USP53 cDNA ORF clone, Canis lupus familiaris(dog)

The following USP53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe284174 XM_022413702.1
Latest version!
Canis lupus familiaris ubiquitin specific peptidase 53 (USP53), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe284175 XM_022413703.1
Latest version!
Canis lupus familiaris ubiquitin specific peptidase 53 (USP53), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe29931 XM_005639323.3
Latest version!
Canis lupus familiaris ubiquitin specific peptidase 53 (USP53), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe29931 XM_005639324.2
Latest version!
Canis lupus familiaris ubiquitin specific peptidase 53 (USP53), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe29931 XM_022413701.1
Latest version!
Canis lupus familiaris ubiquitin specific peptidase 53 (USP53), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OCe29931 XM_005639323.2 Canis lupus familiaris ubiquitin specific peptidase 53 (USP53), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe284174
    Clone ID Related Accession (Same CDS sequence) XM_022413702.1
    Accession Version XM_022413702.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2991bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGATGATG CTGCAGAATG CTTTGAAAAT ATATTGGAGA GGATTCATTT TCATATAGTG 
    CCAAGCAGAG ATGCAGACAT GTGTACCTCT AAATCTTGTA TCACTCACCA GAAGTTTGCT
    ATGACACTGT ATGAACAGTG TGTGTGTCGT AGCTGTGGAG CATCATCAGA TCCTCTACCT
    TTTACAGAAT TTGTGCGGTA CATTTCTACA ACAGCCCTAT GCAATGAAGT AGAAAGAATG
    ATGGAAAGGC ACGAACGTTT TAAACCTGAA ATGTTTGCAG AATTGCTGCA AGCAGCAAAT
    ACAACTGATG ACTATAGGAA ATGTCCTAGT AACTGTGGCC AAAAAATAAA AATTCGCCGT
    GTTTTAATGA ATTGCCCAGA GATTGTTACA ATCGGTTTAG TATGGGATTC TGAGCATTCT
    GACTTGACAG AAGATGTTGT TCGGAATCTG GCAACACATC TTTATCTTCC TGGGCTTTTT
    TATAGAGTTA CTGATGAAAA TGCCAAAAAT AGTGAACTTC ACCTAGTTGG TATGATCTGC
    TACACCAGCC GACATTATTG TGCCTTTGCC TTTCATACCA AGAGTTCCAA ATGGGTATTT
    TTTGATGATG CAAATGTGAA AGAGGTTGGA ACTAGATGGA AGGATGTGGT CTCCAAATGT
    ATCCGATGCC ATTTCCAGCC ACTGCTTTTG TTTTACGCAA ACCCAGATGG CACAGCAGTT
    TCTACTGAAG ATGCGCTTAG GCAGGTCATC AACTGGTCAC ATTATAAATC CGTTGCAGAA
    AACATGGGAT GTGAAAAGCC CTCAATTTAT AAGTCAGATC ATTCAAAAGA AAATGGATTT
    TGTGATCAGG CGAAACAGAG AGAAAATCAG AAATTTCAAA CAGATAATAT TTCAGCATTT
    AGTCGGAGCC ATATTCAAAC AAGTGGTGGT GGAGGACCAG TTAAGTTAAC TCACAATGAT
    CAAAGGGAAA AAATAAAAGA CATTTCCAGA GAATGTGCTT TAAAAGCCAT TGAACAGAGG
    AACTTCCTTT CATCACAAAG GAAAGATTTG GAGAGGGGAC AAAGAAAAGA TTTGGGCCGA
    CATAGAGATT TGGTTGATGA AGACCTTCTG CATTTCAAGT CTGGATCACC TCCTGCCCCA
    AATGGCTTTA GACAATATGG AAATCCAAAT CTATATCATA GTCAAGGAAA AGGACCATGT
    AAACATGACC GAGTTGCCCA TCAGAGCCGA GCTTCTGCAC AAGTACTGAG TTCAAGTAAA
    TCCCAAATTC TTGCCCCAGG AGAGAAAGTA ACTGGCAAAG TTAAGAGTGA CAGTGGCACT
    GGATATGATA CAGATAGCAG CCAAGATTCT AGGGATAAAG GAAGCACCTA TAGTGGCAGC
    AGTAAAAGCC GGACCCGAGG TTGGAAACCT ATGAGAGAAA CACTAAATGT TGATAGTATT
    TTTAGTGAAA GTGAAAAAAG GCAGCATAGT CCAAGACATA AATCAAATAT CAGTAACAAA
    GCTAAATGTA GCAAAGATCA GAGTTTTAAC AATTGGTCAA AAGAGAACCC AAAGCAAAAA
    TGTTTAATGA CTATCTATGA AGATGAAATG AAGCAGGAAA TAGGAAGCAG GAGTTCCCTT
    GAATCTAATG GAAAAGGAGC AGAGAAAAAT AAAGGCCTCA AAGAGACTAC AGTTCATGGT
    GACAATTGGC AGATGCAAAG GACTGAGTCT GGATATGAAA GTAGTGATCA TGTCAGCAAT
    GGATCTGCTA ATTTGGATTC ACCTGTTGTC GATGGAAATG GTACAGGAAT GGATATCAGT
    GGTGTTAAAG AAACAGTATC CTGCAGTGAC CATGTTAGGA CAAGCAACAT AAATGTAGAG
    TATGTCAACT GTTCCTCCCA ACAGAGCAAA AACTACTTAG AAGCCTTAGG CTTTAGAAAA
    GAACTCAAGA ATTTGGAAGT AGGCTATAAA TCTCATGAGT TCCATCCAGA GTCACATCTA
    CAAATGAAAA ATCCTTTGAT AAAAAGATCA CATATACGTG AAATCAATGG AAAGTTATTC
    CCTTCATCAG GTCCACAGTT ACTCAAGGAC AATGCAGCAA GAGAGCATAT CCACCAGTCA
    GGTGAACAGA AACTTGAAAC CTCAAGTGAA TACAAATTTT CTGAGTGGCT TAATACAGAA
    AATTCTGAGA GAACAGGTTT ACTTTTTCAC AATGACAATT CTGCTTCTGG AAAAAGAATG
    AACAGTAATG AAATAGTCTC ACCATCATTG TGGTCTTCAC ACATGAGACC TGTTGGGTTA
    AAGCCAGAAA CTGCTCCCCT CATCCAGCAG CAAACTACGA TGGAACAATG TTGCTCTGAG
    AACTCTCTAT CCAGGGAACA TACAGTTCAG TCAACCTGCA GACCTGATGG TTGTCAGATG
    CCAAAACTTG TTAGCCATAA TCCACCGCCT CCTTTGCCAC CAAAGAAATA TGCGATAACC
    GGTGTGCCAC AGTCAGAGAA AAATGAATCC ACACCTAATG TCAAGCTTAC AGAGATGTTT
    AAAGCTAATT CTTCTAGTCT TCCAAAGCAC AGTTTAAGTC TGGATTCAGA ACCAGGTTCA
    GAAGAGGGTA CATATATGAA TGATGAAAGA CTTAGGGAAG CATTTCAAGT AAAAGAGCCT
    GTTGGCAACA CACCAAACTA CCTGTCCAGC TTTTCAACTG AAGATTTTCA GGCAGACTTG
    GCTACGGTTG TTGATGCATT TTGCCAACCA GAAATAGACT CTAGTTCTAC CTGTCCAAAT
    GAGACCATTT CATTAACTAC CTACTTCTCA GTTGATAGCT GCATGACAGA TACATACAGA
    CTGAAATACC ACCAGAGACC CAAGCTCTGT GTTCCAGAGA ACAGTGGCTT TTGTAATGAT
    GATGTACTAT CTAAGAGTGA AAGAAGGATA GCACCTGTGT TACACAACAG TCTTGGTTCA
    AGCTGTCCTT CTGAGCAGAG ATATAAACCT AGTATACATT GTAATAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269410.1
    CDS224..3214
    Translation

    Target ORF information:

    RefSeq Version XM_022413702.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ubiquitin specific peptidase 53 (USP53), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413702.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGATGATG CTGCAGAATG CTTTGAAAAT ATATTGGAGA GGATTCATTT TCATATAGTG 
    CCAAGCAGAG ATGCAGACAT GTGTACCTCT AAATCTTGTA TCACTCACCA GAAGTTTGCT
    ATGACACTGT ATGAACAGTG TGTGTGTCGT AGCTGTGGAG CATCATCAGA TCCTCTACCT
    TTTACAGAAT TTGTGCGGTA CATTTCTACA ACAGCCCTAT GCAATGAAGT AGAAAGAATG
    ATGGAAAGGC ACGAACGTTT TAAACCTGAA ATGTTTGCAG AATTGCTGCA AGCAGCAAAT
    ACAACTGATG ACTATAGGAA ATGTCCTAGT AACTGTGGCC AAAAAATAAA AATTCGCCGT
    GTTTTAATGA ATTGCCCAGA GATTGTTACA ATCGGTTTAG TATGGGATTC TGAGCATTCT
    GACTTGACAG AAGATGTTGT TCGGAATCTG GCAACACATC TTTATCTTCC TGGGCTTTTT
    TATAGAGTTA CTGATGAAAA TGCCAAAAAT AGTGAACTTC ACCTAGTTGG TATGATCTGC
    TACACCAGCC GACATTATTG TGCCTTTGCC TTTCATACCA AGAGTTCCAA ATGGGTATTT
    TTTGATGATG CAAATGTGAA AGAGGTTGGA ACTAGATGGA AGGATGTGGT CTCCAAATGT
    ATCCGATGCC ATTTCCAGCC ACTGCTTTTG TTTTACGCAA ACCCAGATGG CACAGCAGTT
    TCTACTGAAG ATGCGCTTAG GCAGGTCATC AACTGGTCAC ATTATAAATC CGTTGCAGAA
    AACATGGGAT GTGAAAAGCC CTCAATTTAT AAGTCAGATC ATTCAAAAGA AAATGGATTT
    TGTGATCAGG CGAAACAGAG AGAAAATCAG AAATTTCAAA CAGATAATAT TTCAGCATTT
    AGTCGGAGCC ATATTCAAAC AAGTGGTGGT GGAGGACCAG TTAAGTTAAC TCACAATGAT
    CAAAGGGAAA AAATAAAAGA CATTTCCAGA GAATGTGCTT TAAAAGCCAT TGAACAGAGG
    AACTTCCTTT CATCACAAAG GAAAGATTTG GAGAGGGGAC AAAGAAAAGA TTTGGGCCGA
    CATAGAGATT TGGTTGATGA AGACCTTCTG CATTTCAAGT CTGGATCACC TCCTGCCCCA
    AATGGCTTTA GACAATATGG AAATCCAAAT CTATATCATA GTCAAGGAAA AGGACCATGT
    AAACATGACC GAGTTGCCCA TCAGAGCCGA GCTTCTGCAC AAGTACTGAG TTCAAGTAAA
    TCCCAAATTC TTGCCCCAGG AGAGAAAGTA ACTGGCAAAG TTAAGAGTGA CAGTGGCACT
    GGATATGATA CAGATAGCAG CCAAGATTCT AGGGATAAAG GAAGCACCTA TAGTGGCAGC
    AGTAAAAGCC GGACCCGAGG TTGGAAACCT ATGAGAGAAA CACTAAATGT TGATAGTATT
    TTTAGTGAAA GTGAAAAAAG GCAGCATAGT CCAAGACATA AATCAAATAT CAGTAACAAA
    GCTAAATGTA GCAAAGATCA GAGTTTTAAC AATTGGTCAA AAGAGAACCC AAAGCAAAAA
    TGTTTAATGA CTATCTATGA AGATGAAATG AAGCAGGAAA TAGGAAGCAG GAGTTCCCTT
    GAATCTAATG GAAAAGGAGC AGAGAAAAAT AAAGGCCTCA AAGAGACTAC AGTTCATGGT
    GACAATTGGC AGATGCAAAG GACTGAGTCT GGATATGAAA GTAGTGATCA TGTCAGCAAT
    GGATCTGCTA ATTTGGATTC ACCTGTTGTC GATGGAAATG GTACAGGAAT GGATATCAGT
    GGTGTTAAAG AAACAGTATC CTGCAGTGAC CATGTTAGGA CAAGCAACAT AAATGTAGAG
    TATGTCAACT GTTCCTCCCA ACAGAGCAAA AACTACTTAG AAGCCTTAGG CTTTAGAAAA
    GAACTCAAGA ATTTGGAAGT AGGCTATAAA TCTCATGAGT TCCATCCAGA GTCACATCTA
    CAAATGAAAA ATCCTTTGAT AAAAAGATCA CATATACGTG AAATCAATGG AAAGTTATTC
    CCTTCATCAG GTCCACAGTT ACTCAAGGAC AATGCAGCAA GAGAGCATAT CCACCAGTCA
    GGTGAACAGA AACTTGAAAC CTCAAGTGAA TACAAATTTT CTGAGTGGCT TAATACAGAA
    AATTCTGAGA GAACAGGTTT ACTTTTTCAC AATGACAATT CTGCTTCTGG AAAAAGAATG
    AACAGTAATG AAATAGTCTC ACCATCATTG TGGTCTTCAC ACATGAGACC TGTTGGGTTA
    AAGCCAGAAA CTGCTCCCCT CATCCAGCAG CAAACTACGA TGGAACAATG TTGCTCTGAG
    AACTCTCTAT CCAGGGAACA TACAGTTCAG TCAACCTGCA GACCTGATGG TTGTCAGATG
    CCAAAACTTG TTAGCCATAA TCCACCGCCT CCTTTGCCAC CAAAGAAATA TGCGATAACC
    GGTGTGCCAC AGTCAGAGAA AAATGAATCC ACACCTAATG TCAAGCTTAC AGAGATGTTT
    AAAGCTAATT CTTCTAGTCT TCCAAAGCAC AGTTTAAGTC TGGATTCAGA ACCAGGTTCA
    GAAGAGGGTA CATATATGAA TGATGAAAGA CTTAGGGAAG CATTTCAAGT AAAAGAGCCT
    GTTGGCAACA CACCAAACTA CCTGTCCAGC TTTTCAACTG AAGATTTTCA GGCAGACTTG
    GCTACGGTTG TTGATGCATT TTGCCAACCA GAAATAGACT CTAGTTCTAC CTGTCCAAAT
    GAGACCATTT CATTAACTAC CTACTTCTCA GTTGATAGCT GCATGACAGA TACATACAGA
    CTGAAATACC ACCAGAGACC CAAGCTCTGT GTTCCAGAGA ACAGTGGCTT TTGTAATGAT
    GATGTACTAT CTAAGAGTGA AAGAAGGATA GCACCTGTGT TACACAACAG TCTTGGTTCA
    AGCTGTCCTT CTGAGCAGAG ATATAAACCT AGTATACATT GTAATAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284175
    Clone ID Related Accession (Same CDS sequence) XM_022413703.1
    Accession Version XM_022413703.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2754bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGATGGAAA GGCACGAACG TTTTAAACCT GAAATGTTTG CAGAATTGCT GCAAGCAGCA 
    AATACAACTG ATGACTATAG GAAATGTCCT AGTAACTGTG GCCAAAAAAT AAAAATTCGC
    CGTGTTTTAA TGAATTGCCC AGAGATTGTT ACAATCGGTT TAGTATGGGA TTCTGAGCAT
    TCTGACTTGA CAGAAGATGT TGTTCGGAAT CTGGCAACAC ATCTTTATCT TCCTGGGCTT
    TTTTATAGAG TTACTGATGA AAATGCCAAA AATAGTGAAC TTCACCTAGT TGGTATGATC
    TGCTACACCA GCCGACATTA TTGTGCCTTT GCCTTTCATA CCAAGAGTTC CAAATGGGTA
    TTTTTTGATG ATGCAAATGT GAAAGAGGTT GGAACTAGAT GGAAGGATGT GGTCTCCAAA
    TGTATCCGAT GCCATTTCCA GCCACTGCTT TTGTTTTACG CAAACCCAGA TGGCACAGCA
    GTTTCTACTG AAGATGCGCT TAGGCAGGTC ATCAACTGGT CACATTATAA ATCCGTTGCA
    GAAAACATGG GATGTGAAAA GCCCTCAATT TATAAGTCAG ATCATTCAAA AGAAAATGGA
    TTTTGTGATC AGGCGAAACA GAGAGAAAAT CAGAAATTTC AAACAGATAA TATTTCAGCA
    TTTAGTCGGA GCCATATTCA AACAAGTGGT GGTGGAGGAC CAGTTAAGTT AACTCACAAT
    GATCAAAGGG AAAAAATAAA AGACATTTCC AGAGAATGTG CTTTAAAAGC CATTGAACAG
    AGGAACTTCC TTTCATCACA AAGGAAAGAT TTGGAGAGGG GACAAAGAAA AGATTTGGGC
    CGACATAGAG ATTTGGTTGA TGAAGACCTT CTGCATTTCA AGTCTGGATC ACCTCCTGCC
    CCAAATGGCT TTAGACAATA TGGAAATCCA AATCTATATC ATAGTCAAGG AAAAGGACCA
    TGTAAACATG ACCGAGTTGC CCATCAGAGC CGAGCTTCTG CACAAGTACT GAGTTCAAGT
    AAATCCCAAA TTCTTGCCCC AGGAGAGAAA GTAACTGGCA AAGTTAAGAG TGACAGTGGC
    ACTGGATATG ATACAGATAG CAGCCAAGAT TCTAGGGATA AAGGAAGCAC CTATAGTGGC
    AGCAGTAAAA GCCGGACCCG AGGTTGGAAA CCTATGAGAG AAACACTAAA TGTTGATAGT
    ATTTTTAGTG AAAGTGAAAA AAGGCAGCAT AGTCCAAGAC ATAAATCAAA TATCAGTAAC
    AAAGCTAAAT GTAGCAAAGA TCAGAGTTTT AACAATTGGT CAAAAGAGAA CCCAAAGCAA
    AAATGTTTAA TGACTATCTA TGAAGATGAA ATGAAGCAGG AAATAGGAAG CAGGAGTTCC
    CTTGAATCTA ATGGAAAAGG AGCAGAGAAA AATAAAGGCC TCAAAGAGAC TACAGTTCAT
    GGTGACAATT GGCAGATGCA AAGGACTGAG TCTGGATATG AAAGTAGTGA TCATGTCAGC
    AATGGATCTG CTAATTTGGA TTCACCTGTT GTCGATGGAA ATGGTACAGG AATGGATATC
    AGTGGTGTTA AAGAAACAGT ATCCTGCAGT GACCATGTTA GGACAAGCAA CATAAATGTA
    GAGTATGTCA ACTGTTCCTC CCAACAGAGC AAAAACTACT TAGAAGCCTT AGGCTTTAGA
    AAAGAACTCA AGAATTTGGA AGTAGGCTAT AAATCTCATG AGTTCCATCC AGAGTCACAT
    CTACAAATGA AAAATCCTTT GATAAAAAGA TCACATATAC GTGAAATCAA TGGAAAGTTA
    TTCCCTTCAT CAGGTCCACA GTTACTCAAG GACAATGCAG CAAGAGAGCA TATCCACCAG
    TCAGGTGAAC AGAAACTTGA AACCTCAAGT GAATACAAAT TTTCTGAGTG GCTTAATACA
    GAAAATTCTG AGAGAACAGG TTTACTTTTT CACAATGACA ATTCTGCTTC TGGAAAAAGA
    ATGAACAGTA ATGAAATAGT CTCACCATCA TTGTGGTCTT CACACATGAG ACCTGTTGGG
    TTAAAGCCAG AAACTGCTCC CCTCATCCAG CAGCAAACTA CGATGGAACA ATGTTGCTCT
    GAGAACTCTC TATCCAGGGA ACATACAGTT CAGTCAACCT GCAGACCTGA TGGTTGTCAG
    ATGCCAAAAC TTGTTAGCCA TAATCCACCG CCTCCTTTGC CACCAAAGAA ATATGCGATA
    ACCGGTGTGC CACAGTCAGA GAAAAATGAA TCCACACCTA ATGTCAAGCT TACAGAGATG
    TTTAAAGCTA ATTCTTCTAG TCTTCCAAAG CACAGTTTAA GTCTGGATTC AGAACCAGGT
    TCAGAAGAGG GTACATATAT GAATGATGAA AGACTTAGGG AAGCATTTCA AGTAAAAGAG
    CCTGTTGGCA ACACACCAAA CTACCTGTCC AGCTTTTCAA CTGAAGATTT TCAGGCAGAC
    TTGGCTACGG TTGTTGATGC ATTTTGCCAA CCAGAAATAG ACTCTAGTTC TACCTGTCCA
    AATGAGACCA TTTCATTAAC TACCTACTTC TCAGTTGATA GCTGCATGAC AGATACATAC
    AGACTGAAAT ACCACCAGAG ACCCAAGCTC TGTGTTCCAG AGAACAGTGG CTTTTGTAAT
    GATGATGTAC TATCTAAGAG TGAAAGAAGG ATAGCACCTG TGTTACACAA CAGTCTTGGT
    TCAAGCTGTC CTTCTGAGCA GAGATATAAA CCTAGTATAC ATTGTAATAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269411.1
    CDS129..2882
    Translation

    Target ORF information:

    RefSeq Version XM_022413703.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ubiquitin specific peptidase 53 (USP53), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413703.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGATGGAAA GGCACGAACG TTTTAAACCT GAAATGTTTG CAGAATTGCT GCAAGCAGCA 
    AATACAACTG ATGACTATAG GAAATGTCCT AGTAACTGTG GCCAAAAAAT AAAAATTCGC
    CGTGTTTTAA TGAATTGCCC AGAGATTGTT ACAATCGGTT TAGTATGGGA TTCTGAGCAT
    TCTGACTTGA CAGAAGATGT TGTTCGGAAT CTGGCAACAC ATCTTTATCT TCCTGGGCTT
    TTTTATAGAG TTACTGATGA AAATGCCAAA AATAGTGAAC TTCACCTAGT TGGTATGATC
    TGCTACACCA GCCGACATTA TTGTGCCTTT GCCTTTCATA CCAAGAGTTC CAAATGGGTA
    TTTTTTGATG ATGCAAATGT GAAAGAGGTT GGAACTAGAT GGAAGGATGT GGTCTCCAAA
    TGTATCCGAT GCCATTTCCA GCCACTGCTT TTGTTTTACG CAAACCCAGA TGGCACAGCA
    GTTTCTACTG AAGATGCGCT TAGGCAGGTC ATCAACTGGT CACATTATAA ATCCGTTGCA
    GAAAACATGG GATGTGAAAA GCCCTCAATT TATAAGTCAG ATCATTCAAA AGAAAATGGA
    TTTTGTGATC AGGCGAAACA GAGAGAAAAT CAGAAATTTC AAACAGATAA TATTTCAGCA
    TTTAGTCGGA GCCATATTCA AACAAGTGGT GGTGGAGGAC CAGTTAAGTT AACTCACAAT
    GATCAAAGGG AAAAAATAAA AGACATTTCC AGAGAATGTG CTTTAAAAGC CATTGAACAG
    AGGAACTTCC TTTCATCACA AAGGAAAGAT TTGGAGAGGG GACAAAGAAA AGATTTGGGC
    CGACATAGAG ATTTGGTTGA TGAAGACCTT CTGCATTTCA AGTCTGGATC ACCTCCTGCC
    CCAAATGGCT TTAGACAATA TGGAAATCCA AATCTATATC ATAGTCAAGG AAAAGGACCA
    TGTAAACATG ACCGAGTTGC CCATCAGAGC CGAGCTTCTG CACAAGTACT GAGTTCAAGT
    AAATCCCAAA TTCTTGCCCC AGGAGAGAAA GTAACTGGCA AAGTTAAGAG TGACAGTGGC
    ACTGGATATG ATACAGATAG CAGCCAAGAT TCTAGGGATA AAGGAAGCAC CTATAGTGGC
    AGCAGTAAAA GCCGGACCCG AGGTTGGAAA CCTATGAGAG AAACACTAAA TGTTGATAGT
    ATTTTTAGTG AAAGTGAAAA AAGGCAGCAT AGTCCAAGAC ATAAATCAAA TATCAGTAAC
    AAAGCTAAAT GTAGCAAAGA TCAGAGTTTT AACAATTGGT CAAAAGAGAA CCCAAAGCAA
    AAATGTTTAA TGACTATCTA TGAAGATGAA ATGAAGCAGG AAATAGGAAG CAGGAGTTCC
    CTTGAATCTA ATGGAAAAGG AGCAGAGAAA AATAAAGGCC TCAAAGAGAC TACAGTTCAT
    GGTGACAATT GGCAGATGCA AAGGACTGAG TCTGGATATG AAAGTAGTGA TCATGTCAGC
    AATGGATCTG CTAATTTGGA TTCACCTGTT GTCGATGGAA ATGGTACAGG AATGGATATC
    AGTGGTGTTA AAGAAACAGT ATCCTGCAGT GACCATGTTA GGACAAGCAA CATAAATGTA
    GAGTATGTCA ACTGTTCCTC CCAACAGAGC AAAAACTACT TAGAAGCCTT AGGCTTTAGA
    AAAGAACTCA AGAATTTGGA AGTAGGCTAT AAATCTCATG AGTTCCATCC AGAGTCACAT
    CTACAAATGA AAAATCCTTT GATAAAAAGA TCACATATAC GTGAAATCAA TGGAAAGTTA
    TTCCCTTCAT CAGGTCCACA GTTACTCAAG GACAATGCAG CAAGAGAGCA TATCCACCAG
    TCAGGTGAAC AGAAACTTGA AACCTCAAGT GAATACAAAT TTTCTGAGTG GCTTAATACA
    GAAAATTCTG AGAGAACAGG TTTACTTTTT CACAATGACA ATTCTGCTTC TGGAAAAAGA
    ATGAACAGTA ATGAAATAGT CTCACCATCA TTGTGGTCTT CACACATGAG ACCTGTTGGG
    TTAAAGCCAG AAACTGCTCC CCTCATCCAG CAGCAAACTA CGATGGAACA ATGTTGCTCT
    GAGAACTCTC TATCCAGGGA ACATACAGTT CAGTCAACCT GCAGACCTGA TGGTTGTCAG
    ATGCCAAAAC TTGTTAGCCA TAATCCACCG CCTCCTTTGC CACCAAAGAA ATATGCGATA
    ACCGGTGTGC CACAGTCAGA GAAAAATGAA TCCACACCTA ATGTCAAGCT TACAGAGATG
    TTTAAAGCTA ATTCTTCTAG TCTTCCAAAG CACAGTTTAA GTCTGGATTC AGAACCAGGT
    TCAGAAGAGG GTACATATAT GAATGATGAA AGACTTAGGG AAGCATTTCA AGTAAAAGAG
    CCTGTTGGCA ACACACCAAA CTACCTGTCC AGCTTTTCAA CTGAAGATTT TCAGGCAGAC
    TTGGCTACGG TTGTTGATGC ATTTTGCCAA CCAGAAATAG ACTCTAGTTC TACCTGTCCA
    AATGAGACCA TTTCATTAAC TACCTACTTC TCAGTTGATA GCTGCATGAC AGATACATAC
    AGACTGAAAT ACCACCAGAG ACCCAAGCTC TGTGTTCCAG AGAACAGTGG CTTTTGTAAT
    GATGATGTAC TATCTAAGAG TGAAAGAAGG ATAGCACCTG TGTTACACAA CAGTCTTGGT
    TCAAGCTGTC CTTCTGAGCA GAGATATAAA CCTAGTATAC ATTGTAATAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29931
    Clone ID Related Accession (Same CDS sequence) XM_005639323.2 , XM_005639324.2 , XM_005639323.3 , XM_022413701.1
    Accession Version XM_005639323.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3339bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726116.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005639323.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCATGGG TAAAATTCTT ACGGAAACCT AGTGGCAATC TTGGAAAAGT TTATCAACCT 
    GGAAGTATGC TATCCCTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGTGCTGT ACAGGTTTTA TGGCAATTGG ATATATTTCG AAGAAGCTTG
    CGGGTTCTAA CTGGGCATGT TTGTCAGGGA GATGCCTGCA TATTTTGTGC ATTAAAGACA
    ATATTTGCAC AGTTTCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAC
    GCTCTAGCGG AAAGCTTCAA AGATGAGCAG CGATTTCAGC TGGGTCTTAT GGATGATGCT
    GCAGAATGCT TTGAAAATAT ATTGGAGAGG ATTCATTTTC ATATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACACTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCATCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCCTATGC AATGAAGTAG AAAGAATGAT GGAAAGGCAC
    GAACGTTTTA AACCTGAAAT GTTTGCAGAA TTGCTGCAAG CAGCAAATAC AACTGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT CGGTTTAGTA TGGGATTCTG AGCATTCTGA CTTGACAGAA
    GATGTTGTTC GGAATCTGGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTCAC CTAGTTGGTA TGATCTGCTA CACCAGCCGA
    CATTATTGTG CCTTTGCCTT TCATACCAAG AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGGTTGGAAC TAGATGGAAG GATGTGGTCT CCAAATGTAT CCGATGCCAT
    TTCCAGCCAC TGCTTTTGTT TTACGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAAGAT
    GCGCTTAGGC AGGTCATCAA CTGGTCACAT TATAAATCCG TTGCAGAAAA CATGGGATGT
    GAAAAGCCCT CAATTTATAA GTCAGATCAT TCAAAAGAAA ATGGATTTTG TGATCAGGCG
    AAACAGAGAG AAAATCAGAA ATTTCAAACA GATAATATTT CAGCATTTAG TCGGAGCCAT
    ATTCAAACAA GTGGTGGTGG AGGACCAGTT AAGTTAACTC ACAATGATCA AAGGGAAAAA
    ATAAAAGACA TTTCCAGAGA ATGTGCTTTA AAAGCCATTG AACAGAGGAA CTTCCTTTCA
    TCACAAAGGA AAGATTTGGA GAGGGGACAA AGAAAAGATT TGGGCCGACA TAGAGATTTG
    GTTGATGAAG ACCTTCTGCA TTTCAAGTCT GGATCACCTC CTGCCCCAAA TGGCTTTAGA
    CAATATGGAA ATCCAAATCT ATATCATAGT CAAGGAAAAG GACCATGTAA ACATGACCGA
    GTTGCCCATC AGAGCCGAGC TTCTGCACAA GTACTGAGTT CAAGTAAATC CCAAATTCTT
    GCCCCAGGAG AGAAAGTAAC TGGCAAAGTT AAGAGTGACA GTGGCACTGG ATATGATACA
    GATAGCAGCC AAGATTCTAG GGATAAAGGA AGCACCTATA GTGGCAGCAG TAAAAGCCGG
    ACCCGAGGTT GGAAACCTAT GAGAGAAACA CTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGGC AGCATAGTCC AAGACATAAA TCAAATATCA GTAACAAAGC TAAATGTAGC
    AAAGATCAGA GTTTTAACAA TTGGTCAAAA GAGAACCCAA AGCAAAAATG TTTAATGACT
    ATCTATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGGA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTCAAA GAGACTACAG TTCATGGTGA CAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGT AGTGATCATG TCAGCAATGG ATCTGCTAAT
    TTGGATTCAC CTGTTGTCGA TGGAAATGGT ACAGGAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATCCT GCAGTGACCA TGTTAGGACA AGCAACATAA ATGTAGAGTA TGTCAACTGT
    TCCTCCCAAC AGAGCAAAAA CTACTTAGAA GCCTTAGGCT TTAGAAAAGA ACTCAAGAAT
    TTGGAAGTAG GCTATAAATC TCATGAGTTC CATCCAGAGT CACATCTACA AATGAAAAAT
    CCTTTGATAA AAAGATCACA TATACGTGAA ATCAATGGAA AGTTATTCCC TTCATCAGGT
    CCACAGTTAC TCAAGGACAA TGCAGCAAGA GAGCATATCC ACCAGTCAGG TGAACAGAAA
    CTTGAAACCT CAAGTGAATA CAAATTTTCT GAGTGGCTTA ATACAGAAAA TTCTGAGAGA
    ACAGGTTTAC TTTTTCACAA TGACAATTCT GCTTCTGGAA AAAGAATGAA CAGTAATGAA
    ATAGTCTCAC CATCATTGTG GTCTTCACAC ATGAGACCTG TTGGGTTAAA GCCAGAAACT
    GCTCCCCTCA TCCAGCAGCA AACTACGATG GAACAATGTT GCTCTGAGAA CTCTCTATCC
    AGGGAACATA CAGTTCAGTC AACCTGCAGA CCTGATGGTT GTCAGATGCC AAAACTTGTT
    AGCCATAATC CACCGCCTCC TTTGCCACCA AAGAAATATG CGATAACCGG TGTGCCACAG
    TCAGAGAAAA ATGAATCCAC ACCTAATGTC AAGCTTACAG AGATGTTTAA AGCTAATTCT
    TCTAGTCTTC CAAAGCACAG TTTAAGTCTG GATTCAGAAC CAGGTTCAGA AGAGGGTACA
    TATATGAATG ATGAAAGACT TAGGGAAGCA TTTCAAGTAA AAGAGCCTGT TGGCAACACA
    CCAAACTACC TGTCCAGCTT TTCAACTGAA GATTTTCAGG CAGACTTGGC TACGGTTGTT
    GATGCATTTT GCCAACCAGA AATAGACTCT AGTTCTACCT GTCCAAATGA GACCATTTCA
    TTAACTACCT ACTTCTCAGT TGATAGCTGC ATGACAGATA CATACAGACT GAAATACCAC
    CAGAGACCCA AGCTCTGTGT TCCAGAGAAC AGTGGCTTTT GTAATGATGA TGTACTATCT
    AAGAGTGAAA GAAGGATAGC ACCTGTGTTA CACAACAGTC TTGGTTCAAG CTGTCCTTCT
    GAGCAGAGAT ATAAACCTAG TATACATTGT AATAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005639380.1
    CDS804..4142
    Translation

    Target ORF information:

    RefSeq Version XM_005639323.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ubiquitin specific peptidase 53 (USP53), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005639323.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCATGGG TAAAATTCTT ACGGAAACCT AGTGGCAATC TTGGAAAAGT TTATCAACCT 
    GGAAGTATGC TATCCCTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGTGCTGT ACAGGTTTTA TGGCAATTGG ATATATTTCG AAGAAGCTTG
    CGGGTTCTAA CTGGGCATGT TTGTCAGGGA GATGCCTGCA TATTTTGTGC ATTAAAGACA
    ATATTTGCAC AGTTTCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAC
    GCTCTAGCGG AAAGCTTCAA AGATGAGCAG CGATTTCAGC TGGGTCTTAT GGATGATGCT
    GCAGAATGCT TTGAAAATAT ATTGGAGAGG ATTCATTTTC ATATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACACTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCATCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCCTATGC AATGAAGTAG AAAGAATGAT GGAAAGGCAC
    GAACGTTTTA AACCTGAAAT GTTTGCAGAA TTGCTGCAAG CAGCAAATAC AACTGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT CGGTTTAGTA TGGGATTCTG AGCATTCTGA CTTGACAGAA
    GATGTTGTTC GGAATCTGGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTCAC CTAGTTGGTA TGATCTGCTA CACCAGCCGA
    CATTATTGTG CCTTTGCCTT TCATACCAAG AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGGTTGGAAC TAGATGGAAG GATGTGGTCT CCAAATGTAT CCGATGCCAT
    TTCCAGCCAC TGCTTTTGTT TTACGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAAGAT
    GCGCTTAGGC AGGTCATCAA CTGGTCACAT TATAAATCCG TTGCAGAAAA CATGGGATGT
    GAAAAGCCCT CAATTTATAA GTCAGATCAT TCAAAAGAAA ATGGATTTTG TGATCAGGCG
    AAACAGAGAG AAAATCAGAA ATTTCAAACA GATAATATTT CAGCATTTAG TCGGAGCCAT
    ATTCAAACAA GTGGTGGTGG AGGACCAGTT AAGTTAACTC ACAATGATCA AAGGGAAAAA
    ATAAAAGACA TTTCCAGAGA ATGTGCTTTA AAAGCCATTG AACAGAGGAA CTTCCTTTCA
    TCACAAAGGA AAGATTTGGA GAGGGGACAA AGAAAAGATT TGGGCCGACA TAGAGATTTG
    GTTGATGAAG ACCTTCTGCA TTTCAAGTCT GGATCACCTC CTGCCCCAAA TGGCTTTAGA
    CAATATGGAA ATCCAAATCT ATATCATAGT CAAGGAAAAG GACCATGTAA ACATGACCGA
    GTTGCCCATC AGAGCCGAGC TTCTGCACAA GTACTGAGTT CAAGTAAATC CCAAATTCTT
    GCCCCAGGAG AGAAAGTAAC TGGCAAAGTT AAGAGTGACA GTGGCACTGG ATATGATACA
    GATAGCAGCC AAGATTCTAG GGATAAAGGA AGCACCTATA GTGGCAGCAG TAAAAGCCGG
    ACCCGAGGTT GGAAACCTAT GAGAGAAACA CTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGGC AGCATAGTCC AAGACATAAA TCAAATATCA GTAACAAAGC TAAATGTAGC
    AAAGATCAGA GTTTTAACAA TTGGTCAAAA GAGAACCCAA AGCAAAAATG TTTAATGACT
    ATCTATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGGA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTCAAA GAGACTACAG TTCATGGTGA CAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGT AGTGATCATG TCAGCAATGG ATCTGCTAAT
    TTGGATTCAC CTGTTGTCGA TGGAAATGGT ACAGGAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATCCT GCAGTGACCA TGTTAGGACA AGCAACATAA ATGTAGAGTA TGTCAACTGT
    TCCTCCCAAC AGAGCAAAAA CTACTTAGAA GCCTTAGGCT TTAGAAAAGA ACTCAAGAAT
    TTGGAAGTAG GCTATAAATC TCATGAGTTC CATCCAGAGT CACATCTACA AATGAAAAAT
    CCTTTGATAA AAAGATCACA TATACGTGAA ATCAATGGAA AGTTATTCCC TTCATCAGGT
    CCACAGTTAC TCAAGGACAA TGCAGCAAGA GAGCATATCC ACCAGTCAGG TGAACAGAAA
    CTTGAAACCT CAAGTGAATA CAAATTTTCT GAGTGGCTTA ATACAGAAAA TTCTGAGAGA
    ACAGGTTTAC TTTTTCACAA TGACAATTCT GCTTCTGGAA AAAGAATGAA CAGTAATGAA
    ATAGTCTCAC CATCATTGTG GTCTTCACAC ATGAGACCTG TTGGGTTAAA GCCAGAAACT
    GCTCCCCTCA TCCAGCAGCA AACTACGATG GAACAATGTT GCTCTGAGAA CTCTCTATCC
    AGGGAACATA CAGTTCAGTC AACCTGCAGA CCTGATGGTT GTCAGATGCC AAAACTTGTT
    AGCCATAATC CACCGCCTCC TTTGCCACCA AAGAAATATG CGATAACCGG TGTGCCACAG
    TCAGAGAAAA ATGAATCCAC ACCTAATGTC AAGCTTACAG AGATGTTTAA AGCTAATTCT
    TCTAGTCTTC CAAAGCACAG TTTAAGTCTG GATTCAGAAC CAGGTTCAGA AGAGGGTACA
    TATATGAATG ATGAAAGACT TAGGGAAGCA TTTCAAGTAA AAGAGCCTGT TGGCAACACA
    CCAAACTACC TGTCCAGCTT TTCAACTGAA GATTTTCAGG CAGACTTGGC TACGGTTGTT
    GATGCATTTT GCCAACCAGA AATAGACTCT AGTTCTACCT GTCCAAATGA GACCATTTCA
    TTAACTACCT ACTTCTCAGT TGATAGCTGC ATGACAGATA CATACAGACT GAAATACCAC
    CAGAGACCCA AGCTCTGTGT TCCAGAGAAC AGTGGCTTTT GTAATGATGA TGTACTATCT
    AAGAGTGAAA GAAGGATAGC ACCTGTGTTA CACAACAGTC TTGGTTCAAG CTGTCCTTCT
    GAGCAGAGAT ATAAACCTAG TATACATTGT AATAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29931
    Clone ID Related Accession (Same CDS sequence) XM_005639323.2 , XM_005639324.2 , XM_005639323.3 , XM_022413701.1
    Accession Version XM_005639324.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3339bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726116.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005639324.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCATGGG TAAAATTCTT ACGGAAACCT AGTGGCAATC TTGGAAAAGT TTATCAACCT 
    GGAAGTATGC TATCCCTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGTGCTGT ACAGGTTTTA TGGCAATTGG ATATATTTCG AAGAAGCTTG
    CGGGTTCTAA CTGGGCATGT TTGTCAGGGA GATGCCTGCA TATTTTGTGC ATTAAAGACA
    ATATTTGCAC AGTTTCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAC
    GCTCTAGCGG AAAGCTTCAA AGATGAGCAG CGATTTCAGC TGGGTCTTAT GGATGATGCT
    GCAGAATGCT TTGAAAATAT ATTGGAGAGG ATTCATTTTC ATATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACACTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCATCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCCTATGC AATGAAGTAG AAAGAATGAT GGAAAGGCAC
    GAACGTTTTA AACCTGAAAT GTTTGCAGAA TTGCTGCAAG CAGCAAATAC AACTGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT CGGTTTAGTA TGGGATTCTG AGCATTCTGA CTTGACAGAA
    GATGTTGTTC GGAATCTGGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTCAC CTAGTTGGTA TGATCTGCTA CACCAGCCGA
    CATTATTGTG CCTTTGCCTT TCATACCAAG AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGGTTGGAAC TAGATGGAAG GATGTGGTCT CCAAATGTAT CCGATGCCAT
    TTCCAGCCAC TGCTTTTGTT TTACGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAAGAT
    GCGCTTAGGC AGGTCATCAA CTGGTCACAT TATAAATCCG TTGCAGAAAA CATGGGATGT
    GAAAAGCCCT CAATTTATAA GTCAGATCAT TCAAAAGAAA ATGGATTTTG TGATCAGGCG
    AAACAGAGAG AAAATCAGAA ATTTCAAACA GATAATATTT CAGCATTTAG TCGGAGCCAT
    ATTCAAACAA GTGGTGGTGG AGGACCAGTT AAGTTAACTC ACAATGATCA AAGGGAAAAA
    ATAAAAGACA TTTCCAGAGA ATGTGCTTTA AAAGCCATTG AACAGAGGAA CTTCCTTTCA
    TCACAAAGGA AAGATTTGGA GAGGGGACAA AGAAAAGATT TGGGCCGACA TAGAGATTTG
    GTTGATGAAG ACCTTCTGCA TTTCAAGTCT GGATCACCTC CTGCCCCAAA TGGCTTTAGA
    CAATATGGAA ATCCAAATCT ATATCATAGT CAAGGAAAAG GACCATGTAA ACATGACCGA
    GTTGCCCATC AGAGCCGAGC TTCTGCACAA GTACTGAGTT CAAGTAAATC CCAAATTCTT
    GCCCCAGGAG AGAAAGTAAC TGGCAAAGTT AAGAGTGACA GTGGCACTGG ATATGATACA
    GATAGCAGCC AAGATTCTAG GGATAAAGGA AGCACCTATA GTGGCAGCAG TAAAAGCCGG
    ACCCGAGGTT GGAAACCTAT GAGAGAAACA CTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGGC AGCATAGTCC AAGACATAAA TCAAATATCA GTAACAAAGC TAAATGTAGC
    AAAGATCAGA GTTTTAACAA TTGGTCAAAA GAGAACCCAA AGCAAAAATG TTTAATGACT
    ATCTATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGGA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTCAAA GAGACTACAG TTCATGGTGA CAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGT AGTGATCATG TCAGCAATGG ATCTGCTAAT
    TTGGATTCAC CTGTTGTCGA TGGAAATGGT ACAGGAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATCCT GCAGTGACCA TGTTAGGACA AGCAACATAA ATGTAGAGTA TGTCAACTGT
    TCCTCCCAAC AGAGCAAAAA CTACTTAGAA GCCTTAGGCT TTAGAAAAGA ACTCAAGAAT
    TTGGAAGTAG GCTATAAATC TCATGAGTTC CATCCAGAGT CACATCTACA AATGAAAAAT
    CCTTTGATAA AAAGATCACA TATACGTGAA ATCAATGGAA AGTTATTCCC TTCATCAGGT
    CCACAGTTAC TCAAGGACAA TGCAGCAAGA GAGCATATCC ACCAGTCAGG TGAACAGAAA
    CTTGAAACCT CAAGTGAATA CAAATTTTCT GAGTGGCTTA ATACAGAAAA TTCTGAGAGA
    ACAGGTTTAC TTTTTCACAA TGACAATTCT GCTTCTGGAA AAAGAATGAA CAGTAATGAA
    ATAGTCTCAC CATCATTGTG GTCTTCACAC ATGAGACCTG TTGGGTTAAA GCCAGAAACT
    GCTCCCCTCA TCCAGCAGCA AACTACGATG GAACAATGTT GCTCTGAGAA CTCTCTATCC
    AGGGAACATA CAGTTCAGTC AACCTGCAGA CCTGATGGTT GTCAGATGCC AAAACTTGTT
    AGCCATAATC CACCGCCTCC TTTGCCACCA AAGAAATATG CGATAACCGG TGTGCCACAG
    TCAGAGAAAA ATGAATCCAC ACCTAATGTC AAGCTTACAG AGATGTTTAA AGCTAATTCT
    TCTAGTCTTC CAAAGCACAG TTTAAGTCTG GATTCAGAAC CAGGTTCAGA AGAGGGTACA
    TATATGAATG ATGAAAGACT TAGGGAAGCA TTTCAAGTAA AAGAGCCTGT TGGCAACACA
    CCAAACTACC TGTCCAGCTT TTCAACTGAA GATTTTCAGG CAGACTTGGC TACGGTTGTT
    GATGCATTTT GCCAACCAGA AATAGACTCT AGTTCTACCT GTCCAAATGA GACCATTTCA
    TTAACTACCT ACTTCTCAGT TGATAGCTGC ATGACAGATA CATACAGACT GAAATACCAC
    CAGAGACCCA AGCTCTGTGT TCCAGAGAAC AGTGGCTTTT GTAATGATGA TGTACTATCT
    AAGAGTGAAA GAAGGATAGC ACCTGTGTTA CACAACAGTC TTGGTTCAAG CTGTCCTTCT
    GAGCAGAGAT ATAAACCTAG TATACATTGT AATAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005639381.1
    CDS1398..4736
    Translation

    Target ORF information:

    RefSeq Version XM_005639324.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ubiquitin specific peptidase 53 (USP53), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005639324.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCATGGG TAAAATTCTT ACGGAAACCT AGTGGCAATC TTGGAAAAGT TTATCAACCT 
    GGAAGTATGC TATCCCTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGTGCTGT ACAGGTTTTA TGGCAATTGG ATATATTTCG AAGAAGCTTG
    CGGGTTCTAA CTGGGCATGT TTGTCAGGGA GATGCCTGCA TATTTTGTGC ATTAAAGACA
    ATATTTGCAC AGTTTCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAC
    GCTCTAGCGG AAAGCTTCAA AGATGAGCAG CGATTTCAGC TGGGTCTTAT GGATGATGCT
    GCAGAATGCT TTGAAAATAT ATTGGAGAGG ATTCATTTTC ATATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACACTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCATCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCCTATGC AATGAAGTAG AAAGAATGAT GGAAAGGCAC
    GAACGTTTTA AACCTGAAAT GTTTGCAGAA TTGCTGCAAG CAGCAAATAC AACTGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT CGGTTTAGTA TGGGATTCTG AGCATTCTGA CTTGACAGAA
    GATGTTGTTC GGAATCTGGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTCAC CTAGTTGGTA TGATCTGCTA CACCAGCCGA
    CATTATTGTG CCTTTGCCTT TCATACCAAG AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGGTTGGAAC TAGATGGAAG GATGTGGTCT CCAAATGTAT CCGATGCCAT
    TTCCAGCCAC TGCTTTTGTT TTACGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAAGAT
    GCGCTTAGGC AGGTCATCAA CTGGTCACAT TATAAATCCG TTGCAGAAAA CATGGGATGT
    GAAAAGCCCT CAATTTATAA GTCAGATCAT TCAAAAGAAA ATGGATTTTG TGATCAGGCG
    AAACAGAGAG AAAATCAGAA ATTTCAAACA GATAATATTT CAGCATTTAG TCGGAGCCAT
    ATTCAAACAA GTGGTGGTGG AGGACCAGTT AAGTTAACTC ACAATGATCA AAGGGAAAAA
    ATAAAAGACA TTTCCAGAGA ATGTGCTTTA AAAGCCATTG AACAGAGGAA CTTCCTTTCA
    TCACAAAGGA AAGATTTGGA GAGGGGACAA AGAAAAGATT TGGGCCGACA TAGAGATTTG
    GTTGATGAAG ACCTTCTGCA TTTCAAGTCT GGATCACCTC CTGCCCCAAA TGGCTTTAGA
    CAATATGGAA ATCCAAATCT ATATCATAGT CAAGGAAAAG GACCATGTAA ACATGACCGA
    GTTGCCCATC AGAGCCGAGC TTCTGCACAA GTACTGAGTT CAAGTAAATC CCAAATTCTT
    GCCCCAGGAG AGAAAGTAAC TGGCAAAGTT AAGAGTGACA GTGGCACTGG ATATGATACA
    GATAGCAGCC AAGATTCTAG GGATAAAGGA AGCACCTATA GTGGCAGCAG TAAAAGCCGG
    ACCCGAGGTT GGAAACCTAT GAGAGAAACA CTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGGC AGCATAGTCC AAGACATAAA TCAAATATCA GTAACAAAGC TAAATGTAGC
    AAAGATCAGA GTTTTAACAA TTGGTCAAAA GAGAACCCAA AGCAAAAATG TTTAATGACT
    ATCTATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGGA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTCAAA GAGACTACAG TTCATGGTGA CAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGT AGTGATCATG TCAGCAATGG ATCTGCTAAT
    TTGGATTCAC CTGTTGTCGA TGGAAATGGT ACAGGAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATCCT GCAGTGACCA TGTTAGGACA AGCAACATAA ATGTAGAGTA TGTCAACTGT
    TCCTCCCAAC AGAGCAAAAA CTACTTAGAA GCCTTAGGCT TTAGAAAAGA ACTCAAGAAT
    TTGGAAGTAG GCTATAAATC TCATGAGTTC CATCCAGAGT CACATCTACA AATGAAAAAT
    CCTTTGATAA AAAGATCACA TATACGTGAA ATCAATGGAA AGTTATTCCC TTCATCAGGT
    CCACAGTTAC TCAAGGACAA TGCAGCAAGA GAGCATATCC ACCAGTCAGG TGAACAGAAA
    CTTGAAACCT CAAGTGAATA CAAATTTTCT GAGTGGCTTA ATACAGAAAA TTCTGAGAGA
    ACAGGTTTAC TTTTTCACAA TGACAATTCT GCTTCTGGAA AAAGAATGAA CAGTAATGAA
    ATAGTCTCAC CATCATTGTG GTCTTCACAC ATGAGACCTG TTGGGTTAAA GCCAGAAACT
    GCTCCCCTCA TCCAGCAGCA AACTACGATG GAACAATGTT GCTCTGAGAA CTCTCTATCC
    AGGGAACATA CAGTTCAGTC AACCTGCAGA CCTGATGGTT GTCAGATGCC AAAACTTGTT
    AGCCATAATC CACCGCCTCC TTTGCCACCA AAGAAATATG CGATAACCGG TGTGCCACAG
    TCAGAGAAAA ATGAATCCAC ACCTAATGTC AAGCTTACAG AGATGTTTAA AGCTAATTCT
    TCTAGTCTTC CAAAGCACAG TTTAAGTCTG GATTCAGAAC CAGGTTCAGA AGAGGGTACA
    TATATGAATG ATGAAAGACT TAGGGAAGCA TTTCAAGTAA AAGAGCCTGT TGGCAACACA
    CCAAACTACC TGTCCAGCTT TTCAACTGAA GATTTTCAGG CAGACTTGGC TACGGTTGTT
    GATGCATTTT GCCAACCAGA AATAGACTCT AGTTCTACCT GTCCAAATGA GACCATTTCA
    TTAACTACCT ACTTCTCAGT TGATAGCTGC ATGACAGATA CATACAGACT GAAATACCAC
    CAGAGACCCA AGCTCTGTGT TCCAGAGAAC AGTGGCTTTT GTAATGATGA TGTACTATCT
    AAGAGTGAAA GAAGGATAGC ACCTGTGTTA CACAACAGTC TTGGTTCAAG CTGTCCTTCT
    GAGCAGAGAT ATAAACCTAG TATACATTGT AATAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29931
    Clone ID Related Accession (Same CDS sequence) XM_005639323.2 , XM_005639324.2 , XM_005639323.3 , XM_022413701.1
    Accession Version XM_005639323.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3339bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005639323.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCATGGG TAAAATTCTT ACGGAAACCT AGTGGCAATC TTGGAAAAGT TTATCAACCT 
    GGAAGTATGC TATCCCTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGTGCTGT ACAGGTTTTA TGGCAATTGG ATATATTTCG AAGAAGCTTG
    CGGGTTCTAA CTGGGCATGT TTGTCAGGGA GATGCCTGCA TATTTTGTGC ATTAAAGACA
    ATATTTGCAC AGTTTCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAC
    GCTCTAGCGG AAAGCTTCAA AGATGAGCAG CGATTTCAGC TGGGTCTTAT GGATGATGCT
    GCAGAATGCT TTGAAAATAT ATTGGAGAGG ATTCATTTTC ATATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACACTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCATCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCCTATGC AATGAAGTAG AAAGAATGAT GGAAAGGCAC
    GAACGTTTTA AACCTGAAAT GTTTGCAGAA TTGCTGCAAG CAGCAAATAC AACTGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT CGGTTTAGTA TGGGATTCTG AGCATTCTGA CTTGACAGAA
    GATGTTGTTC GGAATCTGGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTCAC CTAGTTGGTA TGATCTGCTA CACCAGCCGA
    CATTATTGTG CCTTTGCCTT TCATACCAAG AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGGTTGGAAC TAGATGGAAG GATGTGGTCT CCAAATGTAT CCGATGCCAT
    TTCCAGCCAC TGCTTTTGTT TTACGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAAGAT
    GCGCTTAGGC AGGTCATCAA CTGGTCACAT TATAAATCCG TTGCAGAAAA CATGGGATGT
    GAAAAGCCCT CAATTTATAA GTCAGATCAT TCAAAAGAAA ATGGATTTTG TGATCAGGCG
    AAACAGAGAG AAAATCAGAA ATTTCAAACA GATAATATTT CAGCATTTAG TCGGAGCCAT
    ATTCAAACAA GTGGTGGTGG AGGACCAGTT AAGTTAACTC ACAATGATCA AAGGGAAAAA
    ATAAAAGACA TTTCCAGAGA ATGTGCTTTA AAAGCCATTG AACAGAGGAA CTTCCTTTCA
    TCACAAAGGA AAGATTTGGA GAGGGGACAA AGAAAAGATT TGGGCCGACA TAGAGATTTG
    GTTGATGAAG ACCTTCTGCA TTTCAAGTCT GGATCACCTC CTGCCCCAAA TGGCTTTAGA
    CAATATGGAA ATCCAAATCT ATATCATAGT CAAGGAAAAG GACCATGTAA ACATGACCGA
    GTTGCCCATC AGAGCCGAGC TTCTGCACAA GTACTGAGTT CAAGTAAATC CCAAATTCTT
    GCCCCAGGAG AGAAAGTAAC TGGCAAAGTT AAGAGTGACA GTGGCACTGG ATATGATACA
    GATAGCAGCC AAGATTCTAG GGATAAAGGA AGCACCTATA GTGGCAGCAG TAAAAGCCGG
    ACCCGAGGTT GGAAACCTAT GAGAGAAACA CTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGGC AGCATAGTCC AAGACATAAA TCAAATATCA GTAACAAAGC TAAATGTAGC
    AAAGATCAGA GTTTTAACAA TTGGTCAAAA GAGAACCCAA AGCAAAAATG TTTAATGACT
    ATCTATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGGA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTCAAA GAGACTACAG TTCATGGTGA CAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGT AGTGATCATG TCAGCAATGG ATCTGCTAAT
    TTGGATTCAC CTGTTGTCGA TGGAAATGGT ACAGGAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATCCT GCAGTGACCA TGTTAGGACA AGCAACATAA ATGTAGAGTA TGTCAACTGT
    TCCTCCCAAC AGAGCAAAAA CTACTTAGAA GCCTTAGGCT TTAGAAAAGA ACTCAAGAAT
    TTGGAAGTAG GCTATAAATC TCATGAGTTC CATCCAGAGT CACATCTACA AATGAAAAAT
    CCTTTGATAA AAAGATCACA TATACGTGAA ATCAATGGAA AGTTATTCCC TTCATCAGGT
    CCACAGTTAC TCAAGGACAA TGCAGCAAGA GAGCATATCC ACCAGTCAGG TGAACAGAAA
    CTTGAAACCT CAAGTGAATA CAAATTTTCT GAGTGGCTTA ATACAGAAAA TTCTGAGAGA
    ACAGGTTTAC TTTTTCACAA TGACAATTCT GCTTCTGGAA AAAGAATGAA CAGTAATGAA
    ATAGTCTCAC CATCATTGTG GTCTTCACAC ATGAGACCTG TTGGGTTAAA GCCAGAAACT
    GCTCCCCTCA TCCAGCAGCA AACTACGATG GAACAATGTT GCTCTGAGAA CTCTCTATCC
    AGGGAACATA CAGTTCAGTC AACCTGCAGA CCTGATGGTT GTCAGATGCC AAAACTTGTT
    AGCCATAATC CACCGCCTCC TTTGCCACCA AAGAAATATG CGATAACCGG TGTGCCACAG
    TCAGAGAAAA ATGAATCCAC ACCTAATGTC AAGCTTACAG AGATGTTTAA AGCTAATTCT
    TCTAGTCTTC CAAAGCACAG TTTAAGTCTG GATTCAGAAC CAGGTTCAGA AGAGGGTACA
    TATATGAATG ATGAAAGACT TAGGGAAGCA TTTCAAGTAA AAGAGCCTGT TGGCAACACA
    CCAAACTACC TGTCCAGCTT TTCAACTGAA GATTTTCAGG CAGACTTGGC TACGGTTGTT
    GATGCATTTT GCCAACCAGA AATAGACTCT AGTTCTACCT GTCCAAATGA GACCATTTCA
    TTAACTACCT ACTTCTCAGT TGATAGCTGC ATGACAGATA CATACAGACT GAAATACCAC
    CAGAGACCCA AGCTCTGTGT TCCAGAGAAC AGTGGCTTTT GTAATGATGA TGTACTATCT
    AAGAGTGAAA GAAGGATAGC ACCTGTGTTA CACAACAGTC TTGGTTCAAG CTGTCCTTCT
    GAGCAGAGAT ATAAACCTAG TATACATTGT AATAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005639380.1
    CDS681..4019
    Translation

    Target ORF information:

    RefSeq Version XM_005639323.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ubiquitin specific peptidase 53 (USP53), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005639323.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCATGGG TAAAATTCTT ACGGAAACCT AGTGGCAATC TTGGAAAAGT TTATCAACCT 
    GGAAGTATGC TATCCCTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGTGCTGT ACAGGTTTTA TGGCAATTGG ATATATTTCG AAGAAGCTTG
    CGGGTTCTAA CTGGGCATGT TTGTCAGGGA GATGCCTGCA TATTTTGTGC ATTAAAGACA
    ATATTTGCAC AGTTTCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAC
    GCTCTAGCGG AAAGCTTCAA AGATGAGCAG CGATTTCAGC TGGGTCTTAT GGATGATGCT
    GCAGAATGCT TTGAAAATAT ATTGGAGAGG ATTCATTTTC ATATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACACTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCATCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCCTATGC AATGAAGTAG AAAGAATGAT GGAAAGGCAC
    GAACGTTTTA AACCTGAAAT GTTTGCAGAA TTGCTGCAAG CAGCAAATAC AACTGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT CGGTTTAGTA TGGGATTCTG AGCATTCTGA CTTGACAGAA
    GATGTTGTTC GGAATCTGGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTCAC CTAGTTGGTA TGATCTGCTA CACCAGCCGA
    CATTATTGTG CCTTTGCCTT TCATACCAAG AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGGTTGGAAC TAGATGGAAG GATGTGGTCT CCAAATGTAT CCGATGCCAT
    TTCCAGCCAC TGCTTTTGTT TTACGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAAGAT
    GCGCTTAGGC AGGTCATCAA CTGGTCACAT TATAAATCCG TTGCAGAAAA CATGGGATGT
    GAAAAGCCCT CAATTTATAA GTCAGATCAT TCAAAAGAAA ATGGATTTTG TGATCAGGCG
    AAACAGAGAG AAAATCAGAA ATTTCAAACA GATAATATTT CAGCATTTAG TCGGAGCCAT
    ATTCAAACAA GTGGTGGTGG AGGACCAGTT AAGTTAACTC ACAATGATCA AAGGGAAAAA
    ATAAAAGACA TTTCCAGAGA ATGTGCTTTA AAAGCCATTG AACAGAGGAA CTTCCTTTCA
    TCACAAAGGA AAGATTTGGA GAGGGGACAA AGAAAAGATT TGGGCCGACA TAGAGATTTG
    GTTGATGAAG ACCTTCTGCA TTTCAAGTCT GGATCACCTC CTGCCCCAAA TGGCTTTAGA
    CAATATGGAA ATCCAAATCT ATATCATAGT CAAGGAAAAG GACCATGTAA ACATGACCGA
    GTTGCCCATC AGAGCCGAGC TTCTGCACAA GTACTGAGTT CAAGTAAATC CCAAATTCTT
    GCCCCAGGAG AGAAAGTAAC TGGCAAAGTT AAGAGTGACA GTGGCACTGG ATATGATACA
    GATAGCAGCC AAGATTCTAG GGATAAAGGA AGCACCTATA GTGGCAGCAG TAAAAGCCGG
    ACCCGAGGTT GGAAACCTAT GAGAGAAACA CTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGGC AGCATAGTCC AAGACATAAA TCAAATATCA GTAACAAAGC TAAATGTAGC
    AAAGATCAGA GTTTTAACAA TTGGTCAAAA GAGAACCCAA AGCAAAAATG TTTAATGACT
    ATCTATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGGA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTCAAA GAGACTACAG TTCATGGTGA CAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGT AGTGATCATG TCAGCAATGG ATCTGCTAAT
    TTGGATTCAC CTGTTGTCGA TGGAAATGGT ACAGGAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATCCT GCAGTGACCA TGTTAGGACA AGCAACATAA ATGTAGAGTA TGTCAACTGT
    TCCTCCCAAC AGAGCAAAAA CTACTTAGAA GCCTTAGGCT TTAGAAAAGA ACTCAAGAAT
    TTGGAAGTAG GCTATAAATC TCATGAGTTC CATCCAGAGT CACATCTACA AATGAAAAAT
    CCTTTGATAA AAAGATCACA TATACGTGAA ATCAATGGAA AGTTATTCCC TTCATCAGGT
    CCACAGTTAC TCAAGGACAA TGCAGCAAGA GAGCATATCC ACCAGTCAGG TGAACAGAAA
    CTTGAAACCT CAAGTGAATA CAAATTTTCT GAGTGGCTTA ATACAGAAAA TTCTGAGAGA
    ACAGGTTTAC TTTTTCACAA TGACAATTCT GCTTCTGGAA AAAGAATGAA CAGTAATGAA
    ATAGTCTCAC CATCATTGTG GTCTTCACAC ATGAGACCTG TTGGGTTAAA GCCAGAAACT
    GCTCCCCTCA TCCAGCAGCA AACTACGATG GAACAATGTT GCTCTGAGAA CTCTCTATCC
    AGGGAACATA CAGTTCAGTC AACCTGCAGA CCTGATGGTT GTCAGATGCC AAAACTTGTT
    AGCCATAATC CACCGCCTCC TTTGCCACCA AAGAAATATG CGATAACCGG TGTGCCACAG
    TCAGAGAAAA ATGAATCCAC ACCTAATGTC AAGCTTACAG AGATGTTTAA AGCTAATTCT
    TCTAGTCTTC CAAAGCACAG TTTAAGTCTG GATTCAGAAC CAGGTTCAGA AGAGGGTACA
    TATATGAATG ATGAAAGACT TAGGGAAGCA TTTCAAGTAA AAGAGCCTGT TGGCAACACA
    CCAAACTACC TGTCCAGCTT TTCAACTGAA GATTTTCAGG CAGACTTGGC TACGGTTGTT
    GATGCATTTT GCCAACCAGA AATAGACTCT AGTTCTACCT GTCCAAATGA GACCATTTCA
    TTAACTACCT ACTTCTCAGT TGATAGCTGC ATGACAGATA CATACAGACT GAAATACCAC
    CAGAGACCCA AGCTCTGTGT TCCAGAGAAC AGTGGCTTTT GTAATGATGA TGTACTATCT
    AAGAGTGAAA GAAGGATAGC ACCTGTGTTA CACAACAGTC TTGGTTCAAG CTGTCCTTCT
    GAGCAGAGAT ATAAACCTAG TATACATTGT AATAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29931
    Clone ID Related Accession (Same CDS sequence) XM_005639323.2 , XM_005639324.2 , XM_005639323.3 , XM_022413701.1
    Accession Version XM_022413701.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3339bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCATGGG TAAAATTCTT ACGGAAACCT AGTGGCAATC TTGGAAAAGT TTATCAACCT 
    GGAAGTATGC TATCCCTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGTGCTGT ACAGGTTTTA TGGCAATTGG ATATATTTCG AAGAAGCTTG
    CGGGTTCTAA CTGGGCATGT TTGTCAGGGA GATGCCTGCA TATTTTGTGC ATTAAAGACA
    ATATTTGCAC AGTTTCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAC
    GCTCTAGCGG AAAGCTTCAA AGATGAGCAG CGATTTCAGC TGGGTCTTAT GGATGATGCT
    GCAGAATGCT TTGAAAATAT ATTGGAGAGG ATTCATTTTC ATATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACACTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCATCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCCTATGC AATGAAGTAG AAAGAATGAT GGAAAGGCAC
    GAACGTTTTA AACCTGAAAT GTTTGCAGAA TTGCTGCAAG CAGCAAATAC AACTGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT CGGTTTAGTA TGGGATTCTG AGCATTCTGA CTTGACAGAA
    GATGTTGTTC GGAATCTGGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTCAC CTAGTTGGTA TGATCTGCTA CACCAGCCGA
    CATTATTGTG CCTTTGCCTT TCATACCAAG AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGGTTGGAAC TAGATGGAAG GATGTGGTCT CCAAATGTAT CCGATGCCAT
    TTCCAGCCAC TGCTTTTGTT TTACGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAAGAT
    GCGCTTAGGC AGGTCATCAA CTGGTCACAT TATAAATCCG TTGCAGAAAA CATGGGATGT
    GAAAAGCCCT CAATTTATAA GTCAGATCAT TCAAAAGAAA ATGGATTTTG TGATCAGGCG
    AAACAGAGAG AAAATCAGAA ATTTCAAACA GATAATATTT CAGCATTTAG TCGGAGCCAT
    ATTCAAACAA GTGGTGGTGG AGGACCAGTT AAGTTAACTC ACAATGATCA AAGGGAAAAA
    ATAAAAGACA TTTCCAGAGA ATGTGCTTTA AAAGCCATTG AACAGAGGAA CTTCCTTTCA
    TCACAAAGGA AAGATTTGGA GAGGGGACAA AGAAAAGATT TGGGCCGACA TAGAGATTTG
    GTTGATGAAG ACCTTCTGCA TTTCAAGTCT GGATCACCTC CTGCCCCAAA TGGCTTTAGA
    CAATATGGAA ATCCAAATCT ATATCATAGT CAAGGAAAAG GACCATGTAA ACATGACCGA
    GTTGCCCATC AGAGCCGAGC TTCTGCACAA GTACTGAGTT CAAGTAAATC CCAAATTCTT
    GCCCCAGGAG AGAAAGTAAC TGGCAAAGTT AAGAGTGACA GTGGCACTGG ATATGATACA
    GATAGCAGCC AAGATTCTAG GGATAAAGGA AGCACCTATA GTGGCAGCAG TAAAAGCCGG
    ACCCGAGGTT GGAAACCTAT GAGAGAAACA CTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGGC AGCATAGTCC AAGACATAAA TCAAATATCA GTAACAAAGC TAAATGTAGC
    AAAGATCAGA GTTTTAACAA TTGGTCAAAA GAGAACCCAA AGCAAAAATG TTTAATGACT
    ATCTATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGGA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTCAAA GAGACTACAG TTCATGGTGA CAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGT AGTGATCATG TCAGCAATGG ATCTGCTAAT
    TTGGATTCAC CTGTTGTCGA TGGAAATGGT ACAGGAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATCCT GCAGTGACCA TGTTAGGACA AGCAACATAA ATGTAGAGTA TGTCAACTGT
    TCCTCCCAAC AGAGCAAAAA CTACTTAGAA GCCTTAGGCT TTAGAAAAGA ACTCAAGAAT
    TTGGAAGTAG GCTATAAATC TCATGAGTTC CATCCAGAGT CACATCTACA AATGAAAAAT
    CCTTTGATAA AAAGATCACA TATACGTGAA ATCAATGGAA AGTTATTCCC TTCATCAGGT
    CCACAGTTAC TCAAGGACAA TGCAGCAAGA GAGCATATCC ACCAGTCAGG TGAACAGAAA
    CTTGAAACCT CAAGTGAATA CAAATTTTCT GAGTGGCTTA ATACAGAAAA TTCTGAGAGA
    ACAGGTTTAC TTTTTCACAA TGACAATTCT GCTTCTGGAA AAAGAATGAA CAGTAATGAA
    ATAGTCTCAC CATCATTGTG GTCTTCACAC ATGAGACCTG TTGGGTTAAA GCCAGAAACT
    GCTCCCCTCA TCCAGCAGCA AACTACGATG GAACAATGTT GCTCTGAGAA CTCTCTATCC
    AGGGAACATA CAGTTCAGTC AACCTGCAGA CCTGATGGTT GTCAGATGCC AAAACTTGTT
    AGCCATAATC CACCGCCTCC TTTGCCACCA AAGAAATATG CGATAACCGG TGTGCCACAG
    TCAGAGAAAA ATGAATCCAC ACCTAATGTC AAGCTTACAG AGATGTTTAA AGCTAATTCT
    TCTAGTCTTC CAAAGCACAG TTTAAGTCTG GATTCAGAAC CAGGTTCAGA AGAGGGTACA
    TATATGAATG ATGAAAGACT TAGGGAAGCA TTTCAAGTAA AAGAGCCTGT TGGCAACACA
    CCAAACTACC TGTCCAGCTT TTCAACTGAA GATTTTCAGG CAGACTTGGC TACGGTTGTT
    GATGCATTTT GCCAACCAGA AATAGACTCT AGTTCTACCT GTCCAAATGA GACCATTTCA
    TTAACTACCT ACTTCTCAGT TGATAGCTGC ATGACAGATA CATACAGACT GAAATACCAC
    CAGAGACCCA AGCTCTGTGT TCCAGAGAAC AGTGGCTTTT GTAATGATGA TGTACTATCT
    AAGAGTGAAA GAAGGATAGC ACCTGTGTTA CACAACAGTC TTGGTTCAAG CTGTCCTTCT
    GAGCAGAGAT ATAAACCTAG TATACATTGT AATAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269409.1
    CDS808..4146
    Translation

    Target ORF information:

    RefSeq Version XM_022413701.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ubiquitin specific peptidase 53 (USP53), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413701.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCATGGG TAAAATTCTT ACGGAAACCT AGTGGCAATC TTGGAAAAGT TTATCAACCT 
    GGAAGTATGC TATCCCTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGTGCTGT ACAGGTTTTA TGGCAATTGG ATATATTTCG AAGAAGCTTG
    CGGGTTCTAA CTGGGCATGT TTGTCAGGGA GATGCCTGCA TATTTTGTGC ATTAAAGACA
    ATATTTGCAC AGTTTCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAC
    GCTCTAGCGG AAAGCTTCAA AGATGAGCAG CGATTTCAGC TGGGTCTTAT GGATGATGCT
    GCAGAATGCT TTGAAAATAT ATTGGAGAGG ATTCATTTTC ATATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACACTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCATCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCCTATGC AATGAAGTAG AAAGAATGAT GGAAAGGCAC
    GAACGTTTTA AACCTGAAAT GTTTGCAGAA TTGCTGCAAG CAGCAAATAC AACTGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT CGGTTTAGTA TGGGATTCTG AGCATTCTGA CTTGACAGAA
    GATGTTGTTC GGAATCTGGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTCAC CTAGTTGGTA TGATCTGCTA CACCAGCCGA
    CATTATTGTG CCTTTGCCTT TCATACCAAG AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGGTTGGAAC TAGATGGAAG GATGTGGTCT CCAAATGTAT CCGATGCCAT
    TTCCAGCCAC TGCTTTTGTT TTACGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAAGAT
    GCGCTTAGGC AGGTCATCAA CTGGTCACAT TATAAATCCG TTGCAGAAAA CATGGGATGT
    GAAAAGCCCT CAATTTATAA GTCAGATCAT TCAAAAGAAA ATGGATTTTG TGATCAGGCG
    AAACAGAGAG AAAATCAGAA ATTTCAAACA GATAATATTT CAGCATTTAG TCGGAGCCAT
    ATTCAAACAA GTGGTGGTGG AGGACCAGTT AAGTTAACTC ACAATGATCA AAGGGAAAAA
    ATAAAAGACA TTTCCAGAGA ATGTGCTTTA AAAGCCATTG AACAGAGGAA CTTCCTTTCA
    TCACAAAGGA AAGATTTGGA GAGGGGACAA AGAAAAGATT TGGGCCGACA TAGAGATTTG
    GTTGATGAAG ACCTTCTGCA TTTCAAGTCT GGATCACCTC CTGCCCCAAA TGGCTTTAGA
    CAATATGGAA ATCCAAATCT ATATCATAGT CAAGGAAAAG GACCATGTAA ACATGACCGA
    GTTGCCCATC AGAGCCGAGC TTCTGCACAA GTACTGAGTT CAAGTAAATC CCAAATTCTT
    GCCCCAGGAG AGAAAGTAAC TGGCAAAGTT AAGAGTGACA GTGGCACTGG ATATGATACA
    GATAGCAGCC AAGATTCTAG GGATAAAGGA AGCACCTATA GTGGCAGCAG TAAAAGCCGG
    ACCCGAGGTT GGAAACCTAT GAGAGAAACA CTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGGC AGCATAGTCC AAGACATAAA TCAAATATCA GTAACAAAGC TAAATGTAGC
    AAAGATCAGA GTTTTAACAA TTGGTCAAAA GAGAACCCAA AGCAAAAATG TTTAATGACT
    ATCTATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGGA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTCAAA GAGACTACAG TTCATGGTGA CAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGT AGTGATCATG TCAGCAATGG ATCTGCTAAT
    TTGGATTCAC CTGTTGTCGA TGGAAATGGT ACAGGAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATCCT GCAGTGACCA TGTTAGGACA AGCAACATAA ATGTAGAGTA TGTCAACTGT
    TCCTCCCAAC AGAGCAAAAA CTACTTAGAA GCCTTAGGCT TTAGAAAAGA ACTCAAGAAT
    TTGGAAGTAG GCTATAAATC TCATGAGTTC CATCCAGAGT CACATCTACA AATGAAAAAT
    CCTTTGATAA AAAGATCACA TATACGTGAA ATCAATGGAA AGTTATTCCC TTCATCAGGT
    CCACAGTTAC TCAAGGACAA TGCAGCAAGA GAGCATATCC ACCAGTCAGG TGAACAGAAA
    CTTGAAACCT CAAGTGAATA CAAATTTTCT GAGTGGCTTA ATACAGAAAA TTCTGAGAGA
    ACAGGTTTAC TTTTTCACAA TGACAATTCT GCTTCTGGAA AAAGAATGAA CAGTAATGAA
    ATAGTCTCAC CATCATTGTG GTCTTCACAC ATGAGACCTG TTGGGTTAAA GCCAGAAACT
    GCTCCCCTCA TCCAGCAGCA AACTACGATG GAACAATGTT GCTCTGAGAA CTCTCTATCC
    AGGGAACATA CAGTTCAGTC AACCTGCAGA CCTGATGGTT GTCAGATGCC AAAACTTGTT
    AGCCATAATC CACCGCCTCC TTTGCCACCA AAGAAATATG CGATAACCGG TGTGCCACAG
    TCAGAGAAAA ATGAATCCAC ACCTAATGTC AAGCTTACAG AGATGTTTAA AGCTAATTCT
    TCTAGTCTTC CAAAGCACAG TTTAAGTCTG GATTCAGAAC CAGGTTCAGA AGAGGGTACA
    TATATGAATG ATGAAAGACT TAGGGAAGCA TTTCAAGTAA AAGAGCCTGT TGGCAACACA
    CCAAACTACC TGTCCAGCTT TTCAACTGAA GATTTTCAGG CAGACTTGGC TACGGTTGTT
    GATGCATTTT GCCAACCAGA AATAGACTCT AGTTCTACCT GTCCAAATGA GACCATTTCA
    TTAACTACCT ACTTCTCAGT TGATAGCTGC ATGACAGATA CATACAGACT GAAATACCAC
    CAGAGACCCA AGCTCTGTGT TCCAGAGAAC AGTGGCTTTT GTAATGATGA TGTACTATCT
    AAGAGTGAAA GAAGGATAGC ACCTGTGTTA CACAACAGTC TTGGTTCAAG CTGTCCTTCT
    GAGCAGAGAT ATAAACCTAG TATACATTGT AATAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.