGTAGCT 
GAGGACATGA CAGTCCACTT CACCTCCACA CTGATGGCTC TGATCCGGAC AGCCCTGGAC 
ATCAAAATCG CCAAAGGTGG TGCAGACAGG CAGCAGCTAG ACTCGGAGCT GCAAAAGGAG 
ACCCTGGCCA TCTGGCCTCA CCTGTCCCAG AAGATGCTAG ACCTGCTTGT GCCCATGCCC 
AAAGCCTCTG ACCTGACTGT GGGCAAAATC TATGCAGCAA TGATGATCAT GGACTACTAT 
AAACAGAGTA AGGTGAAGAA ACAGAGGCAG CAGCTGGAGG AACAGAAAAA TGCACCCATG 
TTCCAGCGAA TGGAGCCCTC ATCTCTGCCT CAGGAGATCA TTGCTAATGC CAAAGCCCTG 
CCCTATCTCC AGCAGGACCC CGTTTCAGGC CTGAGTGGCC GTAGTGGATA CCCTTCGATG 
AGTCCACTCT CCCCCCAGGA AATATTCCAA TTGGCTTGTA TGGATCCAGC CGATGATGGA 
CAGTTCCAGG AACATCAGTC TCTGGAGCCA GAGGTTAGTG AATTAAAAAG CGTGCAGTCC 
TCTAACCATG GCATCTACCT TCCTTCGGAC ACCCAGGAGC ATACGGGATC TGGGAGGGCA 
TCTTCTATGC CACGCCTGAC TGTGGATCCC CAGGTGGTGA CAGATACTAG CTCCATGAGA 
CGTTCATTTT CCACTATTCG GGATAAGCGT TCAAATTCCT CCTGGTTGGA GGAATTCTCC 
ATGGAGAGAA GCAGTGAAAA CACCTACAAG TCACGTCGTC GGAGTTACCA CTCCTCCCTG 
AGACTGTCAG CTCATCGCCT GAATTCTGAC TCAGGCCACA AGTCTGACAC CCACCGCTCA 
GGGGGTAGAG AGCGGGGACG ATCGAAAGAG CGAAAGCATC TTCTCTCTCC TGATGTCTCC 
CGCTGCAACT CAGAGGAGCG AGGGACCCAG GCCGACTGGG AGTCCCCAGA GCGCCAACAG 
TCCAGGTCTC CCAGTGAGGC CAGGTCACAG ACCCCCAACA GACAGGGCAC GGGCTCCCTG 
AGCGAGAGCT CCATCCCCTC CATCTCTGAC ACCAGCACCC CAAGACGAAG TCGCCGGCAG 
CTCCCACCCG TCCCACCAAA GCCTCGGCCC CTCCTCTCCT ACAGCTCCCT GATTCGACAC 
TCCGGGAGCA TCTCCCCGCC GGCCGACGGA AGCGAGAGGG GCTCCCCGCT GACCCCCCAG 
GCGCTGGAGA GCACGGAGGC CGGCCTGGCG GAGCCCTCGG GCCCCCGGCA CGGGCACGGG 
CAGGGGCACG GGCAGGGGCA CGGGCAGCAG AGCCAGCACT GCTCCCCGCA GCGCTACATC 
TCCGAGCCCT ACCTGGCCCT GCACGAAGAC TCCCACGCGT CGGACTGTGG CGAGGAGGAG 
ACGCTCACCT TCGAGGCCGC CGTGGCCACC AGCCTGGGCC GCTCCAACAC CATCGGCTCG 
GCCCCGCCGC TGCGGCACAG CTGGCAGATG CCCAACGGGC ACTATCGGCG GCGGCGGCGC 
GGGGGGCCGG GGCCGGGCGT GATGTGCGGG GCGGTCAGCG ACCTCCTGAG TGACACGGAA 
GAAGACGACA AGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276637.1
CDS	5..7402
Translation	MRGPSPGRRLPEPAPCPAAAGSPEPWAAGRRRHVLALPEEEAWRPQQCSLLEADASDEVGMLPASPRAAASGVDNFFPVQEGEGAGWEGGAALDAGAGPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFTNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLGLGLEKCEEERIISRGGSLKGDGGDLSSALDNQRSPLSLGKREPPWLARPCHGNCDPTQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKELSSTSRSRSVSQERSLDEGVLTDGEKEREPRGGHAGKEPTIQEEDRGQDLRRTNSLMVPRGSGLAGALDEANTPLVLPHHDLEVGKDTVLTEQEPEGSSEQALLGDVQLDIGRVISQSEPDLSCVTANTDKATTDSTSVTVAIPDVGPLMDSTVIHISNKTDGEASPLKEAEIKEEEEEMEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIPYKDMYKLVRVISPPLGLGENCPYRVACKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEHQSLEPEVSELKSVQSSNHGIYLPSDTQEHTGSGRASSMPRLTVDPQVVTDTSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERQQSRSPSEARSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHSGSISPPADGSERGSPLTPQALESTEAGLAEPSGPRHGHGQGHGQGHGQQSQHCSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGVMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_022420929.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022420929.1

ORF Insert Sequence:

ATGCGTGGAC CGAGCCCAGG CCGCAGGCTG CCCGAGCCCG CCCCCTGCCC CGCCGCGGCC 
GGGAGCCCTG AGCCCTGGGC CGCGGGCCGC CGCCGCCACG TCCTGGCCCT GCCTGAGGAG 
GAGGCATGGA GGCCCCAGCA GTGCTCCCTG CTCGAGGCCG ACGCCTCGGA CGAGGTGGGC 
ATGCTGCCGG CCTCCCCGCG CGCCGCCGCC TCCGGCGTCG ACAACTTCTT CCCGGTGCAG 
GAGGGCGAGG GCGCGGGCTG GGAGGGCGGT GCGGCCCTGG ACGCCGGCGC CGGCCCCTTC 
CTCCCGGTGA GCCCCGAGGT GATGAAGCGG CGGCGCGGGG GCCTCATCGA GCAGCGCGAC 
ATCATCAAGG CCCACGAGGC GCACAAGATG CAGAGCACCC CGCAGGCGCG GCGCAAGGAG 
TGGGAGATGG CTCGCTTCGG GGAGGCGGTG GTCGGCAGGC CGGGGTCGGG CGATGGAGAC 
TCGGACCAGA GCAGGAACCG GCAAGGAACC CCGGTGCCGG CCTCGGGGCC GGCGGCCGCC 
TACAAGCAGT CGAAAGCGCA GAGGGCGCGG ACTATGGCTT TGTACAACCC CATTCCCGTC 
CGGCAGAACT GTTTCACCGT CAACAGATCC CTGTTCATCT TCGGAGAAGA TAACATTGTC 
AGGAAATACG CCAAGAAGCT CATCGACTGG CCGCCGTTTG AGTACATGAT TTTGGCAACC 
ATCATTGCCA ACTGCATTGT CTTGGCCCTG GAACAGCATC TTCCTGAGGA TGACAAGACC 
CCGATGTCCC GCAGACTGGA GAAGACAGAG CCGTATTTCA TTGGGATCTT TTGCTTTGAA 
GCCGGTATCA AAATTGTGGC CCTGGGGTTC ATCTTTCATA AGGGCTCGTA CCTCCGCAAT 
GGCTGGAATG TCATGGACTT CATCGTGGTC CTCAGTGGCA TCCTGGCCAC TGCGGGGACT 
CACTTCAACA CACACGTGGA CCTGAGGACC CTCCGGGCTG TGCGTGTCCT GAGACCCCTG 
AAGCTCGTGT CAGGGATTCC TAGCCTGCAG ATCGTGTTGA AGTCTATCAT GAAGGCCATG 
GTGCCTCTTC TGCAAATTGG TCTCCTGCTC TTCTTTGCCA TCTTGATGTT TGCTATCATT 
GGCTTAGAGT TCTACAGTGG GAAGTTGCAC CGAGCATGTT TCACGAACAA TTCAGGTATT 
CTGGAAGGAT TTGACCCCCC TCACCCATGT GGTGTGCAGG GCTGCCCAGC TGGCTACGAA 
TGCCGGGACT GGATTGGCCC CAATGATGGG ATCACCCAGT TTGATAACAT CCTTTTTGCT 
GTGCTGACTG TCTTCCAATG CATCACCATG GAAGGATGGA CCACCGTGCT GTACAATACC 
AATGATGCCT TAGGAGCCAC CTGGAACTGG CTGTACTTCA TCCCCCTCAT CATCATTGGA 
TCCTTCTTTG TTCTCAACCT AGTTCTGGGA GTGCTTTCCG GGGAGTTTGC CAAAGAGAGA 
GAGAGAGTGG AGAACCGGAG AGCTTTCATG AAACTGCGGC GGCAGCAGCA GATTGAGCGG 
GAGCTGAACG GGTACCGCGC CTGGATAGAC AAAGCAGAGG AAGTCATGCT TGCTGAAGAA 
AATAAAAATG CTGGGACGTC AGCCTTAGAA GTGCTTCGAA GGGCAACCAT CAAAAGGAGC 
CGGACAGAGG CCATGACTCG AGACTCCAGT GATGAACACT GTGTCGATAT CTCCTCTGTG 
GGCACACCTC TCGCCCGAGC CAGCATCAAA AGTGCAAAGG TGGATGGGGC CTCCTATTTC 
CGGCACAAAG AAAGGCTTCT GCGCATCTCT ATCCGCCACA TGGTGAAATC CCAGGTGTTT 
TACTGGATTG TGTTGAGCCT TGTGGCTCTC AACACTGCCT GTGTGGCCAT CGTCCATCAT 
AACCAGCCCC AGTGGCTCAC CCATCTCCTC TACTATGCAG AATTTTTGTT TCTGGGACTG 
TTCCTCTTGG AGATGTCCCT GAAGATGTAT GGCATGGGAC CTCGACTTTA TTTTCACTCT 
TCATTCAACT GTTTTGATTT TGGGGTCACA GTGGGCAGCA TCTTTGAAGT GGTCTGGGCA 
ATCTTCAGAC CTGGCACATC TTTTGGAATC AGTGTCTTAC GGGCTCTCCG GCTTCTAAGA 
ATATTTAAAA TAACCAAGTA TTGGGCATCT CTGCGAAATT TGGTGGTCTC CTTGATGAGC 
TCTATGAAGT CTATCATCAG TTTGCTCTTC CTCCTCTTCC TCTTCATCGT TGTCTTTGCT 
CTCCTAGGAA TGCAGCTGTT TGGAGGCAGG TTTAACTTTA ATGATGGGAC TCCTTCAGCA 
AACTTTGATA CCTTCCCTGC AGCCATCATG ACTGTATTCC AGATCCTAAC AGGTGAGGAC 
TGGAATGAGG TGATGTACAA TGGGATCCGT TCCCAGGGTG GAGTTAGCTC AGGCATGTGG 
TCAGCTATCT ATTTCATTGT GCTCACCTTG TTTGGAAACT ACACACTGCT GAATGTGTTC 
TTGGCCATTG CTGTGGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA TGAACAAGAA 
GAAGAGGAGG CTTTTAACCA GAAACATGCA TTGCAGAAGG CCAAGGAGGT CAGCCCGATG 
TCTGCACCCA ACATGCCTTC TATCGAAAGA GACAGAAGGA GAAGACACCA CATGTCGATG 
TGGGAGCCAC GCAGCAGCCA CCTGAGGGAG CGGAGGCGCC GGCACCACAT GTCGGTGTGG 
GAGCAGCGCA CCAGCCAGCT GAGAAGGCAC ATGCAGATGT CCAGCCAGGA GGCCCTCAAT 
AAAGAGGAGG CCCCACCGAT GAACCCCCTC AACCCCCTCA ACCCGCTGAG CCCCCTCAAC 
CCGCTCAACG CCCACCCCAG CCTCTACCGG AGACCTAGGC CTATTGAGGG CCTGGGCCTG 
GGCCTGGAGA AGTGTGAGGA GGAGCGCATC ATCAGCCGCG GGGGGTCCCT CAAGGGGGAC 
GGAGGGGACC TGTCGAGTGC CCTGGACAAC CAGAGGAGCC CTTTGTCCCT GGGCAAACGG 
GAGCCACCAT GGCTGGCCAG GCCCTGTCAT GGGAACTGTG ATCCGACCCA GCAGGAGGCT 
GGGGGAGGGG AGACCGTGGT GACCTTCGAG GACCGGGCCA GGCACAGGCA GAGCCAGCGG 
CGCAGCCGGC ACCGTCGTGT CAGGACGGAA GGCAAGGAGC TCTCCTCCAC CTCCCGGAGC 
AGGTCTGTCA GCCAGGAGCG GAGTCTGGAT GAGGGGGTGC TCACCGACGG GGAGAAGGAG 
CGAGAGCCCA GGGGCGGCCA TGCAGGCAAA GAACCCACCA TCCAAGAAGA AGACAGAGGC 
CAAGACTTAA GGAGGACGAA CAGTCTGATG GTGCCCAGAG GCTCTGGGCT GGCAGGAGCC 
CTTGATGAGG CCAACACACC CCTAGTCCTG CCCCACCACG ATCTGGAAGT GGGGAAGGAC 
ACGGTGCTGA CGGAGCAGGA GCCGGAGGGC AGCAGCGAGC AGGCCCTGCT GGGGGATGTG 
CAGTTGGACA TTGGCAGGGT CATCAGCCAG AGTGAGCCTG ACCTCTCCTG CGTCACAGCC 
AACACAGACA AGGCCACCAC CGACAGCACC AGCGTCACCG TCGCCATCCC TGATGTGGGC 
CCCTTGATGG ACTCAACTGT GATTCACATT AGCAACAAAA CTGATGGAGA AGCCAGTCCC 
TTGAAGGAGG CTGAGATCAA AGAGGAGGAG GAGGAAATGG AGAAGAAGCA GCAGAAGAAG 
GAGAAGCGTG AGACGGGCAA AGCCATGGTG CCCCACAGCT CTATGTTCAT CTTCAGCACC 
ACCAACCCGA TTCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT TGAGATGTGC 
ATTCTCCTGG TGATCGCCGC CAGCAGCATT GCCCTGGCAG CAGAGGACCC CGTCCTGACC 
AACTCGGAGC GCAACAAAGT CCTGAGGTAT TTTGACTATG TTTTCACTGG CGTGTTCACC 
TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATCC TACAGGATGG GTCCTACTTC 
CGAGACCTGT GGAACATCCT GGACTTTGTG GTGGTGGTTG GTGCATTGGT GGCCTTTGCT 
TTGGCGAACG CTTTGGGAAC AAACAAGGGG CGAGACATCA AGACCATCAA ATCCCTGAGG 
GTGCTCCGAG TTCTGAGGCC ACTGAAGACC ATCAAGCGCT TGCCCAAACT CAAGGCTGTC 
TTTGACTGTG TGGTGACCTC CTTGAAGAAT GTCTTCAACA TACTCATCGT CTATAAGCTC 
TTCATGTTCA TCTTTGCTGT CATTGCGGTT CAGCTCTTCA AGGGAAAATT CTTTTATTGC 
ACAGACAGTT CCAAGGACAC AGAGAAGGAG TGCATAGGCA ACTACGTAGA TCATGAAAAA 
AACAAGATGG AGGTGAAAGG CCGGGAATGG AAGCGCCATG AATTCCACTA CGACAACATT 
ATCTGGGCCC TGCTGACCCT CTTCACCGTC TCCACGGGGG AAGGATGGCC TCAAGTCTTA 
CAGCATTCTG TCGATGTGAC AGAGGAAGAC CGAGGACCAA GCCGCAGCAA CCGCATGGAG 
ATGTCCATCT TCTATGTGGT CTACTTTGTG GTCTTCCCTT TCTTCTTTGT CAATATCTTT 
GTGGCTCTGA TCATCATCAC CTTCCAGGAA CAAGGGGACA AGATGATGGA GGAATGCAGC 
CTGGAGAAGA ACGAGAGGGC GTGCATTGAC TTCGCCATCA GTGCCAAACC TCTCACCCGC 
TACATGCCAC AGAACAGGCA CACCTTCCAG TACCGCGTCT GGCACTTCGT GGTGTCTCCG 
TCCTTTGAGT ACACCATCAT GGCCATGATC GCCCTGAACA CCGTCGTGCT GATGATGAAG 
TACTACTCTG CTCCCTGTAC CTATGAACTG GCCCTGAAGT ACCTGAATAT TGCCTTCACC 
ATGGTGTTTT CCCTGGAATG TGTCCTAAAG ATCATCGCTT TTGGCTTCTT GAACTACTTT 
CGAGACACCT GGAACATCTT TGACTTCATC ACTGTGATTG GCAGTATCAC AGAAATCATA 
CTGACAGACA GCAAGCTGGT AAACACCAGT GGTTTCAACA TGAGCTTTCT GAAGCTCTTC 
CGTGCCGCCC GCCTCATCAA GCTTCTGCGG CAGGGCTACA CCATCCGTAT TTTACTCTGG 
ACCTTCGTGC AGTCCTTTAA GGCCCTCCCC TACGTCTGCC TTTTGATTGC CATGCTTTTC 
TTCATCTATG CCATCATTGG GATGCAGGTA TTTGGAAACA TAAAATTAGA TGAGGAGAGT 
CACATCAACC GGCACAACAA CTTCCGGAGT TTCTTTGGGT CCCTCATGTT ACTCTTCAGG 
AGTGCCACGG GAGAGGCCTG GCAGGAGATA ATGCTGTCGT GCCTCGGGGA GAAAGGCTGC 
GAGCCCGACA CCACTGCGCC ATCGGGGCAG AACGAGAATG AGCGCTGTGG CACCGATCTG 
GCCTATGTTT ACTTTGTCTC CTTCATCTTC TTCTGCTCTT TCTTGATGCT CAACTTGTTT 
GTGGCCGTCA TCATGGACAA CTTCGAGTAC CTGACTCGGG ACTCCTCCAT CTTAGGGCCC 
CACCACTTGG ATGAGTTTGT CCGCGTCTGG GCAGAGTACG ACCGAGCTGC ATGCGGCAGG 
ATCCCGTATA AGGATATGTA CAAATTAGTG CGGGTGATCT CGCCGCCTCT GGGCCTGGGA 
GAGAACTGCC CCTACAGGGT GGCTTGCAAG AGGTTGGTCC TAATGAATAT GCCAGTAGCT 
GAGGACATGA CAGTCCACTT CACCTCCACA CTGATGGCTC TGATCCGGAC AGCCCTGGAC 
ATCAAAATCG CCAAAGGTGG TGCAGACAGG CAGCAGCTAG ACTCGGAGCT GCAAAAGGAG 
ACCCTGGCCA TCTGGCCTCA CCTGTCCCAG AAGATGCTAG ACCTGCTTGT GCCCATGCCC 
AAAGCCTCTG ACCTGACTGT GGGCAAAATC TATGCAGCAA TGATGATCAT GGACTACTAT 
AAACAGAGTA AGGTGAAGAA ACAGAGGCAG CAGCTGGAGG AACAGAAAAA TGCACCCATG 
TTCCAGCGAA TGGAGCCCTC ATCTCTGCCT CAGGAGATCA TTGCTAATGC CAAAGCCCTG 
CCCTATCTCC AGCAGGACCC CGTTTCAGGC CTGAGTGGCC GTAGTGGATA CCCTTCGATG 
AGTCCACTCT CCCCCCAGGA AATATTCCAA TTGGCTTGTA TGGATCCAGC CGATGATGGA 
CAGTTCCAGG AACATCAGTC TCTGGAGCCA GAGGTTAGTG AATTAAAAAG CGTGCAGTCC 
TCTAACCATG GCATCTACCT TCCTTCGGAC ACCCAGGAGC ATACGGGATC TGGGAGGGCA 
TCTTCTATGC CACGCCTGAC TGTGGATCCC CAGGTGGTGA CAGATACTAG CTCCATGAGA 
CGTTCATTTT CCACTATTCG GGATAAGCGT TCAAATTCCT CCTGGTTGGA GGAATTCTCC 
ATGGAGAGAA GCAGTGAAAA CACCTACAAG TCACGTCGTC GGAGTTACCA CTCCTCCCTG 
AGACTGTCAG CTCATCGCCT GAATTCTGAC TCAGGCCACA AGTCTGACAC CCACCGCTCA 
GGGGGTAGAG AGCGGGGACG ATCGAAAGAG CGAAAGCATC TTCTCTCTCC TGATGTCTCC 
CGCTGCAACT CAGAGGAGCG AGGGACCCAG GCCGACTGGG AGTCCCCAGA GCGCCAACAG 
TCCAGGTCTC CCAGTGAGGC CAGGTCACAG ACCCCCAACA GACAGGGCAC GGGCTCCCTG 
AGCGAGAGCT CCATCCCCTC CATCTCTGAC ACCAGCACCC CAAGACGAAG TCGCCGGCAG 
CTCCCACCCG TCCCACCAAA GCCTCGGCCC CTCCTCTCCT ACAGCTCCCT GATTCGACAC 
TCCGGGAGCA TCTCCCCGCC GGCCGACGGA AGCGAGAGGG GCTCCCCGCT GACCCCCCAG 
GCGCTGGAGA GCACGGAGGC CGGCCTGGCG GAGCCCTCGG GCCCCCGGCA CGGGCACGGG 
CAGGGGCACG GGCAGGGGCA CGGGCAGCAG AGCCAGCACT GCTCCCCGCA GCGCTACATC 
TCCGAGCCCT ACCTGGCCCT GCACGAAGAC TCCCACGCGT CGGACTGTGG CGAGGAGGAG 
ACGCTCACCT TCGAGGCCGC CGTGGCCACC AGCCTGGGCC GCTCCAACAC CATCGGCTCG 
GCCCCGCCGC TGCGGCACAG CTGGCAGATG CCCAACGGGC ACTATCGGCG GCGGCGGCGC 
GGGGGGCCGG GGCCGGGCGT GATGTGCGGG GCGGTCAGCG ACCTCCTGAG TGACACGGAA 
GAAGACGACA AGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_022420930.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022276638.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe289496
Clone ID Related Accession (Same CDS sequence)	XM_022420930.1
Accession Version	XM_022420930.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGGAC CGAGCCCAGG CCGCAGGCTG CCCGAGCCCG CCCCCTGCCC CGCCGCGGCC 
GGGAGCCCTG AGCCCTGGGC CGCGGGCCGC CGCCGCCACG TCCTGGCCCT GCCTGAGGAG 
GAGGCATGGA GGCCCCAGCA GTGCTCCCTG CTCGAGGCCG ACGCCTCGGA CGAGGTGGGC 
ATGCTGCCGG CCTCCCCGCG CGCCGCCGCC TCCGGCGTCG ACAACTTCTT CCCGGTGCAG 
GAGGGCGAGG GCGCGGGCTG GGAGGGCGGT GCGGCCCTGG ACGCCGGCGC CGGCCCCTTC 
CTCCCGGTGA GCCCCGAGGT GATGAAGCGG CGGCGCGGGG GCCTCATCGA GCAGCGCGAC 
ATCATCAAGG CCCACGAGGC GCACAAGATG CAGAGCACCC CGCAGGCGCG GCGCAAGGAG 
TGGGAGATGG CTCGCTTCGG GGAGGCGGTG GTCGGCAGGC CGGGGTCGGG CGATGGAGAC 
TCGGACCAGA GCAGGAACCG GCAAGGAACC CCGGTGCCGG CCTCGGGGCC GGCGGCCGCC 
TACAAGCAGT CGAAAGCGCA GAGGGCGCGG ACTATGGCTT TGTACAACCC CATTCCCGTC 
CGGCAGAACT GTTTCACCGT CAACAGATCC CTGTTCATCT TCGGAGAAGA TAACATTGTC 
AGGAAATACG CCAAGAAGCT CATCGACTGG CCGCCGTTTG AGTACATGAT TTTGGCAACC 
ATCATTGCCA ACTGCATTGT CTTGGCCCTG GAACAGCATC TTCCTGAGGA TGACAAGACC 
CCGATGTCCC GCAGACTGGA GAAGACAGAG CCGTATTTCA TTGGGATCTT TTGCTTTGAA 
GCCGGTATCA AAATTGTGGC CCTGGGGTTC ATCTTTCATA AGGGCTCGTA CCTCCGCAAT 
GGCTGGAATG TCATGGACTT CATCGTGGTC CTCAGTGGCA TCCTGGCCAC TGCGGGGACT 
CACTTCAACA CACACGTGGA CCTGAGGACC CTCCGGGCTG TGCGTGTCCT GAGACCCCTG 
AAGCTCGTGT CAGGGATTCC TAGCCTGCAG ATCGTGTTGA AGTCTATCAT GAAGGCCATG 
GTGCCTCTTC TGCAAATTGG TCTCCTGCTC TTCTTTGCCA TCTTGATGTT TGCTATCATT 
GGCTTAGAGT TCTACAGTGG GAAGTTGCAC CGAGCATGTT TCACGAACAA TTCAGGTATT 
CTGGAAGGAT TTGACCCCCC TCACCCATGT GGTGTGCAGG GCTGCCCAGC TGGCTACGAA 
TGCCGGGACT GGATTGGCCC CAATGATGGG ATCACCCAGT TTGATAACAT CCTTTTTGCT 
GTGCTGACTG TCTTCCAATG CATCACCATG GAAGGATGGA CCACCGTGCT GTACAATACC 
AATGATGCCT TAGGAGCCAC CTGGAACTGG CTGTACTTCA TCCCCCTCAT CATCATTGGA 
TCCTTCTTTG TTCTCAACCT AGTTCTGGGA GTGCTTTCCG GGGAGTTTGC CAAAGAGAGA 
GAGAGAGTGG AGAACCGGAG AGCTTTCATG AAACTGCGGC GGCAGCAGCA GATTGAGCGG 
GAGCTGAACG GGTACCGCGC CTGGATAGAC AAAGCAGAGG AAGTCATGCT TGCTGAAGAA 
AATAAAAATG CTGGGACGTC AGCCTTAGAA GTGCTTCGAA GGGCAACCAT CAAAAGGAGC 
CGGACAGAGG CCATGACTCG AGACTCCAGT GATGAACACT GTGTCGATAT CTCCTCTGTG 
GGCACACCTC TCGCCCGAGC CAGCATCAAA AGTGCAAAGG TGGATGGGGC CTCCTATTTC 
CGGCACAAAG AAAGGCTTCT GCGCATCTCT ATCCGCCACA TGGTGAAATC CCAGGTGTTT 
TACTGGATTG TGTTGAGCCT TGTGGCTCTC AACACTGCCT GTGTGGCCAT CGTCCATCAT 
AACCAGCCCC AGTGGCTCAC CCATCTCCTC TACTATGCAG AATTTTTGTT TCTGGGACTG 
TTCCTCTTGG AGATGTCCCT GAAGATGTAT GGCATGGGAC CTCGACTTTA TTTTCACTCT 
TCATTCAACT GTTTTGATTT TGGGGTCACA GTGGGCAGCA TCTTTGAAGT GGTCTGGGCA 
ATCTTCAGAC CTGGCACATC TTTTGGAATC AGTGTCTTAC GGGCTCTCCG GCTTCTAAGA 
ATATTTAAAA TAACCAAGTA TTGGGCATCT CTGCGAAATT TGGTGGTCTC CTTGATGAGC 
TCTATGAAGT CTATCATCAG TTTGCTCTTC CTCCTCTTCC TCTTCATCGT TGTCTTTGCT 
CTCCTAGGAA TGCAGCTGTT TGGAGGCAGG TTTAACTTTA ATGATGGGAC TCCTTCAGCA 
AACTTTGATA CCTTCCCTGC AGCCATCATG ACTGTATTCC AGATCCTAAC AGGTGAGGAC 
TGGAATGAGG TGATGTACAA TGGGATCCGT TCCCAGGGTG GAGTTAGCTC AGGCATGTGG 
TCAGCTATCT ATTTCATTGT GCTCACCTTG TTTGGAAACT ACACACTGCT GAATGTGTTC 
TTGGCCATTG CTGTGGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA TGAACAAGAA 
GAAGAGGAGG CTTTTAACCA GAAACATGCA TTGCAGAAGG CCAAGGAGGT CAGCCCGATG 
TCTGCACCCA ACATGCCTTC TATCGAAAGA GACAGAAGGA GAAGACACCA CATGTCGATG 
TGGGAGCCAC GCAGCAGCCA CCTGAGGGAG CGGAGGCGCC GGCACCACAT GTCGGTGTGG 
GAGCAGCGCA CCAGCCAGCT GAGAAGGCAC ATGCAGATGT CCAGCCAGGA GGCCCTCAAT 
AAAGAGGAGG CCCCACCGAT GAACCCCCTC AACCCCCTCA ACCCGCTGAG CCCCCTCAAC 
CCGCTCAACG CCCACCCCAG CCTCTACCGG AGACCTAGGC CTATTGAGGG CCTGGGCCTG 
GGCCTGGAGA AGTGTGAGGA GGAGCGCATC ATCAGCCGCG GGGGGTCCCT CAAGGGGGAC 
GGAGGGGACC TGTCGAGTGC CCTGGACAAC CAGAGGAGCC CTTTGTCCCT GGGCAAACGG 
GAGCCACCAT GGCTGGCCAG GCCCTGTCAT GGGAACTGTG ATCCGACCCA GCAGGAGGCT 
GGGGGAGGGG AGACCGTGGT GACCTTCGAG GACCGGGCCA GGCACAGGCA GAGCCAGCGG 
CGCAGCCGGC ACCGTCGTGT CAGGACGGAA GGCAAGGAGC TCTCCTCCAC CTCCCGGAGC 
AGGTCTGTCA GCCAGGAGCG GAGTCTGGAT GAGGGGGTGC TCACCGACGG GGAGAAGGAG 
CGAGAGCCCA GGGGCGGCCA TGCAGGCAAA GAACCCACCA TCCAAGAAGA AGACAGAGGC 
CAAGACTTAA GGAGGACGAA CAGTCTGATG GTGCCCAGAG GCTCTGGGCT GGCAGGAGCC 
CTTGATGAGG CCAACACACC CCTAGTCCTG CCCCACCACG ATCTGGAAGT GGGGAAGGAC 
ACGGTGCTGA CGGAGCAGGA GCCGGAGGGC AGCAGCGAGC AGGCCCTGCT GGGGGATGTG 
CAGTTGGACA TTGGCAGGGT CATCAGCCAG AGTGAGCCTG ACCTCTCCTG CGTCACAGCC 
AACACAGACA AGGCCACCAC CGACAGCACC AGCGTCACCG TCGCCATCCC TGATGTGGGC 
CCCTTGATGG ACTCAACTGT GATTCACATT AGCAACAAAA CTGATGGAGA AGCCAGTCCC 
TTGAAGGAGG CTGAGATCAA AGAGGAGGAG GAGGAAATGG AGAAGAAGCA GCAGAAGAAG 
GAGAAGCGTG AGACGGGCAA AGCCATGGTG CCCCACAGCT CTATGTTCAT CTTCAGCACC 
ACCAACCCGA TTCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT TGAGATGTGC 
ATTCTCCTGG TGATCGCCGC CAGCAGCATT GCCCTGGCAG CAGAGGACCC CGTCCTGACC 
AACTCGGAGC GCAACAAAGT CCTGAGGTAT TTTGACTATG TTTTCACTGG CGTGTTCACC 
TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATCC TACAGGATGG GTCCTACTTC 
CGAGACCTGT GGAACATCCT GGACTTTGTG GTGGTGGTTG GTGCATTGGT GGCCTTTGCT 
TTGGCAACAA ACAAGGGGCG AGACATCAAG ACCATCAAAT CCCTGAGGGT GCTCCGAGTT 
CTGAGGCCAC TGAAGACCAT CAAGCGCTTG CCCAAACTCA AGGCTGTCTT TGACTGTGTG 
GTGACCTCCT TGAAGAATGT CTTCAACATA CTCATCGTCT ATAAGCTCTT CATGTTCATC 
TTTGCTGTCA TTGCGGTTCA GCTCTTCAAG GGAAAATTCT TTTATTGCAC AGACAGTTCC 
AAGGACACAG AGAAGGAGTG CATAGGCAAC TACGTAGATC ATGAAAAAAA CAAGATGGAG 
GTGAAAGGCC GGGAATGGAA GCGCCATGAA TTCCACTACG ACAACATTAT CTGGGCCCTG 
CTGACCCTCT TCACCGTCTC CACGGGGGAA GGATGGCCTC AAGTCTTACA GCATTCTGTC 
GATGTGACAG AGGAAGACCG AGGACCAAGC CGCAGCAACC GCATGGAGAT GTCCATCTTC 
TATGTGGTCT ACTTTGTGGT CTTCCCTTTC TTCTTTGTCA ATATCTTTGT GGCTCTGATC 
ATCATCACCT TCCAGGAACA AGGGGACAAG ATGATGGAGG AATGCAGCCT GGAGAAGAAC 
GAGAGGGCGT GCATTGACTT CGCCATCAGT GCCAAACCTC TCACCCGCTA CATGCCACAG 
AACAGGCACA CCTTCCAGTA CCGCGTCTGG CACTTCGTGG TGTCTCCGTC CTTTGAGTAC 
ACCATCATGG CCATGATCGC CCTGAACACC GTCGTGCTGA TGATGAAGTA CTACTCTGCT 
CCCTGTACCT ATGAACTGGC CCTGAAGTAC CTGAATATTG CCTTCACCAT GGTGTTTTCC 
CTGGAATGTG TCCTAAAGAT CATCGCTTTT GGCTTCTTGA ACTACTTTCG AGACACCTGG 
AACATCTTTG ACTTCATCAC TGTGATTGGC AGTATCACAG AAATCATACT GACAGACAGC 
AAGCTGGTAA ACACCAGTGG TTTCAACATG AGCTTTCTGA AGCTCTTCCG TGCCGCCCGC 
CTCATCAAGC TTCTGCGGCA GGGCTACACC ATCCGTATTT TACTCTGGAC CTTCGTGCAG 
TCCTTTAAGG CCCTCCCCTA CGTCTGCCTT TTGATTGCCA TGCTTTTCTT CATCTATGCC 
ATCATTGGGA TGCAGGTATT TGGAAACATA AAATTAGATG AGGAGAGTCA CATCAACCGG 
CACAACAACT TCCGGAGTTT CTTTGGGTCC CTCATGTTAC TCTTCAGGAG TGCCACGGGA 
GAGGCCTGGC AGGAGATAAT GCTGTCGTGC CTCGGGGAGA AAGGCTGCGA GCCCGACACC 
ACTGCGCCAT CGGGGCAGAA CGAGAATGAG CGCTGTGGCA CCGATCTGGC CTATGTTTAC 
TTTGTCTCCT TCATCTTCTT CTGCTCTTTC TTGATGCTCA ACTTGTTTGT GGCCGTCATC 
ATGGACAACT TCGAGTACCT GACTCGGGAC TCCTCCATCT TAGGGCCCCA CCACTTGGAT 
GAGTTTGTCC GCGTCTGGGC AGAGTACGAC CGAGCTGCAT GTGGCCGCAT CCATTATACT 
GAGATGTATG AAATGCTGAC TCTCATGTCA CCACCGCTAG GCCTCGGCAA GAGATGTCCC 
TCCAAAGTGG CATATAAGAG GTTGGTCCTA ATGAATATGC CAGTAGCTGA GGACATGACA 
GTCCACTTCA CCTCCACACT GATGGCTCTG ATCCGGACAG CCCTGGACAT CAAAATCGCC 
AAAGGTGGTG CAGACAGGCA GCAGCTAGAC TCGGAGCTGC AAAAGGAGAC CCTGGCCATC 
TGGCCTCACC TGTCCCAGAA GATGCTAGAC CTGCTTGTGC CCATGCCCAA AGCCTCTGAC 
CTGACTGTGG GCAAAATCTA TGCAGCAATG ATGATCATGG ACTACTATAA ACAGAGTAAG 
GTGAAGAAAC AGAGGCAGCA GCTGGAGGAA CAGAAAAATG CACCCATGTT CCAGCGAATG 
GAGCCCTCAT CTCTGCCTCA GGAGATCATT GCTAATGCCA AAGCCCTGCC CTATCTCCAG 
CAGGACCCCG TTTCAGGCCT GAGTGGCCGT AGTGGATACC CTTCGATGAG TCCACTCTCC 
CCCCAGGAAA TATTCCAATT GGCTTGTATG GATCCAGCCG ATGATGGACA GTTCCAGGAA 
CATCAGTCTC TGGAGCCAGA GGTTAGTGAA TTAAAAAGCG TGCAGTCCTC TAACCATGGC 
ATCTACCTTC CTTCGGACAC CCAGGAGCAT ACGGGATCTG GGAGGGCATC TTCTATGCCA 
CGCCTGACTG TGGATCCCCA GGTGGTGACA GATACTAGCT CCATGAGACG TTCATTTTCC 
ACTATTCGGG ATAAGCGTTC AAATTCCTCC TGGTTGGAGG AATTCTCCAT GGAGAGAAGC 
AGTGAAAACA CCTACAAGTC ACGTCGTCGG AGTTACCACT CCTCCCTGAG ACTGTCAGCT 
CATCGCCTGA ATTCTGACTC AGGCCACAAG TCTGACACCC ACCGCTCAGG GGGTAGAGAG 
CGGGGACGAT CGAAAGAGCG AAAGCATCTT CTCTCTCCTG ATGTCTCCCG CTGCAACTCA 
GAGGAGCGAG GGACCCAGGC CGACTGGGAG TCCCCAGAGC GCCAACAGTC CAGGTCTCCC 
AGTGAGGCCA GGTCACAGAC CCCCAACAGA CAGGGCACGG GCTCCCTGAG CGAGAGCTCC 
ATCCCCTCCA TCTCTGACAC CAGCACCCCA AGACGAAGTC GCCGGCAGCT CCCACCCGTC 
CCACCAAAGC CTCGGCCCCT CCTCTCCTAC AGCTCCCTGA TTCGACACTC CGGGAGCATC 
TCCCCGCCGG CCGACGGAAG CGAGAGGGGC TCCCCGCTGA CCCCCCAGGC GCTGGAGAGC 
ACGGAGGCCG GCCTGGCGGA GCCCTCGGGC CCCCGGCACG GGCACGGGCA GGGGCACGGG 
CAGGGGCACG GGCAGCAGAG CCAGCACTGC TCCCCGCAGC GCTACATCTC CGAGCCCTAC 
CTGGCCCTGC ACGAAGACTC CCACGCGTCG GACTGTGGCG AGGAGGAGAC GCTCACCTTC 
GAGGCCGCCG TGGCCACCAG CCTGGGCCGC TCCAACACCA TCGGCTCGGC CCCGCCGCTG 
CGGCACAGCT GGCAGATGCC CAACGGGCAC TATCGGCGGC GGCGGCGCGG GGGGCCGGGG 
CCGGGCGTGA TGTGCGGGGC GGTCAGCGAC CTCCTGAGTG ACACGGAAGA AGACGACAAG 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276638.1
CDS	5..7390
Translation	MRGPSPGRRLPEPAPCPAAAGSPEPWAAGRRRHVLALPEEEAWRPQQCSLLEADASDEVGMLPASPRAAASGVDNFFPVQEGEGAGWEGGAALDAGAGPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFTNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLGLGLEKCEEERIISRGGSLKGDGGDLSSALDNQRSPLSLGKREPPWLARPCHGNCDPTQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKELSSTSRSRSVSQERSLDEGVLTDGEKEREPRGGHAGKEPTIQEEDRGQDLRRTNSLMVPRGSGLAGALDEANTPLVLPHHDLEVGKDTVLTEQEPEGSSEQALLGDVQLDIGRVISQSEPDLSCVTANTDKATTDSTSVTVAIPDVGPLMDSTVIHISNKTDGEASPLKEAEIKEEEEEMEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALATNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEHQSLEPEVSELKSVQSSNHGIYLPSDTQEHTGSGRASSMPRLTVDPQVVTDTSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERQQSRSPSEARSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHSGSISPPADGSERGSPLTPQALESTEAGLAEPSGPRHGHGQGHGQGHGQQSQHCSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGVMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_022420930.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022420930.1

ORF Insert Sequence:

ATGCGTGGAC CGAGCCCAGG CCGCAGGCTG CCCGAGCCCG CCCCCTGCCC CGCCGCGGCC 
GGGAGCCCTG AGCCCTGGGC CGCGGGCCGC CGCCGCCACG TCCTGGCCCT GCCTGAGGAG 
GAGGCATGGA GGCCCCAGCA GTGCTCCCTG CTCGAGGCCG ACGCCTCGGA CGAGGTGGGC 
ATGCTGCCGG CCTCCCCGCG CGCCGCCGCC TCCGGCGTCG ACAACTTCTT CCCGGTGCAG 
GAGGGCGAGG GCGCGGGCTG GGAGGGCGGT GCGGCCCTGG ACGCCGGCGC CGGCCCCTTC 
CTCCCGGTGA GCCCCGAGGT GATGAAGCGG CGGCGCGGGG GCCTCATCGA GCAGCGCGAC 
ATCATCAAGG CCCACGAGGC GCACAAGATG CAGAGCACCC CGCAGGCGCG GCGCAAGGAG 
TGGGAGATGG CTCGCTTCGG GGAGGCGGTG GTCGGCAGGC CGGGGTCGGG CGATGGAGAC 
TCGGACCAGA GCAGGAACCG GCAAGGAACC CCGGTGCCGG CCTCGGGGCC GGCGGCCGCC 
TACAAGCAGT CGAAAGCGCA GAGGGCGCGG ACTATGGCTT TGTACAACCC CATTCCCGTC 
CGGCAGAACT GTTTCACCGT CAACAGATCC CTGTTCATCT TCGGAGAAGA TAACATTGTC 
AGGAAATACG CCAAGAAGCT CATCGACTGG CCGCCGTTTG AGTACATGAT TTTGGCAACC 
ATCATTGCCA ACTGCATTGT CTTGGCCCTG GAACAGCATC TTCCTGAGGA TGACAAGACC 
CCGATGTCCC GCAGACTGGA GAAGACAGAG CCGTATTTCA TTGGGATCTT TTGCTTTGAA 
GCCGGTATCA AAATTGTGGC CCTGGGGTTC ATCTTTCATA AGGGCTCGTA CCTCCGCAAT 
GGCTGGAATG TCATGGACTT CATCGTGGTC CTCAGTGGCA TCCTGGCCAC TGCGGGGACT 
CACTTCAACA CACACGTGGA CCTGAGGACC CTCCGGGCTG TGCGTGTCCT GAGACCCCTG 
AAGCTCGTGT CAGGGATTCC TAGCCTGCAG ATCGTGTTGA AGTCTATCAT GAAGGCCATG 
GTGCCTCTTC TGCAAATTGG TCTCCTGCTC TTCTTTGCCA TCTTGATGTT TGCTATCATT 
GGCTTAGAGT TCTACAGTGG GAAGTTGCAC CGAGCATGTT TCACGAACAA TTCAGGTATT 
CTGGAAGGAT TTGACCCCCC TCACCCATGT GGTGTGCAGG GCTGCCCAGC TGGCTACGAA 
TGCCGGGACT GGATTGGCCC CAATGATGGG ATCACCCAGT TTGATAACAT CCTTTTTGCT 
GTGCTGACTG TCTTCCAATG CATCACCATG GAAGGATGGA CCACCGTGCT GTACAATACC 
AATGATGCCT TAGGAGCCAC CTGGAACTGG CTGTACTTCA TCCCCCTCAT CATCATTGGA 
TCCTTCTTTG TTCTCAACCT AGTTCTGGGA GTGCTTTCCG GGGAGTTTGC CAAAGAGAGA 
GAGAGAGTGG AGAACCGGAG AGCTTTCATG AAACTGCGGC GGCAGCAGCA GATTGAGCGG 
GAGCTGAACG GGTACCGCGC CTGGATAGAC AAAGCAGAGG AAGTCATGCT TGCTGAAGAA 
AATAAAAATG CTGGGACGTC AGCCTTAGAA GTGCTTCGAA GGGCAACCAT CAAAAGGAGC 
CGGACAGAGG CCATGACTCG AGACTCCAGT GATGAACACT GTGTCGATAT CTCCTCTGTG 
GGCACACCTC TCGCCCGAGC CAGCATCAAA AGTGCAAAGG TGGATGGGGC CTCCTATTTC 
CGGCACAAAG AAAGGCTTCT GCGCATCTCT ATCCGCCACA TGGTGAAATC CCAGGTGTTT 
TACTGGATTG TGTTGAGCCT TGTGGCTCTC AACACTGCCT GTGTGGCCAT CGTCCATCAT 
AACCAGCCCC AGTGGCTCAC CCATCTCCTC TACTATGCAG AATTTTTGTT TCTGGGACTG 
TTCCTCTTGG AGATGTCCCT GAAGATGTAT GGCATGGGAC CTCGACTTTA TTTTCACTCT 
TCATTCAACT GTTTTGATTT TGGGGTCACA GTGGGCAGCA TCTTTGAAGT GGTCTGGGCA 
ATCTTCAGAC CTGGCACATC TTTTGGAATC AGTGTCTTAC GGGCTCTCCG GCTTCTAAGA 
ATATTTAAAA TAACCAAGTA TTGGGCATCT CTGCGAAATT TGGTGGTCTC CTTGATGAGC 
TCTATGAAGT CTATCATCAG TTTGCTCTTC CTCCTCTTCC TCTTCATCGT TGTCTTTGCT 
CTCCTAGGAA TGCAGCTGTT TGGAGGCAGG TTTAACTTTA ATGATGGGAC TCCTTCAGCA 
AACTTTGATA CCTTCCCTGC AGCCATCATG ACTGTATTCC AGATCCTAAC AGGTGAGGAC 
TGGAATGAGG TGATGTACAA TGGGATCCGT TCCCAGGGTG GAGTTAGCTC AGGCATGTGG 
TCAGCTATCT ATTTCATTGT GCTCACCTTG TTTGGAAACT ACACACTGCT GAATGTGTTC 
TTGGCCATTG CTGTGGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA TGAACAAGAA 
GAAGAGGAGG CTTTTAACCA GAAACATGCA TTGCAGAAGG CCAAGGAGGT CAGCCCGATG 
TCTGCACCCA ACATGCCTTC TATCGAAAGA GACAGAAGGA GAAGACACCA CATGTCGATG 
TGGGAGCCAC GCAGCAGCCA CCTGAGGGAG CGGAGGCGCC GGCACCACAT GTCGGTGTGG 
GAGCAGCGCA CCAGCCAGCT GAGAAGGCAC ATGCAGATGT CCAGCCAGGA GGCCCTCAAT 
AAAGAGGAGG CCCCACCGAT GAACCCCCTC AACCCCCTCA ACCCGCTGAG CCCCCTCAAC 
CCGCTCAACG CCCACCCCAG CCTCTACCGG AGACCTAGGC CTATTGAGGG CCTGGGCCTG 
GGCCTGGAGA AGTGTGAGGA GGAGCGCATC ATCAGCCGCG GGGGGTCCCT CAAGGGGGAC 
GGAGGGGACC TGTCGAGTGC CCTGGACAAC CAGAGGAGCC CTTTGTCCCT GGGCAAACGG 
GAGCCACCAT GGCTGGCCAG GCCCTGTCAT GGGAACTGTG ATCCGACCCA GCAGGAGGCT 
GGGGGAGGGG AGACCGTGGT GACCTTCGAG GACCGGGCCA GGCACAGGCA GAGCCAGCGG 
CGCAGCCGGC ACCGTCGTGT CAGGACGGAA GGCAAGGAGC TCTCCTCCAC CTCCCGGAGC 
AGGTCTGTCA GCCAGGAGCG GAGTCTGGAT GAGGGGGTGC TCACCGACGG GGAGAAGGAG 
CGAGAGCCCA GGGGCGGCCA TGCAGGCAAA GAACCCACCA TCCAAGAAGA AGACAGAGGC 
CAAGACTTAA GGAGGACGAA CAGTCTGATG GTGCCCAGAG GCTCTGGGCT GGCAGGAGCC 
CTTGATGAGG CCAACACACC CCTAGTCCTG CCCCACCACG ATCTGGAAGT GGGGAAGGAC 
ACGGTGCTGA CGGAGCAGGA GCCGGAGGGC AGCAGCGAGC AGGCCCTGCT GGGGGATGTG 
CAGTTGGACA TTGGCAGGGT CATCAGCCAG AGTGAGCCTG ACCTCTCCTG CGTCACAGCC 
AACACAGACA AGGCCACCAC CGACAGCACC AGCGTCACCG TCGCCATCCC TGATGTGGGC 
CCCTTGATGG ACTCAACTGT GATTCACATT AGCAACAAAA CTGATGGAGA AGCCAGTCCC 
TTGAAGGAGG CTGAGATCAA AGAGGAGGAG GAGGAAATGG AGAAGAAGCA GCAGAAGAAG 
GAGAAGCGTG AGACGGGCAA AGCCATGGTG CCCCACAGCT CTATGTTCAT CTTCAGCACC 
ACCAACCCGA TTCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT TGAGATGTGC 
ATTCTCCTGG TGATCGCCGC CAGCAGCATT GCCCTGGCAG CAGAGGACCC CGTCCTGACC 
AACTCGGAGC GCAACAAAGT CCTGAGGTAT TTTGACTATG TTTTCACTGG CGTGTTCACC 
TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATCC TACAGGATGG GTCCTACTTC 
CGAGACCTGT GGAACATCCT GGACTTTGTG GTGGTGGTTG GTGCATTGGT GGCCTTTGCT 
TTGGCAACAA ACAAGGGGCG AGACATCAAG ACCATCAAAT CCCTGAGGGT GCTCCGAGTT 
CTGAGGCCAC TGAAGACCAT CAAGCGCTTG CCCAAACTCA AGGCTGTCTT TGACTGTGTG 
GTGACCTCCT TGAAGAATGT CTTCAACATA CTCATCGTCT ATAAGCTCTT CATGTTCATC 
TTTGCTGTCA TTGCGGTTCA GCTCTTCAAG GGAAAATTCT TTTATTGCAC AGACAGTTCC 
AAGGACACAG AGAAGGAGTG CATAGGCAAC TACGTAGATC ATGAAAAAAA CAAGATGGAG 
GTGAAAGGCC GGGAATGGAA GCGCCATGAA TTCCACTACG ACAACATTAT CTGGGCCCTG 
CTGACCCTCT TCACCGTCTC CACGGGGGAA GGATGGCCTC AAGTCTTACA GCATTCTGTC 
GATGTGACAG AGGAAGACCG AGGACCAAGC CGCAGCAACC GCATGGAGAT GTCCATCTTC 
TATGTGGTCT ACTTTGTGGT CTTCCCTTTC TTCTTTGTCA ATATCTTTGT GGCTCTGATC 
ATCATCACCT TCCAGGAACA AGGGGACAAG ATGATGGAGG AATGCAGCCT GGAGAAGAAC 
GAGAGGGCGT GCATTGACTT CGCCATCAGT GCCAAACCTC TCACCCGCTA CATGCCACAG 
AACAGGCACA CCTTCCAGTA CCGCGTCTGG CACTTCGTGG TGTCTCCGTC CTTTGAGTAC 
ACCATCATGG CCATGATCGC CCTGAACACC GTCGTGCTGA TGATGAAGTA CTACTCTGCT 
CCCTGTACCT ATGAACTGGC CCTGAAGTAC CTGAATATTG CCTTCACCAT GGTGTTTTCC 
CTGGAATGTG TCCTAAAGAT CATCGCTTTT GGCTTCTTGA ACTACTTTCG AGACACCTGG 
AACATCTTTG ACTTCATCAC TGTGATTGGC AGTATCACAG AAATCATACT GACAGACAGC 
AAGCTGGTAA ACACCAGTGG TTTCAACATG AGCTTTCTGA AGCTCTTCCG TGCCGCCCGC 
CTCATCAAGC TTCTGCGGCA GGGCTACACC ATCCGTATTT TACTCTGGAC CTTCGTGCAG 
TCCTTTAAGG CCCTCCCCTA CGTCTGCCTT TTGATTGCCA TGCTTTTCTT CATCTATGCC 
ATCATTGGGA TGCAGGTATT TGGAAACATA AAATTAGATG AGGAGAGTCA CATCAACCGG 
CACAACAACT TCCGGAGTTT CTTTGGGTCC CTCATGTTAC TCTTCAGGAG TGCCACGGGA 
GAGGCCTGGC AGGAGATAAT GCTGTCGTGC CTCGGGGAGA AAGGCTGCGA GCCCGACACC 
ACTGCGCCAT CGGGGCAGAA CGAGAATGAG CGCTGTGGCA CCGATCTGGC CTATGTTTAC 
TTTGTCTCCT TCATCTTCTT CTGCTCTTTC TTGATGCTCA ACTTGTTTGT GGCCGTCATC 
ATGGACAACT TCGAGTACCT GACTCGGGAC TCCTCCATCT TAGGGCCCCA CCACTTGGAT 
GAGTTTGTCC GCGTCTGGGC AGAGTACGAC CGAGCTGCAT GTGGCCGCAT CCATTATACT 
GAGATGTATG AAATGCTGAC TCTCATGTCA CCACCGCTAG GCCTCGGCAA GAGATGTCCC 
TCCAAAGTGG CATATAAGAG GTTGGTCCTA ATGAATATGC CAGTAGCTGA GGACATGACA 
GTCCACTTCA CCTCCACACT GATGGCTCTG ATCCGGACAG CCCTGGACAT CAAAATCGCC 
AAAGGTGGTG CAGACAGGCA GCAGCTAGAC TCGGAGCTGC AAAAGGAGAC CCTGGCCATC 
TGGCCTCACC TGTCCCAGAA GATGCTAGAC CTGCTTGTGC CCATGCCCAA AGCCTCTGAC 
CTGACTGTGG GCAAAATCTA TGCAGCAATG ATGATCATGG ACTACTATAA ACAGAGTAAG 
GTGAAGAAAC AGAGGCAGCA GCTGGAGGAA CAGAAAAATG CACCCATGTT CCAGCGAATG 
GAGCCCTCAT CTCTGCCTCA GGAGATCATT GCTAATGCCA AAGCCCTGCC CTATCTCCAG 
CAGGACCCCG TTTCAGGCCT GAGTGGCCGT AGTGGATACC CTTCGATGAG TCCACTCTCC 
CCCCAGGAAA TATTCCAATT GGCTTGTATG GATCCAGCCG ATGATGGACA GTTCCAGGAA 
CATCAGTCTC TGGAGCCAGA GGTTAGTGAA TTAAAAAGCG TGCAGTCCTC TAACCATGGC 
ATCTACCTTC CTTCGGACAC CCAGGAGCAT ACGGGATCTG GGAGGGCATC TTCTATGCCA 
CGCCTGACTG TGGATCCCCA GGTGGTGACA GATACTAGCT CCATGAGACG TTCATTTTCC 
ACTATTCGGG ATAAGCGTTC AAATTCCTCC TGGTTGGAGG AATTCTCCAT GGAGAGAAGC 
AGTGAAAACA CCTACAAGTC ACGTCGTCGG AGTTACCACT CCTCCCTGAG ACTGTCAGCT 
CATCGCCTGA ATTCTGACTC AGGCCACAAG TCTGACACCC ACCGCTCAGG GGGTAGAGAG 
CGGGGACGAT CGAAAGAGCG AAAGCATCTT CTCTCTCCTG ATGTCTCCCG CTGCAACTCA 
GAGGAGCGAG GGACCCAGGC CGACTGGGAG TCCCCAGAGC GCCAACAGTC CAGGTCTCCC 
AGTGAGGCCA GGTCACAGAC CCCCAACAGA CAGGGCACGG GCTCCCTGAG CGAGAGCTCC 
ATCCCCTCCA TCTCTGACAC CAGCACCCCA AGACGAAGTC GCCGGCAGCT CCCACCCGTC 
CCACCAAAGC CTCGGCCCCT CCTCTCCTAC AGCTCCCTGA TTCGACACTC CGGGAGCATC 
TCCCCGCCGG CCGACGGAAG CGAGAGGGGC TCCCCGCTGA CCCCCCAGGC GCTGGAGAGC 
ACGGAGGCCG GCCTGGCGGA GCCCTCGGGC CCCCGGCACG GGCACGGGCA GGGGCACGGG 
CAGGGGCACG GGCAGCAGAG CCAGCACTGC TCCCCGCAGC GCTACATCTC CGAGCCCTAC 
CTGGCCCTGC ACGAAGACTC CCACGCGTCG GACTGTGGCG AGGAGGAGAC GCTCACCTTC 
GAGGCCGCCG TGGCCACCAG CCTGGGCCGC TCCAACACCA TCGGCTCGGC CCCGCCGCTG 
CGGCACAGCT GGCAGATGCC CAACGGGCAC TATCGGCGGC GGCGGCGCGG GGGGCCGGGG 
CCGGGCGTGA TGTGCGGGGC GGTCAGCGAC CTCCTGAGTG ACACGGAAGA AGACGACAAG 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_022420931.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022276639.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe289497
Clone ID Related Accession (Same CDS sequence)	XM_022420931.1
Accession Version	XM_022420931.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGGAC CGAGCCCAGG CCGCAGGCTG CCCGAGCCCG CCCCCTGCCC CGCCGCGGCC 
GGGAGCCCTG AGCCCTGGGC CGCGGGCCGC CGCCGCCACG TCCTGGCCCT GCCTGAGGAG 
GAGGCATGGA GGCCCCAGCA GTGCTCCCTG CTCGAGGCCG ACGCCTCGGA CGAGGTGGGC 
ATGCTGCCGG CCTCCCCGCG CGCCGCCGCC TCCGGCGTCG ACAACTTCTT CCCGGTGCAG 
GAGGGCGAGG GCGCGGGCTG GGAGGGCGGT GCGGCCCTGG ACGCCGGCGC CGGCCCCTTC 
CTCCCGGTGA GCCCCGAGGT GATGAAGCGG CGGCGCGGGG GCCTCATCGA GCAGCGCGAC 
ATCATCAAGG CCCACGAGGC GCACAAGATG CAGAGCACCC CGCAGGCGCG GCGCAAGGAG 
TGGGAGATGG CTCGCTTCGG GGAGGCGGTG GTCGGCAGGC CGGGGTCGGG CGATGGAGAC 
TCGGACCAGA GCAGGAACCG GCAAGGAACC CCGGTGCCGG CCTCGGGGCC GGCGGCCGCC 
TACAAGCAGT CGAAAGCGCA GAGGGCGCGG ACTATGGCTT TGTACAACCC CATTCCCGTC 
CGGCAGAACT GTTTCACCGT CAACAGATCC CTGTTCATCT TCGGAGAAGA TAACATTGTC 
AGGAAATACG CCAAGAAGCT CATCGACTGG CCGCCGTTTG AGTACATGAT TTTGGCAACC 
ATCATTGCCA ACTGCATTGT CTTGGCCCTG GAACAGCATC TTCCTGAGGA TGACAAGACC 
CCGATGTCCC GCAGACTGGA GAAGACAGAG CCGTATTTCA TTGGGATCTT TTGCTTTGAA 
GCCGGTATCA AAATTGTGGC CCTGGGGTTC ATCTTTCATA AGGGCTCGTA CCTCCGCAAT 
GGCTGGAATG TCATGGACTT CATCGTGGTC CTCAGTGGCA TCCTGGCCAC TGCGGGGACT 
CACTTCAACA CACACGTGGA CCTGAGGACC CTCCGGGCTG TGCGTGTCCT GAGACCCCTG 
AAGCTCGTGT CAGGGATTCC TAGCCTGCAG ATCGTGTTGA AGTCTATCAT GAAGGCCATG 
GTGCCTCTTC TGCAAATTGG TCTCCTGCTC TTCTTTGCCA TCTTGATGTT TGCTATCATT 
GGCTTAGAGT TCTACAGTGG GAAGTTGCAC CGAGCATGTT TCACGAACAA TTCAGGTATT 
CTGGAAGGAT TTGACCCCCC TCACCCATGT GGTGTGCAGG GCTGCCCAGC TGGCTACGAA 
TGCCGGGACT GGATTGGCCC CAATGATGGG ATCACCCAGT TTGATAACAT CCTTTTTGCT 
GTGCTGACTG TCTTCCAATG CATCACCATG GAAGGATGGA CCACCGTGCT GTACAATACC 
AATGATGCCT TAGGAGCCAC CTGGAACTGG CTGTACTTCA TCCCCCTCAT CATCATTGGA 
TCCTTCTTTG TTCTCAACCT AGTTCTGGGA GTGCTTTCCG GGGAGTTTGC CAAAGAGAGA 
GAGAGAGTGG AGAACCGGAG AGCTTTCATG AAACTGCGGC GGCAGCAGCA GATTGAGCGG 
GAGCTGAACG GGTACCGCGC CTGGATAGAC AAAGCAGAGG AAGTCATGCT TGCTGAAGAA 
AATAAAAATG CTGGGACGTC AGCCTTAGAA GTGCTTCGAA GGGCAACCAT CAAAAGGAGC 
CGGACAGAGG CCATGACTCG AGACTCCAGT GATGAACACT GTGTCGATAT CTCCTCTGTG 
GGCACACCTC TCGCCCGAGC CAGCATCAAA AGTGCAAAGG TGGATGGGGC CTCCTATTTC 
CGGCACAAAG AAAGGCTTCT GCGCATCTCT ATCCGCCACA TGGTGAAATC CCAGGTGTTT 
TACTGGATTG TGTTGAGCCT TGTGGCTCTC AACACTGCCT GTGTGGCCAT CGTCCATCAT 
AACCAGCCCC AGTGGCTCAC CCATCTCCTC TACTATGCAG AATTTTTGTT TCTGGGACTG 
TTCCTCTTGG AGATGTCCCT GAAGATGTAT GGCATGGGAC CTCGACTTTA TTTTCACTCT 
TCATTCAACT GTTTTGATTT TGGGGTCACA GTGGGCAGCA TCTTTGAAGT GGTCTGGGCA 
ATCTTCAGAC CTGGCACATC TTTTGGAATC AGTGTCTTAC GGGCTCTCCG GCTTCTAAGA 
ATATTTAAAA TAACCAAGTA TTGGGCATCT CTGCGAAATT TGGTGGTCTC CTTGATGAGC 
TCTATGAAGT CTATCATCAG TTTGCTCTTC CTCCTCTTCC TCTTCATCGT TGTCTTTGCT 
CTCCTAGGAA TGCAGCTGTT TGGAGGCAGG TTTAACTTTA ATGATGGGAC TCCTTCAGCA 
AACTTTGATA CCTTCCCTGC AGCCATCATG ACTGTATTCC AGATCCTAAC AGGTGAGGAC 
TGGAATGAGG TGATGTACAA TGGGATCCGT TCCCAGGGTG GAGTTAGCTC AGGCATGTGG 
TCAGCTATCT ATTTCATTGT GCTCACCTTG TTTGGAAACT ACACACTGCT GAATGTGTTC 
TTGGCCATTG CTGTGGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA TGAACAAGAA 
GAAGAGGAGG CTTTTAACCA GAAACATGCA TTGCAGAAGG CCAAGGAGGT CAGCCCGATG 
TCTGCACCCA ACATGCCTTC TATCGAGAGG GAGCGGAGGC GCCGGCACCA CATGTCGGTG 
TGGGAGCAGC GCACCAGCCA GCTGAGAAGG CACATGCAGA TGTCCAGCCA GGAGGCCCTC 
AATAAAGAGG AGGCCCCACC GATGAACCCC CTCAACCCCC TCAACCCGCT GAGCCCCCTC 
AACCCGCTCA ACGCCCACCC CAGCCTCTAC CGGAGACCTA GGCCTATTGA GGGCCTGGGC 
CTGGGCCTGG AGAAGTGTGA GGAGGAGCGC ATCATCAGCC GCGGGGGGTC CCTCAAGGGG 
GACGGAGGGG ACCTGTCGAG TGCCCTGGAC AACCAGAGGA GCCCTTTGTC CCTGGGCAAA 
CGGGAGCCAC CATGGCTGGC CAGGCCCTGT CATGGGAACT GTGATCCGAC CCAGCAGGAG 
GCTGGGGGAG GGGAGACCGT GGTGACCTTC GAGGACCGGG CCAGGCACAG GCAGAGCCAG 
CGGCGCAGCC GGCACCGTCG TGTCAGGACG GAAGGCAAGG AGCTCTCCTC CACCTCCCGG 
AGCAGGTCTG TCAGCCAGGA GCGGAGTCTG GATGAGGGGG TGCTCACCGA CGGGGAGAAG 
GAGCGAGAGC CCAGGGGCGG CCATGCAGGC AAAGAACCCA CCATCCAAGA AGAAGACAGA 
GGCCAAGACT TAAGGAGGAC GAACAGTCTG ATGGTGCCCA GAGGCTCTGG GCTGGCAGGA 
GCCCTTGATG AGGCCAACAC ACCCCTAGTC CTGCCCCACC ACGATCTGGA AGTGGGGAAG 
GACACGGTGC TGACGGAGCA GGAGCCGGAG GGCAGCAGCG AGCAGGCCCT GCTGGGGGAT 
GTGCAGTTGG ACATTGGCAG GGTCATCAGC CAGAGTGAGC CTGACCTCTC CTGCGTCACA 
GCCAACACAG ACAAGGCCAC CACCGACAGC ACCAGCGTCA CCGTCGCCAT CCCTGATGTG 
GGCCCCTTGA TGGACTCAAC TGTGATTCAC ATTAGCAACA AAACTGATGG AGAAGCCAGT 
CCCTTGAAGG AGGCTGAGAT CAAAGAGGAG GAGGAGGAAA TGGAGAAGAA GCAGCAGAAG 
AAGGAGAAGC GTGAGACGGG CAAAGCCATG GTGCCCCACA GCTCTATGTT CATCTTCAGC 
ACCACCAACC CGATTCGCAG GGCCTGCCAC TACATCGTGA ACCTGCGCTA CTTTGAGATG 
TGCATTCTCC TGGTGATCGC CGCCAGCAGC ATTGCCCTGG CAGCAGAGGA CCCCGTCCTG 
ACCAACTCGG AGCGCAACAA AGTCCTGAGG TATTTTGACT ATGTTTTCAC TGGCGTGTTC 
ACCTTTGAGA TGGTTATAAA GATGATAGAC CAGGGCTTGA TCCTACAGGA TGGGTCCTAC 
TTCCGAGACC TGTGGAACAT CCTGGACTTT GTGGTGGTGG TTGGTGCATT GGTGGCCTTT 
GCTTTGGCGA ACGCTTTGGG AACAAACAAG GGGCGAGACA TCAAGACCAT CAAATCCCTG 
AGGGTGCTCC GAGTTCTGAG GCCACTGAAG ACCATCAAGC GCTTGCCCAA ACTCAAGGCT 
GTCTTTGACT GTGTGGTGAC CTCCTTGAAG AATGTCTTCA ACATACTCAT CGTCTATAAG 
CTCTTCATGT TCATCTTTGC TGTCATTGCG GTTCAGCTCT TCAAGGGAAA ATTCTTTTAT 
TGCACAGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTACGT AGATCATGAA 
AAAAACAAGA TGGAGGTGAA AGGCCGGGAA TGGAAGCGCC ATGAATTCCA CTACGACAAC 
ATTATCTGGG CCCTGCTGAC CCTCTTCACC GTCTCCACGG GGGAAGGATG GCCTCAAGTC 
TTACAGCATT CTGTCGATGT GACAGAGGAA GACCGAGGAC CAAGCCGCAG CAACCGCATG 
GAGATGTCCA TCTTCTATGT GGTCTACTTT GTGGTCTTCC CTTTCTTCTT TGTCAATATC 
TTTGTGGCTC TGATCATCAT CACCTTCCAG GAACAAGGGG ACAAGATGAT GGAGGAATGC 
AGCCTGGAGA AGAACGAGAG GGCGTGCATT GACTTCGCCA TCAGTGCCAA ACCTCTCACC 
CGCTACATGC CACAGAACAG GCACACCTTC CAGTACCGCG TCTGGCACTT CGTGGTGTCT 
CCGTCCTTTG AGTACACCAT CATGGCCATG ATCGCCCTGA ACACCGTCGT GCTGATGATG 
AAGTACTACT CTGCTCCCTG TACCTATGAA CTGGCCCTGA AGTACCTGAA TATTGCCTTC 
ACCATGGTGT TTTCCCTGGA ATGTGTCCTA AAGATCATCG CTTTTGGCTT CTTGAACTAC 
TTTCGAGACA CCTGGAACAT CTTTGACTTC ATCACTGTGA TTGGCAGTAT CACAGAAATC 
ATACTGACAG ACAGCAAGCT GGTAAACACC AGTGGTTTCA ACATGAGCTT TCTGAAGCTC 
TTCCGTGCCG CCCGCCTCAT CAAGCTTCTG CGGCAGGGCT ACACCATCCG TATTTTACTC 
TGGACCTTCG TGCAGTCCTT TAAGGCCCTC CCCTACGTCT GCCTTTTGAT TGCCATGCTT 
TTCTTCATCT ATGCCATCAT TGGGATGCAG GTATTTGGAA ACATAAAATT AGATGAGGAG 
AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GGTCCCTCAT GTTACTCTTC 
AGGAGTGCCA CGGGAGAGGC CTGGCAGGAG ATAATGCTGT CGTGCCTCGG GGAGAAAGGC 
TGCGAGCCCG ACACCACTGC GCCATCGGGG CAGAACGAGA ATGAGCGCTG TGGCACCGAT 
CTGGCCTATG TTTACTTTGT CTCCTTCATC TTCTTCTGCT CTTTCTTGAT GCTCAACTTG 
TTTGTGGCCG TCATCATGGA CAACTTCGAG TACCTGACTC GGGACTCCTC CATCTTAGGG 
CCCCACCACT TGGATGAGTT TGTCCGCGTC TGGGCAGAGT ACGACCGAGC TGCATGTGGC 
CGCATCCATT ATACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCACC GCTAGGCCTC 
GGCAAGAGAT GTCCCTCCAA AGTGGCATAT AAGAGGTTGG TCCTAATGAA TATGCCAGTA 
GCTGAGGACA TGACAGTCCA CTTCACCTCC ACACTGATGG CTCTGATCCG GACAGCCCTG 
GACATCAAAA TCGCCAAAGG TGGTGCAGAC AGGCAGCAGC TAGACTCGGA GCTGCAAAAG 
GAGACCCTGG CCATCTGGCC TCACCTGTCC CAGAAGATGC TAGACCTGCT TGTGCCCATG 
CCCAAAGCCT CTGACCTGAC TGTGGGCAAA ATCTATGCAG CAATGATGAT CATGGACTAC 
TATAAACAGA GTAAGGTGAA GAAACAGAGG CAGCAGCTGG AGGAACAGAA AAATGCACCC 
ATGTTCCAGC GAATGGAGCC CTCATCTCTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC 
CTGCCCTATC TCCAGCAGGA CCCCGTTTCA GGCCTGAGTG GCCGTAGTGG ATACCCTTCG 
ATGAGTCCAC TCTCCCCCCA GGAAATATTC CAATTGGCTT GTATGGATCC AGCCGATGAT 
GGACAGTTCC AGGAACATCA GTCTCTGGAG CCAGAGGTTA GTGAATTAAA AAGCGTGCAG 
TCCTCTAACC ATGGCATCTA CCTTCCTTCG GACACCCAGG AGCATACGGG ATCTGGGAGG 
GCATCTTCTA TGCCACGCCT GACTGTGGAT CCCCAGGTGG TGACAGATAC TAGCTCCATG 
AGACGTTCAT TTTCCACTAT TCGGGATAAG CGTTCAAATT CCTCCTGGTT GGAGGAATTC 
TCCATGGAGA GAAGCAGTGA AAACACCTAC AAGTCACGTC GTCGGAGTTA CCACTCCTCC 
CTGAGACTGT CAGCTCATCG CCTGAATTCT GACTCAGGCC ACAAGTCTGA CACCCACCGC 
TCAGGGGGTA GAGAGCGGGG ACGATCGAAA GAGCGAAAGC ATCTTCTCTC TCCTGATGTC 
TCCCGCTGCA ACTCAGAGGA GCGAGGGACC CAGGCCGACT GGGAGTCCCC AGAGCGCCAA 
CAGTCCAGGT CTCCCAGTGA GGCCAGGTCA CAGACCCCCA ACAGACAGGG CACGGGCTCC 
CTGAGCGAGA GCTCCATCCC CTCCATCTCT GACACCAGCA CCCCAAGACG AAGTCGCCGG 
CAGCTCCCAC CCGTCCCACC AAAGCCTCGG CCCCTCCTCT CCTACAGCTC CCTGATTCGA 
CACTCCGGGA GCATCTCCCC GCCGGCCGAC GGAAGCGAGA GGGGCTCCCC GCTGACCCCC 
CAGGCGCTGG AGAGCACGGA GGCCGGCCTG GCGGAGCCCT CGGGCCCCCG GCACGGGCAC 
GGGCAGGGGC ACGGGCAGGG GCACGGGCAG CAGAGCCAGC ACTGCTCCCC GCAGCGCTAC 
ATCTCCGAGC CCTACCTGGC CCTGCACGAA GACTCCCACG CGTCGGACTG TGGCGAGGAG 
GAGACGCTCA CCTTCGAGGC CGCCGTGGCC ACCAGCCTGG GCCGCTCCAA CACCATCGGC 
TCGGCCCCGC CGCTGCGGCA CAGCTGGCAG ATGCCCAACG GGCACTATCG GCGGCGGCGG 
CGCGGGGGGC CGGGGCCGGG CGTGATGTGC GGGGCGGTCA GCGACCTCCT GAGTGACACG 
GAAGAAGACG ACAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276639.1
CDS	4..7344
Translation	MRGPSPGRRLPEPAPCPAAAGSPEPWAAGRRRHVLALPEEEAWRPQQCSLLEADASDEVGMLPASPRAAASGVDNFFPVQEGEGAGWEGGAALDAGAGPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFTNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLGLGLEKCEEERIISRGGSLKGDGGDLSSALDNQRSPLSLGKREPPWLARPCHGNCDPTQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKELSSTSRSRSVSQERSLDEGVLTDGEKEREPRGGHAGKEPTIQEEDRGQDLRRTNSLMVPRGSGLAGALDEANTPLVLPHHDLEVGKDTVLTEQEPEGSSEQALLGDVQLDIGRVISQSEPDLSCVTANTDKATTDSTSVTVAIPDVGPLMDSTVIHISNKTDGEASPLKEAEIKEEEEEMEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEHQSLEPEVSELKSVQSSNHGIYLPSDTQEHTGSGRASSMPRLTVDPQVVTDTSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERQQSRSPSEARSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHSGSISPPADGSERGSPLTPQALESTEAGLAEPSGPRHGHGQGHGQGHGQQSQHCSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGVMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_022420931.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022420931.1

ORF Insert Sequence:

ATGCGTGGAC CGAGCCCAGG CCGCAGGCTG CCCGAGCCCG CCCCCTGCCC CGCCGCGGCC 
GGGAGCCCTG AGCCCTGGGC CGCGGGCCGC CGCCGCCACG TCCTGGCCCT GCCTGAGGAG 
GAGGCATGGA GGCCCCAGCA GTGCTCCCTG CTCGAGGCCG ACGCCTCGGA CGAGGTGGGC 
ATGCTGCCGG CCTCCCCGCG CGCCGCCGCC TCCGGCGTCG ACAACTTCTT CCCGGTGCAG 
GAGGGCGAGG GCGCGGGCTG GGAGGGCGGT GCGGCCCTGG ACGCCGGCGC CGGCCCCTTC 
CTCCCGGTGA GCCCCGAGGT GATGAAGCGG CGGCGCGGGG GCCTCATCGA GCAGCGCGAC 
ATCATCAAGG CCCACGAGGC GCACAAGATG CAGAGCACCC CGCAGGCGCG GCGCAAGGAG 
TGGGAGATGG CTCGCTTCGG GGAGGCGGTG GTCGGCAGGC CGGGGTCGGG CGATGGAGAC 
TCGGACCAGA GCAGGAACCG GCAAGGAACC CCGGTGCCGG CCTCGGGGCC GGCGGCCGCC 
TACAAGCAGT CGAAAGCGCA GAGGGCGCGG ACTATGGCTT TGTACAACCC CATTCCCGTC 
CGGCAGAACT GTTTCACCGT CAACAGATCC CTGTTCATCT TCGGAGAAGA TAACATTGTC 
AGGAAATACG CCAAGAAGCT CATCGACTGG CCGCCGTTTG AGTACATGAT TTTGGCAACC 
ATCATTGCCA ACTGCATTGT CTTGGCCCTG GAACAGCATC TTCCTGAGGA TGACAAGACC 
CCGATGTCCC GCAGACTGGA GAAGACAGAG CCGTATTTCA TTGGGATCTT TTGCTTTGAA 
GCCGGTATCA AAATTGTGGC CCTGGGGTTC ATCTTTCATA AGGGCTCGTA CCTCCGCAAT 
GGCTGGAATG TCATGGACTT CATCGTGGTC CTCAGTGGCA TCCTGGCCAC TGCGGGGACT 
CACTTCAACA CACACGTGGA CCTGAGGACC CTCCGGGCTG TGCGTGTCCT GAGACCCCTG 
AAGCTCGTGT CAGGGATTCC TAGCCTGCAG ATCGTGTTGA AGTCTATCAT GAAGGCCATG 
GTGCCTCTTC TGCAAATTGG TCTCCTGCTC TTCTTTGCCA TCTTGATGTT TGCTATCATT 
GGCTTAGAGT TCTACAGTGG GAAGTTGCAC CGAGCATGTT TCACGAACAA TTCAGGTATT 
CTGGAAGGAT TTGACCCCCC TCACCCATGT GGTGTGCAGG GCTGCCCAGC TGGCTACGAA 
TGCCGGGACT GGATTGGCCC CAATGATGGG ATCACCCAGT TTGATAACAT CCTTTTTGCT 
GTGCTGACTG TCTTCCAATG CATCACCATG GAAGGATGGA CCACCGTGCT GTACAATACC 
AATGATGCCT TAGGAGCCAC CTGGAACTGG CTGTACTTCA TCCCCCTCAT CATCATTGGA 
TCCTTCTTTG TTCTCAACCT AGTTCTGGGA GTGCTTTCCG GGGAGTTTGC CAAAGAGAGA 
GAGAGAGTGG AGAACCGGAG AGCTTTCATG AAACTGCGGC GGCAGCAGCA GATTGAGCGG 
GAGCTGAACG GGTACCGCGC CTGGATAGAC AAAGCAGAGG AAGTCATGCT TGCTGAAGAA 
AATAAAAATG CTGGGACGTC AGCCTTAGAA GTGCTTCGAA GGGCAACCAT CAAAAGGAGC 
CGGACAGAGG CCATGACTCG AGACTCCAGT GATGAACACT GTGTCGATAT CTCCTCTGTG 
GGCACACCTC TCGCCCGAGC CAGCATCAAA AGTGCAAAGG TGGATGGGGC CTCCTATTTC 
CGGCACAAAG AAAGGCTTCT GCGCATCTCT ATCCGCCACA TGGTGAAATC CCAGGTGTTT 
TACTGGATTG TGTTGAGCCT TGTGGCTCTC AACACTGCCT GTGTGGCCAT CGTCCATCAT 
AACCAGCCCC AGTGGCTCAC CCATCTCCTC TACTATGCAG AATTTTTGTT TCTGGGACTG 
TTCCTCTTGG AGATGTCCCT GAAGATGTAT GGCATGGGAC CTCGACTTTA TTTTCACTCT 
TCATTCAACT GTTTTGATTT TGGGGTCACA GTGGGCAGCA TCTTTGAAGT GGTCTGGGCA 
ATCTTCAGAC CTGGCACATC TTTTGGAATC AGTGTCTTAC GGGCTCTCCG GCTTCTAAGA 
ATATTTAAAA TAACCAAGTA TTGGGCATCT CTGCGAAATT TGGTGGTCTC CTTGATGAGC 
TCTATGAAGT CTATCATCAG TTTGCTCTTC CTCCTCTTCC TCTTCATCGT TGTCTTTGCT 
CTCCTAGGAA TGCAGCTGTT TGGAGGCAGG TTTAACTTTA ATGATGGGAC TCCTTCAGCA 
AACTTTGATA CCTTCCCTGC AGCCATCATG ACTGTATTCC AGATCCTAAC AGGTGAGGAC 
TGGAATGAGG TGATGTACAA TGGGATCCGT TCCCAGGGTG GAGTTAGCTC AGGCATGTGG 
TCAGCTATCT ATTTCATTGT GCTCACCTTG TTTGGAAACT ACACACTGCT GAATGTGTTC 
TTGGCCATTG CTGTGGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA TGAACAAGAA 
GAAGAGGAGG CTTTTAACCA GAAACATGCA TTGCAGAAGG CCAAGGAGGT CAGCCCGATG 
TCTGCACCCA ACATGCCTTC TATCGAGAGG GAGCGGAGGC GCCGGCACCA CATGTCGGTG 
TGGGAGCAGC GCACCAGCCA GCTGAGAAGG CACATGCAGA TGTCCAGCCA GGAGGCCCTC 
AATAAAGAGG AGGCCCCACC GATGAACCCC CTCAACCCCC TCAACCCGCT GAGCCCCCTC 
AACCCGCTCA ACGCCCACCC CAGCCTCTAC CGGAGACCTA GGCCTATTGA GGGCCTGGGC 
CTGGGCCTGG AGAAGTGTGA GGAGGAGCGC ATCATCAGCC GCGGGGGGTC CCTCAAGGGG 
GACGGAGGGG ACCTGTCGAG TGCCCTGGAC AACCAGAGGA GCCCTTTGTC CCTGGGCAAA 
CGGGAGCCAC CATGGCTGGC CAGGCCCTGT CATGGGAACT GTGATCCGAC CCAGCAGGAG 
GCTGGGGGAG GGGAGACCGT GGTGACCTTC GAGGACCGGG CCAGGCACAG GCAGAGCCAG 
CGGCGCAGCC GGCACCGTCG TGTCAGGACG GAAGGCAAGG AGCTCTCCTC CACCTCCCGG 
AGCAGGTCTG TCAGCCAGGA GCGGAGTCTG GATGAGGGGG TGCTCACCGA CGGGGAGAAG 
GAGCGAGAGC CCAGGGGCGG CCATGCAGGC AAAGAACCCA CCATCCAAGA AGAAGACAGA 
GGCCAAGACT TAAGGAGGAC GAACAGTCTG ATGGTGCCCA GAGGCTCTGG GCTGGCAGGA 
GCCCTTGATG AGGCCAACAC ACCCCTAGTC CTGCCCCACC ACGATCTGGA AGTGGGGAAG 
GACACGGTGC TGACGGAGCA GGAGCCGGAG GGCAGCAGCG AGCAGGCCCT GCTGGGGGAT 
GTGCAGTTGG ACATTGGCAG GGTCATCAGC CAGAGTGAGC CTGACCTCTC CTGCGTCACA 
GCCAACACAG ACAAGGCCAC CACCGACAGC ACCAGCGTCA CCGTCGCCAT CCCTGATGTG 
GGCCCCTTGA TGGACTCAAC TGTGATTCAC ATTAGCAACA AAACTGATGG AGAAGCCAGT 
CCCTTGAAGG AGGCTGAGAT CAAAGAGGAG GAGGAGGAAA TGGAGAAGAA GCAGCAGAAG 
AAGGAGAAGC GTGAGACGGG CAAAGCCATG GTGCCCCACA GCTCTATGTT CATCTTCAGC 
ACCACCAACC CGATTCGCAG GGCCTGCCAC TACATCGTGA ACCTGCGCTA CTTTGAGATG 
TGCATTCTCC TGGTGATCGC CGCCAGCAGC ATTGCCCTGG CAGCAGAGGA CCCCGTCCTG 
ACCAACTCGG AGCGCAACAA AGTCCTGAGG TATTTTGACT ATGTTTTCAC TGGCGTGTTC 
ACCTTTGAGA TGGTTATAAA GATGATAGAC CAGGGCTTGA TCCTACAGGA TGGGTCCTAC 
TTCCGAGACC TGTGGAACAT CCTGGACTTT GTGGTGGTGG TTGGTGCATT GGTGGCCTTT 
GCTTTGGCGA ACGCTTTGGG AACAAACAAG GGGCGAGACA TCAAGACCAT CAAATCCCTG 
AGGGTGCTCC GAGTTCTGAG GCCACTGAAG ACCATCAAGC GCTTGCCCAA ACTCAAGGCT 
GTCTTTGACT GTGTGGTGAC CTCCTTGAAG AATGTCTTCA ACATACTCAT CGTCTATAAG 
CTCTTCATGT TCATCTTTGC TGTCATTGCG GTTCAGCTCT TCAAGGGAAA ATTCTTTTAT 
TGCACAGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTACGT AGATCATGAA 
AAAAACAAGA TGGAGGTGAA AGGCCGGGAA TGGAAGCGCC ATGAATTCCA CTACGACAAC 
ATTATCTGGG CCCTGCTGAC CCTCTTCACC GTCTCCACGG GGGAAGGATG GCCTCAAGTC 
TTACAGCATT CTGTCGATGT GACAGAGGAA GACCGAGGAC CAAGCCGCAG CAACCGCATG 
GAGATGTCCA TCTTCTATGT GGTCTACTTT GTGGTCTTCC CTTTCTTCTT TGTCAATATC 
TTTGTGGCTC TGATCATCAT CACCTTCCAG GAACAAGGGG ACAAGATGAT GGAGGAATGC 
AGCCTGGAGA AGAACGAGAG GGCGTGCATT GACTTCGCCA TCAGTGCCAA ACCTCTCACC 
CGCTACATGC CACAGAACAG GCACACCTTC CAGTACCGCG TCTGGCACTT CGTGGTGTCT 
CCGTCCTTTG AGTACACCAT CATGGCCATG ATCGCCCTGA ACACCGTCGT GCTGATGATG 
AAGTACTACT CTGCTCCCTG TACCTATGAA CTGGCCCTGA AGTACCTGAA TATTGCCTTC 
ACCATGGTGT TTTCCCTGGA ATGTGTCCTA AAGATCATCG CTTTTGGCTT CTTGAACTAC 
TTTCGAGACA CCTGGAACAT CTTTGACTTC ATCACTGTGA TTGGCAGTAT CACAGAAATC 
ATACTGACAG ACAGCAAGCT GGTAAACACC AGTGGTTTCA ACATGAGCTT TCTGAAGCTC 
TTCCGTGCCG CCCGCCTCAT CAAGCTTCTG CGGCAGGGCT ACACCATCCG TATTTTACTC 
TGGACCTTCG TGCAGTCCTT TAAGGCCCTC CCCTACGTCT GCCTTTTGAT TGCCATGCTT 
TTCTTCATCT ATGCCATCAT TGGGATGCAG GTATTTGGAA ACATAAAATT AGATGAGGAG 
AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GGTCCCTCAT GTTACTCTTC 
AGGAGTGCCA CGGGAGAGGC CTGGCAGGAG ATAATGCTGT CGTGCCTCGG GGAGAAAGGC 
TGCGAGCCCG ACACCACTGC GCCATCGGGG CAGAACGAGA ATGAGCGCTG TGGCACCGAT 
CTGGCCTATG TTTACTTTGT CTCCTTCATC TTCTTCTGCT CTTTCTTGAT GCTCAACTTG 
TTTGTGGCCG TCATCATGGA CAACTTCGAG TACCTGACTC GGGACTCCTC CATCTTAGGG 
CCCCACCACT TGGATGAGTT TGTCCGCGTC TGGGCAGAGT ACGACCGAGC TGCATGTGGC 
CGCATCCATT ATACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCACC GCTAGGCCTC 
GGCAAGAGAT GTCCCTCCAA AGTGGCATAT AAGAGGTTGG TCCTAATGAA TATGCCAGTA 
GCTGAGGACA TGACAGTCCA CTTCACCTCC ACACTGATGG CTCTGATCCG GACAGCCCTG 
GACATCAAAA TCGCCAAAGG TGGTGCAGAC AGGCAGCAGC TAGACTCGGA GCTGCAAAAG 
GAGACCCTGG CCATCTGGCC TCACCTGTCC CAGAAGATGC TAGACCTGCT TGTGCCCATG 
CCCAAAGCCT CTGACCTGAC TGTGGGCAAA ATCTATGCAG CAATGATGAT CATGGACTAC 
TATAAACAGA GTAAGGTGAA GAAACAGAGG CAGCAGCTGG AGGAACAGAA AAATGCACCC 
ATGTTCCAGC GAATGGAGCC CTCATCTCTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC 
CTGCCCTATC TCCAGCAGGA CCCCGTTTCA GGCCTGAGTG GCCGTAGTGG ATACCCTTCG 
ATGAGTCCAC TCTCCCCCCA GGAAATATTC CAATTGGCTT GTATGGATCC AGCCGATGAT 
GGACAGTTCC AGGAACATCA GTCTCTGGAG CCAGAGGTTA GTGAATTAAA AAGCGTGCAG 
TCCTCTAACC ATGGCATCTA CCTTCCTTCG GACACCCAGG AGCATACGGG ATCTGGGAGG 
GCATCTTCTA TGCCACGCCT GACTGTGGAT CCCCAGGTGG TGACAGATAC TAGCTCCATG 
AGACGTTCAT TTTCCACTAT TCGGGATAAG CGTTCAAATT CCTCCTGGTT GGAGGAATTC 
TCCATGGAGA GAAGCAGTGA AAACACCTAC AAGTCACGTC GTCGGAGTTA CCACTCCTCC 
CTGAGACTGT CAGCTCATCG CCTGAATTCT GACTCAGGCC ACAAGTCTGA CACCCACCGC 
TCAGGGGGTA GAGAGCGGGG ACGATCGAAA GAGCGAAAGC ATCTTCTCTC TCCTGATGTC 
TCCCGCTGCA ACTCAGAGGA GCGAGGGACC CAGGCCGACT GGGAGTCCCC AGAGCGCCAA 
CAGTCCAGGT CTCCCAGTGA GGCCAGGTCA CAGACCCCCA ACAGACAGGG CACGGGCTCC 
CTGAGCGAGA GCTCCATCCC CTCCATCTCT GACACCAGCA CCCCAAGACG AAGTCGCCGG 
CAGCTCCCAC CCGTCCCACC AAAGCCTCGG CCCCTCCTCT CCTACAGCTC CCTGATTCGA 
CACTCCGGGA GCATCTCCCC GCCGGCCGAC GGAAGCGAGA GGGGCTCCCC GCTGACCCCC 
CAGGCGCTGG AGAGCACGGA GGCCGGCCTG GCGGAGCCCT CGGGCCCCCG GCACGGGCAC 
GGGCAGGGGC ACGGGCAGGG GCACGGGCAG CAGAGCCAGC ACTGCTCCCC GCAGCGCTAC 
ATCTCCGAGC CCTACCTGGC CCTGCACGAA GACTCCCACG CGTCGGACTG TGGCGAGGAG 
GAGACGCTCA CCTTCGAGGC CGCCGTGGCC ACCAGCCTGG GCCGCTCCAA CACCATCGGC 
TCGGCCCCGC CGCTGCGGCA CAGCTGGCAG ATGCCCAACG GGCACTATCG GCGGCGGCGG 
CGCGGGGGGC CGGGGCCGGG CGTGATGTGC GGGGCGGTCA GCGACCTCCT GAGTGACACG 
GAAGAAGACG ACAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_022420932.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022276640.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe289498
Clone ID Related Accession (Same CDS sequence)	XM_022420932.1
Accession Version	XM_022420932.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGGAC CGAGCCCAGG CCGCAGGCTG CCCGAGCCCG CCCCCTGCCC CGCCGCGGCC 
GGGAGCCCTG AGCCCTGGGC CGCGGGCCGC CGCCGCCACG TCCTGGCCCT GCCTGAGGAG 
GAGGCATGGA GGCCCCAGCA GTGCTCCCTG CTCGAGGCCG ACGCCTCGGA CGAGGTGGGC 
ATGCTGCCGG CCTCCCCGCG CGCCGCCGCC TCCGGCGTCG ACAACTTCTT CCCGGTGCAG 
GAGGGCGAGG GCGCGGGCTG GGAGGGCGGT GCGGCCCTGG ACGCCGGCGC CGGCCCCTTC 
CTCCCGGTGA GCCCCGAGGT GATGAAGCGG CGGCGCGGGG GCCTCATCGA GCAGCGCGAC 
ATCATCAAGG CCCACGAGGC GCACAAGATG CAGAGCACCC CGCAGGCGCG GCGCAAGGAG 
TGGGAGATGG CTCGCTTCGG GGAGGCGGTG GTCGGCAGGC CGGGGTCGGG CGATGGAGAC 
TCGGACCAGA GCAGGAACCG GCAAGGAACC CCGGTGCCGG CCTCGGGGCC GGCGGCCGCC 
TACAAGCAGT CGAAAGCGCA GAGGGCGCGG ACTATGGCTT TGTACAACCC CATTCCCGTC 
CGGCAGAACT GTTTCACCGT CAACAGATCC CTGTTCATCT TCGGAGAAGA TAACATTGTC 
AGGAAATACG CCAAGAAGCT CATCGACTGG CCGCCGTTTG AGTACATGAT TTTGGCAACC 
ATCATTGCCA ACTGCATTGT CTTGGCCCTG GAACAGCATC TTCCTGAGGA TGACAAGACC 
CCGATGTCCC GCAGACTGGA GAAGACAGAG CCGTATTTCA TTGGGATCTT TTGCTTTGAA 
GCCGGTATCA AAATTGTGGC CCTGGGGTTC ATCTTTCATA AGGGCTCGTA CCTCCGCAAT 
GGCTGGAATG TCATGGACTT CATCGTGGTC CTCAGTGGCA TCCTGGCCAC TGCGGGGACT 
CACTTCAACA CACACGTGGA CCTGAGGACC CTCCGGGCTG TGCGTGTCCT GAGACCCCTG 
AAGCTCGTGT CAGGGATTCC TAGCCTGCAG ATCGTGTTGA AGTCTATCAT GAAGGCCATG 
GTGCCTCTTC TGCAAATTGG TCTCCTGCTC TTCTTTGCCA TCTTGATGTT TGCTATCATT 
GGCTTAGAGT TCTACAGTGG GAAGTTGCAC CGAGCATGTT TCACGAACAA TTCAGGTATT 
CTGGAAGGAT TTGACCCCCC TCACCCATGT GGTGTGCAGG GCTGCCCAGC TGGCTACGAA 
TGCCGGGACT GGATTGGCCC CAATGATGGG ATCACCCAGT TTGATAACAT CCTTTTTGCT 
GTGCTGACTG TCTTCCAATG CATCACCATG GAAGGATGGA CCACCGTGCT GTACAATACC 
AATGATGCCT TAGGAGCCAC CTGGAACTGG CTGTACTTCA TCCCCCTCAT CATCATTGGA 
TCCTTCTTTG TTCTCAACCT AGTTCTGGGA GTGCTTTCCG GGGAGTTTGC CAAAGAGAGA 
GAGAGAGTGG AGAACCGGAG AGCTTTCATG AAACTGCGGC GGCAGCAGCA GATTGAGCGG 
GAGCTGAACG GGTACCGCGC CTGGATAGAC AAAGCAGAGG AAGTCATGCT TGCTGAAGAA 
AATAAAAATG CTGGGACGTC AGCCTTAGAA GTGCTTCGAA GGGCAACCAT CAAAAGGAGC 
CGGACAGAGG CCATGACTCG AGACTCCAGT GATGAACACT GTGTCGATAT CTCCTCTGTG 
GGCACACCTC TCGCCCGAGC CAGCATCAAA AGTGCAAAGG TGGATGGGGC CTCCTATTTC 
CGGCACAAAG AAAGGCTTCT GCGCATCTCT ATCCGCCACA TGGTGAAATC CCAGGTGTTT 
TACTGGATTG TGTTGAGCCT TGTGGCTCTC AACACTGCCT GTGTGGCCAT CGTCCATCAT 
AACCAGCCCC AGTGGCTCAC CCATCTCCTC TACTATGCAG AATTTTTGTT TCTGGGACTG 
TTCCTCTTGG AGATGTCCCT GAAGATGTAT GGCATGGGAC CTCGACTTTA TTTTCACTCT 
TCATTCAACT GTTTTGATTT TGGGGTCACA GTGGGCAGCA TCTTTGAAGT GGTCTGGGCA 
ATCTTCAGAC CTGGCACATC TTTTGGAATC AGTGTCTTAC GGGCTCTCCG GCTTCTAAGA 
ATATTTAAAA TAACCAAGTA TTGGGCATCT CTGCGAAATT TGGTGGTCTC CTTGATGAGC 
TCTATGAAGT CTATCATCAG TTTGCTCTTC CTCCTCTTCC TCTTCATCGT TGTCTTTGCT 
CTCCTAGGAA TGCAGCTGTT TGGAGGCAGG TTTAACTTTA ATGATGGGAC TCCTTCAGCA 
AACTTTGATA CCTTCCCTGC AGCCATCATG ACTGTATTCC AGATCCTAAC AGGTGAGGAC 
TGGAATGAGG TGATGTACAA TGGGATCCGT TCCCAGGGTG GAGTTAGCTC AGGCATGTGG 
TCAGCTATCT ATTTCATTGT GCTCACCTTG TTTGGAAACT ACACACTGCT GAATGTGTTC 
TTGGCCATTG CTGTGGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA TGAACAAGAA 
GAAGAGGAGG CTTTTAACCA GAAACATGCA TTGCAGAAGG CCAAGGAGGT CAGCCCGATG 
TCTGCACCCA ACATGCCTTC TATCGAAAGA GACAGAAGGA GAAGACACCA CATGTCGATG 
TGGGAGCCAC GCAGCAGCCA CCTGAGGGAG CGGAGGCGCC GGCACCACAT GTCGGTGTGG 
GAGCAGCGCA CCAGCCAGCT GAGAAGGCAC ATGCAGATGT CCAGCCAGGA GGCCCTCAAT 
AAAGAGGAGG CCCCACCGAT GAACCCCCTC AACCCCCTCA ACCCGCTGAG CCCCCTCAAC 
CCGCTCAACG CCCACCCCAG CCTCTACCGG AGACCTAGGC CTATTGAGGG CCTGGGCCTG 
GGCCTGGAGA AGTGTGAGGA GGAGCGCATC ATCAGCCGCG GGGGGTCCCT CAAGGGGGAC 
GGAGGGGACC TGTCGAGTGC CCTGGACAAC CAGAGGAGCC CTTTGTCCCT GGGCAAACGG 
GAGCCACCAT GGCTGGCCAG GCCCTGTCAT GGGAACTGTG ATCCGACCCA GCAGGAGGCT 
GGGGGAGGGG AGACCGTGGT GACCTTCGAG GACCGGGCCA GGCACAGGCA GAGCCAGCGG 
CGCAGCCGGC ACCGTCGTGT CAGGACGGAA GGCAAGGAGC TCTCCTCCAC CTCCCGGAGC 
AGGTCTGTCA GCCAGGAGCG GAGTCTGGAT GAGGGGGTGC TCACCGACGG GGAGAAGGAG 
CGAGAGCCCA GGGGCGGCCA TGCAGGCAAA GAACCCACCA TCCAAGAAGA AGACAGAGGC 
CAAGACTTAA GGAGGACGAA CAGTCTGATG GTGCCCAGAG GCTCTGGGCT GGCAGGAGCC 
CTTGATGAGG CCAACACACC CCTAGTCCTG CCCCACCACG ATCTGGAAGT GGGGAAGGAC 
ACGGTGCTGA CGGAGCAGGA GCCGGAGGGC AGCAGCGAGC AGGCCCTGCT GGGGGATGTG 
CAGTTGGACA TTGGCAGGGT CATCAGCCAG AGTGAGCCTG ACCTCTCCTG CGTCACAGCC 
AACACAGACA AGGCCACCAC CGACAGCACC AGCGTCACCG TCGCCATCCC TGATGTGGGC 
CCCTTGATGG ACTCAACTGT GATTCACATT AGCAACAAAA CTGATGGAGA AGCCAGTCCC 
TTGAAGGAGG CTGAGATCAA AGAGGAGGAG GAGGAAATGG AGAAGAAGCA GCAGAAGAAG 
GAGAAGCGTG AGACGGGCAA AGCCATGGTG CCCCACAGCT CTATGTTCAT CTTCAGCACC 
ACCAACCCGA TTCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT TGAGATGTGC 
ATTCTCCTGG TGATCGCCGC CAGCAGCATT GCCCTGGCAG CAGAGGACCC CGTCCTGACC 
AACTCGGAGC GCAACAAAGT CCTGAGGTAT TTTGACTATG TTTTCACTGG CGTGTTCACC 
TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATCC TACAGGATGG GTCCTACTTC 
CGAGACCTGT GGAACATCCT GGACTTTGTG GTGGTGGTTG GTGCATTGGT GGCCTTTGCT 
TTGGCGAACG CTTTGGGAAC AAACAAGGGG CGAGACATCA AGACCATCAA ATCCCTGAGG 
GTGCTCCGAG TTCTGAGGCC ACTGAAGACC ATCAAGCGCT TGCCCAAACT CAAGGCTGTC 
TTTGACTGTG TGGTGACCTC CTTGAAGAAT GTCTTCAACA TACTCATCGT CTATAAGCTC 
TTCATGTTCA TCTTTGCTGT CATTGCGGTT CAGCTCTTCA AGGGAAAATT CTTTTATTGC 
ACAGACAGTT CCAAGGACAC AGAGAAGGAG TGCATAGGCA ACTACGTAGA TCATGAAAAA 
AACAAGATGG AGGTGAAAGG CCGGGAATGG AAGCGCCATG AATTCCACTA CGACAACATT 
ATCTGGGCCC TGCTGACCCT CTTCACCGTC TCCACGGGGG AAGGATGGCC TCAAGTCTTA 
CAGCATTCTG TCGATGTGAC AGAGGAAGAC CGAGGACCAA GCCGCAGCAA CCGCATGGAG 
ATGTCCATCT TCTATGTGGT CTACTTTGTG GTCTTCCCTT TCTTCTTTGT CAATATCTTT 
GTGGCTCTGA TCATCATCAC CTTCCAGGAA CAAGGGGACA AGATGATGGA GGAATGCAGC 
CTGGAGAAGA ACGAGAGGGC GTGCATTGAC TTCGCCATCA GTGCCAAACC TCTCACCCGC 
TACATGCCAC AGAACAGGCA CACCTTCCAG TACCGCGTCT GGCACTTCGT GGTGTCTCCG 
TCCTTTGAGT ACACCATCAT GGCCATGATC GCCCTGAACA CCGTCGTGCT GATGATGAAG 
TACTACTCTG CTCCCTGTAC CTATGAACTG GCCCTGAAGT ACCTGAATAT TGCCTTCACC 
ATGGTGTTTT CCCTGGAATG TGTCCTAAAG ATCATCGCTT TTGGCTTCTT GAACTACTTT 
CGAGACACCT GGAACATCTT TGACTTCATC ACTGTGATTG GCAGTATCAC AGAAATCATA 
CTGACAGACA GCAAGCTGGT AAACACCAGT GGTTTCAACA TGAGCTTTCT GAAGCTCTTC 
CGTGCCGCCC GCCTCATCAA GCTTCTGCGG CAGGGCTACA CCATCCGTAT TTTACTCTGG 
ACCTTCGTGC AGTCCTTTAA GGCCCTCCCC TACGTCTGCC TTTTGATTGC CATGCTTTTC 
TTCATCTATG CCATCATTGG GATGCAGGTA TTTGGAAACA TAAAATTAGA TGAGGAGAGT 
CACATCAACC GGCACAACAA CTTCCGGAGT TTCTTTGGGT CCCTCATGTT ACTCTTCAGG 
AGTGCCACGG GAGAGGCCTG GCAGGAGATA ATGCTGTCGT GCCTCGGGGA GAAAGGCTGC 
GAGCCCGACA CCACTGCGCC ATCGGGGCAG AACGAGAATG AGCGCTGTGG CACCGATCTG 
GCCTATGTTT ACTTTGTCTC CTTCATCTTC TTCTGCTCTT TCTTGATGCT CAACTTGTTT 
GTGGCCGTCA TCATGGACAA CTTCGAGTAC CTGACTCGGG ACTCCTCCAT CTTAGGGCCC 
CACCACTTGG ATGAGTTTGT CCGCGTCTGG GCAGAGTACG ACCGAGCTGC ATGTGGCCGC 
ATCCATTATA CTGAGATGTA TGAAATGCTG ACTCTCATGT CACCACCGCT AGGCCTCGGC 
AAGAGATGTC CCTCCAAAGT GGCATATAAG AGGTTGGTCC TAATGAATAT GCCAGTAGCT 
GAGGACATGA CAGTCCACTT CACCTCCACA CTGATGGCTC TGATCCGGAC AGCCCTGGAC 
ATCAAAATCG CCAAAGGTGG TGCAGACAGG CAGCAGCTAG ACTCGGAGCT GCAAAAGGAG 
ACCCTGGCCA TCTGGCCTCA CCTGTCCCAG AAGATGCTAG ACCTGCTTGT GCCCATGCCC 
AAAGCCTCTG ACCTGACTGT GGGCAAAATC TATGCAGCAA TGATGATCAT GGACTACTAT 
AAACAGAGTA AGGTGAAGAA ACAGAGGCAG CAGCTGGAGG AACAGAAAAA TGCACCCATG 
TTCCAGCGAA TGGAGCCCTC ATCTCTGCCT CAGGAGATCA TTGCTAATGC CAAAGCCCTG 
CCCTATCTCC AGCAGGACCC CGTTTCAGGC CTGAGTGGCC GTAGTGGATA CCCTTCGATG 
AGTCCACTCT CCCCCCAGGA AATATTCCAA TTGGCTTGTA TGGATCCAGC CGATGATGGA 
CAGTTCCAGG AACATCAGTC TCTGGTGGTG ACAGATACTA GCTCCATGAG ACGTTCATTT 
TCCACTATTC GGGATAAGCG TTCAAATTCC TCCTGGTTGG AGGAATTCTC CATGGAGAGA 
AGCAGTGAAA ACACCTACAA GTCACGTCGT CGGAGTTACC ACTCCTCCCT GAGACTGTCA 
GCTCATCGCC TGAATTCTGA CTCAGGCCAC AAGTCTGACA CCCACCGCTC AGGGGGTAGA 
GAGCGGGGAC GATCGAAAGA GCGAAAGCAT CTTCTCTCTC CTGATGTCTC CCGCTGCAAC 
TCAGAGGAGC GAGGGACCCA GGCCGACTGG GAGTCCCCAG AGCGCCAACA GTCCAGGTCT 
CCCAGTGAGG CCAGGTCACA GACCCCCAAC AGACAGGGCA CGGGCTCCCT GAGCGAGAGC 
TCCATCCCCT CCATCTCTGA CACCAGCACC CCAAGACGAA GTCGCCGGCA GCTCCCACCC 
GTCCCACCAA AGCCTCGGCC CCTCCTCTCC TACAGCTCCC TGATTCGACA CTCCGGGAGC 
ATCTCCCCGC CGGCCGACGG AAGCGAGAGG GGCTCCCCGC TGACCCCCCA GGCGCTGGAG 
AGCACGGAGG CCGGCCTGGC GGAGCCCTCG GGCCCCCGGC ACGGGCACGG GCAGGGGCAC 
GGGCAGGGGC ACGGGCAGCA GAGCCAGCAC TGCTCCCCGC AGCGCTACAT CTCCGAGCCC 
TACCTGGCCC TGCACGAAGA CTCCCACGCG TCGGACTGTG GCGAGGAGGA GACGCTCACC 
TTCGAGGCCG CCGTGGCCAC CAGCCTGGGC CGCTCCAACA CCATCGGCTC GGCCCCGCCG 
CTGCGGCACA GCTGGCAGAT GCCCAACGGG CACTATCGGC GGCGGCGGCG CGGGGGGCCG 
GGGCCGGGCG TGATGTGCGG GGCGGTCAGC GACCTCCTGA GTGACACGGA AGAAGACGAC 
AAGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276640.1
CDS	5..7273
Translation	MRGPSPGRRLPEPAPCPAAAGSPEPWAAGRRRHVLALPEEEAWRPQQCSLLEADASDEVGMLPASPRAAASGVDNFFPVQEGEGAGWEGGAALDAGAGPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFTNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLGLGLEKCEEERIISRGGSLKGDGGDLSSALDNQRSPLSLGKREPPWLARPCHGNCDPTQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKELSSTSRSRSVSQERSLDEGVLTDGEKEREPRGGHAGKEPTIQEEDRGQDLRRTNSLMVPRGSGLAGALDEANTPLVLPHHDLEVGKDTVLTEQEPEGSSEQALLGDVQLDIGRVISQSEPDLSCVTANTDKATTDSTSVTVAIPDVGPLMDSTVIHISNKTDGEASPLKEAEIKEEEEEMEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEHQSLVVTDTSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERQQSRSPSEARSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHSGSISPPADGSERGSPLTPQALESTEAGLAEPSGPRHGHGQGHGQGHGQQSQHCSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGVMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_022420932.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022420932.1

ORF Insert Sequence:

ATGCGTGGAC CGAGCCCAGG CCGCAGGCTG CCCGAGCCCG CCCCCTGCCC CGCCGCGGCC 
GGGAGCCCTG AGCCCTGGGC CGCGGGCCGC CGCCGCCACG TCCTGGCCCT GCCTGAGGAG 
GAGGCATGGA GGCCCCAGCA GTGCTCCCTG CTCGAGGCCG ACGCCTCGGA CGAGGTGGGC 
ATGCTGCCGG CCTCCCCGCG CGCCGCCGCC TCCGGCGTCG ACAACTTCTT CCCGGTGCAG 
GAGGGCGAGG GCGCGGGCTG GGAGGGCGGT GCGGCCCTGG ACGCCGGCGC CGGCCCCTTC 
CTCCCGGTGA GCCCCGAGGT GATGAAGCGG CGGCGCGGGG GCCTCATCGA GCAGCGCGAC 
ATCATCAAGG CCCACGAGGC GCACAAGATG CAGAGCACCC CGCAGGCGCG GCGCAAGGAG 
TGGGAGATGG CTCGCTTCGG GGAGGCGGTG GTCGGCAGGC CGGGGTCGGG CGATGGAGAC 
TCGGACCAGA GCAGGAACCG GCAAGGAACC CCGGTGCCGG CCTCGGGGCC GGCGGCCGCC 
TACAAGCAGT CGAAAGCGCA GAGGGCGCGG ACTATGGCTT TGTACAACCC CATTCCCGTC 
CGGCAGAACT GTTTCACCGT CAACAGATCC CTGTTCATCT TCGGAGAAGA TAACATTGTC 
AGGAAATACG CCAAGAAGCT CATCGACTGG CCGCCGTTTG AGTACATGAT TTTGGCAACC 
ATCATTGCCA ACTGCATTGT CTTGGCCCTG GAACAGCATC TTCCTGAGGA TGACAAGACC 
CCGATGTCCC GCAGACTGGA GAAGACAGAG CCGTATTTCA TTGGGATCTT TTGCTTTGAA 
GCCGGTATCA AAATTGTGGC CCTGGGGTTC ATCTTTCATA AGGGCTCGTA CCTCCGCAAT 
GGCTGGAATG TCATGGACTT CATCGTGGTC CTCAGTGGCA TCCTGGCCAC TGCGGGGACT 
CACTTCAACA CACACGTGGA CCTGAGGACC CTCCGGGCTG TGCGTGTCCT GAGACCCCTG 
AAGCTCGTGT CAGGGATTCC TAGCCTGCAG ATCGTGTTGA AGTCTATCAT GAAGGCCATG 
GTGCCTCTTC TGCAAATTGG TCTCCTGCTC TTCTTTGCCA TCTTGATGTT TGCTATCATT 
GGCTTAGAGT TCTACAGTGG GAAGTTGCAC CGAGCATGTT TCACGAACAA TTCAGGTATT 
CTGGAAGGAT TTGACCCCCC TCACCCATGT GGTGTGCAGG GCTGCCCAGC TGGCTACGAA 
TGCCGGGACT GGATTGGCCC CAATGATGGG ATCACCCAGT TTGATAACAT CCTTTTTGCT 
GTGCTGACTG TCTTCCAATG CATCACCATG GAAGGATGGA CCACCGTGCT GTACAATACC 
AATGATGCCT TAGGAGCCAC CTGGAACTGG CTGTACTTCA TCCCCCTCAT CATCATTGGA 
TCCTTCTTTG TTCTCAACCT AGTTCTGGGA GTGCTTTCCG GGGAGTTTGC CAAAGAGAGA 
GAGAGAGTGG AGAACCGGAG AGCTTTCATG AAACTGCGGC GGCAGCAGCA GATTGAGCGG 
GAGCTGAACG GGTACCGCGC CTGGATAGAC AAAGCAGAGG AAGTCATGCT TGCTGAAGAA 
AATAAAAATG CTGGGACGTC AGCCTTAGAA GTGCTTCGAA GGGCAACCAT CAAAAGGAGC 
CGGACAGAGG CCATGACTCG AGACTCCAGT GATGAACACT GTGTCGATAT CTCCTCTGTG 
GGCACACCTC TCGCCCGAGC CAGCATCAAA AGTGCAAAGG TGGATGGGGC CTCCTATTTC 
CGGCACAAAG AAAGGCTTCT GCGCATCTCT ATCCGCCACA TGGTGAAATC CCAGGTGTTT 
TACTGGATTG TGTTGAGCCT TGTGGCTCTC AACACTGCCT GTGTGGCCAT CGTCCATCAT 
AACCAGCCCC AGTGGCTCAC CCATCTCCTC TACTATGCAG AATTTTTGTT TCTGGGACTG 
TTCCTCTTGG AGATGTCCCT GAAGATGTAT GGCATGGGAC CTCGACTTTA TTTTCACTCT 
TCATTCAACT GTTTTGATTT TGGGGTCACA GTGGGCAGCA TCTTTGAAGT GGTCTGGGCA 
ATCTTCAGAC CTGGCACATC TTTTGGAATC AGTGTCTTAC GGGCTCTCCG GCTTCTAAGA 
ATATTTAAAA TAACCAAGTA TTGGGCATCT CTGCGAAATT TGGTGGTCTC CTTGATGAGC 
TCTATGAAGT CTATCATCAG TTTGCTCTTC CTCCTCTTCC TCTTCATCGT TGTCTTTGCT 
CTCCTAGGAA TGCAGCTGTT TGGAGGCAGG TTTAACTTTA ATGATGGGAC TCCTTCAGCA 
AACTTTGATA CCTTCCCTGC AGCCATCATG ACTGTATTCC AGATCCTAAC AGGTGAGGAC 
TGGAATGAGG TGATGTACAA TGGGATCCGT TCCCAGGGTG GAGTTAGCTC AGGCATGTGG 
TCAGCTATCT ATTTCATTGT GCTCACCTTG TTTGGAAACT ACACACTGCT GAATGTGTTC 
TTGGCCATTG CTGTGGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA TGAACAAGAA 
GAAGAGGAGG CTTTTAACCA GAAACATGCA TTGCAGAAGG CCAAGGAGGT CAGCCCGATG 
TCTGCACCCA ACATGCCTTC TATCGAAAGA GACAGAAGGA GAAGACACCA CATGTCGATG 
TGGGAGCCAC GCAGCAGCCA CCTGAGGGAG CGGAGGCGCC GGCACCACAT GTCGGTGTGG 
GAGCAGCGCA CCAGCCAGCT GAGAAGGCAC ATGCAGATGT CCAGCCAGGA GGCCCTCAAT 
AAAGAGGAGG CCCCACCGAT GAACCCCCTC AACCCCCTCA ACCCGCTGAG CCCCCTCAAC 
CCGCTCAACG CCCACCCCAG CCTCTACCGG AGACCTAGGC CTATTGAGGG CCTGGGCCTG 
GGCCTGGAGA AGTGTGAGGA GGAGCGCATC ATCAGCCGCG GGGGGTCCCT CAAGGGGGAC 
GGAGGGGACC TGTCGAGTGC CCTGGACAAC CAGAGGAGCC CTTTGTCCCT GGGCAAACGG 
GAGCCACCAT GGCTGGCCAG GCCCTGTCAT GGGAACTGTG ATCCGACCCA GCAGGAGGCT 
GGGGGAGGGG AGACCGTGGT GACCTTCGAG GACCGGGCCA GGCACAGGCA GAGCCAGCGG 
CGCAGCCGGC ACCGTCGTGT CAGGACGGAA GGCAAGGAGC TCTCCTCCAC CTCCCGGAGC 
AGGTCTGTCA GCCAGGAGCG GAGTCTGGAT GAGGGGGTGC TCACCGACGG GGAGAAGGAG 
CGAGAGCCCA GGGGCGGCCA TGCAGGCAAA GAACCCACCA TCCAAGAAGA AGACAGAGGC 
CAAGACTTAA GGAGGACGAA CAGTCTGATG GTGCCCAGAG GCTCTGGGCT GGCAGGAGCC 
CTTGATGAGG CCAACACACC CCTAGTCCTG CCCCACCACG ATCTGGAAGT GGGGAAGGAC 
ACGGTGCTGA CGGAGCAGGA GCCGGAGGGC AGCAGCGAGC AGGCCCTGCT GGGGGATGTG 
CAGTTGGACA TTGGCAGGGT CATCAGCCAG AGTGAGCCTG ACCTCTCCTG CGTCACAGCC 
AACACAGACA AGGCCACCAC CGACAGCACC AGCGTCACCG TCGCCATCCC TGATGTGGGC 
CCCTTGATGG ACTCAACTGT GATTCACATT AGCAACAAAA CTGATGGAGA AGCCAGTCCC 
TTGAAGGAGG CTGAGATCAA AGAGGAGGAG GAGGAAATGG AGAAGAAGCA GCAGAAGAAG 
GAGAAGCGTG AGACGGGCAA AGCCATGGTG CCCCACAGCT CTATGTTCAT CTTCAGCACC 
ACCAACCCGA TTCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT TGAGATGTGC 
ATTCTCCTGG TGATCGCCGC CAGCAGCATT GCCCTGGCAG CAGAGGACCC CGTCCTGACC 
AACTCGGAGC GCAACAAAGT CCTGAGGTAT TTTGACTATG TTTTCACTGG CGTGTTCACC 
TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATCC TACAGGATGG GTCCTACTTC 
CGAGACCTGT GGAACATCCT GGACTTTGTG GTGGTGGTTG GTGCATTGGT GGCCTTTGCT 
TTGGCGAACG CTTTGGGAAC AAACAAGGGG CGAGACATCA AGACCATCAA ATCCCTGAGG 
GTGCTCCGAG TTCTGAGGCC ACTGAAGACC ATCAAGCGCT TGCCCAAACT CAAGGCTGTC 
TTTGACTGTG TGGTGACCTC CTTGAAGAAT GTCTTCAACA TACTCATCGT CTATAAGCTC 
TTCATGTTCA TCTTTGCTGT CATTGCGGTT CAGCTCTTCA AGGGAAAATT CTTTTATTGC 
ACAGACAGTT CCAAGGACAC AGAGAAGGAG TGCATAGGCA ACTACGTAGA TCATGAAAAA 
AACAAGATGG AGGTGAAAGG CCGGGAATGG AAGCGCCATG AATTCCACTA CGACAACATT 
ATCTGGGCCC TGCTGACCCT CTTCACCGTC TCCACGGGGG AAGGATGGCC TCAAGTCTTA 
CAGCATTCTG TCGATGTGAC AGAGGAAGAC CGAGGACCAA GCCGCAGCAA CCGCATGGAG 
ATGTCCATCT TCTATGTGGT CTACTTTGTG GTCTTCCCTT TCTTCTTTGT CAATATCTTT 
GTGGCTCTGA TCATCATCAC CTTCCAGGAA CAAGGGGACA AGATGATGGA GGAATGCAGC 
CTGGAGAAGA ACGAGAGGGC GTGCATTGAC TTCGCCATCA GTGCCAAACC TCTCACCCGC 
TACATGCCAC AGAACAGGCA CACCTTCCAG TACCGCGTCT GGCACTTCGT GGTGTCTCCG 
TCCTTTGAGT ACACCATCAT GGCCATGATC GCCCTGAACA CCGTCGTGCT GATGATGAAG 
TACTACTCTG CTCCCTGTAC CTATGAACTG GCCCTGAAGT ACCTGAATAT TGCCTTCACC 
ATGGTGTTTT CCCTGGAATG TGTCCTAAAG ATCATCGCTT TTGGCTTCTT GAACTACTTT 
CGAGACACCT GGAACATCTT TGACTTCATC ACTGTGATTG GCAGTATCAC AGAAATCATA 
CTGACAGACA GCAAGCTGGT AAACACCAGT GGTTTCAACA TGAGCTTTCT GAAGCTCTTC 
CGTGCCGCCC GCCTCATCAA GCTTCTGCGG CAGGGCTACA CCATCCGTAT TTTACTCTGG 
ACCTTCGTGC AGTCCTTTAA GGCCCTCCCC TACGTCTGCC TTTTGATTGC CATGCTTTTC 
TTCATCTATG CCATCATTGG GATGCAGGTA TTTGGAAACA TAAAATTAGA TGAGGAGAGT 
CACATCAACC GGCACAACAA CTTCCGGAGT TTCTTTGGGT CCCTCATGTT ACTCTTCAGG 
AGTGCCACGG GAGAGGCCTG GCAGGAGATA ATGCTGTCGT GCCTCGGGGA GAAAGGCTGC 
GAGCCCGACA CCACTGCGCC ATCGGGGCAG AACGAGAATG AGCGCTGTGG CACCGATCTG 
GCCTATGTTT ACTTTGTCTC CTTCATCTTC TTCTGCTCTT TCTTGATGCT CAACTTGTTT 
GTGGCCGTCA TCATGGACAA CTTCGAGTAC CTGACTCGGG ACTCCTCCAT CTTAGGGCCC 
CACCACTTGG ATGAGTTTGT CCGCGTCTGG GCAGAGTACG ACCGAGCTGC ATGTGGCCGC 
ATCCATTATA CTGAGATGTA TGAAATGCTG ACTCTCATGT CACCACCGCT AGGCCTCGGC 
AAGAGATGTC CCTCCAAAGT GGCATATAAG AGGTTGGTCC TAATGAATAT GCCAGTAGCT 
GAGGACATGA CAGTCCACTT CACCTCCACA CTGATGGCTC TGATCCGGAC AGCCCTGGAC 
ATCAAAATCG CCAAAGGTGG TGCAGACAGG CAGCAGCTAG ACTCGGAGCT GCAAAAGGAG 
ACCCTGGCCA TCTGGCCTCA CCTGTCCCAG AAGATGCTAG ACCTGCTTGT GCCCATGCCC 
AAAGCCTCTG ACCTGACTGT GGGCAAAATC TATGCAGCAA TGATGATCAT GGACTACTAT 
AAACAGAGTA AGGTGAAGAA ACAGAGGCAG CAGCTGGAGG AACAGAAAAA TGCACCCATG 
TTCCAGCGAA TGGAGCCCTC ATCTCTGCCT CAGGAGATCA TTGCTAATGC CAAAGCCCTG 
CCCTATCTCC AGCAGGACCC CGTTTCAGGC CTGAGTGGCC GTAGTGGATA CCCTTCGATG 
AGTCCACTCT CCCCCCAGGA AATATTCCAA TTGGCTTGTA TGGATCCAGC CGATGATGGA 
CAGTTCCAGG AACATCAGTC TCTGGTGGTG ACAGATACTA GCTCCATGAG ACGTTCATTT 
TCCACTATTC GGGATAAGCG TTCAAATTCC TCCTGGTTGG AGGAATTCTC CATGGAGAGA 
AGCAGTGAAA ACACCTACAA GTCACGTCGT CGGAGTTACC ACTCCTCCCT GAGACTGTCA 
GCTCATCGCC TGAATTCTGA CTCAGGCCAC AAGTCTGACA CCCACCGCTC AGGGGGTAGA 
GAGCGGGGAC GATCGAAAGA GCGAAAGCAT CTTCTCTCTC CTGATGTCTC CCGCTGCAAC 
TCAGAGGAGC GAGGGACCCA GGCCGACTGG GAGTCCCCAG AGCGCCAACA GTCCAGGTCT 
CCCAGTGAGG CCAGGTCACA GACCCCCAAC AGACAGGGCA CGGGCTCCCT GAGCGAGAGC 
TCCATCCCCT CCATCTCTGA CACCAGCACC CCAAGACGAA GTCGCCGGCA GCTCCCACCC 
GTCCCACCAA AGCCTCGGCC CCTCCTCTCC TACAGCTCCC TGATTCGACA CTCCGGGAGC 
ATCTCCCCGC CGGCCGACGG AAGCGAGAGG GGCTCCCCGC TGACCCCCCA GGCGCTGGAG 
AGCACGGAGG CCGGCCTGGC GGAGCCCTCG GGCCCCCGGC ACGGGCACGG GCAGGGGCAC 
GGGCAGGGGC ACGGGCAGCA GAGCCAGCAC TGCTCCCCGC AGCGCTACAT CTCCGAGCCC 
TACCTGGCCC TGCACGAAGA CTCCCACGCG TCGGACTGTG GCGAGGAGGA GACGCTCACC 
TTCGAGGCCG CCGTGGCCAC CAGCCTGGGC CGCTCCAACA CCATCGGCTC GGCCCCGCCG 
CTGCGGCACA GCTGGCAGAT GCCCAACGGG CACTATCGGC GGCGGCGGCG CGGGGGGCCG 
GGGCCGGGCG TGATGTGCGG GGCGGTCAGC GACCTCCTGA GTGACACGGA AGAAGACGAC 
AAGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_022420933.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022276641.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe289499
Clone ID Related Accession (Same CDS sequence)	XM_022420933.1
Accession Version	XM_022420933.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGGAC CGAGCCCAGG CCGCAGGCTG CCCGAGCCCG CCCCCTGCCC CGCCGCGGCC 
GGGAGCCCTG AGCCCTGGGC CGCGGGCCGC CGCCGCCACG TCCTGGCCCT GCCTGAGGAG 
GAGGCATGGA GGCCCCAGCA GTGCTCCCTG CTCGAGGCCG ACGCCTCGGA CGAGGTGGGC 
ATGCTGCCGG CCTCCCCGCG CGCCGCCGCC TCCGGCGTCG ACAACTTCTT CCCGGTGCAG 
GAGGGCGAGG GCGCGGGCTG GGAGGGCGGT GCGGCCCTGG ACGCCGGCGC CGGCCCCTTC 
CTCCCGGTGA GCCCCGAGGT GATGAAGCGG CGGCGCGGGG GCCTCATCGA GCAGCGCGAC 
ATCATCAAGG CCCACGAGGC GCACAAGATG CAGAGCACCC CGCAGGCGCG GCGCAAGGAG 
TGGGAGATGG CTCGCTTCGG GGAGGCGGTG GTCGGCAGGC CGGGGTCGGG CGATGGAGAC 
TCGGACCAGA GCAGGAACCG GCAAGGAACC CCGGTGCCGG CCTCGGGGCC GGCGGCCGCC 
TACAAGCAGT CGAAAGCGCA GAGGGCGCGG ACTATGGCTT TGTACAACCC CATTCCCGTC 
CGGCAGAACT GTTTCACCGT CAACAGATCC CTGTTCATCT TCGGAGAAGA TAACATTGTC 
AGGAAATACG CCAAGAAGCT CATCGACTGG CCGCCGTTTG AGTACATGAT TTTGGCAACC 
ATCATTGCCA ACTGCATTGT CTTGGCCCTG GAACAGCATC TTCCTGAGGA TGACAAGACC 
CCGATGTCCC GCAGACTGGA GAAGACAGAG CCGTATTTCA TTGGGATCTT TTGCTTTGAA 
GCCGGTATCA AAATTGTGGC CCTGGGGTTC ATCTTTCATA AGGGCTCGTA CCTCCGCAAT 
GGCTGGAATG TCATGGACTT CATCGTGGTC CTCAGTGGCA TCCTGGCCAC TGCGGGGACT 
CACTTCAACA CACACGTGGA CCTGAGGACC CTCCGGGCTG TGCGTGTCCT GAGACCCCTG 
AAGCTCGTGT CAGGGATTCC TAGCCTGCAG ATCGTGTTGA AGTCTATCAT GAAGGCCATG 
GTGCCTCTTC TGCAAATTGG TCTCCTGCTC TTCTTTGCCA TCTTGATGTT TGCTATCATT 
GGCTTAGAGT TCTACAGTGG GAAGTTGCAC CGAGCATGTT TCACGAACAA TTCAGGTATT 
CTGGAAGGAT TTGACCCCCC TCACCCATGT GGTGTGCAGG GCTGCCCAGC TGGCTACGAA 
TGCCGGGACT GGATTGGCCC CAATGATGGG ATCACCCAGT TTGATAACAT CCTTTTTGCT 
GTGCTGACTG TCTTCCAATG CATCACCATG GAAGGATGGA CCACCGTGCT GTACAATACC 
AATGATGCCT TAGGAGCCAC CTGGAACTGG CTGTACTTCA TCCCCCTCAT CATCATTGGA 
TCCTTCTTTG TTCTCAACCT AGTTCTGGGA GTGCTTTCCG GGGAGTTTGC CAAAGAGAGA 
GAGAGAGTGG AGAACCGGAG AGCTTTCATG AAACTGCGGC GGCAGCAGCA GATTGAGCGG 
GAGCTGAACG GGTACCGCGC CTGGATAGAC AAAGCAGAGG AAGTCATGCT TGCTGAAGAA 
AATAAAAATG CTGGGACGTC AGCCTTAGAA GTGCTTCGAA GGGCAACCAT CAAAAGGAGC 
CGGACAGAGG CCATGACTCG AGACTCCAGT GATGAACACT GTGTCGATAT CTCCTCTGTG 
GGCACACCTC TCGCCCGAGC CAGCATCAAA AGTGCAAAGG TGGATGGGGC CTCCTATTTC 
CGGCACAAAG AAAGGCTTCT GCGCATCTCT ATCCGCCACA TGGTGAAATC CCAGGTGTTT 
TACTGGATTG TGTTGAGCCT TGTGGCTCTC AACACTGCCT GTGTGGCCAT CGTCCATCAT 
AACCAGCCCC AGTGGCTCAC CCATCTCCTC TACTATGCAG AATTTTTGTT TCTGGGACTG 
TTCCTCTTGG AGATGTCCCT GAAGATGTAT GGCATGGGAC CTCGACTTTA TTTTCACTCT 
TCATTCAACT GTTTTGATTT TGGGGTCACA GTGGGCAGCA TCTTTGAAGT GGTCTGGGCA 
ATCTTCAGAC CTGGCACATC TTTTGGAATC AGTGTCTTAC GGGCTCTCCG GCTTCTAAGA 
ATATTTAAAA TAACCAAGTA TTGGGCATCT CTGCGAAATT TGGTGGTCTC CTTGATGAGC 
TCTATGAAGT CTATCATCAG TTTGCTCTTC CTCCTCTTCC TCTTCATCGT TGTCTTTGCT 
CTCCTAGGAA TGCAGCTGTT TGGAGGCAGG TTTAACTTTA ATGATGGGAC TCCTTCAGCA 
AACTTTGATA CCTTCCCTGC AGCCATCATG ACTGTATTCC AGATCCTAAC AGGTGAGGAC 
TGGAATGAGG TGATGTACAA TGGGATCCGT TCCCAGGGTG GAGTTAGCTC AGGCATGTGG 
TCAGCTATCT ATTTCATTGT GCTCACCTTG TTTGGAAACT ACACACTGCT GAATGTGTTC 
TTGGCCATTG CTGTGGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA TGAACAAGAA 
GAAGAGGAGG CTTTTAACCA GAAACATGCA TTGCAGAAGG CCAAGGAGGT CAGCCCGATG 
TCTGCACCCA ACATGCCTTC TATCGAGAGG GAGCGGAGGC GCCGGCACCA CATGTCGGTG 
TGGGAGCAGC GCACCAGCCA GCTGAGAAGG CACATGCAGA TGTCCAGCCA GGAGGCCCTC 
AATAAAGAGG AGGCCCCACC GATGAACCCC CTCAACCCCC TCAACCCGCT GAGCCCCCTC 
AACCCGCTCA ACGCCCACCC CAGCCTCTAC CGGAGACCTA GGCCTATTGA GGGCCTGGGC 
CTGGGCCTGG AGAAGTGTGA GGAGGAGCGC ATCATCAGCC GCGGGGGGTC CCTCAAGGGG 
GACGGAGGGG ACCTGTCGAG TGCCCTGGAC AACCAGAGGA GCCCTTTGTC CCTGGGCAAA 
CGGGAGCCAC CATGGCTGGC CAGGCCCTGT CATGGGAACT GTGATCCGAC CCAGCAGGAG 
GCTGGGGGAG GGGAGACCGT GGTGACCTTC GAGGACCGGG CCAGGCACAG GCAGAGCCAG 
CGGCGCAGCC GGCACCGTCG TGTCAGGACG GAAGGCAAGG AGCTCTCCTC CACCTCCCGG 
AGCAGGTCTG TCAGCCAGGA GCGGAGTCTG GATGAGGGGG TGCTCACCGA CGGGGAGAAG 
GAGCGAGAGC CCAGGGGCGG CCATGCAGGC AAAGAACCCA CCATCCAAGA AGAAGACAGA 
GGCCAAGACT TAAGGAGGAC GAACAGTCTG ATGGTGCCCA GAGGCTCTGG GCTGGCAGGA 
GCCCTTGATG AGGCCAACAC ACCCCTAGTC CTGCCCCACC ACGATCTGGA AGTGGGGAAG 
GACACGGTGC TGACGGAGCA GGAGCCGGAG GGCAGCAGCG AGCAGGCCCT GCTGGGGGAT 
GTGCAGTTGG ACATTGGCAG GGTCATCAGC CAGAGTGAGC CTGACCTCTC CTGCGTCACA 
GCCAACACAG ACAAGGCCAC CACCGACAGC ACCAGCGTCA CCGTCGCCAT CCCTGATGTG 
GGCCCCTTGA TGGACTCAAC TGTGATTCAC ATTAGCAACA AAACTGATGG AGAAGCCAGT 
CCCTTGAAGG AGGCTGAGAT CAAAGAGGAG GAGGAGGAAA TGGAGAAGAA GCAGCAGAAG 
AAGGAGAAGC GTGAGACGGG CAAAGCCATG GTGCCCCACA GCTCTATGTT CATCTTCAGC 
ACCACCAACC CGATTCGCAG GGCCTGCCAC TACATCGTGA ACCTGCGCTA CTTTGAGATG 
TGCATTCTCC TGGTGATCGC CGCCAGCAGC ATTGCCCTGG CAGCAGAGGA CCCCGTCCTG 
ACCAACTCGG AGCGCAACAA AGTCCTGAGG TATTTTGACT ATGTTTTCAC TGGCGTGTTC 
ACCTTTGAGA TGGTTATAAA GATGATAGAC CAGGGCTTGA TCCTACAGGA TGGGTCCTAC 
TTCCGAGACC TGTGGAACAT CCTGGACTTT GTGGTGGTGG TTGGTGCATT GGTGGCCTTT 
GCTTTGGCGA ACGCTTTGGG AACAAACAAG GGGCGAGACA TCAAGACCAT CAAATCCCTG 
AGGGTGCTCC GAGTTCTGAG GCCACTGAAG ACCATCAAGC GCTTGCCCAA ACTCAAGGCT 
GTCTTTGACT GTGTGGTGAC CTCCTTGAAG AATGTCTTCA ACATACTCAT CGTCTATAAG 
CTCTTCATGT TCATCTTTGC TGTCATTGCG GTTCAGCTCT TCAAGGGAAA ATTCTTTTAT 
TGCACAGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTACGT AGATCATGAA 
AAAAACAAGA TGGAGGTGAA AGGCCGGGAA TGGAAGCGCC ATGAATTCCA CTACGACAAC 
ATTATCTGGG CCCTGCTGAC CCTCTTCACC GTCTCCACGG GGGAAGGATG GCCTCAAGTC 
TTACAGCATT CTGTCGATGT GACAGAGGAA GACCGAGGAC CAAGCCGCAG CAACCGCATG 
GAGATGTCCA TCTTCTATGT GGTCTACTTT GTGGTCTTCC CTTTCTTCTT TGTCAATATC 
TTTGTGGCTC TGATCATCAT CACCTTCCAG GAACAAGGGG ACAAGATGAT GGAGGAATGC 
AGCCTGGAGA AGAACGAGAG GGCGTGCATT GACTTCGCCA TCAGTGCCAA ACCTCTCACC 
CGCTACATGC CACAGAACAG GCACACCTTC CAGTACCGCG TCTGGCACTT CGTGGTGTCT 
CCGTCCTTTG AGTACACCAT CATGGCCATG ATCGCCCTGA ACACCGTCGT GCTGATGATG 
AAGTACTACT CTGCTCCCTG TACCTATGAA CTGGCCCTGA AGTACCTGAA TATTGCCTTC 
ACCATGGTGT TTTCCCTGGA ATGTGTCCTA AAGATCATCG CTTTTGGCTT CTTGAACTAC 
TTTCGAGACA CCTGGAACAT CTTTGACTTC ATCACTGTGA TTGGCAGTAT CACAGAAATC 
ATACTGACAG ACAGCAAGCT GGTAAACACC AGTGGTTTCA ACATGAGCTT TCTGAAGCTC 
TTCCGTGCCG CCCGCCTCAT CAAGCTTCTG CGGCAGGGCT ACACCATCCG TATTTTACTC 
TGGACCTTCG TGCAGTCCTT TAAGGCCCTC CCCTACGTCT GCCTTTTGAT TGCCATGCTT 
TTCTTCATCT ATGCCATCAT TGGGATGCAG GTATTTGGAA ACATAAAATT AGATGAGGAG 
AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GGTCCCTCAT GTTACTCTTC 
AGGAGTGCCA CGGGAGAGGC CTGGCAGGAG ATAATGCTGT CGTGCCTCGG GGAGAAAGGC 
TGCGAGCCCG ACACCACTGC GCCATCGGGG CAGAACGAGA ATGAGCGCTG TGGCACCGAT 
CTGGCCTATG TTTACTTTGT CTCCTTCATC TTCTTCTGCT CTTTCTTGAT GCTCAACTTG 
TTTGTGGCCG TCATCATGGA CAACTTCGAG TACCTGACTC GGGACTCCTC CATCTTAGGG 
CCCCACCACT TGGATGAGTT TGTCCGCGTC TGGGCAGAGT ACGACCGAGC TGCATGTGGC 
CGCATCCATT ATACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCACC GCTAGGCCTC 
GGCAAGAGAT GTCCCTCCAA AGTGGCATAT AAGAGGTTGG TCCTAATGAA TATGCCAGTA 
GCTGAGGACA TGACAGTCCA CTTCACCTCC ACACTGATGG CTCTGATCCG GACAGCCCTG 
GACATCAAAA TCGCCAAAGG TGGTGCAGAC AGGCAGCAGC TAGACTCGGA GCTGCAAAAG 
GAGACCCTGG CCATCTGGCC TCACCTGTCC CAGAAGATGC TAGACCTGCT TGTGCCCATG 
CCCAAAGCCT CTGACCTGAC TGTGGGCAAA ATCTATGCAG CAATGATGAT CATGGACTAC 
TATAAACAGA GTAAGGTGAA GAAACAGAGG CAGCAGCTGG AGGAACAGAA AAATGCACCC 
ATGTTCCAGC GAATGGAGCC CTCATCTCTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC 
CTGCCCTATC TCCAGCAGGA CCCCGTTTCA GGCCTGAGTG GCCGTAGTGG ATACCCTTCG 
ATGAGTCCAC TCTCCCCCCA GGAAATATTC CAATTGGCTT GTATGGATCC AGCCGATGAT 
GGACAGTTCC AGGAACATCA GTCTCTGGTG GTGACAGATA CTAGCTCCAT GAGACGTTCA 
TTTTCCACTA TTCGGGATAA GCGTTCAAAT TCCTCCTGGT TGGAGGAATT CTCCATGGAG 
AGAAGCAGTG AAAACACCTA CAAGTCACGT CGTCGGAGTT ACCACTCCTC CCTGAGACTG 
TCAGCTCATC GCCTGAATTC TGACTCAGGC CACAAGTCTG ACACCCACCG CTCAGGGGGT 
AGAGAGCGGG GACGATCGAA AGAGCGAAAG CATCTTCTCT CTCCTGATGT CTCCCGCTGC 
AACTCAGAGG AGCGAGGGAC CCAGGCCGAC TGGGAGTCCC CAGAGCGCCA ACAGTCCAGG 
TCTCCCAGTG AGGCCAGGTC ACAGACCCCC AACAGACAGG GCACGGGCTC CCTGAGCGAG 
AGCTCCATCC CCTCCATCTC TGACACCAGC ACCCCAAGAC GAAGTCGCCG GCAGCTCCCA 
CCCGTCCCAC CAAAGCCTCG GCCCCTCCTC TCCTACAGCT CCCTGATTCG ACACTCCGGG 
AGCATCTCCC CGCCGGCCGA CGGAAGCGAG AGGGGCTCCC CGCTGACCCC CCAGGCGCTG 
GAGAGCACGG AGGCCGGCCT GGCGGAGCCC TCGGGCCCCC GGCACGGGCA CGGGCAGGGG 
CACGGGCAGG GGCACGGGCA GCAGAGCCAG CACTGCTCCC CGCAGCGCTA CATCTCCGAG 
CCCTACCTGG CCCTGCACGA AGACTCCCAC GCGTCGGACT GTGGCGAGGA GGAGACGCTC 
ACCTTCGAGG CCGCCGTGGC CACCAGCCTG GGCCGCTCCA ACACCATCGG CTCGGCCCCG 
CCGCTGCGGC ACAGCTGGCA GATGCCCAAC GGGCACTATC GGCGGCGGCG GCGCGGGGGG 
CCGGGGCCGG GCGTGATGTG CGGGGCGGTC AGCGACCTCC TGAGTGACAC GGAAGAAGAC 
GACAAGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276641.1
CDS	4..7215
Translation	MRGPSPGRRLPEPAPCPAAAGSPEPWAAGRRRHVLALPEEEAWRPQQCSLLEADASDEVGMLPASPRAAASGVDNFFPVQEGEGAGWEGGAALDAGAGPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFTNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLGLGLEKCEEERIISRGGSLKGDGGDLSSALDNQRSPLSLGKREPPWLARPCHGNCDPTQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKELSSTSRSRSVSQERSLDEGVLTDGEKEREPRGGHAGKEPTIQEEDRGQDLRRTNSLMVPRGSGLAGALDEANTPLVLPHHDLEVGKDTVLTEQEPEGSSEQALLGDVQLDIGRVISQSEPDLSCVTANTDKATTDSTSVTVAIPDVGPLMDSTVIHISNKTDGEASPLKEAEIKEEEEEMEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEHQSLVVTDTSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERQQSRSPSEARSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHSGSISPPADGSERGSPLTPQALESTEAGLAEPSGPRHGHGQGHGQGHGQQSQHCSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGVMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_022420933.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022420933.1

ORF Insert Sequence:

ATGCGTGGAC CGAGCCCAGG CCGCAGGCTG CCCGAGCCCG CCCCCTGCCC CGCCGCGGCC 
GGGAGCCCTG AGCCCTGGGC CGCGGGCCGC CGCCGCCACG TCCTGGCCCT GCCTGAGGAG 
GAGGCATGGA GGCCCCAGCA GTGCTCCCTG CTCGAGGCCG ACGCCTCGGA CGAGGTGGGC 
ATGCTGCCGG CCTCCCCGCG CGCCGCCGCC TCCGGCGTCG ACAACTTCTT CCCGGTGCAG 
GAGGGCGAGG GCGCGGGCTG GGAGGGCGGT GCGGCCCTGG ACGCCGGCGC CGGCCCCTTC 
CTCCCGGTGA GCCCCGAGGT GATGAAGCGG CGGCGCGGGG GCCTCATCGA GCAGCGCGAC 
ATCATCAAGG CCCACGAGGC GCACAAGATG CAGAGCACCC CGCAGGCGCG GCGCAAGGAG 
TGGGAGATGG CTCGCTTCGG GGAGGCGGTG GTCGGCAGGC CGGGGTCGGG CGATGGAGAC 
TCGGACCAGA GCAGGAACCG GCAAGGAACC CCGGTGCCGG CCTCGGGGCC GGCGGCCGCC 
TACAAGCAGT CGAAAGCGCA GAGGGCGCGG ACTATGGCTT TGTACAACCC CATTCCCGTC 
CGGCAGAACT GTTTCACCGT CAACAGATCC CTGTTCATCT TCGGAGAAGA TAACATTGTC 
AGGAAATACG CCAAGAAGCT CATCGACTGG CCGCCGTTTG AGTACATGAT TTTGGCAACC 
ATCATTGCCA ACTGCATTGT CTTGGCCCTG GAACAGCATC TTCCTGAGGA TGACAAGACC 
CCGATGTCCC GCAGACTGGA GAAGACAGAG CCGTATTTCA TTGGGATCTT TTGCTTTGAA 
GCCGGTATCA AAATTGTGGC CCTGGGGTTC ATCTTTCATA AGGGCTCGTA CCTCCGCAAT 
GGCTGGAATG TCATGGACTT CATCGTGGTC CTCAGTGGCA TCCTGGCCAC TGCGGGGACT 
CACTTCAACA CACACGTGGA CCTGAGGACC CTCCGGGCTG TGCGTGTCCT GAGACCCCTG 
AAGCTCGTGT CAGGGATTCC TAGCCTGCAG ATCGTGTTGA AGTCTATCAT GAAGGCCATG 
GTGCCTCTTC TGCAAATTGG TCTCCTGCTC TTCTTTGCCA TCTTGATGTT TGCTATCATT 
GGCTTAGAGT TCTACAGTGG GAAGTTGCAC CGAGCATGTT TCACGAACAA TTCAGGTATT 
CTGGAAGGAT TTGACCCCCC TCACCCATGT GGTGTGCAGG GCTGCCCAGC TGGCTACGAA 
TGCCGGGACT GGATTGGCCC CAATGATGGG ATCACCCAGT TTGATAACAT CCTTTTTGCT 
GTGCTGACTG TCTTCCAATG CATCACCATG GAAGGATGGA CCACCGTGCT GTACAATACC 
AATGATGCCT TAGGAGCCAC CTGGAACTGG CTGTACTTCA TCCCCCTCAT CATCATTGGA 
TCCTTCTTTG TTCTCAACCT AGTTCTGGGA GTGCTTTCCG GGGAGTTTGC CAAAGAGAGA 
GAGAGAGTGG AGAACCGGAG AGCTTTCATG AAACTGCGGC GGCAGCAGCA GATTGAGCGG 
GAGCTGAACG GGTACCGCGC CTGGATAGAC AAAGCAGAGG AAGTCATGCT TGCTGAAGAA 
AATAAAAATG CTGGGACGTC AGCCTTAGAA GTGCTTCGAA GGGCAACCAT CAAAAGGAGC 
CGGACAGAGG CCATGACTCG AGACTCCAGT GATGAACACT GTGTCGATAT CTCCTCTGTG 
GGCACACCTC TCGCCCGAGC CAGCATCAAA AGTGCAAAGG TGGATGGGGC CTCCTATTTC 
CGGCACAAAG AAAGGCTTCT GCGCATCTCT ATCCGCCACA TGGTGAAATC CCAGGTGTTT 
TACTGGATTG TGTTGAGCCT TGTGGCTCTC AACACTGCCT GTGTGGCCAT CGTCCATCAT 
AACCAGCCCC AGTGGCTCAC CCATCTCCTC TACTATGCAG AATTTTTGTT TCTGGGACTG 
TTCCTCTTGG AGATGTCCCT GAAGATGTAT GGCATGGGAC CTCGACTTTA TTTTCACTCT 
TCATTCAACT GTTTTGATTT TGGGGTCACA GTGGGCAGCA TCTTTGAAGT GGTCTGGGCA 
ATCTTCAGAC CTGGCACATC TTTTGGAATC AGTGTCTTAC GGGCTCTCCG GCTTCTAAGA 
ATATTTAAAA TAACCAAGTA TTGGGCATCT CTGCGAAATT TGGTGGTCTC CTTGATGAGC 
TCTATGAAGT CTATCATCAG TTTGCTCTTC CTCCTCTTCC TCTTCATCGT TGTCTTTGCT 
CTCCTAGGAA TGCAGCTGTT TGGAGGCAGG TTTAACTTTA ATGATGGGAC TCCTTCAGCA 
AACTTTGATA CCTTCCCTGC AGCCATCATG ACTGTATTCC AGATCCTAAC AGGTGAGGAC 
TGGAATGAGG TGATGTACAA TGGGATCCGT TCCCAGGGTG GAGTTAGCTC AGGCATGTGG 
TCAGCTATCT ATTTCATTGT GCTCACCTTG TTTGGAAACT ACACACTGCT GAATGTGTTC 
TTGGCCATTG CTGTGGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA TGAACAAGAA 
GAAGAGGAGG CTTTTAACCA GAAACATGCA TTGCAGAAGG CCAAGGAGGT CAGCCCGATG 
TCTGCACCCA ACATGCCTTC TATCGAGAGG GAGCGGAGGC GCCGGCACCA CATGTCGGTG 
TGGGAGCAGC GCACCAGCCA GCTGAGAAGG CACATGCAGA TGTCCAGCCA GGAGGCCCTC 
AATAAAGAGG AGGCCCCACC GATGAACCCC CTCAACCCCC TCAACCCGCT GAGCCCCCTC 
AACCCGCTCA ACGCCCACCC CAGCCTCTAC CGGAGACCTA GGCCTATTGA GGGCCTGGGC 
CTGGGCCTGG AGAAGTGTGA GGAGGAGCGC ATCATCAGCC GCGGGGGGTC CCTCAAGGGG 
GACGGAGGGG ACCTGTCGAG TGCCCTGGAC AACCAGAGGA GCCCTTTGTC CCTGGGCAAA 
CGGGAGCCAC CATGGCTGGC CAGGCCCTGT CATGGGAACT GTGATCCGAC CCAGCAGGAG 
GCTGGGGGAG GGGAGACCGT GGTGACCTTC GAGGACCGGG CCAGGCACAG GCAGAGCCAG 
CGGCGCAGCC GGCACCGTCG TGTCAGGACG GAAGGCAAGG AGCTCTCCTC CACCTCCCGG 
AGCAGGTCTG TCAGCCAGGA GCGGAGTCTG GATGAGGGGG TGCTCACCGA CGGGGAGAAG 
GAGCGAGAGC CCAGGGGCGG CCATGCAGGC AAAGAACCCA CCATCCAAGA AGAAGACAGA 
GGCCAAGACT TAAGGAGGAC GAACAGTCTG ATGGTGCCCA GAGGCTCTGG GCTGGCAGGA 
GCCCTTGATG AGGCCAACAC ACCCCTAGTC CTGCCCCACC ACGATCTGGA AGTGGGGAAG 
GACACGGTGC TGACGGAGCA GGAGCCGGAG GGCAGCAGCG AGCAGGCCCT GCTGGGGGAT 
GTGCAGTTGG ACATTGGCAG GGTCATCAGC CAGAGTGAGC CTGACCTCTC CTGCGTCACA 
GCCAACACAG ACAAGGCCAC CACCGACAGC ACCAGCGTCA CCGTCGCCAT CCCTGATGTG 
GGCCCCTTGA TGGACTCAAC TGTGATTCAC ATTAGCAACA AAACTGATGG AGAAGCCAGT 
CCCTTGAAGG AGGCTGAGAT CAAAGAGGAG GAGGAGGAAA TGGAGAAGAA GCAGCAGAAG 
AAGGAGAAGC GTGAGACGGG CAAAGCCATG GTGCCCCACA GCTCTATGTT CATCTTCAGC 
ACCACCAACC CGATTCGCAG GGCCTGCCAC TACATCGTGA ACCTGCGCTA CTTTGAGATG 
TGCATTCTCC TGGTGATCGC CGCCAGCAGC ATTGCCCTGG CAGCAGAGGA CCCCGTCCTG 
ACCAACTCGG AGCGCAACAA AGTCCTGAGG TATTTTGACT ATGTTTTCAC TGGCGTGTTC 
ACCTTTGAGA TGGTTATAAA GATGATAGAC CAGGGCTTGA TCCTACAGGA TGGGTCCTAC 
TTCCGAGACC TGTGGAACAT CCTGGACTTT GTGGTGGTGG TTGGTGCATT GGTGGCCTTT 
GCTTTGGCGA ACGCTTTGGG AACAAACAAG GGGCGAGACA TCAAGACCAT CAAATCCCTG 
AGGGTGCTCC GAGTTCTGAG GCCACTGAAG ACCATCAAGC GCTTGCCCAA ACTCAAGGCT 
GTCTTTGACT GTGTGGTGAC CTCCTTGAAG AATGTCTTCA ACATACTCAT CGTCTATAAG 
CTCTTCATGT TCATCTTTGC TGTCATTGCG GTTCAGCTCT TCAAGGGAAA ATTCTTTTAT 
TGCACAGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTACGT AGATCATGAA 
AAAAACAAGA TGGAGGTGAA AGGCCGGGAA TGGAAGCGCC ATGAATTCCA CTACGACAAC 
ATTATCTGGG CCCTGCTGAC CCTCTTCACC GTCTCCACGG GGGAAGGATG GCCTCAAGTC 
TTACAGCATT CTGTCGATGT GACAGAGGAA GACCGAGGAC CAAGCCGCAG CAACCGCATG 
GAGATGTCCA TCTTCTATGT GGTCTACTTT GTGGTCTTCC CTTTCTTCTT TGTCAATATC 
TTTGTGGCTC TGATCATCAT CACCTTCCAG GAACAAGGGG ACAAGATGAT GGAGGAATGC 
AGCCTGGAGA AGAACGAGAG GGCGTGCATT GACTTCGCCA TCAGTGCCAA ACCTCTCACC 
CGCTACATGC CACAGAACAG GCACACCTTC CAGTACCGCG TCTGGCACTT CGTGGTGTCT 
CCGTCCTTTG AGTACACCAT CATGGCCATG ATCGCCCTGA ACACCGTCGT GCTGATGATG 
AAGTACTACT CTGCTCCCTG TACCTATGAA CTGGCCCTGA AGTACCTGAA TATTGCCTTC 
ACCATGGTGT TTTCCCTGGA ATGTGTCCTA AAGATCATCG CTTTTGGCTT CTTGAACTAC 
TTTCGAGACA CCTGGAACAT CTTTGACTTC ATCACTGTGA TTGGCAGTAT CACAGAAATC 
ATACTGACAG ACAGCAAGCT GGTAAACACC AGTGGTTTCA ACATGAGCTT TCTGAAGCTC 
TTCCGTGCCG CCCGCCTCAT CAAGCTTCTG CGGCAGGGCT ACACCATCCG TATTTTACTC 
TGGACCTTCG TGCAGTCCTT TAAGGCCCTC CCCTACGTCT GCCTTTTGAT TGCCATGCTT 
TTCTTCATCT ATGCCATCAT TGGGATGCAG GTATTTGGAA ACATAAAATT AGATGAGGAG 
AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GGTCCCTCAT GTTACTCTTC 
AGGAGTGCCA CGGGAGAGGC CTGGCAGGAG ATAATGCTGT CGTGCCTCGG GGAGAAAGGC 
TGCGAGCCCG ACACCACTGC GCCATCGGGG CAGAACGAGA ATGAGCGCTG TGGCACCGAT 
CTGGCCTATG TTTACTTTGT CTCCTTCATC TTCTTCTGCT CTTTCTTGAT GCTCAACTTG 
TTTGTGGCCG TCATCATGGA CAACTTCGAG TACCTGACTC GGGACTCCTC CATCTTAGGG 
CCCCACCACT TGGATGAGTT TGTCCGCGTC TGGGCAGAGT ACGACCGAGC TGCATGTGGC 
CGCATCCATT ATACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCACC GCTAGGCCTC 
GGCAAGAGAT GTCCCTCCAA AGTGGCATAT AAGAGGTTGG TCCTAATGAA TATGCCAGTA 
GCTGAGGACA TGACAGTCCA CTTCACCTCC ACACTGATGG CTCTGATCCG GACAGCCCTG 
GACATCAAAA TCGCCAAAGG TGGTGCAGAC AGGCAGCAGC TAGACTCGGA GCTGCAAAAG 
GAGACCCTGG CCATCTGGCC TCACCTGTCC CAGAAGATGC TAGACCTGCT TGTGCCCATG 
CCCAAAGCCT CTGACCTGAC TGTGGGCAAA ATCTATGCAG CAATGATGAT CATGGACTAC 
TATAAACAGA GTAAGGTGAA GAAACAGAGG CAGCAGCTGG AGGAACAGAA AAATGCACCC 
ATGTTCCAGC GAATGGAGCC CTCATCTCTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC 
CTGCCCTATC TCCAGCAGGA CCCCGTTTCA GGCCTGAGTG GCCGTAGTGG ATACCCTTCG 
ATGAGTCCAC TCTCCCCCCA GGAAATATTC CAATTGGCTT GTATGGATCC AGCCGATGAT 
GGACAGTTCC AGGAACATCA GTCTCTGGTG GTGACAGATA CTAGCTCCAT GAGACGTTCA 
TTTTCCACTA TTCGGGATAA GCGTTCAAAT TCCTCCTGGT TGGAGGAATT CTCCATGGAG 
AGAAGCAGTG AAAACACCTA CAAGTCACGT CGTCGGAGTT ACCACTCCTC CCTGAGACTG 
TCAGCTCATC GCCTGAATTC TGACTCAGGC CACAAGTCTG ACACCCACCG CTCAGGGGGT 
AGAGAGCGGG GACGATCGAA AGAGCGAAAG CATCTTCTCT CTCCTGATGT CTCCCGCTGC 
AACTCAGAGG AGCGAGGGAC CCAGGCCGAC TGGGAGTCCC CAGAGCGCCA ACAGTCCAGG 
TCTCCCAGTG AGGCCAGGTC ACAGACCCCC AACAGACAGG GCACGGGCTC CCTGAGCGAG 
AGCTCCATCC CCTCCATCTC TGACACCAGC ACCCCAAGAC GAAGTCGCCG GCAGCTCCCA 
CCCGTCCCAC CAAAGCCTCG GCCCCTCCTC TCCTACAGCT CCCTGATTCG ACACTCCGGG 
AGCATCTCCC CGCCGGCCGA CGGAAGCGAG AGGGGCTCCC CGCTGACCCC CCAGGCGCTG 
GAGAGCACGG AGGCCGGCCT GGCGGAGCCC TCGGGCCCCC GGCACGGGCA CGGGCAGGGG 
CACGGGCAGG GGCACGGGCA GCAGAGCCAG CACTGCTCCC CGCAGCGCTA CATCTCCGAG 
CCCTACCTGG CCCTGCACGA AGACTCCCAC GCGTCGGACT GTGGCGAGGA GGAGACGCTC 
ACCTTCGAGG CCGCCGTGGC CACCAGCCTG GGCCGCTCCA ACACCATCGG CTCGGCCCCG 
CCGCTGCGGC ACAGCTGGCA GATGCCCAAC GGGCACTATC GGCGGCGGCG GCGCGGGGGG 
CCGGGGCCGG GCGTGATGTG CGGGGCGGTC AGCGACCTCC TGAGTGACAC GGAAGAAGAC 
GACAAGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_022420934.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022276642.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCe289500
Clone ID Related Accession (Same CDS sequence)	XM_022420934.1
Accession Version	XM_022420934.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGGAC CGAGCCCAGG CCGCAGGCTG CCCGAGCCCG CCCCCTGCCC CGCCGCGGCC 
GGGAGCCCTG AGCCCTGGGC CGCGGGCCGC CGCCGCCACG TCCTGGCCCT GCCTGAGGAG 
GAGGCATGGA GGCCCCAGCA GTGCTCCCTG CTCGAGGCCG ACGCCTCGGA CGAGGTGGGC 
ATGCTGCCGG CCTCCCCGCG CGCCGCCGCC TCCGGCGTCG ACAACTTCTT CCCGGTGCAG 
GAGGGCGAGG GCGCGGGCTG GGAGGGCGGT GCGGCCCTGG ACGCCGGCGC CGGCCCCTTC 
CTCCCGGTGA GCCCCGAGGT GATGAAGCGG CGGCGCGGGG GCCTCATCGA GCAGCGCGAC 
ATCATCAAGG CCCACGAGGC GCACAAGATG CAGAGCACCC CGCAGGCGCG GCGCAAGGAG 
TGGGAGATGG CTCGCTTCGG GGAGGCGGTG GTCGGCAGGC CGGGGTCGGG CGATGGAGAC 
TCGGACCAGA GCAGGAACCG GCAAGGAACC CCGGTGCCGG CCTCGGGGCC GGCGGCCGCC 
TACAAGCAGT CGAAAGCGCA GAGGGCGCGG ACTATGGCTT TGTACAACCC CATTCCCGTC 
CGGCAGAACT GTTTCACCGT CAACAGATCC CTGTTCATCT TCGGAGAAGA TAACATTGTC 
AGGAAATACG CCAAGAAGCT CATCGACTGG CCGCCGTTTG AGTACATGAT TTTGGCAACC 
ATCATTGCCA ACTGCATTGT CTTGGCCCTG GAACAGCATC TTCCTGAGGA TGACAAGACC 
CCGATGTCCC GCAGACTGGA GAAGACAGAG CCGTATTTCA TTGGGATCTT TTGCTTTGAA 
GCCGGTATCA AAATTGTGGC CCTGGGGTTC ATCTTTCATA AGGGCTCGTA CCTCCGCAAT 
GGCTGGAATG TCATGGACTT CATCGTGGTC CTCAGTGGCA TCCTGGCCAC TGCGGGGACT 
CACTTCAACA CACACGTGGA CCTGAGGACC CTCCGGGCTG TGCGTGTCCT GAGACCCCTG 
AAGCTCGTGT CAGGGATTCC TAGCCTGCAG ATCGTGTTGA AGTCTATCAT GAAGGCCATG 
GTGCCTCTTC TGCAAATTGG TCTCCTGCTC TTCTTTGCCA TCTTGATGTT TGCTATCATT 
GGCTTAGAGT TCTACAGTGG GAAGTTGCAC CGAGCATGTT TCACGAACAA TTCAGGTATT 
CTGGAAGGAT TTGACCCCCC TCACCCATGT GGTGTGCAGG GCTGCCCAGC TGGCTACGAA 
TGCCGGGACT GGATTGGCCC CAATGATGGG ATCACCCAGT TTGATAACAT CCTTTTTGCT 
GTGCTGACTG TCTTCCAATG CATCACCATG GAAGGATGGA CCACCGTGCT GTACAATACC 
AATGATGCCT TAGGAGCCAC CTGGAACTGG CTGTACTTCA TCCCCCTCAT CATCATTGGA 
TCCTTCTTTG TTCTCAACCT AGTTCTGGGA GTGCTTTCCG GGGAGTTTGC CAAAGAGAGA 
GAGAGAGTGG AGAACCGGAG AGCTTTCATG AAACTGCGGC GGCAGCAGCA GATTGAGCGG 
GAGCTGAACG GGTACCGCGC CTGGATAGAC AAAGCAGAGG AAGTCATGCT TGCTGAAGAA 
AATAAAAATG CTGGGACGTC AGCCTTAGAA GTGCTTCGAA GGGCAACCAT CAAAAGGAGC 
CGGACAGAGG CCATGACTCG AGACTCCAGT GATGAACACT GTGTCGATAT CTCCTCTGTG 
GGCACACCTC TCGCCCGAGC CAGCATCAAA AGTGCAAAGG TGGATGGGGC CTCCTATTTC 
CGGCACAAAG AAAGGCTTCT GCGCATCTCT ATCCGCCACA TGGTGAAATC CCAGGTGTTT 
TACTGGATTG TGTTGAGCCT TGTGGCTCTC AACACTGCCT GTGTGGCCAT CGTCCATCAT 
AACCAGCCCC AGTGGCTCAC CCATCTCCTC TACTATGCAG AATTTTTGTT TCTGGGACTG 
TTCCTCTTGG AGATGTCCCT GAAGATGTAT GGCATGGGAC CTCGACTTTA TTTTCACTCT 
TCATTCAACT GTTTTGATTT TGGGGTCACA GTGGGCAGCA TCTTTGAAGT GGTCTGGGCA 
ATCTTCAGAC CTGGCACATC TTTTGGAATC AGTGTCTTAC GGGCTCTCCG GCTTCTAAGA 
ATATTTAAAA TAACCAAGTA TTGGGCATCT CTGCGAAATT TGGTGGTCTC CTTGATGAGC 
TCTATGAAGT CTATCATCAG TTTGCTCTTC CTCCTCTTCC TCTTCATCGT TGTCTTTGCT 
CTCCTAGGAA TGCAGCTGTT TGGAGGCAGG TTTAACTTTA ATGATGGGAC TCCTTCAGCA 
AACTTTGATA CCTTCCCTGC AGCCATCATG ACTGTATTCC AGATCCTAAC AGGTGAGGAC 
TGGAATGAGG TGATGTACAA TGGGATCCGT TCCCAGGGTG GAGTTAGCTC AGGCATGTGG 
TCAGCTATCT ATTTCATTGT GCTCACCTTG TTTGGAAACT ACACACTGCT GAATGTGTTC 
TTGGCCATTG CTGTGGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA TGAACAAGAA 
GAAGAGGAGG CTTTTAACCA GAAACATGCA TTGCAGAAGG CCAAGGAGGT CAGCCCGATG 
TCTGCACCCA ACATGCCTTC TATCGAGAGG GAGCGGAGGC GCCGGCACCA CATGTCGGTG 
TGGGAGCAGC GCACCAGCCA GCTGAGAAGG CACATGCAGA TGTCCAGCCA GGAGGCCCTC 
AATAAAGAGG AGGCCCCACC GATGAACCCC CTCAACCCCC TCAACCCGCT GAGCCCCCTC 
AACCCGCTCA ACGCCCACCC CAGCCTCTAC CGGAGACCTA GGCCTATTGA GGGCCTGGGC 
CTGGGCCTGG AGAAGTGTGA GGAGGAGCGC ATCATCAGCC GCGGGGGGTC CCTCAAGGGG 
GACGGAGGGG ACCTGTCGAG TGCCCTGGAC AACCAGAGGA GCCCTTTGTC CCTGGGCAAA 
CGGGAGCCAC CATGGCTGGC CAGGCCCTGT CATGGGAACT GTGATCCGAC CCAGCAGGAG 
GCTGGGGGAG GGGAGACCGT GGTGACCTTC GAGGACCGGG CCAGGCACAG GCAGAGCCAG 
CGGCGCAGCC GGCACCGTCG TGTCAGGACG GAAGGCAAGG AGCTCTCCTC CACCTCCCGG 
AGCAGGTCTG TCAGCCAGGA GCGGAGTCTG GATGAGGGGG TGCTCACCGA CGGGGAGAAG 
GAGCGAGAGC CCAGGGGCGG CCATGCAGGC AAAGAACCCA CCATCCAAGA AGAAGACAGA 
GGCCAAGACT TAAGGAGGAC GAACAGTCTG ATGGTGCCCA GAGGCTCTGG GCTGGCAGGA 
GCCCTTGATG AGGCCAACAC ACCCCTAGTC CTGCCCCACC ACGATCTGGA AGTGGGGAAG 
GACACGGTGC TGACGGAGCA GGAGCCGGAG GGCAGCAGCG AGCAGGCCCT GCTGGGGGAT 
GTGCAGTTGG ACATTGGCAG GGTCATCAGC CAGAGTGAGC CTGACCTCTC CTGCGTCACA 
GCCAACACAG ACAAGGCCAC CACCGACAGC ACCAGCGTCA CCGTCGCCAT CCCTGATGTG 
GGCCCCTTGA TGGACTCAAC TGTGATTCAC ATTAGCAACA AAACTGATGG AGAAGCCAGT 
CCCTTGAAGG AGGCTGAGAT CAAAGAGGAG GAGGAGGAAA TGGAGAAGAA GCAGCAGAAG 
AAGGAGAAGC GTGAGACGGG CAAAGCCATG GTGCCCCACA GCTCTATGTT CATCTTCAGC 
ACCACCAACC CGATTCGCAG GGCCTGCCAC TACATCGTGA ACCTGCGCTA CTTTGAGATG 
TGCATTCTCC TGGTGATCGC CGCCAGCAGC ATTGCCCTGG CAGCAGAGGA CCCCGTCCTG 
ACCAACTCGG AGCGCAACAA AGTCCTGAGG TATTTTGACT ATGTTTTCAC TGGCGTGTTC 
ACCTTTGAGA TGGTTATAAA GATGATAGAC CAGGGCTTGA TCCTACAGGA TGGGTCCTAC 
TTCCGAGACC TGTGGAACAT CCTGGACTTT GTGGTGGTGG TTGGTGCATT GGTGGCCTTT 
GCTTTGGCGA ACGCTTTGGG AACAAACAAG GGGCGAGACA TCAAGACCAT CAAATCCCTG 
AGGGTGCTCC GAGTTCTGAG GCCACTGAAG ACCATCAAGC GCTTGCCCAA ACTCAAGGCT 
GTCTTTGACT GTGTGGTGAC CTCCTTGAAG AATGTCTTCA ACATACTCAT CGTCTATAAG 
CTCTTCATGT TCATCTTTGC TGTCATTGCG GTTCAGCTCT TCAAGGGAAA ATTCTTTTAT 
TGCACAGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTACGT AGATCATGAA 
AAAAACAAGA TGGAGGTGAA AGGCCGGGAA TGGAAGCGCC ATGAATTCCA CTACGACAAC 
ATTATCTGGG CCCTGCTGAC CCTCTTCACC GTCTCCACGG GGGAAGGATG GCCTCAAGTC 
TTACAGCATT CTGTCGATGT GACAGAGGAA GACCGAGGAC CAAGCCGCAG CAACCGCATG 
GAGATGTCCA TCTTCTATGT GGTCTACTTT GTGGTCTTCC CTTTCTTCTT TGTCAATATC 
TTTGTGGCTC TGATCATCAT CACCTTCCAG GAACAAGGGG ACAAGATGAT GGAGGAATGC 
AGCCTGGAGA AGAACGAGAG GGCGTGCATT GACTTCGCCA TCAGTGCCAA ACCTCTCACC 
CGCTACATGC CACAGAACAG GCACACCTTC CAGTACCGCG TCTGGCACTT CGTGGTGTCT 
CCGTCCTTTG AGTACACCAT CATGGCCATG ATCGCCCTGA ACACCGTCGT GCTGATGATG 
AAGTACTACT CTGCTCCCTG TACCTATGAA CTGGCCCTGA AGTACCTGAA TATTGCCTTC 
ACCATGGTGT TTTCCCTGGA ATGTGTCCTA AAGATCATCG CTTTTGGCTT CTTGAACTAC 
TTTCGAGACA CCTGGAACAT CTTTGACTTC ATCACTGTGA TTGGCAGTAT CACAGAAATC 
ATACTGACAG ACAGCAAGCT GGTAAACACC AGTGGTTTCA ACATGAGCTT TCTGAAGCTC 
TTCCGTGCCG CCCGCCTCAT CAAGCTTCTG CGGCAGGGCT ACACCATCCG TATTTTACTC 
TGGACCTTCG TGCAGTCCTT TAAGGCCCTC CCCTACGTCT GCCTTTTGAT TGCCATGCTT 
TTCTTCATCT ATGCCATCAT TGGGATGCAG GTATTTGGAA ACATAAAATT AGATGAGGAG 
AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GGTCCCTCAT GTTACTCTTC 
AGGAGTGCCA CGGGAGAGGC CTGGCAGGAG ATAATGCTGT CGTGCCTCGG GGAGAAAGGC 
TGCGAGCCCG ACACCACTGC GCCATCGGGG CAGAACGAGA ATGAGCGCTG TGGCACCGAT 
CTGGCCTATG TTTACTTTGT CTCCTTCATC TTCTTCTGCT CTTTCTTGAT GCTCAACTTG 
TTTGTGGCCG TCATCATGGA CAACTTCGAG TACCTGACTC GGGACTCCTC CATCTTAGGG 
CCCCACCACT TGGATGAGTT TGTCCGCGTC TGGGCAGAGT ACGACCGAGC TGCATGCGGC 
AGGATCCCGT ATAAGGATAT GTACAAATTA GTGCGGGTGA TCTCGCCGCC TCTGGGCCTG 
GGAGAGAACT GCCCCTACAG GGTGGCTTGC AAGAGGTTGG TCCTAATGAA TATGCCAGTA 
GCTGAGGACA TGACAGTCCA CTTCACCTCC ACACTGATGG CTCTGATCCG GACAGCCCTG 
GACATCAAAA TCGCCAAAGG TGGTGCAGAC AGGCAGCAGC TAGACTCGGA GCTGCAAAAG 
GAGACCCTGG CCATCTGGCC TCACCTGTCC CAGAAGATGC TAGACCTGCT TGTGCCCATG 
CCCAAAGCCT CTGACCTGAC TGTGGGCAAA ATCTATGCAG CAATGATGAT CATGGACTAC 
TATAAACAGA GTAAGGTGAA GAAACAGAGG CAGCAGCTGG AGGAACAGAA AAATGCACCC 
ATGTTCCAGC GAATGGAGCC CTCATCTCTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC 
CTGCCCTATC TCCAGCAGGA CCCCGTTTCA GGCCTGAGTG GCCGTAGTGG ATACCCTTCG 
ATGAGTCCAC TCTCCCCCCA GGAAATATTC CAATTGGCTT GTATGGATCC AGCCGATGAT 
GGACAGTTCC AGGAACATCA GTCTCTGGTG GTGACAGATA CTAGCTCCAT GAGACGTTCA 
TTTTCCACTA TTCGGGATAA GCGTTCAAAT TCCTCCTGGT TGGAGGAATT CTCCATGGAG 
AGAAGCAGTG AAAACACCTA CAAGTCACGT CGTCGGAGTT ACCACTCCTC CCTGAGACTG 
TCAGCTCATC GCCTGAATTC TGACTCAGGC CACAAGTCTG ACACCCACCG CTCAGGGGGT 
AGAGAGCGGG GACGATCGAA AGAGCGAAAG CATCTTCTCT CTCCTGATGT CTCCCGCTGC 
AACTCAGAGG AGCGAGGGAC CCAGGCCGAC TGGGAGTCCC CAGAGCGCCA ACAGTCCAGG 
TCTCCCAGTG AGGCCAGGTC ACAGACCCCC AACAGACAGG GCACGGGCTC CCTGAGCGAG 
AGCTCCATCC CCTCCATCTC TGACACCAGC ACCCCAAGAC GAAGTCGCCG GCAGCTCCCA 
CCCGTCCCAC CAAAGCCTCG GCCCCTCCTC TCCTACAGCT CCCTGATTCG ACACTCCGGG 
AGCATCTCCC CGCCGGCCGA CGGAAGCGAG AGGGGCTCCC CGCTGACCCC CCAGGCGCTG 
GAGAGCACGG AGGCCGGCCT GGCGGAGCCC TCGGGCCCCC GGCACGGGCA CGGGCAGGGG 
CACGGGCAGG GGCACGGGCA GCAGAGCCAG CACTGCTCCC CGCAGCGCTA CATCTCCGAG 
CCCTACCTGG CCCTGCACGA AGACTCCCAC GCGTCGGACT GTGGCGAGGA GGAGACGCTC 
ACCTTCGAGG CCGCCGTGGC CACCAGCCTG GGCCGCTCCA ACACCATCGG CTCGGCCCCG 
CCGCTGCGGC ACAGCTGGCA GATGCCCAAC GGGCACTATC GGCGGCGGCG GCGCGGGGGG 
CCGGGGCCGG GCGTGATGTG CGGGGCGGTC AGCGACCTCC TGAGTGACAC GGAAGAAGAC 
GACAAGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276642.1
CDS	6..7217
Translation	MRGPSPGRRLPEPAPCPAAAGSPEPWAAGRRRHVLALPEEEAWRPQQCSLLEADASDEVGMLPASPRAAASGVDNFFPVQEGEGAGWEGGAALDAGAGPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFTNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLGLGLEKCEEERIISRGGSLKGDGGDLSSALDNQRSPLSLGKREPPWLARPCHGNCDPTQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKELSSTSRSRSVSQERSLDEGVLTDGEKEREPRGGHAGKEPTIQEEDRGQDLRRTNSLMVPRGSGLAGALDEANTPLVLPHHDLEVGKDTVLTEQEPEGSSEQALLGDVQLDIGRVISQSEPDLSCVTANTDKATTDSTSVTVAIPDVGPLMDSTVIHISNKTDGEASPLKEAEIKEEEEEMEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIPYKDMYKLVRVISPPLGLGENCPYRVACKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEHQSLVVTDTSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERQQSRSPSEARSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHSGSISPPADGSERGSPLTPQALESTEAGLAEPSGPRHGHGQGHGQGHGQQSQHCSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGVMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_022420934.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022420934.1

ORF Insert Sequence:

ATGCGTGGAC CGAGCCCAGG CCGCAGGCTG CCCGAGCCCG CCCCCTGCCC CGCCGCGGCC 
GGGAGCCCTG AGCCCTGGGC CGCGGGCCGC CGCCGCCACG TCCTGGCCCT GCCTGAGGAG 
GAGGCATGGA GGCCCCAGCA GTGCTCCCTG CTCGAGGCCG ACGCCTCGGA CGAGGTGGGC 
ATGCTGCCGG CCTCCCCGCG CGCCGCCGCC TCCGGCGTCG ACAACTTCTT CCCGGTGCAG 
GAGGGCGAGG GCGCGGGCTG GGAGGGCGGT GCGGCCCTGG ACGCCGGCGC CGGCCCCTTC 
CTCCCGGTGA GCCCCGAGGT GATGAAGCGG CGGCGCGGGG GCCTCATCGA GCAGCGCGAC 
ATCATCAAGG CCCACGAGGC GCACAAGATG CAGAGCACCC CGCAGGCGCG GCGCAAGGAG 
TGGGAGATGG CTCGCTTCGG GGAGGCGGTG GTCGGCAGGC CGGGGTCGGG CGATGGAGAC 
TCGGACCAGA GCAGGAACCG GCAAGGAACC CCGGTGCCGG CCTCGGGGCC GGCGGCCGCC 
TACAAGCAGT CGAAAGCGCA GAGGGCGCGG ACTATGGCTT TGTACAACCC CATTCCCGTC 
CGGCAGAACT GTTTCACCGT CAACAGATCC CTGTTCATCT TCGGAGAAGA TAACATTGTC 
AGGAAATACG CCAAGAAGCT CATCGACTGG CCGCCGTTTG AGTACATGAT TTTGGCAACC 
ATCATTGCCA ACTGCATTGT CTTGGCCCTG GAACAGCATC TTCCTGAGGA TGACAAGACC 
CCGATGTCCC GCAGACTGGA GAAGACAGAG CCGTATTTCA TTGGGATCTT TTGCTTTGAA 
GCCGGTATCA AAATTGTGGC CCTGGGGTTC ATCTTTCATA AGGGCTCGTA CCTCCGCAAT 
GGCTGGAATG TCATGGACTT CATCGTGGTC CTCAGTGGCA TCCTGGCCAC TGCGGGGACT 
CACTTCAACA CACACGTGGA CCTGAGGACC CTCCGGGCTG TGCGTGTCCT GAGACCCCTG 
AAGCTCGTGT CAGGGATTCC TAGCCTGCAG ATCGTGTTGA AGTCTATCAT GAAGGCCATG 
GTGCCTCTTC TGCAAATTGG TCTCCTGCTC TTCTTTGCCA TCTTGATGTT TGCTATCATT 
GGCTTAGAGT TCTACAGTGG GAAGTTGCAC CGAGCATGTT TCACGAACAA TTCAGGTATT 
CTGGAAGGAT TTGACCCCCC TCACCCATGT GGTGTGCAGG GCTGCCCAGC TGGCTACGAA 
TGCCGGGACT GGATTGGCCC CAATGATGGG ATCACCCAGT TTGATAACAT CCTTTTTGCT 
GTGCTGACTG TCTTCCAATG CATCACCATG GAAGGATGGA CCACCGTGCT GTACAATACC 
AATGATGCCT TAGGAGCCAC CTGGAACTGG CTGTACTTCA TCCCCCTCAT CATCATTGGA 
TCCTTCTTTG TTCTCAACCT AGTTCTGGGA GTGCTTTCCG GGGAGTTTGC CAAAGAGAGA 
GAGAGAGTGG AGAACCGGAG AGCTTTCATG AAACTGCGGC GGCAGCAGCA GATTGAGCGG 
GAGCTGAACG GGTACCGCGC CTGGATAGAC AAAGCAGAGG AAGTCATGCT TGCTGAAGAA 
AATAAAAATG CTGGGACGTC AGCCTTAGAA GTGCTTCGAA GGGCAACCAT CAAAAGGAGC 
CGGACAGAGG CCATGACTCG AGACTCCAGT GATGAACACT GTGTCGATAT CTCCTCTGTG 
GGCACACCTC TCGCCCGAGC CAGCATCAAA AGTGCAAAGG TGGATGGGGC CTCCTATTTC 
CGGCACAAAG AAAGGCTTCT GCGCATCTCT ATCCGCCACA TGGTGAAATC CCAGGTGTTT 
TACTGGATTG TGTTGAGCCT TGTGGCTCTC AACACTGCCT GTGTGGCCAT CGTCCATCAT 
AACCAGCCCC AGTGGCTCAC CCATCTCCTC TACTATGCAG AATTTTTGTT TCTGGGACTG 
TTCCTCTTGG AGATGTCCCT GAAGATGTAT GGCATGGGAC CTCGACTTTA TTTTCACTCT 
TCATTCAACT GTTTTGATTT TGGGGTCACA GTGGGCAGCA TCTTTGAAGT GGTCTGGGCA 
ATCTTCAGAC CTGGCACATC TTTTGGAATC AGTGTCTTAC GGGCTCTCCG GCTTCTAAGA 
ATATTTAAAA TAACCAAGTA TTGGGCATCT CTGCGAAATT TGGTGGTCTC CTTGATGAGC 
TCTATGAAGT CTATCATCAG TTTGCTCTTC CTCCTCTTCC TCTTCATCGT TGTCTTTGCT 
CTCCTAGGAA TGCAGCTGTT TGGAGGCAGG TTTAACTTTA ATGATGGGAC TCCTTCAGCA 
AACTTTGATA CCTTCCCTGC AGCCATCATG ACTGTATTCC AGATCCTAAC AGGTGAGGAC 
TGGAATGAGG TGATGTACAA TGGGATCCGT TCCCAGGGTG GAGTTAGCTC AGGCATGTGG 
TCAGCTATCT ATTTCATTGT GCTCACCTTG TTTGGAAACT ACACACTGCT GAATGTGTTC 
TTGGCCATTG CTGTGGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA TGAACAAGAA 
GAAGAGGAGG CTTTTAACCA GAAACATGCA TTGCAGAAGG CCAAGGAGGT CAGCCCGATG 
TCTGCACCCA ACATGCCTTC TATCGAGAGG GAGCGGAGGC GCCGGCACCA CATGTCGGTG 
TGGGAGCAGC GCACCAGCCA GCTGAGAAGG CACATGCAGA TGTCCAGCCA GGAGGCCCTC 
AATAAAGAGG AGGCCCCACC GATGAACCCC CTCAACCCCC TCAACCCGCT GAGCCCCCTC 
AACCCGCTCA ACGCCCACCC CAGCCTCTAC CGGAGACCTA GGCCTATTGA GGGCCTGGGC 
CTGGGCCTGG AGAAGTGTGA GGAGGAGCGC ATCATCAGCC GCGGGGGGTC CCTCAAGGGG 
GACGGAGGGG ACCTGTCGAG TGCCCTGGAC AACCAGAGGA GCCCTTTGTC CCTGGGCAAA 
CGGGAGCCAC CATGGCTGGC CAGGCCCTGT CATGGGAACT GTGATCCGAC CCAGCAGGAG 
GCTGGGGGAG GGGAGACCGT GGTGACCTTC GAGGACCGGG CCAGGCACAG GCAGAGCCAG 
CGGCGCAGCC GGCACCGTCG TGTCAGGACG GAAGGCAAGG AGCTCTCCTC CACCTCCCGG 
AGCAGGTCTG TCAGCCAGGA GCGGAGTCTG GATGAGGGGG TGCTCACCGA CGGGGAGAAG 
GAGCGAGAGC CCAGGGGCGG CCATGCAGGC AAAGAACCCA CCATCCAAGA AGAAGACAGA 
GGCCAAGACT TAAGGAGGAC GAACAGTCTG ATGGTGCCCA GAGGCTCTGG GCTGGCAGGA 
GCCCTTGATG AGGCCAACAC ACCCCTAGTC CTGCCCCACC ACGATCTGGA AGTGGGGAAG 
GACACGGTGC TGACGGAGCA GGAGCCGGAG GGCAGCAGCG AGCAGGCCCT GCTGGGGGAT 
GTGCAGTTGG ACATTGGCAG GGTCATCAGC CAGAGTGAGC CTGACCTCTC CTGCGTCACA 
GCCAACACAG ACAAGGCCAC CACCGACAGC ACCAGCGTCA CCGTCGCCAT CCCTGATGTG 
GGCCCCTTGA TGGACTCAAC TGTGATTCAC ATTAGCAACA AAACTGATGG AGAAGCCAGT 
CCCTTGAAGG AGGCTGAGAT CAAAGAGGAG GAGGAGGAAA TGGAGAAGAA GCAGCAGAAG 
AAGGAGAAGC GTGAGACGGG CAAAGCCATG GTGCCCCACA GCTCTATGTT CATCTTCAGC 
ACCACCAACC CGATTCGCAG GGCCTGCCAC TACATCGTGA ACCTGCGCTA CTTTGAGATG 
TGCATTCTCC TGGTGATCGC CGCCAGCAGC ATTGCCCTGG CAGCAGAGGA CCCCGTCCTG 
ACCAACTCGG AGCGCAACAA AGTCCTGAGG TATTTTGACT ATGTTTTCAC TGGCGTGTTC 
ACCTTTGAGA TGGTTATAAA GATGATAGAC CAGGGCTTGA TCCTACAGGA TGGGTCCTAC 
TTCCGAGACC TGTGGAACAT CCTGGACTTT GTGGTGGTGG TTGGTGCATT GGTGGCCTTT 
GCTTTGGCGA ACGCTTTGGG AACAAACAAG GGGCGAGACA TCAAGACCAT CAAATCCCTG 
AGGGTGCTCC GAGTTCTGAG GCCACTGAAG ACCATCAAGC GCTTGCCCAA ACTCAAGGCT 
GTCTTTGACT GTGTGGTGAC CTCCTTGAAG AATGTCTTCA ACATACTCAT CGTCTATAAG 
CTCTTCATGT TCATCTTTGC TGTCATTGCG GTTCAGCTCT TCAAGGGAAA ATTCTTTTAT 
TGCACAGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTACGT AGATCATGAA 
AAAAACAAGA TGGAGGTGAA AGGCCGGGAA TGGAAGCGCC ATGAATTCCA CTACGACAAC 
ATTATCTGGG CCCTGCTGAC CCTCTTCACC GTCTCCACGG GGGAAGGATG GCCTCAAGTC 
TTACAGCATT CTGTCGATGT GACAGAGGAA GACCGAGGAC CAAGCCGCAG CAACCGCATG 
GAGATGTCCA TCTTCTATGT GGTCTACTTT GTGGTCTTCC CTTTCTTCTT TGTCAATATC 
TTTGTGGCTC TGATCATCAT CACCTTCCAG GAACAAGGGG ACAAGATGAT GGAGGAATGC 
AGCCTGGAGA AGAACGAGAG GGCGTGCATT GACTTCGCCA TCAGTGCCAA ACCTCTCACC 
CGCTACATGC CACAGAACAG GCACACCTTC CAGTACCGCG TCTGGCACTT CGTGGTGTCT 
CCGTCCTTTG AGTACACCAT CATGGCCATG ATCGCCCTGA ACACCGTCGT GCTGATGATG 
AAGTACTACT CTGCTCCCTG TACCTATGAA CTGGCCCTGA AGTACCTGAA TATTGCCTTC 
ACCATGGTGT TTTCCCTGGA ATGTGTCCTA AAGATCATCG CTTTTGGCTT CTTGAACTAC 
TTTCGAGACA CCTGGAACAT CTTTGACTTC ATCACTGTGA TTGGCAGTAT CACAGAAATC 
ATACTGACAG ACAGCAAGCT GGTAAACACC AGTGGTTTCA ACATGAGCTT TCTGAAGCTC 
TTCCGTGCCG CCCGCCTCAT CAAGCTTCTG CGGCAGGGCT ACACCATCCG TATTTTACTC 
TGGACCTTCG TGCAGTCCTT TAAGGCCCTC CCCTACGTCT GCCTTTTGAT TGCCATGCTT 
TTCTTCATCT ATGCCATCAT TGGGATGCAG GTATTTGGAA ACATAAAATT AGATGAGGAG 
AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GGTCCCTCAT GTTACTCTTC 
AGGAGTGCCA CGGGAGAGGC CTGGCAGGAG ATAATGCTGT CGTGCCTCGG GGAGAAAGGC 
TGCGAGCCCG ACACCACTGC GCCATCGGGG CAGAACGAGA ATGAGCGCTG TGGCACCGAT 
CTGGCCTATG TTTACTTTGT CTCCTTCATC TTCTTCTGCT CTTTCTTGAT GCTCAACTTG 
TTTGTGGCCG TCATCATGGA CAACTTCGAG TACCTGACTC GGGACTCCTC CATCTTAGGG 
CCCCACCACT TGGATGAGTT TGTCCGCGTC TGGGCAGAGT ACGACCGAGC TGCATGCGGC 
AGGATCCCGT ATAAGGATAT GTACAAATTA GTGCGGGTGA TCTCGCCGCC TCTGGGCCTG 
GGAGAGAACT GCCCCTACAG GGTGGCTTGC AAGAGGTTGG TCCTAATGAA TATGCCAGTA 
GCTGAGGACA TGACAGTCCA CTTCACCTCC ACACTGATGG CTCTGATCCG GACAGCCCTG 
GACATCAAAA TCGCCAAAGG TGGTGCAGAC AGGCAGCAGC TAGACTCGGA GCTGCAAAAG 
GAGACCCTGG CCATCTGGCC TCACCTGTCC CAGAAGATGC TAGACCTGCT TGTGCCCATG 
CCCAAAGCCT CTGACCTGAC TGTGGGCAAA ATCTATGCAG CAATGATGAT CATGGACTAC 
TATAAACAGA GTAAGGTGAA GAAACAGAGG CAGCAGCTGG AGGAACAGAA AAATGCACCC 
ATGTTCCAGC GAATGGAGCC CTCATCTCTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC 
CTGCCCTATC TCCAGCAGGA CCCCGTTTCA GGCCTGAGTG GCCGTAGTGG ATACCCTTCG 
ATGAGTCCAC TCTCCCCCCA GGAAATATTC CAATTGGCTT GTATGGATCC AGCCGATGAT 
GGACAGTTCC AGGAACATCA GTCTCTGGTG GTGACAGATA CTAGCTCCAT GAGACGTTCA 
TTTTCCACTA TTCGGGATAA GCGTTCAAAT TCCTCCTGGT TGGAGGAATT CTCCATGGAG 
AGAAGCAGTG AAAACACCTA CAAGTCACGT CGTCGGAGTT ACCACTCCTC CCTGAGACTG 
TCAGCTCATC GCCTGAATTC TGACTCAGGC CACAAGTCTG ACACCCACCG CTCAGGGGGT 
AGAGAGCGGG GACGATCGAA AGAGCGAAAG CATCTTCTCT CTCCTGATGT CTCCCGCTGC 
AACTCAGAGG AGCGAGGGAC CCAGGCCGAC TGGGAGTCCC CAGAGCGCCA ACAGTCCAGG 
TCTCCCAGTG AGGCCAGGTC ACAGACCCCC AACAGACAGG GCACGGGCTC CCTGAGCGAG 
AGCTCCATCC CCTCCATCTC TGACACCAGC ACCCCAAGAC GAAGTCGCCG GCAGCTCCCA 
CCCGTCCCAC CAAAGCCTCG GCCCCTCCTC TCCTACAGCT CCCTGATTCG ACACTCCGGG 
AGCATCTCCC CGCCGGCCGA CGGAAGCGAG AGGGGCTCCC CGCTGACCCC CCAGGCGCTG 
GAGAGCACGG AGGCCGGCCT GGCGGAGCCC TCGGGCCCCC GGCACGGGCA CGGGCAGGGG 
CACGGGCAGG GGCACGGGCA GCAGAGCCAG CACTGCTCCC CGCAGCGCTA CATCTCCGAG 
CCCTACCTGG CCCTGCACGA AGACTCCCAC GCGTCGGACT GTGGCGAGGA GGAGACGCTC 
ACCTTCGAGG CCGCCGTGGC CACCAGCCTG GGCCGCTCCA ACACCATCGG CTCGGCCCCG 
CCGCTGCGGC ACAGCTGGCA GATGCCCAAC GGGCACTATC GGCGGCGGCG GCGCGGGGGG 
CCGGGGCCGG GCGTGATGTG CGGGGCGGTC AGCGACCTCC TGAGTGACAC GGAAGAAGAC 
GACAAGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_022420935.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022276643.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCe289501
Clone ID Related Accession (Same CDS sequence)	XM_022420935.1
Accession Version	XM_022420935.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCGGTG CCGGCCTCGG GGCCGGCGGC CGCCTACAAG 
CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TACGCCAAGA AGCTCATCGA CTGGCCGCCG TTTGAGTACA TGATTTTGGC AACCATCATT 
GCCAACTGCA TTGTCTTGGC CCTGGAACAG CATCTTCCTG AGGATGACAA GACCCCGATG 
TCCCGCAGAC TGGAGAAGAC AGAGCCGTAT TTCATTGGGA TCTTTTGCTT TGAAGCCGGT 
ATCAAAATTG TGGCCCTGGG GTTCATCTTT CATAAGGGCT CGTACCTCCG CAATGGCTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCGGG GACTCACTTC 
AACACACACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGAGACC CCTGAAGCTC 
GTGTCAGGGA TTCCTAGCCT GCAGATCGTG TTGAAGTCTA TCATGAAGGC CATGGTGCCT 
CTTCTGCAAA TTGGTCTCCT GCTCTTCTTT GCCATCTTGA TGTTTGCTAT CATTGGCTTA 
GAGTTCTACA GTGGGAAGTT GCACCGAGCA TGTTTCACGA ACAATTCAGG TATTCTGGAA 
GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGCTA CGAATGCCGG 
GACTGGATTG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG 
ACTGTCTTCC AATGCATCAC CATGGAAGGA TGGACCACCG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA CTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTAGTTCT GGGAGTGCTT TCCGGGGAGT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GGAGAGCTTT CATGAAACTG CGGCGGCAGC AGCAGATTGA GCGGGAGCTG 
AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAATAAA 
AATGCTGGGA CGTCAGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAAAG GAGCCGGACA 
GAGGCCATGA CTCGAGACTC CAGTGATGAA CACTGTGTCG ATATCTCCTC TGTGGGCACA 
CCTCTCGCCC GAGCCAGCAT CAAAAGTGCA AAGGTGGATG GGGCCTCCTA TTTCCGGCAC 
AAAGAAAGGC TTCTGCGCAT CTCTATCCGC CACATGGTGA AATCCCAGGT GTTTTACTGG 
ATTGTGTTGA GCCTTGTGGC TCTCAACACT GCCTGTGTGG CCATCGTCCA TCATAACCAG 
CCCCAGTGGC TCACCCATCT CCTCTACTAT GCAGAATTTT TGTTTCTGGG ACTGTTCCTC 
TTGGAGATGT CCCTGAAGAT GTATGGCATG GGACCTCGAC TTTATTTTCA CTCTTCATTC 
AACTGTTTTG ATTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTGGTCTG GGCAATCTTC 
AGACCTGGCA CATCTTTTGG AATCAGTGTC TTACGGGCTC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC ATCTCTGCGA AATTTGGTGG TCTCCTTGAT GAGCTCTATG 
AAGTCTATCA TCAGTTTGCT CTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA 
GGAATGCAGC TGTTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC AGCAAACTTT 
GATACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TAACAGGTGA GGACTGGAAT 
GAGGTGATGT ACAATGGGAT CCGTTCCCAG GGTGGAGTTA GCTCAGGCAT GTGGTCAGCT 
ATCTATTTCA TTGTGCTCAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC 
ATTGCTGTGG ACAATCTGGC CAATGCCCAA GAGCTGACCA AGGATGAACA AGAAGAAGAG 
GAGGCTTTTA ACCAGAAACA TGCATTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCTATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG 
CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCACC ACATGTCGGT GTGGGAGCAG 
CGCACCAGCC AGCTGAGAAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAATAAAGAG 
GAGGCCCCAC CGATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC 
AACGCCCACC CCAGCCTCTA CCGGAGACCT AGGCCTATTG AGGGCCTGGG CCTGGGCCTG 
GAGAAGTGTG AGGAGGAGCG CATCATCAGC CGCGGGGGGT CCCTCAAGGG GGACGGAGGG 
GACCTGTCGA GTGCCCTGGA CAACCAGAGG AGCCCTTTGT CCCTGGGCAA ACGGGAGCCA 
CCATGGCTGG CCAGGCCCTG TCATGGGAAC TGTGATCCGA CCCAGCAGGA GGCTGGGGGA 
GGGGAGACCG TGGTGACCTT CGAGGACCGG GCCAGGCACA GGCAGAGCCA GCGGCGCAGC 
CGGCACCGTC GTGTCAGGAC GGAAGGCAAG GAGCTCTCCT CCACCTCCCG GAGCAGGTCT 
GTCAGCCAGG AGCGGAGTCT GGATGAGGGG GTGCTCACCG ACGGGGAGAA GGAGCGAGAG 
CCCAGGGGCG GCCATGCAGG CAAAGAACCC ACCATCCAAG AAGAAGACAG AGGCCAAGAC 
TTAAGGAGGA CGAACAGTCT GATGGTGCCC AGAGGCTCTG GGCTGGCAGG AGCCCTTGAT 
GAGGCCAACA CACCCCTAGT CCTGCCCCAC CACGATCTGG AAGTGGGGAA GGACACGGTG 
CTGACGGAGC AGGAGCCGGA GGGCAGCAGC GAGCAGGCCC TGCTGGGGGA TGTGCAGTTG 
GACATTGGCA GGGTCATCAG CCAGAGTGAG CCTGACCTCT CCTGCGTCAC AGCCAACACA 
GACAAGGCCA CCACCGACAG CACCAGCGTC ACCGTCGCCA TCCCTGATGT GGGCCCCTTG 
ATGGACTCAA CTGTGATTCA CATTAGCAAC AAAACTGATG GAGAAGCCAG TCCCTTGAAG 
GAGGCTGAGA TCAAAGAGGA GGAGGAGGAA ATGGAGAAGA AGCAGCAGAA GAAGGAGAAG 
CGTGAGACGG GCAAAGCCAT GGTGCCCCAC AGCTCTATGT TCATCTTCAG CACCACCAAC 
CCGATTCGCA GGGCCTGCCA CTACATCGTG AACCTGCGCT ACTTTGAGAT GTGCATTCTC 
CTGGTGATCG CCGCCAGCAG CATTGCCCTG GCAGCAGAGG ACCCCGTCCT GACCAACTCG 
GAGCGCAACA AAGTCCTGAG GTATTTTGAC TATGTTTTCA CTGGCGTGTT CACCTTTGAG 
ATGGTTATAA AGATGATAGA CCAGGGCTTG ATCCTACAGG ATGGGTCCTA CTTCCGAGAC 
CTGTGGAACA TCCTGGACTT TGTGGTGGTG GTTGGTGCAT TGGTGGCCTT TGCTTTGGCG 
AACGCTTTGG GAACAAACAA GGGGCGAGAC ATCAAGACCA TCAAATCCCT GAGGGTGCTC 
CGAGTTCTGA GGCCACTGAA GACCATCAAG CGCTTGCCCA AACTCAAGGC TGTCTTTGAC 
TGTGTGGTGA CCTCCTTGAA GAATGTCTTC AACATACTCA TCGTCTATAA GCTCTTCATG 
TTCATCTTTG CTGTCATTGC GGTTCAGCTC TTCAAGGGAA AATTCTTTTA TTGCACAGAC 
AGTTCCAAGG ACACAGAGAA GGAGTGCATA GGCAACTACG TAGATCATGA AAAAAACAAG 
ATGGAGGTGA AAGGCCGGGA ATGGAAGCGC CATGAATTCC ACTACGACAA CATTATCTGG 
GCCCTGCTGA CCCTCTTCAC CGTCTCCACG GGGGAAGGAT GGCCTCAAGT CTTACAGCAT 
TCTGTCGATG TGACAGAGGA AGACCGAGGA CCAAGCCGCA GCAACCGCAT GGAGATGTCC 
ATCTTCTATG TGGTCTACTT TGTGGTCTTC CCTTTCTTCT TTGTCAATAT CTTTGTGGCT 
CTGATCATCA TCACCTTCCA GGAACAAGGG GACAAGATGA TGGAGGAATG CAGCCTGGAG 
AAGAACGAGA GGGCGTGCAT TGACTTCGCC ATCAGTGCCA AACCTCTCAC CCGCTACATG 
CCACAGAACA GGCACACCTT CCAGTACCGC GTCTGGCACT TCGTGGTGTC TCCGTCCTTT 
GAGTACACCA TCATGGCCAT GATCGCCCTG AACACCGTCG TGCTGATGAT GAAGTACTAC 
TCTGCTCCCT GTACCTATGA ACTGGCCCTG AAGTACCTGA ATATTGCCTT CACCATGGTG 
TTTTCCCTGG AATGTGTCCT AAAGATCATC GCTTTTGGCT TCTTGAACTA CTTTCGAGAC 
ACCTGGAACA TCTTTGACTT CATCACTGTG ATTGGCAGTA TCACAGAAAT CATACTGACA 
GACAGCAAGC TGGTAAACAC CAGTGGTTTC AACATGAGCT TTCTGAAGCT CTTCCGTGCC 
GCCCGCCTCA TCAAGCTTCT GCGGCAGGGC TACACCATCC GTATTTTACT CTGGACCTTC 
GTGCAGTCCT TTAAGGCCCT CCCCTACGTC TGCCTTTTGA TTGCCATGCT TTTCTTCATC 
TATGCCATCA TTGGGATGCA GGTATTTGGA AACATAAAAT TAGATGAGGA GAGTCACATC 
AACCGGCACA ACAACTTCCG GAGTTTCTTT GGGTCCCTCA TGTTACTCTT CAGGAGTGCC 
ACGGGAGAGG CCTGGCAGGA GATAATGCTG TCGTGCCTCG GGGAGAAAGG CTGCGAGCCC 
GACACCACTG CGCCATCGGG GCAGAACGAG AATGAGCGCT GTGGCACCGA TCTGGCCTAT 
GTTTACTTTG TCTCCTTCAT CTTCTTCTGC TCTTTCTTGA TGCTCAACTT GTTTGTGGCC 
GTCATCATGG ACAACTTCGA GTACCTGACT CGGGACTCCT CCATCTTAGG GCCCCACCAC 
TTGGATGAGT TTGTCCGCGT CTGGGCAGAG TACGACCGAG CTGCATGTGG CCGCATCCAT 
TATACTGAGA TGTATGAAAT GCTGACTCTC ATGTCACCAC CGCTAGGCCT CGGCAAGAGA 
TGTCCCTCCA AAGTGGCATA TAAGAGGTTG GTCCTAATGA ATATGCCAGT AGCTGAGGAC 
ATGACAGTCC ACTTCACCTC CACACTGATG GCTCTGATCC GGACAGCCCT GGACATCAAA 
ATCGCCAAAG GTGGTGCAGA CAGGCAGCAG CTAGACTCGG AGCTGCAAAA GGAGACCCTG 
GCCATCTGGC CTCACCTGTC CCAGAAGATG CTAGACCTGC TTGTGCCCAT GCCCAAAGCC 
TCTGACCTGA CTGTGGGCAA AATCTATGCA GCAATGATGA TCATGGACTA CTATAAACAG 
AGTAAGGTGA AGAAACAGAG GCAGCAGCTG GAGGAACAGA AAAATGCACC CATGTTCCAG 
CGAATGGAGC CCTCATCTCT GCCTCAGGAG ATCATTGCTA ATGCCAAAGC CCTGCCCTAT 
CTCCAGCAGG ACCCCGTTTC AGGCCTGAGT GGCCGTAGTG GATACCCTTC GATGAGTCCA 
CTCTCCCCCC AGGAAATATT CCAATTGGCT TGTATGGATC CAGCCGATGA TGGACAGTTC 
CAGGAACATC AGTCTCTGGA GCCAGAGGTT AGTGAATTAA AAAGCGTGCA GTCCTCTAAC 
CATGGCATCT ACCTTCCTTC GGACACCCAG GAGCATACGG GATCTGGGAG GGCATCTTCT 
ATGCCACGCC TGACTGTGGA TCCCCAGGTG GTGACAGATA CTAGCTCCAT GAGACGTTCA 
TTTTCCACTA TTCGGGATAA GCGTTCAAAT TCCTCCTGGT TGGAGGAATT CTCCATGGAG 
AGAAGCAGTG AAAACACCTA CAAGTCACGT CGTCGGAGTT ACCACTCCTC CCTGAGACTG 
TCAGCTCATC GCCTGAATTC TGACTCAGGC CACAAGTCTG ACACCCACCG CTCAGGGGGT 
AGAGAGCGGG GACGATCGAA AGAGCGAAAG CATCTTCTCT CTCCTGATGT CTCCCGCTGC 
AACTCAGAGG AGCGAGGGAC CCAGGCCGAC TGGGAGTCCC CAGAGCGCCA ACAGTCCAGG 
TCTCCCAGTG AGGCCAGGTC ACAGACCCCC AACAGACAGG GCACGGGCTC CCTGAGCGAG 
AGCTCCATCC CCTCCATCTC TGACACCAGC ACCCCAAGAC GAAGTCGCCG GCAGCTCCCA 
CCCGTCCCAC CAAAGCCTCG GCCCCTCCTC TCCTACAGCT CCCTGATTCG ACACTCCGGG 
AGCATCTCCC CGCCGGCCGA CGGAAGCGAG AGGGGCTCCC CGCTGACCCC CCAGGCGCTG 
GAGAGCACGG AGGCCGGCCT GGCGGAGCCC TCGGGCCCCC GGCACGGGCA CGGGCAGGGG 
CACGGGCAGG GGCACGGGCA GCAGAGCCAG CACTGCTCCC CGCAGCGCTA CATCTCCGAG 
CCCTACCTGG CCCTGCACGA AGACTCCCAC GCGTCGGACT GTGGCGAGGA GGAGACGCTC 
ACCTTCGAGG CCGCCGTGGC CACCAGCCTG GGCCGCTCCA ACACCATCGG CTCGGCCCCG 
CCGCTGCGGC ACAGCTGGCA GATGCCCAAC GGGCACTATC GGCGGCGGCG GCGCGGGGGG 
CCGGGGCCGG GCGTGATGTG CGGGGCGGTC AGCGACCTCC TGAGTGACAC GGAAGAAGAC 
GACAAGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276643.1
CDS	131..7102
Translation	MARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFTNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLGLGLEKCEEERIISRGGSLKGDGGDLSSALDNQRSPLSLGKREPPWLARPCHGNCDPTQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKELSSTSRSRSVSQERSLDEGVLTDGEKEREPRGGHAGKEPTIQEEDRGQDLRRTNSLMVPRGSGLAGALDEANTPLVLPHHDLEVGKDTVLTEQEPEGSSEQALLGDVQLDIGRVISQSEPDLSCVTANTDKATTDSTSVTVAIPDVGPLMDSTVIHISNKTDGEASPLKEAEIKEEEEEMEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEHQSLEPEVSELKSVQSSNHGIYLPSDTQEHTGSGRASSMPRLTVDPQVVTDTSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERQQSRSPSEARSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHSGSISPPADGSERGSPLTPQALESTEAGLAEPSGPRHGHGQGHGQGHGQQSQHCSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGVMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_022420935.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022420935.1

ORF Insert Sequence:

ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCGGTG CCGGCCTCGG GGCCGGCGGC CGCCTACAAG 
CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TACGCCAAGA AGCTCATCGA CTGGCCGCCG TTTGAGTACA TGATTTTGGC AACCATCATT 
GCCAACTGCA TTGTCTTGGC CCTGGAACAG CATCTTCCTG AGGATGACAA GACCCCGATG 
TCCCGCAGAC TGGAGAAGAC AGAGCCGTAT TTCATTGGGA TCTTTTGCTT TGAAGCCGGT 
ATCAAAATTG TGGCCCTGGG GTTCATCTTT CATAAGGGCT CGTACCTCCG CAATGGCTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCGGG GACTCACTTC 
AACACACACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGAGACC CCTGAAGCTC 
GTGTCAGGGA TTCCTAGCCT GCAGATCGTG TTGAAGTCTA TCATGAAGGC CATGGTGCCT 
CTTCTGCAAA TTGGTCTCCT GCTCTTCTTT GCCATCTTGA TGTTTGCTAT CATTGGCTTA 
GAGTTCTACA GTGGGAAGTT GCACCGAGCA TGTTTCACGA ACAATTCAGG TATTCTGGAA 
GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGCTA CGAATGCCGG 
GACTGGATTG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG 
ACTGTCTTCC AATGCATCAC CATGGAAGGA TGGACCACCG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA CTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTAGTTCT GGGAGTGCTT TCCGGGGAGT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GGAGAGCTTT CATGAAACTG CGGCGGCAGC AGCAGATTGA GCGGGAGCTG 
AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAATAAA 
AATGCTGGGA CGTCAGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAAAG GAGCCGGACA 
GAGGCCATGA CTCGAGACTC CAGTGATGAA CACTGTGTCG ATATCTCCTC TGTGGGCACA 
CCTCTCGCCC GAGCCAGCAT CAAAAGTGCA AAGGTGGATG GGGCCTCCTA TTTCCGGCAC 
AAAGAAAGGC TTCTGCGCAT CTCTATCCGC CACATGGTGA AATCCCAGGT GTTTTACTGG 
ATTGTGTTGA GCCTTGTGGC TCTCAACACT GCCTGTGTGG CCATCGTCCA TCATAACCAG 
CCCCAGTGGC TCACCCATCT CCTCTACTAT GCAGAATTTT TGTTTCTGGG ACTGTTCCTC 
TTGGAGATGT CCCTGAAGAT GTATGGCATG GGACCTCGAC TTTATTTTCA CTCTTCATTC 
AACTGTTTTG ATTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTGGTCTG GGCAATCTTC 
AGACCTGGCA CATCTTTTGG AATCAGTGTC TTACGGGCTC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC ATCTCTGCGA AATTTGGTGG TCTCCTTGAT GAGCTCTATG 
AAGTCTATCA TCAGTTTGCT CTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA 
GGAATGCAGC TGTTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC AGCAAACTTT 
GATACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TAACAGGTGA GGACTGGAAT 
GAGGTGATGT ACAATGGGAT CCGTTCCCAG GGTGGAGTTA GCTCAGGCAT GTGGTCAGCT 
ATCTATTTCA TTGTGCTCAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC 
ATTGCTGTGG ACAATCTGGC CAATGCCCAA GAGCTGACCA AGGATGAACA AGAAGAAGAG 
GAGGCTTTTA ACCAGAAACA TGCATTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCTATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG 
CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCACC ACATGTCGGT GTGGGAGCAG 
CGCACCAGCC AGCTGAGAAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAATAAAGAG 
GAGGCCCCAC CGATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC 
AACGCCCACC CCAGCCTCTA CCGGAGACCT AGGCCTATTG AGGGCCTGGG CCTGGGCCTG 
GAGAAGTGTG AGGAGGAGCG CATCATCAGC CGCGGGGGGT CCCTCAAGGG GGACGGAGGG 
GACCTGTCGA GTGCCCTGGA CAACCAGAGG AGCCCTTTGT CCCTGGGCAA ACGGGAGCCA 
CCATGGCTGG CCAGGCCCTG TCATGGGAAC TGTGATCCGA CCCAGCAGGA GGCTGGGGGA 
GGGGAGACCG TGGTGACCTT CGAGGACCGG GCCAGGCACA GGCAGAGCCA GCGGCGCAGC 
CGGCACCGTC GTGTCAGGAC GGAAGGCAAG GAGCTCTCCT CCACCTCCCG GAGCAGGTCT 
GTCAGCCAGG AGCGGAGTCT GGATGAGGGG GTGCTCACCG ACGGGGAGAA GGAGCGAGAG 
CCCAGGGGCG GCCATGCAGG CAAAGAACCC ACCATCCAAG AAGAAGACAG AGGCCAAGAC 
TTAAGGAGGA CGAACAGTCT GATGGTGCCC AGAGGCTCTG GGCTGGCAGG AGCCCTTGAT 
GAGGCCAACA CACCCCTAGT CCTGCCCCAC CACGATCTGG AAGTGGGGAA GGACACGGTG 
CTGACGGAGC AGGAGCCGGA GGGCAGCAGC GAGCAGGCCC TGCTGGGGGA TGTGCAGTTG 
GACATTGGCA GGGTCATCAG CCAGAGTGAG CCTGACCTCT CCTGCGTCAC AGCCAACACA 
GACAAGGCCA CCACCGACAG CACCAGCGTC ACCGTCGCCA TCCCTGATGT GGGCCCCTTG 
ATGGACTCAA CTGTGATTCA CATTAGCAAC AAAACTGATG GAGAAGCCAG TCCCTTGAAG 
GAGGCTGAGA TCAAAGAGGA GGAGGAGGAA ATGGAGAAGA AGCAGCAGAA GAAGGAGAAG 
CGTGAGACGG GCAAAGCCAT GGTGCCCCAC AGCTCTATGT TCATCTTCAG CACCACCAAC 
CCGATTCGCA GGGCCTGCCA CTACATCGTG AACCTGCGCT ACTTTGAGAT GTGCATTCTC 
CTGGTGATCG CCGCCAGCAG CATTGCCCTG GCAGCAGAGG ACCCCGTCCT GACCAACTCG 
GAGCGCAACA AAGTCCTGAG GTATTTTGAC TATGTTTTCA CTGGCGTGTT CACCTTTGAG 
ATGGTTATAA AGATGATAGA CCAGGGCTTG ATCCTACAGG ATGGGTCCTA CTTCCGAGAC 
CTGTGGAACA TCCTGGACTT TGTGGTGGTG GTTGGTGCAT TGGTGGCCTT TGCTTTGGCG 
AACGCTTTGG GAACAAACAA GGGGCGAGAC ATCAAGACCA TCAAATCCCT GAGGGTGCTC 
CGAGTTCTGA GGCCACTGAA GACCATCAAG CGCTTGCCCA AACTCAAGGC TGTCTTTGAC 
TGTGTGGTGA CCTCCTTGAA GAATGTCTTC AACATACTCA TCGTCTATAA GCTCTTCATG 
TTCATCTTTG CTGTCATTGC GGTTCAGCTC TTCAAGGGAA AATTCTTTTA TTGCACAGAC 
AGTTCCAAGG ACACAGAGAA GGAGTGCATA GGCAACTACG TAGATCATGA AAAAAACAAG 
ATGGAGGTGA AAGGCCGGGA ATGGAAGCGC CATGAATTCC ACTACGACAA CATTATCTGG 
GCCCTGCTGA CCCTCTTCAC CGTCTCCACG GGGGAAGGAT GGCCTCAAGT CTTACAGCAT 
TCTGTCGATG TGACAGAGGA AGACCGAGGA CCAAGCCGCA GCAACCGCAT GGAGATGTCC 
ATCTTCTATG TGGTCTACTT TGTGGTCTTC CCTTTCTTCT TTGTCAATAT CTTTGTGGCT 
CTGATCATCA TCACCTTCCA GGAACAAGGG GACAAGATGA TGGAGGAATG CAGCCTGGAG 
AAGAACGAGA GGGCGTGCAT TGACTTCGCC ATCAGTGCCA AACCTCTCAC CCGCTACATG 
CCACAGAACA GGCACACCTT CCAGTACCGC GTCTGGCACT TCGTGGTGTC TCCGTCCTTT 
GAGTACACCA TCATGGCCAT GATCGCCCTG AACACCGTCG TGCTGATGAT GAAGTACTAC 
TCTGCTCCCT GTACCTATGA ACTGGCCCTG AAGTACCTGA ATATTGCCTT CACCATGGTG 
TTTTCCCTGG AATGTGTCCT AAAGATCATC GCTTTTGGCT TCTTGAACTA CTTTCGAGAC 
ACCTGGAACA TCTTTGACTT CATCACTGTG ATTGGCAGTA TCACAGAAAT CATACTGACA 
GACAGCAAGC TGGTAAACAC CAGTGGTTTC AACATGAGCT TTCTGAAGCT CTTCCGTGCC 
GCCCGCCTCA TCAAGCTTCT GCGGCAGGGC TACACCATCC GTATTTTACT CTGGACCTTC 
GTGCAGTCCT TTAAGGCCCT CCCCTACGTC TGCCTTTTGA TTGCCATGCT TTTCTTCATC 
TATGCCATCA TTGGGATGCA GGTATTTGGA AACATAAAAT TAGATGAGGA GAGTCACATC 
AACCGGCACA ACAACTTCCG GAGTTTCTTT GGGTCCCTCA TGTTACTCTT CAGGAGTGCC 
ACGGGAGAGG CCTGGCAGGA GATAATGCTG TCGTGCCTCG GGGAGAAAGG CTGCGAGCCC 
GACACCACTG CGCCATCGGG GCAGAACGAG AATGAGCGCT GTGGCACCGA TCTGGCCTAT 
GTTTACTTTG TCTCCTTCAT CTTCTTCTGC TCTTTCTTGA TGCTCAACTT GTTTGTGGCC 
GTCATCATGG ACAACTTCGA GTACCTGACT CGGGACTCCT CCATCTTAGG GCCCCACCAC 
TTGGATGAGT TTGTCCGCGT CTGGGCAGAG TACGACCGAG CTGCATGTGG CCGCATCCAT 
TATACTGAGA TGTATGAAAT GCTGACTCTC ATGTCACCAC CGCTAGGCCT CGGCAAGAGA 
TGTCCCTCCA AAGTGGCATA TAAGAGGTTG GTCCTAATGA ATATGCCAGT AGCTGAGGAC 
ATGACAGTCC ACTTCACCTC CACACTGATG GCTCTGATCC GGACAGCCCT GGACATCAAA 
ATCGCCAAAG GTGGTGCAGA CAGGCAGCAG CTAGACTCGG AGCTGCAAAA GGAGACCCTG 
GCCATCTGGC CTCACCTGTC CCAGAAGATG CTAGACCTGC TTGTGCCCAT GCCCAAAGCC 
TCTGACCTGA CTGTGGGCAA AATCTATGCA GCAATGATGA TCATGGACTA CTATAAACAG 
AGTAAGGTGA AGAAACAGAG GCAGCAGCTG GAGGAACAGA AAAATGCACC CATGTTCCAG 
CGAATGGAGC CCTCATCTCT GCCTCAGGAG ATCATTGCTA ATGCCAAAGC CCTGCCCTAT 
CTCCAGCAGG ACCCCGTTTC AGGCCTGAGT GGCCGTAGTG GATACCCTTC GATGAGTCCA 
CTCTCCCCCC AGGAAATATT CCAATTGGCT TGTATGGATC CAGCCGATGA TGGACAGTTC 
CAGGAACATC AGTCTCTGGA GCCAGAGGTT AGTGAATTAA AAAGCGTGCA GTCCTCTAAC 
CATGGCATCT ACCTTCCTTC GGACACCCAG GAGCATACGG GATCTGGGAG GGCATCTTCT 
ATGCCACGCC TGACTGTGGA TCCCCAGGTG GTGACAGATA CTAGCTCCAT GAGACGTTCA 
TTTTCCACTA TTCGGGATAA GCGTTCAAAT TCCTCCTGGT TGGAGGAATT CTCCATGGAG 
AGAAGCAGTG AAAACACCTA CAAGTCACGT CGTCGGAGTT ACCACTCCTC CCTGAGACTG 
TCAGCTCATC GCCTGAATTC TGACTCAGGC CACAAGTCTG ACACCCACCG CTCAGGGGGT 
AGAGAGCGGG GACGATCGAA AGAGCGAAAG CATCTTCTCT CTCCTGATGT CTCCCGCTGC 
AACTCAGAGG AGCGAGGGAC CCAGGCCGAC TGGGAGTCCC CAGAGCGCCA ACAGTCCAGG 
TCTCCCAGTG AGGCCAGGTC ACAGACCCCC AACAGACAGG GCACGGGCTC CCTGAGCGAG 
AGCTCCATCC CCTCCATCTC TGACACCAGC ACCCCAAGAC GAAGTCGCCG GCAGCTCCCA 
CCCGTCCCAC CAAAGCCTCG GCCCCTCCTC TCCTACAGCT CCCTGATTCG ACACTCCGGG 
AGCATCTCCC CGCCGGCCGA CGGAAGCGAG AGGGGCTCCC CGCTGACCCC CCAGGCGCTG 
GAGAGCACGG AGGCCGGCCT GGCGGAGCCC TCGGGCCCCC GGCACGGGCA CGGGCAGGGG 
CACGGGCAGG GGCACGGGCA GCAGAGCCAG CACTGCTCCC CGCAGCGCTA CATCTCCGAG 
CCCTACCTGG CCCTGCACGA AGACTCCCAC GCGTCGGACT GTGGCGAGGA GGAGACGCTC 
ACCTTCGAGG CCGCCGTGGC CACCAGCCTG GGCCGCTCCA ACACCATCGG CTCGGCCCCG 
CCGCTGCGGC ACAGCTGGCA GATGCCCAAC GGGCACTATC GGCGGCGGCG GCGCGGGGGG 
CCGGGGCCGG GCGTGATGTG CGGGGCGGTC AGCGACCTCC TGAGTGACAC GGAAGAAGAC 
GACAAGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_022420936.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022276644.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OCe289502
Clone ID Related Accession (Same CDS sequence)	XM_022420936.1
Accession Version	XM_022420936.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGGAC CGAGCCCAGG CCGCAGGCTG CCCGAGCCCG CCCCCTGCCC CGCCGCGGCC 
GGGAGCCCTG AGCCCTGGGC CGCGGGCCGC CGCCGCCACG TCCTGGCCCT GCCTGAGGAG 
GAGGCATGGA GGCCCCAGCA GTGCTCCCTG CTCGAGGCCG ACGCCTCGGA CGAGGTGGGC 
ATGCTGCCGG CCTCCCCGCG CGCCGCCGCC TCCGGCGTCG ACAACTTCTT CCCGGTGCAG 
GAGGGCGAGG GCGCGGGCTG GGAGGGCGGT GCGGCCCTGG ACGCCGGCGC CGGCCCCTTC 
CTCCCGGTGA GCCCCGAGGT GATGAAGCGG CGGCGCGGGG GCCTCATCGA GCAGCGCGAC 
ATCATCAAGG CCCACGAGGC GCACAAGATG CAGAGCACCC CGCAGGCGCG GCGCAAGGAG 
TGGGAGATGG CTCGCTTCGG GGAGGCGGTG GTCGGCAGGC CGGGGTCGGG CGATGGAGAC 
TCGGACCAGA GCAGGAACCG GCAAGGAACC CCGGTGCCGG CCTCGGGGCC GGCGGCCGCC 
TACAAGCAGT CGAAAGCGCA GAGGGCGCGG ACTATGGCTT TGTACAACCC CATTCCCGTC 
CGGCAGAACT GTTTCACCGT CAACAGATCC CTGTTCATCT TCGGAGAAGA TAACATTGTC 
AGGAAATACG CCAAGAAGCT CATCGACTGG CCGCCGTTTG AGTACATGAT TTTGGCAACC 
ATCATTGCCA ACTGCATTGT CTTGGCCCTG GAACAGCATC TTCCTGAGGA TGACAAGACC 
CCGATGTCCC GCAGACTGGA GAAGACAGAG CCGTATTTCA TTGGGATCTT TTGCTTTGAA 
GCCGGTATCA AAATTGTGGC CCTGGGGTTC ATCTTTCATA AGGGCTCGTA CCTCCGCAAT 
GGCTGGAATG TCATGGACTT CATCGTGGTC CTCAGTGGCA TCCTGGCCAC TGCGGGGACT 
CACTTCAACA CACACGTGGA CCTGAGGACC CTCCGGGCTG TGCGTGTCCT GAGACCCCTG 
AAGCTCGTGT CAGGGATTCC TAGCCTGCAG ATCGTGTTGA AGTCTATCAT GAAGGCCATG 
GTGCCTCTTC TGCAAATTGG TCTCCTGCTC TTCTTTGCCA TCTTGATGTT TGCTATCATT 
GGCTTAGAGT TCTACAGTGG GAAGTTGCAC CGAGCATGTT TCACGAACAA TTCAGGTATT 
CTGGAAGGAT TTGACCCCCC TCACCCATGT GGTGTGCAGG GCTGCCCAGC TGGCTACGAA 
TGCCGGGACT GGATTGGCCC CAATGATGGG ATCACCCAGT TTGATAACAT CCTTTTTGCT 
GTGCTGACTG TCTTCCAATG CATCACCATG GAAGGATGGA CCACCGTGCT GTACAATACC 
AATGATGCCT TAGGAGCCAC CTGGAACTGG CTGTACTTCA TCCCCCTCAT CATCATTGGA 
TCCTTCTTTG TTCTCAACCT AGTTCTGGGA GTGCTTTCCG GGGAGTTTGC CAAAGAGAGA 
GAGAGAGTGG AGAACCGGAG AGCTTTCATG AAACTGCGGC GGCAGCAGCA GATTGAGCGG 
GAGCTGAACG GGTACCGCGC CTGGATAGAC AAAGCAGAGG AAGTCATGCT TGCTGAAGAA 
AATAAAAATG CTGGGACGTC AGCCTTAGAA GTGCTTCGAA GGGCAACCAT CAAAAGGAGC 
CGGACAGAGG CCATGACTCG AGACTCCAGT GATGAACACT GTGTCGATAT CTCCTCTGTG 
GGCACACCTC TCGCCCGAGC CAGCATCAAA AGTGCAAAGG TGGATGGGGC CTCCTATTTC 
CGGCACAAAG AAAGGCTTCT GCGCATCTCT ATCCGCCACA TGGTGAAATC CCAGGTGTTT 
TACTGGATTG TGTTGAGCCT TGTGGCTCTC AACACTGCCT GTGTGGCCAT CGTCCATCAT 
AACCAGCCCC AGTGGCTCAC CCATCTCCTC TACTATGCAG AATTTTTGTT TCTGGGACTG 
TTCCTCTTGG AGATGTCCCT GAAGATGTAT GGCATGGGAC CTCGACTTTA TTTTCACTCT 
TCATTCAACT GTTTTGATTT TGGGGTCACA GTGGGCAGCA TCTTTGAAGT GGTCTGGGCA 
ATCTTCAGAC CTGGCACATC TTTTGGAATC AGTGTCTTAC GGGCTCTCCG GCTTCTAAGA 
ATATTTAAAA TAACCAAGTA TTGGGCATCT CTGCGAAATT TGGTGGTCTC CTTGATGAGC 
TCTATGAAGT CTATCATCAG TTTGCTCTTC CTCCTCTTCC TCTTCATCGT TGTCTTTGCT 
CTCCTAGGAA TGCAGCTGTT TGGAGGCAGG TTTAACTTTA ATGATGGGAC TCCTTCAGCA 
AACTTTGATA CCTTCCCTGC AGCCATCATG ACTGTATTCC AGATCCTAAC AGGTGAGGAC 
TGGAATGAGG TGATGTACAA TGGGATCCGT TCCCAGGGTG GAGTTAGCTC AGGCATGTGG 
TCAGCTATCT ATTTCATTGT GCTCACCTTG TTTGGAAACT ACACACTGCT GAATGTGTTC 
TTGGCCATTG CTGTGGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA TGAACAAGAA 
GAAGAGGAGG CTTTTAACCA GAAACATGCA TTGCAGAAGG CCAAGGAGGT CAGCCCGATG 
TCTGCACCCA ACATGCCTTC TATCGAAAGA GACAGAAGGA GAAGACACCA CATGTCGATG 
TGGGAGCCAC GCAGCAGCCA CCTGAGGGAG CGGAGGCGCC GGCACCACAT GTCGGTGTGG 
GAGCAGCGCA CCAGCCAGCT GAGAAGGCAC ATGCAGATGT CCAGCCAGGA GGCCCTCAAT 
AAAGAGGAGG CCCCACCGAT GAACCCCCTC AACCCCCTCA ACCCGCTGAG CCCCCTCAAC 
CCGCTCAACG CCCACCCCAG CCTCTACCGG AGACCTAGGC CTATTGAGGG CCTGGGCCTG 
GGCCTGGAGA AGTGTGAGGA GGAGCGCATC ATCAGCCGCG GGGGGTCCCT CAAGGGGGAC 
GGAGGGGACC TGTCGAGTGC CCTGGACAAC CAGAGGAGCC CTTTGTCCCT GGGCAAACGG 
GAGCCACCAT GGCTGGCCAG GCCCTGTCAT GGGAACTGTG ATCCGACCCA GCAGGAGGCT 
GGGGGAGGGG AGACCGTGGT GACCTTCGAG GACCGGGCCA GGCACAGGCA GAGCCAGCGG 
CGCAGCCGGC ACCGTCGTGT CAGGACGGAA GGCAAGGAGC TCTCCTCCAC CTCCCGGAGC 
AGGTCTGTCA GCCAGGAGCG GAGTCTGGAT GAGGGGGTGC TCACCGACGG GGAGAAGGAG 
CGAGAGCCCA GGGGCGGCCA TGCAGGCAAA GAACCCACCA TCCAAGAAGA AGACAGAGGC 
CAAGACTTAA GGAGGACGAA CAGTCTGATG GTGCCCAGAG GCTCTGGGCT GGCAGGAGCC 
CTTGATGAGG CCAACACACC CCTAGTCCTG CCCCACCACG ATCTGGAAGT GGGGAAGGAC 
ACGGTGCTGA CGGAGCAGGA GCCGGAGGGC AGCAGCGAGC AGGCCCTGCT GGGGGATGTG 
CAGTTGGACA TTGGCAGGGT CATCAGCCAG AGTGAGCCTG ACCTCTCCTG CGTCACAGCC 
AACACAGACA AGGCCACCAC CGACAGCACC AGCGTCACCG TCGCCATCCC TGATGTGGGC 
CCCTTGATGG ACTCAACTGT GATTCACATT AGCAACAAAA CTGATGGAGA AGCCAGTCCC 
TTGAAGGAGG CTGAGATCAA AGAGGAGGAG GAGGAAATGG AGAAGAAGCA GCAGAAGAAG 
GAGAAGCGTG AGACGGGCAA AGCCATGGTG CCCCACAGCT CTATGTTCAT CTTCAGCACC 
ACCAACCCGA TTCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT TGAGATGTGC 
ATTCTCCTGG TGATCGCCGC CAGCAGCATT GCCCTGGCAG CAGAGGACCC CGTCCTGACC 
AACTCGGAGC GCAACAAAGT CCTGAGGTAT TTTGACTATG TTTTCACTGG CGTGTTCACC 
TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATCC TACAGGATGG GTCCTACTTC 
CGAGACCTGT GGAACATCCT GGACTTTGTG GTGGTGGTTG GTGCATTGGT GGCCTTTGCT 
TTGGCAACAA ACAAGGGGCG AGACATCAAG ACCATCAAAT CCCTGAGGGT GCTCCGAGTT 
CTGAGGCCAC TGAAGACCAT CAAGCGCTTG CCCAAACTCA AGGCTGTCTT TGACTGTGTG 
GTGACCTCCT TGAAGAATGT CTTCAACATA CTCATCGTCT ATAAGCTCTT CATGTTCATC 
TTTGCTGTCA TTGCGGTTCA GCTCTTCAAG GGAAAATTCT TTTATTGCAC AGACAGTTCC 
AAGGACACAG AGAAGGAGTG CATAGGCAAC TACGTAGATC ATGAAAAAAA CAAGATGGAG 
GTGAAAGGCC GGGAATGGAA GCGCCATGAA TTCCACTACG ACAACATTAT CTGGGCCCTG 
CTGACCCTCT TCACCGTCTC CACGGGGGAA GGATGGCCTC AAGTCTTACA GCATTCTGTC 
GATGTGACAG AGGAAGACCG AGGACCAAGC CGCAGCAACC GCATGGAGAT GTCCATCTTC 
TATGTGGTCT ACTTTGTGGT CTTCCCTTTC TTCTTTGTCA ATATCTTTGT GGCTCTGATC 
ATCATCACCT TCCAGGAACA AGGGGACAAG ATGATGGAGG AATGCAGCCT GGAGAAGAAC 
GAGAGGGCGT GCATTGACTT CGCCATCAGT GCCAAACCTC TCACCCGCTA CATGCCACAG 
AACAGGCACA CCTTCCAGTA CCGCGTCTGG CACTTCGTGG TGTCTCCGTC CTTTGAGTAC 
ACCATCATGG CCATGATCGC CCTGAACACC GTCGTGCTGA TGATGAAGTA CTACTCTGCT 
CCCTGTACCT ATGAACTGGC CCTGAAGTAC CTGAATATTG CCTTCACCAT GGTGTTTTCC 
CTGGAATGTG TCCTAAAGAT CATCGCTTTT GGCTTCTTGA ACTACTTTCG AGACACCTGG 
AACATCTTTG ACTTCATCAC TGTGATTGGC AGTATCACAG AAATCATACT GACAGACAGC 
AAGCTGGTAA ACACCAGTGG TTTCAACATG AGCTTTCTGA AGCTCTTCCG TGCCGCCCGC 
CTCATCAAGC TTCTGCGGCA GGGCTACACC ATCCGTATTT TACTCTGGAC CTTCGTGCAG 
TCCTTTAAGG CCCTCCCCTA CGTCTGCCTT TTGATTGCCA TGCTTTTCTT CATCTATGCC 
ATCATTGGGA TGCAGGTATT TGGAAACATA AAATTAGATG AGGAGAGTCA CATCAACCGG 
CACAACAACT TCCGGAGTTT CTTTGGGTCC CTCATGTTAC TCTTCAGGAG TGCCACGGGA 
GAGGCCTGGC AGGAGATAAT GCTGTCGTGC CTCGGGGAGA AAGGCTGCGA GCCCGACACC 
ACTGCGCCAT CGGGGCAGAA CGAGAATGAG CGCTGTGGCA CCGATCTGGC CTATGTTTAC 
TTTGTCTCCT TCATCTTCTT CTGCTCTTTC TTGATGCTCA ACTTGTTTGT GGCCGTCATC 
ATGGACAACT TCGAGTACCT GACTCGGGAC TCCTCCATCT TAGGGCCCCA CCACTTGGAT 
GAGTTTGTCC GCGTCTGGGC AGAGTACGAC CGAGCTGCAT GCGGCAGGAT CCCGTATAAG 
GATATGTACA AATTAGTGCG GGTGATCTCG CCGCCTCTGG GCCTGGGAGA GAACTGCCCC 
TACAGGGTGG CTTGCAAGAG GTTGGTCCTA ATGAATATGC CAGTAGCTGA GGACATGACA 
GTCCACTTCA CCTCCACACT GATGGCTCTG ATCCGGACAG CCCTGGACAT CAAAATCGCC 
AAAGGTGGTG CAGACAGGCA GCAGCTAGAC TCGGAGCTGC AAAAGGAGAC CCTGGCCATC 
TGGCCTCACC TGTCCCAGAA GATGCTAGAC CTGCTTGTGC CCATGCCCAA AGCCTCTGAC 
CTGACTGTGG GCAAAATCTA TGCAGCAATG ATGATCATGG ACTACTATAA ACAGAGTAAG 
GTGAAGAAAC AGAGGCAGCA GCTGGAGGAA CAGAAAAATG CACCCATGTT CCAGCGAATG 
GAGCCCTCAT CTCTGCCTCA GGAGATCATT GCTAATGCCA AAGCCCTGCC CTATCTCCAG 
CAGGACCCCG TTTCAGGCCT GAGTGGCCGT AGTGGATACC CTTCGATGAG TCCACTCTCC 
CCCCAGGAAA TATTCCAATT GGCTTGTATG GATCCAGCCG ATGATGGACA GTTCCAGGAA 
CATCAGTCTC TGGAGCCAGA GGTTAGTGAA TTAAAAAGCG TGCAGTCCTC TAACCATGGC 
ATCTACCTTC CTTCGGACAC CCAGGAGCAT ACGGGATCTG GGAGGGCATC TTCTATGCCA 
CGCCTGACTG TGGATCCCCA GGTGGTGACA GATACTAGCT CCATGAGACG TTCATTTTCC 
ACTATTCGGG ATAAGCGTTC AAATTCCTCC TGGTTGGAGG AATTCTCCAT GGAGAGAAGC 
AGTGAAAACA CCTACAAGTC ACGTCGTCGG AGTTACCACT CCTCCCTGAG ACTGTCAGCT 
CATCGCCTGA ATTCTGACTC AGGCCACAAG TCTGACACCC ACCGCTCAGG GGGTAGAGAG 
CGGGGACGAT CGAAAGAGCG AAAGCATCTT CTCTCTCCTG ATGTCTCCCG CTGCAACTCA 
GAGGAGCGAG GGACCCAGGC CGACTGGGAG TCCCCAGAGC GCCAACAGTC CAGGTCTCCC 
AGTGAGGCCA GGTCACAGAC CCCCAACAGA CAGGGCACGG GCTCCCTGAG CGAGAGCTCC 
ATCCCCTCCA TCTCTGACAC CAGCACCCCA AGACGAAGTC GCCGGCAGCT CCCACCCGTC 
CCACCAAAGC CTCGGCCCCT CCTCTCCTAC AGCTCCCTGA TTCGACACTC CGGGAGCATC 
TCCCCGCCGG CCGACGGAAG CGAGAGGGGC TCCCCGCTGA CCCCCCAGGC GCTGGAGAGC 
ACGGAGGCCG GCCTGGCGGA GCCCTCGGGC CCCCGGCACG GGCACGGGCA GGGGCACGGG 
CAGGGGCACG GGCAGCAGAG CCAGCACTGC TCCCCGCAGC GCTACATCTC CGAGCCCTAC 
CTGGCCCTGC ACGAAGACTC CCACGCGTCG GACTGTGGCG AGGAGGAGAC GCTCACCTTC 
GAGGCCGCCG TGGCCACCAG CCTGGGCCGC TCCAACACCA TCGGCTCGGC CCCGCCGCTG 
CGGCACAGCT GGCAGATGCC CAACGGGCAC TATCGGCGGC GGCGGCGCGG GGGGCCGGGG 
CCGGGCGTGA TGTGCGGGGC GGTCAGCGAC CTCCTGAGTG ACACGGAAGA AGACGACAAG 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276644.1
CDS	6..7391
Translation	MRGPSPGRRLPEPAPCPAAAGSPEPWAAGRRRHVLALPEEEAWRPQQCSLLEADASDEVGMLPASPRAAASGVDNFFPVQEGEGAGWEGGAALDAGAGPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFTNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLGLGLEKCEEERIISRGGSLKGDGGDLSSALDNQRSPLSLGKREPPWLARPCHGNCDPTQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKELSSTSRSRSVSQERSLDEGVLTDGEKEREPRGGHAGKEPTIQEEDRGQDLRRTNSLMVPRGSGLAGALDEANTPLVLPHHDLEVGKDTVLTEQEPEGSSEQALLGDVQLDIGRVISQSEPDLSCVTANTDKATTDSTSVTVAIPDVGPLMDSTVIHISNKTDGEASPLKEAEIKEEEEEMEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALATNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIPYKDMYKLVRVISPPLGLGENCPYRVACKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEHQSLEPEVSELKSVQSSNHGIYLPSDTQEHTGSGRASSMPRLTVDPQVVTDTSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERQQSRSPSEARSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHSGSISPPADGSERGSPLTPQALESTEAGLAEPSGPRHGHGQGHGQGHGQQSQHCSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGVMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_022420936.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022420936.1

ORF Insert Sequence:

ATGCGTGGAC CGAGCCCAGG CCGCAGGCTG CCCGAGCCCG CCCCCTGCCC CGCCGCGGCC 
GGGAGCCCTG AGCCCTGGGC CGCGGGCCGC CGCCGCCACG TCCTGGCCCT GCCTGAGGAG 
GAGGCATGGA GGCCCCAGCA GTGCTCCCTG CTCGAGGCCG ACGCCTCGGA CGAGGTGGGC 
ATGCTGCCGG CCTCCCCGCG CGCCGCCGCC TCCGGCGTCG ACAACTTCTT CCCGGTGCAG 
GAGGGCGAGG GCGCGGGCTG GGAGGGCGGT GCGGCCCTGG ACGCCGGCGC CGGCCCCTTC 
CTCCCGGTGA GCCCCGAGGT GATGAAGCGG CGGCGCGGGG GCCTCATCGA GCAGCGCGAC 
ATCATCAAGG CCCACGAGGC GCACAAGATG CAGAGCACCC CGCAGGCGCG GCGCAAGGAG 
TGGGAGATGG CTCGCTTCGG GGAGGCGGTG GTCGGCAGGC CGGGGTCGGG CGATGGAGAC 
TCGGACCAGA GCAGGAACCG GCAAGGAACC CCGGTGCCGG CCTCGGGGCC GGCGGCCGCC 
TACAAGCAGT CGAAAGCGCA GAGGGCGCGG ACTATGGCTT TGTACAACCC CATTCCCGTC 
CGGCAGAACT GTTTCACCGT CAACAGATCC CTGTTCATCT TCGGAGAAGA TAACATTGTC 
AGGAAATACG CCAAGAAGCT CATCGACTGG CCGCCGTTTG AGTACATGAT TTTGGCAACC 
ATCATTGCCA ACTGCATTGT CTTGGCCCTG GAACAGCATC TTCCTGAGGA TGACAAGACC 
CCGATGTCCC GCAGACTGGA GAAGACAGAG CCGTATTTCA TTGGGATCTT TTGCTTTGAA 
GCCGGTATCA AAATTGTGGC CCTGGGGTTC ATCTTTCATA AGGGCTCGTA CCTCCGCAAT 
GGCTGGAATG TCATGGACTT CATCGTGGTC CTCAGTGGCA TCCTGGCCAC TGCGGGGACT 
CACTTCAACA CACACGTGGA CCTGAGGACC CTCCGGGCTG TGCGTGTCCT GAGACCCCTG 
AAGCTCGTGT CAGGGATTCC TAGCCTGCAG ATCGTGTTGA AGTCTATCAT GAAGGCCATG 
GTGCCTCTTC TGCAAATTGG TCTCCTGCTC TTCTTTGCCA TCTTGATGTT TGCTATCATT 
GGCTTAGAGT TCTACAGTGG GAAGTTGCAC CGAGCATGTT TCACGAACAA TTCAGGTATT 
CTGGAAGGAT TTGACCCCCC TCACCCATGT GGTGTGCAGG GCTGCCCAGC TGGCTACGAA 
TGCCGGGACT GGATTGGCCC CAATGATGGG ATCACCCAGT TTGATAACAT CCTTTTTGCT 
GTGCTGACTG TCTTCCAATG CATCACCATG GAAGGATGGA CCACCGTGCT GTACAATACC 
AATGATGCCT TAGGAGCCAC CTGGAACTGG CTGTACTTCA TCCCCCTCAT CATCATTGGA 
TCCTTCTTTG TTCTCAACCT AGTTCTGGGA GTGCTTTCCG GGGAGTTTGC CAAAGAGAGA 
GAGAGAGTGG AGAACCGGAG AGCTTTCATG AAACTGCGGC GGCAGCAGCA GATTGAGCGG 
GAGCTGAACG GGTACCGCGC CTGGATAGAC AAAGCAGAGG AAGTCATGCT TGCTGAAGAA 
AATAAAAATG CTGGGACGTC AGCCTTAGAA GTGCTTCGAA GGGCAACCAT CAAAAGGAGC 
CGGACAGAGG CCATGACTCG AGACTCCAGT GATGAACACT GTGTCGATAT CTCCTCTGTG 
GGCACACCTC TCGCCCGAGC CAGCATCAAA AGTGCAAAGG TGGATGGGGC CTCCTATTTC 
CGGCACAAAG AAAGGCTTCT GCGCATCTCT ATCCGCCACA TGGTGAAATC CCAGGTGTTT 
TACTGGATTG TGTTGAGCCT TGTGGCTCTC AACACTGCCT GTGTGGCCAT CGTCCATCAT 
AACCAGCCCC AGTGGCTCAC CCATCTCCTC TACTATGCAG AATTTTTGTT TCTGGGACTG 
TTCCTCTTGG AGATGTCCCT GAAGATGTAT GGCATGGGAC CTCGACTTTA TTTTCACTCT 
TCATTCAACT GTTTTGATTT TGGGGTCACA GTGGGCAGCA TCTTTGAAGT GGTCTGGGCA 
ATCTTCAGAC CTGGCACATC TTTTGGAATC AGTGTCTTAC GGGCTCTCCG GCTTCTAAGA 
ATATTTAAAA TAACCAAGTA TTGGGCATCT CTGCGAAATT TGGTGGTCTC CTTGATGAGC 
TCTATGAAGT CTATCATCAG TTTGCTCTTC CTCCTCTTCC TCTTCATCGT TGTCTTTGCT 
CTCCTAGGAA TGCAGCTGTT TGGAGGCAGG TTTAACTTTA ATGATGGGAC TCCTTCAGCA 
AACTTTGATA CCTTCCCTGC AGCCATCATG ACTGTATTCC AGATCCTAAC AGGTGAGGAC 
TGGAATGAGG TGATGTACAA TGGGATCCGT TCCCAGGGTG GAGTTAGCTC AGGCATGTGG 
TCAGCTATCT ATTTCATTGT GCTCACCTTG TTTGGAAACT ACACACTGCT GAATGTGTTC 
TTGGCCATTG CTGTGGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA TGAACAAGAA 
GAAGAGGAGG CTTTTAACCA GAAACATGCA TTGCAGAAGG CCAAGGAGGT CAGCCCGATG 
TCTGCACCCA ACATGCCTTC TATCGAAAGA GACAGAAGGA GAAGACACCA CATGTCGATG 
TGGGAGCCAC GCAGCAGCCA CCTGAGGGAG CGGAGGCGCC GGCACCACAT GTCGGTGTGG 
GAGCAGCGCA CCAGCCAGCT GAGAAGGCAC ATGCAGATGT CCAGCCAGGA GGCCCTCAAT 
AAAGAGGAGG CCCCACCGAT GAACCCCCTC AACCCCCTCA ACCCGCTGAG CCCCCTCAAC 
CCGCTCAACG CCCACCCCAG CCTCTACCGG AGACCTAGGC CTATTGAGGG CCTGGGCCTG 
GGCCTGGAGA AGTGTGAGGA GGAGCGCATC ATCAGCCGCG GGGGGTCCCT CAAGGGGGAC 
GGAGGGGACC TGTCGAGTGC CCTGGACAAC CAGAGGAGCC CTTTGTCCCT GGGCAAACGG 
GAGCCACCAT GGCTGGCCAG GCCCTGTCAT GGGAACTGTG ATCCGACCCA GCAGGAGGCT 
GGGGGAGGGG AGACCGTGGT GACCTTCGAG GACCGGGCCA GGCACAGGCA GAGCCAGCGG 
CGCAGCCGGC ACCGTCGTGT CAGGACGGAA GGCAAGGAGC TCTCCTCCAC CTCCCGGAGC 
AGGTCTGTCA GCCAGGAGCG GAGTCTGGAT GAGGGGGTGC TCACCGACGG GGAGAAGGAG 
CGAGAGCCCA GGGGCGGCCA TGCAGGCAAA GAACCCACCA TCCAAGAAGA AGACAGAGGC 
CAAGACTTAA GGAGGACGAA CAGTCTGATG GTGCCCAGAG GCTCTGGGCT GGCAGGAGCC 
CTTGATGAGG CCAACACACC CCTAGTCCTG CCCCACCACG ATCTGGAAGT GGGGAAGGAC 
ACGGTGCTGA CGGAGCAGGA GCCGGAGGGC AGCAGCGAGC AGGCCCTGCT GGGGGATGTG 
CAGTTGGACA TTGGCAGGGT CATCAGCCAG AGTGAGCCTG ACCTCTCCTG CGTCACAGCC 
AACACAGACA AGGCCACCAC CGACAGCACC AGCGTCACCG TCGCCATCCC TGATGTGGGC 
CCCTTGATGG ACTCAACTGT GATTCACATT AGCAACAAAA CTGATGGAGA AGCCAGTCCC 
TTGAAGGAGG CTGAGATCAA AGAGGAGGAG GAGGAAATGG AGAAGAAGCA GCAGAAGAAG 
GAGAAGCGTG AGACGGGCAA AGCCATGGTG CCCCACAGCT CTATGTTCAT CTTCAGCACC 
ACCAACCCGA TTCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT TGAGATGTGC 
ATTCTCCTGG TGATCGCCGC CAGCAGCATT GCCCTGGCAG CAGAGGACCC CGTCCTGACC 
AACTCGGAGC GCAACAAAGT CCTGAGGTAT TTTGACTATG TTTTCACTGG CGTGTTCACC 
TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATCC TACAGGATGG GTCCTACTTC 
CGAGACCTGT GGAACATCCT GGACTTTGTG GTGGTGGTTG GTGCATTGGT GGCCTTTGCT 
TTGGCAACAA ACAAGGGGCG AGACATCAAG ACCATCAAAT CCCTGAGGGT GCTCCGAGTT 
CTGAGGCCAC TGAAGACCAT CAAGCGCTTG CCCAAACTCA AGGCTGTCTT TGACTGTGTG 
GTGACCTCCT TGAAGAATGT CTTCAACATA CTCATCGTCT ATAAGCTCTT CATGTTCATC 
TTTGCTGTCA TTGCGGTTCA GCTCTTCAAG GGAAAATTCT TTTATTGCAC AGACAGTTCC 
AAGGACACAG AGAAGGAGTG CATAGGCAAC TACGTAGATC ATGAAAAAAA CAAGATGGAG 
GTGAAAGGCC GGGAATGGAA GCGCCATGAA TTCCACTACG ACAACATTAT CTGGGCCCTG 
CTGACCCTCT TCACCGTCTC CACGGGGGAA GGATGGCCTC AAGTCTTACA GCATTCTGTC 
GATGTGACAG AGGAAGACCG AGGACCAAGC CGCAGCAACC GCATGGAGAT GTCCATCTTC 
TATGTGGTCT ACTTTGTGGT CTTCCCTTTC TTCTTTGTCA ATATCTTTGT GGCTCTGATC 
ATCATCACCT TCCAGGAACA AGGGGACAAG ATGATGGAGG AATGCAGCCT GGAGAAGAAC 
GAGAGGGCGT GCATTGACTT CGCCATCAGT GCCAAACCTC TCACCCGCTA CATGCCACAG 
AACAGGCACA CCTTCCAGTA CCGCGTCTGG CACTTCGTGG TGTCTCCGTC CTTTGAGTAC 
ACCATCATGG CCATGATCGC CCTGAACACC GTCGTGCTGA TGATGAAGTA CTACTCTGCT 
CCCTGTACCT ATGAACTGGC CCTGAAGTAC CTGAATATTG CCTTCACCAT GGTGTTTTCC 
CTGGAATGTG TCCTAAAGAT CATCGCTTTT GGCTTCTTGA ACTACTTTCG AGACACCTGG 
AACATCTTTG ACTTCATCAC TGTGATTGGC AGTATCACAG AAATCATACT GACAGACAGC 
AAGCTGGTAA ACACCAGTGG TTTCAACATG AGCTTTCTGA AGCTCTTCCG TGCCGCCCGC 
CTCATCAAGC TTCTGCGGCA GGGCTACACC ATCCGTATTT TACTCTGGAC CTTCGTGCAG 
TCCTTTAAGG CCCTCCCCTA CGTCTGCCTT TTGATTGCCA TGCTTTTCTT CATCTATGCC 
ATCATTGGGA TGCAGGTATT TGGAAACATA AAATTAGATG AGGAGAGTCA CATCAACCGG 
CACAACAACT TCCGGAGTTT CTTTGGGTCC CTCATGTTAC TCTTCAGGAG TGCCACGGGA 
GAGGCCTGGC AGGAGATAAT GCTGTCGTGC CTCGGGGAGA AAGGCTGCGA GCCCGACACC 
ACTGCGCCAT CGGGGCAGAA CGAGAATGAG CGCTGTGGCA CCGATCTGGC CTATGTTTAC 
TTTGTCTCCT TCATCTTCTT CTGCTCTTTC TTGATGCTCA ACTTGTTTGT GGCCGTCATC 
ATGGACAACT TCGAGTACCT GACTCGGGAC TCCTCCATCT TAGGGCCCCA CCACTTGGAT 
GAGTTTGTCC GCGTCTGGGC AGAGTACGAC CGAGCTGCAT GCGGCAGGAT CCCGTATAAG 
GATATGTACA AATTAGTGCG GGTGATCTCG CCGCCTCTGG GCCTGGGAGA GAACTGCCCC 
TACAGGGTGG CTTGCAAGAG GTTGGTCCTA ATGAATATGC CAGTAGCTGA GGACATGACA 
GTCCACTTCA CCTCCACACT GATGGCTCTG ATCCGGACAG CCCTGGACAT CAAAATCGCC 
AAAGGTGGTG CAGACAGGCA GCAGCTAGAC TCGGAGCTGC AAAAGGAGAC CCTGGCCATC 
TGGCCTCACC TGTCCCAGAA GATGCTAGAC CTGCTTGTGC CCATGCCCAA AGCCTCTGAC 
CTGACTGTGG GCAAAATCTA TGCAGCAATG ATGATCATGG ACTACTATAA ACAGAGTAAG 
GTGAAGAAAC AGAGGCAGCA GCTGGAGGAA CAGAAAAATG CACCCATGTT CCAGCGAATG 
GAGCCCTCAT CTCTGCCTCA GGAGATCATT GCTAATGCCA AAGCCCTGCC CTATCTCCAG 
CAGGACCCCG TTTCAGGCCT GAGTGGCCGT AGTGGATACC CTTCGATGAG TCCACTCTCC 
CCCCAGGAAA TATTCCAATT GGCTTGTATG GATCCAGCCG ATGATGGACA GTTCCAGGAA 
CATCAGTCTC TGGAGCCAGA GGTTAGTGAA TTAAAAAGCG TGCAGTCCTC TAACCATGGC 
ATCTACCTTC CTTCGGACAC CCAGGAGCAT ACGGGATCTG GGAGGGCATC TTCTATGCCA 
CGCCTGACTG TGGATCCCCA GGTGGTGACA GATACTAGCT CCATGAGACG TTCATTTTCC 
ACTATTCGGG ATAAGCGTTC AAATTCCTCC TGGTTGGAGG AATTCTCCAT GGAGAGAAGC 
AGTGAAAACA CCTACAAGTC ACGTCGTCGG AGTTACCACT CCTCCCTGAG ACTGTCAGCT 
CATCGCCTGA ATTCTGACTC AGGCCACAAG TCTGACACCC ACCGCTCAGG GGGTAGAGAG 
CGGGGACGAT CGAAAGAGCG AAAGCATCTT CTCTCTCCTG ATGTCTCCCG CTGCAACTCA 
GAGGAGCGAG GGACCCAGGC CGACTGGGAG TCCCCAGAGC GCCAACAGTC CAGGTCTCCC 
AGTGAGGCCA GGTCACAGAC CCCCAACAGA CAGGGCACGG GCTCCCTGAG CGAGAGCTCC 
ATCCCCTCCA TCTCTGACAC CAGCACCCCA AGACGAAGTC GCCGGCAGCT CCCACCCGTC 
CCACCAAAGC CTCGGCCCCT CCTCTCCTAC AGCTCCCTGA TTCGACACTC CGGGAGCATC 
TCCCCGCCGG CCGACGGAAG CGAGAGGGGC TCCCCGCTGA CCCCCCAGGC GCTGGAGAGC 
ACGGAGGCCG GCCTGGCGGA GCCCTCGGGC CCCCGGCACG GGCACGGGCA GGGGCACGGG 
CAGGGGCACG GGCAGCAGAG CCAGCACTGC TCCCCGCAGC GCTACATCTC CGAGCCCTAC 
CTGGCCCTGC ACGAAGACTC CCACGCGTCG GACTGTGGCG AGGAGGAGAC GCTCACCTTC 
GAGGCCGCCG TGGCCACCAG CCTGGGCCGC TCCAACACCA TCGGCTCGGC CCCGCCGCTG 
CGGCACAGCT GGCAGATGCC CAACGGGCAC TATCGGCGGC GGCGGCGCGG GGGGCCGGGG 
CCGGGCGTGA TGTGCGGGGC GGTCAGCGAC CTCCTGAGTG ACACGGAAGA AGACGACAAG 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_014115114.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970589.1 Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe37410
Clone ID Related Accession (Same CDS sequence)	XM_014115114.1
Accession Version	XM_014115114.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGC AATGTTCTGG GATGGTGCCC GAGATGCGTG GACCGAGCCC AGGCCGCAGG 
CTGCCCGAGC CCGCCCCCTG CCCCGCCGCG GCCGGGAGCC CTGAGCCCTG GGCCGCGGGC 
CGCCGCCGCC ACGTCCTGGC CCTGCCTGAG GAGGAGGCAT GGAGGCCCCA GCAGTGCTCC 
CTGCTCGAGG CCGACGCCTC GGACGAGGTG GGCATGCTGC CGGCCTCCCC GCGCGCCGCC 
GCCTCCGGCG TCGACAACTT CTTCCCGGTG CAGGAGGGCG AGGGCGCGGG CTGGGAGGGC 
GGTGCGGCCC TGGACGCCGG CGCCGGCCCC TTCCTCCCGG TGAGCCCCGA GGTGATGAAG 
CGGCGGCGCG GGGGCCTCAT CGAGCAGCGC GACATCATCA AGGCCCACGA GGCGCACAAG 
ATGCAGAGCA CCCCGCAGGC GCGGCGCAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG 
GTGGTCGGCA GGCCGGGGTC GGGCGATGGA GACTCGGACC AGAGCAGGAA CCGGCAAGGA 
ACCCCGGTGC CGGCCTCGGG GCCGGCGGCC GCCTACAAGC AGTCGAAAGC GCAGAGGGCG 
CGGACTATGG CTTTGTACAA CCCCATTCCC GTCCGGCAGA ACTGTTTCAC CGTCAACAGA 
TCCCTGTTCA TCTTCGGAGA AGATAACATT GTCAGGAAAT ACGCCAAGAA GCTCATCGAC 
TGGCCGCCGT TTGAGTACAT GATTTTGGCA ACCATCATTG CCAACTGCAT TGTCTTGGCC 
CTGGAACAGC ATCTTCCTGA GGATGACAAG ACCCCGATGT CCCGCAGACT GGAGAAGACA 
GAGCCGTATT TCATTGGGAT CTTTTGCTTT GAAGCCGGTA TCAAAATTGT GGCCCTGGGG 
TTCATCTTTC ATAAGGGCTC GTACCTCCGC AATGGCTGGA ATGTCATGGA CTTCATCGTG 
GTCCTCAGTG GCATCCTGGC CACTGCGGGG ACTCACTTCA ACACACACGT GGACCTGAGG 
ACCCTCCGGG CTGTGCGTGT CCTGAGACCC CTGAAGCTCG TGTCAGGGAT TCCTAGCCTG 
CAGATCGTGT TGAAGTCTAT CATGAAGGCC ATGGTGCCTC TTCTGCAAAT TGGTCTCCTG 
CTCTTCTTTG CCATCTTGAT GTTTGCTATC ATTGGCTTAG AGTTCTACAG TGGGAAGTTG 
CACCGAGCAT GTTTCACGAA CAATTCAGGT ATTCTGGAAG GATTTGACCC CCCTCACCCA 
TGTGGTGTGC AGGGCTGCCC AGCTGGCTAC GAATGCCGGG ACTGGATTGG CCCCAATGAT 
GGGATCACCC AGTTTGATAA CATCCTTTTT GCTGTGCTGA CTGTCTTCCA ATGCATCACC 
ATGGAAGGAT GGACCACCGT GCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC 
TGGCTGTACT TCATCCCCCT CATCATCATT GGATCCTTCT TTGTTCTCAA CCTAGTTCTG 
GGAGTGCTTT CCGGGGAGTT TGCCAAAGAG AGAGAGAGAG TGGAGAACCG GAGAGCTTTC 
ATGAAACTGC GGCGGCAGCA GCAGATTGAG CGGGAGCTGA ACGGGTACCG CGCCTGGATA 
GACAAAGCAG AGGAAGTCAT GCTTGCTGAA GAAAATAAAA ATGCTGGGAC GTCAGCCTTA 
GAAGTGCTTC GAAGGGCAAC CATCAAAAGG AGCCGGACAG AGGCCATGAC TCGAGACTCC 
AGTGATGAAC ACTGTGTCGA TATCTCCTCT GTGGGCACAC CTCTCGCCCG AGCCAGCATC 
AAAAGTGCAA AGGTGGATGG GGCCTCCTAT TTCCGGCACA AAGAAAGGCT TCTGCGCATC 
TCTATCCGCC ACATGGTGAA ATCCCAGGTG TTTTACTGGA TTGTGTTGAG CCTTGTGGCT 
CTCAACACTG CCTGTGTGGC CATCGTCCAT CATAACCAGC CCCAGTGGCT CACCCATCTC 
CTCTACTATG CAGAATTTTT GTTTCTGGGA CTGTTCCTCT TGGAGATGTC CCTGAAGATG 
TATGGCATGG GACCTCGACT TTATTTTCAC TCTTCATTCA ACTGTTTTGA TTTTGGGGTC 
ACAGTGGGCA GCATCTTTGA AGTGGTCTGG GCAATCTTCA GACCTGGCAC ATCTTTTGGA 
ATCAGTGTCT TACGGGCTCT CCGGCTTCTA AGAATATTTA AAATAACCAA GTATTGGGCA 
TCTCTGCGAA ATTTGGTGGT CTCCTTGATG AGCTCTATGA AGTCTATCAT CAGTTTGCTC 
TTCCTCCTCT TCCTCTTCAT CGTTGTCTTT GCTCTCCTAG GAATGCAGCT GTTTGGAGGC 
AGGTTTAACT TTAATGATGG GACTCCTTCA GCAAACTTTG ATACCTTCCC TGCAGCCATC 
ATGACTGTAT TCCAGATCCT AACAGGTGAG GACTGGAATG AGGTGATGTA CAATGGGATC 
CGTTCCCAGG GTGGAGTTAG CTCAGGCATG TGGTCAGCTA TCTATTTCAT TGTGCTCACC 
TTGTTTGGAA ACTACACACT GCTGAATGTG TTCTTGGCCA TTGCTGTGGA CAATCTGGCC 
AATGCCCAAG AGCTGACCAA GGATGAACAA GAAGAAGAGG AGGCTTTTAA CCAGAAACAT 
GCATTGCAGA AGGCCAAGGA GGTCAGCCCG ATGTCTGCAC CCAACATGCC TTCTATCGAA 
AGAGACAGAA GGAGAAGACA CCACATGTCG ATGTGGGAGC CACGCAGCAG CCACCTGAGG 
GAGCGGAGGC GCCGGCACCA CATGTCGGTG TGGGAGCAGC GCACCAGCCA GCTGAGAAGG 
CACATGCAGA TGTCCAGCCA GGAGGCCCTC AATAAAGAGG AGGCCCCACC GATGAACCCC 
CTCAACCCCC TCAACCCGCT GAGCCCCCTC AACCCGCTCA ACGCCCACCC CAGCCTCTAC 
CGGAGACCTA GGCCTATTGA GGGCCTGGGC CTGGGCCTGG AGAAGTGTGA GGAGGAGCGC 
ATCATCAGCC GCGGGGGGTC CCTCAAGGGG GACGGAGGGG ACCTGTCGAG TGCCCTGGAC 
AACCAGAGGA GCCCTTTGTC CCTGGGCAAA CGGGAGCCAC CATGGCTGGC CAGGCCCTGT 
CATGGGAACT GTGATCCGAC CCAGCAGGAG GCTGGGGGAG GGGAGACCGT GGTGACCTTC 
GAGGACCGGG CCAGGCACAG GCAGAGCCAG CGGCGCAGCC GGCACCGTCG TGTCAGGACG 
GAAGGCAAGG AGCTCTCCTC CACCTCCCGG AGCAGGTCTG TCAGCCAGGA GCGGAGTCTG 
GATGAGGGGG TGCTCACCGA CGGGGAGAAG GAGCGAGAGC CCAGGGGCGG CCATGCAGGC 
AAAGAACCCA CCATCCAAGA AGAAGACAGA GGCCAAGACT TAAGGAGGAC GAACAGTCTG 
ATGGTGCCCA GAGGCTCTGG GCTGGCAGGA GCCCTTGATG AGGCCAACAC ACCCCTAGTC 
CTGCCCCACC ACGATCTGGA AGTGGGGAAG GACACGGTGC TGACGGAGCA GGAGCCGGAG 
GGCAGCAGCG AGCAGGCCCT GCTGGGGGAT GTGCAGTTGG ACATTGGCAG GGTCATCAGC 
CAGAGTGAGC CTGACCTCTC CTGCGTCACA GCCAACACAG ACAAGGCCAC CACCGACAGC 
ACCAGCGTCA CCGTCGCCAT CCCTGATGTG GGCCCCTTGA TGGACTCAAC TGTGATTCAC 
ATTAGCAACA AAACTGATGG AGAAGCCAGT CCCTTGAAGG AGGCTGAGAT CAAAGAGGAG 
GAGGAGGAAA TGGAGAAGAA GCAGCAGAAG AAGGAGAAGC GTGAGACGGG CAAAGCCATG 
GTGCCCCACA GCTCTATGTT CATCTTCAGC ACCACCAACC CGATTCGCAG GGCCTGCCAC 
TACATCGTGA ACCTGCGCTA CTTTGAGATG TGCATTCTCC TGGTGATCGC CGCCAGCAGC 
ATTGCCCTGG CAGCAGAGGA CCCCGTCCTG ACCAACTCGG AGCGCAACAA AGTGGGTTCA 
CGGGAGCCGT TTGCCAACCT CCGTAATGCC CTGGTCCTGA GGTATTTTGA CTATGTTTTC 
ACTGGCGTGT TCACCTTTGA GATGGTTATA AAGATGATAG ACCAGGGCTT GATCCTACAG 
GATGGGTCCT ACTTCCGAGA CCTGTGGAAC ATCCTGGACT TTGTGGTGGT GGTTGGTGCA 
TTGGTGGCCT TTGCTTTGGC GAACGCTTTG GGAACAAACA AGGGGCGAGA CATCAAGACC 
ATCAAATCCC TGAGGGTGCT CCGAGTTCTG AGGCCACTGA AGACCATCAA GCGCTTGCCC 
AAACTCAAGG CTGTCTTTGA CTGTGTGGTG ACCTCCTTGA AGAATGTCTT CAACATACTC 
ATCGTCTATA AGCTCTTCAT GTTCATCTTT GCTGTCATTG CGGTTCAGCT CTTCAAGGGA 
AAATTCTTTT ATTGCACAGA CAGTTCCAAG GACACAGAGA AGGAGTGCAT AGGCAACTAC 
GTAGATCATG AAAAAAACAA GATGGAGGTG AAAGGCCGGG AATGGAAGCG CCATGAATTC 
CACTACGACA ACATTATCTG GGCCCTGCTG ACCCTCTTCA CCGTCTCCAC GGGGGAAGGA 
TGGCCTCAAG TCTTACAGCA TTCTGTCGAT GTGACAGAGG AAGACCGAGG ACCAAGCCGC 
AGCAACCGCA TGGAGATGTC CATCTTCTAT GTGGTCTACT TTGTGGTCTT CCCTTTCTTC 
TTTGTCAATA TCTTTGTGGC TCTGATCATC ATCACCTTCC AGGAACAAGG GGACAAGATG 
ATGGAGGAAT GCAGCCTGGA GAAGAACGAG AGGGCGTGCA TTGACTTCGC CATCAGTGCC 
AAACCTCTCA CCCGCTACAT GCCACAGAAC AGGCACACCT TCCAGTACCG CGTCTGGCAC 
TTCGTGGTGT CTCCGTCCTT TGAGTACACC ATCATGGCCA TGATCGCCCT GAACACCGTC 
GTGCTGATGA TGAAGTACTA CTCTGCTCCC TGTACCTATG AACTGGCCCT GAAGTACCTG 
AATATTGCCT TCACCATGGT GTTTTCCCTG GAATGTGTCC TAAAGATCAT CGCTTTTGGC 
TTCTTGAACT ACTTTCGAGA CACCTGGAAC ATCTTTGACT TCATCACTGT GATTGGCAGT 
ATCACAGAAA TCATACTGAC AGACAGCAAG CTGGTAAACA CCAGTGGTTT CAACATGAGC 
TTTCTGAAGC TCTTCCGTGC CGCCCGCCTC ATCAAGCTTC TGCGGCAGGG CTACACCATC 
CGTATTTTAC TCTGGACCTT CGTGCAGTCC TTTAAGGCCC TCCCCTACGT CTGCCTTTTG 
ATTGCCATGC TTTTCTTCAT CTATGCCATC ATTGGGATGC AGGTATTTGG AAACATAAAA 
TTAGATGAGG AGAGTCACAT CAACCGGCAC AACAACTTCC GGAGTTTCTT TGGGTCCCTC 
ATGTTACTCT TCAGGAGTGC CACGGGAGAG GCCTGGCAGG AGATAATGCT GTCGTGCCTC 
GGGGAGAAAG GCTGCGAGCC CGACACCACT GCGCCATCGG GGCAGAACGA GAATGAGCGC 
TGTGGCACCG ATCTGGCCTA TGTTTACTTT GTCTCCTTCA TCTTCTTCTG CTCTTTCTTG 
ATGCTCAACT TGTTTGTGGC CGTCATCATG GACAACTTCG AGTACCTGAC TCGGGACTCC 
TCCATCTTAG GGCCCCACCA CTTGGATGAG TTTGTCCGCG TCTGGGCAGA GTACGACCGA 
GCTGCATGCG GCAGGATCCC GTATAAGGAT ATGTACAAAT TAGTGCGGGT GATCTCGCCG 
CCTCTGGGCC TGGGAGAGAA CTGCCCCTAC AGGGTGGCTT GCAAGAGGTT GGTCCTAATG 
AATATGCCAG TAGCTGAGGA CATGACAGTC CACTTCACCT CCACACTGAT GGCTCTGATC 
CGGACAGCCC TGGACATCAA AATCGCCAAA GGTGGTGCAG ACAGGCAGCA GCTAGACTCG 
GAGCTGCAAA AGGAGACCCT GGCCATCTGG CCTCACCTGT CCCAGAAGAT GCTAGACCTG 
CTTGTGCCCA TGCCCAAAGC CTCTGACCTG ACTGTGGGCA AAATCTATGC AGCAATGATG 
ATCATGGACT ACTATAAACA GAGTAAGGTG AAGAAACAGA GGCAGCAGCT GGAGGAACAG 
AAAAATGCAC CCATGTTCCA GCGAATGGAG CCCTCATCTC TGCCTCAGGA GATCATTGCT 
AATGCCAAAG CCCTGCCCTA TCTCCAGCAG GACCCCGTTT CAGGCCTGAG TGGCCGTAGT 
GGATACCCTT CGATGAGTCC ACTCTCCCCC CAGGAAATAT TCCAATTGGC TTGTATGGAT 
CCAGCCGATG ATGGACAGTT CCAGGAACAT CAGTCTCTGG AGCCAGAGGT TAGTGAATTA 
AAAAGCGTGC AGTCCTCTAA CCATGGCATC TACCTTCCTT CGGACACCCA GGAGCATACG 
GGATCTGGGA GGGCATCTTC TATGCCACGC CTGACTGTGG ATCCCCAGGT GGTGACAGAT 
ACTAGCTCCA TGAGACGTTC ATTTTCCACT ATTCGGGATA AGCGTTCAAA TTCCTCCTGG 
TTGGAGGAAT TCTCCATGGA GAGAAGCAGT GAAAACACCT ACAAGTCACG TCGTCGGAGT 
TACCACTCCT CCCTGAGACT GTCAGCTCAT CGCCTGAATT CTGACTCAGG CCACAAGTCT 
GACACCCACC GCTCAGGGGG TAGAGAGCGG GGACGATCGA AAGAGCGAAA GCATCTTCTC 
TCTCCTGATG TCTCCCGCTG CAACTCAGAG GAGCGAGGGA CCCAGGCCGA CTGGGAGTCC 
CCAGAGCGCC AACAGTCCAG GTCTCCCAGT GAGGCCAGGT CACAGACCCC CAACAGACAG 
GGCACGGGCT CCCTGAGCGA GAGCTCCATC CCCTCCATCT CTGACACCAG CACCCCAAGA 
CGAAGTCGCC GGCAGCTCCC ACCCGTCCCA CCAAAGCCTC GGCCCCTCCT CTCCTACAGC 
TCCCTGATTC GACACTCCGG GAGCATCTCC CCGCCGGCCG ACGGAAGCGA GAGGGGCTCC 
CCGCTGACCC CCCAGGCGCT GGAGAGCACG GAGGCCGGCC TGGCGGAGCC CTCGGGCCCC 
CGGCACGGGC ACGGGCAGGG GCACGGGCAG GGGCACGGGC AGCAGAGCCA GCACTGCTCC 
CCGCAGCGCT ACATCTCCGA GCCCTACCTG GCCCTGCACG AAGACTCCCA CGCGTCGGAC 
TGTGGCGAGG AGGAGACGCT CACCTTCGAG GCCGCCGTGG CCACCAGCCT GGGCCGCTCC 
AACACCATCG GCTCGGCCCC GCCGCTGCGG CACAGCTGGC AGATGCCCAA CGGGCACTAT 
CGGCGGCGGC GGCGCGGGGG GCCGGGGCCG GGCGTGATGT GCGGGGCGGT CAGCGACCTC 
CTGAGTGACA CGGAAGAAGA CGACAAGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970589.1
CDS	87..7559
Translation	MEEQCSGMVPEMRGPSPGRRLPEPAPCPAAAGSPEPWAAGRRRHVLALPEEEAWRPQQCSLLEADASDEVGMLPASPRAAASGVDNFFPVQEGEGAGWEGGAALDAGAGPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFTNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLGLGLEKCEEERIISRGGSLKGDGGDLSSALDNQRSPLSLGKREPPWLARPCHGNCDPTQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKELSSTSRSRSVSQERSLDEGVLTDGEKEREPRGGHAGKEPTIQEEDRGQDLRRTNSLMVPRGSGLAGALDEANTPLVLPHHDLEVGKDTVLTEQEPEGSSEQALLGDVQLDIGRVISQSEPDLSCVTANTDKATTDSTSVTVAIPDVGPLMDSTVIHISNKTDGEASPLKEAEIKEEEEEMEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVGSREPFANLRNALVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIPYKDMYKLVRVISPPLGLGENCPYRVACKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEHQSLEPEVSELKSVQSSNHGIYLPSDTQEHTGSGRASSMPRLTVDPQVVTDTSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERQQSRSPSEARSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHSGSISPPADGSERGSPLTPQALESTEAGLAEPSGPRHGHGQGHGQGHGQQSQHCSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGVMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_014115114.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115114.1

ORF Insert Sequence:

ATGGAAGAGC AATGTTCTGG GATGGTGCCC GAGATGCGTG GACCGAGCCC AGGCCGCAGG 
CTGCCCGAGC CCGCCCCCTG CCCCGCCGCG GCCGGGAGCC CTGAGCCCTG GGCCGCGGGC 
CGCCGCCGCC ACGTCCTGGC CCTGCCTGAG GAGGAGGCAT GGAGGCCCCA GCAGTGCTCC 
CTGCTCGAGG CCGACGCCTC GGACGAGGTG GGCATGCTGC CGGCCTCCCC GCGCGCCGCC 
GCCTCCGGCG TCGACAACTT CTTCCCGGTG CAGGAGGGCG AGGGCGCGGG CTGGGAGGGC 
GGTGCGGCCC TGGACGCCGG CGCCGGCCCC TTCCTCCCGG TGAGCCCCGA GGTGATGAAG 
CGGCGGCGCG GGGGCCTCAT CGAGCAGCGC GACATCATCA AGGCCCACGA GGCGCACAAG 
ATGCAGAGCA CCCCGCAGGC GCGGCGCAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG 
GTGGTCGGCA GGCCGGGGTC GGGCGATGGA GACTCGGACC AGAGCAGGAA CCGGCAAGGA 
ACCCCGGTGC CGGCCTCGGG GCCGGCGGCC GCCTACAAGC AGTCGAAAGC GCAGAGGGCG 
CGGACTATGG CTTTGTACAA CCCCATTCCC GTCCGGCAGA ACTGTTTCAC CGTCAACAGA 
TCCCTGTTCA TCTTCGGAGA AGATAACATT GTCAGGAAAT ACGCCAAGAA GCTCATCGAC 
TGGCCGCCGT TTGAGTACAT GATTTTGGCA ACCATCATTG CCAACTGCAT TGTCTTGGCC 
CTGGAACAGC ATCTTCCTGA GGATGACAAG ACCCCGATGT CCCGCAGACT GGAGAAGACA 
GAGCCGTATT TCATTGGGAT CTTTTGCTTT GAAGCCGGTA TCAAAATTGT GGCCCTGGGG 
TTCATCTTTC ATAAGGGCTC GTACCTCCGC AATGGCTGGA ATGTCATGGA CTTCATCGTG 
GTCCTCAGTG GCATCCTGGC CACTGCGGGG ACTCACTTCA ACACACACGT GGACCTGAGG 
ACCCTCCGGG CTGTGCGTGT CCTGAGACCC CTGAAGCTCG TGTCAGGGAT TCCTAGCCTG 
CAGATCGTGT TGAAGTCTAT CATGAAGGCC ATGGTGCCTC TTCTGCAAAT TGGTCTCCTG 
CTCTTCTTTG CCATCTTGAT GTTTGCTATC ATTGGCTTAG AGTTCTACAG TGGGAAGTTG 
CACCGAGCAT GTTTCACGAA CAATTCAGGT ATTCTGGAAG GATTTGACCC CCCTCACCCA 
TGTGGTGTGC AGGGCTGCCC AGCTGGCTAC GAATGCCGGG ACTGGATTGG CCCCAATGAT 
GGGATCACCC AGTTTGATAA CATCCTTTTT GCTGTGCTGA CTGTCTTCCA ATGCATCACC 
ATGGAAGGAT GGACCACCGT GCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC 
TGGCTGTACT TCATCCCCCT CATCATCATT GGATCCTTCT TTGTTCTCAA CCTAGTTCTG 
GGAGTGCTTT CCGGGGAGTT TGCCAAAGAG AGAGAGAGAG TGGAGAACCG GAGAGCTTTC 
ATGAAACTGC GGCGGCAGCA GCAGATTGAG CGGGAGCTGA ACGGGTACCG CGCCTGGATA 
GACAAAGCAG AGGAAGTCAT GCTTGCTGAA GAAAATAAAA ATGCTGGGAC GTCAGCCTTA 
GAAGTGCTTC GAAGGGCAAC CATCAAAAGG AGCCGGACAG AGGCCATGAC TCGAGACTCC 
AGTGATGAAC ACTGTGTCGA TATCTCCTCT GTGGGCACAC CTCTCGCCCG AGCCAGCATC 
AAAAGTGCAA AGGTGGATGG GGCCTCCTAT TTCCGGCACA AAGAAAGGCT TCTGCGCATC 
TCTATCCGCC ACATGGTGAA ATCCCAGGTG TTTTACTGGA TTGTGTTGAG CCTTGTGGCT 
CTCAACACTG CCTGTGTGGC CATCGTCCAT CATAACCAGC CCCAGTGGCT CACCCATCTC 
CTCTACTATG CAGAATTTTT GTTTCTGGGA CTGTTCCTCT TGGAGATGTC CCTGAAGATG 
TATGGCATGG GACCTCGACT TTATTTTCAC TCTTCATTCA ACTGTTTTGA TTTTGGGGTC 
ACAGTGGGCA GCATCTTTGA AGTGGTCTGG GCAATCTTCA GACCTGGCAC ATCTTTTGGA 
ATCAGTGTCT TACGGGCTCT CCGGCTTCTA AGAATATTTA AAATAACCAA GTATTGGGCA 
TCTCTGCGAA ATTTGGTGGT CTCCTTGATG AGCTCTATGA AGTCTATCAT CAGTTTGCTC 
TTCCTCCTCT TCCTCTTCAT CGTTGTCTTT GCTCTCCTAG GAATGCAGCT GTTTGGAGGC 
AGGTTTAACT TTAATGATGG GACTCCTTCA GCAAACTTTG ATACCTTCCC TGCAGCCATC 
ATGACTGTAT TCCAGATCCT AACAGGTGAG GACTGGAATG AGGTGATGTA CAATGGGATC 
CGTTCCCAGG GTGGAGTTAG CTCAGGCATG TGGTCAGCTA TCTATTTCAT TGTGCTCACC 
TTGTTTGGAA ACTACACACT GCTGAATGTG TTCTTGGCCA TTGCTGTGGA CAATCTGGCC 
AATGCCCAAG AGCTGACCAA GGATGAACAA GAAGAAGAGG AGGCTTTTAA CCAGAAACAT 
GCATTGCAGA AGGCCAAGGA GGTCAGCCCG ATGTCTGCAC CCAACATGCC TTCTATCGAA 
AGAGACAGAA GGAGAAGACA CCACATGTCG ATGTGGGAGC CACGCAGCAG CCACCTGAGG 
GAGCGGAGGC GCCGGCACCA CATGTCGGTG TGGGAGCAGC GCACCAGCCA GCTGAGAAGG 
CACATGCAGA TGTCCAGCCA GGAGGCCCTC AATAAAGAGG AGGCCCCACC GATGAACCCC 
CTCAACCCCC TCAACCCGCT GAGCCCCCTC AACCCGCTCA ACGCCCACCC CAGCCTCTAC 
CGGAGACCTA GGCCTATTGA GGGCCTGGGC CTGGGCCTGG AGAAGTGTGA GGAGGAGCGC 
ATCATCAGCC GCGGGGGGTC CCTCAAGGGG GACGGAGGGG ACCTGTCGAG TGCCCTGGAC 
AACCAGAGGA GCCCTTTGTC CCTGGGCAAA CGGGAGCCAC CATGGCTGGC CAGGCCCTGT 
CATGGGAACT GTGATCCGAC CCAGCAGGAG GCTGGGGGAG GGGAGACCGT GGTGACCTTC 
GAGGACCGGG CCAGGCACAG GCAGAGCCAG CGGCGCAGCC GGCACCGTCG TGTCAGGACG 
GAAGGCAAGG AGCTCTCCTC CACCTCCCGG AGCAGGTCTG TCAGCCAGGA GCGGAGTCTG 
GATGAGGGGG TGCTCACCGA CGGGGAGAAG GAGCGAGAGC CCAGGGGCGG CCATGCAGGC 
AAAGAACCCA CCATCCAAGA AGAAGACAGA GGCCAAGACT TAAGGAGGAC GAACAGTCTG 
ATGGTGCCCA GAGGCTCTGG GCTGGCAGGA GCCCTTGATG AGGCCAACAC ACCCCTAGTC 
CTGCCCCACC ACGATCTGGA AGTGGGGAAG GACACGGTGC TGACGGAGCA GGAGCCGGAG 
GGCAGCAGCG AGCAGGCCCT GCTGGGGGAT GTGCAGTTGG ACATTGGCAG GGTCATCAGC 
CAGAGTGAGC CTGACCTCTC CTGCGTCACA GCCAACACAG ACAAGGCCAC CACCGACAGC 
ACCAGCGTCA CCGTCGCCAT CCCTGATGTG GGCCCCTTGA TGGACTCAAC TGTGATTCAC 
ATTAGCAACA AAACTGATGG AGAAGCCAGT CCCTTGAAGG AGGCTGAGAT CAAAGAGGAG 
GAGGAGGAAA TGGAGAAGAA GCAGCAGAAG AAGGAGAAGC GTGAGACGGG CAAAGCCATG 
GTGCCCCACA GCTCTATGTT CATCTTCAGC ACCACCAACC CGATTCGCAG GGCCTGCCAC 
TACATCGTGA ACCTGCGCTA CTTTGAGATG TGCATTCTCC TGGTGATCGC CGCCAGCAGC 
ATTGCCCTGG CAGCAGAGGA CCCCGTCCTG ACCAACTCGG AGCGCAACAA AGTGGGTTCA 
CGGGAGCCGT TTGCCAACCT CCGTAATGCC CTGGTCCTGA GGTATTTTGA CTATGTTTTC 
ACTGGCGTGT TCACCTTTGA GATGGTTATA AAGATGATAG ACCAGGGCTT GATCCTACAG 
GATGGGTCCT ACTTCCGAGA CCTGTGGAAC ATCCTGGACT TTGTGGTGGT GGTTGGTGCA 
TTGGTGGCCT TTGCTTTGGC GAACGCTTTG GGAACAAACA AGGGGCGAGA CATCAAGACC 
ATCAAATCCC TGAGGGTGCT CCGAGTTCTG AGGCCACTGA AGACCATCAA GCGCTTGCCC 
AAACTCAAGG CTGTCTTTGA CTGTGTGGTG ACCTCCTTGA AGAATGTCTT CAACATACTC 
ATCGTCTATA AGCTCTTCAT GTTCATCTTT GCTGTCATTG CGGTTCAGCT CTTCAAGGGA 
AAATTCTTTT ATTGCACAGA CAGTTCCAAG GACACAGAGA AGGAGTGCAT AGGCAACTAC 
GTAGATCATG AAAAAAACAA GATGGAGGTG AAAGGCCGGG AATGGAAGCG CCATGAATTC 
CACTACGACA ACATTATCTG GGCCCTGCTG ACCCTCTTCA CCGTCTCCAC GGGGGAAGGA 
TGGCCTCAAG TCTTACAGCA TTCTGTCGAT GTGACAGAGG AAGACCGAGG ACCAAGCCGC 
AGCAACCGCA TGGAGATGTC CATCTTCTAT GTGGTCTACT TTGTGGTCTT CCCTTTCTTC 
TTTGTCAATA TCTTTGTGGC TCTGATCATC ATCACCTTCC AGGAACAAGG GGACAAGATG 
ATGGAGGAAT GCAGCCTGGA GAAGAACGAG AGGGCGTGCA TTGACTTCGC CATCAGTGCC 
AAACCTCTCA CCCGCTACAT GCCACAGAAC AGGCACACCT TCCAGTACCG CGTCTGGCAC 
TTCGTGGTGT CTCCGTCCTT TGAGTACACC ATCATGGCCA TGATCGCCCT GAACACCGTC 
GTGCTGATGA TGAAGTACTA CTCTGCTCCC TGTACCTATG AACTGGCCCT GAAGTACCTG 
AATATTGCCT TCACCATGGT GTTTTCCCTG GAATGTGTCC TAAAGATCAT CGCTTTTGGC 
TTCTTGAACT ACTTTCGAGA CACCTGGAAC ATCTTTGACT TCATCACTGT GATTGGCAGT 
ATCACAGAAA TCATACTGAC AGACAGCAAG CTGGTAAACA CCAGTGGTTT CAACATGAGC 
TTTCTGAAGC TCTTCCGTGC CGCCCGCCTC ATCAAGCTTC TGCGGCAGGG CTACACCATC 
CGTATTTTAC TCTGGACCTT CGTGCAGTCC TTTAAGGCCC TCCCCTACGT CTGCCTTTTG 
ATTGCCATGC TTTTCTTCAT CTATGCCATC ATTGGGATGC AGGTATTTGG AAACATAAAA 
TTAGATGAGG AGAGTCACAT CAACCGGCAC AACAACTTCC GGAGTTTCTT TGGGTCCCTC 
ATGTTACTCT TCAGGAGTGC CACGGGAGAG GCCTGGCAGG AGATAATGCT GTCGTGCCTC 
GGGGAGAAAG GCTGCGAGCC CGACACCACT GCGCCATCGG GGCAGAACGA GAATGAGCGC 
TGTGGCACCG ATCTGGCCTA TGTTTACTTT GTCTCCTTCA TCTTCTTCTG CTCTTTCTTG 
ATGCTCAACT TGTTTGTGGC CGTCATCATG GACAACTTCG AGTACCTGAC TCGGGACTCC 
TCCATCTTAG GGCCCCACCA CTTGGATGAG TTTGTCCGCG TCTGGGCAGA GTACGACCGA 
GCTGCATGCG GCAGGATCCC GTATAAGGAT ATGTACAAAT TAGTGCGGGT GATCTCGCCG 
CCTCTGGGCC TGGGAGAGAA CTGCCCCTAC AGGGTGGCTT GCAAGAGGTT GGTCCTAATG 
AATATGCCAG TAGCTGAGGA CATGACAGTC CACTTCACCT CCACACTGAT GGCTCTGATC 
CGGACAGCCC TGGACATCAA AATCGCCAAA GGTGGTGCAG ACAGGCAGCA GCTAGACTCG 
GAGCTGCAAA AGGAGACCCT GGCCATCTGG CCTCACCTGT CCCAGAAGAT GCTAGACCTG 
CTTGTGCCCA TGCCCAAAGC CTCTGACCTG ACTGTGGGCA AAATCTATGC AGCAATGATG 
ATCATGGACT ACTATAAACA GAGTAAGGTG AAGAAACAGA GGCAGCAGCT GGAGGAACAG 
AAAAATGCAC CCATGTTCCA GCGAATGGAG CCCTCATCTC TGCCTCAGGA GATCATTGCT 
AATGCCAAAG CCCTGCCCTA TCTCCAGCAG GACCCCGTTT CAGGCCTGAG TGGCCGTAGT 
GGATACCCTT CGATGAGTCC ACTCTCCCCC CAGGAAATAT TCCAATTGGC TTGTATGGAT 
CCAGCCGATG ATGGACAGTT CCAGGAACAT CAGTCTCTGG AGCCAGAGGT TAGTGAATTA 
AAAAGCGTGC AGTCCTCTAA CCATGGCATC TACCTTCCTT CGGACACCCA GGAGCATACG 
GGATCTGGGA GGGCATCTTC TATGCCACGC CTGACTGTGG ATCCCCAGGT GGTGACAGAT 
ACTAGCTCCA TGAGACGTTC ATTTTCCACT ATTCGGGATA AGCGTTCAAA TTCCTCCTGG 
TTGGAGGAAT TCTCCATGGA GAGAAGCAGT GAAAACACCT ACAAGTCACG TCGTCGGAGT 
TACCACTCCT CCCTGAGACT GTCAGCTCAT CGCCTGAATT CTGACTCAGG CCACAAGTCT 
GACACCCACC GCTCAGGGGG TAGAGAGCGG GGACGATCGA AAGAGCGAAA GCATCTTCTC 
TCTCCTGATG TCTCCCGCTG CAACTCAGAG GAGCGAGGGA CCCAGGCCGA CTGGGAGTCC 
CCAGAGCGCC AACAGTCCAG GTCTCCCAGT GAGGCCAGGT CACAGACCCC CAACAGACAG 
GGCACGGGCT CCCTGAGCGA GAGCTCCATC CCCTCCATCT CTGACACCAG CACCCCAAGA 
CGAAGTCGCC GGCAGCTCCC ACCCGTCCCA CCAAAGCCTC GGCCCCTCCT CTCCTACAGC 
TCCCTGATTC GACACTCCGG GAGCATCTCC CCGCCGGCCG ACGGAAGCGA GAGGGGCTCC 
CCGCTGACCC CCCAGGCGCT GGAGAGCACG GAGGCCGGCC TGGCGGAGCC CTCGGGCCCC 
CGGCACGGGC ACGGGCAGGG GCACGGGCAG GGGCACGGGC AGCAGAGCCA GCACTGCTCC 
CCGCAGCGCT ACATCTCCGA GCCCTACCTG GCCCTGCACG AAGACTCCCA CGCGTCGGAC 
TGTGGCGAGG AGGAGACGCT CACCTTCGAG GCCGCCGTGG CCACCAGCCT GGGCCGCTCC 
AACACCATCG GCTCGGCCCC GCCGCTGCGG CACAGCTGGC AGATGCCCAA CGGGCACTAT 
CGGCGGCGGC GGCGCGGGGG GCCGGGGCCG GGCGTGATGT GCGGGGCGGT CAGCGACCTC 
CTGAGTGACA CGGAAGAAGA CGACAAGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_014115115.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970590.1 Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe37411
Clone ID Related Accession (Same CDS sequence)	XM_014115115.1
Accession Version	XM_014115115.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGC AATGTTCTGG GATGGTGCCC GAGATGCGTG GACCGAGCCC AGGCCGCAGG 
CTGCCCGAGC CCGCCCCCTG CCCCGCCGCG GCCGGGAGCC CTGAGCCCTG GGCCGCGGGC 
CGCCGCCGCC ACGTCCTGGC CCTGCCTGAG GAGGAGGCAT GGAGGCCCCA GCAGTGCTCC 
CTGCTCGAGG CCGACGCCTC GGACGAGGTG GGCATGCTGC CGGCCTCCCC GCGCGCCGCC 
GCCTCCGGCG TCGACAACTT CTTCCCGGTG CAGGAGGGCG AGGGCGCGGG CTGGGAGGGC 
GGTGCGGCCC TGGACGCCGG CGCCGGCCCC TTCCTCCCGG TGAGCCCCGA GGTGATGAAG 
CGGCGGCGCG GGGGCCTCAT CGAGCAGCGC GACATCATCA AGGCCCACGA GGCGCACAAG 
ATGCAGAGCA CCCCGCAGGC GCGGCGCAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG 
GTGGTCGGCA GGCCGGGGTC GGGCGATGGA GACTCGGACC AGAGCAGGAA CCGGCAAGGA 
ACCCCGGTGC CGGCCTCGGG GCCGGCGGCC GCCTACAAGC AGTCGAAAGC GCAGAGGGCG 
CGGACTATGG CTTTGTACAA CCCCATTCCC GTCCGGCAGA ACTGTTTCAC CGTCAACAGA 
TCCCTGTTCA TCTTCGGAGA AGATAACATT GTCAGGAAAT ACGCCAAGAA GCTCATCGAC 
TGGCCGCCGT TTGAGTACAT GATTTTGGCA ACCATCATTG CCAACTGCAT TGTCTTGGCC 
CTGGAACAGC ATCTTCCTGA GGATGACAAG ACCCCGATGT CCCGCAGACT GGAGAAGACA 
GAGCCGTATT TCATTGGGAT CTTTTGCTTT GAAGCCGGTA TCAAAATTGT GGCCCTGGGG 
TTCATCTTTC ATAAGGGCTC GTACCTCCGC AATGGCTGGA ATGTCATGGA CTTCATCGTG 
GTCCTCAGTG GCATCCTGGC CACTGCGGGG ACTCACTTCA ACACACACGT GGACCTGAGG 
ACCCTCCGGG CTGTGCGTGT CCTGAGACCC CTGAAGCTCG TGTCAGGGAT TCCTAGCCTG 
CAGATCGTGT TGAAGTCTAT CATGAAGGCC ATGGTGCCTC TTCTGCAAAT TGGTCTCCTG 
CTCTTCTTTG CCATCTTGAT GTTTGCTATC ATTGGCTTAG AGTTCTACAG TGGGAAGTTG 
CACCGAGCAT GTTTCACGAA CAATTCAGGT ATTCTGGAAG GATTTGACCC CCCTCACCCA 
TGTGGTGTGC AGGGCTGCCC AGCTGGCTAC GAATGCCGGG ACTGGATTGG CCCCAATGAT 
GGGATCACCC AGTTTGATAA CATCCTTTTT GCTGTGCTGA CTGTCTTCCA ATGCATCACC 
ATGGAAGGAT GGACCACCGT GCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC 
TGGCTGTACT TCATCCCCCT CATCATCATT GGATCCTTCT TTGTTCTCAA CCTAGTTCTG 
GGAGTGCTTT CCGGGGAGTT TGCCAAAGAG AGAGAGAGAG TGGAGAACCG GAGAGCTTTC 
ATGAAACTGC GGCGGCAGCA GCAGATTGAG CGGGAGCTGA ACGGGTACCG CGCCTGGATA 
GACAAAGCAG AGGAAGTCAT GCTTGCTGAA GAAAATAAAA ATGCTGGGAC GTCAGCCTTA 
GAAGTGCTTC GAAGGGCAAC CATCAAAAGG AGCCGGACAG AGGCCATGAC TCGAGACTCC 
AGTGATGAAC ACTGTGTCGA TATCTCCTCT GTGGGCACAC CTCTCGCCCG AGCCAGCATC 
AAAAGTGCAA AGGTGGATGG GGCCTCCTAT TTCCGGCACA AAGAAAGGCT TCTGCGCATC 
TCTATCCGCC ACATGGTGAA ATCCCAGGTG TTTTACTGGA TTGTGTTGAG CCTTGTGGCT 
CTCAACACTG CCTGTGTGGC CATCGTCCAT CATAACCAGC CCCAGTGGCT CACCCATCTC 
CTCTACTATG CAGAATTTTT GTTTCTGGGA CTGTTCCTCT TGGAGATGTC CCTGAAGATG 
TATGGCATGG GACCTCGACT TTATTTTCAC TCTTCATTCA ACTGTTTTGA TTTTGGGGTC 
ACAGTGGGCA GCATCTTTGA AGTGGTCTGG GCAATCTTCA GACCTGGCAC ATCTTTTGGA 
ATCAGTGTCT TACGGGCTCT CCGGCTTCTA AGAATATTTA AAATAACCAA GTATTGGGCA 
TCTCTGCGAA ATTTGGTGGT CTCCTTGATG AGCTCTATGA AGTCTATCAT CAGTTTGCTC 
TTCCTCCTCT TCCTCTTCAT CGTTGTCTTT GCTCTCCTAG GAATGCAGCT GTTTGGAGGC 
AGGTTTAACT TTAATGATGG GACTCCTTCA GCAAACTTTG ATACCTTCCC TGCAGCCATC 
ATGACTGTAT TCCAGATCCT AACAGGTGAG GACTGGAATG AGGTGATGTA CAATGGGATC 
CGTTCCCAGG GTGGAGTTAG CTCAGGCATG TGGTCAGCTA TCTATTTCAT TGTGCTCACC 
TTGTTTGGAA ACTACACACT GCTGAATGTG TTCTTGGCCA TTGCTGTGGA CAATCTGGCC 
AATGCCCAAG AGCTGACCAA GGATGAACAA GAAGAAGAGG AGGCTTTTAA CCAGAAACAT 
GCATTGCAGA AGGCCAAGGA GGTCAGCCCG ATGTCTGCAC CCAACATGCC TTCTATCGAA 
AGAGACAGAA GGAGAAGACA CCACATGTCG ATGTGGGAGC CACGCAGCAG CCACCTGAGG 
GAGCGGAGGC GCCGGCACCA CATGTCGGTG TGGGAGCAGC GCACCAGCCA GCTGAGAAGG 
CACATGCAGA TGTCCAGCCA GGAGGCCCTC AATAAAGAGG AGGCCCCACC GATGAACCCC 
CTCAACCCCC TCAACCCGCT GAGCCCCCTC AACCCGCTCA ACGCCCACCC CAGCCTCTAC 
CGGAGACCTA GGCCTATTGA GGGCCTGGGC CTGGGCCTGG AGAAGTGTGA GGAGGAGCGC 
ATCATCAGCC GCGGGGGGTC CCTCAAGGGG GACGGAGGGG ACCTGTCGAG TGCCCTGGAC 
AACCAGAGGA GCCCTTTGTC CCTGGGCAAA CGGGAGCCAC CATGGCTGGC CAGGCCCTGT 
CATGGGAACT GTGATCCGAC CCAGCAGGAG GCTGGGGGAG GGGAGACCGT GGTGACCTTC 
GAGGACCGGG CCAGGCACAG GCAGAGCCAG CGGCGCAGCC GGCACCGTCG TGTCAGGACG 
GAAGGCAAGG AGCTCTCCTC CACCTCCCGG AGCAGGTCTG TCAGCCAGGA GCGGAGTCTG 
GATGAGGGGG TGCTCACCGA CGGGGAGAAG GAGCGAGAGC CCAGGGGCGG CCATGCAGGC 
AAAGAACCCA CCATCCAAGA AGAAGACAGA GGCCAAGACT TAAGGAGGAC GAACAGTCTG 
ATGGTGCCCA GAGGCTCTGG GCTGGCAGGA GCCCTTGATG AGGCCAACAC ACCCCTAGTC 
CTGCCCCACC ACGATCTGGA AGTGGGGAAG GACACGGTGC TGACGGAGCA GGAGCCGGAG 
GGCAGCAGCG AGCAGGCCCT GCTGGGGGAT GTGCAGTTGG ACATTGGCAG GGTCATCAGC 
CAGAGTGAGC CTGACCTCTC CTGCGTCACA GCCAACACAG ACAAGGCCAC CACCGACAGC 
ACCAGCGTCA CCGTCGCCAT CCCTGATGTG GGCCCCTTGA TGGACTCAAC TGTGATTCAC 
ATTAGCAACA AAACTGATGG AGAAGCCAGT CCCTTGAAGG AGGCTGAGAT CAAAGAGGAG 
GAGGAGGAAA TGGAGAAGAA GCAGCAGAAG AAGGAGAAGC GTGAGACGGG CAAAGCCATG 
GTGCCCCACA GCTCTATGTT CATCTTCAGC ACCACCAACC CGATTCGCAG GGCCTGCCAC 
TACATCGTGA ACCTGCGCTA CTTTGAGATG TGCATTCTCC TGGTGATCGC CGCCAGCAGC 
ATTGCCCTGG CAGCAGAGGA CCCCGTCCTG ACCAACTCGG AGCGCAACAA AGTCCTGAGG 
TATTTTGACT ATGTTTTCAC TGGCGTGTTC ACCTTTGAGA TGGTTATAAA GATGATAGAC 
CAGGGCTTGA TCCTACAGGA TGGGTCCTAC TTCCGAGACC TGTGGAACAT CCTGGACTTT 
GTGGTGGTGG TTGGTGCATT GGTGGCCTTT GCTTTGGCAA CAAACAAGGG GCGAGACATC 
AAGACCATCA AATCCCTGAG GGTGCTCCGA GTTCTGAGGC CACTGAAGAC CATCAAGCGC 
TTGCCCAAAC TCAAGGCTGT CTTTGACTGT GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATCG TCTATAAGCT CTTCATGTTC ATCTTTGCTG TCATTGCGGT TCAGCTCTTC 
AAGGGAAAAT TCTTTTATTG CACAGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTACGTAG ATCATGAAAA AAACAAGATG GAGGTGAAAG GCCGGGAATG GAAGCGCCAT 
GAATTCCACT ACGACAACAT TATCTGGGCC CTGCTGACCC TCTTCACCGT CTCCACGGGG 
GAAGGATGGC CTCAAGTCTT ACAGCATTCT GTCGATGTGA CAGAGGAAGA CCGAGGACCA 
AGCCGCAGCA ACCGCATGGA GATGTCCATC TTCTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT TGTGGCTCTG ATCATCATCA CCTTCCAGGA ACAAGGGGAC 
AAGATGATGG AGGAATGCAG CCTGGAGAAG AACGAGAGGG CGTGCATTGA CTTCGCCATC 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGCGTC 
TGGCACTTCG TGGTGTCTCC GTCCTTTGAG TACACCATCA TGGCCATGAT CGCCCTGAAC 
ACCGTCGTGC TGATGATGAA GTACTACTCT GCTCCCTGTA CCTATGAACT GGCCCTGAAG 
TACCTGAATA TTGCCTTCAC CATGGTGTTT TCCCTGGAAT GTGTCCTAAA GATCATCGCT 
TTTGGCTTCT TGAACTACTT TCGAGACACC TGGAACATCT TTGACTTCAT CACTGTGATT 
GGCAGTATCA CAGAAATCAT ACTGACAGAC AGCAAGCTGG TAAACACCAG TGGTTTCAAC 
ATGAGCTTTC TGAAGCTCTT CCGTGCCGCC CGCCTCATCA AGCTTCTGCG GCAGGGCTAC 
ACCATCCGTA TTTTACTCTG GACCTTCGTG CAGTCCTTTA AGGCCCTCCC CTACGTCTGC 
CTTTTGATTG CCATGCTTTT CTTCATCTAT GCCATCATTG GGATGCAGGT ATTTGGAAAC 
ATAAAATTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGG 
TCCCTCATGT TACTCTTCAG GAGTGCCACG GGAGAGGCCT GGCAGGAGAT AATGCTGTCG 
TGCCTCGGGG AGAAAGGCTG CGAGCCCGAC ACCACTGCGC CATCGGGGCA GAACGAGAAT 
GAGCGCTGTG GCACCGATCT GGCCTATGTT TACTTTGTCT CCTTCATCTT CTTCTGCTCT 
TTCTTGATGC TCAACTTGTT TGTGGCCGTC ATCATGGACA ACTTCGAGTA CCTGACTCGG 
GACTCCTCCA TCTTAGGGCC CCACCACTTG GATGAGTTTG TCCGCGTCTG GGCAGAGTAC 
GACCGAGCTG CATGTGGCCG CATCCATTAT ACTGAGATGT ATGAAATGCT GACTCTCATG 
TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCATATAA GAGGTTGGTC 
CTAATGAATA TGCCAGTAGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTGATGGCT 
CTGATCCGGA CAGCCCTGGA CATCAAAATC GCCAAAGGTG GTGCAGACAG GCAGCAGCTA 
GACTCGGAGC TGCAAAAGGA GACCCTGGCC ATCTGGCCTC ACCTGTCCCA GAAGATGCTA 
GACCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAAAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTGAAGA AACAGAGGCA GCAGCTGGAG 
GAACAGAAAA ATGCACCCAT GTTCCAGCGA ATGGAGCCCT CATCTCTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCCTATCTC CAGCAGGACC CCGTTTCAGG CCTGAGTGGC 
CGTAGTGGAT ACCCTTCGAT GAGTCCACTC TCCCCCCAGG AAATATTCCA ATTGGCTTGT 
ATGGATCCAG CCGATGATGG ACAGTTCCAG GAACATCAGT CTCTGGAGCC AGAGGTTAGT 
GAATTAAAAA GCGTGCAGTC CTCTAACCAT GGCATCTACC TTCCTTCGGA CACCCAGGAG 
CATACGGGAT CTGGGAGGGC ATCTTCTATG CCACGCCTGA CTGTGGATCC CCAGGTGGTG 
ACAGATACTA GCTCCATGAG ACGTTCATTT TCCACTATTC GGGATAAGCG TTCAAATTCC 
TCCTGGTTGG AGGAATTCTC CATGGAGAGA AGCAGTGAAA ACACCTACAA GTCACGTCGT 
CGGAGTTACC ACTCCTCCCT GAGACTGTCA GCTCATCGCC TGAATTCTGA CTCAGGCCAC 
AAGTCTGACA CCCACCGCTC AGGGGGTAGA GAGCGGGGAC GATCGAAAGA GCGAAAGCAT 
CTTCTCTCTC CTGATGTCTC CCGCTGCAAC TCAGAGGAGC GAGGGACCCA GGCCGACTGG 
GAGTCCCCAG AGCGCCAACA GTCCAGGTCT CCCAGTGAGG CCAGGTCACA GACCCCCAAC 
AGACAGGGCA CGGGCTCCCT GAGCGAGAGC TCCATCCCCT CCATCTCTGA CACCAGCACC 
CCAAGACGAA GTCGCCGGCA GCTCCCACCC GTCCCACCAA AGCCTCGGCC CCTCCTCTCC 
TACAGCTCCC TGATTCGACA CTCCGGGAGC ATCTCCCCGC CGGCCGACGG AAGCGAGAGG 
GGCTCCCCGC TGACCCCCCA GGCGCTGGAG AGCACGGAGG CCGGCCTGGC GGAGCCCTCG 
GGCCCCCGGC ACGGGCACGG GCAGGGGCAC GGGCAGGGGC ACGGGCAGCA GAGCCAGCAC 
TGCTCCCCGC AGCGCTACAT CTCCGAGCCC TACCTGGCCC TGCACGAAGA CTCCCACGCG 
TCGGACTGTG GCGAGGAGGA GACGCTCACC TTCGAGGCCG CCGTGGCCAC CAGCCTGGGC 
CGCTCCAACA CCATCGGCTC GGCCCCGCCG CTGCGGCACA GCTGGCAGAT GCCCAACGGG 
CACTATCGGC GGCGGCGGCG CGGGGGGCCG GGGCCGGGCG TGATGTGCGG GGCGGTCAGC 
GACCTCCTGA GTGACACGGA AGAAGACGAC AAGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970590.1
CDS	86..7504
Translation	MEEQCSGMVPEMRGPSPGRRLPEPAPCPAAAGSPEPWAAGRRRHVLALPEEEAWRPQQCSLLEADASDEVGMLPASPRAAASGVDNFFPVQEGEGAGWEGGAALDAGAGPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFTNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLGLGLEKCEEERIISRGGSLKGDGGDLSSALDNQRSPLSLGKREPPWLARPCHGNCDPTQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKELSSTSRSRSVSQERSLDEGVLTDGEKEREPRGGHAGKEPTIQEEDRGQDLRRTNSLMVPRGSGLAGALDEANTPLVLPHHDLEVGKDTVLTEQEPEGSSEQALLGDVQLDIGRVISQSEPDLSCVTANTDKATTDSTSVTVAIPDVGPLMDSTVIHISNKTDGEASPLKEAEIKEEEEEMEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALATNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEHQSLEPEVSELKSVQSSNHGIYLPSDTQEHTGSGRASSMPRLTVDPQVVTDTSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERQQSRSPSEARSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHSGSISPPADGSERGSPLTPQALESTEAGLAEPSGPRHGHGQGHGQGHGQQSQHCSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGVMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_014115115.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115115.1

ORF Insert Sequence:

ATGGAAGAGC AATGTTCTGG GATGGTGCCC GAGATGCGTG GACCGAGCCC AGGCCGCAGG 
CTGCCCGAGC CCGCCCCCTG CCCCGCCGCG GCCGGGAGCC CTGAGCCCTG GGCCGCGGGC 
CGCCGCCGCC ACGTCCTGGC CCTGCCTGAG GAGGAGGCAT GGAGGCCCCA GCAGTGCTCC 
CTGCTCGAGG CCGACGCCTC GGACGAGGTG GGCATGCTGC CGGCCTCCCC GCGCGCCGCC 
GCCTCCGGCG TCGACAACTT CTTCCCGGTG CAGGAGGGCG AGGGCGCGGG CTGGGAGGGC 
GGTGCGGCCC TGGACGCCGG CGCCGGCCCC TTCCTCCCGG TGAGCCCCGA GGTGATGAAG 
CGGCGGCGCG GGGGCCTCAT CGAGCAGCGC GACATCATCA AGGCCCACGA GGCGCACAAG 
ATGCAGAGCA CCCCGCAGGC GCGGCGCAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG 
GTGGTCGGCA GGCCGGGGTC GGGCGATGGA GACTCGGACC AGAGCAGGAA CCGGCAAGGA 
ACCCCGGTGC CGGCCTCGGG GCCGGCGGCC GCCTACAAGC AGTCGAAAGC GCAGAGGGCG 
CGGACTATGG CTTTGTACAA CCCCATTCCC GTCCGGCAGA ACTGTTTCAC CGTCAACAGA 
TCCCTGTTCA TCTTCGGAGA AGATAACATT GTCAGGAAAT ACGCCAAGAA GCTCATCGAC 
TGGCCGCCGT TTGAGTACAT GATTTTGGCA ACCATCATTG CCAACTGCAT TGTCTTGGCC 
CTGGAACAGC ATCTTCCTGA GGATGACAAG ACCCCGATGT CCCGCAGACT GGAGAAGACA 
GAGCCGTATT TCATTGGGAT CTTTTGCTTT GAAGCCGGTA TCAAAATTGT GGCCCTGGGG 
TTCATCTTTC ATAAGGGCTC GTACCTCCGC AATGGCTGGA ATGTCATGGA CTTCATCGTG 
GTCCTCAGTG GCATCCTGGC CACTGCGGGG ACTCACTTCA ACACACACGT GGACCTGAGG 
ACCCTCCGGG CTGTGCGTGT CCTGAGACCC CTGAAGCTCG TGTCAGGGAT TCCTAGCCTG 
CAGATCGTGT TGAAGTCTAT CATGAAGGCC ATGGTGCCTC TTCTGCAAAT TGGTCTCCTG 
CTCTTCTTTG CCATCTTGAT GTTTGCTATC ATTGGCTTAG AGTTCTACAG TGGGAAGTTG 
CACCGAGCAT GTTTCACGAA CAATTCAGGT ATTCTGGAAG GATTTGACCC CCCTCACCCA 
TGTGGTGTGC AGGGCTGCCC AGCTGGCTAC GAATGCCGGG ACTGGATTGG CCCCAATGAT 
GGGATCACCC AGTTTGATAA CATCCTTTTT GCTGTGCTGA CTGTCTTCCA ATGCATCACC 
ATGGAAGGAT GGACCACCGT GCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC 
TGGCTGTACT TCATCCCCCT CATCATCATT GGATCCTTCT TTGTTCTCAA CCTAGTTCTG 
GGAGTGCTTT CCGGGGAGTT TGCCAAAGAG AGAGAGAGAG TGGAGAACCG GAGAGCTTTC 
ATGAAACTGC GGCGGCAGCA GCAGATTGAG CGGGAGCTGA ACGGGTACCG CGCCTGGATA 
GACAAAGCAG AGGAAGTCAT GCTTGCTGAA GAAAATAAAA ATGCTGGGAC GTCAGCCTTA 
GAAGTGCTTC GAAGGGCAAC CATCAAAAGG AGCCGGACAG AGGCCATGAC TCGAGACTCC 
AGTGATGAAC ACTGTGTCGA TATCTCCTCT GTGGGCACAC CTCTCGCCCG AGCCAGCATC 
AAAAGTGCAA AGGTGGATGG GGCCTCCTAT TTCCGGCACA AAGAAAGGCT TCTGCGCATC 
TCTATCCGCC ACATGGTGAA ATCCCAGGTG TTTTACTGGA TTGTGTTGAG CCTTGTGGCT 
CTCAACACTG CCTGTGTGGC CATCGTCCAT CATAACCAGC CCCAGTGGCT CACCCATCTC 
CTCTACTATG CAGAATTTTT GTTTCTGGGA CTGTTCCTCT TGGAGATGTC CCTGAAGATG 
TATGGCATGG GACCTCGACT TTATTTTCAC TCTTCATTCA ACTGTTTTGA TTTTGGGGTC 
ACAGTGGGCA GCATCTTTGA AGTGGTCTGG GCAATCTTCA GACCTGGCAC ATCTTTTGGA 
ATCAGTGTCT TACGGGCTCT CCGGCTTCTA AGAATATTTA AAATAACCAA GTATTGGGCA 
TCTCTGCGAA ATTTGGTGGT CTCCTTGATG AGCTCTATGA AGTCTATCAT CAGTTTGCTC 
TTCCTCCTCT TCCTCTTCAT CGTTGTCTTT GCTCTCCTAG GAATGCAGCT GTTTGGAGGC 
AGGTTTAACT TTAATGATGG GACTCCTTCA GCAAACTTTG ATACCTTCCC TGCAGCCATC 
ATGACTGTAT TCCAGATCCT AACAGGTGAG GACTGGAATG AGGTGATGTA CAATGGGATC 
CGTTCCCAGG GTGGAGTTAG CTCAGGCATG TGGTCAGCTA TCTATTTCAT TGTGCTCACC 
TTGTTTGGAA ACTACACACT GCTGAATGTG TTCTTGGCCA TTGCTGTGGA CAATCTGGCC 
AATGCCCAAG AGCTGACCAA GGATGAACAA GAAGAAGAGG AGGCTTTTAA CCAGAAACAT 
GCATTGCAGA AGGCCAAGGA GGTCAGCCCG ATGTCTGCAC CCAACATGCC TTCTATCGAA 
AGAGACAGAA GGAGAAGACA CCACATGTCG ATGTGGGAGC CACGCAGCAG CCACCTGAGG 
GAGCGGAGGC GCCGGCACCA CATGTCGGTG TGGGAGCAGC GCACCAGCCA GCTGAGAAGG 
CACATGCAGA TGTCCAGCCA GGAGGCCCTC AATAAAGAGG AGGCCCCACC GATGAACCCC 
CTCAACCCCC TCAACCCGCT GAGCCCCCTC AACCCGCTCA ACGCCCACCC CAGCCTCTAC 
CGGAGACCTA GGCCTATTGA GGGCCTGGGC CTGGGCCTGG AGAAGTGTGA GGAGGAGCGC 
ATCATCAGCC GCGGGGGGTC CCTCAAGGGG GACGGAGGGG ACCTGTCGAG TGCCCTGGAC 
AACCAGAGGA GCCCTTTGTC CCTGGGCAAA CGGGAGCCAC CATGGCTGGC CAGGCCCTGT 
CATGGGAACT GTGATCCGAC CCAGCAGGAG GCTGGGGGAG GGGAGACCGT GGTGACCTTC 
GAGGACCGGG CCAGGCACAG GCAGAGCCAG CGGCGCAGCC GGCACCGTCG TGTCAGGACG 
GAAGGCAAGG AGCTCTCCTC CACCTCCCGG AGCAGGTCTG TCAGCCAGGA GCGGAGTCTG 
GATGAGGGGG TGCTCACCGA CGGGGAGAAG GAGCGAGAGC CCAGGGGCGG CCATGCAGGC 
AAAGAACCCA CCATCCAAGA AGAAGACAGA GGCCAAGACT TAAGGAGGAC GAACAGTCTG 
ATGGTGCCCA GAGGCTCTGG GCTGGCAGGA GCCCTTGATG AGGCCAACAC ACCCCTAGTC 
CTGCCCCACC ACGATCTGGA AGTGGGGAAG GACACGGTGC TGACGGAGCA GGAGCCGGAG 
GGCAGCAGCG AGCAGGCCCT GCTGGGGGAT GTGCAGTTGG ACATTGGCAG GGTCATCAGC 
CAGAGTGAGC CTGACCTCTC CTGCGTCACA GCCAACACAG ACAAGGCCAC CACCGACAGC 
ACCAGCGTCA CCGTCGCCAT CCCTGATGTG GGCCCCTTGA TGGACTCAAC TGTGATTCAC 
ATTAGCAACA AAACTGATGG AGAAGCCAGT CCCTTGAAGG AGGCTGAGAT CAAAGAGGAG 
GAGGAGGAAA TGGAGAAGAA GCAGCAGAAG AAGGAGAAGC GTGAGACGGG CAAAGCCATG 
GTGCCCCACA GCTCTATGTT CATCTTCAGC ACCACCAACC CGATTCGCAG GGCCTGCCAC 
TACATCGTGA ACCTGCGCTA CTTTGAGATG TGCATTCTCC TGGTGATCGC CGCCAGCAGC 
ATTGCCCTGG CAGCAGAGGA CCCCGTCCTG ACCAACTCGG AGCGCAACAA AGTCCTGAGG 
TATTTTGACT ATGTTTTCAC TGGCGTGTTC ACCTTTGAGA TGGTTATAAA GATGATAGAC 
CAGGGCTTGA TCCTACAGGA TGGGTCCTAC TTCCGAGACC TGTGGAACAT CCTGGACTTT 
GTGGTGGTGG TTGGTGCATT GGTGGCCTTT GCTTTGGCAA CAAACAAGGG GCGAGACATC 
AAGACCATCA AATCCCTGAG GGTGCTCCGA GTTCTGAGGC CACTGAAGAC CATCAAGCGC 
TTGCCCAAAC TCAAGGCTGT CTTTGACTGT GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATCG TCTATAAGCT CTTCATGTTC ATCTTTGCTG TCATTGCGGT TCAGCTCTTC 
AAGGGAAAAT TCTTTTATTG CACAGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTACGTAG ATCATGAAAA AAACAAGATG GAGGTGAAAG GCCGGGAATG GAAGCGCCAT 
GAATTCCACT ACGACAACAT TATCTGGGCC CTGCTGACCC TCTTCACCGT CTCCACGGGG 
GAAGGATGGC CTCAAGTCTT ACAGCATTCT GTCGATGTGA CAGAGGAAGA CCGAGGACCA 
AGCCGCAGCA ACCGCATGGA GATGTCCATC TTCTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT TGTGGCTCTG ATCATCATCA CCTTCCAGGA ACAAGGGGAC 
AAGATGATGG AGGAATGCAG CCTGGAGAAG AACGAGAGGG CGTGCATTGA CTTCGCCATC 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGCGTC 
TGGCACTTCG TGGTGTCTCC GTCCTTTGAG TACACCATCA TGGCCATGAT CGCCCTGAAC 
ACCGTCGTGC TGATGATGAA GTACTACTCT GCTCCCTGTA CCTATGAACT GGCCCTGAAG 
TACCTGAATA TTGCCTTCAC CATGGTGTTT TCCCTGGAAT GTGTCCTAAA GATCATCGCT 
TTTGGCTTCT TGAACTACTT TCGAGACACC TGGAACATCT TTGACTTCAT CACTGTGATT 
GGCAGTATCA CAGAAATCAT ACTGACAGAC AGCAAGCTGG TAAACACCAG TGGTTTCAAC 
ATGAGCTTTC TGAAGCTCTT CCGTGCCGCC CGCCTCATCA AGCTTCTGCG GCAGGGCTAC 
ACCATCCGTA TTTTACTCTG GACCTTCGTG CAGTCCTTTA AGGCCCTCCC CTACGTCTGC 
CTTTTGATTG CCATGCTTTT CTTCATCTAT GCCATCATTG GGATGCAGGT ATTTGGAAAC 
ATAAAATTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGG 
TCCCTCATGT TACTCTTCAG GAGTGCCACG GGAGAGGCCT GGCAGGAGAT AATGCTGTCG 
TGCCTCGGGG AGAAAGGCTG CGAGCCCGAC ACCACTGCGC CATCGGGGCA GAACGAGAAT 
GAGCGCTGTG GCACCGATCT GGCCTATGTT TACTTTGTCT CCTTCATCTT CTTCTGCTCT 
TTCTTGATGC TCAACTTGTT TGTGGCCGTC ATCATGGACA ACTTCGAGTA CCTGACTCGG 
GACTCCTCCA TCTTAGGGCC CCACCACTTG GATGAGTTTG TCCGCGTCTG GGCAGAGTAC 
GACCGAGCTG CATGTGGCCG CATCCATTAT ACTGAGATGT ATGAAATGCT GACTCTCATG 
TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCATATAA GAGGTTGGTC 
CTAATGAATA TGCCAGTAGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTGATGGCT 
CTGATCCGGA CAGCCCTGGA CATCAAAATC GCCAAAGGTG GTGCAGACAG GCAGCAGCTA 
GACTCGGAGC TGCAAAAGGA GACCCTGGCC ATCTGGCCTC ACCTGTCCCA GAAGATGCTA 
GACCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAAAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTGAAGA AACAGAGGCA GCAGCTGGAG 
GAACAGAAAA ATGCACCCAT GTTCCAGCGA ATGGAGCCCT CATCTCTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCCTATCTC CAGCAGGACC CCGTTTCAGG CCTGAGTGGC 
CGTAGTGGAT ACCCTTCGAT GAGTCCACTC TCCCCCCAGG AAATATTCCA ATTGGCTTGT 
ATGGATCCAG CCGATGATGG ACAGTTCCAG GAACATCAGT CTCTGGAGCC AGAGGTTAGT 
GAATTAAAAA GCGTGCAGTC CTCTAACCAT GGCATCTACC TTCCTTCGGA CACCCAGGAG 
CATACGGGAT CTGGGAGGGC ATCTTCTATG CCACGCCTGA CTGTGGATCC CCAGGTGGTG 
ACAGATACTA GCTCCATGAG ACGTTCATTT TCCACTATTC GGGATAAGCG TTCAAATTCC 
TCCTGGTTGG AGGAATTCTC CATGGAGAGA AGCAGTGAAA ACACCTACAA GTCACGTCGT 
CGGAGTTACC ACTCCTCCCT GAGACTGTCA GCTCATCGCC TGAATTCTGA CTCAGGCCAC 
AAGTCTGACA CCCACCGCTC AGGGGGTAGA GAGCGGGGAC GATCGAAAGA GCGAAAGCAT 
CTTCTCTCTC CTGATGTCTC CCGCTGCAAC TCAGAGGAGC GAGGGACCCA GGCCGACTGG 
GAGTCCCCAG AGCGCCAACA GTCCAGGTCT CCCAGTGAGG CCAGGTCACA GACCCCCAAC 
AGACAGGGCA CGGGCTCCCT GAGCGAGAGC TCCATCCCCT CCATCTCTGA CACCAGCACC 
CCAAGACGAA GTCGCCGGCA GCTCCCACCC GTCCCACCAA AGCCTCGGCC CCTCCTCTCC 
TACAGCTCCC TGATTCGACA CTCCGGGAGC ATCTCCCCGC CGGCCGACGG AAGCGAGAGG 
GGCTCCCCGC TGACCCCCCA GGCGCTGGAG AGCACGGAGG CCGGCCTGGC GGAGCCCTCG 
GGCCCCCGGC ACGGGCACGG GCAGGGGCAC GGGCAGGGGC ACGGGCAGCA GAGCCAGCAC 
TGCTCCCCGC AGCGCTACAT CTCCGAGCCC TACCTGGCCC TGCACGAAGA CTCCCACGCG 
TCGGACTGTG GCGAGGAGGA GACGCTCACC TTCGAGGCCG CCGTGGCCAC CAGCCTGGGC 
CGCTCCAACA CCATCGGCTC GGCCCCGCCG CTGCGGCACA GCTGGCAGAT GCCCAACGGG 
CACTATCGGC GGCGGCGGCG CGGGGGGCCG GGGCCGGGCG TGATGTGCGG GGCGGTCAGC 
GACCTCCTGA GTGACACGGA AGAAGACGAC AAGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_014115116.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970591.1 Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OCe37412
Clone ID Related Accession (Same CDS sequence)	XM_014115116.1
Accession Version	XM_014115116.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGC AATGTTCTGG GATGGTGCCC GAGATGCGTG GACCGAGCCC AGGCCGCAGG 
CTGCCCGAGC CCGCCCCCTG CCCCGCCGCG GCCGGGAGCC CTGAGCCCTG GGCCGCGGGC 
CGCCGCCGCC ACGTCCTGGC CCTGCCTGAG GAGGAGGCAT GGAGGCCCCA GCAGTGCTCC 
CTGCTCGAGG CCGACGCCTC GGACGAGGTG GGCATGCTGC CGGCCTCCCC GCGCGCCGCC 
GCCTCCGGCG TCGACAACTT CTTCCCGGTG CAGGAGGGCG AGGGCGCGGG CTGGGAGGGC 
GGTGCGGCCC TGGACGCCGG CGCCGGCCCC TTCCTCCCGG TGAGCCCCGA GGTGATGAAG 
CGGCGGCGCG GGGGCCTCAT CGAGCAGCGC GACATCATCA AGGCCCACGA GGCGCACAAG 
ATGCAGAGCA CCCCGCAGGC GCGGCGCAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG 
GTGGTCGGCA GGCCGGGGTC GGGCGATGGA GACTCGGACC AGAGCAGGAA CCGGCAAGGA 
ACCCCGGTGC CGGCCTCGGG GCCGGCGGCC GCCTACAAGC AGTCGAAAGC GCAGAGGGCG 
CGGACTATGG CTTTGTACAA CCCCATTCCC GTCCGGCAGA ACTGTTTCAC CGTCAACAGA 
TCCCTGTTCA TCTTCGGAGA AGATAACATT GTCAGGAAAT ACGCCAAGAA GCTCATCGAC 
TGGCCGCCGT TTGAGTACAT GATTTTGGCA ACCATCATTG CCAACTGCAT TGTCTTGGCC 
CTGGAACAGC ATCTTCCTGA GGATGACAAG ACCCCGATGT CCCGCAGACT GGAGAAGACA 
GAGCCGTATT TCATTGGGAT CTTTTGCTTT GAAGCCGGTA TCAAAATTGT GGCCCTGGGG 
TTCATCTTTC ATAAGGGCTC GTACCTCCGC AATGGCTGGA ATGTCATGGA CTTCATCGTG 
GTCCTCAGTG GCATCCTGGC CACTGCGGGG ACTCACTTCA ACACACACGT GGACCTGAGG 
ACCCTCCGGG CTGTGCGTGT CCTGAGACCC CTGAAGCTCG TGTCAGGGAT TCCTAGCCTG 
CAGATCGTGT TGAAGTCTAT CATGAAGGCC ATGGTGCCTC TTCTGCAAAT TGGTCTCCTG 
CTCTTCTTTG CCATCTTGAT GTTTGCTATC ATTGGCTTAG AGTTCTACAG TGGGAAGTTG 
CACCGAGCAT GTTTCACGAA CAATTCAGGT ATTCTGGAAG GATTTGACCC CCCTCACCCA 
TGTGGTGTGC AGGGCTGCCC AGCTGGCTAC GAATGCCGGG ACTGGATTGG CCCCAATGAT 
GGGATCACCC AGTTTGATAA CATCCTTTTT GCTGTGCTGA CTGTCTTCCA ATGCATCACC 
ATGGAAGGAT GGACCACCGT GCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC 
TGGCTGTACT TCATCCCCCT CATCATCATT GGATCCTTCT TTGTTCTCAA CCTAGTTCTG 
GGAGTGCTTT CCGGGGAGTT TGCCAAAGAG AGAGAGAGAG TGGAGAACCG GAGAGCTTTC 
ATGAAACTGC GGCGGCAGCA GCAGATTGAG CGGGAGCTGA ACGGGTACCG CGCCTGGATA 
GACAAAGCAG AGGAAGTCAT GCTTGCTGAA GAAAATAAAA ATGCTGGGAC GTCAGCCTTA 
GAAGTGCTTC GAAGGGCAAC CATCAAAAGG AGCCGGACAG AGGCCATGAC TCGAGACTCC 
AGTGATGAAC ACTGTGTCGA TATCTCCTCT GTGGGCACAC CTCTCGCCCG AGCCAGCATC 
AAAAGTGCAA AGGTGGATGG GGCCTCCTAT TTCCGGCACA AAGAAAGGCT TCTGCGCATC 
TCTATCCGCC ACATGGTGAA ATCCCAGGTG TTTTACTGGA TTGTGTTGAG CCTTGTGGCT 
CTCAACACTG CCTGTGTGGC CATCGTCCAT CATAACCAGC CCCAGTGGCT CACCCATCTC 
CTCTACTATG CAGAATTTTT GTTTCTGGGA CTGTTCCTCT TGGAGATGTC CCTGAAGATG 
TATGGCATGG GACCTCGACT TTATTTTCAC TCTTCATTCA ACTGTTTTGA TTTTGGGGTC 
ACAGTGGGCA GCATCTTTGA AGTGGTCTGG GCAATCTTCA GACCTGGCAC ATCTTTTGGA 
ATCAGTGTCT TACGGGCTCT CCGGCTTCTA AGAATATTTA AAATAACCAA GTATTGGGCA 
TCTCTGCGAA ATTTGGTGGT CTCCTTGATG AGCTCTATGA AGTCTATCAT CAGTTTGCTC 
TTCCTCCTCT TCCTCTTCAT CGTTGTCTTT GCTCTCCTAG GAATGCAGCT GTTTGGAGGC 
AGGTTTAACT TTAATGATGG GACTCCTTCA GCAAACTTTG ATACCTTCCC TGCAGCCATC 
ATGACTGTAT TCCAGATCCT AACAGGTGAG GACTGGAATG AGGTGATGTA CAATGGGATC 
CGTTCCCAGG GTGGAGTTAG CTCAGGCATG TGGTCAGCTA TCTATTTCAT TGTGCTCACC 
TTGTTTGGAA ACTACACACT GCTGAATGTG TTCTTGGCCA TTGCTGTGGA CAATCTGGCC 
AATGCCCAAG AGCTGACCAA GGATGAACAA GAAGAAGAGG AGGCTTTTAA CCAGAAACAT 
GCATTGCAGA AGGCCAAGGA GGTCAGCCCG ATGTCTGCAC CCAACATGCC TTCTATCGAG 
AGGGAGCGGA GGCGCCGGCA CCACATGTCG GTGTGGGAGC AGCGCACCAG CCAGCTGAGA 
AGGCACATGC AGATGTCCAG CCAGGAGGCC CTCAATAAAG AGGAGGCCCC ACCGATGAAC 
CCCCTCAACC CCCTCAACCC GCTGAGCCCC CTCAACCCGC TCAACGCCCA CCCCAGCCTC 
TACCGGAGAC CTAGGCCTAT TGAGGGCCTG GGCCTGGGCC TGGAGAAGTG TGAGGAGGAG 
CGCATCATCA GCCGCGGGGG GTCCCTCAAG GGGGACGGAG GGGACCTGTC GAGTGCCCTG 
GACAACCAGA GGAGCCCTTT GTCCCTGGGC AAACGGGAGC CACCATGGCT GGCCAGGCCC 
TGTCATGGGA ACTGTGATCC GACCCAGCAG GAGGCTGGGG GAGGGGAGAC CGTGGTGACC 
TTCGAGGACC GGGCCAGGCA CAGGCAGAGC CAGCGGCGCA GCCGGCACCG TCGTGTCAGG 
ACGGAAGGCA AGGAGCTCTC CTCCACCTCC CGGAGCAGGT CTGTCAGCCA GGAGCGGAGT 
CTGGATGAGG GGGTGCTCAC CGACGGGGAG AAGGAGCGAG AGCCCAGGGG CGGCCATGCA 
GGCAAAGAAC CCACCATCCA AGAAGAAGAC AGAGGCCAAG ACTTAAGGAG GACGAACAGT 
CTGATGGTGC CCAGAGGCTC TGGGCTGGCA GGAGCCCTTG ATGAGGCCAA CACACCCCTA 
GTCCTGCCCC ACCACGATCT GGAAGTGGGG AAGGACACGG TGCTGACGGA GCAGGAGCCG 
GAGGGCAGCA GCGAGCAGGC CCTGCTGGGG GATGTGCAGT TGGACATTGG CAGGGTCATC 
AGCCAGAGTG AGCCTGACCT CTCCTGCGTC ACAGCCAACA CAGACAAGGC CACCACCGAC 
AGCACCAGCG TCACCGTCGC CATCCCTGAT GTGGGCCCCT TGATGGACTC AACTGTGATT 
CACATTAGCA ACAAAACTGA TGGAGAAGCC AGTCCCTTGA AGGAGGCTGA GATCAAAGAG 
GAGGAGGAGG AAATGGAGAA GAAGCAGCAG AAGAAGGAGA AGCGTGAGAC GGGCAAAGCC 
ATGGTGCCCC ACAGCTCTAT GTTCATCTTC AGCACCACCA ACCCGATTCG CAGGGCCTGC 
CACTACATCG TGAACCTGCG CTACTTTGAG ATGTGCATTC TCCTGGTGAT CGCCGCCAGC 
AGCATTGCCC TGGCAGCAGA GGACCCCGTC CTGACCAACT CGGAGCGCAA CAAAGTCCTG 
AGGTATTTTG ACTATGTTTT CACTGGCGTG TTCACCTTTG AGATGGTTAT AAAGATGATA 
GACCAGGGCT TGATCCTACA GGATGGGTCC TACTTCCGAG ACCTGTGGAA CATCCTGGAC 
TTTGTGGTGG TGGTTGGTGC ATTGGTGGCC TTTGCTTTGG CGAACGCTTT GGGAACAAAC 
AAGGGGCGAG ACATCAAGAC CATCAAATCC CTGAGGGTGC TCCGAGTTCT GAGGCCACTG 
AAGACCATCA AGCGCTTGCC CAAACTCAAG GCTGTCTTTG ACTGTGTGGT GACCTCCTTG 
AAGAATGTCT TCAACATACT CATCGTCTAT AAGCTCTTCA TGTTCATCTT TGCTGTCATT 
GCGGTTCAGC TCTTCAAGGG AAAATTCTTT TATTGCACAG ACAGTTCCAA GGACACAGAG 
AAGGAGTGCA TAGGCAACTA CGTAGATCAT GAAAAAAACA AGATGGAGGT GAAAGGCCGG 
GAATGGAAGC GCCATGAATT CCACTACGAC AACATTATCT GGGCCCTGCT GACCCTCTTC 
ACCGTCTCCA CGGGGGAAGG ATGGCCTCAA GTCTTACAGC ATTCTGTCGA TGTGACAGAG 
GAAGACCGAG GACCAAGCCG CAGCAACCGC ATGGAGATGT CCATCTTCTA TGTGGTCTAC 
TTTGTGGTCT TCCCTTTCTT CTTTGTCAAT ATCTTTGTGG CTCTGATCAT CATCACCTTC 
CAGGAACAAG GGGACAAGAT GATGGAGGAA TGCAGCCTGG AGAAGAACGA GAGGGCGTGC 
ATTGACTTCG CCATCAGTGC CAAACCTCTC ACCCGCTACA TGCCACAGAA CAGGCACACC 
TTCCAGTACC GCGTCTGGCA CTTCGTGGTG TCTCCGTCCT TTGAGTACAC CATCATGGCC 
ATGATCGCCC TGAACACCGT CGTGCTGATG ATGAAGTACT ACTCTGCTCC CTGTACCTAT 
GAACTGGCCC TGAAGTACCT GAATATTGCC TTCACCATGG TGTTTTCCCT GGAATGTGTC 
CTAAAGATCA TCGCTTTTGG CTTCTTGAAC TACTTTCGAG ACACCTGGAA CATCTTTGAC 
TTCATCACTG TGATTGGCAG TATCACAGAA ATCATACTGA CAGACAGCAA GCTGGTAAAC 
ACCAGTGGTT TCAACATGAG CTTTCTGAAG CTCTTCCGTG CCGCCCGCCT CATCAAGCTT 
CTGCGGCAGG GCTACACCAT CCGTATTTTA CTCTGGACCT TCGTGCAGTC CTTTAAGGCC 
CTCCCCTACG TCTGCCTTTT GATTGCCATG CTTTTCTTCA TCTATGCCAT CATTGGGATG 
CAGGTATTTG GAAACATAAA ATTAGATGAG GAGAGTCACA TCAACCGGCA CAACAACTTC 
CGGAGTTTCT TTGGGTCCCT CATGTTACTC TTCAGGAGTG CCACGGGAGA GGCCTGGCAG 
GAGATAATGC TGTCGTGCCT CGGGGAGAAA GGCTGCGAGC CCGACACCAC TGCGCCATCG 
GGGCAGAACG AGAATGAGCG CTGTGGCACC GATCTGGCCT ATGTTTACTT TGTCTCCTTC 
ATCTTCTTCT GCTCTTTCTT GATGCTCAAC TTGTTTGTGG CCGTCATCAT GGACAACTTC 
GAGTACCTGA CTCGGGACTC CTCCATCTTA GGGCCCCACC ACTTGGATGA GTTTGTCCGC 
GTCTGGGCAG AGTACGACCG AGCTGCATGC GGCAGGATCC CGTATAAGGA TATGTACAAA 
TTAGTGCGGG TGATCTCGCC GCCTCTGGGC CTGGGAGAGA ACTGCCCCTA CAGGGTGGCT 
TGCAAGAGGT TGGTCCTAAT GAATATGCCA GTAGCTGAGG ACATGACAGT CCACTTCACC 
TCCACACTGA TGGCTCTGAT CCGGACAGCC CTGGACATCA AAATCGCCAA AGGTGGTGCA 
GACAGGCAGC AGCTAGACTC GGAGCTGCAA AAGGAGACCC TGGCCATCTG GCCTCACCTG 
TCCCAGAAGA TGCTAGACCT GCTTGTGCCC ATGCCCAAAG CCTCTGACCT GACTGTGGGC 
AAAATCTATG CAGCAATGAT GATCATGGAC TACTATAAAC AGAGTAAGGT GAAGAAACAG 
AGGCAGCAGC TGGAGGAACA GAAAAATGCA CCCATGTTCC AGCGAATGGA GCCCTCATCT 
CTGCCTCAGG AGATCATTGC TAATGCCAAA GCCCTGCCCT ATCTCCAGCA GGACCCCGTT 
TCAGGCCTGA GTGGCCGTAG TGGATACCCT TCGATGAGTC CACTCTCCCC CCAGGAAATA 
TTCCAATTGG CTTGTATGGA TCCAGCCGAT GATGGACAGT TCCAGGAACA TCAGTCTCTG 
GTGGTGACAG ATACTAGCTC CATGAGACGT TCATTTTCCA CTATTCGGGA TAAGCGTTCA 
AATTCCTCCT GGTTGGAGGA ATTCTCCATG GAGAGAAGCA GTGAAAACAC CTACAAGTCA 
CGTCGTCGGA GTTACCACTC CTCCCTGAGA CTGTCAGCTC ATCGCCTGAA TTCTGACTCA 
GGCCACAAGT CTGACACCCA CCGCTCAGGG GGTAGAGAGC GGGGACGATC GAAAGAGCGA 
AAGCATCTTC TCTCTCCTGA TGTCTCCCGC TGCAACTCAG AGGAGCGAGG GACCCAGGCC 
GACTGGGAGT CCCCAGAGCG CCAACAGTCC AGGTCTCCCA GTGAGGCCAG GTCACAGACC 
CCCAACAGAC AGGGCACGGG CTCCCTGAGC GAGAGCTCCA TCCCCTCCAT CTCTGACACC 
AGCACCCCAA GACGAAGTCG CCGGCAGCTC CCACCCGTCC CACCAAAGCC TCGGCCCCTC 
CTCTCCTACA GCTCCCTGAT TCGACACTCC GGGAGCATCT CCCCGCCGGC CGACGGAAGC 
GAGAGGGGCT CCCCGCTGAC CCCCCAGGCG CTGGAGAGCA CGGAGGCCGG CCTGGCGGAG 
CCCTCGGGCC CCCGGCACGG GCACGGGCAG GGGCACGGGC AGGGGCACGG GCAGCAGAGC 
CAGCACTGCT CCCCGCAGCG CTACATCTCC GAGCCCTACC TGGCCCTGCA CGAAGACTCC 
CACGCGTCGG ACTGTGGCGA GGAGGAGACG CTCACCTTCG AGGCCGCCGT GGCCACCAGC 
CTGGGCCGCT CCAACACCAT CGGCTCGGCC CCGCCGCTGC GGCACAGCTG GCAGATGCCC 
AACGGGCACT ATCGGCGGCG GCGGCGCGGG GGGCCGGGGC CGGGCGTGAT GTGCGGGGCG 
GTCAGCGACC TCCTGAGTGA CACGGAAGAA GACGACAAGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970591.1
CDS	86..7330
Translation	MEEQCSGMVPEMRGPSPGRRLPEPAPCPAAAGSPEPWAAGRRRHVLALPEEEAWRPQQCSLLEADASDEVGMLPASPRAAASGVDNFFPVQEGEGAGWEGGAALDAGAGPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFTNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLGLGLEKCEEERIISRGGSLKGDGGDLSSALDNQRSPLSLGKREPPWLARPCHGNCDPTQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKELSSTSRSRSVSQERSLDEGVLTDGEKEREPRGGHAGKEPTIQEEDRGQDLRRTNSLMVPRGSGLAGALDEANTPLVLPHHDLEVGKDTVLTEQEPEGSSEQALLGDVQLDIGRVISQSEPDLSCVTANTDKATTDSTSVTVAIPDVGPLMDSTVIHISNKTDGEASPLKEAEIKEEEEEMEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIPYKDMYKLVRVISPPLGLGENCPYRVACKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEHQSLVVTDTSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERQQSRSPSEARSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHSGSISPPADGSERGSPLTPQALESTEAGLAEPSGPRHGHGQGHGQGHGQQSQHCSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGVMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_014115116.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115116.1

ORF Insert Sequence:

ATGGAAGAGC AATGTTCTGG GATGGTGCCC GAGATGCGTG GACCGAGCCC AGGCCGCAGG 
CTGCCCGAGC CCGCCCCCTG CCCCGCCGCG GCCGGGAGCC CTGAGCCCTG GGCCGCGGGC 
CGCCGCCGCC ACGTCCTGGC CCTGCCTGAG GAGGAGGCAT GGAGGCCCCA GCAGTGCTCC 
CTGCTCGAGG CCGACGCCTC GGACGAGGTG GGCATGCTGC CGGCCTCCCC GCGCGCCGCC 
GCCTCCGGCG TCGACAACTT CTTCCCGGTG CAGGAGGGCG AGGGCGCGGG CTGGGAGGGC 
GGTGCGGCCC TGGACGCCGG CGCCGGCCCC TTCCTCCCGG TGAGCCCCGA GGTGATGAAG 
CGGCGGCGCG GGGGCCTCAT CGAGCAGCGC GACATCATCA AGGCCCACGA GGCGCACAAG 
ATGCAGAGCA CCCCGCAGGC GCGGCGCAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG 
GTGGTCGGCA GGCCGGGGTC GGGCGATGGA GACTCGGACC AGAGCAGGAA CCGGCAAGGA 
ACCCCGGTGC CGGCCTCGGG GCCGGCGGCC GCCTACAAGC AGTCGAAAGC GCAGAGGGCG 
CGGACTATGG CTTTGTACAA CCCCATTCCC GTCCGGCAGA ACTGTTTCAC CGTCAACAGA 
TCCCTGTTCA TCTTCGGAGA AGATAACATT GTCAGGAAAT ACGCCAAGAA GCTCATCGAC 
TGGCCGCCGT TTGAGTACAT GATTTTGGCA ACCATCATTG CCAACTGCAT TGTCTTGGCC 
CTGGAACAGC ATCTTCCTGA GGATGACAAG ACCCCGATGT CCCGCAGACT GGAGAAGACA 
GAGCCGTATT TCATTGGGAT CTTTTGCTTT GAAGCCGGTA TCAAAATTGT GGCCCTGGGG 
TTCATCTTTC ATAAGGGCTC GTACCTCCGC AATGGCTGGA ATGTCATGGA CTTCATCGTG 
GTCCTCAGTG GCATCCTGGC CACTGCGGGG ACTCACTTCA ACACACACGT GGACCTGAGG 
ACCCTCCGGG CTGTGCGTGT CCTGAGACCC CTGAAGCTCG TGTCAGGGAT TCCTAGCCTG 
CAGATCGTGT TGAAGTCTAT CATGAAGGCC ATGGTGCCTC TTCTGCAAAT TGGTCTCCTG 
CTCTTCTTTG CCATCTTGAT GTTTGCTATC ATTGGCTTAG AGTTCTACAG TGGGAAGTTG 
CACCGAGCAT GTTTCACGAA CAATTCAGGT ATTCTGGAAG GATTTGACCC CCCTCACCCA 
TGTGGTGTGC AGGGCTGCCC AGCTGGCTAC GAATGCCGGG ACTGGATTGG CCCCAATGAT 
GGGATCACCC AGTTTGATAA CATCCTTTTT GCTGTGCTGA CTGTCTTCCA ATGCATCACC 
ATGGAAGGAT GGACCACCGT GCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC 
TGGCTGTACT TCATCCCCCT CATCATCATT GGATCCTTCT TTGTTCTCAA CCTAGTTCTG 
GGAGTGCTTT CCGGGGAGTT TGCCAAAGAG AGAGAGAGAG TGGAGAACCG GAGAGCTTTC 
ATGAAACTGC GGCGGCAGCA GCAGATTGAG CGGGAGCTGA ACGGGTACCG CGCCTGGATA 
GACAAAGCAG AGGAAGTCAT GCTTGCTGAA GAAAATAAAA ATGCTGGGAC GTCAGCCTTA 
GAAGTGCTTC GAAGGGCAAC CATCAAAAGG AGCCGGACAG AGGCCATGAC TCGAGACTCC 
AGTGATGAAC ACTGTGTCGA TATCTCCTCT GTGGGCACAC CTCTCGCCCG AGCCAGCATC 
AAAAGTGCAA AGGTGGATGG GGCCTCCTAT TTCCGGCACA AAGAAAGGCT TCTGCGCATC 
TCTATCCGCC ACATGGTGAA ATCCCAGGTG TTTTACTGGA TTGTGTTGAG CCTTGTGGCT 
CTCAACACTG CCTGTGTGGC CATCGTCCAT CATAACCAGC CCCAGTGGCT CACCCATCTC 
CTCTACTATG CAGAATTTTT GTTTCTGGGA CTGTTCCTCT TGGAGATGTC CCTGAAGATG 
TATGGCATGG GACCTCGACT TTATTTTCAC TCTTCATTCA ACTGTTTTGA TTTTGGGGTC 
ACAGTGGGCA GCATCTTTGA AGTGGTCTGG GCAATCTTCA GACCTGGCAC ATCTTTTGGA 
ATCAGTGTCT TACGGGCTCT CCGGCTTCTA AGAATATTTA AAATAACCAA GTATTGGGCA 
TCTCTGCGAA ATTTGGTGGT CTCCTTGATG AGCTCTATGA AGTCTATCAT CAGTTTGCTC 
TTCCTCCTCT TCCTCTTCAT CGTTGTCTTT GCTCTCCTAG GAATGCAGCT GTTTGGAGGC 
AGGTTTAACT TTAATGATGG GACTCCTTCA GCAAACTTTG ATACCTTCCC TGCAGCCATC 
ATGACTGTAT TCCAGATCCT AACAGGTGAG GACTGGAATG AGGTGATGTA CAATGGGATC 
CGTTCCCAGG GTGGAGTTAG CTCAGGCATG TGGTCAGCTA TCTATTTCAT TGTGCTCACC 
TTGTTTGGAA ACTACACACT GCTGAATGTG TTCTTGGCCA TTGCTGTGGA CAATCTGGCC 
AATGCCCAAG AGCTGACCAA GGATGAACAA GAAGAAGAGG AGGCTTTTAA CCAGAAACAT 
GCATTGCAGA AGGCCAAGGA GGTCAGCCCG ATGTCTGCAC CCAACATGCC TTCTATCGAG 
AGGGAGCGGA GGCGCCGGCA CCACATGTCG GTGTGGGAGC AGCGCACCAG CCAGCTGAGA 
AGGCACATGC AGATGTCCAG CCAGGAGGCC CTCAATAAAG AGGAGGCCCC ACCGATGAAC 
CCCCTCAACC CCCTCAACCC GCTGAGCCCC CTCAACCCGC TCAACGCCCA CCCCAGCCTC 
TACCGGAGAC CTAGGCCTAT TGAGGGCCTG GGCCTGGGCC TGGAGAAGTG TGAGGAGGAG 
CGCATCATCA GCCGCGGGGG GTCCCTCAAG GGGGACGGAG GGGACCTGTC GAGTGCCCTG 
GACAACCAGA GGAGCCCTTT GTCCCTGGGC AAACGGGAGC CACCATGGCT GGCCAGGCCC 
TGTCATGGGA ACTGTGATCC GACCCAGCAG GAGGCTGGGG GAGGGGAGAC CGTGGTGACC 
TTCGAGGACC GGGCCAGGCA CAGGCAGAGC CAGCGGCGCA GCCGGCACCG TCGTGTCAGG 
ACGGAAGGCA AGGAGCTCTC CTCCACCTCC CGGAGCAGGT CTGTCAGCCA GGAGCGGAGT 
CTGGATGAGG GGGTGCTCAC CGACGGGGAG AAGGAGCGAG AGCCCAGGGG CGGCCATGCA 
GGCAAAGAAC CCACCATCCA AGAAGAAGAC AGAGGCCAAG ACTTAAGGAG GACGAACAGT 
CTGATGGTGC CCAGAGGCTC TGGGCTGGCA GGAGCCCTTG ATGAGGCCAA CACACCCCTA 
GTCCTGCCCC ACCACGATCT GGAAGTGGGG AAGGACACGG TGCTGACGGA GCAGGAGCCG 
GAGGGCAGCA GCGAGCAGGC CCTGCTGGGG GATGTGCAGT TGGACATTGG CAGGGTCATC 
AGCCAGAGTG AGCCTGACCT CTCCTGCGTC ACAGCCAACA CAGACAAGGC CACCACCGAC 
AGCACCAGCG TCACCGTCGC CATCCCTGAT GTGGGCCCCT TGATGGACTC AACTGTGATT 
CACATTAGCA ACAAAACTGA TGGAGAAGCC AGTCCCTTGA AGGAGGCTGA GATCAAAGAG 
GAGGAGGAGG AAATGGAGAA GAAGCAGCAG AAGAAGGAGA AGCGTGAGAC GGGCAAAGCC 
ATGGTGCCCC ACAGCTCTAT GTTCATCTTC AGCACCACCA ACCCGATTCG CAGGGCCTGC 
CACTACATCG TGAACCTGCG CTACTTTGAG ATGTGCATTC TCCTGGTGAT CGCCGCCAGC 
AGCATTGCCC TGGCAGCAGA GGACCCCGTC CTGACCAACT CGGAGCGCAA CAAAGTCCTG 
AGGTATTTTG ACTATGTTTT CACTGGCGTG TTCACCTTTG AGATGGTTAT AAAGATGATA 
GACCAGGGCT TGATCCTACA GGATGGGTCC TACTTCCGAG ACCTGTGGAA CATCCTGGAC 
TTTGTGGTGG TGGTTGGTGC ATTGGTGGCC TTTGCTTTGG CGAACGCTTT GGGAACAAAC 
AAGGGGCGAG ACATCAAGAC CATCAAATCC CTGAGGGTGC TCCGAGTTCT GAGGCCACTG 
AAGACCATCA AGCGCTTGCC CAAACTCAAG GCTGTCTTTG ACTGTGTGGT GACCTCCTTG 
AAGAATGTCT TCAACATACT CATCGTCTAT AAGCTCTTCA TGTTCATCTT TGCTGTCATT 
GCGGTTCAGC TCTTCAAGGG AAAATTCTTT TATTGCACAG ACAGTTCCAA GGACACAGAG 
AAGGAGTGCA TAGGCAACTA CGTAGATCAT GAAAAAAACA AGATGGAGGT GAAAGGCCGG 
GAATGGAAGC GCCATGAATT CCACTACGAC AACATTATCT GGGCCCTGCT GACCCTCTTC 
ACCGTCTCCA CGGGGGAAGG ATGGCCTCAA GTCTTACAGC ATTCTGTCGA TGTGACAGAG 
GAAGACCGAG GACCAAGCCG CAGCAACCGC ATGGAGATGT CCATCTTCTA TGTGGTCTAC 
TTTGTGGTCT TCCCTTTCTT CTTTGTCAAT ATCTTTGTGG CTCTGATCAT CATCACCTTC 
CAGGAACAAG GGGACAAGAT GATGGAGGAA TGCAGCCTGG AGAAGAACGA GAGGGCGTGC 
ATTGACTTCG CCATCAGTGC CAAACCTCTC ACCCGCTACA TGCCACAGAA CAGGCACACC 
TTCCAGTACC GCGTCTGGCA CTTCGTGGTG TCTCCGTCCT TTGAGTACAC CATCATGGCC 
ATGATCGCCC TGAACACCGT CGTGCTGATG ATGAAGTACT ACTCTGCTCC CTGTACCTAT 
GAACTGGCCC TGAAGTACCT GAATATTGCC TTCACCATGG TGTTTTCCCT GGAATGTGTC 
CTAAAGATCA TCGCTTTTGG CTTCTTGAAC TACTTTCGAG ACACCTGGAA CATCTTTGAC 
TTCATCACTG TGATTGGCAG TATCACAGAA ATCATACTGA CAGACAGCAA GCTGGTAAAC 
ACCAGTGGTT TCAACATGAG CTTTCTGAAG CTCTTCCGTG CCGCCCGCCT CATCAAGCTT 
CTGCGGCAGG GCTACACCAT CCGTATTTTA CTCTGGACCT TCGTGCAGTC CTTTAAGGCC 
CTCCCCTACG TCTGCCTTTT GATTGCCATG CTTTTCTTCA TCTATGCCAT CATTGGGATG 
CAGGTATTTG GAAACATAAA ATTAGATGAG GAGAGTCACA TCAACCGGCA CAACAACTTC 
CGGAGTTTCT TTGGGTCCCT CATGTTACTC TTCAGGAGTG CCACGGGAGA GGCCTGGCAG 
GAGATAATGC TGTCGTGCCT CGGGGAGAAA GGCTGCGAGC CCGACACCAC TGCGCCATCG 
GGGCAGAACG AGAATGAGCG CTGTGGCACC GATCTGGCCT ATGTTTACTT TGTCTCCTTC 
ATCTTCTTCT GCTCTTTCTT GATGCTCAAC TTGTTTGTGG CCGTCATCAT GGACAACTTC 
GAGTACCTGA CTCGGGACTC CTCCATCTTA GGGCCCCACC ACTTGGATGA GTTTGTCCGC 
GTCTGGGCAG AGTACGACCG AGCTGCATGC GGCAGGATCC CGTATAAGGA TATGTACAAA 
TTAGTGCGGG TGATCTCGCC GCCTCTGGGC CTGGGAGAGA ACTGCCCCTA CAGGGTGGCT 
TGCAAGAGGT TGGTCCTAAT GAATATGCCA GTAGCTGAGG ACATGACAGT CCACTTCACC 
TCCACACTGA TGGCTCTGAT CCGGACAGCC CTGGACATCA AAATCGCCAA AGGTGGTGCA 
GACAGGCAGC AGCTAGACTC GGAGCTGCAA AAGGAGACCC TGGCCATCTG GCCTCACCTG 
TCCCAGAAGA TGCTAGACCT GCTTGTGCCC ATGCCCAAAG CCTCTGACCT GACTGTGGGC 
AAAATCTATG CAGCAATGAT GATCATGGAC TACTATAAAC AGAGTAAGGT GAAGAAACAG 
AGGCAGCAGC TGGAGGAACA GAAAAATGCA CCCATGTTCC AGCGAATGGA GCCCTCATCT 
CTGCCTCAGG AGATCATTGC TAATGCCAAA GCCCTGCCCT ATCTCCAGCA GGACCCCGTT 
TCAGGCCTGA GTGGCCGTAG TGGATACCCT TCGATGAGTC CACTCTCCCC CCAGGAAATA 
TTCCAATTGG CTTGTATGGA TCCAGCCGAT GATGGACAGT TCCAGGAACA TCAGTCTCTG 
GTGGTGACAG ATACTAGCTC CATGAGACGT TCATTTTCCA CTATTCGGGA TAAGCGTTCA 
AATTCCTCCT GGTTGGAGGA ATTCTCCATG GAGAGAAGCA GTGAAAACAC CTACAAGTCA 
CGTCGTCGGA GTTACCACTC CTCCCTGAGA CTGTCAGCTC ATCGCCTGAA TTCTGACTCA 
GGCCACAAGT CTGACACCCA CCGCTCAGGG GGTAGAGAGC GGGGACGATC GAAAGAGCGA 
AAGCATCTTC TCTCTCCTGA TGTCTCCCGC TGCAACTCAG AGGAGCGAGG GACCCAGGCC 
GACTGGGAGT CCCCAGAGCG CCAACAGTCC AGGTCTCCCA GTGAGGCCAG GTCACAGACC 
CCCAACAGAC AGGGCACGGG CTCCCTGAGC GAGAGCTCCA TCCCCTCCAT CTCTGACACC 
AGCACCCCAA GACGAAGTCG CCGGCAGCTC CCACCCGTCC CACCAAAGCC TCGGCCCCTC 
CTCTCCTACA GCTCCCTGAT TCGACACTCC GGGAGCATCT CCCCGCCGGC CGACGGAAGC 
GAGAGGGGCT CCCCGCTGAC CCCCCAGGCG CTGGAGAGCA CGGAGGCCGG CCTGGCGGAG 
CCCTCGGGCC CCCGGCACGG GCACGGGCAG GGGCACGGGC AGGGGCACGG GCAGCAGAGC 
CAGCACTGCT CCCCGCAGCG CTACATCTCC GAGCCCTACC TGGCCCTGCA CGAAGACTCC 
CACGCGTCGG ACTGTGGCGA GGAGGAGACG CTCACCTTCG AGGCCGCCGT GGCCACCAGC 
CTGGGCCGCT CCAACACCAT CGGCTCGGCC CCGCCGCTGC GGCACAGCTG GCAGATGCCC 
AACGGGCACT ATCGGCGGCG GCGGCGCGGG GGGCCGGGGC CGGGCGTGAT GTGCGGGGCG 
GTCAGCGACC TCCTGAGTGA CACGGAAGAA GACGACAAGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_014115117.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970592.1 Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OCe37413
Clone ID Related Accession (Same CDS sequence)	XM_014115117.1
Accession Version	XM_014115117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGC AATGTTCTGG GATGGTGCCC GAGATGCGTG GACCGAGCCC AGGCCGCAGG 
CTGCCCGAGC CCGCCCCCTG CCCCGCCGCG GCCGGGAGCC CTGAGCCCTG GGCCGCGGGC 
CGCCGCCGCC ACGTCCTGGC CCTGCCTGAG GAGGAGGCAT GGAGGCCCCA GCAGTGCTCC 
CTGCTCGAGG CCGACGCCTC GGACGAGGTG GGCATGCTGC CGGCCTCCCC GCGCGCCGCC 
GCCTCCGGCG TCGACAACTT CTTCCCGGTG CAGGAGGGCG AGGGCGCGGG CTGGGAGGGC 
GGTGCGGCCC TGGACGCCGG CGCCGGCCCC TTCCTCCCGG TGAGCCCCGA GGTGATGAAG 
CGGCGGCGCG GGGGCCTCAT CGAGCAGCGC GACATCATCA AGGCCCACGA GGCGCACAAG 
ATGCAGAGCA CCCCGCAGGC GCGGCGCAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG 
GTGGTCGGCA GGCCGGGGTC GGGCGATGGA GACTCGGACC AGAGCAGGAA CCGGCAAGGA 
ACCCCGGTGC CGGCCTCGGG GCCGGCGGCC GCCTACAAGC AGTCGAAAGC GCAGAGGGCG 
CGGACTATGG CTTTGTACAA CCCCATTCCC GTCCGGCAGA ACTGTTTCAC CGTCAACAGA 
TCCCTGTTCA TCTTCGGAGA AGATAACATT GTCAGGAAAT ACGCCAAGAA GCTCATCGAC 
TGGCCGCCGT TTGAGTACAT GATTTTGGCA ACCATCATTG CCAACTGCAT TGTCTTGGCC 
CTGGAACAGC ATCTTCCTGA GGATGACAAG ACCCCGATGT CCCGCAGACT GGAGAAGACA 
GAGCCGTATT TCATTGGGAT CTTTTGCTTT GAAGCCGGTA TCAAAATTGT GGCCCTGGGG 
TTCATCTTTC ATAAGGGCTC GTACCTCCGC AATGGCTGGA ATGTCATGGA CTTCATCGTG 
GTCCTCAGTG GCATCCTGGC CACTGCGGGG ACTCACTTCA ACACACACGT GGACCTGAGG 
ACCCTCCGGG CTGTGCGTGT CCTGAGACCC CTGAAGCTCG TGTCAGGGAT TCCTAGCCTG 
CAGATCGTGT TGAAGTCTAT CATGAAGGCC ATGGTGCCTC TTCTGCAAAT TGGTCTCCTG 
CTCTTCTTTG CCATCTTGAT GTTTGCTATC ATTGGCTTAG AGTTCTACAG TGGGAAGTTG 
CACCGAGCAT GTTTCACGAA CAATTCAGGT ATTCTGGAAG GATTTGACCC CCCTCACCCA 
TGTGGTGTGC AGGGCTGCCC AGCTGGCTAC GAATGCCGGG ACTGGATTGG CCCCAATGAT 
GGGATCACCC AGTTTGATAA CATCCTTTTT GCTGTGCTGA CTGTCTTCCA ATGCATCACC 
ATGGAAGGAT GGACCACCGT GCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC 
TGGCTGTACT TCATCCCCCT CATCATCATT GGATCCTTCT TTGTTCTCAA CCTAGTTCTG 
GGAGTGCTTT CCGGGGAGTT TGCCAAAGAG AGAGAGAGAG TGGAGAACCG GAGAGCTTTC 
ATGAAACTGC GGCGGCAGCA GCAGATTGAG CGGGAGCTGA ACGGGTACCG CGCCTGGATA 
GACAAAGCAG AGGAAGTCAT GCTTGCTGAA GAAAATAAAA ATGCTGGGAC GTCAGCCTTA 
GAAGTGCTTC GAAGGGCAAC CATCAAAAGG AGCCGGACAG AGGCCATGAC TCGAGACTCC 
AGTGATGAAC ACTGTGTCGA TATCTCCTCT GTGGGCACAC CTCTCGCCCG AGCCAGCATC 
AAAAGTGCAA AGGTGGATGG GGCCTCCTAT TTCCGGCACA AAGAAAGGCT TCTGCGCATC 
TCTATCCGCC ACATGGTGAA ATCCCAGGTG TTTTACTGGA TTGTGTTGAG CCTTGTGGCT 
CTCAACACTG CCTGTGTGGC CATCGTCCAT CATAACCAGC CCCAGTGGCT CACCCATCTC 
CTCTACTATG CAGAATTTTT GTTTCTGGGA CTGTTCCTCT TGGAGATGTC CCTGAAGATG 
TATGGCATGG GACCTCGACT TTATTTTCAC TCTTCATTCA ACTGTTTTGA TTTTGGGGTC 
ACAGTGGGCA GCATCTTTGA AGTGGTCTGG GCAATCTTCA GACCTGGCAC ATCTTTTGGA 
ATCAGTGTCT TACGGGCTCT CCGGCTTCTA AGAATATTTA AAATAACCAA GTATTGGGCA 
TCTCTGCGAA ATTTGGTGGT CTCCTTGATG AGCTCTATGA AGTCTATCAT CAGTTTGCTC 
TTCCTCCTCT TCCTCTTCAT CGTTGTCTTT GCTCTCCTAG GAATGCAGCT GTTTGGAGGC 
AGGTTTAACT TTAATGATGG GACTCCTTCA GCAAACTTTG ATACCTTCCC TGCAGCCATC 
ATGACTGTAT TCCAGATCCT AACAGGTGAG GACTGGAATG AGGTGATGTA CAATGGGATC 
CGTTCCCAGG GTGGAGTTAG CTCAGGCATG TGGTCAGCTA TCTATTTCAT TGTGCTCACC 
TTGTTTGGAA ACTACACACT GCTGAATGTG TTCTTGGCCA TTGCTGTGGA CAATCTGGCC 
AATGCCCAAG AGCTGACCAA GGATGAACAA GAAGAAGAGG AGGCTTTTAA CCAGAAACAT 
GCATTGCAGA AGGCCAAGGA GGTCAGCCCG ATGTCTGCAC CCAACATGCC TTCTATCGAA 
AGAGACAGAA GGAGAAGACA CCACATGTCG ATGTGGGAGC CACGCAGCAG CCACCTGAGG 
GAGCGGAGGC GCCGGCACCA CATGTCGGTG TGGGAGCAGC GCACCAGCCA GCTGAGAAGG 
CACATGCAGA TGTCCAGCCA GGAGGCCCTC AATAAAGAGG AGGCCCCACC GATGAACCCC 
CTCAACCCCC TCAACCCGCT GAGCCCCCTC AACCCGCTCA ACGCCCACCC CAGCCTCTAC 
CGGAGACCTA GGCCTATTGA GGGCCTGGGC CTGGGCCTGG AGAAGTGTGA GGAGGAGCGC 
ATCATCAGCC GCGGGGGGTC CCTCAAGGGG GACGGAGGGG ACCTGTCGAG TGCCCTGGAC 
AACCAGAGGA GCCCTTTGTC CCTGGGCAAA CGGGAGCCAC CATGGCTGGC CAGGCCCTGT 
CATGGGAACT GTGATCCGAC CCAGCAGGAG GCTGGGGGAG GGGAGACCGT GGTGACCTTC 
GAGGACCGGG CCAGGCACAG GCAGAGCCAG CGGCGCAGCC GGCACCGTCG TGTCAGGACG 
GAAGGCAAGG AGCTCTCCTC CACCTCCCGG AGCAGGTCTG TCAGCCAGGA GCGGAGTCTG 
GATGAGGGGG TGCTCACCGA CGGGGAGAAG GAGCGAGAGC CCAGGGGCGG CCATGCAGGC 
AAAGAACCCA CCATCCAAGA AGAAGACAGA GGCCAAGACT TAAGGAGGAC GAACAGTCTG 
ATGGTGCCCA GAGGCTCTGG GCTGGCAGGA GCCCTTGATG AGGCCAACAC ACCCCTAGTC 
CTGCCCCACC ACGATCTGGA AGTGGGGAAG GACACGGTGC TGACGGAGCA GGAGCCGGAG 
GGCAGCAGCG AGCAGGCCCT GCTGGGGGAT GTGCAGTTGG ACATTGGCAG GGTCATCAGC 
CAGAGTGAGC CTGACCTCTC CTGCGTCACA GCCAACACAG ACAAGGCCAC CACCGACAGC 
ACCAGCGTCA CCGTCGCCAT CCCTGATGTG GGCCCCTTGA TGGACTCAAC TGTGATTCAC 
ATTAGCAACA AAACTGATGG AGAAGCCAGT CCCTTGAAGG AGGCTGAGAT CAAAGAGGAG 
GAGGAGGAAA TGGAGAAGAA GCAGCAGAAG AAGGAGAAGC GTGAGACGGG CAAAGCCATG 
GTGCCCCACA GCTCTATGTT CATCTTCAGC ACCACCAACC CGATTCGCAG GGCCTGCCAC 
TACATCGTGA ACCTGCGCTA CTTTGAGATG TGCATTCTCC TGGTGATCGC CGCCAGCAGC 
ATTGCCCTGG CAGCAGAGGA CCCCGTCCTG ACCAACTCGG AGCGCAACAA AGTCCTGAGG 
TATTTTGACT ATGTTTTCAC TGGCGTGTTC ACCTTTGAGA TGGTTATAAA GATGATAGAC 
CAGGGCTTGA TCCTACAGGA TGGGTCCTAC TTCCGAGACC TGTGGAACAT CCTGGACTTT 
GTGGTGGTGG TTGGTGCATT GGTGGCCTTT GCTTTGGCAA CAAACAAGGG GCGAGACATC 
AAGACCATCA AATCCCTGAG GGTGCTCCGA GTTCTGAGGC CACTGAAGAC CATCAAGCGC 
TTGCCCAAAC TCAAGGCTGT CTTTGACTGT GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATCG TCTATAAGCT CTTCATGTTC ATCTTTGCTG TCATTGCGGT TCAGCTCTTC 
AAGGGAAAAT TCTTTTATTG CACAGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTACGTAG ATCATGAAAA AAACAAGATG GAGGTGAAAG GCCGGGAATG GAAGCGCCAT 
GAATTCCACT ACGACAACAT TATCTGGGCC CTGCTGACCC TCTTCACCGT CTCCACGGGG 
GAAGGATGGC CTCAAGTCTT ACAGCATTCT GTCGATGTGA CAGAGGAAGA CCGAGGACCA 
AGCCGCAGCA ACCGCATGGA GATGTCCATC TTCTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT TGTGGCTCTG ATCATCATCA CCTTCCAGGA ACAAGGGGAC 
AAGATGATGG AGGAATGCAG CCTGGAGAAG AACGAGAGGG CGTGCATTGA CTTCGCCATC 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGCGTC 
TGGCACTTCG TGGTGTCTCC GTCCTTTGAG TACACCATCA TGGCCATGAT CGCCCTGAAC 
ACCGTCGTGC TGATGATGAA GTACTACTCT GCTCCCTGTA CCTATGAACT GGCCCTGAAG 
TACCTGAATA TTGCCTTCAC CATGGTGTTT TCCCTGGAAT GTGTCCTAAA GATCATCGCT 
TTTGGCTTCT TGAACTACTT TCGAGACACC TGGAACATCT TTGACTTCAT CACTGTGATT 
GGCAGTATCA CAGAAATCAT ACTGACAGAC AGCAAGCTGG TAAACACCAG TGGTTTCAAC 
ATGAGCTTTC TGAAGCTCTT CCGTGCCGCC CGCCTCATCA AGCTTCTGCG GCAGGGCTAC 
ACCATCCGTA TTTTACTCTG GACCTTCGTG CAGTCCTTTA AGGCCCTCCC CTACGTCTGC 
CTTTTGATTG CCATGCTTTT CTTCATCTAT GCCATCATTG GGATGCAGGT ATTTGGAAAC 
ATAAAATTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGG 
TCCCTCATGT TACTCTTCAG GAGTGCCACG GGAGAGGCCT GGCAGGAGAT AATGCTGTCG 
TGCCTCGGGG AGAAAGGCTG CGAGCCCGAC ACCACTGCGC CATCGGGGCA GAACGAGAAT 
GAGCGCTGTG GCACCGATCT GGCCTATGTT TACTTTGTCT CCTTCATCTT CTTCTGCTCT 
TTCTTGATGC TCAACTTGTT TGTGGCCGTC ATCATGGACA ACTTCGAGTA CCTGACTCGG 
GACTCCTCCA TCTTAGGGCC CCACCACTTG GATGAGTTTG TCCGCGTCTG GGCAGAGTAC 
GACCGAGCTG CATGCGGCAG GATCCCGTAT AAGGATATGT ACAAATTAGT GCGGGTGATC 
TCGCCGCCTC TGGGCCTGGG AGAGAACTGC CCCTACAGGG TGGCTTGCAA GAGGTTGGTC 
CTAATGAATA TGCCAGTAGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTGATGGCT 
CTGATCCGGA CAGCCCTGGA CATCAAAATC GCCAAAGGTG GTGCAGACAG GCAGCAGCTA 
GACTCGGAGC TGCAAAAGGA GACCCTGGCC ATCTGGCCTC ACCTGTCCCA GAAGATGCTA 
GACCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAAAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTGAAGA AACAGAGGCA GCAGCTGGAG 
GAACAGAAAA ATGCACCCAT GTTCCAGCGA ATGGAGCCCT CATCTCTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCCTATCTC CAGCAGGACC CCGTTTCAGG CCTGAGTGGC 
CGTAGTGGAT ACCCTTCGAT GAGTCCACTC TCCCCCCAGG AAATATTCCA ATTGGCTTGT 
ATGGATCCAG CCGATGATGG ACAGTTCCAG GAACATCAGT CTCTGGAGCC AGAGGTTAGT 
GAATTAAAAA GCGTGCAGTC CTCTAACCAT GGCATCTACC TTCCTTCGGA CACCCAGGAG 
CATACGGGAT CTGGGAGGGC ATCTTCTATG CCACGCCTGA CTGTGGATCC CCAGGTGGTG 
ACAGATACTA GCTCCATGAG ACGTTCATTT TCCACTATTC GGGATAAGCG TTCAAATTCC 
TCCTGGTTGG AGGAATTCTC CATGGAGAGA AGCAGTGAAA ACACCTACAA GTCACGTCGT 
CGGAGTTACC ACTCCTCCCT GAGACTGTCA GCTCATCGCC TGAATTCTGA CTCAGGCCAC 
AAGTCTGACA CCCACCGCTC AGGGGGTAGA GAGCGGGGAC GATCGAAAGA GCGAAAGCAT 
CTTCTCTCTC CTGATGTCTC CCGCTGCAAC TCAGAGGAGC GAGGGACCCA GGCCGACTGG 
GAGTCCCCAG AGCGCCAACA GTCCAGGTCT CCCAGTGAGG CCAGGTCACA GACCCCCAAC 
AGACAGGGCA CGGGCTCCCT GAGCGAGAGC TCCATCCCCT CCATCTCTGA CACCAGCACC 
CCAAGACGAA GTCGCCGGCA GCTCCCACCC GTCCCACCAA AGCCTCGGCC CCTCCTCTCC 
TACAGCTCCC TGATTCGACA CTCCGGGAGC ATCTCCCCGC CGGCCGACGG AAGCGAGAGG 
GGCTCCCCGC TGACCCCCCA GGCGCTGGAG AGCACGGAGG CCGGCCTGGC GGAGCCCTCG 
GGCCCCCGGC ACGGGCACGG GCAGGGGCAC GGGCAGGGGC ACGGGCAGCA GAGCCAGCAC 
TGCTCCCCGC AGCGCTACAT CTCCGAGCCC TACCTGGCCC TGCACGAAGA CTCCCACGCG 
TCGGACTGTG GCGAGGAGGA GACGCTCACC TTCGAGGCCG CCGTGGCCAC CAGCCTGGGC 
CGCTCCAACA CCATCGGCTC GGCCCCGCCG CTGCGGCACA GCTGGCAGAT GCCCAACGGG 
CACTATCGGC GGCGGCGGCG CGGGGGGCCG GGGCCGGGCG TGATGTGCGG GGCGGTCAGC 
GACCTCCTGA GTGACACGGA AGAAGACGAC AAGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970592.1
CDS	86..7504
Translation	MEEQCSGMVPEMRGPSPGRRLPEPAPCPAAAGSPEPWAAGRRRHVLALPEEEAWRPQQCSLLEADASDEVGMLPASPRAAASGVDNFFPVQEGEGAGWEGGAALDAGAGPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFTNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLGLGLEKCEEERIISRGGSLKGDGGDLSSALDNQRSPLSLGKREPPWLARPCHGNCDPTQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKELSSTSRSRSVSQERSLDEGVLTDGEKEREPRGGHAGKEPTIQEEDRGQDLRRTNSLMVPRGSGLAGALDEANTPLVLPHHDLEVGKDTVLTEQEPEGSSEQALLGDVQLDIGRVISQSEPDLSCVTANTDKATTDSTSVTVAIPDVGPLMDSTVIHISNKTDGEASPLKEAEIKEEEEEMEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALATNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIPYKDMYKLVRVISPPLGLGENCPYRVACKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEHQSLEPEVSELKSVQSSNHGIYLPSDTQEHTGSGRASSMPRLTVDPQVVTDTSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERQQSRSPSEARSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHSGSISPPADGSERGSPLTPQALESTEAGLAEPSGPRHGHGQGHGQGHGQQSQHCSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGVMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_014115117.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115117.1

ORF Insert Sequence:

ATGGAAGAGC AATGTTCTGG GATGGTGCCC GAGATGCGTG GACCGAGCCC AGGCCGCAGG 
CTGCCCGAGC CCGCCCCCTG CCCCGCCGCG GCCGGGAGCC CTGAGCCCTG GGCCGCGGGC 
CGCCGCCGCC ACGTCCTGGC CCTGCCTGAG GAGGAGGCAT GGAGGCCCCA GCAGTGCTCC 
CTGCTCGAGG CCGACGCCTC GGACGAGGTG GGCATGCTGC CGGCCTCCCC GCGCGCCGCC 
GCCTCCGGCG TCGACAACTT CTTCCCGGTG CAGGAGGGCG AGGGCGCGGG CTGGGAGGGC 
GGTGCGGCCC TGGACGCCGG CGCCGGCCCC TTCCTCCCGG TGAGCCCCGA GGTGATGAAG 
CGGCGGCGCG GGGGCCTCAT CGAGCAGCGC GACATCATCA AGGCCCACGA GGCGCACAAG 
ATGCAGAGCA CCCCGCAGGC GCGGCGCAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG 
GTGGTCGGCA GGCCGGGGTC GGGCGATGGA GACTCGGACC AGAGCAGGAA CCGGCAAGGA 
ACCCCGGTGC CGGCCTCGGG GCCGGCGGCC GCCTACAAGC AGTCGAAAGC GCAGAGGGCG 
CGGACTATGG CTTTGTACAA CCCCATTCCC GTCCGGCAGA ACTGTTTCAC CGTCAACAGA 
TCCCTGTTCA TCTTCGGAGA AGATAACATT GTCAGGAAAT ACGCCAAGAA GCTCATCGAC 
TGGCCGCCGT TTGAGTACAT GATTTTGGCA ACCATCATTG CCAACTGCAT TGTCTTGGCC 
CTGGAACAGC ATCTTCCTGA GGATGACAAG ACCCCGATGT CCCGCAGACT GGAGAAGACA 
GAGCCGTATT TCATTGGGAT CTTTTGCTTT GAAGCCGGTA TCAAAATTGT GGCCCTGGGG 
TTCATCTTTC ATAAGGGCTC GTACCTCCGC AATGGCTGGA ATGTCATGGA CTTCATCGTG 
GTCCTCAGTG GCATCCTGGC CACTGCGGGG ACTCACTTCA ACACACACGT GGACCTGAGG 
ACCCTCCGGG CTGTGCGTGT CCTGAGACCC CTGAAGCTCG TGTCAGGGAT TCCTAGCCTG 
CAGATCGTGT TGAAGTCTAT CATGAAGGCC ATGGTGCCTC TTCTGCAAAT TGGTCTCCTG 
CTCTTCTTTG CCATCTTGAT GTTTGCTATC ATTGGCTTAG AGTTCTACAG TGGGAAGTTG 
CACCGAGCAT GTTTCACGAA CAATTCAGGT ATTCTGGAAG GATTTGACCC CCCTCACCCA 
TGTGGTGTGC AGGGCTGCCC AGCTGGCTAC GAATGCCGGG ACTGGATTGG CCCCAATGAT 
GGGATCACCC AGTTTGATAA CATCCTTTTT GCTGTGCTGA CTGTCTTCCA ATGCATCACC 
ATGGAAGGAT GGACCACCGT GCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC 
TGGCTGTACT TCATCCCCCT CATCATCATT GGATCCTTCT TTGTTCTCAA CCTAGTTCTG 
GGAGTGCTTT CCGGGGAGTT TGCCAAAGAG AGAGAGAGAG TGGAGAACCG GAGAGCTTTC 
ATGAAACTGC GGCGGCAGCA GCAGATTGAG CGGGAGCTGA ACGGGTACCG CGCCTGGATA 
GACAAAGCAG AGGAAGTCAT GCTTGCTGAA GAAAATAAAA ATGCTGGGAC GTCAGCCTTA 
GAAGTGCTTC GAAGGGCAAC CATCAAAAGG AGCCGGACAG AGGCCATGAC TCGAGACTCC 
AGTGATGAAC ACTGTGTCGA TATCTCCTCT GTGGGCACAC CTCTCGCCCG AGCCAGCATC 
AAAAGTGCAA AGGTGGATGG GGCCTCCTAT TTCCGGCACA AAGAAAGGCT TCTGCGCATC 
TCTATCCGCC ACATGGTGAA ATCCCAGGTG TTTTACTGGA TTGTGTTGAG CCTTGTGGCT 
CTCAACACTG CCTGTGTGGC CATCGTCCAT CATAACCAGC CCCAGTGGCT CACCCATCTC 
CTCTACTATG CAGAATTTTT GTTTCTGGGA CTGTTCCTCT TGGAGATGTC CCTGAAGATG 
TATGGCATGG GACCTCGACT TTATTTTCAC TCTTCATTCA ACTGTTTTGA TTTTGGGGTC 
ACAGTGGGCA GCATCTTTGA AGTGGTCTGG GCAATCTTCA GACCTGGCAC ATCTTTTGGA 
ATCAGTGTCT TACGGGCTCT CCGGCTTCTA AGAATATTTA AAATAACCAA GTATTGGGCA 
TCTCTGCGAA ATTTGGTGGT CTCCTTGATG AGCTCTATGA AGTCTATCAT CAGTTTGCTC 
TTCCTCCTCT TCCTCTTCAT CGTTGTCTTT GCTCTCCTAG GAATGCAGCT GTTTGGAGGC 
AGGTTTAACT TTAATGATGG GACTCCTTCA GCAAACTTTG ATACCTTCCC TGCAGCCATC 
ATGACTGTAT TCCAGATCCT AACAGGTGAG GACTGGAATG AGGTGATGTA CAATGGGATC 
CGTTCCCAGG GTGGAGTTAG CTCAGGCATG TGGTCAGCTA TCTATTTCAT TGTGCTCACC 
TTGTTTGGAA ACTACACACT GCTGAATGTG TTCTTGGCCA TTGCTGTGGA CAATCTGGCC 
AATGCCCAAG AGCTGACCAA GGATGAACAA GAAGAAGAGG AGGCTTTTAA CCAGAAACAT 
GCATTGCAGA AGGCCAAGGA GGTCAGCCCG ATGTCTGCAC CCAACATGCC TTCTATCGAA 
AGAGACAGAA GGAGAAGACA CCACATGTCG ATGTGGGAGC CACGCAGCAG CCACCTGAGG 
GAGCGGAGGC GCCGGCACCA CATGTCGGTG TGGGAGCAGC GCACCAGCCA GCTGAGAAGG 
CACATGCAGA TGTCCAGCCA GGAGGCCCTC AATAAAGAGG AGGCCCCACC GATGAACCCC 
CTCAACCCCC TCAACCCGCT GAGCCCCCTC AACCCGCTCA ACGCCCACCC CAGCCTCTAC 
CGGAGACCTA GGCCTATTGA GGGCCTGGGC CTGGGCCTGG AGAAGTGTGA GGAGGAGCGC 
ATCATCAGCC GCGGGGGGTC CCTCAAGGGG GACGGAGGGG ACCTGTCGAG TGCCCTGGAC 
AACCAGAGGA GCCCTTTGTC CCTGGGCAAA CGGGAGCCAC CATGGCTGGC CAGGCCCTGT 
CATGGGAACT GTGATCCGAC CCAGCAGGAG GCTGGGGGAG GGGAGACCGT GGTGACCTTC 
GAGGACCGGG CCAGGCACAG GCAGAGCCAG CGGCGCAGCC GGCACCGTCG TGTCAGGACG 
GAAGGCAAGG AGCTCTCCTC CACCTCCCGG AGCAGGTCTG TCAGCCAGGA GCGGAGTCTG 
GATGAGGGGG TGCTCACCGA CGGGGAGAAG GAGCGAGAGC CCAGGGGCGG CCATGCAGGC 
AAAGAACCCA CCATCCAAGA AGAAGACAGA GGCCAAGACT TAAGGAGGAC GAACAGTCTG 
ATGGTGCCCA GAGGCTCTGG GCTGGCAGGA GCCCTTGATG AGGCCAACAC ACCCCTAGTC 
CTGCCCCACC ACGATCTGGA AGTGGGGAAG GACACGGTGC TGACGGAGCA GGAGCCGGAG 
GGCAGCAGCG AGCAGGCCCT GCTGGGGGAT GTGCAGTTGG ACATTGGCAG GGTCATCAGC 
CAGAGTGAGC CTGACCTCTC CTGCGTCACA GCCAACACAG ACAAGGCCAC CACCGACAGC 
ACCAGCGTCA CCGTCGCCAT CCCTGATGTG GGCCCCTTGA TGGACTCAAC TGTGATTCAC 
ATTAGCAACA AAACTGATGG AGAAGCCAGT CCCTTGAAGG AGGCTGAGAT CAAAGAGGAG 
GAGGAGGAAA TGGAGAAGAA GCAGCAGAAG AAGGAGAAGC GTGAGACGGG CAAAGCCATG 
GTGCCCCACA GCTCTATGTT CATCTTCAGC ACCACCAACC CGATTCGCAG GGCCTGCCAC 
TACATCGTGA ACCTGCGCTA CTTTGAGATG TGCATTCTCC TGGTGATCGC CGCCAGCAGC 
ATTGCCCTGG CAGCAGAGGA CCCCGTCCTG ACCAACTCGG AGCGCAACAA AGTCCTGAGG 
TATTTTGACT ATGTTTTCAC TGGCGTGTTC ACCTTTGAGA TGGTTATAAA GATGATAGAC 
CAGGGCTTGA TCCTACAGGA TGGGTCCTAC TTCCGAGACC TGTGGAACAT CCTGGACTTT 
GTGGTGGTGG TTGGTGCATT GGTGGCCTTT GCTTTGGCAA CAAACAAGGG GCGAGACATC 
AAGACCATCA AATCCCTGAG GGTGCTCCGA GTTCTGAGGC CACTGAAGAC CATCAAGCGC 
TTGCCCAAAC TCAAGGCTGT CTTTGACTGT GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATCG TCTATAAGCT CTTCATGTTC ATCTTTGCTG TCATTGCGGT TCAGCTCTTC 
AAGGGAAAAT TCTTTTATTG CACAGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTACGTAG ATCATGAAAA AAACAAGATG GAGGTGAAAG GCCGGGAATG GAAGCGCCAT 
GAATTCCACT ACGACAACAT TATCTGGGCC CTGCTGACCC TCTTCACCGT CTCCACGGGG 
GAAGGATGGC CTCAAGTCTT ACAGCATTCT GTCGATGTGA CAGAGGAAGA CCGAGGACCA 
AGCCGCAGCA ACCGCATGGA GATGTCCATC TTCTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT TGTGGCTCTG ATCATCATCA CCTTCCAGGA ACAAGGGGAC 
AAGATGATGG AGGAATGCAG CCTGGAGAAG AACGAGAGGG CGTGCATTGA CTTCGCCATC 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGCGTC 
TGGCACTTCG TGGTGTCTCC GTCCTTTGAG TACACCATCA TGGCCATGAT CGCCCTGAAC 
ACCGTCGTGC TGATGATGAA GTACTACTCT GCTCCCTGTA CCTATGAACT GGCCCTGAAG 
TACCTGAATA TTGCCTTCAC CATGGTGTTT TCCCTGGAAT GTGTCCTAAA GATCATCGCT 
TTTGGCTTCT TGAACTACTT TCGAGACACC TGGAACATCT TTGACTTCAT CACTGTGATT 
GGCAGTATCA CAGAAATCAT ACTGACAGAC AGCAAGCTGG TAAACACCAG TGGTTTCAAC 
ATGAGCTTTC TGAAGCTCTT CCGTGCCGCC CGCCTCATCA AGCTTCTGCG GCAGGGCTAC 
ACCATCCGTA TTTTACTCTG GACCTTCGTG CAGTCCTTTA AGGCCCTCCC CTACGTCTGC 
CTTTTGATTG CCATGCTTTT CTTCATCTAT GCCATCATTG GGATGCAGGT ATTTGGAAAC 
ATAAAATTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGG 
TCCCTCATGT TACTCTTCAG GAGTGCCACG GGAGAGGCCT GGCAGGAGAT AATGCTGTCG 
TGCCTCGGGG AGAAAGGCTG CGAGCCCGAC ACCACTGCGC CATCGGGGCA GAACGAGAAT 
GAGCGCTGTG GCACCGATCT GGCCTATGTT TACTTTGTCT CCTTCATCTT CTTCTGCTCT 
TTCTTGATGC TCAACTTGTT TGTGGCCGTC ATCATGGACA ACTTCGAGTA CCTGACTCGG 
GACTCCTCCA TCTTAGGGCC CCACCACTTG GATGAGTTTG TCCGCGTCTG GGCAGAGTAC 
GACCGAGCTG CATGCGGCAG GATCCCGTAT AAGGATATGT ACAAATTAGT GCGGGTGATC 
TCGCCGCCTC TGGGCCTGGG AGAGAACTGC CCCTACAGGG TGGCTTGCAA GAGGTTGGTC 
CTAATGAATA TGCCAGTAGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTGATGGCT 
CTGATCCGGA CAGCCCTGGA CATCAAAATC GCCAAAGGTG GTGCAGACAG GCAGCAGCTA 
GACTCGGAGC TGCAAAAGGA GACCCTGGCC ATCTGGCCTC ACCTGTCCCA GAAGATGCTA 
GACCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAAAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTGAAGA AACAGAGGCA GCAGCTGGAG 
GAACAGAAAA ATGCACCCAT GTTCCAGCGA ATGGAGCCCT CATCTCTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCCTATCTC CAGCAGGACC CCGTTTCAGG CCTGAGTGGC 
CGTAGTGGAT ACCCTTCGAT GAGTCCACTC TCCCCCCAGG AAATATTCCA ATTGGCTTGT 
ATGGATCCAG CCGATGATGG ACAGTTCCAG GAACATCAGT CTCTGGAGCC AGAGGTTAGT 
GAATTAAAAA GCGTGCAGTC CTCTAACCAT GGCATCTACC TTCCTTCGGA CACCCAGGAG 
CATACGGGAT CTGGGAGGGC ATCTTCTATG CCACGCCTGA CTGTGGATCC CCAGGTGGTG 
ACAGATACTA GCTCCATGAG ACGTTCATTT TCCACTATTC GGGATAAGCG TTCAAATTCC 
TCCTGGTTGG AGGAATTCTC CATGGAGAGA AGCAGTGAAA ACACCTACAA GTCACGTCGT 
CGGAGTTACC ACTCCTCCCT GAGACTGTCA GCTCATCGCC TGAATTCTGA CTCAGGCCAC 
AAGTCTGACA CCCACCGCTC AGGGGGTAGA GAGCGGGGAC GATCGAAAGA GCGAAAGCAT 
CTTCTCTCTC CTGATGTCTC CCGCTGCAAC TCAGAGGAGC GAGGGACCCA GGCCGACTGG 
GAGTCCCCAG AGCGCCAACA GTCCAGGTCT CCCAGTGAGG CCAGGTCACA GACCCCCAAC 
AGACAGGGCA CGGGCTCCCT GAGCGAGAGC TCCATCCCCT CCATCTCTGA CACCAGCACC 
CCAAGACGAA GTCGCCGGCA GCTCCCACCC GTCCCACCAA AGCCTCGGCC CCTCCTCTCC 
TACAGCTCCC TGATTCGACA CTCCGGGAGC ATCTCCCCGC CGGCCGACGG AAGCGAGAGG 
GGCTCCCCGC TGACCCCCCA GGCGCTGGAG AGCACGGAGG CCGGCCTGGC GGAGCCCTCG 
GGCCCCCGGC ACGGGCACGG GCAGGGGCAC GGGCAGGGGC ACGGGCAGCA GAGCCAGCAC 
TGCTCCCCGC AGCGCTACAT CTCCGAGCCC TACCTGGCCC TGCACGAAGA CTCCCACGCG 
TCGGACTGTG GCGAGGAGGA GACGCTCACC TTCGAGGCCG CCGTGGCCAC CAGCCTGGGC 
CGCTCCAACA CCATCGGCTC GGCCCCGCCG CTGCGGCACA GCTGGCAGAT GCCCAACGGG 
CACTATCGGC GGCGGCGGCG CGGGGGGCCG GGGCCGGGCG TGATGTGCGG GGCGGTCAGC 
GACCTCCTGA GTGACACGGA AGAAGACGAC AAGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CACNA1E
Entrez Gene ID	490305
Full Name	calcium voltage-gated channel subunit alpha1 E
General protein information	Preferred Names calcium voltage-gated channel subunit alpha1 E Names voltage-dependent R-type calcium channel subunit alpha-1E
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Rattus norvegicus (Norway rat)	Cacna1e	NP_062167.2
Xenopus tropicalis (tropical clawed frog)	cacna1e	XP_002937409.2
Homo sapiens (human)	CACNA1E	NP_001192222.1
Bos taurus (cattle)	CACNA1E	NP_001178052.1
Mus musculus (house mouse)	Cacna1e	NP_033912.2
Gallus gallus (chicken)	CACNA1E	XP_422255.4
Pan troglodytes (chimpanzee)	CACNA1E	XP_003949701.1
	CACNA1E	XP_001110352.2
Canis lupus familiaris (dog)	CACNA1E	XP_547425.4
Danio rerio (zebrafish)	LOC564274	XP_001920777.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

CACNA1E cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム