TOP3A cDNA ORF clone, Canis lupus familiaris(Dog)

The following TOP3A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP3A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe06425 XM_546656.6
Latest version!
Canis lupus familiaris DNA topoisomerase III alpha (TOP3A), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe06426 XM_005620144.2
Latest version!
Canis lupus familiaris topoisomerase (DNA) III alpha (TOP3A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe36494 XM_014113658.1
Latest version!
Canis lupus familiaris topoisomerase (DNA) III alpha (TOP3A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
View Old Accession Versions >>
OCe06425 XM_546656.5 Canis lupus familiaris topoisomerase (DNA) III alpha (TOP3A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe06425
    Clone ID Related Accession (Same CDS sequence) XM_546656.6 , XM_546656.5
    Accession Version XM_546656.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2970bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product DNA topoisomerase 3-alpha
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_546656.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATCTTCC CGGTCGCCCG CAGCGCGCTC CGCTGGCTGC TACGGCCGGG AGCCCGGACC 
    TTGTCCCGTG CTGCCATGGA GGTGGCCTTC CGCGGTGTGC GCAAAATTCT CTGTGTGGCC
    GAGAAAAACG ACGCGGCCAA GGGGATCGCC GACCTGCTGT CCAACGGCCG CATGAGGCGG
    AGGGAGGGAT TTTCAAAATT CAACAAGATC TATGAATTTG ATTATCATCT ATATGGCCAG
    AATGTTACCA TGGTAATGAC TTCAGTTTCT GGACATTTGC TGGCTCACGA TTTCCAGATG
    CAGTTTCGCA AATGGCAGAG CTGCAATCCC CTTGTCCTCT TTGAAGCAGA AATTGAAAAG
    TACTGCCCAG AGAATTTTGT AGGCATCAAG AAAACTCTGC AGCGAGAGAC TCAGCAATGC
    CAGGCCCTGG TGATCTGGAC CGACTGTGAC AGAGAAGGTG AAAATATCGG GTTTGAAATT
    ATCCACGTAT GCAGGGCTGT GAAGCCAAAC CTGCAGGTGC TGAGAGCCCG TTTCTCTGAG
    ATCACCCCCC GAGCTGTCCG GGTGGCCTGT GAAAACCTGA CTGAGCCCGA TCAGAGAGTG
    AGCGATGCCG TGGATGTGAG GCAGGAGTTG GACCTGAGGA TTGGAGCTGC CTTCACTAGG
    TTTCAAACCC TGAGGCTTCA GAAGATTTTT CCCGAGGTGT TAGCAGAACA GCTGATCAGT
    TATGGCAGCT GCCAGTTCCC AACCCTGGGG TTCGTGGTGG AGAGGTTCAA AGCCATTCAG
    GCTTTTGTGC CAGAAATCTT CCACAAAATT AAAGTAACTC ATGACCACAA AGATGGCATC
    GTAGAATTCA ACTGGAAAAG GCATCGTCTC TTTAACCACA CAGCTTGTCT TGTCCTCTAT
    CAGCTGTGCA TGGAGGATCC CACGGCAACT GTGGTGGAGG TCAGGTCTAA GTCAAAGAGC
    AAATGGAGGC CTCAAGCGTT GGACACTGTG GAGCTTGAGA AGCTGGCTTC TCGAAAGTTG
    AGAATAAATG CTAAAGAAAC CATGAGGATT GCCGAAAAGC TCTATACTCA AGGGTACATC
    AGCTATCCTC GAACTGAAAC AAACATTTTC CCCAAAGACT TAGACCTGAC AGTGCTGGTG
    GAGCAGCAGA CCCCGGATCC ACGCTGGGGG GCCTTTGCCC AGAGCATTCT AGAGCGGGGT
    GGCCCCACTC CACGCAATGG GAACAAGTCT GACCAGGCTC ACCCTCCCAT CCACCCCACC
    AAATACACCA GCAGCTTGCA GGGAGATGAG CAGCGGCTGT ATGAGTTCAT CGTGCGGCAT
    TTCCTGGCTT GCTGCTCCCA GGATGCCCAG GGGCAGGAGA CCACTGTGGA GATTGACATC
    GCTCAGGAAC GCTTCGTGGC CCACGGCCTC ATGATTTTGG CCCGAAACTA TTTGGATGTG
    TACCCATATG ACCGCTGGAG TGACAAGACC CTCCCTGTCT ATGAGAGAGG CTCCCACTTT
    CAGCCCAGCA CTGTGGAGAT GGTGGACGGA GAGACCAGCC CACCCCAGCT GCTCACCGAA
    GCCGACCTCA TTGCCCTCAT GGAGAAGCAC GGCATTGGTA CTGACGCCAC TCATGCGGAG
    CACATCGAGA CAATCAAAGC CCGGATGTAT GTTGGGCTCA CCGCAGACAA GCGGTTTCTC
    CCTGGACATC TGGGCATGGG ACTTGTGGAA GGCTATGACT CCATGGGCTA TGAGATGTCC
    AAGCCTGACC TCCGGGCCGA GCTAGAAGCT GATCTGAAGC TGATCTGTGA AGGCAAGAAG
    GACAAGTCAG TGGTTCTAAG GCAGCAAGTC CAGAAATACA AGCAGGTTTT CATTGAAGCT
    GTGGCCAAAG CAAAGAAATT GGACGAGGCC TTATCCCAGT ACTTTGGGGA AGGGACAGAG
    GTGGCCCAGC AAGAAGCCAT CTACCCGAGC ATGCCAGAGC CCATCAAGAA GTGTCCGCAG
    TGCAACAAGG ATATGGTCCT CAAGACAAAG AAGAATGGCG GGTTCTACCT CAGCTGCACG
    GGCTTCCCGG AATGTCGCTC AGCCGTGTGG TTCCCTGACT CTGTGCTGGA GGCCAGCAGG
    GATGACAGTG TGTGTCCGGT TTGTCAGCCT CACCCCGTTT ACAGGTTGAA GTTAAAGTTT
    AAACGCGGCA GCCTTCCCCC GACCATGCCC CTGGAGTTTG TTGGCTGCAT CGGCGGATGT
    GACGAGACGC TGCGGGAAAT CTTGGACCTG AGGTTCTCAC GGGGCCCCCC CAGAGCTTCC
    CAGCCAGCTA GCCAGCCCTC TAACTACCTG CAAGCTAGCC AGTCCCAGAA CAGAACGGAC
    AGCGCCCCCC GGATGCTGCC CCTGCCCGCC GCCGCCGATG GGGAAAGTAA TTCTGTGATC
    TGCAACTGTG GCCAGGAGGC CGTGCTGCTC ACTGTGAGGA AGGAGGGTCC CAACCAGGGC
    CGGCAGTTCT ATAAGTGTGG TGGCGGTGGC TGTGACTTCT TCCTGTGGGC TGATAGCAGC
    GCCCTGGAAC CCAGGGGGCC TCCTGCCTCT GCACTGGGGC CTGCGGGCGG CTCGCTGGGC
    CACCCACCTG CTATGGGGGA CCGCCGGGGT GGGTTTGGCA GACCCGGAGA TGATGGTGGC
    CGGGGCATGG CCTGCCTGTG TAGCCAGCCC GCCGTCACCC GGACTGTGCA GAAGGATGGG
    CCCAACAAGG GCCGCCAGTT CCACACGTGC GCCAAGCCGA GAGAGCAGCA GTGCGGCTTC
    TTTCAATGGG TGGACGAGAA TGTGGCCCCA GGGACTCTCA GAACCCCAAC CTGGCCAAGA
    GGCAGAGGAA GAACTCAGGG GCCAGAGGCC AGAAGCAAAA GAGCCCGGGC CAGTTCCGCA
    GACATGGGGT CCACTCCCAA GAAACCCCGG AAATGCAGCC TTTGCCACCA GCCTGGCCAT
    ACCCGTCCCT TCTGTCCTCA GAACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_546656.2
    CDS340..3309
    Translation

    Target ORF information:

    RefSeq Version XM_546656.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DNA topoisomerase III alpha (TOP3A), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_546656.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATCTTCC CGGTCGCCCG CAGCGCGCTC CGCTGGCTGC TACGGCCGGG AGCCCGGACC 
    TTGTCCCGTG CTGCCATGGA GGTGGCCTTC CGCGGTGTGC GCAAAATTCT CTGTGTGGCC
    GAGAAAAACG ACGCGGCCAA GGGGATCGCC GACCTGCTGT CCAACGGCCG CATGAGGCGG
    AGGGAGGGAT TTTCAAAATT CAACAAGATC TATGAATTTG ATTATCATCT ATATGGCCAG
    AATGTTACCA TGGTAATGAC TTCAGTTTCT GGACATTTGC TGGCTCACGA TTTCCAGATG
    CAGTTTCGCA AATGGCAGAG CTGCAATCCC CTTGTCCTCT TTGAAGCAGA AATTGAAAAG
    TACTGCCCAG AGAATTTTGT AGGCATCAAG AAAACTCTGC AGCGAGAGAC TCAGCAATGC
    CAGGCCCTGG TGATCTGGAC CGACTGTGAC AGAGAAGGTG AAAATATCGG GTTTGAAATT
    ATCCACGTAT GCAGGGCTGT GAAGCCAAAC CTGCAGGTGC TGAGAGCCCG TTTCTCTGAG
    ATCACCCCCC GAGCTGTCCG GGTGGCCTGT GAAAACCTGA CTGAGCCCGA TCAGAGAGTG
    AGCGATGCCG TGGATGTGAG GCAGGAGTTG GACCTGAGGA TTGGAGCTGC CTTCACTAGG
    TTTCAAACCC TGAGGCTTCA GAAGATTTTT CCCGAGGTGT TAGCAGAACA GCTGATCAGT
    TATGGCAGCT GCCAGTTCCC AACCCTGGGG TTCGTGGTGG AGAGGTTCAA AGCCATTCAG
    GCTTTTGTGC CAGAAATCTT CCACAAAATT AAAGTAACTC ATGACCACAA AGATGGCATC
    GTAGAATTCA ACTGGAAAAG GCATCGTCTC TTTAACCACA CAGCTTGTCT TGTCCTCTAT
    CAGCTGTGCA TGGAGGATCC CACGGCAACT GTGGTGGAGG TCAGGTCTAA GTCAAAGAGC
    AAATGGAGGC CTCAAGCGTT GGACACTGTG GAGCTTGAGA AGCTGGCTTC TCGAAAGTTG
    AGAATAAATG CTAAAGAAAC CATGAGGATT GCCGAAAAGC TCTATACTCA AGGGTACATC
    AGCTATCCTC GAACTGAAAC AAACATTTTC CCCAAAGACT TAGACCTGAC AGTGCTGGTG
    GAGCAGCAGA CCCCGGATCC ACGCTGGGGG GCCTTTGCCC AGAGCATTCT AGAGCGGGGT
    GGCCCCACTC CACGCAATGG GAACAAGTCT GACCAGGCTC ACCCTCCCAT CCACCCCACC
    AAATACACCA GCAGCTTGCA GGGAGATGAG CAGCGGCTGT ATGAGTTCAT CGTGCGGCAT
    TTCCTGGCTT GCTGCTCCCA GGATGCCCAG GGGCAGGAGA CCACTGTGGA GATTGACATC
    GCTCAGGAAC GCTTCGTGGC CCACGGCCTC ATGATTTTGG CCCGAAACTA TTTGGATGTG
    TACCCATATG ACCGCTGGAG TGACAAGACC CTCCCTGTCT ATGAGAGAGG CTCCCACTTT
    CAGCCCAGCA CTGTGGAGAT GGTGGACGGA GAGACCAGCC CACCCCAGCT GCTCACCGAA
    GCCGACCTCA TTGCCCTCAT GGAGAAGCAC GGCATTGGTA CTGACGCCAC TCATGCGGAG
    CACATCGAGA CAATCAAAGC CCGGATGTAT GTTGGGCTCA CCGCAGACAA GCGGTTTCTC
    CCTGGACATC TGGGCATGGG ACTTGTGGAA GGCTATGACT CCATGGGCTA TGAGATGTCC
    AAGCCTGACC TCCGGGCCGA GCTAGAAGCT GATCTGAAGC TGATCTGTGA AGGCAAGAAG
    GACAAGTCAG TGGTTCTAAG GCAGCAAGTC CAGAAATACA AGCAGGTTTT CATTGAAGCT
    GTGGCCAAAG CAAAGAAATT GGACGAGGCC TTATCCCAGT ACTTTGGGGA AGGGACAGAG
    GTGGCCCAGC AAGAAGCCAT CTACCCGAGC ATGCCAGAGC CCATCAAGAA GTGTCCGCAG
    TGCAACAAGG ATATGGTCCT CAAGACAAAG AAGAATGGCG GGTTCTACCT CAGCTGCACG
    GGCTTCCCGG AATGTCGCTC AGCCGTGTGG TTCCCTGACT CTGTGCTGGA GGCCAGCAGG
    GATGACAGTG TGTGTCCGGT TTGTCAGCCT CACCCCGTTT ACAGGTTGAA GTTAAAGTTT
    AAACGCGGCA GCCTTCCCCC GACCATGCCC CTGGAGTTTG TTGGCTGCAT CGGCGGATGT
    GACGAGACGC TGCGGGAAAT CTTGGACCTG AGGTTCTCAC GGGGCCCCCC CAGAGCTTCC
    CAGCCAGCTA GCCAGCCCTC TAACTACCTG CAAGCTAGCC AGTCCCAGAA CAGAACGGAC
    AGCGCCCCCC GGATGCTGCC CCTGCCCGCC GCCGCCGATG GGGAAAGTAA TTCTGTGATC
    TGCAACTGTG GCCAGGAGGC CGTGCTGCTC ACTGTGAGGA AGGAGGGTCC CAACCAGGGC
    CGGCAGTTCT ATAAGTGTGG TGGCGGTGGC TGTGACTTCT TCCTGTGGGC TGATAGCAGC
    GCCCTGGAAC CCAGGGGGCC TCCTGCCTCT GCACTGGGGC CTGCGGGCGG CTCGCTGGGC
    CACCCACCTG CTATGGGGGA CCGCCGGGGT GGGTTTGGCA GACCCGGAGA TGATGGTGGC
    CGGGGCATGG CCTGCCTGTG TAGCCAGCCC GCCGTCACCC GGACTGTGCA GAAGGATGGG
    CCCAACAAGG GCCGCCAGTT CCACACGTGC GCCAAGCCGA GAGAGCAGCA GTGCGGCTTC
    TTTCAATGGG TGGACGAGAA TGTGGCCCCA GGGACTCTCA GAACCCCAAC CTGGCCAAGA
    GGCAGAGGAA GAACTCAGGG GCCAGAGGCC AGAAGCAAAA GAGCCCGGGC CAGTTCCGCA
    GACATGGGGT CCACTCCCAA GAAACCCCGG AAATGCAGCC TTTGCCACCA GCCTGGCCAT
    ACCCGTCCCT TCTGTCCTCA GAACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06425
    Clone ID Related Accession (Same CDS sequence) XM_546656.6 , XM_546656.5
    Accession Version XM_546656.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2970bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DNA topoisomerase 3-alpha isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_546656.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATCTTCC CGGTCGCCCG CAGCGCGCTC CGCTGGCTGC TACGGCCGGG AGCCCGGACC 
    TTGTCCCGTG CTGCCATGGA GGTGGCCTTC CGCGGTGTGC GCAAAATTCT CTGTGTGGCC
    GAGAAAAACG ACGCGGCCAA GGGGATCGCC GACCTGCTGT CCAACGGCCG CATGAGGCGG
    AGGGAGGGAT TTTCAAAATT CAACAAGATC TATGAATTTG ATTATCATCT ATATGGCCAG
    AATGTTACCA TGGTAATGAC TTCAGTTTCT GGACATTTGC TGGCTCACGA TTTCCAGATG
    CAGTTTCGCA AATGGCAGAG CTGCAATCCC CTTGTCCTCT TTGAAGCAGA AATTGAAAAG
    TACTGCCCAG AGAATTTTGT AGGCATCAAG AAAACTCTGC AGCGAGAGAC TCAGCAATGC
    CAGGCCCTGG TGATCTGGAC CGACTGTGAC AGAGAAGGTG AAAATATCGG GTTTGAAATT
    ATCCACGTAT GCAGGGCTGT GAAGCCAAAC CTGCAGGTGC TGAGAGCCCG TTTCTCTGAG
    ATCACCCCCC GAGCTGTCCG GGTGGCCTGT GAAAACCTGA CTGAGCCCGA TCAGAGAGTG
    AGCGATGCCG TGGATGTGAG GCAGGAGTTG GACCTGAGGA TTGGAGCTGC CTTCACTAGG
    TTTCAAACCC TGAGGCTTCA GAAGATTTTT CCCGAGGTGT TAGCAGAACA GCTGATCAGT
    TATGGCAGCT GCCAGTTCCC AACCCTGGGG TTCGTGGTGG AGAGGTTCAA AGCCATTCAG
    GCTTTTGTGC CAGAAATCTT CCACAAAATT AAAGTAACTC ATGACCACAA AGATGGCATC
    GTAGAATTCA ACTGGAAAAG GCATCGTCTC TTTAACCACA CAGCTTGTCT TGTCCTCTAT
    CAGCTGTGCA TGGAGGATCC CACGGCAACT GTGGTGGAGG TCAGGTCTAA GTCAAAGAGC
    AAATGGAGGC CTCAAGCGTT GGACACTGTG GAGCTTGAGA AGCTGGCTTC TCGAAAGTTG
    AGAATAAATG CTAAAGAAAC CATGAGGATT GCCGAAAAGC TCTATACTCA AGGGTACATC
    AGCTATCCTC GAACTGAAAC AAACATTTTC CCCAAAGACT TAGACCTGAC AGTGCTGGTG
    GAGCAGCAGA CCCCGGATCC ACGCTGGGGG GCCTTTGCCC AGAGCATTCT AGAGCGGGGT
    GGCCCCACTC CACGCAATGG GAACAAGTCT GACCAGGCTC ACCCTCCCAT CCACCCCACC
    AAATACACCA GCAGCTTGCA GGGAGATGAG CAGCGGCTGT ATGAGTTCAT CGTGCGGCAT
    TTCCTGGCTT GCTGCTCCCA GGATGCCCAG GGGCAGGAGA CCACTGTGGA GATTGACATC
    GCTCAGGAAC GCTTCGTGGC CCACGGCCTC ATGATTTTGG CCCGAAACTA TTTGGATGTG
    TACCCATATG ACCGCTGGAG TGACAAGACC CTCCCTGTCT ATGAGAGAGG CTCCCACTTT
    CAGCCCAGCA CTGTGGAGAT GGTGGACGGA GAGACCAGCC CACCCCAGCT GCTCACCGAA
    GCCGACCTCA TTGCCCTCAT GGAGAAGCAC GGCATTGGTA CTGACGCCAC TCATGCGGAG
    CACATCGAGA CAATCAAAGC CCGGATGTAT GTTGGGCTCA CCGCAGACAA GCGGTTTCTC
    CCTGGACATC TGGGCATGGG ACTTGTGGAA GGCTATGACT CCATGGGCTA TGAGATGTCC
    AAGCCTGACC TCCGGGCCGA GCTAGAAGCT GATCTGAAGC TGATCTGTGA AGGCAAGAAG
    GACAAGTCAG TGGTTCTAAG GCAGCAAGTC CAGAAATACA AGCAGGTTTT CATTGAAGCT
    GTGGCCAAAG CAAAGAAATT GGACGAGGCC TTATCCCAGT ACTTTGGGGA AGGGACAGAG
    GTGGCCCAGC AAGAAGCCAT CTACCCGAGC ATGCCAGAGC CCATCAAGAA GTGTCCGCAG
    TGCAACAAGG ATATGGTCCT CAAGACAAAG AAGAATGGCG GGTTCTACCT CAGCTGCACG
    GGCTTCCCGG AATGTCGCTC AGCCGTGTGG TTCCCTGACT CTGTGCTGGA GGCCAGCAGG
    GATGACAGTG TGTGTCCGGT TTGTCAGCCT CACCCCGTTT ACAGGTTGAA GTTAAAGTTT
    AAACGCGGCA GCCTTCCCCC GACCATGCCC CTGGAGTTTG TTGGCTGCAT CGGCGGATGT
    GACGAGACGC TGCGGGAAAT CTTGGACCTG AGGTTCTCAC GGGGCCCCCC CAGAGCTTCC
    CAGCCAGCTA GCCAGCCCTC TAACTACCTG CAAGCTAGCC AGTCCCAGAA CAGAACGGAC
    AGCGCCCCCC GGATGCTGCC CCTGCCCGCC GCCGCCGATG GGGAAAGTAA TTCTGTGATC
    TGCAACTGTG GCCAGGAGGC CGTGCTGCTC ACTGTGAGGA AGGAGGGTCC CAACCAGGGC
    CGGCAGTTCT ATAAGTGTGG TGGCGGTGGC TGTGACTTCT TCCTGTGGGC TGATAGCAGC
    GCCCTGGAAC CCAGGGGGCC TCCTGCCTCT GCACTGGGGC CTGCGGGCGG CTCGCTGGGC
    CACCCACCTG CTATGGGGGA CCGCCGGGGT GGGTTTGGCA GACCCGGAGA TGATGGTGGC
    CGGGGCATGG CCTGCCTGTG TAGCCAGCCC GCCGTCACCC GGACTGTGCA GAAGGATGGG
    CCCAACAAGG GCCGCCAGTT CCACACGTGC GCCAAGCCGA GAGAGCAGCA GTGCGGCTTC
    TTTCAATGGG TGGACGAGAA TGTGGCCCCA GGGACTCTCA GAACCCCAAC CTGGCCAAGA
    GGCAGAGGAA GAACTCAGGG GCCAGAGGCC AGAAGCAAAA GAGCCCGGGC CAGTTCCGCA
    GACATGGGGT CCACTCCCAA GAAACCCCGG AAATGCAGCC TTTGCCACCA GCCTGGCCAT
    ACCCGTCCCT TCTGTCCTCA GAACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_546656.2
    CDS206..3175
    Translation

    Target ORF information:

    RefSeq Version XM_546656.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris topoisomerase (DNA) III alpha (TOP3A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_546656.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATCTTCC CGGTCGCCCG CAGCGCGCTC CGCTGGCTGC TACGGCCGGG AGCCCGGACC 
    TTGTCCCGTG CTGCCATGGA GGTGGCCTTC CGCGGTGTGC GCAAAATTCT CTGTGTGGCC
    GAGAAAAACG ACGCGGCCAA GGGGATCGCC GACCTGCTGT CCAACGGCCG CATGAGGCGG
    AGGGAGGGAT TTTCAAAATT CAACAAGATC TATGAATTTG ATTATCATCT ATATGGCCAG
    AATGTTACCA TGGTAATGAC TTCAGTTTCT GGACATTTGC TGGCTCACGA TTTCCAGATG
    CAGTTTCGCA AATGGCAGAG CTGCAATCCC CTTGTCCTCT TTGAAGCAGA AATTGAAAAG
    TACTGCCCAG AGAATTTTGT AGGCATCAAG AAAACTCTGC AGCGAGAGAC TCAGCAATGC
    CAGGCCCTGG TGATCTGGAC CGACTGTGAC AGAGAAGGTG AAAATATCGG GTTTGAAATT
    ATCCACGTAT GCAGGGCTGT GAAGCCAAAC CTGCAGGTGC TGAGAGCCCG TTTCTCTGAG
    ATCACCCCCC GAGCTGTCCG GGTGGCCTGT GAAAACCTGA CTGAGCCCGA TCAGAGAGTG
    AGCGATGCCG TGGATGTGAG GCAGGAGTTG GACCTGAGGA TTGGAGCTGC CTTCACTAGG
    TTTCAAACCC TGAGGCTTCA GAAGATTTTT CCCGAGGTGT TAGCAGAACA GCTGATCAGT
    TATGGCAGCT GCCAGTTCCC AACCCTGGGG TTCGTGGTGG AGAGGTTCAA AGCCATTCAG
    GCTTTTGTGC CAGAAATCTT CCACAAAATT AAAGTAACTC ATGACCACAA AGATGGCATC
    GTAGAATTCA ACTGGAAAAG GCATCGTCTC TTTAACCACA CAGCTTGTCT TGTCCTCTAT
    CAGCTGTGCA TGGAGGATCC CACGGCAACT GTGGTGGAGG TCAGGTCTAA GTCAAAGAGC
    AAATGGAGGC CTCAAGCGTT GGACACTGTG GAGCTTGAGA AGCTGGCTTC TCGAAAGTTG
    AGAATAAATG CTAAAGAAAC CATGAGGATT GCCGAAAAGC TCTATACTCA AGGGTACATC
    AGCTATCCTC GAACTGAAAC AAACATTTTC CCCAAAGACT TAGACCTGAC AGTGCTGGTG
    GAGCAGCAGA CCCCGGATCC ACGCTGGGGG GCCTTTGCCC AGAGCATTCT AGAGCGGGGT
    GGCCCCACTC CACGCAATGG GAACAAGTCT GACCAGGCTC ACCCTCCCAT CCACCCCACC
    AAATACACCA GCAGCTTGCA GGGAGATGAG CAGCGGCTGT ATGAGTTCAT CGTGCGGCAT
    TTCCTGGCTT GCTGCTCCCA GGATGCCCAG GGGCAGGAGA CCACTGTGGA GATTGACATC
    GCTCAGGAAC GCTTCGTGGC CCACGGCCTC ATGATTTTGG CCCGAAACTA TTTGGATGTG
    TACCCATATG ACCGCTGGAG TGACAAGACC CTCCCTGTCT ATGAGAGAGG CTCCCACTTT
    CAGCCCAGCA CTGTGGAGAT GGTGGACGGA GAGACCAGCC CACCCCAGCT GCTCACCGAA
    GCCGACCTCA TTGCCCTCAT GGAGAAGCAC GGCATTGGTA CTGACGCCAC TCATGCGGAG
    CACATCGAGA CAATCAAAGC CCGGATGTAT GTTGGGCTCA CCGCAGACAA GCGGTTTCTC
    CCTGGACATC TGGGCATGGG ACTTGTGGAA GGCTATGACT CCATGGGCTA TGAGATGTCC
    AAGCCTGACC TCCGGGCCGA GCTAGAAGCT GATCTGAAGC TGATCTGTGA AGGCAAGAAG
    GACAAGTCAG TGGTTCTAAG GCAGCAAGTC CAGAAATACA AGCAGGTTTT CATTGAAGCT
    GTGGCCAAAG CAAAGAAATT GGACGAGGCC TTATCCCAGT ACTTTGGGGA AGGGACAGAG
    GTGGCCCAGC AAGAAGCCAT CTACCCGAGC ATGCCAGAGC CCATCAAGAA GTGTCCGCAG
    TGCAACAAGG ATATGGTCCT CAAGACAAAG AAGAATGGCG GGTTCTACCT CAGCTGCACG
    GGCTTCCCGG AATGTCGCTC AGCCGTGTGG TTCCCTGACT CTGTGCTGGA GGCCAGCAGG
    GATGACAGTG TGTGTCCGGT TTGTCAGCCT CACCCCGTTT ACAGGTTGAA GTTAAAGTTT
    AAACGCGGCA GCCTTCCCCC GACCATGCCC CTGGAGTTTG TTGGCTGCAT CGGCGGATGT
    GACGAGACGC TGCGGGAAAT CTTGGACCTG AGGTTCTCAC GGGGCCCCCC CAGAGCTTCC
    CAGCCAGCTA GCCAGCCCTC TAACTACCTG CAAGCTAGCC AGTCCCAGAA CAGAACGGAC
    AGCGCCCCCC GGATGCTGCC CCTGCCCGCC GCCGCCGATG GGGAAAGTAA TTCTGTGATC
    TGCAACTGTG GCCAGGAGGC CGTGCTGCTC ACTGTGAGGA AGGAGGGTCC CAACCAGGGC
    CGGCAGTTCT ATAAGTGTGG TGGCGGTGGC TGTGACTTCT TCCTGTGGGC TGATAGCAGC
    GCCCTGGAAC CCAGGGGGCC TCCTGCCTCT GCACTGGGGC CTGCGGGCGG CTCGCTGGGC
    CACCCACCTG CTATGGGGGA CCGCCGGGGT GGGTTTGGCA GACCCGGAGA TGATGGTGGC
    CGGGGCATGG CCTGCCTGTG TAGCCAGCCC GCCGTCACCC GGACTGTGCA GAAGGATGGG
    CCCAACAAGG GCCGCCAGTT CCACACGTGC GCCAAGCCGA GAGAGCAGCA GTGCGGCTTC
    TTTCAATGGG TGGACGAGAA TGTGGCCCCA GGGACTCTCA GAACCCCAAC CTGGCCAAGA
    GGCAGAGGAA GAACTCAGGG GCCAGAGGCC AGAAGCAAAA GAGCCCGGGC CAGTTCCGCA
    GACATGGGGT CCACTCCCAA GAAACCCCGG AAATGCAGCC TTTGCCACCA GCCTGGCCAT
    ACCCGTCCCT TCTGTCCTCA GAACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06426
    Clone ID Related Accession (Same CDS sequence) XM_005620144.2
    Accession Version XM_005620144.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2910bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DNA topoisomerase 3-alpha isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620144.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGATCTTCC CGGTCGCCCG CAGCGCGCTC CGCTGGCTGC TACGGCCGGG AGCCCGGACC 
    TTGTCCCGTG CTGCCATGGA GGTGGCCTTC CGCGGTGTGC GCAAAATTCT CTGTGTGGCC
    GAGAAAAACG ACGCGGCCAA GGGGATCGCC GACCTGCTGT CCAACGGCCG CATGAGGCGG
    AATGTTACCA TGGTAATGAC TTCAGTTTCT GGACATTTGC TGGCTCACGA TTTCCAGATG
    CAGTTTCGCA AATGGCAGAG CTGCAATCCC CTTGTCCTCT TTGAAGCAGA AATTGAAAAG
    TACTGCCCAG AGAATTTTGT AGGCATCAAG AAAACTCTGC AGCGAGAGAC TCAGCAATGC
    CAGGCCCTGG TGATCTGGAC CGACTGTGAC AGAGAAGGTG AAAATATCGG GTTTGAAATT
    ATCCACGTAT GCAGGGCTGT GAAGCCAAAC CTGCAGGTGC TGAGAGCCCG TTTCTCTGAG
    ATCACCCCCC GAGCTGTCCG GGTGGCCTGT GAAAACCTGA CTGAGCCCGA TCAGAGAGTG
    AGCGATGCCG TGGATGTGAG GCAGGAGTTG GACCTGAGGA TTGGAGCTGC CTTCACTAGG
    TTTCAAACCC TGAGGCTTCA GAAGATTTTT CCCGAGGTGT TAGCAGAACA GCTGATCAGT
    TATGGCAGCT GCCAGTTCCC AACCCTGGGG TTCGTGGTGG AGAGGTTCAA AGCCATTCAG
    GCTTTTGTGC CAGAAATCTT CCACAAAATT AAAGTAACTC ATGACCACAA AGATGGCATC
    GTAGAATTCA ACTGGAAAAG GCATCGTCTC TTTAACCACA CAGCTTGTCT TGTCCTCTAT
    CAGCTGTGCA TGGAGGATCC CACGGCAACT GTGGTGGAGG TCAGGTCTAA GTCAAAGAGC
    AAATGGAGGC CTCAAGCGTT GGACACTGTG GAGCTTGAGA AGCTGGCTTC TCGAAAGTTG
    AGAATAAATG CTAAAGAAAC CATGAGGATT GCCGAAAAGC TCTATACTCA AGGGTACATC
    AGCTATCCTC GAACTGAAAC AAACATTTTC CCCAAAGACT TAGACCTGAC AGTGCTGGTG
    GAGCAGCAGA CCCCGGATCC ACGCTGGGGG GCCTTTGCCC AGAGCATTCT AGAGCGGGGT
    GGCCCCACTC CACGCAATGG GAACAAGTCT GACCAGGCTC ACCCTCCCAT CCACCCCACC
    AAATACACCA GCAGCTTGCA GGGAGATGAG CAGCGGCTGT ATGAGTTCAT CGTGCGGCAT
    TTCCTGGCTT GCTGCTCCCA GGATGCCCAG GGGCAGGAGA CCACTGTGGA GATTGACATC
    GCTCAGGAAC GCTTCGTGGC CCACGGCCTC ATGATTTTGG CCCGAAACTA TTTGGATGTG
    TACCCATATG ACCGCTGGAG TGACAAGACC CTCCCTGTCT ATGAGAGAGG CTCCCACTTT
    CAGCCCAGCA CTGTGGAGAT GGTGGACGGA GAGACCAGCC CACCCCAGCT GCTCACCGAA
    GCCGACCTCA TTGCCCTCAT GGAGAAGCAC GGCATTGGTA CTGACGCCAC TCATGCGGAG
    CACATCGAGA CAATCAAAGC CCGGATGTAT GTTGGGCTCA CCGCAGACAA GCGGTTTCTC
    CCTGGACATC TGGGCATGGG ACTTGTGGAA GGCTATGACT CCATGGGCTA TGAGATGTCC
    AAGCCTGACC TCCGGGCCGA GCTAGAAGCT GATCTGAAGC TGATCTGTGA AGGCAAGAAG
    GACAAGTCAG TGGTTCTAAG GCAGCAAGTC CAGAAATACA AGCAGGTTTT CATTGAAGCT
    GTGGCCAAAG CAAAGAAATT GGACGAGGCC TTATCCCAGT ACTTTGGGGA AGGGACAGAG
    GTGGCCCAGC AAGAAGCCAT CTACCCGAGC ATGCCAGAGC CCATCAAGAA GTGTCCGCAG
    TGCAACAAGG ATATGGTCCT CAAGACAAAG AAGAATGGCG GGTTCTACCT CAGCTGCACG
    GGCTTCCCGG AATGTCGCTC AGCCGTGTGG TTCCCTGACT CTGTGCTGGA GGCCAGCAGG
    GATGACAGTG TGTGTCCGGT TTGTCAGCCT CACCCCGTTT ACAGGTTGAA GTTAAAGTTT
    AAACGCGGCA GCCTTCCCCC GACCATGCCC CTGGAGTTTG TTGGCTGCAT CGGCGGATGT
    GACGAGACGC TGCGGGAAAT CTTGGACCTG AGGTTCTCAC GGGGCCCCCC CAGAGCTTCC
    CAGCCAGCTA GCCAGCCCTC TAACTACCTG CAAGCTAGCC AGTCCCAGAA CAGAACGGAC
    AGCGCCCCCC GGATGCTGCC CCTGCCCGCC GCCGCCGATG GGGAAAGTAA TTCTGTGATC
    TGCAACTGTG GCCAGGAGGC CGTGCTGCTC ACTGTGAGGA AGGAGGGTCC CAACCAGGGC
    CGGCAGTTCT ATAAGTGTGG TGGCGGTGGC TGTGACTTCT TCCTGTGGGC TGATAGCAGC
    GCCCTGGAAC CCAGGGGGCC TCCTGCCTCT GCACTGGGGC CTGCGGGCGG CTCGCTGGGC
    CACCCACCTG CTATGGGGGA CCGCCGGGGT GGGTTTGGCA GACCCGGAGA TGATGGTGGC
    CGGGGCATGG CCTGCCTGTG TAGCCAGCCC GCCGTCACCC GGACTGTGCA GAAGGATGGG
    CCCAACAAGG GCCGCCAGTT CCACACGTGC GCCAAGCCGA GAGAGCAGCA GTGCGGCTTC
    TTTCAATGGG TGGACGAGAA TGTGGCCCCA GGGACTCTCA GAACCCCAAC CTGGCCAAGA
    GGCAGAGGAA GAACTCAGGG GCCAGAGGCC AGAAGCAAAA GAGCCCGGGC CAGTTCCGCA
    GACATGGGGT CCACTCCCAA GAAACCCCGG AAATGCAGCC TTTGCCACCA GCCTGGCCAT
    ACCCGTCCCT TCTGTCCTCA GAACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620201.1
    CDS206..3115
    Translation

    Target ORF information:

    RefSeq Version XM_005620144.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris topoisomerase (DNA) III alpha (TOP3A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620144.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGATCTTCC CGGTCGCCCG CAGCGCGCTC CGCTGGCTGC TACGGCCGGG AGCCCGGACC 
    TTGTCCCGTG CTGCCATGGA GGTGGCCTTC CGCGGTGTGC GCAAAATTCT CTGTGTGGCC
    GAGAAAAACG ACGCGGCCAA GGGGATCGCC GACCTGCTGT CCAACGGCCG CATGAGGCGG
    AATGTTACCA TGGTAATGAC TTCAGTTTCT GGACATTTGC TGGCTCACGA TTTCCAGATG
    CAGTTTCGCA AATGGCAGAG CTGCAATCCC CTTGTCCTCT TTGAAGCAGA AATTGAAAAG
    TACTGCCCAG AGAATTTTGT AGGCATCAAG AAAACTCTGC AGCGAGAGAC TCAGCAATGC
    CAGGCCCTGG TGATCTGGAC CGACTGTGAC AGAGAAGGTG AAAATATCGG GTTTGAAATT
    ATCCACGTAT GCAGGGCTGT GAAGCCAAAC CTGCAGGTGC TGAGAGCCCG TTTCTCTGAG
    ATCACCCCCC GAGCTGTCCG GGTGGCCTGT GAAAACCTGA CTGAGCCCGA TCAGAGAGTG
    AGCGATGCCG TGGATGTGAG GCAGGAGTTG GACCTGAGGA TTGGAGCTGC CTTCACTAGG
    TTTCAAACCC TGAGGCTTCA GAAGATTTTT CCCGAGGTGT TAGCAGAACA GCTGATCAGT
    TATGGCAGCT GCCAGTTCCC AACCCTGGGG TTCGTGGTGG AGAGGTTCAA AGCCATTCAG
    GCTTTTGTGC CAGAAATCTT CCACAAAATT AAAGTAACTC ATGACCACAA AGATGGCATC
    GTAGAATTCA ACTGGAAAAG GCATCGTCTC TTTAACCACA CAGCTTGTCT TGTCCTCTAT
    CAGCTGTGCA TGGAGGATCC CACGGCAACT GTGGTGGAGG TCAGGTCTAA GTCAAAGAGC
    AAATGGAGGC CTCAAGCGTT GGACACTGTG GAGCTTGAGA AGCTGGCTTC TCGAAAGTTG
    AGAATAAATG CTAAAGAAAC CATGAGGATT GCCGAAAAGC TCTATACTCA AGGGTACATC
    AGCTATCCTC GAACTGAAAC AAACATTTTC CCCAAAGACT TAGACCTGAC AGTGCTGGTG
    GAGCAGCAGA CCCCGGATCC ACGCTGGGGG GCCTTTGCCC AGAGCATTCT AGAGCGGGGT
    GGCCCCACTC CACGCAATGG GAACAAGTCT GACCAGGCTC ACCCTCCCAT CCACCCCACC
    AAATACACCA GCAGCTTGCA GGGAGATGAG CAGCGGCTGT ATGAGTTCAT CGTGCGGCAT
    TTCCTGGCTT GCTGCTCCCA GGATGCCCAG GGGCAGGAGA CCACTGTGGA GATTGACATC
    GCTCAGGAAC GCTTCGTGGC CCACGGCCTC ATGATTTTGG CCCGAAACTA TTTGGATGTG
    TACCCATATG ACCGCTGGAG TGACAAGACC CTCCCTGTCT ATGAGAGAGG CTCCCACTTT
    CAGCCCAGCA CTGTGGAGAT GGTGGACGGA GAGACCAGCC CACCCCAGCT GCTCACCGAA
    GCCGACCTCA TTGCCCTCAT GGAGAAGCAC GGCATTGGTA CTGACGCCAC TCATGCGGAG
    CACATCGAGA CAATCAAAGC CCGGATGTAT GTTGGGCTCA CCGCAGACAA GCGGTTTCTC
    CCTGGACATC TGGGCATGGG ACTTGTGGAA GGCTATGACT CCATGGGCTA TGAGATGTCC
    AAGCCTGACC TCCGGGCCGA GCTAGAAGCT GATCTGAAGC TGATCTGTGA AGGCAAGAAG
    GACAAGTCAG TGGTTCTAAG GCAGCAAGTC CAGAAATACA AGCAGGTTTT CATTGAAGCT
    GTGGCCAAAG CAAAGAAATT GGACGAGGCC TTATCCCAGT ACTTTGGGGA AGGGACAGAG
    GTGGCCCAGC AAGAAGCCAT CTACCCGAGC ATGCCAGAGC CCATCAAGAA GTGTCCGCAG
    TGCAACAAGG ATATGGTCCT CAAGACAAAG AAGAATGGCG GGTTCTACCT CAGCTGCACG
    GGCTTCCCGG AATGTCGCTC AGCCGTGTGG TTCCCTGACT CTGTGCTGGA GGCCAGCAGG
    GATGACAGTG TGTGTCCGGT TTGTCAGCCT CACCCCGTTT ACAGGTTGAA GTTAAAGTTT
    AAACGCGGCA GCCTTCCCCC GACCATGCCC CTGGAGTTTG TTGGCTGCAT CGGCGGATGT
    GACGAGACGC TGCGGGAAAT CTTGGACCTG AGGTTCTCAC GGGGCCCCCC CAGAGCTTCC
    CAGCCAGCTA GCCAGCCCTC TAACTACCTG CAAGCTAGCC AGTCCCAGAA CAGAACGGAC
    AGCGCCCCCC GGATGCTGCC CCTGCCCGCC GCCGCCGATG GGGAAAGTAA TTCTGTGATC
    TGCAACTGTG GCCAGGAGGC CGTGCTGCTC ACTGTGAGGA AGGAGGGTCC CAACCAGGGC
    CGGCAGTTCT ATAAGTGTGG TGGCGGTGGC TGTGACTTCT TCCTGTGGGC TGATAGCAGC
    GCCCTGGAAC CCAGGGGGCC TCCTGCCTCT GCACTGGGGC CTGCGGGCGG CTCGCTGGGC
    CACCCACCTG CTATGGGGGA CCGCCGGGGT GGGTTTGGCA GACCCGGAGA TGATGGTGGC
    CGGGGCATGG CCTGCCTGTG TAGCCAGCCC GCCGTCACCC GGACTGTGCA GAAGGATGGG
    CCCAACAAGG GCCGCCAGTT CCACACGTGC GCCAAGCCGA GAGAGCAGCA GTGCGGCTTC
    TTTCAATGGG TGGACGAGAA TGTGGCCCCA GGGACTCTCA GAACCCCAAC CTGGCCAAGA
    GGCAGAGGAA GAACTCAGGG GCCAGAGGCC AGAAGCAAAA GAGCCCGGGC CAGTTCCGCA
    GACATGGGGT CCACTCCCAA GAAACCCCGG AAATGCAGCC TTTGCCACCA GCCTGGCCAT
    ACCCGTCCCT TCTGTCCTCA GAACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36494
    Clone ID Related Accession (Same CDS sequence) XM_014113658.1
    Accession Version XM_014113658.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DNA topoisomerase 3-alpha isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGATCTTCC CGGTCGCCCG CAGCGCGCTC CGCTGGCTGC TACGGCCGGG AGCCCGGACC 
    TTGTCCCGTG CTGCCATGGA GGTGGCCTTC CGCGGTGTGC GCAAAATTCT CTGTGTGGCC
    GAGAAAAACG ACGCGGCCAA GGGGATCGCC GACCTGCTGT CCAACGGCCG CATGAGGCGG
    AAAACTCTGC AGCGAGAGAC TCAGCAATGC CAGGCCCTGG TGATCTGGAC CGACTGTGAC
    AGAGAAGGTG AAAATATCGG GTTTGAAATT ATCCACGTAT GCAGGGCTGT GAAGCCAAAC
    CTGCAGGTGC TGAGAGCCCG TTTCTCTGAG ATCACCCCCC GAGCTGTCCG GGTGGCCTGT
    GAAAACCTGA CTGAGCCCGA TCAGAGAGTG AGCGATGCCG TGGATGTGAG GCAGGAGTTG
    GACCTGAGGA TTGGAGCTGC CTTCACTAGG TTTCAAACCC TGAGGCTTCA GAAGATTTTT
    CCCGAGGTGT TAGCAGAACA GCTGATCAGT TATGGCAGCT GCCAGTTCCC AACCCTGGGG
    TTCGTGGTGG AGAGGTTCAA AGCCATTCAG GCTTTTGTGC CAGAAATCTT CCACAAAATT
    AAAGTAACTC ATGACCACAA AGATGGCATC GTAGAATTCA ACTGGAAAAG GCATCGTCTC
    TTTAACCACA CAGCTTGTCT TGTCCTCTAT CAGCTGTGCA TGGAGGATCC CACGGCAACT
    GTGGTGGAGG TCAGGTCTAA GTCAAAGAGC AAATGGAGGC CTCAAGCGTT GGACACTGTG
    GAGCTTGAGA AGCTGGCTTC TCGAAAGTTG AGAATAAATG CTAAAGAAAC CATGAGGATT
    GCCGAAAAGC TCTATACTCA AGGGTACATC AGCTATCCTC GAACTGAAAC AAACATTTTC
    CCCAAAGACT TAGACCTGAC AGTGCTGGTG GAGCAGCAGA CCCCGGATCC ACGCTGGGGG
    GCCTTTGCCC AGAGCATTCT AGAGCGGGGT GGCCCCACTC CACGCAATGG GAACAAGTCT
    GACCAGGCTC ACCCTCCCAT CCACCCCACC AAATACACCA GCAGCTTGCA GGGAGATGAG
    CAGCGGCTGT ATGAGTTCAT CGTGCGGCAT TTCCTGGCTT GCTGCTCCCA GGATGCCCAG
    GGGCAGGAGA CCACTGTGGA GATTGACATC GCTCAGGAAC GCTTCGTGGC CCACGGCCTC
    ATGATTTTGG CCCGAAACTA TTTGGATGTG TACCCATATG ACCGCTGGAG TGACAAGACC
    CTCCCTGTCT ATGAGAGAGG CTCCCACTTT CAGCCCAGCA CTGTGGAGAT GGTGGACGGA
    GAGACCAGCC CACCCCAGCT GCTCACCGAA GCCGACCTCA TTGCCCTCAT GGAGAAGCAC
    GGCATTGGTA CTGACGCCAC TCATGCGGAG CACATCGAGA CAATCAAAGC CCGGATGTAT
    GTTGGGCTCA CCGCAGACAA GCGGTTTCTC CCTGGACATC TGGGCATGGG ACTTGTGGAA
    GGCTATGACT CCATGGGCTA TGAGATGTCC AAGCCTGACC TCCGGGCCGA GCTAGAAGCT
    GATCTGAAGC TGATCTGTGA AGGCAAGAAG GACAAGTCAG TGGTTCTAAG GCAGCAAGTC
    CAGAAATACA AGCAGGTTTT CATTGAAGCT GTGGCCAAAG CAAAGAAATT GGACGAGGCC
    TTATCCCAGT ACTTTGGGGA AGGGACAGAG GTGGCCCAGC AAGAAGCCAT CTACCCGAGC
    ATGCCAGAGC CCATCAAGAA GTGTCCGCAG TGCAACAAGG ATATGGTCCT CAAGACAAAG
    AAGAATGGCG GGTTCTACCT CAGCTGCACG GGCTTCCCGG AATGTCGCTC AGCCGTGTGG
    TTCCCTGACT CTGTGCTGGA GGCCAGCAGG GATGACAGTG TGTGTCCGGT TTGTCAGCCT
    CACCCCGTTT ACAGGTTGAA GTTAAAGTTT AAACGCGGCA GCCTTCCCCC GACCATGCCC
    CTGGAGTTTG TTGGCTGCAT CGGCGGATGT GACGAGACGC TGCGGGAAAT CTTGGACCTG
    AGGTTCTCAC GGGGCCCCCC CAGAGCTTCC CAGCCAGCTA GCCAGCCCTC TAACTACCTG
    CAAGCTAGCC AGTCCCAGAA CAGAACGGAC AGCGCCCCCC GGATGCTGCC CCTGCCCGCC
    GCCGCCGATG GGGAAAGTAA TTCTGTGATC TGCAACTGTG GCCAGGAGGC CGTGCTGCTC
    ACTGTGAGGA AGGAGGGTCC CAACCAGGGC CGGCAGTTCT ATAAGTGTGG TGGCGGTGGC
    TGTGACTTCT TCCTGTGGGC TGATAGCAGC GCCCTGGAAC CCAGGGGGCC TCCTGCCTCT
    GCACTGGGGC CTGCGGGCGG CTCGCTGGGC CACCCACCTG CTATGGGGGA CCGCCGGGGT
    GGGTTTGGCA GACCCGGAGA TGATGGTGGC CGGGGCATGG CCTGCCTGTG TAGCCAGCCC
    GCCGTCACCC GGACTGTGCA GAAGGATGGG CCCAACAAGG GCCGCCAGTT CCACACGTGC
    GCCAAGCCGA GAGAGCAGCA GTGCGGCTTC TTTCAATGGG TGGACGAGAA TGTGGCCCCA
    GGGACTCTCA GAACCCCAAC CTGGCCAAGA GGCAGAGGAA GAACTCAGGG GCCAGAGGCC
    AGAAGCAAAA GAGCCCGGGC CAGTTCCGCA GACATGGGGT CCACTCCCAA GAAACCCCGG
    AAATGCAGCC TTTGCCACCA GCCTGGCCAT ACCCGTCCCT TCTGTCCTCA GAACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969133.1
    CDS206..2965
    Translation

    Target ORF information:

    RefSeq Version XM_014113658.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris topoisomerase (DNA) III alpha (TOP3A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113658.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGATCTTCC CGGTCGCCCG CAGCGCGCTC CGCTGGCTGC TACGGCCGGG AGCCCGGACC 
    TTGTCCCGTG CTGCCATGGA GGTGGCCTTC CGCGGTGTGC GCAAAATTCT CTGTGTGGCC
    GAGAAAAACG ACGCGGCCAA GGGGATCGCC GACCTGCTGT CCAACGGCCG CATGAGGCGG
    AAAACTCTGC AGCGAGAGAC TCAGCAATGC CAGGCCCTGG TGATCTGGAC CGACTGTGAC
    AGAGAAGGTG AAAATATCGG GTTTGAAATT ATCCACGTAT GCAGGGCTGT GAAGCCAAAC
    CTGCAGGTGC TGAGAGCCCG TTTCTCTGAG ATCACCCCCC GAGCTGTCCG GGTGGCCTGT
    GAAAACCTGA CTGAGCCCGA TCAGAGAGTG AGCGATGCCG TGGATGTGAG GCAGGAGTTG
    GACCTGAGGA TTGGAGCTGC CTTCACTAGG TTTCAAACCC TGAGGCTTCA GAAGATTTTT
    CCCGAGGTGT TAGCAGAACA GCTGATCAGT TATGGCAGCT GCCAGTTCCC AACCCTGGGG
    TTCGTGGTGG AGAGGTTCAA AGCCATTCAG GCTTTTGTGC CAGAAATCTT CCACAAAATT
    AAAGTAACTC ATGACCACAA AGATGGCATC GTAGAATTCA ACTGGAAAAG GCATCGTCTC
    TTTAACCACA CAGCTTGTCT TGTCCTCTAT CAGCTGTGCA TGGAGGATCC CACGGCAACT
    GTGGTGGAGG TCAGGTCTAA GTCAAAGAGC AAATGGAGGC CTCAAGCGTT GGACACTGTG
    GAGCTTGAGA AGCTGGCTTC TCGAAAGTTG AGAATAAATG CTAAAGAAAC CATGAGGATT
    GCCGAAAAGC TCTATACTCA AGGGTACATC AGCTATCCTC GAACTGAAAC AAACATTTTC
    CCCAAAGACT TAGACCTGAC AGTGCTGGTG GAGCAGCAGA CCCCGGATCC ACGCTGGGGG
    GCCTTTGCCC AGAGCATTCT AGAGCGGGGT GGCCCCACTC CACGCAATGG GAACAAGTCT
    GACCAGGCTC ACCCTCCCAT CCACCCCACC AAATACACCA GCAGCTTGCA GGGAGATGAG
    CAGCGGCTGT ATGAGTTCAT CGTGCGGCAT TTCCTGGCTT GCTGCTCCCA GGATGCCCAG
    GGGCAGGAGA CCACTGTGGA GATTGACATC GCTCAGGAAC GCTTCGTGGC CCACGGCCTC
    ATGATTTTGG CCCGAAACTA TTTGGATGTG TACCCATATG ACCGCTGGAG TGACAAGACC
    CTCCCTGTCT ATGAGAGAGG CTCCCACTTT CAGCCCAGCA CTGTGGAGAT GGTGGACGGA
    GAGACCAGCC CACCCCAGCT GCTCACCGAA GCCGACCTCA TTGCCCTCAT GGAGAAGCAC
    GGCATTGGTA CTGACGCCAC TCATGCGGAG CACATCGAGA CAATCAAAGC CCGGATGTAT
    GTTGGGCTCA CCGCAGACAA GCGGTTTCTC CCTGGACATC TGGGCATGGG ACTTGTGGAA
    GGCTATGACT CCATGGGCTA TGAGATGTCC AAGCCTGACC TCCGGGCCGA GCTAGAAGCT
    GATCTGAAGC TGATCTGTGA AGGCAAGAAG GACAAGTCAG TGGTTCTAAG GCAGCAAGTC
    CAGAAATACA AGCAGGTTTT CATTGAAGCT GTGGCCAAAG CAAAGAAATT GGACGAGGCC
    TTATCCCAGT ACTTTGGGGA AGGGACAGAG GTGGCCCAGC AAGAAGCCAT CTACCCGAGC
    ATGCCAGAGC CCATCAAGAA GTGTCCGCAG TGCAACAAGG ATATGGTCCT CAAGACAAAG
    AAGAATGGCG GGTTCTACCT CAGCTGCACG GGCTTCCCGG AATGTCGCTC AGCCGTGTGG
    TTCCCTGACT CTGTGCTGGA GGCCAGCAGG GATGACAGTG TGTGTCCGGT TTGTCAGCCT
    CACCCCGTTT ACAGGTTGAA GTTAAAGTTT AAACGCGGCA GCCTTCCCCC GACCATGCCC
    CTGGAGTTTG TTGGCTGCAT CGGCGGATGT GACGAGACGC TGCGGGAAAT CTTGGACCTG
    AGGTTCTCAC GGGGCCCCCC CAGAGCTTCC CAGCCAGCTA GCCAGCCCTC TAACTACCTG
    CAAGCTAGCC AGTCCCAGAA CAGAACGGAC AGCGCCCCCC GGATGCTGCC CCTGCCCGCC
    GCCGCCGATG GGGAAAGTAA TTCTGTGATC TGCAACTGTG GCCAGGAGGC CGTGCTGCTC
    ACTGTGAGGA AGGAGGGTCC CAACCAGGGC CGGCAGTTCT ATAAGTGTGG TGGCGGTGGC
    TGTGACTTCT TCCTGTGGGC TGATAGCAGC GCCCTGGAAC CCAGGGGGCC TCCTGCCTCT
    GCACTGGGGC CTGCGGGCGG CTCGCTGGGC CACCCACCTG CTATGGGGGA CCGCCGGGGT
    GGGTTTGGCA GACCCGGAGA TGATGGTGGC CGGGGCATGG CCTGCCTGTG TAGCCAGCCC
    GCCGTCACCC GGACTGTGCA GAAGGATGGG CCCAACAAGG GCCGCCAGTT CCACACGTGC
    GCCAAGCCGA GAGAGCAGCA GTGCGGCTTC TTTCAATGGG TGGACGAGAA TGTGGCCCCA
    GGGACTCTCA GAACCCCAAC CTGGCCAAGA GGCAGAGGAA GAACTCAGGG GCCAGAGGCC
    AGAAGCAAAA GAGCCCGGGC CAGTTCCGCA GACATGGGGT CCACTCCCAA GAAACCCCGG
    AAATGCAGCC TTTGCCACCA GCCTGGCCAT ACCCGTCCCT TCTGTCCTCA GAACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.