ZGRF1 cDNA ORF clone, Canis lupus familiaris(dog)

The following ZGRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZGRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe284016 XM_022413417.1
Latest version!
Canis lupus familiaris zinc finger GRF-type containing 1 (ZGRF1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe284017 XM_022413418.1
Latest version!
Canis lupus familiaris zinc finger GRF-type containing 1 (ZGRF1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe284018 XM_022413419.1
Latest version!
Canis lupus familiaris zinc finger GRF-type containing 1 (ZGRF1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe44625 XM_014110062.1
Latest version!
Canis lupus familiaris zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe284011 XM_022413412.1
Latest version!
Canis lupus familiaris zinc finger GRF-type containing 1 (ZGRF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe284012 XM_022413413.1
Latest version!
Canis lupus familiaris zinc finger GRF-type containing 1 (ZGRF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe284013 XM_022413414.1
Latest version!
Canis lupus familiaris zinc finger GRF-type containing 1 (ZGRF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe284014 XM_022413415.1
Latest version!
Canis lupus familiaris zinc finger GRF-type containing 1 (ZGRF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe284015 XM_022413416.1
Latest version!
Canis lupus familiaris zinc finger GRF-type containing 1 (ZGRF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe44624 XM_014110061.2
Latest version!
Canis lupus familiaris zinc finger GRF-type containing 1 (ZGRF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe44624 XM_022413411.1
Latest version!
Canis lupus familiaris zinc finger GRF-type containing 1 (ZGRF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
View Old Accession Versions >>
OCe44624 XM_014110061.1 Canis lupus familiaris zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe284016
    Clone ID Related Accession (Same CDS sequence) XM_022413417.1
    Accession Version XM_022413417.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5964bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein ZGRF1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGAAAGCC AGGAATTTAT TGTACTGTAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
    TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAGTTTT ATATGATGAC
    AAAGGAGAAT GTTTGGAGAG TATATTTCTT AAGTGCCTTG AGGTGAAGCC TGGAGATGAC
    TTAGAAGGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATTGCT
    ATTAAACCGG ATGTCATTAA AGAAACATCA GAGTTAAATC CAAGGAAATT TATATCCTCT
    GGGCGGTCCC TTGGATGTCA GCCTGCTGGT TTAAAAAGGA AGTTTACTGG TTTTCAAGGA
    CCACGTCAAG TGCCAAGGAA AATGGTTATT ACAGAAAATG ATGAATCAGC TGCATCACCT
    GAGGCTAAGA AACCTGGCCC TATTTTTCTT TCTCCCTTCT ACAACACACC TCCTTTGTTT
    TCTACTGCTG GCAAGAAAGA TACAAATAAT ATGCCAACAG ACCCTGAGAA CAGTGTCATT
    TACAAGAACA GAGAGAAAAA TGGCTTATAT TATTCTTCTG TTGTCTCTAC TCCATCCTTT
    AAGAATAACC CAGAAATGCT ATGTGAAGAA AATTATTTTT GCTCACCTGT CTATTCTGTA
    AATAAGCATT CAGATTCTTT ACTATCCAAT GAGGCCATAA AAAAAGATGG TTTGACATCT
    CACTGTTTAG GAGTTTCAGA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTAAAG
    TCCAAATCAA CTAGTATGGG TAAAGAACTA GATTCTGGGA TTACAGAACA TTTCCCTCAG
    ATACAACTAC AAGAAAGTGT AAACATTCCT TCTAAACCAA AGTGCATAAT TGAACAAGAC
    AAGTGTGGTG AGGTGAAGAG CACAGACAAT TTATACTACC AGCATCAATC GGAAAATACT
    ATGAGCTATA AAAGCAGATG GGCCATGTAT TTATCCTCAC AGAGTTCACC TGTACGTTCT
    TCTACTGAAG ATGGAAATAA TATAGAAAGG AAACCCAAAA CTCAGGGAGA TAATGTAAAT
    TTAAATTTGA AAGACCTTGT ACAAAAAAGG ATACAGTTTT TTGAAACATG TACTGAAAAG
    GGTAAAAACT ATAATGAAGA CAAGCCAGTA GGTGATAATG ATCAATCTTG CGATCAGGAA
    GTAAAATTGG AAATTCCTTC ATTCTGTAAA AGCAACAGCT TACCATTTAC CTGTAGCAGT
    GTAGAGAATG ATGGCTTATT ATCAGAATCT GACATTCAAA AAAATAATAA AATACCTGTT
    AGTCAGGATG ACCAGATGTG TCTAAAAGGA TCAATTCTCA TTAGAGAAAA TGCTCAGAAG
    ATAAATATAT GTGGAACCCC AGAAAAGTAT GACCAACCCA TGTCGTCCCT GCCAGAATCT
    GAACATCTAC AAATTGAGTC TTCTACAAGT AACAATTCTA GGATCTCTGA TGACTTTACC
    AATATGCTTT CTAAAAGTAA TACTGATAAT GAAAATCTTA ATAGTATTCC TGAACCAATG
    AGTAATGTAA CACAGCCACT TGTGGAGGTG ACTTTTAATC TGAACAATTT TGAAACCAGT
    GACTCTGAGG AGGAATCACA AGAAAATAAC AAAATTTCCC AGGATTCAGA GGGTTGGGTA
    AAGGAAACTC TGGTTAATGA TGGCAGTTCA AGTGTTCAAA AGAGATGTGA AAATATAAGC
    TGTAAGAAAG TTGGCAGTGA ACATTTGTCT CATTTAACAT CTATGGAGGA TAAGCCTACA
    GAGACATTTC CTAAAGAGAC TCTGCTGTCA CAGTTTTGTG ATAAAGCGTG TATGGGTTTT
    GACACAGGAC CTTGGAAAAC TGGAAACAGA AAAGAAATAG AAGAGTATAA TGACACATTA
    AGCAGTTTTG ATTCATCTTT AGAGTGGACT GATGATGTAT ATGTAGATAA TAATGAAGAT
    GCTAATAAAT CTATACAAAA AGTCAGAATT AACTATGATA TTGCTTCACT ACCGAGTAAG
    TCTAAAGGTA TAAGCTCAAA TTTACATATT CCTCAGTTAA ACATAGCCAC TAATCAGATT
    CCTAAAAACA GCCTATTCTC GGAACAGTCT GGGCTTAAGC CTTTTATAAT GGGAAATAAA
    TTAGACAAAA ATGATGAACA GGTTTTACTG TCAACTTCTA GCAGTGACAT CAGTATCCAA
    CAATTAAATG TTAATCAGAA TCACTCTGAA GAACCTGTTG CTCTTGGTAA ATCAAATACC
    CAAGTTTCCA ATTCTTTGTT GTACCTTCTC GGAGAAAAGC ATCCTATTTC CAAAGAAACA
    GAAGCGTGTA TTCCTGAATC CGAAGATTTG GGAAGGATTA GAAGTTTAGA CCATGACCAT
    ATTGAAGTAG AACCTGCTGG AGAAAGCAAA CAACATTGGA ATAGTCCTAG GAATCCTTCA
    GAACTCTCTG GATTAATAAA TAACATTTCC CTTTTAAAGT CATTGTCTGA ACATAGTACA
    GCTTTAGAAG GCTTGGAAAT ATTGAAAAAG AAAAATACTA CCTTTAAACA ACAAGGAACT
    CTACAGACAT ATAATCCAGA TAGTAATTCT GAAGCTAGGA AACGATCGAC TGCAGAAGTT
    TTGCAAGCTT TACCAAAGTT GCCTCCTCTG AACCAGGATT CTCAACAGAT TATAAAAGAA
    AATGAAGTTG AACCAAGTAA ACCACTTCAG TCTTTGCAGT TCTTTTCCTT TGGAGATAAA
    GAGACTGCTT TTCTAGATGT TATGCCTAAA CAATCAGAGA GAAAAACCTG TGACCCTAAG
    CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA GGATCTGCTA CTAGTGCTGT AATGATGAGA
    GGACACAGCT CACAGCTAGA ATGCGGTCAA TTTCCAGATA GTATTGAGTA TGAAAACTGC
    ACAAGAGACA CTTGTTTATT GACACCAAAA TTGACCAGTG CTTGTATGCA GATTGATTTC
    CTGCAGTTCC CAGATGAAAG AAACATTCCA AAAGAAGATA TTAGCTTTAA TATTGAAGAC
    AATTTTCAAG TGAGAGCTGA GTCAAATAAG GACTCAATTG AAAATGACTT GATTTTGGAT
    TTCTCTCCTA AAAATTCAGA ACATTTCTAT CATTATTTGG ATTCTAGTTC TAAATCAGCA
    GAGAAGACTT CATGGGAAGC AAATAAGGTA ACATCACCAG AACAGAAGAT CTCTACATTA
    AACCCTGATT CTACTTTTTC TTTGAACTCA CGAGATGAAG ACTTTGTGCT AGAATTCTCT
    GAGGAGTCCC TGAAAGAAAG AACTTTACCT GTTATCAAAA AATCCAGTTC TTTGGAGAAT
    AAGAACTTTC AAAGGCTTCC ATTACTCAGT TTTGCCAATC AGATCATTGT TAAGGAGATA
    AATGCCTCTG AGCTATGCTT CCCAAGTGGG CAGAAAATAA AATCTGCTTA TCTTCCCCAA
    AGGCAGATTC ATATACCAGC TGTTTTTCAG TCTCCTGCTC ATTATAAGCA GATTTTTACA
    TCTTGTCTCA TAGAACACTT AAATATATTG TTGTTTGGGT TGGCACAGAG GTTGCACAAA
    GCTCTTTCAA AAGTTGACAT ATCATTTTAC ACATCATTAA AGGGAGAGAA ACTAAAAAAT
    GTAGAAAGTA ATGTACCATC CTGCCATCAT CATCAACCTG CAAAACTTGT CATGGTTAAA
    AAGGAAGGTC CAAATAAGGG TCGTCTTTTT TATACCTGTG ATGGACCCAA AGCTGACCGA
    TGTAAATTTT TCAAGTGGCT TGAAGAAGTG ACCCCAGGAT ATTTGACACA AGAAGAGTCT
    CTACCTAGCA TAGTTTTAAG TGATATAAAG AGTATTGGCT TATACTTAAG AAGTCAAAAT
    ATACCCCTCT TTGAGGAATG TCAGCTTTTG GTGAGGAAAG GATTTGATTT TCAAAGAAAA
    CAGTATGGCA AACTGAAGAA GTTTAATGCT GTAAATCCTG AATTTTATAG TGAACCTAAA
    AGCAAACTTT ATCTTAAGCT GAGTCGGAGG GAAAGTTCTT CAACTTACAG CAAAAATGAT
    CTTTGGGTGG TTTCAAAAAC ATTGGACTTT GAATTGGATA CTTTTATTGC GTGTAGTGCT
    TTCTTTGGAC CATCATCTAT CAATGAGGTG GAGCTACTAC CTTTGAAAGG TTATTTCCCC
    TCTAACTGGC CCACTAACAT AGTGGTACAT GCATTGTTGG TTTGTAATGC TAGCACTGAG
    CTGACAACTT TGAAAAACAT TCAGGACTAC TTTAATCCAG CTACTCTACC TCTAACACAG
    TACTTGTTAA CAATGTCTTC ATCAACTATA GTTAGCAACA AGAGAGTCAA TAAGAGAAAA
    TTTATCCCAC CAGCCTTCTC AAATATCAAT ACAAAATTTG AACTTCTCAG CCTAGAAGCC
    ACATTACAGT TAGCTAGTGA ATTAATTCAG GCACACAACT TAAATAAGGA TCAAGCTACA
    GCTTTAATTC AGATAGCTCA AATGATGGCA TCACATGAAA ATGCTGAAGA AGTGAAGAAA
    GTACAAACCC ATACCCTCCC TATCACAATC ATACATGGTG TTTTTGGAGC AGGAAAGAGT
    TATTTGTTGG CAGTGGTGAT TTTGTTTTTT GTACAGCTAT TTGAAAAGAG TGAAGTTCTC
    ACAGTTGGAA ATGTAAGACC TTGGAAACTT CTCATTTCTT CTTCCACTAA TGTAGCTGTG
    GACAGAGTAC TTCTTGGGCT TCTCAGTCTT GGTTTTGAAA AGTTCGTCAG AGTTGGGAGT
    GTCAGGAAGA TTGCCAAGCC AATTTTACCT TATAGTTTGC ATGCTGGCTC AGAAAATGAA
    AATGAACAAC TAAAAGAACT ACATGGACTA ATGAAAGAAG ACTTGACTCC TCTGGAAAGG
    GTCTATGTCA GAAAAAGTAT TGAGCAGCAT AAACTGGGGA CCAATAAAAC CCTGCTGAAG
    CAGGTACGAG TAGTTGGAGT TACCTGTGCA GCTTGCCCAT TCCCATGTAT GAATGATCTT
    AAATTTCCTG TAGTTGTGTT GGATGAGTGT AGTCAAATAA CTGAACCGGC CTCTCTCCTT
    CCTATTGCAA GGTTTGAGTG TGAAAAGCTG ATTCTTGTTG GAGATCCTAA ACAGCTTTCT
    CCTACTATTC AGGGTTCTGA TGCAGCCCAT GAAAATGGAT TGGAACAAAC TCTTTTTGAT
    CGGCTTTGCT TAATGGGCCA TAAGCCAGTT CTGTTGAGAA CCCAGTACCG TTGTCACCCT
    GCAATCAGTG ATATCTCTAA TGATCTGTTT TATGAAGGAA ACCTTGTGGA TGGCATTTCT
    GAAACAGAGA GGGGCCCTTT ATTGGAATGG CTCCCAACAC TATGTTTCTA TAACATTAAA
    GGACCTGAAC AGATTGAAAG AGATAACAGC TTTCATAATG TGGCAGAAGC TGCTTTTACA
    CTCAAGCTGA TTCAATCACT GATTGCGAGT GGGATAGCGG GCTCTATGAT TGGGGTGATA
    ACATTATACA AATCCCAGAT GTACAAGCTT TGTCATTTAC TCAGTGCCGT GGACTTTGAC
    AATCCTGATA TTAAAGCTGT GCAGGTATCC ACAGTAGATG CTTTTCAGGG AGCTGAAAAG
    GAGATCATTA TTCTGTCCTG TGTAAGGACA AGACAAGTAG GATTTATTGA TTCAGAAAAA
    AGAATGAATG TTGCACTGAC CAGAGGAAGG AGGCATTTAT TGATCGTGGG AAATTTAGCC
    TGTTTGCGGA AAAATCGACT CTGGGGACGT GTAATCCAAC ACTGTGAAGG AAGGAGAGAT
    GGATTACAAC ATGCAAGCCA GTGTGAACCA CAGCTGAACC ATCTTCTTAA AGAGTATTTT
    GAAAAACGAG CAGAAGAAAA GCAGAAAAAA AGTGAAAAAG ATAAATCTAA AGATAAATCA
    CATTCACAAA AAGATATGGT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269125.1
    CDS314..6277
    Translation

    Target ORF information:

    RefSeq Version XM_022413417.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris zinc finger GRF-type containing 1 (ZGRF1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413417.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGAAAGCC AGGAATTTAT TGTACTGTAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
    TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAGTTTT ATATGATGAC
    AAAGGAGAAT GTTTGGAGAG TATATTTCTT AAGTGCCTTG AGGTGAAGCC TGGAGATGAC
    TTAGAAGGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATTGCT
    ATTAAACCGG ATGTCATTAA AGAAACATCA GAGTTAAATC CAAGGAAATT TATATCCTCT
    GGGCGGTCCC TTGGATGTCA GCCTGCTGGT TTAAAAAGGA AGTTTACTGG TTTTCAAGGA
    CCACGTCAAG TGCCAAGGAA AATGGTTATT ACAGAAAATG ATGAATCAGC TGCATCACCT
    GAGGCTAAGA AACCTGGCCC TATTTTTCTT TCTCCCTTCT ACAACACACC TCCTTTGTTT
    TCTACTGCTG GCAAGAAAGA TACAAATAAT ATGCCAACAG ACCCTGAGAA CAGTGTCATT
    TACAAGAACA GAGAGAAAAA TGGCTTATAT TATTCTTCTG TTGTCTCTAC TCCATCCTTT
    AAGAATAACC CAGAAATGCT ATGTGAAGAA AATTATTTTT GCTCACCTGT CTATTCTGTA
    AATAAGCATT CAGATTCTTT ACTATCCAAT GAGGCCATAA AAAAAGATGG TTTGACATCT
    CACTGTTTAG GAGTTTCAGA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTAAAG
    TCCAAATCAA CTAGTATGGG TAAAGAACTA GATTCTGGGA TTACAGAACA TTTCCCTCAG
    ATACAACTAC AAGAAAGTGT AAACATTCCT TCTAAACCAA AGTGCATAAT TGAACAAGAC
    AAGTGTGGTG AGGTGAAGAG CACAGACAAT TTATACTACC AGCATCAATC GGAAAATACT
    ATGAGCTATA AAAGCAGATG GGCCATGTAT TTATCCTCAC AGAGTTCACC TGTACGTTCT
    TCTACTGAAG ATGGAAATAA TATAGAAAGG AAACCCAAAA CTCAGGGAGA TAATGTAAAT
    TTAAATTTGA AAGACCTTGT ACAAAAAAGG ATACAGTTTT TTGAAACATG TACTGAAAAG
    GGTAAAAACT ATAATGAAGA CAAGCCAGTA GGTGATAATG ATCAATCTTG CGATCAGGAA
    GTAAAATTGG AAATTCCTTC ATTCTGTAAA AGCAACAGCT TACCATTTAC CTGTAGCAGT
    GTAGAGAATG ATGGCTTATT ATCAGAATCT GACATTCAAA AAAATAATAA AATACCTGTT
    AGTCAGGATG ACCAGATGTG TCTAAAAGGA TCAATTCTCA TTAGAGAAAA TGCTCAGAAG
    ATAAATATAT GTGGAACCCC AGAAAAGTAT GACCAACCCA TGTCGTCCCT GCCAGAATCT
    GAACATCTAC AAATTGAGTC TTCTACAAGT AACAATTCTA GGATCTCTGA TGACTTTACC
    AATATGCTTT CTAAAAGTAA TACTGATAAT GAAAATCTTA ATAGTATTCC TGAACCAATG
    AGTAATGTAA CACAGCCACT TGTGGAGGTG ACTTTTAATC TGAACAATTT TGAAACCAGT
    GACTCTGAGG AGGAATCACA AGAAAATAAC AAAATTTCCC AGGATTCAGA GGGTTGGGTA
    AAGGAAACTC TGGTTAATGA TGGCAGTTCA AGTGTTCAAA AGAGATGTGA AAATATAAGC
    TGTAAGAAAG TTGGCAGTGA ACATTTGTCT CATTTAACAT CTATGGAGGA TAAGCCTACA
    GAGACATTTC CTAAAGAGAC TCTGCTGTCA CAGTTTTGTG ATAAAGCGTG TATGGGTTTT
    GACACAGGAC CTTGGAAAAC TGGAAACAGA AAAGAAATAG AAGAGTATAA TGACACATTA
    AGCAGTTTTG ATTCATCTTT AGAGTGGACT GATGATGTAT ATGTAGATAA TAATGAAGAT
    GCTAATAAAT CTATACAAAA AGTCAGAATT AACTATGATA TTGCTTCACT ACCGAGTAAG
    TCTAAAGGTA TAAGCTCAAA TTTACATATT CCTCAGTTAA ACATAGCCAC TAATCAGATT
    CCTAAAAACA GCCTATTCTC GGAACAGTCT GGGCTTAAGC CTTTTATAAT GGGAAATAAA
    TTAGACAAAA ATGATGAACA GGTTTTACTG TCAACTTCTA GCAGTGACAT CAGTATCCAA
    CAATTAAATG TTAATCAGAA TCACTCTGAA GAACCTGTTG CTCTTGGTAA ATCAAATACC
    CAAGTTTCCA ATTCTTTGTT GTACCTTCTC GGAGAAAAGC ATCCTATTTC CAAAGAAACA
    GAAGCGTGTA TTCCTGAATC CGAAGATTTG GGAAGGATTA GAAGTTTAGA CCATGACCAT
    ATTGAAGTAG AACCTGCTGG AGAAAGCAAA CAACATTGGA ATAGTCCTAG GAATCCTTCA
    GAACTCTCTG GATTAATAAA TAACATTTCC CTTTTAAAGT CATTGTCTGA ACATAGTACA
    GCTTTAGAAG GCTTGGAAAT ATTGAAAAAG AAAAATACTA CCTTTAAACA ACAAGGAACT
    CTACAGACAT ATAATCCAGA TAGTAATTCT GAAGCTAGGA AACGATCGAC TGCAGAAGTT
    TTGCAAGCTT TACCAAAGTT GCCTCCTCTG AACCAGGATT CTCAACAGAT TATAAAAGAA
    AATGAAGTTG AACCAAGTAA ACCACTTCAG TCTTTGCAGT TCTTTTCCTT TGGAGATAAA
    GAGACTGCTT TTCTAGATGT TATGCCTAAA CAATCAGAGA GAAAAACCTG TGACCCTAAG
    CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA GGATCTGCTA CTAGTGCTGT AATGATGAGA
    GGACACAGCT CACAGCTAGA ATGCGGTCAA TTTCCAGATA GTATTGAGTA TGAAAACTGC
    ACAAGAGACA CTTGTTTATT GACACCAAAA TTGACCAGTG CTTGTATGCA GATTGATTTC
    CTGCAGTTCC CAGATGAAAG AAACATTCCA AAAGAAGATA TTAGCTTTAA TATTGAAGAC
    AATTTTCAAG TGAGAGCTGA GTCAAATAAG GACTCAATTG AAAATGACTT GATTTTGGAT
    TTCTCTCCTA AAAATTCAGA ACATTTCTAT CATTATTTGG ATTCTAGTTC TAAATCAGCA
    GAGAAGACTT CATGGGAAGC AAATAAGGTA ACATCACCAG AACAGAAGAT CTCTACATTA
    AACCCTGATT CTACTTTTTC TTTGAACTCA CGAGATGAAG ACTTTGTGCT AGAATTCTCT
    GAGGAGTCCC TGAAAGAAAG AACTTTACCT GTTATCAAAA AATCCAGTTC TTTGGAGAAT
    AAGAACTTTC AAAGGCTTCC ATTACTCAGT TTTGCCAATC AGATCATTGT TAAGGAGATA
    AATGCCTCTG AGCTATGCTT CCCAAGTGGG CAGAAAATAA AATCTGCTTA TCTTCCCCAA
    AGGCAGATTC ATATACCAGC TGTTTTTCAG TCTCCTGCTC ATTATAAGCA GATTTTTACA
    TCTTGTCTCA TAGAACACTT AAATATATTG TTGTTTGGGT TGGCACAGAG GTTGCACAAA
    GCTCTTTCAA AAGTTGACAT ATCATTTTAC ACATCATTAA AGGGAGAGAA ACTAAAAAAT
    GTAGAAAGTA ATGTACCATC CTGCCATCAT CATCAACCTG CAAAACTTGT CATGGTTAAA
    AAGGAAGGTC CAAATAAGGG TCGTCTTTTT TATACCTGTG ATGGACCCAA AGCTGACCGA
    TGTAAATTTT TCAAGTGGCT TGAAGAAGTG ACCCCAGGAT ATTTGACACA AGAAGAGTCT
    CTACCTAGCA TAGTTTTAAG TGATATAAAG AGTATTGGCT TATACTTAAG AAGTCAAAAT
    ATACCCCTCT TTGAGGAATG TCAGCTTTTG GTGAGGAAAG GATTTGATTT TCAAAGAAAA
    CAGTATGGCA AACTGAAGAA GTTTAATGCT GTAAATCCTG AATTTTATAG TGAACCTAAA
    AGCAAACTTT ATCTTAAGCT GAGTCGGAGG GAAAGTTCTT CAACTTACAG CAAAAATGAT
    CTTTGGGTGG TTTCAAAAAC ATTGGACTTT GAATTGGATA CTTTTATTGC GTGTAGTGCT
    TTCTTTGGAC CATCATCTAT CAATGAGGTG GAGCTACTAC CTTTGAAAGG TTATTTCCCC
    TCTAACTGGC CCACTAACAT AGTGGTACAT GCATTGTTGG TTTGTAATGC TAGCACTGAG
    CTGACAACTT TGAAAAACAT TCAGGACTAC TTTAATCCAG CTACTCTACC TCTAACACAG
    TACTTGTTAA CAATGTCTTC ATCAACTATA GTTAGCAACA AGAGAGTCAA TAAGAGAAAA
    TTTATCCCAC CAGCCTTCTC AAATATCAAT ACAAAATTTG AACTTCTCAG CCTAGAAGCC
    ACATTACAGT TAGCTAGTGA ATTAATTCAG GCACACAACT TAAATAAGGA TCAAGCTACA
    GCTTTAATTC AGATAGCTCA AATGATGGCA TCACATGAAA ATGCTGAAGA AGTGAAGAAA
    GTACAAACCC ATACCCTCCC TATCACAATC ATACATGGTG TTTTTGGAGC AGGAAAGAGT
    TATTTGTTGG CAGTGGTGAT TTTGTTTTTT GTACAGCTAT TTGAAAAGAG TGAAGTTCTC
    ACAGTTGGAA ATGTAAGACC TTGGAAACTT CTCATTTCTT CTTCCACTAA TGTAGCTGTG
    GACAGAGTAC TTCTTGGGCT TCTCAGTCTT GGTTTTGAAA AGTTCGTCAG AGTTGGGAGT
    GTCAGGAAGA TTGCCAAGCC AATTTTACCT TATAGTTTGC ATGCTGGCTC AGAAAATGAA
    AATGAACAAC TAAAAGAACT ACATGGACTA ATGAAAGAAG ACTTGACTCC TCTGGAAAGG
    GTCTATGTCA GAAAAAGTAT TGAGCAGCAT AAACTGGGGA CCAATAAAAC CCTGCTGAAG
    CAGGTACGAG TAGTTGGAGT TACCTGTGCA GCTTGCCCAT TCCCATGTAT GAATGATCTT
    AAATTTCCTG TAGTTGTGTT GGATGAGTGT AGTCAAATAA CTGAACCGGC CTCTCTCCTT
    CCTATTGCAA GGTTTGAGTG TGAAAAGCTG ATTCTTGTTG GAGATCCTAA ACAGCTTTCT
    CCTACTATTC AGGGTTCTGA TGCAGCCCAT GAAAATGGAT TGGAACAAAC TCTTTTTGAT
    CGGCTTTGCT TAATGGGCCA TAAGCCAGTT CTGTTGAGAA CCCAGTACCG TTGTCACCCT
    GCAATCAGTG ATATCTCTAA TGATCTGTTT TATGAAGGAA ACCTTGTGGA TGGCATTTCT
    GAAACAGAGA GGGGCCCTTT ATTGGAATGG CTCCCAACAC TATGTTTCTA TAACATTAAA
    GGACCTGAAC AGATTGAAAG AGATAACAGC TTTCATAATG TGGCAGAAGC TGCTTTTACA
    CTCAAGCTGA TTCAATCACT GATTGCGAGT GGGATAGCGG GCTCTATGAT TGGGGTGATA
    ACATTATACA AATCCCAGAT GTACAAGCTT TGTCATTTAC TCAGTGCCGT GGACTTTGAC
    AATCCTGATA TTAAAGCTGT GCAGGTATCC ACAGTAGATG CTTTTCAGGG AGCTGAAAAG
    GAGATCATTA TTCTGTCCTG TGTAAGGACA AGACAAGTAG GATTTATTGA TTCAGAAAAA
    AGAATGAATG TTGCACTGAC CAGAGGAAGG AGGCATTTAT TGATCGTGGG AAATTTAGCC
    TGTTTGCGGA AAAATCGACT CTGGGGACGT GTAATCCAAC ACTGTGAAGG AAGGAGAGAT
    GGATTACAAC ATGCAAGCCA GTGTGAACCA CAGCTGAACC ATCTTCTTAA AGAGTATTTT
    GAAAAACGAG CAGAAGAAAA GCAGAAAAAA AGTGAAAAAG ATAAATCTAA AGATAAATCA
    CATTCACAAA AAGATATGGT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284017
    Clone ID Related Accession (Same CDS sequence) XM_022413418.1
    Accession Version XM_022413418.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5934bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein ZGRF1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGAAAGCC AGGAATTTAT TGTACTGTAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
    TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAGTTTT ATATGATGAC
    AAAGGAGAAT GTTTGGAGAG TATATTTCTT AAGTGCCTTG AGGTGAAGCC TGGAGATGAC
    TTAGAAGGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATTGCT
    ATTAAACCGG ATGTCATTAA AGAAACATCA GAGTTAAATC CAAGGAAATT TATATCCTCT
    GGGCGGTCCC TTGGATGTCA GCCTGCTGGT TTAAAAAGGA AGTTTACTGG TTTTCAAGGA
    CCACGTCAAG TGCCAAGGAA AATGGTTATT ACAGAAAATG ATGAATCAGC TGCATCACCT
    GAGGCTAAGA AACCTGGCCC TATTTTTCTT TCTCCCTTCT ACAACACACC TCCTTTGTTT
    TCTACTGCTG GCAAGAAAGA TACAAATAAT ATGCCAACAG ACCCTGAGAA CAGTGTCATT
    TACAAGAACA GAGAGAAAAA TGGCTTATAT TATTCTTCTG TTGTCTCTAC TCCATCCTTT
    AAGAATAACC CAGAAATGCT ATGTGAAGAA AATTATTTTT GCTCACCTGT CTATTCTGTA
    AATAAGCATT CAGATTCTTT ACTATCCAAT GAGGCCATAA AAAAAGATGG TTTGACATCT
    CACTGTTTAG GAGTTTCAGA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTAAAG
    TCCAAATCAA CTAGTATGGG TAAAGAACTA GATTCTGGGA TTACAGAACA TTTCCCTCAG
    ATACAACTAC AAGAAAGTGT AAACATTCCT TCTAAACCAA AGTGCATAAT TGAACAAGAC
    AAGTGTGGTG AGGTGAAGAG CACAGACAAT TTATACTACC AGCATCAATC GGAAAATACT
    ATGAGCTATA AAAGCAGATG GGCCATGTAT TTATCCTCAC AGAGTTCACC TGTACGTTCT
    TCTACTGAAG ATGGAAATAA TATAGAAAGG AAACCCAAAA CTCAGGGAGA TAATGTAAAT
    TTAAATTTGA AAGACCTTGT ACAAAAAAGG ATACAGTTTT TTGAAACATG TACTGAAAAG
    GGTAAAAACT ATAATGAAGA CAAGCCAGTA GGTGATAATG ATCAATCTTG CGATCAGGAA
    GTAAAATTGG AAATTCCTTC ATTCTGTAAA AGCAACAGCT TACCATTTAC CTGTAGCAGT
    GTAGAGAATG ATGGCTTATT ATCAGAATCT GACATTCAAA AAAATAATAA AATACCTGTT
    AGTCAGGATG ACCAGATGTG TCTAAAAGGA TCAATTCTCA TTAGAGAAAA TGCTCAGAAG
    ATAAATATAT GTGGAACCCC AGAAAAGTAT GACCAACCCA TGTCGTCCCT GCCAGAATCT
    GAACATCTAC AAATTGAGTC TTCTACAAGT AACAATTCTA GGATCTCTGA TGACTTTACC
    AATATGCTTT CTAAAAGTAA TACTGATAAT GAAAATCTTA ATAGTATTCC TGAACCAATG
    AGTAATGTAA CACAGCCACT TGTGGAGGTG ACTTTTAATC TGAACAATTT TGAAACCAGT
    GACTCTGAGG AGGAATCACA AGAAAATAAC AAAATTTCCC AGGATTCAGA GGGTTGGGTA
    AAGGAAACTC TGGTTAATGA TGGCAGTTCA AGTGTTCAAA AGAGATGTGA AAATATAAGC
    TGTAAGAAAG TTGGCAGTGA ACATTTGTCT CATTTAACAT CTATGGAGGA TAAGCCTACA
    GAGACATTTC CTAAAGAGAC TCTGCTGTCA CAGTTTTGTG ATAAAGCGTG TATGGGTTTT
    GACACAGGAC CTTGGAAAAC TGGAAACAGA AAAGAAATAG AAGAGTATAA TGACACATTA
    AGCAGTTTTG ATTCATCTTT AGAGTGGACT GATGATGTAT ATGTAGATAA TAATGAAGAT
    GCTAATAAAT CTATACAAAA AGTCAGAATT AACTATGATA TTGCTTCACT ACCGAGTAAG
    TCTAAAGGTA TAAGCTCAAA TTTACATATT CCTCAGTTAA ACATAGCCAC TAATCAGATT
    CCTAAAAACA GCCTATTCTC GGAACAGTCT GGGCTTAAGC CTTTTATAAT GGGAAATAAA
    TTAGACAAAA ATGATGAACA GGTTTTACTG TCAACTTCTA GCAGTGACAT CAGTATCCAA
    CAATTAAATG TTAATCAGAA TCACTCTGAA GAACCTGTTG CTCTTGGTAA ATCAAATACC
    CAAGTTTCCA ATTCTTTGTT GTACCTTCTC GGAGAAAAGC ATCCTATTTC CAAAGAAACA
    GAAGCGTGTA TTCCTGAATC CGAAGATTTG GGAAGGATTA GAAGTTTAGA CCATGACCAT
    ATTGAAGTAG AACCTGCTGG AGAAAGCAAA CAACATTGGA ATAGTCCTAG GAATCCTTCA
    GAACTCTCTG GATTAATAAA TAACATTTCC CTTTTAAAGT CATTGTCTGA ACATAGTACA
    GCTTTAGAAG GCTTGGAAAT ATTGAAAAAG AAAAATACTA CCTTTAAACA ACAAGGAACT
    CTACAGACAT ATAATCCAGA TAGTAATTCT GAAGCTAGGA AACGATCGAC TGCAGAAGTT
    TTGCAAGCTT TACCAAAGTT GCCTCCTCTG AACCAGGATT CTCAACAGAT TATAAAAGAA
    AATGAAGTTG AACCAAGTAA ACCACTTCAG TCTTTGCAGT TCTTTTCCTT TGGAGATAAA
    GAGACTGCTT TTCTAGATGT TATGCCTAAA CAATCAGAGA GAAAAACCTG TGACCCTAAG
    CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA GGATCTGCTA CTAGTGCTGT AATGATGAGA
    GGACACAGCT CACAGCTAGA ATGCGGTCAA TTTCCAGATA GTATTGAGTA TGAAAACTGC
    ACAAGAGACA CTTGTTTATT GACACCAAAA TTGACCAGTG CTTGTATGCA GATTGATTTC
    CTGCAGTTCC CAGATGAAAG AAACATTCCA AAAGAAGATA TTAGCTTTAA TATTGAAGAC
    AATTTTCAAG TGAGAGCTGA GTCAAATAAG GACTCAATTG AAAATGACTT GATTTTGGAT
    TTCTCTCCTA AAAATTCAGA ACATTTCTAT CATTATTTGG ATTCTAGTTC TAAATCAGCA
    GAGAAGACTT CATGGGAAGC AAATAAGGTA ACATCACCAG AACAGAAGAT CTCTACATTA
    AACCCTGATT CTACTTTTTC TTTGAACTCA CGAGATGAAG ACTTTGTGCT AGAATTCTCT
    GAGGAGTCCC TGAAAGAAAG AACTTTACCT GTTATCAAAA AATCCAGTTC TTTGGAGAAT
    AAGAACTTTC AAAGGCTTCC ATTAGAGATA AATGCCTCTG AGCTATGCTT CCCAAGTGGG
    CAGAAAATAA AATCTGCTTA TCTTCCCCAA AGGCAGATTC ATATACCAGC TGTTTTTCAG
    TCTCCTGCTC ATTATAAGCA GATTTTTACA TCTTGTCTCA TAGAACACTT AAATATATTG
    TTGTTTGGGT TGGCACAGAG GTTGCACAAA GCTCTTTCAA AAGTTGACAT ATCATTTTAC
    ACATCATTAA AGGGAGAGAA ACTAAAAAAT GTAGAAAGTA ATGTACCATC CTGCCATCAT
    CATCAACCTG CAAAACTTGT CATGGTTAAA AAGGAAGGTC CAAATAAGGG TCGTCTTTTT
    TATACCTGTG ATGGACCCAA AGCTGACCGA TGTAAATTTT TCAAGTGGCT TGAAGAAGTG
    ACCCCAGGAT ATTTGACACA AGAAGAGTCT CTACCTAGCA TAGTTTTAAG TGATATAAAG
    AGTATTGGCT TATACTTAAG AAGTCAAAAT ATACCCCTCT TTGAGGAATG TCAGCTTTTG
    GTGAGGAAAG GATTTGATTT TCAAAGAAAA CAGTATGGCA AACTGAAGAA GTTTAATGCT
    GTAAATCCTG AATTTTATAG TGAACCTAAA AGCAAACTTT ATCTTAAGCT GAGTCGGAGG
    GAAAGTTCTT CAACTTACAG CAAAAATGAT CTTTGGGTGG TTTCAAAAAC ATTGGACTTT
    GAATTGGATA CTTTTATTGC GTGTAGTGCT TTCTTTGGAC CATCATCTAT CAATGAGGTG
    GAGCTACTAC CTTTGAAAGG TTATTTCCCC TCTAACTGGC CCACTAACAT AGTGGTACAT
    GCATTGTTGG TTTGTAATGC TAGCACTGAG CTGACAACTT TGAAAAACAT TCAGGACTAC
    TTTAATCCAG CTACTCTACC TCTAACACAG TACTTGTTAA CAATGTCTTC ATCAACTATA
    GTTAGCAACA AGAGAGTCAA TAAGAGAAAA TTTATCCCAC CAGCCTTCTC AAATATCAAT
    ACAAAATTTG AACTTCTCAG CCTAGAAGCC ACATTACAGT TAGCTAGTGA ATTAATTCAG
    GCACACAACT TAAATAAGGA TCAAGCTACA GCTTTAATTC AGATAGCTCA AATGATGGCA
    TCACATGAAA ATGCTGAAGA AGTGAAGAAA GTACAAACCC ATACCCTCCC TATCACAATC
    ATACATGGTG TTTTTGGAGC AGGAAAGAGT TATTTGTTGG CAGTGGTGAT TTTGTTTTTT
    GTACAGCTAT TTGAAAAGAG TGAAGTTCTC ACAGTTGGAA ATGTAAGACC TTGGAAACTT
    CTCATTTCTT CTTCCACTAA TGTAGCTGTG GACAGAGTAC TTCTTGGGCT TCTCAGTCTT
    GGTTTTGAAA AGTTCGTCAG AGTTGGGAGT GTCAGGAAGA TTGCCAAGCC AATTTTACCT
    TATAGTTTGC ATGCTGGCTC AGAAAATGAA AATGAACAAC TAAAAGAACT ACATGGACTA
    ATGAAAGAAG ACTTGACTCC TCTGGAAAGG GTCTATGTCA GAAAAAGTAT TGAGCAGCAT
    AAACTGGGGA CCAATAAAAC CCTGCTGAAG CAGGTACGAG TAGTTGGAGT TACCTGTGCA
    GCTTGCCCAT TCCCATGTAT GAATGATCTT AAATTTCCTG TAGTTGTGTT GGATGAGTGT
    AGTCAAATAA CTGAACCGGC CTCTCTCCTT CCTATTGCAA GGTTTGAGTG TGAAAAGCTG
    ATTCTTGTTG GAGATCCTAA ACAGCTTTCT CCTACTATTC AGGGTTCTGA TGCAGCCCAT
    GAAAATGGAT TGGAACAAAC TCTTTTTGAT CGGCTTTGCT TAATGGGCCA TAAGCCAGTT
    CTGTTGAGAA CCCAGTACCG TTGTCACCCT GCAATCAGTG ATATCTCTAA TGATCTGTTT
    TATGAAGGAA ACCTTGTGGA TGGCATTTCT GAAACAGAGA GGGGCCCTTT ATTGGAATGG
    CTCCCAACAC TATGTTTCTA TAACATTAAA GGACCTGAAC AGATTGAAAG AGATAACAGC
    TTTCATAATG TGGCAGAAGC TGCTTTTACA CTCAAGCTGA TTCAATCACT GATTGCGAGT
    GGGATAGCGG GCTCTATGAT TGGGGTGATA ACATTATACA AATCCCAGAT GTACAAGCTT
    TGTCATTTAC TCAGTGCCGT GGACTTTGAC AATCCTGATA TTAAAGCTGT GCAGGTATCC
    ACAGTAGATG CTTTTCAGGG AGCTGAAAAG GAGATCATTA TTCTGTCCTG TGTAAGGACA
    AGACAAGTAG GATTTATTGA TTCAGAAAAA AGAATGAATG TTGCACTGAC CAGAGGAAGG
    AGGCATTTAT TGATCGTGGG AAATTTAGCC TGTTTGCGGA AAAATCGACT CTGGGGACGT
    GTAATCCAAC ACTGTGAAGG AAGGAGAGAT GGATTACAAC ATGCAAGCCA GTGTGAACCA
    CAGCTGAACC ATCTTCTTAA AGAGTATTTT GAAAAACGAG CAGAAGAAAA GCAGAAAAAA
    AGTGAAAAAG ATAAATCTAA AGATAAATCA CATTCACAAA AAGATATGGT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269126.1
    CDS314..6247
    Translation

    Target ORF information:

    RefSeq Version XM_022413418.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris zinc finger GRF-type containing 1 (ZGRF1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413418.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGAAAGCC AGGAATTTAT TGTACTGTAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
    TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAGTTTT ATATGATGAC
    AAAGGAGAAT GTTTGGAGAG TATATTTCTT AAGTGCCTTG AGGTGAAGCC TGGAGATGAC
    TTAGAAGGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATTGCT
    ATTAAACCGG ATGTCATTAA AGAAACATCA GAGTTAAATC CAAGGAAATT TATATCCTCT
    GGGCGGTCCC TTGGATGTCA GCCTGCTGGT TTAAAAAGGA AGTTTACTGG TTTTCAAGGA
    CCACGTCAAG TGCCAAGGAA AATGGTTATT ACAGAAAATG ATGAATCAGC TGCATCACCT
    GAGGCTAAGA AACCTGGCCC TATTTTTCTT TCTCCCTTCT ACAACACACC TCCTTTGTTT
    TCTACTGCTG GCAAGAAAGA TACAAATAAT ATGCCAACAG ACCCTGAGAA CAGTGTCATT
    TACAAGAACA GAGAGAAAAA TGGCTTATAT TATTCTTCTG TTGTCTCTAC TCCATCCTTT
    AAGAATAACC CAGAAATGCT ATGTGAAGAA AATTATTTTT GCTCACCTGT CTATTCTGTA
    AATAAGCATT CAGATTCTTT ACTATCCAAT GAGGCCATAA AAAAAGATGG TTTGACATCT
    CACTGTTTAG GAGTTTCAGA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTAAAG
    TCCAAATCAA CTAGTATGGG TAAAGAACTA GATTCTGGGA TTACAGAACA TTTCCCTCAG
    ATACAACTAC AAGAAAGTGT AAACATTCCT TCTAAACCAA AGTGCATAAT TGAACAAGAC
    AAGTGTGGTG AGGTGAAGAG CACAGACAAT TTATACTACC AGCATCAATC GGAAAATACT
    ATGAGCTATA AAAGCAGATG GGCCATGTAT TTATCCTCAC AGAGTTCACC TGTACGTTCT
    TCTACTGAAG ATGGAAATAA TATAGAAAGG AAACCCAAAA CTCAGGGAGA TAATGTAAAT
    TTAAATTTGA AAGACCTTGT ACAAAAAAGG ATACAGTTTT TTGAAACATG TACTGAAAAG
    GGTAAAAACT ATAATGAAGA CAAGCCAGTA GGTGATAATG ATCAATCTTG CGATCAGGAA
    GTAAAATTGG AAATTCCTTC ATTCTGTAAA AGCAACAGCT TACCATTTAC CTGTAGCAGT
    GTAGAGAATG ATGGCTTATT ATCAGAATCT GACATTCAAA AAAATAATAA AATACCTGTT
    AGTCAGGATG ACCAGATGTG TCTAAAAGGA TCAATTCTCA TTAGAGAAAA TGCTCAGAAG
    ATAAATATAT GTGGAACCCC AGAAAAGTAT GACCAACCCA TGTCGTCCCT GCCAGAATCT
    GAACATCTAC AAATTGAGTC TTCTACAAGT AACAATTCTA GGATCTCTGA TGACTTTACC
    AATATGCTTT CTAAAAGTAA TACTGATAAT GAAAATCTTA ATAGTATTCC TGAACCAATG
    AGTAATGTAA CACAGCCACT TGTGGAGGTG ACTTTTAATC TGAACAATTT TGAAACCAGT
    GACTCTGAGG AGGAATCACA AGAAAATAAC AAAATTTCCC AGGATTCAGA GGGTTGGGTA
    AAGGAAACTC TGGTTAATGA TGGCAGTTCA AGTGTTCAAA AGAGATGTGA AAATATAAGC
    TGTAAGAAAG TTGGCAGTGA ACATTTGTCT CATTTAACAT CTATGGAGGA TAAGCCTACA
    GAGACATTTC CTAAAGAGAC TCTGCTGTCA CAGTTTTGTG ATAAAGCGTG TATGGGTTTT
    GACACAGGAC CTTGGAAAAC TGGAAACAGA AAAGAAATAG AAGAGTATAA TGACACATTA
    AGCAGTTTTG ATTCATCTTT AGAGTGGACT GATGATGTAT ATGTAGATAA TAATGAAGAT
    GCTAATAAAT CTATACAAAA AGTCAGAATT AACTATGATA TTGCTTCACT ACCGAGTAAG
    TCTAAAGGTA TAAGCTCAAA TTTACATATT CCTCAGTTAA ACATAGCCAC TAATCAGATT
    CCTAAAAACA GCCTATTCTC GGAACAGTCT GGGCTTAAGC CTTTTATAAT GGGAAATAAA
    TTAGACAAAA ATGATGAACA GGTTTTACTG TCAACTTCTA GCAGTGACAT CAGTATCCAA
    CAATTAAATG TTAATCAGAA TCACTCTGAA GAACCTGTTG CTCTTGGTAA ATCAAATACC
    CAAGTTTCCA ATTCTTTGTT GTACCTTCTC GGAGAAAAGC ATCCTATTTC CAAAGAAACA
    GAAGCGTGTA TTCCTGAATC CGAAGATTTG GGAAGGATTA GAAGTTTAGA CCATGACCAT
    ATTGAAGTAG AACCTGCTGG AGAAAGCAAA CAACATTGGA ATAGTCCTAG GAATCCTTCA
    GAACTCTCTG GATTAATAAA TAACATTTCC CTTTTAAAGT CATTGTCTGA ACATAGTACA
    GCTTTAGAAG GCTTGGAAAT ATTGAAAAAG AAAAATACTA CCTTTAAACA ACAAGGAACT
    CTACAGACAT ATAATCCAGA TAGTAATTCT GAAGCTAGGA AACGATCGAC TGCAGAAGTT
    TTGCAAGCTT TACCAAAGTT GCCTCCTCTG AACCAGGATT CTCAACAGAT TATAAAAGAA
    AATGAAGTTG AACCAAGTAA ACCACTTCAG TCTTTGCAGT TCTTTTCCTT TGGAGATAAA
    GAGACTGCTT TTCTAGATGT TATGCCTAAA CAATCAGAGA GAAAAACCTG TGACCCTAAG
    CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA GGATCTGCTA CTAGTGCTGT AATGATGAGA
    GGACACAGCT CACAGCTAGA ATGCGGTCAA TTTCCAGATA GTATTGAGTA TGAAAACTGC
    ACAAGAGACA CTTGTTTATT GACACCAAAA TTGACCAGTG CTTGTATGCA GATTGATTTC
    CTGCAGTTCC CAGATGAAAG AAACATTCCA AAAGAAGATA TTAGCTTTAA TATTGAAGAC
    AATTTTCAAG TGAGAGCTGA GTCAAATAAG GACTCAATTG AAAATGACTT GATTTTGGAT
    TTCTCTCCTA AAAATTCAGA ACATTTCTAT CATTATTTGG ATTCTAGTTC TAAATCAGCA
    GAGAAGACTT CATGGGAAGC AAATAAGGTA ACATCACCAG AACAGAAGAT CTCTACATTA
    AACCCTGATT CTACTTTTTC TTTGAACTCA CGAGATGAAG ACTTTGTGCT AGAATTCTCT
    GAGGAGTCCC TGAAAGAAAG AACTTTACCT GTTATCAAAA AATCCAGTTC TTTGGAGAAT
    AAGAACTTTC AAAGGCTTCC ATTAGAGATA AATGCCTCTG AGCTATGCTT CCCAAGTGGG
    CAGAAAATAA AATCTGCTTA TCTTCCCCAA AGGCAGATTC ATATACCAGC TGTTTTTCAG
    TCTCCTGCTC ATTATAAGCA GATTTTTACA TCTTGTCTCA TAGAACACTT AAATATATTG
    TTGTTTGGGT TGGCACAGAG GTTGCACAAA GCTCTTTCAA AAGTTGACAT ATCATTTTAC
    ACATCATTAA AGGGAGAGAA ACTAAAAAAT GTAGAAAGTA ATGTACCATC CTGCCATCAT
    CATCAACCTG CAAAACTTGT CATGGTTAAA AAGGAAGGTC CAAATAAGGG TCGTCTTTTT
    TATACCTGTG ATGGACCCAA AGCTGACCGA TGTAAATTTT TCAAGTGGCT TGAAGAAGTG
    ACCCCAGGAT ATTTGACACA AGAAGAGTCT CTACCTAGCA TAGTTTTAAG TGATATAAAG
    AGTATTGGCT TATACTTAAG AAGTCAAAAT ATACCCCTCT TTGAGGAATG TCAGCTTTTG
    GTGAGGAAAG GATTTGATTT TCAAAGAAAA CAGTATGGCA AACTGAAGAA GTTTAATGCT
    GTAAATCCTG AATTTTATAG TGAACCTAAA AGCAAACTTT ATCTTAAGCT GAGTCGGAGG
    GAAAGTTCTT CAACTTACAG CAAAAATGAT CTTTGGGTGG TTTCAAAAAC ATTGGACTTT
    GAATTGGATA CTTTTATTGC GTGTAGTGCT TTCTTTGGAC CATCATCTAT CAATGAGGTG
    GAGCTACTAC CTTTGAAAGG TTATTTCCCC TCTAACTGGC CCACTAACAT AGTGGTACAT
    GCATTGTTGG TTTGTAATGC TAGCACTGAG CTGACAACTT TGAAAAACAT TCAGGACTAC
    TTTAATCCAG CTACTCTACC TCTAACACAG TACTTGTTAA CAATGTCTTC ATCAACTATA
    GTTAGCAACA AGAGAGTCAA TAAGAGAAAA TTTATCCCAC CAGCCTTCTC AAATATCAAT
    ACAAAATTTG AACTTCTCAG CCTAGAAGCC ACATTACAGT TAGCTAGTGA ATTAATTCAG
    GCACACAACT TAAATAAGGA TCAAGCTACA GCTTTAATTC AGATAGCTCA AATGATGGCA
    TCACATGAAA ATGCTGAAGA AGTGAAGAAA GTACAAACCC ATACCCTCCC TATCACAATC
    ATACATGGTG TTTTTGGAGC AGGAAAGAGT TATTTGTTGG CAGTGGTGAT TTTGTTTTTT
    GTACAGCTAT TTGAAAAGAG TGAAGTTCTC ACAGTTGGAA ATGTAAGACC TTGGAAACTT
    CTCATTTCTT CTTCCACTAA TGTAGCTGTG GACAGAGTAC TTCTTGGGCT TCTCAGTCTT
    GGTTTTGAAA AGTTCGTCAG AGTTGGGAGT GTCAGGAAGA TTGCCAAGCC AATTTTACCT
    TATAGTTTGC ATGCTGGCTC AGAAAATGAA AATGAACAAC TAAAAGAACT ACATGGACTA
    ATGAAAGAAG ACTTGACTCC TCTGGAAAGG GTCTATGTCA GAAAAAGTAT TGAGCAGCAT
    AAACTGGGGA CCAATAAAAC CCTGCTGAAG CAGGTACGAG TAGTTGGAGT TACCTGTGCA
    GCTTGCCCAT TCCCATGTAT GAATGATCTT AAATTTCCTG TAGTTGTGTT GGATGAGTGT
    AGTCAAATAA CTGAACCGGC CTCTCTCCTT CCTATTGCAA GGTTTGAGTG TGAAAAGCTG
    ATTCTTGTTG GAGATCCTAA ACAGCTTTCT CCTACTATTC AGGGTTCTGA TGCAGCCCAT
    GAAAATGGAT TGGAACAAAC TCTTTTTGAT CGGCTTTGCT TAATGGGCCA TAAGCCAGTT
    CTGTTGAGAA CCCAGTACCG TTGTCACCCT GCAATCAGTG ATATCTCTAA TGATCTGTTT
    TATGAAGGAA ACCTTGTGGA TGGCATTTCT GAAACAGAGA GGGGCCCTTT ATTGGAATGG
    CTCCCAACAC TATGTTTCTA TAACATTAAA GGACCTGAAC AGATTGAAAG AGATAACAGC
    TTTCATAATG TGGCAGAAGC TGCTTTTACA CTCAAGCTGA TTCAATCACT GATTGCGAGT
    GGGATAGCGG GCTCTATGAT TGGGGTGATA ACATTATACA AATCCCAGAT GTACAAGCTT
    TGTCATTTAC TCAGTGCCGT GGACTTTGAC AATCCTGATA TTAAAGCTGT GCAGGTATCC
    ACAGTAGATG CTTTTCAGGG AGCTGAAAAG GAGATCATTA TTCTGTCCTG TGTAAGGACA
    AGACAAGTAG GATTTATTGA TTCAGAAAAA AGAATGAATG TTGCACTGAC CAGAGGAAGG
    AGGCATTTAT TGATCGTGGG AAATTTAGCC TGTTTGCGGA AAAATCGACT CTGGGGACGT
    GTAATCCAAC ACTGTGAAGG AAGGAGAGAT GGATTACAAC ATGCAAGCCA GTGTGAACCA
    CAGCTGAACC ATCTTCTTAA AGAGTATTTT GAAAAACGAG CAGAAGAAAA GCAGAAAAAA
    AGTGAAAAAG ATAAATCTAA AGATAAATCA CATTCACAAA AAGATATGGT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284018
    Clone ID Related Accession (Same CDS sequence) XM_022413419.1
    Accession Version XM_022413419.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5739bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein ZGRF1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGAAAGCC AGGAATTTAT TGTACTGTAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
    TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAGTTTT ATATGATGAC
    AAAGGAGAAT GTTTGGAGAG TATATTTCTT AAGTGCCTTG AGGTGAAGCC TGGAGATGAC
    TTAGAAGGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATTGCT
    ATTAAACCGG ATGTCATTAA AGAAACATCA GAGTTAAATC CAAGGAAATT TATATCCTCT
    GGGCGGTCCC TTGGATGTCA GCCTGCTGGT TTAAAAAGGA AGTTTACTGG TTTTCAAGGA
    CCACGTCAAG TGCCAAGGAA AATGGTTATT ACAGAAAATG ATGAATCAGC TGCATCACCT
    GAGGCTAAGA AACCTGGCCC TATTTTTCTT TCTCCCTTCT ACAACACACC TCCTTTGTTT
    TCTACTGCTG GCAAGAAAGA TACAAATAAT ATGCCAACAG ACCCTGAGAA CAGTGTCATT
    TACAAGAACA GAGAGAAAAA TGGCTTATAT TATTCTTCTG TTGTCTCTAC TCCATCCTTT
    AAGAATAACC CAGAAATGCT ATGTGAAGAA AATTATTTTT GCTCACCTGT CTATTCTGTA
    AATAAGCATT CAGATTCTTT ACTATCCAAT GAGGCCATAA AAAAAGATGG TTTGACATCT
    CACTGTTTAG GAGTTTCAGA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTAAAG
    TCCAAATCAA CTAGTATGGG TAAAGAACTA GATTCTGGGA TTACAGAACA TTTCCCTCAG
    ATACAACTAC AAGAAAGTGT AAACATTCCT TCTAAACCAA AGTGCATAAT TGAACAAGAC
    AAGTGTGGTG AGGTGAAGAG CACAGACAAT TTATACTACC AGCATCAATC GGAAAATACT
    ATGAGCTATA AAAGCAGATG GGCCATGTAT TTATCCTCAC AGAGTTCACC TGTACGTTCT
    TCTACTGAAG ATGGAAATAA TATAGAAAGG AAACCCAAAA CTCAGGGAGA TAATGTAAAT
    TTAAATTTGA AAGACCTTGT ACAAAAAAGG ATACAGTTTT TTGAAACATG TACTGAAAAG
    GGTAAAAACT ATAATGAAGA CAAGCCAGTA GGTGATAATG ATCAATCTTG CGATCAGGAA
    GTAAAATTGG AAATTCCTTC ATTCTGTAAA AGCAACAGCT TACCATTTAC CTGTAGCAGT
    GTAGAGAATG ATGGCTTATT ATCAGAATCT GACATTCAAA AAAATAATAA AATACCTGTT
    AGTCAGGATG ACCAGATGTG TCTAAAAGGA TCAATTCTCA TTAGAGAAAA TGCTCAGAAG
    ATAAATATAT GTGGAACCCC AGAAAAGTAT GACCAACCCA TGTCGTCCCT GCCAGAATCT
    GAACATCTAC AAATTGAGTC TTCTACAAGT AACAATTCTA GGATCTCTGA TGACTTTACC
    AATATGCTTT CTAAAAGTAA TACTGATAAT GAAAATCTTA ATAGTATTCC TGAACCAATG
    AGTAATGTAA CACAGCCACT TGTGGAGGTG ACTTTTAATC TGAACAATTT TGAAACCAGT
    GACTCTGAGG AGGAATCACA AGAAAATAAC AAAATTTCCC AGGATTCAGA GGGTTGGGTA
    AAGGAAACTC TGGTTAATGA TGGCAGTTCA AGTGTTCAAA AGAGATGTGA AAATATAAGC
    TGTAAGAAAG TTGGCAGTGA ACATTTGTCT CATTTAACAT CTATGGAGGA TAAGCCTACA
    GAGACATTTC CTAAAGAGAC TCTGCTGTCA CAGTTTTGTG ATAAAGCGTG TATGGGTTTT
    GACACAGGAC CTTGGAAAAC TGGAAACAGA AAAGAAATAG AAGAGTATAA TGACACATTA
    AGCAGTTTTG ATTCATCTTT AGAGTGGACT GATGATGTAT ATGTAGATAA TAATGAAGAT
    GCTAATAAAT CTATACAAAA AGTCAGAATT AACTATGATA TTGCTTCACT ACCGAGTAAG
    TCTAAAGGTA TAAGCTCAAA TTTACATATT CCTCAGTTAA ACATAGCCAC TAATCAGATT
    CCTAAAAACA GCCTATTCTC GGAACAGTCT GGGCTTAAGC CTTTTATAAT GGGAAATAAA
    TTAGACAAAA ATGATGAACA GGTTTTACTG TCAACTTCTA GCAGTGACAT CAGTATCCAA
    CAATTAAATG TTAATCAGAA TCACTCTGAA GAACCTGTTG CTCTTGGTAA ATCAAATACC
    CAAGTTTCCA ATTCTTTGTT GTACCTTCTC GGAGAAAAGC ATCCTATTTC CAAAGAAACA
    GAAGCGTGTA TTCCTGAATC CGAAGATTTG GGAAGGATTA GAAGTTTAGA CCATGACCAT
    ATTGAAGTAG AACCTGCTGG AGAAAGCAAA CAACATTGGA ATAGTCCTAG GAATCCTTCA
    GAACTCTCTG GATTAATAAA TAACATTTCC CTTTTAAAGT CATTGTCTGA ACATAGTACA
    GCTTTAGAAG GCTTGGAAAT ATTGAAAAAG AAAAATACTA CCTTTAAACA ACAAGGAACT
    CTACAGACAT ATAATCCAGA TAGTAATTCT GAAGCTAGGA AACGATCGAC TGCAGAAGTT
    TTGCAAGCTT TACCAAAGTT GCCTCCTCTG AACCAGGATT CTCAACAGAT TATAAAAGAA
    AATGAAGTTG AACCAAGTAA ACCACTTCAG TCTTTGCAGT TCTTTTCCTT TGGAGATAAA
    GAGACTGCTT TTCTAGATGT TATGCCTAAA CAATCAGAGA GAAAAACCTG TGACCCTAAG
    CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA GGATCTGCTA CTAGTGCTGT AATGATGAGA
    GGACACAGCT CACAGCTAGA ATGCGGTCAA TTTCCAGATA GTATTGAGTA TGAAAACTGC
    ACAAGAGACA CTTGTTTATT GACACCAAAA TTGACCAGTG CTTGTATGCA GATTGATTTC
    CTGCAGTTCC CAGATGAAAG AAACATTCCA AAAGAAGATA TTAGCTTTAA TATTGAAGAC
    AATTTTCAAG TGAGAGCTGA GTCAAATAAG GACTCAATTG AAAATGACTT GATTTTGGAT
    TTCTCTCCTA AAAATTCAGA ACATTTCTAT CATTATTTGG ATTCTAGTTC TAAATCAGCA
    GAGAAGACTT CATGGGAAGC AAATAAGGTA ACATCACCAG AACAGAAGAT CTCTACATTA
    AACCCTGATT CTACTTTTTC TTTGAACTCA CGAGATGAAG ACTTTGTGCT AGAATTCTCT
    GAGGAGTCCC TGAAAGAAAG AACTTTACCT GTTATCAAAA AATCCAGTTC TTTGGAGAAT
    AAGAACTTTC AAAGGCTTCC ATTAGTAAGT AGAACCCGAG TTCCACTAAC TACTTTGCCA
    CCTGCTGATT CAGATTCTTG TTCATACGTG ATCAACTGTG ACAGGCAAGA ATATTCTGGT
    TCCTCCCCCA TATTCAACTT ATGTGAAGAA TCAGGAGTTC TTTCATTTAG CTTGGGGCTT
    GAGGACCAAT GTGAAACTTC CTTCTGTAAA GAATCTAGAG AAGTGATTCC ACAGGACGTT
    TTGCTTCTCA ATAATATACC TACTCAAAGC AAGTGGCTGA AATATCAGAA CATACCCCAG
    TGCAACTTGA CTACTCCAGA CAGAGTTGAT AAGAAAGTAA CAGATGGTTT CTCTGGTAAA
    GTTATTTCTG GGACACATTT AAGTGATATA CGTGAAAGAC AGAGTGGTGC TGTGAATGAA
    AGCTCTTTAG ACTCTATATA TTTGCAAATG ATAAAAGGCA TGCTCTATCA ACAGCAGCAG
    GATTTTAGCT GTCAGGATTT GGTTTTCAGA AAGAAAGCAC TCTCATTGAA TTTAAAGCTG
    ACTTCCCAGA CTGAAAAAAT TCAAAACACG TTAAGAGAGT CTGCTGCCTG CTACATCTAC
    AGTGTAACAG GAGATTTACA GGAGATAAAT GCCTCTGAGC TATGCTTCCC AAGTGGGCAG
    AAAATAAAAT CTGCTTATCT TCCCCAAAGG CAGATTCATA TACCAGCTGT TTTTCAGTCT
    CCTGCTCATT ATAAGCAGAT TTTTACATCT TGTCTCATAG AACACTTAAA TATATTGTTG
    TTTGGGTTGG CACAGAGGTT GCACAAAGCT CTTTCAAAAG TTGACATATC ATTTTACACA
    TCATTAAAGG GAGAGAAACT AAAAAATGTA GAAAGTAATG TACCATCCTG CCATCATCAT
    CAACCTGCAA AACTTGTCAT GGTTAAAAAG GAAGGTCCAA ATAAGGGTCG TCTTTTTTAT
    ACCTGTGATG GACCCAAAGC TGACCGATGT AAATTTTTCA AGTGGCTTGA AGAAGTGACC
    CCAGGATATT TGACACAAGA AGAGTCTCTA CCTAGCATAG TTTTAAGTGA TATAAAGAGT
    ATTGGCTTAT ACTTAAGAAG TCAAAATATA CCCCTCTTTG AGGAATGTCA GCTTTTGGTG
    AGGAAAGGAT TTGATTTTCA AAGAAAACAG TATGGCAAAC TGAAGAAGTT TAATGCTGTA
    AATCCTGAAT TTTATAGTGA ACCTAAAAGC AAACTTTATC TTAAGCTGAG TCGGAGGGAA
    AGTTCTTCAA CTTACAGCAA AAATGATCTT TGGGTGGTTT CAAAAACATT GGACTTTGAA
    TTGGATACTT TTATTGCGTG TAGTGCTTTC TTTGGACCAT CATCTATCAA TGAGGTGGAG
    CTACTACCTT TGAAAGGTTA TTTCCCCTCT AACTGGCCCA CTAACATAGT GGTACATGCA
    TTGTTGGTTT GTAATGCTAG CACTGAGCTG ACAACTTTGA AAAACATTCA GGACTACTTT
    AATCCAGCTA CTCTACCTCT AACACAGTAC TTGTTAACAA TGTCTTCATC AACTATAGTT
    AGCAACAAGA GAGTCAATAA GAGAAAATTT ATCCCACCAG CCTTCTCAAA TATCAATACA
    AAATTTGAAC TTCTCAGCCT AGAAGCCACA TTACAGTTAG CTAGTGAATT AATTCAGGCA
    CACAACTTAA ATAAGGATCA AGCTACAGCT TTAATTCAGA TAGCTCAAAT GATGGCATCA
    CATGAAAATG CTGAAGAAGT GAAGAAAGTA CAAACCCATA CCCTCCCTAT CACAATCATA
    CATGGTGTTT TTGGAGCAGG AAAGAGTTAT TTGTTGGCAG TGGTGATTTT GTTTTTTGTA
    CAGCTATTTG AAAAGAGTGA AGTTCTCACA GTTGGAAATG TAAGACCTTG GAAACTTCTC
    ATTTCTTCTT CCACTAATGT AGCTGTGGAC AGAGTACTTC TTGGGCTTCT CAGTCTTGGT
    TTTGAAAAGT TCGTCAGAGT TGGGAGTGTC AGGAAGATTG CCAAGCCAAT TTTACCTTAT
    AGTTTGCATG CTGGCTCAGA AAATGAAAAT GAACAACTAA AAGAACTACA TGGACTAATG
    AAAGAAGACT TGACTCCTCT GGAAAGGGTC TATGTCAGAA AAAGTATTGA GCAGCATAAA
    CTGGGGACCA ATAAAACCCT GCTGAAGCAG GTACGAGTAG TTGGAGTTAC CTGTGCAGCT
    TGCCCATTCC CATGTATGAA TGATCTTAAA TTTCCTGTAG TTGTGTTGGA TGAGTGTAGT
    CAAATAACTG AACCGGCCTC TCTCCTTCCT ATTGCAAGGG TTCTGATGCA GCCCATGAAA
    ATGGATTGGA ACAAACTCTT TTTGATCGGC TTTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269127.1
    CDS321..6059
    Translation

    Target ORF information:

    RefSeq Version XM_022413419.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris zinc finger GRF-type containing 1 (ZGRF1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413419.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGAAAGCC AGGAATTTAT TGTACTGTAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
    TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAGTTTT ATATGATGAC
    AAAGGAGAAT GTTTGGAGAG TATATTTCTT AAGTGCCTTG AGGTGAAGCC TGGAGATGAC
    TTAGAAGGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATTGCT
    ATTAAACCGG ATGTCATTAA AGAAACATCA GAGTTAAATC CAAGGAAATT TATATCCTCT
    GGGCGGTCCC TTGGATGTCA GCCTGCTGGT TTAAAAAGGA AGTTTACTGG TTTTCAAGGA
    CCACGTCAAG TGCCAAGGAA AATGGTTATT ACAGAAAATG ATGAATCAGC TGCATCACCT
    GAGGCTAAGA AACCTGGCCC TATTTTTCTT TCTCCCTTCT ACAACACACC TCCTTTGTTT
    TCTACTGCTG GCAAGAAAGA TACAAATAAT ATGCCAACAG ACCCTGAGAA CAGTGTCATT
    TACAAGAACA GAGAGAAAAA TGGCTTATAT TATTCTTCTG TTGTCTCTAC TCCATCCTTT
    AAGAATAACC CAGAAATGCT ATGTGAAGAA AATTATTTTT GCTCACCTGT CTATTCTGTA
    AATAAGCATT CAGATTCTTT ACTATCCAAT GAGGCCATAA AAAAAGATGG TTTGACATCT
    CACTGTTTAG GAGTTTCAGA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTAAAG
    TCCAAATCAA CTAGTATGGG TAAAGAACTA GATTCTGGGA TTACAGAACA TTTCCCTCAG
    ATACAACTAC AAGAAAGTGT AAACATTCCT TCTAAACCAA AGTGCATAAT TGAACAAGAC
    AAGTGTGGTG AGGTGAAGAG CACAGACAAT TTATACTACC AGCATCAATC GGAAAATACT
    ATGAGCTATA AAAGCAGATG GGCCATGTAT TTATCCTCAC AGAGTTCACC TGTACGTTCT
    TCTACTGAAG ATGGAAATAA TATAGAAAGG AAACCCAAAA CTCAGGGAGA TAATGTAAAT
    TTAAATTTGA AAGACCTTGT ACAAAAAAGG ATACAGTTTT TTGAAACATG TACTGAAAAG
    GGTAAAAACT ATAATGAAGA CAAGCCAGTA GGTGATAATG ATCAATCTTG CGATCAGGAA
    GTAAAATTGG AAATTCCTTC ATTCTGTAAA AGCAACAGCT TACCATTTAC CTGTAGCAGT
    GTAGAGAATG ATGGCTTATT ATCAGAATCT GACATTCAAA AAAATAATAA AATACCTGTT
    AGTCAGGATG ACCAGATGTG TCTAAAAGGA TCAATTCTCA TTAGAGAAAA TGCTCAGAAG
    ATAAATATAT GTGGAACCCC AGAAAAGTAT GACCAACCCA TGTCGTCCCT GCCAGAATCT
    GAACATCTAC AAATTGAGTC TTCTACAAGT AACAATTCTA GGATCTCTGA TGACTTTACC
    AATATGCTTT CTAAAAGTAA TACTGATAAT GAAAATCTTA ATAGTATTCC TGAACCAATG
    AGTAATGTAA CACAGCCACT TGTGGAGGTG ACTTTTAATC TGAACAATTT TGAAACCAGT
    GACTCTGAGG AGGAATCACA AGAAAATAAC AAAATTTCCC AGGATTCAGA GGGTTGGGTA
    AAGGAAACTC TGGTTAATGA TGGCAGTTCA AGTGTTCAAA AGAGATGTGA AAATATAAGC
    TGTAAGAAAG TTGGCAGTGA ACATTTGTCT CATTTAACAT CTATGGAGGA TAAGCCTACA
    GAGACATTTC CTAAAGAGAC TCTGCTGTCA CAGTTTTGTG ATAAAGCGTG TATGGGTTTT
    GACACAGGAC CTTGGAAAAC TGGAAACAGA AAAGAAATAG AAGAGTATAA TGACACATTA
    AGCAGTTTTG ATTCATCTTT AGAGTGGACT GATGATGTAT ATGTAGATAA TAATGAAGAT
    GCTAATAAAT CTATACAAAA AGTCAGAATT AACTATGATA TTGCTTCACT ACCGAGTAAG
    TCTAAAGGTA TAAGCTCAAA TTTACATATT CCTCAGTTAA ACATAGCCAC TAATCAGATT
    CCTAAAAACA GCCTATTCTC GGAACAGTCT GGGCTTAAGC CTTTTATAAT GGGAAATAAA
    TTAGACAAAA ATGATGAACA GGTTTTACTG TCAACTTCTA GCAGTGACAT CAGTATCCAA
    CAATTAAATG TTAATCAGAA TCACTCTGAA GAACCTGTTG CTCTTGGTAA ATCAAATACC
    CAAGTTTCCA ATTCTTTGTT GTACCTTCTC GGAGAAAAGC ATCCTATTTC CAAAGAAACA
    GAAGCGTGTA TTCCTGAATC CGAAGATTTG GGAAGGATTA GAAGTTTAGA CCATGACCAT
    ATTGAAGTAG AACCTGCTGG AGAAAGCAAA CAACATTGGA ATAGTCCTAG GAATCCTTCA
    GAACTCTCTG GATTAATAAA TAACATTTCC CTTTTAAAGT CATTGTCTGA ACATAGTACA
    GCTTTAGAAG GCTTGGAAAT ATTGAAAAAG AAAAATACTA CCTTTAAACA ACAAGGAACT
    CTACAGACAT ATAATCCAGA TAGTAATTCT GAAGCTAGGA AACGATCGAC TGCAGAAGTT
    TTGCAAGCTT TACCAAAGTT GCCTCCTCTG AACCAGGATT CTCAACAGAT TATAAAAGAA
    AATGAAGTTG AACCAAGTAA ACCACTTCAG TCTTTGCAGT TCTTTTCCTT TGGAGATAAA
    GAGACTGCTT TTCTAGATGT TATGCCTAAA CAATCAGAGA GAAAAACCTG TGACCCTAAG
    CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA GGATCTGCTA CTAGTGCTGT AATGATGAGA
    GGACACAGCT CACAGCTAGA ATGCGGTCAA TTTCCAGATA GTATTGAGTA TGAAAACTGC
    ACAAGAGACA CTTGTTTATT GACACCAAAA TTGACCAGTG CTTGTATGCA GATTGATTTC
    CTGCAGTTCC CAGATGAAAG AAACATTCCA AAAGAAGATA TTAGCTTTAA TATTGAAGAC
    AATTTTCAAG TGAGAGCTGA GTCAAATAAG GACTCAATTG AAAATGACTT GATTTTGGAT
    TTCTCTCCTA AAAATTCAGA ACATTTCTAT CATTATTTGG ATTCTAGTTC TAAATCAGCA
    GAGAAGACTT CATGGGAAGC AAATAAGGTA ACATCACCAG AACAGAAGAT CTCTACATTA
    AACCCTGATT CTACTTTTTC TTTGAACTCA CGAGATGAAG ACTTTGTGCT AGAATTCTCT
    GAGGAGTCCC TGAAAGAAAG AACTTTACCT GTTATCAAAA AATCCAGTTC TTTGGAGAAT
    AAGAACTTTC AAAGGCTTCC ATTAGTAAGT AGAACCCGAG TTCCACTAAC TACTTTGCCA
    CCTGCTGATT CAGATTCTTG TTCATACGTG ATCAACTGTG ACAGGCAAGA ATATTCTGGT
    TCCTCCCCCA TATTCAACTT ATGTGAAGAA TCAGGAGTTC TTTCATTTAG CTTGGGGCTT
    GAGGACCAAT GTGAAACTTC CTTCTGTAAA GAATCTAGAG AAGTGATTCC ACAGGACGTT
    TTGCTTCTCA ATAATATACC TACTCAAAGC AAGTGGCTGA AATATCAGAA CATACCCCAG
    TGCAACTTGA CTACTCCAGA CAGAGTTGAT AAGAAAGTAA CAGATGGTTT CTCTGGTAAA
    GTTATTTCTG GGACACATTT AAGTGATATA CGTGAAAGAC AGAGTGGTGC TGTGAATGAA
    AGCTCTTTAG ACTCTATATA TTTGCAAATG ATAAAAGGCA TGCTCTATCA ACAGCAGCAG
    GATTTTAGCT GTCAGGATTT GGTTTTCAGA AAGAAAGCAC TCTCATTGAA TTTAAAGCTG
    ACTTCCCAGA CTGAAAAAAT TCAAAACACG TTAAGAGAGT CTGCTGCCTG CTACATCTAC
    AGTGTAACAG GAGATTTACA GGAGATAAAT GCCTCTGAGC TATGCTTCCC AAGTGGGCAG
    AAAATAAAAT CTGCTTATCT TCCCCAAAGG CAGATTCATA TACCAGCTGT TTTTCAGTCT
    CCTGCTCATT ATAAGCAGAT TTTTACATCT TGTCTCATAG AACACTTAAA TATATTGTTG
    TTTGGGTTGG CACAGAGGTT GCACAAAGCT CTTTCAAAAG TTGACATATC ATTTTACACA
    TCATTAAAGG GAGAGAAACT AAAAAATGTA GAAAGTAATG TACCATCCTG CCATCATCAT
    CAACCTGCAA AACTTGTCAT GGTTAAAAAG GAAGGTCCAA ATAAGGGTCG TCTTTTTTAT
    ACCTGTGATG GACCCAAAGC TGACCGATGT AAATTTTTCA AGTGGCTTGA AGAAGTGACC
    CCAGGATATT TGACACAAGA AGAGTCTCTA CCTAGCATAG TTTTAAGTGA TATAAAGAGT
    ATTGGCTTAT ACTTAAGAAG TCAAAATATA CCCCTCTTTG AGGAATGTCA GCTTTTGGTG
    AGGAAAGGAT TTGATTTTCA AAGAAAACAG TATGGCAAAC TGAAGAAGTT TAATGCTGTA
    AATCCTGAAT TTTATAGTGA ACCTAAAAGC AAACTTTATC TTAAGCTGAG TCGGAGGGAA
    AGTTCTTCAA CTTACAGCAA AAATGATCTT TGGGTGGTTT CAAAAACATT GGACTTTGAA
    TTGGATACTT TTATTGCGTG TAGTGCTTTC TTTGGACCAT CATCTATCAA TGAGGTGGAG
    CTACTACCTT TGAAAGGTTA TTTCCCCTCT AACTGGCCCA CTAACATAGT GGTACATGCA
    TTGTTGGTTT GTAATGCTAG CACTGAGCTG ACAACTTTGA AAAACATTCA GGACTACTTT
    AATCCAGCTA CTCTACCTCT AACACAGTAC TTGTTAACAA TGTCTTCATC AACTATAGTT
    AGCAACAAGA GAGTCAATAA GAGAAAATTT ATCCCACCAG CCTTCTCAAA TATCAATACA
    AAATTTGAAC TTCTCAGCCT AGAAGCCACA TTACAGTTAG CTAGTGAATT AATTCAGGCA
    CACAACTTAA ATAAGGATCA AGCTACAGCT TTAATTCAGA TAGCTCAAAT GATGGCATCA
    CATGAAAATG CTGAAGAAGT GAAGAAAGTA CAAACCCATA CCCTCCCTAT CACAATCATA
    CATGGTGTTT TTGGAGCAGG AAAGAGTTAT TTGTTGGCAG TGGTGATTTT GTTTTTTGTA
    CAGCTATTTG AAAAGAGTGA AGTTCTCACA GTTGGAAATG TAAGACCTTG GAAACTTCTC
    ATTTCTTCTT CCACTAATGT AGCTGTGGAC AGAGTACTTC TTGGGCTTCT CAGTCTTGGT
    TTTGAAAAGT TCGTCAGAGT TGGGAGTGTC AGGAAGATTG CCAAGCCAAT TTTACCTTAT
    AGTTTGCATG CTGGCTCAGA AAATGAAAAT GAACAACTAA AAGAACTACA TGGACTAATG
    AAAGAAGACT TGACTCCTCT GGAAAGGGTC TATGTCAGAA AAAGTATTGA GCAGCATAAA
    CTGGGGACCA ATAAAACCCT GCTGAAGCAG GTACGAGTAG TTGGAGTTAC CTGTGCAGCT
    TGCCCATTCC CATGTATGAA TGATCTTAAA TTTCCTGTAG TTGTGTTGGA TGAGTGTAGT
    CAAATAACTG AACCGGCCTC TCTCCTTCCT ATTGCAAGGG TTCTGATGCA GCCCATGAAA
    ATGGATTGGA ACAAACTCTT TTTGATCGGC TTTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44625
    Clone ID Related Accession (Same CDS sequence) XM_014110062.1
    Accession Version XM_014110062.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5952bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein ZGRF1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726116.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGAAAGCC AGGAATTTAT TGTACTGTAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
    TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAGTTTT ATATGATGAC
    AAAGGAGAAT GTTTGGAGAG TATATTTCTT AAGTGCCTTG AGGTGAAGCC TGGAGATGAC
    TTAGAAGGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATTGCT
    ATTAAACCGG ATGTCATTAA AGAAACATCA GAGTTAAATC CAAGGAAATT TATATCCTCT
    GGGCGGTCCC TTGGATGTCA GCCTGCTGGT TTAAAAAGGA AGTTTACTGG TTTTCAAGGA
    CCACGTCAAG TGCCAAGGAA AATGGTTATT ACAGAAAATG ATGAATCAGC TGCATCACCT
    GAGGCTAAGA AACCTGGCCC TATTTTTCTT TCTCCCTTCT ACAACACACC TCCTTTGTTT
    TCTACTGCTG GCAAGAAAGA TACAAATAAT ATGCCAACAG ACCCTGAGAA CAGTGTCATT
    TACAAGAACA GAGAGAAAAA TGGCTTATAT TATTCTTCTG TTGTCTCTAC TCCATCCTTT
    AAGAATAACC CAGAAATGCT ATGTGAAGAA AATTATTTTT GCTCACCTGT CTATTCTGTA
    AATAAGCATT CAGATTCTTT ACTATCCAAT GAGGCCATAA AAAAAGATGG TTTGACATCT
    CACTGTTTAG GAGTTTCAGA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTAAAG
    TCCAAATCAA CTAGTATGGG TAAAGAACTA GATTCTGGGA TTACAGAACA TTTCCCTCAG
    ATACAACTAC AAGAAAGTGT AAACATTCCT TCTAAACCAA AGTGCATAAT TGAACAAGAC
    AAGTGTGGTG AGGTGAAGAG CACAGACAAT TTATACTACC AGCATCAATC GGAAAATACT
    ATGAGCTATA AAAGCAGATG GGCCATGTAT TTATCCTCAC AGAGTTCACC TGTACGTTCT
    TCTACTGAAG ATGGAAATAA TATAGAAAGG AAACCCAAAA CTCAGGGAGA TAATGTAAAT
    TTAAATTTGA AAGACCTTGT ACAAAAAAGG ATACAGTTTT TTGAAACATG TACTGAAAAG
    GGTAAAAACT ATAATGAAGA CAAGCCAGTA GGTGATAATG ATCAATCTTG CGATCAGGAA
    GTAAAATTGG AAATTCCTTC ATTCTGTAAA AGCAACAGCT TACCATTTAC CTGTAGCAGT
    GTAGAGAATG ATGGCTTATT ATCAGAATCT GACATTCAAA AAAATAATAA AATACCTGTT
    AGTCAGGATG ACCAGATGTG TCTAAAAGGA TCAATTCTCA TTAGAGAAAA TGCTCAGAAG
    ATAAATATAT GTGGAACCCC AGAAAAGTAT GACCAACCCA TGTCGTCCCT GCCAGAATCT
    GAACATCTAC AAATTGAGTC TTCTACAAGT AACAATTCTA GGATCTCTGA TGACTTTACC
    AATATGCTTT CTAAAAGTAA TACTGATAAT GAAAATCTTA ATAGTATTCC TGAACCAATG
    AGTAATGTAA CACAGCCACT TGTGGAGGTG ACTTTTAATC TGAACAATTT TGAAACCAGT
    GACTCTGAGG AGGAATCACA AGAAAATAAC AAAATTTCCC AGGATTCAGA GGGTTGGGTA
    AAGGAAACTC TGGTTAATGA TGGCAGTTCA AGTGTTCAAA AGAGATGTGA AAATATAAGC
    TGTAAGAAAG TTGGCAGTGA ACATTTGTCT CATTTAACAT CTATGGAGGA TAAGCCTACA
    GAGACATTTC CTAAAGAGAC TCTGCTGTCA CAGTTTTGTG ATAAAGCGTG TATGGGTTTT
    GACACAGGAC CTTGGAAAAC TGGAAACAGA AAAGAAATAG AAGAGTATAA TGACACATTA
    AGCAGTTTTG ATTCATCTTT AGAGTGGACT GATGATGTAT ATGTAGATAA TAATGAAGAT
    GCTAATAAAT CTATACAAAA AGTCAGAATT AACTATGATA TTGCTTCACT ACCGACTAGG
    AAACGATCGA CTGCAGAAGT TTTGCAAGCT TTACCAAAGT TGCCTCCTCT GAACCAGGAT
    TCTCAACAGA TTATAAAAGA AAATGAAGTT GAACCAAGTA AACCACTTCA GTCTTTGCAG
    TTCTTTTCCT TTGGAGATAA AGAGACTGCT TTTCTAGATG TTATGCCTAA ACAATCAGAG
    AGAAAAACCT GTGACCCTAA GCCTGTTGAG TTTCAAGGGC ACCAAGTGAA AGGATCTGCT
    ACTAGTGCTG TAATGATGAG AGGACACAGC TCACAGCTAG AATGCGGTCA ATTTCCAGAT
    AGTATTGAGT ATGAAAACTG CACAAGAGAC ACTTGTTTAT TGACACCAAA ATTGACCAGT
    GCTTGTATGC AGATTGATTT CCTGCAGTTC CCAGATGAAA GAAACATTCC AAAAGAAGAT
    ATTAGCTTTA ATATTGAAGA CAATTTTCAA GTGAGAGCTG AGTCAAATAA GGACTCAATT
    GAAAATGACT TGATTTTGGA TTTCTCTCCT AAAAATTCAG AACATTTCTA TCATTATTTG
    GATTCTAGTT CTAAATCAGC AGAGAAGACT TCATGGGAAG CAAATAAGGT AACATCACCA
    GAACAGAAGA TCTCTACATT AAACCCTGAT TCTACTTTTT CTTTGAACTC ACGAGATGAA
    GACTTTGTGC TAGAATTCTC TGAGGAGTCC CTGAAAGAAA GAACTTTACC TGTTATCAAA
    AAATCCAGTT CTTTGGAGAA TAAGAACTTT CAAAGGCTTC CATTAGTAAG TAGAACCCGA
    GTTCCACTAA CTACTTTGCC ACCTGCTGAT TCAGATTCTT GTTCATACGT GATCAACTGT
    GACAGGCAAG AATATTCTGG TTCCTCCCCC ATATTCAACT TATGTGAAGA ATCAGGAGTT
    CTTTCATTTA GCTTGGGGCT TGAGGACCAA TGTGAAACTT CCTTCTGTAA AGAATCTAGA
    GAAGTGATTC CACAGGACGT TTTGCTTCTC AATAATATAC CTACTCAAAG CAAGTGGCTG
    AAATATCAGA ACATACCCCA GTGCAACTTG ACTACTCCAG ACAGAGTTGA TAAGAAAGTA
    ACAGATGGTT TCTCTGGTAA AGTTATTTCT GGGACACATT TAAGTGATAT ACGTGAAAGA
    CAGAGTGGTG CTGTGAATGA AAGCTCTTTA GACTCTATAT ATTTGCAAAT GATAAAAGGC
    ATGCTCTATC AACAGCAGCA GGATTTTAGC TGTCAGGATT TGGTTTTCAG AAAGAAAGCA
    CTCTCATTGA ATTTAAAGCT GACTTCCCAG ACTGAAAAAA TTCAAAACAC GTTAAGAGAG
    TCTGCTGCCT GCTACATCTA CAGTGTAACA GGAGATTTAC AGGAGATAAA TGCCTCTGAG
    CTATGCTTCC CAAGTGGGCA GAAAATAAAA TCTGCTTATC TTCCCCAAAG GCAGATTCAT
    ATACCAGCTG TTTTTCAGTC TCCTGCTCAT TATAAGCAGA TTTTTACATC TTGTCTCATA
    GAACACTTAA ATATATTGTT GTTTGGGTTG GCACAGAGGT TGCACAAAGC TCTTTCAAAA
    GTTGACATAT CATTTTACAC ATCATTAAAG GGAGAGAAAC TAAAAAATGT AGAAAGTAAT
    GTACCATCCT GCCATCATCA TCAACCTGCA AAACTTGTCA TGGTTAAAAA GGAAGGTCCA
    AATAAGGGTC GTCTTTTTTA TACCTGTGAT GGACCCAAAG CTGACCGATG TAAATTTTTC
    AAGTGGCTTG AAGAAGTGAC CCCAGGATAT TTGACACAAG AAGAGTCTCT ACCTAGCATA
    GTTTTAAGTG ATATAAAGAG TATTGGCTTA TACTTAAGAA GTCAAAATAT ACCCCTCTTT
    GAGGAATGTC AGCTTTTGGT GAGGAAAGGA TTTGATTTTC AAAGAAAACA GTATGGCAAA
    CTGAAGAAGT TTAATGCTGT AAATCCTGAA TTTTATAGTG AACCTAAAAG CAAACTTTAT
    CTTAAGCTGA GTCGGAGGGA AAGTTCTTCA ACTTACAGCA AAAATGATCT TTGGGTGGTT
    TCAAAAACAT TGGACTTTGA ATTGGATACT TTTATTGCGT GTAGTGCTTT CTTTGGACCA
    TCATCTATCA ATGAGGTGGA GCTACTACCT TTGAAAGGTT ATTTCCCCTC TAACTGGCCC
    ACTAACATAG TGGTACATGC ATTGTTGGTT TGTAATGCTA GCACTGAGCT GACAACTTTG
    AAAAACATTC AGGACTACTT TAATCCAGCT ACTCTACCTC TAACACAGTA CTTGTTAACA
    ATGTCTTCAT CAACTATAGT TAGCAACAAG AGAGTCAATA AGAGAAAATT TATCCCACCA
    GCCTTCTCAA ATATCAATAC AAAATTTGAA CTTCTCAGCC TAGAAGCCAC ATTACAGTTA
    GCTAGTGAAT TAATTCAGGC ACACAACTTA AATAAGGATC AAGCTACAGC TTTAATTCAG
    ATAGCTCAAA TGATGGCATC ACATGAAAAT GCTGAAGAAG TGAAGAAAGT ACAAACCCAT
    ACCCTCCCTA TCACAATCAT ACATGGTGTT TTTGGAGCAG GAAAGAGTTA TTTGTTGGCA
    GTGGTGATTT TGTTTTTTGT ACAGCTATTT GAAAAGAGTG AAGTTCTCAC AGTTGGAAAT
    GTAAGACCTT GGAAACTTCT CATTTCTTCT TCCACTAATG TAGCTGTGGA CAGAGTACTT
    CTTGGGCTTC TCAGTCTTGG TTTTGAAAAG TTCGTCAGAG TTGGGAGTGT CAGGAAGATT
    GCCAAGCCAA TTTTACCTTA TAGTTTGCAT GCTGGCTCAG AAAATGAAAA TGAACAACTA
    AAAGAACTAC ATGGACTAAT GAAAGAAGAC TTGACTCCTC TGGAAAGGGT CTATGTCAGA
    AAAAGTATTG AGCAGCATAA ACTGGGGACC AATAAAACCC TGCTGAAGCA GGTACGAGTA
    GTTGGAGTTA CCTGTGCAGC TTGCCCATTC CCATGTATGA ATGATCTTAA ATTTCCTGTA
    GTTGTGTTGG ATGAGTGTAG TCAAATAACT GAACCGGCCT CTCTCCTTCC TATTGCAAGG
    TTTGAGTGTG AAAAGCTGAT TCTTGTTGGA GATCCTAAAC AGCTTTCTCC TACTATTCAG
    GGTTCTGATG CAGCCCATGA AAATGGATTG GAACAAACTC TTTTTGATCG GCTTTGCTTA
    ATGGGCCATA AGCCAGTTCT GTTGAGAACC CAGTACCGTT GTCACCCTGC AATCAGTGAT
    ATCTCTAATG ATCTGTTTTA TGAAGGAAAC CTTGTGGATG GCATTTCTGA AACAGAGAGG
    GGCCCTTTAT TGGAATGGCT CCCAACACTA TGTTTCTATA ACATTAAAGG ACCTGAACAG
    ATTGAAAGAG ATAACAGCTT TCATAATGTG GCAGAAGCTG CTTTTACACT CAAGCTGATT
    CAATCACTGA TTGCGAGTGG GATAGCGGGC TCTATGATTG GGGTGATAAC ATTATACAAA
    TCCCAGATGT ACAAGCTTTG TCATTTACTC AGTGCCGTGG ACTTTGACAA TCCTGATATT
    AAAGCTGTGC AGGTATCCAC AGTAGATGCT TTTCAGGGAG CTGAAAAGGA GATCATTATT
    CTGTCCTGTG TAAGGACAAG ACAAGTAGGA TTTATTGATT CAGAAAAAAG AATGAATGTT
    GCACTGACCA GAGGAAGGAG GCATTTATTG ATCGTGGGAA ATTTAGCCTG TTTGCGGAAA
    AATCGACTCT GGGGACGTGT AATCCAACAC TGTGAAGGAA GGAGAGATGG ATTACAACAT
    GCAAGCCAGT GTGAACCACA GCTGAACCAT CTTCTTAAAG AGTATTTTGA AAAACGAGCA
    GAAGAAAAGC AGAAAAAAAG TGAAAAAGAT AAATCTAAAG ATAAATCACA TTCACAAAAA
    GATATGGTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965537.1
    CDS48..5999
    Translation

    Target ORF information:

    RefSeq Version XM_014110062.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110062.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGAAAGCC AGGAATTTAT TGTACTGTAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
    TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAGTTTT ATATGATGAC
    AAAGGAGAAT GTTTGGAGAG TATATTTCTT AAGTGCCTTG AGGTGAAGCC TGGAGATGAC
    TTAGAAGGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATTGCT
    ATTAAACCGG ATGTCATTAA AGAAACATCA GAGTTAAATC CAAGGAAATT TATATCCTCT
    GGGCGGTCCC TTGGATGTCA GCCTGCTGGT TTAAAAAGGA AGTTTACTGG TTTTCAAGGA
    CCACGTCAAG TGCCAAGGAA AATGGTTATT ACAGAAAATG ATGAATCAGC TGCATCACCT
    GAGGCTAAGA AACCTGGCCC TATTTTTCTT TCTCCCTTCT ACAACACACC TCCTTTGTTT
    TCTACTGCTG GCAAGAAAGA TACAAATAAT ATGCCAACAG ACCCTGAGAA CAGTGTCATT
    TACAAGAACA GAGAGAAAAA TGGCTTATAT TATTCTTCTG TTGTCTCTAC TCCATCCTTT
    AAGAATAACC CAGAAATGCT ATGTGAAGAA AATTATTTTT GCTCACCTGT CTATTCTGTA
    AATAAGCATT CAGATTCTTT ACTATCCAAT GAGGCCATAA AAAAAGATGG TTTGACATCT
    CACTGTTTAG GAGTTTCAGA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTAAAG
    TCCAAATCAA CTAGTATGGG TAAAGAACTA GATTCTGGGA TTACAGAACA TTTCCCTCAG
    ATACAACTAC AAGAAAGTGT AAACATTCCT TCTAAACCAA AGTGCATAAT TGAACAAGAC
    AAGTGTGGTG AGGTGAAGAG CACAGACAAT TTATACTACC AGCATCAATC GGAAAATACT
    ATGAGCTATA AAAGCAGATG GGCCATGTAT TTATCCTCAC AGAGTTCACC TGTACGTTCT
    TCTACTGAAG ATGGAAATAA TATAGAAAGG AAACCCAAAA CTCAGGGAGA TAATGTAAAT
    TTAAATTTGA AAGACCTTGT ACAAAAAAGG ATACAGTTTT TTGAAACATG TACTGAAAAG
    GGTAAAAACT ATAATGAAGA CAAGCCAGTA GGTGATAATG ATCAATCTTG CGATCAGGAA
    GTAAAATTGG AAATTCCTTC ATTCTGTAAA AGCAACAGCT TACCATTTAC CTGTAGCAGT
    GTAGAGAATG ATGGCTTATT ATCAGAATCT GACATTCAAA AAAATAATAA AATACCTGTT
    AGTCAGGATG ACCAGATGTG TCTAAAAGGA TCAATTCTCA TTAGAGAAAA TGCTCAGAAG
    ATAAATATAT GTGGAACCCC AGAAAAGTAT GACCAACCCA TGTCGTCCCT GCCAGAATCT
    GAACATCTAC AAATTGAGTC TTCTACAAGT AACAATTCTA GGATCTCTGA TGACTTTACC
    AATATGCTTT CTAAAAGTAA TACTGATAAT GAAAATCTTA ATAGTATTCC TGAACCAATG
    AGTAATGTAA CACAGCCACT TGTGGAGGTG ACTTTTAATC TGAACAATTT TGAAACCAGT
    GACTCTGAGG AGGAATCACA AGAAAATAAC AAAATTTCCC AGGATTCAGA GGGTTGGGTA
    AAGGAAACTC TGGTTAATGA TGGCAGTTCA AGTGTTCAAA AGAGATGTGA AAATATAAGC
    TGTAAGAAAG TTGGCAGTGA ACATTTGTCT CATTTAACAT CTATGGAGGA TAAGCCTACA
    GAGACATTTC CTAAAGAGAC TCTGCTGTCA CAGTTTTGTG ATAAAGCGTG TATGGGTTTT
    GACACAGGAC CTTGGAAAAC TGGAAACAGA AAAGAAATAG AAGAGTATAA TGACACATTA
    AGCAGTTTTG ATTCATCTTT AGAGTGGACT GATGATGTAT ATGTAGATAA TAATGAAGAT
    GCTAATAAAT CTATACAAAA AGTCAGAATT AACTATGATA TTGCTTCACT ACCGACTAGG
    AAACGATCGA CTGCAGAAGT TTTGCAAGCT TTACCAAAGT TGCCTCCTCT GAACCAGGAT
    TCTCAACAGA TTATAAAAGA AAATGAAGTT GAACCAAGTA AACCACTTCA GTCTTTGCAG
    TTCTTTTCCT TTGGAGATAA AGAGACTGCT TTTCTAGATG TTATGCCTAA ACAATCAGAG
    AGAAAAACCT GTGACCCTAA GCCTGTTGAG TTTCAAGGGC ACCAAGTGAA AGGATCTGCT
    ACTAGTGCTG TAATGATGAG AGGACACAGC TCACAGCTAG AATGCGGTCA ATTTCCAGAT
    AGTATTGAGT ATGAAAACTG CACAAGAGAC ACTTGTTTAT TGACACCAAA ATTGACCAGT
    GCTTGTATGC AGATTGATTT CCTGCAGTTC CCAGATGAAA GAAACATTCC AAAAGAAGAT
    ATTAGCTTTA ATATTGAAGA CAATTTTCAA GTGAGAGCTG AGTCAAATAA GGACTCAATT
    GAAAATGACT TGATTTTGGA TTTCTCTCCT AAAAATTCAG AACATTTCTA TCATTATTTG
    GATTCTAGTT CTAAATCAGC AGAGAAGACT TCATGGGAAG CAAATAAGGT AACATCACCA
    GAACAGAAGA TCTCTACATT AAACCCTGAT TCTACTTTTT CTTTGAACTC ACGAGATGAA
    GACTTTGTGC TAGAATTCTC TGAGGAGTCC CTGAAAGAAA GAACTTTACC TGTTATCAAA
    AAATCCAGTT CTTTGGAGAA TAAGAACTTT CAAAGGCTTC CATTAGTAAG TAGAACCCGA
    GTTCCACTAA CTACTTTGCC ACCTGCTGAT TCAGATTCTT GTTCATACGT GATCAACTGT
    GACAGGCAAG AATATTCTGG TTCCTCCCCC ATATTCAACT TATGTGAAGA ATCAGGAGTT
    CTTTCATTTA GCTTGGGGCT TGAGGACCAA TGTGAAACTT CCTTCTGTAA AGAATCTAGA
    GAAGTGATTC CACAGGACGT TTTGCTTCTC AATAATATAC CTACTCAAAG CAAGTGGCTG
    AAATATCAGA ACATACCCCA GTGCAACTTG ACTACTCCAG ACAGAGTTGA TAAGAAAGTA
    ACAGATGGTT TCTCTGGTAA AGTTATTTCT GGGACACATT TAAGTGATAT ACGTGAAAGA
    CAGAGTGGTG CTGTGAATGA AAGCTCTTTA GACTCTATAT ATTTGCAAAT GATAAAAGGC
    ATGCTCTATC AACAGCAGCA GGATTTTAGC TGTCAGGATT TGGTTTTCAG AAAGAAAGCA
    CTCTCATTGA ATTTAAAGCT GACTTCCCAG ACTGAAAAAA TTCAAAACAC GTTAAGAGAG
    TCTGCTGCCT GCTACATCTA CAGTGTAACA GGAGATTTAC AGGAGATAAA TGCCTCTGAG
    CTATGCTTCC CAAGTGGGCA GAAAATAAAA TCTGCTTATC TTCCCCAAAG GCAGATTCAT
    ATACCAGCTG TTTTTCAGTC TCCTGCTCAT TATAAGCAGA TTTTTACATC TTGTCTCATA
    GAACACTTAA ATATATTGTT GTTTGGGTTG GCACAGAGGT TGCACAAAGC TCTTTCAAAA
    GTTGACATAT CATTTTACAC ATCATTAAAG GGAGAGAAAC TAAAAAATGT AGAAAGTAAT
    GTACCATCCT GCCATCATCA TCAACCTGCA AAACTTGTCA TGGTTAAAAA GGAAGGTCCA
    AATAAGGGTC GTCTTTTTTA TACCTGTGAT GGACCCAAAG CTGACCGATG TAAATTTTTC
    AAGTGGCTTG AAGAAGTGAC CCCAGGATAT TTGACACAAG AAGAGTCTCT ACCTAGCATA
    GTTTTAAGTG ATATAAAGAG TATTGGCTTA TACTTAAGAA GTCAAAATAT ACCCCTCTTT
    GAGGAATGTC AGCTTTTGGT GAGGAAAGGA TTTGATTTTC AAAGAAAACA GTATGGCAAA
    CTGAAGAAGT TTAATGCTGT AAATCCTGAA TTTTATAGTG AACCTAAAAG CAAACTTTAT
    CTTAAGCTGA GTCGGAGGGA AAGTTCTTCA ACTTACAGCA AAAATGATCT TTGGGTGGTT
    TCAAAAACAT TGGACTTTGA ATTGGATACT TTTATTGCGT GTAGTGCTTT CTTTGGACCA
    TCATCTATCA ATGAGGTGGA GCTACTACCT TTGAAAGGTT ATTTCCCCTC TAACTGGCCC
    ACTAACATAG TGGTACATGC ATTGTTGGTT TGTAATGCTA GCACTGAGCT GACAACTTTG
    AAAAACATTC AGGACTACTT TAATCCAGCT ACTCTACCTC TAACACAGTA CTTGTTAACA
    ATGTCTTCAT CAACTATAGT TAGCAACAAG AGAGTCAATA AGAGAAAATT TATCCCACCA
    GCCTTCTCAA ATATCAATAC AAAATTTGAA CTTCTCAGCC TAGAAGCCAC ATTACAGTTA
    GCTAGTGAAT TAATTCAGGC ACACAACTTA AATAAGGATC AAGCTACAGC TTTAATTCAG
    ATAGCTCAAA TGATGGCATC ACATGAAAAT GCTGAAGAAG TGAAGAAAGT ACAAACCCAT
    ACCCTCCCTA TCACAATCAT ACATGGTGTT TTTGGAGCAG GAAAGAGTTA TTTGTTGGCA
    GTGGTGATTT TGTTTTTTGT ACAGCTATTT GAAAAGAGTG AAGTTCTCAC AGTTGGAAAT
    GTAAGACCTT GGAAACTTCT CATTTCTTCT TCCACTAATG TAGCTGTGGA CAGAGTACTT
    CTTGGGCTTC TCAGTCTTGG TTTTGAAAAG TTCGTCAGAG TTGGGAGTGT CAGGAAGATT
    GCCAAGCCAA TTTTACCTTA TAGTTTGCAT GCTGGCTCAG AAAATGAAAA TGAACAACTA
    AAAGAACTAC ATGGACTAAT GAAAGAAGAC TTGACTCCTC TGGAAAGGGT CTATGTCAGA
    AAAAGTATTG AGCAGCATAA ACTGGGGACC AATAAAACCC TGCTGAAGCA GGTACGAGTA
    GTTGGAGTTA CCTGTGCAGC TTGCCCATTC CCATGTATGA ATGATCTTAA ATTTCCTGTA
    GTTGTGTTGG ATGAGTGTAG TCAAATAACT GAACCGGCCT CTCTCCTTCC TATTGCAAGG
    TTTGAGTGTG AAAAGCTGAT TCTTGTTGGA GATCCTAAAC AGCTTTCTCC TACTATTCAG
    GGTTCTGATG CAGCCCATGA AAATGGATTG GAACAAACTC TTTTTGATCG GCTTTGCTTA
    ATGGGCCATA AGCCAGTTCT GTTGAGAACC CAGTACCGTT GTCACCCTGC AATCAGTGAT
    ATCTCTAATG ATCTGTTTTA TGAAGGAAAC CTTGTGGATG GCATTTCTGA AACAGAGAGG
    GGCCCTTTAT TGGAATGGCT CCCAACACTA TGTTTCTATA ACATTAAAGG ACCTGAACAG
    ATTGAAAGAG ATAACAGCTT TCATAATGTG GCAGAAGCTG CTTTTACACT CAAGCTGATT
    CAATCACTGA TTGCGAGTGG GATAGCGGGC TCTATGATTG GGGTGATAAC ATTATACAAA
    TCCCAGATGT ACAAGCTTTG TCATTTACTC AGTGCCGTGG ACTTTGACAA TCCTGATATT
    AAAGCTGTGC AGGTATCCAC AGTAGATGCT TTTCAGGGAG CTGAAAAGGA GATCATTATT
    CTGTCCTGTG TAAGGACAAG ACAAGTAGGA TTTATTGATT CAGAAAAAAG AATGAATGTT
    GCACTGACCA GAGGAAGGAG GCATTTATTG ATCGTGGGAA ATTTAGCCTG TTTGCGGAAA
    AATCGACTCT GGGGACGTGT AATCCAACAC TGTGAAGGAA GGAGAGATGG ATTACAACAT
    GCAAGCCAGT GTGAACCACA GCTGAACCAT CTTCTTAAAG AGTATTTTGA AAAACGAGCA
    GAAGAAAAGC AGAAAAAAAG TGAAAAAGAT AAATCTAAAG ATAAATCACA TTCACAAAAA
    GATATGGTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284011
    Clone ID Related Accession (Same CDS sequence) XM_022413412.1
    Accession Version XM_022413412.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein ZGRF1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGAAAGCC AGGAATTTAT TGTACTGTAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
    TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGTGAAGCC TGGAGATGAC
    TTAGAAGGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATTGCT
    ATTAAACCGG ATGTCATTAA AGAAACATCA GAGTTAAATC CAAGGAAATT TATATCCTCT
    GGGCGGTCCC TTGGATGTCA GCCTGCTGGT TTAAAAAGGA AGTTTACTGG TTTTCAAGGA
    CCACGTCAAG TGCCAAGGAA AATGGTTATT ACAGAAAATG ATGAATCAGC TGCATCACCT
    GAGGCTAAGA AACCTGGCCC TATTTTTCTT TCTCCCTTCT ACAACACACC TCCTTTGTTT
    TCTACTGCTG GCAAGAAAGA TACAAATAAT ATGCCAACAG ACCCTGAGAA CAGTGTCATT
    TACAAGAACA GAGAGAAAAA TGGCTTATAT TATTCTTCTG TTGTCTCTAC TCCATCCTTT
    AAGAATAACC CAGAAATGCT ATGTGAAGAA AATTATTTTT GCTCACCTGT CTATTCTGTA
    AATAAGCATT CAGATTCTTT ACTATCCAAT GAGGCCATAA AAAAAGATGG TTTGACATCT
    CACTGTTTAG GAGTTTCAGA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTAAAG
    TCCAAATCAA CTAGTATGGG TAAAGAACTA GATTCTGGGA TTACAGAACA TTTCCCTCAG
    ATACAACTAC AAGAAAGTGT AAACATTCCT TCTAAACCAA AGTGCATAAT TGAACAAGAC
    AAGTGTGGTG AGGTGAAGAG CACAGACAAT TTATACTACC AGCATCAATC GGAAAATACT
    ATGAGCTATA AAAGCAGATG GGCCATGTAT TTATCCTCAC AGAGTTCACC TGTACGTTCT
    TCTACTGAAG ATGGAAATAA TATAGAAAGG AAACCCAAAA CTCAGGGAGA TAATGTAAAT
    TTAAATTTGA AAGACCTTGT ACAAAAAAGG ATACAGTTTT TTGAAACATG TACTGAAAAG
    GGTAAAAACT ATAATGAAGA CAAGCCAGTA GGTGATAATG ATCAATCTTG CGATCAGGAA
    GTAAAATTGG AAATTCCTTC ATTCTGTAAA AGCAACAGCT TACCATTTAC CTGTAGCAGT
    GTAGAGAATG ATGGCTTATT ATCAGAATCT GACATTCAAA AAAATAATAA AATACCTGTT
    AGTCAGGATG ACCAGATGTG TCTAAAAGGA TCAATTCTCA TTAGAGAAAA TGCTCAGAAG
    ATAAATATAT GTGGAACCCC AGAAAAGTAT GACCAACCCA TGTCGTCCCT GCCAGAATCT
    GAACATCTAC AAATTGAGTC TTCTACAAGT AACAATTCTA GGATCTCTGA TGACTTTACC
    AATATGCTTT CTAAAAGTAA TACTGATAAT GAAAATCTTA ATAGTATTCC TGAACCAATG
    AGTAATGTAA CACAGCCACT TGTGGAGGTG ACTTTTAATC TGAACAATTT TGAAACCAGT
    GACTCTGAGG AGGAATCACA AGAAAATAAC AAAATTTCCC AGGATTCAGA GGGTTGGGTA
    AAGGAAACTC TGGTTAATGA TGGCAGTTCA AGTGTTCAAA AGAGATGTGA AAATATAAGC
    TGTAAGAAAG TTGGCAGTGA ACATTTGTCT CATTTAACAT CTATGGAGGA TAAGCCTACA
    GAGACATTTC CTAAAGAGAC TCTGCTGTCA CAGTTTTGTG ATAAAGCGTG TATGGGTTTT
    GACACAGGAC CTTGGAAAAC TGGAAACAGA AAAGAAATAG AAGAGTATAA TGACACATTA
    AGCAGTTTTG ATTCATCTTT AGAGTGGACT GATGATGTAT ATGTAGATAA TAATGAAGAT
    GCTAATAAAT CTATACAAAA AGTCAGAATT AACTATGATA TTGCTTCACT ACCGAGTAAG
    TCTAAAGGTA TAAGCTCAAA TTTACATATT CCTCAGTTAA ACATAGCCAC TAATCAGATT
    CCTAAAAACA GCCTATTCTC GGAACAGTCT GGGCTTAAGC CTTTTATAAT GGGAAATAAA
    TTAGACAAAA ATGATGAACA GGTTTTACTG TCAACTTCTA GCAGTGACAT CAGTATCCAA
    CAATTAAATG TTAATCAGAA TCACTCTGAA GAACCTGTTG CTCTTGGTAA ATCAAATACC
    CAAGTTTCCA ATTCTTTGTT GTACCTTCTC GGAGAAAAGC ATCCTATTTC CAAAGAAACA
    GAAGCGTGTA TTCCTGAATC CGAAGATTTG GGAAGGATTA GAAGTTTAGA CCATGACCAT
    ATTGAAGTAG AACCTGCTGG AGAAAGCAAA CAACATTGGA ATAGTCCTAG GAATCCTTCA
    GAACTCTCTG GATTAATAAA TAACATTTCC CTTTTAAAGT CATTGTCTGA ACATAGTACA
    GCTTTAGAAG GCTTGGAAAT ATTGAAAAAG AAAAATACTA CCTTTAAACA ACAAGGAACT
    CTACAGACAT ATAATCCAGA TAGTAATTCT GAAGCTAGGA AACGATCGAC TGCAGAAGTT
    TTGCAAGCTT TACCAAAGTT GCCTCCTCTG AACCAGGATT CTCAACAGAT TATAAAAGAA
    AATGAAGTTG AACCAAGTAA ACCACTTCAG TCTTTGCAGT TCTTTTCCTT TGGAGATAAA
    GAGACTGCTT TTCTAGATGT TATGCCTAAA CAATCAGAGA GAAAAACCTG TGACCCTAAG
    CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA GGATCTGCTA CTAGTGCTGT AATGATGAGA
    GGACACAGCT CACAGCTAGA ATGCGGTCAA TTTCCAGATA GTATTGAGTA TGAAAACTGC
    ACAAGAGACA CTTGTTTATT GACACCAAAA TTGACCAGTG CTTGTATGCA GATTGATTTC
    CTGCAGTTCC CAGATGAAAG AAACATTCCA AAAGAAGATA TTAGCTTTAA TATTGAAGAC
    AATTTTCAAG TGAGAGCTGA GTCAAATAAG GACTCAATTG AAAATGACTT GATTTTGGAT
    TTCTCTCCTA AAAATTCAGA ACATTTCTAT CATTATTTGG ATTCTAGTTC TAAATCAGCA
    GAGAAGACTT CATGGGAAGC AAATAAGGTA ACATCACCAG AACAGAAGAT CTCTACATTA
    AACCCTGATT CTACTTTTTC TTTGAACTCA CGAGATGAAG ACTTTGTGCT AGAATTCTCT
    GAGGAGTCCC TGAAAGAAAG AACTTTACCT GTTATCAAAA AATCCAGTTC TTTGGAGAAT
    AAGAACTTTC AAAGGCTTCC ATTAGTAAGT AGAACCCGAG TTCCACTAAC TACTTTGCCA
    CCTGCTGATT CAGATTCTTG TTCATACGTG ATCAACTGTG ACAGGCAAGA ATATTCTGGT
    TCCTCCCCCA TATTCAACTT ATGTGAAGAA TCAGGAGTTC TTTCATTTAG CTTGGGGCTT
    GAGGACCAAT GTGAAACTTC CTTCTGTAAA GAATCTAGAG AAGTGATTCC ACAGGACGTT
    TTGCTTCTCA ATAATATACC TACTCAAAGC AAGTGGCTGA AATATCAGAA CATACCCCAG
    TGCAACTTGA CTACTCCAGA CAGAGTTGAT AAGAAAGTAA CAGATGGTTT CTCTGGTAAA
    GTTATTTCTG GGACACATTT AAGTGATATA CGTGAAAGAC AGAGTGGTGC TGTGAATGAA
    AGCTCTTTAG ACTCTATATA TTTGCAAATG ATAAAAGGCA TGCTCTATCA ACAGCAGCAG
    GATTTTAGCT GTCAGGATTT GGTTTTCAGA AAGAAAGCAC TCTCATTGAA TTTAAAGCTG
    ACTTCCCAGA CTGAAAAAAT TCAAAACACG TTAAGAGAGT CTGCTGCCTG CTACATCTAC
    AGTGTAACAG GAGATTTACA GGAGATAAAT GCCTCTGAGC TATGCTTCCC AAGTGGGCAG
    AAAATAAAAT CTGCTTATCT TCCCCAAAGG CAGATTCATA TACCAGCTGT TTTTCAGTCT
    CCTGCTCATT ATAAGCAGAT TTTTACATCT TGTCTCATAG AACACTTAAA TATATTGTTG
    TTTGGGTTGG CACAGAGGTT GCACAAAGCT CTTTCAAAAG TTGACATATC ATTTTACACA
    TCATTAAAGG GAGAGAAACT AAAAAATGTA GAAAGTAATG TACCATCCTG CCATCATCAT
    CAACCTGCAA AACTTGTCAT GGTTAAAAAG GAAGGTCCAA ATAAGGGTCG TCTTTTTTAT
    ACCTGTGATG GACCCAAAGC TGACCGATGT AAATTTTTCA AGTGGCTTGA AGAAGTGACC
    CCAGGATATT TGACACAAGA AGAGTCTCTA CCTAGCATAG TTTTAAGTGA TATAAAGAGT
    ATTGGCTTAT ACTTAAGAAG TCAAAATATA CCCCTCTTTG AGGAATGTCA GCTTTTGGTG
    AGGAAAGGAT TTGATTTTCA AAGAAAACAG TATGGCAAAC TGAAGAAGTT TAATGCTGTA
    AATCCTGAAT TTTATAGTGA ACCTAAAAGC AAACTTTATC TTAAGCTGAG TCGGAGGGAA
    AGTTCTTCAA CTTACAGCAA AAATGATCTT TGGGTGGTTT CAAAAACATT GGACTTTGAA
    TTGGATACTT TTATTGCGTG TAGTGCTTTC TTTGGACCAT CATCTATCAA TGAGGTGGAG
    CTACTACCTT TGAAAGGTTA TTTCCCCTCT AACTGGCCCA CTAACATAGT GGTACATGCA
    TTGTTGGTTT GTAATGCTAG CACTGAGCTG ACAACTTTGA AAAACATTCA GGACTACTTT
    AATCCAGCTA CTCTACCTCT AACACAGTAC TTGTTAACAA TGTCTTCATC AACTATAGTT
    AGCAACAAGA GAGTCAATAA GAGAAAATTT ATCCCACCAG CCTTCTCAAA TATCAATACA
    AAATTTGAAC TTCTCAGCCT AGAAGCCACA TTACAGTTAG CTAGTGAATT AATTCAGGCA
    CACAACTTAA ATAAGGATCA AGCTACAGCT TTAATTCAGA TAGCTCAAAT GATGGCATCA
    CATGAAAATG CTGAAGAAGT GAAGAAAGTA CAAACCCATA CCCTCCCTAT CACAATCATA
    CATGGTGTTT TTGGAGCAGG AAAGAGTTAT TTGTTGGCAG TGGTGATTTT GTTTTTTGTA
    CAGCTATTTG AAAAGAGTGA AGTTCTCACA GTTGGAAATG TAAGACCTTG GAAACTTCTC
    ATTTCTTCTT CCACTAATGT AGCTGTGGAC AGAGTACTTC TTGGGCTTCT CAGTCTTGGT
    TTTGAAAAGT TCGTCAGAGT TGGGAGTGTC AGGAAGATTG CCAAGCCAAT TTTACCTTAT
    AGTTTGCATG CTGGCTCAGA AAATGAAAAT GAACAACTAA AAGAACTACA TGGACTAATG
    AAAGAAGACT TGACTCCTCT GGAAAGGGTC TATGTCAGAA AAAGTATTGA GCAGCATAAA
    CTGGGGACCA ATAAAACCCT GCTGAAGCAG GTACGAGTAG TTGGAGTTAC CTGTGCAGCT
    TGCCCATTCC CATGTATGAA TGATCTTAAA TTTCCTGTAG TTGTGTTGGA TGAGTGTAGT
    CAAATAACTG AACCGGCCTC TCTCCTTCCT ATTGCAAGGT TTGAGTGTGA AAAGCTGATT
    CTTGTTGGAG ATCCTAAACA GCTTTCTCCT ACTATTCAGG GTTCTGATGC AGCCCATGAA
    AATGGATTGG AACAAACTCT TTTTGATCGG CTTTGCTTAA TGGGCCATAA GCCAGTTCTG
    TTGAGAACCC AGTACCGTTG TCACCCTGCA ATCAGTGATA TCTCTAATGA TCTGTTTTAT
    GAAGGAAACC TTGTGGATGG CATTTCTGAA ACAGAGAGGG GCCCTTTATT GGAATGGCTC
    CCAACACTAT GTTTCTATAA CATTAAAGGA CCTGAACAGA TTGAAAGAGA TAACAGCTTT
    CATAATGTGG CAGAAGCTGC TTTTACACTC AAGCTGATTC AATCACTGAT TGCGAGTGGG
    ATAGCGGGCT CTATGATTGG GGTGATAACA TTATACAAAT CCCAGATGTA CAAGCTTTGT
    CATTTACTCA GTGCCGTGGA CTTTGACAAT CCTGATATTA AAGCTGTGCA GGTATCCACA
    GTAGATGCTT TTCAGGGAGC TGAAAAGGAG ATCATTATTC TGTCCTGTGT AAGGACAAGA
    CAAGTAGGAT TTATTGATTC AGAAAAAAGA ATGAATGTTG CACTGACCAG AGGAAGGAGG
    CATTTATTGA TCGTGGGAAA TTTAGCCTGT TTGCGGAAAA ATCGACTCTG GGGACGTGTA
    ATCCAACACT GTGAAGGAAG GAGAGATGGA TTACAACATG CAAGCCAGTG TGAACCACAG
    CTGAACCATC TTCTTAAAGA GTATTTTGAA AAACGAGCAG AAGAAAAGCA GAAAAAAAGT
    GAAAAAGATA AATCTAAAGA TAAATCACAT TCACAAAAAG ATATGGTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269120.1
    CDS316..6786
    Translation

    Target ORF information:

    RefSeq Version XM_022413412.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris zinc finger GRF-type containing 1 (ZGRF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413412.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGAAAGCC AGGAATTTAT TGTACTGTAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
    TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGTGAAGCC TGGAGATGAC
    TTAGAAGGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATTGCT
    ATTAAACCGG ATGTCATTAA AGAAACATCA GAGTTAAATC CAAGGAAATT TATATCCTCT
    GGGCGGTCCC TTGGATGTCA GCCTGCTGGT TTAAAAAGGA AGTTTACTGG TTTTCAAGGA
    CCACGTCAAG TGCCAAGGAA AATGGTTATT ACAGAAAATG ATGAATCAGC TGCATCACCT
    GAGGCTAAGA AACCTGGCCC TATTTTTCTT TCTCCCTTCT ACAACACACC TCCTTTGTTT
    TCTACTGCTG GCAAGAAAGA TACAAATAAT ATGCCAACAG ACCCTGAGAA CAGTGTCATT
    TACAAGAACA GAGAGAAAAA TGGCTTATAT TATTCTTCTG TTGTCTCTAC TCCATCCTTT
    AAGAATAACC CAGAAATGCT ATGTGAAGAA AATTATTTTT GCTCACCTGT CTATTCTGTA
    AATAAGCATT CAGATTCTTT ACTATCCAAT GAGGCCATAA AAAAAGATGG TTTGACATCT
    CACTGTTTAG GAGTTTCAGA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTAAAG
    TCCAAATCAA CTAGTATGGG TAAAGAACTA GATTCTGGGA TTACAGAACA TTTCCCTCAG
    ATACAACTAC AAGAAAGTGT AAACATTCCT TCTAAACCAA AGTGCATAAT TGAACAAGAC
    AAGTGTGGTG AGGTGAAGAG CACAGACAAT TTATACTACC AGCATCAATC GGAAAATACT
    ATGAGCTATA AAAGCAGATG GGCCATGTAT TTATCCTCAC AGAGTTCACC TGTACGTTCT
    TCTACTGAAG ATGGAAATAA TATAGAAAGG AAACCCAAAA CTCAGGGAGA TAATGTAAAT
    TTAAATTTGA AAGACCTTGT ACAAAAAAGG ATACAGTTTT TTGAAACATG TACTGAAAAG
    GGTAAAAACT ATAATGAAGA CAAGCCAGTA GGTGATAATG ATCAATCTTG CGATCAGGAA
    GTAAAATTGG AAATTCCTTC ATTCTGTAAA AGCAACAGCT TACCATTTAC CTGTAGCAGT
    GTAGAGAATG ATGGCTTATT ATCAGAATCT GACATTCAAA AAAATAATAA AATACCTGTT
    AGTCAGGATG ACCAGATGTG TCTAAAAGGA TCAATTCTCA TTAGAGAAAA TGCTCAGAAG
    ATAAATATAT GTGGAACCCC AGAAAAGTAT GACCAACCCA TGTCGTCCCT GCCAGAATCT
    GAACATCTAC AAATTGAGTC TTCTACAAGT AACAATTCTA GGATCTCTGA TGACTTTACC
    AATATGCTTT CTAAAAGTAA TACTGATAAT GAAAATCTTA ATAGTATTCC TGAACCAATG
    AGTAATGTAA CACAGCCACT TGTGGAGGTG ACTTTTAATC TGAACAATTT TGAAACCAGT
    GACTCTGAGG AGGAATCACA AGAAAATAAC AAAATTTCCC AGGATTCAGA GGGTTGGGTA
    AAGGAAACTC TGGTTAATGA TGGCAGTTCA AGTGTTCAAA AGAGATGTGA AAATATAAGC
    TGTAAGAAAG TTGGCAGTGA ACATTTGTCT CATTTAACAT CTATGGAGGA TAAGCCTACA
    GAGACATTTC CTAAAGAGAC TCTGCTGTCA CAGTTTTGTG ATAAAGCGTG TATGGGTTTT
    GACACAGGAC CTTGGAAAAC TGGAAACAGA AAAGAAATAG AAGAGTATAA TGACACATTA
    AGCAGTTTTG ATTCATCTTT AGAGTGGACT GATGATGTAT ATGTAGATAA TAATGAAGAT
    GCTAATAAAT CTATACAAAA AGTCAGAATT AACTATGATA TTGCTTCACT ACCGAGTAAG
    TCTAAAGGTA TAAGCTCAAA TTTACATATT CCTCAGTTAA ACATAGCCAC TAATCAGATT
    CCTAAAAACA GCCTATTCTC GGAACAGTCT GGGCTTAAGC CTTTTATAAT GGGAAATAAA
    TTAGACAAAA ATGATGAACA GGTTTTACTG TCAACTTCTA GCAGTGACAT CAGTATCCAA
    CAATTAAATG TTAATCAGAA TCACTCTGAA GAACCTGTTG CTCTTGGTAA ATCAAATACC
    CAAGTTTCCA ATTCTTTGTT GTACCTTCTC GGAGAAAAGC ATCCTATTTC CAAAGAAACA
    GAAGCGTGTA TTCCTGAATC CGAAGATTTG GGAAGGATTA GAAGTTTAGA CCATGACCAT
    ATTGAAGTAG AACCTGCTGG AGAAAGCAAA CAACATTGGA ATAGTCCTAG GAATCCTTCA
    GAACTCTCTG GATTAATAAA TAACATTTCC CTTTTAAAGT CATTGTCTGA ACATAGTACA
    GCTTTAGAAG GCTTGGAAAT ATTGAAAAAG AAAAATACTA CCTTTAAACA ACAAGGAACT
    CTACAGACAT ATAATCCAGA TAGTAATTCT GAAGCTAGGA AACGATCGAC TGCAGAAGTT
    TTGCAAGCTT TACCAAAGTT GCCTCCTCTG AACCAGGATT CTCAACAGAT TATAAAAGAA
    AATGAAGTTG AACCAAGTAA ACCACTTCAG TCTTTGCAGT TCTTTTCCTT TGGAGATAAA
    GAGACTGCTT TTCTAGATGT TATGCCTAAA CAATCAGAGA GAAAAACCTG TGACCCTAAG
    CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA GGATCTGCTA CTAGTGCTGT AATGATGAGA
    GGACACAGCT CACAGCTAGA ATGCGGTCAA TTTCCAGATA GTATTGAGTA TGAAAACTGC
    ACAAGAGACA CTTGTTTATT GACACCAAAA TTGACCAGTG CTTGTATGCA GATTGATTTC
    CTGCAGTTCC CAGATGAAAG AAACATTCCA AAAGAAGATA TTAGCTTTAA TATTGAAGAC
    AATTTTCAAG TGAGAGCTGA GTCAAATAAG GACTCAATTG AAAATGACTT GATTTTGGAT
    TTCTCTCCTA AAAATTCAGA ACATTTCTAT CATTATTTGG ATTCTAGTTC TAAATCAGCA
    GAGAAGACTT CATGGGAAGC AAATAAGGTA ACATCACCAG AACAGAAGAT CTCTACATTA
    AACCCTGATT CTACTTTTTC TTTGAACTCA CGAGATGAAG ACTTTGTGCT AGAATTCTCT
    GAGGAGTCCC TGAAAGAAAG AACTTTACCT GTTATCAAAA AATCCAGTTC TTTGGAGAAT
    AAGAACTTTC AAAGGCTTCC ATTAGTAAGT AGAACCCGAG TTCCACTAAC TACTTTGCCA
    CCTGCTGATT CAGATTCTTG TTCATACGTG ATCAACTGTG ACAGGCAAGA ATATTCTGGT
    TCCTCCCCCA TATTCAACTT ATGTGAAGAA TCAGGAGTTC TTTCATTTAG CTTGGGGCTT
    GAGGACCAAT GTGAAACTTC CTTCTGTAAA GAATCTAGAG AAGTGATTCC ACAGGACGTT
    TTGCTTCTCA ATAATATACC TACTCAAAGC AAGTGGCTGA AATATCAGAA CATACCCCAG
    TGCAACTTGA CTACTCCAGA CAGAGTTGAT AAGAAAGTAA CAGATGGTTT CTCTGGTAAA
    GTTATTTCTG GGACACATTT AAGTGATATA CGTGAAAGAC AGAGTGGTGC TGTGAATGAA
    AGCTCTTTAG ACTCTATATA TTTGCAAATG ATAAAAGGCA TGCTCTATCA ACAGCAGCAG
    GATTTTAGCT GTCAGGATTT GGTTTTCAGA AAGAAAGCAC TCTCATTGAA TTTAAAGCTG
    ACTTCCCAGA CTGAAAAAAT TCAAAACACG TTAAGAGAGT CTGCTGCCTG CTACATCTAC
    AGTGTAACAG GAGATTTACA GGAGATAAAT GCCTCTGAGC TATGCTTCCC AAGTGGGCAG
    AAAATAAAAT CTGCTTATCT TCCCCAAAGG CAGATTCATA TACCAGCTGT TTTTCAGTCT
    CCTGCTCATT ATAAGCAGAT TTTTACATCT TGTCTCATAG AACACTTAAA TATATTGTTG
    TTTGGGTTGG CACAGAGGTT GCACAAAGCT CTTTCAAAAG TTGACATATC ATTTTACACA
    TCATTAAAGG GAGAGAAACT AAAAAATGTA GAAAGTAATG TACCATCCTG CCATCATCAT
    CAACCTGCAA AACTTGTCAT GGTTAAAAAG GAAGGTCCAA ATAAGGGTCG TCTTTTTTAT
    ACCTGTGATG GACCCAAAGC TGACCGATGT AAATTTTTCA AGTGGCTTGA AGAAGTGACC
    CCAGGATATT TGACACAAGA AGAGTCTCTA CCTAGCATAG TTTTAAGTGA TATAAAGAGT
    ATTGGCTTAT ACTTAAGAAG TCAAAATATA CCCCTCTTTG AGGAATGTCA GCTTTTGGTG
    AGGAAAGGAT TTGATTTTCA AAGAAAACAG TATGGCAAAC TGAAGAAGTT TAATGCTGTA
    AATCCTGAAT TTTATAGTGA ACCTAAAAGC AAACTTTATC TTAAGCTGAG TCGGAGGGAA
    AGTTCTTCAA CTTACAGCAA AAATGATCTT TGGGTGGTTT CAAAAACATT GGACTTTGAA
    TTGGATACTT TTATTGCGTG TAGTGCTTTC TTTGGACCAT CATCTATCAA TGAGGTGGAG
    CTACTACCTT TGAAAGGTTA TTTCCCCTCT AACTGGCCCA CTAACATAGT GGTACATGCA
    TTGTTGGTTT GTAATGCTAG CACTGAGCTG ACAACTTTGA AAAACATTCA GGACTACTTT
    AATCCAGCTA CTCTACCTCT AACACAGTAC TTGTTAACAA TGTCTTCATC AACTATAGTT
    AGCAACAAGA GAGTCAATAA GAGAAAATTT ATCCCACCAG CCTTCTCAAA TATCAATACA
    AAATTTGAAC TTCTCAGCCT AGAAGCCACA TTACAGTTAG CTAGTGAATT AATTCAGGCA
    CACAACTTAA ATAAGGATCA AGCTACAGCT TTAATTCAGA TAGCTCAAAT GATGGCATCA
    CATGAAAATG CTGAAGAAGT GAAGAAAGTA CAAACCCATA CCCTCCCTAT CACAATCATA
    CATGGTGTTT TTGGAGCAGG AAAGAGTTAT TTGTTGGCAG TGGTGATTTT GTTTTTTGTA
    CAGCTATTTG AAAAGAGTGA AGTTCTCACA GTTGGAAATG TAAGACCTTG GAAACTTCTC
    ATTTCTTCTT CCACTAATGT AGCTGTGGAC AGAGTACTTC TTGGGCTTCT CAGTCTTGGT
    TTTGAAAAGT TCGTCAGAGT TGGGAGTGTC AGGAAGATTG CCAAGCCAAT TTTACCTTAT
    AGTTTGCATG CTGGCTCAGA AAATGAAAAT GAACAACTAA AAGAACTACA TGGACTAATG
    AAAGAAGACT TGACTCCTCT GGAAAGGGTC TATGTCAGAA AAAGTATTGA GCAGCATAAA
    CTGGGGACCA ATAAAACCCT GCTGAAGCAG GTACGAGTAG TTGGAGTTAC CTGTGCAGCT
    TGCCCATTCC CATGTATGAA TGATCTTAAA TTTCCTGTAG TTGTGTTGGA TGAGTGTAGT
    CAAATAACTG AACCGGCCTC TCTCCTTCCT ATTGCAAGGT TTGAGTGTGA AAAGCTGATT
    CTTGTTGGAG ATCCTAAACA GCTTTCTCCT ACTATTCAGG GTTCTGATGC AGCCCATGAA
    AATGGATTGG AACAAACTCT TTTTGATCGG CTTTGCTTAA TGGGCCATAA GCCAGTTCTG
    TTGAGAACCC AGTACCGTTG TCACCCTGCA ATCAGTGATA TCTCTAATGA TCTGTTTTAT
    GAAGGAAACC TTGTGGATGG CATTTCTGAA ACAGAGAGGG GCCCTTTATT GGAATGGCTC
    CCAACACTAT GTTTCTATAA CATTAAAGGA CCTGAACAGA TTGAAAGAGA TAACAGCTTT
    CATAATGTGG CAGAAGCTGC TTTTACACTC AAGCTGATTC AATCACTGAT TGCGAGTGGG
    ATAGCGGGCT CTATGATTGG GGTGATAACA TTATACAAAT CCCAGATGTA CAAGCTTTGT
    CATTTACTCA GTGCCGTGGA CTTTGACAAT CCTGATATTA AAGCTGTGCA GGTATCCACA
    GTAGATGCTT TTCAGGGAGC TGAAAAGGAG ATCATTATTC TGTCCTGTGT AAGGACAAGA
    CAAGTAGGAT TTATTGATTC AGAAAAAAGA ATGAATGTTG CACTGACCAG AGGAAGGAGG
    CATTTATTGA TCGTGGGAAA TTTAGCCTGT TTGCGGAAAA ATCGACTCTG GGGACGTGTA
    ATCCAACACT GTGAAGGAAG GAGAGATGGA TTACAACATG CAAGCCAGTG TGAACCACAG
    CTGAACCATC TTCTTAAAGA GTATTTTGAA AAACGAGCAG AAGAAAAGCA GAAAAAAAGT
    GAAAAAGATA AATCTAAAGA TAAATCACAT TCACAAAAAG ATATGGTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284012
    Clone ID Related Accession (Same CDS sequence) XM_022413413.1
    Accession Version XM_022413413.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein ZGRF1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGAAAGCC AGGAATTTAT TGTACTGTAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
    TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAGTTTT ATATGATGAC
    AAAGGAGAAT GTTTGGAGAG TATATTTCTT AAGTGCCTTG AGGTGAAGCC TGGAGATGAC
    TTAGAAGGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATTGCT
    ATTAAACCGG ATGTCATTAA AGAAACATCA GAGTTAAATC CAAGGAAATT TATATCCTCT
    GGGCGGTCCC TTGGATGTCA GCCTGCTGGT TTAAAAAGGA AGTTTACTGG TTTTCAAGGA
    CCACGTCAAG TGCCAAGGAA AATGGTTATT ACAGAAAATG ATGAATCAGC TGCATCACCT
    GAGGCTAAGA AACCTGGCCC TATTTTTCTT TCTCCCTTCT ACAACACACC TCCTTTGTTT
    TCTACTGCTG GCAAGAAAGA TACAAATAAT ATGCCAACAG ACCCTGAGAA CAGTGTCATT
    TACAAGAACA GAGAGAAAAA TGGCTTATAT TATTCTTCTG TTGTCTCTAC TCCATCCTTT
    AAGAATAACC CAGAAATGCT ATGTGAAGAA AATTATTTTT GCTCACCTGT CTATTCTGTA
    AATAAGCATT CAGATTCTTT ACTATCCAAT GAGGCCATAA AAAAAGATGG TTTGACATCT
    CACTGTTTAG GAGTTTCAGA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTAAAG
    TCCAAATCAA CTAGTATGGG TAAAGAACTA GATTCTGGGA TTACAGAACA TTTCCCTCAG
    ATACAACTAC AAGAAAGTGT AAACATTCCT TCTAAACCAA AGTGCATAAT TGAACAAGAC
    AAGTGTGGTG AGGTGAAGAG CACAGACAAT TTATACTACC AGCATCAATC GGAAAATACT
    ATGAGCTATA AAAGCAGATG GGCCATGTAT TTATCCTCAC AGAGTTCACC TGTACGTTCT
    TCTACTGAAG ATGGAAATAA TATAGAAAGG AAACCCAAAA CTCAGGGAGA TAATGTAAAT
    TTAAATTTGA AAGACCTTGT ACAAAAAAGG ATACAGTTTT TTGAAACATG TACTGAAAAG
    GGTAAAAACT ATAATGAAGA CAAGCCAGTA GGTGATAATG ATCAATCTTG CGATCAGGAA
    GTAAAATTGG AAATTCCTTC ATTCTGTAAA AGCAACAGCT TACCATTTAC CTGTAGCAGT
    GTAGAGAATG ATGGCTTATT ATCAGAATCT GACATTCAAA AAAATAATAA AATACCTGTT
    AGTCAGGATG ACCAGATGTG TCTAAAAGGA TCAATTCTCA TTAGAGAAAA TGCTCAGAAG
    ATAAATATAT GTGGAACCCC AGAAAAGTAT GACCAACCCA TGTCGTCCCT GCCAGAATCT
    GAACATCTAC AAATTGAGTC TTCTACAAGT AACAATTCTA GGATCTCTGA TGACTTTACC
    AATATGCTTT CTAAAAGTAA TACTGATAAT GAAAATCTTA ATAGTATTCC TGAACCAATG
    AGTAATGTAA CACAGCCACT TGTGGAGGTG ACTTTTAATC TGAACAATTT TGAAACCAGT
    GACTCTGAGG AGGAATCACA AGAAAATAAC AAAATTTCCC AGGATTCAGA GGGTTGGGTA
    AAGGAAACTC TGGTTAATGA TGGCAGTTCA AGTGTTCAAA AGAGATGTGA AAATATAAGC
    TGTAAGAAAG TTGGCAGTGA ACATTTGTCT CATTTAACAT CTATGGAGGA TAAGCCTACA
    GAGACATTTC CTAAAGAGAC TCTGCTGTCA CAGTTTTGTG ATAAAGCGTG TATGGGTTTT
    GACACAGGAC CTTGGAAAAC TGGAAACAGA AAAGAAATAG AAGAGTATAA TGACACATTA
    AGCAGTTTTG ATTCATCTTT AGAGTGGACT GATGATGTAT ATGTAGATAA TAATGAAGAT
    GCTAATAAAT CTATACAAAA AGTCAGAATT AACTATGATA TTGCTTCACT ACCGAGTAAG
    TCTAAAGGTA TAAGCTCAAA TTTACATATT CCTCAGTTAA ACATAGCCAC TAATCAGATT
    CCTAAAAACA GCCTATTCTC GGAACAGTCT GGGCTTAAGC CTTTTATAAT GGGAAATAAA
    TTAGACAAAA ATGATGAACA GGTTTTACTG TCAACTTCTA GCAGTGACAT CAGTATCCAA
    CAATTAAATG TTAATCAGAA TCACTCTGAA GAACCTGTTG CTCTTGGTAA ATCAAATACC
    CAAGTTTCCA ATTCTTTGTT GTACCTTCTC GGAGAAAAGC ATCCTATTTC CAAAGAAACA
    GAAGCGTGTA TTCCTGAATC CGAAGATTTG GGAAGGATTA GAAGTTTAGA CCATGACCAT
    ATTGAAGTAG AACCTGCTGG AGAAAGCAAA CAACATTGGA ATAGTCCTAG GAATCCTTCA
    GAACTCTCTG GATTAATAAA TAACATTTCC CTTTTAAAGT CATTGTCTGA ACATAGTACA
    GCTTTAGAAG GCTTGGAAAT ATTGAAAAAG AAAAATACTA CCTTTAAACA ACAAGGAACT
    CTACAGACAT ATAATCCAGA TAGTAATTCT GAAGCTAGGA AACGATCGAC TGCAGAAGTT
    TTGCAAGCTT TACCAAAGTT GCCTCCTCTG AACCAGGATT CTCAACAGAT TATAAAAGAA
    AATGAAGTTG AACCAAGTAA ACCACTTCAG TCTTTGCAGC CTGTTGAGTT TCAAGGGCAC
    CAAGTGAAAG GATCTGCTAC TAGTGCTGTA ATGATGAGAG GACACAGCTC ACAGCTAGAA
    TGCGGTCAAT TTCCAGATAG TATTGAGTAT GAAAACTGCA CAAGAGACAC TTGTTTATTG
    ACACCAAAAT TGACCAGTGC TTGTATGCAG ATTGATTTCC TGCAGTTCCC AGATGAAAGA
    AACATTCCAA AAGAAGATAT TAGCTTTAAT ATTGAAGACA ATTTTCAAGT GAGAGCTGAG
    TCAAATAAGG ACTCAATTGA AAATGACTTG ATTTTGGATT TCTCTCCTAA AAATTCAGAA
    CATTTCTATC ATTATTTGGA TTCTAGTTCT AAATCAGCAG AGAAGACTTC ATGGGAAGCA
    AATAAGGTAA CATCACCAGA ACAGAAGATC TCTACATTAA ACCCTGATTC TACTTTTTCT
    TTGAACTCAC GAGATGAAGA CTTTGTGCTA GAATTCTCTG AGGAGTCCCT GAAAGAAAGA
    ACTTTACCTG TTATCAAAAA ATCCAGTTCT TTGGAGAATA AGAACTTTCA AAGGCTTCCA
    TTAGTAAGTA GAACCCGAGT TCCACTAACT ACTTTGCCAC CTGCTGATTC AGATTCTTGT
    TCATACGTGA TCAACTGTGA CAGGCAAGAA TATTCTGGTT CCTCCCCCAT ATTCAACTTA
    TGTGAAGAAT CAGGAGTTCT TTCATTTAGC TTGGGGCTTG AGGACCAATG TGAAACTTCC
    TTCTGTAAAG AATCTAGAGA AGTGATTCCA CAGGACGTTT TGCTTCTCAA TAATATACCT
    ACTCAAAGCA AGTGGCTGAA ATATCAGAAC ATACCCCAGT GCAACTTGAC TACTCCAGAC
    AGAGTTGATA AGAAAGTAAC AGATGGTTTC TCTGGTAAAG TTATTTCTGG GACACATTTA
    AGTGATATAC GTGAAAGACA GAGTGGTGCT GTGAATGAAA GCTCTTTAGA CTCTATATAT
    TTGCAAATGA TAAAAGGCAT GCTCTATCAA CAGCAGCAGG ATTTTAGCTG TCAGGATTTG
    GTTTTCAGAA AGAAAGCACT CTCATTGAAT TTAAAGCTGA CTTCCCAGAC TGAAAAAATT
    CAAAACACGT TAAGAGAGTC TGCTGCCTGC TACATCTACA GTGTAACAGG AGATTTACAG
    GAGATAAATG CCTCTGAGCT ATGCTTCCCA AGTGGGCAGA AAATAAAATC TGCTTATCTT
    CCCCAAAGGC AGATTCATAT ACCAGCTGTT TTTCAGTCTC CTGCTCATTA TAAGCAGATT
    TTTACATCTT GTCTCATAGA ACACTTAAAT ATATTGTTGT TTGGGTTGGC ACAGAGGTTG
    CACAAAGCTC TTTCAAAAGT TGACATATCA TTTTACACAT CATTAAAGGG AGAGAAACTA
    AAAAATGTAG AAAGTAATGT ACCATCCTGC CATCATCATC AACCTGCAAA ACTTGTCATG
    GTTAAAAAGG AAGGTCCAAA TAAGGGTCGT CTTTTTTATA CCTGTGATGG ACCCAAAGCT
    GACCGATGTA AATTTTTCAA GTGGCTTGAA GAAGTGACCC CAGGATATTT GACACAAGAA
    GAGTCTCTAC CTAGCATAGT TTTAAGTGAT ATAAAGAGTA TTGGCTTATA CTTAAGAAGT
    CAAAATATAC CCCTCTTTGA GGAATGTCAG CTTTTGGTGA GGAAAGGATT TGATTTTCAA
    AGAAAACAGT ATGGCAAACT GAAGAAGTTT AATGCTGTAA ATCCTGAATT TTATAGTGAA
    CCTAAAAGCA AACTTTATCT TAAGCTGAGT CGGAGGGAAA GTTCTTCAAC TTACAGCAAA
    AATGATCTTT GGGTGGTTTC AAAAACATTG GACTTTGAAT TGGATACTTT TATTGCGTGT
    AGTGCTTTCT TTGGACCATC ATCTATCAAT GAGGTGGAGC TACTACCTTT GAAAGGTTAT
    TTCCCCTCTA ACTGGCCCAC TAACATAGTG GTACATGCAT TGTTGGTTTG TAATGCTAGC
    ACTGAGCTGA CAACTTTGAA AAACATTCAG GACTACTTTA ATCCAGCTAC TCTACCTCTA
    ACACAGTACT TGTTAACAAT GTCTTCATCA ACTATAGTTA GCAACAAGAG AGTCAATAAG
    AGAAAATTTA TCCCACCAGC CTTCTCAAAT ATCAATACAA AATTTGAACT TCTCAGCCTA
    GAAGCCACAT TACAGTTAGC TAGTGAATTA ATTCAGGCAC ACAACTTAAA TAAGGATCAA
    GCTACAGCTT TAATTCAGAT AGCTCAAATG ATGGCATCAC ATGAAAATGC TGAAGAAGTG
    AAGAAAGTAC AAACCCATAC CCTCCCTATC ACAATCATAC ATGGTGTTTT TGGAGCAGGA
    AAGAGTTATT TGTTGGCAGT GGTGATTTTG TTTTTTGTAC AGCTATTTGA AAAGAGTGAA
    GTTCTCACAG TTGGAAATGT AAGACCTTGG AAACTTCTCA TTTCTTCTTC CACTAATGTA
    GCTGTGGACA GAGTACTTCT TGGGCTTCTC AGTCTTGGTT TTGAAAAGTT CGTCAGAGTT
    GGGAGTGTCA GGAAGATTGC CAAGCCAATT TTACCTTATA GTTTGCATGC TGGCTCAGAA
    AATGAAAATG AACAACTAAA AGAACTACAT GGACTAATGA AAGAAGACTT GACTCCTCTG
    GAAAGGGTCT ATGTCAGAAA AAGTATTGAG CAGCATAAAC TGGGGACCAA TAAAACCCTG
    CTGAAGCAGG TACGAGTAGT TGGAGTTACC TGTGCAGCTT GCCCATTCCC ATGTATGAAT
    GATCTTAAAT TTCCTGTAGT TGTGTTGGAT GAGTGTAGTC AAATAACTGA ACCGGCCTCT
    CTCCTTCCTA TTGCAAGGTT TGAGTGTGAA AAGCTGATTC TTGTTGGAGA TCCTAAACAG
    CTTTCTCCTA CTATTCAGGG TTCTGATGCA GCCCATGAAA ATGGATTGGA ACAAACTCTT
    TTTGATCGGC TTTGCTTAAT GGGCCATAAG CCAGTTCTGT TGAGAACCCA GTACCGTTGT
    CACCCTGCAA TCAGTGATAT CTCTAATGAT CTGTTTTATG AAGGAAACCT TGTGGATGGC
    ATTTCTGAAA CAGAGAGGGG CCCTTTATTG GAATGGCTCC CAACACTATG TTTCTATAAC
    ATTAAAGGAC CTGAACAGAT TGAAAGAGAT AACAGCTTTC ATAATGTGGC AGAAGCTGCT
    TTTACACTCA AGCTGATTCA ATCACTGATT GCGAGTGGGA TAGCGGGCTC TATGATTGGG
    GTGATAACAT TATACAAATC CCAGATGTAC AAGCTTTGTC ATTTACTCAG TGCCGTGGAC
    TTTGACAATC CTGATATTAA AGCTGTGCAG GTATCCACAG TAGATGCTTT TCAGGGAGCT
    GAAAAGGAGA TCATTATTCT GTCCTGTGTA AGGACAAGAC AAGTAGGATT TATTGATTCA
    GAAAAAAGAA TGAATGTTGC ACTGACCAGA GGAAGGAGGC ATTTATTGAT CGTGGGAAAT
    TTAGCCTGTT TGCGGAAAAA TCGACTCTGG GGACGTGTAA TCCAACACTG TGAAGGAAGG
    AGAGATGGAT TACAACATGC AAGCCAGTGT GAACCACAGC TGAACCATCT TCTTAAAGAG
    TATTTTGAAA AACGAGCAGA AGAAAAGCAG AAAAAAAGTG AAAAAGATAA ATCTAAAGAT
    AAATCACATT CACAAAAAGA TATGGTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269121.1
    CDS316..6765
    Translation

    Target ORF information:

    RefSeq Version XM_022413413.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris zinc finger GRF-type containing 1 (ZGRF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413413.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGAAAGCC AGGAATTTAT TGTACTGTAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
    TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAGTTTT ATATGATGAC
    AAAGGAGAAT GTTTGGAGAG TATATTTCTT AAGTGCCTTG AGGTGAAGCC TGGAGATGAC
    TTAGAAGGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATTGCT
    ATTAAACCGG ATGTCATTAA AGAAACATCA GAGTTAAATC CAAGGAAATT TATATCCTCT
    GGGCGGTCCC TTGGATGTCA GCCTGCTGGT TTAAAAAGGA AGTTTACTGG TTTTCAAGGA
    CCACGTCAAG TGCCAAGGAA AATGGTTATT ACAGAAAATG ATGAATCAGC TGCATCACCT
    GAGGCTAAGA AACCTGGCCC TATTTTTCTT TCTCCCTTCT ACAACACACC TCCTTTGTTT
    TCTACTGCTG GCAAGAAAGA TACAAATAAT ATGCCAACAG ACCCTGAGAA CAGTGTCATT
    TACAAGAACA GAGAGAAAAA TGGCTTATAT TATTCTTCTG TTGTCTCTAC TCCATCCTTT
    AAGAATAACC CAGAAATGCT ATGTGAAGAA AATTATTTTT GCTCACCTGT CTATTCTGTA
    AATAAGCATT CAGATTCTTT ACTATCCAAT GAGGCCATAA AAAAAGATGG TTTGACATCT
    CACTGTTTAG GAGTTTCAGA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTAAAG
    TCCAAATCAA CTAGTATGGG TAAAGAACTA GATTCTGGGA TTACAGAACA TTTCCCTCAG
    ATACAACTAC AAGAAAGTGT AAACATTCCT TCTAAACCAA AGTGCATAAT TGAACAAGAC
    AAGTGTGGTG AGGTGAAGAG CACAGACAAT TTATACTACC AGCATCAATC GGAAAATACT
    ATGAGCTATA AAAGCAGATG GGCCATGTAT TTATCCTCAC AGAGTTCACC TGTACGTTCT
    TCTACTGAAG ATGGAAATAA TATAGAAAGG AAACCCAAAA CTCAGGGAGA TAATGTAAAT
    TTAAATTTGA AAGACCTTGT ACAAAAAAGG ATACAGTTTT TTGAAACATG TACTGAAAAG
    GGTAAAAACT ATAATGAAGA CAAGCCAGTA GGTGATAATG ATCAATCTTG CGATCAGGAA
    GTAAAATTGG AAATTCCTTC ATTCTGTAAA AGCAACAGCT TACCATTTAC CTGTAGCAGT
    GTAGAGAATG ATGGCTTATT ATCAGAATCT GACATTCAAA AAAATAATAA AATACCTGTT
    AGTCAGGATG ACCAGATGTG TCTAAAAGGA TCAATTCTCA TTAGAGAAAA TGCTCAGAAG
    ATAAATATAT GTGGAACCCC AGAAAAGTAT GACCAACCCA TGTCGTCCCT GCCAGAATCT
    GAACATCTAC AAATTGAGTC TTCTACAAGT AACAATTCTA GGATCTCTGA TGACTTTACC
    AATATGCTTT CTAAAAGTAA TACTGATAAT GAAAATCTTA ATAGTATTCC TGAACCAATG
    AGTAATGTAA CACAGCCACT TGTGGAGGTG ACTTTTAATC TGAACAATTT TGAAACCAGT
    GACTCTGAGG AGGAATCACA AGAAAATAAC AAAATTTCCC AGGATTCAGA GGGTTGGGTA
    AAGGAAACTC TGGTTAATGA TGGCAGTTCA AGTGTTCAAA AGAGATGTGA AAATATAAGC
    TGTAAGAAAG TTGGCAGTGA ACATTTGTCT CATTTAACAT CTATGGAGGA TAAGCCTACA
    GAGACATTTC CTAAAGAGAC TCTGCTGTCA CAGTTTTGTG ATAAAGCGTG TATGGGTTTT
    GACACAGGAC CTTGGAAAAC TGGAAACAGA AAAGAAATAG AAGAGTATAA TGACACATTA
    AGCAGTTTTG ATTCATCTTT AGAGTGGACT GATGATGTAT ATGTAGATAA TAATGAAGAT
    GCTAATAAAT CTATACAAAA AGTCAGAATT AACTATGATA TTGCTTCACT ACCGAGTAAG
    TCTAAAGGTA TAAGCTCAAA TTTACATATT CCTCAGTTAA ACATAGCCAC TAATCAGATT
    CCTAAAAACA GCCTATTCTC GGAACAGTCT GGGCTTAAGC CTTTTATAAT GGGAAATAAA
    TTAGACAAAA ATGATGAACA GGTTTTACTG TCAACTTCTA GCAGTGACAT CAGTATCCAA
    CAATTAAATG TTAATCAGAA TCACTCTGAA GAACCTGTTG CTCTTGGTAA ATCAAATACC
    CAAGTTTCCA ATTCTTTGTT GTACCTTCTC GGAGAAAAGC ATCCTATTTC CAAAGAAACA
    GAAGCGTGTA TTCCTGAATC CGAAGATTTG GGAAGGATTA GAAGTTTAGA CCATGACCAT
    ATTGAAGTAG AACCTGCTGG AGAAAGCAAA CAACATTGGA ATAGTCCTAG GAATCCTTCA
    GAACTCTCTG GATTAATAAA TAACATTTCC CTTTTAAAGT CATTGTCTGA ACATAGTACA
    GCTTTAGAAG GCTTGGAAAT ATTGAAAAAG AAAAATACTA CCTTTAAACA ACAAGGAACT
    CTACAGACAT ATAATCCAGA TAGTAATTCT GAAGCTAGGA AACGATCGAC TGCAGAAGTT
    TTGCAAGCTT TACCAAAGTT GCCTCCTCTG AACCAGGATT CTCAACAGAT TATAAAAGAA
    AATGAAGTTG AACCAAGTAA ACCACTTCAG TCTTTGCAGC CTGTTGAGTT TCAAGGGCAC
    CAAGTGAAAG GATCTGCTAC TAGTGCTGTA ATGATGAGAG GACACAGCTC ACAGCTAGAA
    TGCGGTCAAT TTCCAGATAG TATTGAGTAT GAAAACTGCA CAAGAGACAC TTGTTTATTG
    ACACCAAAAT TGACCAGTGC TTGTATGCAG ATTGATTTCC TGCAGTTCCC AGATGAAAGA
    AACATTCCAA AAGAAGATAT TAGCTTTAAT ATTGAAGACA ATTTTCAAGT GAGAGCTGAG
    TCAAATAAGG ACTCAATTGA AAATGACTTG ATTTTGGATT TCTCTCCTAA AAATTCAGAA
    CATTTCTATC ATTATTTGGA TTCTAGTTCT AAATCAGCAG AGAAGACTTC ATGGGAAGCA
    AATAAGGTAA CATCACCAGA ACAGAAGATC TCTACATTAA ACCCTGATTC TACTTTTTCT
    TTGAACTCAC GAGATGAAGA CTTTGTGCTA GAATTCTCTG AGGAGTCCCT GAAAGAAAGA
    ACTTTACCTG TTATCAAAAA ATCCAGTTCT TTGGAGAATA AGAACTTTCA AAGGCTTCCA
    TTAGTAAGTA GAACCCGAGT TCCACTAACT ACTTTGCCAC CTGCTGATTC AGATTCTTGT
    TCATACGTGA TCAACTGTGA CAGGCAAGAA TATTCTGGTT CCTCCCCCAT ATTCAACTTA
    TGTGAAGAAT CAGGAGTTCT TTCATTTAGC TTGGGGCTTG AGGACCAATG TGAAACTTCC
    TTCTGTAAAG AATCTAGAGA AGTGATTCCA CAGGACGTTT TGCTTCTCAA TAATATACCT
    ACTCAAAGCA AGTGGCTGAA ATATCAGAAC ATACCCCAGT GCAACTTGAC TACTCCAGAC
    AGAGTTGATA AGAAAGTAAC AGATGGTTTC TCTGGTAAAG TTATTTCTGG GACACATTTA
    AGTGATATAC GTGAAAGACA GAGTGGTGCT GTGAATGAAA GCTCTTTAGA CTCTATATAT
    TTGCAAATGA TAAAAGGCAT GCTCTATCAA CAGCAGCAGG ATTTTAGCTG TCAGGATTTG
    GTTTTCAGAA AGAAAGCACT CTCATTGAAT TTAAAGCTGA CTTCCCAGAC TGAAAAAATT
    CAAAACACGT TAAGAGAGTC TGCTGCCTGC TACATCTACA GTGTAACAGG AGATTTACAG
    GAGATAAATG CCTCTGAGCT ATGCTTCCCA AGTGGGCAGA AAATAAAATC TGCTTATCTT
    CCCCAAAGGC AGATTCATAT ACCAGCTGTT TTTCAGTCTC CTGCTCATTA TAAGCAGATT
    TTTACATCTT GTCTCATAGA ACACTTAAAT ATATTGTTGT TTGGGTTGGC ACAGAGGTTG
    CACAAAGCTC TTTCAAAAGT TGACATATCA TTTTACACAT CATTAAAGGG AGAGAAACTA
    AAAAATGTAG AAAGTAATGT ACCATCCTGC CATCATCATC AACCTGCAAA ACTTGTCATG
    GTTAAAAAGG AAGGTCCAAA TAAGGGTCGT CTTTTTTATA CCTGTGATGG ACCCAAAGCT
    GACCGATGTA AATTTTTCAA GTGGCTTGAA GAAGTGACCC CAGGATATTT GACACAAGAA
    GAGTCTCTAC CTAGCATAGT TTTAAGTGAT ATAAAGAGTA TTGGCTTATA CTTAAGAAGT
    CAAAATATAC CCCTCTTTGA GGAATGTCAG CTTTTGGTGA GGAAAGGATT TGATTTTCAA
    AGAAAACAGT ATGGCAAACT GAAGAAGTTT AATGCTGTAA ATCCTGAATT TTATAGTGAA
    CCTAAAAGCA AACTTTATCT TAAGCTGAGT CGGAGGGAAA GTTCTTCAAC TTACAGCAAA
    AATGATCTTT GGGTGGTTTC AAAAACATTG GACTTTGAAT TGGATACTTT TATTGCGTGT
    AGTGCTTTCT TTGGACCATC ATCTATCAAT GAGGTGGAGC TACTACCTTT GAAAGGTTAT
    TTCCCCTCTA ACTGGCCCAC TAACATAGTG GTACATGCAT TGTTGGTTTG TAATGCTAGC
    ACTGAGCTGA CAACTTTGAA AAACATTCAG GACTACTTTA ATCCAGCTAC TCTACCTCTA
    ACACAGTACT TGTTAACAAT GTCTTCATCA ACTATAGTTA GCAACAAGAG AGTCAATAAG
    AGAAAATTTA TCCCACCAGC CTTCTCAAAT ATCAATACAA AATTTGAACT TCTCAGCCTA
    GAAGCCACAT TACAGTTAGC TAGTGAATTA ATTCAGGCAC ACAACTTAAA TAAGGATCAA
    GCTACAGCTT TAATTCAGAT AGCTCAAATG ATGGCATCAC ATGAAAATGC TGAAGAAGTG
    AAGAAAGTAC AAACCCATAC CCTCCCTATC ACAATCATAC ATGGTGTTTT TGGAGCAGGA
    AAGAGTTATT TGTTGGCAGT GGTGATTTTG TTTTTTGTAC AGCTATTTGA AAAGAGTGAA
    GTTCTCACAG TTGGAAATGT AAGACCTTGG AAACTTCTCA TTTCTTCTTC CACTAATGTA
    GCTGTGGACA GAGTACTTCT TGGGCTTCTC AGTCTTGGTT TTGAAAAGTT CGTCAGAGTT
    GGGAGTGTCA GGAAGATTGC CAAGCCAATT TTACCTTATA GTTTGCATGC TGGCTCAGAA
    AATGAAAATG AACAACTAAA AGAACTACAT GGACTAATGA AAGAAGACTT GACTCCTCTG
    GAAAGGGTCT ATGTCAGAAA AAGTATTGAG CAGCATAAAC TGGGGACCAA TAAAACCCTG
    CTGAAGCAGG TACGAGTAGT TGGAGTTACC TGTGCAGCTT GCCCATTCCC ATGTATGAAT
    GATCTTAAAT TTCCTGTAGT TGTGTTGGAT GAGTGTAGTC AAATAACTGA ACCGGCCTCT
    CTCCTTCCTA TTGCAAGGTT TGAGTGTGAA AAGCTGATTC TTGTTGGAGA TCCTAAACAG
    CTTTCTCCTA CTATTCAGGG TTCTGATGCA GCCCATGAAA ATGGATTGGA ACAAACTCTT
    TTTGATCGGC TTTGCTTAAT GGGCCATAAG CCAGTTCTGT TGAGAACCCA GTACCGTTGT
    CACCCTGCAA TCAGTGATAT CTCTAATGAT CTGTTTTATG AAGGAAACCT TGTGGATGGC
    ATTTCTGAAA CAGAGAGGGG CCCTTTATTG GAATGGCTCC CAACACTATG TTTCTATAAC
    ATTAAAGGAC CTGAACAGAT TGAAAGAGAT AACAGCTTTC ATAATGTGGC AGAAGCTGCT
    TTTACACTCA AGCTGATTCA ATCACTGATT GCGAGTGGGA TAGCGGGCTC TATGATTGGG
    GTGATAACAT TATACAAATC CCAGATGTAC AAGCTTTGTC ATTTACTCAG TGCCGTGGAC
    TTTGACAATC CTGATATTAA AGCTGTGCAG GTATCCACAG TAGATGCTTT TCAGGGAGCT
    GAAAAGGAGA TCATTATTCT GTCCTGTGTA AGGACAAGAC AAGTAGGATT TATTGATTCA
    GAAAAAAGAA TGAATGTTGC ACTGACCAGA GGAAGGAGGC ATTTATTGAT CGTGGGAAAT
    TTAGCCTGTT TGCGGAAAAA TCGACTCTGG GGACGTGTAA TCCAACACTG TGAAGGAAGG
    AGAGATGGAT TACAACATGC AAGCCAGTGT GAACCACAGC TGAACCATCT TCTTAAAGAG
    TATTTTGAAA AACGAGCAGA AGAAAAGCAG AAAAAAAGTG AAAAAGATAA ATCTAAAGAT
    AAATCACATT CACAAAAAGA TATGGTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284013
    Clone ID Related Accession (Same CDS sequence) XM_022413414.1
    Accession Version XM_022413414.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6342bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein ZGRF1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGAAAGCC AGGAATTTAT TGTACTGTAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
    TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAGTTTT ATATGATGAC
    AAAGGAGAAT GTTTGGAGAG TATATTTCTT AAGTGCCTTG AGGTGAAGCC TGGAGATGAC
    TTAGAAGGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATTGCT
    ATTAAACCGG ATGTCATTAA AGAAACATCA GAGTTAAATC CAAGGAAATT TATATCCTCT
    GGGCGGTCCC TTGGATGTCA GCCTGCTGGT TTAAAAAGGA AGTTTACTGG TTTTCAAGGA
    CCACGTCAAG TGCCAAGGAA AATGGTTATT ACAGAAAATG ATGAATCAGC TGCATCACCT
    GAGGCTAAGA AACCTGGCCC TATTTTTCTT TCTCCCTTCT ACAACACACC TCCTTTGTTT
    TCTACTGCTG GCAAGAAAGA TACAAATAAT ATGCCAACAG ACCCTGAGAA CAGTGTCATT
    TACAAGAACA GAGAGAAAAA TGGCTTATAT TATTCTTCTG TTGTCTCTAC TCCATCCTTT
    AAGAATAACC CAGAAATGCT ATGTGAAGAA AATTATTTTT GCTCACCTGT CTATTCTGTA
    AATAAGCATT CAGATTCTTT ACTATCCAAT GAGGCCATAA AAAAAGATGG TTTGACATCT
    CACTGTTTAG GAGTTTCAGA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTAAAG
    TCCAAATCAA CTAGTATGGG TAAAGAACTA GATTCTGGGA TTACAGAACA TTTCCCTCAG
    ATACAACTAC AAGAAAGTGT AAACATTCCT TCTAAACCAA AGTGCATAAT TGAACAAGAC
    AAGTGTGGTG AGGTGAAGAG CACAGACAAT TTATACTACC AGCATCAATC GGAAAATACT
    ATGAGCTATA AAAGCAGATG GGCCATGTAT TTATCCTCAC AGAGTTCACC TGTACGTTCT
    TCTACTGAAG ATGGAAATAA TATAGAAAGG AAACCCAAAA CTCAGGGAGA TAATGTAAAT
    TTAAATTTGA AAGACCTTGT ACAAAAAAGG ATACAGTTTT TTGAAACATG TACTGAAAAG
    GGTAAAAACT ATAATGAAGA CAAGCCAGTA GGTGATAATG ATCAATCTTG CGATCAGGAA
    GTAAAATTGG AAATTCCTTC ATTCTGTAAA AGCAACAGCT TACCATTTAC CTGTAGCAGT
    GTAGAGAATG ATGGCTTATT ATCAGAATCT GACATTCAAA AAAATAATAA AATACCTGTT
    AGTCAGGATG ACCAGATGTG TCTAAAAGGA TCAATTCTCA TTAGAGAAAA TGCTCAGAAG
    ATAAATATAT GTGGAACCCC AGAAAAGTAT GACCAACCCA TGTCGTCCCT GCCAGAATCT
    GAACATCTAC AAATTGAGTC TTCTACAAGT AACAATTCTA GGATCTCTGA TGACTTTACC
    AATATGCTTT CTAAAAGTAA TACTGATAAT GAAAATCTTA ATAGTATTCC TGAACCAATG
    AGTAATGTAA CACAGCCACT TGTGGAGGTG ACTTTTAATC TGAACAATTT TGAAACCAGT
    GACTCTGAGG AGGAATCACA AGAAAATAAC AAAATTTCCC AGGATTCAGA GGGTTGGGTA
    AAGGAAACTC TGGTTAATGA TGGCAGTTCA AGTGTTCAAA AGAGATGTGA AAATATAAGC
    TGTAAGAAAG TTGGCAGTGA ACATTTGTCT CATTTAACAT CTATGGAGGA TAAGCCTACA
    GAGACATTTC CTAAAGAGAC TCTGCTGTCA CAGTTTTGTG ATAAAGCGTG TATGGGTTTT
    GACACAGGAC CTTGGAAAAC TGGAAACAGA AAAGAAATAG AAGAGTATAA TGACACATTA
    AGCAGTTTTG ATTCATCTTT AGAGTGGACT GATGATGTAT ATGTAGATAA TAATGAAGAT
    GCTAATAAAT CTATACAAAA AGTCAGAATT AACTATGATA TTGCTTCACT ACCGAGTAAG
    TCTAAAGGTA TAAGCTCAAA TTTACATATT CCTCAGTTAA ACATAGCCAC TAATCAGATT
    CCTAAAAACA GCCTATTCTC GGAACAGTCT GGGCTTAAGC CTTTTATAAT GGGAAATAAA
    TTAGACAAAA ATGATGAACA GGTTTTACTG TCAACTTCTA GCAGTGACAT CAGTATCCAA
    CAATTAAATG TTAATCAGAA TCACTCTGAA GAACCTGTTG CTCTTGGTAA ATCAAATACC
    CAAGTTTCCA ATTCTTTGTT GTACCTTCTC GGAGAAAAGC ATCCTATTTC CAAAGAAACA
    GAAGCGTGTA TTCCTGAATC CGAAGATTTG GGAAGGATTA GAAGTTTAGA CCATGACCAT
    ATTGAAGTAG AACCTGCTGG AGAAAGCAAA CAACATTGGA ATAGTCCTAG GAATCCTTCA
    GAACTCTCTG GATTAATAAA TAACATTTCC CTTTTAAAGT CATTGTCTGA ACATAGTACA
    GCTTTAGAAG GCTTGGAAAT ATTGAAAAAG AAAAATACTA CCTTTAAACA ACAAGGAACT
    CTACAGACAT ATAATCCAGA TAGTAATTCT GAAGCTAGGA AACGATCGAC TGCAGAAGTT
    TTGCAAGCTT TACCAAAGTT GCCTCCTCTG AACCAGGATT CTCAACAGAT TATAAAAGAA
    AATGAAGTTG AACCAAGTAA ACCACTTCAG TCTTTGCAGT TCTTTTCCTT TGGAGATAAA
    GAGACTGCTT TTCTAGATGT TATGCCTAAA CAATCAGAGA GAAAAACCTG TGACCCTAAG
    CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA GGATCTGCTA CTAGTGCTGT AATGATGAGA
    GGACACAGCT CACAGCTAGA ATGCGGTCAA TTTCCAGATA GTATTGAGTA TGAAAACTGC
    ACAAGAGACA CTTGTTTATT GACACCAAAA TTGACCAGTG CTTGTATGCA GATTGATTTC
    CTGCAGTTCC CAGATGAAAG AAACATTCCA AAAGAAGATA TTAGCTTTAA TATTGAAGAC
    AATTTTCAAG TGAGAGCTGA GTCAAATAAG GACTCAATTG AAAATGACTT GATTTTGGAT
    TTCTCTCCTA AAAATTCAGA ACATTTCTAT CATTATTTGG ATTCTAGTTC TAAATCAGCA
    GAGAAGACTT CATGGGAAGC AAATAAGGTA ACATCACCAG AACAGAAGAT CTCTACATTA
    AACCCTGATT CTACTTTTTC TTTGAACTCA CGAGATGAAG ACTTTGTGCT AGAATTCTCT
    GAGGAGTCCC TGAAAGAAAG AACTTTACCT GTTATCAAAA AATCCAGTTC TTTGGAGAAT
    AAGAACTTTC AAAGGCTTCC ATTAGTAAGT AGAACCCGAG TTCCACTAAC TACTTTGCCA
    CCTGCTGATT CAGATTCTTG TTCATACGTG ATCAACTGTG ACAGGCAAGA ATATTCTGGT
    TCCTCCCCCA TATTCAACTT ATGTGAAGAA TCAGGAGTTC TTTCATTTAG CTTGGGGCTT
    GAGGACCAAT GTGAAACTTC CTTCTGTAAA GAATCTAGAG AAGTGATTCC ACAGGACGTT
    TTGCTTCTCA ATAATATACC TACTCAAAGC AAGTGGCTGA AATATCAGAA CATACCCCAG
    TGCAACTTGA CTACTCCAGA CAGAGTTGAT AAGAAAGTAA CAGATGGTTT CTCTGGTAAA
    GTTATTTCTG GGACACATTT AAGTGATATA CGTGAAAGAC AGAGTGGTGC TGTGAATGAA
    AGCTCTTTAG ACTCTATATA TTTGCAAATG ATAAAAGGCA TGCTCTATCA ACAGCAGCAG
    GATTTTAGCT GTCAGGATTT GGTTTTCAGA AAGAAAGCAC TCTCATTGAA TTTAAAGCTG
    ACTTCCCAGA CTGAAAAAAT TCAAAACACG TTAAGAGAGT CTGCTGCCTG CTACATCTAC
    AGTGTAACAG GAGATTTACA GGAGATAAAT GCCTCTGAGC TATGCTTCCC AAGTGGGCAG
    AAAATAAAAT CTGCTTATCT TCCCCAAAGG CAGATTCATA TACCAGCTGT TTTTCAGTCT
    CCTGCTCATT ATAAGCAGAT TTTTACATCT TGTCTCATAG AACACTTAAA TATATTGTTG
    TTTGGGTTGG CACAGAGGTT GCACAAAGCT CTTTCAAAAG TTGACATATC ATTTTACACA
    TCATTAAAGG GAGAGAAACT AAAAAATGTA GAAAGTAATG TACCATCCTG CCATCATCAT
    CAACCTGCAA AACTTGTCAT GGTTAAAAAG GAAGGTCCAA ATAAGGGTCG TCTTTTTTAT
    ACCTGTGATG GACCCAAAGC TGACCGATGT AAATTTTTCA AGTGGCTTGA AGAAGTGACC
    CCAGGATATT TGACACAAGA AGAGTCTCTA CCTAGCATAG TTTTAAGTGA TATAAAGAGT
    ATTGGCTTAT ACTTAAGAAG TCAAAATATA CCCCTCTTTG AGGAATGTCA GCTTTTGGTG
    AGGAAAGGAT TTGATTTTCA AAGAAAACAG TATGGCAAAC TGAAGAAGTT TAATGCTGTA
    AATCCTGAAT TTTATAGTGA ACCTAAAAGC AAACTTTATC TTAAGCTGAG TCGGAGGGAA
    AGTTCTTCAA CTTACAGCAA AAATGATCTT TGGGTGGTTT CAAAAACATT GGACTTTGAA
    TTGGATACTT TTATTGCGTG TAGTGCTTTC TTTGGACCAT CATCTATCAA TGAGGTGGAG
    CTACTACCTT TGAAAGGTTA TTTCCCCTCT AACTGGCCCA CTAACATAGT GGTACATGCA
    TTGTTGGTTT GTAATGCTAG CACTGAGCTG ACAACTTTGA AAAACATTCA GGACTACTTT
    AATCCAGCTA CTCTACCTCT AACACAGTAC TTGTTAACAA TGTCTTCATC AACTATAGTT
    AGCAACAAGA GAGTCAATAA GAGAAAATTT ATCCCACCAG CCTTCTCAAA TATCAATACA
    AAATTTGAAC TTCTCAGCCT AGAAGCCACA TTACAGTTAG CTAGTGAATT AATTCAGGCA
    CACAACTTAA ATAAGGATCA AGCTACAGCT TTAATTCAGA TAGCTCAAAT GATGGCATCA
    CATGAAAATG CTGAAGAAGT GAAGAAAGTA CAAACCCATA CCCTCCCTAT CACAATCATA
    CATGGTGTTT TTGGAGCAGG AAAGAGTTAT TTGTTGGCAG TGGTGATTTT GTTTTTTGTA
    CAGCTATTTG AAAAGAGTGA AGTTCTCACA GTTGGAAATG TAAGACCTTG GAAACTTCTC
    ATTTCTTCTT CCACTAATGT AGCTGTGGAC AGAGTACTTC TTGGGCTTCT CAGTCTTGGT
    TTTGAAAAGT TCGTCAGAGT TGGGAGTGTC AGGAAGATTG CCAAGCCAAT TTTACCTTAT
    AGTTTGCATG CTGGCTCAGA AAATGAAAAT GAACAACTAA AAGAACTACA TGGACTAATG
    AAAGAAGACT TGACTCCTCT GGAAAGGGTC TATGTCAGAA AAAGTATTGA GCAGCATAAA
    CTGGGGACCA ATAAAACCCT GCTGAAGCAG GGTTCTGATG CAGCCCATGA AAATGGATTG
    GAACAAACTC TTTTTGATCG GCTTTGCTTA ATGGGCCATA AGCCAGTTCT GTTGAGAACC
    CAGTACCGTT GTCACCCTGC AATCAGTGAT ATCTCTAATG ATCTGTTTTA TGAAGGAAAC
    CTTGTGGATG GCATTTCTGA AACAGAGAGG GGCCCTTTAT TGGAATGGCT CCCAACACTA
    TGTTTCTATA ACATTAAAGG ACCTGAACAG ATTGAAAGAG ATAACAGCTT TCATAATGTG
    GCAGAAGCTG CTTTTACACT CAAGCTGATT CAATCACTGA TTGCGAGTGG GATAGCGGGC
    TCTATGATTG GGGTGATAAC ATTATACAAA TCCCAGATGT ACAAGCTTTG TCATTTACTC
    AGTGCCGTGG ACTTTGACAA TCCTGATATT AAAGCTGTGC AGGTATCCAC AGTAGATGCT
    TTTCAGGGAG CTGAAAAGGA GATCATTATT CTGTCCTGTG TAAGGACAAG ACAAGTAGGA
    TTTATTGATT CAGAAAAAAG AATGAATGTT GCACTGACCA GAGGAAGGAG GCATTTATTG
    ATCGTGGGAA ATTTAGCCTG TTTGCGGAAA AATCGACTCT GGGGACGTGT AATCCAACAC
    TGTGAAGGAA GGAGAGATGG ATTACAACAT GCAAGCCAGT GTGAACCACA GCTGAACCAT
    CTTCTTAAAG AGTATTTTGA AAAACGAGCA GAAGAAAAGC AGAAAAAAAG TGAAAAAGAT
    AAATCTAAAG ATAAATCACA TTCACAAAAA GATATGGTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269122.1
    CDS318..6659
    Translation

    Target ORF information:

    RefSeq Version XM_022413414.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris zinc finger GRF-type containing 1 (ZGRF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413414.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGAAAGCC AGGAATTTAT TGTACTGTAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
    TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAGTTTT ATATGATGAC
    AAAGGAGAAT GTTTGGAGAG TATATTTCTT AAGTGCCTTG AGGTGAAGCC TGGAGATGAC
    TTAGAAGGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATTGCT
    ATTAAACCGG ATGTCATTAA AGAAACATCA GAGTTAAATC CAAGGAAATT TATATCCTCT
    GGGCGGTCCC TTGGATGTCA GCCTGCTGGT TTAAAAAGGA AGTTTACTGG TTTTCAAGGA
    CCACGTCAAG TGCCAAGGAA AATGGTTATT ACAGAAAATG ATGAATCAGC TGCATCACCT
    GAGGCTAAGA AACCTGGCCC TATTTTTCTT TCTCCCTTCT ACAACACACC TCCTTTGTTT
    TCTACTGCTG GCAAGAAAGA TACAAATAAT ATGCCAACAG ACCCTGAGAA CAGTGTCATT
    TACAAGAACA GAGAGAAAAA TGGCTTATAT TATTCTTCTG TTGTCTCTAC TCCATCCTTT
    AAGAATAACC CAGAAATGCT ATGTGAAGAA AATTATTTTT GCTCACCTGT CTATTCTGTA
    AATAAGCATT CAGATTCTTT ACTATCCAAT GAGGCCATAA AAAAAGATGG TTTGACATCT
    CACTGTTTAG GAGTTTCAGA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTAAAG
    TCCAAATCAA CTAGTATGGG TAAAGAACTA GATTCTGGGA TTACAGAACA TTTCCCTCAG
    ATACAACTAC AAGAAAGTGT AAACATTCCT TCTAAACCAA AGTGCATAAT TGAACAAGAC
    AAGTGTGGTG AGGTGAAGAG CACAGACAAT TTATACTACC AGCATCAATC GGAAAATACT
    ATGAGCTATA AAAGCAGATG GGCCATGTAT TTATCCTCAC AGAGTTCACC TGTACGTTCT
    TCTACTGAAG ATGGAAATAA TATAGAAAGG AAACCCAAAA CTCAGGGAGA TAATGTAAAT
    TTAAATTTGA AAGACCTTGT ACAAAAAAGG ATACAGTTTT TTGAAACATG TACTGAAAAG
    GGTAAAAACT ATAATGAAGA CAAGCCAGTA GGTGATAATG ATCAATCTTG CGATCAGGAA
    GTAAAATTGG AAATTCCTTC ATTCTGTAAA AGCAACAGCT TACCATTTAC CTGTAGCAGT
    GTAGAGAATG ATGGCTTATT ATCAGAATCT GACATTCAAA AAAATAATAA AATACCTGTT
    AGTCAGGATG ACCAGATGTG TCTAAAAGGA TCAATTCTCA TTAGAGAAAA TGCTCAGAAG
    ATAAATATAT GTGGAACCCC AGAAAAGTAT GACCAACCCA TGTCGTCCCT GCCAGAATCT
    GAACATCTAC AAATTGAGTC TTCTACAAGT AACAATTCTA GGATCTCTGA TGACTTTACC
    AATATGCTTT CTAAAAGTAA TACTGATAAT GAAAATCTTA ATAGTATTCC TGAACCAATG
    AGTAATGTAA CACAGCCACT TGTGGAGGTG ACTTTTAATC TGAACAATTT TGAAACCAGT
    GACTCTGAGG AGGAATCACA AGAAAATAAC AAAATTTCCC AGGATTCAGA GGGTTGGGTA
    AAGGAAACTC TGGTTAATGA TGGCAGTTCA AGTGTTCAAA AGAGATGTGA AAATATAAGC
    TGTAAGAAAG TTGGCAGTGA ACATTTGTCT CATTTAACAT CTATGGAGGA TAAGCCTACA
    GAGACATTTC CTAAAGAGAC TCTGCTGTCA CAGTTTTGTG ATAAAGCGTG TATGGGTTTT
    GACACAGGAC CTTGGAAAAC TGGAAACAGA AAAGAAATAG AAGAGTATAA TGACACATTA
    AGCAGTTTTG ATTCATCTTT AGAGTGGACT GATGATGTAT ATGTAGATAA TAATGAAGAT
    GCTAATAAAT CTATACAAAA AGTCAGAATT AACTATGATA TTGCTTCACT ACCGAGTAAG
    TCTAAAGGTA TAAGCTCAAA TTTACATATT CCTCAGTTAA ACATAGCCAC TAATCAGATT
    CCTAAAAACA GCCTATTCTC GGAACAGTCT GGGCTTAAGC CTTTTATAAT GGGAAATAAA
    TTAGACAAAA ATGATGAACA GGTTTTACTG TCAACTTCTA GCAGTGACAT CAGTATCCAA
    CAATTAAATG TTAATCAGAA TCACTCTGAA GAACCTGTTG CTCTTGGTAA ATCAAATACC
    CAAGTTTCCA ATTCTTTGTT GTACCTTCTC GGAGAAAAGC ATCCTATTTC CAAAGAAACA
    GAAGCGTGTA TTCCTGAATC CGAAGATTTG GGAAGGATTA GAAGTTTAGA CCATGACCAT
    ATTGAAGTAG AACCTGCTGG AGAAAGCAAA CAACATTGGA ATAGTCCTAG GAATCCTTCA
    GAACTCTCTG GATTAATAAA TAACATTTCC CTTTTAAAGT CATTGTCTGA ACATAGTACA
    GCTTTAGAAG GCTTGGAAAT ATTGAAAAAG AAAAATACTA CCTTTAAACA ACAAGGAACT
    CTACAGACAT ATAATCCAGA TAGTAATTCT GAAGCTAGGA AACGATCGAC TGCAGAAGTT
    TTGCAAGCTT TACCAAAGTT GCCTCCTCTG AACCAGGATT CTCAACAGAT TATAAAAGAA
    AATGAAGTTG AACCAAGTAA ACCACTTCAG TCTTTGCAGT TCTTTTCCTT TGGAGATAAA
    GAGACTGCTT TTCTAGATGT TATGCCTAAA CAATCAGAGA GAAAAACCTG TGACCCTAAG
    CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA GGATCTGCTA CTAGTGCTGT AATGATGAGA
    GGACACAGCT CACAGCTAGA ATGCGGTCAA TTTCCAGATA GTATTGAGTA TGAAAACTGC
    ACAAGAGACA CTTGTTTATT GACACCAAAA TTGACCAGTG CTTGTATGCA GATTGATTTC
    CTGCAGTTCC CAGATGAAAG AAACATTCCA AAAGAAGATA TTAGCTTTAA TATTGAAGAC
    AATTTTCAAG TGAGAGCTGA GTCAAATAAG GACTCAATTG AAAATGACTT GATTTTGGAT
    TTCTCTCCTA AAAATTCAGA ACATTTCTAT CATTATTTGG ATTCTAGTTC TAAATCAGCA
    GAGAAGACTT CATGGGAAGC AAATAAGGTA ACATCACCAG AACAGAAGAT CTCTACATTA
    AACCCTGATT CTACTTTTTC TTTGAACTCA CGAGATGAAG ACTTTGTGCT AGAATTCTCT
    GAGGAGTCCC TGAAAGAAAG AACTTTACCT GTTATCAAAA AATCCAGTTC TTTGGAGAAT
    AAGAACTTTC AAAGGCTTCC ATTAGTAAGT AGAACCCGAG TTCCACTAAC TACTTTGCCA
    CCTGCTGATT CAGATTCTTG TTCATACGTG ATCAACTGTG ACAGGCAAGA ATATTCTGGT
    TCCTCCCCCA TATTCAACTT ATGTGAAGAA TCAGGAGTTC TTTCATTTAG CTTGGGGCTT
    GAGGACCAAT GTGAAACTTC CTTCTGTAAA GAATCTAGAG AAGTGATTCC ACAGGACGTT
    TTGCTTCTCA ATAATATACC TACTCAAAGC AAGTGGCTGA AATATCAGAA CATACCCCAG
    TGCAACTTGA CTACTCCAGA CAGAGTTGAT AAGAAAGTAA CAGATGGTTT CTCTGGTAAA
    GTTATTTCTG GGACACATTT AAGTGATATA CGTGAAAGAC AGAGTGGTGC TGTGAATGAA
    AGCTCTTTAG ACTCTATATA TTTGCAAATG ATAAAAGGCA TGCTCTATCA ACAGCAGCAG
    GATTTTAGCT GTCAGGATTT GGTTTTCAGA AAGAAAGCAC TCTCATTGAA TTTAAAGCTG
    ACTTCCCAGA CTGAAAAAAT TCAAAACACG TTAAGAGAGT CTGCTGCCTG CTACATCTAC
    AGTGTAACAG GAGATTTACA GGAGATAAAT GCCTCTGAGC TATGCTTCCC AAGTGGGCAG
    AAAATAAAAT CTGCTTATCT TCCCCAAAGG CAGATTCATA TACCAGCTGT TTTTCAGTCT
    CCTGCTCATT ATAAGCAGAT TTTTACATCT TGTCTCATAG AACACTTAAA TATATTGTTG
    TTTGGGTTGG CACAGAGGTT GCACAAAGCT CTTTCAAAAG TTGACATATC ATTTTACACA
    TCATTAAAGG GAGAGAAACT AAAAAATGTA GAAAGTAATG TACCATCCTG CCATCATCAT
    CAACCTGCAA AACTTGTCAT GGTTAAAAAG GAAGGTCCAA ATAAGGGTCG TCTTTTTTAT
    ACCTGTGATG GACCCAAAGC TGACCGATGT AAATTTTTCA AGTGGCTTGA AGAAGTGACC
    CCAGGATATT TGACACAAGA AGAGTCTCTA CCTAGCATAG TTTTAAGTGA TATAAAGAGT
    ATTGGCTTAT ACTTAAGAAG TCAAAATATA CCCCTCTTTG AGGAATGTCA GCTTTTGGTG
    AGGAAAGGAT TTGATTTTCA AAGAAAACAG TATGGCAAAC TGAAGAAGTT TAATGCTGTA
    AATCCTGAAT TTTATAGTGA ACCTAAAAGC AAACTTTATC TTAAGCTGAG TCGGAGGGAA
    AGTTCTTCAA CTTACAGCAA AAATGATCTT TGGGTGGTTT CAAAAACATT GGACTTTGAA
    TTGGATACTT TTATTGCGTG TAGTGCTTTC TTTGGACCAT CATCTATCAA TGAGGTGGAG
    CTACTACCTT TGAAAGGTTA TTTCCCCTCT AACTGGCCCA CTAACATAGT GGTACATGCA
    TTGTTGGTTT GTAATGCTAG CACTGAGCTG ACAACTTTGA AAAACATTCA GGACTACTTT
    AATCCAGCTA CTCTACCTCT AACACAGTAC TTGTTAACAA TGTCTTCATC AACTATAGTT
    AGCAACAAGA GAGTCAATAA GAGAAAATTT ATCCCACCAG CCTTCTCAAA TATCAATACA
    AAATTTGAAC TTCTCAGCCT AGAAGCCACA TTACAGTTAG CTAGTGAATT AATTCAGGCA
    CACAACTTAA ATAAGGATCA AGCTACAGCT TTAATTCAGA TAGCTCAAAT GATGGCATCA
    CATGAAAATG CTGAAGAAGT GAAGAAAGTA CAAACCCATA CCCTCCCTAT CACAATCATA
    CATGGTGTTT TTGGAGCAGG AAAGAGTTAT TTGTTGGCAG TGGTGATTTT GTTTTTTGTA
    CAGCTATTTG AAAAGAGTGA AGTTCTCACA GTTGGAAATG TAAGACCTTG GAAACTTCTC
    ATTTCTTCTT CCACTAATGT AGCTGTGGAC AGAGTACTTC TTGGGCTTCT CAGTCTTGGT
    TTTGAAAAGT TCGTCAGAGT TGGGAGTGTC AGGAAGATTG CCAAGCCAAT TTTACCTTAT
    AGTTTGCATG CTGGCTCAGA AAATGAAAAT GAACAACTAA AAGAACTACA TGGACTAATG
    AAAGAAGACT TGACTCCTCT GGAAAGGGTC TATGTCAGAA AAAGTATTGA GCAGCATAAA
    CTGGGGACCA ATAAAACCCT GCTGAAGCAG GGTTCTGATG CAGCCCATGA AAATGGATTG
    GAACAAACTC TTTTTGATCG GCTTTGCTTA ATGGGCCATA AGCCAGTTCT GTTGAGAACC
    CAGTACCGTT GTCACCCTGC AATCAGTGAT ATCTCTAATG ATCTGTTTTA TGAAGGAAAC
    CTTGTGGATG GCATTTCTGA AACAGAGAGG GGCCCTTTAT TGGAATGGCT CCCAACACTA
    TGTTTCTATA ACATTAAAGG ACCTGAACAG ATTGAAAGAG ATAACAGCTT TCATAATGTG
    GCAGAAGCTG CTTTTACACT CAAGCTGATT CAATCACTGA TTGCGAGTGG GATAGCGGGC
    TCTATGATTG GGGTGATAAC ATTATACAAA TCCCAGATGT ACAAGCTTTG TCATTTACTC
    AGTGCCGTGG ACTTTGACAA TCCTGATATT AAAGCTGTGC AGGTATCCAC AGTAGATGCT
    TTTCAGGGAG CTGAAAAGGA GATCATTATT CTGTCCTGTG TAAGGACAAG ACAAGTAGGA
    TTTATTGATT CAGAAAAAAG AATGAATGTT GCACTGACCA GAGGAAGGAG GCATTTATTG
    ATCGTGGGAA ATTTAGCCTG TTTGCGGAAA AATCGACTCT GGGGACGTGT AATCCAACAC
    TGTGAAGGAA GGAGAGATGG ATTACAACAT GCAAGCCAGT GTGAACCACA GCTGAACCAT
    CTTCTTAAAG AGTATTTTGA AAAACGAGCA GAAGAAAAGC AGAAAAAAAG TGAAAAAGAT
    AAATCTAAAG ATAAATCACA TTCACAAAAA GATATGGTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284014
    Clone ID Related Accession (Same CDS sequence) XM_022413415.1
    Accession Version XM_022413415.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6285bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein ZGRF1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGAAAGCC AGGAATTTAT TGTACTGTAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
    TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGGTTTTCA AGGACCACGT
    CAAGTGCCAA GGAAAATGGT TATTACAGAA AATGATGAAT CAGCTGCATC ACCTGAGGCT
    AAGAAACCTG GCCCTATTTT TCTTTCTCCC TTCTACAACA CACCTCCTTT GTTTTCTACT
    GCTGGCAAGA AAGATACAAA TAATATGCCA ACAGACCCTG AGAACAGTGT CATTTACAAG
    AACAGAGAGA AAAATGGCTT ATATTATTCT TCTGTTGTCT CTACTCCATC CTTTAAGAAT
    AACCCAGAAA TGCTATGTGA AGAAAATTAT TTTTGCTCAC CTGTCTATTC TGTAAATAAG
    CATTCAGATT CTTTACTATC CAATGAGGCC ATAAAAAAAG ATGGTTTGAC ATCTCACTGT
    TTAGGAGTTT CAGAAAACAT CAGAAGCAAA GCACAGATAT TAGCTCTTCT AAAGTCCAAA
    TCAACTAGTA TGGGTAAAGA ACTAGATTCT GGGATTACAG AACATTTCCC TCAGATACAA
    CTACAAGAAA GTGTAAACAT TCCTTCTAAA CCAAAGTGCA TAATTGAACA AGACAAGTGT
    GGTGAGGTGA AGAGCACAGA CAATTTATAC TACCAGCATC AATCGGAAAA TACTATGAGC
    TATAAAAGCA GATGGGCCAT GTATTTATCC TCACAGAGTT CACCTGTACG TTCTTCTACT
    GAAGATGGAA ATAATATAGA AAGGAAACCC AAAACTCAGG GAGATAATGT AAATTTAAAT
    TTGAAAGACC TTGTACAAAA AAGGATACAG TTTTTTGAAA CATGTACTGA AAAGGGTAAA
    AACTATAATG AAGACAAGCC AGTAGGTGAT AATGATCAAT CTTGCGATCA GGAAGTAAAA
    TTGGAAATTC CTTCATTCTG TAAAAGCAAC AGCTTACCAT TTACCTGTAG CAGTGTAGAG
    AATGATGGCT TATTATCAGA ATCTGACATT CAAAAAAATA ATAAAATACC TGTTAGTCAG
    GATGACCAGA TGTGTCTAAA AGGATCAATT CTCATTAGAG AAAATGCTCA GAAGATAAAT
    ATATGTGGAA CCCCAGAAAA GTATGACCAA CCCATGTCGT CCCTGCCAGA ATCTGAACAT
    CTACAAATTG AGTCTTCTAC AAGTAACAAT TCTAGGATCT CTGATGACTT TACCAATATG
    CTTTCTAAAA GTAATACTGA TAATGAAAAT CTTAATAGTA TTCCTGAACC AATGAGTAAT
    GTAACACAGC CACTTGTGGA GGTGACTTTT AATCTGAACA ATTTTGAAAC CAGTGACTCT
    GAGGAGGAAT CACAAGAAAA TAACAAAATT TCCCAGGATT CAGAGGGTTG GGTAAAGGAA
    ACTCTGGTTA ATGATGGCAG TTCAAGTGTT CAAAAGAGAT GTGAAAATAT AAGCTGTAAG
    AAAGTTGGCA GTGAACATTT GTCTCATTTA ACATCTATGG AGGATAAGCC TACAGAGACA
    TTTCCTAAAG AGACTCTGCT GTCACAGTTT TGTGATAAAG CGTGTATGGG TTTTGACACA
    GGACCTTGGA AAACTGGAAA CAGAAAAGAA ATAGAAGAGT ATAATGACAC ATTAAGCAGT
    TTTGATTCAT CTTTAGAGTG GACTGATGAT GTATATGTAG ATAATAATGA AGATGCTAAT
    AAATCTATAC AAAAAGTCAG AATTAACTAT GATATTGCTT CACTACCGAG TAAGTCTAAA
    GGTATAAGCT CAAATTTACA TATTCCTCAG TTAAACATAG CCACTAATCA GATTCCTAAA
    AACAGCCTAT TCTCGGAACA GTCTGGGCTT AAGCCTTTTA TAATGGGAAA TAAATTAGAC
    AAAAATGATG AACAGGTTTT ACTGTCAACT TCTAGCAGTG ACATCAGTAT CCAACAATTA
    AATGTTAATC AGAATCACTC TGAAGAACCT GTTGCTCTTG GTAAATCAAA TACCCAAGTT
    TCCAATTCTT TGTTGTACCT TCTCGGAGAA AAGCATCCTA TTTCCAAAGA AACAGAAGCG
    TGTATTCCTG AATCCGAAGA TTTGGGAAGG ATTAGAAGTT TAGACCATGA CCATATTGAA
    GTAGAACCTG CTGGAGAAAG CAAACAACAT TGGAATAGTC CTAGGAATCC TTCAGAACTC
    TCTGGATTAA TAAATAACAT TTCCCTTTTA AAGTCATTGT CTGAACATAG TACAGCTTTA
    GAAGGCTTGG AAATATTGAA AAAGAAAAAT ACTACCTTTA AACAACAAGG AACTCTACAG
    ACATATAATC CAGATAGTAA TTCTGAAGCT AGGAAACGAT CGACTGCAGA AGTTTTGCAA
    GCTTTACCAA AGTTGCCTCC TCTGAACCAG GATTCTCAAC AGATTATAAA AGAAAATGAA
    GTTGAACCAA GTAAACCACT TCAGTCTTTG CAGTTCTTTT CCTTTGGAGA TAAAGAGACT
    GCTTTTCTAG ATGTTATGCC TAAACAATCA GAGAGAAAAA CCTGTGACCC TAAGCCTGTT
    GAGTTTCAAG GGCACCAAGT GAAAGGATCT GCTACTAGTG CTGTAATGAT GAGAGGACAC
    AGCTCACAGC TAGAATGCGG TCAATTTCCA GATAGTATTG AGTATGAAAA CTGCACAAGA
    GACACTTGTT TATTGACACC AAAATTGACC AGTGCTTGTA TGCAGATTGA TTTCCTGCAG
    TTCCCAGATG AAAGAAACAT TCCAAAAGAA GATATTAGCT TTAATATTGA AGACAATTTT
    CAAGTGAGAG CTGAGTCAAA TAAGGACTCA ATTGAAAATG ACTTGATTTT GGATTTCTCT
    CCTAAAAATT CAGAACATTT CTATCATTAT TTGGATTCTA GTTCTAAATC AGCAGAGAAG
    ACTTCATGGG AAGCAAATAA GGTAACATCA CCAGAACAGA AGATCTCTAC ATTAAACCCT
    GATTCTACTT TTTCTTTGAA CTCACGAGAT GAAGACTTTG TGCTAGAATT CTCTGAGGAG
    TCCCTGAAAG AAAGAACTTT ACCTGTTATC AAAAAATCCA GTTCTTTGGA GAATAAGAAC
    TTTCAAAGGC TTCCATTAGT AAGTAGAACC CGAGTTCCAC TAACTACTTT GCCACCTGCT
    GATTCAGATT CTTGTTCATA CGTGATCAAC TGTGACAGGC AAGAATATTC TGGTTCCTCC
    CCCATATTCA ACTTATGTGA AGAATCAGGA GTTCTTTCAT TTAGCTTGGG GCTTGAGGAC
    CAATGTGAAA CTTCCTTCTG TAAAGAATCT AGAGAAGTGA TTCCACAGGA CGTTTTGCTT
    CTCAATAATA TACCTACTCA AAGCAAGTGG CTGAAATATC AGAACATACC CCAGTGCAAC
    TTGACTACTC CAGACAGAGT TGATAAGAAA GTAACAGATG GTTTCTCTGG TAAAGTTATT
    TCTGGGACAC ATTTAAGTGA TATACGTGAA AGACAGAGTG GTGCTGTGAA TGAAAGCTCT
    TTAGACTCTA TATATTTGCA AATGATAAAA GGCATGCTCT ATCAACAGCA GCAGGATTTT
    AGCTGTCAGG ATTTGGTTTT CAGAAAGAAA GCACTCTCAT TGAATTTAAA GCTGACTTCC
    CAGACTGAAA AAATTCAAAA CACGTTAAGA GAGTCTGCTG CCTGCTACAT CTACAGTGTA
    ACAGGAGATT TACAGGAGAT AAATGCCTCT GAGCTATGCT TCCCAAGTGG GCAGAAAATA
    AAATCTGCTT ATCTTCCCCA AAGGCAGATT CATATACCAG CTGTTTTTCA GTCTCCTGCT
    CATTATAAGC AGATTTTTAC ATCTTGTCTC ATAGAACACT TAAATATATT GTTGTTTGGG
    TTGGCACAGA GGTTGCACAA AGCTCTTTCA AAAGTTGACA TATCATTTTA CACATCATTA
    AAGGGAGAGA AACTAAAAAA TGTAGAAAGT AATGTACCAT CCTGCCATCA TCATCAACCT
    GCAAAACTTG TCATGGTTAA AAAGGAAGGT CCAAATAAGG GTCGTCTTTT TTATACCTGT
    GATGGACCCA AAGCTGACCG ATGTAAATTT TTCAAGTGGC TTGAAGAAGT GACCCCAGGA
    TATTTGACAC AAGAAGAGTC TCTACCTAGC ATAGTTTTAA GTGATATAAA GAGTATTGGC
    TTATACTTAA GAAGTCAAAA TATACCCCTC TTTGAGGAAT GTCAGCTTTT GGTGAGGAAA
    GGATTTGATT TTCAAAGAAA ACAGTATGGC AAACTGAAGA AGTTTAATGC TGTAAATCCT
    GAATTTTATA GTGAACCTAA AAGCAAACTT TATCTTAAGC TGAGTCGGAG GGAAAGTTCT
    TCAACTTACA GCAAAAATGA TCTTTGGGTG GTTTCAAAAA CATTGGACTT TGAATTGGAT
    ACTTTTATTG CGTGTAGTGC TTTCTTTGGA CCATCATCTA TCAATGAGGT GGAGCTACTA
    CCTTTGAAAG GTTATTTCCC CTCTAACTGG CCCACTAACA TAGTGGTACA TGCATTGTTG
    GTTTGTAATG CTAGCACTGA GCTGACAACT TTGAAAAACA TTCAGGACTA CTTTAATCCA
    GCTACTCTAC CTCTAACACA GTACTTGTTA ACAATGTCTT CATCAACTAT AGTTAGCAAC
    AAGAGAGTCA ATAAGAGAAA ATTTATCCCA CCAGCCTTCT CAAATATCAA TACAAAATTT
    GAACTTCTCA GCCTAGAAGC CACATTACAG TTAGCTAGTG AATTAATTCA GGCACACAAC
    TTAAATAAGG ATCAAGCTAC AGCTTTAATT CAGATAGCTC AAATGATGGC ATCACATGAA
    AATGCTGAAG AAGTGAAGAA AGTACAAACC CATACCCTCC CTATCACAAT CATACATGGT
    GTTTTTGGAG CAGGAAAGAG TTATTTGTTG GCAGTGGTGA TTTTGTTTTT TGTACAGCTA
    TTTGAAAAGA GTGAAGTTCT CACAGTTGGA AATGTAAGAC CTTGGAAACT TCTCATTTCT
    TCTTCCACTA ATGTAGCTGT GGACAGAGTA CTTCTTGGGC TTCTCAGTCT TGGTTTTGAA
    AAGTTCGTCA GAGTTGGGAG TGTCAGGAAG ATTGCCAAGC CAATTTTACC TTATAGTTTG
    CATGCTGGCT CAGAAAATGA AAATGAACAA CTAAAAGAAC TACATGGACT AATGAAAGAA
    GACTTGACTC CTCTGGAAAG GGTCTATGTC AGAAAAAGTA TTGAGCAGCA TAAACTGGGG
    ACCAATAAAA CCCTGCTGAA GCAGGTACGA GTAGTTGGAG TTACCTGTGC AGCTTGCCCA
    TTCCCATGTA TGAATGATCT TAAATTTCCT GTAGTTGTGT TGGATGAGTG TAGTCAAATA
    ACTGAACCGG CCTCTCTCCT TCCTATTGCA AGGTTTGAGT GTGAAAAGCT GATTCTTGTT
    GGAGATCCTA AACAGCTTTC TCCTACTATT CAGGGTTCTG ATGCAGCCCA TGAAAATGGA
    TTGGAACAAA CTCTTTTTGA TCGGCTTTGC TTAATGGGCC ATAAGCCAGT TCTGTTGAGA
    ACCCAGTACC GTTGTCACCC TGCAATCAGT GATATCTCTA ATGATCTGTT TTATGAAGGA
    AACCTTGTGG ATGGCATTTC TGAAACAGAG AGGGGCCCTT TATTGGAATG GCTCCCAACA
    CTATGTTTCT ATAACATTAA AGGACCTGAA CAGATTGAAA GAGATAACAG CTTTCATAAT
    GTGGCAGAAG CTGCTTTTAC ACTCAAGCTG ATTCAATCAC TGATTGCGAG TGGGATAGCG
    GGCTCTATGA TTGGGGTGAT AACATTATAC AAATCCCAGA TGTACAAGCT TTGTCATTTA
    CTCAGTGCCG TGGACTTTGA CAATCCTGAT ATTAAAGCTG TGCAGGTATC CACAGTAGAT
    GCTTTTCAGG GAGCTGAAAA GGAGATCATT ATTCTGTCCT GTGTAAGGAC AAGACAAGTA
    GGATTTATTG ATTCAGAAAA AAGAATGAAT GTTGCACTGA CCAGAGGAAG GAGGCATTTA
    TTGATCGTGG GAAATTTAGC CTGTTTGCGG AAAAATCGAC TCTGGGGACG TGTAATCCAA
    CACTGTGAAG GAAGGAGAGA TGGATTACAA CATGCAAGCC AGTGTGAACC ACAGCTGAAC
    CATCTTCTTA AAGAGTATTT TGAAAAACGA GCAGAAGAAA AGCAGAAAAA AAGTGAAAAA
    GATAAATCTA AAGATAAATC ACATTCACAA AAAGATATGG TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269123.1
    CDS316..6600
    Translation

    Target ORF information:

    RefSeq Version XM_022413415.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris zinc finger GRF-type containing 1 (ZGRF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413415.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGAAAGCC AGGAATTTAT TGTACTGTAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
    TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGGTTTTCA AGGACCACGT
    CAAGTGCCAA GGAAAATGGT TATTACAGAA AATGATGAAT CAGCTGCATC ACCTGAGGCT
    AAGAAACCTG GCCCTATTTT TCTTTCTCCC TTCTACAACA CACCTCCTTT GTTTTCTACT
    GCTGGCAAGA AAGATACAAA TAATATGCCA ACAGACCCTG AGAACAGTGT CATTTACAAG
    AACAGAGAGA AAAATGGCTT ATATTATTCT TCTGTTGTCT CTACTCCATC CTTTAAGAAT
    AACCCAGAAA TGCTATGTGA AGAAAATTAT TTTTGCTCAC CTGTCTATTC TGTAAATAAG
    CATTCAGATT CTTTACTATC CAATGAGGCC ATAAAAAAAG ATGGTTTGAC ATCTCACTGT
    TTAGGAGTTT CAGAAAACAT CAGAAGCAAA GCACAGATAT TAGCTCTTCT AAAGTCCAAA
    TCAACTAGTA TGGGTAAAGA ACTAGATTCT GGGATTACAG AACATTTCCC TCAGATACAA
    CTACAAGAAA GTGTAAACAT TCCTTCTAAA CCAAAGTGCA TAATTGAACA AGACAAGTGT
    GGTGAGGTGA AGAGCACAGA CAATTTATAC TACCAGCATC AATCGGAAAA TACTATGAGC
    TATAAAAGCA GATGGGCCAT GTATTTATCC TCACAGAGTT CACCTGTACG TTCTTCTACT
    GAAGATGGAA ATAATATAGA AAGGAAACCC AAAACTCAGG GAGATAATGT AAATTTAAAT
    TTGAAAGACC TTGTACAAAA AAGGATACAG TTTTTTGAAA CATGTACTGA AAAGGGTAAA
    AACTATAATG AAGACAAGCC AGTAGGTGAT AATGATCAAT CTTGCGATCA GGAAGTAAAA
    TTGGAAATTC CTTCATTCTG TAAAAGCAAC AGCTTACCAT TTACCTGTAG CAGTGTAGAG
    AATGATGGCT TATTATCAGA ATCTGACATT CAAAAAAATA ATAAAATACC TGTTAGTCAG
    GATGACCAGA TGTGTCTAAA AGGATCAATT CTCATTAGAG AAAATGCTCA GAAGATAAAT
    ATATGTGGAA CCCCAGAAAA GTATGACCAA CCCATGTCGT CCCTGCCAGA ATCTGAACAT
    CTACAAATTG AGTCTTCTAC AAGTAACAAT TCTAGGATCT CTGATGACTT TACCAATATG
    CTTTCTAAAA GTAATACTGA TAATGAAAAT CTTAATAGTA TTCCTGAACC AATGAGTAAT
    GTAACACAGC CACTTGTGGA GGTGACTTTT AATCTGAACA ATTTTGAAAC CAGTGACTCT
    GAGGAGGAAT CACAAGAAAA TAACAAAATT TCCCAGGATT CAGAGGGTTG GGTAAAGGAA
    ACTCTGGTTA ATGATGGCAG TTCAAGTGTT CAAAAGAGAT GTGAAAATAT AAGCTGTAAG
    AAAGTTGGCA GTGAACATTT GTCTCATTTA ACATCTATGG AGGATAAGCC TACAGAGACA
    TTTCCTAAAG AGACTCTGCT GTCACAGTTT TGTGATAAAG CGTGTATGGG TTTTGACACA
    GGACCTTGGA AAACTGGAAA CAGAAAAGAA ATAGAAGAGT ATAATGACAC ATTAAGCAGT
    TTTGATTCAT CTTTAGAGTG GACTGATGAT GTATATGTAG ATAATAATGA AGATGCTAAT
    AAATCTATAC AAAAAGTCAG AATTAACTAT GATATTGCTT CACTACCGAG TAAGTCTAAA
    GGTATAAGCT CAAATTTACA TATTCCTCAG TTAAACATAG CCACTAATCA GATTCCTAAA
    AACAGCCTAT TCTCGGAACA GTCTGGGCTT AAGCCTTTTA TAATGGGAAA TAAATTAGAC
    AAAAATGATG AACAGGTTTT ACTGTCAACT TCTAGCAGTG ACATCAGTAT CCAACAATTA
    AATGTTAATC AGAATCACTC TGAAGAACCT GTTGCTCTTG GTAAATCAAA TACCCAAGTT
    TCCAATTCTT TGTTGTACCT TCTCGGAGAA AAGCATCCTA TTTCCAAAGA AACAGAAGCG
    TGTATTCCTG AATCCGAAGA TTTGGGAAGG ATTAGAAGTT TAGACCATGA CCATATTGAA
    GTAGAACCTG CTGGAGAAAG CAAACAACAT TGGAATAGTC CTAGGAATCC TTCAGAACTC
    TCTGGATTAA TAAATAACAT TTCCCTTTTA AAGTCATTGT CTGAACATAG TACAGCTTTA
    GAAGGCTTGG AAATATTGAA AAAGAAAAAT ACTACCTTTA AACAACAAGG AACTCTACAG
    ACATATAATC CAGATAGTAA TTCTGAAGCT AGGAAACGAT CGACTGCAGA AGTTTTGCAA
    GCTTTACCAA AGTTGCCTCC TCTGAACCAG GATTCTCAAC AGATTATAAA AGAAAATGAA
    GTTGAACCAA GTAAACCACT TCAGTCTTTG CAGTTCTTTT CCTTTGGAGA TAAAGAGACT
    GCTTTTCTAG ATGTTATGCC TAAACAATCA GAGAGAAAAA CCTGTGACCC TAAGCCTGTT
    GAGTTTCAAG GGCACCAAGT GAAAGGATCT GCTACTAGTG CTGTAATGAT GAGAGGACAC
    AGCTCACAGC TAGAATGCGG TCAATTTCCA GATAGTATTG AGTATGAAAA CTGCACAAGA
    GACACTTGTT TATTGACACC AAAATTGACC AGTGCTTGTA TGCAGATTGA TTTCCTGCAG
    TTCCCAGATG AAAGAAACAT TCCAAAAGAA GATATTAGCT TTAATATTGA AGACAATTTT
    CAAGTGAGAG CTGAGTCAAA TAAGGACTCA ATTGAAAATG ACTTGATTTT GGATTTCTCT
    CCTAAAAATT CAGAACATTT CTATCATTAT TTGGATTCTA GTTCTAAATC AGCAGAGAAG
    ACTTCATGGG AAGCAAATAA GGTAACATCA CCAGAACAGA AGATCTCTAC ATTAAACCCT
    GATTCTACTT TTTCTTTGAA CTCACGAGAT GAAGACTTTG TGCTAGAATT CTCTGAGGAG
    TCCCTGAAAG AAAGAACTTT ACCTGTTATC AAAAAATCCA GTTCTTTGGA GAATAAGAAC
    TTTCAAAGGC TTCCATTAGT AAGTAGAACC CGAGTTCCAC TAACTACTTT GCCACCTGCT
    GATTCAGATT CTTGTTCATA CGTGATCAAC TGTGACAGGC AAGAATATTC TGGTTCCTCC
    CCCATATTCA ACTTATGTGA AGAATCAGGA GTTCTTTCAT TTAGCTTGGG GCTTGAGGAC
    CAATGTGAAA CTTCCTTCTG TAAAGAATCT AGAGAAGTGA TTCCACAGGA CGTTTTGCTT
    CTCAATAATA TACCTACTCA AAGCAAGTGG CTGAAATATC AGAACATACC CCAGTGCAAC
    TTGACTACTC CAGACAGAGT TGATAAGAAA GTAACAGATG GTTTCTCTGG TAAAGTTATT
    TCTGGGACAC ATTTAAGTGA TATACGTGAA AGACAGAGTG GTGCTGTGAA TGAAAGCTCT
    TTAGACTCTA TATATTTGCA AATGATAAAA GGCATGCTCT ATCAACAGCA GCAGGATTTT
    AGCTGTCAGG ATTTGGTTTT CAGAAAGAAA GCACTCTCAT TGAATTTAAA GCTGACTTCC
    CAGACTGAAA AAATTCAAAA CACGTTAAGA GAGTCTGCTG CCTGCTACAT CTACAGTGTA
    ACAGGAGATT TACAGGAGAT AAATGCCTCT GAGCTATGCT TCCCAAGTGG GCAGAAAATA
    AAATCTGCTT ATCTTCCCCA AAGGCAGATT CATATACCAG CTGTTTTTCA GTCTCCTGCT
    CATTATAAGC AGATTTTTAC ATCTTGTCTC ATAGAACACT TAAATATATT GTTGTTTGGG
    TTGGCACAGA GGTTGCACAA AGCTCTTTCA AAAGTTGACA TATCATTTTA CACATCATTA
    AAGGGAGAGA AACTAAAAAA TGTAGAAAGT AATGTACCAT CCTGCCATCA TCATCAACCT
    GCAAAACTTG TCATGGTTAA AAAGGAAGGT CCAAATAAGG GTCGTCTTTT TTATACCTGT
    GATGGACCCA AAGCTGACCG ATGTAAATTT TTCAAGTGGC TTGAAGAAGT GACCCCAGGA
    TATTTGACAC AAGAAGAGTC TCTACCTAGC ATAGTTTTAA GTGATATAAA GAGTATTGGC
    TTATACTTAA GAAGTCAAAA TATACCCCTC TTTGAGGAAT GTCAGCTTTT GGTGAGGAAA
    GGATTTGATT TTCAAAGAAA ACAGTATGGC AAACTGAAGA AGTTTAATGC TGTAAATCCT
    GAATTTTATA GTGAACCTAA AAGCAAACTT TATCTTAAGC TGAGTCGGAG GGAAAGTTCT
    TCAACTTACA GCAAAAATGA TCTTTGGGTG GTTTCAAAAA CATTGGACTT TGAATTGGAT
    ACTTTTATTG CGTGTAGTGC TTTCTTTGGA CCATCATCTA TCAATGAGGT GGAGCTACTA
    CCTTTGAAAG GTTATTTCCC CTCTAACTGG CCCACTAACA TAGTGGTACA TGCATTGTTG
    GTTTGTAATG CTAGCACTGA GCTGACAACT TTGAAAAACA TTCAGGACTA CTTTAATCCA
    GCTACTCTAC CTCTAACACA GTACTTGTTA ACAATGTCTT CATCAACTAT AGTTAGCAAC
    AAGAGAGTCA ATAAGAGAAA ATTTATCCCA CCAGCCTTCT CAAATATCAA TACAAAATTT
    GAACTTCTCA GCCTAGAAGC CACATTACAG TTAGCTAGTG AATTAATTCA GGCACACAAC
    TTAAATAAGG ATCAAGCTAC AGCTTTAATT CAGATAGCTC AAATGATGGC ATCACATGAA
    AATGCTGAAG AAGTGAAGAA AGTACAAACC CATACCCTCC CTATCACAAT CATACATGGT
    GTTTTTGGAG CAGGAAAGAG TTATTTGTTG GCAGTGGTGA TTTTGTTTTT TGTACAGCTA
    TTTGAAAAGA GTGAAGTTCT CACAGTTGGA AATGTAAGAC CTTGGAAACT TCTCATTTCT
    TCTTCCACTA ATGTAGCTGT GGACAGAGTA CTTCTTGGGC TTCTCAGTCT TGGTTTTGAA
    AAGTTCGTCA GAGTTGGGAG TGTCAGGAAG ATTGCCAAGC CAATTTTACC TTATAGTTTG
    CATGCTGGCT CAGAAAATGA AAATGAACAA CTAAAAGAAC TACATGGACT AATGAAAGAA
    GACTTGACTC CTCTGGAAAG GGTCTATGTC AGAAAAAGTA TTGAGCAGCA TAAACTGGGG
    ACCAATAAAA CCCTGCTGAA GCAGGTACGA GTAGTTGGAG TTACCTGTGC AGCTTGCCCA
    TTCCCATGTA TGAATGATCT TAAATTTCCT GTAGTTGTGT TGGATGAGTG TAGTCAAATA
    ACTGAACCGG CCTCTCTCCT TCCTATTGCA AGGTTTGAGT GTGAAAAGCT GATTCTTGTT
    GGAGATCCTA AACAGCTTTC TCCTACTATT CAGGGTTCTG ATGCAGCCCA TGAAAATGGA
    TTGGAACAAA CTCTTTTTGA TCGGCTTTGC TTAATGGGCC ATAAGCCAGT TCTGTTGAGA
    ACCCAGTACC GTTGTCACCC TGCAATCAGT GATATCTCTA ATGATCTGTT TTATGAAGGA
    AACCTTGTGG ATGGCATTTC TGAAACAGAG AGGGGCCCTT TATTGGAATG GCTCCCAACA
    CTATGTTTCT ATAACATTAA AGGACCTGAA CAGATTGAAA GAGATAACAG CTTTCATAAT
    GTGGCAGAAG CTGCTTTTAC ACTCAAGCTG ATTCAATCAC TGATTGCGAG TGGGATAGCG
    GGCTCTATGA TTGGGGTGAT AACATTATAC AAATCCCAGA TGTACAAGCT TTGTCATTTA
    CTCAGTGCCG TGGACTTTGA CAATCCTGAT ATTAAAGCTG TGCAGGTATC CACAGTAGAT
    GCTTTTCAGG GAGCTGAAAA GGAGATCATT ATTCTGTCCT GTGTAAGGAC AAGACAAGTA
    GGATTTATTG ATTCAGAAAA AAGAATGAAT GTTGCACTGA CCAGAGGAAG GAGGCATTTA
    TTGATCGTGG GAAATTTAGC CTGTTTGCGG AAAAATCGAC TCTGGGGACG TGTAATCCAA
    CACTGTGAAG GAAGGAGAGA TGGATTACAA CATGCAAGCC AGTGTGAACC ACAGCTGAAC
    CATCTTCTTA AAGAGTATTT TGAAAAACGA GCAGAAGAAA AGCAGAAAAA AAGTGAAAAA
    GATAAATCTA AAGATAAATC ACATTCACAA AAAGATATGG TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284015
    Clone ID Related Accession (Same CDS sequence) XM_022413416.1
    Accession Version XM_022413416.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6279bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein ZGRF1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGAAAGCC AGGAATTTAT TGTACTGTAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
    TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAGTTTT ATATGATGAC
    AAAGGAGAAT GTTTGGAGAG TATATTTCTT AAGTGCCTTG AGGTGAAGCC TGGAGATGAC
    TTAGAAGGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATTGCT
    ATTAAACCGG ATGTCATTAA AGAAACATCA GAGTTAAATC CAAGGAAATT TATATCCTCT
    GGGCGGTCCC TTGGATGTCA GCCTGCTGGT TTAAAAAGGA AGTTTACTGG TTTTCAAGGA
    CCACGTCAAG TGCCAAGGAA AATGGTTATT ACAGAAAATG ATGAATCAGC TGCATCACCT
    GAGGCTAAGA AACCTGGCCC TATTTTTCTT TCTCCCTTCT ACAACACACC TCCTTTGTTT
    TCTACTGCTG GCAAGAAAGA TACAAATAAT ATGCCAACAG ACCCTGAGAA CAGTGTCATT
    TACAAGAACA GAGAGAAAAA TGGCTTATAT TATTCTTCTG TTGTCTCTAC TCCATCCTTT
    AAGAATAACC CAGAAATGCT ATGTGAAGAA AATTATTTTT GCTCACCTGT CTATTCTGTA
    AATAAGCATT CAGATTCTTT ACTATCCAAT GAGGCCATAA AAAAAGATGG TTTGACATCT
    CACTGTTTAG GAGTTTCAGA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTAAAG
    TCCAAATCAA CTAGTATGGG TAAAGAACTA GATTCTGGGA TTACAGAACA TTTCCCTCAG
    ATACAACTAC AAGAAAGTGT AAACATTCCT TCTAAACCAA AGTGCATAAT TGAACAAGAC
    AAGTGTGGTG AGGTGAAGAG CACAGACAAT TTATACTACC AGCATCAATC GGAAAATACT
    ATGAGCTATA AAAGCAGATG GGCCATGTAT TTATCCTCAC AGAGTTCACC TGTACGTTCT
    TCTACTGAAG ATGGAAATAA TATAGAAAGG AAACCCAAAA CTCAGGGAGA TAATGTAAAT
    TTAAATTTGA AAGACCTTGT ACAAAAAAGG ATACAGTTTT TTGAAACATG TACTGAAAAG
    GGTAAAAACT ATAATGAAGA CAAGCCAGTA GGTGATAATG ATCAATCTTG CGATCAGGAA
    GTAAAATTGG AAATTCCTTC ATTCTGTAAA AGCAACAGCT TACCATTTAC CTGTAGCAGT
    GTAGAGAATG ATGGCTTATT ATCAGAATCT GACATTCAAA AAAATAATAA AATACCTGTT
    AGTCAGGATG ACCAGATGTG TCTAAAAGGA TCAATTCTCA TTAGAGAAAA TGCTCAGAAG
    ATAAATATAT GTGGAACCCC AGAAAAGTAT GACCAACCCA TGTCGTCCCT GCCAGAATCT
    GAACATCTAC AAATTGAGTC TTCTACAAGT AACAATTCTA GGATCTCTGA TGACTTTACC
    AATATGCTTT CTAAAAGTAA TACTGATAAT GAAAATCTTA ATAGTATTCC TGAACCAATG
    AGTAATGTAA CACAGCCACT TGTGGAGGTG ACTTTTAATC TGAACAATTT TGAAACCAGT
    GACTCTGAGG AGGAATCACA AGAAAATAAC AAAATTTCCC AGGATTCAGA GGGTTGGGTA
    AAGGAAACTC TGGTTAATGA TGGCAGTTCA AGTGTTCAAA AGAGATGTGA AAATATAAGC
    TGTAAGAAAG TTGGCAGTGA ACATTTGTCT CATTTAACAT CTATGGAGGA TAAGCCTACA
    GAGACATTTC CTAAAGAGAC TCTGCTGTCA CAGTTTTGTG ATAAAGCGTG TATGGGTTTT
    GACACAGGAC CTTGGAAAAC TGGAAACAGA AAAGAAATAG AAGAGTATAA TGACACATTA
    AGCAGTTTTG ATTCATCTTT AGAGTGGACT GATGATGTAT ATGTAGATAA TAATGAAGAT
    GCTAATAAAT CTATACAAAA AGTCAGAATT AACTATGATA TTGCTTCACT ACCGAGTAAG
    TCTAAAGGTA TAAGCTCAAA TTTACATATT CCTCAGTTAA ACATAGCCAC TAATCAGATT
    CCTAAAAACA GCCTATTCTC GGAACAGTCT GGGCTTAAGC CTTTTATAAT GGGAAATAAA
    TTAGACAAAA ATGATGAACA GGTTTTACTG TCAACTTCTA GCAGTGACAT CAGTATCCAA
    CAATTAAATG TTAATCAGAA TCACTCTGAA GAACCTGTTG CTCTTGGTAA ATCAAATACC
    CAAGTTTCCA ATTCTTTGTT GTACCTTCTC GGAGAAAAGC ATCCTATTTC CAAAGAAACA
    GAAGCGTGTA TTCCTGAATC CGAAGATTTG GGAAGGATTA GAAGTTTAGA CCATGACCAT
    ATTGAAGTAG AACCTGCTGG AGAAAGCAAA CAACATTGGA ATAGTCCTAG GAATCCTTCA
    GAACTCTCTG GATTAATAAA TAACATTTCC CTTTTAAAGT CATTGTCTGA ACATAGTACA
    GCTTTAGAAG GCTTGGAAAT ATTGAAAAAG AAAAATACTA CCTTTAAACA ACAAGGAACT
    CTACAGACAT ATAATCCAGA TAGTAATTCT GAAGCTAGGA AACGATCGAC TGCAGAAGTT
    TTGCAAGCTT TACCAAAGTT GCCTCCTCTG AACCAGGATT CTCAACAGAT TATAAAAGAA
    AATGAAGTTG AACCAAGTAA ACCACTTCAG TCTTTGCAGT TCTTTTCCTT TGGAGATAAA
    GAGACTGCTT TTCTAGATGT TATGCCTAAA CAATCAGAGA GAAAAACCTG TGACCCTAAG
    CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA GGATCTGCTA CTAGTGCTGT AATGATGAGA
    GGACACAGCT CACAGCTAGA ATGCGGTCAA TTTCCAGATA GTATTGAGTA TGAAAACTGC
    ACAAGAGACA CTTGTTTATT GACACCAAAA TTGACCAGTG CTTGTATGCA GATTGATTTC
    CTGCAGTTCC CAGATGAAAG AAACATTCCA AAAGAAGATA TTAGCTTTAA TATTGAAGAC
    AATTTTCAAG TGAGAGCTGA GTCAAATAAG GACTCAATTG AAAATGACTT GATTTTGGAT
    TTCTCTCCTA AAAATTCAGA ACATTTCTAT CATTATTTGG ATTCTAGTTC TAAATCAGCA
    GAGAAGACTT CATGGGAAGC AAATAAGGTA ACATCACCAG AACAGAAGAT CTCTACATTA
    AACCCTGATT CTACTTTTTC TTTGAACTCA CGAGATGAAG ACTTTGTGCT AGAATTCTCT
    GAGGAGTCCC TGAAAGAAAG AACTTTACCT GTTATCAAAA AATCCAGTTC TTTGGAGAAT
    AAGAACTTTC AAAGGCTTCC ATTAGTAAGT AGAACCCGAG TTCCACTAAC TACTTTGCCA
    CCTGCTGATT CAGATTCTTG TTCATACGTG ATCAACTGTG ACAGGCAAGA ATATTCTGGT
    TCCTCCCCCA TATTCAACTT ATGTGAAGAA TCAGGAGTTC TTTCATTTAG CTTGGGGCTT
    GAGGACCAAT GTGAAACTTC CTTCTGTAAA GAATCTAGAG AAGTGATTCC ACAGGACGTT
    TTGCTTCTCA ATAATATACC TACTCAAAGC AAGTGGCTGA AATATCAGAA CATACCCCAG
    TGCAACTTGA CTACTCCAGA CAGAGTTGAT AAGAAAGTAA CAGATGGTTT CTCTGGTAAA
    GTTATTTCTG GGACACATTT AAGTGATATA CGTGAAAGAC AGAGTGGTGC TGTGAATGAA
    AGCTCTTTAG ACTCTATATA TTTGCAAATG ATAAAAGGCA TGCTCTATCA ACAGCAGCAG
    GATTTTAGCT GTCAGGATTT GGTTTTCAGA AAGAAAGCAC TCTCATTGAA TTTAAAGCTG
    ACTTCCCAGA CTGAAAAAAT TCAAAACACG TTAAGAGAGT CTGCTGCCTG CTACATCTAC
    AGTGTAACAG GAGATTTACA GGAGATAAAT GCCTCTGAGC TATGCTTCCC AAGTGGGCAG
    AAAATAAAAT CTGCTTATCT TCCCCAAAGG CAGATTCATA TACCAGCTGT TTTTCAGTCT
    CCTGCTCATT ATAAGCAGAT TTTTACATCT TGTCTCATAG AACACTTAAA TATATTGTTG
    TTTGGGTTGG CACAGAGGTT GCACAAAGCT CTTTCAAAAG TTGACATATC ATTTTACACA
    TCATTAAAGG GAGAGAAACT AAAAAATGTA GAAAGTAATG TACCATCCTG CCATCATCAT
    CAACCTGCAA AACTTGTCAT GGTTAAAAAG GAAGGTCCAA ATAAGGGTCG TCTTTTTTAT
    ACCTGTGATG GACCCAAAGC TGACCGATGT AAATTTTTCA AGTGGCTTGA AGAAGTGACC
    CCAGGATATT TGACACAAGA AGAGTCTCTA CCTAGCATAG TTTTAAGTGA TATAAAGAGT
    ATTGGCTTAT ACTTAAGAAG TCAAAATATA CCCCTCTTTG AGGAATGTCA GCTTTTGGTG
    AGGAAAGGAT TTGATTTTCA AAGAAAACAG TATGGCAAAC TGAAGAAGTT TAATGCTGTA
    AATCCTGAAT TTTATAGTGA ACCTAAAAGC AAACTTTATC TTAAGCTGAG TCGGAGGGAA
    AGTTCTTCAA CTTACAGCAA AAATGATCTT TGGGTGGTTT CAAAAACATT GGACTTTGAA
    TTGGATACTT TTATTGCGTG TAGTGCTTTC TTTGGACCAT CATCTATCAA TGAGGTGGAG
    CTACTACCTT TGAAAGGTTA TTTCCCCTCT AACTGGCCCA CTAACATAGT GGTACATGCA
    TTGTTGGTTT GTAATGCTAG CACTGAGCTG ACAACTTTGA AAAACATTCA GGACTACTTT
    AATCCAGCTA CTCTACCTCT AACACAGTAC TTGTTAACAA TGTCTTCATC AACTATAGTT
    AGCAACAAGA GAGTCAATAA GAGAAAATTT ATCCCACCAG CCTTCTCAAA TATCAATACA
    AAATTTGAAC TTCTCAGCCT AGAAGCCACA TTACAGTTAG CTAGTGAATT AATTCAGGCA
    CACAACTTAA ATAAGGATCA AGCTACAGCT TTAATTCAGA TAGCTCAAAT GATGGCATCA
    CATGAAAATG CTGAAGAAGT GAAGAAAGTA CAAACCCATA CCCTCCCTAT CACAATCATA
    CATGGTGTTT TTGGAGCAGG AAAGAGTTAT TTGTTGGCAG TGGTGATTTT GTTTTTTGTA
    CAGCTATTTG AAAAGAGTGA AGTTCTCACA GTTGGAAATG TAAGACCTTG GAAACTTCTC
    ATTTCTTCTT CCACTAATGT AGCTGTGGAC AGAGTACTTC TTGGGCTTCT CAGTCTTGGT
    TTTGAAAAGT TCGTCAGAGT TGGGAGTGTC AGGAAGATTG CCAAGCCAAT TTTACCTTAT
    AGTTTGCATG CTGGCTCAGA AAATGAAAAT GAACAACTAA AAGAACTACA TGGACTAATG
    AAAGAAGACT TGACTCCTCT GGAAAGGGTC TATGTCAGAA AAAGTATTGA GCAGCATAAA
    CTGGGGACCA ATAAAACCCT GCTGAAGCAG GGCCATAAGC CAGTTCTGTT GAGAACCCAG
    TACCGTTGTC ACCCTGCAAT CAGTGATATC TCTAATGATC TGTTTTATGA AGGAAACCTT
    GTGGATGGCA TTTCTGAAAC AGAGAGGGGC CCTTTATTGG AATGGCTCCC AACACTATGT
    TTCTATAACA TTAAAGGACC TGAACAGATT GAAAGAGATA ACAGCTTTCA TAATGTGGCA
    GAAGCTGCTT TTACACTCAA GCTGATTCAA TCACTGATTG CGAGTGGGAT AGCGGGCTCT
    ATGATTGGGG TGATAACATT ATACAAATCC CAGATGTACA AGCTTTGTCA TTTACTCAGT
    GCCGTGGACT TTGACAATCC TGATATTAAA GCTGTGCAGG TATCCACAGT AGATGCTTTT
    CAGGGAGCTG AAAAGGAGAT CATTATTCTG TCCTGTGTAA GGACAAGACA AGTAGGATTT
    ATTGATTCAG AAAAAAGAAT GAATGTTGCA CTGACCAGAG GAAGGAGGCA TTTATTGATC
    GTGGGAAATT TAGCCTGTTT GCGGAAAAAT CGACTCTGGG GACGTGTAAT CCAACACTGT
    GAAGGAAGGA GAGATGGATT ACAACATGCA AGCCAGTGTG AACCACAGCT GAACCATCTT
    CTTAAAGAGT ATTTTGAAAA ACGAGCAGAA GAAAAGCAGA AAAAAAGTGA AAAAGATAAA
    TCTAAAGATA AATCACATTC ACAAAAAGAT ATGGTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269124.1
    CDS318..6596
    Translation

    Target ORF information:

    RefSeq Version XM_022413416.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris zinc finger GRF-type containing 1 (ZGRF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413416.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGAAAGCC AGGAATTTAT TGTACTGTAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
    TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAGTTTT ATATGATGAC
    AAAGGAGAAT GTTTGGAGAG TATATTTCTT AAGTGCCTTG AGGTGAAGCC TGGAGATGAC
    TTAGAAGGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATTGCT
    ATTAAACCGG ATGTCATTAA AGAAACATCA GAGTTAAATC CAAGGAAATT TATATCCTCT
    GGGCGGTCCC TTGGATGTCA GCCTGCTGGT TTAAAAAGGA AGTTTACTGG TTTTCAAGGA
    CCACGTCAAG TGCCAAGGAA AATGGTTATT ACAGAAAATG ATGAATCAGC TGCATCACCT
    GAGGCTAAGA AACCTGGCCC TATTTTTCTT TCTCCCTTCT ACAACACACC TCCTTTGTTT
    TCTACTGCTG GCAAGAAAGA TACAAATAAT ATGCCAACAG ACCCTGAGAA CAGTGTCATT
    TACAAGAACA GAGAGAAAAA TGGCTTATAT TATTCTTCTG TTGTCTCTAC TCCATCCTTT
    AAGAATAACC CAGAAATGCT ATGTGAAGAA AATTATTTTT GCTCACCTGT CTATTCTGTA
    AATAAGCATT CAGATTCTTT ACTATCCAAT GAGGCCATAA AAAAAGATGG TTTGACATCT
    CACTGTTTAG GAGTTTCAGA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTAAAG
    TCCAAATCAA CTAGTATGGG TAAAGAACTA GATTCTGGGA TTACAGAACA TTTCCCTCAG
    ATACAACTAC AAGAAAGTGT AAACATTCCT TCTAAACCAA AGTGCATAAT TGAACAAGAC
    AAGTGTGGTG AGGTGAAGAG CACAGACAAT TTATACTACC AGCATCAATC GGAAAATACT
    ATGAGCTATA AAAGCAGATG GGCCATGTAT TTATCCTCAC AGAGTTCACC TGTACGTTCT
    TCTACTGAAG ATGGAAATAA TATAGAAAGG AAACCCAAAA CTCAGGGAGA TAATGTAAAT
    TTAAATTTGA AAGACCTTGT ACAAAAAAGG ATACAGTTTT TTGAAACATG TACTGAAAAG
    GGTAAAAACT ATAATGAAGA CAAGCCAGTA GGTGATAATG ATCAATCTTG CGATCAGGAA
    GTAAAATTGG AAATTCCTTC ATTCTGTAAA AGCAACAGCT TACCATTTAC CTGTAGCAGT
    GTAGAGAATG ATGGCTTATT ATCAGAATCT GACATTCAAA AAAATAATAA AATACCTGTT
    AGTCAGGATG ACCAGATGTG TCTAAAAGGA TCAATTCTCA TTAGAGAAAA TGCTCAGAAG
    ATAAATATAT GTGGAACCCC AGAAAAGTAT GACCAACCCA TGTCGTCCCT GCCAGAATCT
    GAACATCTAC AAATTGAGTC TTCTACAAGT AACAATTCTA GGATCTCTGA TGACTTTACC
    AATATGCTTT CTAAAAGTAA TACTGATAAT GAAAATCTTA ATAGTATTCC TGAACCAATG
    AGTAATGTAA CACAGCCACT TGTGGAGGTG ACTTTTAATC TGAACAATTT TGAAACCAGT
    GACTCTGAGG AGGAATCACA AGAAAATAAC AAAATTTCCC AGGATTCAGA GGGTTGGGTA
    AAGGAAACTC TGGTTAATGA TGGCAGTTCA AGTGTTCAAA AGAGATGTGA AAATATAAGC
    TGTAAGAAAG TTGGCAGTGA ACATTTGTCT CATTTAACAT CTATGGAGGA TAAGCCTACA
    GAGACATTTC CTAAAGAGAC TCTGCTGTCA CAGTTTTGTG ATAAAGCGTG TATGGGTTTT
    GACACAGGAC CTTGGAAAAC TGGAAACAGA AAAGAAATAG AAGAGTATAA TGACACATTA
    AGCAGTTTTG ATTCATCTTT AGAGTGGACT GATGATGTAT ATGTAGATAA TAATGAAGAT
    GCTAATAAAT CTATACAAAA AGTCAGAATT AACTATGATA TTGCTTCACT ACCGAGTAAG
    TCTAAAGGTA TAAGCTCAAA TTTACATATT CCTCAGTTAA ACATAGCCAC TAATCAGATT
    CCTAAAAACA GCCTATTCTC GGAACAGTCT GGGCTTAAGC CTTTTATAAT GGGAAATAAA
    TTAGACAAAA ATGATGAACA GGTTTTACTG TCAACTTCTA GCAGTGACAT CAGTATCCAA
    CAATTAAATG TTAATCAGAA TCACTCTGAA GAACCTGTTG CTCTTGGTAA ATCAAATACC
    CAAGTTTCCA ATTCTTTGTT GTACCTTCTC GGAGAAAAGC ATCCTATTTC CAAAGAAACA
    GAAGCGTGTA TTCCTGAATC CGAAGATTTG GGAAGGATTA GAAGTTTAGA CCATGACCAT
    ATTGAAGTAG AACCTGCTGG AGAAAGCAAA CAACATTGGA ATAGTCCTAG GAATCCTTCA
    GAACTCTCTG GATTAATAAA TAACATTTCC CTTTTAAAGT CATTGTCTGA ACATAGTACA
    GCTTTAGAAG GCTTGGAAAT ATTGAAAAAG AAAAATACTA CCTTTAAACA ACAAGGAACT
    CTACAGACAT ATAATCCAGA TAGTAATTCT GAAGCTAGGA AACGATCGAC TGCAGAAGTT
    TTGCAAGCTT TACCAAAGTT GCCTCCTCTG AACCAGGATT CTCAACAGAT TATAAAAGAA
    AATGAAGTTG AACCAAGTAA ACCACTTCAG TCTTTGCAGT TCTTTTCCTT TGGAGATAAA
    GAGACTGCTT TTCTAGATGT TATGCCTAAA CAATCAGAGA GAAAAACCTG TGACCCTAAG
    CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA GGATCTGCTA CTAGTGCTGT AATGATGAGA
    GGACACAGCT CACAGCTAGA ATGCGGTCAA TTTCCAGATA GTATTGAGTA TGAAAACTGC
    ACAAGAGACA CTTGTTTATT GACACCAAAA TTGACCAGTG CTTGTATGCA GATTGATTTC
    CTGCAGTTCC CAGATGAAAG AAACATTCCA AAAGAAGATA TTAGCTTTAA TATTGAAGAC
    AATTTTCAAG TGAGAGCTGA GTCAAATAAG GACTCAATTG AAAATGACTT GATTTTGGAT
    TTCTCTCCTA AAAATTCAGA ACATTTCTAT CATTATTTGG ATTCTAGTTC TAAATCAGCA
    GAGAAGACTT CATGGGAAGC AAATAAGGTA ACATCACCAG AACAGAAGAT CTCTACATTA
    AACCCTGATT CTACTTTTTC TTTGAACTCA CGAGATGAAG ACTTTGTGCT AGAATTCTCT
    GAGGAGTCCC TGAAAGAAAG AACTTTACCT GTTATCAAAA AATCCAGTTC TTTGGAGAAT
    AAGAACTTTC AAAGGCTTCC ATTAGTAAGT AGAACCCGAG TTCCACTAAC TACTTTGCCA
    CCTGCTGATT CAGATTCTTG TTCATACGTG ATCAACTGTG ACAGGCAAGA ATATTCTGGT
    TCCTCCCCCA TATTCAACTT ATGTGAAGAA TCAGGAGTTC TTTCATTTAG CTTGGGGCTT
    GAGGACCAAT GTGAAACTTC CTTCTGTAAA GAATCTAGAG AAGTGATTCC ACAGGACGTT
    TTGCTTCTCA ATAATATACC TACTCAAAGC AAGTGGCTGA AATATCAGAA CATACCCCAG
    TGCAACTTGA CTACTCCAGA CAGAGTTGAT AAGAAAGTAA CAGATGGTTT CTCTGGTAAA
    GTTATTTCTG GGACACATTT AAGTGATATA CGTGAAAGAC AGAGTGGTGC TGTGAATGAA
    AGCTCTTTAG ACTCTATATA TTTGCAAATG ATAAAAGGCA TGCTCTATCA ACAGCAGCAG
    GATTTTAGCT GTCAGGATTT GGTTTTCAGA AAGAAAGCAC TCTCATTGAA TTTAAAGCTG
    ACTTCCCAGA CTGAAAAAAT TCAAAACACG TTAAGAGAGT CTGCTGCCTG CTACATCTAC
    AGTGTAACAG GAGATTTACA GGAGATAAAT GCCTCTGAGC TATGCTTCCC AAGTGGGCAG
    AAAATAAAAT CTGCTTATCT TCCCCAAAGG CAGATTCATA TACCAGCTGT TTTTCAGTCT
    CCTGCTCATT ATAAGCAGAT TTTTACATCT TGTCTCATAG AACACTTAAA TATATTGTTG
    TTTGGGTTGG CACAGAGGTT GCACAAAGCT CTTTCAAAAG TTGACATATC ATTTTACACA
    TCATTAAAGG GAGAGAAACT AAAAAATGTA GAAAGTAATG TACCATCCTG CCATCATCAT
    CAACCTGCAA AACTTGTCAT GGTTAAAAAG GAAGGTCCAA ATAAGGGTCG TCTTTTTTAT
    ACCTGTGATG GACCCAAAGC TGACCGATGT AAATTTTTCA AGTGGCTTGA AGAAGTGACC
    CCAGGATATT TGACACAAGA AGAGTCTCTA CCTAGCATAG TTTTAAGTGA TATAAAGAGT
    ATTGGCTTAT ACTTAAGAAG TCAAAATATA CCCCTCTTTG AGGAATGTCA GCTTTTGGTG
    AGGAAAGGAT TTGATTTTCA AAGAAAACAG TATGGCAAAC TGAAGAAGTT TAATGCTGTA
    AATCCTGAAT TTTATAGTGA ACCTAAAAGC AAACTTTATC TTAAGCTGAG TCGGAGGGAA
    AGTTCTTCAA CTTACAGCAA AAATGATCTT TGGGTGGTTT CAAAAACATT GGACTTTGAA
    TTGGATACTT TTATTGCGTG TAGTGCTTTC TTTGGACCAT CATCTATCAA TGAGGTGGAG
    CTACTACCTT TGAAAGGTTA TTTCCCCTCT AACTGGCCCA CTAACATAGT GGTACATGCA
    TTGTTGGTTT GTAATGCTAG CACTGAGCTG ACAACTTTGA AAAACATTCA GGACTACTTT
    AATCCAGCTA CTCTACCTCT AACACAGTAC TTGTTAACAA TGTCTTCATC AACTATAGTT
    AGCAACAAGA GAGTCAATAA GAGAAAATTT ATCCCACCAG CCTTCTCAAA TATCAATACA
    AAATTTGAAC TTCTCAGCCT AGAAGCCACA TTACAGTTAG CTAGTGAATT AATTCAGGCA
    CACAACTTAA ATAAGGATCA AGCTACAGCT TTAATTCAGA TAGCTCAAAT GATGGCATCA
    CATGAAAATG CTGAAGAAGT GAAGAAAGTA CAAACCCATA CCCTCCCTAT CACAATCATA
    CATGGTGTTT TTGGAGCAGG AAAGAGTTAT TTGTTGGCAG TGGTGATTTT GTTTTTTGTA
    CAGCTATTTG AAAAGAGTGA AGTTCTCACA GTTGGAAATG TAAGACCTTG GAAACTTCTC
    ATTTCTTCTT CCACTAATGT AGCTGTGGAC AGAGTACTTC TTGGGCTTCT CAGTCTTGGT
    TTTGAAAAGT TCGTCAGAGT TGGGAGTGTC AGGAAGATTG CCAAGCCAAT TTTACCTTAT
    AGTTTGCATG CTGGCTCAGA AAATGAAAAT GAACAACTAA AAGAACTACA TGGACTAATG
    AAAGAAGACT TGACTCCTCT GGAAAGGGTC TATGTCAGAA AAAGTATTGA GCAGCATAAA
    CTGGGGACCA ATAAAACCCT GCTGAAGCAG GGCCATAAGC CAGTTCTGTT GAGAACCCAG
    TACCGTTGTC ACCCTGCAAT CAGTGATATC TCTAATGATC TGTTTTATGA AGGAAACCTT
    GTGGATGGCA TTTCTGAAAC AGAGAGGGGC CCTTTATTGG AATGGCTCCC AACACTATGT
    TTCTATAACA TTAAAGGACC TGAACAGATT GAAAGAGATA ACAGCTTTCA TAATGTGGCA
    GAAGCTGCTT TTACACTCAA GCTGATTCAA TCACTGATTG CGAGTGGGAT AGCGGGCTCT
    ATGATTGGGG TGATAACATT ATACAAATCC CAGATGTACA AGCTTTGTCA TTTACTCAGT
    GCCGTGGACT TTGACAATCC TGATATTAAA GCTGTGCAGG TATCCACAGT AGATGCTTTT
    CAGGGAGCTG AAAAGGAGAT CATTATTCTG TCCTGTGTAA GGACAAGACA AGTAGGATTT
    ATTGATTCAG AAAAAAGAAT GAATGTTGCA CTGACCAGAG GAAGGAGGCA TTTATTGATC
    GTGGGAAATT TAGCCTGTTT GCGGAAAAAT CGACTCTGGG GACGTGTAAT CCAACACTGT
    GAAGGAAGGA GAGATGGATT ACAACATGCA AGCCAGTGTG AACCACAGCT GAACCATCTT
    CTTAAAGAGT ATTTTGAAAA ACGAGCAGAA GAAAAGCAGA AAAAAAGTGA AAAAGATAAA
    TCTAAAGATA AATCACATTC ACAAAAAGAT ATGGTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44624
    Clone ID Related Accession (Same CDS sequence) XM_014110061.2 , XM_014110061.1 , XM_022413411.1
    Accession Version XM_014110061.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein ZGRF1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014110061.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGAAAGCC AGGAATTTAT TGTACTGTAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
    TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAGTTTT ATATGATGAC
    AAAGGAGAAT GTTTGGAGAG TATATTTCTT AAGTGCCTTG AGGTGAAGCC TGGAGATGAC
    TTAGAAGGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATTGCT
    ATTAAACCGG ATGTCATTAA AGAAACATCA GAGTTAAATC CAAGGAAATT TATATCCTCT
    GGGCGGTCCC TTGGATGTCA GCCTGCTGGT TTAAAAAGGA AGTTTACTGG TTTTCAAGGA
    CCACGTCAAG TGCCAAGGAA AATGGTTATT ACAGAAAATG ATGAATCAGC TGCATCACCT
    GAGGCTAAGA AACCTGGCCC TATTTTTCTT TCTCCCTTCT ACAACACACC TCCTTTGTTT
    TCTACTGCTG GCAAGAAAGA TACAAATAAT ATGCCAACAG ACCCTGAGAA CAGTGTCATT
    TACAAGAACA GAGAGAAAAA TGGCTTATAT TATTCTTCTG TTGTCTCTAC TCCATCCTTT
    AAGAATAACC CAGAAATGCT ATGTGAAGAA AATTATTTTT GCTCACCTGT CTATTCTGTA
    AATAAGCATT CAGATTCTTT ACTATCCAAT GAGGCCATAA AAAAAGATGG TTTGACATCT
    CACTGTTTAG GAGTTTCAGA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTAAAG
    TCCAAATCAA CTAGTATGGG TAAAGAACTA GATTCTGGGA TTACAGAACA TTTCCCTCAG
    ATACAACTAC AAGAAAGTGT AAACATTCCT TCTAAACCAA AGTGCATAAT TGAACAAGAC
    AAGTGTGGTG AGGTGAAGAG CACAGACAAT TTATACTACC AGCATCAATC GGAAAATACT
    ATGAGCTATA AAAGCAGATG GGCCATGTAT TTATCCTCAC AGAGTTCACC TGTACGTTCT
    TCTACTGAAG ATGGAAATAA TATAGAAAGG AAACCCAAAA CTCAGGGAGA TAATGTAAAT
    TTAAATTTGA AAGACCTTGT ACAAAAAAGG ATACAGTTTT TTGAAACATG TACTGAAAAG
    GGTAAAAACT ATAATGAAGA CAAGCCAGTA GGTGATAATG ATCAATCTTG CGATCAGGAA
    GTAAAATTGG AAATTCCTTC ATTCTGTAAA AGCAACAGCT TACCATTTAC CTGTAGCAGT
    GTAGAGAATG ATGGCTTATT ATCAGAATCT GACATTCAAA AAAATAATAA AATACCTGTT
    AGTCAGGATG ACCAGATGTG TCTAAAAGGA TCAATTCTCA TTAGAGAAAA TGCTCAGAAG
    ATAAATATAT GTGGAACCCC AGAAAAGTAT GACCAACCCA TGTCGTCCCT GCCAGAATCT
    GAACATCTAC AAATTGAGTC TTCTACAAGT AACAATTCTA GGATCTCTGA TGACTTTACC
    AATATGCTTT CTAAAAGTAA TACTGATAAT GAAAATCTTA ATAGTATTCC TGAACCAATG
    AGTAATGTAA CACAGCCACT TGTGGAGGTG ACTTTTAATC TGAACAATTT TGAAACCAGT
    GACTCTGAGG AGGAATCACA AGAAAATAAC AAAATTTCCC AGGATTCAGA GGGTTGGGTA
    AAGGAAACTC TGGTTAATGA TGGCAGTTCA AGTGTTCAAA AGAGATGTGA AAATATAAGC
    TGTAAGAAAG TTGGCAGTGA ACATTTGTCT CATTTAACAT CTATGGAGGA TAAGCCTACA
    GAGACATTTC CTAAAGAGAC TCTGCTGTCA CAGTTTTGTG ATAAAGCGTG TATGGGTTTT
    GACACAGGAC CTTGGAAAAC TGGAAACAGA AAAGAAATAG AAGAGTATAA TGACACATTA
    AGCAGTTTTG ATTCATCTTT AGAGTGGACT GATGATGTAT ATGTAGATAA TAATGAAGAT
    GCTAATAAAT CTATACAAAA AGTCAGAATT AACTATGATA TTGCTTCACT ACCGAGTAAG
    TCTAAAGGTA TAAGCTCAAA TTTACATATT CCTCAGTTAA ACATAGCCAC TAATCAGATT
    CCTAAAAACA GCCTATTCTC GGAACAGTCT GGGCTTAAGC CTTTTATAAT GGGAAATAAA
    TTAGACAAAA ATGATGAACA GGTTTTACTG TCAACTTCTA GCAGTGACAT CAGTATCCAA
    CAATTAAATG TTAATCAGAA TCACTCTGAA GAACCTGTTG CTCTTGGTAA ATCAAATACC
    CAAGTTTCCA ATTCTTTGTT GTACCTTCTC GGAGAAAAGC ATCCTATTTC CAAAGAAACA
    GAAGCGTGTA TTCCTGAATC CGAAGATTTG GGAAGGATTA GAAGTTTAGA CCATGACCAT
    ATTGAAGTAG AACCTGCTGG AGAAAGCAAA CAACATTGGA ATAGTCCTAG GAATCCTTCA
    GAACTCTCTG GATTAATAAA TAACATTTCC CTTTTAAAGT CATTGTCTGA ACATAGTACA
    GCTTTAGAAG GCTTGGAAAT ATTGAAAAAG AAAAATACTA CCTTTAAACA ACAAGGAACT
    CTACAGACAT ATAATCCAGA TAGTAATTCT GAAGCTAGGA AACGATCGAC TGCAGAAGTT
    TTGCAAGCTT TACCAAAGTT GCCTCCTCTG AACCAGGATT CTCAACAGAT TATAAAAGAA
    AATGAAGTTG AACCAAGTAA ACCACTTCAG TCTTTGCAGT TCTTTTCCTT TGGAGATAAA
    GAGACTGCTT TTCTAGATGT TATGCCTAAA CAATCAGAGA GAAAAACCTG TGACCCTAAG
    CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA GGATCTGCTA CTAGTGCTGT AATGATGAGA
    GGACACAGCT CACAGCTAGA ATGCGGTCAA TTTCCAGATA GTATTGAGTA TGAAAACTGC
    ACAAGAGACA CTTGTTTATT GACACCAAAA TTGACCAGTG CTTGTATGCA GATTGATTTC
    CTGCAGTTCC CAGATGAAAG AAACATTCCA AAAGAAGATA TTAGCTTTAA TATTGAAGAC
    AATTTTCAAG TGAGAGCTGA GTCAAATAAG GACTCAATTG AAAATGACTT GATTTTGGAT
    TTCTCTCCTA AAAATTCAGA ACATTTCTAT CATTATTTGG ATTCTAGTTC TAAATCAGCA
    GAGAAGACTT CATGGGAAGC AAATAAGGTA ACATCACCAG AACAGAAGAT CTCTACATTA
    AACCCTGATT CTACTTTTTC TTTGAACTCA CGAGATGAAG ACTTTGTGCT AGAATTCTCT
    GAGGAGTCCC TGAAAGAAAG AACTTTACCT GTTATCAAAA AATCCAGTTC TTTGGAGAAT
    AAGAACTTTC AAAGGCTTCC ATTAGTAAGT AGAACCCGAG TTCCACTAAC TACTTTGCCA
    CCTGCTGATT CAGATTCTTG TTCATACGTG ATCAACTGTG ACAGGCAAGA ATATTCTGGT
    TCCTCCCCCA TATTCAACTT ATGTGAAGAA TCAGGAGTTC TTTCATTTAG CTTGGGGCTT
    GAGGACCAAT GTGAAACTTC CTTCTGTAAA GAATCTAGAG AAGTGATTCC ACAGGACGTT
    TTGCTTCTCA ATAATATACC TACTCAAAGC AAGTGGCTGA AATATCAGAA CATACCCCAG
    TGCAACTTGA CTACTCCAGA CAGAGTTGAT AAGAAAGTAA CAGATGGTTT CTCTGGTAAA
    GTTATTTCTG GGACACATTT AAGTGATATA CGTGAAAGAC AGAGTGGTGC TGTGAATGAA
    AGCTCTTTAG ACTCTATATA TTTGCAAATG ATAAAAGGCA TGCTCTATCA ACAGCAGCAG
    GATTTTAGCT GTCAGGATTT GGTTTTCAGA AAGAAAGCAC TCTCATTGAA TTTAAAGCTG
    ACTTCCCAGA CTGAAAAAAT TCAAAACACG TTAAGAGAGT CTGCTGCCTG CTACATCTAC
    AGTGTAACAG GAGATTTACA GGAGATAAAT GCCTCTGAGC TATGCTTCCC AAGTGGGCAG
    AAAATAAAAT CTGCTTATCT TCCCCAAAGG CAGATTCATA TACCAGCTGT TTTTCAGTCT
    CCTGCTCATT ATAAGCAGAT TTTTACATCT TGTCTCATAG AACACTTAAA TATATTGTTG
    TTTGGGTTGG CACAGAGGTT GCACAAAGCT CTTTCAAAAG TTGACATATC ATTTTACACA
    TCATTAAAGG GAGAGAAACT AAAAAATGTA GAAAGTAATG TACCATCCTG CCATCATCAT
    CAACCTGCAA AACTTGTCAT GGTTAAAAAG GAAGGTCCAA ATAAGGGTCG TCTTTTTTAT
    ACCTGTGATG GACCCAAAGC TGACCGATGT AAATTTTTCA AGTGGCTTGA AGAAGTGACC
    CCAGGATATT TGACACAAGA AGAGTCTCTA CCTAGCATAG TTTTAAGTGA TATAAAGAGT
    ATTGGCTTAT ACTTAAGAAG TCAAAATATA CCCCTCTTTG AGGAATGTCA GCTTTTGGTG
    AGGAAAGGAT TTGATTTTCA AAGAAAACAG TATGGCAAAC TGAAGAAGTT TAATGCTGTA
    AATCCTGAAT TTTATAGTGA ACCTAAAAGC AAACTTTATC TTAAGCTGAG TCGGAGGGAA
    AGTTCTTCAA CTTACAGCAA AAATGATCTT TGGGTGGTTT CAAAAACATT GGACTTTGAA
    TTGGATACTT TTATTGCGTG TAGTGCTTTC TTTGGACCAT CATCTATCAA TGAGGTGGAG
    CTACTACCTT TGAAAGGTTA TTTCCCCTCT AACTGGCCCA CTAACATAGT GGTACATGCA
    TTGTTGGTTT GTAATGCTAG CACTGAGCTG ACAACTTTGA AAAACATTCA GGACTACTTT
    AATCCAGCTA CTCTACCTCT AACACAGTAC TTGTTAACAA TGTCTTCATC AACTATAGTT
    AGCAACAAGA GAGTCAATAA GAGAAAATTT ATCCCACCAG CCTTCTCAAA TATCAATACA
    AAATTTGAAC TTCTCAGCCT AGAAGCCACA TTACAGTTAG CTAGTGAATT AATTCAGGCA
    CACAACTTAA ATAAGGATCA AGCTACAGCT TTAATTCAGA TAGCTCAAAT GATGGCATCA
    CATGAAAATG CTGAAGAAGT GAAGAAAGTA CAAACCCATA CCCTCCCTAT CACAATCATA
    CATGGTGTTT TTGGAGCAGG AAAGAGTTAT TTGTTGGCAG TGGTGATTTT GTTTTTTGTA
    CAGCTATTTG AAAAGAGTGA AGTTCTCACA GTTGGAAATG TAAGACCTTG GAAACTTCTC
    ATTTCTTCTT CCACTAATGT AGCTGTGGAC AGAGTACTTC TTGGGCTTCT CAGTCTTGGT
    TTTGAAAAGT TCGTCAGAGT TGGGAGTGTC AGGAAGATTG CCAAGCCAAT TTTACCTTAT
    AGTTTGCATG CTGGCTCAGA AAATGAAAAT GAACAACTAA AAGAACTACA TGGACTAATG
    AAAGAAGACT TGACTCCTCT GGAAAGGGTC TATGTCAGAA AAAGTATTGA GCAGCATAAA
    CTGGGGACCA ATAAAACCCT GCTGAAGCAG GTACGAGTAG TTGGAGTTAC CTGTGCAGCT
    TGCCCATTCC CATGTATGAA TGATCTTAAA TTTCCTGTAG TTGTGTTGGA TGAGTGTAGT
    CAAATAACTG AACCGGCCTC TCTCCTTCCT ATTGCAAGGT TTGAGTGTGA AAAGCTGATT
    CTTGTTGGAG ATCCTAAACA GCTTTCTCCT ACTATTCAGG GTTCTGATGC AGCCCATGAA
    AATGGATTGG AACAAACTCT TTTTGATCGG CTTTGCTTAA TGGGCCATAA GCCAGTTCTG
    TTGAGAACCC AGTACCGTTG TCACCCTGCA ATCAGTGATA TCTCTAATGA TCTGTTTTAT
    GAAGGAAACC TTGTGGATGG CATTTCTGAA ACAGAGAGGG GCCCTTTATT GGAATGGCTC
    CCAACACTAT GTTTCTATAA CATTAAAGGA CCTGAACAGA TTGAAAGAGA TAACAGCTTT
    CATAATGTGG CAGAAGCTGC TTTTACACTC AAGCTGATTC AATCACTGAT TGCGAGTGGG
    ATAGCGGGCT CTATGATTGG GGTGATAACA TTATACAAAT CCCAGATGTA CAAGCTTTGT
    CATTTACTCA GTGCCGTGGA CTTTGACAAT CCTGATATTA AAGCTGTGCA GGTATCCACA
    GTAGATGCTT TTCAGGGAGC TGAAAAGGAG ATCATTATTC TGTCCTGTGT AAGGACAAGA
    CAAGTAGGAT TTATTGATTC AGAAAAAAGA ATGAATGTTG CACTGACCAG AGGAAGGAGG
    CATTTATTGA TCGTGGGAAA TTTAGCCTGT TTGCGGAAAA ATCGACTCTG GGGACGTGTA
    ATCCAACACT GTGAAGGAAG GAGAGATGGA TTACAACATG CAAGCCAGTG TGAACCACAG
    CTGAACCATC TTCTTAAAGA GTATTTTGAA AAACGAGCAG AAGAAAAGCA GAAAAAAAGT
    GAAAAAGATA AATCTAAAGA TAAATCACAT TCACAAAAAG ATATGGTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965536.1
    CDS316..6846
    Translation

    Target ORF information:

    RefSeq Version XM_014110061.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris zinc finger GRF-type containing 1 (ZGRF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110061.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGAAAGCC AGGAATTTAT TGTACTGTAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
    TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAGTTTT ATATGATGAC
    AAAGGAGAAT GTTTGGAGAG TATATTTCTT AAGTGCCTTG AGGTGAAGCC TGGAGATGAC
    TTAGAAGGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATTGCT
    ATTAAACCGG ATGTCATTAA AGAAACATCA GAGTTAAATC CAAGGAAATT TATATCCTCT
    GGGCGGTCCC TTGGATGTCA GCCTGCTGGT TTAAAAAGGA AGTTTACTGG TTTTCAAGGA
    CCACGTCAAG TGCCAAGGAA AATGGTTATT ACAGAAAATG ATGAATCAGC TGCATCACCT
    GAGGCTAAGA AACCTGGCCC TATTTTTCTT TCTCCCTTCT ACAACACACC TCCTTTGTTT
    TCTACTGCTG GCAAGAAAGA TACAAATAAT ATGCCAACAG ACCCTGAGAA CAGTGTCATT
    TACAAGAACA GAGAGAAAAA TGGCTTATAT TATTCTTCTG TTGTCTCTAC TCCATCCTTT
    AAGAATAACC CAGAAATGCT ATGTGAAGAA AATTATTTTT GCTCACCTGT CTATTCTGTA
    AATAAGCATT CAGATTCTTT ACTATCCAAT GAGGCCATAA AAAAAGATGG TTTGACATCT
    CACTGTTTAG GAGTTTCAGA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTAAAG
    TCCAAATCAA CTAGTATGGG TAAAGAACTA GATTCTGGGA TTACAGAACA TTTCCCTCAG
    ATACAACTAC AAGAAAGTGT AAACATTCCT TCTAAACCAA AGTGCATAAT TGAACAAGAC
    AAGTGTGGTG AGGTGAAGAG CACAGACAAT TTATACTACC AGCATCAATC GGAAAATACT
    ATGAGCTATA AAAGCAGATG GGCCATGTAT TTATCCTCAC AGAGTTCACC TGTACGTTCT
    TCTACTGAAG ATGGAAATAA TATAGAAAGG AAACCCAAAA CTCAGGGAGA TAATGTAAAT
    TTAAATTTGA AAGACCTTGT ACAAAAAAGG ATACAGTTTT TTGAAACATG TACTGAAAAG
    GGTAAAAACT ATAATGAAGA CAAGCCAGTA GGTGATAATG ATCAATCTTG CGATCAGGAA
    GTAAAATTGG AAATTCCTTC ATTCTGTAAA AGCAACAGCT TACCATTTAC CTGTAGCAGT
    GTAGAGAATG ATGGCTTATT ATCAGAATCT GACATTCAAA AAAATAATAA AATACCTGTT
    AGTCAGGATG ACCAGATGTG TCTAAAAGGA TCAATTCTCA TTAGAGAAAA TGCTCAGAAG
    ATAAATATAT GTGGAACCCC AGAAAAGTAT GACCAACCCA TGTCGTCCCT GCCAGAATCT
    GAACATCTAC AAATTGAGTC TTCTACAAGT AACAATTCTA GGATCTCTGA TGACTTTACC
    AATATGCTTT CTAAAAGTAA TACTGATAAT GAAAATCTTA ATAGTATTCC TGAACCAATG
    AGTAATGTAA CACAGCCACT TGTGGAGGTG ACTTTTAATC TGAACAATTT TGAAACCAGT
    GACTCTGAGG AGGAATCACA AGAAAATAAC AAAATTTCCC AGGATTCAGA GGGTTGGGTA
    AAGGAAACTC TGGTTAATGA TGGCAGTTCA AGTGTTCAAA AGAGATGTGA AAATATAAGC
    TGTAAGAAAG TTGGCAGTGA ACATTTGTCT CATTTAACAT CTATGGAGGA TAAGCCTACA
    GAGACATTTC CTAAAGAGAC TCTGCTGTCA CAGTTTTGTG ATAAAGCGTG TATGGGTTTT
    GACACAGGAC CTTGGAAAAC TGGAAACAGA AAAGAAATAG AAGAGTATAA TGACACATTA
    AGCAGTTTTG ATTCATCTTT AGAGTGGACT GATGATGTAT ATGTAGATAA TAATGAAGAT
    GCTAATAAAT CTATACAAAA AGTCAGAATT AACTATGATA TTGCTTCACT ACCGAGTAAG
    TCTAAAGGTA TAAGCTCAAA TTTACATATT CCTCAGTTAA ACATAGCCAC TAATCAGATT
    CCTAAAAACA GCCTATTCTC GGAACAGTCT GGGCTTAAGC CTTTTATAAT GGGAAATAAA
    TTAGACAAAA ATGATGAACA GGTTTTACTG TCAACTTCTA GCAGTGACAT CAGTATCCAA
    CAATTAAATG TTAATCAGAA TCACTCTGAA GAACCTGTTG CTCTTGGTAA ATCAAATACC
    CAAGTTTCCA ATTCTTTGTT GTACCTTCTC GGAGAAAAGC ATCCTATTTC CAAAGAAACA
    GAAGCGTGTA TTCCTGAATC CGAAGATTTG GGAAGGATTA GAAGTTTAGA CCATGACCAT
    ATTGAAGTAG AACCTGCTGG AGAAAGCAAA CAACATTGGA ATAGTCCTAG GAATCCTTCA
    GAACTCTCTG GATTAATAAA TAACATTTCC CTTTTAAAGT CATTGTCTGA ACATAGTACA
    GCTTTAGAAG GCTTGGAAAT ATTGAAAAAG AAAAATACTA CCTTTAAACA ACAAGGAACT
    CTACAGACAT ATAATCCAGA TAGTAATTCT GAAGCTAGGA AACGATCGAC TGCAGAAGTT
    TTGCAAGCTT TACCAAAGTT GCCTCCTCTG AACCAGGATT CTCAACAGAT TATAAAAGAA
    AATGAAGTTG AACCAAGTAA ACCACTTCAG TCTTTGCAGT TCTTTTCCTT TGGAGATAAA
    GAGACTGCTT TTCTAGATGT TATGCCTAAA CAATCAGAGA GAAAAACCTG TGACCCTAAG
    CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA GGATCTGCTA CTAGTGCTGT AATGATGAGA
    GGACACAGCT CACAGCTAGA ATGCGGTCAA TTTCCAGATA GTATTGAGTA TGAAAACTGC
    ACAAGAGACA CTTGTTTATT GACACCAAAA TTGACCAGTG CTTGTATGCA GATTGATTTC
    CTGCAGTTCC CAGATGAAAG AAACATTCCA AAAGAAGATA TTAGCTTTAA TATTGAAGAC
    AATTTTCAAG TGAGAGCTGA GTCAAATAAG GACTCAATTG AAAATGACTT GATTTTGGAT
    TTCTCTCCTA AAAATTCAGA ACATTTCTAT CATTATTTGG ATTCTAGTTC TAAATCAGCA
    GAGAAGACTT CATGGGAAGC AAATAAGGTA ACATCACCAG AACAGAAGAT CTCTACATTA
    AACCCTGATT CTACTTTTTC TTTGAACTCA CGAGATGAAG ACTTTGTGCT AGAATTCTCT
    GAGGAGTCCC TGAAAGAAAG AACTTTACCT GTTATCAAAA AATCCAGTTC TTTGGAGAAT
    AAGAACTTTC AAAGGCTTCC ATTAGTAAGT AGAACCCGAG TTCCACTAAC TACTTTGCCA
    CCTGCTGATT CAGATTCTTG TTCATACGTG ATCAACTGTG ACAGGCAAGA ATATTCTGGT
    TCCTCCCCCA TATTCAACTT ATGTGAAGAA TCAGGAGTTC TTTCATTTAG CTTGGGGCTT
    GAGGACCAAT GTGAAACTTC CTTCTGTAAA GAATCTAGAG AAGTGATTCC ACAGGACGTT
    TTGCTTCTCA ATAATATACC TACTCAAAGC AAGTGGCTGA AATATCAGAA CATACCCCAG
    TGCAACTTGA CTACTCCAGA CAGAGTTGAT AAGAAAGTAA CAGATGGTTT CTCTGGTAAA
    GTTATTTCTG GGACACATTT AAGTGATATA CGTGAAAGAC AGAGTGGTGC TGTGAATGAA
    AGCTCTTTAG ACTCTATATA TTTGCAAATG ATAAAAGGCA TGCTCTATCA ACAGCAGCAG
    GATTTTAGCT GTCAGGATTT GGTTTTCAGA AAGAAAGCAC TCTCATTGAA TTTAAAGCTG
    ACTTCCCAGA CTGAAAAAAT TCAAAACACG TTAAGAGAGT CTGCTGCCTG CTACATCTAC
    AGTGTAACAG GAGATTTACA GGAGATAAAT GCCTCTGAGC TATGCTTCCC AAGTGGGCAG
    AAAATAAAAT CTGCTTATCT TCCCCAAAGG CAGATTCATA TACCAGCTGT TTTTCAGTCT
    CCTGCTCATT ATAAGCAGAT TTTTACATCT TGTCTCATAG AACACTTAAA TATATTGTTG
    TTTGGGTTGG CACAGAGGTT GCACAAAGCT CTTTCAAAAG TTGACATATC ATTTTACACA
    TCATTAAAGG GAGAGAAACT AAAAAATGTA GAAAGTAATG TACCATCCTG CCATCATCAT
    CAACCTGCAA AACTTGTCAT GGTTAAAAAG GAAGGTCCAA ATAAGGGTCG TCTTTTTTAT
    ACCTGTGATG GACCCAAAGC TGACCGATGT AAATTTTTCA AGTGGCTTGA AGAAGTGACC
    CCAGGATATT TGACACAAGA AGAGTCTCTA CCTAGCATAG TTTTAAGTGA TATAAAGAGT
    ATTGGCTTAT ACTTAAGAAG TCAAAATATA CCCCTCTTTG AGGAATGTCA GCTTTTGGTG
    AGGAAAGGAT TTGATTTTCA AAGAAAACAG TATGGCAAAC TGAAGAAGTT TAATGCTGTA
    AATCCTGAAT TTTATAGTGA ACCTAAAAGC AAACTTTATC TTAAGCTGAG TCGGAGGGAA
    AGTTCTTCAA CTTACAGCAA AAATGATCTT TGGGTGGTTT CAAAAACATT GGACTTTGAA
    TTGGATACTT TTATTGCGTG TAGTGCTTTC TTTGGACCAT CATCTATCAA TGAGGTGGAG
    CTACTACCTT TGAAAGGTTA TTTCCCCTCT AACTGGCCCA CTAACATAGT GGTACATGCA
    TTGTTGGTTT GTAATGCTAG CACTGAGCTG ACAACTTTGA AAAACATTCA GGACTACTTT
    AATCCAGCTA CTCTACCTCT AACACAGTAC TTGTTAACAA TGTCTTCATC AACTATAGTT
    AGCAACAAGA GAGTCAATAA GAGAAAATTT ATCCCACCAG CCTTCTCAAA TATCAATACA
    AAATTTGAAC TTCTCAGCCT AGAAGCCACA TTACAGTTAG CTAGTGAATT AATTCAGGCA
    CACAACTTAA ATAAGGATCA AGCTACAGCT TTAATTCAGA TAGCTCAAAT GATGGCATCA
    CATGAAAATG CTGAAGAAGT GAAGAAAGTA CAAACCCATA CCCTCCCTAT CACAATCATA
    CATGGTGTTT TTGGAGCAGG AAAGAGTTAT TTGTTGGCAG TGGTGATTTT GTTTTTTGTA
    CAGCTATTTG AAAAGAGTGA AGTTCTCACA GTTGGAAATG TAAGACCTTG GAAACTTCTC
    ATTTCTTCTT CCACTAATGT AGCTGTGGAC AGAGTACTTC TTGGGCTTCT CAGTCTTGGT
    TTTGAAAAGT TCGTCAGAGT TGGGAGTGTC AGGAAGATTG CCAAGCCAAT TTTACCTTAT
    AGTTTGCATG CTGGCTCAGA AAATGAAAAT GAACAACTAA AAGAACTACA TGGACTAATG
    AAAGAAGACT TGACTCCTCT GGAAAGGGTC TATGTCAGAA AAAGTATTGA GCAGCATAAA
    CTGGGGACCA ATAAAACCCT GCTGAAGCAG GTACGAGTAG TTGGAGTTAC CTGTGCAGCT
    TGCCCATTCC CATGTATGAA TGATCTTAAA TTTCCTGTAG TTGTGTTGGA TGAGTGTAGT
    CAAATAACTG AACCGGCCTC TCTCCTTCCT ATTGCAAGGT TTGAGTGTGA AAAGCTGATT
    CTTGTTGGAG ATCCTAAACA GCTTTCTCCT ACTATTCAGG GTTCTGATGC AGCCCATGAA
    AATGGATTGG AACAAACTCT TTTTGATCGG CTTTGCTTAA TGGGCCATAA GCCAGTTCTG
    TTGAGAACCC AGTACCGTTG TCACCCTGCA ATCAGTGATA TCTCTAATGA TCTGTTTTAT
    GAAGGAAACC TTGTGGATGG CATTTCTGAA ACAGAGAGGG GCCCTTTATT GGAATGGCTC
    CCAACACTAT GTTTCTATAA CATTAAAGGA CCTGAACAGA TTGAAAGAGA TAACAGCTTT
    CATAATGTGG CAGAAGCTGC TTTTACACTC AAGCTGATTC AATCACTGAT TGCGAGTGGG
    ATAGCGGGCT CTATGATTGG GGTGATAACA TTATACAAAT CCCAGATGTA CAAGCTTTGT
    CATTTACTCA GTGCCGTGGA CTTTGACAAT CCTGATATTA AAGCTGTGCA GGTATCCACA
    GTAGATGCTT TTCAGGGAGC TGAAAAGGAG ATCATTATTC TGTCCTGTGT AAGGACAAGA
    CAAGTAGGAT TTATTGATTC AGAAAAAAGA ATGAATGTTG CACTGACCAG AGGAAGGAGG
    CATTTATTGA TCGTGGGAAA TTTAGCCTGT TTGCGGAAAA ATCGACTCTG GGGACGTGTA
    ATCCAACACT GTGAAGGAAG GAGAGATGGA TTACAACATG CAAGCCAGTG TGAACCACAG
    CTGAACCATC TTCTTAAAGA GTATTTTGAA AAACGAGCAG AAGAAAAGCA GAAAAAAAGT
    GAAAAAGATA AATCTAAAGA TAAATCACAT TCACAAAAAG ATATGGTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44624
    Clone ID Related Accession (Same CDS sequence) XM_014110061.2 , XM_014110061.1 , XM_022413411.1
    Accession Version XM_014110061.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein ZGRF1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726116.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGAAAGCC AGGAATTTAT TGTACTGTAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
    TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAGTTTT ATATGATGAC
    AAAGGAGAAT GTTTGGAGAG TATATTTCTT AAGTGCCTTG AGGTGAAGCC TGGAGATGAC
    TTAGAAGGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATTGCT
    ATTAAACCGG ATGTCATTAA AGAAACATCA GAGTTAAATC CAAGGAAATT TATATCCTCT
    GGGCGGTCCC TTGGATGTCA GCCTGCTGGT TTAAAAAGGA AGTTTACTGG TTTTCAAGGA
    CCACGTCAAG TGCCAAGGAA AATGGTTATT ACAGAAAATG ATGAATCAGC TGCATCACCT
    GAGGCTAAGA AACCTGGCCC TATTTTTCTT TCTCCCTTCT ACAACACACC TCCTTTGTTT
    TCTACTGCTG GCAAGAAAGA TACAAATAAT ATGCCAACAG ACCCTGAGAA CAGTGTCATT
    TACAAGAACA GAGAGAAAAA TGGCTTATAT TATTCTTCTG TTGTCTCTAC TCCATCCTTT
    AAGAATAACC CAGAAATGCT ATGTGAAGAA AATTATTTTT GCTCACCTGT CTATTCTGTA
    AATAAGCATT CAGATTCTTT ACTATCCAAT GAGGCCATAA AAAAAGATGG TTTGACATCT
    CACTGTTTAG GAGTTTCAGA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTAAAG
    TCCAAATCAA CTAGTATGGG TAAAGAACTA GATTCTGGGA TTACAGAACA TTTCCCTCAG
    ATACAACTAC AAGAAAGTGT AAACATTCCT TCTAAACCAA AGTGCATAAT TGAACAAGAC
    AAGTGTGGTG AGGTGAAGAG CACAGACAAT TTATACTACC AGCATCAATC GGAAAATACT
    ATGAGCTATA AAAGCAGATG GGCCATGTAT TTATCCTCAC AGAGTTCACC TGTACGTTCT
    TCTACTGAAG ATGGAAATAA TATAGAAAGG AAACCCAAAA CTCAGGGAGA TAATGTAAAT
    TTAAATTTGA AAGACCTTGT ACAAAAAAGG ATACAGTTTT TTGAAACATG TACTGAAAAG
    GGTAAAAACT ATAATGAAGA CAAGCCAGTA GGTGATAATG ATCAATCTTG CGATCAGGAA
    GTAAAATTGG AAATTCCTTC ATTCTGTAAA AGCAACAGCT TACCATTTAC CTGTAGCAGT
    GTAGAGAATG ATGGCTTATT ATCAGAATCT GACATTCAAA AAAATAATAA AATACCTGTT
    AGTCAGGATG ACCAGATGTG TCTAAAAGGA TCAATTCTCA TTAGAGAAAA TGCTCAGAAG
    ATAAATATAT GTGGAACCCC AGAAAAGTAT GACCAACCCA TGTCGTCCCT GCCAGAATCT
    GAACATCTAC AAATTGAGTC TTCTACAAGT AACAATTCTA GGATCTCTGA TGACTTTACC
    AATATGCTTT CTAAAAGTAA TACTGATAAT GAAAATCTTA ATAGTATTCC TGAACCAATG
    AGTAATGTAA CACAGCCACT TGTGGAGGTG ACTTTTAATC TGAACAATTT TGAAACCAGT
    GACTCTGAGG AGGAATCACA AGAAAATAAC AAAATTTCCC AGGATTCAGA GGGTTGGGTA
    AAGGAAACTC TGGTTAATGA TGGCAGTTCA AGTGTTCAAA AGAGATGTGA AAATATAAGC
    TGTAAGAAAG TTGGCAGTGA ACATTTGTCT CATTTAACAT CTATGGAGGA TAAGCCTACA
    GAGACATTTC CTAAAGAGAC TCTGCTGTCA CAGTTTTGTG ATAAAGCGTG TATGGGTTTT
    GACACAGGAC CTTGGAAAAC TGGAAACAGA AAAGAAATAG AAGAGTATAA TGACACATTA
    AGCAGTTTTG ATTCATCTTT AGAGTGGACT GATGATGTAT ATGTAGATAA TAATGAAGAT
    GCTAATAAAT CTATACAAAA AGTCAGAATT AACTATGATA TTGCTTCACT ACCGAGTAAG
    TCTAAAGGTA TAAGCTCAAA TTTACATATT CCTCAGTTAA ACATAGCCAC TAATCAGATT
    CCTAAAAACA GCCTATTCTC GGAACAGTCT GGGCTTAAGC CTTTTATAAT GGGAAATAAA
    TTAGACAAAA ATGATGAACA GGTTTTACTG TCAACTTCTA GCAGTGACAT CAGTATCCAA
    CAATTAAATG TTAATCAGAA TCACTCTGAA GAACCTGTTG CTCTTGGTAA ATCAAATACC
    CAAGTTTCCA ATTCTTTGTT GTACCTTCTC GGAGAAAAGC ATCCTATTTC CAAAGAAACA
    GAAGCGTGTA TTCCTGAATC CGAAGATTTG GGAAGGATTA GAAGTTTAGA CCATGACCAT
    ATTGAAGTAG AACCTGCTGG AGAAAGCAAA CAACATTGGA ATAGTCCTAG GAATCCTTCA
    GAACTCTCTG GATTAATAAA TAACATTTCC CTTTTAAAGT CATTGTCTGA ACATAGTACA
    GCTTTAGAAG GCTTGGAAAT ATTGAAAAAG AAAAATACTA CCTTTAAACA ACAAGGAACT
    CTACAGACAT ATAATCCAGA TAGTAATTCT GAAGCTAGGA AACGATCGAC TGCAGAAGTT
    TTGCAAGCTT TACCAAAGTT GCCTCCTCTG AACCAGGATT CTCAACAGAT TATAAAAGAA
    AATGAAGTTG AACCAAGTAA ACCACTTCAG TCTTTGCAGT TCTTTTCCTT TGGAGATAAA
    GAGACTGCTT TTCTAGATGT TATGCCTAAA CAATCAGAGA GAAAAACCTG TGACCCTAAG
    CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA GGATCTGCTA CTAGTGCTGT AATGATGAGA
    GGACACAGCT CACAGCTAGA ATGCGGTCAA TTTCCAGATA GTATTGAGTA TGAAAACTGC
    ACAAGAGACA CTTGTTTATT GACACCAAAA TTGACCAGTG CTTGTATGCA GATTGATTTC
    CTGCAGTTCC CAGATGAAAG AAACATTCCA AAAGAAGATA TTAGCTTTAA TATTGAAGAC
    AATTTTCAAG TGAGAGCTGA GTCAAATAAG GACTCAATTG AAAATGACTT GATTTTGGAT
    TTCTCTCCTA AAAATTCAGA ACATTTCTAT CATTATTTGG ATTCTAGTTC TAAATCAGCA
    GAGAAGACTT CATGGGAAGC AAATAAGGTA ACATCACCAG AACAGAAGAT CTCTACATTA
    AACCCTGATT CTACTTTTTC TTTGAACTCA CGAGATGAAG ACTTTGTGCT AGAATTCTCT
    GAGGAGTCCC TGAAAGAAAG AACTTTACCT GTTATCAAAA AATCCAGTTC TTTGGAGAAT
    AAGAACTTTC AAAGGCTTCC ATTAGTAAGT AGAACCCGAG TTCCACTAAC TACTTTGCCA
    CCTGCTGATT CAGATTCTTG TTCATACGTG ATCAACTGTG ACAGGCAAGA ATATTCTGGT
    TCCTCCCCCA TATTCAACTT ATGTGAAGAA TCAGGAGTTC TTTCATTTAG CTTGGGGCTT
    GAGGACCAAT GTGAAACTTC CTTCTGTAAA GAATCTAGAG AAGTGATTCC ACAGGACGTT
    TTGCTTCTCA ATAATATACC TACTCAAAGC AAGTGGCTGA AATATCAGAA CATACCCCAG
    TGCAACTTGA CTACTCCAGA CAGAGTTGAT AAGAAAGTAA CAGATGGTTT CTCTGGTAAA
    GTTATTTCTG GGACACATTT AAGTGATATA CGTGAAAGAC AGAGTGGTGC TGTGAATGAA
    AGCTCTTTAG ACTCTATATA TTTGCAAATG ATAAAAGGCA TGCTCTATCA ACAGCAGCAG
    GATTTTAGCT GTCAGGATTT GGTTTTCAGA AAGAAAGCAC TCTCATTGAA TTTAAAGCTG
    ACTTCCCAGA CTGAAAAAAT TCAAAACACG TTAAGAGAGT CTGCTGCCTG CTACATCTAC
    AGTGTAACAG GAGATTTACA GGAGATAAAT GCCTCTGAGC TATGCTTCCC AAGTGGGCAG
    AAAATAAAAT CTGCTTATCT TCCCCAAAGG CAGATTCATA TACCAGCTGT TTTTCAGTCT
    CCTGCTCATT ATAAGCAGAT TTTTACATCT TGTCTCATAG AACACTTAAA TATATTGTTG
    TTTGGGTTGG CACAGAGGTT GCACAAAGCT CTTTCAAAAG TTGACATATC ATTTTACACA
    TCATTAAAGG GAGAGAAACT AAAAAATGTA GAAAGTAATG TACCATCCTG CCATCATCAT
    CAACCTGCAA AACTTGTCAT GGTTAAAAAG GAAGGTCCAA ATAAGGGTCG TCTTTTTTAT
    ACCTGTGATG GACCCAAAGC TGACCGATGT AAATTTTTCA AGTGGCTTGA AGAAGTGACC
    CCAGGATATT TGACACAAGA AGAGTCTCTA CCTAGCATAG TTTTAAGTGA TATAAAGAGT
    ATTGGCTTAT ACTTAAGAAG TCAAAATATA CCCCTCTTTG AGGAATGTCA GCTTTTGGTG
    AGGAAAGGAT TTGATTTTCA AAGAAAACAG TATGGCAAAC TGAAGAAGTT TAATGCTGTA
    AATCCTGAAT TTTATAGTGA ACCTAAAAGC AAACTTTATC TTAAGCTGAG TCGGAGGGAA
    AGTTCTTCAA CTTACAGCAA AAATGATCTT TGGGTGGTTT CAAAAACATT GGACTTTGAA
    TTGGATACTT TTATTGCGTG TAGTGCTTTC TTTGGACCAT CATCTATCAA TGAGGTGGAG
    CTACTACCTT TGAAAGGTTA TTTCCCCTCT AACTGGCCCA CTAACATAGT GGTACATGCA
    TTGTTGGTTT GTAATGCTAG CACTGAGCTG ACAACTTTGA AAAACATTCA GGACTACTTT
    AATCCAGCTA CTCTACCTCT AACACAGTAC TTGTTAACAA TGTCTTCATC AACTATAGTT
    AGCAACAAGA GAGTCAATAA GAGAAAATTT ATCCCACCAG CCTTCTCAAA TATCAATACA
    AAATTTGAAC TTCTCAGCCT AGAAGCCACA TTACAGTTAG CTAGTGAATT AATTCAGGCA
    CACAACTTAA ATAAGGATCA AGCTACAGCT TTAATTCAGA TAGCTCAAAT GATGGCATCA
    CATGAAAATG CTGAAGAAGT GAAGAAAGTA CAAACCCATA CCCTCCCTAT CACAATCATA
    CATGGTGTTT TTGGAGCAGG AAAGAGTTAT TTGTTGGCAG TGGTGATTTT GTTTTTTGTA
    CAGCTATTTG AAAAGAGTGA AGTTCTCACA GTTGGAAATG TAAGACCTTG GAAACTTCTC
    ATTTCTTCTT CCACTAATGT AGCTGTGGAC AGAGTACTTC TTGGGCTTCT CAGTCTTGGT
    TTTGAAAAGT TCGTCAGAGT TGGGAGTGTC AGGAAGATTG CCAAGCCAAT TTTACCTTAT
    AGTTTGCATG CTGGCTCAGA AAATGAAAAT GAACAACTAA AAGAACTACA TGGACTAATG
    AAAGAAGACT TGACTCCTCT GGAAAGGGTC TATGTCAGAA AAAGTATTGA GCAGCATAAA
    CTGGGGACCA ATAAAACCCT GCTGAAGCAG GTACGAGTAG TTGGAGTTAC CTGTGCAGCT
    TGCCCATTCC CATGTATGAA TGATCTTAAA TTTCCTGTAG TTGTGTTGGA TGAGTGTAGT
    CAAATAACTG AACCGGCCTC TCTCCTTCCT ATTGCAAGGT TTGAGTGTGA AAAGCTGATT
    CTTGTTGGAG ATCCTAAACA GCTTTCTCCT ACTATTCAGG GTTCTGATGC AGCCCATGAA
    AATGGATTGG AACAAACTCT TTTTGATCGG CTTTGCTTAA TGGGCCATAA GCCAGTTCTG
    TTGAGAACCC AGTACCGTTG TCACCCTGCA ATCAGTGATA TCTCTAATGA TCTGTTTTAT
    GAAGGAAACC TTGTGGATGG CATTTCTGAA ACAGAGAGGG GCCCTTTATT GGAATGGCTC
    CCAACACTAT GTTTCTATAA CATTAAAGGA CCTGAACAGA TTGAAAGAGA TAACAGCTTT
    CATAATGTGG CAGAAGCTGC TTTTACACTC AAGCTGATTC AATCACTGAT TGCGAGTGGG
    ATAGCGGGCT CTATGATTGG GGTGATAACA TTATACAAAT CCCAGATGTA CAAGCTTTGT
    CATTTACTCA GTGCCGTGGA CTTTGACAAT CCTGATATTA AAGCTGTGCA GGTATCCACA
    GTAGATGCTT TTCAGGGAGC TGAAAAGGAG ATCATTATTC TGTCCTGTGT AAGGACAAGA
    CAAGTAGGAT TTATTGATTC AGAAAAAAGA ATGAATGTTG CACTGACCAG AGGAAGGAGG
    CATTTATTGA TCGTGGGAAA TTTAGCCTGT TTGCGGAAAA ATCGACTCTG GGGACGTGTA
    ATCCAACACT GTGAAGGAAG GAGAGATGGA TTACAACATG CAAGCCAGTG TGAACCACAG
    CTGAACCATC TTCTTAAAGA GTATTTTGAA AAACGAGCAG AAGAAAAGCA GAAAAAAAGT
    GAAAAAGATA AATCTAAAGA TAAATCACAT TCACAAAAAG ATATGGTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965536.1
    CDS48..6578
    Translation

    Target ORF information:

    RefSeq Version XM_014110061.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris zinc finger, GRF-type containing 1 (ZGRF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110061.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGAAAGCC AGGAATTTAT TGTACTGTAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
    TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAGTTTT ATATGATGAC
    AAAGGAGAAT GTTTGGAGAG TATATTTCTT AAGTGCCTTG AGGTGAAGCC TGGAGATGAC
    TTAGAAGGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATTGCT
    ATTAAACCGG ATGTCATTAA AGAAACATCA GAGTTAAATC CAAGGAAATT TATATCCTCT
    GGGCGGTCCC TTGGATGTCA GCCTGCTGGT TTAAAAAGGA AGTTTACTGG TTTTCAAGGA
    CCACGTCAAG TGCCAAGGAA AATGGTTATT ACAGAAAATG ATGAATCAGC TGCATCACCT
    GAGGCTAAGA AACCTGGCCC TATTTTTCTT TCTCCCTTCT ACAACACACC TCCTTTGTTT
    TCTACTGCTG GCAAGAAAGA TACAAATAAT ATGCCAACAG ACCCTGAGAA CAGTGTCATT
    TACAAGAACA GAGAGAAAAA TGGCTTATAT TATTCTTCTG TTGTCTCTAC TCCATCCTTT
    AAGAATAACC CAGAAATGCT ATGTGAAGAA AATTATTTTT GCTCACCTGT CTATTCTGTA
    AATAAGCATT CAGATTCTTT ACTATCCAAT GAGGCCATAA AAAAAGATGG TTTGACATCT
    CACTGTTTAG GAGTTTCAGA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTAAAG
    TCCAAATCAA CTAGTATGGG TAAAGAACTA GATTCTGGGA TTACAGAACA TTTCCCTCAG
    ATACAACTAC AAGAAAGTGT AAACATTCCT TCTAAACCAA AGTGCATAAT TGAACAAGAC
    AAGTGTGGTG AGGTGAAGAG CACAGACAAT TTATACTACC AGCATCAATC GGAAAATACT
    ATGAGCTATA AAAGCAGATG GGCCATGTAT TTATCCTCAC AGAGTTCACC TGTACGTTCT
    TCTACTGAAG ATGGAAATAA TATAGAAAGG AAACCCAAAA CTCAGGGAGA TAATGTAAAT
    TTAAATTTGA AAGACCTTGT ACAAAAAAGG ATACAGTTTT TTGAAACATG TACTGAAAAG
    GGTAAAAACT ATAATGAAGA CAAGCCAGTA GGTGATAATG ATCAATCTTG CGATCAGGAA
    GTAAAATTGG AAATTCCTTC ATTCTGTAAA AGCAACAGCT TACCATTTAC CTGTAGCAGT
    GTAGAGAATG ATGGCTTATT ATCAGAATCT GACATTCAAA AAAATAATAA AATACCTGTT
    AGTCAGGATG ACCAGATGTG TCTAAAAGGA TCAATTCTCA TTAGAGAAAA TGCTCAGAAG
    ATAAATATAT GTGGAACCCC AGAAAAGTAT GACCAACCCA TGTCGTCCCT GCCAGAATCT
    GAACATCTAC AAATTGAGTC TTCTACAAGT AACAATTCTA GGATCTCTGA TGACTTTACC
    AATATGCTTT CTAAAAGTAA TACTGATAAT GAAAATCTTA ATAGTATTCC TGAACCAATG
    AGTAATGTAA CACAGCCACT TGTGGAGGTG ACTTTTAATC TGAACAATTT TGAAACCAGT
    GACTCTGAGG AGGAATCACA AGAAAATAAC AAAATTTCCC AGGATTCAGA GGGTTGGGTA
    AAGGAAACTC TGGTTAATGA TGGCAGTTCA AGTGTTCAAA AGAGATGTGA AAATATAAGC
    TGTAAGAAAG TTGGCAGTGA ACATTTGTCT CATTTAACAT CTATGGAGGA TAAGCCTACA
    GAGACATTTC CTAAAGAGAC TCTGCTGTCA CAGTTTTGTG ATAAAGCGTG TATGGGTTTT
    GACACAGGAC CTTGGAAAAC TGGAAACAGA AAAGAAATAG AAGAGTATAA TGACACATTA
    AGCAGTTTTG ATTCATCTTT AGAGTGGACT GATGATGTAT ATGTAGATAA TAATGAAGAT
    GCTAATAAAT CTATACAAAA AGTCAGAATT AACTATGATA TTGCTTCACT ACCGAGTAAG
    TCTAAAGGTA TAAGCTCAAA TTTACATATT CCTCAGTTAA ACATAGCCAC TAATCAGATT
    CCTAAAAACA GCCTATTCTC GGAACAGTCT GGGCTTAAGC CTTTTATAAT GGGAAATAAA
    TTAGACAAAA ATGATGAACA GGTTTTACTG TCAACTTCTA GCAGTGACAT CAGTATCCAA
    CAATTAAATG TTAATCAGAA TCACTCTGAA GAACCTGTTG CTCTTGGTAA ATCAAATACC
    CAAGTTTCCA ATTCTTTGTT GTACCTTCTC GGAGAAAAGC ATCCTATTTC CAAAGAAACA
    GAAGCGTGTA TTCCTGAATC CGAAGATTTG GGAAGGATTA GAAGTTTAGA CCATGACCAT
    ATTGAAGTAG AACCTGCTGG AGAAAGCAAA CAACATTGGA ATAGTCCTAG GAATCCTTCA
    GAACTCTCTG GATTAATAAA TAACATTTCC CTTTTAAAGT CATTGTCTGA ACATAGTACA
    GCTTTAGAAG GCTTGGAAAT ATTGAAAAAG AAAAATACTA CCTTTAAACA ACAAGGAACT
    CTACAGACAT ATAATCCAGA TAGTAATTCT GAAGCTAGGA AACGATCGAC TGCAGAAGTT
    TTGCAAGCTT TACCAAAGTT GCCTCCTCTG AACCAGGATT CTCAACAGAT TATAAAAGAA
    AATGAAGTTG AACCAAGTAA ACCACTTCAG TCTTTGCAGT TCTTTTCCTT TGGAGATAAA
    GAGACTGCTT TTCTAGATGT TATGCCTAAA CAATCAGAGA GAAAAACCTG TGACCCTAAG
    CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA GGATCTGCTA CTAGTGCTGT AATGATGAGA
    GGACACAGCT CACAGCTAGA ATGCGGTCAA TTTCCAGATA GTATTGAGTA TGAAAACTGC
    ACAAGAGACA CTTGTTTATT GACACCAAAA TTGACCAGTG CTTGTATGCA GATTGATTTC
    CTGCAGTTCC CAGATGAAAG AAACATTCCA AAAGAAGATA TTAGCTTTAA TATTGAAGAC
    AATTTTCAAG TGAGAGCTGA GTCAAATAAG GACTCAATTG AAAATGACTT GATTTTGGAT
    TTCTCTCCTA AAAATTCAGA ACATTTCTAT CATTATTTGG ATTCTAGTTC TAAATCAGCA
    GAGAAGACTT CATGGGAAGC AAATAAGGTA ACATCACCAG AACAGAAGAT CTCTACATTA
    AACCCTGATT CTACTTTTTC TTTGAACTCA CGAGATGAAG ACTTTGTGCT AGAATTCTCT
    GAGGAGTCCC TGAAAGAAAG AACTTTACCT GTTATCAAAA AATCCAGTTC TTTGGAGAAT
    AAGAACTTTC AAAGGCTTCC ATTAGTAAGT AGAACCCGAG TTCCACTAAC TACTTTGCCA
    CCTGCTGATT CAGATTCTTG TTCATACGTG ATCAACTGTG ACAGGCAAGA ATATTCTGGT
    TCCTCCCCCA TATTCAACTT ATGTGAAGAA TCAGGAGTTC TTTCATTTAG CTTGGGGCTT
    GAGGACCAAT GTGAAACTTC CTTCTGTAAA GAATCTAGAG AAGTGATTCC ACAGGACGTT
    TTGCTTCTCA ATAATATACC TACTCAAAGC AAGTGGCTGA AATATCAGAA CATACCCCAG
    TGCAACTTGA CTACTCCAGA CAGAGTTGAT AAGAAAGTAA CAGATGGTTT CTCTGGTAAA
    GTTATTTCTG GGACACATTT AAGTGATATA CGTGAAAGAC AGAGTGGTGC TGTGAATGAA
    AGCTCTTTAG ACTCTATATA TTTGCAAATG ATAAAAGGCA TGCTCTATCA ACAGCAGCAG
    GATTTTAGCT GTCAGGATTT GGTTTTCAGA AAGAAAGCAC TCTCATTGAA TTTAAAGCTG
    ACTTCCCAGA CTGAAAAAAT TCAAAACACG TTAAGAGAGT CTGCTGCCTG CTACATCTAC
    AGTGTAACAG GAGATTTACA GGAGATAAAT GCCTCTGAGC TATGCTTCCC AAGTGGGCAG
    AAAATAAAAT CTGCTTATCT TCCCCAAAGG CAGATTCATA TACCAGCTGT TTTTCAGTCT
    CCTGCTCATT ATAAGCAGAT TTTTACATCT TGTCTCATAG AACACTTAAA TATATTGTTG
    TTTGGGTTGG CACAGAGGTT GCACAAAGCT CTTTCAAAAG TTGACATATC ATTTTACACA
    TCATTAAAGG GAGAGAAACT AAAAAATGTA GAAAGTAATG TACCATCCTG CCATCATCAT
    CAACCTGCAA AACTTGTCAT GGTTAAAAAG GAAGGTCCAA ATAAGGGTCG TCTTTTTTAT
    ACCTGTGATG GACCCAAAGC TGACCGATGT AAATTTTTCA AGTGGCTTGA AGAAGTGACC
    CCAGGATATT TGACACAAGA AGAGTCTCTA CCTAGCATAG TTTTAAGTGA TATAAAGAGT
    ATTGGCTTAT ACTTAAGAAG TCAAAATATA CCCCTCTTTG AGGAATGTCA GCTTTTGGTG
    AGGAAAGGAT TTGATTTTCA AAGAAAACAG TATGGCAAAC TGAAGAAGTT TAATGCTGTA
    AATCCTGAAT TTTATAGTGA ACCTAAAAGC AAACTTTATC TTAAGCTGAG TCGGAGGGAA
    AGTTCTTCAA CTTACAGCAA AAATGATCTT TGGGTGGTTT CAAAAACATT GGACTTTGAA
    TTGGATACTT TTATTGCGTG TAGTGCTTTC TTTGGACCAT CATCTATCAA TGAGGTGGAG
    CTACTACCTT TGAAAGGTTA TTTCCCCTCT AACTGGCCCA CTAACATAGT GGTACATGCA
    TTGTTGGTTT GTAATGCTAG CACTGAGCTG ACAACTTTGA AAAACATTCA GGACTACTTT
    AATCCAGCTA CTCTACCTCT AACACAGTAC TTGTTAACAA TGTCTTCATC AACTATAGTT
    AGCAACAAGA GAGTCAATAA GAGAAAATTT ATCCCACCAG CCTTCTCAAA TATCAATACA
    AAATTTGAAC TTCTCAGCCT AGAAGCCACA TTACAGTTAG CTAGTGAATT AATTCAGGCA
    CACAACTTAA ATAAGGATCA AGCTACAGCT TTAATTCAGA TAGCTCAAAT GATGGCATCA
    CATGAAAATG CTGAAGAAGT GAAGAAAGTA CAAACCCATA CCCTCCCTAT CACAATCATA
    CATGGTGTTT TTGGAGCAGG AAAGAGTTAT TTGTTGGCAG TGGTGATTTT GTTTTTTGTA
    CAGCTATTTG AAAAGAGTGA AGTTCTCACA GTTGGAAATG TAAGACCTTG GAAACTTCTC
    ATTTCTTCTT CCACTAATGT AGCTGTGGAC AGAGTACTTC TTGGGCTTCT CAGTCTTGGT
    TTTGAAAAGT TCGTCAGAGT TGGGAGTGTC AGGAAGATTG CCAAGCCAAT TTTACCTTAT
    AGTTTGCATG CTGGCTCAGA AAATGAAAAT GAACAACTAA AAGAACTACA TGGACTAATG
    AAAGAAGACT TGACTCCTCT GGAAAGGGTC TATGTCAGAA AAAGTATTGA GCAGCATAAA
    CTGGGGACCA ATAAAACCCT GCTGAAGCAG GTACGAGTAG TTGGAGTTAC CTGTGCAGCT
    TGCCCATTCC CATGTATGAA TGATCTTAAA TTTCCTGTAG TTGTGTTGGA TGAGTGTAGT
    CAAATAACTG AACCGGCCTC TCTCCTTCCT ATTGCAAGGT TTGAGTGTGA AAAGCTGATT
    CTTGTTGGAG ATCCTAAACA GCTTTCTCCT ACTATTCAGG GTTCTGATGC AGCCCATGAA
    AATGGATTGG AACAAACTCT TTTTGATCGG CTTTGCTTAA TGGGCCATAA GCCAGTTCTG
    TTGAGAACCC AGTACCGTTG TCACCCTGCA ATCAGTGATA TCTCTAATGA TCTGTTTTAT
    GAAGGAAACC TTGTGGATGG CATTTCTGAA ACAGAGAGGG GCCCTTTATT GGAATGGCTC
    CCAACACTAT GTTTCTATAA CATTAAAGGA CCTGAACAGA TTGAAAGAGA TAACAGCTTT
    CATAATGTGG CAGAAGCTGC TTTTACACTC AAGCTGATTC AATCACTGAT TGCGAGTGGG
    ATAGCGGGCT CTATGATTGG GGTGATAACA TTATACAAAT CCCAGATGTA CAAGCTTTGT
    CATTTACTCA GTGCCGTGGA CTTTGACAAT CCTGATATTA AAGCTGTGCA GGTATCCACA
    GTAGATGCTT TTCAGGGAGC TGAAAAGGAG ATCATTATTC TGTCCTGTGT AAGGACAAGA
    CAAGTAGGAT TTATTGATTC AGAAAAAAGA ATGAATGTTG CACTGACCAG AGGAAGGAGG
    CATTTATTGA TCGTGGGAAA TTTAGCCTGT TTGCGGAAAA ATCGACTCTG GGGACGTGTA
    ATCCAACACT GTGAAGGAAG GAGAGATGGA TTACAACATG CAAGCCAGTG TGAACCACAG
    CTGAACCATC TTCTTAAAGA GTATTTTGAA AAACGAGCAG AAGAAAAGCA GAAAAAAAGT
    GAAAAAGATA AATCTAAAGA TAAATCACAT TCACAAAAAG ATATGGTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44624
    Clone ID Related Accession (Same CDS sequence) XM_014110061.2 , XM_014110061.1 , XM_022413411.1
    Accession Version XM_022413411.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein ZGRF1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGAAAGCC AGGAATTTAT TGTACTGTAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
    TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAGTTTT ATATGATGAC
    AAAGGAGAAT GTTTGGAGAG TATATTTCTT AAGTGCCTTG AGGTGAAGCC TGGAGATGAC
    TTAGAAGGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATTGCT
    ATTAAACCGG ATGTCATTAA AGAAACATCA GAGTTAAATC CAAGGAAATT TATATCCTCT
    GGGCGGTCCC TTGGATGTCA GCCTGCTGGT TTAAAAAGGA AGTTTACTGG TTTTCAAGGA
    CCACGTCAAG TGCCAAGGAA AATGGTTATT ACAGAAAATG ATGAATCAGC TGCATCACCT
    GAGGCTAAGA AACCTGGCCC TATTTTTCTT TCTCCCTTCT ACAACACACC TCCTTTGTTT
    TCTACTGCTG GCAAGAAAGA TACAAATAAT ATGCCAACAG ACCCTGAGAA CAGTGTCATT
    TACAAGAACA GAGAGAAAAA TGGCTTATAT TATTCTTCTG TTGTCTCTAC TCCATCCTTT
    AAGAATAACC CAGAAATGCT ATGTGAAGAA AATTATTTTT GCTCACCTGT CTATTCTGTA
    AATAAGCATT CAGATTCTTT ACTATCCAAT GAGGCCATAA AAAAAGATGG TTTGACATCT
    CACTGTTTAG GAGTTTCAGA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTAAAG
    TCCAAATCAA CTAGTATGGG TAAAGAACTA GATTCTGGGA TTACAGAACA TTTCCCTCAG
    ATACAACTAC AAGAAAGTGT AAACATTCCT TCTAAACCAA AGTGCATAAT TGAACAAGAC
    AAGTGTGGTG AGGTGAAGAG CACAGACAAT TTATACTACC AGCATCAATC GGAAAATACT
    ATGAGCTATA AAAGCAGATG GGCCATGTAT TTATCCTCAC AGAGTTCACC TGTACGTTCT
    TCTACTGAAG ATGGAAATAA TATAGAAAGG AAACCCAAAA CTCAGGGAGA TAATGTAAAT
    TTAAATTTGA AAGACCTTGT ACAAAAAAGG ATACAGTTTT TTGAAACATG TACTGAAAAG
    GGTAAAAACT ATAATGAAGA CAAGCCAGTA GGTGATAATG ATCAATCTTG CGATCAGGAA
    GTAAAATTGG AAATTCCTTC ATTCTGTAAA AGCAACAGCT TACCATTTAC CTGTAGCAGT
    GTAGAGAATG ATGGCTTATT ATCAGAATCT GACATTCAAA AAAATAATAA AATACCTGTT
    AGTCAGGATG ACCAGATGTG TCTAAAAGGA TCAATTCTCA TTAGAGAAAA TGCTCAGAAG
    ATAAATATAT GTGGAACCCC AGAAAAGTAT GACCAACCCA TGTCGTCCCT GCCAGAATCT
    GAACATCTAC AAATTGAGTC TTCTACAAGT AACAATTCTA GGATCTCTGA TGACTTTACC
    AATATGCTTT CTAAAAGTAA TACTGATAAT GAAAATCTTA ATAGTATTCC TGAACCAATG
    AGTAATGTAA CACAGCCACT TGTGGAGGTG ACTTTTAATC TGAACAATTT TGAAACCAGT
    GACTCTGAGG AGGAATCACA AGAAAATAAC AAAATTTCCC AGGATTCAGA GGGTTGGGTA
    AAGGAAACTC TGGTTAATGA TGGCAGTTCA AGTGTTCAAA AGAGATGTGA AAATATAAGC
    TGTAAGAAAG TTGGCAGTGA ACATTTGTCT CATTTAACAT CTATGGAGGA TAAGCCTACA
    GAGACATTTC CTAAAGAGAC TCTGCTGTCA CAGTTTTGTG ATAAAGCGTG TATGGGTTTT
    GACACAGGAC CTTGGAAAAC TGGAAACAGA AAAGAAATAG AAGAGTATAA TGACACATTA
    AGCAGTTTTG ATTCATCTTT AGAGTGGACT GATGATGTAT ATGTAGATAA TAATGAAGAT
    GCTAATAAAT CTATACAAAA AGTCAGAATT AACTATGATA TTGCTTCACT ACCGAGTAAG
    TCTAAAGGTA TAAGCTCAAA TTTACATATT CCTCAGTTAA ACATAGCCAC TAATCAGATT
    CCTAAAAACA GCCTATTCTC GGAACAGTCT GGGCTTAAGC CTTTTATAAT GGGAAATAAA
    TTAGACAAAA ATGATGAACA GGTTTTACTG TCAACTTCTA GCAGTGACAT CAGTATCCAA
    CAATTAAATG TTAATCAGAA TCACTCTGAA GAACCTGTTG CTCTTGGTAA ATCAAATACC
    CAAGTTTCCA ATTCTTTGTT GTACCTTCTC GGAGAAAAGC ATCCTATTTC CAAAGAAACA
    GAAGCGTGTA TTCCTGAATC CGAAGATTTG GGAAGGATTA GAAGTTTAGA CCATGACCAT
    ATTGAAGTAG AACCTGCTGG AGAAAGCAAA CAACATTGGA ATAGTCCTAG GAATCCTTCA
    GAACTCTCTG GATTAATAAA TAACATTTCC CTTTTAAAGT CATTGTCTGA ACATAGTACA
    GCTTTAGAAG GCTTGGAAAT ATTGAAAAAG AAAAATACTA CCTTTAAACA ACAAGGAACT
    CTACAGACAT ATAATCCAGA TAGTAATTCT GAAGCTAGGA AACGATCGAC TGCAGAAGTT
    TTGCAAGCTT TACCAAAGTT GCCTCCTCTG AACCAGGATT CTCAACAGAT TATAAAAGAA
    AATGAAGTTG AACCAAGTAA ACCACTTCAG TCTTTGCAGT TCTTTTCCTT TGGAGATAAA
    GAGACTGCTT TTCTAGATGT TATGCCTAAA CAATCAGAGA GAAAAACCTG TGACCCTAAG
    CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA GGATCTGCTA CTAGTGCTGT AATGATGAGA
    GGACACAGCT CACAGCTAGA ATGCGGTCAA TTTCCAGATA GTATTGAGTA TGAAAACTGC
    ACAAGAGACA CTTGTTTATT GACACCAAAA TTGACCAGTG CTTGTATGCA GATTGATTTC
    CTGCAGTTCC CAGATGAAAG AAACATTCCA AAAGAAGATA TTAGCTTTAA TATTGAAGAC
    AATTTTCAAG TGAGAGCTGA GTCAAATAAG GACTCAATTG AAAATGACTT GATTTTGGAT
    TTCTCTCCTA AAAATTCAGA ACATTTCTAT CATTATTTGG ATTCTAGTTC TAAATCAGCA
    GAGAAGACTT CATGGGAAGC AAATAAGGTA ACATCACCAG AACAGAAGAT CTCTACATTA
    AACCCTGATT CTACTTTTTC TTTGAACTCA CGAGATGAAG ACTTTGTGCT AGAATTCTCT
    GAGGAGTCCC TGAAAGAAAG AACTTTACCT GTTATCAAAA AATCCAGTTC TTTGGAGAAT
    AAGAACTTTC AAAGGCTTCC ATTAGTAAGT AGAACCCGAG TTCCACTAAC TACTTTGCCA
    CCTGCTGATT CAGATTCTTG TTCATACGTG ATCAACTGTG ACAGGCAAGA ATATTCTGGT
    TCCTCCCCCA TATTCAACTT ATGTGAAGAA TCAGGAGTTC TTTCATTTAG CTTGGGGCTT
    GAGGACCAAT GTGAAACTTC CTTCTGTAAA GAATCTAGAG AAGTGATTCC ACAGGACGTT
    TTGCTTCTCA ATAATATACC TACTCAAAGC AAGTGGCTGA AATATCAGAA CATACCCCAG
    TGCAACTTGA CTACTCCAGA CAGAGTTGAT AAGAAAGTAA CAGATGGTTT CTCTGGTAAA
    GTTATTTCTG GGACACATTT AAGTGATATA CGTGAAAGAC AGAGTGGTGC TGTGAATGAA
    AGCTCTTTAG ACTCTATATA TTTGCAAATG ATAAAAGGCA TGCTCTATCA ACAGCAGCAG
    GATTTTAGCT GTCAGGATTT GGTTTTCAGA AAGAAAGCAC TCTCATTGAA TTTAAAGCTG
    ACTTCCCAGA CTGAAAAAAT TCAAAACACG TTAAGAGAGT CTGCTGCCTG CTACATCTAC
    AGTGTAACAG GAGATTTACA GGAGATAAAT GCCTCTGAGC TATGCTTCCC AAGTGGGCAG
    AAAATAAAAT CTGCTTATCT TCCCCAAAGG CAGATTCATA TACCAGCTGT TTTTCAGTCT
    CCTGCTCATT ATAAGCAGAT TTTTACATCT TGTCTCATAG AACACTTAAA TATATTGTTG
    TTTGGGTTGG CACAGAGGTT GCACAAAGCT CTTTCAAAAG TTGACATATC ATTTTACACA
    TCATTAAAGG GAGAGAAACT AAAAAATGTA GAAAGTAATG TACCATCCTG CCATCATCAT
    CAACCTGCAA AACTTGTCAT GGTTAAAAAG GAAGGTCCAA ATAAGGGTCG TCTTTTTTAT
    ACCTGTGATG GACCCAAAGC TGACCGATGT AAATTTTTCA AGTGGCTTGA AGAAGTGACC
    CCAGGATATT TGACACAAGA AGAGTCTCTA CCTAGCATAG TTTTAAGTGA TATAAAGAGT
    ATTGGCTTAT ACTTAAGAAG TCAAAATATA CCCCTCTTTG AGGAATGTCA GCTTTTGGTG
    AGGAAAGGAT TTGATTTTCA AAGAAAACAG TATGGCAAAC TGAAGAAGTT TAATGCTGTA
    AATCCTGAAT TTTATAGTGA ACCTAAAAGC AAACTTTATC TTAAGCTGAG TCGGAGGGAA
    AGTTCTTCAA CTTACAGCAA AAATGATCTT TGGGTGGTTT CAAAAACATT GGACTTTGAA
    TTGGATACTT TTATTGCGTG TAGTGCTTTC TTTGGACCAT CATCTATCAA TGAGGTGGAG
    CTACTACCTT TGAAAGGTTA TTTCCCCTCT AACTGGCCCA CTAACATAGT GGTACATGCA
    TTGTTGGTTT GTAATGCTAG CACTGAGCTG ACAACTTTGA AAAACATTCA GGACTACTTT
    AATCCAGCTA CTCTACCTCT AACACAGTAC TTGTTAACAA TGTCTTCATC AACTATAGTT
    AGCAACAAGA GAGTCAATAA GAGAAAATTT ATCCCACCAG CCTTCTCAAA TATCAATACA
    AAATTTGAAC TTCTCAGCCT AGAAGCCACA TTACAGTTAG CTAGTGAATT AATTCAGGCA
    CACAACTTAA ATAAGGATCA AGCTACAGCT TTAATTCAGA TAGCTCAAAT GATGGCATCA
    CATGAAAATG CTGAAGAAGT GAAGAAAGTA CAAACCCATA CCCTCCCTAT CACAATCATA
    CATGGTGTTT TTGGAGCAGG AAAGAGTTAT TTGTTGGCAG TGGTGATTTT GTTTTTTGTA
    CAGCTATTTG AAAAGAGTGA AGTTCTCACA GTTGGAAATG TAAGACCTTG GAAACTTCTC
    ATTTCTTCTT CCACTAATGT AGCTGTGGAC AGAGTACTTC TTGGGCTTCT CAGTCTTGGT
    TTTGAAAAGT TCGTCAGAGT TGGGAGTGTC AGGAAGATTG CCAAGCCAAT TTTACCTTAT
    AGTTTGCATG CTGGCTCAGA AAATGAAAAT GAACAACTAA AAGAACTACA TGGACTAATG
    AAAGAAGACT TGACTCCTCT GGAAAGGGTC TATGTCAGAA AAAGTATTGA GCAGCATAAA
    CTGGGGACCA ATAAAACCCT GCTGAAGCAG GTACGAGTAG TTGGAGTTAC CTGTGCAGCT
    TGCCCATTCC CATGTATGAA TGATCTTAAA TTTCCTGTAG TTGTGTTGGA TGAGTGTAGT
    CAAATAACTG AACCGGCCTC TCTCCTTCCT ATTGCAAGGT TTGAGTGTGA AAAGCTGATT
    CTTGTTGGAG ATCCTAAACA GCTTTCTCCT ACTATTCAGG GTTCTGATGC AGCCCATGAA
    AATGGATTGG AACAAACTCT TTTTGATCGG CTTTGCTTAA TGGGCCATAA GCCAGTTCTG
    TTGAGAACCC AGTACCGTTG TCACCCTGCA ATCAGTGATA TCTCTAATGA TCTGTTTTAT
    GAAGGAAACC TTGTGGATGG CATTTCTGAA ACAGAGAGGG GCCCTTTATT GGAATGGCTC
    CCAACACTAT GTTTCTATAA CATTAAAGGA CCTGAACAGA TTGAAAGAGA TAACAGCTTT
    CATAATGTGG CAGAAGCTGC TTTTACACTC AAGCTGATTC AATCACTGAT TGCGAGTGGG
    ATAGCGGGCT CTATGATTGG GGTGATAACA TTATACAAAT CCCAGATGTA CAAGCTTTGT
    CATTTACTCA GTGCCGTGGA CTTTGACAAT CCTGATATTA AAGCTGTGCA GGTATCCACA
    GTAGATGCTT TTCAGGGAGC TGAAAAGGAG ATCATTATTC TGTCCTGTGT AAGGACAAGA
    CAAGTAGGAT TTATTGATTC AGAAAAAAGA ATGAATGTTG CACTGACCAG AGGAAGGAGG
    CATTTATTGA TCGTGGGAAA TTTAGCCTGT TTGCGGAAAA ATCGACTCTG GGGACGTGTA
    ATCCAACACT GTGAAGGAAG GAGAGATGGA TTACAACATG CAAGCCAGTG TGAACCACAG
    CTGAACCATC TTCTTAAAGA GTATTTTGAA AAACGAGCAG AAGAAAAGCA GAAAAAAAGT
    GAAAAAGATA AATCTAAAGA TAAATCACAT TCACAAAAAG ATATGGTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269119.1
    CDS405..6935
    Translation

    Target ORF information:

    RefSeq Version XM_022413411.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris zinc finger GRF-type containing 1 (ZGRF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413411.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGAAAGCC AGGAATTTAT TGTACTGTAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
    TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAGTTTT ATATGATGAC
    AAAGGAGAAT GTTTGGAGAG TATATTTCTT AAGTGCCTTG AGGTGAAGCC TGGAGATGAC
    TTAGAAGGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATTGCT
    ATTAAACCGG ATGTCATTAA AGAAACATCA GAGTTAAATC CAAGGAAATT TATATCCTCT
    GGGCGGTCCC TTGGATGTCA GCCTGCTGGT TTAAAAAGGA AGTTTACTGG TTTTCAAGGA
    CCACGTCAAG TGCCAAGGAA AATGGTTATT ACAGAAAATG ATGAATCAGC TGCATCACCT
    GAGGCTAAGA AACCTGGCCC TATTTTTCTT TCTCCCTTCT ACAACACACC TCCTTTGTTT
    TCTACTGCTG GCAAGAAAGA TACAAATAAT ATGCCAACAG ACCCTGAGAA CAGTGTCATT
    TACAAGAACA GAGAGAAAAA TGGCTTATAT TATTCTTCTG TTGTCTCTAC TCCATCCTTT
    AAGAATAACC CAGAAATGCT ATGTGAAGAA AATTATTTTT GCTCACCTGT CTATTCTGTA
    AATAAGCATT CAGATTCTTT ACTATCCAAT GAGGCCATAA AAAAAGATGG TTTGACATCT
    CACTGTTTAG GAGTTTCAGA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTAAAG
    TCCAAATCAA CTAGTATGGG TAAAGAACTA GATTCTGGGA TTACAGAACA TTTCCCTCAG
    ATACAACTAC AAGAAAGTGT AAACATTCCT TCTAAACCAA AGTGCATAAT TGAACAAGAC
    AAGTGTGGTG AGGTGAAGAG CACAGACAAT TTATACTACC AGCATCAATC GGAAAATACT
    ATGAGCTATA AAAGCAGATG GGCCATGTAT TTATCCTCAC AGAGTTCACC TGTACGTTCT
    TCTACTGAAG ATGGAAATAA TATAGAAAGG AAACCCAAAA CTCAGGGAGA TAATGTAAAT
    TTAAATTTGA AAGACCTTGT ACAAAAAAGG ATACAGTTTT TTGAAACATG TACTGAAAAG
    GGTAAAAACT ATAATGAAGA CAAGCCAGTA GGTGATAATG ATCAATCTTG CGATCAGGAA
    GTAAAATTGG AAATTCCTTC ATTCTGTAAA AGCAACAGCT TACCATTTAC CTGTAGCAGT
    GTAGAGAATG ATGGCTTATT ATCAGAATCT GACATTCAAA AAAATAATAA AATACCTGTT
    AGTCAGGATG ACCAGATGTG TCTAAAAGGA TCAATTCTCA TTAGAGAAAA TGCTCAGAAG
    ATAAATATAT GTGGAACCCC AGAAAAGTAT GACCAACCCA TGTCGTCCCT GCCAGAATCT
    GAACATCTAC AAATTGAGTC TTCTACAAGT AACAATTCTA GGATCTCTGA TGACTTTACC
    AATATGCTTT CTAAAAGTAA TACTGATAAT GAAAATCTTA ATAGTATTCC TGAACCAATG
    AGTAATGTAA CACAGCCACT TGTGGAGGTG ACTTTTAATC TGAACAATTT TGAAACCAGT
    GACTCTGAGG AGGAATCACA AGAAAATAAC AAAATTTCCC AGGATTCAGA GGGTTGGGTA
    AAGGAAACTC TGGTTAATGA TGGCAGTTCA AGTGTTCAAA AGAGATGTGA AAATATAAGC
    TGTAAGAAAG TTGGCAGTGA ACATTTGTCT CATTTAACAT CTATGGAGGA TAAGCCTACA
    GAGACATTTC CTAAAGAGAC TCTGCTGTCA CAGTTTTGTG ATAAAGCGTG TATGGGTTTT
    GACACAGGAC CTTGGAAAAC TGGAAACAGA AAAGAAATAG AAGAGTATAA TGACACATTA
    AGCAGTTTTG ATTCATCTTT AGAGTGGACT GATGATGTAT ATGTAGATAA TAATGAAGAT
    GCTAATAAAT CTATACAAAA AGTCAGAATT AACTATGATA TTGCTTCACT ACCGAGTAAG
    TCTAAAGGTA TAAGCTCAAA TTTACATATT CCTCAGTTAA ACATAGCCAC TAATCAGATT
    CCTAAAAACA GCCTATTCTC GGAACAGTCT GGGCTTAAGC CTTTTATAAT GGGAAATAAA
    TTAGACAAAA ATGATGAACA GGTTTTACTG TCAACTTCTA GCAGTGACAT CAGTATCCAA
    CAATTAAATG TTAATCAGAA TCACTCTGAA GAACCTGTTG CTCTTGGTAA ATCAAATACC
    CAAGTTTCCA ATTCTTTGTT GTACCTTCTC GGAGAAAAGC ATCCTATTTC CAAAGAAACA
    GAAGCGTGTA TTCCTGAATC CGAAGATTTG GGAAGGATTA GAAGTTTAGA CCATGACCAT
    ATTGAAGTAG AACCTGCTGG AGAAAGCAAA CAACATTGGA ATAGTCCTAG GAATCCTTCA
    GAACTCTCTG GATTAATAAA TAACATTTCC CTTTTAAAGT CATTGTCTGA ACATAGTACA
    GCTTTAGAAG GCTTGGAAAT ATTGAAAAAG AAAAATACTA CCTTTAAACA ACAAGGAACT
    CTACAGACAT ATAATCCAGA TAGTAATTCT GAAGCTAGGA AACGATCGAC TGCAGAAGTT
    TTGCAAGCTT TACCAAAGTT GCCTCCTCTG AACCAGGATT CTCAACAGAT TATAAAAGAA
    AATGAAGTTG AACCAAGTAA ACCACTTCAG TCTTTGCAGT TCTTTTCCTT TGGAGATAAA
    GAGACTGCTT TTCTAGATGT TATGCCTAAA CAATCAGAGA GAAAAACCTG TGACCCTAAG
    CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA GGATCTGCTA CTAGTGCTGT AATGATGAGA
    GGACACAGCT CACAGCTAGA ATGCGGTCAA TTTCCAGATA GTATTGAGTA TGAAAACTGC
    ACAAGAGACA CTTGTTTATT GACACCAAAA TTGACCAGTG CTTGTATGCA GATTGATTTC
    CTGCAGTTCC CAGATGAAAG AAACATTCCA AAAGAAGATA TTAGCTTTAA TATTGAAGAC
    AATTTTCAAG TGAGAGCTGA GTCAAATAAG GACTCAATTG AAAATGACTT GATTTTGGAT
    TTCTCTCCTA AAAATTCAGA ACATTTCTAT CATTATTTGG ATTCTAGTTC TAAATCAGCA
    GAGAAGACTT CATGGGAAGC AAATAAGGTA ACATCACCAG AACAGAAGAT CTCTACATTA
    AACCCTGATT CTACTTTTTC TTTGAACTCA CGAGATGAAG ACTTTGTGCT AGAATTCTCT
    GAGGAGTCCC TGAAAGAAAG AACTTTACCT GTTATCAAAA AATCCAGTTC TTTGGAGAAT
    AAGAACTTTC AAAGGCTTCC ATTAGTAAGT AGAACCCGAG TTCCACTAAC TACTTTGCCA
    CCTGCTGATT CAGATTCTTG TTCATACGTG ATCAACTGTG ACAGGCAAGA ATATTCTGGT
    TCCTCCCCCA TATTCAACTT ATGTGAAGAA TCAGGAGTTC TTTCATTTAG CTTGGGGCTT
    GAGGACCAAT GTGAAACTTC CTTCTGTAAA GAATCTAGAG AAGTGATTCC ACAGGACGTT
    TTGCTTCTCA ATAATATACC TACTCAAAGC AAGTGGCTGA AATATCAGAA CATACCCCAG
    TGCAACTTGA CTACTCCAGA CAGAGTTGAT AAGAAAGTAA CAGATGGTTT CTCTGGTAAA
    GTTATTTCTG GGACACATTT AAGTGATATA CGTGAAAGAC AGAGTGGTGC TGTGAATGAA
    AGCTCTTTAG ACTCTATATA TTTGCAAATG ATAAAAGGCA TGCTCTATCA ACAGCAGCAG
    GATTTTAGCT GTCAGGATTT GGTTTTCAGA AAGAAAGCAC TCTCATTGAA TTTAAAGCTG
    ACTTCCCAGA CTGAAAAAAT TCAAAACACG TTAAGAGAGT CTGCTGCCTG CTACATCTAC
    AGTGTAACAG GAGATTTACA GGAGATAAAT GCCTCTGAGC TATGCTTCCC AAGTGGGCAG
    AAAATAAAAT CTGCTTATCT TCCCCAAAGG CAGATTCATA TACCAGCTGT TTTTCAGTCT
    CCTGCTCATT ATAAGCAGAT TTTTACATCT TGTCTCATAG AACACTTAAA TATATTGTTG
    TTTGGGTTGG CACAGAGGTT GCACAAAGCT CTTTCAAAAG TTGACATATC ATTTTACACA
    TCATTAAAGG GAGAGAAACT AAAAAATGTA GAAAGTAATG TACCATCCTG CCATCATCAT
    CAACCTGCAA AACTTGTCAT GGTTAAAAAG GAAGGTCCAA ATAAGGGTCG TCTTTTTTAT
    ACCTGTGATG GACCCAAAGC TGACCGATGT AAATTTTTCA AGTGGCTTGA AGAAGTGACC
    CCAGGATATT TGACACAAGA AGAGTCTCTA CCTAGCATAG TTTTAAGTGA TATAAAGAGT
    ATTGGCTTAT ACTTAAGAAG TCAAAATATA CCCCTCTTTG AGGAATGTCA GCTTTTGGTG
    AGGAAAGGAT TTGATTTTCA AAGAAAACAG TATGGCAAAC TGAAGAAGTT TAATGCTGTA
    AATCCTGAAT TTTATAGTGA ACCTAAAAGC AAACTTTATC TTAAGCTGAG TCGGAGGGAA
    AGTTCTTCAA CTTACAGCAA AAATGATCTT TGGGTGGTTT CAAAAACATT GGACTTTGAA
    TTGGATACTT TTATTGCGTG TAGTGCTTTC TTTGGACCAT CATCTATCAA TGAGGTGGAG
    CTACTACCTT TGAAAGGTTA TTTCCCCTCT AACTGGCCCA CTAACATAGT GGTACATGCA
    TTGTTGGTTT GTAATGCTAG CACTGAGCTG ACAACTTTGA AAAACATTCA GGACTACTTT
    AATCCAGCTA CTCTACCTCT AACACAGTAC TTGTTAACAA TGTCTTCATC AACTATAGTT
    AGCAACAAGA GAGTCAATAA GAGAAAATTT ATCCCACCAG CCTTCTCAAA TATCAATACA
    AAATTTGAAC TTCTCAGCCT AGAAGCCACA TTACAGTTAG CTAGTGAATT AATTCAGGCA
    CACAACTTAA ATAAGGATCA AGCTACAGCT TTAATTCAGA TAGCTCAAAT GATGGCATCA
    CATGAAAATG CTGAAGAAGT GAAGAAAGTA CAAACCCATA CCCTCCCTAT CACAATCATA
    CATGGTGTTT TTGGAGCAGG AAAGAGTTAT TTGTTGGCAG TGGTGATTTT GTTTTTTGTA
    CAGCTATTTG AAAAGAGTGA AGTTCTCACA GTTGGAAATG TAAGACCTTG GAAACTTCTC
    ATTTCTTCTT CCACTAATGT AGCTGTGGAC AGAGTACTTC TTGGGCTTCT CAGTCTTGGT
    TTTGAAAAGT TCGTCAGAGT TGGGAGTGTC AGGAAGATTG CCAAGCCAAT TTTACCTTAT
    AGTTTGCATG CTGGCTCAGA AAATGAAAAT GAACAACTAA AAGAACTACA TGGACTAATG
    AAAGAAGACT TGACTCCTCT GGAAAGGGTC TATGTCAGAA AAAGTATTGA GCAGCATAAA
    CTGGGGACCA ATAAAACCCT GCTGAAGCAG GTACGAGTAG TTGGAGTTAC CTGTGCAGCT
    TGCCCATTCC CATGTATGAA TGATCTTAAA TTTCCTGTAG TTGTGTTGGA TGAGTGTAGT
    CAAATAACTG AACCGGCCTC TCTCCTTCCT ATTGCAAGGT TTGAGTGTGA AAAGCTGATT
    CTTGTTGGAG ATCCTAAACA GCTTTCTCCT ACTATTCAGG GTTCTGATGC AGCCCATGAA
    AATGGATTGG AACAAACTCT TTTTGATCGG CTTTGCTTAA TGGGCCATAA GCCAGTTCTG
    TTGAGAACCC AGTACCGTTG TCACCCTGCA ATCAGTGATA TCTCTAATGA TCTGTTTTAT
    GAAGGAAACC TTGTGGATGG CATTTCTGAA ACAGAGAGGG GCCCTTTATT GGAATGGCTC
    CCAACACTAT GTTTCTATAA CATTAAAGGA CCTGAACAGA TTGAAAGAGA TAACAGCTTT
    CATAATGTGG CAGAAGCTGC TTTTACACTC AAGCTGATTC AATCACTGAT TGCGAGTGGG
    ATAGCGGGCT CTATGATTGG GGTGATAACA TTATACAAAT CCCAGATGTA CAAGCTTTGT
    CATTTACTCA GTGCCGTGGA CTTTGACAAT CCTGATATTA AAGCTGTGCA GGTATCCACA
    GTAGATGCTT TTCAGGGAGC TGAAAAGGAG ATCATTATTC TGTCCTGTGT AAGGACAAGA
    CAAGTAGGAT TTATTGATTC AGAAAAAAGA ATGAATGTTG CACTGACCAG AGGAAGGAGG
    CATTTATTGA TCGTGGGAAA TTTAGCCTGT TTGCGGAAAA ATCGACTCTG GGGACGTGTA
    ATCCAACACT GTGAAGGAAG GAGAGATGGA TTACAACATG CAAGCCAGTG TGAACCACAG
    CTGAACCATC TTCTTAAAGA GTATTTTGAA AAACGAGCAG AAGAAAAGCA GAAAAAAAGT
    GAAAAAGATA AATCTAAAGA TAAATCACAT TCACAAAAAG ATATGGTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.