LOC489235 cDNA ORF clone, Canis lupus familiaris(dog)

The following LOC489235 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC489235 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe287737 XM_022417923.1
Latest version!
Canis lupus familiaris sperm flagellar protein 2 (LOC489235), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe287738 XM_022417924.1
Latest version!
Canis lupus familiaris sperm flagellar protein 2 (LOC489235), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe287739 XM_022417927.1
Latest version!
Canis lupus familiaris sperm flagellar protein 2 (LOC489235), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe287739 XM_022417925.1
Latest version!
Canis lupus familiaris sperm flagellar protein 2 (LOC489235), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe287739 XM_022417928.1
Latest version!
Canis lupus familiaris sperm flagellar protein 2 (LOC489235), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe287739 XM_022417926.1
Latest version!
Canis lupus familiaris sperm flagellar protein 2 (LOC489235), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe287740 XM_022417930.1
Latest version!
Canis lupus familiaris sperm flagellar protein 2 (LOC489235), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe287740 XM_022417929.1
Latest version!
Canis lupus familiaris sperm flagellar protein 2 (LOC489235), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe36161 XM_014113142.1
Latest version!
Canis lupus familiaris sperm flagellar 2 (SPEF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe36162 XM_014113143.2
Latest version!
Canis lupus familiaris sperm flagellar protein 2 (LOC489235), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe36163 XM_014113144.1
Latest version!
Canis lupus familiaris sperm flagellar 2 (SPEF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe36164 XM_014113145.1
Latest version!
Canis lupus familiaris sperm flagellar 2 (SPEF2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe36165 XM_014113146.2
Latest version!
Canis lupus familiaris sperm flagellar protein 2 (LOC489235), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe05532 XM_546353.4
Latest version!
Canis lupus familiaris sperm flagellar protein 2 (LOC489235), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe36160 XM_014113141.1
Latest version!
Canis lupus familiaris sperm flagellar 2 (SPEF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
View Old Accession Versions >>
OCe36162 XM_014113143.1 Canis lupus familiaris sperm flagellar 2 (SPEF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe36165 XM_014113146.1 Canis lupus familiaris sperm flagellar 2 (SPEF2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe05532 XM_546353.3 Canis lupus familiaris sperm flagellar 2 (SPEF2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe287737
    Clone ID Related Accession (Same CDS sequence) XM_022417923.1
    Accession Version XM_022417923.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5256bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product sperm flagellar protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAGTCATG GGATGAAAGG TCCCAAGTCA TTTGCAAAGG CATTTTCCAG TGGCTATCTA 
    ATTGGAGAAA TTCTGCACAA GTTTGAACTT CAGGGTGATT TTTCGGAATT TTCAGAAAGC
    AGAGTTTCAA ATGCCAAACT TAATAATTTT TCTCGCTTGG AGCCGACACT TCACCTTCTG
    GGTGTGCAGT TTGATCAGAA TGTGGCCCAC AACATCATAA CGGAAAAGCC TGGGGCGGCA
    ACCAAACTCT TGTATCAGTT GTACATTGTT CTTCAGAAAA AGAAGAAAAG TGGACTGACT
    GGAATAGAAA TGCAAACTAT GCAACCCCTG GCAAACGTCA GATACCAAAG TATGAAAAGT
    GAGGCTTTTC GAGATCAACG CCTGAGCAGG AGACGACAAA ATGAAATAAT GGCCAAAATC
    CAAGCAGCTA TCATACAGAT TCCTAAACCT GCATCAAATC GTACTTTGAG AGCACTGGAG
    GCCCAAAAAA TGATGAAAAA GAAAAAAGAG GCAGAGGATG TGGCCAATGA GATTAAGAAG
    TTTGAAGCTT TAATAAAAAA GGATCTCCAG GCCAAAGAAA GTGCCTCCAA GACTTCTTTA
    GATACAGCAG GCCAGACAAC TACTGATTTG TTAAACACTT ATTCGGATGA TGACTACATT
    AAAAAAATCC AAAAGCGCCT TGAAGAAGAT GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA
    CGGCGGAGAC TATTAATGGA CCAGTTAATA GCCCACGAAG CACAAGAGGA GGCGTATCGG
    GAGGAGCAGC TGATTAACCG GCTGATGCGG CAGTCCCAGC AGGAGCGCAG GATCTCTGTG
    CAGCTCATGC ATGTTCGGCA TGAAAAGGAA GTTTTGTGGC AAAACAGAAT TTTCCGAGAA
    AAACAGTATG AGGAAAGACG ACTTAAAGAT TTCCAGGATG CTCTTGATCG AGAAGCGGCT
    TTGGCAAAAC AAGCCAAGAT TGATTTTCAG GAACAAGCCA TTAGAGAGAA GGAAATTCAT
    GAACAGATCA CTGTGGAAAG AGCTCAAGCT CGTTACAAAA AGCATTATAC AATATGTGCA
    GAAATTTTGG ATCAAATACT TGATCTTTCC ACTAAAGTGT CAGACTACCG GATGTTGACA
    AATAATTTGA TTCCATATAA GTTGATGCAT GATTGGAAGG AACTGTTTTT TAATGGAAAG
    CCCATATACA AGCAAGCCTC TATTAAGCAA CTACCAGCCA CGCCCTCTGC AGAACAAGTT
    ATAGAACTGG AGAAGAGGGA CTTGCTGGAT AGCAATGATT ATGAAGAATA TAAGAATATG
    GTTGGAGAGT GGGCTTTACC AGAAGAAATG GTTGATAATT TACCACCCTC CAACAATACC
    ATATTGGGTC ATGTGATTCA CAGATTAATT GAAAAATCTC ATCCTCCTCA AGTCAAATCA
    ACAGTACTAG AATTACCTTC ATTTGCTATT AAAGGGTGCT TAGTTGGAAA AACATTTAGT
    GGAAAAACTA CTGCTCTAAA GGCTCTACAA AAAGACTTTC CTATTCAGGT ACTTTCTATT
    GACACTCTTG TCCAAGAAGC AATCAAAGCA TTCCATGACA ATGAAAAAAT CAGTGAGGCT
    CTACCAATTG AGAAAGAAGC TGAAGAAAAG TCTCTACCAG TTCTGCAAGA GAAGAACAAA
    GAAAGCCAGG ATCTACAGAA TGCATTTTCA CCTCATCCAG TTTCAGAAGA AGCGTTACCA
    GAAACAGAAG ATAAAACTAT ACTTAGTACT GATACAAATA AAACTCCAAA AGCTGAAGAA
    GTCAAATCAA GTGATAGTTT CTCTGAACTG ACTGTGCGGG CTCAGCTTGG TGCAAAATCA
    GAAAAGATGC TGAAGAAAGG AAAGAGCATT TCTGACATGT TGCTAGTTAA TATCTTGGTA
    AATGCTATTA ATGAGGTACC TGTGGATCAA GGCTGGGTCT TGGATGGTTT TCCAATGACG
    TTAAACCAAG CAAAGCTTCT GGAGGAAGCT CTCACGGGCT ACAACAGAGA GGCCCTAGAA
    CTGGAAGAAA AAAAAACACA AATACCCACA TTGGCTGTTG ATGCTAGCAC CTCCAAAGAA
    GTGCCTCTTC CCCCTTCTGC ATTTGACTTT GTCATGTTAT TAGATATTTC AGATAATTCC
    TTGCTGGACC GCATGAATTA CACAATGGCT GAAGCATTTT CAAGTGAAAC TTCTCATGAA
    GACATCACTC AACGTGTAAC TGCTGAAAAG CAAGATATGA ATGAGGACCA GAATTTAAGA
    GACCAGATTC AGCACAGAAT TATAGGCTTC TTGGACAACT GGCCTTTGTT GGAGCAATGG
    TTTACAGAGC CAGAAAATAT TTTGATAAAT ATCAATGCTG AAGTAGATAA AGAGTCTTTA
    TGCAATAAAG TAAAAGAAAT GTTTATGACT GAAATAATGA AAAAAGAGAA TAAAGTTAAA
    AAGAAATTAG AAGAAAAGGA AGCTGAGAAA AAAGAAGTTC CCCTGGGTGA GCCTCCGCCT
    CCTCCTCCTC CCCCTCCCCC TCCTCCTCCT GAGCCAGAAA AAGAGAAGGA AACCCATCCT
    CAACGAGAGC ATTCAAAAAC TCCTACTGCA AAAGGGAAAC CACCAGCAGA ACCCTTGACT
    GGTAAGCCAG ACTCTCTTCA GGAAGGAAAA GGAAAGAAAG GGGACACTTC ACTCAAAAGA
    AAAGGTTCTC CTAAAGGAAA ATCACCAGGA GGGAAAGGAC CACTAAAGAA ATCACCTGCT
    GATTCTACAG ACATGTCACC TGTTCCCATA GTGCCACCAC CACCCAAGCC AGGATCAGAA
    GAATGGGTCT ACGTGAATGA ACCAATTCCT GAGGAAATGC CTTCATTTTT AGTGCCTTAC
    TGGGAACTAA TCGAAAATTC CTACATAAAC TCCATCAAAA CAGTACTCAG GCATCTGAGA
    GAAGAACAGC ATAGTGTGGT TGCTTACTTA TACGAGGTTA GAACAAATTT CCAGCAGTTT
    CTATGGCGTC CAGATCACAA GCAAGATTTT GTATCTCAAT GGCAGGCTGA TTTCAATGCT
    CTTCCTGATG ACCTGTGGGA TGATGAAGAA ACAAAGTCTG AACTACACCA AAGAGTAAAT
    GATTTAAGAG ACCGCCTGTG GGACATCTGT GATGCCCGGA AGGAAGAAGC AGAGCAGGAG
    CGTCAGGATA TCATTAACGA GAGCTGGTTG CAAGACTCAA TCGGAATAAC GATGAACCAT
    TTCTTCTCAC TAATGCAGAC AGAACTGAAC CGTTTCCAAG ATACAAAGAG ACTCCTTCAA
    GATTATTACA GGGGAATGGA ATGCAAAATT CCAATCGATG ACAGTAAGAG ATTTACTCGA
    ATCCCGTTGG TCCAGCTGGA TAGTAGTAGA GACATTTTGG AAAGCCAACT CAGAATTCCT
    CTAATTCCAC GAATATCCAT TTCCCCGGAT TCCGCTATGA CCAAACCAAA AACAAAGGCC
    ATACTAAAGG GCAAGATCGA TTACTCGCTG GAAAATGTGG AGTTAAACTT TGAGGCAGAT
    GAGAAGTTGG TGATGGACAC CTGGCAGCAG GCTTCTTTAG CAATATCTCA CATGGTGGCT
    GCTGAAATTC ATCAGAGGCT CATGGAAGAA GAAAAGGAAA ACCAACCAGC AGACACAAAA
    GAAAAATCTC CTCAGATGGG GACAAATAAA AAAGCCAAAG AGGAACCACC CAAGAAAGAA
    AAGGCAGACA AAAAAGCAAA AGGCAGATCC CCGCCGACAG CAGAAGCCAC GCCTGTGACA
    GTCACAGCAG AGGAGATTAC CCAAAAGGAG AAGAGAAACG AACTGATGCT CAAAATCAAG
    GAAGAGCACC TCGCTGCCCT GCAGTTTGAA GAGATAGCCA CACAGTTCCG GCTTGAACTG
    ATAAAGACAA AAGCATTGGC TTTCCTTGAA GATTTACTAA CAAAGGTGGT CGATGTATAT
    AAACTCTTGG AAAAGTGGCT TGGTGAGAGG TACTTGAATG AAATGGCCAG TGTGGAAAAA
    TTAACTGAGG TGGCCCGTTA TCACATTGAA ACATCAACAA AAATTCAGAA TGAACTTTAC
    CTAGACCAAG AAGATTTCTT CATTAATGGA GATCTGAAAG TCTTTCCAGA TCCCCCTCCC
    CCAGTGCGCC CCCCACCTGT GGAAAAGGAA GAAAATGGCA CCCTGACCAT CGAACAGCTT
    GACAGTCTTA AAGATCAGTT CTTAGATATT GCACCCAAAG GCATAATAGG ACATAAAGCA
    TTTACTGACA TTCTGCTGGA TCTGGTAACC CTGAACCTTG GAACAAACAA CTTACCTAGT
    AGTTGGATGC ACCTCACGCA ACCAGAATTG CAGGAGTTAA CATCTTTAGT AATAGTAAAT
    TCGGAGTTTG TGGACTGGCG GAAATTCCTA TTAGTAATCT CGATGCCTTG GCCCATCCCC
    ATGGAGGAAG AGCTCCTCGA AACCCTGCAG AGGTTCAAGG CGGTGGATGA AGAGCAGTTG
    GGCACCATCA CTTTTGAGCA GTATATGCAG GCTGGTCTGT GGTTTACAGG AGATGAATAT
    ATAAAAATTC CAGAAAATCC TCTTGAACCT CTGCCGTTCA ATAGGCAGGA GCATCTTATA
    GAGTTTTTCT TTAGGCTATT TGCTGACCAT GAGAAGGATC CACCTCAACT TGACTACACA
    CAGATGCTGC TCTATTTTGC TTGCCATCCA GATGCTATGG AAGGGGTCTA CAGGGCCCTC
    AGTGTGGCCA TAGGAACTCA TGTCTTCCAA CCAGTCGAAA CTATTGCTAT TATTGGAGAA
    AAGTCCTCCT CCTTGACTGA TGCAAGTCCA GTTGAGGAGT ATCCTGAACT CGAGGGAAAC
    TTTATGCATG AGGAAGGAGA ATTAAAAGAA GACAGGGAGG AAAAGGAAGT AGAAATCCCT
    GAGAATGCAA ACACTGAAAA GGTATCCCTG GAAAGACTAC TCAAAGTGTT CCAAGGAGGA
    AAAGAAGTAC AGGATGCTAA CAGATTCACC AGCCACCTAC AGATAGAGAA CACTTACTCA
    GAGAACTTCG TAAAAGTATT TCAAGACCTA GGTGCCAAGG ATCTGGAGCC AGTTGAAGTT
    GCTGTTCTCT TGAAGCATCC TTTTATTCAA GACCTGATTT CAAATTATTC AGACTATAAA
    ATCCCTGATA TTAAAATAAT TCTCCAAAGG AGTGAGCATG TACAAGGAAG TGATGGAGAA
    AGATCTCCTT CAAGACTTAC AGAGGAAAAG AAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273631.1
    CDS174..5429
    Translation

    Target ORF information:

    RefSeq Version XM_022417923.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris sperm flagellar protein 2 (LOC489235), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417923.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAGTCATG GGATGAAAGG TCCCAAGTCA TTTGCAAAGG CATTTTCCAG TGGCTATCTA 
    ATTGGAGAAA TTCTGCACAA GTTTGAACTT CAGGGTGATT TTTCGGAATT TTCAGAAAGC
    AGAGTTTCAA ATGCCAAACT TAATAATTTT TCTCGCTTGG AGCCGACACT TCACCTTCTG
    GGTGTGCAGT TTGATCAGAA TGTGGCCCAC AACATCATAA CGGAAAAGCC TGGGGCGGCA
    ACCAAACTCT TGTATCAGTT GTACATTGTT CTTCAGAAAA AGAAGAAAAG TGGACTGACT
    GGAATAGAAA TGCAAACTAT GCAACCCCTG GCAAACGTCA GATACCAAAG TATGAAAAGT
    GAGGCTTTTC GAGATCAACG CCTGAGCAGG AGACGACAAA ATGAAATAAT GGCCAAAATC
    CAAGCAGCTA TCATACAGAT TCCTAAACCT GCATCAAATC GTACTTTGAG AGCACTGGAG
    GCCCAAAAAA TGATGAAAAA GAAAAAAGAG GCAGAGGATG TGGCCAATGA GATTAAGAAG
    TTTGAAGCTT TAATAAAAAA GGATCTCCAG GCCAAAGAAA GTGCCTCCAA GACTTCTTTA
    GATACAGCAG GCCAGACAAC TACTGATTTG TTAAACACTT ATTCGGATGA TGACTACATT
    AAAAAAATCC AAAAGCGCCT TGAAGAAGAT GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA
    CGGCGGAGAC TATTAATGGA CCAGTTAATA GCCCACGAAG CACAAGAGGA GGCGTATCGG
    GAGGAGCAGC TGATTAACCG GCTGATGCGG CAGTCCCAGC AGGAGCGCAG GATCTCTGTG
    CAGCTCATGC ATGTTCGGCA TGAAAAGGAA GTTTTGTGGC AAAACAGAAT TTTCCGAGAA
    AAACAGTATG AGGAAAGACG ACTTAAAGAT TTCCAGGATG CTCTTGATCG AGAAGCGGCT
    TTGGCAAAAC AAGCCAAGAT TGATTTTCAG GAACAAGCCA TTAGAGAGAA GGAAATTCAT
    GAACAGATCA CTGTGGAAAG AGCTCAAGCT CGTTACAAAA AGCATTATAC AATATGTGCA
    GAAATTTTGG ATCAAATACT TGATCTTTCC ACTAAAGTGT CAGACTACCG GATGTTGACA
    AATAATTTGA TTCCATATAA GTTGATGCAT GATTGGAAGG AACTGTTTTT TAATGGAAAG
    CCCATATACA AGCAAGCCTC TATTAAGCAA CTACCAGCCA CGCCCTCTGC AGAACAAGTT
    ATAGAACTGG AGAAGAGGGA CTTGCTGGAT AGCAATGATT ATGAAGAATA TAAGAATATG
    GTTGGAGAGT GGGCTTTACC AGAAGAAATG GTTGATAATT TACCACCCTC CAACAATACC
    ATATTGGGTC ATGTGATTCA CAGATTAATT GAAAAATCTC ATCCTCCTCA AGTCAAATCA
    ACAGTACTAG AATTACCTTC ATTTGCTATT AAAGGGTGCT TAGTTGGAAA AACATTTAGT
    GGAAAAACTA CTGCTCTAAA GGCTCTACAA AAAGACTTTC CTATTCAGGT ACTTTCTATT
    GACACTCTTG TCCAAGAAGC AATCAAAGCA TTCCATGACA ATGAAAAAAT CAGTGAGGCT
    CTACCAATTG AGAAAGAAGC TGAAGAAAAG TCTCTACCAG TTCTGCAAGA GAAGAACAAA
    GAAAGCCAGG ATCTACAGAA TGCATTTTCA CCTCATCCAG TTTCAGAAGA AGCGTTACCA
    GAAACAGAAG ATAAAACTAT ACTTAGTACT GATACAAATA AAACTCCAAA AGCTGAAGAA
    GTCAAATCAA GTGATAGTTT CTCTGAACTG ACTGTGCGGG CTCAGCTTGG TGCAAAATCA
    GAAAAGATGC TGAAGAAAGG AAAGAGCATT TCTGACATGT TGCTAGTTAA TATCTTGGTA
    AATGCTATTA ATGAGGTACC TGTGGATCAA GGCTGGGTCT TGGATGGTTT TCCAATGACG
    TTAAACCAAG CAAAGCTTCT GGAGGAAGCT CTCACGGGCT ACAACAGAGA GGCCCTAGAA
    CTGGAAGAAA AAAAAACACA AATACCCACA TTGGCTGTTG ATGCTAGCAC CTCCAAAGAA
    GTGCCTCTTC CCCCTTCTGC ATTTGACTTT GTCATGTTAT TAGATATTTC AGATAATTCC
    TTGCTGGACC GCATGAATTA CACAATGGCT GAAGCATTTT CAAGTGAAAC TTCTCATGAA
    GACATCACTC AACGTGTAAC TGCTGAAAAG CAAGATATGA ATGAGGACCA GAATTTAAGA
    GACCAGATTC AGCACAGAAT TATAGGCTTC TTGGACAACT GGCCTTTGTT GGAGCAATGG
    TTTACAGAGC CAGAAAATAT TTTGATAAAT ATCAATGCTG AAGTAGATAA AGAGTCTTTA
    TGCAATAAAG TAAAAGAAAT GTTTATGACT GAAATAATGA AAAAAGAGAA TAAAGTTAAA
    AAGAAATTAG AAGAAAAGGA AGCTGAGAAA AAAGAAGTTC CCCTGGGTGA GCCTCCGCCT
    CCTCCTCCTC CCCCTCCCCC TCCTCCTCCT GAGCCAGAAA AAGAGAAGGA AACCCATCCT
    CAACGAGAGC ATTCAAAAAC TCCTACTGCA AAAGGGAAAC CACCAGCAGA ACCCTTGACT
    GGTAAGCCAG ACTCTCTTCA GGAAGGAAAA GGAAAGAAAG GGGACACTTC ACTCAAAAGA
    AAAGGTTCTC CTAAAGGAAA ATCACCAGGA GGGAAAGGAC CACTAAAGAA ATCACCTGCT
    GATTCTACAG ACATGTCACC TGTTCCCATA GTGCCACCAC CACCCAAGCC AGGATCAGAA
    GAATGGGTCT ACGTGAATGA ACCAATTCCT GAGGAAATGC CTTCATTTTT AGTGCCTTAC
    TGGGAACTAA TCGAAAATTC CTACATAAAC TCCATCAAAA CAGTACTCAG GCATCTGAGA
    GAAGAACAGC ATAGTGTGGT TGCTTACTTA TACGAGGTTA GAACAAATTT CCAGCAGTTT
    CTATGGCGTC CAGATCACAA GCAAGATTTT GTATCTCAAT GGCAGGCTGA TTTCAATGCT
    CTTCCTGATG ACCTGTGGGA TGATGAAGAA ACAAAGTCTG AACTACACCA AAGAGTAAAT
    GATTTAAGAG ACCGCCTGTG GGACATCTGT GATGCCCGGA AGGAAGAAGC AGAGCAGGAG
    CGTCAGGATA TCATTAACGA GAGCTGGTTG CAAGACTCAA TCGGAATAAC GATGAACCAT
    TTCTTCTCAC TAATGCAGAC AGAACTGAAC CGTTTCCAAG ATACAAAGAG ACTCCTTCAA
    GATTATTACA GGGGAATGGA ATGCAAAATT CCAATCGATG ACAGTAAGAG ATTTACTCGA
    ATCCCGTTGG TCCAGCTGGA TAGTAGTAGA GACATTTTGG AAAGCCAACT CAGAATTCCT
    CTAATTCCAC GAATATCCAT TTCCCCGGAT TCCGCTATGA CCAAACCAAA AACAAAGGCC
    ATACTAAAGG GCAAGATCGA TTACTCGCTG GAAAATGTGG AGTTAAACTT TGAGGCAGAT
    GAGAAGTTGG TGATGGACAC CTGGCAGCAG GCTTCTTTAG CAATATCTCA CATGGTGGCT
    GCTGAAATTC ATCAGAGGCT CATGGAAGAA GAAAAGGAAA ACCAACCAGC AGACACAAAA
    GAAAAATCTC CTCAGATGGG GACAAATAAA AAAGCCAAAG AGGAACCACC CAAGAAAGAA
    AAGGCAGACA AAAAAGCAAA AGGCAGATCC CCGCCGACAG CAGAAGCCAC GCCTGTGACA
    GTCACAGCAG AGGAGATTAC CCAAAAGGAG AAGAGAAACG AACTGATGCT CAAAATCAAG
    GAAGAGCACC TCGCTGCCCT GCAGTTTGAA GAGATAGCCA CACAGTTCCG GCTTGAACTG
    ATAAAGACAA AAGCATTGGC TTTCCTTGAA GATTTACTAA CAAAGGTGGT CGATGTATAT
    AAACTCTTGG AAAAGTGGCT TGGTGAGAGG TACTTGAATG AAATGGCCAG TGTGGAAAAA
    TTAACTGAGG TGGCCCGTTA TCACATTGAA ACATCAACAA AAATTCAGAA TGAACTTTAC
    CTAGACCAAG AAGATTTCTT CATTAATGGA GATCTGAAAG TCTTTCCAGA TCCCCCTCCC
    CCAGTGCGCC CCCCACCTGT GGAAAAGGAA GAAAATGGCA CCCTGACCAT CGAACAGCTT
    GACAGTCTTA AAGATCAGTT CTTAGATATT GCACCCAAAG GCATAATAGG ACATAAAGCA
    TTTACTGACA TTCTGCTGGA TCTGGTAACC CTGAACCTTG GAACAAACAA CTTACCTAGT
    AGTTGGATGC ACCTCACGCA ACCAGAATTG CAGGAGTTAA CATCTTTAGT AATAGTAAAT
    TCGGAGTTTG TGGACTGGCG GAAATTCCTA TTAGTAATCT CGATGCCTTG GCCCATCCCC
    ATGGAGGAAG AGCTCCTCGA AACCCTGCAG AGGTTCAAGG CGGTGGATGA AGAGCAGTTG
    GGCACCATCA CTTTTGAGCA GTATATGCAG GCTGGTCTGT GGTTTACAGG AGATGAATAT
    ATAAAAATTC CAGAAAATCC TCTTGAACCT CTGCCGTTCA ATAGGCAGGA GCATCTTATA
    GAGTTTTTCT TTAGGCTATT TGCTGACCAT GAGAAGGATC CACCTCAACT TGACTACACA
    CAGATGCTGC TCTATTTTGC TTGCCATCCA GATGCTATGG AAGGGGTCTA CAGGGCCCTC
    AGTGTGGCCA TAGGAACTCA TGTCTTCCAA CCAGTCGAAA CTATTGCTAT TATTGGAGAA
    AAGTCCTCCT CCTTGACTGA TGCAAGTCCA GTTGAGGAGT ATCCTGAACT CGAGGGAAAC
    TTTATGCATG AGGAAGGAGA ATTAAAAGAA GACAGGGAGG AAAAGGAAGT AGAAATCCCT
    GAGAATGCAA ACACTGAAAA GGTATCCCTG GAAAGACTAC TCAAAGTGTT CCAAGGAGGA
    AAAGAAGTAC AGGATGCTAA CAGATTCACC AGCCACCTAC AGATAGAGAA CACTTACTCA
    GAGAACTTCG TAAAAGTATT TCAAGACCTA GGTGCCAAGG ATCTGGAGCC AGTTGAAGTT
    GCTGTTCTCT TGAAGCATCC TTTTATTCAA GACCTGATTT CAAATTATTC AGACTATAAA
    ATCCCTGATA TTAAAATAAT TCTCCAAAGG AGTGAGCATG TACAAGGAAG TGATGGAGAA
    AGATCTCCTT CAAGACTTAC AGAGGAAAAG AAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287738
    Clone ID Related Accession (Same CDS sequence) XM_022417924.1
    Accession Version XM_022417924.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product sperm flagellar protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCGGAGA TTCTGTGCCA GTGGCTCAAC CAGGAGGTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAAT TCTGCACAAG
    TTTGAACTTC AGGGTGATTT TTCGGAATTT TCAGAAAGCA GAGTTTCAAA TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCGACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCACA ACATCATAAC GGAAAAGCCT GGGGCGGCAA CCAAACTCTT GTATCAGTTG
    TACATTGTTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAATAGAAAT GCAAACTATG
    CAACCCCTGG CAAACGTCAG ATACCAAAGT ATGAAAAGTG AGGCTTTTCG AGATCAACGC
    CTGAGCAGGA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCTG CATCAAATCG TACTTTGAGA GCACTGGAGG CCCAAAAAAT GATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAG ATTAAGAAGT TTGAAGCTTT AATAAAAAAG
    GATCTCCAGG CCAAAGAAAG TGCCTCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACT
    ACTGATTTGT TAAACACTTA TTCGGATGAT GACTACATTA AAAAAATCCA AAAGCGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT ATTAATGGAC
    CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCGTATCGGG AGGAGCAGCT GATTAACCGG
    CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATCTCTGTGC AGCTCATGCA TGTTCGGCAT
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCCGAGAAA AACAGTATGA GGAAAGACGA
    CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT
    GATTTTCAGG AACAAGCCAT TAGAGAGAAG GAAATTCATG AACAGATCAC TGTGGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATACA ATATGTGCAG AAATTTTGGA TCAAATACTT
    GATCTTTCCA CTAAAGTGTC AGACTACCGG ATGTTGACAA ATAATTTGAT TCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT AATGGAAAGC CCATATACAA GCAAGCCTCT
    ATTAAGCAAC TACCAGCCAC GCCCTCTGCA GAACAAGTTA TAGAACTGGA GAAGAGGGAC
    TTGCTGGATA GCAATGATTA TGAAGAATAT AAGAATATGG TTGGAGAGTG GGCTTTACCA
    GAAGAAATGG TTGATAATTT ACCACCCTCC AACAATACCA TATTGGGTCA TGTGATTCAC
    AGATTAATTG AAAAATCTCA TCCTCCTCAA GTCAAATCAA CAGTACTAGA ATTACCTTCA
    TTTGCTATTA AAGGGTGCTT AGTTGGAAAA ACATTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTACAAA AAGACTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT CCAAGAAGCA
    ATCAAAGCAT TCCATGACAA TGAAAAAATC AGTGAGGCTC TACCAATTGA GAAAGAAGCT
    GAAGAAAAGT CTCTACCAGT TCTGCAAGAG AAGAACAAAG AAAGCCAGGA TCTACAGAAT
    GCATTTTCAC CTCATCCAGT TTCAGAAGAA GCGTTACCAG AAACAGAAGA TAAAACTATA
    CTTAGTACTG ATACAAATAA AACTCCAAAA GCTGAAGAAG TCAAATCAAG TGATAGTTTC
    TCTGAACTGA CTGTGCGGGC TCAGCTTGGT GCAAAATCAG AAAAGATGCT GAAGAAAGGA
    AAGAGCATTT CTGACATGTT GCTAGTTAAT ATCTTGGTAA ATGCTATTAA TGAGGTACCT
    GTGGATCAAG GCTGGGTCTT GGATGGTTTT CCAATGACGT TAAACCAAGC AAAGCTTCTG
    GAGGAAGCTC TCACGGGCTA CAACAGAGAG GCCCTAGAAC TGGAAGAAAA AAAAACACAA
    ATACCCACAT TGGCTGTTGA TGCTAGCACC TCCAAAGAAG TGCCTCTTCC CCCTTCTGCA
    TTTGACTTTG TCATGTTATT AGATATTTCA GATAATTCCT TGCTGGACCG CATGAATTAC
    ACAATGGCTG AAGCATTTTC AAGTGAAACT TCTCATGAAG ACATCACTCA ACGTGTAACT
    GCTGAAAAGC AAGATATGAA TGAGGACCAG AATTTAAGAG ACCAGATTCA GCACAGAATT
    ATAGGCTTCT TGGACAACTG GCCTTTGTTG GAGCAATGGT TTACAGAGCC AGAAAATATT
    TTGATAAATA TCAATGCTGA AGTAGATAAA GAGTCTTTAT GCAATAAAGT AAAAGAAATG
    TTTATGACTG AAATAATGAA AAAAGAGAAT AAAGTTAAAA AGAAATTAGA AGAAAAGGAA
    GCTGAGAAAA AAGAAGTTCC CCTGGGTGAG CCTCCGCCTC CTCCTCCTCC CCCTCCCCCT
    CCTCCTCCTG AGCCAGAAAA AGAGAAGGAA ACCCATCCTC AACGAGAGCA TTCAAAAACT
    CCTACTGCAA AAGGGAAACC ACCAGCAGAA CCCTTGACTG GTAAGCCAGA CTCTCTTCAG
    GAAGGAAAAG GAAAGAAAGG GGACACTTCA CTCAAAAGAA AAGGTTCTCC TAAAGGAAAA
    TCACCAGGAG GGAAAGGACC ACTAAAGAAA TCACCTGCTG ATTCTACAGA CATGTCACCT
    GTTCCCATAG TGCCACCACC ACCCAAGCCA GGATCAGAAG AATGGGTCTA CGTGAATGAA
    CCAATTCCTG AGGAAATGCC TTCATTTTTA GTGCCTTACT GGGAACTAAT CGAAAATTCC
    TACATAAACT CCATCAAAAC AGTACTCAGG CATCTGAGAG AAGAACAGCA TAGTGTGGTT
    GCTTACTTAT ACGAGGTTAG AACAAATTTC CAGCAGTTTC TATGGCGTCC AGATCACAAG
    CAAGATTTTG TATCTCAATG GCAGGCTGAT TTCAATGCTC TTCCTGATGA CCTGTGGGAT
    GATGAAGAAA CAAAGTCTGA ACTACACCAA AGAGTAAATG ATTTAAGAGA CCGCCTGTGG
    GACATCTGTG ATGCCCGGAA GGAAGAAGCA GAGCAGGAGC GTCAGGATAT CATTAACGAG
    AGCTGGTTGC AAGACTCAAT CGGAATAACG ATGAACCATT TCTTCTCACT AATGCAGACA
    GAACTGAACC GTTTCCAAGA TACAAAGAGA CTCCTTCAAG ATTATTACAG GGGAATGGAA
    TGCAAAATTC CAATCGATGA CAGTAAGAGA TTTACTCGAA TCCCGTTGGT CCAGCTGGAT
    AGTAGTAGAG ACATTTTGGA AAGCCAACTC AGAATTCCTC TAATTCCACG AATATCCATT
    TCCCCGGATT CCGCTATGAC CAAACCAAAA ACAAAGGCCA TACTAAAGGG CAAGATCGAT
    TACTCGCTGG AAAATGTGGA GTTAAACTTT GAGGCAGATG AGAAGTTGGT GATGGACACC
    TGGCAGCAGG CTTCTTTAGC AATATCTCAC ATGGTGGCTG CTGAAATTCA TCAGAGGCTC
    ATGGAAGAAG AAAAGGAAAA CCAACCAGCA GACACAAAAG AAAAATCTCC TCAGATGGGG
    ACAAATAAAA AAGCCAAAGA GGAACCACCC AAGAAAGAAA AGGCAGACAA AAAAGCAAAA
    GGCAGATCCC CGCCGACAGC AGAAGCCACG CCTGTGACAG TCACAGCAGA GGAGATTACC
    CAAAAGGAGA AGAGAAACGA ACTGATGCTC AAAATCAAGG AAGAGCACCT CGCTGCCCTG
    CAGTTTGAAG AGATAGCCAC ACAGTTCCGG CTTGAACTGA TAAAGACAAA AGCATTGGCT
    TTCCTTGAAG ATTTACTAAC AAAGGTGGTC GATGTATATA AACTCTTGGA AAAGTGGCTT
    GGTGAGAGGT ACTTGAATGA AATGGCCAGT GTGGAAAAAT TAACTGAGGT GGCCCGTTAT
    CACATTGAAA CATCAACAAA AATTCAGAAT GAACTTTACC TAGACCAAGA AGATTTCTTC
    ATTAATGGAG ATCTGAAAGT CTTTCCAGAT CCCCCTCCCC CAGTGCGCCC CCCACCTGTG
    GAAAAGGAAG AAAATGGCAC CCTGACCATC GAACAGCTTG ACAGTCTTAA AGATCAGTTC
    TTAGATATTG CACCCAAAGG CATAATAGGA CATAAAGCAT TTACTGACAT TCTGCTGGAT
    CTGGTAACCC TGAACCTTGG AACAAACAAC TTACCTAGTA GTTGGATGCA CCTCACGCAA
    CCAGAAGCTG GTCTGTGGTT TACAGGAGAT GAATATATAA AAATTCCAGA AAATCCTCTT
    GAACCTCTGC CGTTCAATAG GCAGGAGCAT CTTATAGAGT TTTTCTTTAG GCTATTTGCT
    GACCATGAGA AGGATCCACC TCAACTTGAC TACACACAGA TGCTGCTCTA TTTTGCTTGC
    CATCCAGATG CTATGGAAGG GGTCTACAGG GCCCTCAGTG TGGCCATAGG AACTCATGTC
    TTCCAACCAG TCGAAACTAT TGCTATTATT GGAGAAAAGT CCTCCTCCTT GACTGATGCA
    AGTCCAGTTG AGGAGTATCC TGAACTCGAG GGAAACTTTA TGCATGAGGA AGGAGAATTA
    AAAGAAGACA GGGAGGAAAA GGAAGTAGAA ATCCCTGAGA ATGCAAACAC TGAAAAGGTA
    TCCCTGGAAA GACTACTCAA AGTGTTCCAA GGAGGAAAAG AAGTACAGGA TGCTAACAGA
    TTCACCAGCC ACCTACAGAT AGAGAACACT TACTCAGAGA ACTTCGTAAA AGTATTTCAA
    GACCTAGGTG CCAAGGATCT GGAGCCAGTT GAAGTTGCTG TTCTCTTGAA GCATCCTTTT
    ATTCAAGACC TGATTTCAAA TTATTCAGAC TATAAAATCC CTGATATTAA AATAATTCTC
    CAAAGGAGTG AGCATGTACA AGGAAGTGAT GGAGAAAGAT CTCCTTCAAG ACTTACAGAG
    GAAAAGAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273632.1
    CDS159..5270
    Translation

    Target ORF information:

    RefSeq Version XM_022417924.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris sperm flagellar protein 2 (LOC489235), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417924.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCGGAGA TTCTGTGCCA GTGGCTCAAC CAGGAGGTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAAT TCTGCACAAG
    TTTGAACTTC AGGGTGATTT TTCGGAATTT TCAGAAAGCA GAGTTTCAAA TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCGACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCACA ACATCATAAC GGAAAAGCCT GGGGCGGCAA CCAAACTCTT GTATCAGTTG
    TACATTGTTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAATAGAAAT GCAAACTATG
    CAACCCCTGG CAAACGTCAG ATACCAAAGT ATGAAAAGTG AGGCTTTTCG AGATCAACGC
    CTGAGCAGGA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCTG CATCAAATCG TACTTTGAGA GCACTGGAGG CCCAAAAAAT GATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAG ATTAAGAAGT TTGAAGCTTT AATAAAAAAG
    GATCTCCAGG CCAAAGAAAG TGCCTCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACT
    ACTGATTTGT TAAACACTTA TTCGGATGAT GACTACATTA AAAAAATCCA AAAGCGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT ATTAATGGAC
    CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCGTATCGGG AGGAGCAGCT GATTAACCGG
    CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATCTCTGTGC AGCTCATGCA TGTTCGGCAT
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCCGAGAAA AACAGTATGA GGAAAGACGA
    CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT
    GATTTTCAGG AACAAGCCAT TAGAGAGAAG GAAATTCATG AACAGATCAC TGTGGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATACA ATATGTGCAG AAATTTTGGA TCAAATACTT
    GATCTTTCCA CTAAAGTGTC AGACTACCGG ATGTTGACAA ATAATTTGAT TCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT AATGGAAAGC CCATATACAA GCAAGCCTCT
    ATTAAGCAAC TACCAGCCAC GCCCTCTGCA GAACAAGTTA TAGAACTGGA GAAGAGGGAC
    TTGCTGGATA GCAATGATTA TGAAGAATAT AAGAATATGG TTGGAGAGTG GGCTTTACCA
    GAAGAAATGG TTGATAATTT ACCACCCTCC AACAATACCA TATTGGGTCA TGTGATTCAC
    AGATTAATTG AAAAATCTCA TCCTCCTCAA GTCAAATCAA CAGTACTAGA ATTACCTTCA
    TTTGCTATTA AAGGGTGCTT AGTTGGAAAA ACATTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTACAAA AAGACTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT CCAAGAAGCA
    ATCAAAGCAT TCCATGACAA TGAAAAAATC AGTGAGGCTC TACCAATTGA GAAAGAAGCT
    GAAGAAAAGT CTCTACCAGT TCTGCAAGAG AAGAACAAAG AAAGCCAGGA TCTACAGAAT
    GCATTTTCAC CTCATCCAGT TTCAGAAGAA GCGTTACCAG AAACAGAAGA TAAAACTATA
    CTTAGTACTG ATACAAATAA AACTCCAAAA GCTGAAGAAG TCAAATCAAG TGATAGTTTC
    TCTGAACTGA CTGTGCGGGC TCAGCTTGGT GCAAAATCAG AAAAGATGCT GAAGAAAGGA
    AAGAGCATTT CTGACATGTT GCTAGTTAAT ATCTTGGTAA ATGCTATTAA TGAGGTACCT
    GTGGATCAAG GCTGGGTCTT GGATGGTTTT CCAATGACGT TAAACCAAGC AAAGCTTCTG
    GAGGAAGCTC TCACGGGCTA CAACAGAGAG GCCCTAGAAC TGGAAGAAAA AAAAACACAA
    ATACCCACAT TGGCTGTTGA TGCTAGCACC TCCAAAGAAG TGCCTCTTCC CCCTTCTGCA
    TTTGACTTTG TCATGTTATT AGATATTTCA GATAATTCCT TGCTGGACCG CATGAATTAC
    ACAATGGCTG AAGCATTTTC AAGTGAAACT TCTCATGAAG ACATCACTCA ACGTGTAACT
    GCTGAAAAGC AAGATATGAA TGAGGACCAG AATTTAAGAG ACCAGATTCA GCACAGAATT
    ATAGGCTTCT TGGACAACTG GCCTTTGTTG GAGCAATGGT TTACAGAGCC AGAAAATATT
    TTGATAAATA TCAATGCTGA AGTAGATAAA GAGTCTTTAT GCAATAAAGT AAAAGAAATG
    TTTATGACTG AAATAATGAA AAAAGAGAAT AAAGTTAAAA AGAAATTAGA AGAAAAGGAA
    GCTGAGAAAA AAGAAGTTCC CCTGGGTGAG CCTCCGCCTC CTCCTCCTCC CCCTCCCCCT
    CCTCCTCCTG AGCCAGAAAA AGAGAAGGAA ACCCATCCTC AACGAGAGCA TTCAAAAACT
    CCTACTGCAA AAGGGAAACC ACCAGCAGAA CCCTTGACTG GTAAGCCAGA CTCTCTTCAG
    GAAGGAAAAG GAAAGAAAGG GGACACTTCA CTCAAAAGAA AAGGTTCTCC TAAAGGAAAA
    TCACCAGGAG GGAAAGGACC ACTAAAGAAA TCACCTGCTG ATTCTACAGA CATGTCACCT
    GTTCCCATAG TGCCACCACC ACCCAAGCCA GGATCAGAAG AATGGGTCTA CGTGAATGAA
    CCAATTCCTG AGGAAATGCC TTCATTTTTA GTGCCTTACT GGGAACTAAT CGAAAATTCC
    TACATAAACT CCATCAAAAC AGTACTCAGG CATCTGAGAG AAGAACAGCA TAGTGTGGTT
    GCTTACTTAT ACGAGGTTAG AACAAATTTC CAGCAGTTTC TATGGCGTCC AGATCACAAG
    CAAGATTTTG TATCTCAATG GCAGGCTGAT TTCAATGCTC TTCCTGATGA CCTGTGGGAT
    GATGAAGAAA CAAAGTCTGA ACTACACCAA AGAGTAAATG ATTTAAGAGA CCGCCTGTGG
    GACATCTGTG ATGCCCGGAA GGAAGAAGCA GAGCAGGAGC GTCAGGATAT CATTAACGAG
    AGCTGGTTGC AAGACTCAAT CGGAATAACG ATGAACCATT TCTTCTCACT AATGCAGACA
    GAACTGAACC GTTTCCAAGA TACAAAGAGA CTCCTTCAAG ATTATTACAG GGGAATGGAA
    TGCAAAATTC CAATCGATGA CAGTAAGAGA TTTACTCGAA TCCCGTTGGT CCAGCTGGAT
    AGTAGTAGAG ACATTTTGGA AAGCCAACTC AGAATTCCTC TAATTCCACG AATATCCATT
    TCCCCGGATT CCGCTATGAC CAAACCAAAA ACAAAGGCCA TACTAAAGGG CAAGATCGAT
    TACTCGCTGG AAAATGTGGA GTTAAACTTT GAGGCAGATG AGAAGTTGGT GATGGACACC
    TGGCAGCAGG CTTCTTTAGC AATATCTCAC ATGGTGGCTG CTGAAATTCA TCAGAGGCTC
    ATGGAAGAAG AAAAGGAAAA CCAACCAGCA GACACAAAAG AAAAATCTCC TCAGATGGGG
    ACAAATAAAA AAGCCAAAGA GGAACCACCC AAGAAAGAAA AGGCAGACAA AAAAGCAAAA
    GGCAGATCCC CGCCGACAGC AGAAGCCACG CCTGTGACAG TCACAGCAGA GGAGATTACC
    CAAAAGGAGA AGAGAAACGA ACTGATGCTC AAAATCAAGG AAGAGCACCT CGCTGCCCTG
    CAGTTTGAAG AGATAGCCAC ACAGTTCCGG CTTGAACTGA TAAAGACAAA AGCATTGGCT
    TTCCTTGAAG ATTTACTAAC AAAGGTGGTC GATGTATATA AACTCTTGGA AAAGTGGCTT
    GGTGAGAGGT ACTTGAATGA AATGGCCAGT GTGGAAAAAT TAACTGAGGT GGCCCGTTAT
    CACATTGAAA CATCAACAAA AATTCAGAAT GAACTTTACC TAGACCAAGA AGATTTCTTC
    ATTAATGGAG ATCTGAAAGT CTTTCCAGAT CCCCCTCCCC CAGTGCGCCC CCCACCTGTG
    GAAAAGGAAG AAAATGGCAC CCTGACCATC GAACAGCTTG ACAGTCTTAA AGATCAGTTC
    TTAGATATTG CACCCAAAGG CATAATAGGA CATAAAGCAT TTACTGACAT TCTGCTGGAT
    CTGGTAACCC TGAACCTTGG AACAAACAAC TTACCTAGTA GTTGGATGCA CCTCACGCAA
    CCAGAAGCTG GTCTGTGGTT TACAGGAGAT GAATATATAA AAATTCCAGA AAATCCTCTT
    GAACCTCTGC CGTTCAATAG GCAGGAGCAT CTTATAGAGT TTTTCTTTAG GCTATTTGCT
    GACCATGAGA AGGATCCACC TCAACTTGAC TACACACAGA TGCTGCTCTA TTTTGCTTGC
    CATCCAGATG CTATGGAAGG GGTCTACAGG GCCCTCAGTG TGGCCATAGG AACTCATGTC
    TTCCAACCAG TCGAAACTAT TGCTATTATT GGAGAAAAGT CCTCCTCCTT GACTGATGCA
    AGTCCAGTTG AGGAGTATCC TGAACTCGAG GGAAACTTTA TGCATGAGGA AGGAGAATTA
    AAAGAAGACA GGGAGGAAAA GGAAGTAGAA ATCCCTGAGA ATGCAAACAC TGAAAAGGTA
    TCCCTGGAAA GACTACTCAA AGTGTTCCAA GGAGGAAAAG AAGTACAGGA TGCTAACAGA
    TTCACCAGCC ACCTACAGAT AGAGAACACT TACTCAGAGA ACTTCGTAAA AGTATTTCAA
    GACCTAGGTG CCAAGGATCT GGAGCCAGTT GAAGTTGCTG TTCTCTTGAA GCATCCTTTT
    ATTCAAGACC TGATTTCAAA TTATTCAGAC TATAAAATCC CTGATATTAA AATAATTCTC
    CAAAGGAGTG AGCATGTACA AGGAAGTGAT GGAGAAAGAT CTCCTTCAAG ACTTACAGAG
    GAAAAGAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287739
    Clone ID Related Accession (Same CDS sequence) XM_022417925.1 , XM_022417927.1 , XM_022417928.1 , XM_022417926.1
    Accession Version XM_022417925.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product sperm flagellar protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCAAACTA TGCAACCCCT GGCAAACGTC AGATACCAAA GTATGAAAAG TGAGGCTTTT 
    CGAGATCAAC GCCTGAGCAG GAGACGACAA AATGAAATAA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC TGCATCAAAT CGTACTTTGA GAGCACTGGA GGCCCAAAAA
    ATGATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AGATTAAGAA GTTTGAAGCT
    TTAATAAAAA AGGATCTCCA GGCCAAAGAA AGTGCCTCCA AGACTTCTTT AGATACAGCA
    GGCCAGACAA CTACTGATTT GTTAAACACT TATTCGGATG ATGACTACAT TAAAAAAATC
    CAAAAGCGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTATTAATGG ACCAGTTAAT AGCCCACGAA GCACAAGAGG AGGCGTATCG GGAGGAGCAG
    CTGATTAACC GGCTGATGCG GCAGTCCCAG CAGGAGCGCA GGATCTCTGT GCAGCTCATG
    CATGTTCGGC ATGAAAAGGA AGTTTTGTGG CAAAACAGAA TTTTCCGAGA AAAACAGTAT
    GAGGAAAGAC GACTTAAAGA TTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTGGCAAAA
    CAAGCCAAGA TTGATTTTCA GGAACAAGCC ATTAGAGAGA AGGAAATTCA TGAACAGATC
    ACTGTGGAAA GAGCTCAAGC TCGTTACAAA AAGCATTATA CAATATGTGC AGAAATTTTG
    GATCAAATAC TTGATCTTTC CACTAAAGTG TCAGACTACC GGATGTTGAC AAATAATTTG
    ATTCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TTAATGGAAA GCCCATATAC
    AAGCAAGCCT CTATTAAGCA ACTACCAGCC ACGCCCTCTG CAGAACAAGT TATAGAACTG
    GAGAAGAGGG ACTTGCTGGA TAGCAATGAT TATGAAGAAT ATAAGAATAT GGTTGGAGAG
    TGGGCTTTAC CAGAAGAAAT GGTTGATAAT TTACCACCCT CCAACAATAC CATATTGGGT
    CATGTGATTC ACAGATTAAT TGAAAAATCT CATCCTCCTC AAGTCAAATC AACAGTACTA
    GAATTACCTT CATTTGCTAT TAAAGGGTGC TTAGTTGGAA AAACATTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTACA AAAAGACTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTCCAAGAAG CAATCAAAGC ATTCCATGAC AATGAAAAAA TCAGTGAGGC TCTACCAATT
    GAGAAAGAAG CTGAAGAAAA GTCTCTACCA GTTCTGCAAG AGAAGAACAA AGAAAGCCAG
    GATCTACAGA ATGCATTTTC ACCTCATCCA GTTTCAGAAG AAGCGTTACC AGAAACAGAA
    GATAAAACTA TACTTAGTAC TGATACAAAT AAAACTCCAA AAGCTGAAGA AGTCAAATCA
    AGTGATAGTT TCTCTGAACT GACTGTGCGG GCTCAGCTTG GTGCAAAATC AGAAAAGATG
    CTGAAGAAAG GAAAGAGCAT TTCTGACATG TTGCTAGTTA ATATCTTGGT AAATGCTATT
    AATGAGGTAC CTGTGGATCA AGGCTGGGTC TTGGATGGTT TTCCAATGAC GTTAAACCAA
    GCAAAGCTTC TGGAGGAAGC TCTCACGGGC TACAACAGAG AGGCCCTAGA ACTGGAAGAA
    AAAAAAACAC AAATACCCAC ATTGGCTGTT GATGCTAGCA CCTCCAAAGA AGTGCCTCTT
    CCCCCTTCTG CATTTGACTT TGTCATGTTA TTAGATATTT CAGATAATTC CTTGCTGGAC
    CGCATGAATT ACACAATGGC TGAAGCATTT TCAAGTGAAA CTTCTCATGA AGACATCACT
    CAACGTGTAA CTGCTGAAAA GCAAGATATG AATGAGGACC AGAATTTAAG AGACCAGATT
    CAGCACAGAA TTATAGGCTT CTTGGACAAC TGGCCTTTGT TGGAGCAATG GTTTACAGAG
    CCAGAAAATA TTTTGATAAA TATCAATGCT GAAGTAGATA AAGAGTCTTT ATGCAATAAA
    GTAAAAGAAA TGTTTATGAC TGAAATAATG AAAAAAGAGA ATAAAGTTAA AAAGAAATTA
    GAAGAAAAGG AAGCTGAGAA AAAAGAAGTT CCCCTGGGTG AGCCTCCGCC TCCTCCTCCT
    CCCCCTCCCC CTCCTCCTCC TGAGCCAGAA AAAGAGAAGG AAACCCATCC TCAACGAGAG
    CATTCAAAAA CTCCTACTGC AAAAGGGAAA CCACCAGCAG AACCCTTGAC TGGTAAGCCA
    GACTCTCTTC AGGAAGGAAA AGGAAAGAAA GGGGACACTT CACTCAAAAG AAAAGGTTCT
    CCTAAAGGAA AATCACCAGG AGGGAAAGGA CCACTAAAGA AATCACCTGC TGATTCTACA
    GACATGTCAC CTGTTCCCAT AGTGCCACCA CCACCCAAGC CAGGATCAGA AGAATGGGTC
    TACGTGAATG AACCAATTCC TGAGGAAATG CCTTCATTTT TAGTGCCTTA CTGGGAACTA
    ATCGAAAATT CCTACATAAA CTCCATCAAA ACAGTACTCA GGCATCTGAG AGAAGAACAG
    CATAGTGTGG TTGCTTACTT ATACGAGGTT AGAACAAATT TCCAGCAGTT TCTATGGCGT
    CCAGATCACA AGCAAGATTT TGTATCTCAA TGGCAGGCTG ATTTCAATGC TCTTCCTGAT
    GACCTGTGGG ATGATGAAGA AACAAAGTCT GAACTACACC AAAGAGTAAA TGATTTAAGA
    GACCGCCTGT GGGACATCTG TGATGCCCGG AAGGAAGAAG CAGAGCAGGA GCGTCAGGAT
    ATCATTAACG AGAGCTGGTT GCAAGACTCA ATCGGAATAA CGATGAACCA TTTCTTCTCA
    CTAATGCAGA CAGAACTGAA CCGTTTCCAA GATACAAAGA GACTCCTTCA AGATTATTAC
    AGGGGAATGG AATGCAAAAT TCCAATCGAT GACAGTAAGA GATTTACTCG AATCCCGTTG
    GTCCAGCTGG ATAGTAGTAG AGACATTTTG GAAAGCCAAC TCAGAATTCC TCTAATTCCA
    CGAATATCCA TTTCCCCGGA TTCCGCTATG ACCAAACCAA AAACAAAGGC CATACTAAAG
    GGCAAGATCG ATTACTCGCT GGAAAATGTG GAGTTAAACT TTGAGGCAGA TGAGAAGTTG
    GTGATGGACA CCTGGCAGCA GGCTTCTTTA GCAATATCTC ACATGGTGGC TGCTGAAATT
    CATCAGAGGC TCATGGAAGA AGAAAAGGAA AACCAACCAG CAGACACAAA AGAAAAATCT
    CCTCAGATGG GGACAAATAA AAAAGCCAAA GAGGAACCAC CCAAGAAAGA AAAGGCAGAC
    AAAAAAGCAA AAGGCAGATC CCCGCCGACA GCAGAAGCCA CGCCTGTGAC AGTCACAGCA
    GAGGAGATTA CCCAAAAGGA GAAGAGAAAC GAACTGATGC TCAAAATCAA GGAAGAGCAC
    CTCGCTGCCC TGCAGTTTGA AGAGATAGCC ACACAGTTCC GGCTTGAACT GATAAAGACA
    AAAGCATTGG CTTTCCTTGA AGATTTACTA ACAAAGGTGG TCGATGTATA TAAACTCTTG
    GAAAAGTGGC TTGGTGAGAG GTACTTGAAT GAAATGGCCA GTGTGGAAAA ATTAACTGAG
    GTGGCCCGTT ATCACATTGA AACATCAACA AAAATTCAGA ATGAACTTTA CCTAGACCAA
    GAAGATTTCT TCATTAATGG AGATCTGAAA GTCTTTCCAG ATCCCCCTCC CCCAGTGCGC
    CCCCCACCTG TGGAAAAGGA AGAAAATGGC ACCCTGACCA TCGAACAGCT TGACAGTCTT
    AAAGATCAGT TCTTAGATAT TGCACCCAAA GGCATAATAG GACATAAAGC ATTTACTGAC
    ATTCTGCTGG ATCTGGTAAC CCTGAACCTT GGAACAAACA ACTTACCTAG TAGTTGGATG
    CACCTCACGC AACCAGAATT GCAGGAGTTA ACATCTTTAG TAATAGTAAA TTCGGAGTTT
    GTGGACTGGC GGAAATTCCT ATTAGTAATC TCGATGCCTT GGCCCATCCC CATGGAGGAA
    GAGCTCCTCG AAACCCTGCA GAGGTTCAAG GCGGTGGATG AAGAGCAGTT GGGCACCATC
    ACTTTTGAGC AGTATATGCA GGCTGGTCTG TGGTTTACAG GAGATGAATA TATAAAAATT
    CCAGAAAATC CTCTTGAACC TCTGCCGTTC AATAGGCAGG AGCATCTTAT AGAGTTTTTC
    TTTAGGCTAT TTGCTGACCA TGAGAAGGAT CCACCTCAAC TTGACTACAC ACAGATGCTG
    CTCTATTTTG CTTGCCATCC AGATGCTATG GAAGGGGTCT ACAGGGCCCT CAGTGTGGCC
    ATAGGAACTC ATGTCTTCCA ACCAGTCGAA ACTATTGCTA TTATTGGAGA AAAGTCCTCC
    TCCTTGACTG ATGCAAGTCC AGTTGAGGAG TATCCTGAAC TCGAGGGAAA CTTTATGCAT
    GAGGAAGGAG AATTAAAAGA AGACAGGGAG GAAAAGGAAG TAGAAATCCC TGAGAATGCA
    AACACTGAAA AGGTATCCCT GGAAAGACTA CTCAAAGTGT TCCAAGGAGG AAAAGAAGTA
    CAGGATGCTA ACAGATTCAC CAGCCACCTA CAGATAGAGA ACACTTACTC AGAGAACTTC
    GTAAAAGTAT TTCAAGACCT AGGTGCCAAG GATCTGGAGC CAGTTGAAGT TGCTGTTCTC
    TTGAAGCATC CTTTTATTCA AGACCTGATT TCAAATTATT CAGACTATAA AATCCCTGAT
    ATTAAAATAA TTCTCCAAAG GAGTGAGCAT GTACAAGGAA GTGATGGAGA AAGATCTCCT
    TCAAGACTTA CAGAGGAAAA GAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273633.1
    CDS643..5589
    Translation

    Target ORF information:

    RefSeq Version XM_022417925.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris sperm flagellar protein 2 (LOC489235), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417925.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCAAACTA TGCAACCCCT GGCAAACGTC AGATACCAAA GTATGAAAAG TGAGGCTTTT 
    CGAGATCAAC GCCTGAGCAG GAGACGACAA AATGAAATAA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC TGCATCAAAT CGTACTTTGA GAGCACTGGA GGCCCAAAAA
    ATGATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AGATTAAGAA GTTTGAAGCT
    TTAATAAAAA AGGATCTCCA GGCCAAAGAA AGTGCCTCCA AGACTTCTTT AGATACAGCA
    GGCCAGACAA CTACTGATTT GTTAAACACT TATTCGGATG ATGACTACAT TAAAAAAATC
    CAAAAGCGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTATTAATGG ACCAGTTAAT AGCCCACGAA GCACAAGAGG AGGCGTATCG GGAGGAGCAG
    CTGATTAACC GGCTGATGCG GCAGTCCCAG CAGGAGCGCA GGATCTCTGT GCAGCTCATG
    CATGTTCGGC ATGAAAAGGA AGTTTTGTGG CAAAACAGAA TTTTCCGAGA AAAACAGTAT
    GAGGAAAGAC GACTTAAAGA TTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTGGCAAAA
    CAAGCCAAGA TTGATTTTCA GGAACAAGCC ATTAGAGAGA AGGAAATTCA TGAACAGATC
    ACTGTGGAAA GAGCTCAAGC TCGTTACAAA AAGCATTATA CAATATGTGC AGAAATTTTG
    GATCAAATAC TTGATCTTTC CACTAAAGTG TCAGACTACC GGATGTTGAC AAATAATTTG
    ATTCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TTAATGGAAA GCCCATATAC
    AAGCAAGCCT CTATTAAGCA ACTACCAGCC ACGCCCTCTG CAGAACAAGT TATAGAACTG
    GAGAAGAGGG ACTTGCTGGA TAGCAATGAT TATGAAGAAT ATAAGAATAT GGTTGGAGAG
    TGGGCTTTAC CAGAAGAAAT GGTTGATAAT TTACCACCCT CCAACAATAC CATATTGGGT
    CATGTGATTC ACAGATTAAT TGAAAAATCT CATCCTCCTC AAGTCAAATC AACAGTACTA
    GAATTACCTT CATTTGCTAT TAAAGGGTGC TTAGTTGGAA AAACATTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTACA AAAAGACTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTCCAAGAAG CAATCAAAGC ATTCCATGAC AATGAAAAAA TCAGTGAGGC TCTACCAATT
    GAGAAAGAAG CTGAAGAAAA GTCTCTACCA GTTCTGCAAG AGAAGAACAA AGAAAGCCAG
    GATCTACAGA ATGCATTTTC ACCTCATCCA GTTTCAGAAG AAGCGTTACC AGAAACAGAA
    GATAAAACTA TACTTAGTAC TGATACAAAT AAAACTCCAA AAGCTGAAGA AGTCAAATCA
    AGTGATAGTT TCTCTGAACT GACTGTGCGG GCTCAGCTTG GTGCAAAATC AGAAAAGATG
    CTGAAGAAAG GAAAGAGCAT TTCTGACATG TTGCTAGTTA ATATCTTGGT AAATGCTATT
    AATGAGGTAC CTGTGGATCA AGGCTGGGTC TTGGATGGTT TTCCAATGAC GTTAAACCAA
    GCAAAGCTTC TGGAGGAAGC TCTCACGGGC TACAACAGAG AGGCCCTAGA ACTGGAAGAA
    AAAAAAACAC AAATACCCAC ATTGGCTGTT GATGCTAGCA CCTCCAAAGA AGTGCCTCTT
    CCCCCTTCTG CATTTGACTT TGTCATGTTA TTAGATATTT CAGATAATTC CTTGCTGGAC
    CGCATGAATT ACACAATGGC TGAAGCATTT TCAAGTGAAA CTTCTCATGA AGACATCACT
    CAACGTGTAA CTGCTGAAAA GCAAGATATG AATGAGGACC AGAATTTAAG AGACCAGATT
    CAGCACAGAA TTATAGGCTT CTTGGACAAC TGGCCTTTGT TGGAGCAATG GTTTACAGAG
    CCAGAAAATA TTTTGATAAA TATCAATGCT GAAGTAGATA AAGAGTCTTT ATGCAATAAA
    GTAAAAGAAA TGTTTATGAC TGAAATAATG AAAAAAGAGA ATAAAGTTAA AAAGAAATTA
    GAAGAAAAGG AAGCTGAGAA AAAAGAAGTT CCCCTGGGTG AGCCTCCGCC TCCTCCTCCT
    CCCCCTCCCC CTCCTCCTCC TGAGCCAGAA AAAGAGAAGG AAACCCATCC TCAACGAGAG
    CATTCAAAAA CTCCTACTGC AAAAGGGAAA CCACCAGCAG AACCCTTGAC TGGTAAGCCA
    GACTCTCTTC AGGAAGGAAA AGGAAAGAAA GGGGACACTT CACTCAAAAG AAAAGGTTCT
    CCTAAAGGAA AATCACCAGG AGGGAAAGGA CCACTAAAGA AATCACCTGC TGATTCTACA
    GACATGTCAC CTGTTCCCAT AGTGCCACCA CCACCCAAGC CAGGATCAGA AGAATGGGTC
    TACGTGAATG AACCAATTCC TGAGGAAATG CCTTCATTTT TAGTGCCTTA CTGGGAACTA
    ATCGAAAATT CCTACATAAA CTCCATCAAA ACAGTACTCA GGCATCTGAG AGAAGAACAG
    CATAGTGTGG TTGCTTACTT ATACGAGGTT AGAACAAATT TCCAGCAGTT TCTATGGCGT
    CCAGATCACA AGCAAGATTT TGTATCTCAA TGGCAGGCTG ATTTCAATGC TCTTCCTGAT
    GACCTGTGGG ATGATGAAGA AACAAAGTCT GAACTACACC AAAGAGTAAA TGATTTAAGA
    GACCGCCTGT GGGACATCTG TGATGCCCGG AAGGAAGAAG CAGAGCAGGA GCGTCAGGAT
    ATCATTAACG AGAGCTGGTT GCAAGACTCA ATCGGAATAA CGATGAACCA TTTCTTCTCA
    CTAATGCAGA CAGAACTGAA CCGTTTCCAA GATACAAAGA GACTCCTTCA AGATTATTAC
    AGGGGAATGG AATGCAAAAT TCCAATCGAT GACAGTAAGA GATTTACTCG AATCCCGTTG
    GTCCAGCTGG ATAGTAGTAG AGACATTTTG GAAAGCCAAC TCAGAATTCC TCTAATTCCA
    CGAATATCCA TTTCCCCGGA TTCCGCTATG ACCAAACCAA AAACAAAGGC CATACTAAAG
    GGCAAGATCG ATTACTCGCT GGAAAATGTG GAGTTAAACT TTGAGGCAGA TGAGAAGTTG
    GTGATGGACA CCTGGCAGCA GGCTTCTTTA GCAATATCTC ACATGGTGGC TGCTGAAATT
    CATCAGAGGC TCATGGAAGA AGAAAAGGAA AACCAACCAG CAGACACAAA AGAAAAATCT
    CCTCAGATGG GGACAAATAA AAAAGCCAAA GAGGAACCAC CCAAGAAAGA AAAGGCAGAC
    AAAAAAGCAA AAGGCAGATC CCCGCCGACA GCAGAAGCCA CGCCTGTGAC AGTCACAGCA
    GAGGAGATTA CCCAAAAGGA GAAGAGAAAC GAACTGATGC TCAAAATCAA GGAAGAGCAC
    CTCGCTGCCC TGCAGTTTGA AGAGATAGCC ACACAGTTCC GGCTTGAACT GATAAAGACA
    AAAGCATTGG CTTTCCTTGA AGATTTACTA ACAAAGGTGG TCGATGTATA TAAACTCTTG
    GAAAAGTGGC TTGGTGAGAG GTACTTGAAT GAAATGGCCA GTGTGGAAAA ATTAACTGAG
    GTGGCCCGTT ATCACATTGA AACATCAACA AAAATTCAGA ATGAACTTTA CCTAGACCAA
    GAAGATTTCT TCATTAATGG AGATCTGAAA GTCTTTCCAG ATCCCCCTCC CCCAGTGCGC
    CCCCCACCTG TGGAAAAGGA AGAAAATGGC ACCCTGACCA TCGAACAGCT TGACAGTCTT
    AAAGATCAGT TCTTAGATAT TGCACCCAAA GGCATAATAG GACATAAAGC ATTTACTGAC
    ATTCTGCTGG ATCTGGTAAC CCTGAACCTT GGAACAAACA ACTTACCTAG TAGTTGGATG
    CACCTCACGC AACCAGAATT GCAGGAGTTA ACATCTTTAG TAATAGTAAA TTCGGAGTTT
    GTGGACTGGC GGAAATTCCT ATTAGTAATC TCGATGCCTT GGCCCATCCC CATGGAGGAA
    GAGCTCCTCG AAACCCTGCA GAGGTTCAAG GCGGTGGATG AAGAGCAGTT GGGCACCATC
    ACTTTTGAGC AGTATATGCA GGCTGGTCTG TGGTTTACAG GAGATGAATA TATAAAAATT
    CCAGAAAATC CTCTTGAACC TCTGCCGTTC AATAGGCAGG AGCATCTTAT AGAGTTTTTC
    TTTAGGCTAT TTGCTGACCA TGAGAAGGAT CCACCTCAAC TTGACTACAC ACAGATGCTG
    CTCTATTTTG CTTGCCATCC AGATGCTATG GAAGGGGTCT ACAGGGCCCT CAGTGTGGCC
    ATAGGAACTC ATGTCTTCCA ACCAGTCGAA ACTATTGCTA TTATTGGAGA AAAGTCCTCC
    TCCTTGACTG ATGCAAGTCC AGTTGAGGAG TATCCTGAAC TCGAGGGAAA CTTTATGCAT
    GAGGAAGGAG AATTAAAAGA AGACAGGGAG GAAAAGGAAG TAGAAATCCC TGAGAATGCA
    AACACTGAAA AGGTATCCCT GGAAAGACTA CTCAAAGTGT TCCAAGGAGG AAAAGAAGTA
    CAGGATGCTA ACAGATTCAC CAGCCACCTA CAGATAGAGA ACACTTACTC AGAGAACTTC
    GTAAAAGTAT TTCAAGACCT AGGTGCCAAG GATCTGGAGC CAGTTGAAGT TGCTGTTCTC
    TTGAAGCATC CTTTTATTCA AGACCTGATT TCAAATTATT CAGACTATAA AATCCCTGAT
    ATTAAAATAA TTCTCCAAAG GAGTGAGCAT GTACAAGGAA GTGATGGAGA AAGATCTCCT
    TCAAGACTTA CAGAGGAAAA GAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287739
    Clone ID Related Accession (Same CDS sequence) XM_022417925.1 , XM_022417927.1 , XM_022417928.1 , XM_022417926.1
    Accession Version XM_022417927.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product sperm flagellar protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCAAACTA TGCAACCCCT GGCAAACGTC AGATACCAAA GTATGAAAAG TGAGGCTTTT 
    CGAGATCAAC GCCTGAGCAG GAGACGACAA AATGAAATAA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC TGCATCAAAT CGTACTTTGA GAGCACTGGA GGCCCAAAAA
    ATGATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AGATTAAGAA GTTTGAAGCT
    TTAATAAAAA AGGATCTCCA GGCCAAAGAA AGTGCCTCCA AGACTTCTTT AGATACAGCA
    GGCCAGACAA CTACTGATTT GTTAAACACT TATTCGGATG ATGACTACAT TAAAAAAATC
    CAAAAGCGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTATTAATGG ACCAGTTAAT AGCCCACGAA GCACAAGAGG AGGCGTATCG GGAGGAGCAG
    CTGATTAACC GGCTGATGCG GCAGTCCCAG CAGGAGCGCA GGATCTCTGT GCAGCTCATG
    CATGTTCGGC ATGAAAAGGA AGTTTTGTGG CAAAACAGAA TTTTCCGAGA AAAACAGTAT
    GAGGAAAGAC GACTTAAAGA TTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTGGCAAAA
    CAAGCCAAGA TTGATTTTCA GGAACAAGCC ATTAGAGAGA AGGAAATTCA TGAACAGATC
    ACTGTGGAAA GAGCTCAAGC TCGTTACAAA AAGCATTATA CAATATGTGC AGAAATTTTG
    GATCAAATAC TTGATCTTTC CACTAAAGTG TCAGACTACC GGATGTTGAC AAATAATTTG
    ATTCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TTAATGGAAA GCCCATATAC
    AAGCAAGCCT CTATTAAGCA ACTACCAGCC ACGCCCTCTG CAGAACAAGT TATAGAACTG
    GAGAAGAGGG ACTTGCTGGA TAGCAATGAT TATGAAGAAT ATAAGAATAT GGTTGGAGAG
    TGGGCTTTAC CAGAAGAAAT GGTTGATAAT TTACCACCCT CCAACAATAC CATATTGGGT
    CATGTGATTC ACAGATTAAT TGAAAAATCT CATCCTCCTC AAGTCAAATC AACAGTACTA
    GAATTACCTT CATTTGCTAT TAAAGGGTGC TTAGTTGGAA AAACATTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTACA AAAAGACTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTCCAAGAAG CAATCAAAGC ATTCCATGAC AATGAAAAAA TCAGTGAGGC TCTACCAATT
    GAGAAAGAAG CTGAAGAAAA GTCTCTACCA GTTCTGCAAG AGAAGAACAA AGAAAGCCAG
    GATCTACAGA ATGCATTTTC ACCTCATCCA GTTTCAGAAG AAGCGTTACC AGAAACAGAA
    GATAAAACTA TACTTAGTAC TGATACAAAT AAAACTCCAA AAGCTGAAGA AGTCAAATCA
    AGTGATAGTT TCTCTGAACT GACTGTGCGG GCTCAGCTTG GTGCAAAATC AGAAAAGATG
    CTGAAGAAAG GAAAGAGCAT TTCTGACATG TTGCTAGTTA ATATCTTGGT AAATGCTATT
    AATGAGGTAC CTGTGGATCA AGGCTGGGTC TTGGATGGTT TTCCAATGAC GTTAAACCAA
    GCAAAGCTTC TGGAGGAAGC TCTCACGGGC TACAACAGAG AGGCCCTAGA ACTGGAAGAA
    AAAAAAACAC AAATACCCAC ATTGGCTGTT GATGCTAGCA CCTCCAAAGA AGTGCCTCTT
    CCCCCTTCTG CATTTGACTT TGTCATGTTA TTAGATATTT CAGATAATTC CTTGCTGGAC
    CGCATGAATT ACACAATGGC TGAAGCATTT TCAAGTGAAA CTTCTCATGA AGACATCACT
    CAACGTGTAA CTGCTGAAAA GCAAGATATG AATGAGGACC AGAATTTAAG AGACCAGATT
    CAGCACAGAA TTATAGGCTT CTTGGACAAC TGGCCTTTGT TGGAGCAATG GTTTACAGAG
    CCAGAAAATA TTTTGATAAA TATCAATGCT GAAGTAGATA AAGAGTCTTT ATGCAATAAA
    GTAAAAGAAA TGTTTATGAC TGAAATAATG AAAAAAGAGA ATAAAGTTAA AAAGAAATTA
    GAAGAAAAGG AAGCTGAGAA AAAAGAAGTT CCCCTGGGTG AGCCTCCGCC TCCTCCTCCT
    CCCCCTCCCC CTCCTCCTCC TGAGCCAGAA AAAGAGAAGG AAACCCATCC TCAACGAGAG
    CATTCAAAAA CTCCTACTGC AAAAGGGAAA CCACCAGCAG AACCCTTGAC TGGTAAGCCA
    GACTCTCTTC AGGAAGGAAA AGGAAAGAAA GGGGACACTT CACTCAAAAG AAAAGGTTCT
    CCTAAAGGAA AATCACCAGG AGGGAAAGGA CCACTAAAGA AATCACCTGC TGATTCTACA
    GACATGTCAC CTGTTCCCAT AGTGCCACCA CCACCCAAGC CAGGATCAGA AGAATGGGTC
    TACGTGAATG AACCAATTCC TGAGGAAATG CCTTCATTTT TAGTGCCTTA CTGGGAACTA
    ATCGAAAATT CCTACATAAA CTCCATCAAA ACAGTACTCA GGCATCTGAG AGAAGAACAG
    CATAGTGTGG TTGCTTACTT ATACGAGGTT AGAACAAATT TCCAGCAGTT TCTATGGCGT
    CCAGATCACA AGCAAGATTT TGTATCTCAA TGGCAGGCTG ATTTCAATGC TCTTCCTGAT
    GACCTGTGGG ATGATGAAGA AACAAAGTCT GAACTACACC AAAGAGTAAA TGATTTAAGA
    GACCGCCTGT GGGACATCTG TGATGCCCGG AAGGAAGAAG CAGAGCAGGA GCGTCAGGAT
    ATCATTAACG AGAGCTGGTT GCAAGACTCA ATCGGAATAA CGATGAACCA TTTCTTCTCA
    CTAATGCAGA CAGAACTGAA CCGTTTCCAA GATACAAAGA GACTCCTTCA AGATTATTAC
    AGGGGAATGG AATGCAAAAT TCCAATCGAT GACAGTAAGA GATTTACTCG AATCCCGTTG
    GTCCAGCTGG ATAGTAGTAG AGACATTTTG GAAAGCCAAC TCAGAATTCC TCTAATTCCA
    CGAATATCCA TTTCCCCGGA TTCCGCTATG ACCAAACCAA AAACAAAGGC CATACTAAAG
    GGCAAGATCG ATTACTCGCT GGAAAATGTG GAGTTAAACT TTGAGGCAGA TGAGAAGTTG
    GTGATGGACA CCTGGCAGCA GGCTTCTTTA GCAATATCTC ACATGGTGGC TGCTGAAATT
    CATCAGAGGC TCATGGAAGA AGAAAAGGAA AACCAACCAG CAGACACAAA AGAAAAATCT
    CCTCAGATGG GGACAAATAA AAAAGCCAAA GAGGAACCAC CCAAGAAAGA AAAGGCAGAC
    AAAAAAGCAA AAGGCAGATC CCCGCCGACA GCAGAAGCCA CGCCTGTGAC AGTCACAGCA
    GAGGAGATTA CCCAAAAGGA GAAGAGAAAC GAACTGATGC TCAAAATCAA GGAAGAGCAC
    CTCGCTGCCC TGCAGTTTGA AGAGATAGCC ACACAGTTCC GGCTTGAACT GATAAAGACA
    AAAGCATTGG CTTTCCTTGA AGATTTACTA ACAAAGGTGG TCGATGTATA TAAACTCTTG
    GAAAAGTGGC TTGGTGAGAG GTACTTGAAT GAAATGGCCA GTGTGGAAAA ATTAACTGAG
    GTGGCCCGTT ATCACATTGA AACATCAACA AAAATTCAGA ATGAACTTTA CCTAGACCAA
    GAAGATTTCT TCATTAATGG AGATCTGAAA GTCTTTCCAG ATCCCCCTCC CCCAGTGCGC
    CCCCCACCTG TGGAAAAGGA AGAAAATGGC ACCCTGACCA TCGAACAGCT TGACAGTCTT
    AAAGATCAGT TCTTAGATAT TGCACCCAAA GGCATAATAG GACATAAAGC ATTTACTGAC
    ATTCTGCTGG ATCTGGTAAC CCTGAACCTT GGAACAAACA ACTTACCTAG TAGTTGGATG
    CACCTCACGC AACCAGAATT GCAGGAGTTA ACATCTTTAG TAATAGTAAA TTCGGAGTTT
    GTGGACTGGC GGAAATTCCT ATTAGTAATC TCGATGCCTT GGCCCATCCC CATGGAGGAA
    GAGCTCCTCG AAACCCTGCA GAGGTTCAAG GCGGTGGATG AAGAGCAGTT GGGCACCATC
    ACTTTTGAGC AGTATATGCA GGCTGGTCTG TGGTTTACAG GAGATGAATA TATAAAAATT
    CCAGAAAATC CTCTTGAACC TCTGCCGTTC AATAGGCAGG AGCATCTTAT AGAGTTTTTC
    TTTAGGCTAT TTGCTGACCA TGAGAAGGAT CCACCTCAAC TTGACTACAC ACAGATGCTG
    CTCTATTTTG CTTGCCATCC AGATGCTATG GAAGGGGTCT ACAGGGCCCT CAGTGTGGCC
    ATAGGAACTC ATGTCTTCCA ACCAGTCGAA ACTATTGCTA TTATTGGAGA AAAGTCCTCC
    TCCTTGACTG ATGCAAGTCC AGTTGAGGAG TATCCTGAAC TCGAGGGAAA CTTTATGCAT
    GAGGAAGGAG AATTAAAAGA AGACAGGGAG GAAAAGGAAG TAGAAATCCC TGAGAATGCA
    AACACTGAAA AGGTATCCCT GGAAAGACTA CTCAAAGTGT TCCAAGGAGG AAAAGAAGTA
    CAGGATGCTA ACAGATTCAC CAGCCACCTA CAGATAGAGA ACACTTACTC AGAGAACTTC
    GTAAAAGTAT TTCAAGACCT AGGTGCCAAG GATCTGGAGC CAGTTGAAGT TGCTGTTCTC
    TTGAAGCATC CTTTTATTCA AGACCTGATT TCAAATTATT CAGACTATAA AATCCCTGAT
    ATTAAAATAA TTCTCCAAAG GAGTGAGCAT GTACAAGGAA GTGATGGAGA AAGATCTCCT
    TCAAGACTTA CAGAGGAAAA GAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273635.1
    CDS371..5317
    Translation

    Target ORF information:

    RefSeq Version XM_022417927.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris sperm flagellar protein 2 (LOC489235), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417927.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCAAACTA TGCAACCCCT GGCAAACGTC AGATACCAAA GTATGAAAAG TGAGGCTTTT 
    CGAGATCAAC GCCTGAGCAG GAGACGACAA AATGAAATAA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC TGCATCAAAT CGTACTTTGA GAGCACTGGA GGCCCAAAAA
    ATGATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AGATTAAGAA GTTTGAAGCT
    TTAATAAAAA AGGATCTCCA GGCCAAAGAA AGTGCCTCCA AGACTTCTTT AGATACAGCA
    GGCCAGACAA CTACTGATTT GTTAAACACT TATTCGGATG ATGACTACAT TAAAAAAATC
    CAAAAGCGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTATTAATGG ACCAGTTAAT AGCCCACGAA GCACAAGAGG AGGCGTATCG GGAGGAGCAG
    CTGATTAACC GGCTGATGCG GCAGTCCCAG CAGGAGCGCA GGATCTCTGT GCAGCTCATG
    CATGTTCGGC ATGAAAAGGA AGTTTTGTGG CAAAACAGAA TTTTCCGAGA AAAACAGTAT
    GAGGAAAGAC GACTTAAAGA TTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTGGCAAAA
    CAAGCCAAGA TTGATTTTCA GGAACAAGCC ATTAGAGAGA AGGAAATTCA TGAACAGATC
    ACTGTGGAAA GAGCTCAAGC TCGTTACAAA AAGCATTATA CAATATGTGC AGAAATTTTG
    GATCAAATAC TTGATCTTTC CACTAAAGTG TCAGACTACC GGATGTTGAC AAATAATTTG
    ATTCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TTAATGGAAA GCCCATATAC
    AAGCAAGCCT CTATTAAGCA ACTACCAGCC ACGCCCTCTG CAGAACAAGT TATAGAACTG
    GAGAAGAGGG ACTTGCTGGA TAGCAATGAT TATGAAGAAT ATAAGAATAT GGTTGGAGAG
    TGGGCTTTAC CAGAAGAAAT GGTTGATAAT TTACCACCCT CCAACAATAC CATATTGGGT
    CATGTGATTC ACAGATTAAT TGAAAAATCT CATCCTCCTC AAGTCAAATC AACAGTACTA
    GAATTACCTT CATTTGCTAT TAAAGGGTGC TTAGTTGGAA AAACATTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTACA AAAAGACTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTCCAAGAAG CAATCAAAGC ATTCCATGAC AATGAAAAAA TCAGTGAGGC TCTACCAATT
    GAGAAAGAAG CTGAAGAAAA GTCTCTACCA GTTCTGCAAG AGAAGAACAA AGAAAGCCAG
    GATCTACAGA ATGCATTTTC ACCTCATCCA GTTTCAGAAG AAGCGTTACC AGAAACAGAA
    GATAAAACTA TACTTAGTAC TGATACAAAT AAAACTCCAA AAGCTGAAGA AGTCAAATCA
    AGTGATAGTT TCTCTGAACT GACTGTGCGG GCTCAGCTTG GTGCAAAATC AGAAAAGATG
    CTGAAGAAAG GAAAGAGCAT TTCTGACATG TTGCTAGTTA ATATCTTGGT AAATGCTATT
    AATGAGGTAC CTGTGGATCA AGGCTGGGTC TTGGATGGTT TTCCAATGAC GTTAAACCAA
    GCAAAGCTTC TGGAGGAAGC TCTCACGGGC TACAACAGAG AGGCCCTAGA ACTGGAAGAA
    AAAAAAACAC AAATACCCAC ATTGGCTGTT GATGCTAGCA CCTCCAAAGA AGTGCCTCTT
    CCCCCTTCTG CATTTGACTT TGTCATGTTA TTAGATATTT CAGATAATTC CTTGCTGGAC
    CGCATGAATT ACACAATGGC TGAAGCATTT TCAAGTGAAA CTTCTCATGA AGACATCACT
    CAACGTGTAA CTGCTGAAAA GCAAGATATG AATGAGGACC AGAATTTAAG AGACCAGATT
    CAGCACAGAA TTATAGGCTT CTTGGACAAC TGGCCTTTGT TGGAGCAATG GTTTACAGAG
    CCAGAAAATA TTTTGATAAA TATCAATGCT GAAGTAGATA AAGAGTCTTT ATGCAATAAA
    GTAAAAGAAA TGTTTATGAC TGAAATAATG AAAAAAGAGA ATAAAGTTAA AAAGAAATTA
    GAAGAAAAGG AAGCTGAGAA AAAAGAAGTT CCCCTGGGTG AGCCTCCGCC TCCTCCTCCT
    CCCCCTCCCC CTCCTCCTCC TGAGCCAGAA AAAGAGAAGG AAACCCATCC TCAACGAGAG
    CATTCAAAAA CTCCTACTGC AAAAGGGAAA CCACCAGCAG AACCCTTGAC TGGTAAGCCA
    GACTCTCTTC AGGAAGGAAA AGGAAAGAAA GGGGACACTT CACTCAAAAG AAAAGGTTCT
    CCTAAAGGAA AATCACCAGG AGGGAAAGGA CCACTAAAGA AATCACCTGC TGATTCTACA
    GACATGTCAC CTGTTCCCAT AGTGCCACCA CCACCCAAGC CAGGATCAGA AGAATGGGTC
    TACGTGAATG AACCAATTCC TGAGGAAATG CCTTCATTTT TAGTGCCTTA CTGGGAACTA
    ATCGAAAATT CCTACATAAA CTCCATCAAA ACAGTACTCA GGCATCTGAG AGAAGAACAG
    CATAGTGTGG TTGCTTACTT ATACGAGGTT AGAACAAATT TCCAGCAGTT TCTATGGCGT
    CCAGATCACA AGCAAGATTT TGTATCTCAA TGGCAGGCTG ATTTCAATGC TCTTCCTGAT
    GACCTGTGGG ATGATGAAGA AACAAAGTCT GAACTACACC AAAGAGTAAA TGATTTAAGA
    GACCGCCTGT GGGACATCTG TGATGCCCGG AAGGAAGAAG CAGAGCAGGA GCGTCAGGAT
    ATCATTAACG AGAGCTGGTT GCAAGACTCA ATCGGAATAA CGATGAACCA TTTCTTCTCA
    CTAATGCAGA CAGAACTGAA CCGTTTCCAA GATACAAAGA GACTCCTTCA AGATTATTAC
    AGGGGAATGG AATGCAAAAT TCCAATCGAT GACAGTAAGA GATTTACTCG AATCCCGTTG
    GTCCAGCTGG ATAGTAGTAG AGACATTTTG GAAAGCCAAC TCAGAATTCC TCTAATTCCA
    CGAATATCCA TTTCCCCGGA TTCCGCTATG ACCAAACCAA AAACAAAGGC CATACTAAAG
    GGCAAGATCG ATTACTCGCT GGAAAATGTG GAGTTAAACT TTGAGGCAGA TGAGAAGTTG
    GTGATGGACA CCTGGCAGCA GGCTTCTTTA GCAATATCTC ACATGGTGGC TGCTGAAATT
    CATCAGAGGC TCATGGAAGA AGAAAAGGAA AACCAACCAG CAGACACAAA AGAAAAATCT
    CCTCAGATGG GGACAAATAA AAAAGCCAAA GAGGAACCAC CCAAGAAAGA AAAGGCAGAC
    AAAAAAGCAA AAGGCAGATC CCCGCCGACA GCAGAAGCCA CGCCTGTGAC AGTCACAGCA
    GAGGAGATTA CCCAAAAGGA GAAGAGAAAC GAACTGATGC TCAAAATCAA GGAAGAGCAC
    CTCGCTGCCC TGCAGTTTGA AGAGATAGCC ACACAGTTCC GGCTTGAACT GATAAAGACA
    AAAGCATTGG CTTTCCTTGA AGATTTACTA ACAAAGGTGG TCGATGTATA TAAACTCTTG
    GAAAAGTGGC TTGGTGAGAG GTACTTGAAT GAAATGGCCA GTGTGGAAAA ATTAACTGAG
    GTGGCCCGTT ATCACATTGA AACATCAACA AAAATTCAGA ATGAACTTTA CCTAGACCAA
    GAAGATTTCT TCATTAATGG AGATCTGAAA GTCTTTCCAG ATCCCCCTCC CCCAGTGCGC
    CCCCCACCTG TGGAAAAGGA AGAAAATGGC ACCCTGACCA TCGAACAGCT TGACAGTCTT
    AAAGATCAGT TCTTAGATAT TGCACCCAAA GGCATAATAG GACATAAAGC ATTTACTGAC
    ATTCTGCTGG ATCTGGTAAC CCTGAACCTT GGAACAAACA ACTTACCTAG TAGTTGGATG
    CACCTCACGC AACCAGAATT GCAGGAGTTA ACATCTTTAG TAATAGTAAA TTCGGAGTTT
    GTGGACTGGC GGAAATTCCT ATTAGTAATC TCGATGCCTT GGCCCATCCC CATGGAGGAA
    GAGCTCCTCG AAACCCTGCA GAGGTTCAAG GCGGTGGATG AAGAGCAGTT GGGCACCATC
    ACTTTTGAGC AGTATATGCA GGCTGGTCTG TGGTTTACAG GAGATGAATA TATAAAAATT
    CCAGAAAATC CTCTTGAACC TCTGCCGTTC AATAGGCAGG AGCATCTTAT AGAGTTTTTC
    TTTAGGCTAT TTGCTGACCA TGAGAAGGAT CCACCTCAAC TTGACTACAC ACAGATGCTG
    CTCTATTTTG CTTGCCATCC AGATGCTATG GAAGGGGTCT ACAGGGCCCT CAGTGTGGCC
    ATAGGAACTC ATGTCTTCCA ACCAGTCGAA ACTATTGCTA TTATTGGAGA AAAGTCCTCC
    TCCTTGACTG ATGCAAGTCC AGTTGAGGAG TATCCTGAAC TCGAGGGAAA CTTTATGCAT
    GAGGAAGGAG AATTAAAAGA AGACAGGGAG GAAAAGGAAG TAGAAATCCC TGAGAATGCA
    AACACTGAAA AGGTATCCCT GGAAAGACTA CTCAAAGTGT TCCAAGGAGG AAAAGAAGTA
    CAGGATGCTA ACAGATTCAC CAGCCACCTA CAGATAGAGA ACACTTACTC AGAGAACTTC
    GTAAAAGTAT TTCAAGACCT AGGTGCCAAG GATCTGGAGC CAGTTGAAGT TGCTGTTCTC
    TTGAAGCATC CTTTTATTCA AGACCTGATT TCAAATTATT CAGACTATAA AATCCCTGAT
    ATTAAAATAA TTCTCCAAAG GAGTGAGCAT GTACAAGGAA GTGATGGAGA AAGATCTCCT
    TCAAGACTTA CAGAGGAAAA GAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287739
    Clone ID Related Accession (Same CDS sequence) XM_022417925.1 , XM_022417927.1 , XM_022417928.1 , XM_022417926.1
    Accession Version XM_022417928.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product sperm flagellar protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCAAACTA TGCAACCCCT GGCAAACGTC AGATACCAAA GTATGAAAAG TGAGGCTTTT 
    CGAGATCAAC GCCTGAGCAG GAGACGACAA AATGAAATAA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC TGCATCAAAT CGTACTTTGA GAGCACTGGA GGCCCAAAAA
    ATGATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AGATTAAGAA GTTTGAAGCT
    TTAATAAAAA AGGATCTCCA GGCCAAAGAA AGTGCCTCCA AGACTTCTTT AGATACAGCA
    GGCCAGACAA CTACTGATTT GTTAAACACT TATTCGGATG ATGACTACAT TAAAAAAATC
    CAAAAGCGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTATTAATGG ACCAGTTAAT AGCCCACGAA GCACAAGAGG AGGCGTATCG GGAGGAGCAG
    CTGATTAACC GGCTGATGCG GCAGTCCCAG CAGGAGCGCA GGATCTCTGT GCAGCTCATG
    CATGTTCGGC ATGAAAAGGA AGTTTTGTGG CAAAACAGAA TTTTCCGAGA AAAACAGTAT
    GAGGAAAGAC GACTTAAAGA TTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTGGCAAAA
    CAAGCCAAGA TTGATTTTCA GGAACAAGCC ATTAGAGAGA AGGAAATTCA TGAACAGATC
    ACTGTGGAAA GAGCTCAAGC TCGTTACAAA AAGCATTATA CAATATGTGC AGAAATTTTG
    GATCAAATAC TTGATCTTTC CACTAAAGTG TCAGACTACC GGATGTTGAC AAATAATTTG
    ATTCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TTAATGGAAA GCCCATATAC
    AAGCAAGCCT CTATTAAGCA ACTACCAGCC ACGCCCTCTG CAGAACAAGT TATAGAACTG
    GAGAAGAGGG ACTTGCTGGA TAGCAATGAT TATGAAGAAT ATAAGAATAT GGTTGGAGAG
    TGGGCTTTAC CAGAAGAAAT GGTTGATAAT TTACCACCCT CCAACAATAC CATATTGGGT
    CATGTGATTC ACAGATTAAT TGAAAAATCT CATCCTCCTC AAGTCAAATC AACAGTACTA
    GAATTACCTT CATTTGCTAT TAAAGGGTGC TTAGTTGGAA AAACATTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTACA AAAAGACTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTCCAAGAAG CAATCAAAGC ATTCCATGAC AATGAAAAAA TCAGTGAGGC TCTACCAATT
    GAGAAAGAAG CTGAAGAAAA GTCTCTACCA GTTCTGCAAG AGAAGAACAA AGAAAGCCAG
    GATCTACAGA ATGCATTTTC ACCTCATCCA GTTTCAGAAG AAGCGTTACC AGAAACAGAA
    GATAAAACTA TACTTAGTAC TGATACAAAT AAAACTCCAA AAGCTGAAGA AGTCAAATCA
    AGTGATAGTT TCTCTGAACT GACTGTGCGG GCTCAGCTTG GTGCAAAATC AGAAAAGATG
    CTGAAGAAAG GAAAGAGCAT TTCTGACATG TTGCTAGTTA ATATCTTGGT AAATGCTATT
    AATGAGGTAC CTGTGGATCA AGGCTGGGTC TTGGATGGTT TTCCAATGAC GTTAAACCAA
    GCAAAGCTTC TGGAGGAAGC TCTCACGGGC TACAACAGAG AGGCCCTAGA ACTGGAAGAA
    AAAAAAACAC AAATACCCAC ATTGGCTGTT GATGCTAGCA CCTCCAAAGA AGTGCCTCTT
    CCCCCTTCTG CATTTGACTT TGTCATGTTA TTAGATATTT CAGATAATTC CTTGCTGGAC
    CGCATGAATT ACACAATGGC TGAAGCATTT TCAAGTGAAA CTTCTCATGA AGACATCACT
    CAACGTGTAA CTGCTGAAAA GCAAGATATG AATGAGGACC AGAATTTAAG AGACCAGATT
    CAGCACAGAA TTATAGGCTT CTTGGACAAC TGGCCTTTGT TGGAGCAATG GTTTACAGAG
    CCAGAAAATA TTTTGATAAA TATCAATGCT GAAGTAGATA AAGAGTCTTT ATGCAATAAA
    GTAAAAGAAA TGTTTATGAC TGAAATAATG AAAAAAGAGA ATAAAGTTAA AAAGAAATTA
    GAAGAAAAGG AAGCTGAGAA AAAAGAAGTT CCCCTGGGTG AGCCTCCGCC TCCTCCTCCT
    CCCCCTCCCC CTCCTCCTCC TGAGCCAGAA AAAGAGAAGG AAACCCATCC TCAACGAGAG
    CATTCAAAAA CTCCTACTGC AAAAGGGAAA CCACCAGCAG AACCCTTGAC TGGTAAGCCA
    GACTCTCTTC AGGAAGGAAA AGGAAAGAAA GGGGACACTT CACTCAAAAG AAAAGGTTCT
    CCTAAAGGAA AATCACCAGG AGGGAAAGGA CCACTAAAGA AATCACCTGC TGATTCTACA
    GACATGTCAC CTGTTCCCAT AGTGCCACCA CCACCCAAGC CAGGATCAGA AGAATGGGTC
    TACGTGAATG AACCAATTCC TGAGGAAATG CCTTCATTTT TAGTGCCTTA CTGGGAACTA
    ATCGAAAATT CCTACATAAA CTCCATCAAA ACAGTACTCA GGCATCTGAG AGAAGAACAG
    CATAGTGTGG TTGCTTACTT ATACGAGGTT AGAACAAATT TCCAGCAGTT TCTATGGCGT
    CCAGATCACA AGCAAGATTT TGTATCTCAA TGGCAGGCTG ATTTCAATGC TCTTCCTGAT
    GACCTGTGGG ATGATGAAGA AACAAAGTCT GAACTACACC AAAGAGTAAA TGATTTAAGA
    GACCGCCTGT GGGACATCTG TGATGCCCGG AAGGAAGAAG CAGAGCAGGA GCGTCAGGAT
    ATCATTAACG AGAGCTGGTT GCAAGACTCA ATCGGAATAA CGATGAACCA TTTCTTCTCA
    CTAATGCAGA CAGAACTGAA CCGTTTCCAA GATACAAAGA GACTCCTTCA AGATTATTAC
    AGGGGAATGG AATGCAAAAT TCCAATCGAT GACAGTAAGA GATTTACTCG AATCCCGTTG
    GTCCAGCTGG ATAGTAGTAG AGACATTTTG GAAAGCCAAC TCAGAATTCC TCTAATTCCA
    CGAATATCCA TTTCCCCGGA TTCCGCTATG ACCAAACCAA AAACAAAGGC CATACTAAAG
    GGCAAGATCG ATTACTCGCT GGAAAATGTG GAGTTAAACT TTGAGGCAGA TGAGAAGTTG
    GTGATGGACA CCTGGCAGCA GGCTTCTTTA GCAATATCTC ACATGGTGGC TGCTGAAATT
    CATCAGAGGC TCATGGAAGA AGAAAAGGAA AACCAACCAG CAGACACAAA AGAAAAATCT
    CCTCAGATGG GGACAAATAA AAAAGCCAAA GAGGAACCAC CCAAGAAAGA AAAGGCAGAC
    AAAAAAGCAA AAGGCAGATC CCCGCCGACA GCAGAAGCCA CGCCTGTGAC AGTCACAGCA
    GAGGAGATTA CCCAAAAGGA GAAGAGAAAC GAACTGATGC TCAAAATCAA GGAAGAGCAC
    CTCGCTGCCC TGCAGTTTGA AGAGATAGCC ACACAGTTCC GGCTTGAACT GATAAAGACA
    AAAGCATTGG CTTTCCTTGA AGATTTACTA ACAAAGGTGG TCGATGTATA TAAACTCTTG
    GAAAAGTGGC TTGGTGAGAG GTACTTGAAT GAAATGGCCA GTGTGGAAAA ATTAACTGAG
    GTGGCCCGTT ATCACATTGA AACATCAACA AAAATTCAGA ATGAACTTTA CCTAGACCAA
    GAAGATTTCT TCATTAATGG AGATCTGAAA GTCTTTCCAG ATCCCCCTCC CCCAGTGCGC
    CCCCCACCTG TGGAAAAGGA AGAAAATGGC ACCCTGACCA TCGAACAGCT TGACAGTCTT
    AAAGATCAGT TCTTAGATAT TGCACCCAAA GGCATAATAG GACATAAAGC ATTTACTGAC
    ATTCTGCTGG ATCTGGTAAC CCTGAACCTT GGAACAAACA ACTTACCTAG TAGTTGGATG
    CACCTCACGC AACCAGAATT GCAGGAGTTA ACATCTTTAG TAATAGTAAA TTCGGAGTTT
    GTGGACTGGC GGAAATTCCT ATTAGTAATC TCGATGCCTT GGCCCATCCC CATGGAGGAA
    GAGCTCCTCG AAACCCTGCA GAGGTTCAAG GCGGTGGATG AAGAGCAGTT GGGCACCATC
    ACTTTTGAGC AGTATATGCA GGCTGGTCTG TGGTTTACAG GAGATGAATA TATAAAAATT
    CCAGAAAATC CTCTTGAACC TCTGCCGTTC AATAGGCAGG AGCATCTTAT AGAGTTTTTC
    TTTAGGCTAT TTGCTGACCA TGAGAAGGAT CCACCTCAAC TTGACTACAC ACAGATGCTG
    CTCTATTTTG CTTGCCATCC AGATGCTATG GAAGGGGTCT ACAGGGCCCT CAGTGTGGCC
    ATAGGAACTC ATGTCTTCCA ACCAGTCGAA ACTATTGCTA TTATTGGAGA AAAGTCCTCC
    TCCTTGACTG ATGCAAGTCC AGTTGAGGAG TATCCTGAAC TCGAGGGAAA CTTTATGCAT
    GAGGAAGGAG AATTAAAAGA AGACAGGGAG GAAAAGGAAG TAGAAATCCC TGAGAATGCA
    AACACTGAAA AGGTATCCCT GGAAAGACTA CTCAAAGTGT TCCAAGGAGG AAAAGAAGTA
    CAGGATGCTA ACAGATTCAC CAGCCACCTA CAGATAGAGA ACACTTACTC AGAGAACTTC
    GTAAAAGTAT TTCAAGACCT AGGTGCCAAG GATCTGGAGC CAGTTGAAGT TGCTGTTCTC
    TTGAAGCATC CTTTTATTCA AGACCTGATT TCAAATTATT CAGACTATAA AATCCCTGAT
    ATTAAAATAA TTCTCCAAAG GAGTGAGCAT GTACAAGGAA GTGATGGAGA AAGATCTCCT
    TCAAGACTTA CAGAGGAAAA GAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273636.1
    CDS433..5379
    Translation

    Target ORF information:

    RefSeq Version XM_022417928.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris sperm flagellar protein 2 (LOC489235), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417928.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCAAACTA TGCAACCCCT GGCAAACGTC AGATACCAAA GTATGAAAAG TGAGGCTTTT 
    CGAGATCAAC GCCTGAGCAG GAGACGACAA AATGAAATAA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC TGCATCAAAT CGTACTTTGA GAGCACTGGA GGCCCAAAAA
    ATGATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AGATTAAGAA GTTTGAAGCT
    TTAATAAAAA AGGATCTCCA GGCCAAAGAA AGTGCCTCCA AGACTTCTTT AGATACAGCA
    GGCCAGACAA CTACTGATTT GTTAAACACT TATTCGGATG ATGACTACAT TAAAAAAATC
    CAAAAGCGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTATTAATGG ACCAGTTAAT AGCCCACGAA GCACAAGAGG AGGCGTATCG GGAGGAGCAG
    CTGATTAACC GGCTGATGCG GCAGTCCCAG CAGGAGCGCA GGATCTCTGT GCAGCTCATG
    CATGTTCGGC ATGAAAAGGA AGTTTTGTGG CAAAACAGAA TTTTCCGAGA AAAACAGTAT
    GAGGAAAGAC GACTTAAAGA TTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTGGCAAAA
    CAAGCCAAGA TTGATTTTCA GGAACAAGCC ATTAGAGAGA AGGAAATTCA TGAACAGATC
    ACTGTGGAAA GAGCTCAAGC TCGTTACAAA AAGCATTATA CAATATGTGC AGAAATTTTG
    GATCAAATAC TTGATCTTTC CACTAAAGTG TCAGACTACC GGATGTTGAC AAATAATTTG
    ATTCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TTAATGGAAA GCCCATATAC
    AAGCAAGCCT CTATTAAGCA ACTACCAGCC ACGCCCTCTG CAGAACAAGT TATAGAACTG
    GAGAAGAGGG ACTTGCTGGA TAGCAATGAT TATGAAGAAT ATAAGAATAT GGTTGGAGAG
    TGGGCTTTAC CAGAAGAAAT GGTTGATAAT TTACCACCCT CCAACAATAC CATATTGGGT
    CATGTGATTC ACAGATTAAT TGAAAAATCT CATCCTCCTC AAGTCAAATC AACAGTACTA
    GAATTACCTT CATTTGCTAT TAAAGGGTGC TTAGTTGGAA AAACATTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTACA AAAAGACTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTCCAAGAAG CAATCAAAGC ATTCCATGAC AATGAAAAAA TCAGTGAGGC TCTACCAATT
    GAGAAAGAAG CTGAAGAAAA GTCTCTACCA GTTCTGCAAG AGAAGAACAA AGAAAGCCAG
    GATCTACAGA ATGCATTTTC ACCTCATCCA GTTTCAGAAG AAGCGTTACC AGAAACAGAA
    GATAAAACTA TACTTAGTAC TGATACAAAT AAAACTCCAA AAGCTGAAGA AGTCAAATCA
    AGTGATAGTT TCTCTGAACT GACTGTGCGG GCTCAGCTTG GTGCAAAATC AGAAAAGATG
    CTGAAGAAAG GAAAGAGCAT TTCTGACATG TTGCTAGTTA ATATCTTGGT AAATGCTATT
    AATGAGGTAC CTGTGGATCA AGGCTGGGTC TTGGATGGTT TTCCAATGAC GTTAAACCAA
    GCAAAGCTTC TGGAGGAAGC TCTCACGGGC TACAACAGAG AGGCCCTAGA ACTGGAAGAA
    AAAAAAACAC AAATACCCAC ATTGGCTGTT GATGCTAGCA CCTCCAAAGA AGTGCCTCTT
    CCCCCTTCTG CATTTGACTT TGTCATGTTA TTAGATATTT CAGATAATTC CTTGCTGGAC
    CGCATGAATT ACACAATGGC TGAAGCATTT TCAAGTGAAA CTTCTCATGA AGACATCACT
    CAACGTGTAA CTGCTGAAAA GCAAGATATG AATGAGGACC AGAATTTAAG AGACCAGATT
    CAGCACAGAA TTATAGGCTT CTTGGACAAC TGGCCTTTGT TGGAGCAATG GTTTACAGAG
    CCAGAAAATA TTTTGATAAA TATCAATGCT GAAGTAGATA AAGAGTCTTT ATGCAATAAA
    GTAAAAGAAA TGTTTATGAC TGAAATAATG AAAAAAGAGA ATAAAGTTAA AAAGAAATTA
    GAAGAAAAGG AAGCTGAGAA AAAAGAAGTT CCCCTGGGTG AGCCTCCGCC TCCTCCTCCT
    CCCCCTCCCC CTCCTCCTCC TGAGCCAGAA AAAGAGAAGG AAACCCATCC TCAACGAGAG
    CATTCAAAAA CTCCTACTGC AAAAGGGAAA CCACCAGCAG AACCCTTGAC TGGTAAGCCA
    GACTCTCTTC AGGAAGGAAA AGGAAAGAAA GGGGACACTT CACTCAAAAG AAAAGGTTCT
    CCTAAAGGAA AATCACCAGG AGGGAAAGGA CCACTAAAGA AATCACCTGC TGATTCTACA
    GACATGTCAC CTGTTCCCAT AGTGCCACCA CCACCCAAGC CAGGATCAGA AGAATGGGTC
    TACGTGAATG AACCAATTCC TGAGGAAATG CCTTCATTTT TAGTGCCTTA CTGGGAACTA
    ATCGAAAATT CCTACATAAA CTCCATCAAA ACAGTACTCA GGCATCTGAG AGAAGAACAG
    CATAGTGTGG TTGCTTACTT ATACGAGGTT AGAACAAATT TCCAGCAGTT TCTATGGCGT
    CCAGATCACA AGCAAGATTT TGTATCTCAA TGGCAGGCTG ATTTCAATGC TCTTCCTGAT
    GACCTGTGGG ATGATGAAGA AACAAAGTCT GAACTACACC AAAGAGTAAA TGATTTAAGA
    GACCGCCTGT GGGACATCTG TGATGCCCGG AAGGAAGAAG CAGAGCAGGA GCGTCAGGAT
    ATCATTAACG AGAGCTGGTT GCAAGACTCA ATCGGAATAA CGATGAACCA TTTCTTCTCA
    CTAATGCAGA CAGAACTGAA CCGTTTCCAA GATACAAAGA GACTCCTTCA AGATTATTAC
    AGGGGAATGG AATGCAAAAT TCCAATCGAT GACAGTAAGA GATTTACTCG AATCCCGTTG
    GTCCAGCTGG ATAGTAGTAG AGACATTTTG GAAAGCCAAC TCAGAATTCC TCTAATTCCA
    CGAATATCCA TTTCCCCGGA TTCCGCTATG ACCAAACCAA AAACAAAGGC CATACTAAAG
    GGCAAGATCG ATTACTCGCT GGAAAATGTG GAGTTAAACT TTGAGGCAGA TGAGAAGTTG
    GTGATGGACA CCTGGCAGCA GGCTTCTTTA GCAATATCTC ACATGGTGGC TGCTGAAATT
    CATCAGAGGC TCATGGAAGA AGAAAAGGAA AACCAACCAG CAGACACAAA AGAAAAATCT
    CCTCAGATGG GGACAAATAA AAAAGCCAAA GAGGAACCAC CCAAGAAAGA AAAGGCAGAC
    AAAAAAGCAA AAGGCAGATC CCCGCCGACA GCAGAAGCCA CGCCTGTGAC AGTCACAGCA
    GAGGAGATTA CCCAAAAGGA GAAGAGAAAC GAACTGATGC TCAAAATCAA GGAAGAGCAC
    CTCGCTGCCC TGCAGTTTGA AGAGATAGCC ACACAGTTCC GGCTTGAACT GATAAAGACA
    AAAGCATTGG CTTTCCTTGA AGATTTACTA ACAAAGGTGG TCGATGTATA TAAACTCTTG
    GAAAAGTGGC TTGGTGAGAG GTACTTGAAT GAAATGGCCA GTGTGGAAAA ATTAACTGAG
    GTGGCCCGTT ATCACATTGA AACATCAACA AAAATTCAGA ATGAACTTTA CCTAGACCAA
    GAAGATTTCT TCATTAATGG AGATCTGAAA GTCTTTCCAG ATCCCCCTCC CCCAGTGCGC
    CCCCCACCTG TGGAAAAGGA AGAAAATGGC ACCCTGACCA TCGAACAGCT TGACAGTCTT
    AAAGATCAGT TCTTAGATAT TGCACCCAAA GGCATAATAG GACATAAAGC ATTTACTGAC
    ATTCTGCTGG ATCTGGTAAC CCTGAACCTT GGAACAAACA ACTTACCTAG TAGTTGGATG
    CACCTCACGC AACCAGAATT GCAGGAGTTA ACATCTTTAG TAATAGTAAA TTCGGAGTTT
    GTGGACTGGC GGAAATTCCT ATTAGTAATC TCGATGCCTT GGCCCATCCC CATGGAGGAA
    GAGCTCCTCG AAACCCTGCA GAGGTTCAAG GCGGTGGATG AAGAGCAGTT GGGCACCATC
    ACTTTTGAGC AGTATATGCA GGCTGGTCTG TGGTTTACAG GAGATGAATA TATAAAAATT
    CCAGAAAATC CTCTTGAACC TCTGCCGTTC AATAGGCAGG AGCATCTTAT AGAGTTTTTC
    TTTAGGCTAT TTGCTGACCA TGAGAAGGAT CCACCTCAAC TTGACTACAC ACAGATGCTG
    CTCTATTTTG CTTGCCATCC AGATGCTATG GAAGGGGTCT ACAGGGCCCT CAGTGTGGCC
    ATAGGAACTC ATGTCTTCCA ACCAGTCGAA ACTATTGCTA TTATTGGAGA AAAGTCCTCC
    TCCTTGACTG ATGCAAGTCC AGTTGAGGAG TATCCTGAAC TCGAGGGAAA CTTTATGCAT
    GAGGAAGGAG AATTAAAAGA AGACAGGGAG GAAAAGGAAG TAGAAATCCC TGAGAATGCA
    AACACTGAAA AGGTATCCCT GGAAAGACTA CTCAAAGTGT TCCAAGGAGG AAAAGAAGTA
    CAGGATGCTA ACAGATTCAC CAGCCACCTA CAGATAGAGA ACACTTACTC AGAGAACTTC
    GTAAAAGTAT TTCAAGACCT AGGTGCCAAG GATCTGGAGC CAGTTGAAGT TGCTGTTCTC
    TTGAAGCATC CTTTTATTCA AGACCTGATT TCAAATTATT CAGACTATAA AATCCCTGAT
    ATTAAAATAA TTCTCCAAAG GAGTGAGCAT GTACAAGGAA GTGATGGAGA AAGATCTCCT
    TCAAGACTTA CAGAGGAAAA GAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287739
    Clone ID Related Accession (Same CDS sequence) XM_022417925.1 , XM_022417927.1 , XM_022417928.1 , XM_022417926.1
    Accession Version XM_022417926.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product sperm flagellar protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCAAACTA TGCAACCCCT GGCAAACGTC AGATACCAAA GTATGAAAAG TGAGGCTTTT 
    CGAGATCAAC GCCTGAGCAG GAGACGACAA AATGAAATAA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC TGCATCAAAT CGTACTTTGA GAGCACTGGA GGCCCAAAAA
    ATGATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AGATTAAGAA GTTTGAAGCT
    TTAATAAAAA AGGATCTCCA GGCCAAAGAA AGTGCCTCCA AGACTTCTTT AGATACAGCA
    GGCCAGACAA CTACTGATTT GTTAAACACT TATTCGGATG ATGACTACAT TAAAAAAATC
    CAAAAGCGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTATTAATGG ACCAGTTAAT AGCCCACGAA GCACAAGAGG AGGCGTATCG GGAGGAGCAG
    CTGATTAACC GGCTGATGCG GCAGTCCCAG CAGGAGCGCA GGATCTCTGT GCAGCTCATG
    CATGTTCGGC ATGAAAAGGA AGTTTTGTGG CAAAACAGAA TTTTCCGAGA AAAACAGTAT
    GAGGAAAGAC GACTTAAAGA TTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTGGCAAAA
    CAAGCCAAGA TTGATTTTCA GGAACAAGCC ATTAGAGAGA AGGAAATTCA TGAACAGATC
    ACTGTGGAAA GAGCTCAAGC TCGTTACAAA AAGCATTATA CAATATGTGC AGAAATTTTG
    GATCAAATAC TTGATCTTTC CACTAAAGTG TCAGACTACC GGATGTTGAC AAATAATTTG
    ATTCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TTAATGGAAA GCCCATATAC
    AAGCAAGCCT CTATTAAGCA ACTACCAGCC ACGCCCTCTG CAGAACAAGT TATAGAACTG
    GAGAAGAGGG ACTTGCTGGA TAGCAATGAT TATGAAGAAT ATAAGAATAT GGTTGGAGAG
    TGGGCTTTAC CAGAAGAAAT GGTTGATAAT TTACCACCCT CCAACAATAC CATATTGGGT
    CATGTGATTC ACAGATTAAT TGAAAAATCT CATCCTCCTC AAGTCAAATC AACAGTACTA
    GAATTACCTT CATTTGCTAT TAAAGGGTGC TTAGTTGGAA AAACATTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTACA AAAAGACTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTCCAAGAAG CAATCAAAGC ATTCCATGAC AATGAAAAAA TCAGTGAGGC TCTACCAATT
    GAGAAAGAAG CTGAAGAAAA GTCTCTACCA GTTCTGCAAG AGAAGAACAA AGAAAGCCAG
    GATCTACAGA ATGCATTTTC ACCTCATCCA GTTTCAGAAG AAGCGTTACC AGAAACAGAA
    GATAAAACTA TACTTAGTAC TGATACAAAT AAAACTCCAA AAGCTGAAGA AGTCAAATCA
    AGTGATAGTT TCTCTGAACT GACTGTGCGG GCTCAGCTTG GTGCAAAATC AGAAAAGATG
    CTGAAGAAAG GAAAGAGCAT TTCTGACATG TTGCTAGTTA ATATCTTGGT AAATGCTATT
    AATGAGGTAC CTGTGGATCA AGGCTGGGTC TTGGATGGTT TTCCAATGAC GTTAAACCAA
    GCAAAGCTTC TGGAGGAAGC TCTCACGGGC TACAACAGAG AGGCCCTAGA ACTGGAAGAA
    AAAAAAACAC AAATACCCAC ATTGGCTGTT GATGCTAGCA CCTCCAAAGA AGTGCCTCTT
    CCCCCTTCTG CATTTGACTT TGTCATGTTA TTAGATATTT CAGATAATTC CTTGCTGGAC
    CGCATGAATT ACACAATGGC TGAAGCATTT TCAAGTGAAA CTTCTCATGA AGACATCACT
    CAACGTGTAA CTGCTGAAAA GCAAGATATG AATGAGGACC AGAATTTAAG AGACCAGATT
    CAGCACAGAA TTATAGGCTT CTTGGACAAC TGGCCTTTGT TGGAGCAATG GTTTACAGAG
    CCAGAAAATA TTTTGATAAA TATCAATGCT GAAGTAGATA AAGAGTCTTT ATGCAATAAA
    GTAAAAGAAA TGTTTATGAC TGAAATAATG AAAAAAGAGA ATAAAGTTAA AAAGAAATTA
    GAAGAAAAGG AAGCTGAGAA AAAAGAAGTT CCCCTGGGTG AGCCTCCGCC TCCTCCTCCT
    CCCCCTCCCC CTCCTCCTCC TGAGCCAGAA AAAGAGAAGG AAACCCATCC TCAACGAGAG
    CATTCAAAAA CTCCTACTGC AAAAGGGAAA CCACCAGCAG AACCCTTGAC TGGTAAGCCA
    GACTCTCTTC AGGAAGGAAA AGGAAAGAAA GGGGACACTT CACTCAAAAG AAAAGGTTCT
    CCTAAAGGAA AATCACCAGG AGGGAAAGGA CCACTAAAGA AATCACCTGC TGATTCTACA
    GACATGTCAC CTGTTCCCAT AGTGCCACCA CCACCCAAGC CAGGATCAGA AGAATGGGTC
    TACGTGAATG AACCAATTCC TGAGGAAATG CCTTCATTTT TAGTGCCTTA CTGGGAACTA
    ATCGAAAATT CCTACATAAA CTCCATCAAA ACAGTACTCA GGCATCTGAG AGAAGAACAG
    CATAGTGTGG TTGCTTACTT ATACGAGGTT AGAACAAATT TCCAGCAGTT TCTATGGCGT
    CCAGATCACA AGCAAGATTT TGTATCTCAA TGGCAGGCTG ATTTCAATGC TCTTCCTGAT
    GACCTGTGGG ATGATGAAGA AACAAAGTCT GAACTACACC AAAGAGTAAA TGATTTAAGA
    GACCGCCTGT GGGACATCTG TGATGCCCGG AAGGAAGAAG CAGAGCAGGA GCGTCAGGAT
    ATCATTAACG AGAGCTGGTT GCAAGACTCA ATCGGAATAA CGATGAACCA TTTCTTCTCA
    CTAATGCAGA CAGAACTGAA CCGTTTCCAA GATACAAAGA GACTCCTTCA AGATTATTAC
    AGGGGAATGG AATGCAAAAT TCCAATCGAT GACAGTAAGA GATTTACTCG AATCCCGTTG
    GTCCAGCTGG ATAGTAGTAG AGACATTTTG GAAAGCCAAC TCAGAATTCC TCTAATTCCA
    CGAATATCCA TTTCCCCGGA TTCCGCTATG ACCAAACCAA AAACAAAGGC CATACTAAAG
    GGCAAGATCG ATTACTCGCT GGAAAATGTG GAGTTAAACT TTGAGGCAGA TGAGAAGTTG
    GTGATGGACA CCTGGCAGCA GGCTTCTTTA GCAATATCTC ACATGGTGGC TGCTGAAATT
    CATCAGAGGC TCATGGAAGA AGAAAAGGAA AACCAACCAG CAGACACAAA AGAAAAATCT
    CCTCAGATGG GGACAAATAA AAAAGCCAAA GAGGAACCAC CCAAGAAAGA AAAGGCAGAC
    AAAAAAGCAA AAGGCAGATC CCCGCCGACA GCAGAAGCCA CGCCTGTGAC AGTCACAGCA
    GAGGAGATTA CCCAAAAGGA GAAGAGAAAC GAACTGATGC TCAAAATCAA GGAAGAGCAC
    CTCGCTGCCC TGCAGTTTGA AGAGATAGCC ACACAGTTCC GGCTTGAACT GATAAAGACA
    AAAGCATTGG CTTTCCTTGA AGATTTACTA ACAAAGGTGG TCGATGTATA TAAACTCTTG
    GAAAAGTGGC TTGGTGAGAG GTACTTGAAT GAAATGGCCA GTGTGGAAAA ATTAACTGAG
    GTGGCCCGTT ATCACATTGA AACATCAACA AAAATTCAGA ATGAACTTTA CCTAGACCAA
    GAAGATTTCT TCATTAATGG AGATCTGAAA GTCTTTCCAG ATCCCCCTCC CCCAGTGCGC
    CCCCCACCTG TGGAAAAGGA AGAAAATGGC ACCCTGACCA TCGAACAGCT TGACAGTCTT
    AAAGATCAGT TCTTAGATAT TGCACCCAAA GGCATAATAG GACATAAAGC ATTTACTGAC
    ATTCTGCTGG ATCTGGTAAC CCTGAACCTT GGAACAAACA ACTTACCTAG TAGTTGGATG
    CACCTCACGC AACCAGAATT GCAGGAGTTA ACATCTTTAG TAATAGTAAA TTCGGAGTTT
    GTGGACTGGC GGAAATTCCT ATTAGTAATC TCGATGCCTT GGCCCATCCC CATGGAGGAA
    GAGCTCCTCG AAACCCTGCA GAGGTTCAAG GCGGTGGATG AAGAGCAGTT GGGCACCATC
    ACTTTTGAGC AGTATATGCA GGCTGGTCTG TGGTTTACAG GAGATGAATA TATAAAAATT
    CCAGAAAATC CTCTTGAACC TCTGCCGTTC AATAGGCAGG AGCATCTTAT AGAGTTTTTC
    TTTAGGCTAT TTGCTGACCA TGAGAAGGAT CCACCTCAAC TTGACTACAC ACAGATGCTG
    CTCTATTTTG CTTGCCATCC AGATGCTATG GAAGGGGTCT ACAGGGCCCT CAGTGTGGCC
    ATAGGAACTC ATGTCTTCCA ACCAGTCGAA ACTATTGCTA TTATTGGAGA AAAGTCCTCC
    TCCTTGACTG ATGCAAGTCC AGTTGAGGAG TATCCTGAAC TCGAGGGAAA CTTTATGCAT
    GAGGAAGGAG AATTAAAAGA AGACAGGGAG GAAAAGGAAG TAGAAATCCC TGAGAATGCA
    AACACTGAAA AGGTATCCCT GGAAAGACTA CTCAAAGTGT TCCAAGGAGG AAAAGAAGTA
    CAGGATGCTA ACAGATTCAC CAGCCACCTA CAGATAGAGA ACACTTACTC AGAGAACTTC
    GTAAAAGTAT TTCAAGACCT AGGTGCCAAG GATCTGGAGC CAGTTGAAGT TGCTGTTCTC
    TTGAAGCATC CTTTTATTCA AGACCTGATT TCAAATTATT CAGACTATAA AATCCCTGAT
    ATTAAAATAA TTCTCCAAAG GAGTGAGCAT GTACAAGGAA GTGATGGAGA AAGATCTCCT
    TCAAGACTTA CAGAGGAAAA GAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273634.1
    CDS705..5651
    Translation

    Target ORF information:

    RefSeq Version XM_022417926.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris sperm flagellar protein 2 (LOC489235), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417926.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCAAACTA TGCAACCCCT GGCAAACGTC AGATACCAAA GTATGAAAAG TGAGGCTTTT 
    CGAGATCAAC GCCTGAGCAG GAGACGACAA AATGAAATAA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC TGCATCAAAT CGTACTTTGA GAGCACTGGA GGCCCAAAAA
    ATGATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AGATTAAGAA GTTTGAAGCT
    TTAATAAAAA AGGATCTCCA GGCCAAAGAA AGTGCCTCCA AGACTTCTTT AGATACAGCA
    GGCCAGACAA CTACTGATTT GTTAAACACT TATTCGGATG ATGACTACAT TAAAAAAATC
    CAAAAGCGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTATTAATGG ACCAGTTAAT AGCCCACGAA GCACAAGAGG AGGCGTATCG GGAGGAGCAG
    CTGATTAACC GGCTGATGCG GCAGTCCCAG CAGGAGCGCA GGATCTCTGT GCAGCTCATG
    CATGTTCGGC ATGAAAAGGA AGTTTTGTGG CAAAACAGAA TTTTCCGAGA AAAACAGTAT
    GAGGAAAGAC GACTTAAAGA TTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTGGCAAAA
    CAAGCCAAGA TTGATTTTCA GGAACAAGCC ATTAGAGAGA AGGAAATTCA TGAACAGATC
    ACTGTGGAAA GAGCTCAAGC TCGTTACAAA AAGCATTATA CAATATGTGC AGAAATTTTG
    GATCAAATAC TTGATCTTTC CACTAAAGTG TCAGACTACC GGATGTTGAC AAATAATTTG
    ATTCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TTAATGGAAA GCCCATATAC
    AAGCAAGCCT CTATTAAGCA ACTACCAGCC ACGCCCTCTG CAGAACAAGT TATAGAACTG
    GAGAAGAGGG ACTTGCTGGA TAGCAATGAT TATGAAGAAT ATAAGAATAT GGTTGGAGAG
    TGGGCTTTAC CAGAAGAAAT GGTTGATAAT TTACCACCCT CCAACAATAC CATATTGGGT
    CATGTGATTC ACAGATTAAT TGAAAAATCT CATCCTCCTC AAGTCAAATC AACAGTACTA
    GAATTACCTT CATTTGCTAT TAAAGGGTGC TTAGTTGGAA AAACATTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTACA AAAAGACTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTCCAAGAAG CAATCAAAGC ATTCCATGAC AATGAAAAAA TCAGTGAGGC TCTACCAATT
    GAGAAAGAAG CTGAAGAAAA GTCTCTACCA GTTCTGCAAG AGAAGAACAA AGAAAGCCAG
    GATCTACAGA ATGCATTTTC ACCTCATCCA GTTTCAGAAG AAGCGTTACC AGAAACAGAA
    GATAAAACTA TACTTAGTAC TGATACAAAT AAAACTCCAA AAGCTGAAGA AGTCAAATCA
    AGTGATAGTT TCTCTGAACT GACTGTGCGG GCTCAGCTTG GTGCAAAATC AGAAAAGATG
    CTGAAGAAAG GAAAGAGCAT TTCTGACATG TTGCTAGTTA ATATCTTGGT AAATGCTATT
    AATGAGGTAC CTGTGGATCA AGGCTGGGTC TTGGATGGTT TTCCAATGAC GTTAAACCAA
    GCAAAGCTTC TGGAGGAAGC TCTCACGGGC TACAACAGAG AGGCCCTAGA ACTGGAAGAA
    AAAAAAACAC AAATACCCAC ATTGGCTGTT GATGCTAGCA CCTCCAAAGA AGTGCCTCTT
    CCCCCTTCTG CATTTGACTT TGTCATGTTA TTAGATATTT CAGATAATTC CTTGCTGGAC
    CGCATGAATT ACACAATGGC TGAAGCATTT TCAAGTGAAA CTTCTCATGA AGACATCACT
    CAACGTGTAA CTGCTGAAAA GCAAGATATG AATGAGGACC AGAATTTAAG AGACCAGATT
    CAGCACAGAA TTATAGGCTT CTTGGACAAC TGGCCTTTGT TGGAGCAATG GTTTACAGAG
    CCAGAAAATA TTTTGATAAA TATCAATGCT GAAGTAGATA AAGAGTCTTT ATGCAATAAA
    GTAAAAGAAA TGTTTATGAC TGAAATAATG AAAAAAGAGA ATAAAGTTAA AAAGAAATTA
    GAAGAAAAGG AAGCTGAGAA AAAAGAAGTT CCCCTGGGTG AGCCTCCGCC TCCTCCTCCT
    CCCCCTCCCC CTCCTCCTCC TGAGCCAGAA AAAGAGAAGG AAACCCATCC TCAACGAGAG
    CATTCAAAAA CTCCTACTGC AAAAGGGAAA CCACCAGCAG AACCCTTGAC TGGTAAGCCA
    GACTCTCTTC AGGAAGGAAA AGGAAAGAAA GGGGACACTT CACTCAAAAG AAAAGGTTCT
    CCTAAAGGAA AATCACCAGG AGGGAAAGGA CCACTAAAGA AATCACCTGC TGATTCTACA
    GACATGTCAC CTGTTCCCAT AGTGCCACCA CCACCCAAGC CAGGATCAGA AGAATGGGTC
    TACGTGAATG AACCAATTCC TGAGGAAATG CCTTCATTTT TAGTGCCTTA CTGGGAACTA
    ATCGAAAATT CCTACATAAA CTCCATCAAA ACAGTACTCA GGCATCTGAG AGAAGAACAG
    CATAGTGTGG TTGCTTACTT ATACGAGGTT AGAACAAATT TCCAGCAGTT TCTATGGCGT
    CCAGATCACA AGCAAGATTT TGTATCTCAA TGGCAGGCTG ATTTCAATGC TCTTCCTGAT
    GACCTGTGGG ATGATGAAGA AACAAAGTCT GAACTACACC AAAGAGTAAA TGATTTAAGA
    GACCGCCTGT GGGACATCTG TGATGCCCGG AAGGAAGAAG CAGAGCAGGA GCGTCAGGAT
    ATCATTAACG AGAGCTGGTT GCAAGACTCA ATCGGAATAA CGATGAACCA TTTCTTCTCA
    CTAATGCAGA CAGAACTGAA CCGTTTCCAA GATACAAAGA GACTCCTTCA AGATTATTAC
    AGGGGAATGG AATGCAAAAT TCCAATCGAT GACAGTAAGA GATTTACTCG AATCCCGTTG
    GTCCAGCTGG ATAGTAGTAG AGACATTTTG GAAAGCCAAC TCAGAATTCC TCTAATTCCA
    CGAATATCCA TTTCCCCGGA TTCCGCTATG ACCAAACCAA AAACAAAGGC CATACTAAAG
    GGCAAGATCG ATTACTCGCT GGAAAATGTG GAGTTAAACT TTGAGGCAGA TGAGAAGTTG
    GTGATGGACA CCTGGCAGCA GGCTTCTTTA GCAATATCTC ACATGGTGGC TGCTGAAATT
    CATCAGAGGC TCATGGAAGA AGAAAAGGAA AACCAACCAG CAGACACAAA AGAAAAATCT
    CCTCAGATGG GGACAAATAA AAAAGCCAAA GAGGAACCAC CCAAGAAAGA AAAGGCAGAC
    AAAAAAGCAA AAGGCAGATC CCCGCCGACA GCAGAAGCCA CGCCTGTGAC AGTCACAGCA
    GAGGAGATTA CCCAAAAGGA GAAGAGAAAC GAACTGATGC TCAAAATCAA GGAAGAGCAC
    CTCGCTGCCC TGCAGTTTGA AGAGATAGCC ACACAGTTCC GGCTTGAACT GATAAAGACA
    AAAGCATTGG CTTTCCTTGA AGATTTACTA ACAAAGGTGG TCGATGTATA TAAACTCTTG
    GAAAAGTGGC TTGGTGAGAG GTACTTGAAT GAAATGGCCA GTGTGGAAAA ATTAACTGAG
    GTGGCCCGTT ATCACATTGA AACATCAACA AAAATTCAGA ATGAACTTTA CCTAGACCAA
    GAAGATTTCT TCATTAATGG AGATCTGAAA GTCTTTCCAG ATCCCCCTCC CCCAGTGCGC
    CCCCCACCTG TGGAAAAGGA AGAAAATGGC ACCCTGACCA TCGAACAGCT TGACAGTCTT
    AAAGATCAGT TCTTAGATAT TGCACCCAAA GGCATAATAG GACATAAAGC ATTTACTGAC
    ATTCTGCTGG ATCTGGTAAC CCTGAACCTT GGAACAAACA ACTTACCTAG TAGTTGGATG
    CACCTCACGC AACCAGAATT GCAGGAGTTA ACATCTTTAG TAATAGTAAA TTCGGAGTTT
    GTGGACTGGC GGAAATTCCT ATTAGTAATC TCGATGCCTT GGCCCATCCC CATGGAGGAA
    GAGCTCCTCG AAACCCTGCA GAGGTTCAAG GCGGTGGATG AAGAGCAGTT GGGCACCATC
    ACTTTTGAGC AGTATATGCA GGCTGGTCTG TGGTTTACAG GAGATGAATA TATAAAAATT
    CCAGAAAATC CTCTTGAACC TCTGCCGTTC AATAGGCAGG AGCATCTTAT AGAGTTTTTC
    TTTAGGCTAT TTGCTGACCA TGAGAAGGAT CCACCTCAAC TTGACTACAC ACAGATGCTG
    CTCTATTTTG CTTGCCATCC AGATGCTATG GAAGGGGTCT ACAGGGCCCT CAGTGTGGCC
    ATAGGAACTC ATGTCTTCCA ACCAGTCGAA ACTATTGCTA TTATTGGAGA AAAGTCCTCC
    TCCTTGACTG ATGCAAGTCC AGTTGAGGAG TATCCTGAAC TCGAGGGAAA CTTTATGCAT
    GAGGAAGGAG AATTAAAAGA AGACAGGGAG GAAAAGGAAG TAGAAATCCC TGAGAATGCA
    AACACTGAAA AGGTATCCCT GGAAAGACTA CTCAAAGTGT TCCAAGGAGG AAAAGAAGTA
    CAGGATGCTA ACAGATTCAC CAGCCACCTA CAGATAGAGA ACACTTACTC AGAGAACTTC
    GTAAAAGTAT TTCAAGACCT AGGTGCCAAG GATCTGGAGC CAGTTGAAGT TGCTGTTCTC
    TTGAAGCATC CTTTTATTCA AGACCTGATT TCAAATTATT CAGACTATAA AATCCCTGAT
    ATTAAAATAA TTCTCCAAAG GAGTGAGCAT GTACAAGGAA GTGATGGAGA AAGATCTCCT
    TCAAGACTTA CAGAGGAAAA GAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287740
    Clone ID Related Accession (Same CDS sequence) XM_022417930.1 , XM_022417929.1
    Accession Version XM_022417930.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product sperm flagellar protein 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCCAAAA TCCAAGCAGC TATCATACAG ATTCCTAAAC CTGCATCAAA TCGTACTTTG 
    AGAGCACTGG AGGCCCAAAA AATGATGAAA AAGAAAAAAG AGGCAGAGGA TGTGGCCAAT
    GAGATTAAGA AGTTTGAAGC TTTAATAAAA AAGGATCTCC AGGCCAAAGA AAGTGCCTCC
    AAGACTTCTT TAGATACAGC AGGCCAGACA ACTACTGATT TGTTAAACAC TTATTCGGAT
    GATGACTACA TTAAAAAAAT CCAAAAGCGC CTTGAAGAAG ATGCTTTTGC ACGAGAGCAA
    AGGGAGAAAA GACGGCGGAG ACTATTAATG GACCAGTTAA TAGCCCACGA AGCACAAGAG
    GAGGCGTATC GGGAGGAGCA GCTGATTAAC CGGCTGATGC GGCAGTCCCA GCAGGAGCGC
    AGGATCTCTG TGCAGCTCAT GCATGTTCGG CATGAAAAGG AAGTTTTGTG GCAAAACAGA
    ATTTTCCGAG AAAAACAGTA TGAGGAAAGA CGACTTAAAG ATTTCCAGGA TGCTCTTGAT
    CGAGAAGCGG CTTTGGCAAA ACAAGCCAAG ATTGATTTTC AGGAACAAGC CATTAGAGAG
    AAGGAAATTC ATGAACAGAT CACTGTGGAA AGAGCTCAAG CTCGTTACAA AAAGCATTAT
    ACAATATGTG CAGAAATTTT GGATCAAATA CTTGATCTTT CCACTAAAGT GTCAGACTAC
    CGGATGTTGA CAAATAATTT GATTCCATAT AAGTTGATGC ATGATTGGAA GGAACTGTTT
    TTTAATGGAA AGCCCATATA CAAGCAAGCC TCTATTAAGC AACTACCAGC CACGCCCTCT
    GCAGAACAAG TTATAGAACT GGAGAAGAGG GACTTGCTGG ATAGCAATGA TTATGAAGAA
    TATAAGAATA TGGTTGGAGA GTGGGCTTTA CCAGAAGAAA TGGTTGATAA TTTACCACCC
    TCCAACAATA CCATATTGGG TCATGTGATT CACAGATTAA TTGAAAAATC TCATCCTCCT
    CAAGTCAAAT CAACAGTACT AGAATTACCT TCATTTGCTA TTAAAGGGTG CTTAGTTGGA
    AAAACATTTA GTGGAAAAAC TACTGCTCTA AAGGCTCTAC AAAAAGACTT TCCTATTCAG
    GTACTTTCTA TTGACACTCT TGTCCAAGAA GCAATCAAAG CATTCCATGA CAATGAAAAA
    ATCAGTGAGG CTCTACCAAT TGAGAAAGAA GCTGAAGAAA AGTCTCTACC AGTTCTGCAA
    GAGAAGAACA AAGAAAGCCA GGATCTACAG AATGCATTTT CACCTCATCC AGTTTCAGAA
    GAAGCGTTAC CAGAAACAGA AGATAAAACT ATACTTAGTA CTGATACAAA TAAAACTCCA
    AAAGCTGAAG AAGTCAAATC AAGTGATAGT TTCTCTGAAC TGACTGTGCG GGCTCAGCTT
    GGTGCAAAAT CAGAAAAGAT GCTGAAGAAA GGAAAGAGCA TTTCTGACAT GTTGCTAGTT
    AATATCTTGG TAAATGCTAT TAATGAGGTA CCTGTGGATC AAGGCTGGGT CTTGGATGGT
    TTTCCAATGA CGTTAAACCA AGCAAAGCTT CTGGAGGAAG CTCTCACGGG CTACAACAGA
    GAGGCCCTAG AACTGGAAGA AAAAAAAACA CAAATACCCA CATTGGCTGT TGATGCTAGC
    ACCTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGACT TTGTCATGTT ATTAGATATT
    TCAGATAATT CCTTGCTGGA CCGCATGAAT TACACAATGG CTGAAGCATT TTCAAGTGAA
    ACTTCTCATG AAGACATCAC TCAACGTGTA ACTGCTGAAA AGCAAGATAT GAATGAGGAC
    CAGAATTTAA GAGACCAGAT TCAGCACAGA ATTATAGGCT TCTTGGACAA CTGGCCTTTG
    TTGGAGCAAT GGTTTACAGA GCCAGAAAAT ATTTTGATAA ATATCAATGC TGAAGTAGAT
    AAAGAGTCTT TATGCAATAA AGTAAAAGAA ATGTTTATGA CTGAAATAAT GAAAAAAGAG
    AATAAAGTTA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA AAAAAGAAGT TCCCCTGGGT
    GAGCCTCCGC CTCCTCCTCC TCCCCCTCCC CCTCCTCCTC CTGAGCCAGA AAAAGAGAAG
    GAAACCCATC CTCAACGAGA GCATTCAAAA ACTCCTACTG CAAAAGGGAA ACCACCAGCA
    GAACCCTTGA CTGGTAAGCC AGACTCTCTT CAGGAAGGAA AAGGAAAGAA AGGGGACACT
    TCACTCAAAA GAAAAGGTTC TCCTAAAGGA AAATCACCAG GAGGGAAAGG ACCACTAAAG
    AAATCACCTG CTGATTCTAC AGACATGTCA CCTGTTCCCA TAGTGCCACC ACCACCCAAG
    CCAGGATCAG AAGAATGGGT CTACGTGAAT GAACCAATTC CTGAGGAAAT GCCTTCATTT
    TTAGTGCCTT ACTGGGAACT AATCGAAAAT TCCTACATAA ACTCCATCAA AACAGTACTC
    AGGCATCTGA GAGAAGAACA GCATAGTGTG GTTGCTTACT TATACGAGGT TAGAACAAAT
    TTCCAGCAGT TTCTATGGCG TCCAGATCAC AAGCAAGATT TTGTATCTCA ATGGCAGGCT
    GATTTCAATG CTCTTCCTGA TGACCTGTGG GATGATGAAG AAACAAAGTC TGAACTACAC
    CAAAGAGTAA ATGATTTAAG AGACCGCCTG TGGGACATCT GTGATGCCCG GAAGGAAGAA
    GCAGAGCAGG AGCGTCAGGA TATCATTAAC GAGAGCTGGT TGCAAGACTC AATCGGAATA
    ACGATGAACC ATTTCTTCTC ACTAATGCAG ACAGAACTGA ACCGTTTCCA AGATACAAAG
    AGACTCCTTC AAGATTATTA CAGGGGAATG GAATGCAAAA TTCCAATCGA TGACAGTAAG
    AGATTTACTC GAATCCCGTT GGTCCAGCTG GATAGTAGTA GAGACATTTT GGAAAGCCAA
    CTCAGAATTC CTCTAATTCC ACGAATATCC ATTTCCCCGG ATTCCGCTAT GACCAAACCA
    AAAACAAAGG CCATACTAAA GGGCAAGATC GATTACTCGC TGGAAAATGT GGAGTTAAAC
    TTTGAGGCAG ATGAGAAGTT GGTGATGGAC ACCTGGCAGC AGGCTTCTTT AGCAATATCT
    CACATGGTGG CTGCTGAAAT TCATCAGAGG CTCATGGAAG AAGAAAAGGA AAACCAACCA
    GCAGACACAA AAGAAAAATC TCCTCAGATG GGGACAAATA AAAAAGCCAA AGAGGAACCA
    CCCAAGAAAG AAAAGGCAGA CAAAAAAGCA AAAGGCAGAT CCCCGCCGAC AGCAGAAGCC
    ACGCCTGTGA CAGTCACAGC AGAGGAGATT ACCCAAAAGG AGAAGAGAAA CGAACTGATG
    CTCAAAATCA AGGAAGAGCA CCTCGCTGCC CTGCAGTTTG AAGAGATAGC CACACAGTTC
    CGGCTTGAAC TGATAAAGAC AAAAGCATTG GCTTTCCTTG AAGATTTACT AACAAAGGTG
    GTCGATGTAT ATAAACTCTT GGAAAAGTGG CTTGGTGAGA GGTACTTGAA TGAAATGGCC
    AGTGTGGAAA AATTAACTGA GGTGGCCCGT TATCACATTG AAACATCAAC AAAAATTCAG
    AATGAACTTT ACCTAGACCA AGAAGATTTC TTCATTAATG GAGATCTGAA AGTCTTTCCA
    GATCCCCCTC CCCCAGTGCG CCCCCCACCT GTGGAAAAGG AAGAAAATGG CACCCTGACC
    ATCGAACAGC TTGACAGTCT TAAAGATCAG TTCTTAGATA TTGCACCCAA AGGCATAATA
    GGACATAAAG CATTTACTGA CATTCTGCTG GATCTGGTAA CCCTGAACCT TGGAACAAAC
    AACTTACCTA GTAGTTGGAT GCACCTCACG CAACCAGAAT TGCAGGAGTT AACATCTTTA
    GTAATAGTAA ATTCGGAGTT TGTGGACTGG CGGAAATTCC TATTAGTAAT CTCGATGCCT
    TGGCCCATCC CCATGGAGGA AGAGCTCCTC GAAACCCTGC AGAGGTTCAA GGCGGTGGAT
    GAAGAGCAGT TGGGCACCAT CACTTTTGAG CAGTATATGC AGGCTGGTCT GTGGTTTACA
    GGAGATGAAT ATATAAAAAT TCCAGAAAAT CCTCTTGAAC CTCTGCCGTT CAATAGGCAG
    GAGCATCTTA TAGAGTTTTT CTTTAGGCTA TTTGCTGACC ATGAGAAGGA TCCACCTCAA
    CTTGACTACA CACAGATGCT GCTCTATTTT GCTTGCCATC CAGATGCTAT GGAAGGGGTC
    TACAGGGCCC TCAGTGTGGC CATAGGAACT CATGTCTTCC AACCAGTCGA AACTATTGCT
    ATTATTGGAG AAAAGTCCTC CTCCTTGACT GATGCAAGTC CAGTTGAGGA GTATCCTGAA
    CTCGAGGGAA ACTTTATGCA TGAGGAAGGA GAATTAAAAG AAGACAGGGA GGAAAAGGAA
    GTAGAAATCC CTGAGAATGC AAACACTGAA AAGGTATCCC TGGAAAGACT ACTCAAAGTG
    TTCCAAGGAG GAAAAGAAGT ACAGGATGCT AACAGATTCA CCAGCCACCT ACAGATAGAG
    AACACTTACT CAGAGAACTT CGTAAAAGTA TTTCAAGACC TAGGTGCCAA GGATCTGGAG
    CCAGTTGAAG TTGCTGTTCT CTTGAAGCAT CCTTTTATTC AAGACCTGAT TTCAAATTAT
    TCAGACTATA AAATCCCTGA TATTAAAATA ATTCTCCAAA GGAGTGAGCA TGTACAAGGA
    AGTGATGGAG AAAGATCTCC TTCAAGACTT ACAGAGGAAA AGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273638.1
    CDS155..5002
    Translation

    Target ORF information:

    RefSeq Version XM_022417930.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris sperm flagellar protein 2 (LOC489235), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417930.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCCAAAA TCCAAGCAGC TATCATACAG ATTCCTAAAC CTGCATCAAA TCGTACTTTG 
    AGAGCACTGG AGGCCCAAAA AATGATGAAA AAGAAAAAAG AGGCAGAGGA TGTGGCCAAT
    GAGATTAAGA AGTTTGAAGC TTTAATAAAA AAGGATCTCC AGGCCAAAGA AAGTGCCTCC
    AAGACTTCTT TAGATACAGC AGGCCAGACA ACTACTGATT TGTTAAACAC TTATTCGGAT
    GATGACTACA TTAAAAAAAT CCAAAAGCGC CTTGAAGAAG ATGCTTTTGC ACGAGAGCAA
    AGGGAGAAAA GACGGCGGAG ACTATTAATG GACCAGTTAA TAGCCCACGA AGCACAAGAG
    GAGGCGTATC GGGAGGAGCA GCTGATTAAC CGGCTGATGC GGCAGTCCCA GCAGGAGCGC
    AGGATCTCTG TGCAGCTCAT GCATGTTCGG CATGAAAAGG AAGTTTTGTG GCAAAACAGA
    ATTTTCCGAG AAAAACAGTA TGAGGAAAGA CGACTTAAAG ATTTCCAGGA TGCTCTTGAT
    CGAGAAGCGG CTTTGGCAAA ACAAGCCAAG ATTGATTTTC AGGAACAAGC CATTAGAGAG
    AAGGAAATTC ATGAACAGAT CACTGTGGAA AGAGCTCAAG CTCGTTACAA AAAGCATTAT
    ACAATATGTG CAGAAATTTT GGATCAAATA CTTGATCTTT CCACTAAAGT GTCAGACTAC
    CGGATGTTGA CAAATAATTT GATTCCATAT AAGTTGATGC ATGATTGGAA GGAACTGTTT
    TTTAATGGAA AGCCCATATA CAAGCAAGCC TCTATTAAGC AACTACCAGC CACGCCCTCT
    GCAGAACAAG TTATAGAACT GGAGAAGAGG GACTTGCTGG ATAGCAATGA TTATGAAGAA
    TATAAGAATA TGGTTGGAGA GTGGGCTTTA CCAGAAGAAA TGGTTGATAA TTTACCACCC
    TCCAACAATA CCATATTGGG TCATGTGATT CACAGATTAA TTGAAAAATC TCATCCTCCT
    CAAGTCAAAT CAACAGTACT AGAATTACCT TCATTTGCTA TTAAAGGGTG CTTAGTTGGA
    AAAACATTTA GTGGAAAAAC TACTGCTCTA AAGGCTCTAC AAAAAGACTT TCCTATTCAG
    GTACTTTCTA TTGACACTCT TGTCCAAGAA GCAATCAAAG CATTCCATGA CAATGAAAAA
    ATCAGTGAGG CTCTACCAAT TGAGAAAGAA GCTGAAGAAA AGTCTCTACC AGTTCTGCAA
    GAGAAGAACA AAGAAAGCCA GGATCTACAG AATGCATTTT CACCTCATCC AGTTTCAGAA
    GAAGCGTTAC CAGAAACAGA AGATAAAACT ATACTTAGTA CTGATACAAA TAAAACTCCA
    AAAGCTGAAG AAGTCAAATC AAGTGATAGT TTCTCTGAAC TGACTGTGCG GGCTCAGCTT
    GGTGCAAAAT CAGAAAAGAT GCTGAAGAAA GGAAAGAGCA TTTCTGACAT GTTGCTAGTT
    AATATCTTGG TAAATGCTAT TAATGAGGTA CCTGTGGATC AAGGCTGGGT CTTGGATGGT
    TTTCCAATGA CGTTAAACCA AGCAAAGCTT CTGGAGGAAG CTCTCACGGG CTACAACAGA
    GAGGCCCTAG AACTGGAAGA AAAAAAAACA CAAATACCCA CATTGGCTGT TGATGCTAGC
    ACCTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGACT TTGTCATGTT ATTAGATATT
    TCAGATAATT CCTTGCTGGA CCGCATGAAT TACACAATGG CTGAAGCATT TTCAAGTGAA
    ACTTCTCATG AAGACATCAC TCAACGTGTA ACTGCTGAAA AGCAAGATAT GAATGAGGAC
    CAGAATTTAA GAGACCAGAT TCAGCACAGA ATTATAGGCT TCTTGGACAA CTGGCCTTTG
    TTGGAGCAAT GGTTTACAGA GCCAGAAAAT ATTTTGATAA ATATCAATGC TGAAGTAGAT
    AAAGAGTCTT TATGCAATAA AGTAAAAGAA ATGTTTATGA CTGAAATAAT GAAAAAAGAG
    AATAAAGTTA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA AAAAAGAAGT TCCCCTGGGT
    GAGCCTCCGC CTCCTCCTCC TCCCCCTCCC CCTCCTCCTC CTGAGCCAGA AAAAGAGAAG
    GAAACCCATC CTCAACGAGA GCATTCAAAA ACTCCTACTG CAAAAGGGAA ACCACCAGCA
    GAACCCTTGA CTGGTAAGCC AGACTCTCTT CAGGAAGGAA AAGGAAAGAA AGGGGACACT
    TCACTCAAAA GAAAAGGTTC TCCTAAAGGA AAATCACCAG GAGGGAAAGG ACCACTAAAG
    AAATCACCTG CTGATTCTAC AGACATGTCA CCTGTTCCCA TAGTGCCACC ACCACCCAAG
    CCAGGATCAG AAGAATGGGT CTACGTGAAT GAACCAATTC CTGAGGAAAT GCCTTCATTT
    TTAGTGCCTT ACTGGGAACT AATCGAAAAT TCCTACATAA ACTCCATCAA AACAGTACTC
    AGGCATCTGA GAGAAGAACA GCATAGTGTG GTTGCTTACT TATACGAGGT TAGAACAAAT
    TTCCAGCAGT TTCTATGGCG TCCAGATCAC AAGCAAGATT TTGTATCTCA ATGGCAGGCT
    GATTTCAATG CTCTTCCTGA TGACCTGTGG GATGATGAAG AAACAAAGTC TGAACTACAC
    CAAAGAGTAA ATGATTTAAG AGACCGCCTG TGGGACATCT GTGATGCCCG GAAGGAAGAA
    GCAGAGCAGG AGCGTCAGGA TATCATTAAC GAGAGCTGGT TGCAAGACTC AATCGGAATA
    ACGATGAACC ATTTCTTCTC ACTAATGCAG ACAGAACTGA ACCGTTTCCA AGATACAAAG
    AGACTCCTTC AAGATTATTA CAGGGGAATG GAATGCAAAA TTCCAATCGA TGACAGTAAG
    AGATTTACTC GAATCCCGTT GGTCCAGCTG GATAGTAGTA GAGACATTTT GGAAAGCCAA
    CTCAGAATTC CTCTAATTCC ACGAATATCC ATTTCCCCGG ATTCCGCTAT GACCAAACCA
    AAAACAAAGG CCATACTAAA GGGCAAGATC GATTACTCGC TGGAAAATGT GGAGTTAAAC
    TTTGAGGCAG ATGAGAAGTT GGTGATGGAC ACCTGGCAGC AGGCTTCTTT AGCAATATCT
    CACATGGTGG CTGCTGAAAT TCATCAGAGG CTCATGGAAG AAGAAAAGGA AAACCAACCA
    GCAGACACAA AAGAAAAATC TCCTCAGATG GGGACAAATA AAAAAGCCAA AGAGGAACCA
    CCCAAGAAAG AAAAGGCAGA CAAAAAAGCA AAAGGCAGAT CCCCGCCGAC AGCAGAAGCC
    ACGCCTGTGA CAGTCACAGC AGAGGAGATT ACCCAAAAGG AGAAGAGAAA CGAACTGATG
    CTCAAAATCA AGGAAGAGCA CCTCGCTGCC CTGCAGTTTG AAGAGATAGC CACACAGTTC
    CGGCTTGAAC TGATAAAGAC AAAAGCATTG GCTTTCCTTG AAGATTTACT AACAAAGGTG
    GTCGATGTAT ATAAACTCTT GGAAAAGTGG CTTGGTGAGA GGTACTTGAA TGAAATGGCC
    AGTGTGGAAA AATTAACTGA GGTGGCCCGT TATCACATTG AAACATCAAC AAAAATTCAG
    AATGAACTTT ACCTAGACCA AGAAGATTTC TTCATTAATG GAGATCTGAA AGTCTTTCCA
    GATCCCCCTC CCCCAGTGCG CCCCCCACCT GTGGAAAAGG AAGAAAATGG CACCCTGACC
    ATCGAACAGC TTGACAGTCT TAAAGATCAG TTCTTAGATA TTGCACCCAA AGGCATAATA
    GGACATAAAG CATTTACTGA CATTCTGCTG GATCTGGTAA CCCTGAACCT TGGAACAAAC
    AACTTACCTA GTAGTTGGAT GCACCTCACG CAACCAGAAT TGCAGGAGTT AACATCTTTA
    GTAATAGTAA ATTCGGAGTT TGTGGACTGG CGGAAATTCC TATTAGTAAT CTCGATGCCT
    TGGCCCATCC CCATGGAGGA AGAGCTCCTC GAAACCCTGC AGAGGTTCAA GGCGGTGGAT
    GAAGAGCAGT TGGGCACCAT CACTTTTGAG CAGTATATGC AGGCTGGTCT GTGGTTTACA
    GGAGATGAAT ATATAAAAAT TCCAGAAAAT CCTCTTGAAC CTCTGCCGTT CAATAGGCAG
    GAGCATCTTA TAGAGTTTTT CTTTAGGCTA TTTGCTGACC ATGAGAAGGA TCCACCTCAA
    CTTGACTACA CACAGATGCT GCTCTATTTT GCTTGCCATC CAGATGCTAT GGAAGGGGTC
    TACAGGGCCC TCAGTGTGGC CATAGGAACT CATGTCTTCC AACCAGTCGA AACTATTGCT
    ATTATTGGAG AAAAGTCCTC CTCCTTGACT GATGCAAGTC CAGTTGAGGA GTATCCTGAA
    CTCGAGGGAA ACTTTATGCA TGAGGAAGGA GAATTAAAAG AAGACAGGGA GGAAAAGGAA
    GTAGAAATCC CTGAGAATGC AAACACTGAA AAGGTATCCC TGGAAAGACT ACTCAAAGTG
    TTCCAAGGAG GAAAAGAAGT ACAGGATGCT AACAGATTCA CCAGCCACCT ACAGATAGAG
    AACACTTACT CAGAGAACTT CGTAAAAGTA TTTCAAGACC TAGGTGCCAA GGATCTGGAG
    CCAGTTGAAG TTGCTGTTCT CTTGAAGCAT CCTTTTATTC AAGACCTGAT TTCAAATTAT
    TCAGACTATA AAATCCCTGA TATTAAAATA ATTCTCCAAA GGAGTGAGCA TGTACAAGGA
    AGTGATGGAG AAAGATCTCC TTCAAGACTT ACAGAGGAAA AGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287740
    Clone ID Related Accession (Same CDS sequence) XM_022417930.1 , XM_022417929.1
    Accession Version XM_022417929.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product sperm flagellar protein 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCCAAAA TCCAAGCAGC TATCATACAG ATTCCTAAAC CTGCATCAAA TCGTACTTTG 
    AGAGCACTGG AGGCCCAAAA AATGATGAAA AAGAAAAAAG AGGCAGAGGA TGTGGCCAAT
    GAGATTAAGA AGTTTGAAGC TTTAATAAAA AAGGATCTCC AGGCCAAAGA AAGTGCCTCC
    AAGACTTCTT TAGATACAGC AGGCCAGACA ACTACTGATT TGTTAAACAC TTATTCGGAT
    GATGACTACA TTAAAAAAAT CCAAAAGCGC CTTGAAGAAG ATGCTTTTGC ACGAGAGCAA
    AGGGAGAAAA GACGGCGGAG ACTATTAATG GACCAGTTAA TAGCCCACGA AGCACAAGAG
    GAGGCGTATC GGGAGGAGCA GCTGATTAAC CGGCTGATGC GGCAGTCCCA GCAGGAGCGC
    AGGATCTCTG TGCAGCTCAT GCATGTTCGG CATGAAAAGG AAGTTTTGTG GCAAAACAGA
    ATTTTCCGAG AAAAACAGTA TGAGGAAAGA CGACTTAAAG ATTTCCAGGA TGCTCTTGAT
    CGAGAAGCGG CTTTGGCAAA ACAAGCCAAG ATTGATTTTC AGGAACAAGC CATTAGAGAG
    AAGGAAATTC ATGAACAGAT CACTGTGGAA AGAGCTCAAG CTCGTTACAA AAAGCATTAT
    ACAATATGTG CAGAAATTTT GGATCAAATA CTTGATCTTT CCACTAAAGT GTCAGACTAC
    CGGATGTTGA CAAATAATTT GATTCCATAT AAGTTGATGC ATGATTGGAA GGAACTGTTT
    TTTAATGGAA AGCCCATATA CAAGCAAGCC TCTATTAAGC AACTACCAGC CACGCCCTCT
    GCAGAACAAG TTATAGAACT GGAGAAGAGG GACTTGCTGG ATAGCAATGA TTATGAAGAA
    TATAAGAATA TGGTTGGAGA GTGGGCTTTA CCAGAAGAAA TGGTTGATAA TTTACCACCC
    TCCAACAATA CCATATTGGG TCATGTGATT CACAGATTAA TTGAAAAATC TCATCCTCCT
    CAAGTCAAAT CAACAGTACT AGAATTACCT TCATTTGCTA TTAAAGGGTG CTTAGTTGGA
    AAAACATTTA GTGGAAAAAC TACTGCTCTA AAGGCTCTAC AAAAAGACTT TCCTATTCAG
    GTACTTTCTA TTGACACTCT TGTCCAAGAA GCAATCAAAG CATTCCATGA CAATGAAAAA
    ATCAGTGAGG CTCTACCAAT TGAGAAAGAA GCTGAAGAAA AGTCTCTACC AGTTCTGCAA
    GAGAAGAACA AAGAAAGCCA GGATCTACAG AATGCATTTT CACCTCATCC AGTTTCAGAA
    GAAGCGTTAC CAGAAACAGA AGATAAAACT ATACTTAGTA CTGATACAAA TAAAACTCCA
    AAAGCTGAAG AAGTCAAATC AAGTGATAGT TTCTCTGAAC TGACTGTGCG GGCTCAGCTT
    GGTGCAAAAT CAGAAAAGAT GCTGAAGAAA GGAAAGAGCA TTTCTGACAT GTTGCTAGTT
    AATATCTTGG TAAATGCTAT TAATGAGGTA CCTGTGGATC AAGGCTGGGT CTTGGATGGT
    TTTCCAATGA CGTTAAACCA AGCAAAGCTT CTGGAGGAAG CTCTCACGGG CTACAACAGA
    GAGGCCCTAG AACTGGAAGA AAAAAAAACA CAAATACCCA CATTGGCTGT TGATGCTAGC
    ACCTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGACT TTGTCATGTT ATTAGATATT
    TCAGATAATT CCTTGCTGGA CCGCATGAAT TACACAATGG CTGAAGCATT TTCAAGTGAA
    ACTTCTCATG AAGACATCAC TCAACGTGTA ACTGCTGAAA AGCAAGATAT GAATGAGGAC
    CAGAATTTAA GAGACCAGAT TCAGCACAGA ATTATAGGCT TCTTGGACAA CTGGCCTTTG
    TTGGAGCAAT GGTTTACAGA GCCAGAAAAT ATTTTGATAA ATATCAATGC TGAAGTAGAT
    AAAGAGTCTT TATGCAATAA AGTAAAAGAA ATGTTTATGA CTGAAATAAT GAAAAAAGAG
    AATAAAGTTA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA AAAAAGAAGT TCCCCTGGGT
    GAGCCTCCGC CTCCTCCTCC TCCCCCTCCC CCTCCTCCTC CTGAGCCAGA AAAAGAGAAG
    GAAACCCATC CTCAACGAGA GCATTCAAAA ACTCCTACTG CAAAAGGGAA ACCACCAGCA
    GAACCCTTGA CTGGTAAGCC AGACTCTCTT CAGGAAGGAA AAGGAAAGAA AGGGGACACT
    TCACTCAAAA GAAAAGGTTC TCCTAAAGGA AAATCACCAG GAGGGAAAGG ACCACTAAAG
    AAATCACCTG CTGATTCTAC AGACATGTCA CCTGTTCCCA TAGTGCCACC ACCACCCAAG
    CCAGGATCAG AAGAATGGGT CTACGTGAAT GAACCAATTC CTGAGGAAAT GCCTTCATTT
    TTAGTGCCTT ACTGGGAACT AATCGAAAAT TCCTACATAA ACTCCATCAA AACAGTACTC
    AGGCATCTGA GAGAAGAACA GCATAGTGTG GTTGCTTACT TATACGAGGT TAGAACAAAT
    TTCCAGCAGT TTCTATGGCG TCCAGATCAC AAGCAAGATT TTGTATCTCA ATGGCAGGCT
    GATTTCAATG CTCTTCCTGA TGACCTGTGG GATGATGAAG AAACAAAGTC TGAACTACAC
    CAAAGAGTAA ATGATTTAAG AGACCGCCTG TGGGACATCT GTGATGCCCG GAAGGAAGAA
    GCAGAGCAGG AGCGTCAGGA TATCATTAAC GAGAGCTGGT TGCAAGACTC AATCGGAATA
    ACGATGAACC ATTTCTTCTC ACTAATGCAG ACAGAACTGA ACCGTTTCCA AGATACAAAG
    AGACTCCTTC AAGATTATTA CAGGGGAATG GAATGCAAAA TTCCAATCGA TGACAGTAAG
    AGATTTACTC GAATCCCGTT GGTCCAGCTG GATAGTAGTA GAGACATTTT GGAAAGCCAA
    CTCAGAATTC CTCTAATTCC ACGAATATCC ATTTCCCCGG ATTCCGCTAT GACCAAACCA
    AAAACAAAGG CCATACTAAA GGGCAAGATC GATTACTCGC TGGAAAATGT GGAGTTAAAC
    TTTGAGGCAG ATGAGAAGTT GGTGATGGAC ACCTGGCAGC AGGCTTCTTT AGCAATATCT
    CACATGGTGG CTGCTGAAAT TCATCAGAGG CTCATGGAAG AAGAAAAGGA AAACCAACCA
    GCAGACACAA AAGAAAAATC TCCTCAGATG GGGACAAATA AAAAAGCCAA AGAGGAACCA
    CCCAAGAAAG AAAAGGCAGA CAAAAAAGCA AAAGGCAGAT CCCCGCCGAC AGCAGAAGCC
    ACGCCTGTGA CAGTCACAGC AGAGGAGATT ACCCAAAAGG AGAAGAGAAA CGAACTGATG
    CTCAAAATCA AGGAAGAGCA CCTCGCTGCC CTGCAGTTTG AAGAGATAGC CACACAGTTC
    CGGCTTGAAC TGATAAAGAC AAAAGCATTG GCTTTCCTTG AAGATTTACT AACAAAGGTG
    GTCGATGTAT ATAAACTCTT GGAAAAGTGG CTTGGTGAGA GGTACTTGAA TGAAATGGCC
    AGTGTGGAAA AATTAACTGA GGTGGCCCGT TATCACATTG AAACATCAAC AAAAATTCAG
    AATGAACTTT ACCTAGACCA AGAAGATTTC TTCATTAATG GAGATCTGAA AGTCTTTCCA
    GATCCCCCTC CCCCAGTGCG CCCCCCACCT GTGGAAAAGG AAGAAAATGG CACCCTGACC
    ATCGAACAGC TTGACAGTCT TAAAGATCAG TTCTTAGATA TTGCACCCAA AGGCATAATA
    GGACATAAAG CATTTACTGA CATTCTGCTG GATCTGGTAA CCCTGAACCT TGGAACAAAC
    AACTTACCTA GTAGTTGGAT GCACCTCACG CAACCAGAAT TGCAGGAGTT AACATCTTTA
    GTAATAGTAA ATTCGGAGTT TGTGGACTGG CGGAAATTCC TATTAGTAAT CTCGATGCCT
    TGGCCCATCC CCATGGAGGA AGAGCTCCTC GAAACCCTGC AGAGGTTCAA GGCGGTGGAT
    GAAGAGCAGT TGGGCACCAT CACTTTTGAG CAGTATATGC AGGCTGGTCT GTGGTTTACA
    GGAGATGAAT ATATAAAAAT TCCAGAAAAT CCTCTTGAAC CTCTGCCGTT CAATAGGCAG
    GAGCATCTTA TAGAGTTTTT CTTTAGGCTA TTTGCTGACC ATGAGAAGGA TCCACCTCAA
    CTTGACTACA CACAGATGCT GCTCTATTTT GCTTGCCATC CAGATGCTAT GGAAGGGGTC
    TACAGGGCCC TCAGTGTGGC CATAGGAACT CATGTCTTCC AACCAGTCGA AACTATTGCT
    ATTATTGGAG AAAAGTCCTC CTCCTTGACT GATGCAAGTC CAGTTGAGGA GTATCCTGAA
    CTCGAGGGAA ACTTTATGCA TGAGGAAGGA GAATTAAAAG AAGACAGGGA GGAAAAGGAA
    GTAGAAATCC CTGAGAATGC AAACACTGAA AAGGTATCCC TGGAAAGACT ACTCAAAGTG
    TTCCAAGGAG GAAAAGAAGT ACAGGATGCT AACAGATTCA CCAGCCACCT ACAGATAGAG
    AACACTTACT CAGAGAACTT CGTAAAAGTA TTTCAAGACC TAGGTGCCAA GGATCTGGAG
    CCAGTTGAAG TTGCTGTTCT CTTGAAGCAT CCTTTTATTC AAGACCTGAT TTCAAATTAT
    TCAGACTATA AAATCCCTGA TATTAAAATA ATTCTCCAAA GGAGTGAGCA TGTACAAGGA
    AGTGATGGAG AAAGATCTCC TTCAAGACTT ACAGAGGAAA AGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273637.1
    CDS424..5271
    Translation

    Target ORF information:

    RefSeq Version XM_022417929.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris sperm flagellar protein 2 (LOC489235), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417929.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCCAAAA TCCAAGCAGC TATCATACAG ATTCCTAAAC CTGCATCAAA TCGTACTTTG 
    AGAGCACTGG AGGCCCAAAA AATGATGAAA AAGAAAAAAG AGGCAGAGGA TGTGGCCAAT
    GAGATTAAGA AGTTTGAAGC TTTAATAAAA AAGGATCTCC AGGCCAAAGA AAGTGCCTCC
    AAGACTTCTT TAGATACAGC AGGCCAGACA ACTACTGATT TGTTAAACAC TTATTCGGAT
    GATGACTACA TTAAAAAAAT CCAAAAGCGC CTTGAAGAAG ATGCTTTTGC ACGAGAGCAA
    AGGGAGAAAA GACGGCGGAG ACTATTAATG GACCAGTTAA TAGCCCACGA AGCACAAGAG
    GAGGCGTATC GGGAGGAGCA GCTGATTAAC CGGCTGATGC GGCAGTCCCA GCAGGAGCGC
    AGGATCTCTG TGCAGCTCAT GCATGTTCGG CATGAAAAGG AAGTTTTGTG GCAAAACAGA
    ATTTTCCGAG AAAAACAGTA TGAGGAAAGA CGACTTAAAG ATTTCCAGGA TGCTCTTGAT
    CGAGAAGCGG CTTTGGCAAA ACAAGCCAAG ATTGATTTTC AGGAACAAGC CATTAGAGAG
    AAGGAAATTC ATGAACAGAT CACTGTGGAA AGAGCTCAAG CTCGTTACAA AAAGCATTAT
    ACAATATGTG CAGAAATTTT GGATCAAATA CTTGATCTTT CCACTAAAGT GTCAGACTAC
    CGGATGTTGA CAAATAATTT GATTCCATAT AAGTTGATGC ATGATTGGAA GGAACTGTTT
    TTTAATGGAA AGCCCATATA CAAGCAAGCC TCTATTAAGC AACTACCAGC CACGCCCTCT
    GCAGAACAAG TTATAGAACT GGAGAAGAGG GACTTGCTGG ATAGCAATGA TTATGAAGAA
    TATAAGAATA TGGTTGGAGA GTGGGCTTTA CCAGAAGAAA TGGTTGATAA TTTACCACCC
    TCCAACAATA CCATATTGGG TCATGTGATT CACAGATTAA TTGAAAAATC TCATCCTCCT
    CAAGTCAAAT CAACAGTACT AGAATTACCT TCATTTGCTA TTAAAGGGTG CTTAGTTGGA
    AAAACATTTA GTGGAAAAAC TACTGCTCTA AAGGCTCTAC AAAAAGACTT TCCTATTCAG
    GTACTTTCTA TTGACACTCT TGTCCAAGAA GCAATCAAAG CATTCCATGA CAATGAAAAA
    ATCAGTGAGG CTCTACCAAT TGAGAAAGAA GCTGAAGAAA AGTCTCTACC AGTTCTGCAA
    GAGAAGAACA AAGAAAGCCA GGATCTACAG AATGCATTTT CACCTCATCC AGTTTCAGAA
    GAAGCGTTAC CAGAAACAGA AGATAAAACT ATACTTAGTA CTGATACAAA TAAAACTCCA
    AAAGCTGAAG AAGTCAAATC AAGTGATAGT TTCTCTGAAC TGACTGTGCG GGCTCAGCTT
    GGTGCAAAAT CAGAAAAGAT GCTGAAGAAA GGAAAGAGCA TTTCTGACAT GTTGCTAGTT
    AATATCTTGG TAAATGCTAT TAATGAGGTA CCTGTGGATC AAGGCTGGGT CTTGGATGGT
    TTTCCAATGA CGTTAAACCA AGCAAAGCTT CTGGAGGAAG CTCTCACGGG CTACAACAGA
    GAGGCCCTAG AACTGGAAGA AAAAAAAACA CAAATACCCA CATTGGCTGT TGATGCTAGC
    ACCTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGACT TTGTCATGTT ATTAGATATT
    TCAGATAATT CCTTGCTGGA CCGCATGAAT TACACAATGG CTGAAGCATT TTCAAGTGAA
    ACTTCTCATG AAGACATCAC TCAACGTGTA ACTGCTGAAA AGCAAGATAT GAATGAGGAC
    CAGAATTTAA GAGACCAGAT TCAGCACAGA ATTATAGGCT TCTTGGACAA CTGGCCTTTG
    TTGGAGCAAT GGTTTACAGA GCCAGAAAAT ATTTTGATAA ATATCAATGC TGAAGTAGAT
    AAAGAGTCTT TATGCAATAA AGTAAAAGAA ATGTTTATGA CTGAAATAAT GAAAAAAGAG
    AATAAAGTTA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA AAAAAGAAGT TCCCCTGGGT
    GAGCCTCCGC CTCCTCCTCC TCCCCCTCCC CCTCCTCCTC CTGAGCCAGA AAAAGAGAAG
    GAAACCCATC CTCAACGAGA GCATTCAAAA ACTCCTACTG CAAAAGGGAA ACCACCAGCA
    GAACCCTTGA CTGGTAAGCC AGACTCTCTT CAGGAAGGAA AAGGAAAGAA AGGGGACACT
    TCACTCAAAA GAAAAGGTTC TCCTAAAGGA AAATCACCAG GAGGGAAAGG ACCACTAAAG
    AAATCACCTG CTGATTCTAC AGACATGTCA CCTGTTCCCA TAGTGCCACC ACCACCCAAG
    CCAGGATCAG AAGAATGGGT CTACGTGAAT GAACCAATTC CTGAGGAAAT GCCTTCATTT
    TTAGTGCCTT ACTGGGAACT AATCGAAAAT TCCTACATAA ACTCCATCAA AACAGTACTC
    AGGCATCTGA GAGAAGAACA GCATAGTGTG GTTGCTTACT TATACGAGGT TAGAACAAAT
    TTCCAGCAGT TTCTATGGCG TCCAGATCAC AAGCAAGATT TTGTATCTCA ATGGCAGGCT
    GATTTCAATG CTCTTCCTGA TGACCTGTGG GATGATGAAG AAACAAAGTC TGAACTACAC
    CAAAGAGTAA ATGATTTAAG AGACCGCCTG TGGGACATCT GTGATGCCCG GAAGGAAGAA
    GCAGAGCAGG AGCGTCAGGA TATCATTAAC GAGAGCTGGT TGCAAGACTC AATCGGAATA
    ACGATGAACC ATTTCTTCTC ACTAATGCAG ACAGAACTGA ACCGTTTCCA AGATACAAAG
    AGACTCCTTC AAGATTATTA CAGGGGAATG GAATGCAAAA TTCCAATCGA TGACAGTAAG
    AGATTTACTC GAATCCCGTT GGTCCAGCTG GATAGTAGTA GAGACATTTT GGAAAGCCAA
    CTCAGAATTC CTCTAATTCC ACGAATATCC ATTTCCCCGG ATTCCGCTAT GACCAAACCA
    AAAACAAAGG CCATACTAAA GGGCAAGATC GATTACTCGC TGGAAAATGT GGAGTTAAAC
    TTTGAGGCAG ATGAGAAGTT GGTGATGGAC ACCTGGCAGC AGGCTTCTTT AGCAATATCT
    CACATGGTGG CTGCTGAAAT TCATCAGAGG CTCATGGAAG AAGAAAAGGA AAACCAACCA
    GCAGACACAA AAGAAAAATC TCCTCAGATG GGGACAAATA AAAAAGCCAA AGAGGAACCA
    CCCAAGAAAG AAAAGGCAGA CAAAAAAGCA AAAGGCAGAT CCCCGCCGAC AGCAGAAGCC
    ACGCCTGTGA CAGTCACAGC AGAGGAGATT ACCCAAAAGG AGAAGAGAAA CGAACTGATG
    CTCAAAATCA AGGAAGAGCA CCTCGCTGCC CTGCAGTTTG AAGAGATAGC CACACAGTTC
    CGGCTTGAAC TGATAAAGAC AAAAGCATTG GCTTTCCTTG AAGATTTACT AACAAAGGTG
    GTCGATGTAT ATAAACTCTT GGAAAAGTGG CTTGGTGAGA GGTACTTGAA TGAAATGGCC
    AGTGTGGAAA AATTAACTGA GGTGGCCCGT TATCACATTG AAACATCAAC AAAAATTCAG
    AATGAACTTT ACCTAGACCA AGAAGATTTC TTCATTAATG GAGATCTGAA AGTCTTTCCA
    GATCCCCCTC CCCCAGTGCG CCCCCCACCT GTGGAAAAGG AAGAAAATGG CACCCTGACC
    ATCGAACAGC TTGACAGTCT TAAAGATCAG TTCTTAGATA TTGCACCCAA AGGCATAATA
    GGACATAAAG CATTTACTGA CATTCTGCTG GATCTGGTAA CCCTGAACCT TGGAACAAAC
    AACTTACCTA GTAGTTGGAT GCACCTCACG CAACCAGAAT TGCAGGAGTT AACATCTTTA
    GTAATAGTAA ATTCGGAGTT TGTGGACTGG CGGAAATTCC TATTAGTAAT CTCGATGCCT
    TGGCCCATCC CCATGGAGGA AGAGCTCCTC GAAACCCTGC AGAGGTTCAA GGCGGTGGAT
    GAAGAGCAGT TGGGCACCAT CACTTTTGAG CAGTATATGC AGGCTGGTCT GTGGTTTACA
    GGAGATGAAT ATATAAAAAT TCCAGAAAAT CCTCTTGAAC CTCTGCCGTT CAATAGGCAG
    GAGCATCTTA TAGAGTTTTT CTTTAGGCTA TTTGCTGACC ATGAGAAGGA TCCACCTCAA
    CTTGACTACA CACAGATGCT GCTCTATTTT GCTTGCCATC CAGATGCTAT GGAAGGGGTC
    TACAGGGCCC TCAGTGTGGC CATAGGAACT CATGTCTTCC AACCAGTCGA AACTATTGCT
    ATTATTGGAG AAAAGTCCTC CTCCTTGACT GATGCAAGTC CAGTTGAGGA GTATCCTGAA
    CTCGAGGGAA ACTTTATGCA TGAGGAAGGA GAATTAAAAG AAGACAGGGA GGAAAAGGAA
    GTAGAAATCC CTGAGAATGC AAACACTGAA AAGGTATCCC TGGAAAGACT ACTCAAAGTG
    TTCCAAGGAG GAAAAGAAGT ACAGGATGCT AACAGATTCA CCAGCCACCT ACAGATAGAG
    AACACTTACT CAGAGAACTT CGTAAAAGTA TTTCAAGACC TAGGTGCCAA GGATCTGGAG
    CCAGTTGAAG TTGCTGTTCT CTTGAAGCAT CCTTTTATTC AAGACCTGAT TTCAAATTAT
    TCAGACTATA AAATCCCTGA TATTAAAATA ATTCTCCAAA GGAGTGAGCA TGTACAAGGA
    AGTGATGGAG AAAGATCTCC TTCAAGACTT ACAGAGGAAA AGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36161
    Clone ID Related Accession (Same CDS sequence) XM_014113142.1
    Accession Version XM_014113142.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5928bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product sperm flagellar protein 2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCAAACTA TGCAACCCCT GGCAAACGTC AGATACCAAA GTATGAAAAG TGAGGCTTTT 
    CGAGATCAAC GCCTGAGCAG GAGACGACAA AATGAAATAA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC TGCATCAAAT CGTACTTTGA GAGCACTGGA GGCCCAAAAA
    ATGATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AGATTAAGAA GTTTGAAGCT
    TTAATAAAAA AGGATCTCCA GGCCAAAGAA AGTGCCTCCA AGACTTCTTT AGATACAGCA
    GGCCAGACAA CTACTGATTT GTTAAACACT TATTCGGATG ATGACTACAT TAAAAAAATC
    CAAAAGCGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTATTAATGG ACCAGTTAAT AGCCCACGAA GCACAAGAGG AGGCGTATCG GGAGGAGCAG
    CTGATTAACC GGCTGATGCG GCAGTCCCAG CAGGAGCGCA GGATCTCTGT GCAGCTCATG
    CATGTTCGGC ATGAAAAGGA AGTTTTGTGG CAAAACAGAA TTTTCCGAGA AAAACAGTAT
    GAGGAAAGAC GACTTAAAGA TTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTGGCAAAA
    CAAGCCAAGA TTGATTTTCA GGAACAAGCC ATTAGAGAGA AGGAAATTCA TGAACAGATC
    ACTGTGGAAA GAGCTCAAGC TCGTTACAAA AAGCATTATA CAATATGTGC AGAAATTTTG
    GATCAAATAC TTGATCTTTC CACTAAAGTG TCAGACTACC GGATGTTGAC AAATAATTTG
    ATTCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TTAATGGAAA GCCCATATAC
    AAGCAAGCCT CTATTAAGCA ACTACCAGCC ACGCCCTCTG CAGAACAAGT TATAGAACTG
    GAGAAGAGGG ACTTGCTGGA TAGCAATGAT TATGAAGAAT ATAAGAATAT GGTTGGAGAG
    TGGGCTTTAC CAGAAGAAAT GGTTGATAAT TTACCACCCT CCAACAATAC CATATTGGGT
    CATGTGATTC ACAGATTAAT TGAAAAATCT CATCCTCCTC AAGTCAAATC AACAGTACTA
    GAATTACCTT CATTTGCTAT TAAAGGGTGC TTAGTTGGAA AAACATTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTACA AAAAGACTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTCCAAGAAG CAATCAAAGC ATTCCATGAC AATGAAAAAA TCAGTGAGGC TCTACCAATT
    GAGAAAGAAG CTGAAGAAAA GTCTCTACCA GTTCTGCAAG AGAAGAACAA AGAAAGCCAG
    GATCTACAGA ATGCATTTTC ACCTCATCCA GTTTCAGAAG AAGCGTTACC AGAAACAGAA
    GATAAAACTA TACTTAGTAC TGATACAAAT AAAACTCCAA AAGCTGAAGA AGTCAAATCA
    AGTGATAGTT TCTCTGAACT GACTGTGCGG GCTCAGCTTG GTGCAAAATC AGAAAAGATG
    CTGAAGAAAG GAAAGAGCAT TTCTGACATG TTGCTAGTTA ATATCTTGGT AAATGCTATT
    AATGAGGTAC CTGTGGATCA AGGCTGGGTC TTGGATGGTT TTCCAATGAC GTTAAACCAA
    GCAAAGCTTC TGGAGGAAGC TCTCACGGGC TACAACAGAG AGGCCCTAGA ACTGGAAGAA
    AAAAAAACAC AAATACCCAC ATTGGCTGTT GATGCTAGCA CCTCCAAAGA AGTGCCTCTT
    CCCCCTTCTG CATTTGACTT TGTCATGTTA TTAGATATTT CAGATAATTC CTTGCTGGAC
    CGCATGAATT ACACAATGGC TGAAGCATTT TCAAGTGAAA CTTCTCATGA AGACATCACT
    CAACGTGTAA CTGCTGAAAA GCAAGATATG AATGAGGACC AGAATTTAAG AGACCAGATT
    CAGCACAGAA TTATAGGCTT CTTGGACAAC TGGCCTTTGT TGGAGCAATG GTTTACAGAG
    CCAGAAAATA TTTTGATAAA TATCAATGCT GAAGTAGATA AAGAGTCTTT ATGCAATAAA
    GTAAAAGAAA TGTTTATGAC TGAAATAATG AAAAAAGAGA ATAAAGTTAA AAAGAAATTA
    GAAGAAAAGG AAGCTGAGAA AAAAGAAGTT CCCCTGGGTG AGCCTCCGCC TCCTCCTCCT
    CCCCCTCCCC CTCCTCCTCC TGAGCCAGAA AAAGAGAAGG AAACCCATCC TCAACGAGAG
    CATTCAAAAA CTCCTACTGC AAAAGGGAAA CCACCAGCAG GTTCTCCTAA AGGAAAATCA
    CCAGGAGGGA AAGGACCACT AAAGAAATCA CCTGCTGATT CTACAGACAT GTCACCTGTT
    CCCATAGTGC CACCACCACC CAAGCCAGGA TCAGAAGAAT GGGTCTACGT GAATGAACCA
    ATTCCTGAGG AAATGCCTTC ATTTTTAGTG CCTTACTGGG AACTAATCGA AAATTCCTAC
    ATAAACTCCA TCAAAACAGT ACTCAGGCAT CTGAGAGAAG AACAGCATAG TGTGGTTGCT
    TACTTATACG AGGTTAGAAC AAATTTCCAG CAGTTTCTAT GGCGTCCAGA TCACAAGCAA
    GATTTTGTAT CTCAATGGCA GGCTGATTTC AATGCTCTTC CTGATGACCT GTGGGATGAT
    GAAGAAACAA AGTCTGAACT ACACCAAAGA GTAAATGATT TAAGAGACCG CCTGTGGGAC
    ATCTGTGATG CCCGGAAGGA AGAAGCAGAG CAGGAGCGTC AGGATATCAT TAACGAGAGC
    TGGTTGCAAG ACTCAATCGG AATAACGATG AACCATTTCT TCTCACTAAT GCAGACAGAA
    CTGAACCGTT TCCAAGATAC AAAGAGACTC CTTCAAGATT ATTACAGGGG AATGGAATGC
    AAAATTCCAA TCGATGACAG TAAGAGATTT ACTCGAATCC CGTTGGTCCA GCTGGATAGT
    AGTAGAGACA TTTTGGAAAG CCAACTCAGA ATTCCTCTAA TTCCACGAAT ATCCATTTCC
    CCGGATTCCG CTATGACCAA ACCAAAAACA AAGGCCATAC TAAAGGGCAA GATCGATTAC
    TCGCTGGAAA ATGTGGAGTT AAACTTTGAG GCAGATGAGA AGTTGGTGAT GGACACCTGG
    CAGCAGGCTT CTTTAGCAAT ATCTCACATG GTGGCTGCTG AAATTCATCA GAGGCTCATG
    GAAGAAGAAA AGGAAAACCA ACCAGCAGAC ACAAAAGAAA AATCTCCTCA GATGGGGACA
    AATAAAAAAG CCAAAGAGGA ACCACCCAAG AAAGAAAAGG CAGACAAAAA AGCAAAAGGC
    AGATCCCCGC CGACAGCAGA AGCCACGCCT GTGACAGTCA CAGCAGAGGA GATTACCCAA
    AAGGAGAAGA GAAACGAACT GATGCTCAAA ATCAAGGAAG AGCACCTCGC TGCCCTGCAG
    TTTGAAGAGA TAGCCACACA GTTCCGGCTT GAACTGATAA AGACAAAAGC ATTGGCTTTC
    CTTGAAGATT TACTAACAAA GGTGGTCGAT GTATATAAAC TCTTGGAAAA GTGGCTTGGT
    GAGAGGTACT TGAATGAAAT GGCCAGTGTG GAAAAATTAA CTGAGGTGGC CCGTTATCAC
    ATTGAAACAT CAACAAAAAT TCAGAATGAA CTTTACCTAG ACCAAGAAGA TTTCTTCATT
    AATGGAGATC TGAAAGTCTT TCCAGATCCC CCTCCCCCAG TGCGCCCCCC ACCTGTGGAA
    AAGGAAGAAA ATGGCACCCT GACCATCGAA CAGCTTGACA GTCTTAAAGA TCAGTTCTTA
    GATATTGCAC CCAAAGGCAG GGATCCTCCA GGTGACATGA ACCGAGTCAC TGAAAAGCAG
    AAAAGGCTGC AGAAGCTTGA CCATTACCTT CCAAAAAGGA ACGATCAATT TTTGAAAATC
    TGGCTGAAAA AATACCCTTG GCTTATATAT GATGAGATGT TAAATCTTAT GTTCTGTGGC
    CTGTGTCGTA AACATGGAGT GAAGTCAAAG GGAAATCAAG TGAGTTTTTT TTATGGCACT
    GACAACTTTA GGACAGAATT TCTCAATGCA CATCATCTCA GTGAGGCTCA TGCCAAGGCT
    TCACTAATGG AAGCAACAAA TGGTTCTCCA GTGAATGTCG CCACCACAGA GCTCATGGTG
    ACAACCATGA GCAAAATCAC CCTCGGGAGA GTAGAGAACT TATTCAGATC ATGCCATGCT
    ATCGCCAAGA CGGGACGTCC TCTCAAAGAC TTTATCTGGA TGTGCAAGTT GGATGATATG
    AAGGGGGTCG ATATCGGCCC CGTATTCAGA ACTAACAAAT CAGCAAGAAC CTTTACATAT
    TTTATTGCTG AAGTGGAACG GAAAAATCTT AGGGAGAAAC TAGAACAAAG TAAATTTTTC
    TCTGTCCTCA GTGACGGAAT CACAGACAGT TCAACCAAGG CGGCTGAACT TGTCTACGTG
    CAGTTTGCAC ACGCGGGAAA AGTGCATTGC CAAATTGCTG GGGTACAGAC AGTAGAAAGG
    GAGGATCCTT TGGCAATAAG AGATGCCATT GAAAGAACTC TAGAGAGAAA TCTTCAACTT
    AAGCTATCAG ACCAAGACTG GGCCAAGAAA CTGGTTGGTT TTGGAAGTGA TGGTGCCCTT
    GGCTCAGGGA GAGAAAACAG CAGGGTGGCC CTGCTTTTAA GAGAAATCCA GCCATGTGCT
    CTGACTGTAT ACTGCTTTGC ACACCATCTT GAACTGTCTT ACAAGGCGGT GTTCCAAAGC
    GTTCCCCTGT ACAACGACGT CAGAAATCTT CTTCACAGCA TCTACCACTT CTACCACAGT
    TCCCCTCTGC ACAGGAGTTC TCTGGTAACT GCTTTCAAAG GCCTTCACCT TCGACCTGTG
    ATGCCTTCTC AAGTAGGGGG CAGGAGGTGG CTTCAGGAAC TACAGGCAGC TCTCCAGAAC
    TTTCTCAAGG GATATCCTGC AATAGTCCAG CAACTGCATT CGGGAGATGA AGGCAGGAGT
    GACACCAGTC ATCAGAAAGC CAAGGAACTC CTGGGGTTCC TTCTCCAAGC AAACATTGTT
    AAATTTATCC ACTTCTTAGT GGATGTCATT AATGTCCTTA GCATTCTTTC CCGTGTCAAT
    CAAAACAGAA ATTCTTCCAT TGCTGATGTA TTTGCCACAT TAGAATCAAC ACTGGAAATG
    CTCAGAATAT ATCAAACAAG ACCAGGACCA AAGGAACGTT GGGTGGATTC GGTCACGCAC
    TTTCATGGCA ACTGCCTGGG AGGAAAGGGA GACATTTCTG ATGTGAGAAG AGTAGTCTTA
    ACACATCTTA TCAAGAGGCT GCAAGGTTGC TTCAGAGATG CCAGCCAAGA TGTGCTGAAG
    GCAACCATGA TTGGAAGCTT TAAACTGTGG CCTGCGAAGA TAAATCAAGA ATTTGGAGAA
    AAAGAGGTAT CCACCCTGAT TGCCCATTAT GAACCAGTAC TGGAAGCTGC CAACGTGGAA
    ATCGACGAAG TGGACGCTGA GTGGAGCATG CTGAAATTAG AGATTTATGC TAGATTTCAG
    AACATCCGCA AATTGACCTG GGATTTTGTG AATTCTGTTT ACTTACACAA GTATCCAAAT
    ATCTTGACGT TAGTGGACCT CGTGCTGACC CTCCCTGCAA GTTCCGCGGA GGCAGAACGT
    GGCTTTAGTC AGATGAAACT CACCAAGTCA CAAATGCGTG CCAAAATCAA GGCTGAAAGT
    ATGACGGATC TCCTTGTAAT TCAGTTGAAT TCTCCGGACA TTAATAACTT TGACCCTCGG
    AAAGCTATCC ATTTATGGAA CACAAGAACA CCATCTTCAG CTGATGACAC AAGACCTAAT
    TCTGCTTATT CATCAAACTC CGAAAGCCAT GATGAAAGCG ATTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968617.1
    CDS207..6134
    Translation

    Target ORF information:

    RefSeq Version XM_014113142.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113142.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCAAACTA TGCAACCCCT GGCAAACGTC AGATACCAAA GTATGAAAAG TGAGGCTTTT 
    CGAGATCAAC GCCTGAGCAG GAGACGACAA AATGAAATAA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC TGCATCAAAT CGTACTTTGA GAGCACTGGA GGCCCAAAAA
    ATGATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AGATTAAGAA GTTTGAAGCT
    TTAATAAAAA AGGATCTCCA GGCCAAAGAA AGTGCCTCCA AGACTTCTTT AGATACAGCA
    GGCCAGACAA CTACTGATTT GTTAAACACT TATTCGGATG ATGACTACAT TAAAAAAATC
    CAAAAGCGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTATTAATGG ACCAGTTAAT AGCCCACGAA GCACAAGAGG AGGCGTATCG GGAGGAGCAG
    CTGATTAACC GGCTGATGCG GCAGTCCCAG CAGGAGCGCA GGATCTCTGT GCAGCTCATG
    CATGTTCGGC ATGAAAAGGA AGTTTTGTGG CAAAACAGAA TTTTCCGAGA AAAACAGTAT
    GAGGAAAGAC GACTTAAAGA TTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTGGCAAAA
    CAAGCCAAGA TTGATTTTCA GGAACAAGCC ATTAGAGAGA AGGAAATTCA TGAACAGATC
    ACTGTGGAAA GAGCTCAAGC TCGTTACAAA AAGCATTATA CAATATGTGC AGAAATTTTG
    GATCAAATAC TTGATCTTTC CACTAAAGTG TCAGACTACC GGATGTTGAC AAATAATTTG
    ATTCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TTAATGGAAA GCCCATATAC
    AAGCAAGCCT CTATTAAGCA ACTACCAGCC ACGCCCTCTG CAGAACAAGT TATAGAACTG
    GAGAAGAGGG ACTTGCTGGA TAGCAATGAT TATGAAGAAT ATAAGAATAT GGTTGGAGAG
    TGGGCTTTAC CAGAAGAAAT GGTTGATAAT TTACCACCCT CCAACAATAC CATATTGGGT
    CATGTGATTC ACAGATTAAT TGAAAAATCT CATCCTCCTC AAGTCAAATC AACAGTACTA
    GAATTACCTT CATTTGCTAT TAAAGGGTGC TTAGTTGGAA AAACATTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTACA AAAAGACTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTCCAAGAAG CAATCAAAGC ATTCCATGAC AATGAAAAAA TCAGTGAGGC TCTACCAATT
    GAGAAAGAAG CTGAAGAAAA GTCTCTACCA GTTCTGCAAG AGAAGAACAA AGAAAGCCAG
    GATCTACAGA ATGCATTTTC ACCTCATCCA GTTTCAGAAG AAGCGTTACC AGAAACAGAA
    GATAAAACTA TACTTAGTAC TGATACAAAT AAAACTCCAA AAGCTGAAGA AGTCAAATCA
    AGTGATAGTT TCTCTGAACT GACTGTGCGG GCTCAGCTTG GTGCAAAATC AGAAAAGATG
    CTGAAGAAAG GAAAGAGCAT TTCTGACATG TTGCTAGTTA ATATCTTGGT AAATGCTATT
    AATGAGGTAC CTGTGGATCA AGGCTGGGTC TTGGATGGTT TTCCAATGAC GTTAAACCAA
    GCAAAGCTTC TGGAGGAAGC TCTCACGGGC TACAACAGAG AGGCCCTAGA ACTGGAAGAA
    AAAAAAACAC AAATACCCAC ATTGGCTGTT GATGCTAGCA CCTCCAAAGA AGTGCCTCTT
    CCCCCTTCTG CATTTGACTT TGTCATGTTA TTAGATATTT CAGATAATTC CTTGCTGGAC
    CGCATGAATT ACACAATGGC TGAAGCATTT TCAAGTGAAA CTTCTCATGA AGACATCACT
    CAACGTGTAA CTGCTGAAAA GCAAGATATG AATGAGGACC AGAATTTAAG AGACCAGATT
    CAGCACAGAA TTATAGGCTT CTTGGACAAC TGGCCTTTGT TGGAGCAATG GTTTACAGAG
    CCAGAAAATA TTTTGATAAA TATCAATGCT GAAGTAGATA AAGAGTCTTT ATGCAATAAA
    GTAAAAGAAA TGTTTATGAC TGAAATAATG AAAAAAGAGA ATAAAGTTAA AAAGAAATTA
    GAAGAAAAGG AAGCTGAGAA AAAAGAAGTT CCCCTGGGTG AGCCTCCGCC TCCTCCTCCT
    CCCCCTCCCC CTCCTCCTCC TGAGCCAGAA AAAGAGAAGG AAACCCATCC TCAACGAGAG
    CATTCAAAAA CTCCTACTGC AAAAGGGAAA CCACCAGCAG GTTCTCCTAA AGGAAAATCA
    CCAGGAGGGA AAGGACCACT AAAGAAATCA CCTGCTGATT CTACAGACAT GTCACCTGTT
    CCCATAGTGC CACCACCACC CAAGCCAGGA TCAGAAGAAT GGGTCTACGT GAATGAACCA
    ATTCCTGAGG AAATGCCTTC ATTTTTAGTG CCTTACTGGG AACTAATCGA AAATTCCTAC
    ATAAACTCCA TCAAAACAGT ACTCAGGCAT CTGAGAGAAG AACAGCATAG TGTGGTTGCT
    TACTTATACG AGGTTAGAAC AAATTTCCAG CAGTTTCTAT GGCGTCCAGA TCACAAGCAA
    GATTTTGTAT CTCAATGGCA GGCTGATTTC AATGCTCTTC CTGATGACCT GTGGGATGAT
    GAAGAAACAA AGTCTGAACT ACACCAAAGA GTAAATGATT TAAGAGACCG CCTGTGGGAC
    ATCTGTGATG CCCGGAAGGA AGAAGCAGAG CAGGAGCGTC AGGATATCAT TAACGAGAGC
    TGGTTGCAAG ACTCAATCGG AATAACGATG AACCATTTCT TCTCACTAAT GCAGACAGAA
    CTGAACCGTT TCCAAGATAC AAAGAGACTC CTTCAAGATT ATTACAGGGG AATGGAATGC
    AAAATTCCAA TCGATGACAG TAAGAGATTT ACTCGAATCC CGTTGGTCCA GCTGGATAGT
    AGTAGAGACA TTTTGGAAAG CCAACTCAGA ATTCCTCTAA TTCCACGAAT ATCCATTTCC
    CCGGATTCCG CTATGACCAA ACCAAAAACA AAGGCCATAC TAAAGGGCAA GATCGATTAC
    TCGCTGGAAA ATGTGGAGTT AAACTTTGAG GCAGATGAGA AGTTGGTGAT GGACACCTGG
    CAGCAGGCTT CTTTAGCAAT ATCTCACATG GTGGCTGCTG AAATTCATCA GAGGCTCATG
    GAAGAAGAAA AGGAAAACCA ACCAGCAGAC ACAAAAGAAA AATCTCCTCA GATGGGGACA
    AATAAAAAAG CCAAAGAGGA ACCACCCAAG AAAGAAAAGG CAGACAAAAA AGCAAAAGGC
    AGATCCCCGC CGACAGCAGA AGCCACGCCT GTGACAGTCA CAGCAGAGGA GATTACCCAA
    AAGGAGAAGA GAAACGAACT GATGCTCAAA ATCAAGGAAG AGCACCTCGC TGCCCTGCAG
    TTTGAAGAGA TAGCCACACA GTTCCGGCTT GAACTGATAA AGACAAAAGC ATTGGCTTTC
    CTTGAAGATT TACTAACAAA GGTGGTCGAT GTATATAAAC TCTTGGAAAA GTGGCTTGGT
    GAGAGGTACT TGAATGAAAT GGCCAGTGTG GAAAAATTAA CTGAGGTGGC CCGTTATCAC
    ATTGAAACAT CAACAAAAAT TCAGAATGAA CTTTACCTAG ACCAAGAAGA TTTCTTCATT
    AATGGAGATC TGAAAGTCTT TCCAGATCCC CCTCCCCCAG TGCGCCCCCC ACCTGTGGAA
    AAGGAAGAAA ATGGCACCCT GACCATCGAA CAGCTTGACA GTCTTAAAGA TCAGTTCTTA
    GATATTGCAC CCAAAGGCAG GGATCCTCCA GGTGACATGA ACCGAGTCAC TGAAAAGCAG
    AAAAGGCTGC AGAAGCTTGA CCATTACCTT CCAAAAAGGA ACGATCAATT TTTGAAAATC
    TGGCTGAAAA AATACCCTTG GCTTATATAT GATGAGATGT TAAATCTTAT GTTCTGTGGC
    CTGTGTCGTA AACATGGAGT GAAGTCAAAG GGAAATCAAG TGAGTTTTTT TTATGGCACT
    GACAACTTTA GGACAGAATT TCTCAATGCA CATCATCTCA GTGAGGCTCA TGCCAAGGCT
    TCACTAATGG AAGCAACAAA TGGTTCTCCA GTGAATGTCG CCACCACAGA GCTCATGGTG
    ACAACCATGA GCAAAATCAC CCTCGGGAGA GTAGAGAACT TATTCAGATC ATGCCATGCT
    ATCGCCAAGA CGGGACGTCC TCTCAAAGAC TTTATCTGGA TGTGCAAGTT GGATGATATG
    AAGGGGGTCG ATATCGGCCC CGTATTCAGA ACTAACAAAT CAGCAAGAAC CTTTACATAT
    TTTATTGCTG AAGTGGAACG GAAAAATCTT AGGGAGAAAC TAGAACAAAG TAAATTTTTC
    TCTGTCCTCA GTGACGGAAT CACAGACAGT TCAACCAAGG CGGCTGAACT TGTCTACGTG
    CAGTTTGCAC ACGCGGGAAA AGTGCATTGC CAAATTGCTG GGGTACAGAC AGTAGAAAGG
    GAGGATCCTT TGGCAATAAG AGATGCCATT GAAAGAACTC TAGAGAGAAA TCTTCAACTT
    AAGCTATCAG ACCAAGACTG GGCCAAGAAA CTGGTTGGTT TTGGAAGTGA TGGTGCCCTT
    GGCTCAGGGA GAGAAAACAG CAGGGTGGCC CTGCTTTTAA GAGAAATCCA GCCATGTGCT
    CTGACTGTAT ACTGCTTTGC ACACCATCTT GAACTGTCTT ACAAGGCGGT GTTCCAAAGC
    GTTCCCCTGT ACAACGACGT CAGAAATCTT CTTCACAGCA TCTACCACTT CTACCACAGT
    TCCCCTCTGC ACAGGAGTTC TCTGGTAACT GCTTTCAAAG GCCTTCACCT TCGACCTGTG
    ATGCCTTCTC AAGTAGGGGG CAGGAGGTGG CTTCAGGAAC TACAGGCAGC TCTCCAGAAC
    TTTCTCAAGG GATATCCTGC AATAGTCCAG CAACTGCATT CGGGAGATGA AGGCAGGAGT
    GACACCAGTC ATCAGAAAGC CAAGGAACTC CTGGGGTTCC TTCTCCAAGC AAACATTGTT
    AAATTTATCC ACTTCTTAGT GGATGTCATT AATGTCCTTA GCATTCTTTC CCGTGTCAAT
    CAAAACAGAA ATTCTTCCAT TGCTGATGTA TTTGCCACAT TAGAATCAAC ACTGGAAATG
    CTCAGAATAT ATCAAACAAG ACCAGGACCA AAGGAACGTT GGGTGGATTC GGTCACGCAC
    TTTCATGGCA ACTGCCTGGG AGGAAAGGGA GACATTTCTG ATGTGAGAAG AGTAGTCTTA
    ACACATCTTA TCAAGAGGCT GCAAGGTTGC TTCAGAGATG CCAGCCAAGA TGTGCTGAAG
    GCAACCATGA TTGGAAGCTT TAAACTGTGG CCTGCGAAGA TAAATCAAGA ATTTGGAGAA
    AAAGAGGTAT CCACCCTGAT TGCCCATTAT GAACCAGTAC TGGAAGCTGC CAACGTGGAA
    ATCGACGAAG TGGACGCTGA GTGGAGCATG CTGAAATTAG AGATTTATGC TAGATTTCAG
    AACATCCGCA AATTGACCTG GGATTTTGTG AATTCTGTTT ACTTACACAA GTATCCAAAT
    ATCTTGACGT TAGTGGACCT CGTGCTGACC CTCCCTGCAA GTTCCGCGGA GGCAGAACGT
    GGCTTTAGTC AGATGAAACT CACCAAGTCA CAAATGCGTG CCAAAATCAA GGCTGAAAGT
    ATGACGGATC TCCTTGTAAT TCAGTTGAAT TCTCCGGACA TTAATAACTT TGACCCTCGG
    AAAGCTATCC ATTTATGGAA CACAAGAACA CCATCTTCAG CTGATGACAC AAGACCTAAT
    TCTGCTTATT CATCAAACTC CGAAAGCCAT GATGAAAGCG ATTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36162
    Clone ID Related Accession (Same CDS sequence) XM_014113143.1 , XM_014113143.2
    Accession Version XM_014113143.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5295bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product sperm flagellar protein 2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGGAGA TTCTGTGCCA GTGGCTCAAC CAGGAGGTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAAT TCTGCACAAG
    TTTGAACTTC AGGGTGATTT TTCGGAATTT TCAGAAAGCA GAGTTTCAAA TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCGACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCACA ACATCATAAC GGAAAAGCCT GGGGCGGCAA CCAAACTCTT GTATCAGTTG
    TACATTGTTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAATAGAAAT GCAAACTATG
    CAACCCCTGG CAAACGTCAG ATACCAAAGT ATGAAAAGTG AGGCTTTTCG AGATCAACGC
    CTGAGCAGGA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCTG CATCAAATCG TACTTTGAGA GCACTGGAGG CCCAAAAAAT GATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAG ATTAAGAAGT TTGAAGCTTT AATAAAAAAG
    GATCTCCAGG CCAAAGAAAG TGCCTCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACT
    ACTGATTTGT TAAACACTTA TTCGGATGAT GACTACATTA AAAAAATCCA AAAGCGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT ATTAATGGAC
    CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCGTATCGGG AGGAGCAGCT GATTAACCGG
    CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATCTCTGTGC AGCTCATGCA TGTTCGGCAT
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCCGAGAAA AACAGTATGA GGAAAGACGA
    CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT
    GATTTTCAGG AACAAGCCAT TAGAGAGAAG GAAATTCATG AACAGATCAC TGTGGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATACA ATATGTGCAG AAATTTTGGA TCAAATACTT
    GATCTTTCCA CTAAAGTGTC AGACTACCGG ATGTTGACAA ATAATTTGAT TCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT AATGGAAAGC CCATATACAA GCAAGCCTCT
    ATTAAGCAAC TACCAGCCAC GCCCTCTGCA GAACAAGTTA TAGAACTGGA GAAGAGGGAC
    TTGCTGGATA GCAATGATTA TGAAGAATAT AAGAATATGG TTGGAGAGTG GGCTTTACCA
    GAAGAAATGG TTGATAATTT ACCACCCTCC AACAATACCA TATTGGGTCA TGTGATTCAC
    AGATTAATTG AAAAATCTCA TCCTCCTCAA GTCAAATCAA CAGTACTAGA ATTACCTTCA
    TTTGCTATTA AAGGGTGCTT AGTTGGAAAA ACATTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTACAAA AAGACTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT CCAAGAAGCA
    ATCAAAGCAT TCCATGACAA TGAAAAAATC AGTGAGGCTC TACCAATTGA GAAAGAAGCT
    GAAGAAAAGT CTCTACCAGT TCTGCAAGAG AAGAACAAAG AAAGCCAGGA TCTACAGAAT
    GCATTTTCAC CTCATCCAGT TTCAGAAGAA GCGTTACCAG AAACAGAAGA TAAAACTATA
    CTTAGTACTG ATACAAATAA AACTCCAAAA GCTGAAGAAG TCAAATCAAG TGATAGTTTC
    TCTGAACTGA CTGTGCGGGC TCAGCTTGGT GCAAAATCAG AAAAGATGCT GAAGAAAGGA
    AAGAGCATTT CTGACATGTT GCTAGTTAAT ATCTTGGTAA ATGCTATTAA TGAGGTACCT
    GTGGATCAAG GCTGGGTCTT GGATGGTTTT CCAATGACGT TAAACCAAGC AAAGCTTCTG
    GAGGAAGCTC TCACGGGCTA CAACAGAGAG GCCCTAGAAC TGGAAGAAAA AAAAACACAA
    ATACCCACAT TGGCTGTTGA TGCTAGCACC TCCAAAGAAG TGCCTCTTCC CCCTTCTGCA
    TTTGACTTTG TCATGTTATT AGATATTTCA GATAATTCCT TGCTGGACCG CATGAATTAC
    ACAATGGCTG AAGCATTTTC AAGTGAAACT TCTCATGAAG ACATCACTCA ACGTGTAACT
    GCTGAAAAGC AAGATATGAA TGAGGACCAG AATTTAAGAG ACCAGATTCA GCACAGAATT
    ATAGGCTTCT TGGACAACTG GCCTTTGTTG GAGCAATGGT TTACAGAGCC AGAAAATATT
    TTGATAAATA TCAATGCTGA AGTAGATAAA GAGTCTTTAT GCAATAAAGT AAAAGAAATG
    TTTATGACTG AAATAATGAA AAAAGAGAAT AAAGTTAAAA AGAAATTAGA AGAAAAGGAA
    GCTGAGAAAA AAGAAGTTCC CCTGGGTGAG CCTCCGCCTC CTCCTCCTCC CCCTCCCCCT
    CCTCCTCCTG AGCCAGAAAA AGAGAAGGAA ACCCATCCTC AACGAGAGCA TTCAAAAACT
    CCTACTGCAA AAGGGAAACC ACCAGCAGAA CCCTTGACTG GTAAGCCAGA CTCTCTTCAG
    GAAGGAAAAG GAAAGAAAGG GGACACTTCA CTCAAAAGAA AAGGTTCTCC TAAAGGAAAA
    TCACCAGGAG GGAAAGGACC ACTAAAGAAA TCACCTGCTG ATTCTACAGA CATGTCACCT
    GTTCCCATAG TGCCACCACC ACCCAAGCCA GGATCAGAAG AATGGGTCTA CGTGAATGAA
    CCAATTCCTG AGGAAATGCC TTCATTTTTA GTGCCTTACT GGGAACTAAT CGAAAATTCC
    TACATAAACT CCATCAAAAC AGTACTCAGG CATCTGAGAG AAGAACAGCA TAGTGTGGTT
    GCTTACTTAT ACGAGGTTAG AACAAATTTC CAGCAGTTTC TATGGCGTCC AGATCACAAG
    CAAGATTTTG TATCTCAATG GCAGGCTGAT TTCAATGCTC TTCCTGATGA CCTGTGGGAT
    GATGAAGAAA CAAAGTCTGA ACTACACCAA AGAGTAAATG ATTTAAGAGA CCGCCTGTGG
    GACATCTGTG ATGCCCGGAA GGAAGAAGCA GAGCAGGAGC GTCAGGATAT CATTAACGAG
    AGCTGGTTGC AAGACTCAAT CGGAATAACG ATGAACCATT TCTTCTCACT AATGCAGACA
    GAACTGAACC GTTTCCAAGA TACAAAGAGA CTCCTTCAAG ATTATTACAG GGGAATGGAA
    TGCAAAATTC CAATCGATGA CAGTAAGAGA TTTACTCGAA TCCCGTTGGT CCAGCTGGAT
    AGTAGTAGAG ACATTTTGGA AAGCCAACTC AGAATTCCTC TAATTCCACG AATATCCATT
    TCCCCGGATT CCGCTATGAC CAAACCAAAA ACAAAGGCCA TACTAAAGGG CAAGATCGAT
    TACTCGCTGG AAAATGTGGA GTTAAACTTT GAGGCAGATG AGAAGTTGGT GATGGACACC
    TGGCAGCAGG CTTCTTTAGC AATATCTCAC ATGGTGGCTG CTGAAATTCA TCAGAGGCTC
    ATGGAAGAAG AAAAGGAAAA CCAACCAGCA GACACAAAAG AAAAATCTCC TCAGATGGGG
    ACAAATAAAA AAGCCAAAGA GGAACCACCC AAGAAAGAAA AGGCAGACAA AAAAGCAAAA
    GGCAGATCCC CGCCGACAGC AGAAGCCACG CCTGTGACAG TCACAGCAGA GGAGATTACC
    CAAAAGGAGA AGAGAAACGA ACTGATGCTC AAAATCAAGG AAGAGCACCT CGCTGCCCTG
    CAGTTTGAAG AGATAGCCAC ACAGTTCCGG CTTGAACTGA TAAAGACAAA AGCATTGGCT
    TTCCTTGAAG ATTTACTAAC AAAGGTGGTC GATGTATATA AACTCTTGGA AAAGTGGCTT
    GGTGAGAGGT ACTTGAATGA AATGGCCAGT GTGGAAAAAT TAACTGAGGT GGCCCGTTAT
    CACATTGAAA CATCAACAAA AATTCAGAAT GAACTTTACC TAGACCAAGA AGATTTCTTC
    ATTAATGGAG ATCTGAAAGT CTTTCCAGAT CCCCCTCCCC CAGTGCGCCC CCCACCTGTG
    GAAAAGGAAG AAAATGGCAC CCTGACCATC GAACAGCTTG ACAGTCTTAA AGATCAGTTC
    TTAGATATTG CACCCAAAGG CATAATAGGA CATAAAGCAT TTACTGACAT TCTGCTGGAT
    CTGGTAACCC TGAACCTTGG AACAAACAAC TTACCTAGTA GTTGGATGCA CCTCACGCAA
    CCAGAATTGC AGGAGTTAAC ATCTTTAGTA ATAGTAAATT CGGAGTTTGT GGACTGGCGG
    AAATTCCTAT TAGTAATCTC GATGCCTTGG CCCATCCCCA TGGAGGAAGA GCTCCTCGAA
    ACCCTGCAGA GGTTCAAGGC GGTGGATGAA GAGCAGTTGG GCACCATCAC TTTTGAGCAG
    TATATGCAGG CTGGTCTGTG GTTTACAGGA GATGAATATA TAAAAATTCC AGAAAATCCT
    CTTGAACCTC TGCCGTTCAA TAGGCAGGAG CATCTTATAG AGTTTTTCTT TAGGCTATTT
    GCTGACCATG AGAAGGATCC ACCTCAACTT GACTACACAC AGATGCTGCT CTATTTTGCT
    TGCCATCCAG ATGCTATGGA AGGGGTCTAC AGGGCCCTCA GTGTGGCCAT AGGAACTCAT
    GTCTTCCAAC CAGTCGAAAC TATTGCTATT ATTGGAGAAA AGTCCTCCTC CTTGACTGAT
    GCAAGTCCAG TTGAGGAGTA TCCTGAACTC GAGGGAAACT TTATGCATGA GGAAGGAGAA
    TTAAAAGAAG ACAGGGAGGA AAAGGAAGTA GAAATCCCTG AGAATGCAAA CACTGAAAAG
    GTATCCCTGG AAAGACTACT CAAAGTGTTC CAAGGAGGAA AAGAAGTACA GGATGCTAAC
    AGATTCACCA GCCACCTACA GATAGAGAAC ACTTACTCAG AGAACTTCGT AAAAGTATTT
    CAAGACCTAG GTGCCAAGGA TCTGGAGCCA GTTGAAGTTG CTGTTCTCTT GAAGCATCCT
    TTTATTCAAG ACCTGATTTC AAATTATTCA GACTATAAAA TCCCTGATAT TAAAATAATT
    CTCCAAAGGA GTGAGCATGT ACAAGGAAGT GATGGAGAAA GATCTCCTTC AAGACTTACA
    GAGGAAAAGA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968618.1
    CDS159..5453
    Translation

    Target ORF information:

    RefSeq Version XM_014113143.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113143.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGGAGA TTCTGTGCCA GTGGCTCAAC CAGGAGGTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAAT TCTGCACAAG
    TTTGAACTTC AGGGTGATTT TTCGGAATTT TCAGAAAGCA GAGTTTCAAA TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCGACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCACA ACATCATAAC GGAAAAGCCT GGGGCGGCAA CCAAACTCTT GTATCAGTTG
    TACATTGTTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAATAGAAAT GCAAACTATG
    CAACCCCTGG CAAACGTCAG ATACCAAAGT ATGAAAAGTG AGGCTTTTCG AGATCAACGC
    CTGAGCAGGA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCTG CATCAAATCG TACTTTGAGA GCACTGGAGG CCCAAAAAAT GATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAG ATTAAGAAGT TTGAAGCTTT AATAAAAAAG
    GATCTCCAGG CCAAAGAAAG TGCCTCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACT
    ACTGATTTGT TAAACACTTA TTCGGATGAT GACTACATTA AAAAAATCCA AAAGCGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT ATTAATGGAC
    CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCGTATCGGG AGGAGCAGCT GATTAACCGG
    CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATCTCTGTGC AGCTCATGCA TGTTCGGCAT
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCCGAGAAA AACAGTATGA GGAAAGACGA
    CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT
    GATTTTCAGG AACAAGCCAT TAGAGAGAAG GAAATTCATG AACAGATCAC TGTGGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATACA ATATGTGCAG AAATTTTGGA TCAAATACTT
    GATCTTTCCA CTAAAGTGTC AGACTACCGG ATGTTGACAA ATAATTTGAT TCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT AATGGAAAGC CCATATACAA GCAAGCCTCT
    ATTAAGCAAC TACCAGCCAC GCCCTCTGCA GAACAAGTTA TAGAACTGGA GAAGAGGGAC
    TTGCTGGATA GCAATGATTA TGAAGAATAT AAGAATATGG TTGGAGAGTG GGCTTTACCA
    GAAGAAATGG TTGATAATTT ACCACCCTCC AACAATACCA TATTGGGTCA TGTGATTCAC
    AGATTAATTG AAAAATCTCA TCCTCCTCAA GTCAAATCAA CAGTACTAGA ATTACCTTCA
    TTTGCTATTA AAGGGTGCTT AGTTGGAAAA ACATTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTACAAA AAGACTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT CCAAGAAGCA
    ATCAAAGCAT TCCATGACAA TGAAAAAATC AGTGAGGCTC TACCAATTGA GAAAGAAGCT
    GAAGAAAAGT CTCTACCAGT TCTGCAAGAG AAGAACAAAG AAAGCCAGGA TCTACAGAAT
    GCATTTTCAC CTCATCCAGT TTCAGAAGAA GCGTTACCAG AAACAGAAGA TAAAACTATA
    CTTAGTACTG ATACAAATAA AACTCCAAAA GCTGAAGAAG TCAAATCAAG TGATAGTTTC
    TCTGAACTGA CTGTGCGGGC TCAGCTTGGT GCAAAATCAG AAAAGATGCT GAAGAAAGGA
    AAGAGCATTT CTGACATGTT GCTAGTTAAT ATCTTGGTAA ATGCTATTAA TGAGGTACCT
    GTGGATCAAG GCTGGGTCTT GGATGGTTTT CCAATGACGT TAAACCAAGC AAAGCTTCTG
    GAGGAAGCTC TCACGGGCTA CAACAGAGAG GCCCTAGAAC TGGAAGAAAA AAAAACACAA
    ATACCCACAT TGGCTGTTGA TGCTAGCACC TCCAAAGAAG TGCCTCTTCC CCCTTCTGCA
    TTTGACTTTG TCATGTTATT AGATATTTCA GATAATTCCT TGCTGGACCG CATGAATTAC
    ACAATGGCTG AAGCATTTTC AAGTGAAACT TCTCATGAAG ACATCACTCA ACGTGTAACT
    GCTGAAAAGC AAGATATGAA TGAGGACCAG AATTTAAGAG ACCAGATTCA GCACAGAATT
    ATAGGCTTCT TGGACAACTG GCCTTTGTTG GAGCAATGGT TTACAGAGCC AGAAAATATT
    TTGATAAATA TCAATGCTGA AGTAGATAAA GAGTCTTTAT GCAATAAAGT AAAAGAAATG
    TTTATGACTG AAATAATGAA AAAAGAGAAT AAAGTTAAAA AGAAATTAGA AGAAAAGGAA
    GCTGAGAAAA AAGAAGTTCC CCTGGGTGAG CCTCCGCCTC CTCCTCCTCC CCCTCCCCCT
    CCTCCTCCTG AGCCAGAAAA AGAGAAGGAA ACCCATCCTC AACGAGAGCA TTCAAAAACT
    CCTACTGCAA AAGGGAAACC ACCAGCAGAA CCCTTGACTG GTAAGCCAGA CTCTCTTCAG
    GAAGGAAAAG GAAAGAAAGG GGACACTTCA CTCAAAAGAA AAGGTTCTCC TAAAGGAAAA
    TCACCAGGAG GGAAAGGACC ACTAAAGAAA TCACCTGCTG ATTCTACAGA CATGTCACCT
    GTTCCCATAG TGCCACCACC ACCCAAGCCA GGATCAGAAG AATGGGTCTA CGTGAATGAA
    CCAATTCCTG AGGAAATGCC TTCATTTTTA GTGCCTTACT GGGAACTAAT CGAAAATTCC
    TACATAAACT CCATCAAAAC AGTACTCAGG CATCTGAGAG AAGAACAGCA TAGTGTGGTT
    GCTTACTTAT ACGAGGTTAG AACAAATTTC CAGCAGTTTC TATGGCGTCC AGATCACAAG
    CAAGATTTTG TATCTCAATG GCAGGCTGAT TTCAATGCTC TTCCTGATGA CCTGTGGGAT
    GATGAAGAAA CAAAGTCTGA ACTACACCAA AGAGTAAATG ATTTAAGAGA CCGCCTGTGG
    GACATCTGTG ATGCCCGGAA GGAAGAAGCA GAGCAGGAGC GTCAGGATAT CATTAACGAG
    AGCTGGTTGC AAGACTCAAT CGGAATAACG ATGAACCATT TCTTCTCACT AATGCAGACA
    GAACTGAACC GTTTCCAAGA TACAAAGAGA CTCCTTCAAG ATTATTACAG GGGAATGGAA
    TGCAAAATTC CAATCGATGA CAGTAAGAGA TTTACTCGAA TCCCGTTGGT CCAGCTGGAT
    AGTAGTAGAG ACATTTTGGA AAGCCAACTC AGAATTCCTC TAATTCCACG AATATCCATT
    TCCCCGGATT CCGCTATGAC CAAACCAAAA ACAAAGGCCA TACTAAAGGG CAAGATCGAT
    TACTCGCTGG AAAATGTGGA GTTAAACTTT GAGGCAGATG AGAAGTTGGT GATGGACACC
    TGGCAGCAGG CTTCTTTAGC AATATCTCAC ATGGTGGCTG CTGAAATTCA TCAGAGGCTC
    ATGGAAGAAG AAAAGGAAAA CCAACCAGCA GACACAAAAG AAAAATCTCC TCAGATGGGG
    ACAAATAAAA AAGCCAAAGA GGAACCACCC AAGAAAGAAA AGGCAGACAA AAAAGCAAAA
    GGCAGATCCC CGCCGACAGC AGAAGCCACG CCTGTGACAG TCACAGCAGA GGAGATTACC
    CAAAAGGAGA AGAGAAACGA ACTGATGCTC AAAATCAAGG AAGAGCACCT CGCTGCCCTG
    CAGTTTGAAG AGATAGCCAC ACAGTTCCGG CTTGAACTGA TAAAGACAAA AGCATTGGCT
    TTCCTTGAAG ATTTACTAAC AAAGGTGGTC GATGTATATA AACTCTTGGA AAAGTGGCTT
    GGTGAGAGGT ACTTGAATGA AATGGCCAGT GTGGAAAAAT TAACTGAGGT GGCCCGTTAT
    CACATTGAAA CATCAACAAA AATTCAGAAT GAACTTTACC TAGACCAAGA AGATTTCTTC
    ATTAATGGAG ATCTGAAAGT CTTTCCAGAT CCCCCTCCCC CAGTGCGCCC CCCACCTGTG
    GAAAAGGAAG AAAATGGCAC CCTGACCATC GAACAGCTTG ACAGTCTTAA AGATCAGTTC
    TTAGATATTG CACCCAAAGG CATAATAGGA CATAAAGCAT TTACTGACAT TCTGCTGGAT
    CTGGTAACCC TGAACCTTGG AACAAACAAC TTACCTAGTA GTTGGATGCA CCTCACGCAA
    CCAGAATTGC AGGAGTTAAC ATCTTTAGTA ATAGTAAATT CGGAGTTTGT GGACTGGCGG
    AAATTCCTAT TAGTAATCTC GATGCCTTGG CCCATCCCCA TGGAGGAAGA GCTCCTCGAA
    ACCCTGCAGA GGTTCAAGGC GGTGGATGAA GAGCAGTTGG GCACCATCAC TTTTGAGCAG
    TATATGCAGG CTGGTCTGTG GTTTACAGGA GATGAATATA TAAAAATTCC AGAAAATCCT
    CTTGAACCTC TGCCGTTCAA TAGGCAGGAG CATCTTATAG AGTTTTTCTT TAGGCTATTT
    GCTGACCATG AGAAGGATCC ACCTCAACTT GACTACACAC AGATGCTGCT CTATTTTGCT
    TGCCATCCAG ATGCTATGGA AGGGGTCTAC AGGGCCCTCA GTGTGGCCAT AGGAACTCAT
    GTCTTCCAAC CAGTCGAAAC TATTGCTATT ATTGGAGAAA AGTCCTCCTC CTTGACTGAT
    GCAAGTCCAG TTGAGGAGTA TCCTGAACTC GAGGGAAACT TTATGCATGA GGAAGGAGAA
    TTAAAAGAAG ACAGGGAGGA AAAGGAAGTA GAAATCCCTG AGAATGCAAA CACTGAAAAG
    GTATCCCTGG AAAGACTACT CAAAGTGTTC CAAGGAGGAA AAGAAGTACA GGATGCTAAC
    AGATTCACCA GCCACCTACA GATAGAGAAC ACTTACTCAG AGAACTTCGT AAAAGTATTT
    CAAGACCTAG GTGCCAAGGA TCTGGAGCCA GTTGAAGTTG CTGTTCTCTT GAAGCATCCT
    TTTATTCAAG ACCTGATTTC AAATTATTCA GACTATAAAA TCCCTGATAT TAAAATAATT
    CTCCAAAGGA GTGAGCATGT ACAAGGAAGT GATGGAGAAA GATCTCCTTC AAGACTTACA
    GAGGAAAAGA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36162
    Clone ID Related Accession (Same CDS sequence) XM_014113143.1 , XM_014113143.2
    Accession Version XM_014113143.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5295bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product sperm flagellar protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113143.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGGAGA TTCTGTGCCA GTGGCTCAAC CAGGAGGTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAAT TCTGCACAAG
    TTTGAACTTC AGGGTGATTT TTCGGAATTT TCAGAAAGCA GAGTTTCAAA TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCGACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCACA ACATCATAAC GGAAAAGCCT GGGGCGGCAA CCAAACTCTT GTATCAGTTG
    TACATTGTTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAATAGAAAT GCAAACTATG
    CAACCCCTGG CAAACGTCAG ATACCAAAGT ATGAAAAGTG AGGCTTTTCG AGATCAACGC
    CTGAGCAGGA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCTG CATCAAATCG TACTTTGAGA GCACTGGAGG CCCAAAAAAT GATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAG ATTAAGAAGT TTGAAGCTTT AATAAAAAAG
    GATCTCCAGG CCAAAGAAAG TGCCTCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACT
    ACTGATTTGT TAAACACTTA TTCGGATGAT GACTACATTA AAAAAATCCA AAAGCGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT ATTAATGGAC
    CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCGTATCGGG AGGAGCAGCT GATTAACCGG
    CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATCTCTGTGC AGCTCATGCA TGTTCGGCAT
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCCGAGAAA AACAGTATGA GGAAAGACGA
    CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT
    GATTTTCAGG AACAAGCCAT TAGAGAGAAG GAAATTCATG AACAGATCAC TGTGGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATACA ATATGTGCAG AAATTTTGGA TCAAATACTT
    GATCTTTCCA CTAAAGTGTC AGACTACCGG ATGTTGACAA ATAATTTGAT TCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT AATGGAAAGC CCATATACAA GCAAGCCTCT
    ATTAAGCAAC TACCAGCCAC GCCCTCTGCA GAACAAGTTA TAGAACTGGA GAAGAGGGAC
    TTGCTGGATA GCAATGATTA TGAAGAATAT AAGAATATGG TTGGAGAGTG GGCTTTACCA
    GAAGAAATGG TTGATAATTT ACCACCCTCC AACAATACCA TATTGGGTCA TGTGATTCAC
    AGATTAATTG AAAAATCTCA TCCTCCTCAA GTCAAATCAA CAGTACTAGA ATTACCTTCA
    TTTGCTATTA AAGGGTGCTT AGTTGGAAAA ACATTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTACAAA AAGACTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT CCAAGAAGCA
    ATCAAAGCAT TCCATGACAA TGAAAAAATC AGTGAGGCTC TACCAATTGA GAAAGAAGCT
    GAAGAAAAGT CTCTACCAGT TCTGCAAGAG AAGAACAAAG AAAGCCAGGA TCTACAGAAT
    GCATTTTCAC CTCATCCAGT TTCAGAAGAA GCGTTACCAG AAACAGAAGA TAAAACTATA
    CTTAGTACTG ATACAAATAA AACTCCAAAA GCTGAAGAAG TCAAATCAAG TGATAGTTTC
    TCTGAACTGA CTGTGCGGGC TCAGCTTGGT GCAAAATCAG AAAAGATGCT GAAGAAAGGA
    AAGAGCATTT CTGACATGTT GCTAGTTAAT ATCTTGGTAA ATGCTATTAA TGAGGTACCT
    GTGGATCAAG GCTGGGTCTT GGATGGTTTT CCAATGACGT TAAACCAAGC AAAGCTTCTG
    GAGGAAGCTC TCACGGGCTA CAACAGAGAG GCCCTAGAAC TGGAAGAAAA AAAAACACAA
    ATACCCACAT TGGCTGTTGA TGCTAGCACC TCCAAAGAAG TGCCTCTTCC CCCTTCTGCA
    TTTGACTTTG TCATGTTATT AGATATTTCA GATAATTCCT TGCTGGACCG CATGAATTAC
    ACAATGGCTG AAGCATTTTC AAGTGAAACT TCTCATGAAG ACATCACTCA ACGTGTAACT
    GCTGAAAAGC AAGATATGAA TGAGGACCAG AATTTAAGAG ACCAGATTCA GCACAGAATT
    ATAGGCTTCT TGGACAACTG GCCTTTGTTG GAGCAATGGT TTACAGAGCC AGAAAATATT
    TTGATAAATA TCAATGCTGA AGTAGATAAA GAGTCTTTAT GCAATAAAGT AAAAGAAATG
    TTTATGACTG AAATAATGAA AAAAGAGAAT AAAGTTAAAA AGAAATTAGA AGAAAAGGAA
    GCTGAGAAAA AAGAAGTTCC CCTGGGTGAG CCTCCGCCTC CTCCTCCTCC CCCTCCCCCT
    CCTCCTCCTG AGCCAGAAAA AGAGAAGGAA ACCCATCCTC AACGAGAGCA TTCAAAAACT
    CCTACTGCAA AAGGGAAACC ACCAGCAGAA CCCTTGACTG GTAAGCCAGA CTCTCTTCAG
    GAAGGAAAAG GAAAGAAAGG GGACACTTCA CTCAAAAGAA AAGGTTCTCC TAAAGGAAAA
    TCACCAGGAG GGAAAGGACC ACTAAAGAAA TCACCTGCTG ATTCTACAGA CATGTCACCT
    GTTCCCATAG TGCCACCACC ACCCAAGCCA GGATCAGAAG AATGGGTCTA CGTGAATGAA
    CCAATTCCTG AGGAAATGCC TTCATTTTTA GTGCCTTACT GGGAACTAAT CGAAAATTCC
    TACATAAACT CCATCAAAAC AGTACTCAGG CATCTGAGAG AAGAACAGCA TAGTGTGGTT
    GCTTACTTAT ACGAGGTTAG AACAAATTTC CAGCAGTTTC TATGGCGTCC AGATCACAAG
    CAAGATTTTG TATCTCAATG GCAGGCTGAT TTCAATGCTC TTCCTGATGA CCTGTGGGAT
    GATGAAGAAA CAAAGTCTGA ACTACACCAA AGAGTAAATG ATTTAAGAGA CCGCCTGTGG
    GACATCTGTG ATGCCCGGAA GGAAGAAGCA GAGCAGGAGC GTCAGGATAT CATTAACGAG
    AGCTGGTTGC AAGACTCAAT CGGAATAACG ATGAACCATT TCTTCTCACT AATGCAGACA
    GAACTGAACC GTTTCCAAGA TACAAAGAGA CTCCTTCAAG ATTATTACAG GGGAATGGAA
    TGCAAAATTC CAATCGATGA CAGTAAGAGA TTTACTCGAA TCCCGTTGGT CCAGCTGGAT
    AGTAGTAGAG ACATTTTGGA AAGCCAACTC AGAATTCCTC TAATTCCACG AATATCCATT
    TCCCCGGATT CCGCTATGAC CAAACCAAAA ACAAAGGCCA TACTAAAGGG CAAGATCGAT
    TACTCGCTGG AAAATGTGGA GTTAAACTTT GAGGCAGATG AGAAGTTGGT GATGGACACC
    TGGCAGCAGG CTTCTTTAGC AATATCTCAC ATGGTGGCTG CTGAAATTCA TCAGAGGCTC
    ATGGAAGAAG AAAAGGAAAA CCAACCAGCA GACACAAAAG AAAAATCTCC TCAGATGGGG
    ACAAATAAAA AAGCCAAAGA GGAACCACCC AAGAAAGAAA AGGCAGACAA AAAAGCAAAA
    GGCAGATCCC CGCCGACAGC AGAAGCCACG CCTGTGACAG TCACAGCAGA GGAGATTACC
    CAAAAGGAGA AGAGAAACGA ACTGATGCTC AAAATCAAGG AAGAGCACCT CGCTGCCCTG
    CAGTTTGAAG AGATAGCCAC ACAGTTCCGG CTTGAACTGA TAAAGACAAA AGCATTGGCT
    TTCCTTGAAG ATTTACTAAC AAAGGTGGTC GATGTATATA AACTCTTGGA AAAGTGGCTT
    GGTGAGAGGT ACTTGAATGA AATGGCCAGT GTGGAAAAAT TAACTGAGGT GGCCCGTTAT
    CACATTGAAA CATCAACAAA AATTCAGAAT GAACTTTACC TAGACCAAGA AGATTTCTTC
    ATTAATGGAG ATCTGAAAGT CTTTCCAGAT CCCCCTCCCC CAGTGCGCCC CCCACCTGTG
    GAAAAGGAAG AAAATGGCAC CCTGACCATC GAACAGCTTG ACAGTCTTAA AGATCAGTTC
    TTAGATATTG CACCCAAAGG CATAATAGGA CATAAAGCAT TTACTGACAT TCTGCTGGAT
    CTGGTAACCC TGAACCTTGG AACAAACAAC TTACCTAGTA GTTGGATGCA CCTCACGCAA
    CCAGAATTGC AGGAGTTAAC ATCTTTAGTA ATAGTAAATT CGGAGTTTGT GGACTGGCGG
    AAATTCCTAT TAGTAATCTC GATGCCTTGG CCCATCCCCA TGGAGGAAGA GCTCCTCGAA
    ACCCTGCAGA GGTTCAAGGC GGTGGATGAA GAGCAGTTGG GCACCATCAC TTTTGAGCAG
    TATATGCAGG CTGGTCTGTG GTTTACAGGA GATGAATATA TAAAAATTCC AGAAAATCCT
    CTTGAACCTC TGCCGTTCAA TAGGCAGGAG CATCTTATAG AGTTTTTCTT TAGGCTATTT
    GCTGACCATG AGAAGGATCC ACCTCAACTT GACTACACAC AGATGCTGCT CTATTTTGCT
    TGCCATCCAG ATGCTATGGA AGGGGTCTAC AGGGCCCTCA GTGTGGCCAT AGGAACTCAT
    GTCTTCCAAC CAGTCGAAAC TATTGCTATT ATTGGAGAAA AGTCCTCCTC CTTGACTGAT
    GCAAGTCCAG TTGAGGAGTA TCCTGAACTC GAGGGAAACT TTATGCATGA GGAAGGAGAA
    TTAAAAGAAG ACAGGGAGGA AAAGGAAGTA GAAATCCCTG AGAATGCAAA CACTGAAAAG
    GTATCCCTGG AAAGACTACT CAAAGTGTTC CAAGGAGGAA AAGAAGTACA GGATGCTAAC
    AGATTCACCA GCCACCTACA GATAGAGAAC ACTTACTCAG AGAACTTCGT AAAAGTATTT
    CAAGACCTAG GTGCCAAGGA TCTGGAGCCA GTTGAAGTTG CTGTTCTCTT GAAGCATCCT
    TTTATTCAAG ACCTGATTTC AAATTATTCA GACTATAAAA TCCCTGATAT TAAAATAATT
    CTCCAAAGGA GTGAGCATGT ACAAGGAAGT GATGGAGAAA GATCTCCTTC AAGACTTACA
    GAGGAAAAGA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968618.1
    CDS159..5453
    Translation

    Target ORF information:

    RefSeq Version XM_014113143.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris sperm flagellar protein 2 (LOC489235), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113143.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGGAGA TTCTGTGCCA GTGGCTCAAC CAGGAGGTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAAT TCTGCACAAG
    TTTGAACTTC AGGGTGATTT TTCGGAATTT TCAGAAAGCA GAGTTTCAAA TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCGACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCACA ACATCATAAC GGAAAAGCCT GGGGCGGCAA CCAAACTCTT GTATCAGTTG
    TACATTGTTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAATAGAAAT GCAAACTATG
    CAACCCCTGG CAAACGTCAG ATACCAAAGT ATGAAAAGTG AGGCTTTTCG AGATCAACGC
    CTGAGCAGGA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCTG CATCAAATCG TACTTTGAGA GCACTGGAGG CCCAAAAAAT GATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAG ATTAAGAAGT TTGAAGCTTT AATAAAAAAG
    GATCTCCAGG CCAAAGAAAG TGCCTCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACT
    ACTGATTTGT TAAACACTTA TTCGGATGAT GACTACATTA AAAAAATCCA AAAGCGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT ATTAATGGAC
    CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCGTATCGGG AGGAGCAGCT GATTAACCGG
    CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATCTCTGTGC AGCTCATGCA TGTTCGGCAT
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCCGAGAAA AACAGTATGA GGAAAGACGA
    CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT
    GATTTTCAGG AACAAGCCAT TAGAGAGAAG GAAATTCATG AACAGATCAC TGTGGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATACA ATATGTGCAG AAATTTTGGA TCAAATACTT
    GATCTTTCCA CTAAAGTGTC AGACTACCGG ATGTTGACAA ATAATTTGAT TCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT AATGGAAAGC CCATATACAA GCAAGCCTCT
    ATTAAGCAAC TACCAGCCAC GCCCTCTGCA GAACAAGTTA TAGAACTGGA GAAGAGGGAC
    TTGCTGGATA GCAATGATTA TGAAGAATAT AAGAATATGG TTGGAGAGTG GGCTTTACCA
    GAAGAAATGG TTGATAATTT ACCACCCTCC AACAATACCA TATTGGGTCA TGTGATTCAC
    AGATTAATTG AAAAATCTCA TCCTCCTCAA GTCAAATCAA CAGTACTAGA ATTACCTTCA
    TTTGCTATTA AAGGGTGCTT AGTTGGAAAA ACATTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTACAAA AAGACTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT CCAAGAAGCA
    ATCAAAGCAT TCCATGACAA TGAAAAAATC AGTGAGGCTC TACCAATTGA GAAAGAAGCT
    GAAGAAAAGT CTCTACCAGT TCTGCAAGAG AAGAACAAAG AAAGCCAGGA TCTACAGAAT
    GCATTTTCAC CTCATCCAGT TTCAGAAGAA GCGTTACCAG AAACAGAAGA TAAAACTATA
    CTTAGTACTG ATACAAATAA AACTCCAAAA GCTGAAGAAG TCAAATCAAG TGATAGTTTC
    TCTGAACTGA CTGTGCGGGC TCAGCTTGGT GCAAAATCAG AAAAGATGCT GAAGAAAGGA
    AAGAGCATTT CTGACATGTT GCTAGTTAAT ATCTTGGTAA ATGCTATTAA TGAGGTACCT
    GTGGATCAAG GCTGGGTCTT GGATGGTTTT CCAATGACGT TAAACCAAGC AAAGCTTCTG
    GAGGAAGCTC TCACGGGCTA CAACAGAGAG GCCCTAGAAC TGGAAGAAAA AAAAACACAA
    ATACCCACAT TGGCTGTTGA TGCTAGCACC TCCAAAGAAG TGCCTCTTCC CCCTTCTGCA
    TTTGACTTTG TCATGTTATT AGATATTTCA GATAATTCCT TGCTGGACCG CATGAATTAC
    ACAATGGCTG AAGCATTTTC AAGTGAAACT TCTCATGAAG ACATCACTCA ACGTGTAACT
    GCTGAAAAGC AAGATATGAA TGAGGACCAG AATTTAAGAG ACCAGATTCA GCACAGAATT
    ATAGGCTTCT TGGACAACTG GCCTTTGTTG GAGCAATGGT TTACAGAGCC AGAAAATATT
    TTGATAAATA TCAATGCTGA AGTAGATAAA GAGTCTTTAT GCAATAAAGT AAAAGAAATG
    TTTATGACTG AAATAATGAA AAAAGAGAAT AAAGTTAAAA AGAAATTAGA AGAAAAGGAA
    GCTGAGAAAA AAGAAGTTCC CCTGGGTGAG CCTCCGCCTC CTCCTCCTCC CCCTCCCCCT
    CCTCCTCCTG AGCCAGAAAA AGAGAAGGAA ACCCATCCTC AACGAGAGCA TTCAAAAACT
    CCTACTGCAA AAGGGAAACC ACCAGCAGAA CCCTTGACTG GTAAGCCAGA CTCTCTTCAG
    GAAGGAAAAG GAAAGAAAGG GGACACTTCA CTCAAAAGAA AAGGTTCTCC TAAAGGAAAA
    TCACCAGGAG GGAAAGGACC ACTAAAGAAA TCACCTGCTG ATTCTACAGA CATGTCACCT
    GTTCCCATAG TGCCACCACC ACCCAAGCCA GGATCAGAAG AATGGGTCTA CGTGAATGAA
    CCAATTCCTG AGGAAATGCC TTCATTTTTA GTGCCTTACT GGGAACTAAT CGAAAATTCC
    TACATAAACT CCATCAAAAC AGTACTCAGG CATCTGAGAG AAGAACAGCA TAGTGTGGTT
    GCTTACTTAT ACGAGGTTAG AACAAATTTC CAGCAGTTTC TATGGCGTCC AGATCACAAG
    CAAGATTTTG TATCTCAATG GCAGGCTGAT TTCAATGCTC TTCCTGATGA CCTGTGGGAT
    GATGAAGAAA CAAAGTCTGA ACTACACCAA AGAGTAAATG ATTTAAGAGA CCGCCTGTGG
    GACATCTGTG ATGCCCGGAA GGAAGAAGCA GAGCAGGAGC GTCAGGATAT CATTAACGAG
    AGCTGGTTGC AAGACTCAAT CGGAATAACG ATGAACCATT TCTTCTCACT AATGCAGACA
    GAACTGAACC GTTTCCAAGA TACAAAGAGA CTCCTTCAAG ATTATTACAG GGGAATGGAA
    TGCAAAATTC CAATCGATGA CAGTAAGAGA TTTACTCGAA TCCCGTTGGT CCAGCTGGAT
    AGTAGTAGAG ACATTTTGGA AAGCCAACTC AGAATTCCTC TAATTCCACG AATATCCATT
    TCCCCGGATT CCGCTATGAC CAAACCAAAA ACAAAGGCCA TACTAAAGGG CAAGATCGAT
    TACTCGCTGG AAAATGTGGA GTTAAACTTT GAGGCAGATG AGAAGTTGGT GATGGACACC
    TGGCAGCAGG CTTCTTTAGC AATATCTCAC ATGGTGGCTG CTGAAATTCA TCAGAGGCTC
    ATGGAAGAAG AAAAGGAAAA CCAACCAGCA GACACAAAAG AAAAATCTCC TCAGATGGGG
    ACAAATAAAA AAGCCAAAGA GGAACCACCC AAGAAAGAAA AGGCAGACAA AAAAGCAAAA
    GGCAGATCCC CGCCGACAGC AGAAGCCACG CCTGTGACAG TCACAGCAGA GGAGATTACC
    CAAAAGGAGA AGAGAAACGA ACTGATGCTC AAAATCAAGG AAGAGCACCT CGCTGCCCTG
    CAGTTTGAAG AGATAGCCAC ACAGTTCCGG CTTGAACTGA TAAAGACAAA AGCATTGGCT
    TTCCTTGAAG ATTTACTAAC AAAGGTGGTC GATGTATATA AACTCTTGGA AAAGTGGCTT
    GGTGAGAGGT ACTTGAATGA AATGGCCAGT GTGGAAAAAT TAACTGAGGT GGCCCGTTAT
    CACATTGAAA CATCAACAAA AATTCAGAAT GAACTTTACC TAGACCAAGA AGATTTCTTC
    ATTAATGGAG ATCTGAAAGT CTTTCCAGAT CCCCCTCCCC CAGTGCGCCC CCCACCTGTG
    GAAAAGGAAG AAAATGGCAC CCTGACCATC GAACAGCTTG ACAGTCTTAA AGATCAGTTC
    TTAGATATTG CACCCAAAGG CATAATAGGA CATAAAGCAT TTACTGACAT TCTGCTGGAT
    CTGGTAACCC TGAACCTTGG AACAAACAAC TTACCTAGTA GTTGGATGCA CCTCACGCAA
    CCAGAATTGC AGGAGTTAAC ATCTTTAGTA ATAGTAAATT CGGAGTTTGT GGACTGGCGG
    AAATTCCTAT TAGTAATCTC GATGCCTTGG CCCATCCCCA TGGAGGAAGA GCTCCTCGAA
    ACCCTGCAGA GGTTCAAGGC GGTGGATGAA GAGCAGTTGG GCACCATCAC TTTTGAGCAG
    TATATGCAGG CTGGTCTGTG GTTTACAGGA GATGAATATA TAAAAATTCC AGAAAATCCT
    CTTGAACCTC TGCCGTTCAA TAGGCAGGAG CATCTTATAG AGTTTTTCTT TAGGCTATTT
    GCTGACCATG AGAAGGATCC ACCTCAACTT GACTACACAC AGATGCTGCT CTATTTTGCT
    TGCCATCCAG ATGCTATGGA AGGGGTCTAC AGGGCCCTCA GTGTGGCCAT AGGAACTCAT
    GTCTTCCAAC CAGTCGAAAC TATTGCTATT ATTGGAGAAA AGTCCTCCTC CTTGACTGAT
    GCAAGTCCAG TTGAGGAGTA TCCTGAACTC GAGGGAAACT TTATGCATGA GGAAGGAGAA
    TTAAAAGAAG ACAGGGAGGA AAAGGAAGTA GAAATCCCTG AGAATGCAAA CACTGAAAAG
    GTATCCCTGG AAAGACTACT CAAAGTGTTC CAAGGAGGAA AAGAAGTACA GGATGCTAAC
    AGATTCACCA GCCACCTACA GATAGAGAAC ACTTACTCAG AGAACTTCGT AAAAGTATTT
    CAAGACCTAG GTGCCAAGGA TCTGGAGCCA GTTGAAGTTG CTGTTCTCTT GAAGCATCCT
    TTTATTCAAG ACCTGATTTC AAATTATTCA GACTATAAAA TCCCTGATAT TAAAATAATT
    CTCCAAAGGA GTGAGCATGT ACAAGGAAGT GATGGAGAAA GATCTCCTTC AAGACTTACA
    GAGGAAAAGA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36163
    Clone ID Related Accession (Same CDS sequence) XM_014113144.1
    Accession Version XM_014113144.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4980bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product sperm flagellar protein 2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTCGGAGA TTCTGTGCCA GTGGCTCAAC CAGGAGGTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAAT TCTGCACAAG
    TTTGAACTTC AGGGTGATTT TTCGGAATTT TCAGAAAGCA GAGTTTCAAA TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCGACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCACA ACATCATAAC GGAAAAGCCT GGGGCGGCAA CCAAACTCTT GTATCAGTTG
    TACATTGTTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAATAGAAAT GCAAACTATG
    CAACCCCTGG CAAACGTCAG ATACCAAAGT ATGAAAAGTG AGGCTTTTCG AGATCAACGC
    CTGAGCAGGA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCTG CATCAAATCG TACTTTGAGA GCACTGGAGG CCCAAAAAAT GATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAG ATTAAGAAGT TTGAAGCTTT AATAAAAAAG
    GATCTCCAGG CCAAAGAAAG TGCCTCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACT
    ACTGATTTGT TAAACACTTA TTCGGATGAT GACTACATTA AAAAAATCCA AAAGCGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT ATTAATGGAC
    CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCGTATCGGG AGGAGCAGCT GATTAACCGG
    CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATCTCTGTGC AGCTCATGCA TGTTCGGCAT
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCCGAGAAA AACAGTATGA GGAAAGACGA
    CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT
    GATTTTCAGG AACAAGCCAT TAGAGAGAAG GAAATTCATG AACAGATCAC TGTGGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATACA ATATGTGCAG AAATTTTGGA TCAAATACTT
    GATCTTTCCA CTAAAGTGTC AGACTACCGG ATGTTGACAA ATAATTTGAT TCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT AATGGAAAGC CCATATACAA GCAAGCCTCT
    ATTAAGCAAC TACCAGCCAC GCCCTCTGCA GAACAAGTTA TAGAACTGGA GAAGAGGGAC
    TTGCTGGATA GCAATGATTA TGAAGAATAT AAGAATATGG TTGGAGAGTG GGCTTTACCA
    GAAGAAATGG TTGATAATTT ACCACCCTCC AACAATACCA TATTGGGTCA TGTGATTCAC
    AGATTAATTG AAAAATCTCA TCCTCCTCAA GTCAAATCAA CAGTACTAGA ATTACCTTCA
    TTTGCTATTA AAGGGTGCTT AGTTGGAAAA ACATTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTACAAA AAGACTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT CCAAGAAGCA
    ATCAAAGCAT TCCATGACAA TGAAAAAATC AGTGAGGCTC TACCAATTGA GAAAGAAGCT
    GAAGAAAAGT CTCTACCAGT TCTGCAAGAG AAGAACAAAG AAAGCCAGGA TCTACAGAAT
    GCATTTTCAC CTCATCCAGT TTCAGAAGAA GCGTTACCAG AAACAGAAGA TAAAACTATA
    CTTAGTACTG ATACAAATAA AACTCCAAAA GCTGAAGAAG TCAAATCAAG TGATAGTTTC
    TCTGAACTGA CTGTGCGGGC TCAGCTTGGT GCAAAATCAG AAAAGATGCT GAAGAAAGGA
    AAGAGCATTT CTGACATGTT GCTAGTTAAT ATCTTGGTAA ATGCTATTAA TGAGGTACCT
    GTGGATCAAG GCTGGGTCTT GGATGGTTTT CCAATGACGT TAAACCAAGC AAAGCTTCTG
    GAGGAAGCTC TCACGGGCTA CAACAGAGAG GCCCTAGAAC TGGAAGAAAA AAAAACACAA
    ATACCCACAT TGGCTGTTGA TGCTAGCACC TCCAAAGAAG TGCCTCTTCC CCCTTCTGCA
    TTTGACTTTG TCATGTTATT AGATATTTCA GATAATTCCT TGCTGGACCG CATGAATTAC
    ACAATGGCTG AAGCATTTTC AAGTGAAACT TCTCATGAAG ACATCACTCA ACGTGTAACT
    GCTGAAAAGC AAGATATGAA TGAGGACCAG AATTTAAGAG ACCAGATTCA GCACAGAATT
    ATAGGCTTCT TGGACAACTG GCCTTTGTTG GAGCAATGGT TTACAGAGCC AGAAAATATT
    TTGATAAATA TCAATGCTGA AGTAGATAAA GAGTCTTTAT GCAATAAAGT AAAAGAAATG
    TTTATGACTG AAATAATGAA AAAAGAGAAT AAAGTTAAAA AGAAATTAGA AGAAAAGGAA
    GCTGAGAAAA AAGAAGTTCC CCTGGGTGAG CCTCCGCCTC CTCCTCCTCC CCCTCCCCCT
    CCTCCTCCTG AGCCAGAAAA AGAGAAGGAA ACCCATCCTC AACGAGAGCA TTCAAAAACT
    CCTACTGCAA AAGGGAAACC ACCAGCAGGT TCTCCTAAAG GAAAATCACC AGGAGGGAAA
    GGACCACTAA AGAAATCACC TGCTGATTCT ACAGACATGT CACCTGTTCC CATAGTGCCA
    CCACCACCCA AGCCAGGATC AGAAGAATGG GTCTACGTGA ATGAACCAAT TCCTGAGGAA
    ATGCCTTCAT TTTTAGTGCC TTACTGGGAA CTAATCGAAA ATTCCTACAT AAACTCCATC
    AAAACAGTAC TCAGGCATCT GAGAGAAGAA CAGCATAGTG TGGTTGCTTA CTTATACGAG
    GTTAGAACAA ATTTCCAGCA GTTTCTATGG CGTCCAGATC ACAAGCAAGA TTTTGTATCT
    CAATGGCAGG CTGATTTCAA TGCTCTTCCT GATGACCTGT GGGATGATGA AGAAACAAAG
    TCTGAACTAC ACCAAAGAGT AAATGATTTA AGAGACCGCC TGTGGGACAT CTGTGATGCC
    CGGAAGGAAG AAGCAGAGCA GGAGCGTCAG GATATCATTA ACGAGAGCTG GTTGCAAGAC
    TCAATCGGAA TAACGATGAA CCATTTCTTC TCACTAATGC AGACAGAACT GAACCGTTTC
    CAAGATACAA AGAGACTCCT TCAAGATTAT TACAGGGGAA TGGAATGCAA AATTCCAATC
    GATGACAGTA AGAGATTTAC TCGAATCCCG TTGGTCCAGC TGGATAGTAG TAGAGACATT
    TTGGAAAGCC AACTCAGAAT TCCTCTAATT CCACGAATAT CCATTTCCCC GGATTCCGCT
    ATGACCAAAC CAAAAACAAA GGCCATACTA AAGGGCAAGA TCGATTACTC GCTGGAAAAT
    GTGGAGTTAA ACTTTGAGGC AGATGAGAAG TTGGTGATGG ACACCTGGCA GCAGGCTTCT
    TTAGCAATAT CTCACATGGT GGCTGCTGAA ATTCATCAGA GGCTCATGGA AGAAGAAAAG
    GAAAACCAAC CAGCAGACAC AAAAGAAAAA TCTCCTCAGA TGGGGACAAA TAAAAAAGCC
    AAAGAGGAAC CACCCAAGAA AGAAAAGGCA GACAAAAAAG CAAAAGGCAG ATCCCCGCCG
    ACAGCAGAAG CCACGCCTGT GACAGTCACA GCAGAGGAGA TTACCCAAAA GGAGAAGAGA
    AACGAACTGA TGCTCAAAAT CAAGGAAGAG CACCTCGCTG CCCTGCAGTT TGAAGAGATA
    GCCACACAGT TCCGGCTTGA ACTGATAAAG ACAAAAGCAT TGGCTTTCCT TGAAGATTTA
    CTAACAAAGG TGGTCGATGT ATATAAACTC TTGGAAAAGT GGCTTGGTGA GAGGTACTTG
    AATGAAATGG CCAGTGTGGA AAAATTAACT GAGGTGGCCC GTTATCACAT TGAAACATCA
    ACAAAAATTC AGAATGAACT TTACCTAGAC CAAGAAGATT TCTTCATTAA TGGAGATCTG
    AAAGTCTTTC CAGATCCCCC TCCCCCAGTG CGCCCCCCAC CTGTGGAAAA GGAAGAAAAT
    GGCACCCTGA CCATCGAACA GCTTGACAGT CTTAAAGATC AGTTCTTAGA TATTGCACCC
    AAAGGCATAA TAGGACATAA AGCATTTACT GACATTCTGC TGGATCTGGT AACCCTGAAC
    CTTGGAACAA ACAACTTACC TAGTAGTTGG ATGCACCTCA CGCAACCAGA ATTGCAGGAG
    TTAACATCTT TAGTAATAGT AAATTCGGAG TTTGTGGACT GGCGGAAATT CCTATTAGTA
    ATCTCGATGC CTTGGCCCAT CCCCATGGAG GAAGAGCTCC TCGAAACCCT GCAGAGGTTC
    AAGGCGGTGG ATGAAGAGCA GTTGGGCACC ATCACTTTTG AGCAGTATAT GCAGGCTGGT
    CTGTGGTTTA CAGGAGATGA ATATATAAAA ATTCCAGAAA ATCCTCTTGA ACCTCTGCCG
    TTCAATAGGC AGGAGCATCT TATAGAGTTT TTCTTTAGGC TATTTGCTGA CCATGAGAAG
    GATCCACCTC AACTTGACTA CACACAGATG CTGCTCTATT TTGCTTGCCA TCCAGATGCT
    ATGGAAGGGG TCTACAGGGC CCTCAGTGTG GCCATAGGAA CTCATGTCTT CCAACCAGTC
    GAAACTATTG CTATTATTGG AGAAAAGAAC TTCGTAAAAG TATTTCAAGA CCTAGGTGCC
    AAGGATCTGG AGCCAGTTGA AGTTGCTGTT CTCTTGAAGC ATCCTTTTAT TCAAGACCTG
    ATTTCAAATT ATTCAGACTA TAAAATCCCT GATATTAAAA TAATTCTCCA AAGGAGTGAG
    CATGTACAAG GAAGTGATGG AGAAAGATCT CCTTCAAGAC TTACAGAGGA AAAGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968619.1
    CDS159..5138
    Translation

    Target ORF information:

    RefSeq Version XM_014113144.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113144.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTCGGAGA TTCTGTGCCA GTGGCTCAAC CAGGAGGTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAAT TCTGCACAAG
    TTTGAACTTC AGGGTGATTT TTCGGAATTT TCAGAAAGCA GAGTTTCAAA TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCGACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCACA ACATCATAAC GGAAAAGCCT GGGGCGGCAA CCAAACTCTT GTATCAGTTG
    TACATTGTTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAATAGAAAT GCAAACTATG
    CAACCCCTGG CAAACGTCAG ATACCAAAGT ATGAAAAGTG AGGCTTTTCG AGATCAACGC
    CTGAGCAGGA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCTG CATCAAATCG TACTTTGAGA GCACTGGAGG CCCAAAAAAT GATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAG ATTAAGAAGT TTGAAGCTTT AATAAAAAAG
    GATCTCCAGG CCAAAGAAAG TGCCTCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACT
    ACTGATTTGT TAAACACTTA TTCGGATGAT GACTACATTA AAAAAATCCA AAAGCGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT ATTAATGGAC
    CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCGTATCGGG AGGAGCAGCT GATTAACCGG
    CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATCTCTGTGC AGCTCATGCA TGTTCGGCAT
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCCGAGAAA AACAGTATGA GGAAAGACGA
    CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT
    GATTTTCAGG AACAAGCCAT TAGAGAGAAG GAAATTCATG AACAGATCAC TGTGGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATACA ATATGTGCAG AAATTTTGGA TCAAATACTT
    GATCTTTCCA CTAAAGTGTC AGACTACCGG ATGTTGACAA ATAATTTGAT TCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT AATGGAAAGC CCATATACAA GCAAGCCTCT
    ATTAAGCAAC TACCAGCCAC GCCCTCTGCA GAACAAGTTA TAGAACTGGA GAAGAGGGAC
    TTGCTGGATA GCAATGATTA TGAAGAATAT AAGAATATGG TTGGAGAGTG GGCTTTACCA
    GAAGAAATGG TTGATAATTT ACCACCCTCC AACAATACCA TATTGGGTCA TGTGATTCAC
    AGATTAATTG AAAAATCTCA TCCTCCTCAA GTCAAATCAA CAGTACTAGA ATTACCTTCA
    TTTGCTATTA AAGGGTGCTT AGTTGGAAAA ACATTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTACAAA AAGACTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT CCAAGAAGCA
    ATCAAAGCAT TCCATGACAA TGAAAAAATC AGTGAGGCTC TACCAATTGA GAAAGAAGCT
    GAAGAAAAGT CTCTACCAGT TCTGCAAGAG AAGAACAAAG AAAGCCAGGA TCTACAGAAT
    GCATTTTCAC CTCATCCAGT TTCAGAAGAA GCGTTACCAG AAACAGAAGA TAAAACTATA
    CTTAGTACTG ATACAAATAA AACTCCAAAA GCTGAAGAAG TCAAATCAAG TGATAGTTTC
    TCTGAACTGA CTGTGCGGGC TCAGCTTGGT GCAAAATCAG AAAAGATGCT GAAGAAAGGA
    AAGAGCATTT CTGACATGTT GCTAGTTAAT ATCTTGGTAA ATGCTATTAA TGAGGTACCT
    GTGGATCAAG GCTGGGTCTT GGATGGTTTT CCAATGACGT TAAACCAAGC AAAGCTTCTG
    GAGGAAGCTC TCACGGGCTA CAACAGAGAG GCCCTAGAAC TGGAAGAAAA AAAAACACAA
    ATACCCACAT TGGCTGTTGA TGCTAGCACC TCCAAAGAAG TGCCTCTTCC CCCTTCTGCA
    TTTGACTTTG TCATGTTATT AGATATTTCA GATAATTCCT TGCTGGACCG CATGAATTAC
    ACAATGGCTG AAGCATTTTC AAGTGAAACT TCTCATGAAG ACATCACTCA ACGTGTAACT
    GCTGAAAAGC AAGATATGAA TGAGGACCAG AATTTAAGAG ACCAGATTCA GCACAGAATT
    ATAGGCTTCT TGGACAACTG GCCTTTGTTG GAGCAATGGT TTACAGAGCC AGAAAATATT
    TTGATAAATA TCAATGCTGA AGTAGATAAA GAGTCTTTAT GCAATAAAGT AAAAGAAATG
    TTTATGACTG AAATAATGAA AAAAGAGAAT AAAGTTAAAA AGAAATTAGA AGAAAAGGAA
    GCTGAGAAAA AAGAAGTTCC CCTGGGTGAG CCTCCGCCTC CTCCTCCTCC CCCTCCCCCT
    CCTCCTCCTG AGCCAGAAAA AGAGAAGGAA ACCCATCCTC AACGAGAGCA TTCAAAAACT
    CCTACTGCAA AAGGGAAACC ACCAGCAGGT TCTCCTAAAG GAAAATCACC AGGAGGGAAA
    GGACCACTAA AGAAATCACC TGCTGATTCT ACAGACATGT CACCTGTTCC CATAGTGCCA
    CCACCACCCA AGCCAGGATC AGAAGAATGG GTCTACGTGA ATGAACCAAT TCCTGAGGAA
    ATGCCTTCAT TTTTAGTGCC TTACTGGGAA CTAATCGAAA ATTCCTACAT AAACTCCATC
    AAAACAGTAC TCAGGCATCT GAGAGAAGAA CAGCATAGTG TGGTTGCTTA CTTATACGAG
    GTTAGAACAA ATTTCCAGCA GTTTCTATGG CGTCCAGATC ACAAGCAAGA TTTTGTATCT
    CAATGGCAGG CTGATTTCAA TGCTCTTCCT GATGACCTGT GGGATGATGA AGAAACAAAG
    TCTGAACTAC ACCAAAGAGT AAATGATTTA AGAGACCGCC TGTGGGACAT CTGTGATGCC
    CGGAAGGAAG AAGCAGAGCA GGAGCGTCAG GATATCATTA ACGAGAGCTG GTTGCAAGAC
    TCAATCGGAA TAACGATGAA CCATTTCTTC TCACTAATGC AGACAGAACT GAACCGTTTC
    CAAGATACAA AGAGACTCCT TCAAGATTAT TACAGGGGAA TGGAATGCAA AATTCCAATC
    GATGACAGTA AGAGATTTAC TCGAATCCCG TTGGTCCAGC TGGATAGTAG TAGAGACATT
    TTGGAAAGCC AACTCAGAAT TCCTCTAATT CCACGAATAT CCATTTCCCC GGATTCCGCT
    ATGACCAAAC CAAAAACAAA GGCCATACTA AAGGGCAAGA TCGATTACTC GCTGGAAAAT
    GTGGAGTTAA ACTTTGAGGC AGATGAGAAG TTGGTGATGG ACACCTGGCA GCAGGCTTCT
    TTAGCAATAT CTCACATGGT GGCTGCTGAA ATTCATCAGA GGCTCATGGA AGAAGAAAAG
    GAAAACCAAC CAGCAGACAC AAAAGAAAAA TCTCCTCAGA TGGGGACAAA TAAAAAAGCC
    AAAGAGGAAC CACCCAAGAA AGAAAAGGCA GACAAAAAAG CAAAAGGCAG ATCCCCGCCG
    ACAGCAGAAG CCACGCCTGT GACAGTCACA GCAGAGGAGA TTACCCAAAA GGAGAAGAGA
    AACGAACTGA TGCTCAAAAT CAAGGAAGAG CACCTCGCTG CCCTGCAGTT TGAAGAGATA
    GCCACACAGT TCCGGCTTGA ACTGATAAAG ACAAAAGCAT TGGCTTTCCT TGAAGATTTA
    CTAACAAAGG TGGTCGATGT ATATAAACTC TTGGAAAAGT GGCTTGGTGA GAGGTACTTG
    AATGAAATGG CCAGTGTGGA AAAATTAACT GAGGTGGCCC GTTATCACAT TGAAACATCA
    ACAAAAATTC AGAATGAACT TTACCTAGAC CAAGAAGATT TCTTCATTAA TGGAGATCTG
    AAAGTCTTTC CAGATCCCCC TCCCCCAGTG CGCCCCCCAC CTGTGGAAAA GGAAGAAAAT
    GGCACCCTGA CCATCGAACA GCTTGACAGT CTTAAAGATC AGTTCTTAGA TATTGCACCC
    AAAGGCATAA TAGGACATAA AGCATTTACT GACATTCTGC TGGATCTGGT AACCCTGAAC
    CTTGGAACAA ACAACTTACC TAGTAGTTGG ATGCACCTCA CGCAACCAGA ATTGCAGGAG
    TTAACATCTT TAGTAATAGT AAATTCGGAG TTTGTGGACT GGCGGAAATT CCTATTAGTA
    ATCTCGATGC CTTGGCCCAT CCCCATGGAG GAAGAGCTCC TCGAAACCCT GCAGAGGTTC
    AAGGCGGTGG ATGAAGAGCA GTTGGGCACC ATCACTTTTG AGCAGTATAT GCAGGCTGGT
    CTGTGGTTTA CAGGAGATGA ATATATAAAA ATTCCAGAAA ATCCTCTTGA ACCTCTGCCG
    TTCAATAGGC AGGAGCATCT TATAGAGTTT TTCTTTAGGC TATTTGCTGA CCATGAGAAG
    GATCCACCTC AACTTGACTA CACACAGATG CTGCTCTATT TTGCTTGCCA TCCAGATGCT
    ATGGAAGGGG TCTACAGGGC CCTCAGTGTG GCCATAGGAA CTCATGTCTT CCAACCAGTC
    GAAACTATTG CTATTATTGG AGAAAAGAAC TTCGTAAAAG TATTTCAAGA CCTAGGTGCC
    AAGGATCTGG AGCCAGTTGA AGTTGCTGTT CTCTTGAAGC ATCCTTTTAT TCAAGACCTG
    ATTTCAAATT ATTCAGACTA TAAAATCCCT GATATTAAAA TAATTCTCCA AAGGAGTGAG
    CATGTACAAG GAAGTGATGG AGAAAGATCT CCTTCAAGAC TTACAGAGGA AAAGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36164
    Clone ID Related Accession (Same CDS sequence) XM_014113145.1
    Accession Version XM_014113145.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5142bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product sperm flagellar protein 2 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGGAAAG ACGACTTAAA GATTTCCAGG ATGCTCTTGA TCGAGAAGCG TTTGATTCCA 
    TATAAGTTGA TGCATGATTG GAAGGAACTG TTTTTTAATG GAAAGCCCAT ATACAAGCAA
    GCCTCTATTA AGCAACTACC AGCCACGCCC TCTGCAGAAC AAGTTATAGA ACTGGAGAAG
    AGGGACTTGC TGGATAGCAA TGATTATGAA GAATATAAGA ATATGGTTGG AGAGTGGGCT
    TTACCAGAAG AAATGGTTGA TAATTTACCA CCCTCCAACA ATACCATATT GGGTCATGTG
    ATTCACAGAT TAATTGAAAA ATCTCATCCT CCTCAAGTCA AATCAACAGT ACTAGAATTA
    CCTTCATTTG CTATTAAAGG GTGCTTAGTT GGAAAAACAT TTAGTGGAAA AACTACTGCT
    CTAAAGGCTC TACAAAAAGA CTTTCCTATT CAGGTACTTT CTATTGACAC TCTTGTCCAA
    GAAGCAATCA AAGCATTCCA TGACAATGAA AAAATCAGTG AGGCTCTACC AATTGAGAAA
    GAAGCTGAAG AAAAGTCTCT ACCAGTTCTG CAAGAGAAGA ACAAAGAAAG CCAGGATCTA
    CAGAATGCAT TTTCACCTCA TCCAGTTTCA GAAGAAGCGT TACCAGAAAC AGAAGATAAA
    ACTATACTTA GTACTGATAC AAATAAAACT CCAAAAGCTG AAGAAGTCAA ATCAAGTGAT
    AGTTTCTCTG AACTGACTGT GCGGGCTCAG CTTGGTGCAA AATCAGAAAA GATGCTGAAG
    AAAGGAAAGA GCATTTCTGA CATGTTGCTA GTTAATATCT TGGTAAATGC TATTAATGAG
    GTACCTGTGG ATCAAGGCTG GGTCTTGGAT GGTTTTCCAA TGACGTTAAA CCAAGCAAAG
    CTTCTGGAGG AAGCTCTCAC GGGCTACAAC AGAGAGGCCC TAGAACTGGA AGAAAAAAAA
    ACACAAATAC CCACATTGGC TGTTGATGCT AGCACCTCCA AAGAAGTGCC TCTTCCCCCT
    TCTGCATTTG ACTTTGTCAT GTTATTAGAT ATTTCAGATA ATTCCTTGCT GGACCGCATG
    AATTACACAA TGGCTGAAGC ATTTTCAAGT GAAACTTCTC ATGAAGACAT CACTCAACGT
    GTAACTGCTG AAAAGCAAGA TATGAATGAG GACCAGAATT TAAGAGACCA GATTCAGCAC
    AGAATTATAG GCTTCTTGGA CAACTGGCCT TTGTTGGAGC AATGGTTTAC AGAGCCAGAA
    AATATTTTGA TAAATATCAA TGCTGAAGTA GATAAAGAGT CTTTATGCAA TAAAGTAAAA
    GAAATGTTTA TGACTGAAAT AATGAAAAAA GAGAATAAAG TTAAAAAGAA ATTAGAAGAA
    AAGGAAGCTG AGAAAAAAGA AGTTCCCCTG GGTGAGCCTC CGCCTCCTCC TCCTCCCCCT
    CCCCCTCCTC CTCCTGAGCC AGAAAAAGAG AAGGAAACCC ATCCTCAACG AGAGCATTCA
    AAAACTCCTA CTGCAAAAGG GAAACCACCA GCAGGTTCTC CTAAAGGAAA ATCACCAGGA
    GGGAAAGGAC CACTAAAGAA ATCACCTGCT GATTCTACAG ACATGTCACC TGTTCCCATA
    GTGCCACCAC CACCCAAGCC AGGATCAGAA GAATGGGTCT ACGTGAATGA ACCAATTCCT
    GAGGAAATGC CTTCATTTTT AGTGCCTTAC TGGGAACTAA TCGAAAATTC CTACATAAAC
    TCCATCAAAA CAGTACTCAG GCATCTGAGA GAAGAACAGC ATAGTGTGGT TGCTTACTTA
    TACGAGGTTA GAACAAATTT CCAGCAGTTT CTATGGCGTC CAGATCACAA GCAAGATTTT
    GTATCTCAAT GGCAGGCTGA TTTCAATGCT CTTCCTGATG ACCTGTGGGA TGATGAAGAA
    ACAAAGTCTG AACTACACCA AAGAGTAAAT GATTTAAGAG ACCGCCTGTG GGACATCTGT
    GATGCCCGGA AGGAAGAAGC AGAGCAGGAG CGTCAGGATA TCATTAACGA GAGCTGGTTG
    CAAGACTCAA TCGGAATAAC GATGAACCAT TTCTTCTCAC TAATGCAGAC AGAACTGAAC
    CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACA GGGGAATGGA ATGCAAAATT
    CCAATCGATG ACAGTAAGAG ATTTACTCGA ATCCCGTTGG TCCAGCTGGA TAGTAGTAGA
    GACATTTTGG AAAGCCAACT CAGAATTCCT CTAATTCCAC GAATATCCAT TTCCCCGGAT
    TCCGCTATGA CCAAACCAAA AACAAAGGCC ATACTAAAGG GCAAGATCGA TTACTCGCTG
    GAAAATGTGG AGTTAAACTT TGAGGCAGAT GAGAAGTTGG TGATGGACAC CTGGCAGCAG
    GCTTCTTTAG CAATATCTCA CATGGTGGCT GCTGAAATTC ATCAGAGGCT CATGGAAGAA
    GAAAAGGAAA ACCAACCAGC AGACACAAAA GAAAAATCTC CTCAGATGGG GACAAATAAA
    AAAGCCAAAG AGGAACCACC CAAGAAAGAA AAGGCAGACA AAAAAGCAAA AGGCAGATCC
    CCGCCGACAG CAGAAGCCAC GCCTGTGACA GTCACAGCAG AGGAGATTAC CCAAAAGGAG
    AAGAGAAACG AACTGATGCT CAAAATCAAG GAAGAGCACC TCGCTGCCCT GCAGTTTGAA
    GAGATAGCCA CACAGTTCCG GCTTGAACTG ATAAAGACAA AAGCATTGGC TTTCCTTGAA
    GATTTACTAA CAAAGGTGGT CGATGTATAT AAACTCTTGG AAAAGTGGCT TGGTGAGAGG
    TACTTGAATG AAATGGCCAG TGTGGAAAAA TTAACTGAGG TGGCCCGTTA TCACATTGAA
    ACATCAACAA AAATTCAGAA TGAACTTTAC CTAGACCAAG AAGATTTCTT CATTAATGGA
    GATCTGAAAG TCTTTCCAGA TCCCCCTCCC CCAGTGCGCC CCCCACCTGT GGAAAAGGAA
    GAAAATGGCA CCCTGACCAT CGAACAGCTT GACAGTCTTA AAGATCAGTT CTTAGATATT
    GCACCCAAAG GCAGGGATCC TCCAGGTGAC ATGAACCGAG TCACTGAAAA GCAGAAAAGG
    CTGCAGAAGC TTGACCATTA CCTTCCAAAA AGGAACGATC AATTTTTGAA AATCTGGCTG
    AAAAAATACC CTTGGCTTAT ATATGATGAG ATGTTAAATC TTATGTTCTG TGGCCTGTGT
    CGTAAACATG GAGTGAAGTC AAAGGGAAAT CAAGTGAGTT TTTTTTATGG CACTGACAAC
    TTTAGGACAG AATTTCTCAA TGCACATCAT CTCAGTGAGG CTCATGCCAA GGCTTCACTA
    ATGGAAGCAA CAAATGGTTC TCCAGTGAAT GTCGCCACCA CAGAGCTCAT GGTGACAACC
    ATGAGCAAAA TCACCCTCGG GAGAGTAGAG AACTTATTCA GATCATGCCA TGCTATCGCC
    AAGACGGGAC GTCCTCTCAA AGACTTTATC TGGATGTGCA AGTTGGATGA TATGAAGGGG
    GTCGATATCG GCCCCGTATT CAGAACTAAC AAATCAGCAA GAACCTTTAC ATATTTTATT
    GCTGAAGTGG AACGGAAAAA TCTTAGGGAG AAACTAGAAC AAAGTAAATT TTTCTCTGTC
    CTCAGTGACG GAATCACAGA CAGTTCAACC AAGGCGGCTG AACTTGTCTA CGTGCAGTTT
    GCACACGCGG GAAAAGTGCA TTGCCAAATT GCTGGGGTAC AGACAGTAGA AAGGGAGGAT
    CCTTTGGCAA TAAGAGATGC CATTGAAAGA ACTCTAGAGA GAAATCTTCA ACTTAAGCTA
    TCAGACCAAG ACTGGGCCAA GAAACTGGTT GGTTTTGGAA GTGATGGTGC CCTTGGCTCA
    GGGAGAGAAA ACAGCAGGGT GGCCCTGCTT TTAAGAGAAA TCCAGCCATG TGCTCTGACT
    GTATACTGCT TTGCACACCA TCTTGAACTG TCTTACAAGG CGGTGTTCCA AAGCGTTCCC
    CTGTACAACG ACGTCAGAAA TCTTCTTCAC AGCATCTACC ACTTCTACCA CAGTTCCCCT
    CTGCACAGGA GTTCTCTGGT AACTGCTTTC AAAGGCCTTC ACCTTCGACC TGTGATGCCT
    TCTCAAGTAG GGGGCAGGAG GTGGCTTCAG GAACTACAGG CAGCTCTCCA GAACTTTCTC
    AAGGGATATC CTGCAATAGT CCAGCAACTG CATTCGGGAG ATGAAGGCAG GAGTGACACC
    AGTCATCAGA AAGCCAAGGA ACTCCTGGGG TTCCTTCTCC AAGCAAACAT TGTTAAATTT
    ATCCACTTCT TAGTGGATGT CATTAATGTC CTTAGCATTC TTTCCCGTGT CAATCAAAAC
    AGAAATTCTT CCATTGCTGA TGTATTTGCC ACATTAGAAT CAACACTGGA AATGCTCAGA
    ATATATCAAA CAAGACCAGG ACCAAAGGAA CGTTGGGTGG ATTCGGTCAC GCACTTTCAT
    GGCAACTGCC TGGGAGGAAA GGGAGACATT TCTGATGTGA GAAGAGTAGT CTTAACACAT
    CTTATCAAGA GGCTGCAAGG TTGCTTCAGA GATGCCAGCC AAGATGTGCT GAAGGCAACC
    ATGATTGGAA GCTTTAAACT GTGGCCTGCG AAGATAAATC AAGAATTTGG AGAAAAAGAG
    GTATCCACCC TGATTGCCCA TTATGAACCA GTACTGGAAG CTGCCAACGT GGAAATCGAC
    GAAGTGGACG CTGAGTGGAG CATGCTGAAA TTAGAGATTT ATGCTAGATT TCAGAACATC
    CGCAAATTGA CCTGGGATTT TGTGAATTCT GTTTACTTAC ACAAGTATCC AAATATCTTG
    ACGTTAGTGG ACCTCGTGCT GACCCTCCCT GCAAGTTCCG CGGAGGCAGA ACGTGGCTTT
    AGTCAGATGA AACTCACCAA GTCACAAATG CGTGCCAAAA TCAAGGCTGA AAGTATGACG
    GATCTCCTTG TAATTCAGTT GAATTCTCCG GACATTAATA ACTTTGACCC TCGGAAAGCT
    ATCCATTTAT GGAACACAAG AACACCATCT TCAGCTGATG ACACAAGACC TAATTCTGCT
    TATTCATCAA ACTCCGAAAG CCATGATGAA AGCGATTCGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968620.1
    CDS45..5186
    Translation

    Target ORF information:

    RefSeq Version XM_014113145.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113145.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGGAAAG ACGACTTAAA GATTTCCAGG ATGCTCTTGA TCGAGAAGCG TTTGATTCCA 
    TATAAGTTGA TGCATGATTG GAAGGAACTG TTTTTTAATG GAAAGCCCAT ATACAAGCAA
    GCCTCTATTA AGCAACTACC AGCCACGCCC TCTGCAGAAC AAGTTATAGA ACTGGAGAAG
    AGGGACTTGC TGGATAGCAA TGATTATGAA GAATATAAGA ATATGGTTGG AGAGTGGGCT
    TTACCAGAAG AAATGGTTGA TAATTTACCA CCCTCCAACA ATACCATATT GGGTCATGTG
    ATTCACAGAT TAATTGAAAA ATCTCATCCT CCTCAAGTCA AATCAACAGT ACTAGAATTA
    CCTTCATTTG CTATTAAAGG GTGCTTAGTT GGAAAAACAT TTAGTGGAAA AACTACTGCT
    CTAAAGGCTC TACAAAAAGA CTTTCCTATT CAGGTACTTT CTATTGACAC TCTTGTCCAA
    GAAGCAATCA AAGCATTCCA TGACAATGAA AAAATCAGTG AGGCTCTACC AATTGAGAAA
    GAAGCTGAAG AAAAGTCTCT ACCAGTTCTG CAAGAGAAGA ACAAAGAAAG CCAGGATCTA
    CAGAATGCAT TTTCACCTCA TCCAGTTTCA GAAGAAGCGT TACCAGAAAC AGAAGATAAA
    ACTATACTTA GTACTGATAC AAATAAAACT CCAAAAGCTG AAGAAGTCAA ATCAAGTGAT
    AGTTTCTCTG AACTGACTGT GCGGGCTCAG CTTGGTGCAA AATCAGAAAA GATGCTGAAG
    AAAGGAAAGA GCATTTCTGA CATGTTGCTA GTTAATATCT TGGTAAATGC TATTAATGAG
    GTACCTGTGG ATCAAGGCTG GGTCTTGGAT GGTTTTCCAA TGACGTTAAA CCAAGCAAAG
    CTTCTGGAGG AAGCTCTCAC GGGCTACAAC AGAGAGGCCC TAGAACTGGA AGAAAAAAAA
    ACACAAATAC CCACATTGGC TGTTGATGCT AGCACCTCCA AAGAAGTGCC TCTTCCCCCT
    TCTGCATTTG ACTTTGTCAT GTTATTAGAT ATTTCAGATA ATTCCTTGCT GGACCGCATG
    AATTACACAA TGGCTGAAGC ATTTTCAAGT GAAACTTCTC ATGAAGACAT CACTCAACGT
    GTAACTGCTG AAAAGCAAGA TATGAATGAG GACCAGAATT TAAGAGACCA GATTCAGCAC
    AGAATTATAG GCTTCTTGGA CAACTGGCCT TTGTTGGAGC AATGGTTTAC AGAGCCAGAA
    AATATTTTGA TAAATATCAA TGCTGAAGTA GATAAAGAGT CTTTATGCAA TAAAGTAAAA
    GAAATGTTTA TGACTGAAAT AATGAAAAAA GAGAATAAAG TTAAAAAGAA ATTAGAAGAA
    AAGGAAGCTG AGAAAAAAGA AGTTCCCCTG GGTGAGCCTC CGCCTCCTCC TCCTCCCCCT
    CCCCCTCCTC CTCCTGAGCC AGAAAAAGAG AAGGAAACCC ATCCTCAACG AGAGCATTCA
    AAAACTCCTA CTGCAAAAGG GAAACCACCA GCAGGTTCTC CTAAAGGAAA ATCACCAGGA
    GGGAAAGGAC CACTAAAGAA ATCACCTGCT GATTCTACAG ACATGTCACC TGTTCCCATA
    GTGCCACCAC CACCCAAGCC AGGATCAGAA GAATGGGTCT ACGTGAATGA ACCAATTCCT
    GAGGAAATGC CTTCATTTTT AGTGCCTTAC TGGGAACTAA TCGAAAATTC CTACATAAAC
    TCCATCAAAA CAGTACTCAG GCATCTGAGA GAAGAACAGC ATAGTGTGGT TGCTTACTTA
    TACGAGGTTA GAACAAATTT CCAGCAGTTT CTATGGCGTC CAGATCACAA GCAAGATTTT
    GTATCTCAAT GGCAGGCTGA TTTCAATGCT CTTCCTGATG ACCTGTGGGA TGATGAAGAA
    ACAAAGTCTG AACTACACCA AAGAGTAAAT GATTTAAGAG ACCGCCTGTG GGACATCTGT
    GATGCCCGGA AGGAAGAAGC AGAGCAGGAG CGTCAGGATA TCATTAACGA GAGCTGGTTG
    CAAGACTCAA TCGGAATAAC GATGAACCAT TTCTTCTCAC TAATGCAGAC AGAACTGAAC
    CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACA GGGGAATGGA ATGCAAAATT
    CCAATCGATG ACAGTAAGAG ATTTACTCGA ATCCCGTTGG TCCAGCTGGA TAGTAGTAGA
    GACATTTTGG AAAGCCAACT CAGAATTCCT CTAATTCCAC GAATATCCAT TTCCCCGGAT
    TCCGCTATGA CCAAACCAAA AACAAAGGCC ATACTAAAGG GCAAGATCGA TTACTCGCTG
    GAAAATGTGG AGTTAAACTT TGAGGCAGAT GAGAAGTTGG TGATGGACAC CTGGCAGCAG
    GCTTCTTTAG CAATATCTCA CATGGTGGCT GCTGAAATTC ATCAGAGGCT CATGGAAGAA
    GAAAAGGAAA ACCAACCAGC AGACACAAAA GAAAAATCTC CTCAGATGGG GACAAATAAA
    AAAGCCAAAG AGGAACCACC CAAGAAAGAA AAGGCAGACA AAAAAGCAAA AGGCAGATCC
    CCGCCGACAG CAGAAGCCAC GCCTGTGACA GTCACAGCAG AGGAGATTAC CCAAAAGGAG
    AAGAGAAACG AACTGATGCT CAAAATCAAG GAAGAGCACC TCGCTGCCCT GCAGTTTGAA
    GAGATAGCCA CACAGTTCCG GCTTGAACTG ATAAAGACAA AAGCATTGGC TTTCCTTGAA
    GATTTACTAA CAAAGGTGGT CGATGTATAT AAACTCTTGG AAAAGTGGCT TGGTGAGAGG
    TACTTGAATG AAATGGCCAG TGTGGAAAAA TTAACTGAGG TGGCCCGTTA TCACATTGAA
    ACATCAACAA AAATTCAGAA TGAACTTTAC CTAGACCAAG AAGATTTCTT CATTAATGGA
    GATCTGAAAG TCTTTCCAGA TCCCCCTCCC CCAGTGCGCC CCCCACCTGT GGAAAAGGAA
    GAAAATGGCA CCCTGACCAT CGAACAGCTT GACAGTCTTA AAGATCAGTT CTTAGATATT
    GCACCCAAAG GCAGGGATCC TCCAGGTGAC ATGAACCGAG TCACTGAAAA GCAGAAAAGG
    CTGCAGAAGC TTGACCATTA CCTTCCAAAA AGGAACGATC AATTTTTGAA AATCTGGCTG
    AAAAAATACC CTTGGCTTAT ATATGATGAG ATGTTAAATC TTATGTTCTG TGGCCTGTGT
    CGTAAACATG GAGTGAAGTC AAAGGGAAAT CAAGTGAGTT TTTTTTATGG CACTGACAAC
    TTTAGGACAG AATTTCTCAA TGCACATCAT CTCAGTGAGG CTCATGCCAA GGCTTCACTA
    ATGGAAGCAA CAAATGGTTC TCCAGTGAAT GTCGCCACCA CAGAGCTCAT GGTGACAACC
    ATGAGCAAAA TCACCCTCGG GAGAGTAGAG AACTTATTCA GATCATGCCA TGCTATCGCC
    AAGACGGGAC GTCCTCTCAA AGACTTTATC TGGATGTGCA AGTTGGATGA TATGAAGGGG
    GTCGATATCG GCCCCGTATT CAGAACTAAC AAATCAGCAA GAACCTTTAC ATATTTTATT
    GCTGAAGTGG AACGGAAAAA TCTTAGGGAG AAACTAGAAC AAAGTAAATT TTTCTCTGTC
    CTCAGTGACG GAATCACAGA CAGTTCAACC AAGGCGGCTG AACTTGTCTA CGTGCAGTTT
    GCACACGCGG GAAAAGTGCA TTGCCAAATT GCTGGGGTAC AGACAGTAGA AAGGGAGGAT
    CCTTTGGCAA TAAGAGATGC CATTGAAAGA ACTCTAGAGA GAAATCTTCA ACTTAAGCTA
    TCAGACCAAG ACTGGGCCAA GAAACTGGTT GGTTTTGGAA GTGATGGTGC CCTTGGCTCA
    GGGAGAGAAA ACAGCAGGGT GGCCCTGCTT TTAAGAGAAA TCCAGCCATG TGCTCTGACT
    GTATACTGCT TTGCACACCA TCTTGAACTG TCTTACAAGG CGGTGTTCCA AAGCGTTCCC
    CTGTACAACG ACGTCAGAAA TCTTCTTCAC AGCATCTACC ACTTCTACCA CAGTTCCCCT
    CTGCACAGGA GTTCTCTGGT AACTGCTTTC AAAGGCCTTC ACCTTCGACC TGTGATGCCT
    TCTCAAGTAG GGGGCAGGAG GTGGCTTCAG GAACTACAGG CAGCTCTCCA GAACTTTCTC
    AAGGGATATC CTGCAATAGT CCAGCAACTG CATTCGGGAG ATGAAGGCAG GAGTGACACC
    AGTCATCAGA AAGCCAAGGA ACTCCTGGGG TTCCTTCTCC AAGCAAACAT TGTTAAATTT
    ATCCACTTCT TAGTGGATGT CATTAATGTC CTTAGCATTC TTTCCCGTGT CAATCAAAAC
    AGAAATTCTT CCATTGCTGA TGTATTTGCC ACATTAGAAT CAACACTGGA AATGCTCAGA
    ATATATCAAA CAAGACCAGG ACCAAAGGAA CGTTGGGTGG ATTCGGTCAC GCACTTTCAT
    GGCAACTGCC TGGGAGGAAA GGGAGACATT TCTGATGTGA GAAGAGTAGT CTTAACACAT
    CTTATCAAGA GGCTGCAAGG TTGCTTCAGA GATGCCAGCC AAGATGTGCT GAAGGCAACC
    ATGATTGGAA GCTTTAAACT GTGGCCTGCG AAGATAAATC AAGAATTTGG AGAAAAAGAG
    GTATCCACCC TGATTGCCCA TTATGAACCA GTACTGGAAG CTGCCAACGT GGAAATCGAC
    GAAGTGGACG CTGAGTGGAG CATGCTGAAA TTAGAGATTT ATGCTAGATT TCAGAACATC
    CGCAAATTGA CCTGGGATTT TGTGAATTCT GTTTACTTAC ACAAGTATCC AAATATCTTG
    ACGTTAGTGG ACCTCGTGCT GACCCTCCCT GCAAGTTCCG CGGAGGCAGA ACGTGGCTTT
    AGTCAGATGA AACTCACCAA GTCACAAATG CGTGCCAAAA TCAAGGCTGA AAGTATGACG
    GATCTCCTTG TAATTCAGTT GAATTCTCCG GACATTAATA ACTTTGACCC TCGGAAAGCT
    ATCCATTTAT GGAACACAAG AACACCATCT TCAGCTGATG ACACAAGACC TAATTCTGCT
    TATTCATCAA ACTCCGAAAG CCATGATGAA AGCGATTCGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36165
    Clone ID Related Accession (Same CDS sequence) XM_014113146.1 , XM_014113146.2
    Accession Version XM_014113146.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5220bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product sperm flagellar protein 2 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCGGAGA TTCTGTGCCA GTGGCTCAAC CAGGAGGTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAAT TCTGCACAAG
    TTTGAACTTC AGGGTGATTT TTCGGAATTT TCAGAAAGCA GAGTTTCAAA TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCGACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCACA ACATCATAAC GGAAAAGCCT GGGGCGGCAA CCAAACTCTT GTATCAGTTG
    TACATTGTTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAATAGAAAT GCAAACTATG
    CAACCCCTGG CAAACGTCAG ATACCAAAGT ATGAAAAGTG AGGCTTTTCG AGATCAACGC
    CTGAGCAGGA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCTG CATCAAATCG TACTTTGAGA GCACTGGAGG CCCAAAAAAT GATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAG ATTAAGAAGT TTGAAGCTTT AATAAAAAAG
    GATCTCCAGG CCAAAGAAAG TGCCTCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACT
    ACTGATTTGT TAAACACTTA TTCGGATGAT GACTACATTA AAAAAATCCA AAAGCGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT ATTAATGGAC
    CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCGTATCGGG AGGAGCAGCT GATTAACCGG
    CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATCTCTGTGC AGCTCATGCA TGTTCGGCAT
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCCGAGAAA AACAGTATGA GGAAAGACGA
    CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT
    GATTTTCAGG AACAAGCCAT TAGAGAGAAG GAAATTCATG AACAGATCAC TGTGGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATACA ATATGTGCAG AAATTTTGGA TCAAATACTT
    GATCTTTCCA CTAAAGTGTC AGACTACCGG ATGTTGACAA ATAATTTGAT TCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT AATGGAAAGC CCATATACAA GCAAGCCTCT
    ATTAAGCAAC TACCAGCCAC GCCCTCTGCA GAACAAGTTA TAGAACTGGA GAAGAGGGAC
    TTGCTGGATA GCAATGATTA TGAAGAATAT AAGAATATGG TTGGAGAGTG GGCTTTACCA
    GAAGAAATGG TTGATAATTT ACCACCCTCC AACAATACCA TATTGGGTCA TGTGATTCAC
    AGATTAATTG AAAAATCTCA TCCTCCTCAA GTCAAATCAA CAGTACTAGA ATTACCTTCA
    TTTGCTATTA AAGGGTGCTT AGTTGGAAAA ACATTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTACAAA AAGACTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT CCAAGAAGCA
    ATCAAAGCAT TCCATGACAA TGAAAAAATC AGTGAGGCTC TACCAATTGA GAAAGAAGCT
    GAAGAAAAGT CTCTACCAGT TCTGCAAGAG AAGAACAAAG AAAGCCAGGA TCTACAGAAT
    GCATTTTCAC CTCATCCAGT TTCAGAAGAA GCGTTACCAG AAACAGAAGA TAAAACTATA
    CTTAGTACTG ATACAAATAA AACTCCAAAA GCTGAAGAAG TCAAATCAAG TGATAGTTTC
    TCTGAACTGA CTGTGCGGGC TCAGCTTGGT GCAAAATCAG AAAAGATGCT GAAGAAAGGA
    AAGAGCATTT CTGACATGTT GCTAGTTAAT ATCTTGGTAA ATGCTATTAA TGAGGTACCT
    GTGGATCAAG GCTGGGTCTT GGATGGTTTT CCAATGACGT TAAACCAAGC AAAGCTTCTG
    GAGGAAGCTC TCACGGGCTA CAACAGAGAG GCCCTAGAAC TGGAAGAAAA AAAAACACAA
    ATACCCACAT TGGCTGTTGA TGCTAGCACC TCCAAAGAAG TGCCTCTTCC CCCTTCTGCA
    TTTGACTTTG TCATGTTATT AGATATTTCA GATAATTCCT TGCTGGACCG CATGAATTAC
    ACAATGGCTG AAGCATTTTC AAGTGAAACT TCTCATGAAG ACATCACTCA ACGTGTAACT
    GCTGAAAAGC AAGATATGAA TGAGGACCAG AATTTAAGAG ACCAGATTCA GCACAGAATT
    ATAGGCTTCT TGGACAACTG GCCTTTGTTG GAGCAATGGT TTACAGAGCC AGAAAATATT
    TTGATAAATA TCAATGCTGA AGTAGATAAA GAGTCTTTAT GCAATAAAGT AAAAGAAATG
    TTTATGACTG AAATAATGAA AAAAGAGAAT AAAGTTAAAA AGAAATTAGA AGAAAAGGAA
    GCTGAGAAAA AAGAAGTTCC CCTGGGTGAG CCTCCGCCTC CTCCTCCTCC CCCTCCCCCT
    CCTCCTCCTG AGCCAGAAAA AGAGAAGGAA ACCCATCCTC AACGAGAGCA TTCAAAAACT
    CCTACTGCAA AAGGGAAACC ACCAGCAGGT TCTCCTAAAG GAAAATCACC AGGAGGGAAA
    GGACCACTAA AGAAATCACC TGCTGATTCT ACAGACATGT CACCTGTTCC CATAGTGCCA
    CCACCACCCA AGCCAGGATC AGAAGAATGG GTCTACGTGA ATGAACCAAT TCCTGAGGAA
    ATGCCTTCAT TTTTAGTGCC TTACTGGGAA CTAATCGAAA ATTCCTACAT AAACTCCATC
    AAAACAGTAC TCAGGCATCT GAGAGAAGAA CAGCATAGTG TGGTTGCTTA CTTATACGAG
    GTTAGAACAA ATTTCCAGCA GTTTCTATGG CGTCCAGATC ACAAGCAAGA TTTTGTATCT
    CAATGGCAGG CTGATTTCAA TGCTCTTCCT GATGACCTGT GGGATGATGA AGAAACAAAG
    TCTGAACTAC ACCAAAGAGT AAATGATTTA AGAGACCGCC TGTGGGACAT CTGTGATGCC
    CGGAAGGAAG AAGCAGAGCA GGAGCGTCAG GATATCATTA ACGAGAGCTG GTTGCAAGAC
    TCAATCGGAA TAACGATGAA CCATTTCTTC TCACTAATGC AGACAGAACT GAACCGTTTC
    CAAGATACAA AGAGACTCCT TCAAGATTAT TACAGGGGAA TGGAATGCAA AATTCCAATC
    GATGACAGTA AGAGATTTAC TCGAATCCCG TTGGTCCAGC TGGATAGTAG TAGAGACATT
    TTGGAAAGCC AACTCAGAAT TCCTCTAATT CCACGAATAT CCATTTCCCC GGATTCCGCT
    ATGACCAAAC CAAAAACAAA GGCCATACTA AAGGGCAAGA TCGATTACTC GCTGGAAAAT
    GTGGAGTTAA ACTTTGAGGC AGATGAGAAG TTGGTGATGG ACACCTGGCA GCAGGCTTCT
    TTAGCAATAT CTCACATGGT GGCTGCTGAA ATTCATCAGA GGCTCATGGA AGAAGAAAAG
    GAAAACCAAC CAGCAGACAC AAAAGAAAAA TCTCCTCAGA TGGGGACAAA TAAAAAAGCC
    AAAGAGGAAC CACCCAAGAA AGAAAAGGCA GACAAAAAAG CAAAAGGCAG ATCCCCGCCG
    ACAGCAGAAG CCACGCCTGT GACAGTCACA GCAGAGGAGA TTACCCAAAA GGAGAAGAGA
    AACGAACTGA TGCTCAAAAT CAAGGAAGAG CACCTCGCTG CCCTGCAGTT TGAAGAGATA
    GCCACACAGT TCCGGCTTGA ACTGATAAAG ACAAAAGCAT TGGCTTTCCT TGAAGATTTA
    CTAACAAAGG TGGTCGATGT ATATAAACTC TTGGAAAAGT GGCTTGGTGA GAGGTACTTG
    AATGAAATGG CCAGTGTGGA AAAATTAACT GAGGTGGCCC GTTATCACAT TGAAACATCA
    ACAAAAATTC AGAATGAACT TTACCTAGAC CAAGAAGATT TCTTCATTAA TGGAGATCTG
    AAAGTCTTTC CAGATCCCCC TCCCCCAGTG CGCCCCCCAC CTGTGGAAAA GGAAGAAAAT
    GGCACCCTGA CCATCGAACA GCTTGACAGT CTTAAAGATC AGTTCTTAGA TATTGCACCC
    AAAGGCATAA TAGGACATAA AGCATTTACT GACATTCTGC TGGATCTGGT AACCCTGAAC
    CTTGGAACAA ACAACTTACC TAGTAGTTGG ATGCACCTCA CGCAACCAGA ATTGCAGGAG
    TTAACATCTT TAGTAATAGT AAATTCGGAG TTTGTGGACT GGCGGAAATT CCTATTAGTA
    ATCTCGATGC CTTGGCCCAT CCCCATGGAG GAAGAGCTCC TCGAAACCCT GCAGAGGTTC
    AAGGCGGTGG ATGAAGAGCA GTTGGGCACC ATCACTTTTG AGCAGTATAT GCAGGCTGGT
    CTGTGGTTTA CAGGAGATGA ATATATAAAA ATTCCAGAAA ATCCTCTTGA ACCTCTGCCG
    TTCAATAGGC AGGAGCATCT TATAGAGTTT TTCTTTAGGC TATTTGCTGA CCATGAGAAG
    GATCCACCTC AACTTGACTA CACACAGATG CTGCTCTATT TTGCTTGCCA TCCAGATGCT
    ATGGAAGGGG TCTACAGGGC CCTCAGTGTG GCCATAGGAA CTCATGTCTT CCAACCAGTC
    GAAACTATTG CTATTATTGG AGAAAAGTCC TCCTCCTTGA CTGATGCAAG TCCAGTTGAG
    GAGTATCCTG AACTCGAGGG AAACTTTATG CATGAGGAAG GAGAATTAAA AGAAGACAGG
    GAGGAAAAGG AAGTAGAAAT CCCTGAGAAT GCAAACACTG AAAAGGTATC CCTGGAAAGA
    CTACTCAAAG TGTTCCAAGG AGGAAAAGAA GTACAGGATG CTAACAGATT CACCAGCCAC
    CTACAGATAG AGAACACTTA CTCAGAGAAC TTCGTAAAAG TATTTCAAGA CCTAGGTGCC
    AAGGATCTGG AGCCAGTTGA AGTTGCTGTT CTCTTGAAGC ATCCTTTTAT TCAAGACCTG
    ATTTCAAATT ATTCAGACTA TAAAATCCCT GATATTAAAA TAATTCTCCA AAGGAGTGAG
    CATGTACAAG GAAGTGATGG AGAAAGATCT CCTTCAAGAC TTACAGAGGA AAAGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968621.1
    CDS159..5378
    Translation

    Target ORF information:

    RefSeq Version XM_014113146.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sperm flagellar 2 (SPEF2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113146.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCGGAGA TTCTGTGCCA GTGGCTCAAC CAGGAGGTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAAT TCTGCACAAG
    TTTGAACTTC AGGGTGATTT TTCGGAATTT TCAGAAAGCA GAGTTTCAAA TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCGACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCACA ACATCATAAC GGAAAAGCCT GGGGCGGCAA CCAAACTCTT GTATCAGTTG
    TACATTGTTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAATAGAAAT GCAAACTATG
    CAACCCCTGG CAAACGTCAG ATACCAAAGT ATGAAAAGTG AGGCTTTTCG AGATCAACGC
    CTGAGCAGGA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCTG CATCAAATCG TACTTTGAGA GCACTGGAGG CCCAAAAAAT GATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAG ATTAAGAAGT TTGAAGCTTT AATAAAAAAG
    GATCTCCAGG CCAAAGAAAG TGCCTCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACT
    ACTGATTTGT TAAACACTTA TTCGGATGAT GACTACATTA AAAAAATCCA AAAGCGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT ATTAATGGAC
    CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCGTATCGGG AGGAGCAGCT GATTAACCGG
    CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATCTCTGTGC AGCTCATGCA TGTTCGGCAT
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCCGAGAAA AACAGTATGA GGAAAGACGA
    CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT
    GATTTTCAGG AACAAGCCAT TAGAGAGAAG GAAATTCATG AACAGATCAC TGTGGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATACA ATATGTGCAG AAATTTTGGA TCAAATACTT
    GATCTTTCCA CTAAAGTGTC AGACTACCGG ATGTTGACAA ATAATTTGAT TCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT AATGGAAAGC CCATATACAA GCAAGCCTCT
    ATTAAGCAAC TACCAGCCAC GCCCTCTGCA GAACAAGTTA TAGAACTGGA GAAGAGGGAC
    TTGCTGGATA GCAATGATTA TGAAGAATAT AAGAATATGG TTGGAGAGTG GGCTTTACCA
    GAAGAAATGG TTGATAATTT ACCACCCTCC AACAATACCA TATTGGGTCA TGTGATTCAC
    AGATTAATTG AAAAATCTCA TCCTCCTCAA GTCAAATCAA CAGTACTAGA ATTACCTTCA
    TTTGCTATTA AAGGGTGCTT AGTTGGAAAA ACATTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTACAAA AAGACTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT CCAAGAAGCA
    ATCAAAGCAT TCCATGACAA TGAAAAAATC AGTGAGGCTC TACCAATTGA GAAAGAAGCT
    GAAGAAAAGT CTCTACCAGT TCTGCAAGAG AAGAACAAAG AAAGCCAGGA TCTACAGAAT
    GCATTTTCAC CTCATCCAGT TTCAGAAGAA GCGTTACCAG AAACAGAAGA TAAAACTATA
    CTTAGTACTG ATACAAATAA AACTCCAAAA GCTGAAGAAG TCAAATCAAG TGATAGTTTC
    TCTGAACTGA CTGTGCGGGC TCAGCTTGGT GCAAAATCAG AAAAGATGCT GAAGAAAGGA
    AAGAGCATTT CTGACATGTT GCTAGTTAAT ATCTTGGTAA ATGCTATTAA TGAGGTACCT
    GTGGATCAAG GCTGGGTCTT GGATGGTTTT CCAATGACGT TAAACCAAGC AAAGCTTCTG
    GAGGAAGCTC TCACGGGCTA CAACAGAGAG GCCCTAGAAC TGGAAGAAAA AAAAACACAA
    ATACCCACAT TGGCTGTTGA TGCTAGCACC TCCAAAGAAG TGCCTCTTCC CCCTTCTGCA
    TTTGACTTTG TCATGTTATT AGATATTTCA GATAATTCCT TGCTGGACCG CATGAATTAC
    ACAATGGCTG AAGCATTTTC AAGTGAAACT TCTCATGAAG ACATCACTCA ACGTGTAACT
    GCTGAAAAGC AAGATATGAA TGAGGACCAG AATTTAAGAG ACCAGATTCA GCACAGAATT
    ATAGGCTTCT TGGACAACTG GCCTTTGTTG GAGCAATGGT TTACAGAGCC AGAAAATATT
    TTGATAAATA TCAATGCTGA AGTAGATAAA GAGTCTTTAT GCAATAAAGT AAAAGAAATG
    TTTATGACTG AAATAATGAA AAAAGAGAAT AAAGTTAAAA AGAAATTAGA AGAAAAGGAA
    GCTGAGAAAA AAGAAGTTCC CCTGGGTGAG CCTCCGCCTC CTCCTCCTCC CCCTCCCCCT
    CCTCCTCCTG AGCCAGAAAA AGAGAAGGAA ACCCATCCTC AACGAGAGCA TTCAAAAACT
    CCTACTGCAA AAGGGAAACC ACCAGCAGGT TCTCCTAAAG GAAAATCACC AGGAGGGAAA
    GGACCACTAA AGAAATCACC TGCTGATTCT ACAGACATGT CACCTGTTCC CATAGTGCCA
    CCACCACCCA AGCCAGGATC AGAAGAATGG GTCTACGTGA ATGAACCAAT TCCTGAGGAA
    ATGCCTTCAT TTTTAGTGCC TTACTGGGAA CTAATCGAAA ATTCCTACAT AAACTCCATC
    AAAACAGTAC TCAGGCATCT GAGAGAAGAA CAGCATAGTG TGGTTGCTTA CTTATACGAG
    GTTAGAACAA ATTTCCAGCA GTTTCTATGG CGTCCAGATC ACAAGCAAGA TTTTGTATCT
    CAATGGCAGG CTGATTTCAA TGCTCTTCCT GATGACCTGT GGGATGATGA AGAAACAAAG
    TCTGAACTAC ACCAAAGAGT AAATGATTTA AGAGACCGCC TGTGGGACAT CTGTGATGCC
    CGGAAGGAAG AAGCAGAGCA GGAGCGTCAG GATATCATTA ACGAGAGCTG GTTGCAAGAC
    TCAATCGGAA TAACGATGAA CCATTTCTTC TCACTAATGC AGACAGAACT GAACCGTTTC
    CAAGATACAA AGAGACTCCT TCAAGATTAT TACAGGGGAA TGGAATGCAA AATTCCAATC
    GATGACAGTA AGAGATTTAC TCGAATCCCG TTGGTCCAGC TGGATAGTAG TAGAGACATT
    TTGGAAAGCC AACTCAGAAT TCCTCTAATT CCACGAATAT CCATTTCCCC GGATTCCGCT
    ATGACCAAAC CAAAAACAAA GGCCATACTA AAGGGCAAGA TCGATTACTC GCTGGAAAAT
    GTGGAGTTAA ACTTTGAGGC AGATGAGAAG TTGGTGATGG ACACCTGGCA GCAGGCTTCT
    TTAGCAATAT CTCACATGGT GGCTGCTGAA ATTCATCAGA GGCTCATGGA AGAAGAAAAG
    GAAAACCAAC CAGCAGACAC AAAAGAAAAA TCTCCTCAGA TGGGGACAAA TAAAAAAGCC
    AAAGAGGAAC CACCCAAGAA AGAAAAGGCA GACAAAAAAG CAAAAGGCAG ATCCCCGCCG
    ACAGCAGAAG CCACGCCTGT GACAGTCACA GCAGAGGAGA TTACCCAAAA GGAGAAGAGA
    AACGAACTGA TGCTCAAAAT CAAGGAAGAG CACCTCGCTG CCCTGCAGTT TGAAGAGATA
    GCCACACAGT TCCGGCTTGA ACTGATAAAG ACAAAAGCAT TGGCTTTCCT TGAAGATTTA
    CTAACAAAGG TGGTCGATGT ATATAAACTC TTGGAAAAGT GGCTTGGTGA GAGGTACTTG
    AATGAAATGG CCAGTGTGGA AAAATTAACT GAGGTGGCCC GTTATCACAT TGAAACATCA
    ACAAAAATTC AGAATGAACT TTACCTAGAC CAAGAAGATT TCTTCATTAA TGGAGATCTG
    AAAGTCTTTC CAGATCCCCC TCCCCCAGTG CGCCCCCCAC CTGTGGAAAA GGAAGAAAAT
    GGCACCCTGA CCATCGAACA GCTTGACAGT CTTAAAGATC AGTTCTTAGA TATTGCACCC
    AAAGGCATAA TAGGACATAA AGCATTTACT GACATTCTGC TGGATCTGGT AACCCTGAAC
    CTTGGAACAA ACAACTTACC TAGTAGTTGG ATGCACCTCA CGCAACCAGA ATTGCAGGAG
    TTAACATCTT TAGTAATAGT AAATTCGGAG TTTGTGGACT GGCGGAAATT CCTATTAGTA
    ATCTCGATGC CTTGGCCCAT CCCCATGGAG GAAGAGCTCC TCGAAACCCT GCAGAGGTTC
    AAGGCGGTGG ATGAAGAGCA GTTGGGCACC ATCACTTTTG AGCAGTATAT GCAGGCTGGT
    CTGTGGTTTA CAGGAGATGA ATATATAAAA ATTCCAGAAA ATCCTCTTGA ACCTCTGCCG
    TTCAATAGGC AGGAGCATCT TATAGAGTTT TTCTTTAGGC TATTTGCTGA CCATGAGAAG
    GATCCACCTC AACTTGACTA CACACAGATG CTGCTCTATT TTGCTTGCCA TCCAGATGCT
    ATGGAAGGGG TCTACAGGGC CCTCAGTGTG GCCATAGGAA CTCATGTCTT CCAACCAGTC
    GAAACTATTG CTATTATTGG AGAAAAGTCC TCCTCCTTGA CTGATGCAAG TCCAGTTGAG
    GAGTATCCTG AACTCGAGGG AAACTTTATG CATGAGGAAG GAGAATTAAA AGAAGACAGG
    GAGGAAAAGG AAGTAGAAAT CCCTGAGAAT GCAAACACTG AAAAGGTATC CCTGGAAAGA
    CTACTCAAAG TGTTCCAAGG AGGAAAAGAA GTACAGGATG CTAACAGATT CACCAGCCAC
    CTACAGATAG AGAACACTTA CTCAGAGAAC TTCGTAAAAG TATTTCAAGA CCTAGGTGCC
    AAGGATCTGG AGCCAGTTGA AGTTGCTGTT CTCTTGAAGC ATCCTTTTAT TCAAGACCTG
    ATTTCAAATT ATTCAGACTA TAAAATCCCT GATATTAAAA TAATTCTCCA AAGGAGTGAG
    CATGTACAAG GAAGTGATGG AGAAAGATCT CCTTCAAGAC TTACAGAGGA AAAGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36165
    Clone ID Related Accession (Same CDS sequence) XM_014113146.1 , XM_014113146.2
    Accession Version XM_014113146.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5220bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product sperm flagellar protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113146.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCGGAGA TTCTGTGCCA GTGGCTCAAC CAGGAGGTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAAT TCTGCACAAG
    TTTGAACTTC AGGGTGATTT TTCGGAATTT TCAGAAAGCA GAGTTTCAAA TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCGACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCACA ACATCATAAC GGAAAAGCCT GGGGCGGCAA CCAAACTCTT GTATCAGTTG
    TACATTGTTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAATAGAAAT GCAAACTATG
    CAACCCCTGG CAAACGTCAG ATACCAAAGT ATGAAAAGTG AGGCTTTTCG AGATCAACGC
    CTGAGCAGGA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCTG CATCAAATCG TACTTTGAGA GCACTGGAGG CCCAAAAAAT GATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAG ATTAAGAAGT TTGAAGCTTT AATAAAAAAG
    GATCTCCAGG CCAAAGAAAG TGCCTCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACT
    ACTGATTTGT TAAACACTTA TTCGGATGAT GACTACATTA AAAAAATCCA AAAGCGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT ATTAATGGAC
    CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCGTATCGGG AGGAGCAGCT GATTAACCGG
    CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATCTCTGTGC AGCTCATGCA TGTTCGGCAT
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCCGAGAAA AACAGTATGA GGAAAGACGA
    CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT
    GATTTTCAGG AACAAGCCAT TAGAGAGAAG GAAATTCATG AACAGATCAC TGTGGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATACA ATATGTGCAG AAATTTTGGA TCAAATACTT
    GATCTTTCCA CTAAAGTGTC AGACTACCGG ATGTTGACAA ATAATTTGAT TCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT AATGGAAAGC CCATATACAA GCAAGCCTCT
    ATTAAGCAAC TACCAGCCAC GCCCTCTGCA GAACAAGTTA TAGAACTGGA GAAGAGGGAC
    TTGCTGGATA GCAATGATTA TGAAGAATAT AAGAATATGG TTGGAGAGTG GGCTTTACCA
    GAAGAAATGG TTGATAATTT ACCACCCTCC AACAATACCA TATTGGGTCA TGTGATTCAC
    AGATTAATTG AAAAATCTCA TCCTCCTCAA GTCAAATCAA CAGTACTAGA ATTACCTTCA
    TTTGCTATTA AAGGGTGCTT AGTTGGAAAA ACATTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTACAAA AAGACTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT CCAAGAAGCA
    ATCAAAGCAT TCCATGACAA TGAAAAAATC AGTGAGGCTC TACCAATTGA GAAAGAAGCT
    GAAGAAAAGT CTCTACCAGT TCTGCAAGAG AAGAACAAAG AAAGCCAGGA TCTACAGAAT
    GCATTTTCAC CTCATCCAGT TTCAGAAGAA GCGTTACCAG AAACAGAAGA TAAAACTATA
    CTTAGTACTG ATACAAATAA AACTCCAAAA GCTGAAGAAG TCAAATCAAG TGATAGTTTC
    TCTGAACTGA CTGTGCGGGC TCAGCTTGGT GCAAAATCAG AAAAGATGCT GAAGAAAGGA
    AAGAGCATTT CTGACATGTT GCTAGTTAAT ATCTTGGTAA ATGCTATTAA TGAGGTACCT
    GTGGATCAAG GCTGGGTCTT GGATGGTTTT CCAATGACGT TAAACCAAGC AAAGCTTCTG
    GAGGAAGCTC TCACGGGCTA CAACAGAGAG GCCCTAGAAC TGGAAGAAAA AAAAACACAA
    ATACCCACAT TGGCTGTTGA TGCTAGCACC TCCAAAGAAG TGCCTCTTCC CCCTTCTGCA
    TTTGACTTTG TCATGTTATT AGATATTTCA GATAATTCCT TGCTGGACCG CATGAATTAC
    ACAATGGCTG AAGCATTTTC AAGTGAAACT TCTCATGAAG ACATCACTCA ACGTGTAACT
    GCTGAAAAGC AAGATATGAA TGAGGACCAG AATTTAAGAG ACCAGATTCA GCACAGAATT
    ATAGGCTTCT TGGACAACTG GCCTTTGTTG GAGCAATGGT TTACAGAGCC AGAAAATATT
    TTGATAAATA TCAATGCTGA AGTAGATAAA GAGTCTTTAT GCAATAAAGT AAAAGAAATG
    TTTATGACTG AAATAATGAA AAAAGAGAAT AAAGTTAAAA AGAAATTAGA AGAAAAGGAA
    GCTGAGAAAA AAGAAGTTCC CCTGGGTGAG CCTCCGCCTC CTCCTCCTCC CCCTCCCCCT
    CCTCCTCCTG AGCCAGAAAA AGAGAAGGAA ACCCATCCTC AACGAGAGCA TTCAAAAACT
    CCTACTGCAA AAGGGAAACC ACCAGCAGGT TCTCCTAAAG GAAAATCACC AGGAGGGAAA
    GGACCACTAA AGAAATCACC TGCTGATTCT ACAGACATGT CACCTGTTCC CATAGTGCCA
    CCACCACCCA AGCCAGGATC AGAAGAATGG GTCTACGTGA ATGAACCAAT TCCTGAGGAA
    ATGCCTTCAT TTTTAGTGCC TTACTGGGAA CTAATCGAAA ATTCCTACAT AAACTCCATC
    AAAACAGTAC TCAGGCATCT GAGAGAAGAA CAGCATAGTG TGGTTGCTTA CTTATACGAG
    GTTAGAACAA ATTTCCAGCA GTTTCTATGG CGTCCAGATC ACAAGCAAGA TTTTGTATCT
    CAATGGCAGG CTGATTTCAA TGCTCTTCCT GATGACCTGT GGGATGATGA AGAAACAAAG
    TCTGAACTAC ACCAAAGAGT AAATGATTTA AGAGACCGCC TGTGGGACAT CTGTGATGCC
    CGGAAGGAAG AAGCAGAGCA GGAGCGTCAG GATATCATTA ACGAGAGCTG GTTGCAAGAC
    TCAATCGGAA TAACGATGAA CCATTTCTTC TCACTAATGC AGACAGAACT GAACCGTTTC
    CAAGATACAA AGAGACTCCT TCAAGATTAT TACAGGGGAA TGGAATGCAA AATTCCAATC
    GATGACAGTA AGAGATTTAC TCGAATCCCG TTGGTCCAGC TGGATAGTAG TAGAGACATT
    TTGGAAAGCC AACTCAGAAT TCCTCTAATT CCACGAATAT CCATTTCCCC GGATTCCGCT
    ATGACCAAAC CAAAAACAAA GGCCATACTA AAGGGCAAGA TCGATTACTC GCTGGAAAAT
    GTGGAGTTAA ACTTTGAGGC AGATGAGAAG TTGGTGATGG ACACCTGGCA GCAGGCTTCT
    TTAGCAATAT CTCACATGGT GGCTGCTGAA ATTCATCAGA GGCTCATGGA AGAAGAAAAG
    GAAAACCAAC CAGCAGACAC AAAAGAAAAA TCTCCTCAGA TGGGGACAAA TAAAAAAGCC
    AAAGAGGAAC CACCCAAGAA AGAAAAGGCA GACAAAAAAG CAAAAGGCAG ATCCCCGCCG
    ACAGCAGAAG CCACGCCTGT GACAGTCACA GCAGAGGAGA TTACCCAAAA GGAGAAGAGA
    AACGAACTGA TGCTCAAAAT CAAGGAAGAG CACCTCGCTG CCCTGCAGTT TGAAGAGATA
    GCCACACAGT TCCGGCTTGA ACTGATAAAG ACAAAAGCAT TGGCTTTCCT TGAAGATTTA
    CTAACAAAGG TGGTCGATGT ATATAAACTC TTGGAAAAGT GGCTTGGTGA GAGGTACTTG
    AATGAAATGG CCAGTGTGGA AAAATTAACT GAGGTGGCCC GTTATCACAT TGAAACATCA
    ACAAAAATTC AGAATGAACT TTACCTAGAC CAAGAAGATT TCTTCATTAA TGGAGATCTG
    AAAGTCTTTC CAGATCCCCC TCCCCCAGTG CGCCCCCCAC CTGTGGAAAA GGAAGAAAAT
    GGCACCCTGA CCATCGAACA GCTTGACAGT CTTAAAGATC AGTTCTTAGA TATTGCACCC
    AAAGGCATAA TAGGACATAA AGCATTTACT GACATTCTGC TGGATCTGGT AACCCTGAAC
    CTTGGAACAA ACAACTTACC TAGTAGTTGG ATGCACCTCA CGCAACCAGA ATTGCAGGAG
    TTAACATCTT TAGTAATAGT AAATTCGGAG TTTGTGGACT GGCGGAAATT CCTATTAGTA
    ATCTCGATGC CTTGGCCCAT CCCCATGGAG GAAGAGCTCC TCGAAACCCT GCAGAGGTTC
    AAGGCGGTGG ATGAAGAGCA GTTGGGCACC ATCACTTTTG AGCAGTATAT GCAGGCTGGT
    CTGTGGTTTA CAGGAGATGA ATATATAAAA ATTCCAGAAA ATCCTCTTGA ACCTCTGCCG
    TTCAATAGGC AGGAGCATCT TATAGAGTTT TTCTTTAGGC TATTTGCTGA CCATGAGAAG
    GATCCACCTC AACTTGACTA CACACAGATG CTGCTCTATT TTGCTTGCCA TCCAGATGCT
    ATGGAAGGGG TCTACAGGGC CCTCAGTGTG GCCATAGGAA CTCATGTCTT CCAACCAGTC
    GAAACTATTG CTATTATTGG AGAAAAGTCC TCCTCCTTGA CTGATGCAAG TCCAGTTGAG
    GAGTATCCTG AACTCGAGGG AAACTTTATG CATGAGGAAG GAGAATTAAA AGAAGACAGG
    GAGGAAAAGG AAGTAGAAAT CCCTGAGAAT GCAAACACTG AAAAGGTATC CCTGGAAAGA
    CTACTCAAAG TGTTCCAAGG AGGAAAAGAA GTACAGGATG CTAACAGATT CACCAGCCAC
    CTACAGATAG AGAACACTTA CTCAGAGAAC TTCGTAAAAG TATTTCAAGA CCTAGGTGCC
    AAGGATCTGG AGCCAGTTGA AGTTGCTGTT CTCTTGAAGC ATCCTTTTAT TCAAGACCTG
    ATTTCAAATT ATTCAGACTA TAAAATCCCT GATATTAAAA TAATTCTCCA AAGGAGTGAG
    CATGTACAAG GAAGTGATGG AGAAAGATCT CCTTCAAGAC TTACAGAGGA AAAGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968621.1
    CDS159..5378
    Translation

    Target ORF information:

    RefSeq Version XM_014113146.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris sperm flagellar protein 2 (LOC489235), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113146.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCGGAGA TTCTGTGCCA GTGGCTCAAC CAGGAGGTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAAT TCTGCACAAG
    TTTGAACTTC AGGGTGATTT TTCGGAATTT TCAGAAAGCA GAGTTTCAAA TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCGACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCACA ACATCATAAC GGAAAAGCCT GGGGCGGCAA CCAAACTCTT GTATCAGTTG
    TACATTGTTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAATAGAAAT GCAAACTATG
    CAACCCCTGG CAAACGTCAG ATACCAAAGT ATGAAAAGTG AGGCTTTTCG AGATCAACGC
    CTGAGCAGGA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCTG CATCAAATCG TACTTTGAGA GCACTGGAGG CCCAAAAAAT GATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAG ATTAAGAAGT TTGAAGCTTT AATAAAAAAG
    GATCTCCAGG CCAAAGAAAG TGCCTCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACT
    ACTGATTTGT TAAACACTTA TTCGGATGAT GACTACATTA AAAAAATCCA AAAGCGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT ATTAATGGAC
    CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCGTATCGGG AGGAGCAGCT GATTAACCGG
    CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATCTCTGTGC AGCTCATGCA TGTTCGGCAT
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCCGAGAAA AACAGTATGA GGAAAGACGA
    CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT
    GATTTTCAGG AACAAGCCAT TAGAGAGAAG GAAATTCATG AACAGATCAC TGTGGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATACA ATATGTGCAG AAATTTTGGA TCAAATACTT
    GATCTTTCCA CTAAAGTGTC AGACTACCGG ATGTTGACAA ATAATTTGAT TCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT AATGGAAAGC CCATATACAA GCAAGCCTCT
    ATTAAGCAAC TACCAGCCAC GCCCTCTGCA GAACAAGTTA TAGAACTGGA GAAGAGGGAC
    TTGCTGGATA GCAATGATTA TGAAGAATAT AAGAATATGG TTGGAGAGTG GGCTTTACCA
    GAAGAAATGG TTGATAATTT ACCACCCTCC AACAATACCA TATTGGGTCA TGTGATTCAC
    AGATTAATTG AAAAATCTCA TCCTCCTCAA GTCAAATCAA CAGTACTAGA ATTACCTTCA
    TTTGCTATTA AAGGGTGCTT AGTTGGAAAA ACATTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTACAAA AAGACTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT CCAAGAAGCA
    ATCAAAGCAT TCCATGACAA TGAAAAAATC AGTGAGGCTC TACCAATTGA GAAAGAAGCT
    GAAGAAAAGT CTCTACCAGT TCTGCAAGAG AAGAACAAAG AAAGCCAGGA TCTACAGAAT
    GCATTTTCAC CTCATCCAGT TTCAGAAGAA GCGTTACCAG AAACAGAAGA TAAAACTATA
    CTTAGTACTG ATACAAATAA AACTCCAAAA GCTGAAGAAG TCAAATCAAG TGATAGTTTC
    TCTGAACTGA CTGTGCGGGC TCAGCTTGGT GCAAAATCAG AAAAGATGCT GAAGAAAGGA
    AAGAGCATTT CTGACATGTT GCTAGTTAAT ATCTTGGTAA ATGCTATTAA TGAGGTACCT
    GTGGATCAAG GCTGGGTCTT GGATGGTTTT CCAATGACGT TAAACCAAGC AAAGCTTCTG
    GAGGAAGCTC TCACGGGCTA CAACAGAGAG GCCCTAGAAC TGGAAGAAAA AAAAACACAA
    ATACCCACAT TGGCTGTTGA TGCTAGCACC TCCAAAGAAG TGCCTCTTCC CCCTTCTGCA
    TTTGACTTTG TCATGTTATT AGATATTTCA GATAATTCCT TGCTGGACCG CATGAATTAC
    ACAATGGCTG AAGCATTTTC AAGTGAAACT TCTCATGAAG ACATCACTCA ACGTGTAACT
    GCTGAAAAGC AAGATATGAA TGAGGACCAG AATTTAAGAG ACCAGATTCA GCACAGAATT
    ATAGGCTTCT TGGACAACTG GCCTTTGTTG GAGCAATGGT TTACAGAGCC AGAAAATATT
    TTGATAAATA TCAATGCTGA AGTAGATAAA GAGTCTTTAT GCAATAAAGT AAAAGAAATG
    TTTATGACTG AAATAATGAA AAAAGAGAAT AAAGTTAAAA AGAAATTAGA AGAAAAGGAA
    GCTGAGAAAA AAGAAGTTCC CCTGGGTGAG CCTCCGCCTC CTCCTCCTCC CCCTCCCCCT
    CCTCCTCCTG AGCCAGAAAA AGAGAAGGAA ACCCATCCTC AACGAGAGCA TTCAAAAACT
    CCTACTGCAA AAGGGAAACC ACCAGCAGGT TCTCCTAAAG GAAAATCACC AGGAGGGAAA
    GGACCACTAA AGAAATCACC TGCTGATTCT ACAGACATGT CACCTGTTCC CATAGTGCCA
    CCACCACCCA AGCCAGGATC AGAAGAATGG GTCTACGTGA ATGAACCAAT TCCTGAGGAA
    ATGCCTTCAT TTTTAGTGCC TTACTGGGAA CTAATCGAAA ATTCCTACAT AAACTCCATC
    AAAACAGTAC TCAGGCATCT GAGAGAAGAA CAGCATAGTG TGGTTGCTTA CTTATACGAG
    GTTAGAACAA ATTTCCAGCA GTTTCTATGG CGTCCAGATC ACAAGCAAGA TTTTGTATCT
    CAATGGCAGG CTGATTTCAA TGCTCTTCCT GATGACCTGT GGGATGATGA AGAAACAAAG
    TCTGAACTAC ACCAAAGAGT AAATGATTTA AGAGACCGCC TGTGGGACAT CTGTGATGCC
    CGGAAGGAAG AAGCAGAGCA GGAGCGTCAG GATATCATTA ACGAGAGCTG GTTGCAAGAC
    TCAATCGGAA TAACGATGAA CCATTTCTTC TCACTAATGC AGACAGAACT GAACCGTTTC
    CAAGATACAA AGAGACTCCT TCAAGATTAT TACAGGGGAA TGGAATGCAA AATTCCAATC
    GATGACAGTA AGAGATTTAC TCGAATCCCG TTGGTCCAGC TGGATAGTAG TAGAGACATT
    TTGGAAAGCC AACTCAGAAT TCCTCTAATT CCACGAATAT CCATTTCCCC GGATTCCGCT
    ATGACCAAAC CAAAAACAAA GGCCATACTA AAGGGCAAGA TCGATTACTC GCTGGAAAAT
    GTGGAGTTAA ACTTTGAGGC AGATGAGAAG TTGGTGATGG ACACCTGGCA GCAGGCTTCT
    TTAGCAATAT CTCACATGGT GGCTGCTGAA ATTCATCAGA GGCTCATGGA AGAAGAAAAG
    GAAAACCAAC CAGCAGACAC AAAAGAAAAA TCTCCTCAGA TGGGGACAAA TAAAAAAGCC
    AAAGAGGAAC CACCCAAGAA AGAAAAGGCA GACAAAAAAG CAAAAGGCAG ATCCCCGCCG
    ACAGCAGAAG CCACGCCTGT GACAGTCACA GCAGAGGAGA TTACCCAAAA GGAGAAGAGA
    AACGAACTGA TGCTCAAAAT CAAGGAAGAG CACCTCGCTG CCCTGCAGTT TGAAGAGATA
    GCCACACAGT TCCGGCTTGA ACTGATAAAG ACAAAAGCAT TGGCTTTCCT TGAAGATTTA
    CTAACAAAGG TGGTCGATGT ATATAAACTC TTGGAAAAGT GGCTTGGTGA GAGGTACTTG
    AATGAAATGG CCAGTGTGGA AAAATTAACT GAGGTGGCCC GTTATCACAT TGAAACATCA
    ACAAAAATTC AGAATGAACT TTACCTAGAC CAAGAAGATT TCTTCATTAA TGGAGATCTG
    AAAGTCTTTC CAGATCCCCC TCCCCCAGTG CGCCCCCCAC CTGTGGAAAA GGAAGAAAAT
    GGCACCCTGA CCATCGAACA GCTTGACAGT CTTAAAGATC AGTTCTTAGA TATTGCACCC
    AAAGGCATAA TAGGACATAA AGCATTTACT GACATTCTGC TGGATCTGGT AACCCTGAAC
    CTTGGAACAA ACAACTTACC TAGTAGTTGG ATGCACCTCA CGCAACCAGA ATTGCAGGAG
    TTAACATCTT TAGTAATAGT AAATTCGGAG TTTGTGGACT GGCGGAAATT CCTATTAGTA
    ATCTCGATGC CTTGGCCCAT CCCCATGGAG GAAGAGCTCC TCGAAACCCT GCAGAGGTTC
    AAGGCGGTGG ATGAAGAGCA GTTGGGCACC ATCACTTTTG AGCAGTATAT GCAGGCTGGT
    CTGTGGTTTA CAGGAGATGA ATATATAAAA ATTCCAGAAA ATCCTCTTGA ACCTCTGCCG
    TTCAATAGGC AGGAGCATCT TATAGAGTTT TTCTTTAGGC TATTTGCTGA CCATGAGAAG
    GATCCACCTC AACTTGACTA CACACAGATG CTGCTCTATT TTGCTTGCCA TCCAGATGCT
    ATGGAAGGGG TCTACAGGGC CCTCAGTGTG GCCATAGGAA CTCATGTCTT CCAACCAGTC
    GAAACTATTG CTATTATTGG AGAAAAGTCC TCCTCCTTGA CTGATGCAAG TCCAGTTGAG
    GAGTATCCTG AACTCGAGGG AAACTTTATG CATGAGGAAG GAGAATTAAA AGAAGACAGG
    GAGGAAAAGG AAGTAGAAAT CCCTGAGAAT GCAAACACTG AAAAGGTATC CCTGGAAAGA
    CTACTCAAAG TGTTCCAAGG AGGAAAAGAA GTACAGGATG CTAACAGATT CACCAGCCAC
    CTACAGATAG AGAACACTTA CTCAGAGAAC TTCGTAAAAG TATTTCAAGA CCTAGGTGCC
    AAGGATCTGG AGCCAGTTGA AGTTGCTGTT CTCTTGAAGC ATCCTTTTAT TCAAGACCTG
    ATTTCAAATT ATTCAGACTA TAAAATCCCT GATATTAAAA TAATTCTCCA AAGGAGTGAG
    CATGTACAAG GAAGTGATGG AGAAAGATCT CCTTCAAGAC TTACAGAGGA AAAGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05532
    Clone ID Related Accession (Same CDS sequence) XM_546353.3 , XM_546353.4
    Accession Version XM_546353.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5466bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product sperm flagellar protein 2 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_546353.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGAGA TTCTGTGCCA GTGGCTCAAC CAGGAGGTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAAT TCTGCACAAG
    TTTGAACTTC AGGGTGATTT TTCGGAATTT TCAGAAAGCA GAGTTTCAAA TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCGACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCACA ACATCATAAC GGAAAAGCCT GGGGCGGCAA CCAAACTCTT GTATCAGTTG
    TACATTGTTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAATAGAAAT GCAAACTATG
    CAACCCCTGG CAAACGTCAG ATACCAAAGT ATGAAAAGTG AGGCTTTTCG AGATAGACTT
    AGAAACCTGA TACCACGTCA AACTGATTTC AATCTGATGC AGGTTACACA CAGGTTTCAA
    GAAAAATATA AACACATGAA AGAAGATCTT GTCCACATGC ATTTTGAGAA ACTTGAAAAA
    TTTCAAAAAC TCAAGGAAGA ACAAAGATGT TTTAATATTG AAAAGCAACG CCTGAGCAGG
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TCATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAG AGCACTGGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAGGATG TGGCCAATGA GATTAAGAAG TTTGAAGCTT TAATAAAAAA GGATCTCCAG
    GCCAAAGAAA GTGCCTCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC TACTGATTTG
    TTAAACACTT ATTCGGATGA TGACTACATT AAAAAAATCC AAAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAGAC TATTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCGTATCGG GAGGAGCAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATCTCTGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTGTGGC AAAACAGAAT TTTCCGAGAA AAACAGTATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTCAG
    GAACAAGCCA TTAGAGAGAA GGAAATTCAT GAACAGATCA CTGTGGAAAG AGCTCAAGCT
    CGTTACAAAA AGCATTATAC AATATGTGCA GAAATTTTGG ATCAAATACT TGATCTTTCC
    ACTAAAGTGT CAGACTACCG GATGTTGACA AATAATTTGA TTCCATATAA GTTGATGCAT
    GATTGGAAGG AACTGTTTTT TAATGGAAAG CCCATATACA AGCAAGCCTC TATTAAGCAA
    CTACCAGCCA CGCCCTCTGC AGAACAAGTT ATAGAACTGG AGAAGAGGGA CTTGCTGGAT
    AGCAATGATT ATGAAGAATA TAAGAATATG GTTGGAGAGT GGGCTTTACC AGAAGAAATG
    GTTGATAATT TACCACCCTC CAACAATACC ATATTGGGTC ATGTGATTCA CAGATTAATT
    GAAAAATCTC ATCCTCCTCA AGTCAAATCA ACAGTACTAG AATTACCTTC ATTTGCTATT
    AAAGGGTGCT TAGTTGGAAA AACATTTAGT GGAAAAACTA CTGCTCTAAA GGCTCTACAA
    AAAGACTTTC CTATTCAGGT ACTTTCTATT GACACTCTTG TCCAAGAAGC AATCAAAGCA
    TTCCATGACA ATGAAAAAAT CAGTGAGGCT CTACCAATTG AGAAAGAAGC TGAAGAAAAG
    TCTCTACCAG TTCTGCAAGA GAAGAACAAA GAAAGCCAGG ATCTACAGAA TGCATTTTCA
    CCTCATCCAG TTTCAGAAGA AGCGTTACCA GAAACAGAAG ATAAAACTAT ACTTAGTACT
    GATACAAATA AAACTCCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTCTGAACTG
    ACTGTGCGGG CTCAGCTTGG TGCAAAATCA GAAAAGATGC TGAAGAAAGG AAAGAGCATT
    TCTGACATGT TGCTAGTTAA TATCTTGGTA AATGCTATTA ATGAGGTACC TGTGGATCAA
    GGCTGGGTCT TGGATGGTTT TCCAATGACG TTAAACCAAG CAAAGCTTCT GGAGGAAGCT
    CTCACGGGCT ACAACAGAGA GGCCCTAGAA CTGGAAGAAA AAAAAACACA AATACCCACA
    TTGGCTGTTG ATGCTAGCAC CTCCAAAGAA GTGCCTCTTC CCCCTTCTGC ATTTGACTTT
    GTCATGTTAT TAGATATTTC AGATAATTCC TTGCTGGACC GCATGAATTA CACAATGGCT
    GAAGCATTTT CAAGTGAAAC TTCTCATGAA GACATCACTC AACGTGTAAC TGCTGAAAAG
    CAAGATATGA ATGAGGACCA GAATTTAAGA GACCAGATTC AGCACAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTGTT GGAGCAATGG TTTACAGAGC CAGAAAATAT TTTGATAAAT
    ATCAATGCTG AAGTAGATAA AGAGTCTTTA TGCAATAAAG TAAAAGAAAT GTTTATGACT
    GAAATAATGA AAAAAGAGAA TAAAGTTAAA AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGAAGTTC CCCTGGGTGA GCCTCCGCCT CCTCCTCCTC CCCCTCCCCC TCCTCCTCCT
    GAGCCAGAAA AAGAGAAGGA AACCCATCCT CAACGAGAGC ATTCAAAAAC TCCTACTGCA
    AAAGGGAAAC CACCAGCAGA ACCCTTGACT GGTAAGCCAG ACTCTCTTCA GGAAGGAAAA
    GGAAAGAAAG GGGACACTTC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCACCAGGA
    GGGAAAGGAC CACTAAAGAA ATCACCTGCT GATTCTACAG ACATGTCACC TGTTCCCATA
    GTGCCACCAC CACCCAAGCC AGGATCAGAA GAATGGGTCT ACGTGAATGA ACCAATTCCT
    GAGGAAATGC CTTCATTTTT AGTGCCTTAC TGGGAACTAA TCGAAAATTC CTACATAAAC
    TCCATCAAAA CAGTACTCAG GCATCTGAGA GAAGAACAGC ATAGTGTGGT TGCTTACTTA
    TACGAGGTTA GAACAAATTT CCAGCAGTTT CTATGGCGTC CAGATCACAA GCAAGATTTT
    GTATCTCAAT GGCAGGCTGA TTTCAATGCT CTTCCTGATG ACCTGTGGGA TGATGAAGAA
    ACAAAGTCTG AACTACACCA AAGAGTAAAT GATTTAAGAG ACCGCCTGTG GGACATCTGT
    GATGCCCGGA AGGAAGAAGC AGAGCAGGAG CGTCAGGATA TCATTAACGA GAGCTGGTTG
    CAAGACTCAA TCGGAATAAC GATGAACCAT TTCTTCTCAC TAATGCAGAC AGAACTGAAC
    CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACA GGGGAATGGA ATGCAAAATT
    CCAATCGATG ACAGTAAGAG ATTTACTCGA ATCCCGTTGG TCCAGCTGGA TAGTAGTAGA
    GACATTTTGG AAAGCCAACT CAGAATTCCT CTAATTCCAC GAATATCCAT TTCCCCGGAT
    TCCGCTATGA CCAAACCAAA AACAAAGGCC ATACTAAAGG GCAAGATCGA TTACTCGCTG
    GAAAATGTGG AGTTAAACTT TGAGGCAGAT GAGAAGTTGG TGATGGACAC CTGGCAGCAG
    GCTTCTTTAG CAATATCTCA CATGGTGGCT GCTGAAATTC ATCAGAGGCT CATGGAAGAA
    GAAAAGGAAA ACCAACCAGC AGACACAAAA GAAAAATCTC CTCAGATGGG GACAAATAAA
    AAAGCCAAAG AGGAACCACC CAAGAAAGAA AAGGCAGACA AAAAAGCAAA AGGCAGATCC
    CCGCCGACAG CAGAAGCCAC GCCTGTGACA GTCACAGCAG AGGAGATTAC CCAAAAGGAG
    AAGAGAAACG AACTGATGCT CAAAATCAAG GAAGAGCACC TCGCTGCCCT GCAGTTTGAA
    GAGATAGCCA CACAGTTCCG GCTTGAACTG ATAAAGACAA AAGCATTGGC TTTCCTTGAA
    GATTTACTAA CAAAGGTGGT CGATGTATAT AAACTCTTGG AAAAGTGGCT TGGTGAGAGG
    TACTTGAATG AAATGGCCAG TGTGGAAAAA TTAACTGAGG TGGCCCGTTA TCACATTGAA
    ACATCAACAA AAATTCAGAA TGAACTTTAC CTAGACCAAG AAGATTTCTT CATTAATGGA
    GATCTGAAAG TCTTTCCAGA TCCCCCTCCC CCAGTGCGCC CCCCACCTGT GGAAAAGGAA
    GAAAATGGCA CCCTGACCAT CGAACAGCTT GACAGTCTTA AAGATCAGTT CTTAGATATT
    GCACCCAAAG GCATAATAGG ACATAAAGCA TTTACTGACA TTCTGCTGGA TCTGGTAACC
    CTGAACCTTG GAACAAACAA CTTACCTAGT AGTTGGATGC ACCTCACGCA ACCAGAATTG
    CAGGAGTTAA CATCTTTAGT AATAGTAAAT TCGGAGTTTG TGGACTGGCG GAAATTCCTA
    TTAGTAATCT CGATGCCTTG GCCCATCCCC ATGGAGGAAG AGCTCCTCGA AACCCTGCAG
    AGGTTCAAGG CGGTGGATGA AGAGCAGTTG GGCACCATCA CTTTTGAGCA GTATATGCAG
    GCTGGTCTGT GGTTTACAGG AGATGAATAT ATAAAAATTC CAGAAAATCC TCTTGAACCT
    CTGCCGTTCA ATAGGCAGGA GCATCTTATA GAGTTTTTCT TTAGGCTATT TGCTGACCAT
    GAGAAGGATC CACCTCAACT TGACTACACA CAGATGCTGC TCTATTTTGC TTGCCATCCA
    GATGCTATGG AAGGGGTCTA CAGGGCCCTC AGTGTGGCCA TAGGAACTCA TGTCTTCCAA
    CCAGTCGAAA CTATTGCTAT TATTGGAGAA AAGTCCTCCT CCTTGACTGA TGCAAGTCCA
    GTTGAGGAGT ATCCTGAACT CGAGGGAAAC TTTATGCATG AGGAAGGAGA ATTAAAAGAA
    GACAGGGAGG AAAAGGAAGT AGAAATCCCT GAGAATGCAA ACACTGAAAA GGTATCCCTG
    GAAAGACTAC TCAAAGTGTT CCAAGGAGGA AAAGAAGTAC AGGATGCTAA CAGATTCACC
    AGCCACCTAC AGATAGAGAA CACTTACTCA GAGAACTTCG TAAAAGTATT TCAAGACCTA
    GGTGCCAAGG ATCTGGAGCC AGTTGAAGTT GCTGTTCTCT TGAAGCATCC TTTTATTCAA
    GACCTGATTT CAAATTATTC AGACTATAAA ATCCCTGATA TTAAAATAAT TCTCCAAAGG
    AGTGAGCATG TACAAGGAAG TGATGGAGAA AGATCTCCTT CAAGACTTAC AGAGGAAAAG
    AAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_546353.2
    CDS159..5624
    Translation

    Target ORF information:

    RefSeq Version XM_546353.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sperm flagellar 2 (SPEF2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_546353.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGAGA TTCTGTGCCA GTGGCTCAAC CAGGAGGTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAAT TCTGCACAAG
    TTTGAACTTC AGGGTGATTT TTCGGAATTT TCAGAAAGCA GAGTTTCAAA TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCGACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCACA ACATCATAAC GGAAAAGCCT GGGGCGGCAA CCAAACTCTT GTATCAGTTG
    TACATTGTTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAATAGAAAT GCAAACTATG
    CAACCCCTGG CAAACGTCAG ATACCAAAGT ATGAAAAGTG AGGCTTTTCG AGATAGACTT
    AGAAACCTGA TACCACGTCA AACTGATTTC AATCTGATGC AGGTTACACA CAGGTTTCAA
    GAAAAATATA AACACATGAA AGAAGATCTT GTCCACATGC ATTTTGAGAA ACTTGAAAAA
    TTTCAAAAAC TCAAGGAAGA ACAAAGATGT TTTAATATTG AAAAGCAACG CCTGAGCAGG
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TCATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAG AGCACTGGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAGGATG TGGCCAATGA GATTAAGAAG TTTGAAGCTT TAATAAAAAA GGATCTCCAG
    GCCAAAGAAA GTGCCTCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC TACTGATTTG
    TTAAACACTT ATTCGGATGA TGACTACATT AAAAAAATCC AAAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAGAC TATTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCGTATCGG GAGGAGCAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATCTCTGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTGTGGC AAAACAGAAT TTTCCGAGAA AAACAGTATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTCAG
    GAACAAGCCA TTAGAGAGAA GGAAATTCAT GAACAGATCA CTGTGGAAAG AGCTCAAGCT
    CGTTACAAAA AGCATTATAC AATATGTGCA GAAATTTTGG ATCAAATACT TGATCTTTCC
    ACTAAAGTGT CAGACTACCG GATGTTGACA AATAATTTGA TTCCATATAA GTTGATGCAT
    GATTGGAAGG AACTGTTTTT TAATGGAAAG CCCATATACA AGCAAGCCTC TATTAAGCAA
    CTACCAGCCA CGCCCTCTGC AGAACAAGTT ATAGAACTGG AGAAGAGGGA CTTGCTGGAT
    AGCAATGATT ATGAAGAATA TAAGAATATG GTTGGAGAGT GGGCTTTACC AGAAGAAATG
    GTTGATAATT TACCACCCTC CAACAATACC ATATTGGGTC ATGTGATTCA CAGATTAATT
    GAAAAATCTC ATCCTCCTCA AGTCAAATCA ACAGTACTAG AATTACCTTC ATTTGCTATT
    AAAGGGTGCT TAGTTGGAAA AACATTTAGT GGAAAAACTA CTGCTCTAAA GGCTCTACAA
    AAAGACTTTC CTATTCAGGT ACTTTCTATT GACACTCTTG TCCAAGAAGC AATCAAAGCA
    TTCCATGACA ATGAAAAAAT CAGTGAGGCT CTACCAATTG AGAAAGAAGC TGAAGAAAAG
    TCTCTACCAG TTCTGCAAGA GAAGAACAAA GAAAGCCAGG ATCTACAGAA TGCATTTTCA
    CCTCATCCAG TTTCAGAAGA AGCGTTACCA GAAACAGAAG ATAAAACTAT ACTTAGTACT
    GATACAAATA AAACTCCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTCTGAACTG
    ACTGTGCGGG CTCAGCTTGG TGCAAAATCA GAAAAGATGC TGAAGAAAGG AAAGAGCATT
    TCTGACATGT TGCTAGTTAA TATCTTGGTA AATGCTATTA ATGAGGTACC TGTGGATCAA
    GGCTGGGTCT TGGATGGTTT TCCAATGACG TTAAACCAAG CAAAGCTTCT GGAGGAAGCT
    CTCACGGGCT ACAACAGAGA GGCCCTAGAA CTGGAAGAAA AAAAAACACA AATACCCACA
    TTGGCTGTTG ATGCTAGCAC CTCCAAAGAA GTGCCTCTTC CCCCTTCTGC ATTTGACTTT
    GTCATGTTAT TAGATATTTC AGATAATTCC TTGCTGGACC GCATGAATTA CACAATGGCT
    GAAGCATTTT CAAGTGAAAC TTCTCATGAA GACATCACTC AACGTGTAAC TGCTGAAAAG
    CAAGATATGA ATGAGGACCA GAATTTAAGA GACCAGATTC AGCACAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTGTT GGAGCAATGG TTTACAGAGC CAGAAAATAT TTTGATAAAT
    ATCAATGCTG AAGTAGATAA AGAGTCTTTA TGCAATAAAG TAAAAGAAAT GTTTATGACT
    GAAATAATGA AAAAAGAGAA TAAAGTTAAA AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGAAGTTC CCCTGGGTGA GCCTCCGCCT CCTCCTCCTC CCCCTCCCCC TCCTCCTCCT
    GAGCCAGAAA AAGAGAAGGA AACCCATCCT CAACGAGAGC ATTCAAAAAC TCCTACTGCA
    AAAGGGAAAC CACCAGCAGA ACCCTTGACT GGTAAGCCAG ACTCTCTTCA GGAAGGAAAA
    GGAAAGAAAG GGGACACTTC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCACCAGGA
    GGGAAAGGAC CACTAAAGAA ATCACCTGCT GATTCTACAG ACATGTCACC TGTTCCCATA
    GTGCCACCAC CACCCAAGCC AGGATCAGAA GAATGGGTCT ACGTGAATGA ACCAATTCCT
    GAGGAAATGC CTTCATTTTT AGTGCCTTAC TGGGAACTAA TCGAAAATTC CTACATAAAC
    TCCATCAAAA CAGTACTCAG GCATCTGAGA GAAGAACAGC ATAGTGTGGT TGCTTACTTA
    TACGAGGTTA GAACAAATTT CCAGCAGTTT CTATGGCGTC CAGATCACAA GCAAGATTTT
    GTATCTCAAT GGCAGGCTGA TTTCAATGCT CTTCCTGATG ACCTGTGGGA TGATGAAGAA
    ACAAAGTCTG AACTACACCA AAGAGTAAAT GATTTAAGAG ACCGCCTGTG GGACATCTGT
    GATGCCCGGA AGGAAGAAGC AGAGCAGGAG CGTCAGGATA TCATTAACGA GAGCTGGTTG
    CAAGACTCAA TCGGAATAAC GATGAACCAT TTCTTCTCAC TAATGCAGAC AGAACTGAAC
    CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACA GGGGAATGGA ATGCAAAATT
    CCAATCGATG ACAGTAAGAG ATTTACTCGA ATCCCGTTGG TCCAGCTGGA TAGTAGTAGA
    GACATTTTGG AAAGCCAACT CAGAATTCCT CTAATTCCAC GAATATCCAT TTCCCCGGAT
    TCCGCTATGA CCAAACCAAA AACAAAGGCC ATACTAAAGG GCAAGATCGA TTACTCGCTG
    GAAAATGTGG AGTTAAACTT TGAGGCAGAT GAGAAGTTGG TGATGGACAC CTGGCAGCAG
    GCTTCTTTAG CAATATCTCA CATGGTGGCT GCTGAAATTC ATCAGAGGCT CATGGAAGAA
    GAAAAGGAAA ACCAACCAGC AGACACAAAA GAAAAATCTC CTCAGATGGG GACAAATAAA
    AAAGCCAAAG AGGAACCACC CAAGAAAGAA AAGGCAGACA AAAAAGCAAA AGGCAGATCC
    CCGCCGACAG CAGAAGCCAC GCCTGTGACA GTCACAGCAG AGGAGATTAC CCAAAAGGAG
    AAGAGAAACG AACTGATGCT CAAAATCAAG GAAGAGCACC TCGCTGCCCT GCAGTTTGAA
    GAGATAGCCA CACAGTTCCG GCTTGAACTG ATAAAGACAA AAGCATTGGC TTTCCTTGAA
    GATTTACTAA CAAAGGTGGT CGATGTATAT AAACTCTTGG AAAAGTGGCT TGGTGAGAGG
    TACTTGAATG AAATGGCCAG TGTGGAAAAA TTAACTGAGG TGGCCCGTTA TCACATTGAA
    ACATCAACAA AAATTCAGAA TGAACTTTAC CTAGACCAAG AAGATTTCTT CATTAATGGA
    GATCTGAAAG TCTTTCCAGA TCCCCCTCCC CCAGTGCGCC CCCCACCTGT GGAAAAGGAA
    GAAAATGGCA CCCTGACCAT CGAACAGCTT GACAGTCTTA AAGATCAGTT CTTAGATATT
    GCACCCAAAG GCATAATAGG ACATAAAGCA TTTACTGACA TTCTGCTGGA TCTGGTAACC
    CTGAACCTTG GAACAAACAA CTTACCTAGT AGTTGGATGC ACCTCACGCA ACCAGAATTG
    CAGGAGTTAA CATCTTTAGT AATAGTAAAT TCGGAGTTTG TGGACTGGCG GAAATTCCTA
    TTAGTAATCT CGATGCCTTG GCCCATCCCC ATGGAGGAAG AGCTCCTCGA AACCCTGCAG
    AGGTTCAAGG CGGTGGATGA AGAGCAGTTG GGCACCATCA CTTTTGAGCA GTATATGCAG
    GCTGGTCTGT GGTTTACAGG AGATGAATAT ATAAAAATTC CAGAAAATCC TCTTGAACCT
    CTGCCGTTCA ATAGGCAGGA GCATCTTATA GAGTTTTTCT TTAGGCTATT TGCTGACCAT
    GAGAAGGATC CACCTCAACT TGACTACACA CAGATGCTGC TCTATTTTGC TTGCCATCCA
    GATGCTATGG AAGGGGTCTA CAGGGCCCTC AGTGTGGCCA TAGGAACTCA TGTCTTCCAA
    CCAGTCGAAA CTATTGCTAT TATTGGAGAA AAGTCCTCCT CCTTGACTGA TGCAAGTCCA
    GTTGAGGAGT ATCCTGAACT CGAGGGAAAC TTTATGCATG AGGAAGGAGA ATTAAAAGAA
    GACAGGGAGG AAAAGGAAGT AGAAATCCCT GAGAATGCAA ACACTGAAAA GGTATCCCTG
    GAAAGACTAC TCAAAGTGTT CCAAGGAGGA AAAGAAGTAC AGGATGCTAA CAGATTCACC
    AGCCACCTAC AGATAGAGAA CACTTACTCA GAGAACTTCG TAAAAGTATT TCAAGACCTA
    GGTGCCAAGG ATCTGGAGCC AGTTGAAGTT GCTGTTCTCT TGAAGCATCC TTTTATTCAA
    GACCTGATTT CAAATTATTC AGACTATAAA ATCCCTGATA TTAAAATAAT TCTCCAAAGG
    AGTGAGCATG TACAAGGAAG TGATGGAGAA AGATCTCCTT CAAGACTTAC AGAGGAAAAG
    AAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05532
    Clone ID Related Accession (Same CDS sequence) XM_546353.3 , XM_546353.4
    Accession Version XM_546353.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5466bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product sperm flagellar protein 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_546353.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGAGA TTCTGTGCCA GTGGCTCAAC CAGGAGGTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAAT TCTGCACAAG
    TTTGAACTTC AGGGTGATTT TTCGGAATTT TCAGAAAGCA GAGTTTCAAA TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCGACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCACA ACATCATAAC GGAAAAGCCT GGGGCGGCAA CCAAACTCTT GTATCAGTTG
    TACATTGTTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAATAGAAAT GCAAACTATG
    CAACCCCTGG CAAACGTCAG ATACCAAAGT ATGAAAAGTG AGGCTTTTCG AGATAGACTT
    AGAAACCTGA TACCACGTCA AACTGATTTC AATCTGATGC AGGTTACACA CAGGTTTCAA
    GAAAAATATA AACACATGAA AGAAGATCTT GTCCACATGC ATTTTGAGAA ACTTGAAAAA
    TTTCAAAAAC TCAAGGAAGA ACAAAGATGT TTTAATATTG AAAAGCAACG CCTGAGCAGG
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TCATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAG AGCACTGGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAGGATG TGGCCAATGA GATTAAGAAG TTTGAAGCTT TAATAAAAAA GGATCTCCAG
    GCCAAAGAAA GTGCCTCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC TACTGATTTG
    TTAAACACTT ATTCGGATGA TGACTACATT AAAAAAATCC AAAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAGAC TATTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCGTATCGG GAGGAGCAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATCTCTGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTGTGGC AAAACAGAAT TTTCCGAGAA AAACAGTATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTCAG
    GAACAAGCCA TTAGAGAGAA GGAAATTCAT GAACAGATCA CTGTGGAAAG AGCTCAAGCT
    CGTTACAAAA AGCATTATAC AATATGTGCA GAAATTTTGG ATCAAATACT TGATCTTTCC
    ACTAAAGTGT CAGACTACCG GATGTTGACA AATAATTTGA TTCCATATAA GTTGATGCAT
    GATTGGAAGG AACTGTTTTT TAATGGAAAG CCCATATACA AGCAAGCCTC TATTAAGCAA
    CTACCAGCCA CGCCCTCTGC AGAACAAGTT ATAGAACTGG AGAAGAGGGA CTTGCTGGAT
    AGCAATGATT ATGAAGAATA TAAGAATATG GTTGGAGAGT GGGCTTTACC AGAAGAAATG
    GTTGATAATT TACCACCCTC CAACAATACC ATATTGGGTC ATGTGATTCA CAGATTAATT
    GAAAAATCTC ATCCTCCTCA AGTCAAATCA ACAGTACTAG AATTACCTTC ATTTGCTATT
    AAAGGGTGCT TAGTTGGAAA AACATTTAGT GGAAAAACTA CTGCTCTAAA GGCTCTACAA
    AAAGACTTTC CTATTCAGGT ACTTTCTATT GACACTCTTG TCCAAGAAGC AATCAAAGCA
    TTCCATGACA ATGAAAAAAT CAGTGAGGCT CTACCAATTG AGAAAGAAGC TGAAGAAAAG
    TCTCTACCAG TTCTGCAAGA GAAGAACAAA GAAAGCCAGG ATCTACAGAA TGCATTTTCA
    CCTCATCCAG TTTCAGAAGA AGCGTTACCA GAAACAGAAG ATAAAACTAT ACTTAGTACT
    GATACAAATA AAACTCCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTCTGAACTG
    ACTGTGCGGG CTCAGCTTGG TGCAAAATCA GAAAAGATGC TGAAGAAAGG AAAGAGCATT
    TCTGACATGT TGCTAGTTAA TATCTTGGTA AATGCTATTA ATGAGGTACC TGTGGATCAA
    GGCTGGGTCT TGGATGGTTT TCCAATGACG TTAAACCAAG CAAAGCTTCT GGAGGAAGCT
    CTCACGGGCT ACAACAGAGA GGCCCTAGAA CTGGAAGAAA AAAAAACACA AATACCCACA
    TTGGCTGTTG ATGCTAGCAC CTCCAAAGAA GTGCCTCTTC CCCCTTCTGC ATTTGACTTT
    GTCATGTTAT TAGATATTTC AGATAATTCC TTGCTGGACC GCATGAATTA CACAATGGCT
    GAAGCATTTT CAAGTGAAAC TTCTCATGAA GACATCACTC AACGTGTAAC TGCTGAAAAG
    CAAGATATGA ATGAGGACCA GAATTTAAGA GACCAGATTC AGCACAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTGTT GGAGCAATGG TTTACAGAGC CAGAAAATAT TTTGATAAAT
    ATCAATGCTG AAGTAGATAA AGAGTCTTTA TGCAATAAAG TAAAAGAAAT GTTTATGACT
    GAAATAATGA AAAAAGAGAA TAAAGTTAAA AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGAAGTTC CCCTGGGTGA GCCTCCGCCT CCTCCTCCTC CCCCTCCCCC TCCTCCTCCT
    GAGCCAGAAA AAGAGAAGGA AACCCATCCT CAACGAGAGC ATTCAAAAAC TCCTACTGCA
    AAAGGGAAAC CACCAGCAGA ACCCTTGACT GGTAAGCCAG ACTCTCTTCA GGAAGGAAAA
    GGAAAGAAAG GGGACACTTC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCACCAGGA
    GGGAAAGGAC CACTAAAGAA ATCACCTGCT GATTCTACAG ACATGTCACC TGTTCCCATA
    GTGCCACCAC CACCCAAGCC AGGATCAGAA GAATGGGTCT ACGTGAATGA ACCAATTCCT
    GAGGAAATGC CTTCATTTTT AGTGCCTTAC TGGGAACTAA TCGAAAATTC CTACATAAAC
    TCCATCAAAA CAGTACTCAG GCATCTGAGA GAAGAACAGC ATAGTGTGGT TGCTTACTTA
    TACGAGGTTA GAACAAATTT CCAGCAGTTT CTATGGCGTC CAGATCACAA GCAAGATTTT
    GTATCTCAAT GGCAGGCTGA TTTCAATGCT CTTCCTGATG ACCTGTGGGA TGATGAAGAA
    ACAAAGTCTG AACTACACCA AAGAGTAAAT GATTTAAGAG ACCGCCTGTG GGACATCTGT
    GATGCCCGGA AGGAAGAAGC AGAGCAGGAG CGTCAGGATA TCATTAACGA GAGCTGGTTG
    CAAGACTCAA TCGGAATAAC GATGAACCAT TTCTTCTCAC TAATGCAGAC AGAACTGAAC
    CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACA GGGGAATGGA ATGCAAAATT
    CCAATCGATG ACAGTAAGAG ATTTACTCGA ATCCCGTTGG TCCAGCTGGA TAGTAGTAGA
    GACATTTTGG AAAGCCAACT CAGAATTCCT CTAATTCCAC GAATATCCAT TTCCCCGGAT
    TCCGCTATGA CCAAACCAAA AACAAAGGCC ATACTAAAGG GCAAGATCGA TTACTCGCTG
    GAAAATGTGG AGTTAAACTT TGAGGCAGAT GAGAAGTTGG TGATGGACAC CTGGCAGCAG
    GCTTCTTTAG CAATATCTCA CATGGTGGCT GCTGAAATTC ATCAGAGGCT CATGGAAGAA
    GAAAAGGAAA ACCAACCAGC AGACACAAAA GAAAAATCTC CTCAGATGGG GACAAATAAA
    AAAGCCAAAG AGGAACCACC CAAGAAAGAA AAGGCAGACA AAAAAGCAAA AGGCAGATCC
    CCGCCGACAG CAGAAGCCAC GCCTGTGACA GTCACAGCAG AGGAGATTAC CCAAAAGGAG
    AAGAGAAACG AACTGATGCT CAAAATCAAG GAAGAGCACC TCGCTGCCCT GCAGTTTGAA
    GAGATAGCCA CACAGTTCCG GCTTGAACTG ATAAAGACAA AAGCATTGGC TTTCCTTGAA
    GATTTACTAA CAAAGGTGGT CGATGTATAT AAACTCTTGG AAAAGTGGCT TGGTGAGAGG
    TACTTGAATG AAATGGCCAG TGTGGAAAAA TTAACTGAGG TGGCCCGTTA TCACATTGAA
    ACATCAACAA AAATTCAGAA TGAACTTTAC CTAGACCAAG AAGATTTCTT CATTAATGGA
    GATCTGAAAG TCTTTCCAGA TCCCCCTCCC CCAGTGCGCC CCCCACCTGT GGAAAAGGAA
    GAAAATGGCA CCCTGACCAT CGAACAGCTT GACAGTCTTA AAGATCAGTT CTTAGATATT
    GCACCCAAAG GCATAATAGG ACATAAAGCA TTTACTGACA TTCTGCTGGA TCTGGTAACC
    CTGAACCTTG GAACAAACAA CTTACCTAGT AGTTGGATGC ACCTCACGCA ACCAGAATTG
    CAGGAGTTAA CATCTTTAGT AATAGTAAAT TCGGAGTTTG TGGACTGGCG GAAATTCCTA
    TTAGTAATCT CGATGCCTTG GCCCATCCCC ATGGAGGAAG AGCTCCTCGA AACCCTGCAG
    AGGTTCAAGG CGGTGGATGA AGAGCAGTTG GGCACCATCA CTTTTGAGCA GTATATGCAG
    GCTGGTCTGT GGTTTACAGG AGATGAATAT ATAAAAATTC CAGAAAATCC TCTTGAACCT
    CTGCCGTTCA ATAGGCAGGA GCATCTTATA GAGTTTTTCT TTAGGCTATT TGCTGACCAT
    GAGAAGGATC CACCTCAACT TGACTACACA CAGATGCTGC TCTATTTTGC TTGCCATCCA
    GATGCTATGG AAGGGGTCTA CAGGGCCCTC AGTGTGGCCA TAGGAACTCA TGTCTTCCAA
    CCAGTCGAAA CTATTGCTAT TATTGGAGAA AAGTCCTCCT CCTTGACTGA TGCAAGTCCA
    GTTGAGGAGT ATCCTGAACT CGAGGGAAAC TTTATGCATG AGGAAGGAGA ATTAAAAGAA
    GACAGGGAGG AAAAGGAAGT AGAAATCCCT GAGAATGCAA ACACTGAAAA GGTATCCCTG
    GAAAGACTAC TCAAAGTGTT CCAAGGAGGA AAAGAAGTAC AGGATGCTAA CAGATTCACC
    AGCCACCTAC AGATAGAGAA CACTTACTCA GAGAACTTCG TAAAAGTATT TCAAGACCTA
    GGTGCCAAGG ATCTGGAGCC AGTTGAAGTT GCTGTTCTCT TGAAGCATCC TTTTATTCAA
    GACCTGATTT CAAATTATTC AGACTATAAA ATCCCTGATA TTAAAATAAT TCTCCAAAGG
    AGTGAGCATG TACAAGGAAG TGATGGAGAA AGATCTCCTT CAAGACTTAC AGAGGAAAAG
    AAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_546353.2
    CDS159..5624
    Translation

    Target ORF information:

    RefSeq Version XM_546353.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris sperm flagellar protein 2 (LOC489235), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_546353.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGAGA TTCTGTGCCA GTGGCTCAAC CAGGAGGTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAAT TCTGCACAAG
    TTTGAACTTC AGGGTGATTT TTCGGAATTT TCAGAAAGCA GAGTTTCAAA TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCGACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCACA ACATCATAAC GGAAAAGCCT GGGGCGGCAA CCAAACTCTT GTATCAGTTG
    TACATTGTTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAATAGAAAT GCAAACTATG
    CAACCCCTGG CAAACGTCAG ATACCAAAGT ATGAAAAGTG AGGCTTTTCG AGATAGACTT
    AGAAACCTGA TACCACGTCA AACTGATTTC AATCTGATGC AGGTTACACA CAGGTTTCAA
    GAAAAATATA AACACATGAA AGAAGATCTT GTCCACATGC ATTTTGAGAA ACTTGAAAAA
    TTTCAAAAAC TCAAGGAAGA ACAAAGATGT TTTAATATTG AAAAGCAACG CCTGAGCAGG
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TCATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAG AGCACTGGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAGGATG TGGCCAATGA GATTAAGAAG TTTGAAGCTT TAATAAAAAA GGATCTCCAG
    GCCAAAGAAA GTGCCTCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC TACTGATTTG
    TTAAACACTT ATTCGGATGA TGACTACATT AAAAAAATCC AAAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAGAC TATTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCGTATCGG GAGGAGCAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATCTCTGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTGTGGC AAAACAGAAT TTTCCGAGAA AAACAGTATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTCAG
    GAACAAGCCA TTAGAGAGAA GGAAATTCAT GAACAGATCA CTGTGGAAAG AGCTCAAGCT
    CGTTACAAAA AGCATTATAC AATATGTGCA GAAATTTTGG ATCAAATACT TGATCTTTCC
    ACTAAAGTGT CAGACTACCG GATGTTGACA AATAATTTGA TTCCATATAA GTTGATGCAT
    GATTGGAAGG AACTGTTTTT TAATGGAAAG CCCATATACA AGCAAGCCTC TATTAAGCAA
    CTACCAGCCA CGCCCTCTGC AGAACAAGTT ATAGAACTGG AGAAGAGGGA CTTGCTGGAT
    AGCAATGATT ATGAAGAATA TAAGAATATG GTTGGAGAGT GGGCTTTACC AGAAGAAATG
    GTTGATAATT TACCACCCTC CAACAATACC ATATTGGGTC ATGTGATTCA CAGATTAATT
    GAAAAATCTC ATCCTCCTCA AGTCAAATCA ACAGTACTAG AATTACCTTC ATTTGCTATT
    AAAGGGTGCT TAGTTGGAAA AACATTTAGT GGAAAAACTA CTGCTCTAAA GGCTCTACAA
    AAAGACTTTC CTATTCAGGT ACTTTCTATT GACACTCTTG TCCAAGAAGC AATCAAAGCA
    TTCCATGACA ATGAAAAAAT CAGTGAGGCT CTACCAATTG AGAAAGAAGC TGAAGAAAAG
    TCTCTACCAG TTCTGCAAGA GAAGAACAAA GAAAGCCAGG ATCTACAGAA TGCATTTTCA
    CCTCATCCAG TTTCAGAAGA AGCGTTACCA GAAACAGAAG ATAAAACTAT ACTTAGTACT
    GATACAAATA AAACTCCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTCTGAACTG
    ACTGTGCGGG CTCAGCTTGG TGCAAAATCA GAAAAGATGC TGAAGAAAGG AAAGAGCATT
    TCTGACATGT TGCTAGTTAA TATCTTGGTA AATGCTATTA ATGAGGTACC TGTGGATCAA
    GGCTGGGTCT TGGATGGTTT TCCAATGACG TTAAACCAAG CAAAGCTTCT GGAGGAAGCT
    CTCACGGGCT ACAACAGAGA GGCCCTAGAA CTGGAAGAAA AAAAAACACA AATACCCACA
    TTGGCTGTTG ATGCTAGCAC CTCCAAAGAA GTGCCTCTTC CCCCTTCTGC ATTTGACTTT
    GTCATGTTAT TAGATATTTC AGATAATTCC TTGCTGGACC GCATGAATTA CACAATGGCT
    GAAGCATTTT CAAGTGAAAC TTCTCATGAA GACATCACTC AACGTGTAAC TGCTGAAAAG
    CAAGATATGA ATGAGGACCA GAATTTAAGA GACCAGATTC AGCACAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTGTT GGAGCAATGG TTTACAGAGC CAGAAAATAT TTTGATAAAT
    ATCAATGCTG AAGTAGATAA AGAGTCTTTA TGCAATAAAG TAAAAGAAAT GTTTATGACT
    GAAATAATGA AAAAAGAGAA TAAAGTTAAA AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGAAGTTC CCCTGGGTGA GCCTCCGCCT CCTCCTCCTC CCCCTCCCCC TCCTCCTCCT
    GAGCCAGAAA AAGAGAAGGA AACCCATCCT CAACGAGAGC ATTCAAAAAC TCCTACTGCA
    AAAGGGAAAC CACCAGCAGA ACCCTTGACT GGTAAGCCAG ACTCTCTTCA GGAAGGAAAA
    GGAAAGAAAG GGGACACTTC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCACCAGGA
    GGGAAAGGAC CACTAAAGAA ATCACCTGCT GATTCTACAG ACATGTCACC TGTTCCCATA
    GTGCCACCAC CACCCAAGCC AGGATCAGAA GAATGGGTCT ACGTGAATGA ACCAATTCCT
    GAGGAAATGC CTTCATTTTT AGTGCCTTAC TGGGAACTAA TCGAAAATTC CTACATAAAC
    TCCATCAAAA CAGTACTCAG GCATCTGAGA GAAGAACAGC ATAGTGTGGT TGCTTACTTA
    TACGAGGTTA GAACAAATTT CCAGCAGTTT CTATGGCGTC CAGATCACAA GCAAGATTTT
    GTATCTCAAT GGCAGGCTGA TTTCAATGCT CTTCCTGATG ACCTGTGGGA TGATGAAGAA
    ACAAAGTCTG AACTACACCA AAGAGTAAAT GATTTAAGAG ACCGCCTGTG GGACATCTGT
    GATGCCCGGA AGGAAGAAGC AGAGCAGGAG CGTCAGGATA TCATTAACGA GAGCTGGTTG
    CAAGACTCAA TCGGAATAAC GATGAACCAT TTCTTCTCAC TAATGCAGAC AGAACTGAAC
    CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACA GGGGAATGGA ATGCAAAATT
    CCAATCGATG ACAGTAAGAG ATTTACTCGA ATCCCGTTGG TCCAGCTGGA TAGTAGTAGA
    GACATTTTGG AAAGCCAACT CAGAATTCCT CTAATTCCAC GAATATCCAT TTCCCCGGAT
    TCCGCTATGA CCAAACCAAA AACAAAGGCC ATACTAAAGG GCAAGATCGA TTACTCGCTG
    GAAAATGTGG AGTTAAACTT TGAGGCAGAT GAGAAGTTGG TGATGGACAC CTGGCAGCAG
    GCTTCTTTAG CAATATCTCA CATGGTGGCT GCTGAAATTC ATCAGAGGCT CATGGAAGAA
    GAAAAGGAAA ACCAACCAGC AGACACAAAA GAAAAATCTC CTCAGATGGG GACAAATAAA
    AAAGCCAAAG AGGAACCACC CAAGAAAGAA AAGGCAGACA AAAAAGCAAA AGGCAGATCC
    CCGCCGACAG CAGAAGCCAC GCCTGTGACA GTCACAGCAG AGGAGATTAC CCAAAAGGAG
    AAGAGAAACG AACTGATGCT CAAAATCAAG GAAGAGCACC TCGCTGCCCT GCAGTTTGAA
    GAGATAGCCA CACAGTTCCG GCTTGAACTG ATAAAGACAA AAGCATTGGC TTTCCTTGAA
    GATTTACTAA CAAAGGTGGT CGATGTATAT AAACTCTTGG AAAAGTGGCT TGGTGAGAGG
    TACTTGAATG AAATGGCCAG TGTGGAAAAA TTAACTGAGG TGGCCCGTTA TCACATTGAA
    ACATCAACAA AAATTCAGAA TGAACTTTAC CTAGACCAAG AAGATTTCTT CATTAATGGA
    GATCTGAAAG TCTTTCCAGA TCCCCCTCCC CCAGTGCGCC CCCCACCTGT GGAAAAGGAA
    GAAAATGGCA CCCTGACCAT CGAACAGCTT GACAGTCTTA AAGATCAGTT CTTAGATATT
    GCACCCAAAG GCATAATAGG ACATAAAGCA TTTACTGACA TTCTGCTGGA TCTGGTAACC
    CTGAACCTTG GAACAAACAA CTTACCTAGT AGTTGGATGC ACCTCACGCA ACCAGAATTG
    CAGGAGTTAA CATCTTTAGT AATAGTAAAT TCGGAGTTTG TGGACTGGCG GAAATTCCTA
    TTAGTAATCT CGATGCCTTG GCCCATCCCC ATGGAGGAAG AGCTCCTCGA AACCCTGCAG
    AGGTTCAAGG CGGTGGATGA AGAGCAGTTG GGCACCATCA CTTTTGAGCA GTATATGCAG
    GCTGGTCTGT GGTTTACAGG AGATGAATAT ATAAAAATTC CAGAAAATCC TCTTGAACCT
    CTGCCGTTCA ATAGGCAGGA GCATCTTATA GAGTTTTTCT TTAGGCTATT TGCTGACCAT
    GAGAAGGATC CACCTCAACT TGACTACACA CAGATGCTGC TCTATTTTGC TTGCCATCCA
    GATGCTATGG AAGGGGTCTA CAGGGCCCTC AGTGTGGCCA TAGGAACTCA TGTCTTCCAA
    CCAGTCGAAA CTATTGCTAT TATTGGAGAA AAGTCCTCCT CCTTGACTGA TGCAAGTCCA
    GTTGAGGAGT ATCCTGAACT CGAGGGAAAC TTTATGCATG AGGAAGGAGA ATTAAAAGAA
    GACAGGGAGG AAAAGGAAGT AGAAATCCCT GAGAATGCAA ACACTGAAAA GGTATCCCTG
    GAAAGACTAC TCAAAGTGTT CCAAGGAGGA AAAGAAGTAC AGGATGCTAA CAGATTCACC
    AGCCACCTAC AGATAGAGAA CACTTACTCA GAGAACTTCG TAAAAGTATT TCAAGACCTA
    GGTGCCAAGG ATCTGGAGCC AGTTGAAGTT GCTGTTCTCT TGAAGCATCC TTTTATTCAA
    GACCTGATTT CAAATTATTC AGACTATAAA ATCCCTGATA TTAAAATAAT TCTCCAAAGG
    AGTGAGCATG TACAAGGAAG TGATGGAGAA AGATCTCCTT CAAGACTTAC AGAGGAAAAG
    AAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36160
    Clone ID Related Accession (Same CDS sequence) XM_014113141.1
    Accession Version XM_014113141.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product sperm flagellar protein 2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTCGGAGA TTCTGTGCCA GTGGCTCAAC CAGGAGGTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAAT TCTGCACAAG
    TTTGAACTTC AGGGTGATTT TTCGGAATTT TCAGAAAGCA GAGTTTCAAA TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCGACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCACA ACATCATAAC GGAAAAGCCT GGGGCGGCAA CCAAACTCTT GTATCAGTTG
    TACATTGTTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAATAGAAAT GCAAACTATG
    CAACCCCTGG CAAACGTCAG ATACCAAAGT ATGAAAAGTG AGGCTTTTCG AGATCAACGC
    CTGAGCAGGA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCTG CATCAAATCG TACTTTGAGA GCACTGGAGG CCCAAAAAAT GATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAG ATTAAGAAGT TTGAAGCTTT AATAAAAAAG
    GATCTCCAGG CCAAAGAAAG TGCCTCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACT
    ACTGATTTGT TAAACACTTA TTCGGATGAT GACTACATTA AAAAAATCCA AAAGCGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT ATTAATGGAC
    CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCGTATCGGG AGGAGCAGCT GATTAACCGG
    CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATCTCTGTGC AGCTCATGCA TGTTCGGCAT
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCCGAGAAA AACAGTATGA GGAAAGACGA
    CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT
    GATTTTCAGG AACAAGCCAT TAGAGAGAAG GAAATTCATG AACAGATCAC TGTGGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATACA ATATGTGCAG AAATTTTGGA TCAAATACTT
    GATCTTTCCA CTAAAGTGTC AGACTACCGG ATGTTGACAA ATAATTTGAT TCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT AATGGAAAGC CCATATACAA GCAAGCCTCT
    ATTAAGCAAC TACCAGCCAC GCCCTCTGCA GAACAAGTTA TAGAACTGGA GAAGAGGGAC
    TTGCTGGATA GCAATGATTA TGAAGAATAT AAGAATATGG TTGGAGAGTG GGCTTTACCA
    GAAGAAATGG TTGATAATTT ACCACCCTCC AACAATACCA TATTGGGTCA TGTGATTCAC
    AGATTAATTG AAAAATCTCA TCCTCCTCAA GTCAAATCAA CAGTACTAGA ATTACCTTCA
    TTTGCTATTA AAGGGTGCTT AGTTGGAAAA ACATTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTACAAA AAGACTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT CCAAGAAGCA
    ATCAAAGCAT TCCATGACAA TGAAAAAATC AGTGAGGCTC TACCAATTGA GAAAGAAGCT
    GAAGAAAAGT CTCTACCAGT TCTGCAAGAG AAGAACAAAG AAAGCCAGGA TCTACAGAAT
    GCATTTTCAC CTCATCCAGT TTCAGAAGAA GCGTTACCAG AAACAGAAGA TAAAACTATA
    CTTAGTACTG ATACAAATAA AACTCCAAAA GCTGAAGAAG TCAAATCAAG TGATAGTTTC
    TCTGAACTGA CTGTGCGGGC TCAGCTTGGT GCAAAATCAG AAAAGATGCT GAAGAAAGGA
    AAGAGCATTT CTGACATGTT GCTAGTTAAT ATCTTGGTAA ATGCTATTAA TGAGGTACCT
    GTGGATCAAG GCTGGGTCTT GGATGGTTTT CCAATGACGT TAAACCAAGC AAAGCTTCTG
    GAGGAAGCTC TCACGGGCTA CAACAGAGAG GCCCTAGAAC TGGAAGAAAA AAAAACACAA
    ATACCCACAT TGGCTGTTGA TGCTAGCACC TCCAAAGAAG TGCCTCTTCC CCCTTCTGCA
    TTTGACTTTG TCATGTTATT AGATATTTCA GATAATTCCT TGCTGGACCG CATGAATTAC
    ACAATGGCTG AAGCATTTTC AAGTGAAACT TCTCATGAAG ACATCACTCA ACGTGTAACT
    GCTGAAAAGC AAGATATGAA TGAGGACCAG AATTTAAGAG ACCAGATTCA GCACAGAATT
    ATAGGCTTCT TGGACAACTG GCCTTTGTTG GAGCAATGGT TTACAGAGCC AGAAAATATT
    TTGATAAATA TCAATGCTGA AGTAGATAAA GAGTCTTTAT GCAATAAAGT AAAAGAAATG
    TTTATGACTG AAATAATGAA AAAAGAGAAT AAAGTTAAAA AGAAATTAGA AGAAAAGGAA
    GCTGAGAAAA AAGAAGTTCC CCTGGGTGAG CCTCCGCCTC CTCCTCCTCC CCCTCCCCCT
    CCTCCTCCTG AGCCAGAAAA AGAGAAGGAA ACCCATCCTC AACGAGAGCA TTCAAAAACT
    CCTACTGCAA AAGGGAAACC ACCAGCAGGT TCTCCTAAAG GAAAATCACC AGGAGGGAAA
    GGACCACTAA AGAAATCACC TGCTGATTCT ACAGACATGT CACCTGTTCC CATAGTGCCA
    CCACCACCCA AGCCAGGATC AGAAGAATGG GTCTACGTGA ATGAACCAAT TCCTGAGGAA
    ATGCCTTCAT TTTTAGTGCC TTACTGGGAA CTAATCGAAA ATTCCTACAT AAACTCCATC
    AAAACAGTAC TCAGGCATCT GAGAGAAGAA CAGCATAGTG TGGTTGCTTA CTTATACGAG
    GTTAGAACAA ATTTCCAGCA GTTTCTATGG CGTCCAGATC ACAAGCAAGA TTTTGTATCT
    CAATGGCAGG CTGATTTCAA TGCTCTTCCT GATGACCTGT GGGATGATGA AGAAACAAAG
    TCTGAACTAC ACCAAAGAGT AAATGATTTA AGAGACCGCC TGTGGGACAT CTGTGATGCC
    CGGAAGGAAG AAGCAGAGCA GGAGCGTCAG GATATCATTA ACGAGAGCTG GTTGCAAGAC
    TCAATCGGAA TAACGATGAA CCATTTCTTC TCACTAATGC AGACAGAACT GAACCGTTTC
    CAAGATACAA AGAGACTCCT TCAAGATTAT TACAGGGGAA TGGAATGCAA AATTCCAATC
    GATGACAGTA AGAGATTTAC TCGAATCCCG TTGGTCCAGC TGGATAGTAG TAGAGACATT
    TTGGAAAGCC AACTCAGAAT TCCTCTAATT CCACGAATAT CCATTTCCCC GGATTCCGCT
    ATGACCAAAC CAAAAACAAA GGCCATACTA AAGGGCAAGA TCGATTACTC GCTGGAAAAT
    GTGGAGTTAA ACTTTGAGGC AGATGAGAAG TTGGTGATGG ACACCTGGCA GCAGGCTTCT
    TTAGCAATAT CTCACATGGT GGCTGCTGAA ATTCATCAGA GGCTCATGGA AGAAGAAAAG
    GAAAACCAAC CAGCAGACAC AAAAGAAAAA TCTCCTCAGA TGGGGACAAA TAAAAAAGCC
    AAAGAGGAAC CACCCAAGAA AGAAAAGGCA GACAAAAAAG CAAAAGGCAG ATCCCCGCCG
    ACAGCAGAAG CCACGCCTGT GACAGTCACA GCAGAGGAGA TTACCCAAAA GGAGAAGAGA
    AACGAACTGA TGCTCAAAAT CAAGGAAGAG CACCTCGCTG CCCTGCAGTT TGAAGAGATA
    GCCACACAGT TCCGGCTTGA ACTGATAAAG ACAAAAGCAT TGGCTTTCCT TGAAGATTTA
    CTAACAAAGG TGGTCGATGT ATATAAACTC TTGGAAAAGT GGCTTGGTGA GAGGTACTTG
    AATGAAATGG CCAGTGTGGA AAAATTAACT GAGGTGGCCC GTTATCACAT TGAAACATCA
    ACAAAAATTC AGAATGAACT TTACCTAGAC CAAGAAGATT TCTTCATTAA TGGAGATCTG
    AAAGTCTTTC CAGATCCCCC TCCCCCAGTG CGCCCCCCAC CTGTGGAAAA GGAAGAAAAT
    GGCACCCTGA CCATCGAACA GCTTGACAGT CTTAAAGATC AGTTCTTAGA TATTGCACCC
    AAAGGCAGGG ATCCTCCAGG TGACATGAAC CGAGTCACTG AAAAGCAGAA AAGGCTGCAG
    AAGCTTGACC ATTACCTTCC AAAAAGGAAC GATCAATTTT TGAAAATCTG GCTGAAAAAA
    TACCCTTGGC TTATATATGA TGAGATGTTA AATCTTATGT TCTGTGGCCT GTGTCGTAAA
    CATGGAGTGA AGTCAAAGGG AAATCAAGTG AGTTTTTTTT ATGGCACTGA CAACTTTAGG
    ACAGAATTTC TCAATGCACA TCATCTCAGT GAGGCTCATG CCAAGGCTTC ACTAATGGAA
    GCAACAAATG GTTCTCCAGT GAATGTCGCC ACCACAGAGC TCATGGTGAC AACCATGAGC
    AAAATCACCC TCGGGAGAGT AGAGAACTTA TTCAGATCAT GCCATGCTAT CGCCAAGACG
    GGACGTCCTC TCAAAGACTT TATCTGGATG TGCAAGTTGG ATGATATGAA GGGGGTCGAT
    ATCGGCCCCG TATTCAGAAC TAACAAATCA GCAAGAACCT TTACATATTT TATTGCTGAA
    GTGGAACGGA AAAATCTTAG GGAGAAACTA GAACAAAGTA AATTTTTCTC TGTCCTCAGT
    GACGGAATCA CAGACAGTTC AACCAAGGCG GCTGAACTTG TCTACGTGCA GTTTGCACAC
    GCGGGAAAAG TGCATTGCCA AATTGCTGGG GTACAGACAG TAGAAAGGGA GGATCCTTTG
    GCAATAAGAG ATGCCATTGA AAGAACTCTA GAGAGAAATC TTCAACTTAA GCTATCAGAC
    CAAGACTGGG CCAAGAAACT GGTTGGTTTT GGAAGTGATG GTGCCCTTGG CTCAGGGAGA
    GAAAACAGCA GGGTGGCCCT GCTTTTAAGA GAAATCCAGC CATGTGCTCT GACTGTATAC
    TGCTTTGCAC ACCATCTTGA ACTGTCTTAC AAGGCGGTGT TCCAAAGCGT TCCCCTGTAC
    AACGACGTCA GAAATCTTCT TCACAGCATC TACCACTTCT ACCACAGTTC CCCTCTGCAC
    AGGAGTTCTC TGGTAACTGC TTTCAAAGGC CTTCACCTTC GACCTGTGAT GCCTTCTCAA
    GTAGGGGGCA GGAGGTGGCT TCAGGAACTA CAGGCAGCTC TCCAGAACTT TCTCAAGGGA
    TATCCTGCAA TAGTCCAGCA ACTGCATTCG GGAGATGAAG GCAGGAGTGA CACCAGTCAT
    CAGAAAGCCA AGGAACTCCT GGGGTTCCTT CTCCAAGCAA ACATTGTTAA ATTTATCCAC
    TTCTTAGTGG ATGTCATTAA TGTCCTTAGC ATTCTTTCCC GTGTCAATCA AAACAGAAAT
    TCTTCCATTG CTGATGTATT TGCCACATTA GAATCAACAC TGGAAATGCT CAGAATATAT
    CAAACAAGAC CAGGACCAAA GGAACGTTGG GTGGATTCGG TCACGCACTT TCATGGCAAC
    TGCCTGGGAG GAAAGGGAGA CATTTCTGAT GTGAGAAGAG TAGTCTTAAC ACATCTTATC
    AAGAGGCTGC AAGGTTGCTT CAGAGATGCC AGCCAAGATG TGCTGAAGGC AACCATGATT
    GGAAGCTTTA AACTGTGGCC TGCGAAGATA AATCAAGAAT TTGGAGAAAA AGAGGTATCC
    ACCCTGATTG CCCATTATGA ACCAGTACTG GAAGCTGCCA ACGTGGAAAT CGACGAAGTG
    GACGCTGAGT GGAGCATGCT GAAATTAGAG ATTTATGCTA GATTTCAGAA CATCCGCAAA
    TTGACCTGGG ATTTTGTGAA TTCTGTTTAC TTACACAAGT ATCCAAATAT CTTGACGTTA
    GTGGACCTCG TGCTGACCCT CCCTGCAAGT TCCGCGGAGG CAGAACGTGG CTTTAGTCAG
    ATGAAACTCA CCAAGTCACA AATGCGTGCC AAAATCAAGG CTGAAAGTAT GACGGATCTC
    CTTGTAATTC AGTTGAATTC TCCGGACATT AATAACTTTG ACCCTCGGAA AGCTATCCAT
    TTATGGAACA CAAGAACACC ATCTTCAGCT GATGACACAA GACCTAATTC TGCTTATTCA
    TCAAACTCCG AAAGCCATGA TGAAAGCGAT TCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968616.1
    CDS159..6434
    Translation

    Target ORF information:

    RefSeq Version XM_014113141.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113141.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTCGGAGA TTCTGTGCCA GTGGCTCAAC CAGGAGGTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAAT TCTGCACAAG
    TTTGAACTTC AGGGTGATTT TTCGGAATTT TCAGAAAGCA GAGTTTCAAA TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCGACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCACA ACATCATAAC GGAAAAGCCT GGGGCGGCAA CCAAACTCTT GTATCAGTTG
    TACATTGTTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAATAGAAAT GCAAACTATG
    CAACCCCTGG CAAACGTCAG ATACCAAAGT ATGAAAAGTG AGGCTTTTCG AGATCAACGC
    CTGAGCAGGA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCTG CATCAAATCG TACTTTGAGA GCACTGGAGG CCCAAAAAAT GATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAG ATTAAGAAGT TTGAAGCTTT AATAAAAAAG
    GATCTCCAGG CCAAAGAAAG TGCCTCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACT
    ACTGATTTGT TAAACACTTA TTCGGATGAT GACTACATTA AAAAAATCCA AAAGCGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT ATTAATGGAC
    CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCGTATCGGG AGGAGCAGCT GATTAACCGG
    CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATCTCTGTGC AGCTCATGCA TGTTCGGCAT
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCCGAGAAA AACAGTATGA GGAAAGACGA
    CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT
    GATTTTCAGG AACAAGCCAT TAGAGAGAAG GAAATTCATG AACAGATCAC TGTGGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATACA ATATGTGCAG AAATTTTGGA TCAAATACTT
    GATCTTTCCA CTAAAGTGTC AGACTACCGG ATGTTGACAA ATAATTTGAT TCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT AATGGAAAGC CCATATACAA GCAAGCCTCT
    ATTAAGCAAC TACCAGCCAC GCCCTCTGCA GAACAAGTTA TAGAACTGGA GAAGAGGGAC
    TTGCTGGATA GCAATGATTA TGAAGAATAT AAGAATATGG TTGGAGAGTG GGCTTTACCA
    GAAGAAATGG TTGATAATTT ACCACCCTCC AACAATACCA TATTGGGTCA TGTGATTCAC
    AGATTAATTG AAAAATCTCA TCCTCCTCAA GTCAAATCAA CAGTACTAGA ATTACCTTCA
    TTTGCTATTA AAGGGTGCTT AGTTGGAAAA ACATTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTACAAA AAGACTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT CCAAGAAGCA
    ATCAAAGCAT TCCATGACAA TGAAAAAATC AGTGAGGCTC TACCAATTGA GAAAGAAGCT
    GAAGAAAAGT CTCTACCAGT TCTGCAAGAG AAGAACAAAG AAAGCCAGGA TCTACAGAAT
    GCATTTTCAC CTCATCCAGT TTCAGAAGAA GCGTTACCAG AAACAGAAGA TAAAACTATA
    CTTAGTACTG ATACAAATAA AACTCCAAAA GCTGAAGAAG TCAAATCAAG TGATAGTTTC
    TCTGAACTGA CTGTGCGGGC TCAGCTTGGT GCAAAATCAG AAAAGATGCT GAAGAAAGGA
    AAGAGCATTT CTGACATGTT GCTAGTTAAT ATCTTGGTAA ATGCTATTAA TGAGGTACCT
    GTGGATCAAG GCTGGGTCTT GGATGGTTTT CCAATGACGT TAAACCAAGC AAAGCTTCTG
    GAGGAAGCTC TCACGGGCTA CAACAGAGAG GCCCTAGAAC TGGAAGAAAA AAAAACACAA
    ATACCCACAT TGGCTGTTGA TGCTAGCACC TCCAAAGAAG TGCCTCTTCC CCCTTCTGCA
    TTTGACTTTG TCATGTTATT AGATATTTCA GATAATTCCT TGCTGGACCG CATGAATTAC
    ACAATGGCTG AAGCATTTTC AAGTGAAACT TCTCATGAAG ACATCACTCA ACGTGTAACT
    GCTGAAAAGC AAGATATGAA TGAGGACCAG AATTTAAGAG ACCAGATTCA GCACAGAATT
    ATAGGCTTCT TGGACAACTG GCCTTTGTTG GAGCAATGGT TTACAGAGCC AGAAAATATT
    TTGATAAATA TCAATGCTGA AGTAGATAAA GAGTCTTTAT GCAATAAAGT AAAAGAAATG
    TTTATGACTG AAATAATGAA AAAAGAGAAT AAAGTTAAAA AGAAATTAGA AGAAAAGGAA
    GCTGAGAAAA AAGAAGTTCC CCTGGGTGAG CCTCCGCCTC CTCCTCCTCC CCCTCCCCCT
    CCTCCTCCTG AGCCAGAAAA AGAGAAGGAA ACCCATCCTC AACGAGAGCA TTCAAAAACT
    CCTACTGCAA AAGGGAAACC ACCAGCAGGT TCTCCTAAAG GAAAATCACC AGGAGGGAAA
    GGACCACTAA AGAAATCACC TGCTGATTCT ACAGACATGT CACCTGTTCC CATAGTGCCA
    CCACCACCCA AGCCAGGATC AGAAGAATGG GTCTACGTGA ATGAACCAAT TCCTGAGGAA
    ATGCCTTCAT TTTTAGTGCC TTACTGGGAA CTAATCGAAA ATTCCTACAT AAACTCCATC
    AAAACAGTAC TCAGGCATCT GAGAGAAGAA CAGCATAGTG TGGTTGCTTA CTTATACGAG
    GTTAGAACAA ATTTCCAGCA GTTTCTATGG CGTCCAGATC ACAAGCAAGA TTTTGTATCT
    CAATGGCAGG CTGATTTCAA TGCTCTTCCT GATGACCTGT GGGATGATGA AGAAACAAAG
    TCTGAACTAC ACCAAAGAGT AAATGATTTA AGAGACCGCC TGTGGGACAT CTGTGATGCC
    CGGAAGGAAG AAGCAGAGCA GGAGCGTCAG GATATCATTA ACGAGAGCTG GTTGCAAGAC
    TCAATCGGAA TAACGATGAA CCATTTCTTC TCACTAATGC AGACAGAACT GAACCGTTTC
    CAAGATACAA AGAGACTCCT TCAAGATTAT TACAGGGGAA TGGAATGCAA AATTCCAATC
    GATGACAGTA AGAGATTTAC TCGAATCCCG TTGGTCCAGC TGGATAGTAG TAGAGACATT
    TTGGAAAGCC AACTCAGAAT TCCTCTAATT CCACGAATAT CCATTTCCCC GGATTCCGCT
    ATGACCAAAC CAAAAACAAA GGCCATACTA AAGGGCAAGA TCGATTACTC GCTGGAAAAT
    GTGGAGTTAA ACTTTGAGGC AGATGAGAAG TTGGTGATGG ACACCTGGCA GCAGGCTTCT
    TTAGCAATAT CTCACATGGT GGCTGCTGAA ATTCATCAGA GGCTCATGGA AGAAGAAAAG
    GAAAACCAAC CAGCAGACAC AAAAGAAAAA TCTCCTCAGA TGGGGACAAA TAAAAAAGCC
    AAAGAGGAAC CACCCAAGAA AGAAAAGGCA GACAAAAAAG CAAAAGGCAG ATCCCCGCCG
    ACAGCAGAAG CCACGCCTGT GACAGTCACA GCAGAGGAGA TTACCCAAAA GGAGAAGAGA
    AACGAACTGA TGCTCAAAAT CAAGGAAGAG CACCTCGCTG CCCTGCAGTT TGAAGAGATA
    GCCACACAGT TCCGGCTTGA ACTGATAAAG ACAAAAGCAT TGGCTTTCCT TGAAGATTTA
    CTAACAAAGG TGGTCGATGT ATATAAACTC TTGGAAAAGT GGCTTGGTGA GAGGTACTTG
    AATGAAATGG CCAGTGTGGA AAAATTAACT GAGGTGGCCC GTTATCACAT TGAAACATCA
    ACAAAAATTC AGAATGAACT TTACCTAGAC CAAGAAGATT TCTTCATTAA TGGAGATCTG
    AAAGTCTTTC CAGATCCCCC TCCCCCAGTG CGCCCCCCAC CTGTGGAAAA GGAAGAAAAT
    GGCACCCTGA CCATCGAACA GCTTGACAGT CTTAAAGATC AGTTCTTAGA TATTGCACCC
    AAAGGCAGGG ATCCTCCAGG TGACATGAAC CGAGTCACTG AAAAGCAGAA AAGGCTGCAG
    AAGCTTGACC ATTACCTTCC AAAAAGGAAC GATCAATTTT TGAAAATCTG GCTGAAAAAA
    TACCCTTGGC TTATATATGA TGAGATGTTA AATCTTATGT TCTGTGGCCT GTGTCGTAAA
    CATGGAGTGA AGTCAAAGGG AAATCAAGTG AGTTTTTTTT ATGGCACTGA CAACTTTAGG
    ACAGAATTTC TCAATGCACA TCATCTCAGT GAGGCTCATG CCAAGGCTTC ACTAATGGAA
    GCAACAAATG GTTCTCCAGT GAATGTCGCC ACCACAGAGC TCATGGTGAC AACCATGAGC
    AAAATCACCC TCGGGAGAGT AGAGAACTTA TTCAGATCAT GCCATGCTAT CGCCAAGACG
    GGACGTCCTC TCAAAGACTT TATCTGGATG TGCAAGTTGG ATGATATGAA GGGGGTCGAT
    ATCGGCCCCG TATTCAGAAC TAACAAATCA GCAAGAACCT TTACATATTT TATTGCTGAA
    GTGGAACGGA AAAATCTTAG GGAGAAACTA GAACAAAGTA AATTTTTCTC TGTCCTCAGT
    GACGGAATCA CAGACAGTTC AACCAAGGCG GCTGAACTTG TCTACGTGCA GTTTGCACAC
    GCGGGAAAAG TGCATTGCCA AATTGCTGGG GTACAGACAG TAGAAAGGGA GGATCCTTTG
    GCAATAAGAG ATGCCATTGA AAGAACTCTA GAGAGAAATC TTCAACTTAA GCTATCAGAC
    CAAGACTGGG CCAAGAAACT GGTTGGTTTT GGAAGTGATG GTGCCCTTGG CTCAGGGAGA
    GAAAACAGCA GGGTGGCCCT GCTTTTAAGA GAAATCCAGC CATGTGCTCT GACTGTATAC
    TGCTTTGCAC ACCATCTTGA ACTGTCTTAC AAGGCGGTGT TCCAAAGCGT TCCCCTGTAC
    AACGACGTCA GAAATCTTCT TCACAGCATC TACCACTTCT ACCACAGTTC CCCTCTGCAC
    AGGAGTTCTC TGGTAACTGC TTTCAAAGGC CTTCACCTTC GACCTGTGAT GCCTTCTCAA
    GTAGGGGGCA GGAGGTGGCT TCAGGAACTA CAGGCAGCTC TCCAGAACTT TCTCAAGGGA
    TATCCTGCAA TAGTCCAGCA ACTGCATTCG GGAGATGAAG GCAGGAGTGA CACCAGTCAT
    CAGAAAGCCA AGGAACTCCT GGGGTTCCTT CTCCAAGCAA ACATTGTTAA ATTTATCCAC
    TTCTTAGTGG ATGTCATTAA TGTCCTTAGC ATTCTTTCCC GTGTCAATCA AAACAGAAAT
    TCTTCCATTG CTGATGTATT TGCCACATTA GAATCAACAC TGGAAATGCT CAGAATATAT
    CAAACAAGAC CAGGACCAAA GGAACGTTGG GTGGATTCGG TCACGCACTT TCATGGCAAC
    TGCCTGGGAG GAAAGGGAGA CATTTCTGAT GTGAGAAGAG TAGTCTTAAC ACATCTTATC
    AAGAGGCTGC AAGGTTGCTT CAGAGATGCC AGCCAAGATG TGCTGAAGGC AACCATGATT
    GGAAGCTTTA AACTGTGGCC TGCGAAGATA AATCAAGAAT TTGGAGAAAA AGAGGTATCC
    ACCCTGATTG CCCATTATGA ACCAGTACTG GAAGCTGCCA ACGTGGAAAT CGACGAAGTG
    GACGCTGAGT GGAGCATGCT GAAATTAGAG ATTTATGCTA GATTTCAGAA CATCCGCAAA
    TTGACCTGGG ATTTTGTGAA TTCTGTTTAC TTACACAAGT ATCCAAATAT CTTGACGTTA
    GTGGACCTCG TGCTGACCCT CCCTGCAAGT TCCGCGGAGG CAGAACGTGG CTTTAGTCAG
    ATGAAACTCA CCAAGTCACA AATGCGTGCC AAAATCAAGG CTGAAAGTAT GACGGATCTC
    CTTGTAATTC AGTTGAATTC TCCGGACATT AATAACTTTG ACCCTCGGAA AGCTATCCAT
    TTATGGAACA CAAGAACACC ATCTTCAGCT GATGACACAA GACCTAATTC TGCTTATTCA
    TCAAACTCCG AAAGCCATGA TGAAAGCGAT TCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.