NFASC cDNA ORF clone, Canis lupus familiaris(dog)

The following NFASC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NFASC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe285029 XM_022415411.1
Latest version!
Canis lupus familiaris neurofascin (NFASC), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe285030 XM_022415412.1
Latest version!
Canis lupus familiaris neurofascin (NFASC), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe285031 XM_022415413.1
Latest version!
Canis lupus familiaris neurofascin (NFASC), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe45309 XM_005640774.3
Latest version!
Canis lupus familiaris neurofascin (NFASC), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe45310 XM_005640775.3
Latest version!
Canis lupus familiaris neurofascin (NFASC), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe45311 XM_005640776.3
Latest version!
Canis lupus familiaris neurofascin (NFASC), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe45312 XM_014111177.2
Latest version!
Canis lupus familiaris neurofascin (NFASC), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe45313 XM_005640777.3
Latest version!
Canis lupus familiaris neurofascin (NFASC), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe45314 XM_005640778.3
Latest version!
Canis lupus familiaris neurofascin (NFASC), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe45315 XM_005640779.3
Latest version!
Canis lupus familiaris neurofascin (NFASC), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe45316 XM_851151.5
Latest version!
Canis lupus familiaris neurofascin (NFASC), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe45317 XM_005640780.3
Latest version!
Canis lupus familiaris neurofascin (NFASC), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe45318 XM_005640781.3
Latest version!
Canis lupus familiaris neurofascin (NFASC), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe45319 XM_545682.6
Latest version!
Canis lupus familiaris neurofascin (NFASC), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe45320 XM_850983.5
Latest version!
Canis lupus familiaris neurofascin (NFASC), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe45321 XM_003434326.4
Latest version!
Canis lupus familiaris neurofascin (NFASC), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe45322 XM_005640782.3
Latest version!
Canis lupus familiaris neurofascin (NFASC), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe31640 XM_005640783.3
Latest version!
Canis lupus familiaris neurofascin (NFASC), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe31640 XM_005640773.3
Latest version!
Canis lupus familiaris neurofascin (NFASC), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe31640 XM_022415410.1
Latest version!
Canis lupus familiaris neurofascin (NFASC), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OCe31640 XM_005640773.2 Canis lupus familiaris neurofascin (NFASC), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe45313 XM_005640777.2 Canis lupus familiaris neurofascin (NFASC), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe45318 XM_005640781.2 Canis lupus familiaris neurofascin (NFASC), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe45322 XM_005640782.2 Canis lupus familiaris neurofascin (NFASC), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe45314 XM_005640778.2 Canis lupus familiaris neurofascin (NFASC), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe45317 XM_005640780.2 Canis lupus familiaris neurofascin (NFASC), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe45321 XM_003434326.3 Canis lupus familiaris neurofascin (NFASC), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe31640 XM_005640783.2 Canis lupus familiaris neurofascin (NFASC), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe45309 XM_005640774.2 Canis lupus familiaris neurofascin (NFASC), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe45312 XM_014111177.1 Canis lupus familiaris neurofascin (NFASC), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe45319 XM_545682.5 Canis lupus familiaris neurofascin (NFASC), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe45320 XM_850983.4 Canis lupus familiaris neurofascin (NFASC), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe45311 XM_005640776.2 Canis lupus familiaris neurofascin (NFASC), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe45316 XM_851151.4 Canis lupus familiaris neurofascin (NFASC), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe45310 XM_005640775.2 Canis lupus familiaris neurofascin (NFASC), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe45315 XM_005640779.2 Canis lupus familiaris neurofascin (NFASC), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe285029
    Clone ID Related Accession (Same CDS sequence) XM_022415411.1
    Accession Version XM_022415411.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3423bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurofascin isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TACTCCAACA GCAGCTCCTC CCACGTTGCC CCCGACCACG
    GTGAGTGTGA CGGGCGTTGT GAGCAGTACA GACGCTACTG CCACCGCTGC CACCACCGAA
    GCCACAACAG TCCCCACCAT CCCAAGTGTC GCACCTACCA CCATGGCCAC CACCACCGTC
    GCCACAACTA CAACCACTGC CGCCACCACC ATGGAGAGGC CTCCCACCAC CACCTCCAAG
    ACTAAGATGC CCGAATCCGG CCCCAGAGCT GAGGAGGAAG AAGCTCAAGT TCCCAAATCA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022271119.1
    CDS304..3726
    Translation

    Target ORF information:

    RefSeq Version XM_022415411.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022415411.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TACTCCAACA GCAGCTCCTC CCACGTTGCC CCCGACCACG
    GTGAGTGTGA CGGGCGTTGT GAGCAGTACA GACGCTACTG CCACCGCTGC CACCACCGAA
    GCCACAACAG TCCCCACCAT CCCAAGTGTC GCACCTACCA CCATGGCCAC CACCACCGTC
    GCCACAACTA CAACCACTGC CGCCACCACC ATGGAGAGGC CTCCCACCAC CACCTCCAAG
    ACTAAGATGC CCGAATCCGG CCCCAGAGCT GAGGAGGAAG AAGCTCAAGT TCCCAAATCA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285030
    Clone ID Related Accession (Same CDS sequence) XM_022415412.1
    Accession Version XM_022415412.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurofascin isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TTACCCAGTG CCCCCAGACG TTTCCGAGTC CGGCAGCCCA ACCTGGAGAC AATCAACCTG
    GAATGGGATC ATCCGGAACA CCCCAATGGG ATCCTGATCG GATACACTCT CAAATATGTG
    GCTTTTAATG GAACCAAATT AGGAAAGCAG ATAGTGGAAA ACTTCTCTCC CAATCAGACC
    AAGTTCACGA TGCAAAGAGC AGACCCCGTG TCGCGCTACC GCTTTACCCT CAGTGCCAGG
    ACGCAGGTGG GCTCTGGGGA AGCAGTCACA GAGGAGTCAC CAGCACCCCC AAATGAAGCT
    TACACCAATA ACCAGGCAGA CATCGCCACC CAGGGCTGGT TCATTGGGCT CATGTGTGCC
    ATCGCCCTCC TGGTGCTGAT CCTGCTCATC GTCTGCTTCA TCAAGAGGAG TCGAGGTGGC
    AAATACCCAG TGCGAGAAAA GAAGGACGTT CCTCTTGGCC CCGAAGACCC CAAGGAAGAG
    GATGGCTCCT TTGACTACAG TGACGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACGTCC
    CTCGATGGTA CCATCAAGCA GCAAGAGAGC GACGACAGCC TGGTGGACTA TGGCGAGGGT
    GGCGAGGGGC AGTTCAATGA GGATGGGTCC TTCATCGGCC AGTACACGGT CAAAAAGGAC
    AAAGAGGAGA CAGAGGGCAA TGAAAGCTCA GAGGCCACAT CGCCTGTCAA CGCCATCTAT
    TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022271120.1
    CDS303..3554
    Translation

    Target ORF information:

    RefSeq Version XM_022415412.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022415412.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TTACCCAGTG CCCCCAGACG TTTCCGAGTC CGGCAGCCCA ACCTGGAGAC AATCAACCTG
    GAATGGGATC ATCCGGAACA CCCCAATGGG ATCCTGATCG GATACACTCT CAAATATGTG
    GCTTTTAATG GAACCAAATT AGGAAAGCAG ATAGTGGAAA ACTTCTCTCC CAATCAGACC
    AAGTTCACGA TGCAAAGAGC AGACCCCGTG TCGCGCTACC GCTTTACCCT CAGTGCCAGG
    ACGCAGGTGG GCTCTGGGGA AGCAGTCACA GAGGAGTCAC CAGCACCCCC AAATGAAGCT
    TACACCAATA ACCAGGCAGA CATCGCCACC CAGGGCTGGT TCATTGGGCT CATGTGTGCC
    ATCGCCCTCC TGGTGCTGAT CCTGCTCATC GTCTGCTTCA TCAAGAGGAG TCGAGGTGGC
    AAATACCCAG TGCGAGAAAA GAAGGACGTT CCTCTTGGCC CCGAAGACCC CAAGGAAGAG
    GATGGCTCCT TTGACTACAG TGACGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACGTCC
    CTCGATGGTA CCATCAAGCA GCAAGAGAGC GACGACAGCC TGGTGGACTA TGGCGAGGGT
    GGCGAGGGGC AGTTCAATGA GGATGGGTCC TTCATCGGCC AGTACACGGT CAAAAAGGAC
    AAAGAGGAGA CAGAGGGCAA TGAAAGCTCA GAGGCCACAT CGCCTGTCAA CGCCATCTAT
    TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285031
    Clone ID Related Accession (Same CDS sequence) XM_022415413.1
    Accession Version XM_022415413.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3201bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurofascin isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCACACG AGGAGTTGCA
    GAAAGGACAC CCAGCTTCAT GTATCCCCAG GGCACAGCGA GCAGTCAGAT GGTGCTGCGT
    GGCACGGATC TTCTGCTGGA GTGCATCGCC TCTGGGGTCC CCACGCCAGA CATCGCATGG
    TACAAGAAAG GTGGGGACCT CCCATCTGAC AAGGCCAAGT TTGAGAACTT TAACAAGGCT
    CTGCGTATCA CCAACGTCTC CGAGGAAGAC TCTGGGGAGT ATTTCTGCCT GGCCTCCAAC
    AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG
    GACGAACCCA AGAACCTTAT CCTGGCTCCC GGTGAGGACG GGAGACTGGT GTGTCGAGCC
    AATGGGAACC CCAAGCCCAC TGTCCAGTGG ATGGTAAATG GGGAACCACT GCAATCGGCA
    CCACCCAACC CAAACCGTGA GGTGGCTGGG GACACCATCA TCTTCCGGGA CACCCAGATC
    AGCAGCAGAG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT ACTGGCCAAC
    GCCTTCGTCA GCGTGCTGGA CGTGCCACCC CGGATGCTGT CACCCCGGAA CCAGCTCATT
    AGGGTGATCC TGTACAACCG GACACGGCTG GACTGCCCAT TCTTCGGGTC TCCCATCCCC
    ACACTCCGAT GGTTTAAGAA CGGGCAGGGA AGTAACCTGG ATGGTGGTAA CTACCACGTC
    TACGAGAATG GCAGTCTGGA AATCAAGATG ATCCGCAAAG AGGACCAAGG CATCTACACC
    TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAGAACCAGG TCCGCCTGGA GGTCAAAGAC
    CCCACCAGGA TCTACCGGAT GCCTGAAGAC CAGGTGGCCA AAAGGGGCAC GACGGTGCAG
    CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAGCTCA CGGTCTCCTG GCTGAAGGAT
    GATGAGCCTC TCTACATAGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT
    GGTGTGGCTG AGCGGGACCA GGGCAGTTAC ACATGTGTTG CCAGCACTGA GCTGGACCAG
    GACCTGGCCA AGGCCTACCT CACCGTGCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG
    GCTGCCCTGC CCAAAGGACG GCCAGACCGA CCCCGGGACT TGGAGCTCAC TGACCTGGCT
    GAGAGGAGTG TGAGGCTGAC CTGGATCCCA GGGGATGATA ACAACAGCCC CATCACAGAC
    TACGTCGTCC AGTTTGAAGA GGACCAGTTC CAGCCAGGGG TCTGGCATGA CCATTCCAAG
    TTCCCAGGCA GCGTCAACTC TGCCATCCTC CAGTTGTCCC CATATGTCAA CTACCAGTTC
    CGGGTCATCG CCATCAACGA GGTGGGCAGC AGCCATCCCA GCCTCCCCTC GGAGCGCTAC
    AGGACCAGCG GGGCGCCCCC TGAGTCCAAT CCCGCCGACG TAAAGGGAGA AGGGACCAGA
    AAGAACAACA TGGAGATCAC TTGGACGCCC ATGAACGCCA CCTCTGCCTT TGGCCCCAAC
    CTGCGCTACA TCGTCAAGTG GCGGCGGAGA GAAACTCGAG AGACGTGGAA CAACGCCACA
    GTGTGGGGCT CCCGCTACGT GGTGGGCCAG ACCCCTGTCT ACGTGCCCTA TGAGATCCGA
    GTCCAGGCCG AAAATGACTT TGGGAAGGGC CCTGAGCCAG ACACTGTCAT CGGTTACTCC
    GGGGAAGACT TACCCAGTGC CCCCAGACGT TTCCGAGTCC GGCAGCCCAA CCTGGAGACA
    ATCAACCTGG AATGGGATCA TCCGGAACAC CCCAATGGGA TCCTGATCGG ATACACTCTC
    AAATATGTGG CTTTTAATGG AACCAAATTA GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC
    AATCAGACCA AGTTCACGAT GCAAAGAGCA GACCCCGTGT CGCGCTACCG CTTTACCCTC
    AGTGCCAGGA CGCAGGTGGG CTCTGGGGAA GCAGTCACAG AGGAGTCACC AGCACCCCCA
    AATGAAGCTT ACACCAATAA CCAGGCAGAC ATCGCCACCC AGGGCTGGTT CATTGGGCTC
    ATGTGTGCCA TCGCCCTCCT GGTGCTGATC CTGCTCATCG TCTGCTTCAT CAAGAGGAGT
    CGAGGTGGCA AATACCCAGT GCGAGAAAAG AAGGACGTTC CTCTTGGCCC CGAAGACCCC
    AAGGAAGAGG ATGGCTCCTT TGACTACAGT GACGAGGACA ACAAGCCCCT GCAGGGCAGC
    CAGACGTCCC TCGATGGTAC CATCAAGCAG CAAGAGAGCG ACGACAGCCT GGTGGACTAT
    GGCGAGGGTG GCGAGGGGCA GTTCAATGAG GATGGGTCCT TCATCGGCCA GTACACGGTC
    AAAAAGGACA AAGAGGAGAC AGAGGGCAAT GAAAGCTCAG AGGCCACATC GCCTGTCAAC
    GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022271121.1
    CDS303..3503
    Translation

    Target ORF information:

    RefSeq Version XM_022415413.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022415413.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCACACG AGGAGTTGCA
    GAAAGGACAC CCAGCTTCAT GTATCCCCAG GGCACAGCGA GCAGTCAGAT GGTGCTGCGT
    GGCACGGATC TTCTGCTGGA GTGCATCGCC TCTGGGGTCC CCACGCCAGA CATCGCATGG
    TACAAGAAAG GTGGGGACCT CCCATCTGAC AAGGCCAAGT TTGAGAACTT TAACAAGGCT
    CTGCGTATCA CCAACGTCTC CGAGGAAGAC TCTGGGGAGT ATTTCTGCCT GGCCTCCAAC
    AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG
    GACGAACCCA AGAACCTTAT CCTGGCTCCC GGTGAGGACG GGAGACTGGT GTGTCGAGCC
    AATGGGAACC CCAAGCCCAC TGTCCAGTGG ATGGTAAATG GGGAACCACT GCAATCGGCA
    CCACCCAACC CAAACCGTGA GGTGGCTGGG GACACCATCA TCTTCCGGGA CACCCAGATC
    AGCAGCAGAG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT ACTGGCCAAC
    GCCTTCGTCA GCGTGCTGGA CGTGCCACCC CGGATGCTGT CACCCCGGAA CCAGCTCATT
    AGGGTGATCC TGTACAACCG GACACGGCTG GACTGCCCAT TCTTCGGGTC TCCCATCCCC
    ACACTCCGAT GGTTTAAGAA CGGGCAGGGA AGTAACCTGG ATGGTGGTAA CTACCACGTC
    TACGAGAATG GCAGTCTGGA AATCAAGATG ATCCGCAAAG AGGACCAAGG CATCTACACC
    TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAGAACCAGG TCCGCCTGGA GGTCAAAGAC
    CCCACCAGGA TCTACCGGAT GCCTGAAGAC CAGGTGGCCA AAAGGGGCAC GACGGTGCAG
    CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAGCTCA CGGTCTCCTG GCTGAAGGAT
    GATGAGCCTC TCTACATAGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT
    GGTGTGGCTG AGCGGGACCA GGGCAGTTAC ACATGTGTTG CCAGCACTGA GCTGGACCAG
    GACCTGGCCA AGGCCTACCT CACCGTGCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG
    GCTGCCCTGC CCAAAGGACG GCCAGACCGA CCCCGGGACT TGGAGCTCAC TGACCTGGCT
    GAGAGGAGTG TGAGGCTGAC CTGGATCCCA GGGGATGATA ACAACAGCCC CATCACAGAC
    TACGTCGTCC AGTTTGAAGA GGACCAGTTC CAGCCAGGGG TCTGGCATGA CCATTCCAAG
    TTCCCAGGCA GCGTCAACTC TGCCATCCTC CAGTTGTCCC CATATGTCAA CTACCAGTTC
    CGGGTCATCG CCATCAACGA GGTGGGCAGC AGCCATCCCA GCCTCCCCTC GGAGCGCTAC
    AGGACCAGCG GGGCGCCCCC TGAGTCCAAT CCCGCCGACG TAAAGGGAGA AGGGACCAGA
    AAGAACAACA TGGAGATCAC TTGGACGCCC ATGAACGCCA CCTCTGCCTT TGGCCCCAAC
    CTGCGCTACA TCGTCAAGTG GCGGCGGAGA GAAACTCGAG AGACGTGGAA CAACGCCACA
    GTGTGGGGCT CCCGCTACGT GGTGGGCCAG ACCCCTGTCT ACGTGCCCTA TGAGATCCGA
    GTCCAGGCCG AAAATGACTT TGGGAAGGGC CCTGAGCCAG ACACTGTCAT CGGTTACTCC
    GGGGAAGACT TACCCAGTGC CCCCAGACGT TTCCGAGTCC GGCAGCCCAA CCTGGAGACA
    ATCAACCTGG AATGGGATCA TCCGGAACAC CCCAATGGGA TCCTGATCGG ATACACTCTC
    AAATATGTGG CTTTTAATGG AACCAAATTA GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC
    AATCAGACCA AGTTCACGAT GCAAAGAGCA GACCCCGTGT CGCGCTACCG CTTTACCCTC
    AGTGCCAGGA CGCAGGTGGG CTCTGGGGAA GCAGTCACAG AGGAGTCACC AGCACCCCCA
    AATGAAGCTT ACACCAATAA CCAGGCAGAC ATCGCCACCC AGGGCTGGTT CATTGGGCTC
    ATGTGTGCCA TCGCCCTCCT GGTGCTGATC CTGCTCATCG TCTGCTTCAT CAAGAGGAGT
    CGAGGTGGCA AATACCCAGT GCGAGAAAAG AAGGACGTTC CTCTTGGCCC CGAAGACCCC
    AAGGAAGAGG ATGGCTCCTT TGACTACAGT GACGAGGACA ACAAGCCCCT GCAGGGCAGC
    CAGACGTCCC TCGATGGTAC CATCAAGCAG CAAGAGAGCG ACGACAGCCT GGTGGACTAT
    GGCGAGGGTG GCGAGGGGCA GTTCAATGAG GATGGGTCCT TCATCGGCCA GTACACGGTC
    AAAAAGGACA AAGAGGAGAC AGAGGGCAAT GAAAGCTCAG AGGCCACATC GCCTGTCAAC
    GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45309
    Clone ID Related Accession (Same CDS sequence) XM_005640774.2 , XM_005640774.3
    Accession Version XM_005640774.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neurofascin isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640774.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CTATCCCAGC CCCCAACCAT CACCAAGCAG
    TCAGTGAAGG ACCACATCGT GGATCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAG
    GGGAACCCTG CCCCCAGCTT CCACTGGACT CGAAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCAATGAG GAGAAAGTCT GGGACCCTGG TGATCGACTT CCGTAGTGGT
    GGGCGGCCCG AAGAATATGA AGGGGAATAC CAGTGCTTTG CCCGCAACAA ATTTGGCACG
    GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAGTCTC CCCTGTGGCC CAAGGAGAAC
    CTAGACCCAG TCGTGGTTCA AGAAGGTGCT CCTTTGACAC TCCAGTGCAA CCCTCCACCC
    GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACTCAGGAC
    AAGCGTGTCT CTCAGGGCCA CAACGGTGAC CTGTATTTCT CCAACGTGAT GCTTCAGGAT
    ATGCAGACAG ACTACAGCTG CAACGCGCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC
    CACCCTGACA TGTACAGTGC ACGAGGAGTT GCAGAAAGGA CACCCAGCTT CATGTATCCC
    CAGGGCACAG CGAGCAGTCA GATGGTGCTG CGTGGCACGG ATCTTCTGCT GGAGTGCATC
    GCCTCTGGGG TCCCCACGCC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT
    GACAAGGCCA AGTTTGAGAA CTTTAACAAG GCTCTGCGTA TCACCAACGT CTCCGAGGAA
    GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC
    TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATCCTGGCT
    CCCGGTGAGG ACGGGAGACT GGTGTGTCGA GCCAATGGGA ACCCCAAGCC CACTGTCCAG
    TGGATGGTAA ATGGGGAACC ACTGCAATCG GCACCACCCA ACCCAAACCG TGAGGTGGCT
    GGGGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GAGCTGTGTA CCAGTGCAAC
    ACCTCCAACG AGCATGGCTA CCTACTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCA
    CCCCGGATGC TGTCACCCCG GAACCAGCTC ATTAGGGTGA TCCTGTACAA CCGGACACGG
    CTGGACTGCC CATTCTTCGG GTCTCCCATC CCCACACTCC GATGGTTTAA GAACGGGCAG
    GGAAGTAACC TGGATGGTGG TAACTACCAC GTCTACGAGA ATGGCAGTCT GGAAATCAAG
    ATGATCCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA
    GCTGAGAACC AGGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCTGAA
    GACCAGGTGG CCAAAAGGGG CACGACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC
    TCCCTGAAGC TCACGGTCTC CTGGCTGAAG GATGATGAGC CTCTCTACAT AGGAAACAGG
    ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGTGTGG CTGAGCGGGA CCAGGGCAGT
    TACACATGTG TTGCCAGCAC TGAGCTGGAC CAGGACCTGG CCAAGGCCTA CCTCACCGTG
    CTAGCTGATC AGGCCACTCC AACTAACCGT TTGGCTGCCC TGCCCAAAGG ACGGCCAGAC
    CGACCCCGGG ACTTGGAGCT CACTGACCTG GCTGAGAGGA GTGTGAGGCT GACCTGGATC
    CCAGGGGATG ATAACAACAG CCCCATCACA GACTACGTCG TCCAGTTTGA AGAGGACCAG
    TTCCAGCCAG GGGTCTGGCA TGACCATTCC AAGTTCCCAG GCAGCGTCAA CTCTGCCATC
    CTCCAGTTGT CCCCATATGT CAACTACCAG TTCCGGGTCA TCGCCATCAA CGAGGTGGGC
    AGCAGCCATC CCAGCCTCCC CTCGGAGCGC TACAGGACCA GCGGGGCGCC CCCTGAGTCC
    AATCCCGCCG ACGTAAAGGG AGAAGGGACC AGAAAGAACA ACATGGAGAT CACTTGGACG
    CCCATGAACG CCACCTCTGC CTTTGGCCCC AACCTGCGCT ACATCGTCAA GTGGCGGCGG
    AGAGAAACTC GAGAGACGTG GAACAACGCC ACAGTGTGGG GCTCCCGCTA CGTGGTGGGC
    CAGACCCCTG TCTACGTGCC CTATGAGATC CGAGTCCAGG CCGAAAATGA CTTTGGGAAG
    GGCCCTGAGC CAGACACTGT CATCGGTTAC TCCGGGGAAG ACTATCCCAG GGCTGCACCC
    ACTGACGTTA AAATCCGAGT CCTGAACAGC ACAGCCATCA GTCTCCAGTG GAACCGCGTT
    TACCCTGACA CAGTCCAGGG CCAGCTCAGA GAGTACCGAG CCTACTACTG GAGGGAGAGC
    AGCTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AAGCCAGCTT CCCTGGTGAC
    CGCCCCCGCG GCGTGGTGTC CCGCCTCTTC CCCTACAGTA ACTACAAGCT GGAGATGGTC
    GTGGTCAATG GGAGAGGCGA TGGGCCTCGC AGTGAAACCA AGGAGTTCAC CACCCCGGAA
    GGAGTACCCA GTGCCCCCAG ACGTTTCCGA GTCCGGCAGC CCAACCTGGA GACAATCAAC
    CTGGAATGGG ATCATCCGGA ACACCCCAAT GGGATCCTGA TCGGATACAC TCTCAAATAT
    GTGGCTTTTA ATGGAACCAA ATTAGGAAAG CAGATAGTGG AAAACTTCTC TCCCAATCAG
    ACCAAGTTCA CGATGCAAAG AGCAGACCCC GTGTCGCGCT ACCGCTTTAC CCTCAGTGCC
    AGGACGCAGG TGGGCTCTGG GGAAGCAGTC ACAGAGGAGT CACCAGCACC CCCAAATGAA
    GCTACTCCAA CAGCAGCTCC TCCCACGTTG CCCCCGACCA CGGTGAGTGT GACGGGCGTT
    GTGAGCAGTA CAGACGCTAC TGCCACCGCT GCCACCACCG AAGCCACAAC AGTCCCCACC
    ATCCCAAGTG TCGCACCTAC CACCATGGCC ACCACCACCG TCGCCACAAC TACAACCACT
    GCCGCCACCA CCATGGAGAG GCCTCCCACC ACCACCTCCA AGACTAAGAT GCCCGAATCC
    GCCCCCGACA GGCAGTCCAT ATGGAACGTC ACGGTGCTCC CCAACAGTAA ATGGGCCAAC
    ATCACCTGGA GACACAATCT CGGGCCCGGA ACTGCCTTTG TGGTTGAGTA CATCGACAGC
    AACCATACGA AAAAAACTGT CCCTGTTAAG AGCCAGGTCC AGCCCGTACA GCTGACAGAC
    CTCTATCCCG GGATGACATA CACGTTGCGC GTTTATTCCC GGGACAAGGA GGGCATCAGC
    AGTACCGTCA TCACCTTTAT GACCAGTACA GCTTACACCA ATAACCAGGC AGACATCGCC
    ACCCAGGGCT GGTTCATTGG GCTCATGTGT GCCATCGCCC TCCTGGTGCT GATCCTGCTC
    ATCGTCTGCT TCATCAAGAG GAGTCGAGGT GGCAAATACC CAGTGCGAGA AAAGAAGGAC
    GTTCCTCTTG GCCCCGAAGA CCCCAAGGAA GAGGATGGCT CCTTTGACTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTCGATG GTACCATCAA GCAGCAAGAG
    AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GGCAGTTCAA TGAGGATGGG
    TCCTTCATCG GCCAGTACAC GGTCAAAAAG GACAAAGAGG AGACAGAGGG CAATGAAAGC
    TCAGAGGCCA CATCGCCTGT CAACGCCATC TATTCTCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640831.2
    CDS288..4352
    Translation

    Target ORF information:

    RefSeq Version XM_005640774.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640774.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CTATCCCAGC CCCCAACCAT CACCAAGCAG
    TCAGTGAAGG ACCACATCGT GGATCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAG
    GGGAACCCTG CCCCCAGCTT CCACTGGACT CGAAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCAATGAG GAGAAAGTCT GGGACCCTGG TGATCGACTT CCGTAGTGGT
    GGGCGGCCCG AAGAATATGA AGGGGAATAC CAGTGCTTTG CCCGCAACAA ATTTGGCACG
    GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAGTCTC CCCTGTGGCC CAAGGAGAAC
    CTAGACCCAG TCGTGGTTCA AGAAGGTGCT CCTTTGACAC TCCAGTGCAA CCCTCCACCC
    GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACTCAGGAC
    AAGCGTGTCT CTCAGGGCCA CAACGGTGAC CTGTATTTCT CCAACGTGAT GCTTCAGGAT
    ATGCAGACAG ACTACAGCTG CAACGCGCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC
    CACCCTGACA TGTACAGTGC ACGAGGAGTT GCAGAAAGGA CACCCAGCTT CATGTATCCC
    CAGGGCACAG CGAGCAGTCA GATGGTGCTG CGTGGCACGG ATCTTCTGCT GGAGTGCATC
    GCCTCTGGGG TCCCCACGCC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT
    GACAAGGCCA AGTTTGAGAA CTTTAACAAG GCTCTGCGTA TCACCAACGT CTCCGAGGAA
    GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC
    TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATCCTGGCT
    CCCGGTGAGG ACGGGAGACT GGTGTGTCGA GCCAATGGGA ACCCCAAGCC CACTGTCCAG
    TGGATGGTAA ATGGGGAACC ACTGCAATCG GCACCACCCA ACCCAAACCG TGAGGTGGCT
    GGGGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GAGCTGTGTA CCAGTGCAAC
    ACCTCCAACG AGCATGGCTA CCTACTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCA
    CCCCGGATGC TGTCACCCCG GAACCAGCTC ATTAGGGTGA TCCTGTACAA CCGGACACGG
    CTGGACTGCC CATTCTTCGG GTCTCCCATC CCCACACTCC GATGGTTTAA GAACGGGCAG
    GGAAGTAACC TGGATGGTGG TAACTACCAC GTCTACGAGA ATGGCAGTCT GGAAATCAAG
    ATGATCCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA
    GCTGAGAACC AGGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCTGAA
    GACCAGGTGG CCAAAAGGGG CACGACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC
    TCCCTGAAGC TCACGGTCTC CTGGCTGAAG GATGATGAGC CTCTCTACAT AGGAAACAGG
    ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGTGTGG CTGAGCGGGA CCAGGGCAGT
    TACACATGTG TTGCCAGCAC TGAGCTGGAC CAGGACCTGG CCAAGGCCTA CCTCACCGTG
    CTAGCTGATC AGGCCACTCC AACTAACCGT TTGGCTGCCC TGCCCAAAGG ACGGCCAGAC
    CGACCCCGGG ACTTGGAGCT CACTGACCTG GCTGAGAGGA GTGTGAGGCT GACCTGGATC
    CCAGGGGATG ATAACAACAG CCCCATCACA GACTACGTCG TCCAGTTTGA AGAGGACCAG
    TTCCAGCCAG GGGTCTGGCA TGACCATTCC AAGTTCCCAG GCAGCGTCAA CTCTGCCATC
    CTCCAGTTGT CCCCATATGT CAACTACCAG TTCCGGGTCA TCGCCATCAA CGAGGTGGGC
    AGCAGCCATC CCAGCCTCCC CTCGGAGCGC TACAGGACCA GCGGGGCGCC CCCTGAGTCC
    AATCCCGCCG ACGTAAAGGG AGAAGGGACC AGAAAGAACA ACATGGAGAT CACTTGGACG
    CCCATGAACG CCACCTCTGC CTTTGGCCCC AACCTGCGCT ACATCGTCAA GTGGCGGCGG
    AGAGAAACTC GAGAGACGTG GAACAACGCC ACAGTGTGGG GCTCCCGCTA CGTGGTGGGC
    CAGACCCCTG TCTACGTGCC CTATGAGATC CGAGTCCAGG CCGAAAATGA CTTTGGGAAG
    GGCCCTGAGC CAGACACTGT CATCGGTTAC TCCGGGGAAG ACTATCCCAG GGCTGCACCC
    ACTGACGTTA AAATCCGAGT CCTGAACAGC ACAGCCATCA GTCTCCAGTG GAACCGCGTT
    TACCCTGACA CAGTCCAGGG CCAGCTCAGA GAGTACCGAG CCTACTACTG GAGGGAGAGC
    AGCTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AAGCCAGCTT CCCTGGTGAC
    CGCCCCCGCG GCGTGGTGTC CCGCCTCTTC CCCTACAGTA ACTACAAGCT GGAGATGGTC
    GTGGTCAATG GGAGAGGCGA TGGGCCTCGC AGTGAAACCA AGGAGTTCAC CACCCCGGAA
    GGAGTACCCA GTGCCCCCAG ACGTTTCCGA GTCCGGCAGC CCAACCTGGA GACAATCAAC
    CTGGAATGGG ATCATCCGGA ACACCCCAAT GGGATCCTGA TCGGATACAC TCTCAAATAT
    GTGGCTTTTA ATGGAACCAA ATTAGGAAAG CAGATAGTGG AAAACTTCTC TCCCAATCAG
    ACCAAGTTCA CGATGCAAAG AGCAGACCCC GTGTCGCGCT ACCGCTTTAC CCTCAGTGCC
    AGGACGCAGG TGGGCTCTGG GGAAGCAGTC ACAGAGGAGT CACCAGCACC CCCAAATGAA
    GCTACTCCAA CAGCAGCTCC TCCCACGTTG CCCCCGACCA CGGTGAGTGT GACGGGCGTT
    GTGAGCAGTA CAGACGCTAC TGCCACCGCT GCCACCACCG AAGCCACAAC AGTCCCCACC
    ATCCCAAGTG TCGCACCTAC CACCATGGCC ACCACCACCG TCGCCACAAC TACAACCACT
    GCCGCCACCA CCATGGAGAG GCCTCCCACC ACCACCTCCA AGACTAAGAT GCCCGAATCC
    GCCCCCGACA GGCAGTCCAT ATGGAACGTC ACGGTGCTCC CCAACAGTAA ATGGGCCAAC
    ATCACCTGGA GACACAATCT CGGGCCCGGA ACTGCCTTTG TGGTTGAGTA CATCGACAGC
    AACCATACGA AAAAAACTGT CCCTGTTAAG AGCCAGGTCC AGCCCGTACA GCTGACAGAC
    CTCTATCCCG GGATGACATA CACGTTGCGC GTTTATTCCC GGGACAAGGA GGGCATCAGC
    AGTACCGTCA TCACCTTTAT GACCAGTACA GCTTACACCA ATAACCAGGC AGACATCGCC
    ACCCAGGGCT GGTTCATTGG GCTCATGTGT GCCATCGCCC TCCTGGTGCT GATCCTGCTC
    ATCGTCTGCT TCATCAAGAG GAGTCGAGGT GGCAAATACC CAGTGCGAGA AAAGAAGGAC
    GTTCCTCTTG GCCCCGAAGA CCCCAAGGAA GAGGATGGCT CCTTTGACTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTCGATG GTACCATCAA GCAGCAAGAG
    AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GGCAGTTCAA TGAGGATGGG
    TCCTTCATCG GCCAGTACAC GGTCAAAAAG GACAAAGAGG AGACAGAGGG CAATGAAAGC
    TCAGAGGCCA CATCGCCTGT CAACGCCATC TATTCTCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45309
    Clone ID Related Accession (Same CDS sequence) XM_005640774.2 , XM_005640774.3
    Accession Version XM_005640774.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurofascin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005640774.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CTATCCCAGC CCCCAACCAT CACCAAGCAG
    TCAGTGAAGG ACCACATCGT GGATCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAG
    GGGAACCCTG CCCCCAGCTT CCACTGGACT CGAAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCAATGAG GAGAAAGTCT GGGACCCTGG TGATCGACTT CCGTAGTGGT
    GGGCGGCCCG AAGAATATGA AGGGGAATAC CAGTGCTTTG CCCGCAACAA ATTTGGCACG
    GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAGTCTC CCCTGTGGCC CAAGGAGAAC
    CTAGACCCAG TCGTGGTTCA AGAAGGTGCT CCTTTGACAC TCCAGTGCAA CCCTCCACCC
    GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACTCAGGAC
    AAGCGTGTCT CTCAGGGCCA CAACGGTGAC CTGTATTTCT CCAACGTGAT GCTTCAGGAT
    ATGCAGACAG ACTACAGCTG CAACGCGCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC
    CACCCTGACA TGTACAGTGC ACGAGGAGTT GCAGAAAGGA CACCCAGCTT CATGTATCCC
    CAGGGCACAG CGAGCAGTCA GATGGTGCTG CGTGGCACGG ATCTTCTGCT GGAGTGCATC
    GCCTCTGGGG TCCCCACGCC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT
    GACAAGGCCA AGTTTGAGAA CTTTAACAAG GCTCTGCGTA TCACCAACGT CTCCGAGGAA
    GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC
    TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATCCTGGCT
    CCCGGTGAGG ACGGGAGACT GGTGTGTCGA GCCAATGGGA ACCCCAAGCC CACTGTCCAG
    TGGATGGTAA ATGGGGAACC ACTGCAATCG GCACCACCCA ACCCAAACCG TGAGGTGGCT
    GGGGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GAGCTGTGTA CCAGTGCAAC
    ACCTCCAACG AGCATGGCTA CCTACTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCA
    CCCCGGATGC TGTCACCCCG GAACCAGCTC ATTAGGGTGA TCCTGTACAA CCGGACACGG
    CTGGACTGCC CATTCTTCGG GTCTCCCATC CCCACACTCC GATGGTTTAA GAACGGGCAG
    GGAAGTAACC TGGATGGTGG TAACTACCAC GTCTACGAGA ATGGCAGTCT GGAAATCAAG
    ATGATCCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA
    GCTGAGAACC AGGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCTGAA
    GACCAGGTGG CCAAAAGGGG CACGACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC
    TCCCTGAAGC TCACGGTCTC CTGGCTGAAG GATGATGAGC CTCTCTACAT AGGAAACAGG
    ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGTGTGG CTGAGCGGGA CCAGGGCAGT
    TACACATGTG TTGCCAGCAC TGAGCTGGAC CAGGACCTGG CCAAGGCCTA CCTCACCGTG
    CTAGCTGATC AGGCCACTCC AACTAACCGT TTGGCTGCCC TGCCCAAAGG ACGGCCAGAC
    CGACCCCGGG ACTTGGAGCT CACTGACCTG GCTGAGAGGA GTGTGAGGCT GACCTGGATC
    CCAGGGGATG ATAACAACAG CCCCATCACA GACTACGTCG TCCAGTTTGA AGAGGACCAG
    TTCCAGCCAG GGGTCTGGCA TGACCATTCC AAGTTCCCAG GCAGCGTCAA CTCTGCCATC
    CTCCAGTTGT CCCCATATGT CAACTACCAG TTCCGGGTCA TCGCCATCAA CGAGGTGGGC
    AGCAGCCATC CCAGCCTCCC CTCGGAGCGC TACAGGACCA GCGGGGCGCC CCCTGAGTCC
    AATCCCGCCG ACGTAAAGGG AGAAGGGACC AGAAAGAACA ACATGGAGAT CACTTGGACG
    CCCATGAACG CCACCTCTGC CTTTGGCCCC AACCTGCGCT ACATCGTCAA GTGGCGGCGG
    AGAGAAACTC GAGAGACGTG GAACAACGCC ACAGTGTGGG GCTCCCGCTA CGTGGTGGGC
    CAGACCCCTG TCTACGTGCC CTATGAGATC CGAGTCCAGG CCGAAAATGA CTTTGGGAAG
    GGCCCTGAGC CAGACACTGT CATCGGTTAC TCCGGGGAAG ACTATCCCAG GGCTGCACCC
    ACTGACGTTA AAATCCGAGT CCTGAACAGC ACAGCCATCA GTCTCCAGTG GAACCGCGTT
    TACCCTGACA CAGTCCAGGG CCAGCTCAGA GAGTACCGAG CCTACTACTG GAGGGAGAGC
    AGCTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AAGCCAGCTT CCCTGGTGAC
    CGCCCCCGCG GCGTGGTGTC CCGCCTCTTC CCCTACAGTA ACTACAAGCT GGAGATGGTC
    GTGGTCAATG GGAGAGGCGA TGGGCCTCGC AGTGAAACCA AGGAGTTCAC CACCCCGGAA
    GGAGTACCCA GTGCCCCCAG ACGTTTCCGA GTCCGGCAGC CCAACCTGGA GACAATCAAC
    CTGGAATGGG ATCATCCGGA ACACCCCAAT GGGATCCTGA TCGGATACAC TCTCAAATAT
    GTGGCTTTTA ATGGAACCAA ATTAGGAAAG CAGATAGTGG AAAACTTCTC TCCCAATCAG
    ACCAAGTTCA CGATGCAAAG AGCAGACCCC GTGTCGCGCT ACCGCTTTAC CCTCAGTGCC
    AGGACGCAGG TGGGCTCTGG GGAAGCAGTC ACAGAGGAGT CACCAGCACC CCCAAATGAA
    GCTACTCCAA CAGCAGCTCC TCCCACGTTG CCCCCGACCA CGGTGAGTGT GACGGGCGTT
    GTGAGCAGTA CAGACGCTAC TGCCACCGCT GCCACCACCG AAGCCACAAC AGTCCCCACC
    ATCCCAAGTG TCGCACCTAC CACCATGGCC ACCACCACCG TCGCCACAAC TACAACCACT
    GCCGCCACCA CCATGGAGAG GCCTCCCACC ACCACCTCCA AGACTAAGAT GCCCGAATCC
    GCCCCCGACA GGCAGTCCAT ATGGAACGTC ACGGTGCTCC CCAACAGTAA ATGGGCCAAC
    ATCACCTGGA GACACAATCT CGGGCCCGGA ACTGCCTTTG TGGTTGAGTA CATCGACAGC
    AACCATACGA AAAAAACTGT CCCTGTTAAG AGCCAGGTCC AGCCCGTACA GCTGACAGAC
    CTCTATCCCG GGATGACATA CACGTTGCGC GTTTATTCCC GGGACAAGGA GGGCATCAGC
    AGTACCGTCA TCACCTTTAT GACCAGTACA GCTTACACCA ATAACCAGGC AGACATCGCC
    ACCCAGGGCT GGTTCATTGG GCTCATGTGT GCCATCGCCC TCCTGGTGCT GATCCTGCTC
    ATCGTCTGCT TCATCAAGAG GAGTCGAGGT GGCAAATACC CAGTGCGAGA AAAGAAGGAC
    GTTCCTCTTG GCCCCGAAGA CCCCAAGGAA GAGGATGGCT CCTTTGACTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTCGATG GTACCATCAA GCAGCAAGAG
    AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GGCAGTTCAA TGAGGATGGG
    TCCTTCATCG GCCAGTACAC GGTCAAAAAG GACAAAGAGG AGACAGAGGG CAATGAAAGC
    TCAGAGGCCA CATCGCCTGT CAACGCCATC TATTCTCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640831.2
    CDS304..4368
    Translation

    Target ORF information:

    RefSeq Version XM_005640774.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640774.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CTATCCCAGC CCCCAACCAT CACCAAGCAG
    TCAGTGAAGG ACCACATCGT GGATCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAG
    GGGAACCCTG CCCCCAGCTT CCACTGGACT CGAAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCAATGAG GAGAAAGTCT GGGACCCTGG TGATCGACTT CCGTAGTGGT
    GGGCGGCCCG AAGAATATGA AGGGGAATAC CAGTGCTTTG CCCGCAACAA ATTTGGCACG
    GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAGTCTC CCCTGTGGCC CAAGGAGAAC
    CTAGACCCAG TCGTGGTTCA AGAAGGTGCT CCTTTGACAC TCCAGTGCAA CCCTCCACCC
    GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACTCAGGAC
    AAGCGTGTCT CTCAGGGCCA CAACGGTGAC CTGTATTTCT CCAACGTGAT GCTTCAGGAT
    ATGCAGACAG ACTACAGCTG CAACGCGCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC
    CACCCTGACA TGTACAGTGC ACGAGGAGTT GCAGAAAGGA CACCCAGCTT CATGTATCCC
    CAGGGCACAG CGAGCAGTCA GATGGTGCTG CGTGGCACGG ATCTTCTGCT GGAGTGCATC
    GCCTCTGGGG TCCCCACGCC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT
    GACAAGGCCA AGTTTGAGAA CTTTAACAAG GCTCTGCGTA TCACCAACGT CTCCGAGGAA
    GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC
    TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATCCTGGCT
    CCCGGTGAGG ACGGGAGACT GGTGTGTCGA GCCAATGGGA ACCCCAAGCC CACTGTCCAG
    TGGATGGTAA ATGGGGAACC ACTGCAATCG GCACCACCCA ACCCAAACCG TGAGGTGGCT
    GGGGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GAGCTGTGTA CCAGTGCAAC
    ACCTCCAACG AGCATGGCTA CCTACTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCA
    CCCCGGATGC TGTCACCCCG GAACCAGCTC ATTAGGGTGA TCCTGTACAA CCGGACACGG
    CTGGACTGCC CATTCTTCGG GTCTCCCATC CCCACACTCC GATGGTTTAA GAACGGGCAG
    GGAAGTAACC TGGATGGTGG TAACTACCAC GTCTACGAGA ATGGCAGTCT GGAAATCAAG
    ATGATCCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA
    GCTGAGAACC AGGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCTGAA
    GACCAGGTGG CCAAAAGGGG CACGACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC
    TCCCTGAAGC TCACGGTCTC CTGGCTGAAG GATGATGAGC CTCTCTACAT AGGAAACAGG
    ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGTGTGG CTGAGCGGGA CCAGGGCAGT
    TACACATGTG TTGCCAGCAC TGAGCTGGAC CAGGACCTGG CCAAGGCCTA CCTCACCGTG
    CTAGCTGATC AGGCCACTCC AACTAACCGT TTGGCTGCCC TGCCCAAAGG ACGGCCAGAC
    CGACCCCGGG ACTTGGAGCT CACTGACCTG GCTGAGAGGA GTGTGAGGCT GACCTGGATC
    CCAGGGGATG ATAACAACAG CCCCATCACA GACTACGTCG TCCAGTTTGA AGAGGACCAG
    TTCCAGCCAG GGGTCTGGCA TGACCATTCC AAGTTCCCAG GCAGCGTCAA CTCTGCCATC
    CTCCAGTTGT CCCCATATGT CAACTACCAG TTCCGGGTCA TCGCCATCAA CGAGGTGGGC
    AGCAGCCATC CCAGCCTCCC CTCGGAGCGC TACAGGACCA GCGGGGCGCC CCCTGAGTCC
    AATCCCGCCG ACGTAAAGGG AGAAGGGACC AGAAAGAACA ACATGGAGAT CACTTGGACG
    CCCATGAACG CCACCTCTGC CTTTGGCCCC AACCTGCGCT ACATCGTCAA GTGGCGGCGG
    AGAGAAACTC GAGAGACGTG GAACAACGCC ACAGTGTGGG GCTCCCGCTA CGTGGTGGGC
    CAGACCCCTG TCTACGTGCC CTATGAGATC CGAGTCCAGG CCGAAAATGA CTTTGGGAAG
    GGCCCTGAGC CAGACACTGT CATCGGTTAC TCCGGGGAAG ACTATCCCAG GGCTGCACCC
    ACTGACGTTA AAATCCGAGT CCTGAACAGC ACAGCCATCA GTCTCCAGTG GAACCGCGTT
    TACCCTGACA CAGTCCAGGG CCAGCTCAGA GAGTACCGAG CCTACTACTG GAGGGAGAGC
    AGCTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AAGCCAGCTT CCCTGGTGAC
    CGCCCCCGCG GCGTGGTGTC CCGCCTCTTC CCCTACAGTA ACTACAAGCT GGAGATGGTC
    GTGGTCAATG GGAGAGGCGA TGGGCCTCGC AGTGAAACCA AGGAGTTCAC CACCCCGGAA
    GGAGTACCCA GTGCCCCCAG ACGTTTCCGA GTCCGGCAGC CCAACCTGGA GACAATCAAC
    CTGGAATGGG ATCATCCGGA ACACCCCAAT GGGATCCTGA TCGGATACAC TCTCAAATAT
    GTGGCTTTTA ATGGAACCAA ATTAGGAAAG CAGATAGTGG AAAACTTCTC TCCCAATCAG
    ACCAAGTTCA CGATGCAAAG AGCAGACCCC GTGTCGCGCT ACCGCTTTAC CCTCAGTGCC
    AGGACGCAGG TGGGCTCTGG GGAAGCAGTC ACAGAGGAGT CACCAGCACC CCCAAATGAA
    GCTACTCCAA CAGCAGCTCC TCCCACGTTG CCCCCGACCA CGGTGAGTGT GACGGGCGTT
    GTGAGCAGTA CAGACGCTAC TGCCACCGCT GCCACCACCG AAGCCACAAC AGTCCCCACC
    ATCCCAAGTG TCGCACCTAC CACCATGGCC ACCACCACCG TCGCCACAAC TACAACCACT
    GCCGCCACCA CCATGGAGAG GCCTCCCACC ACCACCTCCA AGACTAAGAT GCCCGAATCC
    GCCCCCGACA GGCAGTCCAT ATGGAACGTC ACGGTGCTCC CCAACAGTAA ATGGGCCAAC
    ATCACCTGGA GACACAATCT CGGGCCCGGA ACTGCCTTTG TGGTTGAGTA CATCGACAGC
    AACCATACGA AAAAAACTGT CCCTGTTAAG AGCCAGGTCC AGCCCGTACA GCTGACAGAC
    CTCTATCCCG GGATGACATA CACGTTGCGC GTTTATTCCC GGGACAAGGA GGGCATCAGC
    AGTACCGTCA TCACCTTTAT GACCAGTACA GCTTACACCA ATAACCAGGC AGACATCGCC
    ACCCAGGGCT GGTTCATTGG GCTCATGTGT GCCATCGCCC TCCTGGTGCT GATCCTGCTC
    ATCGTCTGCT TCATCAAGAG GAGTCGAGGT GGCAAATACC CAGTGCGAGA AAAGAAGGAC
    GTTCCTCTTG GCCCCGAAGA CCCCAAGGAA GAGGATGGCT CCTTTGACTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTCGATG GTACCATCAA GCAGCAAGAG
    AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GGCAGTTCAA TGAGGATGGG
    TCCTTCATCG GCCAGTACAC GGTCAAAAAG GACAAAGAGG AGACAGAGGG CAATGAAAGC
    TCAGAGGCCA CATCGCCTGT CAACGCCATC TATTCTCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45310
    Clone ID Related Accession (Same CDS sequence) XM_005640775.2 , XM_005640775.3
    Accession Version XM_005640775.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neurofascin isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640775.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGGACGGCCA GACCGACCCC GGGACTTGGA GCTCACTGAC
    CTGGCTGAGA GGAGTGTGAG GCTGACCTGG ATCCCAGGGG ATGATAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CAGGCAGCGT CAACTCTGCC ATCCTCCAGT TGTCCCCATA TGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAACGAGGTG GGCAGCAGCC ATCCCAGCCT CCCCTCGGAG
    CGCTACAGGA CCAGCGGGGC GCCCCCTGAG TCCAATCCCG CCGACGTAAA GGGAGAAGGG
    ACCAGAAAGA ACAACATGGA GATCACTTGG ACGCCCATGA ACGCCACCTC TGCCTTTGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAAA CTCGAGAGAC GTGGAACAAC
    GCCACAGTGT GGGGCTCCCG CTACGTGGTG GGCCAGACCC CTGTCTACGT GCCCTATGAG
    ATCCGAGTCC AGGCCGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCCGGGG AAGACTATCC CAGGGCTGCA CCCACTGACG TTAAAATCCG AGTCCTGAAC
    AGCACAGCCA TCAGTCTCCA GTGGAACCGC GTTTACCCTG ACACAGTCCA GGGCCAGCTC
    AGAGAGTACC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGAC AGCAAGCCAG CTTCCCTGGT GACCGCCCCC GCGGCGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTCGTGGTCA ATGGGAGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGACGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC GGAACACCCC
    AATGGGATCC TGATCGGATA CACTCTCAAA TATGTGGCTT TTAATGGAAC CAAATTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGATGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TACCCTCAGT GCCAGGACGC AGGTGGGCTC TGGGGAAGCA
    GTCACAGAGG AGTCACCAGC ACCCCCAAAT GAAGCTACTC CAACAGCAGC TCCTCCCACG
    TTGCCCCCGA CCACGGTGAG TGTGACGGGC GTTGTGAGCA GTACAGACGC TACTGCCACC
    GCTGCCACCA CCGAAGCCAC AACAGTCCCC ACCATCCCAA GTGTCGCACC TACCACCATG
    GCCACCACCA CCGTCGCCAC AACTACAACC ACTGCCGCCA CCACCATGGA GAGGCCTCCC
    ACCACCACCT CCAAGACTAA GATGCCCGAA TCCGCCCCCG ACAGGCAGTC CATATGGAAC
    GTCACGGTGC TCCCCAACAG TAAATGGGCC AACATCACCT GGAGACACAA TCTCGGGCCC
    GGAACTGCCT TTGTGGTTGA GTACATCGAC AGCAACCATA CGAAAAAAAC TGTCCCTGTT
    AAGAGCCAGG TCCAGCCCGT ACAGCTGACA GACCTCTATC CCGGGATGAC ATACACGTTG
    CGCGTTTATT CCCGGGACAA GGAGGGCATC AGCAGTACCG TCATCACCTT TATGACCAGT
    ACAGCTTACA CCAATAACCA GGCAGACATC GCCACCCAGG GCTGGTTCAT TGGGCTCATG
    TGTGCCATCG CCCTCCTGGT GCTGATCCTG CTCATCGTCT GCTTCATCAA GAGGAGTCGA
    GGTGGCAAAT ACCCAGTGCG AGAAAAGAAG GACGTTCCTC TTGGCCCCGA AGACCCCAAG
    GAAGAGGATG GCTCCTTTGA CTACAGTGAC GAGGACAACA AGCCCCTGCA GGGCAGCCAG
    ACGTCCCTCG ATGGTACCAT CAAGCAGCAA GAGAGCGACG ACAGCCTGGT GGACTATGGC
    GAGGGTGGCG AGGGGCAGTT CAATGAGGAT GGGTCCTTCA TCGGCCAGTA CACGGTCAAA
    AAGGACAAAG AGGAGACAGA GGGCAATGAA AGCTCAGAGG CCACATCGCC TGTCAACGCC
    ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640832.2
    CDS288..4325
    Translation

    Target ORF information:

    RefSeq Version XM_005640775.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640775.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGGACGGCCA GACCGACCCC GGGACTTGGA GCTCACTGAC
    CTGGCTGAGA GGAGTGTGAG GCTGACCTGG ATCCCAGGGG ATGATAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CAGGCAGCGT CAACTCTGCC ATCCTCCAGT TGTCCCCATA TGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAACGAGGTG GGCAGCAGCC ATCCCAGCCT CCCCTCGGAG
    CGCTACAGGA CCAGCGGGGC GCCCCCTGAG TCCAATCCCG CCGACGTAAA GGGAGAAGGG
    ACCAGAAAGA ACAACATGGA GATCACTTGG ACGCCCATGA ACGCCACCTC TGCCTTTGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAAA CTCGAGAGAC GTGGAACAAC
    GCCACAGTGT GGGGCTCCCG CTACGTGGTG GGCCAGACCC CTGTCTACGT GCCCTATGAG
    ATCCGAGTCC AGGCCGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCCGGGG AAGACTATCC CAGGGCTGCA CCCACTGACG TTAAAATCCG AGTCCTGAAC
    AGCACAGCCA TCAGTCTCCA GTGGAACCGC GTTTACCCTG ACACAGTCCA GGGCCAGCTC
    AGAGAGTACC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGAC AGCAAGCCAG CTTCCCTGGT GACCGCCCCC GCGGCGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTCGTGGTCA ATGGGAGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGACGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC GGAACACCCC
    AATGGGATCC TGATCGGATA CACTCTCAAA TATGTGGCTT TTAATGGAAC CAAATTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGATGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TACCCTCAGT GCCAGGACGC AGGTGGGCTC TGGGGAAGCA
    GTCACAGAGG AGTCACCAGC ACCCCCAAAT GAAGCTACTC CAACAGCAGC TCCTCCCACG
    TTGCCCCCGA CCACGGTGAG TGTGACGGGC GTTGTGAGCA GTACAGACGC TACTGCCACC
    GCTGCCACCA CCGAAGCCAC AACAGTCCCC ACCATCCCAA GTGTCGCACC TACCACCATG
    GCCACCACCA CCGTCGCCAC AACTACAACC ACTGCCGCCA CCACCATGGA GAGGCCTCCC
    ACCACCACCT CCAAGACTAA GATGCCCGAA TCCGCCCCCG ACAGGCAGTC CATATGGAAC
    GTCACGGTGC TCCCCAACAG TAAATGGGCC AACATCACCT GGAGACACAA TCTCGGGCCC
    GGAACTGCCT TTGTGGTTGA GTACATCGAC AGCAACCATA CGAAAAAAAC TGTCCCTGTT
    AAGAGCCAGG TCCAGCCCGT ACAGCTGACA GACCTCTATC CCGGGATGAC ATACACGTTG
    CGCGTTTATT CCCGGGACAA GGAGGGCATC AGCAGTACCG TCATCACCTT TATGACCAGT
    ACAGCTTACA CCAATAACCA GGCAGACATC GCCACCCAGG GCTGGTTCAT TGGGCTCATG
    TGTGCCATCG CCCTCCTGGT GCTGATCCTG CTCATCGTCT GCTTCATCAA GAGGAGTCGA
    GGTGGCAAAT ACCCAGTGCG AGAAAAGAAG GACGTTCCTC TTGGCCCCGA AGACCCCAAG
    GAAGAGGATG GCTCCTTTGA CTACAGTGAC GAGGACAACA AGCCCCTGCA GGGCAGCCAG
    ACGTCCCTCG ATGGTACCAT CAAGCAGCAA GAGAGCGACG ACAGCCTGGT GGACTATGGC
    GAGGGTGGCG AGGGGCAGTT CAATGAGGAT GGGTCCTTCA TCGGCCAGTA CACGGTCAAA
    AAGGACAAAG AGGAGACAGA GGGCAATGAA AGCTCAGAGG CCACATCGCC TGTCAACGCC
    ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45310
    Clone ID Related Accession (Same CDS sequence) XM_005640775.2 , XM_005640775.3
    Accession Version XM_005640775.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurofascin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005640775.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGGACGGCCA GACCGACCCC GGGACTTGGA GCTCACTGAC
    CTGGCTGAGA GGAGTGTGAG GCTGACCTGG ATCCCAGGGG ATGATAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CAGGCAGCGT CAACTCTGCC ATCCTCCAGT TGTCCCCATA TGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAACGAGGTG GGCAGCAGCC ATCCCAGCCT CCCCTCGGAG
    CGCTACAGGA CCAGCGGGGC GCCCCCTGAG TCCAATCCCG CCGACGTAAA GGGAGAAGGG
    ACCAGAAAGA ACAACATGGA GATCACTTGG ACGCCCATGA ACGCCACCTC TGCCTTTGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAAA CTCGAGAGAC GTGGAACAAC
    GCCACAGTGT GGGGCTCCCG CTACGTGGTG GGCCAGACCC CTGTCTACGT GCCCTATGAG
    ATCCGAGTCC AGGCCGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCCGGGG AAGACTATCC CAGGGCTGCA CCCACTGACG TTAAAATCCG AGTCCTGAAC
    AGCACAGCCA TCAGTCTCCA GTGGAACCGC GTTTACCCTG ACACAGTCCA GGGCCAGCTC
    AGAGAGTACC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGAC AGCAAGCCAG CTTCCCTGGT GACCGCCCCC GCGGCGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTCGTGGTCA ATGGGAGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGACGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC GGAACACCCC
    AATGGGATCC TGATCGGATA CACTCTCAAA TATGTGGCTT TTAATGGAAC CAAATTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGATGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TACCCTCAGT GCCAGGACGC AGGTGGGCTC TGGGGAAGCA
    GTCACAGAGG AGTCACCAGC ACCCCCAAAT GAAGCTACTC CAACAGCAGC TCCTCCCACG
    TTGCCCCCGA CCACGGTGAG TGTGACGGGC GTTGTGAGCA GTACAGACGC TACTGCCACC
    GCTGCCACCA CCGAAGCCAC AACAGTCCCC ACCATCCCAA GTGTCGCACC TACCACCATG
    GCCACCACCA CCGTCGCCAC AACTACAACC ACTGCCGCCA CCACCATGGA GAGGCCTCCC
    ACCACCACCT CCAAGACTAA GATGCCCGAA TCCGCCCCCG ACAGGCAGTC CATATGGAAC
    GTCACGGTGC TCCCCAACAG TAAATGGGCC AACATCACCT GGAGACACAA TCTCGGGCCC
    GGAACTGCCT TTGTGGTTGA GTACATCGAC AGCAACCATA CGAAAAAAAC TGTCCCTGTT
    AAGAGCCAGG TCCAGCCCGT ACAGCTGACA GACCTCTATC CCGGGATGAC ATACACGTTG
    CGCGTTTATT CCCGGGACAA GGAGGGCATC AGCAGTACCG TCATCACCTT TATGACCAGT
    ACAGCTTACA CCAATAACCA GGCAGACATC GCCACCCAGG GCTGGTTCAT TGGGCTCATG
    TGTGCCATCG CCCTCCTGGT GCTGATCCTG CTCATCGTCT GCTTCATCAA GAGGAGTCGA
    GGTGGCAAAT ACCCAGTGCG AGAAAAGAAG GACGTTCCTC TTGGCCCCGA AGACCCCAAG
    GAAGAGGATG GCTCCTTTGA CTACAGTGAC GAGGACAACA AGCCCCTGCA GGGCAGCCAG
    ACGTCCCTCG ATGGTACCAT CAAGCAGCAA GAGAGCGACG ACAGCCTGGT GGACTATGGC
    GAGGGTGGCG AGGGGCAGTT CAATGAGGAT GGGTCCTTCA TCGGCCAGTA CACGGTCAAA
    AAGGACAAAG AGGAGACAGA GGGCAATGAA AGCTCAGAGG CCACATCGCC TGTCAACGCC
    ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640832.2
    CDS304..4341
    Translation

    Target ORF information:

    RefSeq Version XM_005640775.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640775.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGGACGGCCA GACCGACCCC GGGACTTGGA GCTCACTGAC
    CTGGCTGAGA GGAGTGTGAG GCTGACCTGG ATCCCAGGGG ATGATAACAA CAGCCCCATC
    ACAGACTACG TCGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CAGGCAGCGT CAACTCTGCC ATCCTCCAGT TGTCCCCATA TGTCAACTAC
    CAGTTCCGGG TCATCGCCAT CAACGAGGTG GGCAGCAGCC ATCCCAGCCT CCCCTCGGAG
    CGCTACAGGA CCAGCGGGGC GCCCCCTGAG TCCAATCCCG CCGACGTAAA GGGAGAAGGG
    ACCAGAAAGA ACAACATGGA GATCACTTGG ACGCCCATGA ACGCCACCTC TGCCTTTGGC
    CCCAACCTGC GCTACATCGT CAAGTGGCGG CGGAGAGAAA CTCGAGAGAC GTGGAACAAC
    GCCACAGTGT GGGGCTCCCG CTACGTGGTG GGCCAGACCC CTGTCTACGT GCCCTATGAG
    ATCCGAGTCC AGGCCGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC TGTCATCGGT
    TACTCCGGGG AAGACTATCC CAGGGCTGCA CCCACTGACG TTAAAATCCG AGTCCTGAAC
    AGCACAGCCA TCAGTCTCCA GTGGAACCGC GTTTACCCTG ACACAGTCCA GGGCCAGCTC
    AGAGAGTACC GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGAC AGCAAGCCAG CTTCCCTGGT GACCGCCCCC GCGGCGTGGT GTCCCGCCTC
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTCGTGGTCA ATGGGAGAGG CGATGGGCCT
    CGCAGTGAAA CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGACGTTTC
    CGAGTCCGGC AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC GGAACACCCC
    AATGGGATCC TGATCGGATA CACTCTCAAA TATGTGGCTT TTAATGGAAC CAAATTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGATGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TACCCTCAGT GCCAGGACGC AGGTGGGCTC TGGGGAAGCA
    GTCACAGAGG AGTCACCAGC ACCCCCAAAT GAAGCTACTC CAACAGCAGC TCCTCCCACG
    TTGCCCCCGA CCACGGTGAG TGTGACGGGC GTTGTGAGCA GTACAGACGC TACTGCCACC
    GCTGCCACCA CCGAAGCCAC AACAGTCCCC ACCATCCCAA GTGTCGCACC TACCACCATG
    GCCACCACCA CCGTCGCCAC AACTACAACC ACTGCCGCCA CCACCATGGA GAGGCCTCCC
    ACCACCACCT CCAAGACTAA GATGCCCGAA TCCGCCCCCG ACAGGCAGTC CATATGGAAC
    GTCACGGTGC TCCCCAACAG TAAATGGGCC AACATCACCT GGAGACACAA TCTCGGGCCC
    GGAACTGCCT TTGTGGTTGA GTACATCGAC AGCAACCATA CGAAAAAAAC TGTCCCTGTT
    AAGAGCCAGG TCCAGCCCGT ACAGCTGACA GACCTCTATC CCGGGATGAC ATACACGTTG
    CGCGTTTATT CCCGGGACAA GGAGGGCATC AGCAGTACCG TCATCACCTT TATGACCAGT
    ACAGCTTACA CCAATAACCA GGCAGACATC GCCACCCAGG GCTGGTTCAT TGGGCTCATG
    TGTGCCATCG CCCTCCTGGT GCTGATCCTG CTCATCGTCT GCTTCATCAA GAGGAGTCGA
    GGTGGCAAAT ACCCAGTGCG AGAAAAGAAG GACGTTCCTC TTGGCCCCGA AGACCCCAAG
    GAAGAGGATG GCTCCTTTGA CTACAGTGAC GAGGACAACA AGCCCCTGCA GGGCAGCCAG
    ACGTCCCTCG ATGGTACCAT CAAGCAGCAA GAGAGCGACG ACAGCCTGGT GGACTATGGC
    GAGGGTGGCG AGGGGCAGTT CAATGAGGAT GGGTCCTTCA TCGGCCAGTA CACGGTCAAA
    AAGGACAAAG AGGAGACAGA GGGCAATGAA AGCTCAGAGG CCACATCGCC TGTCAACGCC
    ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45311
    Clone ID Related Accession (Same CDS sequence) XM_005640776.3 , XM_005640776.2
    Accession Version XM_005640776.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4032bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurofascin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005640776.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCACACG AGGAGTTGCA
    GAAAGGACAC CCAGCTTCAT GTATCCCCAG GGCACAGCGA GCAGTCAGAT GGTGCTGCGT
    GGCACGGATC TTCTGCTGGA GTGCATCGCC TCTGGGGTCC CCACGCCAGA CATCGCATGG
    TACAAGAAAG GTGGGGACCT CCCATCTGAC AAGGCCAAGT TTGAGAACTT TAACAAGGCT
    CTGCGTATCA CCAACGTCTC CGAGGAAGAC TCTGGGGAGT ATTTCTGCCT GGCCTCCAAC
    AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG
    GACGAACCCA AGAACCTTAT CCTGGCTCCC GGTGAGGACG GGAGACTGGT GTGTCGAGCC
    AATGGGAACC CCAAGCCCAC TGTCCAGTGG ATGGTAAATG GGGAACCACT GCAATCGGCA
    CCACCCAACC CAAACCGTGA GGTGGCTGGG GACACCATCA TCTTCCGGGA CACCCAGATC
    AGCAGCAGAG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT ACTGGCCAAC
    GCCTTCGTCA GCGTGCTGGA CGTGCCACCC CGGATGCTGT CACCCCGGAA CCAGCTCATT
    AGGGTGATCC TGTACAACCG GACACGGCTG GACTGCCCAT TCTTCGGGTC TCCCATCCCC
    ACACTCCGAT GGTTTAAGAA CGGGCAGGGA AGTAACCTGG ATGGTGGTAA CTACCACGTC
    TACGAGAATG GCAGTCTGGA AATCAAGATG ATCCGCAAAG AGGACCAAGG CATCTACACC
    TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAGAACCAGG TCCGCCTGGA GGTCAAAGAC
    CCCACCAGGA TCTACCGGAT GCCTGAAGAC CAGGTGGCCA AAAGGGGCAC GACGGTGCAG
    CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAGCTCA CGGTCTCCTG GCTGAAGGAT
    GATGAGCCTC TCTACATAGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT
    GGTGTGGCTG AGCGGGACCA GGGCAGTTAC ACATGTGTTG CCAGCACTGA GCTGGACCAG
    GACCTGGCCA AGGCCTACCT CACCGTGCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG
    GCTGCCCTGC CCAAAGGACG GCCAGACCGA CCCCGGGACT TGGAGCTCAC TGACCTGGCT
    GAGAGGAGTG TGAGGCTGAC CTGGATCCCA GGGGATGATA ACAACAGCCC CATCACAGAC
    TACGTCGTCC AGTTTGAAGA GGACCAGTTC CAGCCAGGGG TCTGGCATGA CCATTCCAAG
    TTCCCAGGCA GCGTCAACTC TGCCATCCTC CAGTTGTCCC CATATGTCAA CTACCAGTTC
    CGGGTCATCG CCATCAACGA GGTGGGCAGC AGCCATCCCA GCCTCCCCTC GGAGCGCTAC
    AGGACCAGCG GGGCGCCCCC TGAGTCCAAT CCCGCCGACG TAAAGGGAGA AGGGACCAGA
    AAGAACAACA TGGAGATCAC TTGGACGCCC ATGAACGCCA CCTCTGCCTT TGGCCCCAAC
    CTGCGCTACA TCGTCAAGTG GCGGCGGAGA GAAACTCGAG AGACGTGGAA CAACGCCACA
    GTGTGGGGCT CCCGCTACGT GGTGGGCCAG ACCCCTGTCT ACGTGCCCTA TGAGATCCGA
    GTCCAGGCCG AAAATGACTT TGGGAAGGGC CCTGAGCCAG ACACTGTCAT CGGTTACTCC
    GGGGAAGACT ATCCCAGGGC TGCACCCACT GACGTTAAAA TCCGAGTCCT GAACAGCACA
    GCCATCAGTC TCCAGTGGAA CCGCGTTTAC CCTGACACAG TCCAGGGCCA GCTCAGAGAG
    TACCGAGCCT ACTACTGGAG GGAGAGCAGC TTGCTGAAGA ACCTGTGGGT GTCTCAGAAG
    AGACAGCAAG CCAGCTTCCC TGGTGACCGC CCCCGCGGCG TGGTGTCCCG CCTCTTCCCC
    TACAGTAACT ACAAGCTGGA GATGGTCGTG GTCAATGGGA GAGGCGATGG GCCTCGCAGT
    GAAACCAAGG AGTTCACCAC CCCGGAAGGA GTACCCAGTG CCCCCAGACG TTTCCGAGTC
    CGGCAGCCCA ACCTGGAGAC AATCAACCTG GAATGGGATC ATCCGGAACA CCCCAATGGG
    ATCCTGATCG GATACACTCT CAAATATGTG GCTTTTAATG GAACCAAATT AGGAAAGCAG
    ATAGTGGAAA ACTTCTCTCC CAATCAGACC AAGTTCACGA TGCAAAGAGC AGACCCCGTG
    TCGCGCTACC GCTTTACCCT CAGTGCCAGG ACGCAGGTGG GCTCTGGGGA AGCAGTCACA
    GAGGAGTCAC CAGCACCCCC AAATGAAGCT ACTCCAACAG CAGCTCCTCC CACGTTGCCC
    CCGACCACGG TGAGTGTGAC GGGCGTTGTG AGCAGTACAG ACGCTACTGC CACCGCTGCC
    ACCACCGAAG CCACAACAGT CCCCACCATC CCAAGTGTCG CACCTACCAC CATGGCCACC
    ACCACCGTCG CCACAACTAC AACCACTGCC GCCACCACCA TGGAGAGGCC TCCCACCACC
    ACCTCCAAGA CTAAGATGCC CGAATCCGCC CCCGACAGGC AGTCCATATG GAACGTCACG
    GTGCTCCCCA ACAGTAAATG GGCCAACATC ACCTGGAGAC ACAATCTCGG GCCCGGAACT
    GCCTTTGTGG TTGAGTACAT CGACAGCAAC CATACGAAAA AAACTGTCCC TGTTAAGAGC
    CAGGTCCAGC CCGTACAGCT GACAGACCTC TATCCCGGGA TGACATACAC GTTGCGCGTT
    TATTCCCGGG ACAAGGAGGG CATCAGCAGT ACCGTCATCA CCTTTATGAC CAGTACAGCT
    TACACCAATA ACCAGGCAGA CATCGCCACC CAGGGCTGGT TCATTGGGCT CATGTGTGCC
    ATCGCCCTCC TGGTGCTGAT CCTGCTCATC GTCTGCTTCA TCAAGAGGAG TCGAGGTGGC
    AAATACCCAG TGCGAGAAAA GAAGGACGTT CCTCTTGGCC CCGAAGACCC CAAGGAAGAG
    GATGGCTCCT TTGACTACAG TGACGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACGTCC
    CTCGATGGTA CCATCAAGCA GCAAGAGAGC GACGACAGCC TGGTGGACTA TGGCGAGGGT
    GGCGAGGGGC AGTTCAATGA GGATGGGTCC TTCATCGGCC AGTACACGGT CAAAAAGGAC
    AAAGAGGAGA CAGAGGGCAA TGAAAGCTCA GAGGCCACAT CGCCTGTCAA CGCCATCTAT
    TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640833.2
    CDS304..4335
    Translation

    Target ORF information:

    RefSeq Version XM_005640776.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640776.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCACACG AGGAGTTGCA
    GAAAGGACAC CCAGCTTCAT GTATCCCCAG GGCACAGCGA GCAGTCAGAT GGTGCTGCGT
    GGCACGGATC TTCTGCTGGA GTGCATCGCC TCTGGGGTCC CCACGCCAGA CATCGCATGG
    TACAAGAAAG GTGGGGACCT CCCATCTGAC AAGGCCAAGT TTGAGAACTT TAACAAGGCT
    CTGCGTATCA CCAACGTCTC CGAGGAAGAC TCTGGGGAGT ATTTCTGCCT GGCCTCCAAC
    AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG
    GACGAACCCA AGAACCTTAT CCTGGCTCCC GGTGAGGACG GGAGACTGGT GTGTCGAGCC
    AATGGGAACC CCAAGCCCAC TGTCCAGTGG ATGGTAAATG GGGAACCACT GCAATCGGCA
    CCACCCAACC CAAACCGTGA GGTGGCTGGG GACACCATCA TCTTCCGGGA CACCCAGATC
    AGCAGCAGAG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT ACTGGCCAAC
    GCCTTCGTCA GCGTGCTGGA CGTGCCACCC CGGATGCTGT CACCCCGGAA CCAGCTCATT
    AGGGTGATCC TGTACAACCG GACACGGCTG GACTGCCCAT TCTTCGGGTC TCCCATCCCC
    ACACTCCGAT GGTTTAAGAA CGGGCAGGGA AGTAACCTGG ATGGTGGTAA CTACCACGTC
    TACGAGAATG GCAGTCTGGA AATCAAGATG ATCCGCAAAG AGGACCAAGG CATCTACACC
    TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAGAACCAGG TCCGCCTGGA GGTCAAAGAC
    CCCACCAGGA TCTACCGGAT GCCTGAAGAC CAGGTGGCCA AAAGGGGCAC GACGGTGCAG
    CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAGCTCA CGGTCTCCTG GCTGAAGGAT
    GATGAGCCTC TCTACATAGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT
    GGTGTGGCTG AGCGGGACCA GGGCAGTTAC ACATGTGTTG CCAGCACTGA GCTGGACCAG
    GACCTGGCCA AGGCCTACCT CACCGTGCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG
    GCTGCCCTGC CCAAAGGACG GCCAGACCGA CCCCGGGACT TGGAGCTCAC TGACCTGGCT
    GAGAGGAGTG TGAGGCTGAC CTGGATCCCA GGGGATGATA ACAACAGCCC CATCACAGAC
    TACGTCGTCC AGTTTGAAGA GGACCAGTTC CAGCCAGGGG TCTGGCATGA CCATTCCAAG
    TTCCCAGGCA GCGTCAACTC TGCCATCCTC CAGTTGTCCC CATATGTCAA CTACCAGTTC
    CGGGTCATCG CCATCAACGA GGTGGGCAGC AGCCATCCCA GCCTCCCCTC GGAGCGCTAC
    AGGACCAGCG GGGCGCCCCC TGAGTCCAAT CCCGCCGACG TAAAGGGAGA AGGGACCAGA
    AAGAACAACA TGGAGATCAC TTGGACGCCC ATGAACGCCA CCTCTGCCTT TGGCCCCAAC
    CTGCGCTACA TCGTCAAGTG GCGGCGGAGA GAAACTCGAG AGACGTGGAA CAACGCCACA
    GTGTGGGGCT CCCGCTACGT GGTGGGCCAG ACCCCTGTCT ACGTGCCCTA TGAGATCCGA
    GTCCAGGCCG AAAATGACTT TGGGAAGGGC CCTGAGCCAG ACACTGTCAT CGGTTACTCC
    GGGGAAGACT ATCCCAGGGC TGCACCCACT GACGTTAAAA TCCGAGTCCT GAACAGCACA
    GCCATCAGTC TCCAGTGGAA CCGCGTTTAC CCTGACACAG TCCAGGGCCA GCTCAGAGAG
    TACCGAGCCT ACTACTGGAG GGAGAGCAGC TTGCTGAAGA ACCTGTGGGT GTCTCAGAAG
    AGACAGCAAG CCAGCTTCCC TGGTGACCGC CCCCGCGGCG TGGTGTCCCG CCTCTTCCCC
    TACAGTAACT ACAAGCTGGA GATGGTCGTG GTCAATGGGA GAGGCGATGG GCCTCGCAGT
    GAAACCAAGG AGTTCACCAC CCCGGAAGGA GTACCCAGTG CCCCCAGACG TTTCCGAGTC
    CGGCAGCCCA ACCTGGAGAC AATCAACCTG GAATGGGATC ATCCGGAACA CCCCAATGGG
    ATCCTGATCG GATACACTCT CAAATATGTG GCTTTTAATG GAACCAAATT AGGAAAGCAG
    ATAGTGGAAA ACTTCTCTCC CAATCAGACC AAGTTCACGA TGCAAAGAGC AGACCCCGTG
    TCGCGCTACC GCTTTACCCT CAGTGCCAGG ACGCAGGTGG GCTCTGGGGA AGCAGTCACA
    GAGGAGTCAC CAGCACCCCC AAATGAAGCT ACTCCAACAG CAGCTCCTCC CACGTTGCCC
    CCGACCACGG TGAGTGTGAC GGGCGTTGTG AGCAGTACAG ACGCTACTGC CACCGCTGCC
    ACCACCGAAG CCACAACAGT CCCCACCATC CCAAGTGTCG CACCTACCAC CATGGCCACC
    ACCACCGTCG CCACAACTAC AACCACTGCC GCCACCACCA TGGAGAGGCC TCCCACCACC
    ACCTCCAAGA CTAAGATGCC CGAATCCGCC CCCGACAGGC AGTCCATATG GAACGTCACG
    GTGCTCCCCA ACAGTAAATG GGCCAACATC ACCTGGAGAC ACAATCTCGG GCCCGGAACT
    GCCTTTGTGG TTGAGTACAT CGACAGCAAC CATACGAAAA AAACTGTCCC TGTTAAGAGC
    CAGGTCCAGC CCGTACAGCT GACAGACCTC TATCCCGGGA TGACATACAC GTTGCGCGTT
    TATTCCCGGG ACAAGGAGGG CATCAGCAGT ACCGTCATCA CCTTTATGAC CAGTACAGCT
    TACACCAATA ACCAGGCAGA CATCGCCACC CAGGGCTGGT TCATTGGGCT CATGTGTGCC
    ATCGCCCTCC TGGTGCTGAT CCTGCTCATC GTCTGCTTCA TCAAGAGGAG TCGAGGTGGC
    AAATACCCAG TGCGAGAAAA GAAGGACGTT CCTCTTGGCC CCGAAGACCC CAAGGAAGAG
    GATGGCTCCT TTGACTACAG TGACGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACGTCC
    CTCGATGGTA CCATCAAGCA GCAAGAGAGC GACGACAGCC TGGTGGACTA TGGCGAGGGT
    GGCGAGGGGC AGTTCAATGA GGATGGGTCC TTCATCGGCC AGTACACGGT CAAAAAGGAC
    AAAGAGGAGA CAGAGGGCAA TGAAAGCTCA GAGGCCACAT CGCCTGTCAA CGCCATCTAT
    TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45311
    Clone ID Related Accession (Same CDS sequence) XM_005640776.3 , XM_005640776.2
    Accession Version XM_005640776.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4032bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neurofascin isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640776.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCACACG AGGAGTTGCA
    GAAAGGACAC CCAGCTTCAT GTATCCCCAG GGCACAGCGA GCAGTCAGAT GGTGCTGCGT
    GGCACGGATC TTCTGCTGGA GTGCATCGCC TCTGGGGTCC CCACGCCAGA CATCGCATGG
    TACAAGAAAG GTGGGGACCT CCCATCTGAC AAGGCCAAGT TTGAGAACTT TAACAAGGCT
    CTGCGTATCA CCAACGTCTC CGAGGAAGAC TCTGGGGAGT ATTTCTGCCT GGCCTCCAAC
    AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG
    GACGAACCCA AGAACCTTAT CCTGGCTCCC GGTGAGGACG GGAGACTGGT GTGTCGAGCC
    AATGGGAACC CCAAGCCCAC TGTCCAGTGG ATGGTAAATG GGGAACCACT GCAATCGGCA
    CCACCCAACC CAAACCGTGA GGTGGCTGGG GACACCATCA TCTTCCGGGA CACCCAGATC
    AGCAGCAGAG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT ACTGGCCAAC
    GCCTTCGTCA GCGTGCTGGA CGTGCCACCC CGGATGCTGT CACCCCGGAA CCAGCTCATT
    AGGGTGATCC TGTACAACCG GACACGGCTG GACTGCCCAT TCTTCGGGTC TCCCATCCCC
    ACACTCCGAT GGTTTAAGAA CGGGCAGGGA AGTAACCTGG ATGGTGGTAA CTACCACGTC
    TACGAGAATG GCAGTCTGGA AATCAAGATG ATCCGCAAAG AGGACCAAGG CATCTACACC
    TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAGAACCAGG TCCGCCTGGA GGTCAAAGAC
    CCCACCAGGA TCTACCGGAT GCCTGAAGAC CAGGTGGCCA AAAGGGGCAC GACGGTGCAG
    CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAGCTCA CGGTCTCCTG GCTGAAGGAT
    GATGAGCCTC TCTACATAGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT
    GGTGTGGCTG AGCGGGACCA GGGCAGTTAC ACATGTGTTG CCAGCACTGA GCTGGACCAG
    GACCTGGCCA AGGCCTACCT CACCGTGCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG
    GCTGCCCTGC CCAAAGGACG GCCAGACCGA CCCCGGGACT TGGAGCTCAC TGACCTGGCT
    GAGAGGAGTG TGAGGCTGAC CTGGATCCCA GGGGATGATA ACAACAGCCC CATCACAGAC
    TACGTCGTCC AGTTTGAAGA GGACCAGTTC CAGCCAGGGG TCTGGCATGA CCATTCCAAG
    TTCCCAGGCA GCGTCAACTC TGCCATCCTC CAGTTGTCCC CATATGTCAA CTACCAGTTC
    CGGGTCATCG CCATCAACGA GGTGGGCAGC AGCCATCCCA GCCTCCCCTC GGAGCGCTAC
    AGGACCAGCG GGGCGCCCCC TGAGTCCAAT CCCGCCGACG TAAAGGGAGA AGGGACCAGA
    AAGAACAACA TGGAGATCAC TTGGACGCCC ATGAACGCCA CCTCTGCCTT TGGCCCCAAC
    CTGCGCTACA TCGTCAAGTG GCGGCGGAGA GAAACTCGAG AGACGTGGAA CAACGCCACA
    GTGTGGGGCT CCCGCTACGT GGTGGGCCAG ACCCCTGTCT ACGTGCCCTA TGAGATCCGA
    GTCCAGGCCG AAAATGACTT TGGGAAGGGC CCTGAGCCAG ACACTGTCAT CGGTTACTCC
    GGGGAAGACT ATCCCAGGGC TGCACCCACT GACGTTAAAA TCCGAGTCCT GAACAGCACA
    GCCATCAGTC TCCAGTGGAA CCGCGTTTAC CCTGACACAG TCCAGGGCCA GCTCAGAGAG
    TACCGAGCCT ACTACTGGAG GGAGAGCAGC TTGCTGAAGA ACCTGTGGGT GTCTCAGAAG
    AGACAGCAAG CCAGCTTCCC TGGTGACCGC CCCCGCGGCG TGGTGTCCCG CCTCTTCCCC
    TACAGTAACT ACAAGCTGGA GATGGTCGTG GTCAATGGGA GAGGCGATGG GCCTCGCAGT
    GAAACCAAGG AGTTCACCAC CCCGGAAGGA GTACCCAGTG CCCCCAGACG TTTCCGAGTC
    CGGCAGCCCA ACCTGGAGAC AATCAACCTG GAATGGGATC ATCCGGAACA CCCCAATGGG
    ATCCTGATCG GATACACTCT CAAATATGTG GCTTTTAATG GAACCAAATT AGGAAAGCAG
    ATAGTGGAAA ACTTCTCTCC CAATCAGACC AAGTTCACGA TGCAAAGAGC AGACCCCGTG
    TCGCGCTACC GCTTTACCCT CAGTGCCAGG ACGCAGGTGG GCTCTGGGGA AGCAGTCACA
    GAGGAGTCAC CAGCACCCCC AAATGAAGCT ACTCCAACAG CAGCTCCTCC CACGTTGCCC
    CCGACCACGG TGAGTGTGAC GGGCGTTGTG AGCAGTACAG ACGCTACTGC CACCGCTGCC
    ACCACCGAAG CCACAACAGT CCCCACCATC CCAAGTGTCG CACCTACCAC CATGGCCACC
    ACCACCGTCG CCACAACTAC AACCACTGCC GCCACCACCA TGGAGAGGCC TCCCACCACC
    ACCTCCAAGA CTAAGATGCC CGAATCCGCC CCCGACAGGC AGTCCATATG GAACGTCACG
    GTGCTCCCCA ACAGTAAATG GGCCAACATC ACCTGGAGAC ACAATCTCGG GCCCGGAACT
    GCCTTTGTGG TTGAGTACAT CGACAGCAAC CATACGAAAA AAACTGTCCC TGTTAAGAGC
    CAGGTCCAGC CCGTACAGCT GACAGACCTC TATCCCGGGA TGACATACAC GTTGCGCGTT
    TATTCCCGGG ACAAGGAGGG CATCAGCAGT ACCGTCATCA CCTTTATGAC CAGTACAGCT
    TACACCAATA ACCAGGCAGA CATCGCCACC CAGGGCTGGT TCATTGGGCT CATGTGTGCC
    ATCGCCCTCC TGGTGCTGAT CCTGCTCATC GTCTGCTTCA TCAAGAGGAG TCGAGGTGGC
    AAATACCCAG TGCGAGAAAA GAAGGACGTT CCTCTTGGCC CCGAAGACCC CAAGGAAGAG
    GATGGCTCCT TTGACTACAG TGACGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACGTCC
    CTCGATGGTA CCATCAAGCA GCAAGAGAGC GACGACAGCC TGGTGGACTA TGGCGAGGGT
    GGCGAGGGGC AGTTCAATGA GGATGGGTCC TTCATCGGCC AGTACACGGT CAAAAAGGAC
    AAAGAGGAGA CAGAGGGCAA TGAAAGCTCA GAGGCCACAT CGCCTGTCAA CGCCATCTAT
    TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640833.2
    CDS288..4319
    Translation

    Target ORF information:

    RefSeq Version XM_005640776.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640776.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCACACG AGGAGTTGCA
    GAAAGGACAC CCAGCTTCAT GTATCCCCAG GGCACAGCGA GCAGTCAGAT GGTGCTGCGT
    GGCACGGATC TTCTGCTGGA GTGCATCGCC TCTGGGGTCC CCACGCCAGA CATCGCATGG
    TACAAGAAAG GTGGGGACCT CCCATCTGAC AAGGCCAAGT TTGAGAACTT TAACAAGGCT
    CTGCGTATCA CCAACGTCTC CGAGGAAGAC TCTGGGGAGT ATTTCTGCCT GGCCTCCAAC
    AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG
    GACGAACCCA AGAACCTTAT CCTGGCTCCC GGTGAGGACG GGAGACTGGT GTGTCGAGCC
    AATGGGAACC CCAAGCCCAC TGTCCAGTGG ATGGTAAATG GGGAACCACT GCAATCGGCA
    CCACCCAACC CAAACCGTGA GGTGGCTGGG GACACCATCA TCTTCCGGGA CACCCAGATC
    AGCAGCAGAG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT ACTGGCCAAC
    GCCTTCGTCA GCGTGCTGGA CGTGCCACCC CGGATGCTGT CACCCCGGAA CCAGCTCATT
    AGGGTGATCC TGTACAACCG GACACGGCTG GACTGCCCAT TCTTCGGGTC TCCCATCCCC
    ACACTCCGAT GGTTTAAGAA CGGGCAGGGA AGTAACCTGG ATGGTGGTAA CTACCACGTC
    TACGAGAATG GCAGTCTGGA AATCAAGATG ATCCGCAAAG AGGACCAAGG CATCTACACC
    TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAGAACCAGG TCCGCCTGGA GGTCAAAGAC
    CCCACCAGGA TCTACCGGAT GCCTGAAGAC CAGGTGGCCA AAAGGGGCAC GACGGTGCAG
    CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAGCTCA CGGTCTCCTG GCTGAAGGAT
    GATGAGCCTC TCTACATAGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT
    GGTGTGGCTG AGCGGGACCA GGGCAGTTAC ACATGTGTTG CCAGCACTGA GCTGGACCAG
    GACCTGGCCA AGGCCTACCT CACCGTGCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG
    GCTGCCCTGC CCAAAGGACG GCCAGACCGA CCCCGGGACT TGGAGCTCAC TGACCTGGCT
    GAGAGGAGTG TGAGGCTGAC CTGGATCCCA GGGGATGATA ACAACAGCCC CATCACAGAC
    TACGTCGTCC AGTTTGAAGA GGACCAGTTC CAGCCAGGGG TCTGGCATGA CCATTCCAAG
    TTCCCAGGCA GCGTCAACTC TGCCATCCTC CAGTTGTCCC CATATGTCAA CTACCAGTTC
    CGGGTCATCG CCATCAACGA GGTGGGCAGC AGCCATCCCA GCCTCCCCTC GGAGCGCTAC
    AGGACCAGCG GGGCGCCCCC TGAGTCCAAT CCCGCCGACG TAAAGGGAGA AGGGACCAGA
    AAGAACAACA TGGAGATCAC TTGGACGCCC ATGAACGCCA CCTCTGCCTT TGGCCCCAAC
    CTGCGCTACA TCGTCAAGTG GCGGCGGAGA GAAACTCGAG AGACGTGGAA CAACGCCACA
    GTGTGGGGCT CCCGCTACGT GGTGGGCCAG ACCCCTGTCT ACGTGCCCTA TGAGATCCGA
    GTCCAGGCCG AAAATGACTT TGGGAAGGGC CCTGAGCCAG ACACTGTCAT CGGTTACTCC
    GGGGAAGACT ATCCCAGGGC TGCACCCACT GACGTTAAAA TCCGAGTCCT GAACAGCACA
    GCCATCAGTC TCCAGTGGAA CCGCGTTTAC CCTGACACAG TCCAGGGCCA GCTCAGAGAG
    TACCGAGCCT ACTACTGGAG GGAGAGCAGC TTGCTGAAGA ACCTGTGGGT GTCTCAGAAG
    AGACAGCAAG CCAGCTTCCC TGGTGACCGC CCCCGCGGCG TGGTGTCCCG CCTCTTCCCC
    TACAGTAACT ACAAGCTGGA GATGGTCGTG GTCAATGGGA GAGGCGATGG GCCTCGCAGT
    GAAACCAAGG AGTTCACCAC CCCGGAAGGA GTACCCAGTG CCCCCAGACG TTTCCGAGTC
    CGGCAGCCCA ACCTGGAGAC AATCAACCTG GAATGGGATC ATCCGGAACA CCCCAATGGG
    ATCCTGATCG GATACACTCT CAAATATGTG GCTTTTAATG GAACCAAATT AGGAAAGCAG
    ATAGTGGAAA ACTTCTCTCC CAATCAGACC AAGTTCACGA TGCAAAGAGC AGACCCCGTG
    TCGCGCTACC GCTTTACCCT CAGTGCCAGG ACGCAGGTGG GCTCTGGGGA AGCAGTCACA
    GAGGAGTCAC CAGCACCCCC AAATGAAGCT ACTCCAACAG CAGCTCCTCC CACGTTGCCC
    CCGACCACGG TGAGTGTGAC GGGCGTTGTG AGCAGTACAG ACGCTACTGC CACCGCTGCC
    ACCACCGAAG CCACAACAGT CCCCACCATC CCAAGTGTCG CACCTACCAC CATGGCCACC
    ACCACCGTCG CCACAACTAC AACCACTGCC GCCACCACCA TGGAGAGGCC TCCCACCACC
    ACCTCCAAGA CTAAGATGCC CGAATCCGCC CCCGACAGGC AGTCCATATG GAACGTCACG
    GTGCTCCCCA ACAGTAAATG GGCCAACATC ACCTGGAGAC ACAATCTCGG GCCCGGAACT
    GCCTTTGTGG TTGAGTACAT CGACAGCAAC CATACGAAAA AAACTGTCCC TGTTAAGAGC
    CAGGTCCAGC CCGTACAGCT GACAGACCTC TATCCCGGGA TGACATACAC GTTGCGCGTT
    TATTCCCGGG ACAAGGAGGG CATCAGCAGT ACCGTCATCA CCTTTATGAC CAGTACAGCT
    TACACCAATA ACCAGGCAGA CATCGCCACC CAGGGCTGGT TCATTGGGCT CATGTGTGCC
    ATCGCCCTCC TGGTGCTGAT CCTGCTCATC GTCTGCTTCA TCAAGAGGAG TCGAGGTGGC
    AAATACCCAG TGCGAGAAAA GAAGGACGTT CCTCTTGGCC CCGAAGACCC CAAGGAAGAG
    GATGGCTCCT TTGACTACAG TGACGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACGTCC
    CTCGATGGTA CCATCAAGCA GCAAGAGAGC GACGACAGCC TGGTGGACTA TGGCGAGGGT
    GGCGAGGGGC AGTTCAATGA GGATGGGTCC TTCATCGGCC AGTACACGGT CAAAAAGGAC
    AAAGAGGAGA CAGAGGGCAA TGAAAGCTCA GAGGCCACAT CGCCTGTCAA CGCCATCTAT
    TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45312
    Clone ID Related Accession (Same CDS sequence) XM_014111177.1 , XM_014111177.2
    Accession Version XM_014111177.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neurofascin isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CTATCCCAGC CCCCAACCAT CACCAAGCAG
    TCAGTGAAGG ACCACATCGT GGATCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAG
    GGGAACCCTG CCCCCAGCTT CCACTGGACT CGAAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCAATGAG GAGAAAGTCT GGGACCCTGG TGATCGACTT CCGTAGTGGT
    GGGCGGCCCG AAGAATATGA AGGGGAATAC CAGTGCTTTG CCCGCAACAA ATTTGGCACG
    GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAGTCTC CCCTGTGGCC CAAGGAGAAC
    CTAGACCCAG TCGTGGTTCA AGAAGGTGCT CCTTTGACAC TCCAGTGCAA CCCTCCACCC
    GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACTCAGGAC
    AAGCGTGTCT CTCAGGGCCA CAACGGTGAC CTGTATTTCT CCAACGTGAT GCTTCAGGAT
    ATGCAGACAG ACTACAGCTG CAACGCGCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCACA CGAGGAGTTG CAGAAAGGAC ACCCAGCTTC
    ATGTATCCCC AGGGCACAGC GAGCAGTCAG ATGGTGCTGC GTGGCACGGA TCTTCTGCTG
    GAGTGCATCG CCTCTGGGGT CCCCACGCCA GACATCGCAT GGTACAAGAA AGGTGGGGAC
    CTCCCATCTG ACAAGGCCAA GTTTGAGAAC TTTAACAAGG CTCTGCGTAT CACCAACGTC
    TCCGAGGAAG ACTCTGGGGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG
    CACACGATCT CGGTGAGAGT AAAGGCTGCT CCCTACTGGC TGGACGAACC CAAGAACCTT
    ATCCTGGCTC CCGGTGAGGA CGGGAGACTG GTGTGTCGAG CCAATGGGAA CCCCAAGCCC
    ACTGTCCAGT GGATGGTAAA TGGGGAACCA CTGCAATCGG CACCACCCAA CCCAAACCGT
    GAGGTGGCTG GGGACACCAT CATCTTCCGG GACACCCAGA TCAGCAGCAG AGCTGTGTAC
    CAGTGCAACA CCTCCAACGA GCATGGCTAC CTACTGGCCA ACGCCTTCGT CAGCGTGCTG
    GACGTGCCAC CCCGGATGCT GTCACCCCGG AACCAGCTCA TTAGGGTGAT CCTGTACAAC
    CGGACACGGC TGGACTGCCC ATTCTTCGGG TCTCCCATCC CCACACTCCG ATGGTTTAAG
    AACGGGCAGG GAAGTAACCT GGATGGTGGT AACTACCACG TCTACGAGAA TGGCAGTCTG
    GAAATCAAGA TGATCCGCAA AGAGGACCAA GGCATCTACA CCTGTGTCGC CACCAACATC
    CTGGGCAAAG CTGAGAACCA GGTCCGCCTG GAGGTCAAAG ACCCCACCAG GATCTACCGG
    ATGCCTGAAG ACCAGGTGGC CAAAAGGGGC ACGACGGTGC AGCTGGAGTG TCGGGTGAAG
    CACGACCCCT CCCTGAAGCT CACGGTCTCC TGGCTGAAGG ATGATGAGCC TCTCTACATA
    GGAAACAGGA TGAAGAAGGA AGACGACTCC CTGACCATCT TTGGTGTGGC TGAGCGGGAC
    CAGGGCAGTT ACACATGTGT TGCCAGCACT GAGCTGGACC AGGACCTGGC CAAGGCCTAC
    CTCACCGTGC TAGCTGATCA GGCCACTCCA ACTAACCGTT TGGCTGCCCT GCCCAAAGGA
    CGGCCAGACC GACCCCGGGA CTTGGAGCTC ACTGACCTGG CTGAGAGGAG TGTGAGGCTG
    ACCTGGATCC CAGGGGATGA TAACAACAGC CCCATCACAG ACTACGTCGT CCAGTTTGAA
    GAGGACCAGT TCCAGCCAGG GGTCTGGCAT GACCATTCCA AGTTCCCAGG CAGCGTCAAC
    TCTGCCATCC TCCAGTTGTC CCCATATGTC AACTACCAGT TCCGGGTCAT CGCCATCAAC
    GAGGTGGGCA GCAGCCATCC CAGCCTCCCC TCGGAGCGCT ACAGGACCAG CGGGGCGCCC
    CCTGAGTCCA ATCCCGCCGA CGTAAAGGGA GAAGGGACCA GAAAGAACAA CATGGAGATC
    ACTTGGACGC CCATGAACGC CACCTCTGCC TTTGGCCCCA ACCTGCGCTA CATCGTCAAG
    TGGCGGCGGA GAGAAACTCG AGAGACGTGG AACAACGCCA CAGTGTGGGG CTCCCGCTAC
    GTGGTGGGCC AGACCCCTGT CTACGTGCCC TATGAGATCC GAGTCCAGGC CGAAAATGAC
    TTTGGGAAGG GCCCTGAGCC AGACACTGTC ATCGGTTACT CCGGGGAAGA CTATCCCAGG
    GCTGCACCCA CTGACGTTAA AATCCGAGTC CTGAACAGCA CAGCCATCAG TCTCCAGTGG
    AACCGCGTTT ACCCTGACAC AGTCCAGGGC CAGCTCAGAG AGTACCGAGC CTACTACTGG
    AGGGAGAGCA GCTTGCTGAA GAACCTGTGG GTGTCTCAGA AGAGACAGCA AGCCAGCTTC
    CCTGGTGACC GCCCCCGCGG CGTGGTGTCC CGCCTCTTCC CCTACAGTAA CTACAAGCTG
    GAGATGGTCG TGGTCAATGG GAGAGGCGAT GGGCCTCGCA GTGAAACCAA GGAGTTCACC
    ACCCCGGAAG GAGTACCCAG TGCCCCCAGA CGTTTCCGAG TCCGGCAGCC CAACCTGGAG
    ACAATCAACC TGGAATGGGA TCATCCGGAA CACCCCAATG GGATCCTGAT CGGATACACT
    CTCAAATATG TGGCTTTTAA TGGAACCAAA TTAGGAAAGC AGATAGTGGA AAACTTCTCT
    CCCAATCAGA CCAAGTTCAC GATGCAAAGA GCAGACCCCG TGTCGCGCTA CCGCTTTACC
    CTCAGTGCCA GGACGCAGGT GGGCTCTGGG GAAGCAGTCA CAGAGGAGTC ACCAGCACCC
    CCAAATGAAG CTACTCCAAC AGCAGCTCCT CCCACGTTGC CCCCGACCAC GGTGAGTGTG
    ACGGGCGTTG TGAGCAGTAC AGACGCTACT GCCACCGCTG CCACCACCGA AGCCACAACA
    GTCCCCACCA TCCCAAGTGT CGCACCTACC ACCATGGCCA CCACCACCGT CGCCACAACT
    ACAACCACTG CCGCCACCAC CATGGAGAGG CCTCCCACCA CCACCTCCAA GACTAAGATG
    CCCGAATCCG CCCCCGACAG GCAGTCCATA TGGAACGTCA CGGTGCTCCC CAACAGTAAA
    TGGGCCAACA TCACCTGGAG ACACAATCTC GGGCCCGGAA CTGCCTTTGT GGTTGAGTAC
    ATCGACAGCA ACCATACGAA AAAAACTGTC CCTGTTAAGA GCCAGGTCCA GCCCGTACAG
    CTGACAGACC TCTATCCCGG GATGACATAC ACGTTGCGCG TTTATTCCCG GGACAAGGAG
    GGCATCAGCA GTACCGTCAT CACCTTTATG ACCAGTACAG CTTACACCAA TAACCAGGCA
    GACATCGCCA CCCAGGGCTG GTTCATTGGG CTCATGTGTG CCATCGCCCT CCTGGTGCTG
    ATCCTGCTCA TCGTCTGCTT CATCAAGAGG AGTCGAGGTG GCAAATACCC AGTGCGAGAA
    AAGAAGGACG TTCCTCTTGG CCCCGAAGAC CCCAAGGAAG AGGATGGCTC CTTTGACTAC
    AGTGACGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACGT CCCTCGATGG TACCATCAAG
    CAGCAAGAGA GCGACGACAG CCTGGTGGAC TATGGCGAGG GTGGCGAGGG GCAGTTCAAT
    GAGGATGGGT CCTTCATCGG CCAGTACACG GTCAAAAAGG ACAAAGAGGA GACAGAGGGC
    AATGAAAGCT CAGAGGCCAC ATCGCCTGTC AACGCCATCT ATTCTCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013966652.1
    CDS288..4301
    Translation

    Target ORF information:

    RefSeq Version XM_014111177.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111177.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CTATCCCAGC CCCCAACCAT CACCAAGCAG
    TCAGTGAAGG ACCACATCGT GGATCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAG
    GGGAACCCTG CCCCCAGCTT CCACTGGACT CGAAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCAATGAG GAGAAAGTCT GGGACCCTGG TGATCGACTT CCGTAGTGGT
    GGGCGGCCCG AAGAATATGA AGGGGAATAC CAGTGCTTTG CCCGCAACAA ATTTGGCACG
    GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAGTCTC CCCTGTGGCC CAAGGAGAAC
    CTAGACCCAG TCGTGGTTCA AGAAGGTGCT CCTTTGACAC TCCAGTGCAA CCCTCCACCC
    GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACTCAGGAC
    AAGCGTGTCT CTCAGGGCCA CAACGGTGAC CTGTATTTCT CCAACGTGAT GCTTCAGGAT
    ATGCAGACAG ACTACAGCTG CAACGCGCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCACA CGAGGAGTTG CAGAAAGGAC ACCCAGCTTC
    ATGTATCCCC AGGGCACAGC GAGCAGTCAG ATGGTGCTGC GTGGCACGGA TCTTCTGCTG
    GAGTGCATCG CCTCTGGGGT CCCCACGCCA GACATCGCAT GGTACAAGAA AGGTGGGGAC
    CTCCCATCTG ACAAGGCCAA GTTTGAGAAC TTTAACAAGG CTCTGCGTAT CACCAACGTC
    TCCGAGGAAG ACTCTGGGGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG
    CACACGATCT CGGTGAGAGT AAAGGCTGCT CCCTACTGGC TGGACGAACC CAAGAACCTT
    ATCCTGGCTC CCGGTGAGGA CGGGAGACTG GTGTGTCGAG CCAATGGGAA CCCCAAGCCC
    ACTGTCCAGT GGATGGTAAA TGGGGAACCA CTGCAATCGG CACCACCCAA CCCAAACCGT
    GAGGTGGCTG GGGACACCAT CATCTTCCGG GACACCCAGA TCAGCAGCAG AGCTGTGTAC
    CAGTGCAACA CCTCCAACGA GCATGGCTAC CTACTGGCCA ACGCCTTCGT CAGCGTGCTG
    GACGTGCCAC CCCGGATGCT GTCACCCCGG AACCAGCTCA TTAGGGTGAT CCTGTACAAC
    CGGACACGGC TGGACTGCCC ATTCTTCGGG TCTCCCATCC CCACACTCCG ATGGTTTAAG
    AACGGGCAGG GAAGTAACCT GGATGGTGGT AACTACCACG TCTACGAGAA TGGCAGTCTG
    GAAATCAAGA TGATCCGCAA AGAGGACCAA GGCATCTACA CCTGTGTCGC CACCAACATC
    CTGGGCAAAG CTGAGAACCA GGTCCGCCTG GAGGTCAAAG ACCCCACCAG GATCTACCGG
    ATGCCTGAAG ACCAGGTGGC CAAAAGGGGC ACGACGGTGC AGCTGGAGTG TCGGGTGAAG
    CACGACCCCT CCCTGAAGCT CACGGTCTCC TGGCTGAAGG ATGATGAGCC TCTCTACATA
    GGAAACAGGA TGAAGAAGGA AGACGACTCC CTGACCATCT TTGGTGTGGC TGAGCGGGAC
    CAGGGCAGTT ACACATGTGT TGCCAGCACT GAGCTGGACC AGGACCTGGC CAAGGCCTAC
    CTCACCGTGC TAGCTGATCA GGCCACTCCA ACTAACCGTT TGGCTGCCCT GCCCAAAGGA
    CGGCCAGACC GACCCCGGGA CTTGGAGCTC ACTGACCTGG CTGAGAGGAG TGTGAGGCTG
    ACCTGGATCC CAGGGGATGA TAACAACAGC CCCATCACAG ACTACGTCGT CCAGTTTGAA
    GAGGACCAGT TCCAGCCAGG GGTCTGGCAT GACCATTCCA AGTTCCCAGG CAGCGTCAAC
    TCTGCCATCC TCCAGTTGTC CCCATATGTC AACTACCAGT TCCGGGTCAT CGCCATCAAC
    GAGGTGGGCA GCAGCCATCC CAGCCTCCCC TCGGAGCGCT ACAGGACCAG CGGGGCGCCC
    CCTGAGTCCA ATCCCGCCGA CGTAAAGGGA GAAGGGACCA GAAAGAACAA CATGGAGATC
    ACTTGGACGC CCATGAACGC CACCTCTGCC TTTGGCCCCA ACCTGCGCTA CATCGTCAAG
    TGGCGGCGGA GAGAAACTCG AGAGACGTGG AACAACGCCA CAGTGTGGGG CTCCCGCTAC
    GTGGTGGGCC AGACCCCTGT CTACGTGCCC TATGAGATCC GAGTCCAGGC CGAAAATGAC
    TTTGGGAAGG GCCCTGAGCC AGACACTGTC ATCGGTTACT CCGGGGAAGA CTATCCCAGG
    GCTGCACCCA CTGACGTTAA AATCCGAGTC CTGAACAGCA CAGCCATCAG TCTCCAGTGG
    AACCGCGTTT ACCCTGACAC AGTCCAGGGC CAGCTCAGAG AGTACCGAGC CTACTACTGG
    AGGGAGAGCA GCTTGCTGAA GAACCTGTGG GTGTCTCAGA AGAGACAGCA AGCCAGCTTC
    CCTGGTGACC GCCCCCGCGG CGTGGTGTCC CGCCTCTTCC CCTACAGTAA CTACAAGCTG
    GAGATGGTCG TGGTCAATGG GAGAGGCGAT GGGCCTCGCA GTGAAACCAA GGAGTTCACC
    ACCCCGGAAG GAGTACCCAG TGCCCCCAGA CGTTTCCGAG TCCGGCAGCC CAACCTGGAG
    ACAATCAACC TGGAATGGGA TCATCCGGAA CACCCCAATG GGATCCTGAT CGGATACACT
    CTCAAATATG TGGCTTTTAA TGGAACCAAA TTAGGAAAGC AGATAGTGGA AAACTTCTCT
    CCCAATCAGA CCAAGTTCAC GATGCAAAGA GCAGACCCCG TGTCGCGCTA CCGCTTTACC
    CTCAGTGCCA GGACGCAGGT GGGCTCTGGG GAAGCAGTCA CAGAGGAGTC ACCAGCACCC
    CCAAATGAAG CTACTCCAAC AGCAGCTCCT CCCACGTTGC CCCCGACCAC GGTGAGTGTG
    ACGGGCGTTG TGAGCAGTAC AGACGCTACT GCCACCGCTG CCACCACCGA AGCCACAACA
    GTCCCCACCA TCCCAAGTGT CGCACCTACC ACCATGGCCA CCACCACCGT CGCCACAACT
    ACAACCACTG CCGCCACCAC CATGGAGAGG CCTCCCACCA CCACCTCCAA GACTAAGATG
    CCCGAATCCG CCCCCGACAG GCAGTCCATA TGGAACGTCA CGGTGCTCCC CAACAGTAAA
    TGGGCCAACA TCACCTGGAG ACACAATCTC GGGCCCGGAA CTGCCTTTGT GGTTGAGTAC
    ATCGACAGCA ACCATACGAA AAAAACTGTC CCTGTTAAGA GCCAGGTCCA GCCCGTACAG
    CTGACAGACC TCTATCCCGG GATGACATAC ACGTTGCGCG TTTATTCCCG GGACAAGGAG
    GGCATCAGCA GTACCGTCAT CACCTTTATG ACCAGTACAG CTTACACCAA TAACCAGGCA
    GACATCGCCA CCCAGGGCTG GTTCATTGGG CTCATGTGTG CCATCGCCCT CCTGGTGCTG
    ATCCTGCTCA TCGTCTGCTT CATCAAGAGG AGTCGAGGTG GCAAATACCC AGTGCGAGAA
    AAGAAGGACG TTCCTCTTGG CCCCGAAGAC CCCAAGGAAG AGGATGGCTC CTTTGACTAC
    AGTGACGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACGT CCCTCGATGG TACCATCAAG
    CAGCAAGAGA GCGACGACAG CCTGGTGGAC TATGGCGAGG GTGGCGAGGG GCAGTTCAAT
    GAGGATGGGT CCTTCATCGG CCAGTACACG GTCAAAAAGG ACAAAGAGGA GACAGAGGGC
    AATGAAAGCT CAGAGGCCAC ATCGCCTGTC AACGCCATCT ATTCTCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45312
    Clone ID Related Accession (Same CDS sequence) XM_014111177.1 , XM_014111177.2
    Accession Version XM_014111177.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurofascin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111177.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CTATCCCAGC CCCCAACCAT CACCAAGCAG
    TCAGTGAAGG ACCACATCGT GGATCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAG
    GGGAACCCTG CCCCCAGCTT CCACTGGACT CGAAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCAATGAG GAGAAAGTCT GGGACCCTGG TGATCGACTT CCGTAGTGGT
    GGGCGGCCCG AAGAATATGA AGGGGAATAC CAGTGCTTTG CCCGCAACAA ATTTGGCACG
    GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAGTCTC CCCTGTGGCC CAAGGAGAAC
    CTAGACCCAG TCGTGGTTCA AGAAGGTGCT CCTTTGACAC TCCAGTGCAA CCCTCCACCC
    GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACTCAGGAC
    AAGCGTGTCT CTCAGGGCCA CAACGGTGAC CTGTATTTCT CCAACGTGAT GCTTCAGGAT
    ATGCAGACAG ACTACAGCTG CAACGCGCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCACA CGAGGAGTTG CAGAAAGGAC ACCCAGCTTC
    ATGTATCCCC AGGGCACAGC GAGCAGTCAG ATGGTGCTGC GTGGCACGGA TCTTCTGCTG
    GAGTGCATCG CCTCTGGGGT CCCCACGCCA GACATCGCAT GGTACAAGAA AGGTGGGGAC
    CTCCCATCTG ACAAGGCCAA GTTTGAGAAC TTTAACAAGG CTCTGCGTAT CACCAACGTC
    TCCGAGGAAG ACTCTGGGGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG
    CACACGATCT CGGTGAGAGT AAAGGCTGCT CCCTACTGGC TGGACGAACC CAAGAACCTT
    ATCCTGGCTC CCGGTGAGGA CGGGAGACTG GTGTGTCGAG CCAATGGGAA CCCCAAGCCC
    ACTGTCCAGT GGATGGTAAA TGGGGAACCA CTGCAATCGG CACCACCCAA CCCAAACCGT
    GAGGTGGCTG GGGACACCAT CATCTTCCGG GACACCCAGA TCAGCAGCAG AGCTGTGTAC
    CAGTGCAACA CCTCCAACGA GCATGGCTAC CTACTGGCCA ACGCCTTCGT CAGCGTGCTG
    GACGTGCCAC CCCGGATGCT GTCACCCCGG AACCAGCTCA TTAGGGTGAT CCTGTACAAC
    CGGACACGGC TGGACTGCCC ATTCTTCGGG TCTCCCATCC CCACACTCCG ATGGTTTAAG
    AACGGGCAGG GAAGTAACCT GGATGGTGGT AACTACCACG TCTACGAGAA TGGCAGTCTG
    GAAATCAAGA TGATCCGCAA AGAGGACCAA GGCATCTACA CCTGTGTCGC CACCAACATC
    CTGGGCAAAG CTGAGAACCA GGTCCGCCTG GAGGTCAAAG ACCCCACCAG GATCTACCGG
    ATGCCTGAAG ACCAGGTGGC CAAAAGGGGC ACGACGGTGC AGCTGGAGTG TCGGGTGAAG
    CACGACCCCT CCCTGAAGCT CACGGTCTCC TGGCTGAAGG ATGATGAGCC TCTCTACATA
    GGAAACAGGA TGAAGAAGGA AGACGACTCC CTGACCATCT TTGGTGTGGC TGAGCGGGAC
    CAGGGCAGTT ACACATGTGT TGCCAGCACT GAGCTGGACC AGGACCTGGC CAAGGCCTAC
    CTCACCGTGC TAGCTGATCA GGCCACTCCA ACTAACCGTT TGGCTGCCCT GCCCAAAGGA
    CGGCCAGACC GACCCCGGGA CTTGGAGCTC ACTGACCTGG CTGAGAGGAG TGTGAGGCTG
    ACCTGGATCC CAGGGGATGA TAACAACAGC CCCATCACAG ACTACGTCGT CCAGTTTGAA
    GAGGACCAGT TCCAGCCAGG GGTCTGGCAT GACCATTCCA AGTTCCCAGG CAGCGTCAAC
    TCTGCCATCC TCCAGTTGTC CCCATATGTC AACTACCAGT TCCGGGTCAT CGCCATCAAC
    GAGGTGGGCA GCAGCCATCC CAGCCTCCCC TCGGAGCGCT ACAGGACCAG CGGGGCGCCC
    CCTGAGTCCA ATCCCGCCGA CGTAAAGGGA GAAGGGACCA GAAAGAACAA CATGGAGATC
    ACTTGGACGC CCATGAACGC CACCTCTGCC TTTGGCCCCA ACCTGCGCTA CATCGTCAAG
    TGGCGGCGGA GAGAAACTCG AGAGACGTGG AACAACGCCA CAGTGTGGGG CTCCCGCTAC
    GTGGTGGGCC AGACCCCTGT CTACGTGCCC TATGAGATCC GAGTCCAGGC CGAAAATGAC
    TTTGGGAAGG GCCCTGAGCC AGACACTGTC ATCGGTTACT CCGGGGAAGA CTATCCCAGG
    GCTGCACCCA CTGACGTTAA AATCCGAGTC CTGAACAGCA CAGCCATCAG TCTCCAGTGG
    AACCGCGTTT ACCCTGACAC AGTCCAGGGC CAGCTCAGAG AGTACCGAGC CTACTACTGG
    AGGGAGAGCA GCTTGCTGAA GAACCTGTGG GTGTCTCAGA AGAGACAGCA AGCCAGCTTC
    CCTGGTGACC GCCCCCGCGG CGTGGTGTCC CGCCTCTTCC CCTACAGTAA CTACAAGCTG
    GAGATGGTCG TGGTCAATGG GAGAGGCGAT GGGCCTCGCA GTGAAACCAA GGAGTTCACC
    ACCCCGGAAG GAGTACCCAG TGCCCCCAGA CGTTTCCGAG TCCGGCAGCC CAACCTGGAG
    ACAATCAACC TGGAATGGGA TCATCCGGAA CACCCCAATG GGATCCTGAT CGGATACACT
    CTCAAATATG TGGCTTTTAA TGGAACCAAA TTAGGAAAGC AGATAGTGGA AAACTTCTCT
    CCCAATCAGA CCAAGTTCAC GATGCAAAGA GCAGACCCCG TGTCGCGCTA CCGCTTTACC
    CTCAGTGCCA GGACGCAGGT GGGCTCTGGG GAAGCAGTCA CAGAGGAGTC ACCAGCACCC
    CCAAATGAAG CTACTCCAAC AGCAGCTCCT CCCACGTTGC CCCCGACCAC GGTGAGTGTG
    ACGGGCGTTG TGAGCAGTAC AGACGCTACT GCCACCGCTG CCACCACCGA AGCCACAACA
    GTCCCCACCA TCCCAAGTGT CGCACCTACC ACCATGGCCA CCACCACCGT CGCCACAACT
    ACAACCACTG CCGCCACCAC CATGGAGAGG CCTCCCACCA CCACCTCCAA GACTAAGATG
    CCCGAATCCG CCCCCGACAG GCAGTCCATA TGGAACGTCA CGGTGCTCCC CAACAGTAAA
    TGGGCCAACA TCACCTGGAG ACACAATCTC GGGCCCGGAA CTGCCTTTGT GGTTGAGTAC
    ATCGACAGCA ACCATACGAA AAAAACTGTC CCTGTTAAGA GCCAGGTCCA GCCCGTACAG
    CTGACAGACC TCTATCCCGG GATGACATAC ACGTTGCGCG TTTATTCCCG GGACAAGGAG
    GGCATCAGCA GTACCGTCAT CACCTTTATG ACCAGTACAG CTTACACCAA TAACCAGGCA
    GACATCGCCA CCCAGGGCTG GTTCATTGGG CTCATGTGTG CCATCGCCCT CCTGGTGCTG
    ATCCTGCTCA TCGTCTGCTT CATCAAGAGG AGTCGAGGTG GCAAATACCC AGTGCGAGAA
    AAGAAGGACG TTCCTCTTGG CCCCGAAGAC CCCAAGGAAG AGGATGGCTC CTTTGACTAC
    AGTGACGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACGT CCCTCGATGG TACCATCAAG
    CAGCAAGAGA GCGACGACAG CCTGGTGGAC TATGGCGAGG GTGGCGAGGG GCAGTTCAAT
    GAGGATGGGT CCTTCATCGG CCAGTACACG GTCAAAAAGG ACAAAGAGGA GACAGAGGGC
    AATGAAAGCT CAGAGGCCAC ATCGCCTGTC AACGCCATCT ATTCTCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013966652.1
    CDS303..4316
    Translation

    Target ORF information:

    RefSeq Version XM_014111177.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111177.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CTATCCCAGC CCCCAACCAT CACCAAGCAG
    TCAGTGAAGG ACCACATCGT GGATCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAG
    GGGAACCCTG CCCCCAGCTT CCACTGGACT CGAAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCAATGAG GAGAAAGTCT GGGACCCTGG TGATCGACTT CCGTAGTGGT
    GGGCGGCCCG AAGAATATGA AGGGGAATAC CAGTGCTTTG CCCGCAACAA ATTTGGCACG
    GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAGTCTC CCCTGTGGCC CAAGGAGAAC
    CTAGACCCAG TCGTGGTTCA AGAAGGTGCT CCTTTGACAC TCCAGTGCAA CCCTCCACCC
    GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACTCAGGAC
    AAGCGTGTCT CTCAGGGCCA CAACGGTGAC CTGTATTTCT CCAACGTGAT GCTTCAGGAT
    ATGCAGACAG ACTACAGCTG CAACGCGCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCACA CGAGGAGTTG CAGAAAGGAC ACCCAGCTTC
    ATGTATCCCC AGGGCACAGC GAGCAGTCAG ATGGTGCTGC GTGGCACGGA TCTTCTGCTG
    GAGTGCATCG CCTCTGGGGT CCCCACGCCA GACATCGCAT GGTACAAGAA AGGTGGGGAC
    CTCCCATCTG ACAAGGCCAA GTTTGAGAAC TTTAACAAGG CTCTGCGTAT CACCAACGTC
    TCCGAGGAAG ACTCTGGGGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG
    CACACGATCT CGGTGAGAGT AAAGGCTGCT CCCTACTGGC TGGACGAACC CAAGAACCTT
    ATCCTGGCTC CCGGTGAGGA CGGGAGACTG GTGTGTCGAG CCAATGGGAA CCCCAAGCCC
    ACTGTCCAGT GGATGGTAAA TGGGGAACCA CTGCAATCGG CACCACCCAA CCCAAACCGT
    GAGGTGGCTG GGGACACCAT CATCTTCCGG GACACCCAGA TCAGCAGCAG AGCTGTGTAC
    CAGTGCAACA CCTCCAACGA GCATGGCTAC CTACTGGCCA ACGCCTTCGT CAGCGTGCTG
    GACGTGCCAC CCCGGATGCT GTCACCCCGG AACCAGCTCA TTAGGGTGAT CCTGTACAAC
    CGGACACGGC TGGACTGCCC ATTCTTCGGG TCTCCCATCC CCACACTCCG ATGGTTTAAG
    AACGGGCAGG GAAGTAACCT GGATGGTGGT AACTACCACG TCTACGAGAA TGGCAGTCTG
    GAAATCAAGA TGATCCGCAA AGAGGACCAA GGCATCTACA CCTGTGTCGC CACCAACATC
    CTGGGCAAAG CTGAGAACCA GGTCCGCCTG GAGGTCAAAG ACCCCACCAG GATCTACCGG
    ATGCCTGAAG ACCAGGTGGC CAAAAGGGGC ACGACGGTGC AGCTGGAGTG TCGGGTGAAG
    CACGACCCCT CCCTGAAGCT CACGGTCTCC TGGCTGAAGG ATGATGAGCC TCTCTACATA
    GGAAACAGGA TGAAGAAGGA AGACGACTCC CTGACCATCT TTGGTGTGGC TGAGCGGGAC
    CAGGGCAGTT ACACATGTGT TGCCAGCACT GAGCTGGACC AGGACCTGGC CAAGGCCTAC
    CTCACCGTGC TAGCTGATCA GGCCACTCCA ACTAACCGTT TGGCTGCCCT GCCCAAAGGA
    CGGCCAGACC GACCCCGGGA CTTGGAGCTC ACTGACCTGG CTGAGAGGAG TGTGAGGCTG
    ACCTGGATCC CAGGGGATGA TAACAACAGC CCCATCACAG ACTACGTCGT CCAGTTTGAA
    GAGGACCAGT TCCAGCCAGG GGTCTGGCAT GACCATTCCA AGTTCCCAGG CAGCGTCAAC
    TCTGCCATCC TCCAGTTGTC CCCATATGTC AACTACCAGT TCCGGGTCAT CGCCATCAAC
    GAGGTGGGCA GCAGCCATCC CAGCCTCCCC TCGGAGCGCT ACAGGACCAG CGGGGCGCCC
    CCTGAGTCCA ATCCCGCCGA CGTAAAGGGA GAAGGGACCA GAAAGAACAA CATGGAGATC
    ACTTGGACGC CCATGAACGC CACCTCTGCC TTTGGCCCCA ACCTGCGCTA CATCGTCAAG
    TGGCGGCGGA GAGAAACTCG AGAGACGTGG AACAACGCCA CAGTGTGGGG CTCCCGCTAC
    GTGGTGGGCC AGACCCCTGT CTACGTGCCC TATGAGATCC GAGTCCAGGC CGAAAATGAC
    TTTGGGAAGG GCCCTGAGCC AGACACTGTC ATCGGTTACT CCGGGGAAGA CTATCCCAGG
    GCTGCACCCA CTGACGTTAA AATCCGAGTC CTGAACAGCA CAGCCATCAG TCTCCAGTGG
    AACCGCGTTT ACCCTGACAC AGTCCAGGGC CAGCTCAGAG AGTACCGAGC CTACTACTGG
    AGGGAGAGCA GCTTGCTGAA GAACCTGTGG GTGTCTCAGA AGAGACAGCA AGCCAGCTTC
    CCTGGTGACC GCCCCCGCGG CGTGGTGTCC CGCCTCTTCC CCTACAGTAA CTACAAGCTG
    GAGATGGTCG TGGTCAATGG GAGAGGCGAT GGGCCTCGCA GTGAAACCAA GGAGTTCACC
    ACCCCGGAAG GAGTACCCAG TGCCCCCAGA CGTTTCCGAG TCCGGCAGCC CAACCTGGAG
    ACAATCAACC TGGAATGGGA TCATCCGGAA CACCCCAATG GGATCCTGAT CGGATACACT
    CTCAAATATG TGGCTTTTAA TGGAACCAAA TTAGGAAAGC AGATAGTGGA AAACTTCTCT
    CCCAATCAGA CCAAGTTCAC GATGCAAAGA GCAGACCCCG TGTCGCGCTA CCGCTTTACC
    CTCAGTGCCA GGACGCAGGT GGGCTCTGGG GAAGCAGTCA CAGAGGAGTC ACCAGCACCC
    CCAAATGAAG CTACTCCAAC AGCAGCTCCT CCCACGTTGC CCCCGACCAC GGTGAGTGTG
    ACGGGCGTTG TGAGCAGTAC AGACGCTACT GCCACCGCTG CCACCACCGA AGCCACAACA
    GTCCCCACCA TCCCAAGTGT CGCACCTACC ACCATGGCCA CCACCACCGT CGCCACAACT
    ACAACCACTG CCGCCACCAC CATGGAGAGG CCTCCCACCA CCACCTCCAA GACTAAGATG
    CCCGAATCCG CCCCCGACAG GCAGTCCATA TGGAACGTCA CGGTGCTCCC CAACAGTAAA
    TGGGCCAACA TCACCTGGAG ACACAATCTC GGGCCCGGAA CTGCCTTTGT GGTTGAGTAC
    ATCGACAGCA ACCATACGAA AAAAACTGTC CCTGTTAAGA GCCAGGTCCA GCCCGTACAG
    CTGACAGACC TCTATCCCGG GATGACATAC ACGTTGCGCG TTTATTCCCG GGACAAGGAG
    GGCATCAGCA GTACCGTCAT CACCTTTATG ACCAGTACAG CTTACACCAA TAACCAGGCA
    GACATCGCCA CCCAGGGCTG GTTCATTGGG CTCATGTGTG CCATCGCCCT CCTGGTGCTG
    ATCCTGCTCA TCGTCTGCTT CATCAAGAGG AGTCGAGGTG GCAAATACCC AGTGCGAGAA
    AAGAAGGACG TTCCTCTTGG CCCCGAAGAC CCCAAGGAAG AGGATGGCTC CTTTGACTAC
    AGTGACGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACGT CCCTCGATGG TACCATCAAG
    CAGCAAGAGA GCGACGACAG CCTGGTGGAC TATGGCGAGG GTGGCGAGGG GCAGTTCAAT
    GAGGATGGGT CCTTCATCGG CCAGTACACG GTCAAAAAGG ACAAAGAGGA GACAGAGGGC
    AATGAAAGCT CAGAGGCCAC ATCGCCTGTC AACGCCATCT ATTCTCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45313
    Clone ID Related Accession (Same CDS sequence) XM_005640777.3 , XM_005640777.2
    Accession Version XM_005640777.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3858bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurofascin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005640777.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TACTCCAACA GCAGCCCCCG ACAGGCAGTC CATATGGAAC
    GTCACGGTGC TCCCCAACAG TAAATGGGCC AACATCACCT GGAGACACAA TCTCGGGCCC
    GGAACTGCCT TTGTGGTTGA GTACATCGAC AGCAACCATA CGAAAAAAAC TGTCCCTGTT
    AAGAGCCAGG TCCAGCCCGT ACAGCTGACA GACCTCTATC CCGGGATGAC ATACACGTTG
    CGCGTTTATT CCCGGGACAA GGAGGGCATC AGCAGTACCG TCATCACCTT TATGACCAGT
    ACAGCTTACA CCAATAACCA GGCAGACATC GCCACCCAGG GCTGGTTCAT TGGGCTCATG
    TGTGCCATCG CCCTCCTGGT GCTGATCCTG CTCATCGTCT GCTTCATCAA GAGGAGTCGA
    GGTGGCAAAT ACCCAGTGCG AGAAAAGAAG GACGTTCCTC TTGGCCCCGA AGACCCCAAG
    GAAGAGGATG GCTCCTTTGA CTACAGTGAC GAGGACAACA AGCCCCTGCA GGGCAGCCAG
    ACGTCCCTCG ATGGTACCAT CAAGCAGCAA GAGAGCGACG ACAGCCTGGT GGACTATGGC
    GAGGGTGGCG AGGGGCAGTT CAATGAGGAT GGGTCCTTCA TCGGCCAGTA CACGGTCAAA
    AAGGACAAAG AGGAGACAGA GGGCAATGAA AGCTCAGAGG CCACATCGCC TGTCAACGCC
    ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640834.2
    CDS303..4160
    Translation

    Target ORF information:

    RefSeq Version XM_005640777.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640777.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TACTCCAACA GCAGCCCCCG ACAGGCAGTC CATATGGAAC
    GTCACGGTGC TCCCCAACAG TAAATGGGCC AACATCACCT GGAGACACAA TCTCGGGCCC
    GGAACTGCCT TTGTGGTTGA GTACATCGAC AGCAACCATA CGAAAAAAAC TGTCCCTGTT
    AAGAGCCAGG TCCAGCCCGT ACAGCTGACA GACCTCTATC CCGGGATGAC ATACACGTTG
    CGCGTTTATT CCCGGGACAA GGAGGGCATC AGCAGTACCG TCATCACCTT TATGACCAGT
    ACAGCTTACA CCAATAACCA GGCAGACATC GCCACCCAGG GCTGGTTCAT TGGGCTCATG
    TGTGCCATCG CCCTCCTGGT GCTGATCCTG CTCATCGTCT GCTTCATCAA GAGGAGTCGA
    GGTGGCAAAT ACCCAGTGCG AGAAAAGAAG GACGTTCCTC TTGGCCCCGA AGACCCCAAG
    GAAGAGGATG GCTCCTTTGA CTACAGTGAC GAGGACAACA AGCCCCTGCA GGGCAGCCAG
    ACGTCCCTCG ATGGTACCAT CAAGCAGCAA GAGAGCGACG ACAGCCTGGT GGACTATGGC
    GAGGGTGGCG AGGGGCAGTT CAATGAGGAT GGGTCCTTCA TCGGCCAGTA CACGGTCAAA
    AAGGACAAAG AGGAGACAGA GGGCAATGAA AGCTCAGAGG CCACATCGCC TGTCAACGCC
    ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45313
    Clone ID Related Accession (Same CDS sequence) XM_005640777.3 , XM_005640777.2
    Accession Version XM_005640777.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3858bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neurofascin isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640777.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TACTCCAACA GCAGCCCCCG ACAGGCAGTC CATATGGAAC
    GTCACGGTGC TCCCCAACAG TAAATGGGCC AACATCACCT GGAGACACAA TCTCGGGCCC
    GGAACTGCCT TTGTGGTTGA GTACATCGAC AGCAACCATA CGAAAAAAAC TGTCCCTGTT
    AAGAGCCAGG TCCAGCCCGT ACAGCTGACA GACCTCTATC CCGGGATGAC ATACACGTTG
    CGCGTTTATT CCCGGGACAA GGAGGGCATC AGCAGTACCG TCATCACCTT TATGACCAGT
    ACAGCTTACA CCAATAACCA GGCAGACATC GCCACCCAGG GCTGGTTCAT TGGGCTCATG
    TGTGCCATCG CCCTCCTGGT GCTGATCCTG CTCATCGTCT GCTTCATCAA GAGGAGTCGA
    GGTGGCAAAT ACCCAGTGCG AGAAAAGAAG GACGTTCCTC TTGGCCCCGA AGACCCCAAG
    GAAGAGGATG GCTCCTTTGA CTACAGTGAC GAGGACAACA AGCCCCTGCA GGGCAGCCAG
    ACGTCCCTCG ATGGTACCAT CAAGCAGCAA GAGAGCGACG ACAGCCTGGT GGACTATGGC
    GAGGGTGGCG AGGGGCAGTT CAATGAGGAT GGGTCCTTCA TCGGCCAGTA CACGGTCAAA
    AAGGACAAAG AGGAGACAGA GGGCAATGAA AGCTCAGAGG CCACATCGCC TGTCAACGCC
    ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640834.2
    CDS288..4145
    Translation

    Target ORF information:

    RefSeq Version XM_005640777.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640777.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TACTCCAACA GCAGCCCCCG ACAGGCAGTC CATATGGAAC
    GTCACGGTGC TCCCCAACAG TAAATGGGCC AACATCACCT GGAGACACAA TCTCGGGCCC
    GGAACTGCCT TTGTGGTTGA GTACATCGAC AGCAACCATA CGAAAAAAAC TGTCCCTGTT
    AAGAGCCAGG TCCAGCCCGT ACAGCTGACA GACCTCTATC CCGGGATGAC ATACACGTTG
    CGCGTTTATT CCCGGGACAA GGAGGGCATC AGCAGTACCG TCATCACCTT TATGACCAGT
    ACAGCTTACA CCAATAACCA GGCAGACATC GCCACCCAGG GCTGGTTCAT TGGGCTCATG
    TGTGCCATCG CCCTCCTGGT GCTGATCCTG CTCATCGTCT GCTTCATCAA GAGGAGTCGA
    GGTGGCAAAT ACCCAGTGCG AGAAAAGAAG GACGTTCCTC TTGGCCCCGA AGACCCCAAG
    GAAGAGGATG GCTCCTTTGA CTACAGTGAC GAGGACAACA AGCCCCTGCA GGGCAGCCAG
    ACGTCCCTCG ATGGTACCAT CAAGCAGCAA GAGAGCGACG ACAGCCTGGT GGACTATGGC
    GAGGGTGGCG AGGGGCAGTT CAATGAGGAT GGGTCCTTCA TCGGCCAGTA CACGGTCAAA
    AAGGACAAAG AGGAGACAGA GGGCAATGAA AGCTCAGAGG CCACATCGCC TGTCAACGCC
    ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45314
    Clone ID Related Accession (Same CDS sequence) XM_005640778.3 , XM_005640778.2
    Accession Version XM_005640778.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurofascin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005640778.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TACTCCAACA GCAGCTCCTC CCACGTTGCC CCCGACCACG
    GTGAGTGTGA CGGGCGTTGT GAGCAGTACA GACGCTACTG CCACCGCTGC CACCACCGAA
    GCCACAACAG TCCCCACCAT CCCAAGTGTC GCACCTACCA CCATGGCCAC CACCACCGTC
    GCCACAACTA CAACCACTGC CGCCACCACC ATGGAGAGGC CTCCCACCAC CACCTCCAAG
    ACTAAGATGC CCGAATCCGC TTACACCAAT AACCAGGCAG ACATCGCCAC CCAGGGCTGG
    TTCATTGGGC TCATGTGTGC CATCGCCCTC CTGGTGCTGA TCCTGCTCAT CGTCTGCTTC
    ATCAAGAGGA GTCGAGGTGG CAAATACCCA GTGCGAGAAA AGAAGGACGT TCCTCTTGGC
    CCCGAAGACC CCAAGGAAGA GGATGGCTCC TTTGACTACA GTGACGAGGA CAACAAGCCC
    CTGCAGGGCA GCCAGACGTC CCTCGATGGT ACCATCAAGC AGCAAGAGAG CGACGACAGC
    CTGGTGGACT ATGGCGAGGG TGGCGAGGGG CAGTTCAATG AGGATGGGTC CTTCATCGGC
    CAGTACACGG TCAAAAAGGA CAAAGAGGAG ACAGAGGGCA ATGAAAGCTC AGAGGCCACA
    TCGCCTGTCA ACGCCATCTA TTCTCTGGCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640835.2
    CDS303..4115
    Translation

    Target ORF information:

    RefSeq Version XM_005640778.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640778.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TACTCCAACA GCAGCTCCTC CCACGTTGCC CCCGACCACG
    GTGAGTGTGA CGGGCGTTGT GAGCAGTACA GACGCTACTG CCACCGCTGC CACCACCGAA
    GCCACAACAG TCCCCACCAT CCCAAGTGTC GCACCTACCA CCATGGCCAC CACCACCGTC
    GCCACAACTA CAACCACTGC CGCCACCACC ATGGAGAGGC CTCCCACCAC CACCTCCAAG
    ACTAAGATGC CCGAATCCGC TTACACCAAT AACCAGGCAG ACATCGCCAC CCAGGGCTGG
    TTCATTGGGC TCATGTGTGC CATCGCCCTC CTGGTGCTGA TCCTGCTCAT CGTCTGCTTC
    ATCAAGAGGA GTCGAGGTGG CAAATACCCA GTGCGAGAAA AGAAGGACGT TCCTCTTGGC
    CCCGAAGACC CCAAGGAAGA GGATGGCTCC TTTGACTACA GTGACGAGGA CAACAAGCCC
    CTGCAGGGCA GCCAGACGTC CCTCGATGGT ACCATCAAGC AGCAAGAGAG CGACGACAGC
    CTGGTGGACT ATGGCGAGGG TGGCGAGGGG CAGTTCAATG AGGATGGGTC CTTCATCGGC
    CAGTACACGG TCAAAAAGGA CAAAGAGGAG ACAGAGGGCA ATGAAAGCTC AGAGGCCACA
    TCGCCTGTCA ACGCCATCTA TTCTCTGGCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45314
    Clone ID Related Accession (Same CDS sequence) XM_005640778.3 , XM_005640778.2
    Accession Version XM_005640778.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neurofascin isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640778.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TACTCCAACA GCAGCTCCTC CCACGTTGCC CCCGACCACG
    GTGAGTGTGA CGGGCGTTGT GAGCAGTACA GACGCTACTG CCACCGCTGC CACCACCGAA
    GCCACAACAG TCCCCACCAT CCCAAGTGTC GCACCTACCA CCATGGCCAC CACCACCGTC
    GCCACAACTA CAACCACTGC CGCCACCACC ATGGAGAGGC CTCCCACCAC CACCTCCAAG
    ACTAAGATGC CCGAATCCGC TTACACCAAT AACCAGGCAG ACATCGCCAC CCAGGGCTGG
    TTCATTGGGC TCATGTGTGC CATCGCCCTC CTGGTGCTGA TCCTGCTCAT CGTCTGCTTC
    ATCAAGAGGA GTCGAGGTGG CAAATACCCA GTGCGAGAAA AGAAGGACGT TCCTCTTGGC
    CCCGAAGACC CCAAGGAAGA GGATGGCTCC TTTGACTACA GTGACGAGGA CAACAAGCCC
    CTGCAGGGCA GCCAGACGTC CCTCGATGGT ACCATCAAGC AGCAAGAGAG CGACGACAGC
    CTGGTGGACT ATGGCGAGGG TGGCGAGGGG CAGTTCAATG AGGATGGGTC CTTCATCGGC
    CAGTACACGG TCAAAAAGGA CAAAGAGGAG ACAGAGGGCA ATGAAAGCTC AGAGGCCACA
    TCGCCTGTCA ACGCCATCTA TTCTCTGGCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640835.2
    CDS287..4099
    Translation

    Target ORF information:

    RefSeq Version XM_005640778.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640778.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TACTCCAACA GCAGCTCCTC CCACGTTGCC CCCGACCACG
    GTGAGTGTGA CGGGCGTTGT GAGCAGTACA GACGCTACTG CCACCGCTGC CACCACCGAA
    GCCACAACAG TCCCCACCAT CCCAAGTGTC GCACCTACCA CCATGGCCAC CACCACCGTC
    GCCACAACTA CAACCACTGC CGCCACCACC ATGGAGAGGC CTCCCACCAC CACCTCCAAG
    ACTAAGATGC CCGAATCCGC TTACACCAAT AACCAGGCAG ACATCGCCAC CCAGGGCTGG
    TTCATTGGGC TCATGTGTGC CATCGCCCTC CTGGTGCTGA TCCTGCTCAT CGTCTGCTTC
    ATCAAGAGGA GTCGAGGTGG CAAATACCCA GTGCGAGAAA AGAAGGACGT TCCTCTTGGC
    CCCGAAGACC CCAAGGAAGA GGATGGCTCC TTTGACTACA GTGACGAGGA CAACAAGCCC
    CTGCAGGGCA GCCAGACGTC CCTCGATGGT ACCATCAAGC AGCAAGAGAG CGACGACAGC
    CTGGTGGACT ATGGCGAGGG TGGCGAGGGG CAGTTCAATG AGGATGGGTC CTTCATCGGC
    CAGTACACGG TCAAAAAGGA CAAAGAGGAG ACAGAGGGCA ATGAAAGCTC AGAGGCCACA
    TCGCCTGTCA ACGCCATCTA TTCTCTGGCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45315
    Clone ID Related Accession (Same CDS sequence) XM_005640779.2 , XM_005640779.3
    Accession Version XM_005640779.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3762bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neurofascin isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640779.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TTACCCAGTG CCCCCAGACG TTTCCGAGTC CGGCAGCCCA ACCTGGAGAC AATCAACCTG
    GAATGGGATC ATCCGGAACA CCCCAATGGG ATCCTGATCG GATACACTCT CAAATATGTG
    GCTTTTAATG GAACCAAATT AGGAAAGCAG ATAGTGGAAA ACTTCTCTCC CAATCAGACC
    AAGTTCACGA TGCAAAGAGC AGACCCCGTG TCGCGCTACC GCTTTACCCT CAGTGCCAGG
    ACGCAGGTGG GCTCTGGGGA AGCAGTCACA GAGGAGTCAC CAGCACCCCC AAATGAAGCT
    ACTCCAACAG CAGCTCCTCC CACGTTGCCC CCGACCACGG TGAGTGTGAC GGGCGTTGTG
    AGCAGTACAG ACGCTACTGC CACCGCTGCC ACCACCGAAG CCACAACAGT CCCCACCATC
    CCAAGTGTCG CACCTACCAC CATGGCCACC ACCACCGTCG CCACAACTAC AACCACTGCC
    GCCACCACCA TGGAGAGGCC TCCCACCACC ACCTCCAAGA CTAAGATGCC CGAATCCGCC
    CCCGACAGGC AGTCCATATG GAACGTCACG GTGCTCCCCA ACAGTAAATG GGCCAACATC
    ACCTGGAGAC ACAATCTCGG GCCCGGAACT GCCTTTGTGG TTGAGTACAT CGACAGCAAC
    CATACGAAAA AAACTGTCCC TGTTAAGAGC CAGGTCCAGC CCGTACAGCT GACAGACCTC
    TATCCCGGGA TGACATACAC GTTGCGCGTT TATTCCCGGG ACAAGGAGGG CATCAGCAGT
    ACCGTCATCA CCTTTATGAC CAGTACAGCT TACACCAATA ACCAGGCAGA CATCGCCACC
    CAGGGCTGGT TCATTGGGCT CATGTGTGCC ATCGCCCTCC TGGTGCTGAT CCTGCTCATC
    GTCTGCTTCA TCAAGAGGAG TCGAGGTGGC AAATACCCAG TGCGAGAAAA GAAGGACGTT
    CCTCTTGGCC CCGAAGACCC CAAGGAAGAG GATGGCTCCT TTGACTACAG TGACGAGGAC
    AACAAGCCCC TGCAGGGCAG CCAGACGTCC CTCGATGGTA CCATCAAGCA GCAAGAGAGC
    GACGACAGCC TGGTGGACTA TGGCGAGGGT GGCGAGGGGC AGTTCAATGA GGATGGGTCC
    TTCATCGGCC AGTACACGGT CAAAAAGGAC AAAGAGGAGA CAGAGGGCAA TGAAAGCTCA
    GAGGCCACAT CGCCTGTCAA CGCCATCTAT TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640836.2
    CDS287..4048
    Translation

    Target ORF information:

    RefSeq Version XM_005640779.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640779.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TTACCCAGTG CCCCCAGACG TTTCCGAGTC CGGCAGCCCA ACCTGGAGAC AATCAACCTG
    GAATGGGATC ATCCGGAACA CCCCAATGGG ATCCTGATCG GATACACTCT CAAATATGTG
    GCTTTTAATG GAACCAAATT AGGAAAGCAG ATAGTGGAAA ACTTCTCTCC CAATCAGACC
    AAGTTCACGA TGCAAAGAGC AGACCCCGTG TCGCGCTACC GCTTTACCCT CAGTGCCAGG
    ACGCAGGTGG GCTCTGGGGA AGCAGTCACA GAGGAGTCAC CAGCACCCCC AAATGAAGCT
    ACTCCAACAG CAGCTCCTCC CACGTTGCCC CCGACCACGG TGAGTGTGAC GGGCGTTGTG
    AGCAGTACAG ACGCTACTGC CACCGCTGCC ACCACCGAAG CCACAACAGT CCCCACCATC
    CCAAGTGTCG CACCTACCAC CATGGCCACC ACCACCGTCG CCACAACTAC AACCACTGCC
    GCCACCACCA TGGAGAGGCC TCCCACCACC ACCTCCAAGA CTAAGATGCC CGAATCCGCC
    CCCGACAGGC AGTCCATATG GAACGTCACG GTGCTCCCCA ACAGTAAATG GGCCAACATC
    ACCTGGAGAC ACAATCTCGG GCCCGGAACT GCCTTTGTGG TTGAGTACAT CGACAGCAAC
    CATACGAAAA AAACTGTCCC TGTTAAGAGC CAGGTCCAGC CCGTACAGCT GACAGACCTC
    TATCCCGGGA TGACATACAC GTTGCGCGTT TATTCCCGGG ACAAGGAGGG CATCAGCAGT
    ACCGTCATCA CCTTTATGAC CAGTACAGCT TACACCAATA ACCAGGCAGA CATCGCCACC
    CAGGGCTGGT TCATTGGGCT CATGTGTGCC ATCGCCCTCC TGGTGCTGAT CCTGCTCATC
    GTCTGCTTCA TCAAGAGGAG TCGAGGTGGC AAATACCCAG TGCGAGAAAA GAAGGACGTT
    CCTCTTGGCC CCGAAGACCC CAAGGAAGAG GATGGCTCCT TTGACTACAG TGACGAGGAC
    AACAAGCCCC TGCAGGGCAG CCAGACGTCC CTCGATGGTA CCATCAAGCA GCAAGAGAGC
    GACGACAGCC TGGTGGACTA TGGCGAGGGT GGCGAGGGGC AGTTCAATGA GGATGGGTCC
    TTCATCGGCC AGTACACGGT CAAAAAGGAC AAAGAGGAGA CAGAGGGCAA TGAAAGCTCA
    GAGGCCACAT CGCCTGTCAA CGCCATCTAT TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45315
    Clone ID Related Accession (Same CDS sequence) XM_005640779.2 , XM_005640779.3
    Accession Version XM_005640779.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3762bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurofascin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005640779.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TTACCCAGTG CCCCCAGACG TTTCCGAGTC CGGCAGCCCA ACCTGGAGAC AATCAACCTG
    GAATGGGATC ATCCGGAACA CCCCAATGGG ATCCTGATCG GATACACTCT CAAATATGTG
    GCTTTTAATG GAACCAAATT AGGAAAGCAG ATAGTGGAAA ACTTCTCTCC CAATCAGACC
    AAGTTCACGA TGCAAAGAGC AGACCCCGTG TCGCGCTACC GCTTTACCCT CAGTGCCAGG
    ACGCAGGTGG GCTCTGGGGA AGCAGTCACA GAGGAGTCAC CAGCACCCCC AAATGAAGCT
    ACTCCAACAG CAGCTCCTCC CACGTTGCCC CCGACCACGG TGAGTGTGAC GGGCGTTGTG
    AGCAGTACAG ACGCTACTGC CACCGCTGCC ACCACCGAAG CCACAACAGT CCCCACCATC
    CCAAGTGTCG CACCTACCAC CATGGCCACC ACCACCGTCG CCACAACTAC AACCACTGCC
    GCCACCACCA TGGAGAGGCC TCCCACCACC ACCTCCAAGA CTAAGATGCC CGAATCCGCC
    CCCGACAGGC AGTCCATATG GAACGTCACG GTGCTCCCCA ACAGTAAATG GGCCAACATC
    ACCTGGAGAC ACAATCTCGG GCCCGGAACT GCCTTTGTGG TTGAGTACAT CGACAGCAAC
    CATACGAAAA AAACTGTCCC TGTTAAGAGC CAGGTCCAGC CCGTACAGCT GACAGACCTC
    TATCCCGGGA TGACATACAC GTTGCGCGTT TATTCCCGGG ACAAGGAGGG CATCAGCAGT
    ACCGTCATCA CCTTTATGAC CAGTACAGCT TACACCAATA ACCAGGCAGA CATCGCCACC
    CAGGGCTGGT TCATTGGGCT CATGTGTGCC ATCGCCCTCC TGGTGCTGAT CCTGCTCATC
    GTCTGCTTCA TCAAGAGGAG TCGAGGTGGC AAATACCCAG TGCGAGAAAA GAAGGACGTT
    CCTCTTGGCC CCGAAGACCC CAAGGAAGAG GATGGCTCCT TTGACTACAG TGACGAGGAC
    AACAAGCCCC TGCAGGGCAG CCAGACGTCC CTCGATGGTA CCATCAAGCA GCAAGAGAGC
    GACGACAGCC TGGTGGACTA TGGCGAGGGT GGCGAGGGGC AGTTCAATGA GGATGGGTCC
    TTCATCGGCC AGTACACGGT CAAAAAGGAC AAAGAGGAGA CAGAGGGCAA TGAAAGCTCA
    GAGGCCACAT CGCCTGTCAA CGCCATCTAT TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640836.2
    CDS303..4064
    Translation

    Target ORF information:

    RefSeq Version XM_005640779.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640779.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TTACCCAGTG CCCCCAGACG TTTCCGAGTC CGGCAGCCCA ACCTGGAGAC AATCAACCTG
    GAATGGGATC ATCCGGAACA CCCCAATGGG ATCCTGATCG GATACACTCT CAAATATGTG
    GCTTTTAATG GAACCAAATT AGGAAAGCAG ATAGTGGAAA ACTTCTCTCC CAATCAGACC
    AAGTTCACGA TGCAAAGAGC AGACCCCGTG TCGCGCTACC GCTTTACCCT CAGTGCCAGG
    ACGCAGGTGG GCTCTGGGGA AGCAGTCACA GAGGAGTCAC CAGCACCCCC AAATGAAGCT
    ACTCCAACAG CAGCTCCTCC CACGTTGCCC CCGACCACGG TGAGTGTGAC GGGCGTTGTG
    AGCAGTACAG ACGCTACTGC CACCGCTGCC ACCACCGAAG CCACAACAGT CCCCACCATC
    CCAAGTGTCG CACCTACCAC CATGGCCACC ACCACCGTCG CCACAACTAC AACCACTGCC
    GCCACCACCA TGGAGAGGCC TCCCACCACC ACCTCCAAGA CTAAGATGCC CGAATCCGCC
    CCCGACAGGC AGTCCATATG GAACGTCACG GTGCTCCCCA ACAGTAAATG GGCCAACATC
    ACCTGGAGAC ACAATCTCGG GCCCGGAACT GCCTTTGTGG TTGAGTACAT CGACAGCAAC
    CATACGAAAA AAACTGTCCC TGTTAAGAGC CAGGTCCAGC CCGTACAGCT GACAGACCTC
    TATCCCGGGA TGACATACAC GTTGCGCGTT TATTCCCGGG ACAAGGAGGG CATCAGCAGT
    ACCGTCATCA CCTTTATGAC CAGTACAGCT TACACCAATA ACCAGGCAGA CATCGCCACC
    CAGGGCTGGT TCATTGGGCT CATGTGTGCC ATCGCCCTCC TGGTGCTGAT CCTGCTCATC
    GTCTGCTTCA TCAAGAGGAG TCGAGGTGGC AAATACCCAG TGCGAGAAAA GAAGGACGTT
    CCTCTTGGCC CCGAAGACCC CAAGGAAGAG GATGGCTCCT TTGACTACAG TGACGAGGAC
    AACAAGCCCC TGCAGGGCAG CCAGACGTCC CTCGATGGTA CCATCAAGCA GCAAGAGAGC
    GACGACAGCC TGGTGGACTA TGGCGAGGGT GGCGAGGGGC AGTTCAATGA GGATGGGTCC
    TTCATCGGCC AGTACACGGT CAAAAAGGAC AAAGAGGAGA CAGAGGGCAA TGAAAGCTCA
    GAGGCCACAT CGCCTGTCAA CGCCATCTAT TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45316
    Clone ID Related Accession (Same CDS sequence) XM_851151.5 , XM_851151.4
    Accession Version XM_851151.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurofascin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_851151.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCACACG AGGAGTTGCA
    GAAAGGACAC CCAGCTTCAT GTATCCCCAG GGCACAGCGA GCAGTCAGAT GGTGCTGCGT
    GGCACGGATC TTCTGCTGGA GTGCATCGCC TCTGGGGTCC CCACGCCAGA CATCGCATGG
    TACAAGAAAG GTGGGGACCT CCCATCTGAC AAGGCCAAGT TTGAGAACTT TAACAAGGCT
    CTGCGTATCA CCAACGTCTC CGAGGAAGAC TCTGGGGAGT ATTTCTGCCT GGCCTCCAAC
    AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG
    GACGAACCCA AGAACCTTAT CCTGGCTCCC GGTGAGGACG GGAGACTGGT GTGTCGAGCC
    AATGGGAACC CCAAGCCCAC TGTCCAGTGG ATGGTAAATG GGGAACCACT GCAATCGGCA
    CCACCCAACC CAAACCGTGA GGTGGCTGGG GACACCATCA TCTTCCGGGA CACCCAGATC
    AGCAGCAGAG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT ACTGGCCAAC
    GCCTTCGTCA GCGTGCTGGA CGTGCCACCC CGGATGCTGT CACCCCGGAA CCAGCTCATT
    AGGGTGATCC TGTACAACCG GACACGGCTG GACTGCCCAT TCTTCGGGTC TCCCATCCCC
    ACACTCCGAT GGTTTAAGAA CGGGCAGGGA AGTAACCTGG ATGGTGGTAA CTACCACGTC
    TACGAGAATG GCAGTCTGGA AATCAAGATG ATCCGCAAAG AGGACCAAGG CATCTACACC
    TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAGAACCAGG TCCGCCTGGA GGTCAAAGAC
    CCCACCAGGA TCTACCGGAT GCCTGAAGAC CAGGTGGCCA AAAGGGGCAC GACGGTGCAG
    CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAGCTCA CGGTCTCCTG GCTGAAGGAT
    GATGAGCCTC TCTACATAGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT
    GGTGTGGCTG AGCGGGACCA GGGCAGTTAC ACATGTGTTG CCAGCACTGA GCTGGACCAG
    GACCTGGCCA AGGCCTACCT CACCGTGCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG
    GCTGCCCTGC CCAAAGGACG GCCAGACCGA CCCCGGGACT TGGAGCTCAC TGACCTGGCT
    GAGAGGAGTG TGAGGCTGAC CTGGATCCCA GGGGATGATA ACAACAGCCC CATCACAGAC
    TACGTCGTCC AGTTTGAAGA GGACCAGTTC CAGCCAGGGG TCTGGCATGA CCATTCCAAG
    TTCCCAGGCA GCGTCAACTC TGCCATCCTC CAGTTGTCCC CATATGTCAA CTACCAGTTC
    CGGGTCATCG CCATCAACGA GGTGGGCAGC AGCCATCCCA GCCTCCCCTC GGAGCGCTAC
    AGGACCAGCG GGGCGCCCCC TGAGTCCAAT CCCGCCGACG TAAAGGGAGA AGGGACCAGA
    AAGAACAACA TGGAGATCAC TTGGACGCCC ATGAACGCCA CCTCTGCCTT TGGCCCCAAC
    CTGCGCTACA TCGTCAAGTG GCGGCGGAGA GAAACTCGAG AGACGTGGAA CAACGCCACA
    GTGTGGGGCT CCCGCTACGT GGTGGGCCAG ACCCCTGTCT ACGTGCCCTA TGAGATCCGA
    GTCCAGGCCG AAAATGACTT TGGGAAGGGC CCTGAGCCAG ACACTGTCAT CGGTTACTCC
    GGGGAAGACT TACCCAGTGC CCCCAGACGT TTCCGAGTCC GGCAGCCCAA CCTGGAGACA
    ATCAACCTGG AATGGGATCA TCCGGAACAC CCCAATGGGA TCCTGATCGG ATACACTCTC
    AAATATGTGG CTTTTAATGG AACCAAATTA GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC
    AATCAGACCA AGTTCACGAT GCAAAGAGCA GACCCCGTGT CGCGCTACCG CTTTACCCTC
    AGTGCCAGGA CGCAGGTGGG CTCTGGGGAA GCAGTCACAG AGGAGTCACC AGCACCCCCA
    AATGAAGCTA CTCCAACAGC AGCTCCTCCC ACGTTGCCCC CGACCACGGT GAGTGTGACG
    GGCGTTGTGA GCAGTACAGA CGCTACTGCC ACCGCTGCCA CCACCGAAGC CACAACAGTC
    CCCACCATCC CAAGTGTCGC ACCTACCACC ATGGCCACCA CCACCGTCGC CACAACTACA
    ACCACTGCCG CCACCACCAT GGAGAGGCCT CCCACCACCA CCTCCAAGAC TAAGATGCCC
    GAATCCGCCC CCGACAGGCA GTCCATATGG AACGTCACGG TGCTCCCCAA CAGTAAATGG
    GCCAACATCA CCTGGAGACA CAATCTCGGG CCCGGAACTG CCTTTGTGGT TGAGTACATC
    GACAGCAACC ATACGAAAAA AACTGTCCCT GTTAAGAGCC AGGTCCAGCC CGTACAGCTG
    ACAGACCTCT ATCCCGGGAT GACATACACG TTGCGCGTTT ATTCCCGGGA CAAGGAGGGC
    ATCAGCAGTA CCGTCATCAC CTTTATGACC AGTACAGCTT ACACCAATAA CCAGGCAGAC
    ATCGCCACCC AGGGCTGGTT CATTGGGCTC ATGTGTGCCA TCGCCCTCCT GGTGCTGATC
    CTGCTCATCG TCTGCTTCAT CAAGAGGAGT CGAGGTGGCA AATACCCAGT GCGAGAAAAG
    AAGGACGTTC CTCTTGGCCC CGAAGACCCC AAGGAAGAGG ATGGCTCCTT TGACTACAGT
    GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TCGATGGTAC CATCAAGCAG
    CAAGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGGCA GTTCAATGAG
    GATGGGTCCT TCATCGGCCA GTACACGGTC AAAAAGGACA AAGAGGAGAC AGAGGGCAAT
    GAAAGCTCAG AGGCCACATC GCCTGTCAAC GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_856244.4
    CDS303..4013
    Translation

    Target ORF information:

    RefSeq Version XM_851151.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_851151.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCACACG AGGAGTTGCA
    GAAAGGACAC CCAGCTTCAT GTATCCCCAG GGCACAGCGA GCAGTCAGAT GGTGCTGCGT
    GGCACGGATC TTCTGCTGGA GTGCATCGCC TCTGGGGTCC CCACGCCAGA CATCGCATGG
    TACAAGAAAG GTGGGGACCT CCCATCTGAC AAGGCCAAGT TTGAGAACTT TAACAAGGCT
    CTGCGTATCA CCAACGTCTC CGAGGAAGAC TCTGGGGAGT ATTTCTGCCT GGCCTCCAAC
    AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG
    GACGAACCCA AGAACCTTAT CCTGGCTCCC GGTGAGGACG GGAGACTGGT GTGTCGAGCC
    AATGGGAACC CCAAGCCCAC TGTCCAGTGG ATGGTAAATG GGGAACCACT GCAATCGGCA
    CCACCCAACC CAAACCGTGA GGTGGCTGGG GACACCATCA TCTTCCGGGA CACCCAGATC
    AGCAGCAGAG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT ACTGGCCAAC
    GCCTTCGTCA GCGTGCTGGA CGTGCCACCC CGGATGCTGT CACCCCGGAA CCAGCTCATT
    AGGGTGATCC TGTACAACCG GACACGGCTG GACTGCCCAT TCTTCGGGTC TCCCATCCCC
    ACACTCCGAT GGTTTAAGAA CGGGCAGGGA AGTAACCTGG ATGGTGGTAA CTACCACGTC
    TACGAGAATG GCAGTCTGGA AATCAAGATG ATCCGCAAAG AGGACCAAGG CATCTACACC
    TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAGAACCAGG TCCGCCTGGA GGTCAAAGAC
    CCCACCAGGA TCTACCGGAT GCCTGAAGAC CAGGTGGCCA AAAGGGGCAC GACGGTGCAG
    CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAGCTCA CGGTCTCCTG GCTGAAGGAT
    GATGAGCCTC TCTACATAGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT
    GGTGTGGCTG AGCGGGACCA GGGCAGTTAC ACATGTGTTG CCAGCACTGA GCTGGACCAG
    GACCTGGCCA AGGCCTACCT CACCGTGCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG
    GCTGCCCTGC CCAAAGGACG GCCAGACCGA CCCCGGGACT TGGAGCTCAC TGACCTGGCT
    GAGAGGAGTG TGAGGCTGAC CTGGATCCCA GGGGATGATA ACAACAGCCC CATCACAGAC
    TACGTCGTCC AGTTTGAAGA GGACCAGTTC CAGCCAGGGG TCTGGCATGA CCATTCCAAG
    TTCCCAGGCA GCGTCAACTC TGCCATCCTC CAGTTGTCCC CATATGTCAA CTACCAGTTC
    CGGGTCATCG CCATCAACGA GGTGGGCAGC AGCCATCCCA GCCTCCCCTC GGAGCGCTAC
    AGGACCAGCG GGGCGCCCCC TGAGTCCAAT CCCGCCGACG TAAAGGGAGA AGGGACCAGA
    AAGAACAACA TGGAGATCAC TTGGACGCCC ATGAACGCCA CCTCTGCCTT TGGCCCCAAC
    CTGCGCTACA TCGTCAAGTG GCGGCGGAGA GAAACTCGAG AGACGTGGAA CAACGCCACA
    GTGTGGGGCT CCCGCTACGT GGTGGGCCAG ACCCCTGTCT ACGTGCCCTA TGAGATCCGA
    GTCCAGGCCG AAAATGACTT TGGGAAGGGC CCTGAGCCAG ACACTGTCAT CGGTTACTCC
    GGGGAAGACT TACCCAGTGC CCCCAGACGT TTCCGAGTCC GGCAGCCCAA CCTGGAGACA
    ATCAACCTGG AATGGGATCA TCCGGAACAC CCCAATGGGA TCCTGATCGG ATACACTCTC
    AAATATGTGG CTTTTAATGG AACCAAATTA GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC
    AATCAGACCA AGTTCACGAT GCAAAGAGCA GACCCCGTGT CGCGCTACCG CTTTACCCTC
    AGTGCCAGGA CGCAGGTGGG CTCTGGGGAA GCAGTCACAG AGGAGTCACC AGCACCCCCA
    AATGAAGCTA CTCCAACAGC AGCTCCTCCC ACGTTGCCCC CGACCACGGT GAGTGTGACG
    GGCGTTGTGA GCAGTACAGA CGCTACTGCC ACCGCTGCCA CCACCGAAGC CACAACAGTC
    CCCACCATCC CAAGTGTCGC ACCTACCACC ATGGCCACCA CCACCGTCGC CACAACTACA
    ACCACTGCCG CCACCACCAT GGAGAGGCCT CCCACCACCA CCTCCAAGAC TAAGATGCCC
    GAATCCGCCC CCGACAGGCA GTCCATATGG AACGTCACGG TGCTCCCCAA CAGTAAATGG
    GCCAACATCA CCTGGAGACA CAATCTCGGG CCCGGAACTG CCTTTGTGGT TGAGTACATC
    GACAGCAACC ATACGAAAAA AACTGTCCCT GTTAAGAGCC AGGTCCAGCC CGTACAGCTG
    ACAGACCTCT ATCCCGGGAT GACATACACG TTGCGCGTTT ATTCCCGGGA CAAGGAGGGC
    ATCAGCAGTA CCGTCATCAC CTTTATGACC AGTACAGCTT ACACCAATAA CCAGGCAGAC
    ATCGCCACCC AGGGCTGGTT CATTGGGCTC ATGTGTGCCA TCGCCCTCCT GGTGCTGATC
    CTGCTCATCG TCTGCTTCAT CAAGAGGAGT CGAGGTGGCA AATACCCAGT GCGAGAAAAG
    AAGGACGTTC CTCTTGGCCC CGAAGACCCC AAGGAAGAGG ATGGCTCCTT TGACTACAGT
    GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TCGATGGTAC CATCAAGCAG
    CAAGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGGCA GTTCAATGAG
    GATGGGTCCT TCATCGGCCA GTACACGGTC AAAAAGGACA AAGAGGAGAC AGAGGGCAAT
    GAAAGCTCAG AGGCCACATC GCCTGTCAAC GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45316
    Clone ID Related Accession (Same CDS sequence) XM_851151.5 , XM_851151.4
    Accession Version XM_851151.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neurofascin isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_851151.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCACACG AGGAGTTGCA
    GAAAGGACAC CCAGCTTCAT GTATCCCCAG GGCACAGCGA GCAGTCAGAT GGTGCTGCGT
    GGCACGGATC TTCTGCTGGA GTGCATCGCC TCTGGGGTCC CCACGCCAGA CATCGCATGG
    TACAAGAAAG GTGGGGACCT CCCATCTGAC AAGGCCAAGT TTGAGAACTT TAACAAGGCT
    CTGCGTATCA CCAACGTCTC CGAGGAAGAC TCTGGGGAGT ATTTCTGCCT GGCCTCCAAC
    AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG
    GACGAACCCA AGAACCTTAT CCTGGCTCCC GGTGAGGACG GGAGACTGGT GTGTCGAGCC
    AATGGGAACC CCAAGCCCAC TGTCCAGTGG ATGGTAAATG GGGAACCACT GCAATCGGCA
    CCACCCAACC CAAACCGTGA GGTGGCTGGG GACACCATCA TCTTCCGGGA CACCCAGATC
    AGCAGCAGAG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT ACTGGCCAAC
    GCCTTCGTCA GCGTGCTGGA CGTGCCACCC CGGATGCTGT CACCCCGGAA CCAGCTCATT
    AGGGTGATCC TGTACAACCG GACACGGCTG GACTGCCCAT TCTTCGGGTC TCCCATCCCC
    ACACTCCGAT GGTTTAAGAA CGGGCAGGGA AGTAACCTGG ATGGTGGTAA CTACCACGTC
    TACGAGAATG GCAGTCTGGA AATCAAGATG ATCCGCAAAG AGGACCAAGG CATCTACACC
    TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAGAACCAGG TCCGCCTGGA GGTCAAAGAC
    CCCACCAGGA TCTACCGGAT GCCTGAAGAC CAGGTGGCCA AAAGGGGCAC GACGGTGCAG
    CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAGCTCA CGGTCTCCTG GCTGAAGGAT
    GATGAGCCTC TCTACATAGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT
    GGTGTGGCTG AGCGGGACCA GGGCAGTTAC ACATGTGTTG CCAGCACTGA GCTGGACCAG
    GACCTGGCCA AGGCCTACCT CACCGTGCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG
    GCTGCCCTGC CCAAAGGACG GCCAGACCGA CCCCGGGACT TGGAGCTCAC TGACCTGGCT
    GAGAGGAGTG TGAGGCTGAC CTGGATCCCA GGGGATGATA ACAACAGCCC CATCACAGAC
    TACGTCGTCC AGTTTGAAGA GGACCAGTTC CAGCCAGGGG TCTGGCATGA CCATTCCAAG
    TTCCCAGGCA GCGTCAACTC TGCCATCCTC CAGTTGTCCC CATATGTCAA CTACCAGTTC
    CGGGTCATCG CCATCAACGA GGTGGGCAGC AGCCATCCCA GCCTCCCCTC GGAGCGCTAC
    AGGACCAGCG GGGCGCCCCC TGAGTCCAAT CCCGCCGACG TAAAGGGAGA AGGGACCAGA
    AAGAACAACA TGGAGATCAC TTGGACGCCC ATGAACGCCA CCTCTGCCTT TGGCCCCAAC
    CTGCGCTACA TCGTCAAGTG GCGGCGGAGA GAAACTCGAG AGACGTGGAA CAACGCCACA
    GTGTGGGGCT CCCGCTACGT GGTGGGCCAG ACCCCTGTCT ACGTGCCCTA TGAGATCCGA
    GTCCAGGCCG AAAATGACTT TGGGAAGGGC CCTGAGCCAG ACACTGTCAT CGGTTACTCC
    GGGGAAGACT TACCCAGTGC CCCCAGACGT TTCCGAGTCC GGCAGCCCAA CCTGGAGACA
    ATCAACCTGG AATGGGATCA TCCGGAACAC CCCAATGGGA TCCTGATCGG ATACACTCTC
    AAATATGTGG CTTTTAATGG AACCAAATTA GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC
    AATCAGACCA AGTTCACGAT GCAAAGAGCA GACCCCGTGT CGCGCTACCG CTTTACCCTC
    AGTGCCAGGA CGCAGGTGGG CTCTGGGGAA GCAGTCACAG AGGAGTCACC AGCACCCCCA
    AATGAAGCTA CTCCAACAGC AGCTCCTCCC ACGTTGCCCC CGACCACGGT GAGTGTGACG
    GGCGTTGTGA GCAGTACAGA CGCTACTGCC ACCGCTGCCA CCACCGAAGC CACAACAGTC
    CCCACCATCC CAAGTGTCGC ACCTACCACC ATGGCCACCA CCACCGTCGC CACAACTACA
    ACCACTGCCG CCACCACCAT GGAGAGGCCT CCCACCACCA CCTCCAAGAC TAAGATGCCC
    GAATCCGCCC CCGACAGGCA GTCCATATGG AACGTCACGG TGCTCCCCAA CAGTAAATGG
    GCCAACATCA CCTGGAGACA CAATCTCGGG CCCGGAACTG CCTTTGTGGT TGAGTACATC
    GACAGCAACC ATACGAAAAA AACTGTCCCT GTTAAGAGCC AGGTCCAGCC CGTACAGCTG
    ACAGACCTCT ATCCCGGGAT GACATACACG TTGCGCGTTT ATTCCCGGGA CAAGGAGGGC
    ATCAGCAGTA CCGTCATCAC CTTTATGACC AGTACAGCTT ACACCAATAA CCAGGCAGAC
    ATCGCCACCC AGGGCTGGTT CATTGGGCTC ATGTGTGCCA TCGCCCTCCT GGTGCTGATC
    CTGCTCATCG TCTGCTTCAT CAAGAGGAGT CGAGGTGGCA AATACCCAGT GCGAGAAAAG
    AAGGACGTTC CTCTTGGCCC CGAAGACCCC AAGGAAGAGG ATGGCTCCTT TGACTACAGT
    GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TCGATGGTAC CATCAAGCAG
    CAAGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGGCA GTTCAATGAG
    GATGGGTCCT TCATCGGCCA GTACACGGTC AAAAAGGACA AAGAGGAGAC AGAGGGCAAT
    GAAAGCTCAG AGGCCACATC GCCTGTCAAC GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_856244.4
    CDS287..3997
    Translation

    Target ORF information:

    RefSeq Version XM_851151.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_851151.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCACACG AGGAGTTGCA
    GAAAGGACAC CCAGCTTCAT GTATCCCCAG GGCACAGCGA GCAGTCAGAT GGTGCTGCGT
    GGCACGGATC TTCTGCTGGA GTGCATCGCC TCTGGGGTCC CCACGCCAGA CATCGCATGG
    TACAAGAAAG GTGGGGACCT CCCATCTGAC AAGGCCAAGT TTGAGAACTT TAACAAGGCT
    CTGCGTATCA CCAACGTCTC CGAGGAAGAC TCTGGGGAGT ATTTCTGCCT GGCCTCCAAC
    AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG
    GACGAACCCA AGAACCTTAT CCTGGCTCCC GGTGAGGACG GGAGACTGGT GTGTCGAGCC
    AATGGGAACC CCAAGCCCAC TGTCCAGTGG ATGGTAAATG GGGAACCACT GCAATCGGCA
    CCACCCAACC CAAACCGTGA GGTGGCTGGG GACACCATCA TCTTCCGGGA CACCCAGATC
    AGCAGCAGAG CTGTGTACCA GTGCAACACC TCCAACGAGC ATGGCTACCT ACTGGCCAAC
    GCCTTCGTCA GCGTGCTGGA CGTGCCACCC CGGATGCTGT CACCCCGGAA CCAGCTCATT
    AGGGTGATCC TGTACAACCG GACACGGCTG GACTGCCCAT TCTTCGGGTC TCCCATCCCC
    ACACTCCGAT GGTTTAAGAA CGGGCAGGGA AGTAACCTGG ATGGTGGTAA CTACCACGTC
    TACGAGAATG GCAGTCTGGA AATCAAGATG ATCCGCAAAG AGGACCAAGG CATCTACACC
    TGTGTCGCCA CCAACATCCT GGGCAAAGCT GAGAACCAGG TCCGCCTGGA GGTCAAAGAC
    CCCACCAGGA TCTACCGGAT GCCTGAAGAC CAGGTGGCCA AAAGGGGCAC GACGGTGCAG
    CTGGAGTGTC GGGTGAAGCA CGACCCCTCC CTGAAGCTCA CGGTCTCCTG GCTGAAGGAT
    GATGAGCCTC TCTACATAGG AAACAGGATG AAGAAGGAAG ACGACTCCCT GACCATCTTT
    GGTGTGGCTG AGCGGGACCA GGGCAGTTAC ACATGTGTTG CCAGCACTGA GCTGGACCAG
    GACCTGGCCA AGGCCTACCT CACCGTGCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG
    GCTGCCCTGC CCAAAGGACG GCCAGACCGA CCCCGGGACT TGGAGCTCAC TGACCTGGCT
    GAGAGGAGTG TGAGGCTGAC CTGGATCCCA GGGGATGATA ACAACAGCCC CATCACAGAC
    TACGTCGTCC AGTTTGAAGA GGACCAGTTC CAGCCAGGGG TCTGGCATGA CCATTCCAAG
    TTCCCAGGCA GCGTCAACTC TGCCATCCTC CAGTTGTCCC CATATGTCAA CTACCAGTTC
    CGGGTCATCG CCATCAACGA GGTGGGCAGC AGCCATCCCA GCCTCCCCTC GGAGCGCTAC
    AGGACCAGCG GGGCGCCCCC TGAGTCCAAT CCCGCCGACG TAAAGGGAGA AGGGACCAGA
    AAGAACAACA TGGAGATCAC TTGGACGCCC ATGAACGCCA CCTCTGCCTT TGGCCCCAAC
    CTGCGCTACA TCGTCAAGTG GCGGCGGAGA GAAACTCGAG AGACGTGGAA CAACGCCACA
    GTGTGGGGCT CCCGCTACGT GGTGGGCCAG ACCCCTGTCT ACGTGCCCTA TGAGATCCGA
    GTCCAGGCCG AAAATGACTT TGGGAAGGGC CCTGAGCCAG ACACTGTCAT CGGTTACTCC
    GGGGAAGACT TACCCAGTGC CCCCAGACGT TTCCGAGTCC GGCAGCCCAA CCTGGAGACA
    ATCAACCTGG AATGGGATCA TCCGGAACAC CCCAATGGGA TCCTGATCGG ATACACTCTC
    AAATATGTGG CTTTTAATGG AACCAAATTA GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC
    AATCAGACCA AGTTCACGAT GCAAAGAGCA GACCCCGTGT CGCGCTACCG CTTTACCCTC
    AGTGCCAGGA CGCAGGTGGG CTCTGGGGAA GCAGTCACAG AGGAGTCACC AGCACCCCCA
    AATGAAGCTA CTCCAACAGC AGCTCCTCCC ACGTTGCCCC CGACCACGGT GAGTGTGACG
    GGCGTTGTGA GCAGTACAGA CGCTACTGCC ACCGCTGCCA CCACCGAAGC CACAACAGTC
    CCCACCATCC CAAGTGTCGC ACCTACCACC ATGGCCACCA CCACCGTCGC CACAACTACA
    ACCACTGCCG CCACCACCAT GGAGAGGCCT CCCACCACCA CCTCCAAGAC TAAGATGCCC
    GAATCCGCCC CCGACAGGCA GTCCATATGG AACGTCACGG TGCTCCCCAA CAGTAAATGG
    GCCAACATCA CCTGGAGACA CAATCTCGGG CCCGGAACTG CCTTTGTGGT TGAGTACATC
    GACAGCAACC ATACGAAAAA AACTGTCCCT GTTAAGAGCC AGGTCCAGCC CGTACAGCTG
    ACAGACCTCT ATCCCGGGAT GACATACACG TTGCGCGTTT ATTCCCGGGA CAAGGAGGGC
    ATCAGCAGTA CCGTCATCAC CTTTATGACC AGTACAGCTT ACACCAATAA CCAGGCAGAC
    ATCGCCACCC AGGGCTGGTT CATTGGGCTC ATGTGTGCCA TCGCCCTCCT GGTGCTGATC
    CTGCTCATCG TCTGCTTCAT CAAGAGGAGT CGAGGTGGCA AATACCCAGT GCGAGAAAAG
    AAGGACGTTC CTCTTGGCCC CGAAGACCCC AAGGAAGAGG ATGGCTCCTT TGACTACAGT
    GACGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACGTCCC TCGATGGTAC CATCAAGCAG
    CAAGAGAGCG ACGACAGCCT GGTGGACTAT GGCGAGGGTG GCGAGGGGCA GTTCAATGAG
    GATGGGTCCT TCATCGGCCA GTACACGGTC AAAAAGGACA AAGAGGAGAC AGAGGGCAAT
    GAAAGCTCAG AGGCCACATC GCCTGTCAAC GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45317
    Clone ID Related Accession (Same CDS sequence) XM_005640780.3 , XM_005640780.2
    Accession Version XM_005640780.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3588bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurofascin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005640780.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TACTCCAACA GCAGCTTACA CCAATAACCA GGCAGACATC
    GCCACCCAGG GCTGGTTCAT TGGGCTCATG TGTGCCATCG CCCTCCTGGT GCTGATCCTG
    CTCATCGTCT GCTTCATCAA GAGGAGTCGA GGTGGCAAAT ACCCAGTGCG AGAAAAGAAG
    GACGTTCCTC TTGGCCCCGA AGACCCCAAG GAAGAGGATG GCTCCTTTGA CTACAGTGAC
    GAGGACAACA AGCCCCTGCA GGGCAGCCAG ACGTCCCTCG ATGGTACCAT CAAGCAGCAA
    GAGAGCGACG ACAGCCTGGT GGACTATGGC GAGGGTGGCG AGGGGCAGTT CAATGAGGAT
    GGGTCCTTCA TCGGCCAGTA CACGGTCAAA AAGGACAAAG AGGAGACAGA GGGCAATGAA
    AGCTCAGAGG CCACATCGCC TGTCAACGCC ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640837.2
    CDS303..3890
    Translation

    Target ORF information:

    RefSeq Version XM_005640780.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640780.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TACTCCAACA GCAGCTTACA CCAATAACCA GGCAGACATC
    GCCACCCAGG GCTGGTTCAT TGGGCTCATG TGTGCCATCG CCCTCCTGGT GCTGATCCTG
    CTCATCGTCT GCTTCATCAA GAGGAGTCGA GGTGGCAAAT ACCCAGTGCG AGAAAAGAAG
    GACGTTCCTC TTGGCCCCGA AGACCCCAAG GAAGAGGATG GCTCCTTTGA CTACAGTGAC
    GAGGACAACA AGCCCCTGCA GGGCAGCCAG ACGTCCCTCG ATGGTACCAT CAAGCAGCAA
    GAGAGCGACG ACAGCCTGGT GGACTATGGC GAGGGTGGCG AGGGGCAGTT CAATGAGGAT
    GGGTCCTTCA TCGGCCAGTA CACGGTCAAA AAGGACAAAG AGGAGACAGA GGGCAATGAA
    AGCTCAGAGG CCACATCGCC TGTCAACGCC ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45317
    Clone ID Related Accession (Same CDS sequence) XM_005640780.3 , XM_005640780.2
    Accession Version XM_005640780.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3588bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neurofascin isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640780.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TACTCCAACA GCAGCTTACA CCAATAACCA GGCAGACATC
    GCCACCCAGG GCTGGTTCAT TGGGCTCATG TGTGCCATCG CCCTCCTGGT GCTGATCCTG
    CTCATCGTCT GCTTCATCAA GAGGAGTCGA GGTGGCAAAT ACCCAGTGCG AGAAAAGAAG
    GACGTTCCTC TTGGCCCCGA AGACCCCAAG GAAGAGGATG GCTCCTTTGA CTACAGTGAC
    GAGGACAACA AGCCCCTGCA GGGCAGCCAG ACGTCCCTCG ATGGTACCAT CAAGCAGCAA
    GAGAGCGACG ACAGCCTGGT GGACTATGGC GAGGGTGGCG AGGGGCAGTT CAATGAGGAT
    GGGTCCTTCA TCGGCCAGTA CACGGTCAAA AAGGACAAAG AGGAGACAGA GGGCAATGAA
    AGCTCAGAGG CCACATCGCC TGTCAACGCC ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640837.2
    CDS287..3874
    Translation

    Target ORF information:

    RefSeq Version XM_005640780.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640780.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TACTCCAACA GCAGCTTACA CCAATAACCA GGCAGACATC
    GCCACCCAGG GCTGGTTCAT TGGGCTCATG TGTGCCATCG CCCTCCTGGT GCTGATCCTG
    CTCATCGTCT GCTTCATCAA GAGGAGTCGA GGTGGCAAAT ACCCAGTGCG AGAAAAGAAG
    GACGTTCCTC TTGGCCCCGA AGACCCCAAG GAAGAGGATG GCTCCTTTGA CTACAGTGAC
    GAGGACAACA AGCCCCTGCA GGGCAGCCAG ACGTCCCTCG ATGGTACCAT CAAGCAGCAA
    GAGAGCGACG ACAGCCTGGT GGACTATGGC GAGGGTGGCG AGGGGCAGTT CAATGAGGAT
    GGGTCCTTCA TCGGCCAGTA CACGGTCAAA AAGGACAAAG AGGAGACAGA GGGCAATGAA
    AGCTCAGAGG CCACATCGCC TGTCAACGCC ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45318
    Clone ID Related Accession (Same CDS sequence) XM_005640781.3 , XM_005640781.2
    Accession Version XM_005640781.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3573bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurofascin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005640781.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TTACACCAAT AACCAGGCAG ACATCGCCAC CCAGGGCTGG
    TTCATTGGGC TCATGTGTGC CATCGCCCTC CTGGTGCTGA TCCTGCTCAT CGTCTGCTTC
    ATCAAGAGGA GTCGAGGTGG CAAATACCCA GTGCGAGAAA AGAAGGACGT TCCTCTTGGC
    CCCGAAGACC CCAAGGAAGA GGATGGCTCC TTTGACTACA GTGACGAGGA CAACAAGCCC
    CTGCAGGGCA GCCAGACGTC CCTCGATGGT ACCATCAAGC AGCAAGAGAG CGACGACAGC
    CTGGTGGACT ATGGCGAGGG TGGCGAGGGG CAGTTCAATG AGGATGGGTC CTTCATCGGC
    CAGTACACGG TCAAAAAGGA CAAAGAGGAG ACAGAGGGCA ATGAAAGCTC AGAGGCCACA
    TCGCCTGTCA ACGCCATCTA TTCTCTGGCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640838.2
    CDS303..3875
    Translation

    Target ORF information:

    RefSeq Version XM_005640781.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640781.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TTACACCAAT AACCAGGCAG ACATCGCCAC CCAGGGCTGG
    TTCATTGGGC TCATGTGTGC CATCGCCCTC CTGGTGCTGA TCCTGCTCAT CGTCTGCTTC
    ATCAAGAGGA GTCGAGGTGG CAAATACCCA GTGCGAGAAA AGAAGGACGT TCCTCTTGGC
    CCCGAAGACC CCAAGGAAGA GGATGGCTCC TTTGACTACA GTGACGAGGA CAACAAGCCC
    CTGCAGGGCA GCCAGACGTC CCTCGATGGT ACCATCAAGC AGCAAGAGAG CGACGACAGC
    CTGGTGGACT ATGGCGAGGG TGGCGAGGGG CAGTTCAATG AGGATGGGTC CTTCATCGGC
    CAGTACACGG TCAAAAAGGA CAAAGAGGAG ACAGAGGGCA ATGAAAGCTC AGAGGCCACA
    TCGCCTGTCA ACGCCATCTA TTCTCTGGCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45318
    Clone ID Related Accession (Same CDS sequence) XM_005640781.3 , XM_005640781.2
    Accession Version XM_005640781.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3573bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neurofascin isoform X11
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640781.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TTACACCAAT AACCAGGCAG ACATCGCCAC CCAGGGCTGG
    TTCATTGGGC TCATGTGTGC CATCGCCCTC CTGGTGCTGA TCCTGCTCAT CGTCTGCTTC
    ATCAAGAGGA GTCGAGGTGG CAAATACCCA GTGCGAGAAA AGAAGGACGT TCCTCTTGGC
    CCCGAAGACC CCAAGGAAGA GGATGGCTCC TTTGACTACA GTGACGAGGA CAACAAGCCC
    CTGCAGGGCA GCCAGACGTC CCTCGATGGT ACCATCAAGC AGCAAGAGAG CGACGACAGC
    CTGGTGGACT ATGGCGAGGG TGGCGAGGGG CAGTTCAATG AGGATGGGTC CTTCATCGGC
    CAGTACACGG TCAAAAAGGA CAAAGAGGAG ACAGAGGGCA ATGAAAGCTC AGAGGCCACA
    TCGCCTGTCA ACGCCATCTA TTCTCTGGCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640838.2
    CDS287..3859
    Translation

    Target ORF information:

    RefSeq Version XM_005640781.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640781.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TTACACCAAT AACCAGGCAG ACATCGCCAC CCAGGGCTGG
    TTCATTGGGC TCATGTGTGC CATCGCCCTC CTGGTGCTGA TCCTGCTCAT CGTCTGCTTC
    ATCAAGAGGA GTCGAGGTGG CAAATACCCA GTGCGAGAAA AGAAGGACGT TCCTCTTGGC
    CCCGAAGACC CCAAGGAAGA GGATGGCTCC TTTGACTACA GTGACGAGGA CAACAAGCCC
    CTGCAGGGCA GCCAGACGTC CCTCGATGGT ACCATCAAGC AGCAAGAGAG CGACGACAGC
    CTGGTGGACT ATGGCGAGGG TGGCGAGGGG CAGTTCAATG AGGATGGGTC CTTCATCGGC
    CAGTACACGG TCAAAAAGGA CAAAGAGGAG ACAGAGGGCA ATGAAAGCTC AGAGGCCACA
    TCGCCTGTCA ACGCCATCTA TTCTCTGGCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45319
    Clone ID Related Accession (Same CDS sequence) XM_545682.5 , XM_545682.6
    Accession Version XM_545682.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3570bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neurofascin isoform X12
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_545682.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CTATCCCAGC CCCCAACCAT CACCAAGCAG
    TCAGTGAAGG ACCACATCGT GGATCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAG
    GGGAACCCTG CCCCCAGCTT CCACTGGACT CGAAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCAATGAG GAGAAAGTCT GGGACCCTGG TGATCGACTT CCGTAGTGGT
    GGGCGGCCCG AAGAATATGA AGGGGAATAC CAGTGCTTTG CCCGCAACAA ATTTGGCACG
    GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAGTCTC CCCTGTGGCC CAAGGAGAAC
    CTAGACCCAG TCGTGGTTCA AGAAGGTGCT CCTTTGACAC TCCAGTGCAA CCCTCCACCC
    GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACTCAGGAC
    AAGCGTGTCT CTCAGGGCCA CAACGGTGAC CTGTATTTCT CCAACGTGAT GCTTCAGGAT
    ATGCAGACAG ACTACAGCTG CAACGCGCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC
    CACCCTGACA TGTACAGTGC ACGAGGAGTT GCAGAAAGGA CACCCAGCTT CATGTATCCC
    CAGGGCACAG CGAGCAGTCA GATGGTGCTG CGTGGCACGG ATCTTCTGCT GGAGTGCATC
    GCCTCTGGGG TCCCCACGCC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT
    GACAAGGCCA AGTTTGAGAA CTTTAACAAG GCTCTGCGTA TCACCAACGT CTCCGAGGAA
    GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC
    TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATCCTGGCT
    CCCGGTGAGG ACGGGAGACT GGTGTGTCGA GCCAATGGGA ACCCCAAGCC CACTGTCCAG
    TGGATGGTAA ATGGGGAACC ACTGCAATCG GCACCACCCA ACCCAAACCG TGAGGTGGCT
    GGGGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GAGCTGTGTA CCAGTGCAAC
    ACCTCCAACG AGCATGGCTA CCTACTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCA
    CCCCGGATGC TGTCACCCCG GAACCAGCTC ATTAGGGTGA TCCTGTACAA CCGGACACGG
    CTGGACTGCC CATTCTTCGG GTCTCCCATC CCCACACTCC GATGGTTTAA GAACGGGCAG
    GGAAGTAACC TGGATGGTGG TAACTACCAC GTCTACGAGA ATGGCAGTCT GGAAATCAAG
    ATGATCCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA
    GCTGAGAACC AGGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCTGAA
    GACCAGGTGG CCAAAAGGGG CACGACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC
    TCCCTGAAGC TCACGGTCTC CTGGCTGAAG GATGATGAGC CTCTCTACAT AGGAAACAGG
    ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGTGTGG CTGAGCGGGA CCAGGGCAGT
    TACACATGTG TTGCCAGCAC TGAGCTGGAC CAGGACCTGG CCAAGGCCTA CCTCACCGTG
    CTAGCTGATC AGGCCACTCC AACTAACCGT TTGGCTGCCC TGCCCAAAGG ACGGCCAGAC
    CGACCCCGGG ACTTGGAGCT CACTGACCTG GCTGAGAGGA GTGTGAGGCT GACCTGGATC
    CCAGGGGATG ATAACAACAG CCCCATCACA GACTACGTCG TCCAGTTTGA AGAGGACCAG
    TTCCAGCCAG GGGTCTGGCA TGACCATTCC AAGTTCCCAG GCAGCGTCAA CTCTGCCATC
    CTCCAGTTGT CCCCATATGT CAACTACCAG TTCCGGGTCA TCGCCATCAA CGAGGTGGGC
    AGCAGCCATC CCAGCCTCCC CTCGGAGCGC TACAGGACCA GCGGGGCGCC CCCTGAGTCC
    AATCCCGCCG ACGTAAAGGG AGAAGGGACC AGAAAGAACA ACATGGAGAT CACTTGGACG
    CCCATGAACG CCACCTCTGC CTTTGGCCCC AACCTGCGCT ACATCGTCAA GTGGCGGCGG
    AGAGAAACTC GAGAGACGTG GAACAACGCC ACAGTGTGGG GCTCCCGCTA CGTGGTGGGC
    CAGACCCCTG TCTACGTGCC CTATGAGATC CGAGTCCAGG CCGAAAATGA CTTTGGGAAG
    GGCCCTGAGC CAGACACTGT CATCGGTTAC TCCGGGGAAG ACTATCCCAG GGCTGCACCC
    ACTGACGTTA AAATCCGAGT CCTGAACAGC ACAGCCATCA GTCTCCAGTG GAACCGCGTT
    TACCCTGACA CAGTCCAGGG CCAGCTCAGA GAGTACCGAG CCTACTACTG GAGGGAGAGC
    AGCTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AAGCCAGCTT CCCTGGTGAC
    CGCCCCCGCG GCGTGGTGTC CCGCCTCTTC CCCTACAGTA ACTACAAGCT GGAGATGGTC
    GTGGTCAATG GGAGAGGCGA TGGGCCTCGC AGTGAAACCA AGGAGTTCAC CACCCCGGAA
    GGAGTACCCA GTGCCCCCAG ACGTTTCCGA GTCCGGCAGC CCAACCTGGA GACAATCAAC
    CTGGAATGGG ATCATCCGGA ACACCCCAAT GGGATCCTGA TCGGATACAC TCTCAAATAT
    GTGGCTTTTA ATGGAACCAA ATTAGGAAAG CAGATAGTGG AAAACTTCTC TCCCAATCAG
    ACCAAGTTCA CGATGCAAAG AGCAGACCCC GTGTCGCGCT ACCGCTTTAC CCTCAGTGCC
    AGGACGCAGG TGGGCTCTGG GGAAGCAGTC ACAGAGGAGT CACCAGCACC CCCAAATGAA
    GCTACTCCAA CAGCAGCTTA CACCAATAAC CAGGCAGACA TCGCCACCCA GGGCTGGTTC
    ATTGGGCTCA TGTGTGCCAT CGCCCTCCTG GTGCTGATCC TGCTCATCGT CTGCTTCATC
    AAGAGGAGTC GAGGTGGCAA ATACCCAGTG CGAGAAAAGA AGGACGTTCC TCTTGGCCCC
    GAAGACCCCA AGGAAGAGGA TGGCTCCTTT GACTACAGTG ACGAGGACAA CAAGCCCCTG
    CAGGGCAGCC AGACGTCCCT CGATGGTACC ATCAAGCAGC AAGAGAGCGA CGACAGCCTG
    GTGGACTATG GCGAGGGTGG CGAGGGGCAG TTCAATGAGG ATGGGTCCTT CATCGGCCAG
    TACACGGTCA AAAAGGACAA AGAGGAGACA GAGGGCAATG AAAGCTCAGA GGCCACATCG
    CCTGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_545682.5
    CDS287..3856
    Translation

    Target ORF information:

    RefSeq Version XM_545682.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_545682.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CTATCCCAGC CCCCAACCAT CACCAAGCAG
    TCAGTGAAGG ACCACATCGT GGATCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAG
    GGGAACCCTG CCCCCAGCTT CCACTGGACT CGAAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCAATGAG GAGAAAGTCT GGGACCCTGG TGATCGACTT CCGTAGTGGT
    GGGCGGCCCG AAGAATATGA AGGGGAATAC CAGTGCTTTG CCCGCAACAA ATTTGGCACG
    GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAGTCTC CCCTGTGGCC CAAGGAGAAC
    CTAGACCCAG TCGTGGTTCA AGAAGGTGCT CCTTTGACAC TCCAGTGCAA CCCTCCACCC
    GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACTCAGGAC
    AAGCGTGTCT CTCAGGGCCA CAACGGTGAC CTGTATTTCT CCAACGTGAT GCTTCAGGAT
    ATGCAGACAG ACTACAGCTG CAACGCGCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC
    CACCCTGACA TGTACAGTGC ACGAGGAGTT GCAGAAAGGA CACCCAGCTT CATGTATCCC
    CAGGGCACAG CGAGCAGTCA GATGGTGCTG CGTGGCACGG ATCTTCTGCT GGAGTGCATC
    GCCTCTGGGG TCCCCACGCC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT
    GACAAGGCCA AGTTTGAGAA CTTTAACAAG GCTCTGCGTA TCACCAACGT CTCCGAGGAA
    GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC
    TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATCCTGGCT
    CCCGGTGAGG ACGGGAGACT GGTGTGTCGA GCCAATGGGA ACCCCAAGCC CACTGTCCAG
    TGGATGGTAA ATGGGGAACC ACTGCAATCG GCACCACCCA ACCCAAACCG TGAGGTGGCT
    GGGGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GAGCTGTGTA CCAGTGCAAC
    ACCTCCAACG AGCATGGCTA CCTACTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCA
    CCCCGGATGC TGTCACCCCG GAACCAGCTC ATTAGGGTGA TCCTGTACAA CCGGACACGG
    CTGGACTGCC CATTCTTCGG GTCTCCCATC CCCACACTCC GATGGTTTAA GAACGGGCAG
    GGAAGTAACC TGGATGGTGG TAACTACCAC GTCTACGAGA ATGGCAGTCT GGAAATCAAG
    ATGATCCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA
    GCTGAGAACC AGGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCTGAA
    GACCAGGTGG CCAAAAGGGG CACGACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC
    TCCCTGAAGC TCACGGTCTC CTGGCTGAAG GATGATGAGC CTCTCTACAT AGGAAACAGG
    ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGTGTGG CTGAGCGGGA CCAGGGCAGT
    TACACATGTG TTGCCAGCAC TGAGCTGGAC CAGGACCTGG CCAAGGCCTA CCTCACCGTG
    CTAGCTGATC AGGCCACTCC AACTAACCGT TTGGCTGCCC TGCCCAAAGG ACGGCCAGAC
    CGACCCCGGG ACTTGGAGCT CACTGACCTG GCTGAGAGGA GTGTGAGGCT GACCTGGATC
    CCAGGGGATG ATAACAACAG CCCCATCACA GACTACGTCG TCCAGTTTGA AGAGGACCAG
    TTCCAGCCAG GGGTCTGGCA TGACCATTCC AAGTTCCCAG GCAGCGTCAA CTCTGCCATC
    CTCCAGTTGT CCCCATATGT CAACTACCAG TTCCGGGTCA TCGCCATCAA CGAGGTGGGC
    AGCAGCCATC CCAGCCTCCC CTCGGAGCGC TACAGGACCA GCGGGGCGCC CCCTGAGTCC
    AATCCCGCCG ACGTAAAGGG AGAAGGGACC AGAAAGAACA ACATGGAGAT CACTTGGACG
    CCCATGAACG CCACCTCTGC CTTTGGCCCC AACCTGCGCT ACATCGTCAA GTGGCGGCGG
    AGAGAAACTC GAGAGACGTG GAACAACGCC ACAGTGTGGG GCTCCCGCTA CGTGGTGGGC
    CAGACCCCTG TCTACGTGCC CTATGAGATC CGAGTCCAGG CCGAAAATGA CTTTGGGAAG
    GGCCCTGAGC CAGACACTGT CATCGGTTAC TCCGGGGAAG ACTATCCCAG GGCTGCACCC
    ACTGACGTTA AAATCCGAGT CCTGAACAGC ACAGCCATCA GTCTCCAGTG GAACCGCGTT
    TACCCTGACA CAGTCCAGGG CCAGCTCAGA GAGTACCGAG CCTACTACTG GAGGGAGAGC
    AGCTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AAGCCAGCTT CCCTGGTGAC
    CGCCCCCGCG GCGTGGTGTC CCGCCTCTTC CCCTACAGTA ACTACAAGCT GGAGATGGTC
    GTGGTCAATG GGAGAGGCGA TGGGCCTCGC AGTGAAACCA AGGAGTTCAC CACCCCGGAA
    GGAGTACCCA GTGCCCCCAG ACGTTTCCGA GTCCGGCAGC CCAACCTGGA GACAATCAAC
    CTGGAATGGG ATCATCCGGA ACACCCCAAT GGGATCCTGA TCGGATACAC TCTCAAATAT
    GTGGCTTTTA ATGGAACCAA ATTAGGAAAG CAGATAGTGG AAAACTTCTC TCCCAATCAG
    ACCAAGTTCA CGATGCAAAG AGCAGACCCC GTGTCGCGCT ACCGCTTTAC CCTCAGTGCC
    AGGACGCAGG TGGGCTCTGG GGAAGCAGTC ACAGAGGAGT CACCAGCACC CCCAAATGAA
    GCTACTCCAA CAGCAGCTTA CACCAATAAC CAGGCAGACA TCGCCACCCA GGGCTGGTTC
    ATTGGGCTCA TGTGTGCCAT CGCCCTCCTG GTGCTGATCC TGCTCATCGT CTGCTTCATC
    AAGAGGAGTC GAGGTGGCAA ATACCCAGTG CGAGAAAAGA AGGACGTTCC TCTTGGCCCC
    GAAGACCCCA AGGAAGAGGA TGGCTCCTTT GACTACAGTG ACGAGGACAA CAAGCCCCTG
    CAGGGCAGCC AGACGTCCCT CGATGGTACC ATCAAGCAGC AAGAGAGCGA CGACAGCCTG
    GTGGACTATG GCGAGGGTGG CGAGGGGCAG TTCAATGAGG ATGGGTCCTT CATCGGCCAG
    TACACGGTCA AAAAGGACAA AGAGGAGACA GAGGGCAATG AAAGCTCAGA GGCCACATCG
    CCTGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45319
    Clone ID Related Accession (Same CDS sequence) XM_545682.5 , XM_545682.6
    Accession Version XM_545682.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3570bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurofascin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_545682.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CTATCCCAGC CCCCAACCAT CACCAAGCAG
    TCAGTGAAGG ACCACATCGT GGATCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAG
    GGGAACCCTG CCCCCAGCTT CCACTGGACT CGAAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCAATGAG GAGAAAGTCT GGGACCCTGG TGATCGACTT CCGTAGTGGT
    GGGCGGCCCG AAGAATATGA AGGGGAATAC CAGTGCTTTG CCCGCAACAA ATTTGGCACG
    GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAGTCTC CCCTGTGGCC CAAGGAGAAC
    CTAGACCCAG TCGTGGTTCA AGAAGGTGCT CCTTTGACAC TCCAGTGCAA CCCTCCACCC
    GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACTCAGGAC
    AAGCGTGTCT CTCAGGGCCA CAACGGTGAC CTGTATTTCT CCAACGTGAT GCTTCAGGAT
    ATGCAGACAG ACTACAGCTG CAACGCGCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC
    CACCCTGACA TGTACAGTGC ACGAGGAGTT GCAGAAAGGA CACCCAGCTT CATGTATCCC
    CAGGGCACAG CGAGCAGTCA GATGGTGCTG CGTGGCACGG ATCTTCTGCT GGAGTGCATC
    GCCTCTGGGG TCCCCACGCC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT
    GACAAGGCCA AGTTTGAGAA CTTTAACAAG GCTCTGCGTA TCACCAACGT CTCCGAGGAA
    GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC
    TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATCCTGGCT
    CCCGGTGAGG ACGGGAGACT GGTGTGTCGA GCCAATGGGA ACCCCAAGCC CACTGTCCAG
    TGGATGGTAA ATGGGGAACC ACTGCAATCG GCACCACCCA ACCCAAACCG TGAGGTGGCT
    GGGGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GAGCTGTGTA CCAGTGCAAC
    ACCTCCAACG AGCATGGCTA CCTACTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCA
    CCCCGGATGC TGTCACCCCG GAACCAGCTC ATTAGGGTGA TCCTGTACAA CCGGACACGG
    CTGGACTGCC CATTCTTCGG GTCTCCCATC CCCACACTCC GATGGTTTAA GAACGGGCAG
    GGAAGTAACC TGGATGGTGG TAACTACCAC GTCTACGAGA ATGGCAGTCT GGAAATCAAG
    ATGATCCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA
    GCTGAGAACC AGGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCTGAA
    GACCAGGTGG CCAAAAGGGG CACGACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC
    TCCCTGAAGC TCACGGTCTC CTGGCTGAAG GATGATGAGC CTCTCTACAT AGGAAACAGG
    ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGTGTGG CTGAGCGGGA CCAGGGCAGT
    TACACATGTG TTGCCAGCAC TGAGCTGGAC CAGGACCTGG CCAAGGCCTA CCTCACCGTG
    CTAGCTGATC AGGCCACTCC AACTAACCGT TTGGCTGCCC TGCCCAAAGG ACGGCCAGAC
    CGACCCCGGG ACTTGGAGCT CACTGACCTG GCTGAGAGGA GTGTGAGGCT GACCTGGATC
    CCAGGGGATG ATAACAACAG CCCCATCACA GACTACGTCG TCCAGTTTGA AGAGGACCAG
    TTCCAGCCAG GGGTCTGGCA TGACCATTCC AAGTTCCCAG GCAGCGTCAA CTCTGCCATC
    CTCCAGTTGT CCCCATATGT CAACTACCAG TTCCGGGTCA TCGCCATCAA CGAGGTGGGC
    AGCAGCCATC CCAGCCTCCC CTCGGAGCGC TACAGGACCA GCGGGGCGCC CCCTGAGTCC
    AATCCCGCCG ACGTAAAGGG AGAAGGGACC AGAAAGAACA ACATGGAGAT CACTTGGACG
    CCCATGAACG CCACCTCTGC CTTTGGCCCC AACCTGCGCT ACATCGTCAA GTGGCGGCGG
    AGAGAAACTC GAGAGACGTG GAACAACGCC ACAGTGTGGG GCTCCCGCTA CGTGGTGGGC
    CAGACCCCTG TCTACGTGCC CTATGAGATC CGAGTCCAGG CCGAAAATGA CTTTGGGAAG
    GGCCCTGAGC CAGACACTGT CATCGGTTAC TCCGGGGAAG ACTATCCCAG GGCTGCACCC
    ACTGACGTTA AAATCCGAGT CCTGAACAGC ACAGCCATCA GTCTCCAGTG GAACCGCGTT
    TACCCTGACA CAGTCCAGGG CCAGCTCAGA GAGTACCGAG CCTACTACTG GAGGGAGAGC
    AGCTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AAGCCAGCTT CCCTGGTGAC
    CGCCCCCGCG GCGTGGTGTC CCGCCTCTTC CCCTACAGTA ACTACAAGCT GGAGATGGTC
    GTGGTCAATG GGAGAGGCGA TGGGCCTCGC AGTGAAACCA AGGAGTTCAC CACCCCGGAA
    GGAGTACCCA GTGCCCCCAG ACGTTTCCGA GTCCGGCAGC CCAACCTGGA GACAATCAAC
    CTGGAATGGG ATCATCCGGA ACACCCCAAT GGGATCCTGA TCGGATACAC TCTCAAATAT
    GTGGCTTTTA ATGGAACCAA ATTAGGAAAG CAGATAGTGG AAAACTTCTC TCCCAATCAG
    ACCAAGTTCA CGATGCAAAG AGCAGACCCC GTGTCGCGCT ACCGCTTTAC CCTCAGTGCC
    AGGACGCAGG TGGGCTCTGG GGAAGCAGTC ACAGAGGAGT CACCAGCACC CCCAAATGAA
    GCTACTCCAA CAGCAGCTTA CACCAATAAC CAGGCAGACA TCGCCACCCA GGGCTGGTTC
    ATTGGGCTCA TGTGTGCCAT CGCCCTCCTG GTGCTGATCC TGCTCATCGT CTGCTTCATC
    AAGAGGAGTC GAGGTGGCAA ATACCCAGTG CGAGAAAAGA AGGACGTTCC TCTTGGCCCC
    GAAGACCCCA AGGAAGAGGA TGGCTCCTTT GACTACAGTG ACGAGGACAA CAAGCCCCTG
    CAGGGCAGCC AGACGTCCCT CGATGGTACC ATCAAGCAGC AAGAGAGCGA CGACAGCCTG
    GTGGACTATG GCGAGGGTGG CGAGGGGCAG TTCAATGAGG ATGGGTCCTT CATCGGCCAG
    TACACGGTCA AAAAGGACAA AGAGGAGACA GAGGGCAATG AAAGCTCAGA GGCCACATCG
    CCTGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_545682.5
    CDS303..3872
    Translation

    Target ORF information:

    RefSeq Version XM_545682.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_545682.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CTATCCCAGC CCCCAACCAT CACCAAGCAG
    TCAGTGAAGG ACCACATCGT GGATCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAG
    GGGAACCCTG CCCCCAGCTT CCACTGGACT CGAAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCAATGAG GAGAAAGTCT GGGACCCTGG TGATCGACTT CCGTAGTGGT
    GGGCGGCCCG AAGAATATGA AGGGGAATAC CAGTGCTTTG CCCGCAACAA ATTTGGCACG
    GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAGTCTC CCCTGTGGCC CAAGGAGAAC
    CTAGACCCAG TCGTGGTTCA AGAAGGTGCT CCTTTGACAC TCCAGTGCAA CCCTCCACCC
    GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACTCAGGAC
    AAGCGTGTCT CTCAGGGCCA CAACGGTGAC CTGTATTTCT CCAACGTGAT GCTTCAGGAT
    ATGCAGACAG ACTACAGCTG CAACGCGCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC
    CACCCTGACA TGTACAGTGC ACGAGGAGTT GCAGAAAGGA CACCCAGCTT CATGTATCCC
    CAGGGCACAG CGAGCAGTCA GATGGTGCTG CGTGGCACGG ATCTTCTGCT GGAGTGCATC
    GCCTCTGGGG TCCCCACGCC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT
    GACAAGGCCA AGTTTGAGAA CTTTAACAAG GCTCTGCGTA TCACCAACGT CTCCGAGGAA
    GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC
    TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATCCTGGCT
    CCCGGTGAGG ACGGGAGACT GGTGTGTCGA GCCAATGGGA ACCCCAAGCC CACTGTCCAG
    TGGATGGTAA ATGGGGAACC ACTGCAATCG GCACCACCCA ACCCAAACCG TGAGGTGGCT
    GGGGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GAGCTGTGTA CCAGTGCAAC
    ACCTCCAACG AGCATGGCTA CCTACTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCA
    CCCCGGATGC TGTCACCCCG GAACCAGCTC ATTAGGGTGA TCCTGTACAA CCGGACACGG
    CTGGACTGCC CATTCTTCGG GTCTCCCATC CCCACACTCC GATGGTTTAA GAACGGGCAG
    GGAAGTAACC TGGATGGTGG TAACTACCAC GTCTACGAGA ATGGCAGTCT GGAAATCAAG
    ATGATCCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA
    GCTGAGAACC AGGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCTGAA
    GACCAGGTGG CCAAAAGGGG CACGACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC
    TCCCTGAAGC TCACGGTCTC CTGGCTGAAG GATGATGAGC CTCTCTACAT AGGAAACAGG
    ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGTGTGG CTGAGCGGGA CCAGGGCAGT
    TACACATGTG TTGCCAGCAC TGAGCTGGAC CAGGACCTGG CCAAGGCCTA CCTCACCGTG
    CTAGCTGATC AGGCCACTCC AACTAACCGT TTGGCTGCCC TGCCCAAAGG ACGGCCAGAC
    CGACCCCGGG ACTTGGAGCT CACTGACCTG GCTGAGAGGA GTGTGAGGCT GACCTGGATC
    CCAGGGGATG ATAACAACAG CCCCATCACA GACTACGTCG TCCAGTTTGA AGAGGACCAG
    TTCCAGCCAG GGGTCTGGCA TGACCATTCC AAGTTCCCAG GCAGCGTCAA CTCTGCCATC
    CTCCAGTTGT CCCCATATGT CAACTACCAG TTCCGGGTCA TCGCCATCAA CGAGGTGGGC
    AGCAGCCATC CCAGCCTCCC CTCGGAGCGC TACAGGACCA GCGGGGCGCC CCCTGAGTCC
    AATCCCGCCG ACGTAAAGGG AGAAGGGACC AGAAAGAACA ACATGGAGAT CACTTGGACG
    CCCATGAACG CCACCTCTGC CTTTGGCCCC AACCTGCGCT ACATCGTCAA GTGGCGGCGG
    AGAGAAACTC GAGAGACGTG GAACAACGCC ACAGTGTGGG GCTCCCGCTA CGTGGTGGGC
    CAGACCCCTG TCTACGTGCC CTATGAGATC CGAGTCCAGG CCGAAAATGA CTTTGGGAAG
    GGCCCTGAGC CAGACACTGT CATCGGTTAC TCCGGGGAAG ACTATCCCAG GGCTGCACCC
    ACTGACGTTA AAATCCGAGT CCTGAACAGC ACAGCCATCA GTCTCCAGTG GAACCGCGTT
    TACCCTGACA CAGTCCAGGG CCAGCTCAGA GAGTACCGAG CCTACTACTG GAGGGAGAGC
    AGCTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AAGCCAGCTT CCCTGGTGAC
    CGCCCCCGCG GCGTGGTGTC CCGCCTCTTC CCCTACAGTA ACTACAAGCT GGAGATGGTC
    GTGGTCAATG GGAGAGGCGA TGGGCCTCGC AGTGAAACCA AGGAGTTCAC CACCCCGGAA
    GGAGTACCCA GTGCCCCCAG ACGTTTCCGA GTCCGGCAGC CCAACCTGGA GACAATCAAC
    CTGGAATGGG ATCATCCGGA ACACCCCAAT GGGATCCTGA TCGGATACAC TCTCAAATAT
    GTGGCTTTTA ATGGAACCAA ATTAGGAAAG CAGATAGTGG AAAACTTCTC TCCCAATCAG
    ACCAAGTTCA CGATGCAAAG AGCAGACCCC GTGTCGCGCT ACCGCTTTAC CCTCAGTGCC
    AGGACGCAGG TGGGCTCTGG GGAAGCAGTC ACAGAGGAGT CACCAGCACC CCCAAATGAA
    GCTACTCCAA CAGCAGCTTA CACCAATAAC CAGGCAGACA TCGCCACCCA GGGCTGGTTC
    ATTGGGCTCA TGTGTGCCAT CGCCCTCCTG GTGCTGATCC TGCTCATCGT CTGCTTCATC
    AAGAGGAGTC GAGGTGGCAA ATACCCAGTG CGAGAAAAGA AGGACGTTCC TCTTGGCCCC
    GAAGACCCCA AGGAAGAGGA TGGCTCCTTT GACTACAGTG ACGAGGACAA CAAGCCCCTG
    CAGGGCAGCC AGACGTCCCT CGATGGTACC ATCAAGCAGC AAGAGAGCGA CGACAGCCTG
    GTGGACTATG GCGAGGGTGG CGAGGGGCAG TTCAATGAGG ATGGGTCCTT CATCGGCCAG
    TACACGGTCA AAAAGGACAA AGAGGAGACA GAGGGCAATG AAAGCTCAGA GGCCACATCG
    CCTGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45320
    Clone ID Related Accession (Same CDS sequence) XM_850983.4 , XM_850983.5
    Accession Version XM_850983.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neurofascin isoform X13
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_850983.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CTATCCCAGC CCCCAACCAT CACCAAGCAG
    TCAGTGAAGG ACCACATCGT GGATCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAG
    GGGAACCCTG CCCCCAGCTT CCACTGGACT CGAAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCAATGAG GAGAAAGTCT GGGACCCTGG TGATCGACTT CCGTAGTGGT
    GGGCGGCCCG AAGAATATGA AGGGGAATAC CAGTGCTTTG CCCGCAACAA ATTTGGCACG
    GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAGTCTC CCCTGTGGCC CAAGGAGAAC
    CTAGACCCAG TCGTGGTTCA AGAAGGTGCT CCTTTGACAC TCCAGTGCAA CCCTCCACCC
    GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACTCAGGAC
    AAGCGTGTCT CTCAGGGCCA CAACGGTGAC CTGTATTTCT CCAACGTGAT GCTTCAGGAT
    ATGCAGACAG ACTACAGCTG CAACGCGCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC
    CACCCTGACA TGTACAGTGC ACGAGGAGTT GCAGAAAGGA CACCCAGCTT CATGTATCCC
    CAGGGCACAG CGAGCAGTCA GATGGTGCTG CGTGGCACGG ATCTTCTGCT GGAGTGCATC
    GCCTCTGGGG TCCCCACGCC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT
    GACAAGGCCA AGTTTGAGAA CTTTAACAAG GCTCTGCGTA TCACCAACGT CTCCGAGGAA
    GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC
    TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATCCTGGCT
    CCCGGTGAGG ACGGGAGACT GGTGTGTCGA GCCAATGGGA ACCCCAAGCC CACTGTCCAG
    TGGATGGTAA ATGGGGAACC ACTGCAATCG GCACCACCCA ACCCAAACCG TGAGGTGGCT
    GGGGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GAGCTGTGTA CCAGTGCAAC
    ACCTCCAACG AGCATGGCTA CCTACTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCA
    CCCCGGATGC TGTCACCCCG GAACCAGCTC ATTAGGGTGA TCCTGTACAA CCGGACACGG
    CTGGACTGCC CATTCTTCGG GTCTCCCATC CCCACACTCC GATGGTTTAA GAACGGGCAG
    GGAAGTAACC TGGATGGTGG TAACTACCAC GTCTACGAGA ATGGCAGTCT GGAAATCAAG
    ATGATCCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA
    GCTGAGAACC AGGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCTGAA
    GACCAGGTGG CCAAAAGGGG CACGACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC
    TCCCTGAAGC TCACGGTCTC CTGGCTGAAG GATGATGAGC CTCTCTACAT AGGAAACAGG
    ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGTGTGG CTGAGCGGGA CCAGGGCAGT
    TACACATGTG TTGCCAGCAC TGAGCTGGAC CAGGACCTGG CCAAGGCCTA CCTCACCGTG
    CTAGGACGGC CAGACCGACC CCGGGACTTG GAGCTCACTG ACCTGGCTGA GAGGAGTGTG
    AGGCTGACCT GGATCCCAGG GGATGATAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAGG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCAGGCAGC
    GTCAACTCTG CCATCCTCCA GTTGTCCCCA TATGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAACGAGG TGGGCAGCAG CCATCCCAGC CTCCCCTCGG AGCGCTACAG GACCAGCGGG
    GCGCCCCCTG AGTCCAATCC CGCCGACGTA AAGGGAGAAG GGACCAGAAA GAACAACATG
    GAGATCACTT GGACGCCCAT GAACGCCACC TCTGCCTTTG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA AACTCGAGAG ACGTGGAACA ACGCCACAGT GTGGGGCTCC
    CGCTACGTGG TGGGCCAGAC CCCTGTCTAC GTGCCCTATG AGATCCGAGT CCAGGCCGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCCGG GGAAGACTAT
    CCCAGGGCTG CACCCACTGA CGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGTCTC
    CAGTGGAACC GCGTTTACCC TGACACAGTC CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC
    AGCTTCCCTG GTGACCGCCC CCGCGGCGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTCGTGGT CAATGGGAGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGACGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCGGAACACC CCAATGGGAT CCTGATCGGA
    TACACTCTCA AATATGTGGC TTTTAATGGA ACCAAATTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGATG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTACCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGGGAAG CAGTCACAGA GGAGTCACCA
    GCACCCCCAA ATGAAGCTAC TCCAACAGCA GCTTACACCA ATAACCAGGC AGACATCGCC
    ACCCAGGGCT GGTTCATTGG GCTCATGTGT GCCATCGCCC TCCTGGTGCT GATCCTGCTC
    ATCGTCTGCT TCATCAAGAG GAGTCGAGGT GGCAAATACC CAGTGCGAGA AAAGAAGGAC
    GTTCCTCTTG GCCCCGAAGA CCCCAAGGAA GAGGATGGCT CCTTTGACTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTCGATG GTACCATCAA GCAGCAAGAG
    AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GGCAGTTCAA TGAGGATGGG
    TCCTTCATCG GCCAGTACAC GGTCAAAAAG GACAAAGAGG AGACAGAGGG CAATGAAAGC
    TCAGAGGCCA CATCGCCTGT CAACGCCATC TATTCTCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_856076.4
    CDS287..3811
    Translation

    Target ORF information:

    RefSeq Version XM_850983.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_850983.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CTATCCCAGC CCCCAACCAT CACCAAGCAG
    TCAGTGAAGG ACCACATCGT GGATCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAG
    GGGAACCCTG CCCCCAGCTT CCACTGGACT CGAAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCAATGAG GAGAAAGTCT GGGACCCTGG TGATCGACTT CCGTAGTGGT
    GGGCGGCCCG AAGAATATGA AGGGGAATAC CAGTGCTTTG CCCGCAACAA ATTTGGCACG
    GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAGTCTC CCCTGTGGCC CAAGGAGAAC
    CTAGACCCAG TCGTGGTTCA AGAAGGTGCT CCTTTGACAC TCCAGTGCAA CCCTCCACCC
    GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACTCAGGAC
    AAGCGTGTCT CTCAGGGCCA CAACGGTGAC CTGTATTTCT CCAACGTGAT GCTTCAGGAT
    ATGCAGACAG ACTACAGCTG CAACGCGCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC
    CACCCTGACA TGTACAGTGC ACGAGGAGTT GCAGAAAGGA CACCCAGCTT CATGTATCCC
    CAGGGCACAG CGAGCAGTCA GATGGTGCTG CGTGGCACGG ATCTTCTGCT GGAGTGCATC
    GCCTCTGGGG TCCCCACGCC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT
    GACAAGGCCA AGTTTGAGAA CTTTAACAAG GCTCTGCGTA TCACCAACGT CTCCGAGGAA
    GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC
    TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATCCTGGCT
    CCCGGTGAGG ACGGGAGACT GGTGTGTCGA GCCAATGGGA ACCCCAAGCC CACTGTCCAG
    TGGATGGTAA ATGGGGAACC ACTGCAATCG GCACCACCCA ACCCAAACCG TGAGGTGGCT
    GGGGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GAGCTGTGTA CCAGTGCAAC
    ACCTCCAACG AGCATGGCTA CCTACTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCA
    CCCCGGATGC TGTCACCCCG GAACCAGCTC ATTAGGGTGA TCCTGTACAA CCGGACACGG
    CTGGACTGCC CATTCTTCGG GTCTCCCATC CCCACACTCC GATGGTTTAA GAACGGGCAG
    GGAAGTAACC TGGATGGTGG TAACTACCAC GTCTACGAGA ATGGCAGTCT GGAAATCAAG
    ATGATCCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA
    GCTGAGAACC AGGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCTGAA
    GACCAGGTGG CCAAAAGGGG CACGACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC
    TCCCTGAAGC TCACGGTCTC CTGGCTGAAG GATGATGAGC CTCTCTACAT AGGAAACAGG
    ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGTGTGG CTGAGCGGGA CCAGGGCAGT
    TACACATGTG TTGCCAGCAC TGAGCTGGAC CAGGACCTGG CCAAGGCCTA CCTCACCGTG
    CTAGGACGGC CAGACCGACC CCGGGACTTG GAGCTCACTG ACCTGGCTGA GAGGAGTGTG
    AGGCTGACCT GGATCCCAGG GGATGATAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAGG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCAGGCAGC
    GTCAACTCTG CCATCCTCCA GTTGTCCCCA TATGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAACGAGG TGGGCAGCAG CCATCCCAGC CTCCCCTCGG AGCGCTACAG GACCAGCGGG
    GCGCCCCCTG AGTCCAATCC CGCCGACGTA AAGGGAGAAG GGACCAGAAA GAACAACATG
    GAGATCACTT GGACGCCCAT GAACGCCACC TCTGCCTTTG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA AACTCGAGAG ACGTGGAACA ACGCCACAGT GTGGGGCTCC
    CGCTACGTGG TGGGCCAGAC CCCTGTCTAC GTGCCCTATG AGATCCGAGT CCAGGCCGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCCGG GGAAGACTAT
    CCCAGGGCTG CACCCACTGA CGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGTCTC
    CAGTGGAACC GCGTTTACCC TGACACAGTC CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC
    AGCTTCCCTG GTGACCGCCC CCGCGGCGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTCGTGGT CAATGGGAGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGACGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCGGAACACC CCAATGGGAT CCTGATCGGA
    TACACTCTCA AATATGTGGC TTTTAATGGA ACCAAATTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGATG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTACCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGGGAAG CAGTCACAGA GGAGTCACCA
    GCACCCCCAA ATGAAGCTAC TCCAACAGCA GCTTACACCA ATAACCAGGC AGACATCGCC
    ACCCAGGGCT GGTTCATTGG GCTCATGTGT GCCATCGCCC TCCTGGTGCT GATCCTGCTC
    ATCGTCTGCT TCATCAAGAG GAGTCGAGGT GGCAAATACC CAGTGCGAGA AAAGAAGGAC
    GTTCCTCTTG GCCCCGAAGA CCCCAAGGAA GAGGATGGCT CCTTTGACTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTCGATG GTACCATCAA GCAGCAAGAG
    AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GGCAGTTCAA TGAGGATGGG
    TCCTTCATCG GCCAGTACAC GGTCAAAAAG GACAAAGAGG AGACAGAGGG CAATGAAAGC
    TCAGAGGCCA CATCGCCTGT CAACGCCATC TATTCTCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45320
    Clone ID Related Accession (Same CDS sequence) XM_850983.4 , XM_850983.5
    Accession Version XM_850983.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurofascin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_850983.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CTATCCCAGC CCCCAACCAT CACCAAGCAG
    TCAGTGAAGG ACCACATCGT GGATCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAG
    GGGAACCCTG CCCCCAGCTT CCACTGGACT CGAAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCAATGAG GAGAAAGTCT GGGACCCTGG TGATCGACTT CCGTAGTGGT
    GGGCGGCCCG AAGAATATGA AGGGGAATAC CAGTGCTTTG CCCGCAACAA ATTTGGCACG
    GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAGTCTC CCCTGTGGCC CAAGGAGAAC
    CTAGACCCAG TCGTGGTTCA AGAAGGTGCT CCTTTGACAC TCCAGTGCAA CCCTCCACCC
    GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACTCAGGAC
    AAGCGTGTCT CTCAGGGCCA CAACGGTGAC CTGTATTTCT CCAACGTGAT GCTTCAGGAT
    ATGCAGACAG ACTACAGCTG CAACGCGCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC
    CACCCTGACA TGTACAGTGC ACGAGGAGTT GCAGAAAGGA CACCCAGCTT CATGTATCCC
    CAGGGCACAG CGAGCAGTCA GATGGTGCTG CGTGGCACGG ATCTTCTGCT GGAGTGCATC
    GCCTCTGGGG TCCCCACGCC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT
    GACAAGGCCA AGTTTGAGAA CTTTAACAAG GCTCTGCGTA TCACCAACGT CTCCGAGGAA
    GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC
    TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATCCTGGCT
    CCCGGTGAGG ACGGGAGACT GGTGTGTCGA GCCAATGGGA ACCCCAAGCC CACTGTCCAG
    TGGATGGTAA ATGGGGAACC ACTGCAATCG GCACCACCCA ACCCAAACCG TGAGGTGGCT
    GGGGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GAGCTGTGTA CCAGTGCAAC
    ACCTCCAACG AGCATGGCTA CCTACTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCA
    CCCCGGATGC TGTCACCCCG GAACCAGCTC ATTAGGGTGA TCCTGTACAA CCGGACACGG
    CTGGACTGCC CATTCTTCGG GTCTCCCATC CCCACACTCC GATGGTTTAA GAACGGGCAG
    GGAAGTAACC TGGATGGTGG TAACTACCAC GTCTACGAGA ATGGCAGTCT GGAAATCAAG
    ATGATCCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA
    GCTGAGAACC AGGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCTGAA
    GACCAGGTGG CCAAAAGGGG CACGACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC
    TCCCTGAAGC TCACGGTCTC CTGGCTGAAG GATGATGAGC CTCTCTACAT AGGAAACAGG
    ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGTGTGG CTGAGCGGGA CCAGGGCAGT
    TACACATGTG TTGCCAGCAC TGAGCTGGAC CAGGACCTGG CCAAGGCCTA CCTCACCGTG
    CTAGGACGGC CAGACCGACC CCGGGACTTG GAGCTCACTG ACCTGGCTGA GAGGAGTGTG
    AGGCTGACCT GGATCCCAGG GGATGATAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAGG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCAGGCAGC
    GTCAACTCTG CCATCCTCCA GTTGTCCCCA TATGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAACGAGG TGGGCAGCAG CCATCCCAGC CTCCCCTCGG AGCGCTACAG GACCAGCGGG
    GCGCCCCCTG AGTCCAATCC CGCCGACGTA AAGGGAGAAG GGACCAGAAA GAACAACATG
    GAGATCACTT GGACGCCCAT GAACGCCACC TCTGCCTTTG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA AACTCGAGAG ACGTGGAACA ACGCCACAGT GTGGGGCTCC
    CGCTACGTGG TGGGCCAGAC CCCTGTCTAC GTGCCCTATG AGATCCGAGT CCAGGCCGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCCGG GGAAGACTAT
    CCCAGGGCTG CACCCACTGA CGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGTCTC
    CAGTGGAACC GCGTTTACCC TGACACAGTC CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC
    AGCTTCCCTG GTGACCGCCC CCGCGGCGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTCGTGGT CAATGGGAGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGACGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCGGAACACC CCAATGGGAT CCTGATCGGA
    TACACTCTCA AATATGTGGC TTTTAATGGA ACCAAATTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGATG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTACCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGGGAAG CAGTCACAGA GGAGTCACCA
    GCACCCCCAA ATGAAGCTAC TCCAACAGCA GCTTACACCA ATAACCAGGC AGACATCGCC
    ACCCAGGGCT GGTTCATTGG GCTCATGTGT GCCATCGCCC TCCTGGTGCT GATCCTGCTC
    ATCGTCTGCT TCATCAAGAG GAGTCGAGGT GGCAAATACC CAGTGCGAGA AAAGAAGGAC
    GTTCCTCTTG GCCCCGAAGA CCCCAAGGAA GAGGATGGCT CCTTTGACTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTCGATG GTACCATCAA GCAGCAAGAG
    AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GGCAGTTCAA TGAGGATGGG
    TCCTTCATCG GCCAGTACAC GGTCAAAAAG GACAAAGAGG AGACAGAGGG CAATGAAAGC
    TCAGAGGCCA CATCGCCTGT CAACGCCATC TATTCTCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_856076.4
    CDS303..3827
    Translation

    Target ORF information:

    RefSeq Version XM_850983.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_850983.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CTATCCCAGC CCCCAACCAT CACCAAGCAG
    TCAGTGAAGG ACCACATCGT GGATCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAG
    GGGAACCCTG CCCCCAGCTT CCACTGGACT CGAAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCAATGAG GAGAAAGTCT GGGACCCTGG TGATCGACTT CCGTAGTGGT
    GGGCGGCCCG AAGAATATGA AGGGGAATAC CAGTGCTTTG CCCGCAACAA ATTTGGCACG
    GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAGTCTC CCCTGTGGCC CAAGGAGAAC
    CTAGACCCAG TCGTGGTTCA AGAAGGTGCT CCTTTGACAC TCCAGTGCAA CCCTCCACCC
    GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACTCAGGAC
    AAGCGTGTCT CTCAGGGCCA CAACGGTGAC CTGTATTTCT CCAACGTGAT GCTTCAGGAT
    ATGCAGACAG ACTACAGCTG CAACGCGCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC
    CACCCTGACA TGTACAGTGC ACGAGGAGTT GCAGAAAGGA CACCCAGCTT CATGTATCCC
    CAGGGCACAG CGAGCAGTCA GATGGTGCTG CGTGGCACGG ATCTTCTGCT GGAGTGCATC
    GCCTCTGGGG TCCCCACGCC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT
    GACAAGGCCA AGTTTGAGAA CTTTAACAAG GCTCTGCGTA TCACCAACGT CTCCGAGGAA
    GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC
    TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATCCTGGCT
    CCCGGTGAGG ACGGGAGACT GGTGTGTCGA GCCAATGGGA ACCCCAAGCC CACTGTCCAG
    TGGATGGTAA ATGGGGAACC ACTGCAATCG GCACCACCCA ACCCAAACCG TGAGGTGGCT
    GGGGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GAGCTGTGTA CCAGTGCAAC
    ACCTCCAACG AGCATGGCTA CCTACTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCA
    CCCCGGATGC TGTCACCCCG GAACCAGCTC ATTAGGGTGA TCCTGTACAA CCGGACACGG
    CTGGACTGCC CATTCTTCGG GTCTCCCATC CCCACACTCC GATGGTTTAA GAACGGGCAG
    GGAAGTAACC TGGATGGTGG TAACTACCAC GTCTACGAGA ATGGCAGTCT GGAAATCAAG
    ATGATCCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA
    GCTGAGAACC AGGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCTGAA
    GACCAGGTGG CCAAAAGGGG CACGACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC
    TCCCTGAAGC TCACGGTCTC CTGGCTGAAG GATGATGAGC CTCTCTACAT AGGAAACAGG
    ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGTGTGG CTGAGCGGGA CCAGGGCAGT
    TACACATGTG TTGCCAGCAC TGAGCTGGAC CAGGACCTGG CCAAGGCCTA CCTCACCGTG
    CTAGGACGGC CAGACCGACC CCGGGACTTG GAGCTCACTG ACCTGGCTGA GAGGAGTGTG
    AGGCTGACCT GGATCCCAGG GGATGATAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAGG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCAGGCAGC
    GTCAACTCTG CCATCCTCCA GTTGTCCCCA TATGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAACGAGG TGGGCAGCAG CCATCCCAGC CTCCCCTCGG AGCGCTACAG GACCAGCGGG
    GCGCCCCCTG AGTCCAATCC CGCCGACGTA AAGGGAGAAG GGACCAGAAA GAACAACATG
    GAGATCACTT GGACGCCCAT GAACGCCACC TCTGCCTTTG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA AACTCGAGAG ACGTGGAACA ACGCCACAGT GTGGGGCTCC
    CGCTACGTGG TGGGCCAGAC CCCTGTCTAC GTGCCCTATG AGATCCGAGT CCAGGCCGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCCGG GGAAGACTAT
    CCCAGGGCTG CACCCACTGA CGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGTCTC
    CAGTGGAACC GCGTTTACCC TGACACAGTC CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC
    AGCTTCCCTG GTGACCGCCC CCGCGGCGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTCGTGGT CAATGGGAGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGACGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCGGAACACC CCAATGGGAT CCTGATCGGA
    TACACTCTCA AATATGTGGC TTTTAATGGA ACCAAATTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGATG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTACCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGGGAAG CAGTCACAGA GGAGTCACCA
    GCACCCCCAA ATGAAGCTAC TCCAACAGCA GCTTACACCA ATAACCAGGC AGACATCGCC
    ACCCAGGGCT GGTTCATTGG GCTCATGTGT GCCATCGCCC TCCTGGTGCT GATCCTGCTC
    ATCGTCTGCT TCATCAAGAG GAGTCGAGGT GGCAAATACC CAGTGCGAGA AAAGAAGGAC
    GTTCCTCTTG GCCCCGAAGA CCCCAAGGAA GAGGATGGCT CCTTTGACTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACG TCCCTCGATG GTACCATCAA GCAGCAAGAG
    AGCGACGACA GCCTGGTGGA CTATGGCGAG GGTGGCGAGG GGCAGTTCAA TGAGGATGGG
    TCCTTCATCG GCCAGTACAC GGTCAAAAAG GACAAAGAGG AGACAGAGGG CAATGAAAGC
    TCAGAGGCCA CATCGCCTGT CAACGCCATC TATTCTCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45321
    Clone ID Related Accession (Same CDS sequence) XM_003434326.4 , XM_003434326.3
    Accession Version XM_003434326.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3510bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurofascin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003434326.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CTATCCCAGC CCCCAACCAT CACCAAGCAG
    TCAGTGAAGG ACCACATCGT GGATCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAG
    GGGAACCCTG CCCCCAGCTT CCACTGGACT CGAAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCAATGAG GAGAAAGTCT GGGACCCTGG TGATCGACTT CCGTAGTGGT
    GGGCGGCCCG AAGAATATGA AGGGGAATAC CAGTGCTTTG CCCGCAACAA ATTTGGCACG
    GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAGTCTC CCCTGTGGCC CAAGGAGAAC
    CTAGACCCAG TCGTGGTTCA AGAAGGTGCT CCTTTGACAC TCCAGTGCAA CCCTCCACCC
    GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACTCAGGAC
    AAGCGTGTCT CTCAGGGCCA CAACGGTGAC CTGTATTTCT CCAACGTGAT GCTTCAGGAT
    ATGCAGACAG ACTACAGCTG CAACGCGCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC
    CACCCTGACA TGTACAGTGC ACGAGGAGTT GCAGAAAGGA CACCCAGCTT CATGTATCCC
    CAGGGCACAG CGAGCAGTCA GATGGTGCTG CGTGGCACGG ATCTTCTGCT GGAGTGCATC
    GCCTCTGGGG TCCCCACGCC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT
    GACAAGGCCA AGTTTGAGAA CTTTAACAAG GCTCTGCGTA TCACCAACGT CTCCGAGGAA
    GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC
    TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATCCTGGCT
    CCCGGTGAGG ACGGGAGACT GGTGTGTCGA GCCAATGGGA ACCCCAAGCC CACTGTCCAG
    TGGATGGTAA ATGGGGAACC ACTGCAATCG GCACCACCCA ACCCAAACCG TGAGGTGGCT
    GGGGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GAGCTGTGTA CCAGTGCAAC
    ACCTCCAACG AGCATGGCTA CCTACTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCA
    CCCCGGATGC TGTCACCCCG GAACCAGCTC ATTAGGGTGA TCCTGTACAA CCGGACACGG
    CTGGACTGCC CATTCTTCGG GTCTCCCATC CCCACACTCC GATGGTTTAA GAACGGGCAG
    GGAAGTAACC TGGATGGTGG TAACTACCAC GTCTACGAGA ATGGCAGTCT GGAAATCAAG
    ATGATCCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA
    GCTGAGAACC AGGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCTGAA
    GACCAGGTGG CCAAAAGGGG CACGACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC
    TCCCTGAAGC TCACGGTCTC CTGGCTGAAG GATGATGAGC CTCTCTACAT AGGAAACAGG
    ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGTGTGG CTGAGCGGGA CCAGGGCAGT
    TACACATGTG TTGCCAGCAC TGAGCTGGAC CAGGACCTGG CCAAGGCCTA CCTCACCGTG
    CTAGGACGGC CAGACCGACC CCGGGACTTG GAGCTCACTG ACCTGGCTGA GAGGAGTGTG
    AGGCTGACCT GGATCCCAGG GGATGATAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAGG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCAGGCAGC
    GTCAACTCTG CCATCCTCCA GTTGTCCCCA TATGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAACGAGG TGGGCAGCAG CCATCCCAGC CTCCCCTCGG AGCGCTACAG GACCAGCGGG
    GCGCCCCCTG AGTCCAATCC CGCCGACGTA AAGGGAGAAG GGACCAGAAA GAACAACATG
    GAGATCACTT GGACGCCCAT GAACGCCACC TCTGCCTTTG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA AACTCGAGAG ACGTGGAACA ACGCCACAGT GTGGGGCTCC
    CGCTACGTGG TGGGCCAGAC CCCTGTCTAC GTGCCCTATG AGATCCGAGT CCAGGCCGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCCGG GGAAGACTAT
    CCCAGGGCTG CACCCACTGA CGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGTCTC
    CAGTGGAACC GCGTTTACCC TGACACAGTC CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC
    AGCTTCCCTG GTGACCGCCC CCGCGGCGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTCGTGGT CAATGGGAGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGACGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCGGAACACC CCAATGGGAT CCTGATCGGA
    TACACTCTCA AATATGTGGC TTTTAATGGA ACCAAATTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGATG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTACCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGGGAAG CAGTCACAGA GGAGTCACCA
    GCACCCCCAA ATGAAGCTTA CACCAATAAC CAGGCAGACA TCGCCACCCA GGGCTGGTTC
    ATTGGGCTCA TGTGTGCCAT CGCCCTCCTG GTGCTGATCC TGCTCATCGT CTGCTTCATC
    AAGAGGAGTC GAGGTGGCAA ATACCCAGTG CGAGAAAAGA AGGACGTTCC TCTTGGCCCC
    GAAGACCCCA AGGAAGAGGA TGGCTCCTTT GACTACAGTG ACGAGGACAA CAAGCCCCTG
    CAGGGCAGCC AGACGTCCCT CGATGGTACC ATCAAGCAGC AAGAGAGCGA CGACAGCCTG
    GTGGACTATG GCGAGGGTGG CGAGGGGCAG TTCAATGAGG ATGGGTCCTT CATCGGCCAG
    TACACGGTCA AAAAGGACAA AGAGGAGACA GAGGGCAATG AAAGCTCAGA GGCCACATCG
    CCTGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003434374.3
    CDS303..3812
    Translation

    Target ORF information:

    RefSeq Version XM_003434326.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003434326.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CTATCCCAGC CCCCAACCAT CACCAAGCAG
    TCAGTGAAGG ACCACATCGT GGATCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAG
    GGGAACCCTG CCCCCAGCTT CCACTGGACT CGAAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCAATGAG GAGAAAGTCT GGGACCCTGG TGATCGACTT CCGTAGTGGT
    GGGCGGCCCG AAGAATATGA AGGGGAATAC CAGTGCTTTG CCCGCAACAA ATTTGGCACG
    GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAGTCTC CCCTGTGGCC CAAGGAGAAC
    CTAGACCCAG TCGTGGTTCA AGAAGGTGCT CCTTTGACAC TCCAGTGCAA CCCTCCACCC
    GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACTCAGGAC
    AAGCGTGTCT CTCAGGGCCA CAACGGTGAC CTGTATTTCT CCAACGTGAT GCTTCAGGAT
    ATGCAGACAG ACTACAGCTG CAACGCGCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC
    CACCCTGACA TGTACAGTGC ACGAGGAGTT GCAGAAAGGA CACCCAGCTT CATGTATCCC
    CAGGGCACAG CGAGCAGTCA GATGGTGCTG CGTGGCACGG ATCTTCTGCT GGAGTGCATC
    GCCTCTGGGG TCCCCACGCC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT
    GACAAGGCCA AGTTTGAGAA CTTTAACAAG GCTCTGCGTA TCACCAACGT CTCCGAGGAA
    GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC
    TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATCCTGGCT
    CCCGGTGAGG ACGGGAGACT GGTGTGTCGA GCCAATGGGA ACCCCAAGCC CACTGTCCAG
    TGGATGGTAA ATGGGGAACC ACTGCAATCG GCACCACCCA ACCCAAACCG TGAGGTGGCT
    GGGGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GAGCTGTGTA CCAGTGCAAC
    ACCTCCAACG AGCATGGCTA CCTACTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCA
    CCCCGGATGC TGTCACCCCG GAACCAGCTC ATTAGGGTGA TCCTGTACAA CCGGACACGG
    CTGGACTGCC CATTCTTCGG GTCTCCCATC CCCACACTCC GATGGTTTAA GAACGGGCAG
    GGAAGTAACC TGGATGGTGG TAACTACCAC GTCTACGAGA ATGGCAGTCT GGAAATCAAG
    ATGATCCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA
    GCTGAGAACC AGGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCTGAA
    GACCAGGTGG CCAAAAGGGG CACGACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC
    TCCCTGAAGC TCACGGTCTC CTGGCTGAAG GATGATGAGC CTCTCTACAT AGGAAACAGG
    ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGTGTGG CTGAGCGGGA CCAGGGCAGT
    TACACATGTG TTGCCAGCAC TGAGCTGGAC CAGGACCTGG CCAAGGCCTA CCTCACCGTG
    CTAGGACGGC CAGACCGACC CCGGGACTTG GAGCTCACTG ACCTGGCTGA GAGGAGTGTG
    AGGCTGACCT GGATCCCAGG GGATGATAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAGG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCAGGCAGC
    GTCAACTCTG CCATCCTCCA GTTGTCCCCA TATGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAACGAGG TGGGCAGCAG CCATCCCAGC CTCCCCTCGG AGCGCTACAG GACCAGCGGG
    GCGCCCCCTG AGTCCAATCC CGCCGACGTA AAGGGAGAAG GGACCAGAAA GAACAACATG
    GAGATCACTT GGACGCCCAT GAACGCCACC TCTGCCTTTG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA AACTCGAGAG ACGTGGAACA ACGCCACAGT GTGGGGCTCC
    CGCTACGTGG TGGGCCAGAC CCCTGTCTAC GTGCCCTATG AGATCCGAGT CCAGGCCGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCCGG GGAAGACTAT
    CCCAGGGCTG CACCCACTGA CGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGTCTC
    CAGTGGAACC GCGTTTACCC TGACACAGTC CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC
    AGCTTCCCTG GTGACCGCCC CCGCGGCGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTCGTGGT CAATGGGAGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGACGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCGGAACACC CCAATGGGAT CCTGATCGGA
    TACACTCTCA AATATGTGGC TTTTAATGGA ACCAAATTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGATG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTACCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGGGAAG CAGTCACAGA GGAGTCACCA
    GCACCCCCAA ATGAAGCTTA CACCAATAAC CAGGCAGACA TCGCCACCCA GGGCTGGTTC
    ATTGGGCTCA TGTGTGCCAT CGCCCTCCTG GTGCTGATCC TGCTCATCGT CTGCTTCATC
    AAGAGGAGTC GAGGTGGCAA ATACCCAGTG CGAGAAAAGA AGGACGTTCC TCTTGGCCCC
    GAAGACCCCA AGGAAGAGGA TGGCTCCTTT GACTACAGTG ACGAGGACAA CAAGCCCCTG
    CAGGGCAGCC AGACGTCCCT CGATGGTACC ATCAAGCAGC AAGAGAGCGA CGACAGCCTG
    GTGGACTATG GCGAGGGTGG CGAGGGGCAG TTCAATGAGG ATGGGTCCTT CATCGGCCAG
    TACACGGTCA AAAAGGACAA AGAGGAGACA GAGGGCAATG AAAGCTCAGA GGCCACATCG
    CCTGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45321
    Clone ID Related Accession (Same CDS sequence) XM_003434326.4 , XM_003434326.3
    Accession Version XM_003434326.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3510bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neurofascin isoform X14
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003434326.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CTATCCCAGC CCCCAACCAT CACCAAGCAG
    TCAGTGAAGG ACCACATCGT GGATCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAG
    GGGAACCCTG CCCCCAGCTT CCACTGGACT CGAAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCAATGAG GAGAAAGTCT GGGACCCTGG TGATCGACTT CCGTAGTGGT
    GGGCGGCCCG AAGAATATGA AGGGGAATAC CAGTGCTTTG CCCGCAACAA ATTTGGCACG
    GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAGTCTC CCCTGTGGCC CAAGGAGAAC
    CTAGACCCAG TCGTGGTTCA AGAAGGTGCT CCTTTGACAC TCCAGTGCAA CCCTCCACCC
    GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACTCAGGAC
    AAGCGTGTCT CTCAGGGCCA CAACGGTGAC CTGTATTTCT CCAACGTGAT GCTTCAGGAT
    ATGCAGACAG ACTACAGCTG CAACGCGCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC
    CACCCTGACA TGTACAGTGC ACGAGGAGTT GCAGAAAGGA CACCCAGCTT CATGTATCCC
    CAGGGCACAG CGAGCAGTCA GATGGTGCTG CGTGGCACGG ATCTTCTGCT GGAGTGCATC
    GCCTCTGGGG TCCCCACGCC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT
    GACAAGGCCA AGTTTGAGAA CTTTAACAAG GCTCTGCGTA TCACCAACGT CTCCGAGGAA
    GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC
    TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATCCTGGCT
    CCCGGTGAGG ACGGGAGACT GGTGTGTCGA GCCAATGGGA ACCCCAAGCC CACTGTCCAG
    TGGATGGTAA ATGGGGAACC ACTGCAATCG GCACCACCCA ACCCAAACCG TGAGGTGGCT
    GGGGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GAGCTGTGTA CCAGTGCAAC
    ACCTCCAACG AGCATGGCTA CCTACTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCA
    CCCCGGATGC TGTCACCCCG GAACCAGCTC ATTAGGGTGA TCCTGTACAA CCGGACACGG
    CTGGACTGCC CATTCTTCGG GTCTCCCATC CCCACACTCC GATGGTTTAA GAACGGGCAG
    GGAAGTAACC TGGATGGTGG TAACTACCAC GTCTACGAGA ATGGCAGTCT GGAAATCAAG
    ATGATCCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA
    GCTGAGAACC AGGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCTGAA
    GACCAGGTGG CCAAAAGGGG CACGACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC
    TCCCTGAAGC TCACGGTCTC CTGGCTGAAG GATGATGAGC CTCTCTACAT AGGAAACAGG
    ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGTGTGG CTGAGCGGGA CCAGGGCAGT
    TACACATGTG TTGCCAGCAC TGAGCTGGAC CAGGACCTGG CCAAGGCCTA CCTCACCGTG
    CTAGGACGGC CAGACCGACC CCGGGACTTG GAGCTCACTG ACCTGGCTGA GAGGAGTGTG
    AGGCTGACCT GGATCCCAGG GGATGATAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAGG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCAGGCAGC
    GTCAACTCTG CCATCCTCCA GTTGTCCCCA TATGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAACGAGG TGGGCAGCAG CCATCCCAGC CTCCCCTCGG AGCGCTACAG GACCAGCGGG
    GCGCCCCCTG AGTCCAATCC CGCCGACGTA AAGGGAGAAG GGACCAGAAA GAACAACATG
    GAGATCACTT GGACGCCCAT GAACGCCACC TCTGCCTTTG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA AACTCGAGAG ACGTGGAACA ACGCCACAGT GTGGGGCTCC
    CGCTACGTGG TGGGCCAGAC CCCTGTCTAC GTGCCCTATG AGATCCGAGT CCAGGCCGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCCGG GGAAGACTAT
    CCCAGGGCTG CACCCACTGA CGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGTCTC
    CAGTGGAACC GCGTTTACCC TGACACAGTC CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC
    AGCTTCCCTG GTGACCGCCC CCGCGGCGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTCGTGGT CAATGGGAGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGACGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCGGAACACC CCAATGGGAT CCTGATCGGA
    TACACTCTCA AATATGTGGC TTTTAATGGA ACCAAATTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGATG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTACCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGGGAAG CAGTCACAGA GGAGTCACCA
    GCACCCCCAA ATGAAGCTTA CACCAATAAC CAGGCAGACA TCGCCACCCA GGGCTGGTTC
    ATTGGGCTCA TGTGTGCCAT CGCCCTCCTG GTGCTGATCC TGCTCATCGT CTGCTTCATC
    AAGAGGAGTC GAGGTGGCAA ATACCCAGTG CGAGAAAAGA AGGACGTTCC TCTTGGCCCC
    GAAGACCCCA AGGAAGAGGA TGGCTCCTTT GACTACAGTG ACGAGGACAA CAAGCCCCTG
    CAGGGCAGCC AGACGTCCCT CGATGGTACC ATCAAGCAGC AAGAGAGCGA CGACAGCCTG
    GTGGACTATG GCGAGGGTGG CGAGGGGCAG TTCAATGAGG ATGGGTCCTT CATCGGCCAG
    TACACGGTCA AAAAGGACAA AGAGGAGACA GAGGGCAATG AAAGCTCAGA GGCCACATCG
    CCTGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003434374.3
    CDS287..3796
    Translation

    Target ORF information:

    RefSeq Version XM_003434326.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003434326.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CTATCCCAGC CCCCAACCAT CACCAAGCAG
    TCAGTGAAGG ACCACATCGT GGATCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAG
    GGGAACCCTG CCCCCAGCTT CCACTGGACT CGAAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCAATGAG GAGAAAGTCT GGGACCCTGG TGATCGACTT CCGTAGTGGT
    GGGCGGCCCG AAGAATATGA AGGGGAATAC CAGTGCTTTG CCCGCAACAA ATTTGGCACG
    GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAGTCTC CCCTGTGGCC CAAGGAGAAC
    CTAGACCCAG TCGTGGTTCA AGAAGGTGCT CCTTTGACAC TCCAGTGCAA CCCTCCACCC
    GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACTCAGGAC
    AAGCGTGTCT CTCAGGGCCA CAACGGTGAC CTGTATTTCT CCAACGTGAT GCTTCAGGAT
    ATGCAGACAG ACTACAGCTG CAACGCGCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC
    CACCCTGACA TGTACAGTGC ACGAGGAGTT GCAGAAAGGA CACCCAGCTT CATGTATCCC
    CAGGGCACAG CGAGCAGTCA GATGGTGCTG CGTGGCACGG ATCTTCTGCT GGAGTGCATC
    GCCTCTGGGG TCCCCACGCC AGACATCGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT
    GACAAGGCCA AGTTTGAGAA CTTTAACAAG GCTCTGCGTA TCACCAACGT CTCCGAGGAA
    GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC
    TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGACGAAC CCAAGAACCT TATCCTGGCT
    CCCGGTGAGG ACGGGAGACT GGTGTGTCGA GCCAATGGGA ACCCCAAGCC CACTGTCCAG
    TGGATGGTAA ATGGGGAACC ACTGCAATCG GCACCACCCA ACCCAAACCG TGAGGTGGCT
    GGGGACACCA TCATCTTCCG GGACACCCAG ATCAGCAGCA GAGCTGTGTA CCAGTGCAAC
    ACCTCCAACG AGCATGGCTA CCTACTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCA
    CCCCGGATGC TGTCACCCCG GAACCAGCTC ATTAGGGTGA TCCTGTACAA CCGGACACGG
    CTGGACTGCC CATTCTTCGG GTCTCCCATC CCCACACTCC GATGGTTTAA GAACGGGCAG
    GGAAGTAACC TGGATGGTGG TAACTACCAC GTCTACGAGA ATGGCAGTCT GGAAATCAAG
    ATGATCCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTCG CCACCAACAT CCTGGGCAAA
    GCTGAGAACC AGGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACCG GATGCCTGAA
    GACCAGGTGG CCAAAAGGGG CACGACGGTG CAGCTGGAGT GTCGGGTGAA GCACGACCCC
    TCCCTGAAGC TCACGGTCTC CTGGCTGAAG GATGATGAGC CTCTCTACAT AGGAAACAGG
    ATGAAGAAGG AAGACGACTC CCTGACCATC TTTGGTGTGG CTGAGCGGGA CCAGGGCAGT
    TACACATGTG TTGCCAGCAC TGAGCTGGAC CAGGACCTGG CCAAGGCCTA CCTCACCGTG
    CTAGGACGGC CAGACCGACC CCGGGACTTG GAGCTCACTG ACCTGGCTGA GAGGAGTGTG
    AGGCTGACCT GGATCCCAGG GGATGATAAC AACAGCCCCA TCACAGACTA CGTCGTCCAG
    TTTGAAGAGG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCAGGCAGC
    GTCAACTCTG CCATCCTCCA GTTGTCCCCA TATGTCAACT ACCAGTTCCG GGTCATCGCC
    ATCAACGAGG TGGGCAGCAG CCATCCCAGC CTCCCCTCGG AGCGCTACAG GACCAGCGGG
    GCGCCCCCTG AGTCCAATCC CGCCGACGTA AAGGGAGAAG GGACCAGAAA GAACAACATG
    GAGATCACTT GGACGCCCAT GAACGCCACC TCTGCCTTTG GCCCCAACCT GCGCTACATC
    GTCAAGTGGC GGCGGAGAGA AACTCGAGAG ACGTGGAACA ACGCCACAGT GTGGGGCTCC
    CGCTACGTGG TGGGCCAGAC CCCTGTCTAC GTGCCCTATG AGATCCGAGT CCAGGCCGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACTGTCATCG GTTACTCCGG GGAAGACTAT
    CCCAGGGCTG CACCCACTGA CGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGTCTC
    CAGTGGAACC GCGTTTACCC TGACACAGTC CAGGGCCAGC TCAGAGAGTA CCGAGCCTAC
    TACTGGAGGG AGAGCAGCTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAAGCC
    AGCTTCCCTG GTGACCGCCC CCGCGGCGTG GTGTCCCGCC TCTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTCGTGGT CAATGGGAGA GGCGATGGGC CTCGCAGTGA AACCAAGGAG
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGACGTT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACAA TCAACCTGGA ATGGGATCAT CCGGAACACC CCAATGGGAT CCTGATCGGA
    TACACTCTCA AATATGTGGC TTTTAATGGA ACCAAATTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGATG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTACCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGGGAAG CAGTCACAGA GGAGTCACCA
    GCACCCCCAA ATGAAGCTTA CACCAATAAC CAGGCAGACA TCGCCACCCA GGGCTGGTTC
    ATTGGGCTCA TGTGTGCCAT CGCCCTCCTG GTGCTGATCC TGCTCATCGT CTGCTTCATC
    AAGAGGAGTC GAGGTGGCAA ATACCCAGTG CGAGAAAAGA AGGACGTTCC TCTTGGCCCC
    GAAGACCCCA AGGAAGAGGA TGGCTCCTTT GACTACAGTG ACGAGGACAA CAAGCCCCTG
    CAGGGCAGCC AGACGTCCCT CGATGGTACC ATCAAGCAGC AAGAGAGCGA CGACAGCCTG
    GTGGACTATG GCGAGGGTGG CGAGGGGCAG TTCAATGAGG ATGGGTCCTT CATCGGCCAG
    TACACGGTCA AAAAGGACAA AGAGGAGACA GAGGGCAATG AAAGCTCAGA GGCCACATCG
    CCTGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45322
    Clone ID Related Accession (Same CDS sequence) XM_005640782.2 , XM_005640782.3
    Accession Version XM_005640782.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neurofascin isoform X15
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640782.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CTATCCCAGC CCCCAACCAT CACCAAGCAG
    TCAGTGAAGG ACCACATCGT GGATCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAG
    GGGAACCCTG CCCCCAGCTT CCACTGGACT CGAAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCAATGAG GAGAAAGTCT GGGACCCTGG TGATCGACTT CCGTAGTGGT
    GGGCGGCCCG AAGAATATGA AGGGGAATAC CAGTGCTTTG CCCGCAACAA ATTTGGCACG
    GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAGTCTC CCCTGTGGCC CAAGGAGAAC
    CTAGACCCAG TCGTGGTTCA AGAAGGTGCT CCTTTGACAC TCCAGTGCAA CCCTCCACCC
    GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACTCAGGAC
    AAGCGTGTCT CTCAGGGCCA CAACGGTGAC CTGTATTTCT CCAACGTGAT GCTTCAGGAT
    ATGCAGACAG ACTACAGCTG CAACGCGCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCACA CGAGGAGTTG CAGAAAGGAC ACCCAGCTTC
    ATGTATCCCC AGGGCACAGC GAGCAGTCAG ATGGTGCTGC GTGGCACGGA TCTTCTGCTG
    GAGTGCATCG CCTCTGGGGT CCCCACGCCA GACATCGCAT GGTACAAGAA AGGTGGGGAC
    CTCCCATCTG ACAAGGCCAA GTTTGAGAAC TTTAACAAGG CTCTGCGTAT CACCAACGTC
    TCCGAGGAAG ACTCTGGGGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG
    CACACGATCT CGGTGAGAGT AAAGGCTGCT CCCTACTGGC TGGACGAACC CAAGAACCTT
    ATCCTGGCTC CCGGTGAGGA CGGGAGACTG GTGTGTCGAG CCAATGGGAA CCCCAAGCCC
    ACTGTCCAGT GGATGGTAAA TGGGGAACCA CTGCAATCGG CACCACCCAA CCCAAACCGT
    GAGGTGGCTG GGGACACCAT CATCTTCCGG GACACCCAGA TCAGCAGCAG AGCTGTGTAC
    CAGTGCAACA CCTCCAACGA GCATGGCTAC CTACTGGCCA ACGCCTTCGT CAGCGTGCTG
    GACGTGCCAC CCCGGATGCT GTCACCCCGG AACCAGCTCA TTAGGGTGAT CCTGTACAAC
    CGGACACGGC TGGACTGCCC ATTCTTCGGG TCTCCCATCC CCACACTCCG ATGGTTTAAG
    AACGGGCAGG GAAGTAACCT GGATGGTGGT AACTACCACG TCTACGAGAA TGGCAGTCTG
    GAAATCAAGA TGATCCGCAA AGAGGACCAA GGCATCTACA CCTGTGTCGC CACCAACATC
    CTGGGCAAAG CTGAGAACCA GGTCCGCCTG GAGGTCAAAG ACCCCACCAG GATCTACCGG
    ATGCCTGAAG ACCAGGTGGC CAAAAGGGGC ACGACGGTGC AGCTGGAGTG TCGGGTGAAG
    CACGACCCCT CCCTGAAGCT CACGGTCTCC TGGCTGAAGG ATGATGAGCC TCTCTACATA
    GGAAACAGGA TGAAGAAGGA AGACGACTCC CTGACCATCT TTGGTGTGGC TGAGCGGGAC
    CAGGGCAGTT ACACATGTGT TGCCAGCACT GAGCTGGACC AGGACCTGGC CAAGGCCTAC
    CTCACCGTGC TAGGACGGCC AGACCGACCC CGGGACTTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGATAACA ACAGCCCCAT CACAGACTAC
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGCG TCAACTCTGC CATCCTCCAG TTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATCGCCA TCAACGAGGT GGGCAGCAGC CATCCCAGCC TCCCCTCGGA GCGCTACAGG
    ACCAGCGGGG CGCCCCCTGA GTCCAATCCC GCCGACGTAA AGGGAGAAGG GACCAGAAAG
    AACAACATGG AGATCACTTG GACGCCCATG AACGCCACCT CTGCCTTTGG CCCCAACCTG
    CGCTACATCG TCAAGTGGCG GCGGAGAGAA ACTCGAGAGA CGTGGAACAA CGCCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGCCAGACC CCTGTCTACG TGCCCTATGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CTGTCATCGG TTACTCCGGG
    GAAGACTATC CCAGGGCTGC ACCCACTGAC GTTAAAATCC GAGTCCTGAA CAGCACAGCC
    ATCAGTCTCC AGTGGAACCG CGTTTACCCT GACACAGTCC AGGGCCAGCT CAGAGAGTAC
    CGAGCCTACT ACTGGAGGGA GAGCAGCTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA
    CAGCAAGCCA GCTTCCCTGG TGACCGCCCC CGCGGCGTGG TGTCCCGCCT CTTCCCCTAC
    AGTAACTACA AGCTGGAGAT GGTCGTGGTC AATGGGAGAG GCGATGGGCC TCGCAGTGAA
    ACCAAGGAGT TCACCACCCC GGAAGGAGTA CCCAGTGCCC CCAGACGTTT CCGAGTCCGG
    CAGCCCAACC TGGAGACAAT CAACCTGGAA TGGGATCATC CGGAACACCC CAATGGGATC
    CTGATCGGAT ACACTCTCAA ATATGTGGCT TTTAATGGAA CCAAATTAGG AAAGCAGATA
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGATGC AAAGAGCAGA CCCCGTGTCG
    CGCTACCGCT TTACCCTCAG TGCCAGGACG CAGGTGGGCT CTGGGGAAGC AGTCACAGAG
    GAGTCACCAG CACCCCCAAA TGAAGCTACT CCAACAGCAG CTTACACCAA TAACCAGGCA
    GACATCGCCA CCCAGGGCTG GTTCATTGGG CTCATGTGTG CCATCGCCCT CCTGGTGCTG
    ATCCTGCTCA TCGTCTGCTT CATCAAGAGG AGTCGAGGTG GCAAATACCC AGTGCGAGAA
    AAGAAGGACG TTCCTCTTGG CCCCGAAGAC CCCAAGGAAG AGGATGGCTC CTTTGACTAC
    AGTGACGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACGT CCCTCGATGG TACCATCAAG
    CAGCAAGAGA GCGACGACAG CCTGGTGGAC TATGGCGAGG GTGGCGAGGG GCAGTTCAAT
    GAGGATGGGT CCTTCATCGG CCAGTACACG GTCAAAAAGG ACAAAGAGGA GACAGAGGGC
    AATGAAAGCT CAGAGGCCAC ATCGCCTGTC AACGCCATCT ATTCTCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640839.2
    CDS287..3760
    Translation

    Target ORF information:

    RefSeq Version XM_005640782.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640782.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CTATCCCAGC CCCCAACCAT CACCAAGCAG
    TCAGTGAAGG ACCACATCGT GGATCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAG
    GGGAACCCTG CCCCCAGCTT CCACTGGACT CGAAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCAATGAG GAGAAAGTCT GGGACCCTGG TGATCGACTT CCGTAGTGGT
    GGGCGGCCCG AAGAATATGA AGGGGAATAC CAGTGCTTTG CCCGCAACAA ATTTGGCACG
    GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAGTCTC CCCTGTGGCC CAAGGAGAAC
    CTAGACCCAG TCGTGGTTCA AGAAGGTGCT CCTTTGACAC TCCAGTGCAA CCCTCCACCC
    GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACTCAGGAC
    AAGCGTGTCT CTCAGGGCCA CAACGGTGAC CTGTATTTCT CCAACGTGAT GCTTCAGGAT
    ATGCAGACAG ACTACAGCTG CAACGCGCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCACA CGAGGAGTTG CAGAAAGGAC ACCCAGCTTC
    ATGTATCCCC AGGGCACAGC GAGCAGTCAG ATGGTGCTGC GTGGCACGGA TCTTCTGCTG
    GAGTGCATCG CCTCTGGGGT CCCCACGCCA GACATCGCAT GGTACAAGAA AGGTGGGGAC
    CTCCCATCTG ACAAGGCCAA GTTTGAGAAC TTTAACAAGG CTCTGCGTAT CACCAACGTC
    TCCGAGGAAG ACTCTGGGGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG
    CACACGATCT CGGTGAGAGT AAAGGCTGCT CCCTACTGGC TGGACGAACC CAAGAACCTT
    ATCCTGGCTC CCGGTGAGGA CGGGAGACTG GTGTGTCGAG CCAATGGGAA CCCCAAGCCC
    ACTGTCCAGT GGATGGTAAA TGGGGAACCA CTGCAATCGG CACCACCCAA CCCAAACCGT
    GAGGTGGCTG GGGACACCAT CATCTTCCGG GACACCCAGA TCAGCAGCAG AGCTGTGTAC
    CAGTGCAACA CCTCCAACGA GCATGGCTAC CTACTGGCCA ACGCCTTCGT CAGCGTGCTG
    GACGTGCCAC CCCGGATGCT GTCACCCCGG AACCAGCTCA TTAGGGTGAT CCTGTACAAC
    CGGACACGGC TGGACTGCCC ATTCTTCGGG TCTCCCATCC CCACACTCCG ATGGTTTAAG
    AACGGGCAGG GAAGTAACCT GGATGGTGGT AACTACCACG TCTACGAGAA TGGCAGTCTG
    GAAATCAAGA TGATCCGCAA AGAGGACCAA GGCATCTACA CCTGTGTCGC CACCAACATC
    CTGGGCAAAG CTGAGAACCA GGTCCGCCTG GAGGTCAAAG ACCCCACCAG GATCTACCGG
    ATGCCTGAAG ACCAGGTGGC CAAAAGGGGC ACGACGGTGC AGCTGGAGTG TCGGGTGAAG
    CACGACCCCT CCCTGAAGCT CACGGTCTCC TGGCTGAAGG ATGATGAGCC TCTCTACATA
    GGAAACAGGA TGAAGAAGGA AGACGACTCC CTGACCATCT TTGGTGTGGC TGAGCGGGAC
    CAGGGCAGTT ACACATGTGT TGCCAGCACT GAGCTGGACC AGGACCTGGC CAAGGCCTAC
    CTCACCGTGC TAGGACGGCC AGACCGACCC CGGGACTTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGATAACA ACAGCCCCAT CACAGACTAC
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGCG TCAACTCTGC CATCCTCCAG TTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATCGCCA TCAACGAGGT GGGCAGCAGC CATCCCAGCC TCCCCTCGGA GCGCTACAGG
    ACCAGCGGGG CGCCCCCTGA GTCCAATCCC GCCGACGTAA AGGGAGAAGG GACCAGAAAG
    AACAACATGG AGATCACTTG GACGCCCATG AACGCCACCT CTGCCTTTGG CCCCAACCTG
    CGCTACATCG TCAAGTGGCG GCGGAGAGAA ACTCGAGAGA CGTGGAACAA CGCCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGCCAGACC CCTGTCTACG TGCCCTATGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CTGTCATCGG TTACTCCGGG
    GAAGACTATC CCAGGGCTGC ACCCACTGAC GTTAAAATCC GAGTCCTGAA CAGCACAGCC
    ATCAGTCTCC AGTGGAACCG CGTTTACCCT GACACAGTCC AGGGCCAGCT CAGAGAGTAC
    CGAGCCTACT ACTGGAGGGA GAGCAGCTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA
    CAGCAAGCCA GCTTCCCTGG TGACCGCCCC CGCGGCGTGG TGTCCCGCCT CTTCCCCTAC
    AGTAACTACA AGCTGGAGAT GGTCGTGGTC AATGGGAGAG GCGATGGGCC TCGCAGTGAA
    ACCAAGGAGT TCACCACCCC GGAAGGAGTA CCCAGTGCCC CCAGACGTTT CCGAGTCCGG
    CAGCCCAACC TGGAGACAAT CAACCTGGAA TGGGATCATC CGGAACACCC CAATGGGATC
    CTGATCGGAT ACACTCTCAA ATATGTGGCT TTTAATGGAA CCAAATTAGG AAAGCAGATA
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGATGC AAAGAGCAGA CCCCGTGTCG
    CGCTACCGCT TTACCCTCAG TGCCAGGACG CAGGTGGGCT CTGGGGAAGC AGTCACAGAG
    GAGTCACCAG CACCCCCAAA TGAAGCTACT CCAACAGCAG CTTACACCAA TAACCAGGCA
    GACATCGCCA CCCAGGGCTG GTTCATTGGG CTCATGTGTG CCATCGCCCT CCTGGTGCTG
    ATCCTGCTCA TCGTCTGCTT CATCAAGAGG AGTCGAGGTG GCAAATACCC AGTGCGAGAA
    AAGAAGGACG TTCCTCTTGG CCCCGAAGAC CCCAAGGAAG AGGATGGCTC CTTTGACTAC
    AGTGACGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACGT CCCTCGATGG TACCATCAAG
    CAGCAAGAGA GCGACGACAG CCTGGTGGAC TATGGCGAGG GTGGCGAGGG GCAGTTCAAT
    GAGGATGGGT CCTTCATCGG CCAGTACACG GTCAAAAAGG ACAAAGAGGA GACAGAGGGC
    AATGAAAGCT CAGAGGCCAC ATCGCCTGTC AACGCCATCT ATTCTCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45322
    Clone ID Related Accession (Same CDS sequence) XM_005640782.2 , XM_005640782.3
    Accession Version XM_005640782.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurofascin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005640782.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CTATCCCAGC CCCCAACCAT CACCAAGCAG
    TCAGTGAAGG ACCACATCGT GGATCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAG
    GGGAACCCTG CCCCCAGCTT CCACTGGACT CGAAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCAATGAG GAGAAAGTCT GGGACCCTGG TGATCGACTT CCGTAGTGGT
    GGGCGGCCCG AAGAATATGA AGGGGAATAC CAGTGCTTTG CCCGCAACAA ATTTGGCACG
    GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAGTCTC CCCTGTGGCC CAAGGAGAAC
    CTAGACCCAG TCGTGGTTCA AGAAGGTGCT CCTTTGACAC TCCAGTGCAA CCCTCCACCC
    GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACTCAGGAC
    AAGCGTGTCT CTCAGGGCCA CAACGGTGAC CTGTATTTCT CCAACGTGAT GCTTCAGGAT
    ATGCAGACAG ACTACAGCTG CAACGCGCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCACA CGAGGAGTTG CAGAAAGGAC ACCCAGCTTC
    ATGTATCCCC AGGGCACAGC GAGCAGTCAG ATGGTGCTGC GTGGCACGGA TCTTCTGCTG
    GAGTGCATCG CCTCTGGGGT CCCCACGCCA GACATCGCAT GGTACAAGAA AGGTGGGGAC
    CTCCCATCTG ACAAGGCCAA GTTTGAGAAC TTTAACAAGG CTCTGCGTAT CACCAACGTC
    TCCGAGGAAG ACTCTGGGGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG
    CACACGATCT CGGTGAGAGT AAAGGCTGCT CCCTACTGGC TGGACGAACC CAAGAACCTT
    ATCCTGGCTC CCGGTGAGGA CGGGAGACTG GTGTGTCGAG CCAATGGGAA CCCCAAGCCC
    ACTGTCCAGT GGATGGTAAA TGGGGAACCA CTGCAATCGG CACCACCCAA CCCAAACCGT
    GAGGTGGCTG GGGACACCAT CATCTTCCGG GACACCCAGA TCAGCAGCAG AGCTGTGTAC
    CAGTGCAACA CCTCCAACGA GCATGGCTAC CTACTGGCCA ACGCCTTCGT CAGCGTGCTG
    GACGTGCCAC CCCGGATGCT GTCACCCCGG AACCAGCTCA TTAGGGTGAT CCTGTACAAC
    CGGACACGGC TGGACTGCCC ATTCTTCGGG TCTCCCATCC CCACACTCCG ATGGTTTAAG
    AACGGGCAGG GAAGTAACCT GGATGGTGGT AACTACCACG TCTACGAGAA TGGCAGTCTG
    GAAATCAAGA TGATCCGCAA AGAGGACCAA GGCATCTACA CCTGTGTCGC CACCAACATC
    CTGGGCAAAG CTGAGAACCA GGTCCGCCTG GAGGTCAAAG ACCCCACCAG GATCTACCGG
    ATGCCTGAAG ACCAGGTGGC CAAAAGGGGC ACGACGGTGC AGCTGGAGTG TCGGGTGAAG
    CACGACCCCT CCCTGAAGCT CACGGTCTCC TGGCTGAAGG ATGATGAGCC TCTCTACATA
    GGAAACAGGA TGAAGAAGGA AGACGACTCC CTGACCATCT TTGGTGTGGC TGAGCGGGAC
    CAGGGCAGTT ACACATGTGT TGCCAGCACT GAGCTGGACC AGGACCTGGC CAAGGCCTAC
    CTCACCGTGC TAGGACGGCC AGACCGACCC CGGGACTTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGATAACA ACAGCCCCAT CACAGACTAC
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGCG TCAACTCTGC CATCCTCCAG TTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATCGCCA TCAACGAGGT GGGCAGCAGC CATCCCAGCC TCCCCTCGGA GCGCTACAGG
    ACCAGCGGGG CGCCCCCTGA GTCCAATCCC GCCGACGTAA AGGGAGAAGG GACCAGAAAG
    AACAACATGG AGATCACTTG GACGCCCATG AACGCCACCT CTGCCTTTGG CCCCAACCTG
    CGCTACATCG TCAAGTGGCG GCGGAGAGAA ACTCGAGAGA CGTGGAACAA CGCCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGCCAGACC CCTGTCTACG TGCCCTATGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CTGTCATCGG TTACTCCGGG
    GAAGACTATC CCAGGGCTGC ACCCACTGAC GTTAAAATCC GAGTCCTGAA CAGCACAGCC
    ATCAGTCTCC AGTGGAACCG CGTTTACCCT GACACAGTCC AGGGCCAGCT CAGAGAGTAC
    CGAGCCTACT ACTGGAGGGA GAGCAGCTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA
    CAGCAAGCCA GCTTCCCTGG TGACCGCCCC CGCGGCGTGG TGTCCCGCCT CTTCCCCTAC
    AGTAACTACA AGCTGGAGAT GGTCGTGGTC AATGGGAGAG GCGATGGGCC TCGCAGTGAA
    ACCAAGGAGT TCACCACCCC GGAAGGAGTA CCCAGTGCCC CCAGACGTTT CCGAGTCCGG
    CAGCCCAACC TGGAGACAAT CAACCTGGAA TGGGATCATC CGGAACACCC CAATGGGATC
    CTGATCGGAT ACACTCTCAA ATATGTGGCT TTTAATGGAA CCAAATTAGG AAAGCAGATA
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGATGC AAAGAGCAGA CCCCGTGTCG
    CGCTACCGCT TTACCCTCAG TGCCAGGACG CAGGTGGGCT CTGGGGAAGC AGTCACAGAG
    GAGTCACCAG CACCCCCAAA TGAAGCTACT CCAACAGCAG CTTACACCAA TAACCAGGCA
    GACATCGCCA CCCAGGGCTG GTTCATTGGG CTCATGTGTG CCATCGCCCT CCTGGTGCTG
    ATCCTGCTCA TCGTCTGCTT CATCAAGAGG AGTCGAGGTG GCAAATACCC AGTGCGAGAA
    AAGAAGGACG TTCCTCTTGG CCCCGAAGAC CCCAAGGAAG AGGATGGCTC CTTTGACTAC
    AGTGACGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACGT CCCTCGATGG TACCATCAAG
    CAGCAAGAGA GCGACGACAG CCTGGTGGAC TATGGCGAGG GTGGCGAGGG GCAGTTCAAT
    GAGGATGGGT CCTTCATCGG CCAGTACACG GTCAAAAAGG ACAAAGAGGA GACAGAGGGC
    AATGAAAGCT CAGAGGCCAC ATCGCCTGTC AACGCCATCT ATTCTCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640839.2
    CDS303..3776
    Translation

    Target ORF information:

    RefSeq Version XM_005640782.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640782.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CTATCCCAGC CCCCAACCAT CACCAAGCAG
    TCAGTGAAGG ACCACATCGT GGATCCCCGT GATAACATCC TGATTGAGTG TGAAGCAAAG
    GGGAACCCTG CCCCCAGCTT CCACTGGACT CGAAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCAATGAG GAGAAAGTCT GGGACCCTGG TGATCGACTT CCGTAGTGGT
    GGGCGGCCCG AAGAATATGA AGGGGAATAC CAGTGCTTTG CCCGCAACAA ATTTGGCACG
    GCCCTGTCCA ATAGGATCCG CCTGCAGGTG TCCAAGTCTC CCCTGTGGCC CAAGGAGAAC
    CTAGACCCAG TCGTGGTTCA AGAAGGTGCT CCTTTGACAC TCCAGTGCAA CCCTCCACCC
    GGACTTCCAT CCCCGGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACTCAGGAC
    AAGCGTGTCT CTCAGGGCCA CAACGGTGAC CTGTATTTCT CCAACGTGAT GCTTCAGGAT
    ATGCAGACAG ACTACAGCTG CAACGCGCGC TTCCACTTCA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCACA CGAGGAGTTG CAGAAAGGAC ACCCAGCTTC
    ATGTATCCCC AGGGCACAGC GAGCAGTCAG ATGGTGCTGC GTGGCACGGA TCTTCTGCTG
    GAGTGCATCG CCTCTGGGGT CCCCACGCCA GACATCGCAT GGTACAAGAA AGGTGGGGAC
    CTCCCATCTG ACAAGGCCAA GTTTGAGAAC TTTAACAAGG CTCTGCGTAT CACCAACGTC
    TCCGAGGAAG ACTCTGGGGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG
    CACACGATCT CGGTGAGAGT AAAGGCTGCT CCCTACTGGC TGGACGAACC CAAGAACCTT
    ATCCTGGCTC CCGGTGAGGA CGGGAGACTG GTGTGTCGAG CCAATGGGAA CCCCAAGCCC
    ACTGTCCAGT GGATGGTAAA TGGGGAACCA CTGCAATCGG CACCACCCAA CCCAAACCGT
    GAGGTGGCTG GGGACACCAT CATCTTCCGG GACACCCAGA TCAGCAGCAG AGCTGTGTAC
    CAGTGCAACA CCTCCAACGA GCATGGCTAC CTACTGGCCA ACGCCTTCGT CAGCGTGCTG
    GACGTGCCAC CCCGGATGCT GTCACCCCGG AACCAGCTCA TTAGGGTGAT CCTGTACAAC
    CGGACACGGC TGGACTGCCC ATTCTTCGGG TCTCCCATCC CCACACTCCG ATGGTTTAAG
    AACGGGCAGG GAAGTAACCT GGATGGTGGT AACTACCACG TCTACGAGAA TGGCAGTCTG
    GAAATCAAGA TGATCCGCAA AGAGGACCAA GGCATCTACA CCTGTGTCGC CACCAACATC
    CTGGGCAAAG CTGAGAACCA GGTCCGCCTG GAGGTCAAAG ACCCCACCAG GATCTACCGG
    ATGCCTGAAG ACCAGGTGGC CAAAAGGGGC ACGACGGTGC AGCTGGAGTG TCGGGTGAAG
    CACGACCCCT CCCTGAAGCT CACGGTCTCC TGGCTGAAGG ATGATGAGCC TCTCTACATA
    GGAAACAGGA TGAAGAAGGA AGACGACTCC CTGACCATCT TTGGTGTGGC TGAGCGGGAC
    CAGGGCAGTT ACACATGTGT TGCCAGCACT GAGCTGGACC AGGACCTGGC CAAGGCCTAC
    CTCACCGTGC TAGGACGGCC AGACCGACCC CGGGACTTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGATAACA ACAGCCCCAT CACAGACTAC
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGCG TCAACTCTGC CATCCTCCAG TTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATCGCCA TCAACGAGGT GGGCAGCAGC CATCCCAGCC TCCCCTCGGA GCGCTACAGG
    ACCAGCGGGG CGCCCCCTGA GTCCAATCCC GCCGACGTAA AGGGAGAAGG GACCAGAAAG
    AACAACATGG AGATCACTTG GACGCCCATG AACGCCACCT CTGCCTTTGG CCCCAACCTG
    CGCTACATCG TCAAGTGGCG GCGGAGAGAA ACTCGAGAGA CGTGGAACAA CGCCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGCCAGACC CCTGTCTACG TGCCCTATGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CTGTCATCGG TTACTCCGGG
    GAAGACTATC CCAGGGCTGC ACCCACTGAC GTTAAAATCC GAGTCCTGAA CAGCACAGCC
    ATCAGTCTCC AGTGGAACCG CGTTTACCCT GACACAGTCC AGGGCCAGCT CAGAGAGTAC
    CGAGCCTACT ACTGGAGGGA GAGCAGCTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA
    CAGCAAGCCA GCTTCCCTGG TGACCGCCCC CGCGGCGTGG TGTCCCGCCT CTTCCCCTAC
    AGTAACTACA AGCTGGAGAT GGTCGTGGTC AATGGGAGAG GCGATGGGCC TCGCAGTGAA
    ACCAAGGAGT TCACCACCCC GGAAGGAGTA CCCAGTGCCC CCAGACGTTT CCGAGTCCGG
    CAGCCCAACC TGGAGACAAT CAACCTGGAA TGGGATCATC CGGAACACCC CAATGGGATC
    CTGATCGGAT ACACTCTCAA ATATGTGGCT TTTAATGGAA CCAAATTAGG AAAGCAGATA
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGATGC AAAGAGCAGA CCCCGTGTCG
    CGCTACCGCT TTACCCTCAG TGCCAGGACG CAGGTGGGCT CTGGGGAAGC AGTCACAGAG
    GAGTCACCAG CACCCCCAAA TGAAGCTACT CCAACAGCAG CTTACACCAA TAACCAGGCA
    GACATCGCCA CCCAGGGCTG GTTCATTGGG CTCATGTGTG CCATCGCCCT CCTGGTGCTG
    ATCCTGCTCA TCGTCTGCTT CATCAAGAGG AGTCGAGGTG GCAAATACCC AGTGCGAGAA
    AAGAAGGACG TTCCTCTTGG CCCCGAAGAC CCCAAGGAAG AGGATGGCTC CTTTGACTAC
    AGTGACGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACGT CCCTCGATGG TACCATCAAG
    CAGCAAGAGA GCGACGACAG CCTGGTGGAC TATGGCGAGG GTGGCGAGGG GCAGTTCAAT
    GAGGATGGGT CCTTCATCGG CCAGTACACG GTCAAAAAGG ACAAAGAGGA GACAGAGGGC
    AATGAAAGCT CAGAGGCCAC ATCGCCTGTC AACGCCATCT ATTCTCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe31640
    Clone ID Related Accession (Same CDS sequence) XM_005640773.2 , XM_005640773.3 , XM_005640783.3 , XM_022415410.1 , XM_005640783.2
    Accession Version XM_005640773.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4083bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neurofascin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640773.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TACTCCAACA GCAGCTCCTC CCACGTTGCC CCCGACCACG
    GTGAGTGTGA CGGGCGTTGT GAGCAGTACA GACGCTACTG CCACCGCTGC CACCACCGAA
    GCCACAACAG TCCCCACCAT CCCAAGTGTC GCACCTACCA CCATGGCCAC CACCACCGTC
    GCCACAACTA CAACCACTGC CGCCACCACC ATGGAGAGGC CTCCCACCAC CACCTCCAAG
    ACTAAGATGC CCGAATCCGC CCCCGACAGG CAGTCCATAT GGAACGTCAC GGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CACCTGGAGA CACAATCTCG GGCCCGGAAC TGCCTTTGTG
    GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CTGTTAAGAG CCAGGTCCAG
    CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG
    GACAAGGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAT
    AACCAGGCAG ACATCGCCAC CCAGGGCTGG TTCATTGGGC TCATGTGTGC CATCGCCCTC
    CTGGTGCTGA TCCTGCTCAT CGTCTGCTTC ATCAAGAGGA GTCGAGGTGG CAAATACCCA
    GTGCGAGAAA AGAAGGACGT TCCTCTTGGC CCCGAAGACC CCAAGGAAGA GGATGGCTCC
    TTTGACTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTCGATGGT
    ACCATCAAGC AGCAAGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGG
    CAGTTCAATG AGGATGGGTC CTTCATCGGC CAGTACACGG TCAAAAAGGA CAAAGAGGAG
    ACAGAGGGCA ATGAAAGCTC AGAGGCCACA TCGCCTGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640830.2
    CDS288..4370
    Translation

    Target ORF information:

    RefSeq Version XM_005640773.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640773.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TACTCCAACA GCAGCTCCTC CCACGTTGCC CCCGACCACG
    GTGAGTGTGA CGGGCGTTGT GAGCAGTACA GACGCTACTG CCACCGCTGC CACCACCGAA
    GCCACAACAG TCCCCACCAT CCCAAGTGTC GCACCTACCA CCATGGCCAC CACCACCGTC
    GCCACAACTA CAACCACTGC CGCCACCACC ATGGAGAGGC CTCCCACCAC CACCTCCAAG
    ACTAAGATGC CCGAATCCGC CCCCGACAGG CAGTCCATAT GGAACGTCAC GGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CACCTGGAGA CACAATCTCG GGCCCGGAAC TGCCTTTGTG
    GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CTGTTAAGAG CCAGGTCCAG
    CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG
    GACAAGGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAT
    AACCAGGCAG ACATCGCCAC CCAGGGCTGG TTCATTGGGC TCATGTGTGC CATCGCCCTC
    CTGGTGCTGA TCCTGCTCAT CGTCTGCTTC ATCAAGAGGA GTCGAGGTGG CAAATACCCA
    GTGCGAGAAA AGAAGGACGT TCCTCTTGGC CCCGAAGACC CCAAGGAAGA GGATGGCTCC
    TTTGACTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTCGATGGT
    ACCATCAAGC AGCAAGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGG
    CAGTTCAATG AGGATGGGTC CTTCATCGGC CAGTACACGG TCAAAAAGGA CAAAGAGGAG
    ACAGAGGGCA ATGAAAGCTC AGAGGCCACA TCGCCTGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe31640
    Clone ID Related Accession (Same CDS sequence) XM_005640773.2 , XM_005640773.3 , XM_005640783.3 , XM_022415410.1 , XM_005640783.2
    Accession Version XM_005640773.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4083bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurofascin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005640773.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TACTCCAACA GCAGCTCCTC CCACGTTGCC CCCGACCACG
    GTGAGTGTGA CGGGCGTTGT GAGCAGTACA GACGCTACTG CCACCGCTGC CACCACCGAA
    GCCACAACAG TCCCCACCAT CCCAAGTGTC GCACCTACCA CCATGGCCAC CACCACCGTC
    GCCACAACTA CAACCACTGC CGCCACCACC ATGGAGAGGC CTCCCACCAC CACCTCCAAG
    ACTAAGATGC CCGAATCCGC CCCCGACAGG CAGTCCATAT GGAACGTCAC GGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CACCTGGAGA CACAATCTCG GGCCCGGAAC TGCCTTTGTG
    GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CTGTTAAGAG CCAGGTCCAG
    CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG
    GACAAGGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAT
    AACCAGGCAG ACATCGCCAC CCAGGGCTGG TTCATTGGGC TCATGTGTGC CATCGCCCTC
    CTGGTGCTGA TCCTGCTCAT CGTCTGCTTC ATCAAGAGGA GTCGAGGTGG CAAATACCCA
    GTGCGAGAAA AGAAGGACGT TCCTCTTGGC CCCGAAGACC CCAAGGAAGA GGATGGCTCC
    TTTGACTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTCGATGGT
    ACCATCAAGC AGCAAGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGG
    CAGTTCAATG AGGATGGGTC CTTCATCGGC CAGTACACGG TCAAAAAGGA CAAAGAGGAG
    ACAGAGGGCA ATGAAAGCTC AGAGGCCACA TCGCCTGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640830.2
    CDS304..4386
    Translation

    Target ORF information:

    RefSeq Version XM_005640773.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640773.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TACTCCAACA GCAGCTCCTC CCACGTTGCC CCCGACCACG
    GTGAGTGTGA CGGGCGTTGT GAGCAGTACA GACGCTACTG CCACCGCTGC CACCACCGAA
    GCCACAACAG TCCCCACCAT CCCAAGTGTC GCACCTACCA CCATGGCCAC CACCACCGTC
    GCCACAACTA CAACCACTGC CGCCACCACC ATGGAGAGGC CTCCCACCAC CACCTCCAAG
    ACTAAGATGC CCGAATCCGC CCCCGACAGG CAGTCCATAT GGAACGTCAC GGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CACCTGGAGA CACAATCTCG GGCCCGGAAC TGCCTTTGTG
    GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CTGTTAAGAG CCAGGTCCAG
    CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG
    GACAAGGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAT
    AACCAGGCAG ACATCGCCAC CCAGGGCTGG TTCATTGGGC TCATGTGTGC CATCGCCCTC
    CTGGTGCTGA TCCTGCTCAT CGTCTGCTTC ATCAAGAGGA GTCGAGGTGG CAAATACCCA
    GTGCGAGAAA AGAAGGACGT TCCTCTTGGC CCCGAAGACC CCAAGGAAGA GGATGGCTCC
    TTTGACTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTCGATGGT
    ACCATCAAGC AGCAAGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGG
    CAGTTCAATG AGGATGGGTC CTTCATCGGC CAGTACACGG TCAAAAAGGA CAAAGAGGAG
    ACAGAGGGCA ATGAAAGCTC AGAGGCCACA TCGCCTGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe31640
    Clone ID Related Accession (Same CDS sequence) XM_005640773.2 , XM_005640773.3 , XM_005640783.3 , XM_022415410.1 , XM_005640783.2
    Accession Version XM_005640783.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4083bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurofascin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005640783.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TACTCCAACA GCAGCTCCTC CCACGTTGCC CCCGACCACG
    GTGAGTGTGA CGGGCGTTGT GAGCAGTACA GACGCTACTG CCACCGCTGC CACCACCGAA
    GCCACAACAG TCCCCACCAT CCCAAGTGTC GCACCTACCA CCATGGCCAC CACCACCGTC
    GCCACAACTA CAACCACTGC CGCCACCACC ATGGAGAGGC CTCCCACCAC CACCTCCAAG
    ACTAAGATGC CCGAATCCGC CCCCGACAGG CAGTCCATAT GGAACGTCAC GGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CACCTGGAGA CACAATCTCG GGCCCGGAAC TGCCTTTGTG
    GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CTGTTAAGAG CCAGGTCCAG
    CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG
    GACAAGGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAT
    AACCAGGCAG ACATCGCCAC CCAGGGCTGG TTCATTGGGC TCATGTGTGC CATCGCCCTC
    CTGGTGCTGA TCCTGCTCAT CGTCTGCTTC ATCAAGAGGA GTCGAGGTGG CAAATACCCA
    GTGCGAGAAA AGAAGGACGT TCCTCTTGGC CCCGAAGACC CCAAGGAAGA GGATGGCTCC
    TTTGACTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTCGATGGT
    ACCATCAAGC AGCAAGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGG
    CAGTTCAATG AGGATGGGTC CTTCATCGGC CAGTACACGG TCAAAAAGGA CAAAGAGGAG
    ACAGAGGGCA ATGAAAGCTC AGAGGCCACA TCGCCTGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640840.1
    CDS497..4579
    Translation

    Target ORF information:

    RefSeq Version XM_005640783.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640783.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TACTCCAACA GCAGCTCCTC CCACGTTGCC CCCGACCACG
    GTGAGTGTGA CGGGCGTTGT GAGCAGTACA GACGCTACTG CCACCGCTGC CACCACCGAA
    GCCACAACAG TCCCCACCAT CCCAAGTGTC GCACCTACCA CCATGGCCAC CACCACCGTC
    GCCACAACTA CAACCACTGC CGCCACCACC ATGGAGAGGC CTCCCACCAC CACCTCCAAG
    ACTAAGATGC CCGAATCCGC CCCCGACAGG CAGTCCATAT GGAACGTCAC GGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CACCTGGAGA CACAATCTCG GGCCCGGAAC TGCCTTTGTG
    GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CTGTTAAGAG CCAGGTCCAG
    CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG
    GACAAGGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAT
    AACCAGGCAG ACATCGCCAC CCAGGGCTGG TTCATTGGGC TCATGTGTGC CATCGCCCTC
    CTGGTGCTGA TCCTGCTCAT CGTCTGCTTC ATCAAGAGGA GTCGAGGTGG CAAATACCCA
    GTGCGAGAAA AGAAGGACGT TCCTCTTGGC CCCGAAGACC CCAAGGAAGA GGATGGCTCC
    TTTGACTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTCGATGGT
    ACCATCAAGC AGCAAGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGG
    CAGTTCAATG AGGATGGGTC CTTCATCGGC CAGTACACGG TCAAAAAGGA CAAAGAGGAG
    ACAGAGGGCA ATGAAAGCTC AGAGGCCACA TCGCCTGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe31640
    Clone ID Related Accession (Same CDS sequence) XM_005640773.2 , XM_005640773.3 , XM_005640783.3 , XM_022415410.1 , XM_005640783.2
    Accession Version XM_022415410.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4083bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurofascin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TACTCCAACA GCAGCTCCTC CCACGTTGCC CCCGACCACG
    GTGAGTGTGA CGGGCGTTGT GAGCAGTACA GACGCTACTG CCACCGCTGC CACCACCGAA
    GCCACAACAG TCCCCACCAT CCCAAGTGTC GCACCTACCA CCATGGCCAC CACCACCGTC
    GCCACAACTA CAACCACTGC CGCCACCACC ATGGAGAGGC CTCCCACCAC CACCTCCAAG
    ACTAAGATGC CCGAATCCGC CCCCGACAGG CAGTCCATAT GGAACGTCAC GGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CACCTGGAGA CACAATCTCG GGCCCGGAAC TGCCTTTGTG
    GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CTGTTAAGAG CCAGGTCCAG
    CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG
    GACAAGGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAT
    AACCAGGCAG ACATCGCCAC CCAGGGCTGG TTCATTGGGC TCATGTGTGC CATCGCCCTC
    CTGGTGCTGA TCCTGCTCAT CGTCTGCTTC ATCAAGAGGA GTCGAGGTGG CAAATACCCA
    GTGCGAGAAA AGAAGGACGT TCCTCTTGGC CCCGAAGACC CCAAGGAAGA GGATGGCTCC
    TTTGACTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTCGATGGT
    ACCATCAAGC AGCAAGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGG
    CAGTTCAATG AGGATGGGTC CTTCATCGGC CAGTACACGG TCAAAAAGGA CAAAGAGGAG
    ACAGAGGGCA ATGAAAGCTC AGAGGCCACA TCGCCTGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022271118.1
    CDS179..4261
    Translation

    Target ORF information:

    RefSeq Version XM_022415410.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022415410.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TACTCCAACA GCAGCTCCTC CCACGTTGCC CCCGACCACG
    GTGAGTGTGA CGGGCGTTGT GAGCAGTACA GACGCTACTG CCACCGCTGC CACCACCGAA
    GCCACAACAG TCCCCACCAT CCCAAGTGTC GCACCTACCA CCATGGCCAC CACCACCGTC
    GCCACAACTA CAACCACTGC CGCCACCACC ATGGAGAGGC CTCCCACCAC CACCTCCAAG
    ACTAAGATGC CCGAATCCGC CCCCGACAGG CAGTCCATAT GGAACGTCAC GGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CACCTGGAGA CACAATCTCG GGCCCGGAAC TGCCTTTGTG
    GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CTGTTAAGAG CCAGGTCCAG
    CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG
    GACAAGGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAT
    AACCAGGCAG ACATCGCCAC CCAGGGCTGG TTCATTGGGC TCATGTGTGC CATCGCCCTC
    CTGGTGCTGA TCCTGCTCAT CGTCTGCTTC ATCAAGAGGA GTCGAGGTGG CAAATACCCA
    GTGCGAGAAA AGAAGGACGT TCCTCTTGGC CCCGAAGACC CCAAGGAAGA GGATGGCTCC
    TTTGACTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTCGATGGT
    ACCATCAAGC AGCAAGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGG
    CAGTTCAATG AGGATGGGTC CTTCATCGGC CAGTACACGG TCAAAAAGGA CAAAGAGGAG
    ACAGAGGGCA ATGAAAGCTC AGAGGCCACA TCGCCTGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe31640
    Clone ID Related Accession (Same CDS sequence) XM_005640773.2 , XM_005640773.3 , XM_005640783.3 , XM_022415410.1 , XM_005640783.2
    Accession Version XM_005640783.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4083bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neurofascin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640783.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TACTCCAACA GCAGCTCCTC CCACGTTGCC CCCGACCACG
    GTGAGTGTGA CGGGCGTTGT GAGCAGTACA GACGCTACTG CCACCGCTGC CACCACCGAA
    GCCACAACAG TCCCCACCAT CCCAAGTGTC GCACCTACCA CCATGGCCAC CACCACCGTC
    GCCACAACTA CAACCACTGC CGCCACCACC ATGGAGAGGC CTCCCACCAC CACCTCCAAG
    ACTAAGATGC CCGAATCCGC CCCCGACAGG CAGTCCATAT GGAACGTCAC GGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CACCTGGAGA CACAATCTCG GGCCCGGAAC TGCCTTTGTG
    GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CTGTTAAGAG CCAGGTCCAG
    CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG
    GACAAGGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAT
    AACCAGGCAG ACATCGCCAC CCAGGGCTGG TTCATTGGGC TCATGTGTGC CATCGCCCTC
    CTGGTGCTGA TCCTGCTCAT CGTCTGCTTC ATCAAGAGGA GTCGAGGTGG CAAATACCCA
    GTGCGAGAAA AGAAGGACGT TCCTCTTGGC CCCGAAGACC CCAAGGAAGA GGATGGCTCC
    TTTGACTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTCGATGGT
    ACCATCAAGC AGCAAGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGG
    CAGTTCAATG AGGATGGGTC CTTCATCGGC CAGTACACGG TCAAAAAGGA CAAAGAGGAG
    ACAGAGGGCA ATGAAAGCTC AGAGGCCACA TCGCCTGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640840.1
    CDS280..4362
    Translation

    Target ORF information:

    RefSeq Version XM_005640783.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neurofascin (NFASC), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640783.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAGGC AGCCGCCGCC GCCCTCGGTC CATGCAGCCA TCCTGCTCTG CCTCCTCAGC 
    CTCAGTGGGG CCATCGAGAT TCCCATGGAT CCAAGCATTC AGAATGAGCT ATCCCAGCCC
    CCAACCATCA CCAAGCAGTC AGTGAAGGAC CACATCGTGG ATCCCCGTGA TAACATCCTG
    ATTGAGTGTG AAGCAAAGGG GAACCCTGCC CCCAGCTTCC ACTGGACTCG AAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCAATGAGGA GAAAGTCTGG GACCCTGGTG
    ATCGACTTCC GTAGTGGTGG GCGGCCCGAA GAATATGAAG GGGAATACCA GTGCTTTGCC
    CGCAACAAAT TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC CAAGTCTCCC
    CTGTGGCCCA AGGAGAACCT AGACCCAGTC GTGGTTCAAG AAGGTGCTCC TTTGACACTC
    CAGTGCAACC CTCCACCCGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CTCAGGACAA GCGTGTCTCT CAGGGCCACA ACGGTGACCT GTATTTCTCC
    AACGTGATGC TTCAGGATAT GCAGACAGAC TACAGCTGCA ACGCGCGCTT CCACTTCACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCAC GAGGAGTTGC AGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACAGCG AGCAGTCAGA TGGTGCTGCG TGGCACGGAT
    CTTCTGCTGG AGTGCATCGC CTCTGGGGTC CCCACGCCAG ACATCGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTGCGTATC
    ACCAACGTCT CCGAGGAAGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC
    AAGAACCTTA TCCTGGCTCC CGGTGAGGAC GGGAGACTGG TGTGTCGAGC CAATGGGAAC
    CCCAAGCCCA CTGTCCAGTG GATGGTAAAT GGGGAACCAC TGCAATCGGC ACCACCCAAC
    CCAAACCGTG AGGTGGCTGG GGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGA
    GCTGTGTACC AGTGCAACAC CTCCAACGAG CATGGCTACC TACTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC CCGGATGCTG TCACCCCGGA ACCAGCTCAT TAGGGTGATC
    CTGTACAACC GGACACGGCT GGACTGCCCA TTCTTCGGGT CTCCCATCCC CACACTCCGA
    TGGTTTAAGA ACGGGCAGGG AAGTAACCTG GATGGTGGTA ACTACCACGT CTACGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCGCAAA GAGGACCAAG GCATCTACAC CTGTGTCGCC
    ACCAACATCC TGGGCAAAGC TGAGAACCAG GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACCGGA TGCCTGAAGA CCAGGTGGCC AAAAGGGGCA CGACGGTGCA GCTGGAGTGT
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACGGTCTCCT GGCTGAAGGA TGATGAGCCT
    CTCTACATAG GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGTGTGGCT
    GAGCGGGACC AGGGCAGTTA CACATGTGTT GCCAGCACTG AGCTGGACCA GGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACCG ACCCCGGGAC TTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTGA CCTGGATCCC AGGGGATGAT AACAACAGCC CCATCACAGA CTACGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGCGTCAACT CTGCCATCCT CCAGTTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATC
    GCCATCAACG AGGTGGGCAG CAGCCATCCC AGCCTCCCCT CGGAGCGCTA CAGGACCAGC
    GGGGCGCCCC CTGAGTCCAA TCCCGCCGAC GTAAAGGGAG AAGGGACCAG AAAGAACAAC
    ATGGAGATCA CTTGGACGCC CATGAACGCC ACCTCTGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTCAAGT GGCGGCGGAG AGAAACTCGA GAGACGTGGA ACAACGCCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGCCA GACCCCTGTC TACGTGCCCT ATGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACTGTCA TCGGTTACTC CGGGGAAGAC
    TATCCCAGGG CTGCACCCAC TGACGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGT
    CTCCAGTGGA ACCGCGTTTA CCCTGACACA GTCCAGGGCC AGCTCAGAGA GTACCGAGCC
    TACTACTGGA GGGAGAGCAG CTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAA
    GCCAGCTTCC CTGGTGACCG CCCCCGCGGC GTGGTGTCCC GCCTCTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTCGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAAACCAAG
    GAGTTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGAC GTTTCCGAGT CCGGCAGCCC
    AACCTGGAGA CAATCAACCT GGAATGGGAT CATCCGGAAC ACCCCAATGG GATCCTGATC
    GGATACACTC TCAAATATGT GGCTTTTAAT GGAACCAAAT TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG ATGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCAGTCAC AGAGGAGTCA
    CCAGCACCCC CAAATGAAGC TACTCCAACA GCAGCTCCTC CCACGTTGCC CCCGACCACG
    GTGAGTGTGA CGGGCGTTGT GAGCAGTACA GACGCTACTG CCACCGCTGC CACCACCGAA
    GCCACAACAG TCCCCACCAT CCCAAGTGTC GCACCTACCA CCATGGCCAC CACCACCGTC
    GCCACAACTA CAACCACTGC CGCCACCACC ATGGAGAGGC CTCCCACCAC CACCTCCAAG
    ACTAAGATGC CCGAATCCGC CCCCGACAGG CAGTCCATAT GGAACGTCAC GGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CACCTGGAGA CACAATCTCG GGCCCGGAAC TGCCTTTGTG
    GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CTGTTAAGAG CCAGGTCCAG
    CCCGTACAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGCGT TTATTCCCGG
    GACAAGGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAT
    AACCAGGCAG ACATCGCCAC CCAGGGCTGG TTCATTGGGC TCATGTGTGC CATCGCCCTC
    CTGGTGCTGA TCCTGCTCAT CGTCTGCTTC ATCAAGAGGA GTCGAGGTGG CAAATACCCA
    GTGCGAGAAA AGAAGGACGT TCCTCTTGGC CCCGAAGACC CCAAGGAAGA GGATGGCTCC
    TTTGACTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACGTC CCTCGATGGT
    ACCATCAAGC AGCAAGAGAG CGACGACAGC CTGGTGGACT ATGGCGAGGG TGGCGAGGGG
    CAGTTCAATG AGGATGGGTC CTTCATCGGC CAGTACACGG TCAAAAAGGA CAAAGAGGAG
    ACAGAGGGCA ATGAAAGCTC AGAGGCCACA TCGCCTGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.