ROBO3 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_014113325.1	XP_013968800.1	roundabout homolog 3 isoform X1
XM_014113328.1	XP_013968803.1	roundabout homolog 3 isoform X4
XM_014113328.2	XP_013968803.1	roundabout homolog 3 isoform X2
XM_014113326.1	XP_013968801.1	roundabout homolog 3 isoform X2
XM_022418152.1	XP_022273860.1	roundabout homolog 3 isoform X4
XM_014113327.1	XP_013968802.1	roundabout homolog 3 isoform X3
XM_014113329.1	XP_013968804.1	roundabout homolog 3 isoform X5
XM_014113330.1	XP_013968805.1	roundabout homolog 3 isoform X6
XM_014113331.1	XP_013968806.1	roundabout homolog 3 isoform X7
XM_022418151.1	XP_022273859.1	roundabout homolog 3 isoform X1
XM_014113329.2	XP_013968804.1	roundabout homolog 3 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe287892	XM_022418151.1 Latest version!	Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCe287893	XM_022418152.1 Latest version!	Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCe36279	XM_014113325.1 Latest version!	Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCe36280	XM_014113326.1 Latest version!	Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCe36281	XM_014113327.1 Latest version!	Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCe36282	XM_014113328.2 Latest version!	Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCe36283	XM_014113329.2 Latest version!	Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCe36284	XM_014113330.1 Latest version!	Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCe36285	XM_014113331.1 Latest version!	Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
View Old Accession Versions >>
OCe36282	XM_014113328.1	Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCe36283	XM_014113329.1	Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
Hide Old Accession Versions >>

ROBO3 ( XM_022418151.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273859.1 Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe287892
Clone ID Related Accession (Same CDS sequence)	XM_022418151.1
Accession Version	XM_022418151.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	roundabout homolog 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGCT ACCTGCTGAA GACACTGCTG CAGATGAACT TGTTCGCGGA CTCCCTGGCG 
GGGGACATCT CCAACTCCAG CGACCTGCTC TTCGGCTTCA ACTCCTCGGT GGCGGCGCTC 
AACCACAGCC TGCTGCCCCC CGGCGACCCC TCTTTCAACG CGTCAAGGGT CGAGCCGGAG 
GACGCGATGC CGCGCATCGT GGAGCAGCCG CCAGATCTGC TGGTGTCCCG GGGCGAGCCG 
GCCACGCTGC CCTGCCGCGC CGAGGGCCGG CCCCGGCCCA ACATCGAGTG GTACAAGAAC 
GGGGCGCGGG TGGCCACTGC GCGCGAGGAC CCGCGCGCGC ACCGCCTGCT GCTGCCCAGC 
GGCGCCCTCT TCTTCCCCCG CATCGTGCAC GGGCGCCGCG CGCGCCCCGA CGAGGGTGTC 
TACACTTGCG TGGCGCGCAA CTACCTGGGG GCAGCGGCCA GCAGAAATGC CTCGCTGGAA 
GTGGCGGTCC TCCGTGATGA TTTCCGGCAG TCTCCTGGAA ACGTGGTGGT GGCAGTGGGC 
GAGCCAGCGG TGATGGAATG CGTTCCCCCC CGTGGCCACC CGGAGCCTTC CGTGTCCTGG 
AAGAAGGACA GTGCAAGACT CAGAGAGGAG GAGGGAAGGA TCACGATTCG TGGAGGGAAG 
CTGATGATGT CACATACACT CAAGAGTGAT GCGGGGATGT ATGTGTGCGT GGCCTCCAAC 
ATGGCAGGAG AACGGGAGAG TGGGGCAGCT GAACTTGTGG TACTGGAGCG ACCCTCATTC 
CTGCGTAGAC CAGTCAACCA GGTGGTCCTG GCAGATGCCC CCGTGGATTT CCCATGTGAG 
GTGCAGGGAG ATCCCCCACC CCGTCTACGC TGGCGCAAGG AGGATGGGGA ACTGCCCACC 
GGCAGGTATG AGATCCGGAG TGACCACAGC CTTTGGATCG GCCGCGTGAC TGCCGCCGAC 
GAGGGGACCT ACACCTGTGT GGCTGAGAAC AGCGTGGGCC GCGCCGAAGC ATCTGGCTCC 
CTCAGTGTTC ACGTCCCACC CCAGCTGGTG ACCCAGCCGC AGGACCAGAT GGCAGCTCCT 
GGAGAGAGTG TGGCTTTCCA ATGCGAGACC AAAGGAAACC CCCCACCTGC CATCTTCTGG 
CAGAAGGAGG GAAGTCAGGT TCTACTTTTC CCCAGCCAGC CGCTTCAGCC CAAGGGGCGC 
TTCTCTGTGT CTCCCAGAGG CCAGCTCAAC ATTACGGATG TGCAGAGCGG GGATGCTGGC 
TACTACGTGT GTCAGGCCGT TAGTGTGGCC GGGAGCGTCC TGGCTAAGGC CCTGCTGGAG 
GTAAAAAGAG CCTCCTCCTT GGATGGGCTG CCTCCTATCA TCCTCCAGGG ACCAGCCAAT 
CAGACGCTGG CACTTGGCTC CCCTGTGTGG CTGCCATGCA GAGTGACCGG GAACCCTCAA 
CCGAGTGTCC GATGGAAGAA GGATGGGCAG TGGCTGCAGG GGGACGACCT CCAGCTCAAC 
CTAATGGCCA ATGGTACTCT GTACATCGCC AGCGTGCAGG AGATGGACCT GGGTTTCTAC 
AGCTGTGTGG CCAAGAGTTC CATGGGGGAG GCCACATGGA GCAGCTGGCT TAGGAGGCGG 
GAAGATTGGG GAGTATCACC AGAGCCCCCT ACAGAACCCA GTACCCCTCC AGGGCCTCCC 
TCTCAGCCAG TGGTCACTGA GATCACCAAG AACAGCATTA CCCTGACCTG GAAGCCCAAC 
CCGCAGGCGG GGGCCACAGT CACCTCTTAT GTGATAGAGG CCTTCAGCCA AGCAGCTGGC 
AACACGTGGC GGACAGTGGC AGATGGTGTG CAGCTGGAGA CACACACGGT CAGTGGTCTG 
CAGCCCAACA CCATCTATCT GTTTCTGGTG CGAGCTGTGG GAGCCTGGGG CCTCAGTGAG 
CCCAGCCCCG TCTCTGAGCC CATCCGCACC CAGGACAGCA ACCCATCCAG GCCAGTGGAG 
GACCCATGGA GAGGCCAGCG GGGACTGGCT GAAGTGGCTG TGCATGTGCA GGAGCCCGTA 
GTCCTTGGGC TCCGGACCTT GCAGGTGTCC TGGACTGTAG ATGGCCCAGT CCAGCTGGTG 
CAAGGTTTCC GGGTGTCTTG GAGGGTAGCA GGTCCTGATG GGGGAAGCTG GACAGTACTG 
GACCTACAGT CCCCAAGCCA GCAAAGTACT GTGCTAAGAG GACTCCCCCC AGGGACCCAA 
ATTCAGATCA AAGTGCAAGC CCAAGGCCAG GAGGGGCTGG GGCCTGAAAG TCCCTTTGTG 
ACCAGGAGCA TTCCGGAGGA AGCCCCCAGT GGCCCCCCCC AGGGAGTGAC AGTGGCCTTG 
GGGGGTGAAG GCAACAGCAG TGTCACGGTG TCCTGGGAAC CTCCCCTCCC CTCCCAGCAA 
AATGGGGTGA TCAAGGAATA CCAGATCTGG TGCCTGGGCA ATGAGAGCCG CTTCCACCTC 
AACCGGTCTG CGGCAGGCTG GGCACGCTCC GCCGTGCTCC GGGGACTGCT GCCCGGCCTT 
CTCTACCGGA CCCGGGTCGC GGCGGCCACC AGCGCCGGCG TGGGCGTGGC CAGCGTCCCG 
GTGTCGGTGC AGCTGCCCTC CCCGCCGGAG TCGGAGCCCG GGCTCGAGGT GCGCCCGGGG 
CTGGCGGAGC GCCTGGCCAG GGTGCTGCGG GCGCCTGCCT TCCTGGCGGG CAGCGGGGCC 
GCCTGCGGGG CGCTGCTGCT CGCGCTCTGC GCCGTCCTCT ACCGGCGCCG GAGGCAGCGC 
AAGGAGCTCA GTCACTACAC GGCCTCCTTT GCCTACACAC CGGCAGTGTC CTTCCCACAC 
TCAGAGGGCC TCTCTGGAGC CAGTTCCAGG CCACCCATGG GCCTCGGCCC CGCCCCCTAC 
CCTTGGCTGG CCGACTCGTG GCCCCACCCA TCTCGAAGCC CCTCAGCCCC GGAGCCCAGG 
GGGAGCTGCT GCCCCAGCAA TCCTGACCCA GACGACAGAT ATTACAATGA AGCAGGGATC 
TCCCTGTACC TGGCTCAGAC GGCCCGAGGC ACTGCCGCCC CTACAGAGGG TCCTGTCTAC 
AGCACCATTG ACCCAGCTGG GGAGGAGCTG CAGACCTTCC ACGGGGGGTT CCCCCCAAAT 
CCCTCGGGGG ACCCAGGCGC CTGGAGCCAA TATGCTCCTC CAGAATGGAG CCAGGGGGAC 
AGTGGAGCCA GGGGAGGCAA AGTAAAGCTT CTGGGAAAAG CTGTGCAGAT GCCCTCTCTC 
AACTGGCCGG AAGCCCTGCC ACCACCTCCC CCTTCCTGTG AACTGAGCTG CCTAGAGGGG 
CCTGAGGAAG ATCTGGAAGG CAGCTCAGAG CCAGAAGAGT GGTGCCCACC AATGTCTGAG 
AGGAGCCATC TGACAGAGCC CAGCTCCACT GGAGGGTGCT TGGTCCCTCC ATCCCAAGGG 
GAAGCCCCCT CTCCCACATC TTCCTATGGA CAGCAGTCCA CAGCTACTCT TACCCCCTCA 
CCTCCTGACC CTCCCCAGCC CCCCACTGAC ATCCCCCATC TCCACCAGAT GCCCAGGAGG 
GTGCCCCTGG GGCCAAGTTC CCCTCTCAGT GTATCCCAGC CCACTCTGAG TAGCCATGAA 
GGGAGGCCTG CTGGCCTGGG TGCTGGCCCC ACAGCCTCCC ATCACCTCAA CCCCAGTCCT 
GTCCCTAGTA CAGCCAGCAG TGCCCCAGGG AGAACCCGGC AGGTGCCTGG GGAGATGACC 
CCTCCGCTTC AAGGGCCCCG GGCCCGAATC CGGAAGCCCA AGGCTGTTCC CTACCGGCGG 
GAGCACAGTC CTGGCGGTCT CTCCCCAGAT TTGCCCCCAC CACCCTTGCC GCCACCAGAG 
GAAGAGACGA GCTGGGCCTT AGGGCTGAGA GCAGCAGGCA GCATGTCCTC CCTGGAACGA 
GAGCGGGAGC GCAGTGGGGA GAGAATGGTG CAGGCTGGGC TCCTGGGGGC CCAGCGGGGT 
CCCCACCTGG ATGAAGAGGC CTGGCTCCCT TACAGCCGAC CGAGCTTCCT GTCCCGGGGC 
CAGGGGCCCA ACACCTGTTC CACAGCCGGC AGCAACTCTT CGAGAGGCTC TGGCAGCTCT 
CGAGGCTCCC GGGGACCTGG ACGGAGCCGG AGCCGGAGCC GGAGCCAGAG CCAGAGGCCA 
GGACAGAAAT GTGGAGAGGT GCGGGCTAGG GAACCAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273859.1
CDS	98..4279
Translation	MLRYLLKTLLQMNLFADSLAGDISNSSDLLFGFNSSVAALNHSLLPPGDPSFNASRVEPEDAMPRIVEQPPDLLVSRGEPATLPCRAEGRPRPNIEWYKNGARVATAREDPRAHRLLLPSGALFFPRIVHGRRARPDEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVMECVPPRGHPEPSVSWKKDSARLREEEGRITIRGGKLMMSHTLKSDAGMYVCVASNMAGERESGAAELVVLERPSFLRRPVNQVVLADAPVDFPCEVQGDPPPRLRWRKEDGELPTGRYEIRSDHSLWIGRVTAADEGTYTCVAENSVGRAEASGSLSVHVPPQLVTQPQDQMAAPGESVAFQCETKGNPPPAIFWQKEGSQVLLFPSQPLQPKGRFSVSPRGQLNITDVQSGDAGYYVCQAVSVAGSVLAKALLEVKRASSLDGLPPIILQGPANQTLALGSPVWLPCRVTGNPQPSVRWKKDGQWLQGDDLQLNLMANGTLYIASVQEMDLGFYSCVAKSSMGEATWSSWLRRREDWGVSPEPPTEPSTPPGPPSQPVVTEITKNSITLTWKPNPQAGATVTSYVIEAFSQAAGNTWRTVADGVQLETHTVSGLQPNTIYLFLVRAVGAWGLSEPSPVSEPIRTQDSNPSRPVEDPWRGQRGLAEVAVHVQEPVVLGLRTLQVSWTVDGPVQLVQGFRVSWRVAGPDGGSWTVLDLQSPSQQSTVLRGLPPGTQIQIKVQAQGQEGLGPESPFVTRSIPEEAPSGPPQGVTVALGGEGNSSVTVSWEPPLPSQQNGVIKEYQIWCLGNESRFHLNRSAAGWARSAVLRGLLPGLLYRTRVAAATSAGVGVASVPVSVQLPSPPESEPGLEVRPGLAERLARVLRAPAFLAGSGAACGALLLALCAVLYRRRRQRKELSHYTASFAYTPAVSFPHSEGLSGASSRPPMGLGPAPYPWLADSWPHPSRSPSAPEPRGSCCPSNPDPDDRYYNEAGISLYLAQTARGTAAPTEGPVYSTIDPAGEELQTFHGGFPPNPSGDPGAWSQYAPPEWSQGDSGARGGKVKLLGKAVQMPSLNWPEALPPPPPSCELSCLEGPEEDLEGSSEPEEWCPPMSERSHLTEPSSTGGCLVPPSQGEAPSPTSSYGQQSTATLTPSPPDPPQPPTDIPHLHQMPRRVPLGPSSPLSVSQPTLSSHEGRPAGLGAGPTASHHLNPSPVPSTASSAPGRTRQVPGEMTPPLQGPRARIRKPKAVPYRREHSPGGLSPDLPPPPLPPPEEETSWALGLRAAGSMSSLERERERSGERMVQAGLLGAQRGPHLDEEAWLPYSRPSFLSRGQGPNTCSTAGSNSSRGSGSSRGSRGPGRSRSRSRSQSQRPGQKCGEVRAREPR

Target ORF information:

RefSeq Version	XM_022418151.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022418151.1

ORF Insert Sequence:

ATGCTGCGCT ACCTGCTGAA GACACTGCTG CAGATGAACT TGTTCGCGGA CTCCCTGGCG 
GGGGACATCT CCAACTCCAG CGACCTGCTC TTCGGCTTCA ACTCCTCGGT GGCGGCGCTC 
AACCACAGCC TGCTGCCCCC CGGCGACCCC TCTTTCAACG CGTCAAGGGT CGAGCCGGAG 
GACGCGATGC CGCGCATCGT GGAGCAGCCG CCAGATCTGC TGGTGTCCCG GGGCGAGCCG 
GCCACGCTGC CCTGCCGCGC CGAGGGCCGG CCCCGGCCCA ACATCGAGTG GTACAAGAAC 
GGGGCGCGGG TGGCCACTGC GCGCGAGGAC CCGCGCGCGC ACCGCCTGCT GCTGCCCAGC 
GGCGCCCTCT TCTTCCCCCG CATCGTGCAC GGGCGCCGCG CGCGCCCCGA CGAGGGTGTC 
TACACTTGCG TGGCGCGCAA CTACCTGGGG GCAGCGGCCA GCAGAAATGC CTCGCTGGAA 
GTGGCGGTCC TCCGTGATGA TTTCCGGCAG TCTCCTGGAA ACGTGGTGGT GGCAGTGGGC 
GAGCCAGCGG TGATGGAATG CGTTCCCCCC CGTGGCCACC CGGAGCCTTC CGTGTCCTGG 
AAGAAGGACA GTGCAAGACT CAGAGAGGAG GAGGGAAGGA TCACGATTCG TGGAGGGAAG 
CTGATGATGT CACATACACT CAAGAGTGAT GCGGGGATGT ATGTGTGCGT GGCCTCCAAC 
ATGGCAGGAG AACGGGAGAG TGGGGCAGCT GAACTTGTGG TACTGGAGCG ACCCTCATTC 
CTGCGTAGAC CAGTCAACCA GGTGGTCCTG GCAGATGCCC CCGTGGATTT CCCATGTGAG 
GTGCAGGGAG ATCCCCCACC CCGTCTACGC TGGCGCAAGG AGGATGGGGA ACTGCCCACC 
GGCAGGTATG AGATCCGGAG TGACCACAGC CTTTGGATCG GCCGCGTGAC TGCCGCCGAC 
GAGGGGACCT ACACCTGTGT GGCTGAGAAC AGCGTGGGCC GCGCCGAAGC ATCTGGCTCC 
CTCAGTGTTC ACGTCCCACC CCAGCTGGTG ACCCAGCCGC AGGACCAGAT GGCAGCTCCT 
GGAGAGAGTG TGGCTTTCCA ATGCGAGACC AAAGGAAACC CCCCACCTGC CATCTTCTGG 
CAGAAGGAGG GAAGTCAGGT TCTACTTTTC CCCAGCCAGC CGCTTCAGCC CAAGGGGCGC 
TTCTCTGTGT CTCCCAGAGG CCAGCTCAAC ATTACGGATG TGCAGAGCGG GGATGCTGGC 
TACTACGTGT GTCAGGCCGT TAGTGTGGCC GGGAGCGTCC TGGCTAAGGC CCTGCTGGAG 
GTAAAAAGAG CCTCCTCCTT GGATGGGCTG CCTCCTATCA TCCTCCAGGG ACCAGCCAAT 
CAGACGCTGG CACTTGGCTC CCCTGTGTGG CTGCCATGCA GAGTGACCGG GAACCCTCAA 
CCGAGTGTCC GATGGAAGAA GGATGGGCAG TGGCTGCAGG GGGACGACCT CCAGCTCAAC 
CTAATGGCCA ATGGTACTCT GTACATCGCC AGCGTGCAGG AGATGGACCT GGGTTTCTAC 
AGCTGTGTGG CCAAGAGTTC CATGGGGGAG GCCACATGGA GCAGCTGGCT TAGGAGGCGG 
GAAGATTGGG GAGTATCACC AGAGCCCCCT ACAGAACCCA GTACCCCTCC AGGGCCTCCC 
TCTCAGCCAG TGGTCACTGA GATCACCAAG AACAGCATTA CCCTGACCTG GAAGCCCAAC 
CCGCAGGCGG GGGCCACAGT CACCTCTTAT GTGATAGAGG CCTTCAGCCA AGCAGCTGGC 
AACACGTGGC GGACAGTGGC AGATGGTGTG CAGCTGGAGA CACACACGGT CAGTGGTCTG 
CAGCCCAACA CCATCTATCT GTTTCTGGTG CGAGCTGTGG GAGCCTGGGG CCTCAGTGAG 
CCCAGCCCCG TCTCTGAGCC CATCCGCACC CAGGACAGCA ACCCATCCAG GCCAGTGGAG 
GACCCATGGA GAGGCCAGCG GGGACTGGCT GAAGTGGCTG TGCATGTGCA GGAGCCCGTA 
GTCCTTGGGC TCCGGACCTT GCAGGTGTCC TGGACTGTAG ATGGCCCAGT CCAGCTGGTG 
CAAGGTTTCC GGGTGTCTTG GAGGGTAGCA GGTCCTGATG GGGGAAGCTG GACAGTACTG 
GACCTACAGT CCCCAAGCCA GCAAAGTACT GTGCTAAGAG GACTCCCCCC AGGGACCCAA 
ATTCAGATCA AAGTGCAAGC CCAAGGCCAG GAGGGGCTGG GGCCTGAAAG TCCCTTTGTG 
ACCAGGAGCA TTCCGGAGGA AGCCCCCAGT GGCCCCCCCC AGGGAGTGAC AGTGGCCTTG 
GGGGGTGAAG GCAACAGCAG TGTCACGGTG TCCTGGGAAC CTCCCCTCCC CTCCCAGCAA 
AATGGGGTGA TCAAGGAATA CCAGATCTGG TGCCTGGGCA ATGAGAGCCG CTTCCACCTC 
AACCGGTCTG CGGCAGGCTG GGCACGCTCC GCCGTGCTCC GGGGACTGCT GCCCGGCCTT 
CTCTACCGGA CCCGGGTCGC GGCGGCCACC AGCGCCGGCG TGGGCGTGGC CAGCGTCCCG 
GTGTCGGTGC AGCTGCCCTC CCCGCCGGAG TCGGAGCCCG GGCTCGAGGT GCGCCCGGGG 
CTGGCGGAGC GCCTGGCCAG GGTGCTGCGG GCGCCTGCCT TCCTGGCGGG CAGCGGGGCC 
GCCTGCGGGG CGCTGCTGCT CGCGCTCTGC GCCGTCCTCT ACCGGCGCCG GAGGCAGCGC 
AAGGAGCTCA GTCACTACAC GGCCTCCTTT GCCTACACAC CGGCAGTGTC CTTCCCACAC 
TCAGAGGGCC TCTCTGGAGC CAGTTCCAGG CCACCCATGG GCCTCGGCCC CGCCCCCTAC 
CCTTGGCTGG CCGACTCGTG GCCCCACCCA TCTCGAAGCC CCTCAGCCCC GGAGCCCAGG 
GGGAGCTGCT GCCCCAGCAA TCCTGACCCA GACGACAGAT ATTACAATGA AGCAGGGATC 
TCCCTGTACC TGGCTCAGAC GGCCCGAGGC ACTGCCGCCC CTACAGAGGG TCCTGTCTAC 
AGCACCATTG ACCCAGCTGG GGAGGAGCTG CAGACCTTCC ACGGGGGGTT CCCCCCAAAT 
CCCTCGGGGG ACCCAGGCGC CTGGAGCCAA TATGCTCCTC CAGAATGGAG CCAGGGGGAC 
AGTGGAGCCA GGGGAGGCAA AGTAAAGCTT CTGGGAAAAG CTGTGCAGAT GCCCTCTCTC 
AACTGGCCGG AAGCCCTGCC ACCACCTCCC CCTTCCTGTG AACTGAGCTG CCTAGAGGGG 
CCTGAGGAAG ATCTGGAAGG CAGCTCAGAG CCAGAAGAGT GGTGCCCACC AATGTCTGAG 
AGGAGCCATC TGACAGAGCC CAGCTCCACT GGAGGGTGCT TGGTCCCTCC ATCCCAAGGG 
GAAGCCCCCT CTCCCACATC TTCCTATGGA CAGCAGTCCA CAGCTACTCT TACCCCCTCA 
CCTCCTGACC CTCCCCAGCC CCCCACTGAC ATCCCCCATC TCCACCAGAT GCCCAGGAGG 
GTGCCCCTGG GGCCAAGTTC CCCTCTCAGT GTATCCCAGC CCACTCTGAG TAGCCATGAA 
GGGAGGCCTG CTGGCCTGGG TGCTGGCCCC ACAGCCTCCC ATCACCTCAA CCCCAGTCCT 
GTCCCTAGTA CAGCCAGCAG TGCCCCAGGG AGAACCCGGC AGGTGCCTGG GGAGATGACC 
CCTCCGCTTC AAGGGCCCCG GGCCCGAATC CGGAAGCCCA AGGCTGTTCC CTACCGGCGG 
GAGCACAGTC CTGGCGGTCT CTCCCCAGAT TTGCCCCCAC CACCCTTGCC GCCACCAGAG 
GAAGAGACGA GCTGGGCCTT AGGGCTGAGA GCAGCAGGCA GCATGTCCTC CCTGGAACGA 
GAGCGGGAGC GCAGTGGGGA GAGAATGGTG CAGGCTGGGC TCCTGGGGGC CCAGCGGGGT 
CCCCACCTGG ATGAAGAGGC CTGGCTCCCT TACAGCCGAC CGAGCTTCCT GTCCCGGGGC 
CAGGGGCCCA ACACCTGTTC CACAGCCGGC AGCAACTCTT CGAGAGGCTC TGGCAGCTCT 
CGAGGCTCCC GGGGACCTGG ACGGAGCCGG AGCCGGAGCC GGAGCCAGAG CCAGAGGCCA 
GGACAGAAAT GTGGAGAGGT GCGGGCTAGG GAACCAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO3 ( XM_022418152.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273860.1 Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe287893
Clone ID Related Accession (Same CDS sequence)	XM_022418152.1
Accession Version	XM_022418152.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	roundabout homolog 3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGCT ACCTGCTGAA GACACTGCTG CAGATGAACT TGTTCGCGGA CTCCCTGGCG 
GGGGACATCT CCAACTCCAG CGACCTGCTC TTCGGCTTCA ACTCCTCGGT GGCGGCGCTC 
AACCACAGCC TGCTGCCCCC CGGCGACCCC TCTTTCAACG CGTCAAGGGT CGAGCCGGAG 
GACGCGATGC CGCGCATCGT GGAGCAGCCG CCAGATCTGC TGGTGTCCCG GGGCGAGCCG 
GCCACGCTGC CCTGCCGCGC CGAGGGCCGG CCCCGGCCCA ACATCGAGTG GTACAAGAAC 
GGGGCGCGGG TGGCCACTGC GCGCGAGGAC CCGCGCGCGC ACCGCCTGCT GCTGCCCAGC 
GGCGCCCTCT TCTTCCCCCG CATCGTGCAC GGGCGCCGCG CGCGCCCCGA CGAGGGTGTC 
TACACTTGCG TGGCGCGCAA CTACCTGGGG GCAGCGGCCA GCAGAAATGC CTCGCTGGAA 
GTGGCGGTCC TCCGTGATGA TTTCCGGCAG TCTCCTGGAA ACGTGGTGGT GGCAGTGGGC 
GAGCCAGCGG TGATGGAATG CGTTCCCCCC CGTGGCCACC CGGAGCCTTC CGTGTCCTGG 
AAGAAGGACA GTGCAAGACT CAGAGAGGAG GAGGGAAGGA TCACGATTCG TGGAGGGAAG 
CTGATGATGT CACATACACT CAAGAGTGAT GCGGGGATGT ATGTGTGCGT GGCCTCCAAC 
ATGGCAGGAG AACGGGAGAG TGGGGCAGCT GAACTTGTGG TACTGGAGCG ACCCTCATTC 
CTGCGTAGAC CAGTCAACCA GGTGGTCCTG GCAGATGCCC CCGTGGATTT CCCATGTGAG 
GTGCAGGGAG ATCCCCCACC CCGTCTACGC TGGCGCAAGG AGGATGGGGA ACTGCCCACC 
GGCAGGTATG AGATCCGGAG TGACCACAGC CTTTGGATCG GCCGCGTGAC TGCCGCCGAC 
GAGGGGACCT ACACCTGTGT GGCTGAGAAC AGCGTGGGCC GCGCCGAAGC ATCTGGCTCC 
CTCAGTGTTC ACGTCCCACC CCAGCTGGTG ACCCAGCCGC AGGACCAGAT GGCAGCTCCT 
GGAGAGAGTG TGGCTTTCCA ATGCGAGACC AAAGGAAACC CCCCACCTGC CATCTTCTGG 
CAGAAGGAGG GAAGTCAGGT TCTACTTTTC CCCAGCCAGC CGCTTCAGCC CAAGGGGCGC 
TTCTCTGTGT CTCCCAGAGG CCAGCTCAAC ATTACGGATG TGCAGAGCGG GGATGCTGGC 
TACTACGTGT GTCAGGCCGT TAGTGTGGCC GGGAGCGTCC TGGCTAAGGC CCTGCTGGAG 
GTAAAAAGAG CCTCCTCCTT GGATGGGCTG CCTCCTATCA TCCTCCAGGG ACCAGCCAAT 
CAGACGCTGG CACTTGGCTC CCCTGTGTGG CTGCCATGCA GAGTGACCGG GAACCCTCAA 
CCGAGTGTCC GATGGAAGAA GGATGGGCAG TGGCTGCAGG GGGACGACCT CCAGCTCAAC 
CTAATGGCCA ATGGTACTCT GTACATCGCC AGCGTGCAGG AGATGGACCT GGGTTTCTAC 
AGCTGTGTGG CCAAGAGTTC CATGGGGGAG GCCACATGGA GCAGCTGGCT TAGGAGGCGG 
GAAGATTGGG GAGTATCACC AGAGCCCCCT ACAGAACCCA GTACCCCTCC AGGGCCTCCC 
TCTCAGCCAG TGGTCACTGA GATCACCAAG AACAGCATTA CCCTGACCTG GAAGCCCAAC 
CCGCAGGCGG GGGCCACAGT CACCTCTTAT GTGATAGAGG CCTTCAGCCA AGCAGCTGGC 
AACACGTGGC GGACAGTGGC AGATGGTGTG CAGCTGGAGA CACACACGGT CAGTGGTCTG 
CAGCCCAACA CCATCTATCT GTTTCTGGTG CGAGCTGTGG GAGCCTGGGG CCTCAGTGAG 
CCCAGCCCCG TCTCTGAGCC CATCCGCACC CAGGACAGCA ACCCATCCAG GCCAGTGGAG 
GACCCATGGA GAGGCCAGCG GGGACTGGCT GAAGTGGCTG TGCATGTGCA GGAGCCCGTA 
GTCCTTGGGC TCCGGACCTT GCAGGTGTCC TGGACTGTAG ATGGCCCAGT CCAGCTGGTG 
CAAGGTTTCC GGGTGTCTTG GAGGGTAGCA GGTCCTGATG GGGGAAGCTG GACAGTACTG 
GACCTACAGT CCCCAAGCCA GCAAAGTACT GTGCTAAGAG GACTCCCCCC AGGGACCCAA 
ATTCAGATCA AAGTGCAAGC CCAAGGCCAG GAGGGGCTGG GGCCTGAAAG TCCCTTTGTG 
ACCAGGAGCA TTCCGGAGGA AGCCCCCAGT GGCCCCCCCC AGGGAGTGAC AGTGGCCTTG 
GGGGGTGAAG GCAACAGCAG TGTCACGGTG TCCTGGGAAC CTCCCCTCCC CTCCCAGCAA 
AATGGGGTGA TCAAGGAATA CCAGATCTGG TGCCTGGGCA ATGAGAGCCG CTTCCACCTC 
AACCGGTCTG CGGCAGGCTG GGCACGCTCC GCCGTGCTCC GGGGACTGCT GCCCGGCCTT 
CTCTACCGGA CCCGGGTCGC GGCGGCCACC AGCGCCGGCG TGGGCGTGGC CAGCGTCCCG 
GTGTCGGTGC AGCTGCCCTC CCCGCCGGAG TCGGAGCCCG GGCTCGAGGT GCGCCCGGGG 
CTGGCGGAGC GCCTGGCCAG GGTGCTGCGG GCGCCTGCCT TCCTGGCGGG CAGCGGGGCC 
GCCTGCGGGG CGCTGCTGCT CGCGCTCTGC GCCGTCCTCT ACCGGCGCCG GAGGCAGCGC 
AAGGAGCTCA GTCACTACAC GGCCTCCTTT GCCTACACAC CGGCAGTGTC CTTCCCACAC 
TCAGAGGGCC TCTCTGGAGC CAGTTCCAGG CCACCCATGG GCCTCGGCCC CGCCCCCTAC 
CCTTGGCTGG CCGACTCGTG GCCCCACCCA TCTCGAAGCC CCTCAGCCCC GGAGCCCAGG 
GGGAGCTGCT GCCCCAGCAA TCCTGACCCA GACGACAGAT ATTACAATGA AGCAGGGATC 
TCCCTGTACC TGGCTCAGAC GGCCCGAGGC ACTGCCGCCC CTACAGAGGG TCCTGTCTAC 
AGCACCATTG ACCCAGCTGG GGAGGAGCTG CAGACCTTCC ACGGGGGGTT CCCCCCAAAT 
CCCTCGGGGG ACCCAGGCGC CTGGAGCCAA TATGCTCCTC CAGAATGGAG CCAGGGGGAC 
AGTGGAGCCA GGGGAGGCAA AGTAAAGCTT CTGGGAAAAG CTGTGCAGAT GCCCTCTCTC 
AACTGGCCGG AAGCCCTGCC ACCACCTCCC CCTTCCTGTG AACTGAGCTG CCTAGAGGGG 
CCTGAGGAAG ATCTGGAAGG CAGCTCAGAG CCAGAAGAGT GGTGCCCACC AATGTCTGAG 
AGGAGCCATC TGACAGAGCC CAGCTCCACT GGAGGGTGCT TGGTCCCTCC ATCCCAAGGG 
GAAGCCCCCT CTCCCACATC TTCCTATGGA CAGCAGTCCA CAGCTACTCT TACCCCCTCA 
CCTCCTGACC CTCCCCAGCC CCCCACTGAC ATCCCCCATC TCCACCAGAT GCCCAGGAGG 
GTGCCCCTGG GGCCAAGTTC CCCTCTCAGT GTATCCCAGC CCACTCTGAG TAGCCATGAA 
GGGAGGCCTG CTGGCCTGGG TGCTGGCCCC ACAGCCTCCC ATCACCTCAA CCCCAGTCCT 
GTCCCTAGTA CAGCCAGCAG TGCCCCAGGG AGAACCCGGC AGGTGCCTGG GGAGATGACC 
CCTCCGCTTC AAGGGCCCCG GGCCCGAATC CGGAAGCCCA AGGCTGTTCC CTACCGGCGG 
GAGCACAGTC CTGGCGGTCT CTCCCCAGAT TTGCCCCCAC CACCCTTGCC GCCACCAGAG 
GAAGAGACGA GCTGGGCCTT AGGGCTGAGA GCAGCAGGCA GCATGTCCTC CCTGGAACGA 
GAGCGGGAGC GCAGTGGGGA GAGAATGGTG CAGGCTGGGC TCCTGGGGGC CCAGCGGGGT 
CCCCACCTGG ATGAAGAGGC CTGGCTCCCT TACAGCCGAC CGAGCTTCCT GTCCCGGGGC 
CAGGGGCCCA ACACCTGTTC CACAGCCGGC AGCAACTCTT CGAGAGGCTC TGGCAGCTCT 
CGAGGCTCCC GGGGACCTGG ACGGAGCCGG AGCCGGAGCC GGAGCCAGAG CCAGAGGCCA 
GGACAGAAAT GTGGAGAGGA ACCAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273860.1
CDS	96..4265
Translation	MLRYLLKTLLQMNLFADSLAGDISNSSDLLFGFNSSVAALNHSLLPPGDPSFNASRVEPEDAMPRIVEQPPDLLVSRGEPATLPCRAEGRPRPNIEWYKNGARVATAREDPRAHRLLLPSGALFFPRIVHGRRARPDEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVMECVPPRGHPEPSVSWKKDSARLREEEGRITIRGGKLMMSHTLKSDAGMYVCVASNMAGERESGAAELVVLERPSFLRRPVNQVVLADAPVDFPCEVQGDPPPRLRWRKEDGELPTGRYEIRSDHSLWIGRVTAADEGTYTCVAENSVGRAEASGSLSVHVPPQLVTQPQDQMAAPGESVAFQCETKGNPPPAIFWQKEGSQVLLFPSQPLQPKGRFSVSPRGQLNITDVQSGDAGYYVCQAVSVAGSVLAKALLEVKRASSLDGLPPIILQGPANQTLALGSPVWLPCRVTGNPQPSVRWKKDGQWLQGDDLQLNLMANGTLYIASVQEMDLGFYSCVAKSSMGEATWSSWLRRREDWGVSPEPPTEPSTPPGPPSQPVVTEITKNSITLTWKPNPQAGATVTSYVIEAFSQAAGNTWRTVADGVQLETHTVSGLQPNTIYLFLVRAVGAWGLSEPSPVSEPIRTQDSNPSRPVEDPWRGQRGLAEVAVHVQEPVVLGLRTLQVSWTVDGPVQLVQGFRVSWRVAGPDGGSWTVLDLQSPSQQSTVLRGLPPGTQIQIKVQAQGQEGLGPESPFVTRSIPEEAPSGPPQGVTVALGGEGNSSVTVSWEPPLPSQQNGVIKEYQIWCLGNESRFHLNRSAAGWARSAVLRGLLPGLLYRTRVAAATSAGVGVASVPVSVQLPSPPESEPGLEVRPGLAERLARVLRAPAFLAGSGAACGALLLALCAVLYRRRRQRKELSHYTASFAYTPAVSFPHSEGLSGASSRPPMGLGPAPYPWLADSWPHPSRSPSAPEPRGSCCPSNPDPDDRYYNEAGISLYLAQTARGTAAPTEGPVYSTIDPAGEELQTFHGGFPPNPSGDPGAWSQYAPPEWSQGDSGARGGKVKLLGKAVQMPSLNWPEALPPPPPSCELSCLEGPEEDLEGSSEPEEWCPPMSERSHLTEPSSTGGCLVPPSQGEAPSPTSSYGQQSTATLTPSPPDPPQPPTDIPHLHQMPRRVPLGPSSPLSVSQPTLSSHEGRPAGLGAGPTASHHLNPSPVPSTASSAPGRTRQVPGEMTPPLQGPRARIRKPKAVPYRREHSPGGLSPDLPPPPLPPPEEETSWALGLRAAGSMSSLERERERSGERMVQAGLLGAQRGPHLDEEAWLPYSRPSFLSRGQGPNTCSTAGSNSSRGSGSSRGSRGPGRSRSRSRSQSQRPGQKCGEEPR

Target ORF information:

RefSeq Version	XM_022418152.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022418152.1

ORF Insert Sequence:

ATGCTGCGCT ACCTGCTGAA GACACTGCTG CAGATGAACT TGTTCGCGGA CTCCCTGGCG 
GGGGACATCT CCAACTCCAG CGACCTGCTC TTCGGCTTCA ACTCCTCGGT GGCGGCGCTC 
AACCACAGCC TGCTGCCCCC CGGCGACCCC TCTTTCAACG CGTCAAGGGT CGAGCCGGAG 
GACGCGATGC CGCGCATCGT GGAGCAGCCG CCAGATCTGC TGGTGTCCCG GGGCGAGCCG 
GCCACGCTGC CCTGCCGCGC CGAGGGCCGG CCCCGGCCCA ACATCGAGTG GTACAAGAAC 
GGGGCGCGGG TGGCCACTGC GCGCGAGGAC CCGCGCGCGC ACCGCCTGCT GCTGCCCAGC 
GGCGCCCTCT TCTTCCCCCG CATCGTGCAC GGGCGCCGCG CGCGCCCCGA CGAGGGTGTC 
TACACTTGCG TGGCGCGCAA CTACCTGGGG GCAGCGGCCA GCAGAAATGC CTCGCTGGAA 
GTGGCGGTCC TCCGTGATGA TTTCCGGCAG TCTCCTGGAA ACGTGGTGGT GGCAGTGGGC 
GAGCCAGCGG TGATGGAATG CGTTCCCCCC CGTGGCCACC CGGAGCCTTC CGTGTCCTGG 
AAGAAGGACA GTGCAAGACT CAGAGAGGAG GAGGGAAGGA TCACGATTCG TGGAGGGAAG 
CTGATGATGT CACATACACT CAAGAGTGAT GCGGGGATGT ATGTGTGCGT GGCCTCCAAC 
ATGGCAGGAG AACGGGAGAG TGGGGCAGCT GAACTTGTGG TACTGGAGCG ACCCTCATTC 
CTGCGTAGAC CAGTCAACCA GGTGGTCCTG GCAGATGCCC CCGTGGATTT CCCATGTGAG 
GTGCAGGGAG ATCCCCCACC CCGTCTACGC TGGCGCAAGG AGGATGGGGA ACTGCCCACC 
GGCAGGTATG AGATCCGGAG TGACCACAGC CTTTGGATCG GCCGCGTGAC TGCCGCCGAC 
GAGGGGACCT ACACCTGTGT GGCTGAGAAC AGCGTGGGCC GCGCCGAAGC ATCTGGCTCC 
CTCAGTGTTC ACGTCCCACC CCAGCTGGTG ACCCAGCCGC AGGACCAGAT GGCAGCTCCT 
GGAGAGAGTG TGGCTTTCCA ATGCGAGACC AAAGGAAACC CCCCACCTGC CATCTTCTGG 
CAGAAGGAGG GAAGTCAGGT TCTACTTTTC CCCAGCCAGC CGCTTCAGCC CAAGGGGCGC 
TTCTCTGTGT CTCCCAGAGG CCAGCTCAAC ATTACGGATG TGCAGAGCGG GGATGCTGGC 
TACTACGTGT GTCAGGCCGT TAGTGTGGCC GGGAGCGTCC TGGCTAAGGC CCTGCTGGAG 
GTAAAAAGAG CCTCCTCCTT GGATGGGCTG CCTCCTATCA TCCTCCAGGG ACCAGCCAAT 
CAGACGCTGG CACTTGGCTC CCCTGTGTGG CTGCCATGCA GAGTGACCGG GAACCCTCAA 
CCGAGTGTCC GATGGAAGAA GGATGGGCAG TGGCTGCAGG GGGACGACCT CCAGCTCAAC 
CTAATGGCCA ATGGTACTCT GTACATCGCC AGCGTGCAGG AGATGGACCT GGGTTTCTAC 
AGCTGTGTGG CCAAGAGTTC CATGGGGGAG GCCACATGGA GCAGCTGGCT TAGGAGGCGG 
GAAGATTGGG GAGTATCACC AGAGCCCCCT ACAGAACCCA GTACCCCTCC AGGGCCTCCC 
TCTCAGCCAG TGGTCACTGA GATCACCAAG AACAGCATTA CCCTGACCTG GAAGCCCAAC 
CCGCAGGCGG GGGCCACAGT CACCTCTTAT GTGATAGAGG CCTTCAGCCA AGCAGCTGGC 
AACACGTGGC GGACAGTGGC AGATGGTGTG CAGCTGGAGA CACACACGGT CAGTGGTCTG 
CAGCCCAACA CCATCTATCT GTTTCTGGTG CGAGCTGTGG GAGCCTGGGG CCTCAGTGAG 
CCCAGCCCCG TCTCTGAGCC CATCCGCACC CAGGACAGCA ACCCATCCAG GCCAGTGGAG 
GACCCATGGA GAGGCCAGCG GGGACTGGCT GAAGTGGCTG TGCATGTGCA GGAGCCCGTA 
GTCCTTGGGC TCCGGACCTT GCAGGTGTCC TGGACTGTAG ATGGCCCAGT CCAGCTGGTG 
CAAGGTTTCC GGGTGTCTTG GAGGGTAGCA GGTCCTGATG GGGGAAGCTG GACAGTACTG 
GACCTACAGT CCCCAAGCCA GCAAAGTACT GTGCTAAGAG GACTCCCCCC AGGGACCCAA 
ATTCAGATCA AAGTGCAAGC CCAAGGCCAG GAGGGGCTGG GGCCTGAAAG TCCCTTTGTG 
ACCAGGAGCA TTCCGGAGGA AGCCCCCAGT GGCCCCCCCC AGGGAGTGAC AGTGGCCTTG 
GGGGGTGAAG GCAACAGCAG TGTCACGGTG TCCTGGGAAC CTCCCCTCCC CTCCCAGCAA 
AATGGGGTGA TCAAGGAATA CCAGATCTGG TGCCTGGGCA ATGAGAGCCG CTTCCACCTC 
AACCGGTCTG CGGCAGGCTG GGCACGCTCC GCCGTGCTCC GGGGACTGCT GCCCGGCCTT 
CTCTACCGGA CCCGGGTCGC GGCGGCCACC AGCGCCGGCG TGGGCGTGGC CAGCGTCCCG 
GTGTCGGTGC AGCTGCCCTC CCCGCCGGAG TCGGAGCCCG GGCTCGAGGT GCGCCCGGGG 
CTGGCGGAGC GCCTGGCCAG GGTGCTGCGG GCGCCTGCCT TCCTGGCGGG CAGCGGGGCC 
GCCTGCGGGG CGCTGCTGCT CGCGCTCTGC GCCGTCCTCT ACCGGCGCCG GAGGCAGCGC 
AAGGAGCTCA GTCACTACAC GGCCTCCTTT GCCTACACAC CGGCAGTGTC CTTCCCACAC 
TCAGAGGGCC TCTCTGGAGC CAGTTCCAGG CCACCCATGG GCCTCGGCCC CGCCCCCTAC 
CCTTGGCTGG CCGACTCGTG GCCCCACCCA TCTCGAAGCC CCTCAGCCCC GGAGCCCAGG 
GGGAGCTGCT GCCCCAGCAA TCCTGACCCA GACGACAGAT ATTACAATGA AGCAGGGATC 
TCCCTGTACC TGGCTCAGAC GGCCCGAGGC ACTGCCGCCC CTACAGAGGG TCCTGTCTAC 
AGCACCATTG ACCCAGCTGG GGAGGAGCTG CAGACCTTCC ACGGGGGGTT CCCCCCAAAT 
CCCTCGGGGG ACCCAGGCGC CTGGAGCCAA TATGCTCCTC CAGAATGGAG CCAGGGGGAC 
AGTGGAGCCA GGGGAGGCAA AGTAAAGCTT CTGGGAAAAG CTGTGCAGAT GCCCTCTCTC 
AACTGGCCGG AAGCCCTGCC ACCACCTCCC CCTTCCTGTG AACTGAGCTG CCTAGAGGGG 
CCTGAGGAAG ATCTGGAAGG CAGCTCAGAG CCAGAAGAGT GGTGCCCACC AATGTCTGAG 
AGGAGCCATC TGACAGAGCC CAGCTCCACT GGAGGGTGCT TGGTCCCTCC ATCCCAAGGG 
GAAGCCCCCT CTCCCACATC TTCCTATGGA CAGCAGTCCA CAGCTACTCT TACCCCCTCA 
CCTCCTGACC CTCCCCAGCC CCCCACTGAC ATCCCCCATC TCCACCAGAT GCCCAGGAGG 
GTGCCCCTGG GGCCAAGTTC CCCTCTCAGT GTATCCCAGC CCACTCTGAG TAGCCATGAA 
GGGAGGCCTG CTGGCCTGGG TGCTGGCCCC ACAGCCTCCC ATCACCTCAA CCCCAGTCCT 
GTCCCTAGTA CAGCCAGCAG TGCCCCAGGG AGAACCCGGC AGGTGCCTGG GGAGATGACC 
CCTCCGCTTC AAGGGCCCCG GGCCCGAATC CGGAAGCCCA AGGCTGTTCC CTACCGGCGG 
GAGCACAGTC CTGGCGGTCT CTCCCCAGAT TTGCCCCCAC CACCCTTGCC GCCACCAGAG 
GAAGAGACGA GCTGGGCCTT AGGGCTGAGA GCAGCAGGCA GCATGTCCTC CCTGGAACGA 
GAGCGGGAGC GCAGTGGGGA GAGAATGGTG CAGGCTGGGC TCCTGGGGGC CCAGCGGGGT 
CCCCACCTGG ATGAAGAGGC CTGGCTCCCT TACAGCCGAC CGAGCTTCCT GTCCCGGGGC 
CAGGGGCCCA ACACCTGTTC CACAGCCGGC AGCAACTCTT CGAGAGGCTC TGGCAGCTCT 
CGAGGCTCCC GGGGACCTGG ACGGAGCCGG AGCCGGAGCC GGAGCCAGAG CCAGAGGCCA 
GGACAGAAAT GTGGAGAGGA ACCAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO3 ( XM_014113325.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968800.1 Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X1, mRNA.

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CloneID	OCe36279
Clone ID Related Accession (Same CDS sequence)	XM_014113325.1
Accession Version	XM_014113325.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	roundabout homolog 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGCT ACCTGCTGAA GACACTGCTG CAGATGAACT TGTTCGCGGA CTCCCTGGCG 
GGGGACATCT CCAACTCCAG CGACCTGCTC TTCGGCTTCA ACTCCTCGGT GGCGGCGCTC 
AACCACAGCC TGCTGCCCCC CGGCGACCCC TCTTTCAACG CGTCAAGGGT CGAGCCGGAG 
GACGCGATGC CGCGCATCGT GGAGCAGCCG CCAGATCTGC TGGTGTCCCG GGGCGAGCCG 
GCCACGCTGC CCTGCCGCGC CGAGGGCCGG CCCCGGCCCA ACATCGAGTG GTACAAGAAC 
GGGGCGCGGG TGGCCACTGC GCGCGAGGAC CCGCGCGCGC ACCGCCTGCT GCTGCCCAGC 
GGCGCCCTCT TCTTCCCCCG CATCGTGCAC GGGCGCCGCG CGCGCCCCGA CGAGGGTGTC 
TACACTTGCG TGGCGCGCAA CTACCTGGGG GCAGCGGCCA GCAGAAATGC CTCGCTGGAA 
GTGGCGGTCC TCCGTGATGA TTTCCGGCAG TCTCCTGGAA ACGTGGTGGT GGCAGTGGGC 
GAGCCAGCGG TGATGGAATG CGTTCCCCCC CGTGGCCACC CGGAGCCTTC CGTGTCCTGG 
AAGAAGGACA GTGCAAGACT CAGAGAGGAG GAGGGAAGGA TCACGATTCG TGGAGGGAAG 
CTGATGATGT CACATACACT CAAGAGTGAT GCGGGGATGT ATGTGTGCGT GGCCTCCAAC 
ATGGCAGGAG AACGGGAGAG TGGGGCAGCT GAACTTGTGG TACTGGAGCG ACCCTCATTC 
CTGCGTAGAC CAGTCAACCA GGTGGTCCTG GCAGATGCCC CCGTGGATTT CCCATGTGAG 
GTGCAGGGAG ATCCCCCACC CCGTCTACGC TGGCGCAAGG AGGATGGGGA ACTGCCCACC 
GGCAGGTATG AGATCCGGAG TGACCACAGC CTTTGGATCG GCCGCGTGAC TGCCGCCGAC 
GAGGGGACCT ACACCTGTGT GGCTGAGAAC AGCGTGGGCC GCGCCGAAGC ATCTGGCTCC 
CTCAGTGTTC ACGTCCCACC CCAGCTGGTG ACCCAGCCGC AGGACCAGAT GGCAGCTCCT 
GGAGAGAGTG TGGCTTTCCA ATGCGAGACC AAAGGAAACC CCCCACCTGC CATCTTCTGG 
CAGAAGGAGG GAAGTCAGGT TCTACTTTTC CCCAGCCAGC CGCTTCAGCC CAAGGGGCGC 
TTCTCTGTGT CTCCCAGAGG CCAGCTCAAC ATTACGGATG TGCAGAGCGG GGATGCTGGC 
TACTACGTGT GTCAGGCCGT TAGTGTGGCC GGGAGCGTCC TGGCTAAGGC CCTGCTGGAG 
GTAAAAAGAG CCTCCTCCTT GGATGGGCTG CCTCCTATCA TCCTCCAGGG ACCAGCCAAT 
CAGACGCTGG CACTTGGCTC CCCTGTGTGG CTGCCATGCA GAGTGACCGG GAACCCTCAA 
CCGAGTGTCC GATGGAAGAA GGATGGGCAG TGGCTGCAGG GGGACGACCT CCAGCTCAAC 
CTAATGGCCA ATGGTACTCT GTACATCGCC AGCGTGCAGG AGATGGACCT GGGTTTCTAC 
AGCTGTGTGG CCAAGAGTTC CATGGGGGAG GCCACATGGA GCAGCTGGCT TAGGAGGCGG 
GATTATCTCC TCGCTGCAGA AGATTGGGGA GTATCACCAG AGCCCCCTAC AGAACCCAGT 
ACCCCTCCAG GGCCTCCCTC TCAGCCAGTG GTCACTGAGA TCACCAAGAA CAGCATTACC 
CTGACCTGGA AGCCCAACCC GCAGGCGGGG GCCACAGTCA CCTCTTATGT GATAGAGGCC 
TTCAGCCAAG CAGCTGGCAA CACGTGGCGG ACAGTGGCAG ATGGTGTGCA GCTGGAGACA 
CACACGGTCA GTGGTCTGCA GCCCAACACC ATCTATCTGT TTCTGGTGCG AGCTGTGGGA 
GCCTGGGGCC TCAGTGAGCC CAGCCCCGTC TCTGAGCCCA TCCGCACCCA GGACAGCAAC 
CCATCCAGGC CAGTGGAGGA CCCATGGAGA GGCCAGCGGG GACTGGCTGA AGTGGCTGTG 
CATGTGCAGG AGCCCGTAGT CCTTGGGCTC CGGACCTTGC AGGTGTCCTG GACTGTAGAT 
GGCCCAGTCC AGCTGGTGCA AGGTTTCCGG GTGTCTTGGA GGGTAGCAGG TCCTGATGGG 
GGAAGCTGGA CAGTACTGGA CCTACAGTCC CCAAGCCAGC AAAGTACTGT GCTAAGAGGA 
CTCCCCCCAG GGACCCAAAT TCAGATCAAA GTGCAAGCCC AAGGCCAGGA GGGGCTGGGG 
CCTGAAAGTC CCTTTGTGAC CAGGAGCATT CCGGAGGAAG CCCCCAGTGG CCCCCCCCAG 
GGAGTGACAG TGGCCTTGGG GGGTGAAGGC AACAGCAGTG TCACGGTGTC CTGGGAACCT 
CCCCTCCCCT CCCAGCAAAA TGGGGTGATC AAGGAATACC AGATCTGGTG CCTGGGCAAT 
GAGAGCCGCT TCCACCTCAA CCGGTCTGCG GCAGGCTGGG CACGCTCCGC CGTGCTCCGG 
GGACTGCTGC CCGGCCTTCT CTACCGGACC CGGGTCGCGG CGGCCACCAG CGCCGGCGTG 
GGCGTGGCCA GCGTCCCGGT GTCGGTGCAG CTGCCCTCCC CGCCGGAGTC GGAGCCCGGG 
CTCGAGGTGC GCCCGGGGCT GGCGGAGCGC CTGGCCAGGG TGCTGCGGGC GCCTGCCTTC 
CTGGCGGGCA GCGGGGCCGC CTGCGGGGCG CTGCTGCTCG CGCTCTGCGC CGTCCTCTAC 
CGGCGCCGGA GGCAGCGCAA GGAGCTCAGT CACTACACGG CCTCCTTTGC CTACACACCG 
GCAGTGTCCT TCCCACACTC AGAGGGCCTC TCTGGAGCCA GTTCCAGGCC ACCCATGGGC 
CTCGGCCCCG CCCCCTACCC TTGGCTGGCC GACTCGTGGC CCCACCCATC TCGAAGCCCC 
TCAGCCCCGG AGCCCAGGGG GAGCTGCTGC CCCAGCAATC CTGACCCAGA CGACAGATAT 
TACAATGAAG CAGGGATCTC CCTGTACCTG GCTCAGACGG CCCGAGGCAC TGCCGCCCCT 
ACAGAGGGTC CTGTCTACAG CACCATTGAC CCAGCTGGGG AGGAGCTGCA GACCTTCCAC 
GGGGGGTTCC CCCCAAATCC CTCGGGGGAC CCAGGCGCCT GGAGCCAATA TGCTCCTCCA 
GAATGGAGCC AGGGGGACAG TGGAGCCAGG GGAGGCAAAG TAAAGCTTCT GGGAAAAGCT 
GTGCAGATGC CCTCTCTCAA CTGGCCGGAA GCCCTGCCAC CACCTCCCCC TTCCTGTGAA 
CTGAGCTGCC TAGAGGGGCC TGAGGAAGAT CTGGAAGGCA GCTCAGAGCC AGAAGAGTGG 
TGCCCACCAA TGTCTGAGAG GAGCCATCTG ACAGAGCCCA GCTCCACTGG AGGGTGCTTG 
GTCCCTCCAT CCCAAGGGGA AGCCCCCTCT CCCACATCTT CCTATGGACA GCAGTCCACA 
GCTACTCTTA CCCCCTCACC TCCTGACCCT CCCCAGCCCC CCACTGACAT CCCCCATCTC 
CACCAGATGC CCAGGAGGGT GCCCCTGGGG CCAAGTTCCC CTCTCAGTGT ATCCCAGCCC 
ACTCTGAGTA GCCATGAAGG GAGGCCTGCT GGCCTGGGTG CTGGCCCCAC AGCCTCCCAT 
CACCTCAACC CCAGTCCTGT CCCTAGTACA GCCAGCAGTG CCCCAGGGAG AACCCGGCAG 
GTGCCTGGGG AGATGACCCC TCCGCTTCAA GGGCCCCGGG CCCGAATCCG GAAGCCCAAG 
GCTGTTCCCT ACCGGCGGGA GCACAGTCCT GGCGGTCTCT CCCCAGATTT GCCCCCACCA 
CCCTTGCCGC CACCAGAGGA AGAGACGAGC TGGGCCTTAG GGCTGAGAGC AGCAGGCAGC 
ATGTCCTCCC TGGAACGAGA GCGGGAGCGC AGTGGGGAGA GAATGGTGCA GGCTGGGCTC 
CTGGGGGCCC AGCGGGGTCC CCACCTGGAT GAAGAGGCCT GGCTCCCTTA CAGCCGACCG 
AGCTTCCTGT CCCGGGGCCA GGGGCCCAAC ACCTGTTCCA CAGCCGGCAG CAACTCTTCG 
AGAGGCTCTG GCAGCTCTCG AGGCTCCCGG GGACCTGGAC GGAGCCGGAG CCGGAGCCGG 
AGCCAGAGCC AGAGGCCAGG ACAGAAATGT GGAGAGGTGC GGGCTAGGGA ACCAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968800.1
CDS	196..4395
Translation	MLRYLLKTLLQMNLFADSLAGDISNSSDLLFGFNSSVAALNHSLLPPGDPSFNASRVEPEDAMPRIVEQPPDLLVSRGEPATLPCRAEGRPRPNIEWYKNGARVATAREDPRAHRLLLPSGALFFPRIVHGRRARPDEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVMECVPPRGHPEPSVSWKKDSARLREEEGRITIRGGKLMMSHTLKSDAGMYVCVASNMAGERESGAAELVVLERPSFLRRPVNQVVLADAPVDFPCEVQGDPPPRLRWRKEDGELPTGRYEIRSDHSLWIGRVTAADEGTYTCVAENSVGRAEASGSLSVHVPPQLVTQPQDQMAAPGESVAFQCETKGNPPPAIFWQKEGSQVLLFPSQPLQPKGRFSVSPRGQLNITDVQSGDAGYYVCQAVSVAGSVLAKALLEVKRASSLDGLPPIILQGPANQTLALGSPVWLPCRVTGNPQPSVRWKKDGQWLQGDDLQLNLMANGTLYIASVQEMDLGFYSCVAKSSMGEATWSSWLRRRDYLLAAEDWGVSPEPPTEPSTPPGPPSQPVVTEITKNSITLTWKPNPQAGATVTSYVIEAFSQAAGNTWRTVADGVQLETHTVSGLQPNTIYLFLVRAVGAWGLSEPSPVSEPIRTQDSNPSRPVEDPWRGQRGLAEVAVHVQEPVVLGLRTLQVSWTVDGPVQLVQGFRVSWRVAGPDGGSWTVLDLQSPSQQSTVLRGLPPGTQIQIKVQAQGQEGLGPESPFVTRSIPEEAPSGPPQGVTVALGGEGNSSVTVSWEPPLPSQQNGVIKEYQIWCLGNESRFHLNRSAAGWARSAVLRGLLPGLLYRTRVAAATSAGVGVASVPVSVQLPSPPESEPGLEVRPGLAERLARVLRAPAFLAGSGAACGALLLALCAVLYRRRRQRKELSHYTASFAYTPAVSFPHSEGLSGASSRPPMGLGPAPYPWLADSWPHPSRSPSAPEPRGSCCPSNPDPDDRYYNEAGISLYLAQTARGTAAPTEGPVYSTIDPAGEELQTFHGGFPPNPSGDPGAWSQYAPPEWSQGDSGARGGKVKLLGKAVQMPSLNWPEALPPPPPSCELSCLEGPEEDLEGSSEPEEWCPPMSERSHLTEPSSTGGCLVPPSQGEAPSPTSSYGQQSTATLTPSPPDPPQPPTDIPHLHQMPRRVPLGPSSPLSVSQPTLSSHEGRPAGLGAGPTASHHLNPSPVPSTASSAPGRTRQVPGEMTPPLQGPRARIRKPKAVPYRREHSPGGLSPDLPPPPLPPPEEETSWALGLRAAGSMSSLERERERSGERMVQAGLLGAQRGPHLDEEAWLPYSRPSFLSRGQGPNTCSTAGSNSSRGSGSSRGSRGPGRSRSRSRSQSQRPGQKCGEVRAREPR

Target ORF information:

RefSeq Version	XM_014113325.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113325.1

ORF Insert Sequence:

ATGCTGCGCT ACCTGCTGAA GACACTGCTG CAGATGAACT TGTTCGCGGA CTCCCTGGCG 
GGGGACATCT CCAACTCCAG CGACCTGCTC TTCGGCTTCA ACTCCTCGGT GGCGGCGCTC 
AACCACAGCC TGCTGCCCCC CGGCGACCCC TCTTTCAACG CGTCAAGGGT CGAGCCGGAG 
GACGCGATGC CGCGCATCGT GGAGCAGCCG CCAGATCTGC TGGTGTCCCG GGGCGAGCCG 
GCCACGCTGC CCTGCCGCGC CGAGGGCCGG CCCCGGCCCA ACATCGAGTG GTACAAGAAC 
GGGGCGCGGG TGGCCACTGC GCGCGAGGAC CCGCGCGCGC ACCGCCTGCT GCTGCCCAGC 
GGCGCCCTCT TCTTCCCCCG CATCGTGCAC GGGCGCCGCG CGCGCCCCGA CGAGGGTGTC 
TACACTTGCG TGGCGCGCAA CTACCTGGGG GCAGCGGCCA GCAGAAATGC CTCGCTGGAA 
GTGGCGGTCC TCCGTGATGA TTTCCGGCAG TCTCCTGGAA ACGTGGTGGT GGCAGTGGGC 
GAGCCAGCGG TGATGGAATG CGTTCCCCCC CGTGGCCACC CGGAGCCTTC CGTGTCCTGG 
AAGAAGGACA GTGCAAGACT CAGAGAGGAG GAGGGAAGGA TCACGATTCG TGGAGGGAAG 
CTGATGATGT CACATACACT CAAGAGTGAT GCGGGGATGT ATGTGTGCGT GGCCTCCAAC 
ATGGCAGGAG AACGGGAGAG TGGGGCAGCT GAACTTGTGG TACTGGAGCG ACCCTCATTC 
CTGCGTAGAC CAGTCAACCA GGTGGTCCTG GCAGATGCCC CCGTGGATTT CCCATGTGAG 
GTGCAGGGAG ATCCCCCACC CCGTCTACGC TGGCGCAAGG AGGATGGGGA ACTGCCCACC 
GGCAGGTATG AGATCCGGAG TGACCACAGC CTTTGGATCG GCCGCGTGAC TGCCGCCGAC 
GAGGGGACCT ACACCTGTGT GGCTGAGAAC AGCGTGGGCC GCGCCGAAGC ATCTGGCTCC 
CTCAGTGTTC ACGTCCCACC CCAGCTGGTG ACCCAGCCGC AGGACCAGAT GGCAGCTCCT 
GGAGAGAGTG TGGCTTTCCA ATGCGAGACC AAAGGAAACC CCCCACCTGC CATCTTCTGG 
CAGAAGGAGG GAAGTCAGGT TCTACTTTTC CCCAGCCAGC CGCTTCAGCC CAAGGGGCGC 
TTCTCTGTGT CTCCCAGAGG CCAGCTCAAC ATTACGGATG TGCAGAGCGG GGATGCTGGC 
TACTACGTGT GTCAGGCCGT TAGTGTGGCC GGGAGCGTCC TGGCTAAGGC CCTGCTGGAG 
GTAAAAAGAG CCTCCTCCTT GGATGGGCTG CCTCCTATCA TCCTCCAGGG ACCAGCCAAT 
CAGACGCTGG CACTTGGCTC CCCTGTGTGG CTGCCATGCA GAGTGACCGG GAACCCTCAA 
CCGAGTGTCC GATGGAAGAA GGATGGGCAG TGGCTGCAGG GGGACGACCT CCAGCTCAAC 
CTAATGGCCA ATGGTACTCT GTACATCGCC AGCGTGCAGG AGATGGACCT GGGTTTCTAC 
AGCTGTGTGG CCAAGAGTTC CATGGGGGAG GCCACATGGA GCAGCTGGCT TAGGAGGCGG 
GATTATCTCC TCGCTGCAGA AGATTGGGGA GTATCACCAG AGCCCCCTAC AGAACCCAGT 
ACCCCTCCAG GGCCTCCCTC TCAGCCAGTG GTCACTGAGA TCACCAAGAA CAGCATTACC 
CTGACCTGGA AGCCCAACCC GCAGGCGGGG GCCACAGTCA CCTCTTATGT GATAGAGGCC 
TTCAGCCAAG CAGCTGGCAA CACGTGGCGG ACAGTGGCAG ATGGTGTGCA GCTGGAGACA 
CACACGGTCA GTGGTCTGCA GCCCAACACC ATCTATCTGT TTCTGGTGCG AGCTGTGGGA 
GCCTGGGGCC TCAGTGAGCC CAGCCCCGTC TCTGAGCCCA TCCGCACCCA GGACAGCAAC 
CCATCCAGGC CAGTGGAGGA CCCATGGAGA GGCCAGCGGG GACTGGCTGA AGTGGCTGTG 
CATGTGCAGG AGCCCGTAGT CCTTGGGCTC CGGACCTTGC AGGTGTCCTG GACTGTAGAT 
GGCCCAGTCC AGCTGGTGCA AGGTTTCCGG GTGTCTTGGA GGGTAGCAGG TCCTGATGGG 
GGAAGCTGGA CAGTACTGGA CCTACAGTCC CCAAGCCAGC AAAGTACTGT GCTAAGAGGA 
CTCCCCCCAG GGACCCAAAT TCAGATCAAA GTGCAAGCCC AAGGCCAGGA GGGGCTGGGG 
CCTGAAAGTC CCTTTGTGAC CAGGAGCATT CCGGAGGAAG CCCCCAGTGG CCCCCCCCAG 
GGAGTGACAG TGGCCTTGGG GGGTGAAGGC AACAGCAGTG TCACGGTGTC CTGGGAACCT 
CCCCTCCCCT CCCAGCAAAA TGGGGTGATC AAGGAATACC AGATCTGGTG CCTGGGCAAT 
GAGAGCCGCT TCCACCTCAA CCGGTCTGCG GCAGGCTGGG CACGCTCCGC CGTGCTCCGG 
GGACTGCTGC CCGGCCTTCT CTACCGGACC CGGGTCGCGG CGGCCACCAG CGCCGGCGTG 
GGCGTGGCCA GCGTCCCGGT GTCGGTGCAG CTGCCCTCCC CGCCGGAGTC GGAGCCCGGG 
CTCGAGGTGC GCCCGGGGCT GGCGGAGCGC CTGGCCAGGG TGCTGCGGGC GCCTGCCTTC 
CTGGCGGGCA GCGGGGCCGC CTGCGGGGCG CTGCTGCTCG CGCTCTGCGC CGTCCTCTAC 
CGGCGCCGGA GGCAGCGCAA GGAGCTCAGT CACTACACGG CCTCCTTTGC CTACACACCG 
GCAGTGTCCT TCCCACACTC AGAGGGCCTC TCTGGAGCCA GTTCCAGGCC ACCCATGGGC 
CTCGGCCCCG CCCCCTACCC TTGGCTGGCC GACTCGTGGC CCCACCCATC TCGAAGCCCC 
TCAGCCCCGG AGCCCAGGGG GAGCTGCTGC CCCAGCAATC CTGACCCAGA CGACAGATAT 
TACAATGAAG CAGGGATCTC CCTGTACCTG GCTCAGACGG CCCGAGGCAC TGCCGCCCCT 
ACAGAGGGTC CTGTCTACAG CACCATTGAC CCAGCTGGGG AGGAGCTGCA GACCTTCCAC 
GGGGGGTTCC CCCCAAATCC CTCGGGGGAC CCAGGCGCCT GGAGCCAATA TGCTCCTCCA 
GAATGGAGCC AGGGGGACAG TGGAGCCAGG GGAGGCAAAG TAAAGCTTCT GGGAAAAGCT 
GTGCAGATGC CCTCTCTCAA CTGGCCGGAA GCCCTGCCAC CACCTCCCCC TTCCTGTGAA 
CTGAGCTGCC TAGAGGGGCC TGAGGAAGAT CTGGAAGGCA GCTCAGAGCC AGAAGAGTGG 
TGCCCACCAA TGTCTGAGAG GAGCCATCTG ACAGAGCCCA GCTCCACTGG AGGGTGCTTG 
GTCCCTCCAT CCCAAGGGGA AGCCCCCTCT CCCACATCTT CCTATGGACA GCAGTCCACA 
GCTACTCTTA CCCCCTCACC TCCTGACCCT CCCCAGCCCC CCACTGACAT CCCCCATCTC 
CACCAGATGC CCAGGAGGGT GCCCCTGGGG CCAAGTTCCC CTCTCAGTGT ATCCCAGCCC 
ACTCTGAGTA GCCATGAAGG GAGGCCTGCT GGCCTGGGTG CTGGCCCCAC AGCCTCCCAT 
CACCTCAACC CCAGTCCTGT CCCTAGTACA GCCAGCAGTG CCCCAGGGAG AACCCGGCAG 
GTGCCTGGGG AGATGACCCC TCCGCTTCAA GGGCCCCGGG CCCGAATCCG GAAGCCCAAG 
GCTGTTCCCT ACCGGCGGGA GCACAGTCCT GGCGGTCTCT CCCCAGATTT GCCCCCACCA 
CCCTTGCCGC CACCAGAGGA AGAGACGAGC TGGGCCTTAG GGCTGAGAGC AGCAGGCAGC 
ATGTCCTCCC TGGAACGAGA GCGGGAGCGC AGTGGGGAGA GAATGGTGCA GGCTGGGCTC 
CTGGGGGCCC AGCGGGGTCC CCACCTGGAT GAAGAGGCCT GGCTCCCTTA CAGCCGACCG 
AGCTTCCTGT CCCGGGGCCA GGGGCCCAAC ACCTGTTCCA CAGCCGGCAG CAACTCTTCG 
AGAGGCTCTG GCAGCTCTCG AGGCTCCCGG GGACCTGGAC GGAGCCGGAG CCGGAGCCGG 
AGCCAGAGCC AGAGGCCAGG ACAGAAATGT GGAGAGGTGC GGGCTAGGGA ACCAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO3 ( XM_014113326.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968801.1 Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe36280
Clone ID Related Accession (Same CDS sequence)	XM_014113326.1
Accession Version	XM_014113326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	roundabout homolog 3 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGCT ACCTGCTGAA GACACTGCTG CAGATGAACT TGTTCGCGGA CTCCCTGGCG 
GGGGACATCT CCAACTCCAG CGACCTGCTC TTCGGCTTCA ACTCCTCGGT GGCGGCGCTC 
AACCACAGCC TGCTGCCCCC CGGCGACCCC TCTTTCAACG CGTCAAGGGT CGAGCCGGAG 
GACGCGATGC CGCGCATCGT GGAGCAGCCG CCAGATCTGC TGGTGTCCCG GGGCGAGCCG 
GCCACGCTGC CCTGCCGCGC CGAGGGCCGG CCCCGGCCCA ACATCGAGTG GTACAAGAAC 
GGGGCGCGGG TGGCCACTGC GCGCGAGGAC CCGCGCGCGC ACCGCCTGCT GCTGCCCAGC 
GGCGCCCTCT TCTTCCCCCG CATCGTGCAC GGGCGCCGCG CGCGCCCCGA CGAGGGTGTC 
TACACTTGCG TGGCGCGCAA CTACCTGGGG GCAGCGGCCA GCAGAAATGC CTCGCTGGAA 
GTGGCGGTCC TCCGTGATGA TTTCCGGCAG TCTCCTGGAA ACGTGGTGGT GGCAGTGGGC 
GAGCCAGCGG TGATGGAATG CGTTCCCCCC CGTGGCCACC CGGAGCCTTC CGTGTCCTGG 
AAGAAGGACA GTGCAAGACT CAGAGAGGAG GAGGGAAGGA TCACGATTCG TGGAGGGAAG 
CTGATGATGT CACATACACT CAAGAGTGAT GCGGGGATGT ATGTGTGCGT GGCCTCCAAC 
ATGGCAGGAG AACGGGAGAG TGGGGCAGCT GAACTTGTGG TACTGGAGCG ACCCTCATTC 
CTGCGTAGAC CAGTCAACCA GGTGGTCCTG GCAGATGCCC CCGTGGATTT CCCATGTGAG 
GTGCAGGGAG ATCCCCCACC CCGTCTACGC TGGCGCAAGG AGGATGGGGA ACTGCCCACC 
GGCAGGTATG AGATCCGGAG TGACCACAGC CTTTGGATCG GCCGCGTGAC TGCCGCCGAC 
GAGGGGACCT ACACCTGTGT GGCTGAGAAC AGCGTGGGCC GCGCCGAAGC ATCTGGCTCC 
CTCAGTGTTC ACGTCCCACC CCAGCTGGTG ACCCAGCCGC AGGACCAGAT GGCAGCTCCT 
GGAGAGAGTG TGGCTTTCCA ATGCGAGACC AAAGGAAACC CCCCACCTGC CATCTTCTGG 
CAGAAGGAGG GAAGTCAGGT TCTACTTTTC CCCAGCCAGC CGCTTCAGCC CAAGGGGCGC 
TTCTCTGTGT CTCCCAGAGG CCAGCTCAAC ATTACGGATG TGCAGAGCGG GGATGCTGGC 
TACTACGTGT GTCAGGCCGT TAGTGTGGCC GGGAGCGTCC TGGCTAAGGC CCTGCTGGAG 
GTAAAAAGAG CCTCCTCCTT GGATGGGCTG CCTCCTATCA TCCTCCAGGG ACCAGCCAAT 
CAGACGCTGG CACTTGGCTC CCCTGTGTGG CTGCCATGCA GAGTGACCGG GAACCCTCAA 
CCGAGTGTCC GATGGAAGAA GGATGGGCAG TGGCTGCAGG GGGACGACCT CCAGCTCAAC 
CTAATGGCCA ATGGTACTCT GTACATCGCC AGCGTGCAGG AGATGGACCT GGGTTTCTAC 
AGCTGTGTGG CCAAGAGTTC CATGGGGGAG GCCACATGGA GCAGCTGGCT TAGGAGGCGG 
GATTATCTCC TCGCTGCAGA AGATTGGGGA GTATCACCAG AGCCCCCTAC AGAACCCAGT 
ACCCCTCCAG GGCCTCCCTC TCAGCCAGTG GTCACTGAGA TCACCAAGAA CAGCATTACC 
CTGACCTGGA AGCCCAACCC GCAGGCGGGG GCCACAGTCA CCTCTTATGT GATAGAGGCC 
TTCAGCCAAG CAGCTGGCAA CACGTGGCGG ACAGTGGCAG ATGGTGTGCA GCTGGAGACA 
CACACGGTCA GTGGTCTGCA GCCCAACACC ATCTATCTGT TTCTGGTGCG AGCTGTGGGA 
GCCTGGGGCC TCAGTGAGCC CAGCCCCGTC TCTGAGCCCA TCCGCACCCA GGACAGCAAC 
CCATCCAGGC CAGTGGAGGA CCCATGGAGA GGCCAGCGGG GACTGGCTGA AGTGGCTGTG 
CATGTGCAGG AGCCCGTAGT CCTTGGGCTC CGGACCTTGC AGGTGTCCTG GACTGTAGAT 
GGCCCAGTCC AGCTGGTGCA AGGTTTCCGG GTGTCTTGGA GGGTAGCAGG TCCTGATGGG 
GGAAGCTGGA CAGTACTGGA CCTACAGTCC CCAAGCCAGC AAAGTACTGT GCTAAGAGGA 
CTCCCCCCAG GGACCCAAAT TCAGATCAAA GTGCAAGCCC AAGGCCAGGA GGGGCTGGGG 
CCTGAAAGTC CCTTTGTGAC CAGGAGCATT CCGGAGGAAG CCCCCAGTGG CCCCCCCCAG 
GGAGTGACAG TGGCCTTGGG GGGTGAAGGC AACAGCAGTG TCACGGTGTC CTGGGAACCT 
CCCCTCCCCT CCCAGCAAAA TGGGGTGATC AAGGAATACC AGATCTGGTG CCTGGGCAAT 
GAGAGCCGCT TCCACCTCAA CCGGTCTGCG GCAGGCTGGG CACGCTCCGC CGTGCTCCGG 
GGACTGCTGC CCGGCCTTCT CTACCGGACC CGGGTCGCGG CGGCCACCAG CGCCGGCGTG 
GGCGTGGCCA GCGTCCCGGT GTCGGTGCAG CTGCCCTCCC CGCCGGAGTC GGAGCCCGGG 
CTCGAGGTGC GCCCGGGGCT GGCGGAGCGC CTGGCCAGGG TGCTGCGGGC GCCTGCCTTC 
CTGGCGGGCA GCGGGGCCGC CTGCGGGGCG CTGCTGCTCG CGCTCTGCGC CGTCCTCTAC 
CGGCGCCGGA GGCAGCGCAA GGAGCTCAGT CACTACACGG CCTCCTTTGC CTACACACCG 
GCAGTGTCCT TCCCACACTC AGAGGGCCTC TCTGGAGCCA GTTCCAGGCC ACCCATGGGC 
CTCGGCCCCG CCCCCTACCC TTGGCTGGCC GACTCGTGGC CCCACCCATC TCGAAGCCCC 
TCAGCCCCGG AGCCCAGGGG GAGCTGCTGC CCCAGCAATC CTGACCCAGA CGACAGATAT 
TACAATGAAG CAGGGATCTC CCTGTACCTG GCTCAGACGG CCCGAGGCAC TGCCGCCCCT 
ACAGAGGGTC CTGTCTACAG CACCATTGAC CCAGCTGGGG AGGAGCTGCA GACCTTCCAC 
GGGGGGTTCC CCCCAAATCC CTCGGGGGAC CCAGGCGCCT GGAGCCAATA TGCTCCTCCA 
GAATGGAGCC AGGGGGACAG TGGAGCCAGG GGAGGCAAAG TAAAGCTTCT GGGAAAAGCT 
GTGCAGATGC CCTCTCTCAA CTGGCCGGAA GCCCTGCCAC CACCTCCCCC TTCCTGTGAA 
CTGAGCTGCC TAGAGGGGCC TGAGGAAGAT CTGGAAGGCA GCTCAGAGCC AGAAGAGTGG 
TGCCCACCAA TGTCTGAGAG GAGCCATCTG ACAGAGCCCA GCTCCACTGG AGGGTGCTTG 
GTCCCTCCAT CCCAAGGGGA AGCCCCCTCT CCCACATCTT CCTATGGACA GCAGTCCACA 
GCTACTCTTA CCCCCTCACC TCCTGACCCT CCCCAGCCCC CCACTGACAT CCCCCATCTC 
CACCAGATGC CCAGGAGGGT GCCCCTGGGG CCAAGTTCCC CTCTCAGTGT ATCCCAGCCC 
ACTCTGAGTA GCCATGAAGG GAGGCCTGCT GGCCTGGGTG CTGGCCCCAC AGCCTCCCAT 
CACCTCAACC CCAGTCCTGT CCCTAGTACA GCCAGCAGTG CCCCAGGGAG AACCCGGCAG 
GTGCCTGGGG AGATGACCCC TCCGCTTCAA GGGCCCCGGG CCCGAATCCG GAAGCCCAAG 
GCTGTTCCCT ACCGGCGGGA GCACAGTCCT GGCGGTCTCT CCCCAGATTT GCCCCCACCA 
CCCTTGCCGC CACCAGAGGA AGAGACGAGC TGGGCCTTAG GGCTGAGAGC AGCAGGCAGC 
ATGTCCTCCC TGGAACGAGA GCGGGAGCGC AGTGGGGAGA GAATGGTGCA GGCTGGGCTC 
CTGGGGGCCC AGCGGGGTCC CCACCTGGAT GAAGAGGCCT GGCTCCCTTA CAGCCGACCG 
AGCTTCCTGT CCCGGGGCCA GGGGCCCAAC ACCTGTTCCA CAGCCGGCAG CAACTCTTCG 
AGAGGCTCTG GCAGCTCTCG AGGCTCCCGG GGACCTGGAC GGAGCCGGAG CCGGAGCCGG 
AGCCAGAGCC AGAGGCCAGG ACAGAAATGT GGAGAGGAAC CAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968801.1
CDS	194..4381
Translation	MLRYLLKTLLQMNLFADSLAGDISNSSDLLFGFNSSVAALNHSLLPPGDPSFNASRVEPEDAMPRIVEQPPDLLVSRGEPATLPCRAEGRPRPNIEWYKNGARVATAREDPRAHRLLLPSGALFFPRIVHGRRARPDEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVMECVPPRGHPEPSVSWKKDSARLREEEGRITIRGGKLMMSHTLKSDAGMYVCVASNMAGERESGAAELVVLERPSFLRRPVNQVVLADAPVDFPCEVQGDPPPRLRWRKEDGELPTGRYEIRSDHSLWIGRVTAADEGTYTCVAENSVGRAEASGSLSVHVPPQLVTQPQDQMAAPGESVAFQCETKGNPPPAIFWQKEGSQVLLFPSQPLQPKGRFSVSPRGQLNITDVQSGDAGYYVCQAVSVAGSVLAKALLEVKRASSLDGLPPIILQGPANQTLALGSPVWLPCRVTGNPQPSVRWKKDGQWLQGDDLQLNLMANGTLYIASVQEMDLGFYSCVAKSSMGEATWSSWLRRRDYLLAAEDWGVSPEPPTEPSTPPGPPSQPVVTEITKNSITLTWKPNPQAGATVTSYVIEAFSQAAGNTWRTVADGVQLETHTVSGLQPNTIYLFLVRAVGAWGLSEPSPVSEPIRTQDSNPSRPVEDPWRGQRGLAEVAVHVQEPVVLGLRTLQVSWTVDGPVQLVQGFRVSWRVAGPDGGSWTVLDLQSPSQQSTVLRGLPPGTQIQIKVQAQGQEGLGPESPFVTRSIPEEAPSGPPQGVTVALGGEGNSSVTVSWEPPLPSQQNGVIKEYQIWCLGNESRFHLNRSAAGWARSAVLRGLLPGLLYRTRVAAATSAGVGVASVPVSVQLPSPPESEPGLEVRPGLAERLARVLRAPAFLAGSGAACGALLLALCAVLYRRRRQRKELSHYTASFAYTPAVSFPHSEGLSGASSRPPMGLGPAPYPWLADSWPHPSRSPSAPEPRGSCCPSNPDPDDRYYNEAGISLYLAQTARGTAAPTEGPVYSTIDPAGEELQTFHGGFPPNPSGDPGAWSQYAPPEWSQGDSGARGGKVKLLGKAVQMPSLNWPEALPPPPPSCELSCLEGPEEDLEGSSEPEEWCPPMSERSHLTEPSSTGGCLVPPSQGEAPSPTSSYGQQSTATLTPSPPDPPQPPTDIPHLHQMPRRVPLGPSSPLSVSQPTLSSHEGRPAGLGAGPTASHHLNPSPVPSTASSAPGRTRQVPGEMTPPLQGPRARIRKPKAVPYRREHSPGGLSPDLPPPPLPPPEEETSWALGLRAAGSMSSLERERERSGERMVQAGLLGAQRGPHLDEEAWLPYSRPSFLSRGQGPNTCSTAGSNSSRGSGSSRGSRGPGRSRSRSRSQSQRPGQKCGEEPR

Target ORF information:

RefSeq Version	XM_014113326.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113326.1

ORF Insert Sequence:

ATGCTGCGCT ACCTGCTGAA GACACTGCTG CAGATGAACT TGTTCGCGGA CTCCCTGGCG 
GGGGACATCT CCAACTCCAG CGACCTGCTC TTCGGCTTCA ACTCCTCGGT GGCGGCGCTC 
AACCACAGCC TGCTGCCCCC CGGCGACCCC TCTTTCAACG CGTCAAGGGT CGAGCCGGAG 
GACGCGATGC CGCGCATCGT GGAGCAGCCG CCAGATCTGC TGGTGTCCCG GGGCGAGCCG 
GCCACGCTGC CCTGCCGCGC CGAGGGCCGG CCCCGGCCCA ACATCGAGTG GTACAAGAAC 
GGGGCGCGGG TGGCCACTGC GCGCGAGGAC CCGCGCGCGC ACCGCCTGCT GCTGCCCAGC 
GGCGCCCTCT TCTTCCCCCG CATCGTGCAC GGGCGCCGCG CGCGCCCCGA CGAGGGTGTC 
TACACTTGCG TGGCGCGCAA CTACCTGGGG GCAGCGGCCA GCAGAAATGC CTCGCTGGAA 
GTGGCGGTCC TCCGTGATGA TTTCCGGCAG TCTCCTGGAA ACGTGGTGGT GGCAGTGGGC 
GAGCCAGCGG TGATGGAATG CGTTCCCCCC CGTGGCCACC CGGAGCCTTC CGTGTCCTGG 
AAGAAGGACA GTGCAAGACT CAGAGAGGAG GAGGGAAGGA TCACGATTCG TGGAGGGAAG 
CTGATGATGT CACATACACT CAAGAGTGAT GCGGGGATGT ATGTGTGCGT GGCCTCCAAC 
ATGGCAGGAG AACGGGAGAG TGGGGCAGCT GAACTTGTGG TACTGGAGCG ACCCTCATTC 
CTGCGTAGAC CAGTCAACCA GGTGGTCCTG GCAGATGCCC CCGTGGATTT CCCATGTGAG 
GTGCAGGGAG ATCCCCCACC CCGTCTACGC TGGCGCAAGG AGGATGGGGA ACTGCCCACC 
GGCAGGTATG AGATCCGGAG TGACCACAGC CTTTGGATCG GCCGCGTGAC TGCCGCCGAC 
GAGGGGACCT ACACCTGTGT GGCTGAGAAC AGCGTGGGCC GCGCCGAAGC ATCTGGCTCC 
CTCAGTGTTC ACGTCCCACC CCAGCTGGTG ACCCAGCCGC AGGACCAGAT GGCAGCTCCT 
GGAGAGAGTG TGGCTTTCCA ATGCGAGACC AAAGGAAACC CCCCACCTGC CATCTTCTGG 
CAGAAGGAGG GAAGTCAGGT TCTACTTTTC CCCAGCCAGC CGCTTCAGCC CAAGGGGCGC 
TTCTCTGTGT CTCCCAGAGG CCAGCTCAAC ATTACGGATG TGCAGAGCGG GGATGCTGGC 
TACTACGTGT GTCAGGCCGT TAGTGTGGCC GGGAGCGTCC TGGCTAAGGC CCTGCTGGAG 
GTAAAAAGAG CCTCCTCCTT GGATGGGCTG CCTCCTATCA TCCTCCAGGG ACCAGCCAAT 
CAGACGCTGG CACTTGGCTC CCCTGTGTGG CTGCCATGCA GAGTGACCGG GAACCCTCAA 
CCGAGTGTCC GATGGAAGAA GGATGGGCAG TGGCTGCAGG GGGACGACCT CCAGCTCAAC 
CTAATGGCCA ATGGTACTCT GTACATCGCC AGCGTGCAGG AGATGGACCT GGGTTTCTAC 
AGCTGTGTGG CCAAGAGTTC CATGGGGGAG GCCACATGGA GCAGCTGGCT TAGGAGGCGG 
GATTATCTCC TCGCTGCAGA AGATTGGGGA GTATCACCAG AGCCCCCTAC AGAACCCAGT 
ACCCCTCCAG GGCCTCCCTC TCAGCCAGTG GTCACTGAGA TCACCAAGAA CAGCATTACC 
CTGACCTGGA AGCCCAACCC GCAGGCGGGG GCCACAGTCA CCTCTTATGT GATAGAGGCC 
TTCAGCCAAG CAGCTGGCAA CACGTGGCGG ACAGTGGCAG ATGGTGTGCA GCTGGAGACA 
CACACGGTCA GTGGTCTGCA GCCCAACACC ATCTATCTGT TTCTGGTGCG AGCTGTGGGA 
GCCTGGGGCC TCAGTGAGCC CAGCCCCGTC TCTGAGCCCA TCCGCACCCA GGACAGCAAC 
CCATCCAGGC CAGTGGAGGA CCCATGGAGA GGCCAGCGGG GACTGGCTGA AGTGGCTGTG 
CATGTGCAGG AGCCCGTAGT CCTTGGGCTC CGGACCTTGC AGGTGTCCTG GACTGTAGAT 
GGCCCAGTCC AGCTGGTGCA AGGTTTCCGG GTGTCTTGGA GGGTAGCAGG TCCTGATGGG 
GGAAGCTGGA CAGTACTGGA CCTACAGTCC CCAAGCCAGC AAAGTACTGT GCTAAGAGGA 
CTCCCCCCAG GGACCCAAAT TCAGATCAAA GTGCAAGCCC AAGGCCAGGA GGGGCTGGGG 
CCTGAAAGTC CCTTTGTGAC CAGGAGCATT CCGGAGGAAG CCCCCAGTGG CCCCCCCCAG 
GGAGTGACAG TGGCCTTGGG GGGTGAAGGC AACAGCAGTG TCACGGTGTC CTGGGAACCT 
CCCCTCCCCT CCCAGCAAAA TGGGGTGATC AAGGAATACC AGATCTGGTG CCTGGGCAAT 
GAGAGCCGCT TCCACCTCAA CCGGTCTGCG GCAGGCTGGG CACGCTCCGC CGTGCTCCGG 
GGACTGCTGC CCGGCCTTCT CTACCGGACC CGGGTCGCGG CGGCCACCAG CGCCGGCGTG 
GGCGTGGCCA GCGTCCCGGT GTCGGTGCAG CTGCCCTCCC CGCCGGAGTC GGAGCCCGGG 
CTCGAGGTGC GCCCGGGGCT GGCGGAGCGC CTGGCCAGGG TGCTGCGGGC GCCTGCCTTC 
CTGGCGGGCA GCGGGGCCGC CTGCGGGGCG CTGCTGCTCG CGCTCTGCGC CGTCCTCTAC 
CGGCGCCGGA GGCAGCGCAA GGAGCTCAGT CACTACACGG CCTCCTTTGC CTACACACCG 
GCAGTGTCCT TCCCACACTC AGAGGGCCTC TCTGGAGCCA GTTCCAGGCC ACCCATGGGC 
CTCGGCCCCG CCCCCTACCC TTGGCTGGCC GACTCGTGGC CCCACCCATC TCGAAGCCCC 
TCAGCCCCGG AGCCCAGGGG GAGCTGCTGC CCCAGCAATC CTGACCCAGA CGACAGATAT 
TACAATGAAG CAGGGATCTC CCTGTACCTG GCTCAGACGG CCCGAGGCAC TGCCGCCCCT 
ACAGAGGGTC CTGTCTACAG CACCATTGAC CCAGCTGGGG AGGAGCTGCA GACCTTCCAC 
GGGGGGTTCC CCCCAAATCC CTCGGGGGAC CCAGGCGCCT GGAGCCAATA TGCTCCTCCA 
GAATGGAGCC AGGGGGACAG TGGAGCCAGG GGAGGCAAAG TAAAGCTTCT GGGAAAAGCT 
GTGCAGATGC CCTCTCTCAA CTGGCCGGAA GCCCTGCCAC CACCTCCCCC TTCCTGTGAA 
CTGAGCTGCC TAGAGGGGCC TGAGGAAGAT CTGGAAGGCA GCTCAGAGCC AGAAGAGTGG 
TGCCCACCAA TGTCTGAGAG GAGCCATCTG ACAGAGCCCA GCTCCACTGG AGGGTGCTTG 
GTCCCTCCAT CCCAAGGGGA AGCCCCCTCT CCCACATCTT CCTATGGACA GCAGTCCACA 
GCTACTCTTA CCCCCTCACC TCCTGACCCT CCCCAGCCCC CCACTGACAT CCCCCATCTC 
CACCAGATGC CCAGGAGGGT GCCCCTGGGG CCAAGTTCCC CTCTCAGTGT ATCCCAGCCC 
ACTCTGAGTA GCCATGAAGG GAGGCCTGCT GGCCTGGGTG CTGGCCCCAC AGCCTCCCAT 
CACCTCAACC CCAGTCCTGT CCCTAGTACA GCCAGCAGTG CCCCAGGGAG AACCCGGCAG 
GTGCCTGGGG AGATGACCCC TCCGCTTCAA GGGCCCCGGG CCCGAATCCG GAAGCCCAAG 
GCTGTTCCCT ACCGGCGGGA GCACAGTCCT GGCGGTCTCT CCCCAGATTT GCCCCCACCA 
CCCTTGCCGC CACCAGAGGA AGAGACGAGC TGGGCCTTAG GGCTGAGAGC AGCAGGCAGC 
ATGTCCTCCC TGGAACGAGA GCGGGAGCGC AGTGGGGAGA GAATGGTGCA GGCTGGGCTC 
CTGGGGGCCC AGCGGGGTCC CCACCTGGAT GAAGAGGCCT GGCTCCCTTA CAGCCGACCG 
AGCTTCCTGT CCCGGGGCCA GGGGCCCAAC ACCTGTTCCA CAGCCGGCAG CAACTCTTCG 
AGAGGCTCTG GCAGCTCTCG AGGCTCCCGG GGACCTGGAC GGAGCCGGAG CCGGAGCCGG 
AGCCAGAGCC AGAGGCCAGG ACAGAAATGT GGAGAGGAAC CAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO3 ( XM_014113327.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968802.1 Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe36281
Clone ID Related Accession (Same CDS sequence)	XM_014113327.1
Accession Version	XM_014113327.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	roundabout homolog 3 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGCT ACCTGCTGAA GACACTGCTG CAGATGAACT TGTTCGCGGA CTCCCTGGCG 
GGGGACATCT CCAACTCCAG CGACCTGCTC TTCGGCTTCA ACTCCTCGGT GGCGGCGCTC 
AACCACAGCC TGCTGCCCCC CGGCGACCCC TCTTTCAACG CGTCAAGGGT CGAGCCGGAG 
GACGCGATGC CGCGCATCGT GGAGCAGCCG CCAGATCTGC TGGTGTCCCG GGGCGAGCCG 
GCCACGCTGC CCTGCCGCGC CGAGGGCCGG CCCCGGCCCA ACATCGAGTG GTACAAGAAC 
GGGGCGCGGG TGGCCACTGC GCGCGAGGAC CCGCGCGCGC ACCGCCTGCT GCTGCCCAGC 
GGCGCCCTCT TCTTCCCCCG CATCGTGCAC GGGCGCCGCG CGCGCCCCGA CGAGGGTGTC 
TACACTTGCG TGGCGCGCAA CTACCTGGGG GCAGCGGCCA GCAGAAATGC CTCGCTGGAA 
GTGGCGGTCC TCCGTGATGA TTTCCGGCAG TCTCCTGGAA ACGTGGTGGT GGCAGTGGGC 
GAGCCAGCGG TGATGGAATG CGTTCCCCCC CGTGGCCACC CGGAGCCTTC CGTGTCCTGG 
AAGAAGGACA GTGCAAGACT CAGAGAGGAG GAGGGAAGGA TCACGATTCG TGGAGGGAAG 
CTGATGATGT CACATACACT CAAGAGTGAT GCGGGGATGT ATGTGTGCGT GGCCTCCAAC 
ATGGCAGGAG AACGGGAGAG TGGGGCAGCT GAACTTGTGG TACTGGAGCG ACCCTCATTC 
CTGCGTAGAC CAGTCAACCA GGTGGTCCTG GCAGATGCCC CCGTGGATTT CCCATGTGAG 
GTGCAGGGAG ATCCCCCACC CCGTCTACGC TGGCGCAAGG AGGATGGGGA ACTGCCCACC 
GGCAGGTATG AGATCCGGAG TGACCACAGC CTTTGGATCG GCCGCGTGAC TGCCGCCGAC 
GAGGGGACCT ACACCTGTGT GGCTGAGAAC AGCGTGGGCC GCGCCGAAGC ATCTGGCTCC 
CTCAGTGTTC ACGTCCCACC CCAGCTGGTG ACCCAGCCGC AGGACCAGAT GGCAGCTCCT 
GGAGAGAGTG TGGCTTTCCA ATGCGAGACC AAAGGAAACC CCCCACCTGC CATCTTCTGG 
CAGAAGGAGG GAAGTCAGGT TCTACTTTTC CCCAGCCAGC CGCTTCAGCC CAAGGGGCGC 
TTCTCTGTGT CTCCCAGAGG CCAGCTCAAC ATTACGGATG TGCAGAGCGG GGATGCTGGC 
TACTACGTGT GTCAGGCCGT TAGTGTGGCC GGGAGCGTCC TGGCTAAGGC CCTGCTGGAG 
GTAAAAAGAG CCTCCTCCTT GGATGGGCTG CCTCCTATCA TCCTCCAGGG ACCAGCCAAT 
CAGACGCTGG CACTTGGCTC CCCTGTGTGG CTGCCATGCA GAGTGACCGG GAACCCTCAA 
CCGAGTGTCC GATGGAAGAA GGATGGGCAG TGGCTGCAGG GGGACGACCT CCAGCTCAAC 
CTAATGGCCA ATGGTACTCT GTACATCGCC AGCGTGCAGG AGATGGACCT GGGTTTCTAC 
AGCTGTGTGG CCAAGAGTTC CATGGGGGAG GCCACATGGA GCAGCTGGCT TAGGAGGCGG 
GATTATCTCC TCGCTGCAGA AGATTGGGGA GTATCACCAG AGCCCCCTAC AGAACCCAGT 
ACCCCTCCAG GGCCTCCCTC TCAGCCAGTG GTCACTGAGA TCACCAAGAA CAGCATTACC 
CTGACCTGGA AGCCCAACCC GCAGGCGGGG GCCACAGTCA CCTCTTATGT GATAGAGGCC 
TTCAGCCAAG CAGCTGGCAA CACGTGGCGG ACAGTGGCAG ATGGTGTGCA GCTGGAGACA 
CACACGGTCA GTGGTCTGCA GCCCAACACC ATCTATCTGT TTCTGGTGCG AGCTGTGGGA 
GCCTGGGGCC TCAGTGAGCC CAGCCCCGTC TCTGAGCCCA TCCGCACCCA GGACAGCAAC 
CCATCCAGGC CAGTGGAGGA CCCATGGAGA GGCCAGCGGG GACTGGCTGA AGTGGCTGTG 
CATGTGCAGG AGCCCGTAGT CCTTGGGCTC CGGACCTTGC AGGTGTCCTG GACTGTAGAT 
GGCCCAGTCC AGCTGGTGCA AGGTTTCCGG GTGTCTTGGA GGGTAGCAGG TCCTGATGGG 
GGAAGCTGGA CAGTACTGGA CCTACAGTCC CCAAGCCAGC AAAGTACTGT GCTAAGAGGA 
CTCCCCCCAG GGACCCAAAT TCAGATCAAA GTGCAAGCCC AAGGCCAGGA GGGGCTGGGG 
CCTGAAAGTC CCTTTGTGAC CAGGAGCATT CCGGAGGAAG CCCCCAGTGG CCCCCCCCAG 
GGAGTGACAG TGGCCTTGGG GGGTGAAGGC AACAGCAGTG TCACGGTGTC CTGGGAACCT 
CCCCTCCCCT CCCAGCAAAA TGGGGTGATC AAGGAATACC AGATCTGGTG CCTGGGCAAT 
GAGAGCCGCT TCCACCTCAA CCGGTCTGCG GCAGGCTGGG CACGCTCCGC CGTGCTCCGG 
GGACTGCTGC CCGGCCTTCT CTACCGGACC CGGGTCGCGG CGGCCACCAG CGCCGGCGTG 
GGCGTGGCCA GCGTCCCGGT GTCGGTGCAG CTGCCCTCCC CGCCGGAGTC GGAGCCCGGG 
CTCGAGGTGC GCCCGGGGCT GGCGGAGCGC CTGGCCAGGG TGCTGCGGGC GCCTGCCTTC 
CTGGCGGGCA GCGGGGCCGC CTGCGGGGCG CTGCTGCTCG CGCTCTGCGC CGTCCTCTAC 
CGGCGCCGGA GGCAGCGCAA GGAGCTCAGT CACTACACGG CCTCCTTTGC CTACACACCG 
GCAGTGTCCT TCCCACACTC AGAGGGCCTC TCTGGAGCCA GTTCCAGGCC ACCCATGGGC 
CTCGGCCCCG CCCCCTACCC TTGGCTGGCC GACTCGTGGC CCCACCCATC TCGAAGCCCC 
TCAGCCCCGG AGCCCAGGGG GAGCTGCTGC CCCAGCAATC CTGACCCAGA CGACAGATAT 
TACAATGAAG CAGGGATCTC CCTGTACCTG GCTCAGACGG CCCGAGGCAC TGCCGCCCCT 
ACAGAGGGTC CTGTCTACAG CACCATTGAC CCAGCTGGGG AGGAGCTGCA GACCTTCCAC 
GGGGGGTTCC CCCCAAATCC CTCGGGGGAC CCAGGCGCCT GGAGCCAATA TGCTCCTCCA 
GAATGGAGCC AGGGGGACAG TGGAGCCAGG GGAGGCAAAG TAAAGCTTCT GGGAAAAGCT 
GTGCAGATGC CCTCTCTCAA CTGGCCGGAA GCCCTGCCAC CACCTCCCCC TTCCTGTGAA 
CTGAGCTGCC TAGAGGGGCC TGAGGAAGAT CTGGAAGGCA GCTCAGAGCC AGAAGAGTGG 
TGCCCACCAA TGTCTGAGAG GAGCCATCTG ACAGAGCCCA GCTCCACTGG AGGGTGCTTG 
GTCCCTCCAT CCCAAGGGGA AGCCCCCTCT CCCACATCTT CCTATGGACA GCAGTCCACA 
GCTACTCTTA CCCCCTCACC TCCTGACCCT CCCCAGCCCC CCACTGACAT CCCCCATCTC 
CACCAGATGC CCAGGAGGGT GCCCCTGGGG CCAAGTTCCC CTCTCAGTGT ATCCCAGCCC 
ACTCTGAGTA GCCATGAAGG GAGGCCTGCT GGCCTGGGTG CTGGCCCCAC AGCCTCCCAT 
CACCTCAACC CCAGTCCTGT CCCTAGTACA GCCAGCAGTG CCCCAGGGAG AACCCGGCAG 
GTGCCTGGGG AGATGACCCC TCCGCTTCAA GGGCCCCGGG CCCGAATCCG GAAGCCCAAG 
GCTGTTCCCT ACCGGCGGGA GCACAGTCCT GGCGATTTGC CCCCACCACC CTTGCCGCCA 
CCAGAGGAAG AGACGAGCTG GGCCTTAGGG CTGAGAGCAG CAGGCAGCAT GTCCTCCCTG 
GAACGAGAGC GGGAGCGCAG TGGGGAGAGA ATGGTGCAGG CTGGGCTCCT GGGGGCCCAG 
CGGGGTCCCC ACCTGGATGA AGAGGCCTGG CTCCCTTACA GCCGACCGAG CTTCCTGTCC 
CGGGGCCAGG GGCCCAACAC CTGTTCCACA GCCGGCAGCA ACTCTTCGAG AGGCTCTGGC 
AGCTCTCGAG GCTCCCGGGG ACCTGGACGG AGCCGGAGCC GGAGCCGGAG CCAGAGCCAG 
AGGCCAGGAC AGAAATGTGG AGAGGTGCGG GCTAGGGAAC CAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968802.1
CDS	193..4380
Translation	MLRYLLKTLLQMNLFADSLAGDISNSSDLLFGFNSSVAALNHSLLPPGDPSFNASRVEPEDAMPRIVEQPPDLLVSRGEPATLPCRAEGRPRPNIEWYKNGARVATAREDPRAHRLLLPSGALFFPRIVHGRRARPDEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVMECVPPRGHPEPSVSWKKDSARLREEEGRITIRGGKLMMSHTLKSDAGMYVCVASNMAGERESGAAELVVLERPSFLRRPVNQVVLADAPVDFPCEVQGDPPPRLRWRKEDGELPTGRYEIRSDHSLWIGRVTAADEGTYTCVAENSVGRAEASGSLSVHVPPQLVTQPQDQMAAPGESVAFQCETKGNPPPAIFWQKEGSQVLLFPSQPLQPKGRFSVSPRGQLNITDVQSGDAGYYVCQAVSVAGSVLAKALLEVKRASSLDGLPPIILQGPANQTLALGSPVWLPCRVTGNPQPSVRWKKDGQWLQGDDLQLNLMANGTLYIASVQEMDLGFYSCVAKSSMGEATWSSWLRRRDYLLAAEDWGVSPEPPTEPSTPPGPPSQPVVTEITKNSITLTWKPNPQAGATVTSYVIEAFSQAAGNTWRTVADGVQLETHTVSGLQPNTIYLFLVRAVGAWGLSEPSPVSEPIRTQDSNPSRPVEDPWRGQRGLAEVAVHVQEPVVLGLRTLQVSWTVDGPVQLVQGFRVSWRVAGPDGGSWTVLDLQSPSQQSTVLRGLPPGTQIQIKVQAQGQEGLGPESPFVTRSIPEEAPSGPPQGVTVALGGEGNSSVTVSWEPPLPSQQNGVIKEYQIWCLGNESRFHLNRSAAGWARSAVLRGLLPGLLYRTRVAAATSAGVGVASVPVSVQLPSPPESEPGLEVRPGLAERLARVLRAPAFLAGSGAACGALLLALCAVLYRRRRQRKELSHYTASFAYTPAVSFPHSEGLSGASSRPPMGLGPAPYPWLADSWPHPSRSPSAPEPRGSCCPSNPDPDDRYYNEAGISLYLAQTARGTAAPTEGPVYSTIDPAGEELQTFHGGFPPNPSGDPGAWSQYAPPEWSQGDSGARGGKVKLLGKAVQMPSLNWPEALPPPPPSCELSCLEGPEEDLEGSSEPEEWCPPMSERSHLTEPSSTGGCLVPPSQGEAPSPTSSYGQQSTATLTPSPPDPPQPPTDIPHLHQMPRRVPLGPSSPLSVSQPTLSSHEGRPAGLGAGPTASHHLNPSPVPSTASSAPGRTRQVPGEMTPPLQGPRARIRKPKAVPYRREHSPGDLPPPPLPPPEEETSWALGLRAAGSMSSLERERERSGERMVQAGLLGAQRGPHLDEEAWLPYSRPSFLSRGQGPNTCSTAGSNSSRGSGSSRGSRGPGRSRSRSRSQSQRPGQKCGEVRAREPR

Target ORF information:

RefSeq Version	XM_014113327.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113327.1

ORF Insert Sequence:

ATGCTGCGCT ACCTGCTGAA GACACTGCTG CAGATGAACT TGTTCGCGGA CTCCCTGGCG 
GGGGACATCT CCAACTCCAG CGACCTGCTC TTCGGCTTCA ACTCCTCGGT GGCGGCGCTC 
AACCACAGCC TGCTGCCCCC CGGCGACCCC TCTTTCAACG CGTCAAGGGT CGAGCCGGAG 
GACGCGATGC CGCGCATCGT GGAGCAGCCG CCAGATCTGC TGGTGTCCCG GGGCGAGCCG 
GCCACGCTGC CCTGCCGCGC CGAGGGCCGG CCCCGGCCCA ACATCGAGTG GTACAAGAAC 
GGGGCGCGGG TGGCCACTGC GCGCGAGGAC CCGCGCGCGC ACCGCCTGCT GCTGCCCAGC 
GGCGCCCTCT TCTTCCCCCG CATCGTGCAC GGGCGCCGCG CGCGCCCCGA CGAGGGTGTC 
TACACTTGCG TGGCGCGCAA CTACCTGGGG GCAGCGGCCA GCAGAAATGC CTCGCTGGAA 
GTGGCGGTCC TCCGTGATGA TTTCCGGCAG TCTCCTGGAA ACGTGGTGGT GGCAGTGGGC 
GAGCCAGCGG TGATGGAATG CGTTCCCCCC CGTGGCCACC CGGAGCCTTC CGTGTCCTGG 
AAGAAGGACA GTGCAAGACT CAGAGAGGAG GAGGGAAGGA TCACGATTCG TGGAGGGAAG 
CTGATGATGT CACATACACT CAAGAGTGAT GCGGGGATGT ATGTGTGCGT GGCCTCCAAC 
ATGGCAGGAG AACGGGAGAG TGGGGCAGCT GAACTTGTGG TACTGGAGCG ACCCTCATTC 
CTGCGTAGAC CAGTCAACCA GGTGGTCCTG GCAGATGCCC CCGTGGATTT CCCATGTGAG 
GTGCAGGGAG ATCCCCCACC CCGTCTACGC TGGCGCAAGG AGGATGGGGA ACTGCCCACC 
GGCAGGTATG AGATCCGGAG TGACCACAGC CTTTGGATCG GCCGCGTGAC TGCCGCCGAC 
GAGGGGACCT ACACCTGTGT GGCTGAGAAC AGCGTGGGCC GCGCCGAAGC ATCTGGCTCC 
CTCAGTGTTC ACGTCCCACC CCAGCTGGTG ACCCAGCCGC AGGACCAGAT GGCAGCTCCT 
GGAGAGAGTG TGGCTTTCCA ATGCGAGACC AAAGGAAACC CCCCACCTGC CATCTTCTGG 
CAGAAGGAGG GAAGTCAGGT TCTACTTTTC CCCAGCCAGC CGCTTCAGCC CAAGGGGCGC 
TTCTCTGTGT CTCCCAGAGG CCAGCTCAAC ATTACGGATG TGCAGAGCGG GGATGCTGGC 
TACTACGTGT GTCAGGCCGT TAGTGTGGCC GGGAGCGTCC TGGCTAAGGC CCTGCTGGAG 
GTAAAAAGAG CCTCCTCCTT GGATGGGCTG CCTCCTATCA TCCTCCAGGG ACCAGCCAAT 
CAGACGCTGG CACTTGGCTC CCCTGTGTGG CTGCCATGCA GAGTGACCGG GAACCCTCAA 
CCGAGTGTCC GATGGAAGAA GGATGGGCAG TGGCTGCAGG GGGACGACCT CCAGCTCAAC 
CTAATGGCCA ATGGTACTCT GTACATCGCC AGCGTGCAGG AGATGGACCT GGGTTTCTAC 
AGCTGTGTGG CCAAGAGTTC CATGGGGGAG GCCACATGGA GCAGCTGGCT TAGGAGGCGG 
GATTATCTCC TCGCTGCAGA AGATTGGGGA GTATCACCAG AGCCCCCTAC AGAACCCAGT 
ACCCCTCCAG GGCCTCCCTC TCAGCCAGTG GTCACTGAGA TCACCAAGAA CAGCATTACC 
CTGACCTGGA AGCCCAACCC GCAGGCGGGG GCCACAGTCA CCTCTTATGT GATAGAGGCC 
TTCAGCCAAG CAGCTGGCAA CACGTGGCGG ACAGTGGCAG ATGGTGTGCA GCTGGAGACA 
CACACGGTCA GTGGTCTGCA GCCCAACACC ATCTATCTGT TTCTGGTGCG AGCTGTGGGA 
GCCTGGGGCC TCAGTGAGCC CAGCCCCGTC TCTGAGCCCA TCCGCACCCA GGACAGCAAC 
CCATCCAGGC CAGTGGAGGA CCCATGGAGA GGCCAGCGGG GACTGGCTGA AGTGGCTGTG 
CATGTGCAGG AGCCCGTAGT CCTTGGGCTC CGGACCTTGC AGGTGTCCTG GACTGTAGAT 
GGCCCAGTCC AGCTGGTGCA AGGTTTCCGG GTGTCTTGGA GGGTAGCAGG TCCTGATGGG 
GGAAGCTGGA CAGTACTGGA CCTACAGTCC CCAAGCCAGC AAAGTACTGT GCTAAGAGGA 
CTCCCCCCAG GGACCCAAAT TCAGATCAAA GTGCAAGCCC AAGGCCAGGA GGGGCTGGGG 
CCTGAAAGTC CCTTTGTGAC CAGGAGCATT CCGGAGGAAG CCCCCAGTGG CCCCCCCCAG 
GGAGTGACAG TGGCCTTGGG GGGTGAAGGC AACAGCAGTG TCACGGTGTC CTGGGAACCT 
CCCCTCCCCT CCCAGCAAAA TGGGGTGATC AAGGAATACC AGATCTGGTG CCTGGGCAAT 
GAGAGCCGCT TCCACCTCAA CCGGTCTGCG GCAGGCTGGG CACGCTCCGC CGTGCTCCGG 
GGACTGCTGC CCGGCCTTCT CTACCGGACC CGGGTCGCGG CGGCCACCAG CGCCGGCGTG 
GGCGTGGCCA GCGTCCCGGT GTCGGTGCAG CTGCCCTCCC CGCCGGAGTC GGAGCCCGGG 
CTCGAGGTGC GCCCGGGGCT GGCGGAGCGC CTGGCCAGGG TGCTGCGGGC GCCTGCCTTC 
CTGGCGGGCA GCGGGGCCGC CTGCGGGGCG CTGCTGCTCG CGCTCTGCGC CGTCCTCTAC 
CGGCGCCGGA GGCAGCGCAA GGAGCTCAGT CACTACACGG CCTCCTTTGC CTACACACCG 
GCAGTGTCCT TCCCACACTC AGAGGGCCTC TCTGGAGCCA GTTCCAGGCC ACCCATGGGC 
CTCGGCCCCG CCCCCTACCC TTGGCTGGCC GACTCGTGGC CCCACCCATC TCGAAGCCCC 
TCAGCCCCGG AGCCCAGGGG GAGCTGCTGC CCCAGCAATC CTGACCCAGA CGACAGATAT 
TACAATGAAG CAGGGATCTC CCTGTACCTG GCTCAGACGG CCCGAGGCAC TGCCGCCCCT 
ACAGAGGGTC CTGTCTACAG CACCATTGAC CCAGCTGGGG AGGAGCTGCA GACCTTCCAC 
GGGGGGTTCC CCCCAAATCC CTCGGGGGAC CCAGGCGCCT GGAGCCAATA TGCTCCTCCA 
GAATGGAGCC AGGGGGACAG TGGAGCCAGG GGAGGCAAAG TAAAGCTTCT GGGAAAAGCT 
GTGCAGATGC CCTCTCTCAA CTGGCCGGAA GCCCTGCCAC CACCTCCCCC TTCCTGTGAA 
CTGAGCTGCC TAGAGGGGCC TGAGGAAGAT CTGGAAGGCA GCTCAGAGCC AGAAGAGTGG 
TGCCCACCAA TGTCTGAGAG GAGCCATCTG ACAGAGCCCA GCTCCACTGG AGGGTGCTTG 
GTCCCTCCAT CCCAAGGGGA AGCCCCCTCT CCCACATCTT CCTATGGACA GCAGTCCACA 
GCTACTCTTA CCCCCTCACC TCCTGACCCT CCCCAGCCCC CCACTGACAT CCCCCATCTC 
CACCAGATGC CCAGGAGGGT GCCCCTGGGG CCAAGTTCCC CTCTCAGTGT ATCCCAGCCC 
ACTCTGAGTA GCCATGAAGG GAGGCCTGCT GGCCTGGGTG CTGGCCCCAC AGCCTCCCAT 
CACCTCAACC CCAGTCCTGT CCCTAGTACA GCCAGCAGTG CCCCAGGGAG AACCCGGCAG 
GTGCCTGGGG AGATGACCCC TCCGCTTCAA GGGCCCCGGG CCCGAATCCG GAAGCCCAAG 
GCTGTTCCCT ACCGGCGGGA GCACAGTCCT GGCGATTTGC CCCCACCACC CTTGCCGCCA 
CCAGAGGAAG AGACGAGCTG GGCCTTAGGG CTGAGAGCAG CAGGCAGCAT GTCCTCCCTG 
GAACGAGAGC GGGAGCGCAG TGGGGAGAGA ATGGTGCAGG CTGGGCTCCT GGGGGCCCAG 
CGGGGTCCCC ACCTGGATGA AGAGGCCTGG CTCCCTTACA GCCGACCGAG CTTCCTGTCC 
CGGGGCCAGG GGCCCAACAC CTGTTCCACA GCCGGCAGCA ACTCTTCGAG AGGCTCTGGC 
AGCTCTCGAG GCTCCCGGGG ACCTGGACGG AGCCGGAGCC GGAGCCGGAG CCAGAGCCAG 
AGGCCAGGAC AGAAATGTGG AGAGGTGCGG GCTAGGGAAC CAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO3 ( XM_014113328.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968803.1 Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X4, mRNA.

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CloneID	OCe36282
Clone ID Related Accession (Same CDS sequence)	XM_014113328.1 , XM_014113328.2
Accession Version	XM_014113328.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	roundabout homolog 3 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGCT ACCTGCTGAA GACACTGCTG CAGATGAACT TGTTCGCGGA CTCCCTGGCG 
GGGGACATCT CCAACTCCAG CGACCTGCTC TTCGGCTTCA ACTCCTCGGT GGCGGCGCTC 
AACCACAGCC TGCTGCCCCC CGGCGACCCC TCTTTCAACG CGTCAAGGGT CGAGCCGGAG 
GACGCGATGC CGCGCATCGT GGAGCAGCCG CCAGATCTGC TGGTGTCCCG GGGCGAGCCG 
GCCACGCTGC CCTGCCGCGC CGAGGGCCGG CCCCGGCCCA ACATCGAGTG GTACAAGAAC 
GGGGCGCGGG TGGCCACTGC GCGCGAGGAC CCGCGCGCGC ACCGCCTGCT GCTGCCCAGC 
GGCGCCCTCT TCTTCCCCCG CATCGTGCAC GGGCGCCGCG CGCGCCCCGA CGAGGGTGTC 
TACACTTGCG TGGCGCGCAA CTACCTGGGG GCAGCGGCCA GCAGAAATGC CTCGCTGGAA 
GTGGCGGTCC TCCGTGATGA TTTCCGGCAG TCTCCTGGAA ACGTGGTGGT GGCAGTGGGC 
GAGCCAGCGG TGATGGAATG CGTTCCCCCC CGTGGCCACC CGGAGCCTTC CGTGTCCTGG 
AAGAAGGACA GTGCAAGACT CAGAGAGGAG GAGGGAAGGA TCACGATTCG TGGAGGGAAG 
CTGATGATGT CACATACACT CAAGAGTGAT GCGGGGATGT ATGTGTGCGT GGCCTCCAAC 
ATGGCAGGAG AACGGGAGAG TGGGGCAGCT GAACTTGTGG TACTGGAGCG ACCCTCATTC 
CTGCGTAGAC CAGTCAACCA GGTGGTCCTG GCAGATGCCC CCGTGGATTT CCCATGTGAG 
GTGCAGGGAG ATCCCCCACC CCGTCTACGC TGGCGCAAGG AGGATGGGGA ACTGCCCACC 
GGCAGGTATG AGATCCGGAG TGACCACAGC CTTTGGATCG GCCGCGTGAC TGCCGCCGAC 
GAGGGGACCT ACACCTGTGT GGCTGAGAAC AGCGTGGGCC GCGCCGAAGC ATCTGGCTCC 
CTCAGTGTTC ACGTCCCACC CCAGCTGGTG ACCCAGCCGC AGGACCAGAT GGCAGCTCCT 
GGAGAGAGTG TGGCTTTCCA ATGCGAGACC AAAGGAAACC CCCCACCTGC CATCTTCTGG 
CAGAAGGAGG GAAGTCAGGT TCTACTTTTC CCCAGCCAGC CGCTTCAGCC CAAGGGGCGC 
TTCTCTGTGT CTCCCAGAGG CCAGCTCAAC ATTACGGATG TGCAGAGCGG GGATGCTGGC 
TACTACGTGT GTCAGGCCGT TAGTGTGGCC GGGAGCGTCC TGGCTAAGGC CCTGCTGGAG 
GTAAAAAGAG CCTCCTCCTT GGATGGGCTG CCTCCTATCA TCCTCCAGGG ACCAGCCAAT 
CAGACGCTGG CACTTGGCTC CCCTGTGTGG CTGCCATGCA GAGTGACCGG GAACCCTCAA 
CCGAGTGTCC GATGGAAGAA GGATGGGCAG TGGCTGCAGG GGGACGACCT CCAGCTCAAC 
CTAATGGCCA ATGGTACTCT GTACATCGCC AGCGTGCAGG AGATGGACCT GGGTTTCTAC 
AGCTGTGTGG CCAAGAGTTC CATGGGGGAG GCCACATGGA GCAGCTGGCT TAGGAGGCGG 
GATTGGGGAG TATCACCAGA GCCCCCTACA GAACCCAGTA CCCCTCCAGG GCCTCCCTCT 
CAGCCAGTGG TCACTGAGAT CACCAAGAAC AGCATTACCC TGACCTGGAA GCCCAACCCG 
CAGGCGGGGG CCACAGTCAC CTCTTATGTG ATAGAGGCCT TCAGCCAAGC AGCTGGCAAC 
ACGTGGCGGA CAGTGGCAGA TGGTGTGCAG CTGGAGACAC ACACGGTCAG TGGTCTGCAG 
CCCAACACCA TCTATCTGTT TCTGGTGCGA GCTGTGGGAG CCTGGGGCCT CAGTGAGCCC 
AGCCCCGTCT CTGAGCCCAT CCGCACCCAG GACAGCAACC CATCCAGGCC AGTGGAGGAC 
CCATGGAGAG GCCAGCGGGG ACTGGCTGAA GTGGCTGTGC ATGTGCAGGA GCCCGTAGTC 
CTTGGGCTCC GGACCTTGCA GGTGTCCTGG ACTGTAGATG GCCCAGTCCA GCTGGTGCAA 
GGTTTCCGGG TGTCTTGGAG GGTAGCAGGT CCTGATGGGG GAAGCTGGAC AGTACTGGAC 
CTACAGTCCC CAAGCCAGCA AAGTACTGTG CTAAGAGGAC TCCCCCCAGG GACCCAAATT 
CAGATCAAAG TGCAAGCCCA AGGCCAGGAG GGGCTGGGGC CTGAAAGTCC CTTTGTGACC 
AGGAGCATTC CGGAGGAAGC CCCCAGTGGC CCCCCCCAGG GAGTGACAGT GGCCTTGGGG 
GGTGAAGGCA ACAGCAGTGT CACGGTGTCC TGGGAACCTC CCCTCCCCTC CCAGCAAAAT 
GGGGTGATCA AGGAATACCA GATCTGGTGC CTGGGCAATG AGAGCCGCTT CCACCTCAAC 
CGGTCTGCGG CAGGCTGGGC ACGCTCCGCC GTGCTCCGGG GACTGCTGCC CGGCCTTCTC 
TACCGGACCC GGGTCGCGGC GGCCACCAGC GCCGGCGTGG GCGTGGCCAG CGTCCCGGTG 
TCGGTGCAGC TGCCCTCCCC GCCGGAGTCG GAGCCCGGGC TCGAGGTGCG CCCGGGGCTG 
GCGGAGCGCC TGGCCAGGGT GCTGCGGGCG CCTGCCTTCC TGGCGGGCAG CGGGGCCGCC 
TGCGGGGCGC TGCTGCTCGC GCTCTGCGCC GTCCTCTACC GGCGCCGGAG GCAGCGCAAG 
GAGCTCAGTC ACTACACGGC CTCCTTTGCC TACACACCGG CAGTGTCCTT CCCACACTCA 
GAGGGCCTCT CTGGAGCCAG TTCCAGGCCA CCCATGGGCC TCGGCCCCGC CCCCTACCCT 
TGGCTGGCCG ACTCGTGGCC CCACCCATCT CGAAGCCCCT CAGCCCCGGA GCCCAGGGGG 
AGCTGCTGCC CCAGCAATCC TGACCCAGAC GACAGATATT ACAATGAAGC AGGGATCTCC 
CTGTACCTGG CTCAGACGGC CCGAGGCACT GCCGCCCCTA CAGAGGGTCC TGTCTACAGC 
ACCATTGACC CAGCTGGGGA GGAGCTGCAG ACCTTCCACG GGGGGTTCCC CCCAAATCCC 
TCGGGGGACC CAGGCGCCTG GAGCCAATAT GCTCCTCCAG AATGGAGCCA GGGGGACAGT 
GGAGCCAGGG GAGGCAAAGT AAAGCTTCTG GGAAAAGCTG TGCAGATGCC CTCTCTCAAC 
TGGCCGGAAG CCCTGCCACC ACCTCCCCCT TCCTGTGAAC TGAGCTGCCT AGAGGGGCCT 
GAGGAAGATC TGGAAGGCAG CTCAGAGCCA GAAGAGTGGT GCCCACCAAT GTCTGAGAGG 
AGCCATCTGA CAGAGCCCAG CTCCACTGGA GGGTGCTTGG TCCCTCCATC CCAAGGGGAA 
GCCCCCTCTC CCACATCTTC CTATGGACAG CAGTCCACAG CTACTCTTAC CCCCTCACCT 
CCTGACCCTC CCCAGCCCCC CACTGACATC CCCCATCTCC ACCAGATGCC CAGGAGGGTG 
CCCCTGGGGC CAAGTTCCCC TCTCAGTGTA TCCCAGCCCA CTCTGAGTAG CCATGAAGGG 
AGGCCTGCTG GCCTGGGTGC TGGCCCCACA GCCTCCCATC ACCTCAACCC CAGTCCTGTC 
CCTAGTACAG CCAGCAGTGC CCCAGGGAGA ACCCGGCAGG TGCCTGGGGA GATGACCCCT 
CCGCTTCAAG GGCCCCGGGC CCGAATCCGG AAGCCCAAGG CTGTTCCCTA CCGGCGGGAG 
CACAGTCCTG GCGGTCTCTC CCCAGATTTG CCCCCACCAC CCTTGCCGCC ACCAGAGGAA 
GAGACGAGCT GGGCCTTAGG GCTGAGAGCA GCAGGCAGCA TGTCCTCCCT GGAACGAGAG 
CGGGAGCGCA GTGGGGAGAG AATGGTGCAG GCTGGGCTCC TGGGGGCCCA GCGGGGTCCC 
CACCTGGATG AAGAGGCCTG GCTCCCTTAC AGCCGACCGA GCTTCCTGTC CCGGGGCCAG 
GGGCCCAACA CCTGTTCCAC AGCCGGCAGC AACTCTTCGA GAGGCTCTGG CAGCTCTCGA 
GGCTCCCGGG GACCTGGACG GAGCCGGAGC CGGAGCCGGA GCCAGAGCCA GAGGCCAGGA 
CAGAAATGTG GAGAGGTGCG GGCTAGGGAA CCAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968803.1
CDS	195..4373
Translation	MLRYLLKTLLQMNLFADSLAGDISNSSDLLFGFNSSVAALNHSLLPPGDPSFNASRVEPEDAMPRIVEQPPDLLVSRGEPATLPCRAEGRPRPNIEWYKNGARVATAREDPRAHRLLLPSGALFFPRIVHGRRARPDEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVMECVPPRGHPEPSVSWKKDSARLREEEGRITIRGGKLMMSHTLKSDAGMYVCVASNMAGERESGAAELVVLERPSFLRRPVNQVVLADAPVDFPCEVQGDPPPRLRWRKEDGELPTGRYEIRSDHSLWIGRVTAADEGTYTCVAENSVGRAEASGSLSVHVPPQLVTQPQDQMAAPGESVAFQCETKGNPPPAIFWQKEGSQVLLFPSQPLQPKGRFSVSPRGQLNITDVQSGDAGYYVCQAVSVAGSVLAKALLEVKRASSLDGLPPIILQGPANQTLALGSPVWLPCRVTGNPQPSVRWKKDGQWLQGDDLQLNLMANGTLYIASVQEMDLGFYSCVAKSSMGEATWSSWLRRRDWGVSPEPPTEPSTPPGPPSQPVVTEITKNSITLTWKPNPQAGATVTSYVIEAFSQAAGNTWRTVADGVQLETHTVSGLQPNTIYLFLVRAVGAWGLSEPSPVSEPIRTQDSNPSRPVEDPWRGQRGLAEVAVHVQEPVVLGLRTLQVSWTVDGPVQLVQGFRVSWRVAGPDGGSWTVLDLQSPSQQSTVLRGLPPGTQIQIKVQAQGQEGLGPESPFVTRSIPEEAPSGPPQGVTVALGGEGNSSVTVSWEPPLPSQQNGVIKEYQIWCLGNESRFHLNRSAAGWARSAVLRGLLPGLLYRTRVAAATSAGVGVASVPVSVQLPSPPESEPGLEVRPGLAERLARVLRAPAFLAGSGAACGALLLALCAVLYRRRRQRKELSHYTASFAYTPAVSFPHSEGLSGASSRPPMGLGPAPYPWLADSWPHPSRSPSAPEPRGSCCPSNPDPDDRYYNEAGISLYLAQTARGTAAPTEGPVYSTIDPAGEELQTFHGGFPPNPSGDPGAWSQYAPPEWSQGDSGARGGKVKLLGKAVQMPSLNWPEALPPPPPSCELSCLEGPEEDLEGSSEPEEWCPPMSERSHLTEPSSTGGCLVPPSQGEAPSPTSSYGQQSTATLTPSPPDPPQPPTDIPHLHQMPRRVPLGPSSPLSVSQPTLSSHEGRPAGLGAGPTASHHLNPSPVPSTASSAPGRTRQVPGEMTPPLQGPRARIRKPKAVPYRREHSPGGLSPDLPPPPLPPPEEETSWALGLRAAGSMSSLERERERSGERMVQAGLLGAQRGPHLDEEAWLPYSRPSFLSRGQGPNTCSTAGSNSSRGSGSSRGSRGPGRSRSRSRSQSQRPGQKCGEVRAREPR

Target ORF information:

RefSeq Version	XM_014113328.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113328.1

ORF Insert Sequence:

ATGCTGCGCT ACCTGCTGAA GACACTGCTG CAGATGAACT TGTTCGCGGA CTCCCTGGCG 
GGGGACATCT CCAACTCCAG CGACCTGCTC TTCGGCTTCA ACTCCTCGGT GGCGGCGCTC 
AACCACAGCC TGCTGCCCCC CGGCGACCCC TCTTTCAACG CGTCAAGGGT CGAGCCGGAG 
GACGCGATGC CGCGCATCGT GGAGCAGCCG CCAGATCTGC TGGTGTCCCG GGGCGAGCCG 
GCCACGCTGC CCTGCCGCGC CGAGGGCCGG CCCCGGCCCA ACATCGAGTG GTACAAGAAC 
GGGGCGCGGG TGGCCACTGC GCGCGAGGAC CCGCGCGCGC ACCGCCTGCT GCTGCCCAGC 
GGCGCCCTCT TCTTCCCCCG CATCGTGCAC GGGCGCCGCG CGCGCCCCGA CGAGGGTGTC 
TACACTTGCG TGGCGCGCAA CTACCTGGGG GCAGCGGCCA GCAGAAATGC CTCGCTGGAA 
GTGGCGGTCC TCCGTGATGA TTTCCGGCAG TCTCCTGGAA ACGTGGTGGT GGCAGTGGGC 
GAGCCAGCGG TGATGGAATG CGTTCCCCCC CGTGGCCACC CGGAGCCTTC CGTGTCCTGG 
AAGAAGGACA GTGCAAGACT CAGAGAGGAG GAGGGAAGGA TCACGATTCG TGGAGGGAAG 
CTGATGATGT CACATACACT CAAGAGTGAT GCGGGGATGT ATGTGTGCGT GGCCTCCAAC 
ATGGCAGGAG AACGGGAGAG TGGGGCAGCT GAACTTGTGG TACTGGAGCG ACCCTCATTC 
CTGCGTAGAC CAGTCAACCA GGTGGTCCTG GCAGATGCCC CCGTGGATTT CCCATGTGAG 
GTGCAGGGAG ATCCCCCACC CCGTCTACGC TGGCGCAAGG AGGATGGGGA ACTGCCCACC 
GGCAGGTATG AGATCCGGAG TGACCACAGC CTTTGGATCG GCCGCGTGAC TGCCGCCGAC 
GAGGGGACCT ACACCTGTGT GGCTGAGAAC AGCGTGGGCC GCGCCGAAGC ATCTGGCTCC 
CTCAGTGTTC ACGTCCCACC CCAGCTGGTG ACCCAGCCGC AGGACCAGAT GGCAGCTCCT 
GGAGAGAGTG TGGCTTTCCA ATGCGAGACC AAAGGAAACC CCCCACCTGC CATCTTCTGG 
CAGAAGGAGG GAAGTCAGGT TCTACTTTTC CCCAGCCAGC CGCTTCAGCC CAAGGGGCGC 
TTCTCTGTGT CTCCCAGAGG CCAGCTCAAC ATTACGGATG TGCAGAGCGG GGATGCTGGC 
TACTACGTGT GTCAGGCCGT TAGTGTGGCC GGGAGCGTCC TGGCTAAGGC CCTGCTGGAG 
GTAAAAAGAG CCTCCTCCTT GGATGGGCTG CCTCCTATCA TCCTCCAGGG ACCAGCCAAT 
CAGACGCTGG CACTTGGCTC CCCTGTGTGG CTGCCATGCA GAGTGACCGG GAACCCTCAA 
CCGAGTGTCC GATGGAAGAA GGATGGGCAG TGGCTGCAGG GGGACGACCT CCAGCTCAAC 
CTAATGGCCA ATGGTACTCT GTACATCGCC AGCGTGCAGG AGATGGACCT GGGTTTCTAC 
AGCTGTGTGG CCAAGAGTTC CATGGGGGAG GCCACATGGA GCAGCTGGCT TAGGAGGCGG 
GATTGGGGAG TATCACCAGA GCCCCCTACA GAACCCAGTA CCCCTCCAGG GCCTCCCTCT 
CAGCCAGTGG TCACTGAGAT CACCAAGAAC AGCATTACCC TGACCTGGAA GCCCAACCCG 
CAGGCGGGGG CCACAGTCAC CTCTTATGTG ATAGAGGCCT TCAGCCAAGC AGCTGGCAAC 
ACGTGGCGGA CAGTGGCAGA TGGTGTGCAG CTGGAGACAC ACACGGTCAG TGGTCTGCAG 
CCCAACACCA TCTATCTGTT TCTGGTGCGA GCTGTGGGAG CCTGGGGCCT CAGTGAGCCC 
AGCCCCGTCT CTGAGCCCAT CCGCACCCAG GACAGCAACC CATCCAGGCC AGTGGAGGAC 
CCATGGAGAG GCCAGCGGGG ACTGGCTGAA GTGGCTGTGC ATGTGCAGGA GCCCGTAGTC 
CTTGGGCTCC GGACCTTGCA GGTGTCCTGG ACTGTAGATG GCCCAGTCCA GCTGGTGCAA 
GGTTTCCGGG TGTCTTGGAG GGTAGCAGGT CCTGATGGGG GAAGCTGGAC AGTACTGGAC 
CTACAGTCCC CAAGCCAGCA AAGTACTGTG CTAAGAGGAC TCCCCCCAGG GACCCAAATT 
CAGATCAAAG TGCAAGCCCA AGGCCAGGAG GGGCTGGGGC CTGAAAGTCC CTTTGTGACC 
AGGAGCATTC CGGAGGAAGC CCCCAGTGGC CCCCCCCAGG GAGTGACAGT GGCCTTGGGG 
GGTGAAGGCA ACAGCAGTGT CACGGTGTCC TGGGAACCTC CCCTCCCCTC CCAGCAAAAT 
GGGGTGATCA AGGAATACCA GATCTGGTGC CTGGGCAATG AGAGCCGCTT CCACCTCAAC 
CGGTCTGCGG CAGGCTGGGC ACGCTCCGCC GTGCTCCGGG GACTGCTGCC CGGCCTTCTC 
TACCGGACCC GGGTCGCGGC GGCCACCAGC GCCGGCGTGG GCGTGGCCAG CGTCCCGGTG 
TCGGTGCAGC TGCCCTCCCC GCCGGAGTCG GAGCCCGGGC TCGAGGTGCG CCCGGGGCTG 
GCGGAGCGCC TGGCCAGGGT GCTGCGGGCG CCTGCCTTCC TGGCGGGCAG CGGGGCCGCC 
TGCGGGGCGC TGCTGCTCGC GCTCTGCGCC GTCCTCTACC GGCGCCGGAG GCAGCGCAAG 
GAGCTCAGTC ACTACACGGC CTCCTTTGCC TACACACCGG CAGTGTCCTT CCCACACTCA 
GAGGGCCTCT CTGGAGCCAG TTCCAGGCCA CCCATGGGCC TCGGCCCCGC CCCCTACCCT 
TGGCTGGCCG ACTCGTGGCC CCACCCATCT CGAAGCCCCT CAGCCCCGGA GCCCAGGGGG 
AGCTGCTGCC CCAGCAATCC TGACCCAGAC GACAGATATT ACAATGAAGC AGGGATCTCC 
CTGTACCTGG CTCAGACGGC CCGAGGCACT GCCGCCCCTA CAGAGGGTCC TGTCTACAGC 
ACCATTGACC CAGCTGGGGA GGAGCTGCAG ACCTTCCACG GGGGGTTCCC CCCAAATCCC 
TCGGGGGACC CAGGCGCCTG GAGCCAATAT GCTCCTCCAG AATGGAGCCA GGGGGACAGT 
GGAGCCAGGG GAGGCAAAGT AAAGCTTCTG GGAAAAGCTG TGCAGATGCC CTCTCTCAAC 
TGGCCGGAAG CCCTGCCACC ACCTCCCCCT TCCTGTGAAC TGAGCTGCCT AGAGGGGCCT 
GAGGAAGATC TGGAAGGCAG CTCAGAGCCA GAAGAGTGGT GCCCACCAAT GTCTGAGAGG 
AGCCATCTGA CAGAGCCCAG CTCCACTGGA GGGTGCTTGG TCCCTCCATC CCAAGGGGAA 
GCCCCCTCTC CCACATCTTC CTATGGACAG CAGTCCACAG CTACTCTTAC CCCCTCACCT 
CCTGACCCTC CCCAGCCCCC CACTGACATC CCCCATCTCC ACCAGATGCC CAGGAGGGTG 
CCCCTGGGGC CAAGTTCCCC TCTCAGTGTA TCCCAGCCCA CTCTGAGTAG CCATGAAGGG 
AGGCCTGCTG GCCTGGGTGC TGGCCCCACA GCCTCCCATC ACCTCAACCC CAGTCCTGTC 
CCTAGTACAG CCAGCAGTGC CCCAGGGAGA ACCCGGCAGG TGCCTGGGGA GATGACCCCT 
CCGCTTCAAG GGCCCCGGGC CCGAATCCGG AAGCCCAAGG CTGTTCCCTA CCGGCGGGAG 
CACAGTCCTG GCGGTCTCTC CCCAGATTTG CCCCCACCAC CCTTGCCGCC ACCAGAGGAA 
GAGACGAGCT GGGCCTTAGG GCTGAGAGCA GCAGGCAGCA TGTCCTCCCT GGAACGAGAG 
CGGGAGCGCA GTGGGGAGAG AATGGTGCAG GCTGGGCTCC TGGGGGCCCA GCGGGGTCCC 
CACCTGGATG AAGAGGCCTG GCTCCCTTAC AGCCGACCGA GCTTCCTGTC CCGGGGCCAG 
GGGCCCAACA CCTGTTCCAC AGCCGGCAGC AACTCTTCGA GAGGCTCTGG CAGCTCTCGA 
GGCTCCCGGG GACCTGGACG GAGCCGGAGC CGGAGCCGGA GCCAGAGCCA GAGGCCAGGA 
CAGAAATGTG GAGAGGTGCG GGCTAGGGAA CCAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO3 ( XM_014113328.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968803.1 Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X2, mRNA.

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CloneID	OCe36282
Clone ID Related Accession (Same CDS sequence)	XM_014113328.1 , XM_014113328.2
Accession Version	XM_014113328.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	roundabout homolog 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113328.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGCT ACCTGCTGAA GACACTGCTG CAGATGAACT TGTTCGCGGA CTCCCTGGCG 
GGGGACATCT CCAACTCCAG CGACCTGCTC TTCGGCTTCA ACTCCTCGGT GGCGGCGCTC 
AACCACAGCC TGCTGCCCCC CGGCGACCCC TCTTTCAACG CGTCAAGGGT CGAGCCGGAG 
GACGCGATGC CGCGCATCGT GGAGCAGCCG CCAGATCTGC TGGTGTCCCG GGGCGAGCCG 
GCCACGCTGC CCTGCCGCGC CGAGGGCCGG CCCCGGCCCA ACATCGAGTG GTACAAGAAC 
GGGGCGCGGG TGGCCACTGC GCGCGAGGAC CCGCGCGCGC ACCGCCTGCT GCTGCCCAGC 
GGCGCCCTCT TCTTCCCCCG CATCGTGCAC GGGCGCCGCG CGCGCCCCGA CGAGGGTGTC 
TACACTTGCG TGGCGCGCAA CTACCTGGGG GCAGCGGCCA GCAGAAATGC CTCGCTGGAA 
GTGGCGGTCC TCCGTGATGA TTTCCGGCAG TCTCCTGGAA ACGTGGTGGT GGCAGTGGGC 
GAGCCAGCGG TGATGGAATG CGTTCCCCCC CGTGGCCACC CGGAGCCTTC CGTGTCCTGG 
AAGAAGGACA GTGCAAGACT CAGAGAGGAG GAGGGAAGGA TCACGATTCG TGGAGGGAAG 
CTGATGATGT CACATACACT CAAGAGTGAT GCGGGGATGT ATGTGTGCGT GGCCTCCAAC 
ATGGCAGGAG AACGGGAGAG TGGGGCAGCT GAACTTGTGG TACTGGAGCG ACCCTCATTC 
CTGCGTAGAC CAGTCAACCA GGTGGTCCTG GCAGATGCCC CCGTGGATTT CCCATGTGAG 
GTGCAGGGAG ATCCCCCACC CCGTCTACGC TGGCGCAAGG AGGATGGGGA ACTGCCCACC 
GGCAGGTATG AGATCCGGAG TGACCACAGC CTTTGGATCG GCCGCGTGAC TGCCGCCGAC 
GAGGGGACCT ACACCTGTGT GGCTGAGAAC AGCGTGGGCC GCGCCGAAGC ATCTGGCTCC 
CTCAGTGTTC ACGTCCCACC CCAGCTGGTG ACCCAGCCGC AGGACCAGAT GGCAGCTCCT 
GGAGAGAGTG TGGCTTTCCA ATGCGAGACC AAAGGAAACC CCCCACCTGC CATCTTCTGG 
CAGAAGGAGG GAAGTCAGGT TCTACTTTTC CCCAGCCAGC CGCTTCAGCC CAAGGGGCGC 
TTCTCTGTGT CTCCCAGAGG CCAGCTCAAC ATTACGGATG TGCAGAGCGG GGATGCTGGC 
TACTACGTGT GTCAGGCCGT TAGTGTGGCC GGGAGCGTCC TGGCTAAGGC CCTGCTGGAG 
GTAAAAAGAG CCTCCTCCTT GGATGGGCTG CCTCCTATCA TCCTCCAGGG ACCAGCCAAT 
CAGACGCTGG CACTTGGCTC CCCTGTGTGG CTGCCATGCA GAGTGACCGG GAACCCTCAA 
CCGAGTGTCC GATGGAAGAA GGATGGGCAG TGGCTGCAGG GGGACGACCT CCAGCTCAAC 
CTAATGGCCA ATGGTACTCT GTACATCGCC AGCGTGCAGG AGATGGACCT GGGTTTCTAC 
AGCTGTGTGG CCAAGAGTTC CATGGGGGAG GCCACATGGA GCAGCTGGCT TAGGAGGCGG 
GATTGGGGAG TATCACCAGA GCCCCCTACA GAACCCAGTA CCCCTCCAGG GCCTCCCTCT 
CAGCCAGTGG TCACTGAGAT CACCAAGAAC AGCATTACCC TGACCTGGAA GCCCAACCCG 
CAGGCGGGGG CCACAGTCAC CTCTTATGTG ATAGAGGCCT TCAGCCAAGC AGCTGGCAAC 
ACGTGGCGGA CAGTGGCAGA TGGTGTGCAG CTGGAGACAC ACACGGTCAG TGGTCTGCAG 
CCCAACACCA TCTATCTGTT TCTGGTGCGA GCTGTGGGAG CCTGGGGCCT CAGTGAGCCC 
AGCCCCGTCT CTGAGCCCAT CCGCACCCAG GACAGCAACC CATCCAGGCC AGTGGAGGAC 
CCATGGAGAG GCCAGCGGGG ACTGGCTGAA GTGGCTGTGC ATGTGCAGGA GCCCGTAGTC 
CTTGGGCTCC GGACCTTGCA GGTGTCCTGG ACTGTAGATG GCCCAGTCCA GCTGGTGCAA 
GGTTTCCGGG TGTCTTGGAG GGTAGCAGGT CCTGATGGGG GAAGCTGGAC AGTACTGGAC 
CTACAGTCCC CAAGCCAGCA AAGTACTGTG CTAAGAGGAC TCCCCCCAGG GACCCAAATT 
CAGATCAAAG TGCAAGCCCA AGGCCAGGAG GGGCTGGGGC CTGAAAGTCC CTTTGTGACC 
AGGAGCATTC CGGAGGAAGC CCCCAGTGGC CCCCCCCAGG GAGTGACAGT GGCCTTGGGG 
GGTGAAGGCA ACAGCAGTGT CACGGTGTCC TGGGAACCTC CCCTCCCCTC CCAGCAAAAT 
GGGGTGATCA AGGAATACCA GATCTGGTGC CTGGGCAATG AGAGCCGCTT CCACCTCAAC 
CGGTCTGCGG CAGGCTGGGC ACGCTCCGCC GTGCTCCGGG GACTGCTGCC CGGCCTTCTC 
TACCGGACCC GGGTCGCGGC GGCCACCAGC GCCGGCGTGG GCGTGGCCAG CGTCCCGGTG 
TCGGTGCAGC TGCCCTCCCC GCCGGAGTCG GAGCCCGGGC TCGAGGTGCG CCCGGGGCTG 
GCGGAGCGCC TGGCCAGGGT GCTGCGGGCG CCTGCCTTCC TGGCGGGCAG CGGGGCCGCC 
TGCGGGGCGC TGCTGCTCGC GCTCTGCGCC GTCCTCTACC GGCGCCGGAG GCAGCGCAAG 
GAGCTCAGTC ACTACACGGC CTCCTTTGCC TACACACCGG CAGTGTCCTT CCCACACTCA 
GAGGGCCTCT CTGGAGCCAG TTCCAGGCCA CCCATGGGCC TCGGCCCCGC CCCCTACCCT 
TGGCTGGCCG ACTCGTGGCC CCACCCATCT CGAAGCCCCT CAGCCCCGGA GCCCAGGGGG 
AGCTGCTGCC CCAGCAATCC TGACCCAGAC GACAGATATT ACAATGAAGC AGGGATCTCC 
CTGTACCTGG CTCAGACGGC CCGAGGCACT GCCGCCCCTA CAGAGGGTCC TGTCTACAGC 
ACCATTGACC CAGCTGGGGA GGAGCTGCAG ACCTTCCACG GGGGGTTCCC CCCAAATCCC 
TCGGGGGACC CAGGCGCCTG GAGCCAATAT GCTCCTCCAG AATGGAGCCA GGGGGACAGT 
GGAGCCAGGG GAGGCAAAGT AAAGCTTCTG GGAAAAGCTG TGCAGATGCC CTCTCTCAAC 
TGGCCGGAAG CCCTGCCACC ACCTCCCCCT TCCTGTGAAC TGAGCTGCCT AGAGGGGCCT 
GAGGAAGATC TGGAAGGCAG CTCAGAGCCA GAAGAGTGGT GCCCACCAAT GTCTGAGAGG 
AGCCATCTGA CAGAGCCCAG CTCCACTGGA GGGTGCTTGG TCCCTCCATC CCAAGGGGAA 
GCCCCCTCTC CCACATCTTC CTATGGACAG CAGTCCACAG CTACTCTTAC CCCCTCACCT 
CCTGACCCTC CCCAGCCCCC CACTGACATC CCCCATCTCC ACCAGATGCC CAGGAGGGTG 
CCCCTGGGGC CAAGTTCCCC TCTCAGTGTA TCCCAGCCCA CTCTGAGTAG CCATGAAGGG 
AGGCCTGCTG GCCTGGGTGC TGGCCCCACA GCCTCCCATC ACCTCAACCC CAGTCCTGTC 
CCTAGTACAG CCAGCAGTGC CCCAGGGAGA ACCCGGCAGG TGCCTGGGGA GATGACCCCT 
CCGCTTCAAG GGCCCCGGGC CCGAATCCGG AAGCCCAAGG CTGTTCCCTA CCGGCGGGAG 
CACAGTCCTG GCGGTCTCTC CCCAGATTTG CCCCCACCAC CCTTGCCGCC ACCAGAGGAA 
GAGACGAGCT GGGCCTTAGG GCTGAGAGCA GCAGGCAGCA TGTCCTCCCT GGAACGAGAG 
CGGGAGCGCA GTGGGGAGAG AATGGTGCAG GCTGGGCTCC TGGGGGCCCA GCGGGGTCCC 
CACCTGGATG AAGAGGCCTG GCTCCCTTAC AGCCGACCGA GCTTCCTGTC CCGGGGCCAG 
GGGCCCAACA CCTGTTCCAC AGCCGGCAGC AACTCTTCGA GAGGCTCTGG CAGCTCTCGA 
GGCTCCCGGG GACCTGGACG GAGCCGGAGC CGGAGCCGGA GCCAGAGCCA GAGGCCAGGA 
CAGAAATGTG GAGAGGTGCG GGCTAGGGAA CCAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968803.1
CDS	98..4276
Translation	MLRYLLKTLLQMNLFADSLAGDISNSSDLLFGFNSSVAALNHSLLPPGDPSFNASRVEPEDAMPRIVEQPPDLLVSRGEPATLPCRAEGRPRPNIEWYKNGARVATAREDPRAHRLLLPSGALFFPRIVHGRRARPDEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVMECVPPRGHPEPSVSWKKDSARLREEEGRITIRGGKLMMSHTLKSDAGMYVCVASNMAGERESGAAELVVLERPSFLRRPVNQVVLADAPVDFPCEVQGDPPPRLRWRKEDGELPTGRYEIRSDHSLWIGRVTAADEGTYTCVAENSVGRAEASGSLSVHVPPQLVTQPQDQMAAPGESVAFQCETKGNPPPAIFWQKEGSQVLLFPSQPLQPKGRFSVSPRGQLNITDVQSGDAGYYVCQAVSVAGSVLAKALLEVKRASSLDGLPPIILQGPANQTLALGSPVWLPCRVTGNPQPSVRWKKDGQWLQGDDLQLNLMANGTLYIASVQEMDLGFYSCVAKSSMGEATWSSWLRRRDWGVSPEPPTEPSTPPGPPSQPVVTEITKNSITLTWKPNPQAGATVTSYVIEAFSQAAGNTWRTVADGVQLETHTVSGLQPNTIYLFLVRAVGAWGLSEPSPVSEPIRTQDSNPSRPVEDPWRGQRGLAEVAVHVQEPVVLGLRTLQVSWTVDGPVQLVQGFRVSWRVAGPDGGSWTVLDLQSPSQQSTVLRGLPPGTQIQIKVQAQGQEGLGPESPFVTRSIPEEAPSGPPQGVTVALGGEGNSSVTVSWEPPLPSQQNGVIKEYQIWCLGNESRFHLNRSAAGWARSAVLRGLLPGLLYRTRVAAATSAGVGVASVPVSVQLPSPPESEPGLEVRPGLAERLARVLRAPAFLAGSGAACGALLLALCAVLYRRRRQRKELSHYTASFAYTPAVSFPHSEGLSGASSRPPMGLGPAPYPWLADSWPHPSRSPSAPEPRGSCCPSNPDPDDRYYNEAGISLYLAQTARGTAAPTEGPVYSTIDPAGEELQTFHGGFPPNPSGDPGAWSQYAPPEWSQGDSGARGGKVKLLGKAVQMPSLNWPEALPPPPPSCELSCLEGPEEDLEGSSEPEEWCPPMSERSHLTEPSSTGGCLVPPSQGEAPSPTSSYGQQSTATLTPSPPDPPQPPTDIPHLHQMPRRVPLGPSSPLSVSQPTLSSHEGRPAGLGAGPTASHHLNPSPVPSTASSAPGRTRQVPGEMTPPLQGPRARIRKPKAVPYRREHSPGGLSPDLPPPPLPPPEEETSWALGLRAAGSMSSLERERERSGERMVQAGLLGAQRGPHLDEEAWLPYSRPSFLSRGQGPNTCSTAGSNSSRGSGSSRGSRGPGRSRSRSRSQSQRPGQKCGEVRAREPR

Target ORF information:

RefSeq Version	XM_014113328.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113328.2

ORF Insert Sequence:

ATGCTGCGCT ACCTGCTGAA GACACTGCTG CAGATGAACT TGTTCGCGGA CTCCCTGGCG 
GGGGACATCT CCAACTCCAG CGACCTGCTC TTCGGCTTCA ACTCCTCGGT GGCGGCGCTC 
AACCACAGCC TGCTGCCCCC CGGCGACCCC TCTTTCAACG CGTCAAGGGT CGAGCCGGAG 
GACGCGATGC CGCGCATCGT GGAGCAGCCG CCAGATCTGC TGGTGTCCCG GGGCGAGCCG 
GCCACGCTGC CCTGCCGCGC CGAGGGCCGG CCCCGGCCCA ACATCGAGTG GTACAAGAAC 
GGGGCGCGGG TGGCCACTGC GCGCGAGGAC CCGCGCGCGC ACCGCCTGCT GCTGCCCAGC 
GGCGCCCTCT TCTTCCCCCG CATCGTGCAC GGGCGCCGCG CGCGCCCCGA CGAGGGTGTC 
TACACTTGCG TGGCGCGCAA CTACCTGGGG GCAGCGGCCA GCAGAAATGC CTCGCTGGAA 
GTGGCGGTCC TCCGTGATGA TTTCCGGCAG TCTCCTGGAA ACGTGGTGGT GGCAGTGGGC 
GAGCCAGCGG TGATGGAATG CGTTCCCCCC CGTGGCCACC CGGAGCCTTC CGTGTCCTGG 
AAGAAGGACA GTGCAAGACT CAGAGAGGAG GAGGGAAGGA TCACGATTCG TGGAGGGAAG 
CTGATGATGT CACATACACT CAAGAGTGAT GCGGGGATGT ATGTGTGCGT GGCCTCCAAC 
ATGGCAGGAG AACGGGAGAG TGGGGCAGCT GAACTTGTGG TACTGGAGCG ACCCTCATTC 
CTGCGTAGAC CAGTCAACCA GGTGGTCCTG GCAGATGCCC CCGTGGATTT CCCATGTGAG 
GTGCAGGGAG ATCCCCCACC CCGTCTACGC TGGCGCAAGG AGGATGGGGA ACTGCCCACC 
GGCAGGTATG AGATCCGGAG TGACCACAGC CTTTGGATCG GCCGCGTGAC TGCCGCCGAC 
GAGGGGACCT ACACCTGTGT GGCTGAGAAC AGCGTGGGCC GCGCCGAAGC ATCTGGCTCC 
CTCAGTGTTC ACGTCCCACC CCAGCTGGTG ACCCAGCCGC AGGACCAGAT GGCAGCTCCT 
GGAGAGAGTG TGGCTTTCCA ATGCGAGACC AAAGGAAACC CCCCACCTGC CATCTTCTGG 
CAGAAGGAGG GAAGTCAGGT TCTACTTTTC CCCAGCCAGC CGCTTCAGCC CAAGGGGCGC 
TTCTCTGTGT CTCCCAGAGG CCAGCTCAAC ATTACGGATG TGCAGAGCGG GGATGCTGGC 
TACTACGTGT GTCAGGCCGT TAGTGTGGCC GGGAGCGTCC TGGCTAAGGC CCTGCTGGAG 
GTAAAAAGAG CCTCCTCCTT GGATGGGCTG CCTCCTATCA TCCTCCAGGG ACCAGCCAAT 
CAGACGCTGG CACTTGGCTC CCCTGTGTGG CTGCCATGCA GAGTGACCGG GAACCCTCAA 
CCGAGTGTCC GATGGAAGAA GGATGGGCAG TGGCTGCAGG GGGACGACCT CCAGCTCAAC 
CTAATGGCCA ATGGTACTCT GTACATCGCC AGCGTGCAGG AGATGGACCT GGGTTTCTAC 
AGCTGTGTGG CCAAGAGTTC CATGGGGGAG GCCACATGGA GCAGCTGGCT TAGGAGGCGG 
GATTGGGGAG TATCACCAGA GCCCCCTACA GAACCCAGTA CCCCTCCAGG GCCTCCCTCT 
CAGCCAGTGG TCACTGAGAT CACCAAGAAC AGCATTACCC TGACCTGGAA GCCCAACCCG 
CAGGCGGGGG CCACAGTCAC CTCTTATGTG ATAGAGGCCT TCAGCCAAGC AGCTGGCAAC 
ACGTGGCGGA CAGTGGCAGA TGGTGTGCAG CTGGAGACAC ACACGGTCAG TGGTCTGCAG 
CCCAACACCA TCTATCTGTT TCTGGTGCGA GCTGTGGGAG CCTGGGGCCT CAGTGAGCCC 
AGCCCCGTCT CTGAGCCCAT CCGCACCCAG GACAGCAACC CATCCAGGCC AGTGGAGGAC 
CCATGGAGAG GCCAGCGGGG ACTGGCTGAA GTGGCTGTGC ATGTGCAGGA GCCCGTAGTC 
CTTGGGCTCC GGACCTTGCA GGTGTCCTGG ACTGTAGATG GCCCAGTCCA GCTGGTGCAA 
GGTTTCCGGG TGTCTTGGAG GGTAGCAGGT CCTGATGGGG GAAGCTGGAC AGTACTGGAC 
CTACAGTCCC CAAGCCAGCA AAGTACTGTG CTAAGAGGAC TCCCCCCAGG GACCCAAATT 
CAGATCAAAG TGCAAGCCCA AGGCCAGGAG GGGCTGGGGC CTGAAAGTCC CTTTGTGACC 
AGGAGCATTC CGGAGGAAGC CCCCAGTGGC CCCCCCCAGG GAGTGACAGT GGCCTTGGGG 
GGTGAAGGCA ACAGCAGTGT CACGGTGTCC TGGGAACCTC CCCTCCCCTC CCAGCAAAAT 
GGGGTGATCA AGGAATACCA GATCTGGTGC CTGGGCAATG AGAGCCGCTT CCACCTCAAC 
CGGTCTGCGG CAGGCTGGGC ACGCTCCGCC GTGCTCCGGG GACTGCTGCC CGGCCTTCTC 
TACCGGACCC GGGTCGCGGC GGCCACCAGC GCCGGCGTGG GCGTGGCCAG CGTCCCGGTG 
TCGGTGCAGC TGCCCTCCCC GCCGGAGTCG GAGCCCGGGC TCGAGGTGCG CCCGGGGCTG 
GCGGAGCGCC TGGCCAGGGT GCTGCGGGCG CCTGCCTTCC TGGCGGGCAG CGGGGCCGCC 
TGCGGGGCGC TGCTGCTCGC GCTCTGCGCC GTCCTCTACC GGCGCCGGAG GCAGCGCAAG 
GAGCTCAGTC ACTACACGGC CTCCTTTGCC TACACACCGG CAGTGTCCTT CCCACACTCA 
GAGGGCCTCT CTGGAGCCAG TTCCAGGCCA CCCATGGGCC TCGGCCCCGC CCCCTACCCT 
TGGCTGGCCG ACTCGTGGCC CCACCCATCT CGAAGCCCCT CAGCCCCGGA GCCCAGGGGG 
AGCTGCTGCC CCAGCAATCC TGACCCAGAC GACAGATATT ACAATGAAGC AGGGATCTCC 
CTGTACCTGG CTCAGACGGC CCGAGGCACT GCCGCCCCTA CAGAGGGTCC TGTCTACAGC 
ACCATTGACC CAGCTGGGGA GGAGCTGCAG ACCTTCCACG GGGGGTTCCC CCCAAATCCC 
TCGGGGGACC CAGGCGCCTG GAGCCAATAT GCTCCTCCAG AATGGAGCCA GGGGGACAGT 
GGAGCCAGGG GAGGCAAAGT AAAGCTTCTG GGAAAAGCTG TGCAGATGCC CTCTCTCAAC 
TGGCCGGAAG CCCTGCCACC ACCTCCCCCT TCCTGTGAAC TGAGCTGCCT AGAGGGGCCT 
GAGGAAGATC TGGAAGGCAG CTCAGAGCCA GAAGAGTGGT GCCCACCAAT GTCTGAGAGG 
AGCCATCTGA CAGAGCCCAG CTCCACTGGA GGGTGCTTGG TCCCTCCATC CCAAGGGGAA 
GCCCCCTCTC CCACATCTTC CTATGGACAG CAGTCCACAG CTACTCTTAC CCCCTCACCT 
CCTGACCCTC CCCAGCCCCC CACTGACATC CCCCATCTCC ACCAGATGCC CAGGAGGGTG 
CCCCTGGGGC CAAGTTCCCC TCTCAGTGTA TCCCAGCCCA CTCTGAGTAG CCATGAAGGG 
AGGCCTGCTG GCCTGGGTGC TGGCCCCACA GCCTCCCATC ACCTCAACCC CAGTCCTGTC 
CCTAGTACAG CCAGCAGTGC CCCAGGGAGA ACCCGGCAGG TGCCTGGGGA GATGACCCCT 
CCGCTTCAAG GGCCCCGGGC CCGAATCCGG AAGCCCAAGG CTGTTCCCTA CCGGCGGGAG 
CACAGTCCTG GCGGTCTCTC CCCAGATTTG CCCCCACCAC CCTTGCCGCC ACCAGAGGAA 
GAGACGAGCT GGGCCTTAGG GCTGAGAGCA GCAGGCAGCA TGTCCTCCCT GGAACGAGAG 
CGGGAGCGCA GTGGGGAGAG AATGGTGCAG GCTGGGCTCC TGGGGGCCCA GCGGGGTCCC 
CACCTGGATG AAGAGGCCTG GCTCCCTTAC AGCCGACCGA GCTTCCTGTC CCGGGGCCAG 
GGGCCCAACA CCTGTTCCAC AGCCGGCAGC AACTCTTCGA GAGGCTCTGG CAGCTCTCGA 
GGCTCCCGGG GACCTGGACG GAGCCGGAGC CGGAGCCGGA GCCAGAGCCA GAGGCCAGGA 
CAGAAATGTG GAGAGGTGCG GGCTAGGGAA CCAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO3 ( XM_014113329.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968804.1 Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe36283
Clone ID Related Accession (Same CDS sequence)	XM_014113329.1 , XM_014113329.2
Accession Version	XM_014113329.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	roundabout homolog 3 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGCT ACCTGCTGAA GACACTGCTG CAGATGAACT TGTTCGCGGA CTCCCTGGCG 
GGGGACATCT CCAACTCCAG CGACCTGCTC TTCGGCTTCA ACTCCTCGGT GGCGGCGCTC 
AACCACAGCC TGCTGCCCCC CGGCGACCCC TCTTTCAACG CGTCAAGGGT CGAGCCGGAG 
GACGCGATGC CGCGCATCGT GGAGCAGCCG CCAGATCTGC TGGTGTCCCG GGGCGAGCCG 
GCCACGCTGC CCTGCCGCGC CGAGGGCCGG CCCCGGCCCA ACATCGAGTG GTACAAGAAC 
GGGGCGCGGG TGGCCACTGC GCGCGAGGAC CCGCGCGCGC ACCGCCTGCT GCTGCCCAGC 
GGCGCCCTCT TCTTCCCCCG CATCGTGCAC GGGCGCCGCG CGCGCCCCGA CGAGGGTGTC 
TACACTTGCG TGGCGCGCAA CTACCTGGGG GCAGCGGCCA GCAGAAATGC CTCGCTGGAA 
GTGGCGGTCC TCCGTGATGA TTTCCGGCAG TCTCCTGGAA ACGTGGTGGT GGCAGTGGGC 
GAGCCAGCGG TGATGGAATG CGTTCCCCCC CGTGGCCACC CGGAGCCTTC CGTGTCCTGG 
AAGAAGGACA GTGCAAGACT CAGAGAGGAG GAGGGAAGGA TCACGATTCG TGGAGGGAAG 
CTGATGATGT CACATACACT CAAGAGTGAT GCGGGGATGT ATGTGTGCGT GGCCTCCAAC 
ATGGCAGGAG AACGGGAGAG TGGGGCAGCT GAACTTGTGG TACTGGAGCG ACCCTCATTC 
CTGCGTAGAC CAGTCAACCA GGTGGTCCTG GCAGATGCCC CCGTGGATTT CCCATGTGAG 
GTGCAGGGAG ATCCCCCACC CCGTCTACGC TGGCGCAAGG AGGATGGGGA ACTGCCCACC 
GGCAGGTATG AGATCCGGAG TGACCACAGC CTTTGGATCG GCCGCGTGAC TGCCGCCGAC 
GAGGGGACCT ACACCTGTGT GGCTGAGAAC AGCGTGGGCC GCGCCGAAGC ATCTGGCTCC 
CTCAGTGTTC ACGTCCCACC CCAGCTGGTG ACCCAGCCGC AGGACCAGAT GGCAGCTCCT 
GGAGAGAGTG TGGCTTTCCA ATGCGAGACC AAAGGAAACC CCCCACCTGC CATCTTCTGG 
CAGAAGGAGG GAAGTCAGGT TCTACTTTTC CCCAGCCAGC CGCTTCAGCC CAAGGGGCGC 
TTCTCTGTGT CTCCCAGAGG CCAGCTCAAC ATTACGGATG TGCAGAGCGG GGATGCTGGC 
TACTACGTGT GTCAGGCCGT TAGTGTGGCC GGGAGCGTCC TGGCTAAGGC CCTGCTGGAG 
GTAAAAAGAG CCTCCTCCTT GGATGGGCTG CCTCCTATCA TCCTCCAGGG ACCAGCCAAT 
CAGACGCTGG CACTTGGCTC CCCTGTGTGG CTGCCATGCA GAGTGACCGG GAACCCTCAA 
CCGAGTGTCC GATGGAAGAA GGATGGGCAG TGGCTGCAGG GGGACGACCT CCAGCTCAAC 
CTAATGGCCA ATGGTACTCT GTACATCGCC AGCGTGCAGG AGATGGACCT GGGTTTCTAC 
AGCTGTGTGG CCAAGAGTTC CATGGGGGAG GCCACATGGA GCAGCTGGCT TAGGAGGCGG 
GAAGATTGGG GAGTATCACC AGAGCCCCCT ACAGAACCCA GTACCCCTCC AGGGCCTCCC 
TCTCAGCCAG TGGTCACTGA GATCACCAAG AACAGCATTA CCCTGACCTG GAAGCCCAAC 
CCGCAGGCGG GGGCCACAGT CACCTCTTAT GTGATAGAGG CCTTCAGCCA AGCAGCTGGC 
AACACGTGGC GGACAGTGGC AGATGGTGTG CAGCTGGAGA CACACACGGT CAGTGGTCTG 
CAGCCCAACA CCATCTATCT GTTTCTGGTG CGAGCTGTGG GAGCCTGGGG CCTCAGTGAG 
CCCAGCCCCG TCTCTGAGCC CATCCGCACC CAGGACAGCA ACCCATCCAG GCCAGTGGAG 
GACCCATGGA GAGGCCAGCG GGGACTGGCT GAAGTGGCTG TGCATGTGCA GGAGCCCGTA 
GTCCTTGGGC TCCGGACCTT GCAGGTGTCC TGGACTGTAG ATGGCCCAGT CCAGCTGGTG 
CAAGGTTTCC GGGTGTCTTG GAGGGTAGCA GGTCCTGATG GGGGAAGCTG GACAGTACTG 
GACCTACAGT CCCCAAGCCA GCAAAGTACT GTGCTAAGAG GACTCCCCCC AGGGACCCAA 
ATTCAGATCA AAGTGCAAGC CCAAGGCCAG GAGGGGCTGG GGCCTGAAAG TCCCTTTGTG 
ACCAGGAGCA TTCCGGAGGA AGCCCCCAGT GGCCCCCCCC AGGGAGTGAC AGTGGCCTTG 
GGGGGTGAAG GCAACAGCAG TGTCACGGTG TCCTGGGAAC CTCCCCTCCC CTCCCAGCAA 
AATGGGGTGA TCAAGGAATA CCAGATCTGG TGCCTGGGCA ATGAGAGCCG CTTCCACCTC 
AACCGGTCTG CGGCAGGCTG GGCACGCTCC GCCGTGCTCC GGGGACTGCT GCCCGGCCTT 
CTCTACCGGA CCCGGGTCGC GGCGGCCACC AGCGCCGGCG TGGGCGTGGC CAGCGTCCCG 
GTGTCGGTGC AGCTGCCCTC CCCGCCGGAG TCGGAGCCCG GGCTCGAGGT GCGCCCGGGG 
CTGGCGGAGC GCCTGGCCAG GGTGCTGCGG GCGCCTGCCT TCCTGGCGGG CAGCGGGGCC 
GCCTGCGGGG CGCTGCTGCT CGCGCTCTGC GCCGTCCTCT ACCGGCGCCG GAGGCAGCGC 
AAGGAGCTCA GTCACTACAC GGCCTCCTTT GCCTACACAC CGGCAGTGTC CTTCCCACAC 
TCAGAGGGCC TCTCTGGAGC CAGTTCCAGG CCACCCATGG GCCTCGGCCC CGCCCCCTAC 
CCTTGGCTGG CCGACTCGTG GCCCCACCCA TCTCGAAGCC CCTCAGCCCC GGAGCCCAGG 
GGGAGCTGCT GCCCCAGCAA TCCTGACCCA GACGACAGAT ATTACAATGA AGCAGGGATC 
TCCCTGTACC TGGCTCAGAC GGCCCGAGGC ACTGCCGCCC CTACAGAGGG TCCTGTCTAC 
AGCACCATTG ACCCAGCTGG GGAGGAGCTG CAGACCTTCC ACGGGGGGTT CCCCCCAAAT 
CCCTCGGGGG ACCCAGGCGC CTGGAGCCAA TATGCTCCTC CAGAATGGAG CCAGGGGGAC 
AGTGGAGCCA GGGGAGGCAA AGTAAAGCTT CTGGGAAAAG CTGTGCAGAT GCCCTCTCTC 
AACTGGCCGG AAGCCCTGCC ACCACCTCCC CCTTCCTGTG AACTGAGCTG CCTAGAGGGG 
CCTGAGGAAG ATCTGGAAGG CAGCTCAGAG CCAGAAGAGT GGTGCCCACC AATGTCTGAG 
AGGAGCCATC TGACAGAGCC CAGCTCCACT GGAGGGTGCT TGGTCCCTCC ATCCCAAGGG 
GAAGCCCCCT CTCCCACATC TTCCTATGGA CAGCAGTCCA CAGCTACTCT TACCCCCTCA 
CCTCCTGACC CTCCCCAGCC CCCCACTGAC ATCCCCCATC TCCACCAGAT GCCCAGGAGG 
GTGCCCCTGG GGCCAAGTTC CCCTCTCAGT GTATCCCAGC CCACTCTGAG TAGCCATGAA 
GGGAGGCCTG CTGGCCTGGG TGCTGGCCCC ACAGCCTCCC ATCACCTCAA CCCCAGTCCT 
GTCCCTAGTA CAGCCAGCAG TGCCCCAGGG AGAACCCGGC AGGTGCCTGG GGAGATGACC 
CCTCCGCTTC AAGGGCCCCG GGCCCGAATC CGGAAGCCCA AGGCTGTTCC CTACCGGCGG 
GAGCACAGTC CTGGCGATTT GCCCCCACCA CCCTTGCCGC CACCAGAGGA AGAGACGAGC 
TGGGCCTTAG GGCTGAGAGC AGCAGGCAGC ATGTCCTCCC TGGAACGAGA GCGGGAGCGC 
AGTGGGGAGA GAATGGTGCA GGCTGGGCTC CTGGGGGCCC AGCGGGGTCC CCACCTGGAT 
GAAGAGGCCT GGCTCCCTTA CAGCCGACCG AGCTTCCTGT CCCGGGGCCA GGGGCCCAAC 
ACCTGTTCCA CAGCCGGCAG CAACTCTTCG AGAGGCTCTG GCAGCTCTCG AGGCTCCCGG 
GGACCTGGAC GGAGCCGGAG CCGGAGCCGG AGCCAGAGCC AGAGGCCAGG ACAGAAATGT 
GGAGAGGTGC GGGCTAGGGA ACCAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968804.1
CDS	193..4362
Translation	MLRYLLKTLLQMNLFADSLAGDISNSSDLLFGFNSSVAALNHSLLPPGDPSFNASRVEPEDAMPRIVEQPPDLLVSRGEPATLPCRAEGRPRPNIEWYKNGARVATAREDPRAHRLLLPSGALFFPRIVHGRRARPDEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVMECVPPRGHPEPSVSWKKDSARLREEEGRITIRGGKLMMSHTLKSDAGMYVCVASNMAGERESGAAELVVLERPSFLRRPVNQVVLADAPVDFPCEVQGDPPPRLRWRKEDGELPTGRYEIRSDHSLWIGRVTAADEGTYTCVAENSVGRAEASGSLSVHVPPQLVTQPQDQMAAPGESVAFQCETKGNPPPAIFWQKEGSQVLLFPSQPLQPKGRFSVSPRGQLNITDVQSGDAGYYVCQAVSVAGSVLAKALLEVKRASSLDGLPPIILQGPANQTLALGSPVWLPCRVTGNPQPSVRWKKDGQWLQGDDLQLNLMANGTLYIASVQEMDLGFYSCVAKSSMGEATWSSWLRRREDWGVSPEPPTEPSTPPGPPSQPVVTEITKNSITLTWKPNPQAGATVTSYVIEAFSQAAGNTWRTVADGVQLETHTVSGLQPNTIYLFLVRAVGAWGLSEPSPVSEPIRTQDSNPSRPVEDPWRGQRGLAEVAVHVQEPVVLGLRTLQVSWTVDGPVQLVQGFRVSWRVAGPDGGSWTVLDLQSPSQQSTVLRGLPPGTQIQIKVQAQGQEGLGPESPFVTRSIPEEAPSGPPQGVTVALGGEGNSSVTVSWEPPLPSQQNGVIKEYQIWCLGNESRFHLNRSAAGWARSAVLRGLLPGLLYRTRVAAATSAGVGVASVPVSVQLPSPPESEPGLEVRPGLAERLARVLRAPAFLAGSGAACGALLLALCAVLYRRRRQRKELSHYTASFAYTPAVSFPHSEGLSGASSRPPMGLGPAPYPWLADSWPHPSRSPSAPEPRGSCCPSNPDPDDRYYNEAGISLYLAQTARGTAAPTEGPVYSTIDPAGEELQTFHGGFPPNPSGDPGAWSQYAPPEWSQGDSGARGGKVKLLGKAVQMPSLNWPEALPPPPPSCELSCLEGPEEDLEGSSEPEEWCPPMSERSHLTEPSSTGGCLVPPSQGEAPSPTSSYGQQSTATLTPSPPDPPQPPTDIPHLHQMPRRVPLGPSSPLSVSQPTLSSHEGRPAGLGAGPTASHHLNPSPVPSTASSAPGRTRQVPGEMTPPLQGPRARIRKPKAVPYRREHSPGDLPPPPLPPPEEETSWALGLRAAGSMSSLERERERSGERMVQAGLLGAQRGPHLDEEAWLPYSRPSFLSRGQGPNTCSTAGSNSSRGSGSSRGSRGPGRSRSRSRSQSQRPGQKCGEVRAREPR

Target ORF information:

RefSeq Version	XM_014113329.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113329.1

ORF Insert Sequence:

ATGCTGCGCT ACCTGCTGAA GACACTGCTG CAGATGAACT TGTTCGCGGA CTCCCTGGCG 
GGGGACATCT CCAACTCCAG CGACCTGCTC TTCGGCTTCA ACTCCTCGGT GGCGGCGCTC 
AACCACAGCC TGCTGCCCCC CGGCGACCCC TCTTTCAACG CGTCAAGGGT CGAGCCGGAG 
GACGCGATGC CGCGCATCGT GGAGCAGCCG CCAGATCTGC TGGTGTCCCG GGGCGAGCCG 
GCCACGCTGC CCTGCCGCGC CGAGGGCCGG CCCCGGCCCA ACATCGAGTG GTACAAGAAC 
GGGGCGCGGG TGGCCACTGC GCGCGAGGAC CCGCGCGCGC ACCGCCTGCT GCTGCCCAGC 
GGCGCCCTCT TCTTCCCCCG CATCGTGCAC GGGCGCCGCG CGCGCCCCGA CGAGGGTGTC 
TACACTTGCG TGGCGCGCAA CTACCTGGGG GCAGCGGCCA GCAGAAATGC CTCGCTGGAA 
GTGGCGGTCC TCCGTGATGA TTTCCGGCAG TCTCCTGGAA ACGTGGTGGT GGCAGTGGGC 
GAGCCAGCGG TGATGGAATG CGTTCCCCCC CGTGGCCACC CGGAGCCTTC CGTGTCCTGG 
AAGAAGGACA GTGCAAGACT CAGAGAGGAG GAGGGAAGGA TCACGATTCG TGGAGGGAAG 
CTGATGATGT CACATACACT CAAGAGTGAT GCGGGGATGT ATGTGTGCGT GGCCTCCAAC 
ATGGCAGGAG AACGGGAGAG TGGGGCAGCT GAACTTGTGG TACTGGAGCG ACCCTCATTC 
CTGCGTAGAC CAGTCAACCA GGTGGTCCTG GCAGATGCCC CCGTGGATTT CCCATGTGAG 
GTGCAGGGAG ATCCCCCACC CCGTCTACGC TGGCGCAAGG AGGATGGGGA ACTGCCCACC 
GGCAGGTATG AGATCCGGAG TGACCACAGC CTTTGGATCG GCCGCGTGAC TGCCGCCGAC 
GAGGGGACCT ACACCTGTGT GGCTGAGAAC AGCGTGGGCC GCGCCGAAGC ATCTGGCTCC 
CTCAGTGTTC ACGTCCCACC CCAGCTGGTG ACCCAGCCGC AGGACCAGAT GGCAGCTCCT 
GGAGAGAGTG TGGCTTTCCA ATGCGAGACC AAAGGAAACC CCCCACCTGC CATCTTCTGG 
CAGAAGGAGG GAAGTCAGGT TCTACTTTTC CCCAGCCAGC CGCTTCAGCC CAAGGGGCGC 
TTCTCTGTGT CTCCCAGAGG CCAGCTCAAC ATTACGGATG TGCAGAGCGG GGATGCTGGC 
TACTACGTGT GTCAGGCCGT TAGTGTGGCC GGGAGCGTCC TGGCTAAGGC CCTGCTGGAG 
GTAAAAAGAG CCTCCTCCTT GGATGGGCTG CCTCCTATCA TCCTCCAGGG ACCAGCCAAT 
CAGACGCTGG CACTTGGCTC CCCTGTGTGG CTGCCATGCA GAGTGACCGG GAACCCTCAA 
CCGAGTGTCC GATGGAAGAA GGATGGGCAG TGGCTGCAGG GGGACGACCT CCAGCTCAAC 
CTAATGGCCA ATGGTACTCT GTACATCGCC AGCGTGCAGG AGATGGACCT GGGTTTCTAC 
AGCTGTGTGG CCAAGAGTTC CATGGGGGAG GCCACATGGA GCAGCTGGCT TAGGAGGCGG 
GAAGATTGGG GAGTATCACC AGAGCCCCCT ACAGAACCCA GTACCCCTCC AGGGCCTCCC 
TCTCAGCCAG TGGTCACTGA GATCACCAAG AACAGCATTA CCCTGACCTG GAAGCCCAAC 
CCGCAGGCGG GGGCCACAGT CACCTCTTAT GTGATAGAGG CCTTCAGCCA AGCAGCTGGC 
AACACGTGGC GGACAGTGGC AGATGGTGTG CAGCTGGAGA CACACACGGT CAGTGGTCTG 
CAGCCCAACA CCATCTATCT GTTTCTGGTG CGAGCTGTGG GAGCCTGGGG CCTCAGTGAG 
CCCAGCCCCG TCTCTGAGCC CATCCGCACC CAGGACAGCA ACCCATCCAG GCCAGTGGAG 
GACCCATGGA GAGGCCAGCG GGGACTGGCT GAAGTGGCTG TGCATGTGCA GGAGCCCGTA 
GTCCTTGGGC TCCGGACCTT GCAGGTGTCC TGGACTGTAG ATGGCCCAGT CCAGCTGGTG 
CAAGGTTTCC GGGTGTCTTG GAGGGTAGCA GGTCCTGATG GGGGAAGCTG GACAGTACTG 
GACCTACAGT CCCCAAGCCA GCAAAGTACT GTGCTAAGAG GACTCCCCCC AGGGACCCAA 
ATTCAGATCA AAGTGCAAGC CCAAGGCCAG GAGGGGCTGG GGCCTGAAAG TCCCTTTGTG 
ACCAGGAGCA TTCCGGAGGA AGCCCCCAGT GGCCCCCCCC AGGGAGTGAC AGTGGCCTTG 
GGGGGTGAAG GCAACAGCAG TGTCACGGTG TCCTGGGAAC CTCCCCTCCC CTCCCAGCAA 
AATGGGGTGA TCAAGGAATA CCAGATCTGG TGCCTGGGCA ATGAGAGCCG CTTCCACCTC 
AACCGGTCTG CGGCAGGCTG GGCACGCTCC GCCGTGCTCC GGGGACTGCT GCCCGGCCTT 
CTCTACCGGA CCCGGGTCGC GGCGGCCACC AGCGCCGGCG TGGGCGTGGC CAGCGTCCCG 
GTGTCGGTGC AGCTGCCCTC CCCGCCGGAG TCGGAGCCCG GGCTCGAGGT GCGCCCGGGG 
CTGGCGGAGC GCCTGGCCAG GGTGCTGCGG GCGCCTGCCT TCCTGGCGGG CAGCGGGGCC 
GCCTGCGGGG CGCTGCTGCT CGCGCTCTGC GCCGTCCTCT ACCGGCGCCG GAGGCAGCGC 
AAGGAGCTCA GTCACTACAC GGCCTCCTTT GCCTACACAC CGGCAGTGTC CTTCCCACAC 
TCAGAGGGCC TCTCTGGAGC CAGTTCCAGG CCACCCATGG GCCTCGGCCC CGCCCCCTAC 
CCTTGGCTGG CCGACTCGTG GCCCCACCCA TCTCGAAGCC CCTCAGCCCC GGAGCCCAGG 
GGGAGCTGCT GCCCCAGCAA TCCTGACCCA GACGACAGAT ATTACAATGA AGCAGGGATC 
TCCCTGTACC TGGCTCAGAC GGCCCGAGGC ACTGCCGCCC CTACAGAGGG TCCTGTCTAC 
AGCACCATTG ACCCAGCTGG GGAGGAGCTG CAGACCTTCC ACGGGGGGTT CCCCCCAAAT 
CCCTCGGGGG ACCCAGGCGC CTGGAGCCAA TATGCTCCTC CAGAATGGAG CCAGGGGGAC 
AGTGGAGCCA GGGGAGGCAA AGTAAAGCTT CTGGGAAAAG CTGTGCAGAT GCCCTCTCTC 
AACTGGCCGG AAGCCCTGCC ACCACCTCCC CCTTCCTGTG AACTGAGCTG CCTAGAGGGG 
CCTGAGGAAG ATCTGGAAGG CAGCTCAGAG CCAGAAGAGT GGTGCCCACC AATGTCTGAG 
AGGAGCCATC TGACAGAGCC CAGCTCCACT GGAGGGTGCT TGGTCCCTCC ATCCCAAGGG 
GAAGCCCCCT CTCCCACATC TTCCTATGGA CAGCAGTCCA CAGCTACTCT TACCCCCTCA 
CCTCCTGACC CTCCCCAGCC CCCCACTGAC ATCCCCCATC TCCACCAGAT GCCCAGGAGG 
GTGCCCCTGG GGCCAAGTTC CCCTCTCAGT GTATCCCAGC CCACTCTGAG TAGCCATGAA 
GGGAGGCCTG CTGGCCTGGG TGCTGGCCCC ACAGCCTCCC ATCACCTCAA CCCCAGTCCT 
GTCCCTAGTA CAGCCAGCAG TGCCCCAGGG AGAACCCGGC AGGTGCCTGG GGAGATGACC 
CCTCCGCTTC AAGGGCCCCG GGCCCGAATC CGGAAGCCCA AGGCTGTTCC CTACCGGCGG 
GAGCACAGTC CTGGCGATTT GCCCCCACCA CCCTTGCCGC CACCAGAGGA AGAGACGAGC 
TGGGCCTTAG GGCTGAGAGC AGCAGGCAGC ATGTCCTCCC TGGAACGAGA GCGGGAGCGC 
AGTGGGGAGA GAATGGTGCA GGCTGGGCTC CTGGGGGCCC AGCGGGGTCC CCACCTGGAT 
GAAGAGGCCT GGCTCCCTTA CAGCCGACCG AGCTTCCTGT CCCGGGGCCA GGGGCCCAAC 
ACCTGTTCCA CAGCCGGCAG CAACTCTTCG AGAGGCTCTG GCAGCTCTCG AGGCTCCCGG 
GGACCTGGAC GGAGCCGGAG CCGGAGCCGG AGCCAGAGCC AGAGGCCAGG ACAGAAATGT 
GGAGAGGTGC GGGCTAGGGA ACCAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO3 ( XM_014113329.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968804.1 Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe36283
Clone ID Related Accession (Same CDS sequence)	XM_014113329.1 , XM_014113329.2
Accession Version	XM_014113329.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	roundabout homolog 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113329.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGCT ACCTGCTGAA GACACTGCTG CAGATGAACT TGTTCGCGGA CTCCCTGGCG 
GGGGACATCT CCAACTCCAG CGACCTGCTC TTCGGCTTCA ACTCCTCGGT GGCGGCGCTC 
AACCACAGCC TGCTGCCCCC CGGCGACCCC TCTTTCAACG CGTCAAGGGT CGAGCCGGAG 
GACGCGATGC CGCGCATCGT GGAGCAGCCG CCAGATCTGC TGGTGTCCCG GGGCGAGCCG 
GCCACGCTGC CCTGCCGCGC CGAGGGCCGG CCCCGGCCCA ACATCGAGTG GTACAAGAAC 
GGGGCGCGGG TGGCCACTGC GCGCGAGGAC CCGCGCGCGC ACCGCCTGCT GCTGCCCAGC 
GGCGCCCTCT TCTTCCCCCG CATCGTGCAC GGGCGCCGCG CGCGCCCCGA CGAGGGTGTC 
TACACTTGCG TGGCGCGCAA CTACCTGGGG GCAGCGGCCA GCAGAAATGC CTCGCTGGAA 
GTGGCGGTCC TCCGTGATGA TTTCCGGCAG TCTCCTGGAA ACGTGGTGGT GGCAGTGGGC 
GAGCCAGCGG TGATGGAATG CGTTCCCCCC CGTGGCCACC CGGAGCCTTC CGTGTCCTGG 
AAGAAGGACA GTGCAAGACT CAGAGAGGAG GAGGGAAGGA TCACGATTCG TGGAGGGAAG 
CTGATGATGT CACATACACT CAAGAGTGAT GCGGGGATGT ATGTGTGCGT GGCCTCCAAC 
ATGGCAGGAG AACGGGAGAG TGGGGCAGCT GAACTTGTGG TACTGGAGCG ACCCTCATTC 
CTGCGTAGAC CAGTCAACCA GGTGGTCCTG GCAGATGCCC CCGTGGATTT CCCATGTGAG 
GTGCAGGGAG ATCCCCCACC CCGTCTACGC TGGCGCAAGG AGGATGGGGA ACTGCCCACC 
GGCAGGTATG AGATCCGGAG TGACCACAGC CTTTGGATCG GCCGCGTGAC TGCCGCCGAC 
GAGGGGACCT ACACCTGTGT GGCTGAGAAC AGCGTGGGCC GCGCCGAAGC ATCTGGCTCC 
CTCAGTGTTC ACGTCCCACC CCAGCTGGTG ACCCAGCCGC AGGACCAGAT GGCAGCTCCT 
GGAGAGAGTG TGGCTTTCCA ATGCGAGACC AAAGGAAACC CCCCACCTGC CATCTTCTGG 
CAGAAGGAGG GAAGTCAGGT TCTACTTTTC CCCAGCCAGC CGCTTCAGCC CAAGGGGCGC 
TTCTCTGTGT CTCCCAGAGG CCAGCTCAAC ATTACGGATG TGCAGAGCGG GGATGCTGGC 
TACTACGTGT GTCAGGCCGT TAGTGTGGCC GGGAGCGTCC TGGCTAAGGC CCTGCTGGAG 
GTAAAAAGAG CCTCCTCCTT GGATGGGCTG CCTCCTATCA TCCTCCAGGG ACCAGCCAAT 
CAGACGCTGG CACTTGGCTC CCCTGTGTGG CTGCCATGCA GAGTGACCGG GAACCCTCAA 
CCGAGTGTCC GATGGAAGAA GGATGGGCAG TGGCTGCAGG GGGACGACCT CCAGCTCAAC 
CTAATGGCCA ATGGTACTCT GTACATCGCC AGCGTGCAGG AGATGGACCT GGGTTTCTAC 
AGCTGTGTGG CCAAGAGTTC CATGGGGGAG GCCACATGGA GCAGCTGGCT TAGGAGGCGG 
GAAGATTGGG GAGTATCACC AGAGCCCCCT ACAGAACCCA GTACCCCTCC AGGGCCTCCC 
TCTCAGCCAG TGGTCACTGA GATCACCAAG AACAGCATTA CCCTGACCTG GAAGCCCAAC 
CCGCAGGCGG GGGCCACAGT CACCTCTTAT GTGATAGAGG CCTTCAGCCA AGCAGCTGGC 
AACACGTGGC GGACAGTGGC AGATGGTGTG CAGCTGGAGA CACACACGGT CAGTGGTCTG 
CAGCCCAACA CCATCTATCT GTTTCTGGTG CGAGCTGTGG GAGCCTGGGG CCTCAGTGAG 
CCCAGCCCCG TCTCTGAGCC CATCCGCACC CAGGACAGCA ACCCATCCAG GCCAGTGGAG 
GACCCATGGA GAGGCCAGCG GGGACTGGCT GAAGTGGCTG TGCATGTGCA GGAGCCCGTA 
GTCCTTGGGC TCCGGACCTT GCAGGTGTCC TGGACTGTAG ATGGCCCAGT CCAGCTGGTG 
CAAGGTTTCC GGGTGTCTTG GAGGGTAGCA GGTCCTGATG GGGGAAGCTG GACAGTACTG 
GACCTACAGT CCCCAAGCCA GCAAAGTACT GTGCTAAGAG GACTCCCCCC AGGGACCCAA 
ATTCAGATCA AAGTGCAAGC CCAAGGCCAG GAGGGGCTGG GGCCTGAAAG TCCCTTTGTG 
ACCAGGAGCA TTCCGGAGGA AGCCCCCAGT GGCCCCCCCC AGGGAGTGAC AGTGGCCTTG 
GGGGGTGAAG GCAACAGCAG TGTCACGGTG TCCTGGGAAC CTCCCCTCCC CTCCCAGCAA 
AATGGGGTGA TCAAGGAATA CCAGATCTGG TGCCTGGGCA ATGAGAGCCG CTTCCACCTC 
AACCGGTCTG CGGCAGGCTG GGCACGCTCC GCCGTGCTCC GGGGACTGCT GCCCGGCCTT 
CTCTACCGGA CCCGGGTCGC GGCGGCCACC AGCGCCGGCG TGGGCGTGGC CAGCGTCCCG 
GTGTCGGTGC AGCTGCCCTC CCCGCCGGAG TCGGAGCCCG GGCTCGAGGT GCGCCCGGGG 
CTGGCGGAGC GCCTGGCCAG GGTGCTGCGG GCGCCTGCCT TCCTGGCGGG CAGCGGGGCC 
GCCTGCGGGG CGCTGCTGCT CGCGCTCTGC GCCGTCCTCT ACCGGCGCCG GAGGCAGCGC 
AAGGAGCTCA GTCACTACAC GGCCTCCTTT GCCTACACAC CGGCAGTGTC CTTCCCACAC 
TCAGAGGGCC TCTCTGGAGC CAGTTCCAGG CCACCCATGG GCCTCGGCCC CGCCCCCTAC 
CCTTGGCTGG CCGACTCGTG GCCCCACCCA TCTCGAAGCC CCTCAGCCCC GGAGCCCAGG 
GGGAGCTGCT GCCCCAGCAA TCCTGACCCA GACGACAGAT ATTACAATGA AGCAGGGATC 
TCCCTGTACC TGGCTCAGAC GGCCCGAGGC ACTGCCGCCC CTACAGAGGG TCCTGTCTAC 
AGCACCATTG ACCCAGCTGG GGAGGAGCTG CAGACCTTCC ACGGGGGGTT CCCCCCAAAT 
CCCTCGGGGG ACCCAGGCGC CTGGAGCCAA TATGCTCCTC CAGAATGGAG CCAGGGGGAC 
AGTGGAGCCA GGGGAGGCAA AGTAAAGCTT CTGGGAAAAG CTGTGCAGAT GCCCTCTCTC 
AACTGGCCGG AAGCCCTGCC ACCACCTCCC CCTTCCTGTG AACTGAGCTG CCTAGAGGGG 
CCTGAGGAAG ATCTGGAAGG CAGCTCAGAG CCAGAAGAGT GGTGCCCACC AATGTCTGAG 
AGGAGCCATC TGACAGAGCC CAGCTCCACT GGAGGGTGCT TGGTCCCTCC ATCCCAAGGG 
GAAGCCCCCT CTCCCACATC TTCCTATGGA CAGCAGTCCA CAGCTACTCT TACCCCCTCA 
CCTCCTGACC CTCCCCAGCC CCCCACTGAC ATCCCCCATC TCCACCAGAT GCCCAGGAGG 
GTGCCCCTGG GGCCAAGTTC CCCTCTCAGT GTATCCCAGC CCACTCTGAG TAGCCATGAA 
GGGAGGCCTG CTGGCCTGGG TGCTGGCCCC ACAGCCTCCC ATCACCTCAA CCCCAGTCCT 
GTCCCTAGTA CAGCCAGCAG TGCCCCAGGG AGAACCCGGC AGGTGCCTGG GGAGATGACC 
CCTCCGCTTC AAGGGCCCCG GGCCCGAATC CGGAAGCCCA AGGCTGTTCC CTACCGGCGG 
GAGCACAGTC CTGGCGATTT GCCCCCACCA CCCTTGCCGC CACCAGAGGA AGAGACGAGC 
TGGGCCTTAG GGCTGAGAGC AGCAGGCAGC ATGTCCTCCC TGGAACGAGA GCGGGAGCGC 
AGTGGGGAGA GAATGGTGCA GGCTGGGCTC CTGGGGGCCC AGCGGGGTCC CCACCTGGAT 
GAAGAGGCCT GGCTCCCTTA CAGCCGACCG AGCTTCCTGT CCCGGGGCCA GGGGCCCAAC 
ACCTGTTCCA CAGCCGGCAG CAACTCTTCG AGAGGCTCTG GCAGCTCTCG AGGCTCCCGG 
GGACCTGGAC GGAGCCGGAG CCGGAGCCGG AGCCAGAGCC AGAGGCCAGG ACAGAAATGT 
GGAGAGGTGC GGGCTAGGGA ACCAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968804.1
CDS	95..4264
Translation	MLRYLLKTLLQMNLFADSLAGDISNSSDLLFGFNSSVAALNHSLLPPGDPSFNASRVEPEDAMPRIVEQPPDLLVSRGEPATLPCRAEGRPRPNIEWYKNGARVATAREDPRAHRLLLPSGALFFPRIVHGRRARPDEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVMECVPPRGHPEPSVSWKKDSARLREEEGRITIRGGKLMMSHTLKSDAGMYVCVASNMAGERESGAAELVVLERPSFLRRPVNQVVLADAPVDFPCEVQGDPPPRLRWRKEDGELPTGRYEIRSDHSLWIGRVTAADEGTYTCVAENSVGRAEASGSLSVHVPPQLVTQPQDQMAAPGESVAFQCETKGNPPPAIFWQKEGSQVLLFPSQPLQPKGRFSVSPRGQLNITDVQSGDAGYYVCQAVSVAGSVLAKALLEVKRASSLDGLPPIILQGPANQTLALGSPVWLPCRVTGNPQPSVRWKKDGQWLQGDDLQLNLMANGTLYIASVQEMDLGFYSCVAKSSMGEATWSSWLRRREDWGVSPEPPTEPSTPPGPPSQPVVTEITKNSITLTWKPNPQAGATVTSYVIEAFSQAAGNTWRTVADGVQLETHTVSGLQPNTIYLFLVRAVGAWGLSEPSPVSEPIRTQDSNPSRPVEDPWRGQRGLAEVAVHVQEPVVLGLRTLQVSWTVDGPVQLVQGFRVSWRVAGPDGGSWTVLDLQSPSQQSTVLRGLPPGTQIQIKVQAQGQEGLGPESPFVTRSIPEEAPSGPPQGVTVALGGEGNSSVTVSWEPPLPSQQNGVIKEYQIWCLGNESRFHLNRSAAGWARSAVLRGLLPGLLYRTRVAAATSAGVGVASVPVSVQLPSPPESEPGLEVRPGLAERLARVLRAPAFLAGSGAACGALLLALCAVLYRRRRQRKELSHYTASFAYTPAVSFPHSEGLSGASSRPPMGLGPAPYPWLADSWPHPSRSPSAPEPRGSCCPSNPDPDDRYYNEAGISLYLAQTARGTAAPTEGPVYSTIDPAGEELQTFHGGFPPNPSGDPGAWSQYAPPEWSQGDSGARGGKVKLLGKAVQMPSLNWPEALPPPPPSCELSCLEGPEEDLEGSSEPEEWCPPMSERSHLTEPSSTGGCLVPPSQGEAPSPTSSYGQQSTATLTPSPPDPPQPPTDIPHLHQMPRRVPLGPSSPLSVSQPTLSSHEGRPAGLGAGPTASHHLNPSPVPSTASSAPGRTRQVPGEMTPPLQGPRARIRKPKAVPYRREHSPGDLPPPPLPPPEEETSWALGLRAAGSMSSLERERERSGERMVQAGLLGAQRGPHLDEEAWLPYSRPSFLSRGQGPNTCSTAGSNSSRGSGSSRGSRGPGRSRSRSRSQSQRPGQKCGEVRAREPR

Target ORF information:

RefSeq Version	XM_014113329.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113329.2

ORF Insert Sequence:

ATGCTGCGCT ACCTGCTGAA GACACTGCTG CAGATGAACT TGTTCGCGGA CTCCCTGGCG 
GGGGACATCT CCAACTCCAG CGACCTGCTC TTCGGCTTCA ACTCCTCGGT GGCGGCGCTC 
AACCACAGCC TGCTGCCCCC CGGCGACCCC TCTTTCAACG CGTCAAGGGT CGAGCCGGAG 
GACGCGATGC CGCGCATCGT GGAGCAGCCG CCAGATCTGC TGGTGTCCCG GGGCGAGCCG 
GCCACGCTGC CCTGCCGCGC CGAGGGCCGG CCCCGGCCCA ACATCGAGTG GTACAAGAAC 
GGGGCGCGGG TGGCCACTGC GCGCGAGGAC CCGCGCGCGC ACCGCCTGCT GCTGCCCAGC 
GGCGCCCTCT TCTTCCCCCG CATCGTGCAC GGGCGCCGCG CGCGCCCCGA CGAGGGTGTC 
TACACTTGCG TGGCGCGCAA CTACCTGGGG GCAGCGGCCA GCAGAAATGC CTCGCTGGAA 
GTGGCGGTCC TCCGTGATGA TTTCCGGCAG TCTCCTGGAA ACGTGGTGGT GGCAGTGGGC 
GAGCCAGCGG TGATGGAATG CGTTCCCCCC CGTGGCCACC CGGAGCCTTC CGTGTCCTGG 
AAGAAGGACA GTGCAAGACT CAGAGAGGAG GAGGGAAGGA TCACGATTCG TGGAGGGAAG 
CTGATGATGT CACATACACT CAAGAGTGAT GCGGGGATGT ATGTGTGCGT GGCCTCCAAC 
ATGGCAGGAG AACGGGAGAG TGGGGCAGCT GAACTTGTGG TACTGGAGCG ACCCTCATTC 
CTGCGTAGAC CAGTCAACCA GGTGGTCCTG GCAGATGCCC CCGTGGATTT CCCATGTGAG 
GTGCAGGGAG ATCCCCCACC CCGTCTACGC TGGCGCAAGG AGGATGGGGA ACTGCCCACC 
GGCAGGTATG AGATCCGGAG TGACCACAGC CTTTGGATCG GCCGCGTGAC TGCCGCCGAC 
GAGGGGACCT ACACCTGTGT GGCTGAGAAC AGCGTGGGCC GCGCCGAAGC ATCTGGCTCC 
CTCAGTGTTC ACGTCCCACC CCAGCTGGTG ACCCAGCCGC AGGACCAGAT GGCAGCTCCT 
GGAGAGAGTG TGGCTTTCCA ATGCGAGACC AAAGGAAACC CCCCACCTGC CATCTTCTGG 
CAGAAGGAGG GAAGTCAGGT TCTACTTTTC CCCAGCCAGC CGCTTCAGCC CAAGGGGCGC 
TTCTCTGTGT CTCCCAGAGG CCAGCTCAAC ATTACGGATG TGCAGAGCGG GGATGCTGGC 
TACTACGTGT GTCAGGCCGT TAGTGTGGCC GGGAGCGTCC TGGCTAAGGC CCTGCTGGAG 
GTAAAAAGAG CCTCCTCCTT GGATGGGCTG CCTCCTATCA TCCTCCAGGG ACCAGCCAAT 
CAGACGCTGG CACTTGGCTC CCCTGTGTGG CTGCCATGCA GAGTGACCGG GAACCCTCAA 
CCGAGTGTCC GATGGAAGAA GGATGGGCAG TGGCTGCAGG GGGACGACCT CCAGCTCAAC 
CTAATGGCCA ATGGTACTCT GTACATCGCC AGCGTGCAGG AGATGGACCT GGGTTTCTAC 
AGCTGTGTGG CCAAGAGTTC CATGGGGGAG GCCACATGGA GCAGCTGGCT TAGGAGGCGG 
GAAGATTGGG GAGTATCACC AGAGCCCCCT ACAGAACCCA GTACCCCTCC AGGGCCTCCC 
TCTCAGCCAG TGGTCACTGA GATCACCAAG AACAGCATTA CCCTGACCTG GAAGCCCAAC 
CCGCAGGCGG GGGCCACAGT CACCTCTTAT GTGATAGAGG CCTTCAGCCA AGCAGCTGGC 
AACACGTGGC GGACAGTGGC AGATGGTGTG CAGCTGGAGA CACACACGGT CAGTGGTCTG 
CAGCCCAACA CCATCTATCT GTTTCTGGTG CGAGCTGTGG GAGCCTGGGG CCTCAGTGAG 
CCCAGCCCCG TCTCTGAGCC CATCCGCACC CAGGACAGCA ACCCATCCAG GCCAGTGGAG 
GACCCATGGA GAGGCCAGCG GGGACTGGCT GAAGTGGCTG TGCATGTGCA GGAGCCCGTA 
GTCCTTGGGC TCCGGACCTT GCAGGTGTCC TGGACTGTAG ATGGCCCAGT CCAGCTGGTG 
CAAGGTTTCC GGGTGTCTTG GAGGGTAGCA GGTCCTGATG GGGGAAGCTG GACAGTACTG 
GACCTACAGT CCCCAAGCCA GCAAAGTACT GTGCTAAGAG GACTCCCCCC AGGGACCCAA 
ATTCAGATCA AAGTGCAAGC CCAAGGCCAG GAGGGGCTGG GGCCTGAAAG TCCCTTTGTG 
ACCAGGAGCA TTCCGGAGGA AGCCCCCAGT GGCCCCCCCC AGGGAGTGAC AGTGGCCTTG 
GGGGGTGAAG GCAACAGCAG TGTCACGGTG TCCTGGGAAC CTCCCCTCCC CTCCCAGCAA 
AATGGGGTGA TCAAGGAATA CCAGATCTGG TGCCTGGGCA ATGAGAGCCG CTTCCACCTC 
AACCGGTCTG CGGCAGGCTG GGCACGCTCC GCCGTGCTCC GGGGACTGCT GCCCGGCCTT 
CTCTACCGGA CCCGGGTCGC GGCGGCCACC AGCGCCGGCG TGGGCGTGGC CAGCGTCCCG 
GTGTCGGTGC AGCTGCCCTC CCCGCCGGAG TCGGAGCCCG GGCTCGAGGT GCGCCCGGGG 
CTGGCGGAGC GCCTGGCCAG GGTGCTGCGG GCGCCTGCCT TCCTGGCGGG CAGCGGGGCC 
GCCTGCGGGG CGCTGCTGCT CGCGCTCTGC GCCGTCCTCT ACCGGCGCCG GAGGCAGCGC 
AAGGAGCTCA GTCACTACAC GGCCTCCTTT GCCTACACAC CGGCAGTGTC CTTCCCACAC 
TCAGAGGGCC TCTCTGGAGC CAGTTCCAGG CCACCCATGG GCCTCGGCCC CGCCCCCTAC 
CCTTGGCTGG CCGACTCGTG GCCCCACCCA TCTCGAAGCC CCTCAGCCCC GGAGCCCAGG 
GGGAGCTGCT GCCCCAGCAA TCCTGACCCA GACGACAGAT ATTACAATGA AGCAGGGATC 
TCCCTGTACC TGGCTCAGAC GGCCCGAGGC ACTGCCGCCC CTACAGAGGG TCCTGTCTAC 
AGCACCATTG ACCCAGCTGG GGAGGAGCTG CAGACCTTCC ACGGGGGGTT CCCCCCAAAT 
CCCTCGGGGG ACCCAGGCGC CTGGAGCCAA TATGCTCCTC CAGAATGGAG CCAGGGGGAC 
AGTGGAGCCA GGGGAGGCAA AGTAAAGCTT CTGGGAAAAG CTGTGCAGAT GCCCTCTCTC 
AACTGGCCGG AAGCCCTGCC ACCACCTCCC CCTTCCTGTG AACTGAGCTG CCTAGAGGGG 
CCTGAGGAAG ATCTGGAAGG CAGCTCAGAG CCAGAAGAGT GGTGCCCACC AATGTCTGAG 
AGGAGCCATC TGACAGAGCC CAGCTCCACT GGAGGGTGCT TGGTCCCTCC ATCCCAAGGG 
GAAGCCCCCT CTCCCACATC TTCCTATGGA CAGCAGTCCA CAGCTACTCT TACCCCCTCA 
CCTCCTGACC CTCCCCAGCC CCCCACTGAC ATCCCCCATC TCCACCAGAT GCCCAGGAGG 
GTGCCCCTGG GGCCAAGTTC CCCTCTCAGT GTATCCCAGC CCACTCTGAG TAGCCATGAA 
GGGAGGCCTG CTGGCCTGGG TGCTGGCCCC ACAGCCTCCC ATCACCTCAA CCCCAGTCCT 
GTCCCTAGTA CAGCCAGCAG TGCCCCAGGG AGAACCCGGC AGGTGCCTGG GGAGATGACC 
CCTCCGCTTC AAGGGCCCCG GGCCCGAATC CGGAAGCCCA AGGCTGTTCC CTACCGGCGG 
GAGCACAGTC CTGGCGATTT GCCCCCACCA CCCTTGCCGC CACCAGAGGA AGAGACGAGC 
TGGGCCTTAG GGCTGAGAGC AGCAGGCAGC ATGTCCTCCC TGGAACGAGA GCGGGAGCGC 
AGTGGGGAGA GAATGGTGCA GGCTGGGCTC CTGGGGGCCC AGCGGGGTCC CCACCTGGAT 
GAAGAGGCCT GGCTCCCTTA CAGCCGACCG AGCTTCCTGT CCCGGGGCCA GGGGCCCAAC 
ACCTGTTCCA CAGCCGGCAG CAACTCTTCG AGAGGCTCTG GCAGCTCTCG AGGCTCCCGG 
GGACCTGGAC GGAGCCGGAG CCGGAGCCGG AGCCAGAGCC AGAGGCCAGG ACAGAAATGT 
GGAGAGGTGC GGGCTAGGGA ACCAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO3 ( XM_014113330.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968805.1 Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X6, mRNA.

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CloneID	OCe36284
Clone ID Related Accession (Same CDS sequence)	XM_014113330.1
Accession Version	XM_014113330.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	roundabout homolog 3 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGCT ACCTGCTGAA GACACTGCTG CAGATGAACT TGTTCGCGGA CTCCCTGGCG 
GGGGACATCT CCAACTCCAG CGACCTGCTC TTCGGCTTCA ACTCCTCGGT GGCGGCGCTC 
AACCACAGCC TGCTGCCCCC CGGCGACCCC TCTTTCAACG CGTCAAGGGT CGAGCCGGAG 
GACGCGATGC CGCGCATCGT GGAGCAGCCG CCAGATCTGC TGGTGTCCCG GGGCGAGCCG 
GCCACGCTGC CCTGCCGCGC CGAGGGCCGG CCCCGGCCCA ACATCGAGTG GTACAAGAAC 
GGGGCGCGGG TGGCCACTGC GCGCGAGGAC CCGCGCGCGC ACCGCCTGCT GCTGCCCAGC 
GGCGCCCTCT TCTTCCCCCG CATCGTGCAC GGGCGCCGCG CGCGCCCCGA CGAGGGTGTC 
TACACTTGCG TGGCGCGCAA CTACCTGGGG GCAGCGGCCA GCAGAAATGC CTCGCTGGAA 
GTGGCGGTCC TCCGTGATGA TTTCCGGCAG TCTCCTGGAA ACGTGGTGGT GGCAGTGGGC 
GAGCCAGCGG TGATGGAATG CGTTCCCCCC CGTGGCCACC CGGAGCCTTC CGTGTCCTGG 
AAGAAGGACA GTGCAAGACT CAGAGAGGAG GAGGGAAGGA TCACGATTCG TGGAGGGAAG 
CTGATGATGT CACATACACT CAAGAGTGAT GCGGGGATGT ATGTGTGCGT GGCCTCCAAC 
ATGGCAGGAG AACGGGAGAG TGGGGCAGCT GAACTTGTGG TACTGGAGCG ACCCTCATTC 
CTGCGTAGAC CAGTCAACCA GGTGGTCCTG GCAGATGCCC CCGTGGATTT CCCATGTGAG 
GTGCAGGGAG ATCCCCCACC CCGTCTACGC TGGCGCAAGG AGGATGGGGA ACTGCCCACC 
GGCAGGTATG AGATCCGGAG TGACCACAGC CTTTGGATCG GCCGCGTGAC TGCCGCCGAC 
GAGGGGACCT ACACCTGTGT GGCTGAGAAC AGCGTGGGCC GCGCCGAAGC ATCTGGCTCC 
CTCAGTGTTC ACGTCCCACC CCAGCTGGTG ACCCAGCCGC AGGACCAGAT GGCAGCTCCT 
GGAGAGAGTG TGGCTTTCCA ATGCGAGACC AAAGGAAACC CCCCACCTGC CATCTTCTGG 
CAGAAGGAGG GAAGTCAGGT TCTACTTTTC CCCAGCCAGC CGCTTCAGCC CAAGGGGCGC 
TTCTCTGTGT CTCCCAGAGG CCAGCTCAAC ATTACGGATG TGCAGAGCGG GGATGCTGGC 
TACTACGTGT GTCAGGCCGT TAGTGTGGCC GGGAGCGTCC TGGCTAAGGC CCTGCTGGAG 
GTAAAAAGAG CCTCCTCCTT GGATGGGCTG CCTCCTATCA TCCTCCAGGG ACCAGCCAAT 
CAGACGCTGG CACTTGGCTC CCCTGTGTGG CTGCCATGCA GAGTGACCGG GAACCCTCAA 
CCGAGTGTCC GATGGAAGAA GGATGGGCAG TGGCTGCAGG GGGACGACCT CCAGCTCAAC 
CTAATGGCCA ATGGTACTCT GTACATCGCC AGCGTGCAGG AGATGGACCT GGGTTTCTAC 
AGCTGTGTGG CCAAGAGTTC CATGGGGGAG GCCACATGGA GCAGCTGGCT TAGGAGGCGG 
GATTATCTCC TCGCTGCAGA AGATTGGGGA GTATCACCAG AGCCCCCTAC AGAACCCAGT 
ACCCCTCCAG GGCCTCCCTC TCAGCCAGTG GTCACTGAGA TCACCAAGAA CAGCATTACC 
CTGACCTGGA AGCCCAACCC GCAGGCGGGG GCCACAGTCA CCTCTTATGT GATAGAGGCC 
TTCAGCCAAG CAGCTGGCAA CACGTGGCGG ACAGTGGCAG ATGGTGTGCA GCTGGAGACA 
CACACGGTCA GTGGTCTGCA GCCCAACACC ATCTATCTGT TTCTGGTGCG AGCTGTGGGA 
GCCTGGGGCC TCAGTGAGCC CAGCCCCGTC TCTGAGCCCA TCCGCACCCA GGACAGCAAC 
CCATCCAGGC CAGTGGAGGA CCCATGGAGA GGCCAGCGGG GACTGGCTGA AGTGGCTGTG 
CATGTGCAGG AGCCCGTAGT CCTTGGGCTC CGGACCTTGC AGGTGTCCTG GACTGTAGAT 
GGCCCAGTCC AGCTGGTGCA AGGTTTCCGG GTGTCTTGGA GGGTAGCAGG TCCTGATGGG 
GGAAGCTGGA CAGTACTGGA CCTACAGTCC CCAAGCCAGC AAAGTACTGT GCTAAGAGGA 
CTCCCCCCAG GGACCCAAAT TCAGATCAAA GTGCAAGCCC AAGGCCAGGA GGGGCTGGGG 
CCTGAAAGTC CCTTTGTGAC CAGGAGCATT CCGGAGGAAG CCCCCAGTGG CCCCCCCCAG 
GGAGTGACAG TGGCCTTGGG GGGTGAAGGC AACAGCAGTG TCACGGTGTC CTGGGAACCT 
CCCCTCCCCT CCCAGCAAAA TGGGGTGATC AAGGAATACC AGATCTGGTG CCTGGGCAAT 
GAGAGCCGCT TCCACCTCAA CCGGTCTGCG GCAGGCTGGG CACGCTCCGC CGTGCTCCGG 
GGACTGCTGC CCGGCCTTCT CTACCGGACC CGGGTCGCGG CGGCCACCAG CGCCGGCGTG 
GGCGTGGCCA GCGTCCCGGT GTCGGTGCAG CTGCCCTCCC CGCCGGAGTC GGAGCCCGGG 
CTCGAGGTGC GCCCGGGGCT GGCGGAGCGC CTGGCCAGGG TGCTGCGGGC GCCTGCCTTC 
CTGGCGGGCA GCGGGGCCGC CTGCGGGGCG CTGCTGCTCG CGCTCTGCGC CGTCCTCTAC 
CGGCGCCGGA GGCAGCGCAA GGAGCTCAGT CACTACACGG CCTCCTTTGC CTACACACCG 
GCAGTGTCCT TCCCACACTC AGAGGGCCTC TCTGGAGCCA GTTCCAGGCC ACCCATGGGC 
CTCGGCCCCG CCCCCTACCC TTGGCTGGCC GACTCGTGGC CCCACCCATC TCGAAGCCCC 
TCAGCCCCGG AGCCCAGGGG GAGCTGCTGC CCCAGCAATC CTGACCCAGA CGACAGATAT 
TACAATGAAG CAGGGATCTC CCTGTACCTG GCTCAGACGG CCCGAGGCAC TGCCGCCCCT 
ACAGAGGGTC CTGTCTACAG CACCATTGAC CCAGCTGGGG AGGAGCTGCA GACCTTCCAC 
GGGGGGTTCC CCCCAAATCC CTCGGGGGAC CCAGGCGCCT GGAGCCAATA TGCTCCTCCA 
GAATGGAGCC AGGGGGACAG TGGAGCCAGG GGAGGCAAAG TAAAGCTTCT GGGAAAAGCT 
GTGCAGATGC CCTCTCTCAA CTGGCCGGAA GCCCTGCCAC CACCTCCCCC TTCCTGTGAA 
CTGAGCTGCC TAGAGGGGCC TGAGGAAGAT CTGGAAGGCA GCTCAGAGCC AGAAGAGTGG 
TGCCCACCAA TGTCTGAGAG GAGCCATCTG ACAGAGCCCA GCTCCACTGG AGGGTGCTTG 
GTCCCTCCAT CCCAAGGGGA AGCCCCCTCT CCCACATCTT CCTATGGACA GCAGTCCACA 
GCTACTCTTA CCCCCTCACC TCCTGACCCT CCCCAGCCCC CCACTGACAT CCCCCATCTC 
CACCAGATGC CCAGTACAGC CAGCAGTGCC CCAGGGAGAA CCCGGCAGGT GCCTGGGGAG 
ATGACCCCTC CGCTTCAAGG GCCCCGGGCC CGAATCCGGA AGCCCAAGGC TGTTCCCTAC 
CGGCGGGAGC ACAGTCCTGG CGGTCTCTCC CCAGATTTGC CCCCACCACC CTTGCCGCCA 
CCAGAGGAAG AGACGAGCTG GGCCTTAGGG CTGAGAGCAG CAGGCAGCAT GTCCTCCCTG 
GAACGAGAGC GGGAGCGCAG TGGGGAGAGA ATGGTGCAGG CTGGGCTCCT GGGGGCCCAG 
CGGGGTCCCC ACCTGGATGA AGAGGCCTGG CTCCCTTACA GCCGACCGAG CTTCCTGTCC 
CGGGGCCAGG GGCCCAACAC CTGTTCCACA GCCGGCAGCA ACTCTTCGAG AGGCTCTGGC 
AGCTCTCGAG GCTCCCGGGG ACCTGGACGG AGCCGGAGCC GGAGCCGGAG CCAGAGCCAG 
AGGCCAGGAC AGAAATGTGG AGAGGTGCGG GCTAGGGAAC CAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968805.1
CDS	199..4266
Translation	MLRYLLKTLLQMNLFADSLAGDISNSSDLLFGFNSSVAALNHSLLPPGDPSFNASRVEPEDAMPRIVEQPPDLLVSRGEPATLPCRAEGRPRPNIEWYKNGARVATAREDPRAHRLLLPSGALFFPRIVHGRRARPDEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVMECVPPRGHPEPSVSWKKDSARLREEEGRITIRGGKLMMSHTLKSDAGMYVCVASNMAGERESGAAELVVLERPSFLRRPVNQVVLADAPVDFPCEVQGDPPPRLRWRKEDGELPTGRYEIRSDHSLWIGRVTAADEGTYTCVAENSVGRAEASGSLSVHVPPQLVTQPQDQMAAPGESVAFQCETKGNPPPAIFWQKEGSQVLLFPSQPLQPKGRFSVSPRGQLNITDVQSGDAGYYVCQAVSVAGSVLAKALLEVKRASSLDGLPPIILQGPANQTLALGSPVWLPCRVTGNPQPSVRWKKDGQWLQGDDLQLNLMANGTLYIASVQEMDLGFYSCVAKSSMGEATWSSWLRRRDYLLAAEDWGVSPEPPTEPSTPPGPPSQPVVTEITKNSITLTWKPNPQAGATVTSYVIEAFSQAAGNTWRTVADGVQLETHTVSGLQPNTIYLFLVRAVGAWGLSEPSPVSEPIRTQDSNPSRPVEDPWRGQRGLAEVAVHVQEPVVLGLRTLQVSWTVDGPVQLVQGFRVSWRVAGPDGGSWTVLDLQSPSQQSTVLRGLPPGTQIQIKVQAQGQEGLGPESPFVTRSIPEEAPSGPPQGVTVALGGEGNSSVTVSWEPPLPSQQNGVIKEYQIWCLGNESRFHLNRSAAGWARSAVLRGLLPGLLYRTRVAAATSAGVGVASVPVSVQLPSPPESEPGLEVRPGLAERLARVLRAPAFLAGSGAACGALLLALCAVLYRRRRQRKELSHYTASFAYTPAVSFPHSEGLSGASSRPPMGLGPAPYPWLADSWPHPSRSPSAPEPRGSCCPSNPDPDDRYYNEAGISLYLAQTARGTAAPTEGPVYSTIDPAGEELQTFHGGFPPNPSGDPGAWSQYAPPEWSQGDSGARGGKVKLLGKAVQMPSLNWPEALPPPPPSCELSCLEGPEEDLEGSSEPEEWCPPMSERSHLTEPSSTGGCLVPPSQGEAPSPTSSYGQQSTATLTPSPPDPPQPPTDIPHLHQMPSTASSAPGRTRQVPGEMTPPLQGPRARIRKPKAVPYRREHSPGGLSPDLPPPPLPPPEEETSWALGLRAAGSMSSLERERERSGERMVQAGLLGAQRGPHLDEEAWLPYSRPSFLSRGQGPNTCSTAGSNSSRGSGSSRGSRGPGRSRSRSRSQSQRPGQKCGEVRAREPR

Target ORF information:

RefSeq Version	XM_014113330.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113330.1

ORF Insert Sequence:

ATGCTGCGCT ACCTGCTGAA GACACTGCTG CAGATGAACT TGTTCGCGGA CTCCCTGGCG 
GGGGACATCT CCAACTCCAG CGACCTGCTC TTCGGCTTCA ACTCCTCGGT GGCGGCGCTC 
AACCACAGCC TGCTGCCCCC CGGCGACCCC TCTTTCAACG CGTCAAGGGT CGAGCCGGAG 
GACGCGATGC CGCGCATCGT GGAGCAGCCG CCAGATCTGC TGGTGTCCCG GGGCGAGCCG 
GCCACGCTGC CCTGCCGCGC CGAGGGCCGG CCCCGGCCCA ACATCGAGTG GTACAAGAAC 
GGGGCGCGGG TGGCCACTGC GCGCGAGGAC CCGCGCGCGC ACCGCCTGCT GCTGCCCAGC 
GGCGCCCTCT TCTTCCCCCG CATCGTGCAC GGGCGCCGCG CGCGCCCCGA CGAGGGTGTC 
TACACTTGCG TGGCGCGCAA CTACCTGGGG GCAGCGGCCA GCAGAAATGC CTCGCTGGAA 
GTGGCGGTCC TCCGTGATGA TTTCCGGCAG TCTCCTGGAA ACGTGGTGGT GGCAGTGGGC 
GAGCCAGCGG TGATGGAATG CGTTCCCCCC CGTGGCCACC CGGAGCCTTC CGTGTCCTGG 
AAGAAGGACA GTGCAAGACT CAGAGAGGAG GAGGGAAGGA TCACGATTCG TGGAGGGAAG 
CTGATGATGT CACATACACT CAAGAGTGAT GCGGGGATGT ATGTGTGCGT GGCCTCCAAC 
ATGGCAGGAG AACGGGAGAG TGGGGCAGCT GAACTTGTGG TACTGGAGCG ACCCTCATTC 
CTGCGTAGAC CAGTCAACCA GGTGGTCCTG GCAGATGCCC CCGTGGATTT CCCATGTGAG 
GTGCAGGGAG ATCCCCCACC CCGTCTACGC TGGCGCAAGG AGGATGGGGA ACTGCCCACC 
GGCAGGTATG AGATCCGGAG TGACCACAGC CTTTGGATCG GCCGCGTGAC TGCCGCCGAC 
GAGGGGACCT ACACCTGTGT GGCTGAGAAC AGCGTGGGCC GCGCCGAAGC ATCTGGCTCC 
CTCAGTGTTC ACGTCCCACC CCAGCTGGTG ACCCAGCCGC AGGACCAGAT GGCAGCTCCT 
GGAGAGAGTG TGGCTTTCCA ATGCGAGACC AAAGGAAACC CCCCACCTGC CATCTTCTGG 
CAGAAGGAGG GAAGTCAGGT TCTACTTTTC CCCAGCCAGC CGCTTCAGCC CAAGGGGCGC 
TTCTCTGTGT CTCCCAGAGG CCAGCTCAAC ATTACGGATG TGCAGAGCGG GGATGCTGGC 
TACTACGTGT GTCAGGCCGT TAGTGTGGCC GGGAGCGTCC TGGCTAAGGC CCTGCTGGAG 
GTAAAAAGAG CCTCCTCCTT GGATGGGCTG CCTCCTATCA TCCTCCAGGG ACCAGCCAAT 
CAGACGCTGG CACTTGGCTC CCCTGTGTGG CTGCCATGCA GAGTGACCGG GAACCCTCAA 
CCGAGTGTCC GATGGAAGAA GGATGGGCAG TGGCTGCAGG GGGACGACCT CCAGCTCAAC 
CTAATGGCCA ATGGTACTCT GTACATCGCC AGCGTGCAGG AGATGGACCT GGGTTTCTAC 
AGCTGTGTGG CCAAGAGTTC CATGGGGGAG GCCACATGGA GCAGCTGGCT TAGGAGGCGG 
GATTATCTCC TCGCTGCAGA AGATTGGGGA GTATCACCAG AGCCCCCTAC AGAACCCAGT 
ACCCCTCCAG GGCCTCCCTC TCAGCCAGTG GTCACTGAGA TCACCAAGAA CAGCATTACC 
CTGACCTGGA AGCCCAACCC GCAGGCGGGG GCCACAGTCA CCTCTTATGT GATAGAGGCC 
TTCAGCCAAG CAGCTGGCAA CACGTGGCGG ACAGTGGCAG ATGGTGTGCA GCTGGAGACA 
CACACGGTCA GTGGTCTGCA GCCCAACACC ATCTATCTGT TTCTGGTGCG AGCTGTGGGA 
GCCTGGGGCC TCAGTGAGCC CAGCCCCGTC TCTGAGCCCA TCCGCACCCA GGACAGCAAC 
CCATCCAGGC CAGTGGAGGA CCCATGGAGA GGCCAGCGGG GACTGGCTGA AGTGGCTGTG 
CATGTGCAGG AGCCCGTAGT CCTTGGGCTC CGGACCTTGC AGGTGTCCTG GACTGTAGAT 
GGCCCAGTCC AGCTGGTGCA AGGTTTCCGG GTGTCTTGGA GGGTAGCAGG TCCTGATGGG 
GGAAGCTGGA CAGTACTGGA CCTACAGTCC CCAAGCCAGC AAAGTACTGT GCTAAGAGGA 
CTCCCCCCAG GGACCCAAAT TCAGATCAAA GTGCAAGCCC AAGGCCAGGA GGGGCTGGGG 
CCTGAAAGTC CCTTTGTGAC CAGGAGCATT CCGGAGGAAG CCCCCAGTGG CCCCCCCCAG 
GGAGTGACAG TGGCCTTGGG GGGTGAAGGC AACAGCAGTG TCACGGTGTC CTGGGAACCT 
CCCCTCCCCT CCCAGCAAAA TGGGGTGATC AAGGAATACC AGATCTGGTG CCTGGGCAAT 
GAGAGCCGCT TCCACCTCAA CCGGTCTGCG GCAGGCTGGG CACGCTCCGC CGTGCTCCGG 
GGACTGCTGC CCGGCCTTCT CTACCGGACC CGGGTCGCGG CGGCCACCAG CGCCGGCGTG 
GGCGTGGCCA GCGTCCCGGT GTCGGTGCAG CTGCCCTCCC CGCCGGAGTC GGAGCCCGGG 
CTCGAGGTGC GCCCGGGGCT GGCGGAGCGC CTGGCCAGGG TGCTGCGGGC GCCTGCCTTC 
CTGGCGGGCA GCGGGGCCGC CTGCGGGGCG CTGCTGCTCG CGCTCTGCGC CGTCCTCTAC 
CGGCGCCGGA GGCAGCGCAA GGAGCTCAGT CACTACACGG CCTCCTTTGC CTACACACCG 
GCAGTGTCCT TCCCACACTC AGAGGGCCTC TCTGGAGCCA GTTCCAGGCC ACCCATGGGC 
CTCGGCCCCG CCCCCTACCC TTGGCTGGCC GACTCGTGGC CCCACCCATC TCGAAGCCCC 
TCAGCCCCGG AGCCCAGGGG GAGCTGCTGC CCCAGCAATC CTGACCCAGA CGACAGATAT 
TACAATGAAG CAGGGATCTC CCTGTACCTG GCTCAGACGG CCCGAGGCAC TGCCGCCCCT 
ACAGAGGGTC CTGTCTACAG CACCATTGAC CCAGCTGGGG AGGAGCTGCA GACCTTCCAC 
GGGGGGTTCC CCCCAAATCC CTCGGGGGAC CCAGGCGCCT GGAGCCAATA TGCTCCTCCA 
GAATGGAGCC AGGGGGACAG TGGAGCCAGG GGAGGCAAAG TAAAGCTTCT GGGAAAAGCT 
GTGCAGATGC CCTCTCTCAA CTGGCCGGAA GCCCTGCCAC CACCTCCCCC TTCCTGTGAA 
CTGAGCTGCC TAGAGGGGCC TGAGGAAGAT CTGGAAGGCA GCTCAGAGCC AGAAGAGTGG 
TGCCCACCAA TGTCTGAGAG GAGCCATCTG ACAGAGCCCA GCTCCACTGG AGGGTGCTTG 
GTCCCTCCAT CCCAAGGGGA AGCCCCCTCT CCCACATCTT CCTATGGACA GCAGTCCACA 
GCTACTCTTA CCCCCTCACC TCCTGACCCT CCCCAGCCCC CCACTGACAT CCCCCATCTC 
CACCAGATGC CCAGTACAGC CAGCAGTGCC CCAGGGAGAA CCCGGCAGGT GCCTGGGGAG 
ATGACCCCTC CGCTTCAAGG GCCCCGGGCC CGAATCCGGA AGCCCAAGGC TGTTCCCTAC 
CGGCGGGAGC ACAGTCCTGG CGGTCTCTCC CCAGATTTGC CCCCACCACC CTTGCCGCCA 
CCAGAGGAAG AGACGAGCTG GGCCTTAGGG CTGAGAGCAG CAGGCAGCAT GTCCTCCCTG 
GAACGAGAGC GGGAGCGCAG TGGGGAGAGA ATGGTGCAGG CTGGGCTCCT GGGGGCCCAG 
CGGGGTCCCC ACCTGGATGA AGAGGCCTGG CTCCCTTACA GCCGACCGAG CTTCCTGTCC 
CGGGGCCAGG GGCCCAACAC CTGTTCCACA GCCGGCAGCA ACTCTTCGAG AGGCTCTGGC 
AGCTCTCGAG GCTCCCGGGG ACCTGGACGG AGCCGGAGCC GGAGCCGGAG CCAGAGCCAG 
AGGCCAGGAC AGAAATGTGG AGAGGTGCGG GCTAGGGAAC CAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO3 ( XM_014113331.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968806.1 Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X7, mRNA.

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CloneID	OCe36285
Clone ID Related Accession (Same CDS sequence)	XM_014113331.1
Accession Version	XM_014113331.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	roundabout homolog 3 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGCT ACCTGCTGAA GACACTGCTG CAGATGAACT TGTTCGCGGA CTCCCTGGCG 
GGGGACATCT CCAACTCCAG CGACCTGCTC TTCGGCTTCA ACTCCTCGGT GGCGGCGCTC 
AACCACAGCC TGCTGCCCCC CGGCGACCCC TCTTTCAACG CGTCAAGGGT CGAGCCGGAG 
GACGCGATGC CGCGCATCGT GGAGCAGCCG CCAGATCTGC TGGTGTCCCG GGGCGAGCCG 
GCCACGCTGC CCTGCCGCGC CGAGGGCCGG CCCCGGCCCA ACATCGAGTG GTACAAGAAC 
GGGGCGCGGG TGGCCACTGC GCGCGAGGAC CCGCGCGCGC ACCGCCTGCT GCTGCCCAGC 
GGCGCCCTCT TCTTCCCCCG CATCGTGCAC GGGCGCCGCG CGCGCCCCGA CGAGGGTGTC 
TACACTTGCG TGGCGCGCAA CTACCTGGGG GCAGCGGCCA GCAGAAATGC CTCGCTGGAA 
GTGGCGGTCC TCCGTGATGA TTTCCGGCAG TCTCCTGGAA ACGTGGTGGT GGCAGTGGGC 
GAGCCAGCGG TGATGGAATG CGTTCCCCCC CGTGGCCACC CGGAGCCTTC CGTGTCCTGG 
AAGAAGGACA GTGCAAGACT CAGAGAGGAG GAGGGAAGGA TCACGATTCG TGGAGGGAAG 
CTGATGATGT CACATACACT CAAGAGTGAT GCGGGGATGT ATGTGTGCGT GGCCTCCAAC 
ATGGCAGGAG AACGGGAGAG TGGGGCAGCT GAACTTGTGG TACTGGAGCG ACCCTCATTC 
CTGCGTAGAC CAGTCAACCA GGTGGTCCTG GCAGATGCCC CCGTGGATTT CCCATGTGAG 
GTGCAGGGAG ATCCCCCACC CCGTCTACGC TGGCGCAAGG AGGATGGGGA ACTGCCCACC 
GGCAGGTATG AGATCCGGAG TGACCACAGC CTTTGGATCG GCCGCGTGAC TGCCGCCGAC 
GAGGGGACCT ACACCTGTGT GGCTGAGAAC AGCGTGGGCC GCGCCGAAGC ATCTGGCTCC 
CTCAGTGTTC ACGTCCCACC CCAGCTGGTG ACCCAGCCGC AGGACCAGAT GGCAGCTCCT 
GGAGAGAGTG TGGCTTTCCA ATGCGAGACC AAAGGAAACC CCCCACCTGC CATCTTCTGG 
CAGAAGGAGG GAAGTCAGGT TCTACTTTTC CCCAGCCAGC CGCTTCAGCC CAAGGGGCGC 
TTCTCTGTGT CTCCCAGAGG CCAGCTCAAC ATTACGGATG TGCAGAGCGG GGATGCTGGC 
TACTACGTGT GTCAGGCCGT TAGTGTGGCC GGGAGCGTCC TGGCTAAGGC CCTGCTGGAG 
GTAAAAAGAG CCTCCTCCTT GGATGGGCTG CCTCCTATCA TCCTCCAGGG ACCAGCCAAT 
CAGACGCTGG CACTTGGCTC CCCTGTGTGG CTGCCATGCA GAGTGACCGG GAACCCTCAA 
CCGAGTGTCC GATGGAAGAA GGATGGGCAG TGGCTGCAGG GGGACGACCT CCAGCTCAAC 
CTAATGGCCA ATGGTACTCT GTACATCGCC AGCGTGCAGG AGATGGACCT GGGTTTCTAC 
AGCTGTGTGG CCAAGAGTTC CATGGGGGAG GCCACATGGA GCAGCTGGCT TAGGAGGCGG 
GATTATCTCC TCGCTGCAGA AGATTGGGGA GTATCACCAG AGCCCCCTAC AGAACCCAGT 
ACCCCTCCAG GGCCTCCCTC TCAGCCAGTG GTCACTGAGA TCACCAAGAA CAGCATTACC 
CTGACCTGGA AGCCCAACCC GCAGGCGGGG GCCACAGTCA CCTCTTATGT GATAGAGGCC 
TTCAGCCAAG CAGCTGGCAA CACGTGGCGG ACAGTGGCAG ATGGTGTGCA GCTGGAGACA 
CACACGGTCA GTGGTCTGCA GCCCAACACC ATCTATCTGT TTCTGGTGCG AGCTGTGGGA 
GCCTGGGGCC TCAGTGAGCC CAGCCCCGTC TCTGAGCCCA TCCGCACCCA GGACAGCAAC 
CCATCCAGGC CAGTGGAGGA CCCATGGAGA GGCCAGCGGG GACTGGCTGA AGTGGCTGTG 
CATGTGCAGG AGCCCGTAGT CCTTGGGCTC CGGACCTTGC AGGTGTCCTG GACTGTAGAT 
GGCCCAGTCC AGCTGGTGCA AGGTTTCCGG GTGTCTTGGA GGGTAGCAGG TCCTGATGGG 
GGAAGCTGGA CAGTACTGGA CCTACAGTCC CCAAGCCAGC AAAGTACTGT GCTAAGAGGA 
CTCCCCCCAG GGACCCAAAT TCAGATCAAA GTGCAAGCCC AAGGCCAGGA GGGGCTGGGG 
CCTGAAAGTC CCTTTGTGAC CAGGAGCATT CCGGAGGAAG CCCCCAGTGG CCCCCCCCAG 
GGAGTGACAG TGGCCTTGGG GGGTGAAGGC AACAGCAGTG TCACGGTGTC CTGGGAACCT 
CCCCTCCCCT CCCAGCAAAA TGGGGTGATC AAGGAATACC AGATCTGGTG CCTGGGCAAT 
GAGAGCCGCT TCCACCTCAA CCGGTCTGCG GCAGGCTGGG CACGCTCCGC CGTGCTCCGG 
GGACTGCTGC CCGGCCTTCT CTACCGGACC CGGGTCGCGG CGGCCACCAG CGCCGGCGTG 
GGCGTGGCCA GCGTCCCGGT GTCGGTGCAG CTGCCCTCCC CGCCGGAGTC GGAGCCCGGG 
CTCGAGGTGC GCCCGGGGCT GGCGGAGCGC CTGGCCAGGG TGCTGCGGGC GCCTGCCTTC 
CTGGCGGGCA GCGGGGCCGC CTGCGGGGCG CTGCTGCTCG CGCTCTGCGC CGTCCTCTAC 
CGGCGCCGGA GGCAGCGCAA GGAGCTCAGT CACTACACGG CCTCCTTTGC CTACACACCG 
GCAGTGTCCT TCCCACACTC AGAGGGCCTC TCTGGAGCCA GTTCCAGGGG CTCACCTGGC 
CCCCGCCACC CTTCTCTCCA CCTCCTGGGG CAAACGGAAG GGAGCCGAGG ATTGCCAGTC 
TTCCTGAGGT TGGTCAGTCC TTGGGGAGGA TATGAGTTGG GGGAGCTTCT CTCATGCCTA 
ACCTTCACCC CGGCTTGGGA AGTAAGGTTT GGGGAGGGGG GAAGAAGCTG GTCCGGTGGG 
ATCTCCTTTG GCCCTCCATC CTCCTGTTGT CCGCTATTTC TCTGTCTCTG CCCTCTGCCC 
CCTGCCTCCC CCCCTGGCCT CCCTCTTTCT CCTCCTCAGA AGCAGGGATC TCCCTGTACC 
TGGCTCAGAC GGCCCGAGGC ACTGCCGCCC CTACAGAGGG TCCTGTCTAC AGCACCATTG 
ACCCAGCTGG GGAGGAGCTG CAGACCTTCC ACGGGGGGTT CCCCCCAAAT CCCTCGGGGG 
ACCCAGGCGC CTGGAGCCAA TATGCTCCTC CAGAATGGAG CCAGGGGGAC AGTGGAGCCA 
GGGGAGGCAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968806.1
CDS	240..3614
Translation	MLRYLLKTLLQMNLFADSLAGDISNSSDLLFGFNSSVAALNHSLLPPGDPSFNASRVEPEDAMPRIVEQPPDLLVSRGEPATLPCRAEGRPRPNIEWYKNGARVATAREDPRAHRLLLPSGALFFPRIVHGRRARPDEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVMECVPPRGHPEPSVSWKKDSARLREEEGRITIRGGKLMMSHTLKSDAGMYVCVASNMAGERESGAAELVVLERPSFLRRPVNQVVLADAPVDFPCEVQGDPPPRLRWRKEDGELPTGRYEIRSDHSLWIGRVTAADEGTYTCVAENSVGRAEASGSLSVHVPPQLVTQPQDQMAAPGESVAFQCETKGNPPPAIFWQKEGSQVLLFPSQPLQPKGRFSVSPRGQLNITDVQSGDAGYYVCQAVSVAGSVLAKALLEVKRASSLDGLPPIILQGPANQTLALGSPVWLPCRVTGNPQPSVRWKKDGQWLQGDDLQLNLMANGTLYIASVQEMDLGFYSCVAKSSMGEATWSSWLRRRDYLLAAEDWGVSPEPPTEPSTPPGPPSQPVVTEITKNSITLTWKPNPQAGATVTSYVIEAFSQAAGNTWRTVADGVQLETHTVSGLQPNTIYLFLVRAVGAWGLSEPSPVSEPIRTQDSNPSRPVEDPWRGQRGLAEVAVHVQEPVVLGLRTLQVSWTVDGPVQLVQGFRVSWRVAGPDGGSWTVLDLQSPSQQSTVLRGLPPGTQIQIKVQAQGQEGLGPESPFVTRSIPEEAPSGPPQGVTVALGGEGNSSVTVSWEPPLPSQQNGVIKEYQIWCLGNESRFHLNRSAAGWARSAVLRGLLPGLLYRTRVAAATSAGVGVASVPVSVQLPSPPESEPGLEVRPGLAERLARVLRAPAFLAGSGAACGALLLALCAVLYRRRRQRKELSHYTASFAYTPAVSFPHSEGLSGASSRGSPGPRHPSLHLLGQTEGSRGLPVFLRLVSPWGGYELGELLSCLTFTPAWEVRFGEGGRSWSGGISFGPPSSCCPLFLCLCPLPPASPPGLPLSPPQKQGSPCTWLRRPEALPPLQRVLSTAPLTQLGRSCRPSTGGSPQIPRGTQAPGANMLLQNGARGTVEPGEAK

Target ORF information:

RefSeq Version	XM_014113331.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris roundabout guidance receptor 3 (ROBO3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113331.1

ORF Insert Sequence:

ATGCTGCGCT ACCTGCTGAA GACACTGCTG CAGATGAACT TGTTCGCGGA CTCCCTGGCG 
GGGGACATCT CCAACTCCAG CGACCTGCTC TTCGGCTTCA ACTCCTCGGT GGCGGCGCTC 
AACCACAGCC TGCTGCCCCC CGGCGACCCC TCTTTCAACG CGTCAAGGGT CGAGCCGGAG 
GACGCGATGC CGCGCATCGT GGAGCAGCCG CCAGATCTGC TGGTGTCCCG GGGCGAGCCG 
GCCACGCTGC CCTGCCGCGC CGAGGGCCGG CCCCGGCCCA ACATCGAGTG GTACAAGAAC 
GGGGCGCGGG TGGCCACTGC GCGCGAGGAC CCGCGCGCGC ACCGCCTGCT GCTGCCCAGC 
GGCGCCCTCT TCTTCCCCCG CATCGTGCAC GGGCGCCGCG CGCGCCCCGA CGAGGGTGTC 
TACACTTGCG TGGCGCGCAA CTACCTGGGG GCAGCGGCCA GCAGAAATGC CTCGCTGGAA 
GTGGCGGTCC TCCGTGATGA TTTCCGGCAG TCTCCTGGAA ACGTGGTGGT GGCAGTGGGC 
GAGCCAGCGG TGATGGAATG CGTTCCCCCC CGTGGCCACC CGGAGCCTTC CGTGTCCTGG 
AAGAAGGACA GTGCAAGACT CAGAGAGGAG GAGGGAAGGA TCACGATTCG TGGAGGGAAG 
CTGATGATGT CACATACACT CAAGAGTGAT GCGGGGATGT ATGTGTGCGT GGCCTCCAAC 
ATGGCAGGAG AACGGGAGAG TGGGGCAGCT GAACTTGTGG TACTGGAGCG ACCCTCATTC 
CTGCGTAGAC CAGTCAACCA GGTGGTCCTG GCAGATGCCC CCGTGGATTT CCCATGTGAG 
GTGCAGGGAG ATCCCCCACC CCGTCTACGC TGGCGCAAGG AGGATGGGGA ACTGCCCACC 
GGCAGGTATG AGATCCGGAG TGACCACAGC CTTTGGATCG GCCGCGTGAC TGCCGCCGAC 
GAGGGGACCT ACACCTGTGT GGCTGAGAAC AGCGTGGGCC GCGCCGAAGC ATCTGGCTCC 
CTCAGTGTTC ACGTCCCACC CCAGCTGGTG ACCCAGCCGC AGGACCAGAT GGCAGCTCCT 
GGAGAGAGTG TGGCTTTCCA ATGCGAGACC AAAGGAAACC CCCCACCTGC CATCTTCTGG 
CAGAAGGAGG GAAGTCAGGT TCTACTTTTC CCCAGCCAGC CGCTTCAGCC CAAGGGGCGC 
TTCTCTGTGT CTCCCAGAGG CCAGCTCAAC ATTACGGATG TGCAGAGCGG GGATGCTGGC 
TACTACGTGT GTCAGGCCGT TAGTGTGGCC GGGAGCGTCC TGGCTAAGGC CCTGCTGGAG 
GTAAAAAGAG CCTCCTCCTT GGATGGGCTG CCTCCTATCA TCCTCCAGGG ACCAGCCAAT 
CAGACGCTGG CACTTGGCTC CCCTGTGTGG CTGCCATGCA GAGTGACCGG GAACCCTCAA 
CCGAGTGTCC GATGGAAGAA GGATGGGCAG TGGCTGCAGG GGGACGACCT CCAGCTCAAC 
CTAATGGCCA ATGGTACTCT GTACATCGCC AGCGTGCAGG AGATGGACCT GGGTTTCTAC 
AGCTGTGTGG CCAAGAGTTC CATGGGGGAG GCCACATGGA GCAGCTGGCT TAGGAGGCGG 
GATTATCTCC TCGCTGCAGA AGATTGGGGA GTATCACCAG AGCCCCCTAC AGAACCCAGT 
ACCCCTCCAG GGCCTCCCTC TCAGCCAGTG GTCACTGAGA TCACCAAGAA CAGCATTACC 
CTGACCTGGA AGCCCAACCC GCAGGCGGGG GCCACAGTCA CCTCTTATGT GATAGAGGCC 
TTCAGCCAAG CAGCTGGCAA CACGTGGCGG ACAGTGGCAG ATGGTGTGCA GCTGGAGACA 
CACACGGTCA GTGGTCTGCA GCCCAACACC ATCTATCTGT TTCTGGTGCG AGCTGTGGGA 
GCCTGGGGCC TCAGTGAGCC CAGCCCCGTC TCTGAGCCCA TCCGCACCCA GGACAGCAAC 
CCATCCAGGC CAGTGGAGGA CCCATGGAGA GGCCAGCGGG GACTGGCTGA AGTGGCTGTG 
CATGTGCAGG AGCCCGTAGT CCTTGGGCTC CGGACCTTGC AGGTGTCCTG GACTGTAGAT 
GGCCCAGTCC AGCTGGTGCA AGGTTTCCGG GTGTCTTGGA GGGTAGCAGG TCCTGATGGG 
GGAAGCTGGA CAGTACTGGA CCTACAGTCC CCAAGCCAGC AAAGTACTGT GCTAAGAGGA 
CTCCCCCCAG GGACCCAAAT TCAGATCAAA GTGCAAGCCC AAGGCCAGGA GGGGCTGGGG 
CCTGAAAGTC CCTTTGTGAC CAGGAGCATT CCGGAGGAAG CCCCCAGTGG CCCCCCCCAG 
GGAGTGACAG TGGCCTTGGG GGGTGAAGGC AACAGCAGTG TCACGGTGTC CTGGGAACCT 
CCCCTCCCCT CCCAGCAAAA TGGGGTGATC AAGGAATACC AGATCTGGTG CCTGGGCAAT 
GAGAGCCGCT TCCACCTCAA CCGGTCTGCG GCAGGCTGGG CACGCTCCGC CGTGCTCCGG 
GGACTGCTGC CCGGCCTTCT CTACCGGACC CGGGTCGCGG CGGCCACCAG CGCCGGCGTG 
GGCGTGGCCA GCGTCCCGGT GTCGGTGCAG CTGCCCTCCC CGCCGGAGTC GGAGCCCGGG 
CTCGAGGTGC GCCCGGGGCT GGCGGAGCGC CTGGCCAGGG TGCTGCGGGC GCCTGCCTTC 
CTGGCGGGCA GCGGGGCCGC CTGCGGGGCG CTGCTGCTCG CGCTCTGCGC CGTCCTCTAC 
CGGCGCCGGA GGCAGCGCAA GGAGCTCAGT CACTACACGG CCTCCTTTGC CTACACACCG 
GCAGTGTCCT TCCCACACTC AGAGGGCCTC TCTGGAGCCA GTTCCAGGGG CTCACCTGGC 
CCCCGCCACC CTTCTCTCCA CCTCCTGGGG CAAACGGAAG GGAGCCGAGG ATTGCCAGTC 
TTCCTGAGGT TGGTCAGTCC TTGGGGAGGA TATGAGTTGG GGGAGCTTCT CTCATGCCTA 
ACCTTCACCC CGGCTTGGGA AGTAAGGTTT GGGGAGGGGG GAAGAAGCTG GTCCGGTGGG 
ATCTCCTTTG GCCCTCCATC CTCCTGTTGT CCGCTATTTC TCTGTCTCTG CCCTCTGCCC 
CCTGCCTCCC CCCCTGGCCT CCCTCTTTCT CCTCCTCAGA AGCAGGGATC TCCCTGTACC 
TGGCTCAGAC GGCCCGAGGC ACTGCCGCCC CTACAGAGGG TCCTGTCTAC AGCACCATTG 
ACCCAGCTGG GGAGGAGCTG CAGACCTTCC ACGGGGGGTT CCCCCCAAAT CCCTCGGGGG 
ACCCAGGCGC CTGGAGCCAA TATGCTCCTC CAGAATGGAG CCAGGGGGAC AGTGGAGCCA 
GGGGAGGCAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ROBO3
Entrez Gene ID	489307
Full Name	roundabout guidance receptor 3
General protein information	Preferred Names roundabout guidance receptor 3 Names roundabout homolog 3 roundabout, axon guidance receptor, homolog 3
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Pan troglodytes (chimpanzee)	ROBO3	XP_003313454.2
Canis lupus familiaris (dog)	ROBO3	XP_005619673.1
Danio rerio (zebrafish)	robo3	NP_571557.1
	ROBO3	XP_001106913.2
Mus musculus (house mouse)	Robo3	NP_001158239.1
Bos taurus (cattle)	ROBO3	XP_002699249.1
Rattus norvegicus (Norway rat)	Robo3	NP_001101605.1
Gallus gallus (chicken)	ROBO3	XP_003642645.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010970	XP_309724.3
Homo sapiens (human)	ROBO3	NP_071765.2
Xenopus tropicalis (tropical clawed frog)	robo3	XP_002937567.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ROBO3 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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