CASR cDNA ORF clone, Canis lupus familiaris(dog)

The following CASR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CASR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe30072 NM_001081509.2
Latest version!
Canis lupus familiaris calcium sensing receptor (CASR), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe30072 XM_022413728.1
Latest version!
Canis lupus familiaris calcium sensing receptor (CASR), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe30072 XM_022413729.1
Latest version!
Canis lupus familiaris calcium sensing receptor (CASR), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe30072 XM_014110143.2
Latest version!
Canis lupus familiaris calcium sensing receptor (CASR), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe30072 XM_014110142.2
Latest version!
Canis lupus familiaris calcium sensing receptor (CASR), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe30072 XM_022413726.1
Latest version!
Canis lupus familiaris calcium sensing receptor (CASR), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe30072 XM_014110141.2
Latest version!
Canis lupus familiaris calcium sensing receptor (CASR), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe30072 XM_022413727.1
Latest version!
Canis lupus familiaris calcium sensing receptor (CASR), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
View Old Accession Versions >>
OCe30072 XM_014110143.1 Canis lupus familiaris calcium-sensing receptor (CASR), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe30072 XM_014110141.1 Canis lupus familiaris calcium-sensing receptor (CASR), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe30072 XM_014110142.1 Canis lupus familiaris calcium-sensing receptor (CASR), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe30072
    Clone ID Related Accession (Same CDS sequence) XM_014110142.1 , NM_001081509.2 , XM_014110141.2 , XM_014110143.2 , XM_022413728.1 , XM_014110143.1 , XM_022413727.1 , XM_022413729.1 , XM_014110141.1 , XM_022413726.1 , XM_014110142.2
    Accession Version NM_001081509.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Canis lupus familiaris(dog)
    Product extracellular calcium-sensing receptor precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03017025.1. On Sep 2, 2015 this sequence version replaced NM_001081509.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: DQ366290.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA4547738 [ECO:0000348] ##Evidence-Data-END##

    1
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    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATTTC ACAGCTGCTC TTTGATCCTC TTGGCAATCA CCTGGTGCAC TTCTGCCTAT 
    GGGCCTGACC AACGAGCCCA GAAGAAAGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
    CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTAAAGTCAA GGCCGGAGTC TGTGGAATGT
    ATCAGGTACA ATTTCCGCGG GTTTCGTTGG TTACAAGCAA TGATATTTGC CATCGAGGAA
    ATAAACAGCA GCCCAGCCCT TCTTCCAAAC ATGACACTGG GATACAGAAT ATTTGACACT
    TGCAACACCG TTTCTAAAGC CTTGGAGGCC ACTCTGAGTT TTGTGGCACA GAATAAAATT
    GATTCTCTGA ACCTTGACGA GTTCTGCAAC TGCTCAGAGC ATATCCCCTC TACTATCGCT
    GTGGTGGGAG CAACTGGCTC GGGCATCTCC ACGGCTGTGG CAAACCTGCT GGGCCTCTTC
    TACATCCCCC AGGTCAGCTA TGCCTCCTCC AGCAGACTCC TCAGCAATAA GAATCAGTTC
    AAGTCCTTCC TCCGTACCAT CCCCAATGAT GAACACCAGG CCACTGCCAT GGCAGACATT
    ATTGAGTATT TCCGCTGGAA CTGGGTGGGC ACCATTGCAG CTGATGATGA CTACGGCCGG
    CCAGGGATTG AGAAGTTCCG AGAGGAAGCA GAGGAGAGGG ACATCTGCAT CGACTTCAGT
    GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATTCAGC AAGTGGTAGA GGTGATCCAG
    AATTCCACAG CCAAAGTCAT TGTTGTTTTC TCCAGTGGCC CAGACCTTGA ACCCCTCATC
    AAGGAGATCG TCCGGCGAAA TATCACAGGA AGGATTTGGC TGGCCAGTGA GGCCTGGGCC
    AGCTCTTCCT TGATTGCCAT GCCCGAGTAC TTCCATGTGG TTGGAGGTAC CATTGGATTC
    GCTTTGAAGG CTGGGCAGAT CCCAGGTTTC CGGGAATTCC TGCAGAAAGT CCATCCCAGA
    AAGTCTGTCC ACAACGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
    CAAGAAGGTG CTAAAGGGCC TTTATCCATG GACACTTTCC TGAGAGGCCA CGAAGAAGGT
    GGTGGCAGGA TAAGCAACAG CTCCACTGCC TTCCGACCTC TTTGCACAGG AGATGAGAAC
    ATCAGTAGTG TGGAGACCCC TTATATGGAT TATACACACT TACGGATATC CTACAACGTC
    TACTTAGCAG TCTATTCCAT TGCTCATGCC CTGCAAGATA TATATACATG CTTACCTGGG
    AGAGGGCTCT TCACCAACGG TTCCTGTGCT GATATTAAGA AGGTTGAGGC TTGGCAGGTC
    TTGAAGCACC TACGGCACCT AAACTTTACC AACAATATGG GGGAGCAAGT GACTTTCGAT
    GAATGTGGTG ACCTGATGGG GAACTATTCC ATCATCAACT GGCACCTCTC TCCAGAGGAT
    GGCTCCATAG TGTTTAAGGA AGTCGGATAT TACAATGTCT ATGCCAAGAA AGGAGAAAGA
    CTCTTCATCA ATGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGAT GCCATTTTCC
    AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGAA TCATTGAGGG GGAGCCTACC
    TGCTGCTTTG AGTGTGTGGA GTGCCCCGAC GGGGAGTACA GTGATGAAAC AGATGCAAGT
    GCCTGTGACA AGTGCCCCGA TGACTTCTGG TCCAATGAAA ACCACACTTC GTGCATTGCC
    AAAGAGATTG AGTTTCTGTC CTGGACAGAG CCCTTTGGGA TTGCACTCAC CCTCTTTGCT
    GTGCTGGGCA TTTTCCTGAC AGCTTTCGTG CTGGGGGTCT TCATCAAGTT CCGTAACACG
    CCCATCGTCA AGGCCACCAA CCGAGAGCTC TCGTACCTCC TCCTCTTCTC CTTGCTGTGC
    TGCTTCTCCA GCTCCCTGTT CTTCATTGGC GAGCCCCAGG ACTGGACCTG CCGCCTGCGC
    CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATATCAT GCATCCTGGT GAAAACCAAC
    CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACAAGCT TCCACCGCAA GTGGTGGGGG
    CTCAACCTGC AGTTCCTGCT GGTCTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
    ATCTGGCTCT ACACGGCGCC TCCCTCCAGC TACCGCAACC ATGAGCTGGA GGACGAGATC
    ATCTTCATCA CATGCCACGA GGGCTCCCTG ATGGCCCTGG GCTTCCTGAT TGGCTACACC
    TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
    TTCAACGAGG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
    TTCATTCCAG CCTACGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTGGA AGTGATCGCC
    ATCCTGGCCG CCAGCTTTGG CCTCCTGGCC TGCATCTTCT TCAACAAGGT GTACATCATC
    CTCTTCAAGC CGTCCCGCAA CACCATCGAG GAGGTGCGCT GCAGCACCGC GGCTCACGCT
    TTCAAGGTCG CGGCCCGCGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCGGC
    AGCCTGGGGG GCTCCACGGG CTCCACGCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGT
    GAAGACCCCT TCCCGCAGCC CGAGAGGCAG AAGCAGCAGC AGCCCCTGGC CCTGACCCAG
    CGGGAGCAGC AGCCGCCGCA GCCCTTGACC TTGCCGCCGC AGCCGCAGCC CAGGTGCAAG
    CAGAAGGTCA TCTTCGGCAG TGGCACCGTC ACCTTCTCGC TGAGCTTTGA CGAGCCGCAG
    AAGAGCGCCG CGGCCCCCCG CAATTCCACG CTGCAGCACT CCCTGGAGGC CCAGCGGAGC
    CCCGAGCCCC CCGCCAGACC CCAGGCGTTA CTGCCGCCGC AGGGCGGAGA CACAGACGCG
    GAGCTGCCGG CCCAGGAGCC GGGCCTGCAG GGCCCCGGGG GTGCGGACCG CCGCCCGGAG
    ATGCGAGACC CCGAAGAGCT GTCCCCAGCC CTGGTGGTGT CCAGCTCACA AAGCTTTGTC
    ATCAGCGGCG GAGGCAGCAC GGTCACGGAA AACATACTGC ATTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001074978.1
    CDS1..3228
    Translation

    Target ORF information:

    RefSeq Version NM_001081509.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris calcium sensing receptor (CASR), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001081509.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATTTC ACAGCTGCTC TTTGATCCTC TTGGCAATCA CCTGGTGCAC TTCTGCCTAT 
    GGGCCTGACC AACGAGCCCA GAAGAAAGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
    CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTAAAGTCAA GGCCGGAGTC TGTGGAATGT
    ATCAGGTACA ATTTCCGCGG GTTTCGTTGG TTACAAGCAA TGATATTTGC CATCGAGGAA
    ATAAACAGCA GCCCAGCCCT TCTTCCAAAC ATGACACTGG GATACAGAAT ATTTGACACT
    TGCAACACCG TTTCTAAAGC CTTGGAGGCC ACTCTGAGTT TTGTGGCACA GAATAAAATT
    GATTCTCTGA ACCTTGACGA GTTCTGCAAC TGCTCAGAGC ATATCCCCTC TACTATCGCT
    GTGGTGGGAG CAACTGGCTC GGGCATCTCC ACGGCTGTGG CAAACCTGCT GGGCCTCTTC
    TACATCCCCC AGGTCAGCTA TGCCTCCTCC AGCAGACTCC TCAGCAATAA GAATCAGTTC
    AAGTCCTTCC TCCGTACCAT CCCCAATGAT GAACACCAGG CCACTGCCAT GGCAGACATT
    ATTGAGTATT TCCGCTGGAA CTGGGTGGGC ACCATTGCAG CTGATGATGA CTACGGCCGG
    CCAGGGATTG AGAAGTTCCG AGAGGAAGCA GAGGAGAGGG ACATCTGCAT CGACTTCAGT
    GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATTCAGC AAGTGGTAGA GGTGATCCAG
    AATTCCACAG CCAAAGTCAT TGTTGTTTTC TCCAGTGGCC CAGACCTTGA ACCCCTCATC
    AAGGAGATCG TCCGGCGAAA TATCACAGGA AGGATTTGGC TGGCCAGTGA GGCCTGGGCC
    AGCTCTTCCT TGATTGCCAT GCCCGAGTAC TTCCATGTGG TTGGAGGTAC CATTGGATTC
    GCTTTGAAGG CTGGGCAGAT CCCAGGTTTC CGGGAATTCC TGCAGAAAGT CCATCCCAGA
    AAGTCTGTCC ACAACGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
    CAAGAAGGTG CTAAAGGGCC TTTATCCATG GACACTTTCC TGAGAGGCCA CGAAGAAGGT
    GGTGGCAGGA TAAGCAACAG CTCCACTGCC TTCCGACCTC TTTGCACAGG AGATGAGAAC
    ATCAGTAGTG TGGAGACCCC TTATATGGAT TATACACACT TACGGATATC CTACAACGTC
    TACTTAGCAG TCTATTCCAT TGCTCATGCC CTGCAAGATA TATATACATG CTTACCTGGG
    AGAGGGCTCT TCACCAACGG TTCCTGTGCT GATATTAAGA AGGTTGAGGC TTGGCAGGTC
    TTGAAGCACC TACGGCACCT AAACTTTACC AACAATATGG GGGAGCAAGT GACTTTCGAT
    GAATGTGGTG ACCTGATGGG GAACTATTCC ATCATCAACT GGCACCTCTC TCCAGAGGAT
    GGCTCCATAG TGTTTAAGGA AGTCGGATAT TACAATGTCT ATGCCAAGAA AGGAGAAAGA
    CTCTTCATCA ATGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGAT GCCATTTTCC
    AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGAA TCATTGAGGG GGAGCCTACC
    TGCTGCTTTG AGTGTGTGGA GTGCCCCGAC GGGGAGTACA GTGATGAAAC AGATGCAAGT
    GCCTGTGACA AGTGCCCCGA TGACTTCTGG TCCAATGAAA ACCACACTTC GTGCATTGCC
    AAAGAGATTG AGTTTCTGTC CTGGACAGAG CCCTTTGGGA TTGCACTCAC CCTCTTTGCT
    GTGCTGGGCA TTTTCCTGAC AGCTTTCGTG CTGGGGGTCT TCATCAAGTT CCGTAACACG
    CCCATCGTCA AGGCCACCAA CCGAGAGCTC TCGTACCTCC TCCTCTTCTC CTTGCTGTGC
    TGCTTCTCCA GCTCCCTGTT CTTCATTGGC GAGCCCCAGG ACTGGACCTG CCGCCTGCGC
    CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATATCAT GCATCCTGGT GAAAACCAAC
    CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACAAGCT TCCACCGCAA GTGGTGGGGG
    CTCAACCTGC AGTTCCTGCT GGTCTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
    ATCTGGCTCT ACACGGCGCC TCCCTCCAGC TACCGCAACC ATGAGCTGGA GGACGAGATC
    ATCTTCATCA CATGCCACGA GGGCTCCCTG ATGGCCCTGG GCTTCCTGAT TGGCTACACC
    TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
    TTCAACGAGG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
    TTCATTCCAG CCTACGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTGGA AGTGATCGCC
    ATCCTGGCCG CCAGCTTTGG CCTCCTGGCC TGCATCTTCT TCAACAAGGT GTACATCATC
    CTCTTCAAGC CGTCCCGCAA CACCATCGAG GAGGTGCGCT GCAGCACCGC GGCTCACGCT
    TTCAAGGTCG CGGCCCGCGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCGGC
    AGCCTGGGGG GCTCCACGGG CTCCACGCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGT
    GAAGACCCCT TCCCGCAGCC CGAGAGGCAG AAGCAGCAGC AGCCCCTGGC CCTGACCCAG
    CGGGAGCAGC AGCCGCCGCA GCCCTTGACC TTGCCGCCGC AGCCGCAGCC CAGGTGCAAG
    CAGAAGGTCA TCTTCGGCAG TGGCACCGTC ACCTTCTCGC TGAGCTTTGA CGAGCCGCAG
    AAGAGCGCCG CGGCCCCCCG CAATTCCACG CTGCAGCACT CCCTGGAGGC CCAGCGGAGC
    CCCGAGCCCC CCGCCAGACC CCAGGCGTTA CTGCCGCCGC AGGGCGGAGA CACAGACGCG
    GAGCTGCCGG CCCAGGAGCC GGGCCTGCAG GGCCCCGGGG GTGCGGACCG CCGCCCGGAG
    ATGCGAGACC CCGAAGAGCT GTCCCCAGCC CTGGTGGTGT CCAGCTCACA AAGCTTTGTC
    ATCAGCGGCG GAGGCAGCAC GGTCACGGAA AACATACTGC ATTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30072
    Clone ID Related Accession (Same CDS sequence) XM_014110142.1 , NM_001081509.2 , XM_014110141.2 , XM_014110143.2 , XM_022413728.1 , XM_014110143.1 , XM_022413727.1 , XM_022413729.1 , XM_014110141.1 , XM_022413726.1 , XM_014110142.2
    Accession Version XM_014110142.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product extracellular calcium-sensing receptor isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726118.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATTTC ACAGCTGCTC TTTGATCCTC TTGGCAATCA CCTGGTGCAC TTCTGCCTAT 
    GGGCCTGACC AACGAGCCCA GAAGAAAGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
    CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTAAAGTCAA GGCCGGAGTC TGTGGAATGT
    ATCAGGTACA ATTTCCGCGG GTTTCGTTGG TTACAAGCAA TGATATTTGC CATCGAGGAA
    ATAAACAGCA GCCCAGCCCT TCTTCCAAAC ATGACACTGG GATACAGAAT ATTTGACACT
    TGCAACACCG TTTCTAAAGC CTTGGAGGCC ACTCTGAGTT TTGTGGCACA GAATAAAATT
    GATTCTCTGA ACCTTGACGA GTTCTGCAAC TGCTCAGAGC ATATCCCCTC TACTATCGCT
    GTGGTGGGAG CAACTGGCTC GGGCATCTCC ACGGCTGTGG CAAACCTGCT GGGCCTCTTC
    TACATCCCCC AGGTCAGCTA TGCCTCCTCC AGCAGACTCC TCAGCAATAA GAATCAGTTC
    AAGTCCTTCC TCCGTACCAT CCCCAATGAT GAACACCAGG CCACTGCCAT GGCAGACATT
    ATTGAGTATT TCCGCTGGAA CTGGGTGGGC ACCATTGCAG CTGATGATGA CTACGGCCGG
    CCAGGGATTG AGAAGTTCCG AGAGGAAGCA GAGGAGAGGG ACATCTGCAT CGACTTCAGT
    GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATTCAGC AAGTGGTAGA GGTGATCCAG
    AATTCCACAG CCAAAGTCAT TGTTGTTTTC TCCAGTGGCC CAGACCTTGA ACCCCTCATC
    AAGGAGATCG TCCGGCGAAA TATCACAGGA AGGATTTGGC TGGCCAGTGA GGCCTGGGCC
    AGCTCTTCCT TGATTGCCAT GCCCGAGTAC TTCCATGTGG TTGGAGGTAC CATTGGATTC
    GCTTTGAAGG CTGGGCAGAT CCCAGGTTTC CGGGAATTCC TGCAGAAAGT CCATCCCAGA
    AAGTCTGTCC ACAACGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
    CAAGAAGGTG CTAAAGGGCC TTTATCCATG GACACTTTCC TGAGAGGCCA CGAAGAAGGT
    GGTGGCAGGA TAAGCAACAG CTCCACTGCC TTCCGACCTC TTTGCACAGG AGATGAGAAC
    ATCAGTAGTG TGGAGACCCC TTATATGGAT TATACACACT TACGGATATC CTACAACGTC
    TACTTAGCAG TCTATTCCAT TGCTCATGCC CTGCAAGATA TATATACATG CTTACCTGGG
    AGAGGGCTCT TCACCAACGG TTCCTGTGCT GATATTAAGA AGGTTGAGGC TTGGCAGGTC
    TTGAAGCACC TACGGCACCT AAACTTTACC AACAATATGG GGGAGCAAGT GACTTTCGAT
    GAATGTGGTG ACCTGATGGG GAACTATTCC ATCATCAACT GGCACCTCTC TCCAGAGGAT
    GGCTCCATAG TGTTTAAGGA AGTCGGATAT TACAATGTCT ATGCCAAGAA AGGAGAAAGA
    CTCTTCATCA ATGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGAT GCCATTTTCC
    AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGAA TCATTGAGGG GGAGCCTACC
    TGCTGCTTTG AGTGTGTGGA GTGCCCCGAC GGGGAGTACA GTGATGAAAC AGATGCAAGT
    GCCTGTGACA AGTGCCCCGA TGACTTCTGG TCCAATGAAA ACCACACTTC GTGCATTGCC
    AAAGAGATTG AGTTTCTGTC CTGGACAGAG CCCTTTGGGA TTGCACTCAC CCTCTTTGCT
    GTGCTGGGCA TTTTCCTGAC AGCTTTCGTG CTGGGGGTCT TCATCAAGTT CCGTAACACG
    CCCATCGTCA AGGCCACCAA CCGAGAGCTC TCGTACCTCC TCCTCTTCTC CTTGCTGTGC
    TGCTTCTCCA GCTCCCTGTT CTTCATTGGC GAGCCCCAGG ACTGGACCTG CCGCCTGCGC
    CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATATCAT GCATCCTGGT GAAAACCAAC
    CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACAAGCT TCCACCGCAA GTGGTGGGGG
    CTCAACCTGC AGTTCCTGCT GGTCTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
    ATCTGGCTCT ACACGGCGCC TCCCTCCAGC TACCGCAACC ATGAGCTGGA GGACGAGATC
    ATCTTCATCA CATGCCACGA GGGCTCCCTG ATGGCCCTGG GCTTCCTGAT TGGCTACACC
    TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
    TTCAACGAGG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
    TTCATTCCAG CCTACGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTGGA AGTGATCGCC
    ATCCTGGCCG CCAGCTTTGG CCTCCTGGCC TGCATCTTCT TCAACAAGGT GTACATCATC
    CTCTTCAAGC CGTCCCGCAA CACCATCGAG GAGGTGCGCT GCAGCACCGC GGCTCACGCT
    TTCAAGGTCG CGGCCCGCGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCGGC
    AGCCTGGGGG GCTCCACGGG CTCCACGCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGT
    GAAGACCCCT TCCCGCAGCC CGAGAGGCAG AAGCAGCAGC AGCCCCTGGC CCTGACCCAG
    CGGGAGCAGC AGCCGCCGCA GCCCTTGACC TTGCCGCCGC AGCCGCAGCC CAGGTGCAAG
    CAGAAGGTCA TCTTCGGCAG TGGCACCGTC ACCTTCTCGC TGAGCTTTGA CGAGCCGCAG
    AAGAGCGCCG CGGCCCCCCG CAATTCCACG CTGCAGCACT CCCTGGAGGC CCAGCGGAGC
    CCCGAGCCCC CCGCCAGACC CCAGGCGTTA CTGCCGCCGC AGGGCGGAGA CACAGACGCG
    GAGCTGCCGG CCCAGGAGCC GGGCCTGCAG GGCCCCGGGG GTGCGGACCG CCGCCCGGAG
    ATGCGAGACC CCGAAGAGCT GTCCCCAGCC CTGGTGGTGT CCAGCTCACA AAGCTTTGTC
    ATCAGCGGCG GAGGCAGCAC GGTCACGGAA AACATACTGC ATTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965617.1
    CDS477..3704
    Translation

    Target ORF information:

    RefSeq Version XM_014110142.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris calcium-sensing receptor (CASR), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110142.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATTTC ACAGCTGCTC TTTGATCCTC TTGGCAATCA CCTGGTGCAC TTCTGCCTAT 
    GGGCCTGACC AACGAGCCCA GAAGAAAGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
    CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTAAAGTCAA GGCCGGAGTC TGTGGAATGT
    ATCAGGTACA ATTTCCGCGG GTTTCGTTGG TTACAAGCAA TGATATTTGC CATCGAGGAA
    ATAAACAGCA GCCCAGCCCT TCTTCCAAAC ATGACACTGG GATACAGAAT ATTTGACACT
    TGCAACACCG TTTCTAAAGC CTTGGAGGCC ACTCTGAGTT TTGTGGCACA GAATAAAATT
    GATTCTCTGA ACCTTGACGA GTTCTGCAAC TGCTCAGAGC ATATCCCCTC TACTATCGCT
    GTGGTGGGAG CAACTGGCTC GGGCATCTCC ACGGCTGTGG CAAACCTGCT GGGCCTCTTC
    TACATCCCCC AGGTCAGCTA TGCCTCCTCC AGCAGACTCC TCAGCAATAA GAATCAGTTC
    AAGTCCTTCC TCCGTACCAT CCCCAATGAT GAACACCAGG CCACTGCCAT GGCAGACATT
    ATTGAGTATT TCCGCTGGAA CTGGGTGGGC ACCATTGCAG CTGATGATGA CTACGGCCGG
    CCAGGGATTG AGAAGTTCCG AGAGGAAGCA GAGGAGAGGG ACATCTGCAT CGACTTCAGT
    GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATTCAGC AAGTGGTAGA GGTGATCCAG
    AATTCCACAG CCAAAGTCAT TGTTGTTTTC TCCAGTGGCC CAGACCTTGA ACCCCTCATC
    AAGGAGATCG TCCGGCGAAA TATCACAGGA AGGATTTGGC TGGCCAGTGA GGCCTGGGCC
    AGCTCTTCCT TGATTGCCAT GCCCGAGTAC TTCCATGTGG TTGGAGGTAC CATTGGATTC
    GCTTTGAAGG CTGGGCAGAT CCCAGGTTTC CGGGAATTCC TGCAGAAAGT CCATCCCAGA
    AAGTCTGTCC ACAACGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
    CAAGAAGGTG CTAAAGGGCC TTTATCCATG GACACTTTCC TGAGAGGCCA CGAAGAAGGT
    GGTGGCAGGA TAAGCAACAG CTCCACTGCC TTCCGACCTC TTTGCACAGG AGATGAGAAC
    ATCAGTAGTG TGGAGACCCC TTATATGGAT TATACACACT TACGGATATC CTACAACGTC
    TACTTAGCAG TCTATTCCAT TGCTCATGCC CTGCAAGATA TATATACATG CTTACCTGGG
    AGAGGGCTCT TCACCAACGG TTCCTGTGCT GATATTAAGA AGGTTGAGGC TTGGCAGGTC
    TTGAAGCACC TACGGCACCT AAACTTTACC AACAATATGG GGGAGCAAGT GACTTTCGAT
    GAATGTGGTG ACCTGATGGG GAACTATTCC ATCATCAACT GGCACCTCTC TCCAGAGGAT
    GGCTCCATAG TGTTTAAGGA AGTCGGATAT TACAATGTCT ATGCCAAGAA AGGAGAAAGA
    CTCTTCATCA ATGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGAT GCCATTTTCC
    AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGAA TCATTGAGGG GGAGCCTACC
    TGCTGCTTTG AGTGTGTGGA GTGCCCCGAC GGGGAGTACA GTGATGAAAC AGATGCAAGT
    GCCTGTGACA AGTGCCCCGA TGACTTCTGG TCCAATGAAA ACCACACTTC GTGCATTGCC
    AAAGAGATTG AGTTTCTGTC CTGGACAGAG CCCTTTGGGA TTGCACTCAC CCTCTTTGCT
    GTGCTGGGCA TTTTCCTGAC AGCTTTCGTG CTGGGGGTCT TCATCAAGTT CCGTAACACG
    CCCATCGTCA AGGCCACCAA CCGAGAGCTC TCGTACCTCC TCCTCTTCTC CTTGCTGTGC
    TGCTTCTCCA GCTCCCTGTT CTTCATTGGC GAGCCCCAGG ACTGGACCTG CCGCCTGCGC
    CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATATCAT GCATCCTGGT GAAAACCAAC
    CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACAAGCT TCCACCGCAA GTGGTGGGGG
    CTCAACCTGC AGTTCCTGCT GGTCTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
    ATCTGGCTCT ACACGGCGCC TCCCTCCAGC TACCGCAACC ATGAGCTGGA GGACGAGATC
    ATCTTCATCA CATGCCACGA GGGCTCCCTG ATGGCCCTGG GCTTCCTGAT TGGCTACACC
    TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
    TTCAACGAGG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
    TTCATTCCAG CCTACGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTGGA AGTGATCGCC
    ATCCTGGCCG CCAGCTTTGG CCTCCTGGCC TGCATCTTCT TCAACAAGGT GTACATCATC
    CTCTTCAAGC CGTCCCGCAA CACCATCGAG GAGGTGCGCT GCAGCACCGC GGCTCACGCT
    TTCAAGGTCG CGGCCCGCGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCGGC
    AGCCTGGGGG GCTCCACGGG CTCCACGCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGT
    GAAGACCCCT TCCCGCAGCC CGAGAGGCAG AAGCAGCAGC AGCCCCTGGC CCTGACCCAG
    CGGGAGCAGC AGCCGCCGCA GCCCTTGACC TTGCCGCCGC AGCCGCAGCC CAGGTGCAAG
    CAGAAGGTCA TCTTCGGCAG TGGCACCGTC ACCTTCTCGC TGAGCTTTGA CGAGCCGCAG
    AAGAGCGCCG CGGCCCCCCG CAATTCCACG CTGCAGCACT CCCTGGAGGC CCAGCGGAGC
    CCCGAGCCCC CCGCCAGACC CCAGGCGTTA CTGCCGCCGC AGGGCGGAGA CACAGACGCG
    GAGCTGCCGG CCCAGGAGCC GGGCCTGCAG GGCCCCGGGG GTGCGGACCG CCGCCCGGAG
    ATGCGAGACC CCGAAGAGCT GTCCCCAGCC CTGGTGGTGT CCAGCTCACA AAGCTTTGTC
    ATCAGCGGCG GAGGCAGCAC GGTCACGGAA AACATACTGC ATTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30072
    Clone ID Related Accession (Same CDS sequence) XM_014110142.1 , NM_001081509.2 , XM_014110141.2 , XM_014110143.2 , XM_022413728.1 , XM_014110143.1 , XM_022413727.1 , XM_022413729.1 , XM_014110141.1 , XM_022413726.1 , XM_014110142.2
    Accession Version XM_014110141.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product extracellular calcium-sensing receptor isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006615.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014110141.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATTTC ACAGCTGCTC TTTGATCCTC TTGGCAATCA CCTGGTGCAC TTCTGCCTAT 
    GGGCCTGACC AACGAGCCCA GAAGAAAGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
    CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTAAAGTCAA GGCCGGAGTC TGTGGAATGT
    ATCAGGTACA ATTTCCGCGG GTTTCGTTGG TTACAAGCAA TGATATTTGC CATCGAGGAA
    ATAAACAGCA GCCCAGCCCT TCTTCCAAAC ATGACACTGG GATACAGAAT ATTTGACACT
    TGCAACACCG TTTCTAAAGC CTTGGAGGCC ACTCTGAGTT TTGTGGCACA GAATAAAATT
    GATTCTCTGA ACCTTGACGA GTTCTGCAAC TGCTCAGAGC ATATCCCCTC TACTATCGCT
    GTGGTGGGAG CAACTGGCTC GGGCATCTCC ACGGCTGTGG CAAACCTGCT GGGCCTCTTC
    TACATCCCCC AGGTCAGCTA TGCCTCCTCC AGCAGACTCC TCAGCAATAA GAATCAGTTC
    AAGTCCTTCC TCCGTACCAT CCCCAATGAT GAACACCAGG CCACTGCCAT GGCAGACATT
    ATTGAGTATT TCCGCTGGAA CTGGGTGGGC ACCATTGCAG CTGATGATGA CTACGGCCGG
    CCAGGGATTG AGAAGTTCCG AGAGGAAGCA GAGGAGAGGG ACATCTGCAT CGACTTCAGT
    GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATTCAGC AAGTGGTAGA GGTGATCCAG
    AATTCCACAG CCAAAGTCAT TGTTGTTTTC TCCAGTGGCC CAGACCTTGA ACCCCTCATC
    AAGGAGATCG TCCGGCGAAA TATCACAGGA AGGATTTGGC TGGCCAGTGA GGCCTGGGCC
    AGCTCTTCCT TGATTGCCAT GCCCGAGTAC TTCCATGTGG TTGGAGGTAC CATTGGATTC
    GCTTTGAAGG CTGGGCAGAT CCCAGGTTTC CGGGAATTCC TGCAGAAAGT CCATCCCAGA
    AAGTCTGTCC ACAACGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
    CAAGAAGGTG CTAAAGGGCC TTTATCCATG GACACTTTCC TGAGAGGCCA CGAAGAAGGT
    GGTGGCAGGA TAAGCAACAG CTCCACTGCC TTCCGACCTC TTTGCACAGG AGATGAGAAC
    ATCAGTAGTG TGGAGACCCC TTATATGGAT TATACACACT TACGGATATC CTACAACGTC
    TACTTAGCAG TCTATTCCAT TGCTCATGCC CTGCAAGATA TATATACATG CTTACCTGGG
    AGAGGGCTCT TCACCAACGG TTCCTGTGCT GATATTAAGA AGGTTGAGGC TTGGCAGGTC
    TTGAAGCACC TACGGCACCT AAACTTTACC AACAATATGG GGGAGCAAGT GACTTTCGAT
    GAATGTGGTG ACCTGATGGG GAACTATTCC ATCATCAACT GGCACCTCTC TCCAGAGGAT
    GGCTCCATAG TGTTTAAGGA AGTCGGATAT TACAATGTCT ATGCCAAGAA AGGAGAAAGA
    CTCTTCATCA ATGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGAT GCCATTTTCC
    AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGAA TCATTGAGGG GGAGCCTACC
    TGCTGCTTTG AGTGTGTGGA GTGCCCCGAC GGGGAGTACA GTGATGAAAC AGATGCAAGT
    GCCTGTGACA AGTGCCCCGA TGACTTCTGG TCCAATGAAA ACCACACTTC GTGCATTGCC
    AAAGAGATTG AGTTTCTGTC CTGGACAGAG CCCTTTGGGA TTGCACTCAC CCTCTTTGCT
    GTGCTGGGCA TTTTCCTGAC AGCTTTCGTG CTGGGGGTCT TCATCAAGTT CCGTAACACG
    CCCATCGTCA AGGCCACCAA CCGAGAGCTC TCGTACCTCC TCCTCTTCTC CTTGCTGTGC
    TGCTTCTCCA GCTCCCTGTT CTTCATTGGC GAGCCCCAGG ACTGGACCTG CCGCCTGCGC
    CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATATCAT GCATCCTGGT GAAAACCAAC
    CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACAAGCT TCCACCGCAA GTGGTGGGGG
    CTCAACCTGC AGTTCCTGCT GGTCTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
    ATCTGGCTCT ACACGGCGCC TCCCTCCAGC TACCGCAACC ATGAGCTGGA GGACGAGATC
    ATCTTCATCA CATGCCACGA GGGCTCCCTG ATGGCCCTGG GCTTCCTGAT TGGCTACACC
    TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
    TTCAACGAGG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
    TTCATTCCAG CCTACGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTGGA AGTGATCGCC
    ATCCTGGCCG CCAGCTTTGG CCTCCTGGCC TGCATCTTCT TCAACAAGGT GTACATCATC
    CTCTTCAAGC CGTCCCGCAA CACCATCGAG GAGGTGCGCT GCAGCACCGC GGCTCACGCT
    TTCAAGGTCG CGGCCCGCGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCGGC
    AGCCTGGGGG GCTCCACGGG CTCCACGCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGT
    GAAGACCCCT TCCCGCAGCC CGAGAGGCAG AAGCAGCAGC AGCCCCTGGC CCTGACCCAG
    CGGGAGCAGC AGCCGCCGCA GCCCTTGACC TTGCCGCCGC AGCCGCAGCC CAGGTGCAAG
    CAGAAGGTCA TCTTCGGCAG TGGCACCGTC ACCTTCTCGC TGAGCTTTGA CGAGCCGCAG
    AAGAGCGCCG CGGCCCCCCG CAATTCCACG CTGCAGCACT CCCTGGAGGC CCAGCGGAGC
    CCCGAGCCCC CCGCCAGACC CCAGGCGTTA CTGCCGCCGC AGGGCGGAGA CACAGACGCG
    GAGCTGCCGG CCCAGGAGCC GGGCCTGCAG GGCCCCGGGG GTGCGGACCG CCGCCCGGAG
    ATGCGAGACC CCGAAGAGCT GTCCCCAGCC CTGGTGGTGT CCAGCTCACA AAGCTTTGTC
    ATCAGCGGCG GAGGCAGCAC GGTCACGGAA AACATACTGC ATTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965616.1
    CDS1160..4387
    Translation

    Target ORF information:

    RefSeq Version XM_014110141.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris calcium sensing receptor (CASR), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110141.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATTTC ACAGCTGCTC TTTGATCCTC TTGGCAATCA CCTGGTGCAC TTCTGCCTAT 
    GGGCCTGACC AACGAGCCCA GAAGAAAGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
    CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTAAAGTCAA GGCCGGAGTC TGTGGAATGT
    ATCAGGTACA ATTTCCGCGG GTTTCGTTGG TTACAAGCAA TGATATTTGC CATCGAGGAA
    ATAAACAGCA GCCCAGCCCT TCTTCCAAAC ATGACACTGG GATACAGAAT ATTTGACACT
    TGCAACACCG TTTCTAAAGC CTTGGAGGCC ACTCTGAGTT TTGTGGCACA GAATAAAATT
    GATTCTCTGA ACCTTGACGA GTTCTGCAAC TGCTCAGAGC ATATCCCCTC TACTATCGCT
    GTGGTGGGAG CAACTGGCTC GGGCATCTCC ACGGCTGTGG CAAACCTGCT GGGCCTCTTC
    TACATCCCCC AGGTCAGCTA TGCCTCCTCC AGCAGACTCC TCAGCAATAA GAATCAGTTC
    AAGTCCTTCC TCCGTACCAT CCCCAATGAT GAACACCAGG CCACTGCCAT GGCAGACATT
    ATTGAGTATT TCCGCTGGAA CTGGGTGGGC ACCATTGCAG CTGATGATGA CTACGGCCGG
    CCAGGGATTG AGAAGTTCCG AGAGGAAGCA GAGGAGAGGG ACATCTGCAT CGACTTCAGT
    GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATTCAGC AAGTGGTAGA GGTGATCCAG
    AATTCCACAG CCAAAGTCAT TGTTGTTTTC TCCAGTGGCC CAGACCTTGA ACCCCTCATC
    AAGGAGATCG TCCGGCGAAA TATCACAGGA AGGATTTGGC TGGCCAGTGA GGCCTGGGCC
    AGCTCTTCCT TGATTGCCAT GCCCGAGTAC TTCCATGTGG TTGGAGGTAC CATTGGATTC
    GCTTTGAAGG CTGGGCAGAT CCCAGGTTTC CGGGAATTCC TGCAGAAAGT CCATCCCAGA
    AAGTCTGTCC ACAACGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
    CAAGAAGGTG CTAAAGGGCC TTTATCCATG GACACTTTCC TGAGAGGCCA CGAAGAAGGT
    GGTGGCAGGA TAAGCAACAG CTCCACTGCC TTCCGACCTC TTTGCACAGG AGATGAGAAC
    ATCAGTAGTG TGGAGACCCC TTATATGGAT TATACACACT TACGGATATC CTACAACGTC
    TACTTAGCAG TCTATTCCAT TGCTCATGCC CTGCAAGATA TATATACATG CTTACCTGGG
    AGAGGGCTCT TCACCAACGG TTCCTGTGCT GATATTAAGA AGGTTGAGGC TTGGCAGGTC
    TTGAAGCACC TACGGCACCT AAACTTTACC AACAATATGG GGGAGCAAGT GACTTTCGAT
    GAATGTGGTG ACCTGATGGG GAACTATTCC ATCATCAACT GGCACCTCTC TCCAGAGGAT
    GGCTCCATAG TGTTTAAGGA AGTCGGATAT TACAATGTCT ATGCCAAGAA AGGAGAAAGA
    CTCTTCATCA ATGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGAT GCCATTTTCC
    AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGAA TCATTGAGGG GGAGCCTACC
    TGCTGCTTTG AGTGTGTGGA GTGCCCCGAC GGGGAGTACA GTGATGAAAC AGATGCAAGT
    GCCTGTGACA AGTGCCCCGA TGACTTCTGG TCCAATGAAA ACCACACTTC GTGCATTGCC
    AAAGAGATTG AGTTTCTGTC CTGGACAGAG CCCTTTGGGA TTGCACTCAC CCTCTTTGCT
    GTGCTGGGCA TTTTCCTGAC AGCTTTCGTG CTGGGGGTCT TCATCAAGTT CCGTAACACG
    CCCATCGTCA AGGCCACCAA CCGAGAGCTC TCGTACCTCC TCCTCTTCTC CTTGCTGTGC
    TGCTTCTCCA GCTCCCTGTT CTTCATTGGC GAGCCCCAGG ACTGGACCTG CCGCCTGCGC
    CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATATCAT GCATCCTGGT GAAAACCAAC
    CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACAAGCT TCCACCGCAA GTGGTGGGGG
    CTCAACCTGC AGTTCCTGCT GGTCTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
    ATCTGGCTCT ACACGGCGCC TCCCTCCAGC TACCGCAACC ATGAGCTGGA GGACGAGATC
    ATCTTCATCA CATGCCACGA GGGCTCCCTG ATGGCCCTGG GCTTCCTGAT TGGCTACACC
    TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
    TTCAACGAGG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
    TTCATTCCAG CCTACGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTGGA AGTGATCGCC
    ATCCTGGCCG CCAGCTTTGG CCTCCTGGCC TGCATCTTCT TCAACAAGGT GTACATCATC
    CTCTTCAAGC CGTCCCGCAA CACCATCGAG GAGGTGCGCT GCAGCACCGC GGCTCACGCT
    TTCAAGGTCG CGGCCCGCGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCGGC
    AGCCTGGGGG GCTCCACGGG CTCCACGCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGT
    GAAGACCCCT TCCCGCAGCC CGAGAGGCAG AAGCAGCAGC AGCCCCTGGC CCTGACCCAG
    CGGGAGCAGC AGCCGCCGCA GCCCTTGACC TTGCCGCCGC AGCCGCAGCC CAGGTGCAAG
    CAGAAGGTCA TCTTCGGCAG TGGCACCGTC ACCTTCTCGC TGAGCTTTGA CGAGCCGCAG
    AAGAGCGCCG CGGCCCCCCG CAATTCCACG CTGCAGCACT CCCTGGAGGC CCAGCGGAGC
    CCCGAGCCCC CCGCCAGACC CCAGGCGTTA CTGCCGCCGC AGGGCGGAGA CACAGACGCG
    GAGCTGCCGG CCCAGGAGCC GGGCCTGCAG GGCCCCGGGG GTGCGGACCG CCGCCCGGAG
    ATGCGAGACC CCGAAGAGCT GTCCCCAGCC CTGGTGGTGT CCAGCTCACA AAGCTTTGTC
    ATCAGCGGCG GAGGCAGCAC GGTCACGGAA AACATACTGC ATTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30072
    Clone ID Related Accession (Same CDS sequence) XM_014110142.1 , NM_001081509.2 , XM_014110141.2 , XM_014110143.2 , XM_022413728.1 , XM_014110143.1 , XM_022413727.1 , XM_022413729.1 , XM_014110141.1 , XM_022413726.1 , XM_014110142.2
    Accession Version XM_014110143.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product extracellular calcium-sensing receptor isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006615.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014110143.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATTTC ACAGCTGCTC TTTGATCCTC TTGGCAATCA CCTGGTGCAC TTCTGCCTAT 
    GGGCCTGACC AACGAGCCCA GAAGAAAGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
    CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTAAAGTCAA GGCCGGAGTC TGTGGAATGT
    ATCAGGTACA ATTTCCGCGG GTTTCGTTGG TTACAAGCAA TGATATTTGC CATCGAGGAA
    ATAAACAGCA GCCCAGCCCT TCTTCCAAAC ATGACACTGG GATACAGAAT ATTTGACACT
    TGCAACACCG TTTCTAAAGC CTTGGAGGCC ACTCTGAGTT TTGTGGCACA GAATAAAATT
    GATTCTCTGA ACCTTGACGA GTTCTGCAAC TGCTCAGAGC ATATCCCCTC TACTATCGCT
    GTGGTGGGAG CAACTGGCTC GGGCATCTCC ACGGCTGTGG CAAACCTGCT GGGCCTCTTC
    TACATCCCCC AGGTCAGCTA TGCCTCCTCC AGCAGACTCC TCAGCAATAA GAATCAGTTC
    AAGTCCTTCC TCCGTACCAT CCCCAATGAT GAACACCAGG CCACTGCCAT GGCAGACATT
    ATTGAGTATT TCCGCTGGAA CTGGGTGGGC ACCATTGCAG CTGATGATGA CTACGGCCGG
    CCAGGGATTG AGAAGTTCCG AGAGGAAGCA GAGGAGAGGG ACATCTGCAT CGACTTCAGT
    GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATTCAGC AAGTGGTAGA GGTGATCCAG
    AATTCCACAG CCAAAGTCAT TGTTGTTTTC TCCAGTGGCC CAGACCTTGA ACCCCTCATC
    AAGGAGATCG TCCGGCGAAA TATCACAGGA AGGATTTGGC TGGCCAGTGA GGCCTGGGCC
    AGCTCTTCCT TGATTGCCAT GCCCGAGTAC TTCCATGTGG TTGGAGGTAC CATTGGATTC
    GCTTTGAAGG CTGGGCAGAT CCCAGGTTTC CGGGAATTCC TGCAGAAAGT CCATCCCAGA
    AAGTCTGTCC ACAACGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
    CAAGAAGGTG CTAAAGGGCC TTTATCCATG GACACTTTCC TGAGAGGCCA CGAAGAAGGT
    GGTGGCAGGA TAAGCAACAG CTCCACTGCC TTCCGACCTC TTTGCACAGG AGATGAGAAC
    ATCAGTAGTG TGGAGACCCC TTATATGGAT TATACACACT TACGGATATC CTACAACGTC
    TACTTAGCAG TCTATTCCAT TGCTCATGCC CTGCAAGATA TATATACATG CTTACCTGGG
    AGAGGGCTCT TCACCAACGG TTCCTGTGCT GATATTAAGA AGGTTGAGGC TTGGCAGGTC
    TTGAAGCACC TACGGCACCT AAACTTTACC AACAATATGG GGGAGCAAGT GACTTTCGAT
    GAATGTGGTG ACCTGATGGG GAACTATTCC ATCATCAACT GGCACCTCTC TCCAGAGGAT
    GGCTCCATAG TGTTTAAGGA AGTCGGATAT TACAATGTCT ATGCCAAGAA AGGAGAAAGA
    CTCTTCATCA ATGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGAT GCCATTTTCC
    AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGAA TCATTGAGGG GGAGCCTACC
    TGCTGCTTTG AGTGTGTGGA GTGCCCCGAC GGGGAGTACA GTGATGAAAC AGATGCAAGT
    GCCTGTGACA AGTGCCCCGA TGACTTCTGG TCCAATGAAA ACCACACTTC GTGCATTGCC
    AAAGAGATTG AGTTTCTGTC CTGGACAGAG CCCTTTGGGA TTGCACTCAC CCTCTTTGCT
    GTGCTGGGCA TTTTCCTGAC AGCTTTCGTG CTGGGGGTCT TCATCAAGTT CCGTAACACG
    CCCATCGTCA AGGCCACCAA CCGAGAGCTC TCGTACCTCC TCCTCTTCTC CTTGCTGTGC
    TGCTTCTCCA GCTCCCTGTT CTTCATTGGC GAGCCCCAGG ACTGGACCTG CCGCCTGCGC
    CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATATCAT GCATCCTGGT GAAAACCAAC
    CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACAAGCT TCCACCGCAA GTGGTGGGGG
    CTCAACCTGC AGTTCCTGCT GGTCTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
    ATCTGGCTCT ACACGGCGCC TCCCTCCAGC TACCGCAACC ATGAGCTGGA GGACGAGATC
    ATCTTCATCA CATGCCACGA GGGCTCCCTG ATGGCCCTGG GCTTCCTGAT TGGCTACACC
    TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
    TTCAACGAGG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
    TTCATTCCAG CCTACGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTGGA AGTGATCGCC
    ATCCTGGCCG CCAGCTTTGG CCTCCTGGCC TGCATCTTCT TCAACAAGGT GTACATCATC
    CTCTTCAAGC CGTCCCGCAA CACCATCGAG GAGGTGCGCT GCAGCACCGC GGCTCACGCT
    TTCAAGGTCG CGGCCCGCGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCGGC
    AGCCTGGGGG GCTCCACGGG CTCCACGCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGT
    GAAGACCCCT TCCCGCAGCC CGAGAGGCAG AAGCAGCAGC AGCCCCTGGC CCTGACCCAG
    CGGGAGCAGC AGCCGCCGCA GCCCTTGACC TTGCCGCCGC AGCCGCAGCC CAGGTGCAAG
    CAGAAGGTCA TCTTCGGCAG TGGCACCGTC ACCTTCTCGC TGAGCTTTGA CGAGCCGCAG
    AAGAGCGCCG CGGCCCCCCG CAATTCCACG CTGCAGCACT CCCTGGAGGC CCAGCGGAGC
    CCCGAGCCCC CCGCCAGACC CCAGGCGTTA CTGCCGCCGC AGGGCGGAGA CACAGACGCG
    GAGCTGCCGG CCCAGGAGCC GGGCCTGCAG GGCCCCGGGG GTGCGGACCG CCGCCCGGAG
    ATGCGAGACC CCGAAGAGCT GTCCCCAGCC CTGGTGGTGT CCAGCTCACA AAGCTTTGTC
    ATCAGCGGCG GAGGCAGCAC GGTCACGGAA AACATACTGC ATTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965618.1
    CDS390..3617
    Translation

    Target ORF information:

    RefSeq Version XM_014110143.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris calcium sensing receptor (CASR), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110143.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATTTC ACAGCTGCTC TTTGATCCTC TTGGCAATCA CCTGGTGCAC TTCTGCCTAT 
    GGGCCTGACC AACGAGCCCA GAAGAAAGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
    CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTAAAGTCAA GGCCGGAGTC TGTGGAATGT
    ATCAGGTACA ATTTCCGCGG GTTTCGTTGG TTACAAGCAA TGATATTTGC CATCGAGGAA
    ATAAACAGCA GCCCAGCCCT TCTTCCAAAC ATGACACTGG GATACAGAAT ATTTGACACT
    TGCAACACCG TTTCTAAAGC CTTGGAGGCC ACTCTGAGTT TTGTGGCACA GAATAAAATT
    GATTCTCTGA ACCTTGACGA GTTCTGCAAC TGCTCAGAGC ATATCCCCTC TACTATCGCT
    GTGGTGGGAG CAACTGGCTC GGGCATCTCC ACGGCTGTGG CAAACCTGCT GGGCCTCTTC
    TACATCCCCC AGGTCAGCTA TGCCTCCTCC AGCAGACTCC TCAGCAATAA GAATCAGTTC
    AAGTCCTTCC TCCGTACCAT CCCCAATGAT GAACACCAGG CCACTGCCAT GGCAGACATT
    ATTGAGTATT TCCGCTGGAA CTGGGTGGGC ACCATTGCAG CTGATGATGA CTACGGCCGG
    CCAGGGATTG AGAAGTTCCG AGAGGAAGCA GAGGAGAGGG ACATCTGCAT CGACTTCAGT
    GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATTCAGC AAGTGGTAGA GGTGATCCAG
    AATTCCACAG CCAAAGTCAT TGTTGTTTTC TCCAGTGGCC CAGACCTTGA ACCCCTCATC
    AAGGAGATCG TCCGGCGAAA TATCACAGGA AGGATTTGGC TGGCCAGTGA GGCCTGGGCC
    AGCTCTTCCT TGATTGCCAT GCCCGAGTAC TTCCATGTGG TTGGAGGTAC CATTGGATTC
    GCTTTGAAGG CTGGGCAGAT CCCAGGTTTC CGGGAATTCC TGCAGAAAGT CCATCCCAGA
    AAGTCTGTCC ACAACGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
    CAAGAAGGTG CTAAAGGGCC TTTATCCATG GACACTTTCC TGAGAGGCCA CGAAGAAGGT
    GGTGGCAGGA TAAGCAACAG CTCCACTGCC TTCCGACCTC TTTGCACAGG AGATGAGAAC
    ATCAGTAGTG TGGAGACCCC TTATATGGAT TATACACACT TACGGATATC CTACAACGTC
    TACTTAGCAG TCTATTCCAT TGCTCATGCC CTGCAAGATA TATATACATG CTTACCTGGG
    AGAGGGCTCT TCACCAACGG TTCCTGTGCT GATATTAAGA AGGTTGAGGC TTGGCAGGTC
    TTGAAGCACC TACGGCACCT AAACTTTACC AACAATATGG GGGAGCAAGT GACTTTCGAT
    GAATGTGGTG ACCTGATGGG GAACTATTCC ATCATCAACT GGCACCTCTC TCCAGAGGAT
    GGCTCCATAG TGTTTAAGGA AGTCGGATAT TACAATGTCT ATGCCAAGAA AGGAGAAAGA
    CTCTTCATCA ATGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGAT GCCATTTTCC
    AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGAA TCATTGAGGG GGAGCCTACC
    TGCTGCTTTG AGTGTGTGGA GTGCCCCGAC GGGGAGTACA GTGATGAAAC AGATGCAAGT
    GCCTGTGACA AGTGCCCCGA TGACTTCTGG TCCAATGAAA ACCACACTTC GTGCATTGCC
    AAAGAGATTG AGTTTCTGTC CTGGACAGAG CCCTTTGGGA TTGCACTCAC CCTCTTTGCT
    GTGCTGGGCA TTTTCCTGAC AGCTTTCGTG CTGGGGGTCT TCATCAAGTT CCGTAACACG
    CCCATCGTCA AGGCCACCAA CCGAGAGCTC TCGTACCTCC TCCTCTTCTC CTTGCTGTGC
    TGCTTCTCCA GCTCCCTGTT CTTCATTGGC GAGCCCCAGG ACTGGACCTG CCGCCTGCGC
    CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATATCAT GCATCCTGGT GAAAACCAAC
    CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACAAGCT TCCACCGCAA GTGGTGGGGG
    CTCAACCTGC AGTTCCTGCT GGTCTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
    ATCTGGCTCT ACACGGCGCC TCCCTCCAGC TACCGCAACC ATGAGCTGGA GGACGAGATC
    ATCTTCATCA CATGCCACGA GGGCTCCCTG ATGGCCCTGG GCTTCCTGAT TGGCTACACC
    TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
    TTCAACGAGG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
    TTCATTCCAG CCTACGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTGGA AGTGATCGCC
    ATCCTGGCCG CCAGCTTTGG CCTCCTGGCC TGCATCTTCT TCAACAAGGT GTACATCATC
    CTCTTCAAGC CGTCCCGCAA CACCATCGAG GAGGTGCGCT GCAGCACCGC GGCTCACGCT
    TTCAAGGTCG CGGCCCGCGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCGGC
    AGCCTGGGGG GCTCCACGGG CTCCACGCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGT
    GAAGACCCCT TCCCGCAGCC CGAGAGGCAG AAGCAGCAGC AGCCCCTGGC CCTGACCCAG
    CGGGAGCAGC AGCCGCCGCA GCCCTTGACC TTGCCGCCGC AGCCGCAGCC CAGGTGCAAG
    CAGAAGGTCA TCTTCGGCAG TGGCACCGTC ACCTTCTCGC TGAGCTTTGA CGAGCCGCAG
    AAGAGCGCCG CGGCCCCCCG CAATTCCACG CTGCAGCACT CCCTGGAGGC CCAGCGGAGC
    CCCGAGCCCC CCGCCAGACC CCAGGCGTTA CTGCCGCCGC AGGGCGGAGA CACAGACGCG
    GAGCTGCCGG CCCAGGAGCC GGGCCTGCAG GGCCCCGGGG GTGCGGACCG CCGCCCGGAG
    ATGCGAGACC CCGAAGAGCT GTCCCCAGCC CTGGTGGTGT CCAGCTCACA AAGCTTTGTC
    ATCAGCGGCG GAGGCAGCAC GGTCACGGAA AACATACTGC ATTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30072
    Clone ID Related Accession (Same CDS sequence) XM_014110142.1 , NM_001081509.2 , XM_014110141.2 , XM_014110143.2 , XM_022413728.1 , XM_014110143.1 , XM_022413727.1 , XM_022413729.1 , XM_014110141.1 , XM_022413726.1 , XM_014110142.2
    Accession Version XM_022413728.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product extracellular calcium-sensing receptor isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006615.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATTTC ACAGCTGCTC TTTGATCCTC TTGGCAATCA CCTGGTGCAC TTCTGCCTAT 
    GGGCCTGACC AACGAGCCCA GAAGAAAGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
    CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTAAAGTCAA GGCCGGAGTC TGTGGAATGT
    ATCAGGTACA ATTTCCGCGG GTTTCGTTGG TTACAAGCAA TGATATTTGC CATCGAGGAA
    ATAAACAGCA GCCCAGCCCT TCTTCCAAAC ATGACACTGG GATACAGAAT ATTTGACACT
    TGCAACACCG TTTCTAAAGC CTTGGAGGCC ACTCTGAGTT TTGTGGCACA GAATAAAATT
    GATTCTCTGA ACCTTGACGA GTTCTGCAAC TGCTCAGAGC ATATCCCCTC TACTATCGCT
    GTGGTGGGAG CAACTGGCTC GGGCATCTCC ACGGCTGTGG CAAACCTGCT GGGCCTCTTC
    TACATCCCCC AGGTCAGCTA TGCCTCCTCC AGCAGACTCC TCAGCAATAA GAATCAGTTC
    AAGTCCTTCC TCCGTACCAT CCCCAATGAT GAACACCAGG CCACTGCCAT GGCAGACATT
    ATTGAGTATT TCCGCTGGAA CTGGGTGGGC ACCATTGCAG CTGATGATGA CTACGGCCGG
    CCAGGGATTG AGAAGTTCCG AGAGGAAGCA GAGGAGAGGG ACATCTGCAT CGACTTCAGT
    GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATTCAGC AAGTGGTAGA GGTGATCCAG
    AATTCCACAG CCAAAGTCAT TGTTGTTTTC TCCAGTGGCC CAGACCTTGA ACCCCTCATC
    AAGGAGATCG TCCGGCGAAA TATCACAGGA AGGATTTGGC TGGCCAGTGA GGCCTGGGCC
    AGCTCTTCCT TGATTGCCAT GCCCGAGTAC TTCCATGTGG TTGGAGGTAC CATTGGATTC
    GCTTTGAAGG CTGGGCAGAT CCCAGGTTTC CGGGAATTCC TGCAGAAAGT CCATCCCAGA
    AAGTCTGTCC ACAACGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
    CAAGAAGGTG CTAAAGGGCC TTTATCCATG GACACTTTCC TGAGAGGCCA CGAAGAAGGT
    GGTGGCAGGA TAAGCAACAG CTCCACTGCC TTCCGACCTC TTTGCACAGG AGATGAGAAC
    ATCAGTAGTG TGGAGACCCC TTATATGGAT TATACACACT TACGGATATC CTACAACGTC
    TACTTAGCAG TCTATTCCAT TGCTCATGCC CTGCAAGATA TATATACATG CTTACCTGGG
    AGAGGGCTCT TCACCAACGG TTCCTGTGCT GATATTAAGA AGGTTGAGGC TTGGCAGGTC
    TTGAAGCACC TACGGCACCT AAACTTTACC AACAATATGG GGGAGCAAGT GACTTTCGAT
    GAATGTGGTG ACCTGATGGG GAACTATTCC ATCATCAACT GGCACCTCTC TCCAGAGGAT
    GGCTCCATAG TGTTTAAGGA AGTCGGATAT TACAATGTCT ATGCCAAGAA AGGAGAAAGA
    CTCTTCATCA ATGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGAT GCCATTTTCC
    AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGAA TCATTGAGGG GGAGCCTACC
    TGCTGCTTTG AGTGTGTGGA GTGCCCCGAC GGGGAGTACA GTGATGAAAC AGATGCAAGT
    GCCTGTGACA AGTGCCCCGA TGACTTCTGG TCCAATGAAA ACCACACTTC GTGCATTGCC
    AAAGAGATTG AGTTTCTGTC CTGGACAGAG CCCTTTGGGA TTGCACTCAC CCTCTTTGCT
    GTGCTGGGCA TTTTCCTGAC AGCTTTCGTG CTGGGGGTCT TCATCAAGTT CCGTAACACG
    CCCATCGTCA AGGCCACCAA CCGAGAGCTC TCGTACCTCC TCCTCTTCTC CTTGCTGTGC
    TGCTTCTCCA GCTCCCTGTT CTTCATTGGC GAGCCCCAGG ACTGGACCTG CCGCCTGCGC
    CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATATCAT GCATCCTGGT GAAAACCAAC
    CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACAAGCT TCCACCGCAA GTGGTGGGGG
    CTCAACCTGC AGTTCCTGCT GGTCTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
    ATCTGGCTCT ACACGGCGCC TCCCTCCAGC TACCGCAACC ATGAGCTGGA GGACGAGATC
    ATCTTCATCA CATGCCACGA GGGCTCCCTG ATGGCCCTGG GCTTCCTGAT TGGCTACACC
    TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
    TTCAACGAGG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
    TTCATTCCAG CCTACGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTGGA AGTGATCGCC
    ATCCTGGCCG CCAGCTTTGG CCTCCTGGCC TGCATCTTCT TCAACAAGGT GTACATCATC
    CTCTTCAAGC CGTCCCGCAA CACCATCGAG GAGGTGCGCT GCAGCACCGC GGCTCACGCT
    TTCAAGGTCG CGGCCCGCGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCGGC
    AGCCTGGGGG GCTCCACGGG CTCCACGCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGT
    GAAGACCCCT TCCCGCAGCC CGAGAGGCAG AAGCAGCAGC AGCCCCTGGC CCTGACCCAG
    CGGGAGCAGC AGCCGCCGCA GCCCTTGACC TTGCCGCCGC AGCCGCAGCC CAGGTGCAAG
    CAGAAGGTCA TCTTCGGCAG TGGCACCGTC ACCTTCTCGC TGAGCTTTGA CGAGCCGCAG
    AAGAGCGCCG CGGCCCCCCG CAATTCCACG CTGCAGCACT CCCTGGAGGC CCAGCGGAGC
    CCCGAGCCCC CCGCCAGACC CCAGGCGTTA CTGCCGCCGC AGGGCGGAGA CACAGACGCG
    GAGCTGCCGG CCCAGGAGCC GGGCCTGCAG GGCCCCGGGG GTGCGGACCG CCGCCCGGAG
    ATGCGAGACC CCGAAGAGCT GTCCCCAGCC CTGGTGGTGT CCAGCTCACA AAGCTTTGTC
    ATCAGCGGCG GAGGCAGCAC GGTCACGGAA AACATACTGC ATTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269436.1
    CDS705..3932
    Translation

    Target ORF information:

    RefSeq Version XM_022413728.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris calcium sensing receptor (CASR), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413728.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATTTC ACAGCTGCTC TTTGATCCTC TTGGCAATCA CCTGGTGCAC TTCTGCCTAT 
    GGGCCTGACC AACGAGCCCA GAAGAAAGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
    CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTAAAGTCAA GGCCGGAGTC TGTGGAATGT
    ATCAGGTACA ATTTCCGCGG GTTTCGTTGG TTACAAGCAA TGATATTTGC CATCGAGGAA
    ATAAACAGCA GCCCAGCCCT TCTTCCAAAC ATGACACTGG GATACAGAAT ATTTGACACT
    TGCAACACCG TTTCTAAAGC CTTGGAGGCC ACTCTGAGTT TTGTGGCACA GAATAAAATT
    GATTCTCTGA ACCTTGACGA GTTCTGCAAC TGCTCAGAGC ATATCCCCTC TACTATCGCT
    GTGGTGGGAG CAACTGGCTC GGGCATCTCC ACGGCTGTGG CAAACCTGCT GGGCCTCTTC
    TACATCCCCC AGGTCAGCTA TGCCTCCTCC AGCAGACTCC TCAGCAATAA GAATCAGTTC
    AAGTCCTTCC TCCGTACCAT CCCCAATGAT GAACACCAGG CCACTGCCAT GGCAGACATT
    ATTGAGTATT TCCGCTGGAA CTGGGTGGGC ACCATTGCAG CTGATGATGA CTACGGCCGG
    CCAGGGATTG AGAAGTTCCG AGAGGAAGCA GAGGAGAGGG ACATCTGCAT CGACTTCAGT
    GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATTCAGC AAGTGGTAGA GGTGATCCAG
    AATTCCACAG CCAAAGTCAT TGTTGTTTTC TCCAGTGGCC CAGACCTTGA ACCCCTCATC
    AAGGAGATCG TCCGGCGAAA TATCACAGGA AGGATTTGGC TGGCCAGTGA GGCCTGGGCC
    AGCTCTTCCT TGATTGCCAT GCCCGAGTAC TTCCATGTGG TTGGAGGTAC CATTGGATTC
    GCTTTGAAGG CTGGGCAGAT CCCAGGTTTC CGGGAATTCC TGCAGAAAGT CCATCCCAGA
    AAGTCTGTCC ACAACGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
    CAAGAAGGTG CTAAAGGGCC TTTATCCATG GACACTTTCC TGAGAGGCCA CGAAGAAGGT
    GGTGGCAGGA TAAGCAACAG CTCCACTGCC TTCCGACCTC TTTGCACAGG AGATGAGAAC
    ATCAGTAGTG TGGAGACCCC TTATATGGAT TATACACACT TACGGATATC CTACAACGTC
    TACTTAGCAG TCTATTCCAT TGCTCATGCC CTGCAAGATA TATATACATG CTTACCTGGG
    AGAGGGCTCT TCACCAACGG TTCCTGTGCT GATATTAAGA AGGTTGAGGC TTGGCAGGTC
    TTGAAGCACC TACGGCACCT AAACTTTACC AACAATATGG GGGAGCAAGT GACTTTCGAT
    GAATGTGGTG ACCTGATGGG GAACTATTCC ATCATCAACT GGCACCTCTC TCCAGAGGAT
    GGCTCCATAG TGTTTAAGGA AGTCGGATAT TACAATGTCT ATGCCAAGAA AGGAGAAAGA
    CTCTTCATCA ATGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGAT GCCATTTTCC
    AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGAA TCATTGAGGG GGAGCCTACC
    TGCTGCTTTG AGTGTGTGGA GTGCCCCGAC GGGGAGTACA GTGATGAAAC AGATGCAAGT
    GCCTGTGACA AGTGCCCCGA TGACTTCTGG TCCAATGAAA ACCACACTTC GTGCATTGCC
    AAAGAGATTG AGTTTCTGTC CTGGACAGAG CCCTTTGGGA TTGCACTCAC CCTCTTTGCT
    GTGCTGGGCA TTTTCCTGAC AGCTTTCGTG CTGGGGGTCT TCATCAAGTT CCGTAACACG
    CCCATCGTCA AGGCCACCAA CCGAGAGCTC TCGTACCTCC TCCTCTTCTC CTTGCTGTGC
    TGCTTCTCCA GCTCCCTGTT CTTCATTGGC GAGCCCCAGG ACTGGACCTG CCGCCTGCGC
    CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATATCAT GCATCCTGGT GAAAACCAAC
    CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACAAGCT TCCACCGCAA GTGGTGGGGG
    CTCAACCTGC AGTTCCTGCT GGTCTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
    ATCTGGCTCT ACACGGCGCC TCCCTCCAGC TACCGCAACC ATGAGCTGGA GGACGAGATC
    ATCTTCATCA CATGCCACGA GGGCTCCCTG ATGGCCCTGG GCTTCCTGAT TGGCTACACC
    TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
    TTCAACGAGG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
    TTCATTCCAG CCTACGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTGGA AGTGATCGCC
    ATCCTGGCCG CCAGCTTTGG CCTCCTGGCC TGCATCTTCT TCAACAAGGT GTACATCATC
    CTCTTCAAGC CGTCCCGCAA CACCATCGAG GAGGTGCGCT GCAGCACCGC GGCTCACGCT
    TTCAAGGTCG CGGCCCGCGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCGGC
    AGCCTGGGGG GCTCCACGGG CTCCACGCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGT
    GAAGACCCCT TCCCGCAGCC CGAGAGGCAG AAGCAGCAGC AGCCCCTGGC CCTGACCCAG
    CGGGAGCAGC AGCCGCCGCA GCCCTTGACC TTGCCGCCGC AGCCGCAGCC CAGGTGCAAG
    CAGAAGGTCA TCTTCGGCAG TGGCACCGTC ACCTTCTCGC TGAGCTTTGA CGAGCCGCAG
    AAGAGCGCCG CGGCCCCCCG CAATTCCACG CTGCAGCACT CCCTGGAGGC CCAGCGGAGC
    CCCGAGCCCC CCGCCAGACC CCAGGCGTTA CTGCCGCCGC AGGGCGGAGA CACAGACGCG
    GAGCTGCCGG CCCAGGAGCC GGGCCTGCAG GGCCCCGGGG GTGCGGACCG CCGCCCGGAG
    ATGCGAGACC CCGAAGAGCT GTCCCCAGCC CTGGTGGTGT CCAGCTCACA AAGCTTTGTC
    ATCAGCGGCG GAGGCAGCAC GGTCACGGAA AACATACTGC ATTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30072
    Clone ID Related Accession (Same CDS sequence) XM_014110142.1 , NM_001081509.2 , XM_014110141.2 , XM_014110143.2 , XM_022413728.1 , XM_014110143.1 , XM_022413727.1 , XM_022413729.1 , XM_014110141.1 , XM_022413726.1 , XM_014110142.2
    Accession Version XM_014110143.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product extracellular calcium-sensing receptor isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726118.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATTTC ACAGCTGCTC TTTGATCCTC TTGGCAATCA CCTGGTGCAC TTCTGCCTAT 
    GGGCCTGACC AACGAGCCCA GAAGAAAGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
    CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTAAAGTCAA GGCCGGAGTC TGTGGAATGT
    ATCAGGTACA ATTTCCGCGG GTTTCGTTGG TTACAAGCAA TGATATTTGC CATCGAGGAA
    ATAAACAGCA GCCCAGCCCT TCTTCCAAAC ATGACACTGG GATACAGAAT ATTTGACACT
    TGCAACACCG TTTCTAAAGC CTTGGAGGCC ACTCTGAGTT TTGTGGCACA GAATAAAATT
    GATTCTCTGA ACCTTGACGA GTTCTGCAAC TGCTCAGAGC ATATCCCCTC TACTATCGCT
    GTGGTGGGAG CAACTGGCTC GGGCATCTCC ACGGCTGTGG CAAACCTGCT GGGCCTCTTC
    TACATCCCCC AGGTCAGCTA TGCCTCCTCC AGCAGACTCC TCAGCAATAA GAATCAGTTC
    AAGTCCTTCC TCCGTACCAT CCCCAATGAT GAACACCAGG CCACTGCCAT GGCAGACATT
    ATTGAGTATT TCCGCTGGAA CTGGGTGGGC ACCATTGCAG CTGATGATGA CTACGGCCGG
    CCAGGGATTG AGAAGTTCCG AGAGGAAGCA GAGGAGAGGG ACATCTGCAT CGACTTCAGT
    GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATTCAGC AAGTGGTAGA GGTGATCCAG
    AATTCCACAG CCAAAGTCAT TGTTGTTTTC TCCAGTGGCC CAGACCTTGA ACCCCTCATC
    AAGGAGATCG TCCGGCGAAA TATCACAGGA AGGATTTGGC TGGCCAGTGA GGCCTGGGCC
    AGCTCTTCCT TGATTGCCAT GCCCGAGTAC TTCCATGTGG TTGGAGGTAC CATTGGATTC
    GCTTTGAAGG CTGGGCAGAT CCCAGGTTTC CGGGAATTCC TGCAGAAAGT CCATCCCAGA
    AAGTCTGTCC ACAACGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
    CAAGAAGGTG CTAAAGGGCC TTTATCCATG GACACTTTCC TGAGAGGCCA CGAAGAAGGT
    GGTGGCAGGA TAAGCAACAG CTCCACTGCC TTCCGACCTC TTTGCACAGG AGATGAGAAC
    ATCAGTAGTG TGGAGACCCC TTATATGGAT TATACACACT TACGGATATC CTACAACGTC
    TACTTAGCAG TCTATTCCAT TGCTCATGCC CTGCAAGATA TATATACATG CTTACCTGGG
    AGAGGGCTCT TCACCAACGG TTCCTGTGCT GATATTAAGA AGGTTGAGGC TTGGCAGGTC
    TTGAAGCACC TACGGCACCT AAACTTTACC AACAATATGG GGGAGCAAGT GACTTTCGAT
    GAATGTGGTG ACCTGATGGG GAACTATTCC ATCATCAACT GGCACCTCTC TCCAGAGGAT
    GGCTCCATAG TGTTTAAGGA AGTCGGATAT TACAATGTCT ATGCCAAGAA AGGAGAAAGA
    CTCTTCATCA ATGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGAT GCCATTTTCC
    AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGAA TCATTGAGGG GGAGCCTACC
    TGCTGCTTTG AGTGTGTGGA GTGCCCCGAC GGGGAGTACA GTGATGAAAC AGATGCAAGT
    GCCTGTGACA AGTGCCCCGA TGACTTCTGG TCCAATGAAA ACCACACTTC GTGCATTGCC
    AAAGAGATTG AGTTTCTGTC CTGGACAGAG CCCTTTGGGA TTGCACTCAC CCTCTTTGCT
    GTGCTGGGCA TTTTCCTGAC AGCTTTCGTG CTGGGGGTCT TCATCAAGTT CCGTAACACG
    CCCATCGTCA AGGCCACCAA CCGAGAGCTC TCGTACCTCC TCCTCTTCTC CTTGCTGTGC
    TGCTTCTCCA GCTCCCTGTT CTTCATTGGC GAGCCCCAGG ACTGGACCTG CCGCCTGCGC
    CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATATCAT GCATCCTGGT GAAAACCAAC
    CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACAAGCT TCCACCGCAA GTGGTGGGGG
    CTCAACCTGC AGTTCCTGCT GGTCTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
    ATCTGGCTCT ACACGGCGCC TCCCTCCAGC TACCGCAACC ATGAGCTGGA GGACGAGATC
    ATCTTCATCA CATGCCACGA GGGCTCCCTG ATGGCCCTGG GCTTCCTGAT TGGCTACACC
    TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
    TTCAACGAGG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
    TTCATTCCAG CCTACGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTGGA AGTGATCGCC
    ATCCTGGCCG CCAGCTTTGG CCTCCTGGCC TGCATCTTCT TCAACAAGGT GTACATCATC
    CTCTTCAAGC CGTCCCGCAA CACCATCGAG GAGGTGCGCT GCAGCACCGC GGCTCACGCT
    TTCAAGGTCG CGGCCCGCGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCGGC
    AGCCTGGGGG GCTCCACGGG CTCCACGCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGT
    GAAGACCCCT TCCCGCAGCC CGAGAGGCAG AAGCAGCAGC AGCCCCTGGC CCTGACCCAG
    CGGGAGCAGC AGCCGCCGCA GCCCTTGACC TTGCCGCCGC AGCCGCAGCC CAGGTGCAAG
    CAGAAGGTCA TCTTCGGCAG TGGCACCGTC ACCTTCTCGC TGAGCTTTGA CGAGCCGCAG
    AAGAGCGCCG CGGCCCCCCG CAATTCCACG CTGCAGCACT CCCTGGAGGC CCAGCGGAGC
    CCCGAGCCCC CCGCCAGACC CCAGGCGTTA CTGCCGCCGC AGGGCGGAGA CACAGACGCG
    GAGCTGCCGG CCCAGGAGCC GGGCCTGCAG GGCCCCGGGG GTGCGGACCG CCGCCCGGAG
    ATGCGAGACC CCGAAGAGCT GTCCCCAGCC CTGGTGGTGT CCAGCTCACA AAGCTTTGTC
    ATCAGCGGCG GAGGCAGCAC GGTCACGGAA AACATACTGC ATTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965618.1
    CDS352..3579
    Translation

    Target ORF information:

    RefSeq Version XM_014110143.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris calcium-sensing receptor (CASR), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110143.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATTTC ACAGCTGCTC TTTGATCCTC TTGGCAATCA CCTGGTGCAC TTCTGCCTAT 
    GGGCCTGACC AACGAGCCCA GAAGAAAGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
    CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTAAAGTCAA GGCCGGAGTC TGTGGAATGT
    ATCAGGTACA ATTTCCGCGG GTTTCGTTGG TTACAAGCAA TGATATTTGC CATCGAGGAA
    ATAAACAGCA GCCCAGCCCT TCTTCCAAAC ATGACACTGG GATACAGAAT ATTTGACACT
    TGCAACACCG TTTCTAAAGC CTTGGAGGCC ACTCTGAGTT TTGTGGCACA GAATAAAATT
    GATTCTCTGA ACCTTGACGA GTTCTGCAAC TGCTCAGAGC ATATCCCCTC TACTATCGCT
    GTGGTGGGAG CAACTGGCTC GGGCATCTCC ACGGCTGTGG CAAACCTGCT GGGCCTCTTC
    TACATCCCCC AGGTCAGCTA TGCCTCCTCC AGCAGACTCC TCAGCAATAA GAATCAGTTC
    AAGTCCTTCC TCCGTACCAT CCCCAATGAT GAACACCAGG CCACTGCCAT GGCAGACATT
    ATTGAGTATT TCCGCTGGAA CTGGGTGGGC ACCATTGCAG CTGATGATGA CTACGGCCGG
    CCAGGGATTG AGAAGTTCCG AGAGGAAGCA GAGGAGAGGG ACATCTGCAT CGACTTCAGT
    GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATTCAGC AAGTGGTAGA GGTGATCCAG
    AATTCCACAG CCAAAGTCAT TGTTGTTTTC TCCAGTGGCC CAGACCTTGA ACCCCTCATC
    AAGGAGATCG TCCGGCGAAA TATCACAGGA AGGATTTGGC TGGCCAGTGA GGCCTGGGCC
    AGCTCTTCCT TGATTGCCAT GCCCGAGTAC TTCCATGTGG TTGGAGGTAC CATTGGATTC
    GCTTTGAAGG CTGGGCAGAT CCCAGGTTTC CGGGAATTCC TGCAGAAAGT CCATCCCAGA
    AAGTCTGTCC ACAACGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
    CAAGAAGGTG CTAAAGGGCC TTTATCCATG GACACTTTCC TGAGAGGCCA CGAAGAAGGT
    GGTGGCAGGA TAAGCAACAG CTCCACTGCC TTCCGACCTC TTTGCACAGG AGATGAGAAC
    ATCAGTAGTG TGGAGACCCC TTATATGGAT TATACACACT TACGGATATC CTACAACGTC
    TACTTAGCAG TCTATTCCAT TGCTCATGCC CTGCAAGATA TATATACATG CTTACCTGGG
    AGAGGGCTCT TCACCAACGG TTCCTGTGCT GATATTAAGA AGGTTGAGGC TTGGCAGGTC
    TTGAAGCACC TACGGCACCT AAACTTTACC AACAATATGG GGGAGCAAGT GACTTTCGAT
    GAATGTGGTG ACCTGATGGG GAACTATTCC ATCATCAACT GGCACCTCTC TCCAGAGGAT
    GGCTCCATAG TGTTTAAGGA AGTCGGATAT TACAATGTCT ATGCCAAGAA AGGAGAAAGA
    CTCTTCATCA ATGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGAT GCCATTTTCC
    AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGAA TCATTGAGGG GGAGCCTACC
    TGCTGCTTTG AGTGTGTGGA GTGCCCCGAC GGGGAGTACA GTGATGAAAC AGATGCAAGT
    GCCTGTGACA AGTGCCCCGA TGACTTCTGG TCCAATGAAA ACCACACTTC GTGCATTGCC
    AAAGAGATTG AGTTTCTGTC CTGGACAGAG CCCTTTGGGA TTGCACTCAC CCTCTTTGCT
    GTGCTGGGCA TTTTCCTGAC AGCTTTCGTG CTGGGGGTCT TCATCAAGTT CCGTAACACG
    CCCATCGTCA AGGCCACCAA CCGAGAGCTC TCGTACCTCC TCCTCTTCTC CTTGCTGTGC
    TGCTTCTCCA GCTCCCTGTT CTTCATTGGC GAGCCCCAGG ACTGGACCTG CCGCCTGCGC
    CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATATCAT GCATCCTGGT GAAAACCAAC
    CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACAAGCT TCCACCGCAA GTGGTGGGGG
    CTCAACCTGC AGTTCCTGCT GGTCTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
    ATCTGGCTCT ACACGGCGCC TCCCTCCAGC TACCGCAACC ATGAGCTGGA GGACGAGATC
    ATCTTCATCA CATGCCACGA GGGCTCCCTG ATGGCCCTGG GCTTCCTGAT TGGCTACACC
    TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
    TTCAACGAGG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
    TTCATTCCAG CCTACGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTGGA AGTGATCGCC
    ATCCTGGCCG CCAGCTTTGG CCTCCTGGCC TGCATCTTCT TCAACAAGGT GTACATCATC
    CTCTTCAAGC CGTCCCGCAA CACCATCGAG GAGGTGCGCT GCAGCACCGC GGCTCACGCT
    TTCAAGGTCG CGGCCCGCGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCGGC
    AGCCTGGGGG GCTCCACGGG CTCCACGCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGT
    GAAGACCCCT TCCCGCAGCC CGAGAGGCAG AAGCAGCAGC AGCCCCTGGC CCTGACCCAG
    CGGGAGCAGC AGCCGCCGCA GCCCTTGACC TTGCCGCCGC AGCCGCAGCC CAGGTGCAAG
    CAGAAGGTCA TCTTCGGCAG TGGCACCGTC ACCTTCTCGC TGAGCTTTGA CGAGCCGCAG
    AAGAGCGCCG CGGCCCCCCG CAATTCCACG CTGCAGCACT CCCTGGAGGC CCAGCGGAGC
    CCCGAGCCCC CCGCCAGACC CCAGGCGTTA CTGCCGCCGC AGGGCGGAGA CACAGACGCG
    GAGCTGCCGG CCCAGGAGCC GGGCCTGCAG GGCCCCGGGG GTGCGGACCG CCGCCCGGAG
    ATGCGAGACC CCGAAGAGCT GTCCCCAGCC CTGGTGGTGT CCAGCTCACA AAGCTTTGTC
    ATCAGCGGCG GAGGCAGCAC GGTCACGGAA AACATACTGC ATTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30072
    Clone ID Related Accession (Same CDS sequence) XM_014110142.1 , NM_001081509.2 , XM_014110141.2 , XM_014110143.2 , XM_022413728.1 , XM_014110143.1 , XM_022413727.1 , XM_022413729.1 , XM_014110141.1 , XM_022413726.1 , XM_014110142.2
    Accession Version XM_022413727.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product extracellular calcium-sensing receptor isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006615.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATTTC ACAGCTGCTC TTTGATCCTC TTGGCAATCA CCTGGTGCAC TTCTGCCTAT 
    GGGCCTGACC AACGAGCCCA GAAGAAAGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
    CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTAAAGTCAA GGCCGGAGTC TGTGGAATGT
    ATCAGGTACA ATTTCCGCGG GTTTCGTTGG TTACAAGCAA TGATATTTGC CATCGAGGAA
    ATAAACAGCA GCCCAGCCCT TCTTCCAAAC ATGACACTGG GATACAGAAT ATTTGACACT
    TGCAACACCG TTTCTAAAGC CTTGGAGGCC ACTCTGAGTT TTGTGGCACA GAATAAAATT
    GATTCTCTGA ACCTTGACGA GTTCTGCAAC TGCTCAGAGC ATATCCCCTC TACTATCGCT
    GTGGTGGGAG CAACTGGCTC GGGCATCTCC ACGGCTGTGG CAAACCTGCT GGGCCTCTTC
    TACATCCCCC AGGTCAGCTA TGCCTCCTCC AGCAGACTCC TCAGCAATAA GAATCAGTTC
    AAGTCCTTCC TCCGTACCAT CCCCAATGAT GAACACCAGG CCACTGCCAT GGCAGACATT
    ATTGAGTATT TCCGCTGGAA CTGGGTGGGC ACCATTGCAG CTGATGATGA CTACGGCCGG
    CCAGGGATTG AGAAGTTCCG AGAGGAAGCA GAGGAGAGGG ACATCTGCAT CGACTTCAGT
    GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATTCAGC AAGTGGTAGA GGTGATCCAG
    AATTCCACAG CCAAAGTCAT TGTTGTTTTC TCCAGTGGCC CAGACCTTGA ACCCCTCATC
    AAGGAGATCG TCCGGCGAAA TATCACAGGA AGGATTTGGC TGGCCAGTGA GGCCTGGGCC
    AGCTCTTCCT TGATTGCCAT GCCCGAGTAC TTCCATGTGG TTGGAGGTAC CATTGGATTC
    GCTTTGAAGG CTGGGCAGAT CCCAGGTTTC CGGGAATTCC TGCAGAAAGT CCATCCCAGA
    AAGTCTGTCC ACAACGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
    CAAGAAGGTG CTAAAGGGCC TTTATCCATG GACACTTTCC TGAGAGGCCA CGAAGAAGGT
    GGTGGCAGGA TAAGCAACAG CTCCACTGCC TTCCGACCTC TTTGCACAGG AGATGAGAAC
    ATCAGTAGTG TGGAGACCCC TTATATGGAT TATACACACT TACGGATATC CTACAACGTC
    TACTTAGCAG TCTATTCCAT TGCTCATGCC CTGCAAGATA TATATACATG CTTACCTGGG
    AGAGGGCTCT TCACCAACGG TTCCTGTGCT GATATTAAGA AGGTTGAGGC TTGGCAGGTC
    TTGAAGCACC TACGGCACCT AAACTTTACC AACAATATGG GGGAGCAAGT GACTTTCGAT
    GAATGTGGTG ACCTGATGGG GAACTATTCC ATCATCAACT GGCACCTCTC TCCAGAGGAT
    GGCTCCATAG TGTTTAAGGA AGTCGGATAT TACAATGTCT ATGCCAAGAA AGGAGAAAGA
    CTCTTCATCA ATGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGAT GCCATTTTCC
    AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGAA TCATTGAGGG GGAGCCTACC
    TGCTGCTTTG AGTGTGTGGA GTGCCCCGAC GGGGAGTACA GTGATGAAAC AGATGCAAGT
    GCCTGTGACA AGTGCCCCGA TGACTTCTGG TCCAATGAAA ACCACACTTC GTGCATTGCC
    AAAGAGATTG AGTTTCTGTC CTGGACAGAG CCCTTTGGGA TTGCACTCAC CCTCTTTGCT
    GTGCTGGGCA TTTTCCTGAC AGCTTTCGTG CTGGGGGTCT TCATCAAGTT CCGTAACACG
    CCCATCGTCA AGGCCACCAA CCGAGAGCTC TCGTACCTCC TCCTCTTCTC CTTGCTGTGC
    TGCTTCTCCA GCTCCCTGTT CTTCATTGGC GAGCCCCAGG ACTGGACCTG CCGCCTGCGC
    CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATATCAT GCATCCTGGT GAAAACCAAC
    CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACAAGCT TCCACCGCAA GTGGTGGGGG
    CTCAACCTGC AGTTCCTGCT GGTCTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
    ATCTGGCTCT ACACGGCGCC TCCCTCCAGC TACCGCAACC ATGAGCTGGA GGACGAGATC
    ATCTTCATCA CATGCCACGA GGGCTCCCTG ATGGCCCTGG GCTTCCTGAT TGGCTACACC
    TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
    TTCAACGAGG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
    TTCATTCCAG CCTACGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTGGA AGTGATCGCC
    ATCCTGGCCG CCAGCTTTGG CCTCCTGGCC TGCATCTTCT TCAACAAGGT GTACATCATC
    CTCTTCAAGC CGTCCCGCAA CACCATCGAG GAGGTGCGCT GCAGCACCGC GGCTCACGCT
    TTCAAGGTCG CGGCCCGCGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCGGC
    AGCCTGGGGG GCTCCACGGG CTCCACGCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGT
    GAAGACCCCT TCCCGCAGCC CGAGAGGCAG AAGCAGCAGC AGCCCCTGGC CCTGACCCAG
    CGGGAGCAGC AGCCGCCGCA GCCCTTGACC TTGCCGCCGC AGCCGCAGCC CAGGTGCAAG
    CAGAAGGTCA TCTTCGGCAG TGGCACCGTC ACCTTCTCGC TGAGCTTTGA CGAGCCGCAG
    AAGAGCGCCG CGGCCCCCCG CAATTCCACG CTGCAGCACT CCCTGGAGGC CCAGCGGAGC
    CCCGAGCCCC CCGCCAGACC CCAGGCGTTA CTGCCGCCGC AGGGCGGAGA CACAGACGCG
    GAGCTGCCGG CCCAGGAGCC GGGCCTGCAG GGCCCCGGGG GTGCGGACCG CCGCCCGGAG
    ATGCGAGACC CCGAAGAGCT GTCCCCAGCC CTGGTGGTGT CCAGCTCACA AAGCTTTGTC
    ATCAGCGGCG GAGGCAGCAC GGTCACGGAA AACATACTGC ATTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269435.1
    CDS878..4105
    Translation

    Target ORF information:

    RefSeq Version XM_022413727.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris calcium sensing receptor (CASR), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413727.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATTTC ACAGCTGCTC TTTGATCCTC TTGGCAATCA CCTGGTGCAC TTCTGCCTAT 
    GGGCCTGACC AACGAGCCCA GAAGAAAGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
    CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTAAAGTCAA GGCCGGAGTC TGTGGAATGT
    ATCAGGTACA ATTTCCGCGG GTTTCGTTGG TTACAAGCAA TGATATTTGC CATCGAGGAA
    ATAAACAGCA GCCCAGCCCT TCTTCCAAAC ATGACACTGG GATACAGAAT ATTTGACACT
    TGCAACACCG TTTCTAAAGC CTTGGAGGCC ACTCTGAGTT TTGTGGCACA GAATAAAATT
    GATTCTCTGA ACCTTGACGA GTTCTGCAAC TGCTCAGAGC ATATCCCCTC TACTATCGCT
    GTGGTGGGAG CAACTGGCTC GGGCATCTCC ACGGCTGTGG CAAACCTGCT GGGCCTCTTC
    TACATCCCCC AGGTCAGCTA TGCCTCCTCC AGCAGACTCC TCAGCAATAA GAATCAGTTC
    AAGTCCTTCC TCCGTACCAT CCCCAATGAT GAACACCAGG CCACTGCCAT GGCAGACATT
    ATTGAGTATT TCCGCTGGAA CTGGGTGGGC ACCATTGCAG CTGATGATGA CTACGGCCGG
    CCAGGGATTG AGAAGTTCCG AGAGGAAGCA GAGGAGAGGG ACATCTGCAT CGACTTCAGT
    GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATTCAGC AAGTGGTAGA GGTGATCCAG
    AATTCCACAG CCAAAGTCAT TGTTGTTTTC TCCAGTGGCC CAGACCTTGA ACCCCTCATC
    AAGGAGATCG TCCGGCGAAA TATCACAGGA AGGATTTGGC TGGCCAGTGA GGCCTGGGCC
    AGCTCTTCCT TGATTGCCAT GCCCGAGTAC TTCCATGTGG TTGGAGGTAC CATTGGATTC
    GCTTTGAAGG CTGGGCAGAT CCCAGGTTTC CGGGAATTCC TGCAGAAAGT CCATCCCAGA
    AAGTCTGTCC ACAACGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
    CAAGAAGGTG CTAAAGGGCC TTTATCCATG GACACTTTCC TGAGAGGCCA CGAAGAAGGT
    GGTGGCAGGA TAAGCAACAG CTCCACTGCC TTCCGACCTC TTTGCACAGG AGATGAGAAC
    ATCAGTAGTG TGGAGACCCC TTATATGGAT TATACACACT TACGGATATC CTACAACGTC
    TACTTAGCAG TCTATTCCAT TGCTCATGCC CTGCAAGATA TATATACATG CTTACCTGGG
    AGAGGGCTCT TCACCAACGG TTCCTGTGCT GATATTAAGA AGGTTGAGGC TTGGCAGGTC
    TTGAAGCACC TACGGCACCT AAACTTTACC AACAATATGG GGGAGCAAGT GACTTTCGAT
    GAATGTGGTG ACCTGATGGG GAACTATTCC ATCATCAACT GGCACCTCTC TCCAGAGGAT
    GGCTCCATAG TGTTTAAGGA AGTCGGATAT TACAATGTCT ATGCCAAGAA AGGAGAAAGA
    CTCTTCATCA ATGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGAT GCCATTTTCC
    AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGAA TCATTGAGGG GGAGCCTACC
    TGCTGCTTTG AGTGTGTGGA GTGCCCCGAC GGGGAGTACA GTGATGAAAC AGATGCAAGT
    GCCTGTGACA AGTGCCCCGA TGACTTCTGG TCCAATGAAA ACCACACTTC GTGCATTGCC
    AAAGAGATTG AGTTTCTGTC CTGGACAGAG CCCTTTGGGA TTGCACTCAC CCTCTTTGCT
    GTGCTGGGCA TTTTCCTGAC AGCTTTCGTG CTGGGGGTCT TCATCAAGTT CCGTAACACG
    CCCATCGTCA AGGCCACCAA CCGAGAGCTC TCGTACCTCC TCCTCTTCTC CTTGCTGTGC
    TGCTTCTCCA GCTCCCTGTT CTTCATTGGC GAGCCCCAGG ACTGGACCTG CCGCCTGCGC
    CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATATCAT GCATCCTGGT GAAAACCAAC
    CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACAAGCT TCCACCGCAA GTGGTGGGGG
    CTCAACCTGC AGTTCCTGCT GGTCTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
    ATCTGGCTCT ACACGGCGCC TCCCTCCAGC TACCGCAACC ATGAGCTGGA GGACGAGATC
    ATCTTCATCA CATGCCACGA GGGCTCCCTG ATGGCCCTGG GCTTCCTGAT TGGCTACACC
    TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
    TTCAACGAGG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
    TTCATTCCAG CCTACGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTGGA AGTGATCGCC
    ATCCTGGCCG CCAGCTTTGG CCTCCTGGCC TGCATCTTCT TCAACAAGGT GTACATCATC
    CTCTTCAAGC CGTCCCGCAA CACCATCGAG GAGGTGCGCT GCAGCACCGC GGCTCACGCT
    TTCAAGGTCG CGGCCCGCGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCGGC
    AGCCTGGGGG GCTCCACGGG CTCCACGCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGT
    GAAGACCCCT TCCCGCAGCC CGAGAGGCAG AAGCAGCAGC AGCCCCTGGC CCTGACCCAG
    CGGGAGCAGC AGCCGCCGCA GCCCTTGACC TTGCCGCCGC AGCCGCAGCC CAGGTGCAAG
    CAGAAGGTCA TCTTCGGCAG TGGCACCGTC ACCTTCTCGC TGAGCTTTGA CGAGCCGCAG
    AAGAGCGCCG CGGCCCCCCG CAATTCCACG CTGCAGCACT CCCTGGAGGC CCAGCGGAGC
    CCCGAGCCCC CCGCCAGACC CCAGGCGTTA CTGCCGCCGC AGGGCGGAGA CACAGACGCG
    GAGCTGCCGG CCCAGGAGCC GGGCCTGCAG GGCCCCGGGG GTGCGGACCG CCGCCCGGAG
    ATGCGAGACC CCGAAGAGCT GTCCCCAGCC CTGGTGGTGT CCAGCTCACA AAGCTTTGTC
    ATCAGCGGCG GAGGCAGCAC GGTCACGGAA AACATACTGC ATTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30072
    Clone ID Related Accession (Same CDS sequence) XM_014110142.1 , NM_001081509.2 , XM_014110141.2 , XM_014110143.2 , XM_022413728.1 , XM_014110143.1 , XM_022413727.1 , XM_022413729.1 , XM_014110141.1 , XM_022413726.1 , XM_014110142.2
    Accession Version XM_022413729.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product extracellular calcium-sensing receptor isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006615.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATTTC ACAGCTGCTC TTTGATCCTC TTGGCAATCA CCTGGTGCAC TTCTGCCTAT 
    GGGCCTGACC AACGAGCCCA GAAGAAAGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
    CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTAAAGTCAA GGCCGGAGTC TGTGGAATGT
    ATCAGGTACA ATTTCCGCGG GTTTCGTTGG TTACAAGCAA TGATATTTGC CATCGAGGAA
    ATAAACAGCA GCCCAGCCCT TCTTCCAAAC ATGACACTGG GATACAGAAT ATTTGACACT
    TGCAACACCG TTTCTAAAGC CTTGGAGGCC ACTCTGAGTT TTGTGGCACA GAATAAAATT
    GATTCTCTGA ACCTTGACGA GTTCTGCAAC TGCTCAGAGC ATATCCCCTC TACTATCGCT
    GTGGTGGGAG CAACTGGCTC GGGCATCTCC ACGGCTGTGG CAAACCTGCT GGGCCTCTTC
    TACATCCCCC AGGTCAGCTA TGCCTCCTCC AGCAGACTCC TCAGCAATAA GAATCAGTTC
    AAGTCCTTCC TCCGTACCAT CCCCAATGAT GAACACCAGG CCACTGCCAT GGCAGACATT
    ATTGAGTATT TCCGCTGGAA CTGGGTGGGC ACCATTGCAG CTGATGATGA CTACGGCCGG
    CCAGGGATTG AGAAGTTCCG AGAGGAAGCA GAGGAGAGGG ACATCTGCAT CGACTTCAGT
    GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATTCAGC AAGTGGTAGA GGTGATCCAG
    AATTCCACAG CCAAAGTCAT TGTTGTTTTC TCCAGTGGCC CAGACCTTGA ACCCCTCATC
    AAGGAGATCG TCCGGCGAAA TATCACAGGA AGGATTTGGC TGGCCAGTGA GGCCTGGGCC
    AGCTCTTCCT TGATTGCCAT GCCCGAGTAC TTCCATGTGG TTGGAGGTAC CATTGGATTC
    GCTTTGAAGG CTGGGCAGAT CCCAGGTTTC CGGGAATTCC TGCAGAAAGT CCATCCCAGA
    AAGTCTGTCC ACAACGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
    CAAGAAGGTG CTAAAGGGCC TTTATCCATG GACACTTTCC TGAGAGGCCA CGAAGAAGGT
    GGTGGCAGGA TAAGCAACAG CTCCACTGCC TTCCGACCTC TTTGCACAGG AGATGAGAAC
    ATCAGTAGTG TGGAGACCCC TTATATGGAT TATACACACT TACGGATATC CTACAACGTC
    TACTTAGCAG TCTATTCCAT TGCTCATGCC CTGCAAGATA TATATACATG CTTACCTGGG
    AGAGGGCTCT TCACCAACGG TTCCTGTGCT GATATTAAGA AGGTTGAGGC TTGGCAGGTC
    TTGAAGCACC TACGGCACCT AAACTTTACC AACAATATGG GGGAGCAAGT GACTTTCGAT
    GAATGTGGTG ACCTGATGGG GAACTATTCC ATCATCAACT GGCACCTCTC TCCAGAGGAT
    GGCTCCATAG TGTTTAAGGA AGTCGGATAT TACAATGTCT ATGCCAAGAA AGGAGAAAGA
    CTCTTCATCA ATGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGAT GCCATTTTCC
    AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGAA TCATTGAGGG GGAGCCTACC
    TGCTGCTTTG AGTGTGTGGA GTGCCCCGAC GGGGAGTACA GTGATGAAAC AGATGCAAGT
    GCCTGTGACA AGTGCCCCGA TGACTTCTGG TCCAATGAAA ACCACACTTC GTGCATTGCC
    AAAGAGATTG AGTTTCTGTC CTGGACAGAG CCCTTTGGGA TTGCACTCAC CCTCTTTGCT
    GTGCTGGGCA TTTTCCTGAC AGCTTTCGTG CTGGGGGTCT TCATCAAGTT CCGTAACACG
    CCCATCGTCA AGGCCACCAA CCGAGAGCTC TCGTACCTCC TCCTCTTCTC CTTGCTGTGC
    TGCTTCTCCA GCTCCCTGTT CTTCATTGGC GAGCCCCAGG ACTGGACCTG CCGCCTGCGC
    CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATATCAT GCATCCTGGT GAAAACCAAC
    CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACAAGCT TCCACCGCAA GTGGTGGGGG
    CTCAACCTGC AGTTCCTGCT GGTCTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
    ATCTGGCTCT ACACGGCGCC TCCCTCCAGC TACCGCAACC ATGAGCTGGA GGACGAGATC
    ATCTTCATCA CATGCCACGA GGGCTCCCTG ATGGCCCTGG GCTTCCTGAT TGGCTACACC
    TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
    TTCAACGAGG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
    TTCATTCCAG CCTACGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTGGA AGTGATCGCC
    ATCCTGGCCG CCAGCTTTGG CCTCCTGGCC TGCATCTTCT TCAACAAGGT GTACATCATC
    CTCTTCAAGC CGTCCCGCAA CACCATCGAG GAGGTGCGCT GCAGCACCGC GGCTCACGCT
    TTCAAGGTCG CGGCCCGCGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCGGC
    AGCCTGGGGG GCTCCACGGG CTCCACGCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGT
    GAAGACCCCT TCCCGCAGCC CGAGAGGCAG AAGCAGCAGC AGCCCCTGGC CCTGACCCAG
    CGGGAGCAGC AGCCGCCGCA GCCCTTGACC TTGCCGCCGC AGCCGCAGCC CAGGTGCAAG
    CAGAAGGTCA TCTTCGGCAG TGGCACCGTC ACCTTCTCGC TGAGCTTTGA CGAGCCGCAG
    AAGAGCGCCG CGGCCCCCCG CAATTCCACG CTGCAGCACT CCCTGGAGGC CCAGCGGAGC
    CCCGAGCCCC CCGCCAGACC CCAGGCGTTA CTGCCGCCGC AGGGCGGAGA CACAGACGCG
    GAGCTGCCGG CCCAGGAGCC GGGCCTGCAG GGCCCCGGGG GTGCGGACCG CCGCCCGGAG
    ATGCGAGACC CCGAAGAGCT GTCCCCAGCC CTGGTGGTGT CCAGCTCACA AAGCTTTGTC
    ATCAGCGGCG GAGGCAGCAC GGTCACGGAA AACATACTGC ATTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269437.1
    CDS1152..4379
    Translation

    Target ORF information:

    RefSeq Version XM_022413729.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris calcium sensing receptor (CASR), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413729.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATTTC ACAGCTGCTC TTTGATCCTC TTGGCAATCA CCTGGTGCAC TTCTGCCTAT 
    GGGCCTGACC AACGAGCCCA GAAGAAAGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
    CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTAAAGTCAA GGCCGGAGTC TGTGGAATGT
    ATCAGGTACA ATTTCCGCGG GTTTCGTTGG TTACAAGCAA TGATATTTGC CATCGAGGAA
    ATAAACAGCA GCCCAGCCCT TCTTCCAAAC ATGACACTGG GATACAGAAT ATTTGACACT
    TGCAACACCG TTTCTAAAGC CTTGGAGGCC ACTCTGAGTT TTGTGGCACA GAATAAAATT
    GATTCTCTGA ACCTTGACGA GTTCTGCAAC TGCTCAGAGC ATATCCCCTC TACTATCGCT
    GTGGTGGGAG CAACTGGCTC GGGCATCTCC ACGGCTGTGG CAAACCTGCT GGGCCTCTTC
    TACATCCCCC AGGTCAGCTA TGCCTCCTCC AGCAGACTCC TCAGCAATAA GAATCAGTTC
    AAGTCCTTCC TCCGTACCAT CCCCAATGAT GAACACCAGG CCACTGCCAT GGCAGACATT
    ATTGAGTATT TCCGCTGGAA CTGGGTGGGC ACCATTGCAG CTGATGATGA CTACGGCCGG
    CCAGGGATTG AGAAGTTCCG AGAGGAAGCA GAGGAGAGGG ACATCTGCAT CGACTTCAGT
    GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATTCAGC AAGTGGTAGA GGTGATCCAG
    AATTCCACAG CCAAAGTCAT TGTTGTTTTC TCCAGTGGCC CAGACCTTGA ACCCCTCATC
    AAGGAGATCG TCCGGCGAAA TATCACAGGA AGGATTTGGC TGGCCAGTGA GGCCTGGGCC
    AGCTCTTCCT TGATTGCCAT GCCCGAGTAC TTCCATGTGG TTGGAGGTAC CATTGGATTC
    GCTTTGAAGG CTGGGCAGAT CCCAGGTTTC CGGGAATTCC TGCAGAAAGT CCATCCCAGA
    AAGTCTGTCC ACAACGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
    CAAGAAGGTG CTAAAGGGCC TTTATCCATG GACACTTTCC TGAGAGGCCA CGAAGAAGGT
    GGTGGCAGGA TAAGCAACAG CTCCACTGCC TTCCGACCTC TTTGCACAGG AGATGAGAAC
    ATCAGTAGTG TGGAGACCCC TTATATGGAT TATACACACT TACGGATATC CTACAACGTC
    TACTTAGCAG TCTATTCCAT TGCTCATGCC CTGCAAGATA TATATACATG CTTACCTGGG
    AGAGGGCTCT TCACCAACGG TTCCTGTGCT GATATTAAGA AGGTTGAGGC TTGGCAGGTC
    TTGAAGCACC TACGGCACCT AAACTTTACC AACAATATGG GGGAGCAAGT GACTTTCGAT
    GAATGTGGTG ACCTGATGGG GAACTATTCC ATCATCAACT GGCACCTCTC TCCAGAGGAT
    GGCTCCATAG TGTTTAAGGA AGTCGGATAT TACAATGTCT ATGCCAAGAA AGGAGAAAGA
    CTCTTCATCA ATGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGAT GCCATTTTCC
    AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGAA TCATTGAGGG GGAGCCTACC
    TGCTGCTTTG AGTGTGTGGA GTGCCCCGAC GGGGAGTACA GTGATGAAAC AGATGCAAGT
    GCCTGTGACA AGTGCCCCGA TGACTTCTGG TCCAATGAAA ACCACACTTC GTGCATTGCC
    AAAGAGATTG AGTTTCTGTC CTGGACAGAG CCCTTTGGGA TTGCACTCAC CCTCTTTGCT
    GTGCTGGGCA TTTTCCTGAC AGCTTTCGTG CTGGGGGTCT TCATCAAGTT CCGTAACACG
    CCCATCGTCA AGGCCACCAA CCGAGAGCTC TCGTACCTCC TCCTCTTCTC CTTGCTGTGC
    TGCTTCTCCA GCTCCCTGTT CTTCATTGGC GAGCCCCAGG ACTGGACCTG CCGCCTGCGC
    CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATATCAT GCATCCTGGT GAAAACCAAC
    CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACAAGCT TCCACCGCAA GTGGTGGGGG
    CTCAACCTGC AGTTCCTGCT GGTCTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
    ATCTGGCTCT ACACGGCGCC TCCCTCCAGC TACCGCAACC ATGAGCTGGA GGACGAGATC
    ATCTTCATCA CATGCCACGA GGGCTCCCTG ATGGCCCTGG GCTTCCTGAT TGGCTACACC
    TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
    TTCAACGAGG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
    TTCATTCCAG CCTACGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTGGA AGTGATCGCC
    ATCCTGGCCG CCAGCTTTGG CCTCCTGGCC TGCATCTTCT TCAACAAGGT GTACATCATC
    CTCTTCAAGC CGTCCCGCAA CACCATCGAG GAGGTGCGCT GCAGCACCGC GGCTCACGCT
    TTCAAGGTCG CGGCCCGCGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCGGC
    AGCCTGGGGG GCTCCACGGG CTCCACGCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGT
    GAAGACCCCT TCCCGCAGCC CGAGAGGCAG AAGCAGCAGC AGCCCCTGGC CCTGACCCAG
    CGGGAGCAGC AGCCGCCGCA GCCCTTGACC TTGCCGCCGC AGCCGCAGCC CAGGTGCAAG
    CAGAAGGTCA TCTTCGGCAG TGGCACCGTC ACCTTCTCGC TGAGCTTTGA CGAGCCGCAG
    AAGAGCGCCG CGGCCCCCCG CAATTCCACG CTGCAGCACT CCCTGGAGGC CCAGCGGAGC
    CCCGAGCCCC CCGCCAGACC CCAGGCGTTA CTGCCGCCGC AGGGCGGAGA CACAGACGCG
    GAGCTGCCGG CCCAGGAGCC GGGCCTGCAG GGCCCCGGGG GTGCGGACCG CCGCCCGGAG
    ATGCGAGACC CCGAAGAGCT GTCCCCAGCC CTGGTGGTGT CCAGCTCACA AAGCTTTGTC
    ATCAGCGGCG GAGGCAGCAC GGTCACGGAA AACATACTGC ATTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30072
    Clone ID Related Accession (Same CDS sequence) XM_014110142.1 , NM_001081509.2 , XM_014110141.2 , XM_014110143.2 , XM_022413728.1 , XM_014110143.1 , XM_022413727.1 , XM_022413729.1 , XM_014110141.1 , XM_022413726.1 , XM_014110142.2
    Accession Version XM_014110141.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product extracellular calcium-sensing receptor isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726118.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATTTC ACAGCTGCTC TTTGATCCTC TTGGCAATCA CCTGGTGCAC TTCTGCCTAT 
    GGGCCTGACC AACGAGCCCA GAAGAAAGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
    CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTAAAGTCAA GGCCGGAGTC TGTGGAATGT
    ATCAGGTACA ATTTCCGCGG GTTTCGTTGG TTACAAGCAA TGATATTTGC CATCGAGGAA
    ATAAACAGCA GCCCAGCCCT TCTTCCAAAC ATGACACTGG GATACAGAAT ATTTGACACT
    TGCAACACCG TTTCTAAAGC CTTGGAGGCC ACTCTGAGTT TTGTGGCACA GAATAAAATT
    GATTCTCTGA ACCTTGACGA GTTCTGCAAC TGCTCAGAGC ATATCCCCTC TACTATCGCT
    GTGGTGGGAG CAACTGGCTC GGGCATCTCC ACGGCTGTGG CAAACCTGCT GGGCCTCTTC
    TACATCCCCC AGGTCAGCTA TGCCTCCTCC AGCAGACTCC TCAGCAATAA GAATCAGTTC
    AAGTCCTTCC TCCGTACCAT CCCCAATGAT GAACACCAGG CCACTGCCAT GGCAGACATT
    ATTGAGTATT TCCGCTGGAA CTGGGTGGGC ACCATTGCAG CTGATGATGA CTACGGCCGG
    CCAGGGATTG AGAAGTTCCG AGAGGAAGCA GAGGAGAGGG ACATCTGCAT CGACTTCAGT
    GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATTCAGC AAGTGGTAGA GGTGATCCAG
    AATTCCACAG CCAAAGTCAT TGTTGTTTTC TCCAGTGGCC CAGACCTTGA ACCCCTCATC
    AAGGAGATCG TCCGGCGAAA TATCACAGGA AGGATTTGGC TGGCCAGTGA GGCCTGGGCC
    AGCTCTTCCT TGATTGCCAT GCCCGAGTAC TTCCATGTGG TTGGAGGTAC CATTGGATTC
    GCTTTGAAGG CTGGGCAGAT CCCAGGTTTC CGGGAATTCC TGCAGAAAGT CCATCCCAGA
    AAGTCTGTCC ACAACGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
    CAAGAAGGTG CTAAAGGGCC TTTATCCATG GACACTTTCC TGAGAGGCCA CGAAGAAGGT
    GGTGGCAGGA TAAGCAACAG CTCCACTGCC TTCCGACCTC TTTGCACAGG AGATGAGAAC
    ATCAGTAGTG TGGAGACCCC TTATATGGAT TATACACACT TACGGATATC CTACAACGTC
    TACTTAGCAG TCTATTCCAT TGCTCATGCC CTGCAAGATA TATATACATG CTTACCTGGG
    AGAGGGCTCT TCACCAACGG TTCCTGTGCT GATATTAAGA AGGTTGAGGC TTGGCAGGTC
    TTGAAGCACC TACGGCACCT AAACTTTACC AACAATATGG GGGAGCAAGT GACTTTCGAT
    GAATGTGGTG ACCTGATGGG GAACTATTCC ATCATCAACT GGCACCTCTC TCCAGAGGAT
    GGCTCCATAG TGTTTAAGGA AGTCGGATAT TACAATGTCT ATGCCAAGAA AGGAGAAAGA
    CTCTTCATCA ATGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGAT GCCATTTTCC
    AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGAA TCATTGAGGG GGAGCCTACC
    TGCTGCTTTG AGTGTGTGGA GTGCCCCGAC GGGGAGTACA GTGATGAAAC AGATGCAAGT
    GCCTGTGACA AGTGCCCCGA TGACTTCTGG TCCAATGAAA ACCACACTTC GTGCATTGCC
    AAAGAGATTG AGTTTCTGTC CTGGACAGAG CCCTTTGGGA TTGCACTCAC CCTCTTTGCT
    GTGCTGGGCA TTTTCCTGAC AGCTTTCGTG CTGGGGGTCT TCATCAAGTT CCGTAACACG
    CCCATCGTCA AGGCCACCAA CCGAGAGCTC TCGTACCTCC TCCTCTTCTC CTTGCTGTGC
    TGCTTCTCCA GCTCCCTGTT CTTCATTGGC GAGCCCCAGG ACTGGACCTG CCGCCTGCGC
    CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATATCAT GCATCCTGGT GAAAACCAAC
    CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACAAGCT TCCACCGCAA GTGGTGGGGG
    CTCAACCTGC AGTTCCTGCT GGTCTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
    ATCTGGCTCT ACACGGCGCC TCCCTCCAGC TACCGCAACC ATGAGCTGGA GGACGAGATC
    ATCTTCATCA CATGCCACGA GGGCTCCCTG ATGGCCCTGG GCTTCCTGAT TGGCTACACC
    TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
    TTCAACGAGG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
    TTCATTCCAG CCTACGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTGGA AGTGATCGCC
    ATCCTGGCCG CCAGCTTTGG CCTCCTGGCC TGCATCTTCT TCAACAAGGT GTACATCATC
    CTCTTCAAGC CGTCCCGCAA CACCATCGAG GAGGTGCGCT GCAGCACCGC GGCTCACGCT
    TTCAAGGTCG CGGCCCGCGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCGGC
    AGCCTGGGGG GCTCCACGGG CTCCACGCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGT
    GAAGACCCCT TCCCGCAGCC CGAGAGGCAG AAGCAGCAGC AGCCCCTGGC CCTGACCCAG
    CGGGAGCAGC AGCCGCCGCA GCCCTTGACC TTGCCGCCGC AGCCGCAGCC CAGGTGCAAG
    CAGAAGGTCA TCTTCGGCAG TGGCACCGTC ACCTTCTCGC TGAGCTTTGA CGAGCCGCAG
    AAGAGCGCCG CGGCCCCCCG CAATTCCACG CTGCAGCACT CCCTGGAGGC CCAGCGGAGC
    CCCGAGCCCC CCGCCAGACC CCAGGCGTTA CTGCCGCCGC AGGGCGGAGA CACAGACGCG
    GAGCTGCCGG CCCAGGAGCC GGGCCTGCAG GGCCCCGGGG GTGCGGACCG CCGCCCGGAG
    ATGCGAGACC CCGAAGAGCT GTCCCCAGCC CTGGTGGTGT CCAGCTCACA AAGCTTTGTC
    ATCAGCGGCG GAGGCAGCAC GGTCACGGAA AACATACTGC ATTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965616.1
    CDS776..4003
    Translation

    Target ORF information:

    RefSeq Version XM_014110141.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris calcium-sensing receptor (CASR), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110141.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATTTC ACAGCTGCTC TTTGATCCTC TTGGCAATCA CCTGGTGCAC TTCTGCCTAT 
    GGGCCTGACC AACGAGCCCA GAAGAAAGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
    CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTAAAGTCAA GGCCGGAGTC TGTGGAATGT
    ATCAGGTACA ATTTCCGCGG GTTTCGTTGG TTACAAGCAA TGATATTTGC CATCGAGGAA
    ATAAACAGCA GCCCAGCCCT TCTTCCAAAC ATGACACTGG GATACAGAAT ATTTGACACT
    TGCAACACCG TTTCTAAAGC CTTGGAGGCC ACTCTGAGTT TTGTGGCACA GAATAAAATT
    GATTCTCTGA ACCTTGACGA GTTCTGCAAC TGCTCAGAGC ATATCCCCTC TACTATCGCT
    GTGGTGGGAG CAACTGGCTC GGGCATCTCC ACGGCTGTGG CAAACCTGCT GGGCCTCTTC
    TACATCCCCC AGGTCAGCTA TGCCTCCTCC AGCAGACTCC TCAGCAATAA GAATCAGTTC
    AAGTCCTTCC TCCGTACCAT CCCCAATGAT GAACACCAGG CCACTGCCAT GGCAGACATT
    ATTGAGTATT TCCGCTGGAA CTGGGTGGGC ACCATTGCAG CTGATGATGA CTACGGCCGG
    CCAGGGATTG AGAAGTTCCG AGAGGAAGCA GAGGAGAGGG ACATCTGCAT CGACTTCAGT
    GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATTCAGC AAGTGGTAGA GGTGATCCAG
    AATTCCACAG CCAAAGTCAT TGTTGTTTTC TCCAGTGGCC CAGACCTTGA ACCCCTCATC
    AAGGAGATCG TCCGGCGAAA TATCACAGGA AGGATTTGGC TGGCCAGTGA GGCCTGGGCC
    AGCTCTTCCT TGATTGCCAT GCCCGAGTAC TTCCATGTGG TTGGAGGTAC CATTGGATTC
    GCTTTGAAGG CTGGGCAGAT CCCAGGTTTC CGGGAATTCC TGCAGAAAGT CCATCCCAGA
    AAGTCTGTCC ACAACGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
    CAAGAAGGTG CTAAAGGGCC TTTATCCATG GACACTTTCC TGAGAGGCCA CGAAGAAGGT
    GGTGGCAGGA TAAGCAACAG CTCCACTGCC TTCCGACCTC TTTGCACAGG AGATGAGAAC
    ATCAGTAGTG TGGAGACCCC TTATATGGAT TATACACACT TACGGATATC CTACAACGTC
    TACTTAGCAG TCTATTCCAT TGCTCATGCC CTGCAAGATA TATATACATG CTTACCTGGG
    AGAGGGCTCT TCACCAACGG TTCCTGTGCT GATATTAAGA AGGTTGAGGC TTGGCAGGTC
    TTGAAGCACC TACGGCACCT AAACTTTACC AACAATATGG GGGAGCAAGT GACTTTCGAT
    GAATGTGGTG ACCTGATGGG GAACTATTCC ATCATCAACT GGCACCTCTC TCCAGAGGAT
    GGCTCCATAG TGTTTAAGGA AGTCGGATAT TACAATGTCT ATGCCAAGAA AGGAGAAAGA
    CTCTTCATCA ATGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGAT GCCATTTTCC
    AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGAA TCATTGAGGG GGAGCCTACC
    TGCTGCTTTG AGTGTGTGGA GTGCCCCGAC GGGGAGTACA GTGATGAAAC AGATGCAAGT
    GCCTGTGACA AGTGCCCCGA TGACTTCTGG TCCAATGAAA ACCACACTTC GTGCATTGCC
    AAAGAGATTG AGTTTCTGTC CTGGACAGAG CCCTTTGGGA TTGCACTCAC CCTCTTTGCT
    GTGCTGGGCA TTTTCCTGAC AGCTTTCGTG CTGGGGGTCT TCATCAAGTT CCGTAACACG
    CCCATCGTCA AGGCCACCAA CCGAGAGCTC TCGTACCTCC TCCTCTTCTC CTTGCTGTGC
    TGCTTCTCCA GCTCCCTGTT CTTCATTGGC GAGCCCCAGG ACTGGACCTG CCGCCTGCGC
    CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATATCAT GCATCCTGGT GAAAACCAAC
    CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACAAGCT TCCACCGCAA GTGGTGGGGG
    CTCAACCTGC AGTTCCTGCT GGTCTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
    ATCTGGCTCT ACACGGCGCC TCCCTCCAGC TACCGCAACC ATGAGCTGGA GGACGAGATC
    ATCTTCATCA CATGCCACGA GGGCTCCCTG ATGGCCCTGG GCTTCCTGAT TGGCTACACC
    TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
    TTCAACGAGG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
    TTCATTCCAG CCTACGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTGGA AGTGATCGCC
    ATCCTGGCCG CCAGCTTTGG CCTCCTGGCC TGCATCTTCT TCAACAAGGT GTACATCATC
    CTCTTCAAGC CGTCCCGCAA CACCATCGAG GAGGTGCGCT GCAGCACCGC GGCTCACGCT
    TTCAAGGTCG CGGCCCGCGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCGGC
    AGCCTGGGGG GCTCCACGGG CTCCACGCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGT
    GAAGACCCCT TCCCGCAGCC CGAGAGGCAG AAGCAGCAGC AGCCCCTGGC CCTGACCCAG
    CGGGAGCAGC AGCCGCCGCA GCCCTTGACC TTGCCGCCGC AGCCGCAGCC CAGGTGCAAG
    CAGAAGGTCA TCTTCGGCAG TGGCACCGTC ACCTTCTCGC TGAGCTTTGA CGAGCCGCAG
    AAGAGCGCCG CGGCCCCCCG CAATTCCACG CTGCAGCACT CCCTGGAGGC CCAGCGGAGC
    CCCGAGCCCC CCGCCAGACC CCAGGCGTTA CTGCCGCCGC AGGGCGGAGA CACAGACGCG
    GAGCTGCCGG CCCAGGAGCC GGGCCTGCAG GGCCCCGGGG GTGCGGACCG CCGCCCGGAG
    ATGCGAGACC CCGAAGAGCT GTCCCCAGCC CTGGTGGTGT CCAGCTCACA AAGCTTTGTC
    ATCAGCGGCG GAGGCAGCAC GGTCACGGAA AACATACTGC ATTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30072
    Clone ID Related Accession (Same CDS sequence) XM_014110142.1 , NM_001081509.2 , XM_014110141.2 , XM_014110143.2 , XM_022413728.1 , XM_014110143.1 , XM_022413727.1 , XM_022413729.1 , XM_014110141.1 , XM_022413726.1 , XM_014110142.2
    Accession Version XM_022413726.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product extracellular calcium-sensing receptor isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006615.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATTTC ACAGCTGCTC TTTGATCCTC TTGGCAATCA CCTGGTGCAC TTCTGCCTAT 
    GGGCCTGACC AACGAGCCCA GAAGAAAGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
    CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTAAAGTCAA GGCCGGAGTC TGTGGAATGT
    ATCAGGTACA ATTTCCGCGG GTTTCGTTGG TTACAAGCAA TGATATTTGC CATCGAGGAA
    ATAAACAGCA GCCCAGCCCT TCTTCCAAAC ATGACACTGG GATACAGAAT ATTTGACACT
    TGCAACACCG TTTCTAAAGC CTTGGAGGCC ACTCTGAGTT TTGTGGCACA GAATAAAATT
    GATTCTCTGA ACCTTGACGA GTTCTGCAAC TGCTCAGAGC ATATCCCCTC TACTATCGCT
    GTGGTGGGAG CAACTGGCTC GGGCATCTCC ACGGCTGTGG CAAACCTGCT GGGCCTCTTC
    TACATCCCCC AGGTCAGCTA TGCCTCCTCC AGCAGACTCC TCAGCAATAA GAATCAGTTC
    AAGTCCTTCC TCCGTACCAT CCCCAATGAT GAACACCAGG CCACTGCCAT GGCAGACATT
    ATTGAGTATT TCCGCTGGAA CTGGGTGGGC ACCATTGCAG CTGATGATGA CTACGGCCGG
    CCAGGGATTG AGAAGTTCCG AGAGGAAGCA GAGGAGAGGG ACATCTGCAT CGACTTCAGT
    GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATTCAGC AAGTGGTAGA GGTGATCCAG
    AATTCCACAG CCAAAGTCAT TGTTGTTTTC TCCAGTGGCC CAGACCTTGA ACCCCTCATC
    AAGGAGATCG TCCGGCGAAA TATCACAGGA AGGATTTGGC TGGCCAGTGA GGCCTGGGCC
    AGCTCTTCCT TGATTGCCAT GCCCGAGTAC TTCCATGTGG TTGGAGGTAC CATTGGATTC
    GCTTTGAAGG CTGGGCAGAT CCCAGGTTTC CGGGAATTCC TGCAGAAAGT CCATCCCAGA
    AAGTCTGTCC ACAACGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
    CAAGAAGGTG CTAAAGGGCC TTTATCCATG GACACTTTCC TGAGAGGCCA CGAAGAAGGT
    GGTGGCAGGA TAAGCAACAG CTCCACTGCC TTCCGACCTC TTTGCACAGG AGATGAGAAC
    ATCAGTAGTG TGGAGACCCC TTATATGGAT TATACACACT TACGGATATC CTACAACGTC
    TACTTAGCAG TCTATTCCAT TGCTCATGCC CTGCAAGATA TATATACATG CTTACCTGGG
    AGAGGGCTCT TCACCAACGG TTCCTGTGCT GATATTAAGA AGGTTGAGGC TTGGCAGGTC
    TTGAAGCACC TACGGCACCT AAACTTTACC AACAATATGG GGGAGCAAGT GACTTTCGAT
    GAATGTGGTG ACCTGATGGG GAACTATTCC ATCATCAACT GGCACCTCTC TCCAGAGGAT
    GGCTCCATAG TGTTTAAGGA AGTCGGATAT TACAATGTCT ATGCCAAGAA AGGAGAAAGA
    CTCTTCATCA ATGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGAT GCCATTTTCC
    AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGAA TCATTGAGGG GGAGCCTACC
    TGCTGCTTTG AGTGTGTGGA GTGCCCCGAC GGGGAGTACA GTGATGAAAC AGATGCAAGT
    GCCTGTGACA AGTGCCCCGA TGACTTCTGG TCCAATGAAA ACCACACTTC GTGCATTGCC
    AAAGAGATTG AGTTTCTGTC CTGGACAGAG CCCTTTGGGA TTGCACTCAC CCTCTTTGCT
    GTGCTGGGCA TTTTCCTGAC AGCTTTCGTG CTGGGGGTCT TCATCAAGTT CCGTAACACG
    CCCATCGTCA AGGCCACCAA CCGAGAGCTC TCGTACCTCC TCCTCTTCTC CTTGCTGTGC
    TGCTTCTCCA GCTCCCTGTT CTTCATTGGC GAGCCCCAGG ACTGGACCTG CCGCCTGCGC
    CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATATCAT GCATCCTGGT GAAAACCAAC
    CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACAAGCT TCCACCGCAA GTGGTGGGGG
    CTCAACCTGC AGTTCCTGCT GGTCTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
    ATCTGGCTCT ACACGGCGCC TCCCTCCAGC TACCGCAACC ATGAGCTGGA GGACGAGATC
    ATCTTCATCA CATGCCACGA GGGCTCCCTG ATGGCCCTGG GCTTCCTGAT TGGCTACACC
    TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
    TTCAACGAGG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
    TTCATTCCAG CCTACGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTGGA AGTGATCGCC
    ATCCTGGCCG CCAGCTTTGG CCTCCTGGCC TGCATCTTCT TCAACAAGGT GTACATCATC
    CTCTTCAAGC CGTCCCGCAA CACCATCGAG GAGGTGCGCT GCAGCACCGC GGCTCACGCT
    TTCAAGGTCG CGGCCCGCGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCGGC
    AGCCTGGGGG GCTCCACGGG CTCCACGCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGT
    GAAGACCCCT TCCCGCAGCC CGAGAGGCAG AAGCAGCAGC AGCCCCTGGC CCTGACCCAG
    CGGGAGCAGC AGCCGCCGCA GCCCTTGACC TTGCCGCCGC AGCCGCAGCC CAGGTGCAAG
    CAGAAGGTCA TCTTCGGCAG TGGCACCGTC ACCTTCTCGC TGAGCTTTGA CGAGCCGCAG
    AAGAGCGCCG CGGCCCCCCG CAATTCCACG CTGCAGCACT CCCTGGAGGC CCAGCGGAGC
    CCCGAGCCCC CCGCCAGACC CCAGGCGTTA CTGCCGCCGC AGGGCGGAGA CACAGACGCG
    GAGCTGCCGG CCCAGGAGCC GGGCCTGCAG GGCCCCGGGG GTGCGGACCG CCGCCCGGAG
    ATGCGAGACC CCGAAGAGCT GTCCCCAGCC CTGGTGGTGT CCAGCTCACA AAGCTTTGTC
    ATCAGCGGCG GAGGCAGCAC GGTCACGGAA AACATACTGC ATTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269434.1
    CDS731..3958
    Translation

    Target ORF information:

    RefSeq Version XM_022413726.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris calcium sensing receptor (CASR), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413726.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATTTC ACAGCTGCTC TTTGATCCTC TTGGCAATCA CCTGGTGCAC TTCTGCCTAT 
    GGGCCTGACC AACGAGCCCA GAAGAAAGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
    CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTAAAGTCAA GGCCGGAGTC TGTGGAATGT
    ATCAGGTACA ATTTCCGCGG GTTTCGTTGG TTACAAGCAA TGATATTTGC CATCGAGGAA
    ATAAACAGCA GCCCAGCCCT TCTTCCAAAC ATGACACTGG GATACAGAAT ATTTGACACT
    TGCAACACCG TTTCTAAAGC CTTGGAGGCC ACTCTGAGTT TTGTGGCACA GAATAAAATT
    GATTCTCTGA ACCTTGACGA GTTCTGCAAC TGCTCAGAGC ATATCCCCTC TACTATCGCT
    GTGGTGGGAG CAACTGGCTC GGGCATCTCC ACGGCTGTGG CAAACCTGCT GGGCCTCTTC
    TACATCCCCC AGGTCAGCTA TGCCTCCTCC AGCAGACTCC TCAGCAATAA GAATCAGTTC
    AAGTCCTTCC TCCGTACCAT CCCCAATGAT GAACACCAGG CCACTGCCAT GGCAGACATT
    ATTGAGTATT TCCGCTGGAA CTGGGTGGGC ACCATTGCAG CTGATGATGA CTACGGCCGG
    CCAGGGATTG AGAAGTTCCG AGAGGAAGCA GAGGAGAGGG ACATCTGCAT CGACTTCAGT
    GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATTCAGC AAGTGGTAGA GGTGATCCAG
    AATTCCACAG CCAAAGTCAT TGTTGTTTTC TCCAGTGGCC CAGACCTTGA ACCCCTCATC
    AAGGAGATCG TCCGGCGAAA TATCACAGGA AGGATTTGGC TGGCCAGTGA GGCCTGGGCC
    AGCTCTTCCT TGATTGCCAT GCCCGAGTAC TTCCATGTGG TTGGAGGTAC CATTGGATTC
    GCTTTGAAGG CTGGGCAGAT CCCAGGTTTC CGGGAATTCC TGCAGAAAGT CCATCCCAGA
    AAGTCTGTCC ACAACGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
    CAAGAAGGTG CTAAAGGGCC TTTATCCATG GACACTTTCC TGAGAGGCCA CGAAGAAGGT
    GGTGGCAGGA TAAGCAACAG CTCCACTGCC TTCCGACCTC TTTGCACAGG AGATGAGAAC
    ATCAGTAGTG TGGAGACCCC TTATATGGAT TATACACACT TACGGATATC CTACAACGTC
    TACTTAGCAG TCTATTCCAT TGCTCATGCC CTGCAAGATA TATATACATG CTTACCTGGG
    AGAGGGCTCT TCACCAACGG TTCCTGTGCT GATATTAAGA AGGTTGAGGC TTGGCAGGTC
    TTGAAGCACC TACGGCACCT AAACTTTACC AACAATATGG GGGAGCAAGT GACTTTCGAT
    GAATGTGGTG ACCTGATGGG GAACTATTCC ATCATCAACT GGCACCTCTC TCCAGAGGAT
    GGCTCCATAG TGTTTAAGGA AGTCGGATAT TACAATGTCT ATGCCAAGAA AGGAGAAAGA
    CTCTTCATCA ATGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGAT GCCATTTTCC
    AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGAA TCATTGAGGG GGAGCCTACC
    TGCTGCTTTG AGTGTGTGGA GTGCCCCGAC GGGGAGTACA GTGATGAAAC AGATGCAAGT
    GCCTGTGACA AGTGCCCCGA TGACTTCTGG TCCAATGAAA ACCACACTTC GTGCATTGCC
    AAAGAGATTG AGTTTCTGTC CTGGACAGAG CCCTTTGGGA TTGCACTCAC CCTCTTTGCT
    GTGCTGGGCA TTTTCCTGAC AGCTTTCGTG CTGGGGGTCT TCATCAAGTT CCGTAACACG
    CCCATCGTCA AGGCCACCAA CCGAGAGCTC TCGTACCTCC TCCTCTTCTC CTTGCTGTGC
    TGCTTCTCCA GCTCCCTGTT CTTCATTGGC GAGCCCCAGG ACTGGACCTG CCGCCTGCGC
    CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATATCAT GCATCCTGGT GAAAACCAAC
    CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACAAGCT TCCACCGCAA GTGGTGGGGG
    CTCAACCTGC AGTTCCTGCT GGTCTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
    ATCTGGCTCT ACACGGCGCC TCCCTCCAGC TACCGCAACC ATGAGCTGGA GGACGAGATC
    ATCTTCATCA CATGCCACGA GGGCTCCCTG ATGGCCCTGG GCTTCCTGAT TGGCTACACC
    TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
    TTCAACGAGG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
    TTCATTCCAG CCTACGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTGGA AGTGATCGCC
    ATCCTGGCCG CCAGCTTTGG CCTCCTGGCC TGCATCTTCT TCAACAAGGT GTACATCATC
    CTCTTCAAGC CGTCCCGCAA CACCATCGAG GAGGTGCGCT GCAGCACCGC GGCTCACGCT
    TTCAAGGTCG CGGCCCGCGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCGGC
    AGCCTGGGGG GCTCCACGGG CTCCACGCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGT
    GAAGACCCCT TCCCGCAGCC CGAGAGGCAG AAGCAGCAGC AGCCCCTGGC CCTGACCCAG
    CGGGAGCAGC AGCCGCCGCA GCCCTTGACC TTGCCGCCGC AGCCGCAGCC CAGGTGCAAG
    CAGAAGGTCA TCTTCGGCAG TGGCACCGTC ACCTTCTCGC TGAGCTTTGA CGAGCCGCAG
    AAGAGCGCCG CGGCCCCCCG CAATTCCACG CTGCAGCACT CCCTGGAGGC CCAGCGGAGC
    CCCGAGCCCC CCGCCAGACC CCAGGCGTTA CTGCCGCCGC AGGGCGGAGA CACAGACGCG
    GAGCTGCCGG CCCAGGAGCC GGGCCTGCAG GGCCCCGGGG GTGCGGACCG CCGCCCGGAG
    ATGCGAGACC CCGAAGAGCT GTCCCCAGCC CTGGTGGTGT CCAGCTCACA AAGCTTTGTC
    ATCAGCGGCG GAGGCAGCAC GGTCACGGAA AACATACTGC ATTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30072
    Clone ID Related Accession (Same CDS sequence) XM_014110142.1 , NM_001081509.2 , XM_014110141.2 , XM_014110143.2 , XM_022413728.1 , XM_014110143.1 , XM_022413727.1 , XM_022413729.1 , XM_014110141.1 , XM_022413726.1 , XM_014110142.2
    Accession Version XM_014110142.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product extracellular calcium-sensing receptor isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006615.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014110142.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATTTC ACAGCTGCTC TTTGATCCTC TTGGCAATCA CCTGGTGCAC TTCTGCCTAT 
    GGGCCTGACC AACGAGCCCA GAAGAAAGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
    CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTAAAGTCAA GGCCGGAGTC TGTGGAATGT
    ATCAGGTACA ATTTCCGCGG GTTTCGTTGG TTACAAGCAA TGATATTTGC CATCGAGGAA
    ATAAACAGCA GCCCAGCCCT TCTTCCAAAC ATGACACTGG GATACAGAAT ATTTGACACT
    TGCAACACCG TTTCTAAAGC CTTGGAGGCC ACTCTGAGTT TTGTGGCACA GAATAAAATT
    GATTCTCTGA ACCTTGACGA GTTCTGCAAC TGCTCAGAGC ATATCCCCTC TACTATCGCT
    GTGGTGGGAG CAACTGGCTC GGGCATCTCC ACGGCTGTGG CAAACCTGCT GGGCCTCTTC
    TACATCCCCC AGGTCAGCTA TGCCTCCTCC AGCAGACTCC TCAGCAATAA GAATCAGTTC
    AAGTCCTTCC TCCGTACCAT CCCCAATGAT GAACACCAGG CCACTGCCAT GGCAGACATT
    ATTGAGTATT TCCGCTGGAA CTGGGTGGGC ACCATTGCAG CTGATGATGA CTACGGCCGG
    CCAGGGATTG AGAAGTTCCG AGAGGAAGCA GAGGAGAGGG ACATCTGCAT CGACTTCAGT
    GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATTCAGC AAGTGGTAGA GGTGATCCAG
    AATTCCACAG CCAAAGTCAT TGTTGTTTTC TCCAGTGGCC CAGACCTTGA ACCCCTCATC
    AAGGAGATCG TCCGGCGAAA TATCACAGGA AGGATTTGGC TGGCCAGTGA GGCCTGGGCC
    AGCTCTTCCT TGATTGCCAT GCCCGAGTAC TTCCATGTGG TTGGAGGTAC CATTGGATTC
    GCTTTGAAGG CTGGGCAGAT CCCAGGTTTC CGGGAATTCC TGCAGAAAGT CCATCCCAGA
    AAGTCTGTCC ACAACGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
    CAAGAAGGTG CTAAAGGGCC TTTATCCATG GACACTTTCC TGAGAGGCCA CGAAGAAGGT
    GGTGGCAGGA TAAGCAACAG CTCCACTGCC TTCCGACCTC TTTGCACAGG AGATGAGAAC
    ATCAGTAGTG TGGAGACCCC TTATATGGAT TATACACACT TACGGATATC CTACAACGTC
    TACTTAGCAG TCTATTCCAT TGCTCATGCC CTGCAAGATA TATATACATG CTTACCTGGG
    AGAGGGCTCT TCACCAACGG TTCCTGTGCT GATATTAAGA AGGTTGAGGC TTGGCAGGTC
    TTGAAGCACC TACGGCACCT AAACTTTACC AACAATATGG GGGAGCAAGT GACTTTCGAT
    GAATGTGGTG ACCTGATGGG GAACTATTCC ATCATCAACT GGCACCTCTC TCCAGAGGAT
    GGCTCCATAG TGTTTAAGGA AGTCGGATAT TACAATGTCT ATGCCAAGAA AGGAGAAAGA
    CTCTTCATCA ATGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGAT GCCATTTTCC
    AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGAA TCATTGAGGG GGAGCCTACC
    TGCTGCTTTG AGTGTGTGGA GTGCCCCGAC GGGGAGTACA GTGATGAAAC AGATGCAAGT
    GCCTGTGACA AGTGCCCCGA TGACTTCTGG TCCAATGAAA ACCACACTTC GTGCATTGCC
    AAAGAGATTG AGTTTCTGTC CTGGACAGAG CCCTTTGGGA TTGCACTCAC CCTCTTTGCT
    GTGCTGGGCA TTTTCCTGAC AGCTTTCGTG CTGGGGGTCT TCATCAAGTT CCGTAACACG
    CCCATCGTCA AGGCCACCAA CCGAGAGCTC TCGTACCTCC TCCTCTTCTC CTTGCTGTGC
    TGCTTCTCCA GCTCCCTGTT CTTCATTGGC GAGCCCCAGG ACTGGACCTG CCGCCTGCGC
    CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATATCAT GCATCCTGGT GAAAACCAAC
    CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACAAGCT TCCACCGCAA GTGGTGGGGG
    CTCAACCTGC AGTTCCTGCT GGTCTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
    ATCTGGCTCT ACACGGCGCC TCCCTCCAGC TACCGCAACC ATGAGCTGGA GGACGAGATC
    ATCTTCATCA CATGCCACGA GGGCTCCCTG ATGGCCCTGG GCTTCCTGAT TGGCTACACC
    TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
    TTCAACGAGG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
    TTCATTCCAG CCTACGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTGGA AGTGATCGCC
    ATCCTGGCCG CCAGCTTTGG CCTCCTGGCC TGCATCTTCT TCAACAAGGT GTACATCATC
    CTCTTCAAGC CGTCCCGCAA CACCATCGAG GAGGTGCGCT GCAGCACCGC GGCTCACGCT
    TTCAAGGTCG CGGCCCGCGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCGGC
    AGCCTGGGGG GCTCCACGGG CTCCACGCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGT
    GAAGACCCCT TCCCGCAGCC CGAGAGGCAG AAGCAGCAGC AGCCCCTGGC CCTGACCCAG
    CGGGAGCAGC AGCCGCCGCA GCCCTTGACC TTGCCGCCGC AGCCGCAGCC CAGGTGCAAG
    CAGAAGGTCA TCTTCGGCAG TGGCACCGTC ACCTTCTCGC TGAGCTTTGA CGAGCCGCAG
    AAGAGCGCCG CGGCCCCCCG CAATTCCACG CTGCAGCACT CCCTGGAGGC CCAGCGGAGC
    CCCGAGCCCC CCGCCAGACC CCAGGCGTTA CTGCCGCCGC AGGGCGGAGA CACAGACGCG
    GAGCTGCCGG CCCAGGAGCC GGGCCTGCAG GGCCCCGGGG GTGCGGACCG CCGCCCGGAG
    ATGCGAGACC CCGAAGAGCT GTCCCCAGCC CTGGTGGTGT CCAGCTCACA AAGCTTTGTC
    ATCAGCGGCG GAGGCAGCAC GGTCACGGAA AACATACTGC ATTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965617.1
    CDS755..3982
    Translation

    Target ORF information:

    RefSeq Version XM_014110142.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris calcium sensing receptor (CASR), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110142.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATTTC ACAGCTGCTC TTTGATCCTC TTGGCAATCA CCTGGTGCAC TTCTGCCTAT 
    GGGCCTGACC AACGAGCCCA GAAGAAAGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
    CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTAAAGTCAA GGCCGGAGTC TGTGGAATGT
    ATCAGGTACA ATTTCCGCGG GTTTCGTTGG TTACAAGCAA TGATATTTGC CATCGAGGAA
    ATAAACAGCA GCCCAGCCCT TCTTCCAAAC ATGACACTGG GATACAGAAT ATTTGACACT
    TGCAACACCG TTTCTAAAGC CTTGGAGGCC ACTCTGAGTT TTGTGGCACA GAATAAAATT
    GATTCTCTGA ACCTTGACGA GTTCTGCAAC TGCTCAGAGC ATATCCCCTC TACTATCGCT
    GTGGTGGGAG CAACTGGCTC GGGCATCTCC ACGGCTGTGG CAAACCTGCT GGGCCTCTTC
    TACATCCCCC AGGTCAGCTA TGCCTCCTCC AGCAGACTCC TCAGCAATAA GAATCAGTTC
    AAGTCCTTCC TCCGTACCAT CCCCAATGAT GAACACCAGG CCACTGCCAT GGCAGACATT
    ATTGAGTATT TCCGCTGGAA CTGGGTGGGC ACCATTGCAG CTGATGATGA CTACGGCCGG
    CCAGGGATTG AGAAGTTCCG AGAGGAAGCA GAGGAGAGGG ACATCTGCAT CGACTTCAGT
    GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATTCAGC AAGTGGTAGA GGTGATCCAG
    AATTCCACAG CCAAAGTCAT TGTTGTTTTC TCCAGTGGCC CAGACCTTGA ACCCCTCATC
    AAGGAGATCG TCCGGCGAAA TATCACAGGA AGGATTTGGC TGGCCAGTGA GGCCTGGGCC
    AGCTCTTCCT TGATTGCCAT GCCCGAGTAC TTCCATGTGG TTGGAGGTAC CATTGGATTC
    GCTTTGAAGG CTGGGCAGAT CCCAGGTTTC CGGGAATTCC TGCAGAAAGT CCATCCCAGA
    AAGTCTGTCC ACAACGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
    CAAGAAGGTG CTAAAGGGCC TTTATCCATG GACACTTTCC TGAGAGGCCA CGAAGAAGGT
    GGTGGCAGGA TAAGCAACAG CTCCACTGCC TTCCGACCTC TTTGCACAGG AGATGAGAAC
    ATCAGTAGTG TGGAGACCCC TTATATGGAT TATACACACT TACGGATATC CTACAACGTC
    TACTTAGCAG TCTATTCCAT TGCTCATGCC CTGCAAGATA TATATACATG CTTACCTGGG
    AGAGGGCTCT TCACCAACGG TTCCTGTGCT GATATTAAGA AGGTTGAGGC TTGGCAGGTC
    TTGAAGCACC TACGGCACCT AAACTTTACC AACAATATGG GGGAGCAAGT GACTTTCGAT
    GAATGTGGTG ACCTGATGGG GAACTATTCC ATCATCAACT GGCACCTCTC TCCAGAGGAT
    GGCTCCATAG TGTTTAAGGA AGTCGGATAT TACAATGTCT ATGCCAAGAA AGGAGAAAGA
    CTCTTCATCA ATGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGAT GCCATTTTCC
    AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGAA TCATTGAGGG GGAGCCTACC
    TGCTGCTTTG AGTGTGTGGA GTGCCCCGAC GGGGAGTACA GTGATGAAAC AGATGCAAGT
    GCCTGTGACA AGTGCCCCGA TGACTTCTGG TCCAATGAAA ACCACACTTC GTGCATTGCC
    AAAGAGATTG AGTTTCTGTC CTGGACAGAG CCCTTTGGGA TTGCACTCAC CCTCTTTGCT
    GTGCTGGGCA TTTTCCTGAC AGCTTTCGTG CTGGGGGTCT TCATCAAGTT CCGTAACACG
    CCCATCGTCA AGGCCACCAA CCGAGAGCTC TCGTACCTCC TCCTCTTCTC CTTGCTGTGC
    TGCTTCTCCA GCTCCCTGTT CTTCATTGGC GAGCCCCAGG ACTGGACCTG CCGCCTGCGC
    CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATATCAT GCATCCTGGT GAAAACCAAC
    CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACAAGCT TCCACCGCAA GTGGTGGGGG
    CTCAACCTGC AGTTCCTGCT GGTCTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
    ATCTGGCTCT ACACGGCGCC TCCCTCCAGC TACCGCAACC ATGAGCTGGA GGACGAGATC
    ATCTTCATCA CATGCCACGA GGGCTCCCTG ATGGCCCTGG GCTTCCTGAT TGGCTACACC
    TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
    TTCAACGAGG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
    TTCATTCCAG CCTACGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTGGA AGTGATCGCC
    ATCCTGGCCG CCAGCTTTGG CCTCCTGGCC TGCATCTTCT TCAACAAGGT GTACATCATC
    CTCTTCAAGC CGTCCCGCAA CACCATCGAG GAGGTGCGCT GCAGCACCGC GGCTCACGCT
    TTCAAGGTCG CGGCCCGCGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCGGC
    AGCCTGGGGG GCTCCACGGG CTCCACGCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGT
    GAAGACCCCT TCCCGCAGCC CGAGAGGCAG AAGCAGCAGC AGCCCCTGGC CCTGACCCAG
    CGGGAGCAGC AGCCGCCGCA GCCCTTGACC TTGCCGCCGC AGCCGCAGCC CAGGTGCAAG
    CAGAAGGTCA TCTTCGGCAG TGGCACCGTC ACCTTCTCGC TGAGCTTTGA CGAGCCGCAG
    AAGAGCGCCG CGGCCCCCCG CAATTCCACG CTGCAGCACT CCCTGGAGGC CCAGCGGAGC
    CCCGAGCCCC CCGCCAGACC CCAGGCGTTA CTGCCGCCGC AGGGCGGAGA CACAGACGCG
    GAGCTGCCGG CCCAGGAGCC GGGCCTGCAG GGCCCCGGGG GTGCGGACCG CCGCCCGGAG
    ATGCGAGACC CCGAAGAGCT GTCCCCAGCC CTGGTGGTGT CCAGCTCACA AAGCTTTGTC
    ATCAGCGGCG GAGGCAGCAC GGTCACGGAA AACATACTGC ATTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.