ROBO2 cDNA ORF clone, Canis lupus familiaris(Dog)

The following ROBO2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ROBO2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe283879 XM_022413103.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe44453 XM_014109735.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe44454 XM_014109736.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe44455 XM_014109737.2
Latest version!
Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe44456 XM_014109738.2
Latest version!
Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe44457 XM_014109739.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe44458 XM_014109740.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe283873 XM_022413096.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe283874 XM_022413098.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe283875 XM_022413099.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe283876 XM_022413100.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe283877 XM_022413101.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe283878 XM_022413102.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe29283 XM_022413097.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe44449 XM_014109730.2
Latest version!
Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe44450 XM_014109732.2
Latest version!
Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe44451 XM_014109733.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe44452 XM_014109734.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe29287 XM_005638758.2
Latest version!
Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OCe44449 XM_014109730.1 Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe44456 XM_014109738.1 Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe44450 XM_014109732.1 Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe44455 XM_014109737.1 Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe283879
    Clone ID Related Accession (Same CDS sequence) XM_022413103.1
    Accession Version XM_022413103.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product roundabout homolog 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTTACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAAAAAGA AGGCAGCCAG
    AACTTGCTTT TTCCAAATCA ACCCCAGCAA CCCAACAGTA GATGCTCTGT TTCGCCAACT
    GGAGACCTCA CAATTACCAA CATTCAGCGC TCCGATGCGG GTTACTATAT CTGCCAGGCA
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AAGTTACTGA TGTTTTAACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCTGCAAACC AAACTCTAGC AGTTGATGGT
    ACAGCATTAC TGAAATGTAA AGCTACTGGG GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCTGGG TAGAGATTCA AGAGCAACTA TACAAGAACA AGGAACATTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGTACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGGGAGA CTTCGTGGAG TGCTGTGCTG GATGTGACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ACTTAAGTGA CCTACCAGGG CCACCATCCA AACCACAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAA CCAGGTACCC CTGGAGCCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC GGTGGCAAAC
    CATGTAAAGA CCACGCTCTA TACAGTGAGA GGATTGCGGC CCAATACGAT CTATTTATTC
    ATGGTCAGAG CTATCAACCC CCAAGGTCTA AGCGACCCAA GCCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ATAGACAAGT ACAGAAAGAA
    CTAGGAGATG TCATTGTCCG TCTTCATACT CCAGTTGTGT TGACTCCCAC CACTGTTCAG
    GTCACATGGA CGGTTGATCG CCAGCCCCAG TTTATTCAAG GCTACCGAGT GATGTATCGT
    CAGACCACAG GCCTGCAGGC TACGACAACA TGGCAGAATT TAGATGCCAA AGTCCCAACC
    GAGCGAAGTG CTGTCTTGGT CAACCTGAAA AAGGGAGTGA CTTATGAAAT TAAAGTTCGA
    CCATATTTTA ATGAGTTCCA AGGAATGGAC AGTGAATCTA AAATAGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCTCC ACAATCTGTC ACTGTGCTGA CAGTTGGAAG CCACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ATCAGAATGG AATCATCCAA
    GAATACAAGA TCTGGTGTCT GGGGAATGAA ACACGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GATCTGTGAT AATTGGTGGA TTGTTCCCAG GTATTCAGTA CCGGGTAGAG
    GTTGCAGCTA GTACAAGTGC AGGGGTTGGA GTAAAAAGTG AGCCGCAGCC GATAATAATT
    GGGGGACGTA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTTGTGA AGCAACCAGC CTTTATAGCT GGAATTGGTG GTGCCTGCTG GGTAATTTTG
    ATGGGTTTTA GCATCTGGTT GTATTGGCGA AGAAAGAAGA GAAAGGGACT CAGTAACTAT
    GCTGTTACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCTGGTCTT
    CTAAATGCTG GTGATCCCAG TTACCCATGG CTTGCTGATT CATGGCCAGC CACGAGCTTA
    CCAGTAAACA ATAGCAATAG TGGCCCAAAT GAGATTGGGA ATTTTGGACG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC GACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACAAC ACAGATCTTG CATTCCAATA GCATACATGA ATTGGCTGTC
    GATCTGCCGG ACCCACAGTG GAAGAGCTCA ATTCAGCAAA AAACGGACCT GATGGGCTTT
    GGATATTCTC TCCCTGATCA GAACAAGGGT AACAACGGTG GGAAAGGTGG AAAAAAGAAG
    AAAACTAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CGACTTGGGC CAATGTACCC
    CTACCTCCTC CCCCAGTCCA GCCCCTTCCT GGTACAGAGC TGGAACACTA TGCAGTGGAA
    CCGCAAGAAA ATGGGTATGA CAGTGATAGC TGGTGCCCGC CATTACCGGT ACAAACATAC
    TTACACCAGG GTATGGAAGA TGAACTGGAA GAAGATGATG ATCGTGTCCC AACACCTCCT
    GTCCGAGGCG TGGCTTCTTC TCCTGCTATT TCCTTCGGAC AGCAGTCCAC TGCAACTCTT
    ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAAG CTCACCTGGA TGAGTTGACA
    AGAGCCTATC AGTTTGATAT AGCGAAGCAA ACATGGCACA TTCAAAGCAA TAATCAACCT
    CCACAGCCCC CGGTTCCACC ATTAGGTTAC ATGTCCGGAG CCTTGATTTC CGATTTGGAA
    ACAGATGTTC CAGATGATGA CGCTGATGAT GAGGAGGAAG CTTTAGAAAT CCCCAGACCC
    CTGAGAGCAC TAGAGCAGAC ACCCGGATCC AGTATGGACA ATCTAGACAG CTCTGTGACA
    GGTTCAATGG TAAATGGCTG GGGCTCTGCA TCTGATGAGG ATCGTAACTT TTCTAGTCAT
    AGATCTAGTG TAGGTAGCTC CTCAGATGGC TCCATCTTTG CCAGCGGCAG CTTTGCACAA
    GCAGTGGTGG CAGCAGCAGA TAAAGCTGGT TTTAGGCTGG ATGGAACCAG CCTTACAAGA
    ACAGGCAAAG CTTTTACCTC CTCTCAAAGA CCTCGACCAA CCAGCCCATT TTCTACTGAC
    AGTAATACCA GTGCAGCCCT GAGTCAAAAT CAGAGGCCTC GGCCCACTAA AAAACACAAG
    GGAGGGCGAA TGGATCAACA ACCAGCATTG CCTCATCGAA GGGAAGGAAT GACAGATGAG
    GAAACATTGG TGCCATACAG CAAGCCCAGT TTCCCATCTC CGGGAGGTCA CAGCTCATCA
    GGAACAGCAT CTTCTAAAGG ATCCACTGGA CCTAGGAAAG CCGAGGCGTT GAGGGGAAGT
    CACCAACGCA ATGCCAGTGA CCTTCTTGAC ATAGGATATA TGGGCTCAAA TAGTCAAGGA
    CAGTTTACAG GTGAATTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268811.1
    CDS386..4786
    Translation

    Target ORF information:

    RefSeq Version XM_022413103.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413103.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTTACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAAAAAGA AGGCAGCCAG
    AACTTGCTTT TTCCAAATCA ACCCCAGCAA CCCAACAGTA GATGCTCTGT TTCGCCAACT
    GGAGACCTCA CAATTACCAA CATTCAGCGC TCCGATGCGG GTTACTATAT CTGCCAGGCA
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AAGTTACTGA TGTTTTAACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCTGCAAACC AAACTCTAGC AGTTGATGGT
    ACAGCATTAC TGAAATGTAA AGCTACTGGG GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCTGGG TAGAGATTCA AGAGCAACTA TACAAGAACA AGGAACATTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGTACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGGGAGA CTTCGTGGAG TGCTGTGCTG GATGTGACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ACTTAAGTGA CCTACCAGGG CCACCATCCA AACCACAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAA CCAGGTACCC CTGGAGCCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC GGTGGCAAAC
    CATGTAAAGA CCACGCTCTA TACAGTGAGA GGATTGCGGC CCAATACGAT CTATTTATTC
    ATGGTCAGAG CTATCAACCC CCAAGGTCTA AGCGACCCAA GCCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ATAGACAAGT ACAGAAAGAA
    CTAGGAGATG TCATTGTCCG TCTTCATACT CCAGTTGTGT TGACTCCCAC CACTGTTCAG
    GTCACATGGA CGGTTGATCG CCAGCCCCAG TTTATTCAAG GCTACCGAGT GATGTATCGT
    CAGACCACAG GCCTGCAGGC TACGACAACA TGGCAGAATT TAGATGCCAA AGTCCCAACC
    GAGCGAAGTG CTGTCTTGGT CAACCTGAAA AAGGGAGTGA CTTATGAAAT TAAAGTTCGA
    CCATATTTTA ATGAGTTCCA AGGAATGGAC AGTGAATCTA AAATAGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCTCC ACAATCTGTC ACTGTGCTGA CAGTTGGAAG CCACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ATCAGAATGG AATCATCCAA
    GAATACAAGA TCTGGTGTCT GGGGAATGAA ACACGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GATCTGTGAT AATTGGTGGA TTGTTCCCAG GTATTCAGTA CCGGGTAGAG
    GTTGCAGCTA GTACAAGTGC AGGGGTTGGA GTAAAAAGTG AGCCGCAGCC GATAATAATT
    GGGGGACGTA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTTGTGA AGCAACCAGC CTTTATAGCT GGAATTGGTG GTGCCTGCTG GGTAATTTTG
    ATGGGTTTTA GCATCTGGTT GTATTGGCGA AGAAAGAAGA GAAAGGGACT CAGTAACTAT
    GCTGTTACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCTGGTCTT
    CTAAATGCTG GTGATCCCAG TTACCCATGG CTTGCTGATT CATGGCCAGC CACGAGCTTA
    CCAGTAAACA ATAGCAATAG TGGCCCAAAT GAGATTGGGA ATTTTGGACG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC GACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACAAC ACAGATCTTG CATTCCAATA GCATACATGA ATTGGCTGTC
    GATCTGCCGG ACCCACAGTG GAAGAGCTCA ATTCAGCAAA AAACGGACCT GATGGGCTTT
    GGATATTCTC TCCCTGATCA GAACAAGGGT AACAACGGTG GGAAAGGTGG AAAAAAGAAG
    AAAACTAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CGACTTGGGC CAATGTACCC
    CTACCTCCTC CCCCAGTCCA GCCCCTTCCT GGTACAGAGC TGGAACACTA TGCAGTGGAA
    CCGCAAGAAA ATGGGTATGA CAGTGATAGC TGGTGCCCGC CATTACCGGT ACAAACATAC
    TTACACCAGG GTATGGAAGA TGAACTGGAA GAAGATGATG ATCGTGTCCC AACACCTCCT
    GTCCGAGGCG TGGCTTCTTC TCCTGCTATT TCCTTCGGAC AGCAGTCCAC TGCAACTCTT
    ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAAG CTCACCTGGA TGAGTTGACA
    AGAGCCTATC AGTTTGATAT AGCGAAGCAA ACATGGCACA TTCAAAGCAA TAATCAACCT
    CCACAGCCCC CGGTTCCACC ATTAGGTTAC ATGTCCGGAG CCTTGATTTC CGATTTGGAA
    ACAGATGTTC CAGATGATGA CGCTGATGAT GAGGAGGAAG CTTTAGAAAT CCCCAGACCC
    CTGAGAGCAC TAGAGCAGAC ACCCGGATCC AGTATGGACA ATCTAGACAG CTCTGTGACA
    GGTTCAATGG TAAATGGCTG GGGCTCTGCA TCTGATGAGG ATCGTAACTT TTCTAGTCAT
    AGATCTAGTG TAGGTAGCTC CTCAGATGGC TCCATCTTTG CCAGCGGCAG CTTTGCACAA
    GCAGTGGTGG CAGCAGCAGA TAAAGCTGGT TTTAGGCTGG ATGGAACCAG CCTTACAAGA
    ACAGGCAAAG CTTTTACCTC CTCTCAAAGA CCTCGACCAA CCAGCCCATT TTCTACTGAC
    AGTAATACCA GTGCAGCCCT GAGTCAAAAT CAGAGGCCTC GGCCCACTAA AAAACACAAG
    GGAGGGCGAA TGGATCAACA ACCAGCATTG CCTCATCGAA GGGAAGGAAT GACAGATGAG
    GAAACATTGG TGCCATACAG CAAGCCCAGT TTCCCATCTC CGGGAGGTCA CAGCTCATCA
    GGAACAGCAT CTTCTAAAGG ATCCACTGGA CCTAGGAAAG CCGAGGCGTT GAGGGGAAGT
    CACCAACGCA ATGCCAGTGA CCTTCTTGAC ATAGGATATA TGGGCTCAAA TAGTCAAGGA
    CAGTTTACAG GTGAATTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44453
    Clone ID Related Accession (Same CDS sequence) XM_014109735.1
    Accession Version XM_014109735.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4491bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product roundabout homolog 2 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTTACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAAAAAGA AGGCAGCCAG
    AACTTGCTTT TTCCAAATCA ACCCCAGCAA CCCAACAGTA GATGCTCTGT TTCGCCAACT
    GGAGACCTCA CAATTACCAA CATTCAGCGC TCCGATGCGG GTTACTATAT CTGCCAGGCA
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AAGTTACTGA TGTTTTAACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCTGCAAACC AAACTCTAGC AGTTGATGGT
    ACAGCATTAC TGAAATGTAA AGCTACTGGG GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCTGGG TAGAGATTCA AGAGCAACTA TACAAGAACA AGGAACATTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGTACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGGGAGA CTTCGTGGAG TGCTGTGCTG GATGTGACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ACTTAAGTGA CCTACCAGGG CCACCATCCA AACCACAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAA CCAGGTACCC CTGGAGCCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC GGTGGCAAAC
    CATGTAAAGA CCACGCTCTA TACAGTGAGA GGATTGCGGC CCAATACGAT CTATTTATTC
    ATGGTCAGAG CTATCAACCC CCAAGGTCTA AGCGACCCAA GCCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ATAGACAAGT ACAGAAAGAA
    CTAGGAGATG TCATTGTCCG TCTTCATACT CCAGTTGTGT TGACTCCCAC CACTGTTCAG
    GTCACATGGA CGGTTGATCG CCAGCCCCAG TTTATTCAAG GCTACCGAGT GATGTATCGT
    CAGACCACAG GCCTGCAGGC TACGACAACA TGGCAGAATT TAGATGCCAA AGTCCCAACC
    GAGCGAAGTG CTGTCTTGGT CAACCTGAAA AAGGGAGTGA CTTATGAAAT TAAAGTTCGA
    CCATATTTTA ATGAGTTCCA AGGAATGGAC AGTGAATCTA AAATAGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCTCC ACAATCTGTC ACTGTGCTGA CAGTTGGAAG CCACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ATCAGAATGG AATCATCCAA
    GAATACAAGA TCTGGTGTCT GGGGAATGAA ACACGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GATCTGTGAT AATTGGTGGA TTGTTCCCAG GTATTCAGTA CCGGGTAGAG
    GTTGCAGCTA GTACAAGTGC AGGGGTTGGA GTAAAAAGTG AGCCGCAGCC GATAATAATT
    GGGGGACGTA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTTGTGA AGCAACCAGC CTTTATAGCT GGAATTGGTG GTGCCTGCTG GGTAATTTTG
    ATGGGTTTTA GCATCTGGTT GTATTGGCGA AGAAAGAAGA GAAAGGGACT CAGTAACTAT
    GCTGTTACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCTGGTCTT
    CTAAATGCTG GTGATCCCAG TTACCCATGG CTTGCTGATT CATGGCCAGC CACGAGCTTA
    CCAGTAAACA ATAGCAATAG TGGCCCAAAT GAGATTGGGA ATTTTGGACG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC GACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACAAC ACAGATCTTG CATTCCAATA GCATACATGA ATTGGCTGTC
    GATCTGCCGG ACCCACAGTG GAAGAGCTCA ATTCAGCAAA AAACGGACCT GATGGGCTTT
    GGATATTCTC TCCCTGATCA GAACAAGGGT AACAACGGTG GGAAAGGTGG AAAAAAGAAG
    AAAACTAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CGACTTGGGC CAATGTACCC
    CTACCTCCTC CCCCAGTCCA GCCCCTTCCT GGTACAGAGC TGGAACACTA TGCAGTGGAA
    CCGCAAGAAA ATGGGTATGA CAGTGATAGC TGGTGCCCGC CATTACCGGT ACAAACATAC
    TTACACCAGG GTATGGAAGA TGAACTGGAA GAAGATGATG ATCGTGTCCC AACACCTCCT
    GTCCGAGGCG TGGCTTCTTC TCCTGCTATT TCCTTCGGAC AGCAGTCCAC TGCAACTCTT
    ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAAG CTCACCTGGA TGAGTTGACA
    AGAGCCTATC AGTTTGATAT AGCGAAGCAA ACATGGCACA TTCAAAGCAA TAATCAACCT
    CCACAGCCCC CGGTTCCACC ATTAGGTTAC ATGTCCGGAG CCTTGATTTC CGATTTGGAA
    ACAGATGTTC CAGATGATGA CGCTGATGAT GAGGAGGAAG CTTTAGAAAT CCCCAGACCC
    CTGAGAGCAC TAGAGCAGAC ACCCGGATCC AGTATGGACA ATCTAGACAG CTCTGTGACA
    GGCAAAGCTT TTACCTCCTC TCAAAGACCT CGACCAACCA GCCCATTTTC TACTGACAGT
    AATACCAGTG CAGCCCTGAG TCAAAATCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA
    GGGCGAATGG ATCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGATCTT
    CCACCACCAC CAGATCCCCC TCCAGGTCAG GGTTTAAGGC AGCAAATAGG CCCGAGCCAG
    CATGCTGGTA ACATGGAAAA CTCAACAGAG AGAAAAGGAA GCTCTCTGGA GACACCACAA
    GCATCCAACT TAGAAGACAC AAAGAGCTCA TTGGATTGTC CAGCTAGAAC GTCCCTAGAG
    TGGCAGCGAC AAACCCAAGA ATGGATAAAC TCCACAGAAC GCCAAGAAGA TATACGGAAA
    GCCCTACACA AACAAAGTGT TGGACCAGAG GAAACATTGG TGCCATACAG CAAGCCCAGT
    TTCCCATCTC CGGGAGGTCA CAGCTCATCA GGAACAGCAT CTTCTAAAGG ATCCACTGGA
    CCTAGGAAAG CCGAGGCGTT GAGGGGAAGT CACCAACGCA ATGCCAGTGA CCTTCTTGAC
    ATAGGATATA TGGGCTCAAA TAGTCAAGGA CAGTTTACAG GTGAATTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965210.1
    CDS584..5074
    Translation

    Target ORF information:

    RefSeq Version XM_014109735.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109735.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTTACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAAAAAGA AGGCAGCCAG
    AACTTGCTTT TTCCAAATCA ACCCCAGCAA CCCAACAGTA GATGCTCTGT TTCGCCAACT
    GGAGACCTCA CAATTACCAA CATTCAGCGC TCCGATGCGG GTTACTATAT CTGCCAGGCA
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AAGTTACTGA TGTTTTAACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCTGCAAACC AAACTCTAGC AGTTGATGGT
    ACAGCATTAC TGAAATGTAA AGCTACTGGG GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCTGGG TAGAGATTCA AGAGCAACTA TACAAGAACA AGGAACATTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGTACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGGGAGA CTTCGTGGAG TGCTGTGCTG GATGTGACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ACTTAAGTGA CCTACCAGGG CCACCATCCA AACCACAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAA CCAGGTACCC CTGGAGCCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC GGTGGCAAAC
    CATGTAAAGA CCACGCTCTA TACAGTGAGA GGATTGCGGC CCAATACGAT CTATTTATTC
    ATGGTCAGAG CTATCAACCC CCAAGGTCTA AGCGACCCAA GCCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ATAGACAAGT ACAGAAAGAA
    CTAGGAGATG TCATTGTCCG TCTTCATACT CCAGTTGTGT TGACTCCCAC CACTGTTCAG
    GTCACATGGA CGGTTGATCG CCAGCCCCAG TTTATTCAAG GCTACCGAGT GATGTATCGT
    CAGACCACAG GCCTGCAGGC TACGACAACA TGGCAGAATT TAGATGCCAA AGTCCCAACC
    GAGCGAAGTG CTGTCTTGGT CAACCTGAAA AAGGGAGTGA CTTATGAAAT TAAAGTTCGA
    CCATATTTTA ATGAGTTCCA AGGAATGGAC AGTGAATCTA AAATAGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCTCC ACAATCTGTC ACTGTGCTGA CAGTTGGAAG CCACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ATCAGAATGG AATCATCCAA
    GAATACAAGA TCTGGTGTCT GGGGAATGAA ACACGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GATCTGTGAT AATTGGTGGA TTGTTCCCAG GTATTCAGTA CCGGGTAGAG
    GTTGCAGCTA GTACAAGTGC AGGGGTTGGA GTAAAAAGTG AGCCGCAGCC GATAATAATT
    GGGGGACGTA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTTGTGA AGCAACCAGC CTTTATAGCT GGAATTGGTG GTGCCTGCTG GGTAATTTTG
    ATGGGTTTTA GCATCTGGTT GTATTGGCGA AGAAAGAAGA GAAAGGGACT CAGTAACTAT
    GCTGTTACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCTGGTCTT
    CTAAATGCTG GTGATCCCAG TTACCCATGG CTTGCTGATT CATGGCCAGC CACGAGCTTA
    CCAGTAAACA ATAGCAATAG TGGCCCAAAT GAGATTGGGA ATTTTGGACG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC GACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACAAC ACAGATCTTG CATTCCAATA GCATACATGA ATTGGCTGTC
    GATCTGCCGG ACCCACAGTG GAAGAGCTCA ATTCAGCAAA AAACGGACCT GATGGGCTTT
    GGATATTCTC TCCCTGATCA GAACAAGGGT AACAACGGTG GGAAAGGTGG AAAAAAGAAG
    AAAACTAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CGACTTGGGC CAATGTACCC
    CTACCTCCTC CCCCAGTCCA GCCCCTTCCT GGTACAGAGC TGGAACACTA TGCAGTGGAA
    CCGCAAGAAA ATGGGTATGA CAGTGATAGC TGGTGCCCGC CATTACCGGT ACAAACATAC
    TTACACCAGG GTATGGAAGA TGAACTGGAA GAAGATGATG ATCGTGTCCC AACACCTCCT
    GTCCGAGGCG TGGCTTCTTC TCCTGCTATT TCCTTCGGAC AGCAGTCCAC TGCAACTCTT
    ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAAG CTCACCTGGA TGAGTTGACA
    AGAGCCTATC AGTTTGATAT AGCGAAGCAA ACATGGCACA TTCAAAGCAA TAATCAACCT
    CCACAGCCCC CGGTTCCACC ATTAGGTTAC ATGTCCGGAG CCTTGATTTC CGATTTGGAA
    ACAGATGTTC CAGATGATGA CGCTGATGAT GAGGAGGAAG CTTTAGAAAT CCCCAGACCC
    CTGAGAGCAC TAGAGCAGAC ACCCGGATCC AGTATGGACA ATCTAGACAG CTCTGTGACA
    GGCAAAGCTT TTACCTCCTC TCAAAGACCT CGACCAACCA GCCCATTTTC TACTGACAGT
    AATACCAGTG CAGCCCTGAG TCAAAATCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA
    GGGCGAATGG ATCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGATCTT
    CCACCACCAC CAGATCCCCC TCCAGGTCAG GGTTTAAGGC AGCAAATAGG CCCGAGCCAG
    CATGCTGGTA ACATGGAAAA CTCAACAGAG AGAAAAGGAA GCTCTCTGGA GACACCACAA
    GCATCCAACT TAGAAGACAC AAAGAGCTCA TTGGATTGTC CAGCTAGAAC GTCCCTAGAG
    TGGCAGCGAC AAACCCAAGA ATGGATAAAC TCCACAGAAC GCCAAGAAGA TATACGGAAA
    GCCCTACACA AACAAAGTGT TGGACCAGAG GAAACATTGG TGCCATACAG CAAGCCCAGT
    TTCCCATCTC CGGGAGGTCA CAGCTCATCA GGAACAGCAT CTTCTAAAGG ATCCACTGGA
    CCTAGGAAAG CCGAGGCGTT GAGGGGAAGT CACCAACGCA ATGCCAGTGA CCTTCTTGAC
    ATAGGATATA TGGGCTCAAA TAGTCAAGGA CAGTTTACAG GTGAATTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44454
    Clone ID Related Accession (Same CDS sequence) XM_014109736.1
    Accession Version XM_014109736.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4344bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product roundabout homolog 2 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTTACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAAAAAGA AGGCAGCCAG
    AACTTGCTTT TTCCAAATCA ACCCCAGCAA CCCAACAGTA GATGCTCTGT TTCGCCAACT
    GGAGACCTCA CAATTACCAA CATTCAGCGC TCCGATGCGG GTTACTATAT CTGCCAGGCA
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AAGTTACTGA TGTTTTAACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCTGCAAACC AAACTCTAGC AGTTGATGGT
    ACAGCATTAC TGAAATGTAA AGCTACTGGG GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCTGGG TAGAGATTCA AGAGCAACTA TACAAGAACA AGGAACATTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGTACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGGGAGA CTTCGTGGAG TGCTGTGCTG GATGTGACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ACTTAAGTGA CCTACCAGGG CCACCATCCA AACCACAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAA CCAGGTACCC CTGGAGCCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC GGTGGCAAAC
    CATGTAAAGA CCACGCTCTA TACAGTGAGA GGATTGCGGC CCAATACGAT CTATTTATTC
    ATGGTCAGAG CTATCAACCC CCAAGGTCTA AGCGACCCAA GCCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ATAGACAAGT ACAGAAAGAA
    CTAGGAGATG TCATTGTCCG TCTTCATACT CCAGTTGTGT TGACTCCCAC CACTGTTCAG
    GTCACATGGA CGGTTGATCG CCAGCCCCAG TTTATTCAAG GCTACCGAGT GATGTATCGT
    CAGACCACAG GCCTGCAGGC TACGACAACA TGGCAGAATT TAGATGCCAA AGTCCCAACC
    GAGCGAAGTG CTGTCTTGGT CAACCTGAAA AAGGGAGTGA CTTATGAAAT TAAAGTTCGA
    CCATATTTTA ATGAGTTCCA AGGAATGGAC AGTGAATCTA AAATAGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCTCC ACAATCTGTC ACTGTGCTGA CAGTTGGAAG CCACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ATCAGAATGG AATCATCCAA
    GAATACAAGA TCTGGTGTCT GGGGAATGAA ACACGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GATCTGTGAT AATTGGTGGA TTGTTCCCAG GTATTCAGTA CCGGGTAGAG
    GTTGCAGCTA GTACAAGTGC AGGGGTTGGA GTAAAAAGTG AGCCGCAGCC GATAATAATT
    GGGGGACGTA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTTGTGA AGCAACCAGC CTTTATAGCT GGAATTGGTG GTGCCTGCTG GGTAATTTTG
    ATGGGTTTTA GCATCTGGTT GTATTGGCGA AGAAAGAAGA GAAAGGGACT CAGTAACTAT
    GCTGTTACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCTGGTCTT
    CTAAATGCTG GTGATCCCAG TTACCCATGG CTTGCTGATT CATGGCCAGC CACGAGCTTA
    CCAGTAAACA ATAGCAATAG TGGCCCAAAT GAGATTGGGA ATTTTGGACG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC GACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACAAC ACAGATCTTG CATTCCAATA GCATACATGA ATTGGCTGTC
    GATCTGCCGG ACCCACAGTG GAAGAGCTCA ATTCAGCAAA AAACGGACCT GATGGGCTTT
    GGATATTCTC TCCCTGATCA GAACAAGGGT AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAGAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCGAC TTGGGCCAAT
    GTACCCCTAC CTCCTCCCCC AGTCCAGCCC CTTCCTGGTA CAGAGCTGGA ACACTATGCA
    GTGGAACCGC AAGAAAATGG GTATGACAGT GATAGCTGGT GCCCGCCATT ACCGGTACAA
    ACATACTTAC ACCAGGGTAT GGAAGATGAA CTGGAAGAAG ATGATGATCG TGTCCCAACA
    CCTCCTGTCC GAGGCGTGGC TTCTTCTCCT GCTATTTCCT TCGGACAGCA GTCCACTGCA
    ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAAGCTCA CCTGGATGAG
    TTGACAAGAG CCTATCAGTT TGATATAGCG AAGCAAACAT GGCACATTCA AAGCAATAAT
    CAACCTCCAC AGCCCCCGGT TCCACCATTA GGTTACATGT CCGGAGCCTT GATTTCCGAT
    TTGGAAACAG ATGTTCCAGA TGATGACGCT GATGATGAGG AGGAAGCTTT AGAAATCCCC
    AGACCCCTGA GAGCACTAGA GCAGACACCC GGATCCAGTA TGGACAATCT AGACAGCTCT
    GTGACAGGCA AAGCTTTTAC CTCCTCTCAA AGACCTCGAC CAACCAGCCC ATTTTCTACT
    GACAGTAATA CCAGTGCAGC CCTGAGTCAA AATCAGAGGC CTCGGCCCAC TAAAAAACAC
    AAGGGAGGGC GAATGGATCA ACAACCAGCA TTGCCTCATC GAAGGGAAGG AATGACAGAT
    GAGGAAACAT TGGTGCCATA CAGCAAGCCC AGTTTCCCAT CTCCGGGAGG TCACAGCTCA
    TCAGGAACAG CATCTTCTAA AGGATCCACT GGACCTAGGA AAGCCGAGGC GTTGAGGGGA
    AGTCACCAAC GCAATGCCAG TGACCTTCTT GACATAGGAT ATATGGGCTC AAATAGTCAA
    GGACAGTTTA CAGGTGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965211.1
    CDS585..4928
    Translation

    Target ORF information:

    RefSeq Version XM_014109736.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109736.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTTACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAAAAAGA AGGCAGCCAG
    AACTTGCTTT TTCCAAATCA ACCCCAGCAA CCCAACAGTA GATGCTCTGT TTCGCCAACT
    GGAGACCTCA CAATTACCAA CATTCAGCGC TCCGATGCGG GTTACTATAT CTGCCAGGCA
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AAGTTACTGA TGTTTTAACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCTGCAAACC AAACTCTAGC AGTTGATGGT
    ACAGCATTAC TGAAATGTAA AGCTACTGGG GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCTGGG TAGAGATTCA AGAGCAACTA TACAAGAACA AGGAACATTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGTACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGGGAGA CTTCGTGGAG TGCTGTGCTG GATGTGACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ACTTAAGTGA CCTACCAGGG CCACCATCCA AACCACAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAA CCAGGTACCC CTGGAGCCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC GGTGGCAAAC
    CATGTAAAGA CCACGCTCTA TACAGTGAGA GGATTGCGGC CCAATACGAT CTATTTATTC
    ATGGTCAGAG CTATCAACCC CCAAGGTCTA AGCGACCCAA GCCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ATAGACAAGT ACAGAAAGAA
    CTAGGAGATG TCATTGTCCG TCTTCATACT CCAGTTGTGT TGACTCCCAC CACTGTTCAG
    GTCACATGGA CGGTTGATCG CCAGCCCCAG TTTATTCAAG GCTACCGAGT GATGTATCGT
    CAGACCACAG GCCTGCAGGC TACGACAACA TGGCAGAATT TAGATGCCAA AGTCCCAACC
    GAGCGAAGTG CTGTCTTGGT CAACCTGAAA AAGGGAGTGA CTTATGAAAT TAAAGTTCGA
    CCATATTTTA ATGAGTTCCA AGGAATGGAC AGTGAATCTA AAATAGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCTCC ACAATCTGTC ACTGTGCTGA CAGTTGGAAG CCACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ATCAGAATGG AATCATCCAA
    GAATACAAGA TCTGGTGTCT GGGGAATGAA ACACGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GATCTGTGAT AATTGGTGGA TTGTTCCCAG GTATTCAGTA CCGGGTAGAG
    GTTGCAGCTA GTACAAGTGC AGGGGTTGGA GTAAAAAGTG AGCCGCAGCC GATAATAATT
    GGGGGACGTA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTTGTGA AGCAACCAGC CTTTATAGCT GGAATTGGTG GTGCCTGCTG GGTAATTTTG
    ATGGGTTTTA GCATCTGGTT GTATTGGCGA AGAAAGAAGA GAAAGGGACT CAGTAACTAT
    GCTGTTACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCTGGTCTT
    CTAAATGCTG GTGATCCCAG TTACCCATGG CTTGCTGATT CATGGCCAGC CACGAGCTTA
    CCAGTAAACA ATAGCAATAG TGGCCCAAAT GAGATTGGGA ATTTTGGACG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC GACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACAAC ACAGATCTTG CATTCCAATA GCATACATGA ATTGGCTGTC
    GATCTGCCGG ACCCACAGTG GAAGAGCTCA ATTCAGCAAA AAACGGACCT GATGGGCTTT
    GGATATTCTC TCCCTGATCA GAACAAGGGT AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAGAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCGAC TTGGGCCAAT
    GTACCCCTAC CTCCTCCCCC AGTCCAGCCC CTTCCTGGTA CAGAGCTGGA ACACTATGCA
    GTGGAACCGC AAGAAAATGG GTATGACAGT GATAGCTGGT GCCCGCCATT ACCGGTACAA
    ACATACTTAC ACCAGGGTAT GGAAGATGAA CTGGAAGAAG ATGATGATCG TGTCCCAACA
    CCTCCTGTCC GAGGCGTGGC TTCTTCTCCT GCTATTTCCT TCGGACAGCA GTCCACTGCA
    ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAAGCTCA CCTGGATGAG
    TTGACAAGAG CCTATCAGTT TGATATAGCG AAGCAAACAT GGCACATTCA AAGCAATAAT
    CAACCTCCAC AGCCCCCGGT TCCACCATTA GGTTACATGT CCGGAGCCTT GATTTCCGAT
    TTGGAAACAG ATGTTCCAGA TGATGACGCT GATGATGAGG AGGAAGCTTT AGAAATCCCC
    AGACCCCTGA GAGCACTAGA GCAGACACCC GGATCCAGTA TGGACAATCT AGACAGCTCT
    GTGACAGGCA AAGCTTTTAC CTCCTCTCAA AGACCTCGAC CAACCAGCCC ATTTTCTACT
    GACAGTAATA CCAGTGCAGC CCTGAGTCAA AATCAGAGGC CTCGGCCCAC TAAAAAACAC
    AAGGGAGGGC GAATGGATCA ACAACCAGCA TTGCCTCATC GAAGGGAAGG AATGACAGAT
    GAGGAAACAT TGGTGCCATA CAGCAAGCCC AGTTTCCCAT CTCCGGGAGG TCACAGCTCA
    TCAGGAACAG CATCTTCTAA AGGATCCACT GGACCTAGGA AAGCCGAGGC GTTGAGGGGA
    AGTCACCAAC GCAATGCCAG TGACCTTCTT GACATAGGAT ATATGGGCTC AAATAGTCAA
    GGACAGTTTA CAGGTGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44455
    Clone ID Related Accession (Same CDS sequence) XM_014109737.1 , XM_014109737.2
    Accession Version XM_014109737.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4218bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product roundabout homolog 2 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTTACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAAAAAGA AGGCAGCCAG
    AACTTGCTTT TTCCAAATCA ACCCCAGCAA CCCAACAGTA GATGCTCTGT TTCGCCAACT
    GGAGACCTCA CAATTACCAA CATTCAGCGC TCCGATGCGG GTTACTATAT CTGCCAGGCA
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AAGTTACTGA TGTTTTAACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCTGCAAACC AAACTCTAGC AGTTGATGGT
    ACAGCATTAC TGAAATGTAA AGCTACTGGG GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCTGGG TAGAGATTCA AGAGCAACTA TACAAGAACA AGGAACATTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGTACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGGGAGA CTTCGTGGAG TGCTGTGCTG GATGTGACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ACTTAAGTGA CCTACCAGGG CCACCATCCA AACCACAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAA CCAGGTACCC CTGGAGCCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC GGTGGCAAAC
    CATGTAAAGA CCACGCTCTA TACAGTGAGA GGATTGCGGC CCAATACGAT CTATTTATTC
    ATGGTCAGAG CTATCAACCC CCAAGGTCTA AGCGACCCAA GCCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ATAGACAAGT ACAGAAAGAA
    CTAGGAGATG TCATTGTCCG TCTTCATACT CCAGTTGTGT TGACTCCCAC CACTGTTCAG
    GTCACATGGA CGGTTGATCG CCAGCCCCAG TTTATTCAAG GCTACCGAGT GATGTATCGT
    CAGACCACAG GCCTGCAGGC TACGACAACA TGGCAGAATT TAGATGCCAA AGTCCCAACC
    GAGCGAAGTG CTGTCTTGGT CAACCTGAAA AAGGGAGTGA CTTATGAAAT TAAAGTTCGA
    CCATATTTTA ATGAGTTCCA AGGAATGGAC AGTGAATCTA AAATAGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCTCC ACAATCTGTC ACTGTGCTGA CAGTTGGAAG CCACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ATCAGAATGG AATCATCCAA
    GAATACAAGA TCTGGTGTCT GGGGAATGAA ACACGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GATCTGTGAT AATTGGTGGA TTGTTCCCAG GTATTCAGTA CCGGGTAGAG
    GTTGCAGCTA GTACAAGTGC AGGGGTTGGA GTAAAAAGTG AGCCGCAGCC GATAATAATT
    GGGGGACGTA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTTGTGA AGCAACCAGC CTTTATAGCT GGAATTGGTG GTGCCTGCTG GGTAATTTTG
    ATGGGTTTTA GCATCTGGTT GTATTGGCGA AGAAAGAAGA GAAAGGGACT CAGTAACTAT
    GCTGTTACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCTGGTCTT
    CTAAATGCTG GTGATCCCAG TTACCCATGG CTTGCTGATT CATGGCCAGC CACGAGCTTA
    CCAGTAAACA ATAGCAATAG TGGCCCAAAT GAGATTGGGA ATTTTGGACG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC GACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACAAC ACAGATCTTG CATTCCAATA GCATACATGA ATTGGCTGTC
    GATCTGCCGG ACCCACAGTG GAAGAGCTCA ATTCAGCAAA AAACGGACCT GATGGGCTTT
    GGATATTCTC TCCCTGATCA GAACAAGGGT AACAACGGTG GGAAAGGTGG AAAAAAGAAG
    AAAACTAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CGACTTGGGC CAATGTACCC
    CTACCTCCTC CCCCAGTCCA GCCCCTTCCT GGTACAGAGC TGGAACACTA TGCAGTGGAA
    CCGCAAGAAA ATGGGTATGA CAGTGATAGC TGGTGCCCGC CATTACCGGT ACAAACATAC
    TTACACCAGG GTATGGAAGA TGAACTGGAA GAAGATGATG ATCGTGTCCC AACACCTCCT
    GTCCGAGGCG TGGCTTCTTC TCCTGCTATT TCCTTCGGAC AGCAGTCCAC TGCAACTCTT
    ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAAG CTCACCTGGA TGAGTTGACA
    AGAGCCTATC AGTTTGATAT AGCGAAGCAA ACATGGCACA TTCAAAGCAA TAATCAACCT
    CCACAGCCCC CGGTTCCACC ATTAGGTTAC ATGTCCGGAG CCTTGATTTC CGATTTGGAA
    ACAGATGTTC CAGATGATGA CGCTGATGAT GAGGAGGAAG CTTTAGAAAT CCCCAGACCC
    CTGAGAGCAC TAGAGCAGAC ACCCGGATCC AGTATGGACA ATCTAGACAG CTCTGTGACA
    GGCAAAGCTT TTACCTCCTC TCAAAGACCT CGACCAACCA GCCCATTTTC TACTGACAGT
    AATACCAGTG CAGCCCTGAG TCAAAATCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA
    GGGCGAATGG ATCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGAGGAA
    ACATTGGTGC CATACAGCAA GCCCAGTTTC CCATCTCCGG GAGGTCACAG CTCATCAGGA
    ACAGCATCTT CTAAAGGATC CACTGGACCT AGGAAAGCCG AGGCGTTGAG GGGAAGTCAC
    CAACGCAATG CCAGTGACCT TCTTGACATA GGATATATGG GCTCAAATAG TCAAGGACAG
    TTTACAGGTG AATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965212.1
    CDS584..4801
    Translation

    Target ORF information:

    RefSeq Version XM_014109737.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109737.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTTACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAAAAAGA AGGCAGCCAG
    AACTTGCTTT TTCCAAATCA ACCCCAGCAA CCCAACAGTA GATGCTCTGT TTCGCCAACT
    GGAGACCTCA CAATTACCAA CATTCAGCGC TCCGATGCGG GTTACTATAT CTGCCAGGCA
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AAGTTACTGA TGTTTTAACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCTGCAAACC AAACTCTAGC AGTTGATGGT
    ACAGCATTAC TGAAATGTAA AGCTACTGGG GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCTGGG TAGAGATTCA AGAGCAACTA TACAAGAACA AGGAACATTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGTACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGGGAGA CTTCGTGGAG TGCTGTGCTG GATGTGACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ACTTAAGTGA CCTACCAGGG CCACCATCCA AACCACAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAA CCAGGTACCC CTGGAGCCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC GGTGGCAAAC
    CATGTAAAGA CCACGCTCTA TACAGTGAGA GGATTGCGGC CCAATACGAT CTATTTATTC
    ATGGTCAGAG CTATCAACCC CCAAGGTCTA AGCGACCCAA GCCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ATAGACAAGT ACAGAAAGAA
    CTAGGAGATG TCATTGTCCG TCTTCATACT CCAGTTGTGT TGACTCCCAC CACTGTTCAG
    GTCACATGGA CGGTTGATCG CCAGCCCCAG TTTATTCAAG GCTACCGAGT GATGTATCGT
    CAGACCACAG GCCTGCAGGC TACGACAACA TGGCAGAATT TAGATGCCAA AGTCCCAACC
    GAGCGAAGTG CTGTCTTGGT CAACCTGAAA AAGGGAGTGA CTTATGAAAT TAAAGTTCGA
    CCATATTTTA ATGAGTTCCA AGGAATGGAC AGTGAATCTA AAATAGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCTCC ACAATCTGTC ACTGTGCTGA CAGTTGGAAG CCACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ATCAGAATGG AATCATCCAA
    GAATACAAGA TCTGGTGTCT GGGGAATGAA ACACGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GATCTGTGAT AATTGGTGGA TTGTTCCCAG GTATTCAGTA CCGGGTAGAG
    GTTGCAGCTA GTACAAGTGC AGGGGTTGGA GTAAAAAGTG AGCCGCAGCC GATAATAATT
    GGGGGACGTA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTTGTGA AGCAACCAGC CTTTATAGCT GGAATTGGTG GTGCCTGCTG GGTAATTTTG
    ATGGGTTTTA GCATCTGGTT GTATTGGCGA AGAAAGAAGA GAAAGGGACT CAGTAACTAT
    GCTGTTACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCTGGTCTT
    CTAAATGCTG GTGATCCCAG TTACCCATGG CTTGCTGATT CATGGCCAGC CACGAGCTTA
    CCAGTAAACA ATAGCAATAG TGGCCCAAAT GAGATTGGGA ATTTTGGACG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC GACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACAAC ACAGATCTTG CATTCCAATA GCATACATGA ATTGGCTGTC
    GATCTGCCGG ACCCACAGTG GAAGAGCTCA ATTCAGCAAA AAACGGACCT GATGGGCTTT
    GGATATTCTC TCCCTGATCA GAACAAGGGT AACAACGGTG GGAAAGGTGG AAAAAAGAAG
    AAAACTAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CGACTTGGGC CAATGTACCC
    CTACCTCCTC CCCCAGTCCA GCCCCTTCCT GGTACAGAGC TGGAACACTA TGCAGTGGAA
    CCGCAAGAAA ATGGGTATGA CAGTGATAGC TGGTGCCCGC CATTACCGGT ACAAACATAC
    TTACACCAGG GTATGGAAGA TGAACTGGAA GAAGATGATG ATCGTGTCCC AACACCTCCT
    GTCCGAGGCG TGGCTTCTTC TCCTGCTATT TCCTTCGGAC AGCAGTCCAC TGCAACTCTT
    ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAAG CTCACCTGGA TGAGTTGACA
    AGAGCCTATC AGTTTGATAT AGCGAAGCAA ACATGGCACA TTCAAAGCAA TAATCAACCT
    CCACAGCCCC CGGTTCCACC ATTAGGTTAC ATGTCCGGAG CCTTGATTTC CGATTTGGAA
    ACAGATGTTC CAGATGATGA CGCTGATGAT GAGGAGGAAG CTTTAGAAAT CCCCAGACCC
    CTGAGAGCAC TAGAGCAGAC ACCCGGATCC AGTATGGACA ATCTAGACAG CTCTGTGACA
    GGCAAAGCTT TTACCTCCTC TCAAAGACCT CGACCAACCA GCCCATTTTC TACTGACAGT
    AATACCAGTG CAGCCCTGAG TCAAAATCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA
    GGGCGAATGG ATCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGAGGAA
    ACATTGGTGC CATACAGCAA GCCCAGTTTC CCATCTCCGG GAGGTCACAG CTCATCAGGA
    ACAGCATCTT CTAAAGGATC CACTGGACCT AGGAAAGCCG AGGCGTTGAG GGGAAGTCAC
    CAACGCAATG CCAGTGACCT TCTTGACATA GGATATATGG GCTCAAATAG TCAAGGACAG
    TTTACAGGTG AATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44455
    Clone ID Related Accession (Same CDS sequence) XM_014109737.1 , XM_014109737.2
    Accession Version XM_014109737.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4218bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product roundabout homolog 2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109737.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTTACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAAAAAGA AGGCAGCCAG
    AACTTGCTTT TTCCAAATCA ACCCCAGCAA CCCAACAGTA GATGCTCTGT TTCGCCAACT
    GGAGACCTCA CAATTACCAA CATTCAGCGC TCCGATGCGG GTTACTATAT CTGCCAGGCA
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AAGTTACTGA TGTTTTAACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCTGCAAACC AAACTCTAGC AGTTGATGGT
    ACAGCATTAC TGAAATGTAA AGCTACTGGG GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCTGGG TAGAGATTCA AGAGCAACTA TACAAGAACA AGGAACATTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGTACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGGGAGA CTTCGTGGAG TGCTGTGCTG GATGTGACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ACTTAAGTGA CCTACCAGGG CCACCATCCA AACCACAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAA CCAGGTACCC CTGGAGCCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC GGTGGCAAAC
    CATGTAAAGA CCACGCTCTA TACAGTGAGA GGATTGCGGC CCAATACGAT CTATTTATTC
    ATGGTCAGAG CTATCAACCC CCAAGGTCTA AGCGACCCAA GCCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ATAGACAAGT ACAGAAAGAA
    CTAGGAGATG TCATTGTCCG TCTTCATACT CCAGTTGTGT TGACTCCCAC CACTGTTCAG
    GTCACATGGA CGGTTGATCG CCAGCCCCAG TTTATTCAAG GCTACCGAGT GATGTATCGT
    CAGACCACAG GCCTGCAGGC TACGACAACA TGGCAGAATT TAGATGCCAA AGTCCCAACC
    GAGCGAAGTG CTGTCTTGGT CAACCTGAAA AAGGGAGTGA CTTATGAAAT TAAAGTTCGA
    CCATATTTTA ATGAGTTCCA AGGAATGGAC AGTGAATCTA AAATAGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCTCC ACAATCTGTC ACTGTGCTGA CAGTTGGAAG CCACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ATCAGAATGG AATCATCCAA
    GAATACAAGA TCTGGTGTCT GGGGAATGAA ACACGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GATCTGTGAT AATTGGTGGA TTGTTCCCAG GTATTCAGTA CCGGGTAGAG
    GTTGCAGCTA GTACAAGTGC AGGGGTTGGA GTAAAAAGTG AGCCGCAGCC GATAATAATT
    GGGGGACGTA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTTGTGA AGCAACCAGC CTTTATAGCT GGAATTGGTG GTGCCTGCTG GGTAATTTTG
    ATGGGTTTTA GCATCTGGTT GTATTGGCGA AGAAAGAAGA GAAAGGGACT CAGTAACTAT
    GCTGTTACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCTGGTCTT
    CTAAATGCTG GTGATCCCAG TTACCCATGG CTTGCTGATT CATGGCCAGC CACGAGCTTA
    CCAGTAAACA ATAGCAATAG TGGCCCAAAT GAGATTGGGA ATTTTGGACG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC GACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACAAC ACAGATCTTG CATTCCAATA GCATACATGA ATTGGCTGTC
    GATCTGCCGG ACCCACAGTG GAAGAGCTCA ATTCAGCAAA AAACGGACCT GATGGGCTTT
    GGATATTCTC TCCCTGATCA GAACAAGGGT AACAACGGTG GGAAAGGTGG AAAAAAGAAG
    AAAACTAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CGACTTGGGC CAATGTACCC
    CTACCTCCTC CCCCAGTCCA GCCCCTTCCT GGTACAGAGC TGGAACACTA TGCAGTGGAA
    CCGCAAGAAA ATGGGTATGA CAGTGATAGC TGGTGCCCGC CATTACCGGT ACAAACATAC
    TTACACCAGG GTATGGAAGA TGAACTGGAA GAAGATGATG ATCGTGTCCC AACACCTCCT
    GTCCGAGGCG TGGCTTCTTC TCCTGCTATT TCCTTCGGAC AGCAGTCCAC TGCAACTCTT
    ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAAG CTCACCTGGA TGAGTTGACA
    AGAGCCTATC AGTTTGATAT AGCGAAGCAA ACATGGCACA TTCAAAGCAA TAATCAACCT
    CCACAGCCCC CGGTTCCACC ATTAGGTTAC ATGTCCGGAG CCTTGATTTC CGATTTGGAA
    ACAGATGTTC CAGATGATGA CGCTGATGAT GAGGAGGAAG CTTTAGAAAT CCCCAGACCC
    CTGAGAGCAC TAGAGCAGAC ACCCGGATCC AGTATGGACA ATCTAGACAG CTCTGTGACA
    GGCAAAGCTT TTACCTCCTC TCAAAGACCT CGACCAACCA GCCCATTTTC TACTGACAGT
    AATACCAGTG CAGCCCTGAG TCAAAATCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA
    GGGCGAATGG ATCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGAGGAA
    ACATTGGTGC CATACAGCAA GCCCAGTTTC CCATCTCCGG GAGGTCACAG CTCATCAGGA
    ACAGCATCTT CTAAAGGATC CACTGGACCT AGGAAAGCCG AGGCGTTGAG GGGAAGTCAC
    CAACGCAATG CCAGTGACCT TCTTGACATA GGATATATGG GCTCAAATAG TCAAGGACAG
    TTTACAGGTG AATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965212.1
    CDS384..4601
    Translation

    Target ORF information:

    RefSeq Version XM_014109737.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109737.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTTACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAAAAAGA AGGCAGCCAG
    AACTTGCTTT TTCCAAATCA ACCCCAGCAA CCCAACAGTA GATGCTCTGT TTCGCCAACT
    GGAGACCTCA CAATTACCAA CATTCAGCGC TCCGATGCGG GTTACTATAT CTGCCAGGCA
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AAGTTACTGA TGTTTTAACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCTGCAAACC AAACTCTAGC AGTTGATGGT
    ACAGCATTAC TGAAATGTAA AGCTACTGGG GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCTGGG TAGAGATTCA AGAGCAACTA TACAAGAACA AGGAACATTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGTACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGGGAGA CTTCGTGGAG TGCTGTGCTG GATGTGACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ACTTAAGTGA CCTACCAGGG CCACCATCCA AACCACAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAA CCAGGTACCC CTGGAGCCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC GGTGGCAAAC
    CATGTAAAGA CCACGCTCTA TACAGTGAGA GGATTGCGGC CCAATACGAT CTATTTATTC
    ATGGTCAGAG CTATCAACCC CCAAGGTCTA AGCGACCCAA GCCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ATAGACAAGT ACAGAAAGAA
    CTAGGAGATG TCATTGTCCG TCTTCATACT CCAGTTGTGT TGACTCCCAC CACTGTTCAG
    GTCACATGGA CGGTTGATCG CCAGCCCCAG TTTATTCAAG GCTACCGAGT GATGTATCGT
    CAGACCACAG GCCTGCAGGC TACGACAACA TGGCAGAATT TAGATGCCAA AGTCCCAACC
    GAGCGAAGTG CTGTCTTGGT CAACCTGAAA AAGGGAGTGA CTTATGAAAT TAAAGTTCGA
    CCATATTTTA ATGAGTTCCA AGGAATGGAC AGTGAATCTA AAATAGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCTCC ACAATCTGTC ACTGTGCTGA CAGTTGGAAG CCACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ATCAGAATGG AATCATCCAA
    GAATACAAGA TCTGGTGTCT GGGGAATGAA ACACGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GATCTGTGAT AATTGGTGGA TTGTTCCCAG GTATTCAGTA CCGGGTAGAG
    GTTGCAGCTA GTACAAGTGC AGGGGTTGGA GTAAAAAGTG AGCCGCAGCC GATAATAATT
    GGGGGACGTA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTTGTGA AGCAACCAGC CTTTATAGCT GGAATTGGTG GTGCCTGCTG GGTAATTTTG
    ATGGGTTTTA GCATCTGGTT GTATTGGCGA AGAAAGAAGA GAAAGGGACT CAGTAACTAT
    GCTGTTACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCTGGTCTT
    CTAAATGCTG GTGATCCCAG TTACCCATGG CTTGCTGATT CATGGCCAGC CACGAGCTTA
    CCAGTAAACA ATAGCAATAG TGGCCCAAAT GAGATTGGGA ATTTTGGACG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC GACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACAAC ACAGATCTTG CATTCCAATA GCATACATGA ATTGGCTGTC
    GATCTGCCGG ACCCACAGTG GAAGAGCTCA ATTCAGCAAA AAACGGACCT GATGGGCTTT
    GGATATTCTC TCCCTGATCA GAACAAGGGT AACAACGGTG GGAAAGGTGG AAAAAAGAAG
    AAAACTAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CGACTTGGGC CAATGTACCC
    CTACCTCCTC CCCCAGTCCA GCCCCTTCCT GGTACAGAGC TGGAACACTA TGCAGTGGAA
    CCGCAAGAAA ATGGGTATGA CAGTGATAGC TGGTGCCCGC CATTACCGGT ACAAACATAC
    TTACACCAGG GTATGGAAGA TGAACTGGAA GAAGATGATG ATCGTGTCCC AACACCTCCT
    GTCCGAGGCG TGGCTTCTTC TCCTGCTATT TCCTTCGGAC AGCAGTCCAC TGCAACTCTT
    ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAAG CTCACCTGGA TGAGTTGACA
    AGAGCCTATC AGTTTGATAT AGCGAAGCAA ACATGGCACA TTCAAAGCAA TAATCAACCT
    CCACAGCCCC CGGTTCCACC ATTAGGTTAC ATGTCCGGAG CCTTGATTTC CGATTTGGAA
    ACAGATGTTC CAGATGATGA CGCTGATGAT GAGGAGGAAG CTTTAGAAAT CCCCAGACCC
    CTGAGAGCAC TAGAGCAGAC ACCCGGATCC AGTATGGACA ATCTAGACAG CTCTGTGACA
    GGCAAAGCTT TTACCTCCTC TCAAAGACCT CGACCAACCA GCCCATTTTC TACTGACAGT
    AATACCAGTG CAGCCCTGAG TCAAAATCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA
    GGGCGAATGG ATCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGAGGAA
    ACATTGGTGC CATACAGCAA GCCCAGTTTC CCATCTCCGG GAGGTCACAG CTCATCAGGA
    ACAGCATCTT CTAAAGGATC CACTGGACCT AGGAAAGCCG AGGCGTTGAG GGGAAGTCAC
    CAACGCAATG CCAGTGACCT TCTTGACATA GGATATATGG GCTCAAATAG TCAAGGACAG
    TTTACAGGTG AATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44456
    Clone ID Related Accession (Same CDS sequence) XM_014109738.2 , XM_014109738.1
    Accession Version XM_014109738.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product roundabout homolog 2 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109738.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCCTCC ACAGTTTGTG
    GTTAGGCCAA GAGATCAGAT TGTTGCTCAA GGTCGAACAG TTACATTTCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAAAAAGAAG GCAGCCAGAA CTTGCTTTTT
    CCAAATCAAC CCCAGCAACC CAACAGTAGA TGCTCTGTTT CGCCAACTGG AGACCTCACA
    ATTACCAACA TTCAGCGCTC CGATGCGGGT TACTATATCT GCCAGGCATT AACTGTGGCA
    GGAAGCATTT TAGCAAAAGC TCAACTGGAA GTTACTGATG TTTTAACAGA TAGACCTCCA
    CCTATAATTC TACAAGGCCC TGCAAACCAA ACTCTAGCAG TTGATGGTAC AGCATTACTG
    AAATGTAAAG CTACTGGGGA TCCTCTTCCT GTAATTAGCT GGTTAAAGGA GGGATTTACT
    TTTCTGGGTA GAGATTCAAG AGCAACTATA CAAGAACAAG GAACATTGCA GATTAAGAAT
    TTACGGATTT CTGATACTGG TACTTATACT TGTGTGGCTA CAAGTTCAAG TGGGGAGACT
    TCGTGGAGTG CTGTGCTGGA TGTGACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAC
    TTAAGTGACC TACCAGGGCC ACCATCCAAA CCACAGGTCA CTGATGTTAC TAAGAACAGT
    GTCACCTTGT CCTGGCAACC AGGTACCCCT GGAGCCCTTC CAGCAAGTGC ATATATCATT
    GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACGG TGGCAAACCA TGTAAAGACC
    ACGCTCTATA CAGTGAGAGG ATTGCGGCCC AATACGATCT ATTTATTCAT GGTCAGAGCT
    ATCAACCCCC AAGGTCTAAG CGACCCAAGC CCCATGTCAG ATCCTGTGCG CACACAAGAT
    ATCAGCCCAC CAGCACAAGG AGTGGACCAT AGACAAGTAC AGAAAGAACT AGGAGATGTC
    ATTGTCCGTC TTCATACTCC AGTTGTGTTG ACTCCCACCA CTGTTCAGGT CACATGGACG
    GTTGATCGCC AGCCCCAGTT TATTCAAGGC TACCGAGTGA TGTATCGTCA GACCACAGGC
    CTGCAGGCTA CGACAACATG GCAGAATTTA GATGCCAAAG TCCCAACCGA GCGAAGTGCT
    GTCTTGGTCA ACCTGAAAAA GGGAGTGACT TATGAAATTA AAGTTCGACC ATATTTTAAT
    GAGTTCCAAG GAATGGACAG TGAATCTAAA ATAGTTCGTA CTACTGAAGA AGCCCCAAGT
    GCCCCTCCAC AATCTGTCAC TGTGCTGACA GTTGGAAGCC ACAATAGCAC AAGTATTAGT
    GTTTCCTGGG ATCCTCCTCC TCCAGATCAT CAGAATGGAA TCATCCAAGA ATACAAGATC
    TGGTGTCTGG GGAATGAAAC ACGATTCCAT ATCAACAAAA CTGTGGATGC AGCCATTCGA
    TCTGTGATAA TTGGTGGATT GTTCCCAGGT ATTCAGTACC GGGTAGAGGT TGCAGCTAGT
    ACAAGTGCAG GGGTTGGAGT AAAAAGTGAG CCGCAGCCGA TAATAATTGG GGGACGTAAT
    GAAGTTGTCA TTACTGAAAA CAATAACAGC ATAACTGAGC AAATCACTGA TGTTGTGAAG
    CAACCAGCCT TTATAGCTGG AATTGGTGGT GCCTGCTGGG TAATTTTGAT GGGTTTTAGC
    ATCTGGTTGT ATTGGCGAAG AAAGAAGAGA AAGGGACTCA GTAACTATGC TGTTACATTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CTGGTCTTCT AAATGCTGGT
    GATCCCAGTT ACCCATGGCT TGCTGATTCA TGGCCAGCCA CGAGCTTACC AGTAAACAAT
    AGCAATAGTG GCCCAAATGA GATTGGGAAT TTTGGACGTG GAGATGTGCT GCCACCAGTT
    CCAGGCCAAG GGGATAAAAC AGCAACGATG CTCTCAGATG GAGCCATTTA TAGCAGCATT
    GACTTCACGA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC TACCCCATAT
    GCCACAACAC AGATCTTGCA TTCCAATAGC ATACATGAAT TGGCTGTCGA TCTGCCGGAC
    CCACAGTGGA AGAGCTCAAT TCAGCAAAAA ACGGACCTGA TGGGCTTTGG ATATTCTCTC
    CCTGATCAGA ACAAGGGTAA CAACGGTGGG AAAGGTGGAA AAAAGAAGAA AACTAAAAAC
    TCTTCTAAAC CACAGAAAAA CAATGGATCG ACTTGGGCCA ATGTACCCCT ACCTCCTCCC
    CCAGTCCAGC CCCTTCCTGG TACAGAGCTG GAACACTATG CAGTGGAACC GCAAGAAAAT
    GGGTATGACA GTGATAGCTG GTGCCCGCCA TTACCGGTAC AAACATACTT ACACCAGGGT
    ATGGAAGATG AACTGGAAGA AGATGATGAT CGTGTCCCAA CACCTCCTGT CCGAGGCGTG
    GCTTCTTCTC CTGCTATTTC CTTCGGACAG CAGTCCACTG CAACTCTTAC TCCATCCCCA
    CGGGAAGAGA TGCAACCCAT GCTGCAAGCT CACCTGGATG AGTTGACAAG AGCCTATCAG
    TTTGATATAG CGAAGCAAAC ATGGCACATT CAAAGCAATA ATCAACCTCC ACAGCCCCCG
    GTTCCACCAT TAGGTTACAT GTCCGGAGCC TTGATTTCCG ATTTGGAAAC AGATGTTCCA
    GATGATGACG CTGATGATGA GGAGGAAGCT TTAGAAATCC CCAGACCCCT GAGAGCACTA
    GAGCAGACAC CCGGATCCAG TATGGACAAT CTAGACAGCT CTGTGACAGG CAAAGCTTTT
    ACCTCCTCTC AAAGACCTCG ACCAACCAGC CCATTTTCTA CTGACAGTAA TACCAGTGCA
    GCCCTGAGTC AAAATCAGAG GCCTCGGCCC ACTAAAAAAC ACAAGGGAGG GCGAATGGAT
    CAACAACCAG CATTGCCTCA TCGAAGGGAA GGAATGACAG ATGAGGAAAC ATTGGTGCCA
    TACAGCAAGC CCAGTTTCCC ATCTCCGGGA GGTCACAGCT CATCAGGAAC AGCATCTTCT
    AAAGGATCCA CTGGACCTAG GAAAGCCGAG GCGTTGAGGG GAAGTCACCA ACGCAATGCC
    AGTGACCTTC TTGACATAGG ATATATGGGC TCAAATAGTC AAGGACAGTT TACAGGTGAA
    TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965213.1
    CDS384..4589
    Translation

    Target ORF information:

    RefSeq Version XM_014109738.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109738.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCCTCC ACAGTTTGTG
    GTTAGGCCAA GAGATCAGAT TGTTGCTCAA GGTCGAACAG TTACATTTCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAAAAAGAAG GCAGCCAGAA CTTGCTTTTT
    CCAAATCAAC CCCAGCAACC CAACAGTAGA TGCTCTGTTT CGCCAACTGG AGACCTCACA
    ATTACCAACA TTCAGCGCTC CGATGCGGGT TACTATATCT GCCAGGCATT AACTGTGGCA
    GGAAGCATTT TAGCAAAAGC TCAACTGGAA GTTACTGATG TTTTAACAGA TAGACCTCCA
    CCTATAATTC TACAAGGCCC TGCAAACCAA ACTCTAGCAG TTGATGGTAC AGCATTACTG
    AAATGTAAAG CTACTGGGGA TCCTCTTCCT GTAATTAGCT GGTTAAAGGA GGGATTTACT
    TTTCTGGGTA GAGATTCAAG AGCAACTATA CAAGAACAAG GAACATTGCA GATTAAGAAT
    TTACGGATTT CTGATACTGG TACTTATACT TGTGTGGCTA CAAGTTCAAG TGGGGAGACT
    TCGTGGAGTG CTGTGCTGGA TGTGACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAC
    TTAAGTGACC TACCAGGGCC ACCATCCAAA CCACAGGTCA CTGATGTTAC TAAGAACAGT
    GTCACCTTGT CCTGGCAACC AGGTACCCCT GGAGCCCTTC CAGCAAGTGC ATATATCATT
    GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACGG TGGCAAACCA TGTAAAGACC
    ACGCTCTATA CAGTGAGAGG ATTGCGGCCC AATACGATCT ATTTATTCAT GGTCAGAGCT
    ATCAACCCCC AAGGTCTAAG CGACCCAAGC CCCATGTCAG ATCCTGTGCG CACACAAGAT
    ATCAGCCCAC CAGCACAAGG AGTGGACCAT AGACAAGTAC AGAAAGAACT AGGAGATGTC
    ATTGTCCGTC TTCATACTCC AGTTGTGTTG ACTCCCACCA CTGTTCAGGT CACATGGACG
    GTTGATCGCC AGCCCCAGTT TATTCAAGGC TACCGAGTGA TGTATCGTCA GACCACAGGC
    CTGCAGGCTA CGACAACATG GCAGAATTTA GATGCCAAAG TCCCAACCGA GCGAAGTGCT
    GTCTTGGTCA ACCTGAAAAA GGGAGTGACT TATGAAATTA AAGTTCGACC ATATTTTAAT
    GAGTTCCAAG GAATGGACAG TGAATCTAAA ATAGTTCGTA CTACTGAAGA AGCCCCAAGT
    GCCCCTCCAC AATCTGTCAC TGTGCTGACA GTTGGAAGCC ACAATAGCAC AAGTATTAGT
    GTTTCCTGGG ATCCTCCTCC TCCAGATCAT CAGAATGGAA TCATCCAAGA ATACAAGATC
    TGGTGTCTGG GGAATGAAAC ACGATTCCAT ATCAACAAAA CTGTGGATGC AGCCATTCGA
    TCTGTGATAA TTGGTGGATT GTTCCCAGGT ATTCAGTACC GGGTAGAGGT TGCAGCTAGT
    ACAAGTGCAG GGGTTGGAGT AAAAAGTGAG CCGCAGCCGA TAATAATTGG GGGACGTAAT
    GAAGTTGTCA TTACTGAAAA CAATAACAGC ATAACTGAGC AAATCACTGA TGTTGTGAAG
    CAACCAGCCT TTATAGCTGG AATTGGTGGT GCCTGCTGGG TAATTTTGAT GGGTTTTAGC
    ATCTGGTTGT ATTGGCGAAG AAAGAAGAGA AAGGGACTCA GTAACTATGC TGTTACATTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CTGGTCTTCT AAATGCTGGT
    GATCCCAGTT ACCCATGGCT TGCTGATTCA TGGCCAGCCA CGAGCTTACC AGTAAACAAT
    AGCAATAGTG GCCCAAATGA GATTGGGAAT TTTGGACGTG GAGATGTGCT GCCACCAGTT
    CCAGGCCAAG GGGATAAAAC AGCAACGATG CTCTCAGATG GAGCCATTTA TAGCAGCATT
    GACTTCACGA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC TACCCCATAT
    GCCACAACAC AGATCTTGCA TTCCAATAGC ATACATGAAT TGGCTGTCGA TCTGCCGGAC
    CCACAGTGGA AGAGCTCAAT TCAGCAAAAA ACGGACCTGA TGGGCTTTGG ATATTCTCTC
    CCTGATCAGA ACAAGGGTAA CAACGGTGGG AAAGGTGGAA AAAAGAAGAA AACTAAAAAC
    TCTTCTAAAC CACAGAAAAA CAATGGATCG ACTTGGGCCA ATGTACCCCT ACCTCCTCCC
    CCAGTCCAGC CCCTTCCTGG TACAGAGCTG GAACACTATG CAGTGGAACC GCAAGAAAAT
    GGGTATGACA GTGATAGCTG GTGCCCGCCA TTACCGGTAC AAACATACTT ACACCAGGGT
    ATGGAAGATG AACTGGAAGA AGATGATGAT CGTGTCCCAA CACCTCCTGT CCGAGGCGTG
    GCTTCTTCTC CTGCTATTTC CTTCGGACAG CAGTCCACTG CAACTCTTAC TCCATCCCCA
    CGGGAAGAGA TGCAACCCAT GCTGCAAGCT CACCTGGATG AGTTGACAAG AGCCTATCAG
    TTTGATATAG CGAAGCAAAC ATGGCACATT CAAAGCAATA ATCAACCTCC ACAGCCCCCG
    GTTCCACCAT TAGGTTACAT GTCCGGAGCC TTGATTTCCG ATTTGGAAAC AGATGTTCCA
    GATGATGACG CTGATGATGA GGAGGAAGCT TTAGAAATCC CCAGACCCCT GAGAGCACTA
    GAGCAGACAC CCGGATCCAG TATGGACAAT CTAGACAGCT CTGTGACAGG CAAAGCTTTT
    ACCTCCTCTC AAAGACCTCG ACCAACCAGC CCATTTTCTA CTGACAGTAA TACCAGTGCA
    GCCCTGAGTC AAAATCAGAG GCCTCGGCCC ACTAAAAAAC ACAAGGGAGG GCGAATGGAT
    CAACAACCAG CATTGCCTCA TCGAAGGGAA GGAATGACAG ATGAGGAAAC ATTGGTGCCA
    TACAGCAAGC CCAGTTTCCC ATCTCCGGGA GGTCACAGCT CATCAGGAAC AGCATCTTCT
    AAAGGATCCA CTGGACCTAG GAAAGCCGAG GCGTTGAGGG GAAGTCACCA ACGCAATGCC
    AGTGACCTTC TTGACATAGG ATATATGGGC TCAAATAGTC AAGGACAGTT TACAGGTGAA
    TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44456
    Clone ID Related Accession (Same CDS sequence) XM_014109738.2 , XM_014109738.1
    Accession Version XM_014109738.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product roundabout homolog 2 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCCTCC ACAGTTTGTG
    GTTAGGCCAA GAGATCAGAT TGTTGCTCAA GGTCGAACAG TTACATTTCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAAAAAGAAG GCAGCCAGAA CTTGCTTTTT
    CCAAATCAAC CCCAGCAACC CAACAGTAGA TGCTCTGTTT CGCCAACTGG AGACCTCACA
    ATTACCAACA TTCAGCGCTC CGATGCGGGT TACTATATCT GCCAGGCATT AACTGTGGCA
    GGAAGCATTT TAGCAAAAGC TCAACTGGAA GTTACTGATG TTTTAACAGA TAGACCTCCA
    CCTATAATTC TACAAGGCCC TGCAAACCAA ACTCTAGCAG TTGATGGTAC AGCATTACTG
    AAATGTAAAG CTACTGGGGA TCCTCTTCCT GTAATTAGCT GGTTAAAGGA GGGATTTACT
    TTTCTGGGTA GAGATTCAAG AGCAACTATA CAAGAACAAG GAACATTGCA GATTAAGAAT
    TTACGGATTT CTGATACTGG TACTTATACT TGTGTGGCTA CAAGTTCAAG TGGGGAGACT
    TCGTGGAGTG CTGTGCTGGA TGTGACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAC
    TTAAGTGACC TACCAGGGCC ACCATCCAAA CCACAGGTCA CTGATGTTAC TAAGAACAGT
    GTCACCTTGT CCTGGCAACC AGGTACCCCT GGAGCCCTTC CAGCAAGTGC ATATATCATT
    GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACGG TGGCAAACCA TGTAAAGACC
    ACGCTCTATA CAGTGAGAGG ATTGCGGCCC AATACGATCT ATTTATTCAT GGTCAGAGCT
    ATCAACCCCC AAGGTCTAAG CGACCCAAGC CCCATGTCAG ATCCTGTGCG CACACAAGAT
    ATCAGCCCAC CAGCACAAGG AGTGGACCAT AGACAAGTAC AGAAAGAACT AGGAGATGTC
    ATTGTCCGTC TTCATACTCC AGTTGTGTTG ACTCCCACCA CTGTTCAGGT CACATGGACG
    GTTGATCGCC AGCCCCAGTT TATTCAAGGC TACCGAGTGA TGTATCGTCA GACCACAGGC
    CTGCAGGCTA CGACAACATG GCAGAATTTA GATGCCAAAG TCCCAACCGA GCGAAGTGCT
    GTCTTGGTCA ACCTGAAAAA GGGAGTGACT TATGAAATTA AAGTTCGACC ATATTTTAAT
    GAGTTCCAAG GAATGGACAG TGAATCTAAA ATAGTTCGTA CTACTGAAGA AGCCCCAAGT
    GCCCCTCCAC AATCTGTCAC TGTGCTGACA GTTGGAAGCC ACAATAGCAC AAGTATTAGT
    GTTTCCTGGG ATCCTCCTCC TCCAGATCAT CAGAATGGAA TCATCCAAGA ATACAAGATC
    TGGTGTCTGG GGAATGAAAC ACGATTCCAT ATCAACAAAA CTGTGGATGC AGCCATTCGA
    TCTGTGATAA TTGGTGGATT GTTCCCAGGT ATTCAGTACC GGGTAGAGGT TGCAGCTAGT
    ACAAGTGCAG GGGTTGGAGT AAAAAGTGAG CCGCAGCCGA TAATAATTGG GGGACGTAAT
    GAAGTTGTCA TTACTGAAAA CAATAACAGC ATAACTGAGC AAATCACTGA TGTTGTGAAG
    CAACCAGCCT TTATAGCTGG AATTGGTGGT GCCTGCTGGG TAATTTTGAT GGGTTTTAGC
    ATCTGGTTGT ATTGGCGAAG AAAGAAGAGA AAGGGACTCA GTAACTATGC TGTTACATTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CTGGTCTTCT AAATGCTGGT
    GATCCCAGTT ACCCATGGCT TGCTGATTCA TGGCCAGCCA CGAGCTTACC AGTAAACAAT
    AGCAATAGTG GCCCAAATGA GATTGGGAAT TTTGGACGTG GAGATGTGCT GCCACCAGTT
    CCAGGCCAAG GGGATAAAAC AGCAACGATG CTCTCAGATG GAGCCATTTA TAGCAGCATT
    GACTTCACGA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC TACCCCATAT
    GCCACAACAC AGATCTTGCA TTCCAATAGC ATACATGAAT TGGCTGTCGA TCTGCCGGAC
    CCACAGTGGA AGAGCTCAAT TCAGCAAAAA ACGGACCTGA TGGGCTTTGG ATATTCTCTC
    CCTGATCAGA ACAAGGGTAA CAACGGTGGG AAAGGTGGAA AAAAGAAGAA AACTAAAAAC
    TCTTCTAAAC CACAGAAAAA CAATGGATCG ACTTGGGCCA ATGTACCCCT ACCTCCTCCC
    CCAGTCCAGC CCCTTCCTGG TACAGAGCTG GAACACTATG CAGTGGAACC GCAAGAAAAT
    GGGTATGACA GTGATAGCTG GTGCCCGCCA TTACCGGTAC AAACATACTT ACACCAGGGT
    ATGGAAGATG AACTGGAAGA AGATGATGAT CGTGTCCCAA CACCTCCTGT CCGAGGCGTG
    GCTTCTTCTC CTGCTATTTC CTTCGGACAG CAGTCCACTG CAACTCTTAC TCCATCCCCA
    CGGGAAGAGA TGCAACCCAT GCTGCAAGCT CACCTGGATG AGTTGACAAG AGCCTATCAG
    TTTGATATAG CGAAGCAAAC ATGGCACATT CAAAGCAATA ATCAACCTCC ACAGCCCCCG
    GTTCCACCAT TAGGTTACAT GTCCGGAGCC TTGATTTCCG ATTTGGAAAC AGATGTTCCA
    GATGATGACG CTGATGATGA GGAGGAAGCT TTAGAAATCC CCAGACCCCT GAGAGCACTA
    GAGCAGACAC CCGGATCCAG TATGGACAAT CTAGACAGCT CTGTGACAGG CAAAGCTTTT
    ACCTCCTCTC AAAGACCTCG ACCAACCAGC CCATTTTCTA CTGACAGTAA TACCAGTGCA
    GCCCTGAGTC AAAATCAGAG GCCTCGGCCC ACTAAAAAAC ACAAGGGAGG GCGAATGGAT
    CAACAACCAG CATTGCCTCA TCGAAGGGAA GGAATGACAG ATGAGGAAAC ATTGGTGCCA
    TACAGCAAGC CCAGTTTCCC ATCTCCGGGA GGTCACAGCT CATCAGGAAC AGCATCTTCT
    AAAGGATCCA CTGGACCTAG GAAAGCCGAG GCGTTGAGGG GAAGTCACCA ACGCAATGCC
    AGTGACCTTC TTGACATAGG ATATATGGGC TCAAATAGTC AAGGACAGTT TACAGGTGAA
    TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965213.1
    CDS584..4789
    Translation

    Target ORF information:

    RefSeq Version XM_014109738.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109738.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCCTCC ACAGTTTGTG
    GTTAGGCCAA GAGATCAGAT TGTTGCTCAA GGTCGAACAG TTACATTTCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAAAAAGAAG GCAGCCAGAA CTTGCTTTTT
    CCAAATCAAC CCCAGCAACC CAACAGTAGA TGCTCTGTTT CGCCAACTGG AGACCTCACA
    ATTACCAACA TTCAGCGCTC CGATGCGGGT TACTATATCT GCCAGGCATT AACTGTGGCA
    GGAAGCATTT TAGCAAAAGC TCAACTGGAA GTTACTGATG TTTTAACAGA TAGACCTCCA
    CCTATAATTC TACAAGGCCC TGCAAACCAA ACTCTAGCAG TTGATGGTAC AGCATTACTG
    AAATGTAAAG CTACTGGGGA TCCTCTTCCT GTAATTAGCT GGTTAAAGGA GGGATTTACT
    TTTCTGGGTA GAGATTCAAG AGCAACTATA CAAGAACAAG GAACATTGCA GATTAAGAAT
    TTACGGATTT CTGATACTGG TACTTATACT TGTGTGGCTA CAAGTTCAAG TGGGGAGACT
    TCGTGGAGTG CTGTGCTGGA TGTGACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAC
    TTAAGTGACC TACCAGGGCC ACCATCCAAA CCACAGGTCA CTGATGTTAC TAAGAACAGT
    GTCACCTTGT CCTGGCAACC AGGTACCCCT GGAGCCCTTC CAGCAAGTGC ATATATCATT
    GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACGG TGGCAAACCA TGTAAAGACC
    ACGCTCTATA CAGTGAGAGG ATTGCGGCCC AATACGATCT ATTTATTCAT GGTCAGAGCT
    ATCAACCCCC AAGGTCTAAG CGACCCAAGC CCCATGTCAG ATCCTGTGCG CACACAAGAT
    ATCAGCCCAC CAGCACAAGG AGTGGACCAT AGACAAGTAC AGAAAGAACT AGGAGATGTC
    ATTGTCCGTC TTCATACTCC AGTTGTGTTG ACTCCCACCA CTGTTCAGGT CACATGGACG
    GTTGATCGCC AGCCCCAGTT TATTCAAGGC TACCGAGTGA TGTATCGTCA GACCACAGGC
    CTGCAGGCTA CGACAACATG GCAGAATTTA GATGCCAAAG TCCCAACCGA GCGAAGTGCT
    GTCTTGGTCA ACCTGAAAAA GGGAGTGACT TATGAAATTA AAGTTCGACC ATATTTTAAT
    GAGTTCCAAG GAATGGACAG TGAATCTAAA ATAGTTCGTA CTACTGAAGA AGCCCCAAGT
    GCCCCTCCAC AATCTGTCAC TGTGCTGACA GTTGGAAGCC ACAATAGCAC AAGTATTAGT
    GTTTCCTGGG ATCCTCCTCC TCCAGATCAT CAGAATGGAA TCATCCAAGA ATACAAGATC
    TGGTGTCTGG GGAATGAAAC ACGATTCCAT ATCAACAAAA CTGTGGATGC AGCCATTCGA
    TCTGTGATAA TTGGTGGATT GTTCCCAGGT ATTCAGTACC GGGTAGAGGT TGCAGCTAGT
    ACAAGTGCAG GGGTTGGAGT AAAAAGTGAG CCGCAGCCGA TAATAATTGG GGGACGTAAT
    GAAGTTGTCA TTACTGAAAA CAATAACAGC ATAACTGAGC AAATCACTGA TGTTGTGAAG
    CAACCAGCCT TTATAGCTGG AATTGGTGGT GCCTGCTGGG TAATTTTGAT GGGTTTTAGC
    ATCTGGTTGT ATTGGCGAAG AAAGAAGAGA AAGGGACTCA GTAACTATGC TGTTACATTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CTGGTCTTCT AAATGCTGGT
    GATCCCAGTT ACCCATGGCT TGCTGATTCA TGGCCAGCCA CGAGCTTACC AGTAAACAAT
    AGCAATAGTG GCCCAAATGA GATTGGGAAT TTTGGACGTG GAGATGTGCT GCCACCAGTT
    CCAGGCCAAG GGGATAAAAC AGCAACGATG CTCTCAGATG GAGCCATTTA TAGCAGCATT
    GACTTCACGA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC TACCCCATAT
    GCCACAACAC AGATCTTGCA TTCCAATAGC ATACATGAAT TGGCTGTCGA TCTGCCGGAC
    CCACAGTGGA AGAGCTCAAT TCAGCAAAAA ACGGACCTGA TGGGCTTTGG ATATTCTCTC
    CCTGATCAGA ACAAGGGTAA CAACGGTGGG AAAGGTGGAA AAAAGAAGAA AACTAAAAAC
    TCTTCTAAAC CACAGAAAAA CAATGGATCG ACTTGGGCCA ATGTACCCCT ACCTCCTCCC
    CCAGTCCAGC CCCTTCCTGG TACAGAGCTG GAACACTATG CAGTGGAACC GCAAGAAAAT
    GGGTATGACA GTGATAGCTG GTGCCCGCCA TTACCGGTAC AAACATACTT ACACCAGGGT
    ATGGAAGATG AACTGGAAGA AGATGATGAT CGTGTCCCAA CACCTCCTGT CCGAGGCGTG
    GCTTCTTCTC CTGCTATTTC CTTCGGACAG CAGTCCACTG CAACTCTTAC TCCATCCCCA
    CGGGAAGAGA TGCAACCCAT GCTGCAAGCT CACCTGGATG AGTTGACAAG AGCCTATCAG
    TTTGATATAG CGAAGCAAAC ATGGCACATT CAAAGCAATA ATCAACCTCC ACAGCCCCCG
    GTTCCACCAT TAGGTTACAT GTCCGGAGCC TTGATTTCCG ATTTGGAAAC AGATGTTCCA
    GATGATGACG CTGATGATGA GGAGGAAGCT TTAGAAATCC CCAGACCCCT GAGAGCACTA
    GAGCAGACAC CCGGATCCAG TATGGACAAT CTAGACAGCT CTGTGACAGG CAAAGCTTTT
    ACCTCCTCTC AAAGACCTCG ACCAACCAGC CCATTTTCTA CTGACAGTAA TACCAGTGCA
    GCCCTGAGTC AAAATCAGAG GCCTCGGCCC ACTAAAAAAC ACAAGGGAGG GCGAATGGAT
    CAACAACCAG CATTGCCTCA TCGAAGGGAA GGAATGACAG ATGAGGAAAC ATTGGTGCCA
    TACAGCAAGC CCAGTTTCCC ATCTCCGGGA GGTCACAGCT CATCAGGAAC AGCATCTTCT
    AAAGGATCCA CTGGACCTAG GAAAGCCGAG GCGTTGAGGG GAAGTCACCA ACGCAATGCC
    AGTGACCTTC TTGACATAGG ATATATGGGC TCAAATAGTC AAGGACAGTT TACAGGTGAA
    TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44457
    Clone ID Related Accession (Same CDS sequence) XM_014109739.1
    Accession Version XM_014109739.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4158bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product roundabout homolog 2 isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTTACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAAAAAGA AGGCAGCCAG
    AACTTGCTTT TTCCAAATCA ACCCCAGCAA CCCAACAGTA GATGCTCTGT TTCGCCAACT
    GGAGACCTCA CAATTACCAA CATTCAGCGC TCCGATGCGG GTTACTATAT CTGCCAGGCA
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AAGTTACTGA TGTTTTAACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCTGCAAACC AAACTCTAGC AGTTGATGGT
    ACAGCATTAC TGAAATGTAA AGCTACTGGG GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCTGGG TAGAGATTCA AGAGCAACTA TACAAGAACA AGGAACATTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGTACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGGGAGA CTTCGTGGAG TGCTGTGCTG GATGTGACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ACTTAAGTGA CCTACCAGGG CCACCATCCA AACCACAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAA CCAGGTACCC CTGGAGCCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC GGTGGCAAAC
    CATGTAAAGA CCACGCTCTA TACAGTGAGA GGATTGCGGC CCAATACGAT CTATTTATTC
    ATGGTCAGAG CTATCAACCC CCAAGGTCTA AGCGACCCAA GCCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ATAGACAAGT ACAGAAAGAA
    CTAGGAGATG TCATTGTCCG TCTTCATACT CCAGTTGTGT TGACTCCCAC CACTGTTCAG
    GTCACATGGA CGGTTGATCG CCAGCCCCAG TTTATTCAAG GCTACCGAGT GATGTATCGT
    CAGACCACAG GCCTGCAGGC TACGACAACA TGGCAGAATT TAGATGCCAA AGTCCCAACC
    GAGCGAAGTG CTGTCTTGGT CAACCTGAAA AAGGGAGTGA CTTATGAAAT TAAAGTTCGA
    CCATATTTTA ATGAGTTCCA AGGAATGGAC AGTGAATCTA AAATAGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCTCC ACAATCTGTC ACTGTGCTGA CAGTTGGAAG CCACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ATCAGAATGG AATCATCCAA
    GAATACAAGA TCTGGTGTCT GGGGAATGAA ACACGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GATCTGTGAT AATTGGTGGA TTGTTCCCAG GTATTCAGTA CCGGGTAGAG
    GTTGCAGCTA GTACAAGTGC AGGGGTTGGA GTAAAAAGTG AGCCGCAGCC GATAATAATT
    GGGGGACGTA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTTGTGA AGCAACCAGC CTTTATAGCT GGAATTGGTG GTGCCTGCTG GGTAATTTTG
    ATGGGTTTTA GCATCTGGTT GTATTGGCGA AGAAAGAAGA GAAAGGGACT CAGTAACTAT
    GCTGTTACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCTGGTCTT
    CTAAATGCTG GTGATCCCAG TTACCCATGG CTTGCTGATT CATGGCCAGC CACGAGCTTA
    CCAGTAAACA ATAGCAATAG TGGCCCAAAT GAGATTGGGA ATTTTGGACG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC GACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACAAC ACAGATCTTG CATTCCAATA GCATACATGA ATTGGCTGTC
    GATCTGCCGG ACCCACAGTG GAAGAGCTCA ATTCAGCAAA AAACGGACCT GATGGGCTTT
    GGATATTCTC TCCCTGATCA GAACAAGGGT AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAGAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCGAC TTGGGCCAAT
    GTACCCCTAC CTCCTCCCCC AGTCCAGCCC CTTCCTGGTA CAGAGCTGGA ACACTATGCA
    GTGGAACCGC AAGAAAATGG GTATGACAGT GATAGCTGGT GCCCGCCATT ACCGGTACAA
    ACATACTTAC ACCAGGGTAT GGAAGATGAA CTGGAAGAAG ATGATGATCG TGTCCCAACA
    CCTCCTGTCC GAGGCGTGGC TTCTTCTCCT GCTATTTCCT TCGGACAGCA GTCCACTGCA
    ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAAGCTCA CCTGGATGAG
    TTGACAAGAG CCTATCAGTT TGATATAGCG AAGCAAACAT GGCACATTCA AAGCAATAAT
    CAACCTCCAC AGCCCCCGGT TCCACCATTA GGTTACATGT CCGGAGCCTT GATTTCCGAT
    TTGGAAACAG ATGTTCCAGA TGATGACGCT GATGATGAGG AGGAAGCTTT AGAAATCCCC
    AGACCCCTGA GAGCACTAGA GCAGACACCC GGATCCAGTA TGGACAATCT AGACAGCTCT
    GTGACAGGTT CAATGGTAAA TGGCTGGGGC TCTGCATCTG ATGAGGATCG TAACTTTTCT
    AGTCATAGAT CTAGTGTAGG TAGCTCCTCA GATGGCTCCA TCTTTGCCAG CGGCAGCTTT
    GCACAAGCAG TGGTGGCAGC AGCAGATAAA GCTGGTTTTA GGCTGGATGG AACCAGCCTT
    ACAAGAACAG GTTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965214.1
    CDS589..4746
    Translation

    Target ORF information:

    RefSeq Version XM_014109739.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109739.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTTACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAAAAAGA AGGCAGCCAG
    AACTTGCTTT TTCCAAATCA ACCCCAGCAA CCCAACAGTA GATGCTCTGT TTCGCCAACT
    GGAGACCTCA CAATTACCAA CATTCAGCGC TCCGATGCGG GTTACTATAT CTGCCAGGCA
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AAGTTACTGA TGTTTTAACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCTGCAAACC AAACTCTAGC AGTTGATGGT
    ACAGCATTAC TGAAATGTAA AGCTACTGGG GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCTGGG TAGAGATTCA AGAGCAACTA TACAAGAACA AGGAACATTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGTACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGGGAGA CTTCGTGGAG TGCTGTGCTG GATGTGACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ACTTAAGTGA CCTACCAGGG CCACCATCCA AACCACAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAA CCAGGTACCC CTGGAGCCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC GGTGGCAAAC
    CATGTAAAGA CCACGCTCTA TACAGTGAGA GGATTGCGGC CCAATACGAT CTATTTATTC
    ATGGTCAGAG CTATCAACCC CCAAGGTCTA AGCGACCCAA GCCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ATAGACAAGT ACAGAAAGAA
    CTAGGAGATG TCATTGTCCG TCTTCATACT CCAGTTGTGT TGACTCCCAC CACTGTTCAG
    GTCACATGGA CGGTTGATCG CCAGCCCCAG TTTATTCAAG GCTACCGAGT GATGTATCGT
    CAGACCACAG GCCTGCAGGC TACGACAACA TGGCAGAATT TAGATGCCAA AGTCCCAACC
    GAGCGAAGTG CTGTCTTGGT CAACCTGAAA AAGGGAGTGA CTTATGAAAT TAAAGTTCGA
    CCATATTTTA ATGAGTTCCA AGGAATGGAC AGTGAATCTA AAATAGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCTCC ACAATCTGTC ACTGTGCTGA CAGTTGGAAG CCACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ATCAGAATGG AATCATCCAA
    GAATACAAGA TCTGGTGTCT GGGGAATGAA ACACGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GATCTGTGAT AATTGGTGGA TTGTTCCCAG GTATTCAGTA CCGGGTAGAG
    GTTGCAGCTA GTACAAGTGC AGGGGTTGGA GTAAAAAGTG AGCCGCAGCC GATAATAATT
    GGGGGACGTA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTTGTGA AGCAACCAGC CTTTATAGCT GGAATTGGTG GTGCCTGCTG GGTAATTTTG
    ATGGGTTTTA GCATCTGGTT GTATTGGCGA AGAAAGAAGA GAAAGGGACT CAGTAACTAT
    GCTGTTACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCTGGTCTT
    CTAAATGCTG GTGATCCCAG TTACCCATGG CTTGCTGATT CATGGCCAGC CACGAGCTTA
    CCAGTAAACA ATAGCAATAG TGGCCCAAAT GAGATTGGGA ATTTTGGACG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC GACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACAAC ACAGATCTTG CATTCCAATA GCATACATGA ATTGGCTGTC
    GATCTGCCGG ACCCACAGTG GAAGAGCTCA ATTCAGCAAA AAACGGACCT GATGGGCTTT
    GGATATTCTC TCCCTGATCA GAACAAGGGT AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAGAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCGAC TTGGGCCAAT
    GTACCCCTAC CTCCTCCCCC AGTCCAGCCC CTTCCTGGTA CAGAGCTGGA ACACTATGCA
    GTGGAACCGC AAGAAAATGG GTATGACAGT GATAGCTGGT GCCCGCCATT ACCGGTACAA
    ACATACTTAC ACCAGGGTAT GGAAGATGAA CTGGAAGAAG ATGATGATCG TGTCCCAACA
    CCTCCTGTCC GAGGCGTGGC TTCTTCTCCT GCTATTTCCT TCGGACAGCA GTCCACTGCA
    ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAAGCTCA CCTGGATGAG
    TTGACAAGAG CCTATCAGTT TGATATAGCG AAGCAAACAT GGCACATTCA AAGCAATAAT
    CAACCTCCAC AGCCCCCGGT TCCACCATTA GGTTACATGT CCGGAGCCTT GATTTCCGAT
    TTGGAAACAG ATGTTCCAGA TGATGACGCT GATGATGAGG AGGAAGCTTT AGAAATCCCC
    AGACCCCTGA GAGCACTAGA GCAGACACCC GGATCCAGTA TGGACAATCT AGACAGCTCT
    GTGACAGGTT CAATGGTAAA TGGCTGGGGC TCTGCATCTG ATGAGGATCG TAACTTTTCT
    AGTCATAGAT CTAGTGTAGG TAGCTCCTCA GATGGCTCCA TCTTTGCCAG CGGCAGCTTT
    GCACAAGCAG TGGTGGCAGC AGCAGATAAA GCTGGTTTTA GGCTGGATGG AACCAGCCTT
    ACAAGAACAG GTTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44458
    Clone ID Related Accession (Same CDS sequence) XM_014109740.1
    Accession Version XM_014109740.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product roundabout homolog 2 isoform X11
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCCTCC ACAGTTTGTG
    GTTAGGCCAA GAGATCAGAT TGTTGCTCAA GGTCGAACAG TTACATTTCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAAAAAGAAG GCAGCCAGAA CTTGCTTTTT
    CCAAATCAAC CCCAGCAACC CAACAGTAGA TGCTCTGTTT CGCCAACTGG AGACCTCACA
    ATTACCAACA TTCAGCGCTC CGATGCGGGT TACTATATCT GCCAGGCATT AACTGTGGCA
    GGAAGCATTT TAGCAAAAGC TCAACTGGAA GTTACTGATG TTTTAACAGA TAGACCTCCA
    CCTATAATTC TACAAGGCCC TGCAAACCAA ACTCTAGCAG TTGATGGTAC AGCATTACTG
    AAATGTAAAG CTACTGGGGA TCCTCTTCCT GTAATTAGCT GGTTAAAGGA GGGATTTACT
    TTTCTGGGTA GAGATTCAAG AGCAACTATA CAAGAACAAG GAACATTGCA GATTAAGAAT
    TTACGGATTT CTGATACTGG TACTTATACT TGTGTGGCTA CAAGTTCAAG TGGGGAGACT
    TCGTGGAGTG CTGTGCTGGA TGTGACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAC
    TTAAGTGACC TACCAGGGCC ACCATCCAAA CCACAGGTCA CTGATGTTAC TAAGAACAGT
    GTCACCTTGT CCTGGCAACC AGGTACCCCT GGAGCCCTTC CAGCAAGTGC ATATATCATT
    GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACGG TGGCAAACCA TGTAAAGACC
    ACGCTCTATA CAGTGAGAGG ATTGCGGCCC AATACGATCT ATTTATTCAT GGTCAGAGCT
    ATCAACCCCC AAGGTCTAAG CGACCCAAGC CCCATGTCAG ATCCTGTGCG CACACAAGAT
    ATCAGCCCAC CAGCACAAGG AGTGGACCAT AGACAAGTAC AGAAAGAACT AGGAGATGTC
    ATTGTCCGTC TTCATACTCC AGTTGTGTTG ACTCCCACCA CTGTTCAGGT CACATGGACG
    GTTGATCGCC AGCCCCAGTT TATTCAAGGC TACCGAGTGA TGTATCGTCA GACCACAGGC
    CTGCAGGCTA CGACAACATG GCAGAATTTA GATGCCAAAG TCCCAACCGA GCGAAGTGCT
    GTCTTGGTCA ACCTGAAAAA GGGAGTGACT TATGAAATTA AAGTTCGACC ATATTTTAAT
    GAGTTCCAAG GAATGGACAG TGAATCTAAA ATAGTTCGTA CTACTGAAGA AGCCCCAAGT
    GCCCCTCCAC AATCTGTCAC TGTGCTGACA GTTGGAAGCC ACAATAGCAC AAGTATTAGT
    GTTTCCTGGG ATCCTCCTCC TCCAGATCAT CAGAATGGAA TCATCCAAGA ATACAAGATC
    TGGTGTCTGG GGAATGAAAC ACGATTCCAT ATCAACAAAA CTGTGGATGC AGCCATTCGA
    TCTGTGATAA TTGGTGGATT GTTCCCAGGT ATTCAGTACC GGGTAGAGGT TGCAGCTAGT
    ACAAGTGCAG GGGTTGGAGT AAAAAGTGAG CCGCAGCCGA TAATAATTGG GGGACGTAAT
    GAAGTTGTCA TTACTGAAAA CAATAACAGC ATAACTGAGC AAATCACTGA TGTTGTGAAG
    CAACCAGCCT TTATAGCTGG AATTGGTGGT GCCTGCTGGG TAATTTTGAT GGGTTTTAGC
    ATCTGGTTGT ATTGGCGAAG AAAGAAGAGA AAGGGACTCA GTAACTATGC TGTTACATTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CTGGTCTTCT AAATGCTGGT
    GATCCCAGTT ACCCATGGCT TGCTGATTCA TGGCCAGCCA CGAGCTTACC AGTAAACAAT
    AGCAATAGTG GCCCAAATGA GATTGGGAAT TTTGGACGTG GAGATGTGCT GCCACCAGTT
    CCAGGCCAAG GGGATAAAAC AGCAACGATG CTCTCAGATG GAGCCATTTA TAGCAGCATT
    GACTTCACGA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC TACCCCATAT
    GCCACAACAC AGATCTTGCA TTCCAATAGC ATACATGAAT TGGCTGTCGA TCTGCCGGAC
    CCACAGTGGA AGAGCTCAAT TCAGCAAAAA ACGGACCTGA TGGGCTTTGG ATATTCTCTC
    CCTGATCAGA ACAAGGGTAA CAACGCCTTA CTTTACATCC CTGACTACCG ATTGGCTGAG
    GGATTGTCTA ATAGAATGCC ACACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC
    AACAGCTCAG AGAGGAGTGG CAGTCTCTCA GGTGGGAAAG GTGGAAAAAA GAAGAAAACT
    AAAAACTCTT CTAAACCACA GAAAAACAAT GGATCGACTT GGGCCAATGT ACCCCTACCT
    CCTCCCCCAG TCCAGCCCCT TCCTGGTACA GAGCTGGAAC ACTATGCAGT GGAACCGCAA
    GAAAATGGGT ATGACAGTGA TAGCTGGTGC CCGCCATTAC CGGTACAAAC ATACTTACAC
    CAGGGTATGG AAGATGAACT GGAAGAAGAT GATGATCGTG TCCCAACACC TCCTGTCCGA
    GGCGTGGCTT CTTCTCCTGC TATTTCCTTC GGACAGCAGT CCACTGCAAC TCTTACTCCA
    TCCCCACGGG AAGAGATGCA ACCCATGCTG CAAGCTCACC TGGATGAGTT GACAAGAGCC
    TATCAGTTTG ATATAGCGAA GCAAACATGG CACATTCAAA GCAATAATCA ACCTCCACAG
    CCCCCGGTTC CACCATTAGG TTACATGTCC GGAGCCTTGA TTTCCGATTT GGAAACAGAT
    GTTCCAGATG ATGACGCTGA TGATGAGGAG GAAGCTTTAG AAATCCCCAG ACCCCTGAGA
    GCACTAGAGC AGACACCCGG ATCCAGTATG GACAATCTAG ACAGCTCTGT GACAGGTTCA
    ATGGTAAATG GCTGGGGCTC TGCATCTGAT GAGGATCGTA ACTTTTCTAG TCATAGATCT
    AGTGTAGGTA GCTCCTCAGA TGGCTCCATC TTTGCCAGCG GCAGCTTTGC ACAAGCAGTG
    GTGGCAGCAG CAGATAAAGC TGGTTTTAGG CTGGATGGAA CCAGCCTTAC AAGAACAGGT
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965215.1
    CDS590..4735
    Translation

    Target ORF information:

    RefSeq Version XM_014109740.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109740.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCCTCC ACAGTTTGTG
    GTTAGGCCAA GAGATCAGAT TGTTGCTCAA GGTCGAACAG TTACATTTCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAAAAAGAAG GCAGCCAGAA CTTGCTTTTT
    CCAAATCAAC CCCAGCAACC CAACAGTAGA TGCTCTGTTT CGCCAACTGG AGACCTCACA
    ATTACCAACA TTCAGCGCTC CGATGCGGGT TACTATATCT GCCAGGCATT AACTGTGGCA
    GGAAGCATTT TAGCAAAAGC TCAACTGGAA GTTACTGATG TTTTAACAGA TAGACCTCCA
    CCTATAATTC TACAAGGCCC TGCAAACCAA ACTCTAGCAG TTGATGGTAC AGCATTACTG
    AAATGTAAAG CTACTGGGGA TCCTCTTCCT GTAATTAGCT GGTTAAAGGA GGGATTTACT
    TTTCTGGGTA GAGATTCAAG AGCAACTATA CAAGAACAAG GAACATTGCA GATTAAGAAT
    TTACGGATTT CTGATACTGG TACTTATACT TGTGTGGCTA CAAGTTCAAG TGGGGAGACT
    TCGTGGAGTG CTGTGCTGGA TGTGACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAC
    TTAAGTGACC TACCAGGGCC ACCATCCAAA CCACAGGTCA CTGATGTTAC TAAGAACAGT
    GTCACCTTGT CCTGGCAACC AGGTACCCCT GGAGCCCTTC CAGCAAGTGC ATATATCATT
    GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACGG TGGCAAACCA TGTAAAGACC
    ACGCTCTATA CAGTGAGAGG ATTGCGGCCC AATACGATCT ATTTATTCAT GGTCAGAGCT
    ATCAACCCCC AAGGTCTAAG CGACCCAAGC CCCATGTCAG ATCCTGTGCG CACACAAGAT
    ATCAGCCCAC CAGCACAAGG AGTGGACCAT AGACAAGTAC AGAAAGAACT AGGAGATGTC
    ATTGTCCGTC TTCATACTCC AGTTGTGTTG ACTCCCACCA CTGTTCAGGT CACATGGACG
    GTTGATCGCC AGCCCCAGTT TATTCAAGGC TACCGAGTGA TGTATCGTCA GACCACAGGC
    CTGCAGGCTA CGACAACATG GCAGAATTTA GATGCCAAAG TCCCAACCGA GCGAAGTGCT
    GTCTTGGTCA ACCTGAAAAA GGGAGTGACT TATGAAATTA AAGTTCGACC ATATTTTAAT
    GAGTTCCAAG GAATGGACAG TGAATCTAAA ATAGTTCGTA CTACTGAAGA AGCCCCAAGT
    GCCCCTCCAC AATCTGTCAC TGTGCTGACA GTTGGAAGCC ACAATAGCAC AAGTATTAGT
    GTTTCCTGGG ATCCTCCTCC TCCAGATCAT CAGAATGGAA TCATCCAAGA ATACAAGATC
    TGGTGTCTGG GGAATGAAAC ACGATTCCAT ATCAACAAAA CTGTGGATGC AGCCATTCGA
    TCTGTGATAA TTGGTGGATT GTTCCCAGGT ATTCAGTACC GGGTAGAGGT TGCAGCTAGT
    ACAAGTGCAG GGGTTGGAGT AAAAAGTGAG CCGCAGCCGA TAATAATTGG GGGACGTAAT
    GAAGTTGTCA TTACTGAAAA CAATAACAGC ATAACTGAGC AAATCACTGA TGTTGTGAAG
    CAACCAGCCT TTATAGCTGG AATTGGTGGT GCCTGCTGGG TAATTTTGAT GGGTTTTAGC
    ATCTGGTTGT ATTGGCGAAG AAAGAAGAGA AAGGGACTCA GTAACTATGC TGTTACATTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CTGGTCTTCT AAATGCTGGT
    GATCCCAGTT ACCCATGGCT TGCTGATTCA TGGCCAGCCA CGAGCTTACC AGTAAACAAT
    AGCAATAGTG GCCCAAATGA GATTGGGAAT TTTGGACGTG GAGATGTGCT GCCACCAGTT
    CCAGGCCAAG GGGATAAAAC AGCAACGATG CTCTCAGATG GAGCCATTTA TAGCAGCATT
    GACTTCACGA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC TACCCCATAT
    GCCACAACAC AGATCTTGCA TTCCAATAGC ATACATGAAT TGGCTGTCGA TCTGCCGGAC
    CCACAGTGGA AGAGCTCAAT TCAGCAAAAA ACGGACCTGA TGGGCTTTGG ATATTCTCTC
    CCTGATCAGA ACAAGGGTAA CAACGCCTTA CTTTACATCC CTGACTACCG ATTGGCTGAG
    GGATTGTCTA ATAGAATGCC ACACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC
    AACAGCTCAG AGAGGAGTGG CAGTCTCTCA GGTGGGAAAG GTGGAAAAAA GAAGAAAACT
    AAAAACTCTT CTAAACCACA GAAAAACAAT GGATCGACTT GGGCCAATGT ACCCCTACCT
    CCTCCCCCAG TCCAGCCCCT TCCTGGTACA GAGCTGGAAC ACTATGCAGT GGAACCGCAA
    GAAAATGGGT ATGACAGTGA TAGCTGGTGC CCGCCATTAC CGGTACAAAC ATACTTACAC
    CAGGGTATGG AAGATGAACT GGAAGAAGAT GATGATCGTG TCCCAACACC TCCTGTCCGA
    GGCGTGGCTT CTTCTCCTGC TATTTCCTTC GGACAGCAGT CCACTGCAAC TCTTACTCCA
    TCCCCACGGG AAGAGATGCA ACCCATGCTG CAAGCTCACC TGGATGAGTT GACAAGAGCC
    TATCAGTTTG ATATAGCGAA GCAAACATGG CACATTCAAA GCAATAATCA ACCTCCACAG
    CCCCCGGTTC CACCATTAGG TTACATGTCC GGAGCCTTGA TTTCCGATTT GGAAACAGAT
    GTTCCAGATG ATGACGCTGA TGATGAGGAG GAAGCTTTAG AAATCCCCAG ACCCCTGAGA
    GCACTAGAGC AGACACCCGG ATCCAGTATG GACAATCTAG ACAGCTCTGT GACAGGTTCA
    ATGGTAAATG GCTGGGGCTC TGCATCTGAT GAGGATCGTA ACTTTTCTAG TCATAGATCT
    AGTGTAGGTA GCTCCTCAGA TGGCTCCATC TTTGCCAGCG GCAGCTTTGC ACAAGCAGTG
    GTGGCAGCAG CAGATAAAGC TGGTTTTAGG CTGGATGGAA CCAGCCTTAC AAGAACAGGT
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe283873
    Clone ID Related Accession (Same CDS sequence) XM_022413096.1
    Accession Version XM_022413096.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product roundabout homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTTACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAAAAAGA AGGCAGCCAG
    AACTTGCTTT TTCCAAATCA ACCCCAGCAA CCCAACAGTA GATGCTCTGT TTCGCCAACT
    GGAGACCTCA CAATTACCAA CATTCAGCGC TCCGATGCGG GTTACTATAT CTGCCAGGCA
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AAGTTACTGA TGTTTTAACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCTGCAAACC AAACTCTAGC AGTTGATGGT
    ACAGCATTAC TGAAATGTAA AGCTACTGGG GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCTGGG TAGAGATTCA AGAGCAACTA TACAAGAACA AGGAACATTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGTACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGGGAGA CTTCGTGGAG TGCTGTGCTG GATGTGACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ACTTAAGTGA CCTACCAGGG CCACCATCCA AACCACAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAA CCAGGTACCC CTGGAGCCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC GGTGGCAAAC
    CATGTAAAGA CCACGCTCTA TACAGTGAGA GGATTGCGGC CCAATACGAT CTATTTATTC
    ATGGTCAGAG CTATCAACCC CCAAGGTCTA AGCGACCCAA GCCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ATAGACAAGT ACAGAAAGAA
    CTAGGAGATG TCATTGTCCG TCTTCATACT CCAGTTGTGT TGACTCCCAC CACTGTTCAG
    GTCACATGGA CGGTTGATCG CCAGCCCCAG TTTATTCAAG GCTACCGAGT GATGTATCGT
    CAGACCACAG GCCTGCAGGC TACGACAACA TGGCAGAATT TAGATGCCAA AGTCCCAACC
    GAGCGAAGTG CTGTCTTGGT CAACCTGAAA AAGGGAGTGA CTTATGAAAT TAAAGTTCGA
    CCATATTTTA ATGAGTTCCA AGGAATGGAC AGTGAATCTA AAATAGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCTCC ACAATCTGTC ACTGTGCTGA CAGTTGGAAG CCACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ATCAGAATGG AATCATCCAA
    GAATACAAGA TCTGGTGTCT GGGGAATGAA ACACGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GATCTGTGAT AATTGGTGGA TTGTTCCCAG GTATTCAGTA CCGGGTAGAG
    GTTGCAGCTA GTACAAGTGC AGGGGTTGGA GTAAAAAGTG AGCCGCAGCC GATAATAATT
    GGGGGACGTA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTTGTGA AGCAACCAGC CTTTATAGCT GGAATTGGTG GTGCCTGCTG GGTAATTTTG
    ATGGGTTTTA GCATCTGGTT GTATTGGCGA AGAAAGAAGA GAAAGGGACT CAGTAACTAT
    GCTGTTACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCTGGTCTT
    CTAAATGCTG GTGATCCCAG TTACCCATGG CTTGCTGATT CATGGCCAGC CACGAGCTTA
    CCAGTAAACA ATAGCAATAG TGGCCCAAAT GAGATTGGGA ATTTTGGACG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC GACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACAAC ACAGATCTTG CATTCCAATA GCATACATGA ATTGGCTGTC
    GATCTGCCGG ACCCACAGTG GAAGAGCTCA ATTCAGCAAA AAACGGACCT GATGGGCTTT
    GGATATTCTC TCCCTGATCA GAACAAGGGT AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAGAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCGAC TTGGGCCAAT
    GTACCCCTAC CTCCTCCCCC AGTCCAGCCC CTTCCTGGTA CAGAGCTGGA ACACTATGCA
    GTGGAACCGC AAGAAAATGG GTATGACAGT GATAGCTGGT GCCCGCCATT ACCGGTACAA
    ACATACTTAC ACCAGGGTAT GGAAGATGAA CTGGAAGAAG ATGATGATCG TGTCCCAACA
    CCTCCTGTCC GAGGCGTGGC TTCTTCTCCT GCTATTTCCT TCGGACAGCA GTCCACTGCA
    ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAAGCTCA CCTGGATGAG
    TTGACAAGAG CCTATCAGTT TGATATAGCG AAGCAAACAT GGCACATTCA AAGCAATAAT
    CAACCTCCAC AGCCCCCGGT TCCACCATTA GGTTACATGT CCGGAGCCTT GATTTCCGAT
    TTGGAAACAG ATGTTCCAGA TGATGACGCT GATGATGAGG AGGAAGCTTT AGAAATCCCC
    AGACCCCTGA GAGCACTAGA GCAGACACCC GGATCCAGTA TGGACAATCT AGACAGCTCT
    GTGACAGGTT CAATGGTAAA TGGCTGGGGC TCTGCATCTG ATGAGGATCG TAACTTTTCT
    AGTCATAGAT CTAGTGTAGG TAGCTCCTCA GATGGCTCCA TCTTTGCCAG CGGCAGCTTT
    GCACAAGCAG TGGTGGCAGC AGCAGATAAA GCTGGTTTTA GGCTGGATGG AACCAGCCTT
    ACAAGAACAG GCAAAGCTTT TACCTCCTCT CAAAGACCTC GACCAACCAG CCCATTTTCT
    ACTGACAGTA ATACCAGTGC AGCCCTGAGT CAAAATCAGA GGCCTCGGCC CACTAAAAAA
    CACAAGGGAG GGCGAATGGA TCAACAACCA GCATTGCCTC ATCGAAGGGA AGGAATGACA
    GATGATCTTC CACCACCACC AGATCCCCCT CCAGGTCAGG GTTTAAGGCA GCAAATAGGC
    CCGAGCCAGC ATGCTGGTAA CATGGAAAAC TCAACAGAGA GAAAAGGAAG CTCTCTGGAG
    ACACCACAAG CATCCAACTT AGAAGACACA AAGAGCTCAT TGGATTGTCC AGCTAGAACG
    TCCCTAGAGT GGCAGCGACA AACCCAAGAA TGGATAAACT CCACAGAACG CCAAGAAGAT
    ATACGGAAAG CCCTACACAA ACAAAGTGTT GGACCAGAGG AAACATTGGT GCCATACAGC
    AAGCCCAGTT TCCCATCTCC GGGAGGTCAC AGCTCATCAG GAACAGCATC TTCTAAAGGA
    TCCACTGGAC CTAGGAAAGC CGAGGCGTTG AGGGGAAGTC ACCAACGCAA TGCCAGTGAC
    CTTCTTGACA TAGGATATAT GGGCTCAAAT AGTCAAGGAC AGTTTACAGG TGAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268804.1
    CDS388..5187
    Translation

    Target ORF information:

    RefSeq Version XM_022413096.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413096.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTTACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAAAAAGA AGGCAGCCAG
    AACTTGCTTT TTCCAAATCA ACCCCAGCAA CCCAACAGTA GATGCTCTGT TTCGCCAACT
    GGAGACCTCA CAATTACCAA CATTCAGCGC TCCGATGCGG GTTACTATAT CTGCCAGGCA
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AAGTTACTGA TGTTTTAACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCTGCAAACC AAACTCTAGC AGTTGATGGT
    ACAGCATTAC TGAAATGTAA AGCTACTGGG GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCTGGG TAGAGATTCA AGAGCAACTA TACAAGAACA AGGAACATTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGTACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGGGAGA CTTCGTGGAG TGCTGTGCTG GATGTGACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ACTTAAGTGA CCTACCAGGG CCACCATCCA AACCACAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAA CCAGGTACCC CTGGAGCCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC GGTGGCAAAC
    CATGTAAAGA CCACGCTCTA TACAGTGAGA GGATTGCGGC CCAATACGAT CTATTTATTC
    ATGGTCAGAG CTATCAACCC CCAAGGTCTA AGCGACCCAA GCCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ATAGACAAGT ACAGAAAGAA
    CTAGGAGATG TCATTGTCCG TCTTCATACT CCAGTTGTGT TGACTCCCAC CACTGTTCAG
    GTCACATGGA CGGTTGATCG CCAGCCCCAG TTTATTCAAG GCTACCGAGT GATGTATCGT
    CAGACCACAG GCCTGCAGGC TACGACAACA TGGCAGAATT TAGATGCCAA AGTCCCAACC
    GAGCGAAGTG CTGTCTTGGT CAACCTGAAA AAGGGAGTGA CTTATGAAAT TAAAGTTCGA
    CCATATTTTA ATGAGTTCCA AGGAATGGAC AGTGAATCTA AAATAGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCTCC ACAATCTGTC ACTGTGCTGA CAGTTGGAAG CCACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ATCAGAATGG AATCATCCAA
    GAATACAAGA TCTGGTGTCT GGGGAATGAA ACACGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GATCTGTGAT AATTGGTGGA TTGTTCCCAG GTATTCAGTA CCGGGTAGAG
    GTTGCAGCTA GTACAAGTGC AGGGGTTGGA GTAAAAAGTG AGCCGCAGCC GATAATAATT
    GGGGGACGTA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTTGTGA AGCAACCAGC CTTTATAGCT GGAATTGGTG GTGCCTGCTG GGTAATTTTG
    ATGGGTTTTA GCATCTGGTT GTATTGGCGA AGAAAGAAGA GAAAGGGACT CAGTAACTAT
    GCTGTTACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCTGGTCTT
    CTAAATGCTG GTGATCCCAG TTACCCATGG CTTGCTGATT CATGGCCAGC CACGAGCTTA
    CCAGTAAACA ATAGCAATAG TGGCCCAAAT GAGATTGGGA ATTTTGGACG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC GACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACAAC ACAGATCTTG CATTCCAATA GCATACATGA ATTGGCTGTC
    GATCTGCCGG ACCCACAGTG GAAGAGCTCA ATTCAGCAAA AAACGGACCT GATGGGCTTT
    GGATATTCTC TCCCTGATCA GAACAAGGGT AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAGAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCGAC TTGGGCCAAT
    GTACCCCTAC CTCCTCCCCC AGTCCAGCCC CTTCCTGGTA CAGAGCTGGA ACACTATGCA
    GTGGAACCGC AAGAAAATGG GTATGACAGT GATAGCTGGT GCCCGCCATT ACCGGTACAA
    ACATACTTAC ACCAGGGTAT GGAAGATGAA CTGGAAGAAG ATGATGATCG TGTCCCAACA
    CCTCCTGTCC GAGGCGTGGC TTCTTCTCCT GCTATTTCCT TCGGACAGCA GTCCACTGCA
    ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAAGCTCA CCTGGATGAG
    TTGACAAGAG CCTATCAGTT TGATATAGCG AAGCAAACAT GGCACATTCA AAGCAATAAT
    CAACCTCCAC AGCCCCCGGT TCCACCATTA GGTTACATGT CCGGAGCCTT GATTTCCGAT
    TTGGAAACAG ATGTTCCAGA TGATGACGCT GATGATGAGG AGGAAGCTTT AGAAATCCCC
    AGACCCCTGA GAGCACTAGA GCAGACACCC GGATCCAGTA TGGACAATCT AGACAGCTCT
    GTGACAGGTT CAATGGTAAA TGGCTGGGGC TCTGCATCTG ATGAGGATCG TAACTTTTCT
    AGTCATAGAT CTAGTGTAGG TAGCTCCTCA GATGGCTCCA TCTTTGCCAG CGGCAGCTTT
    GCACAAGCAG TGGTGGCAGC AGCAGATAAA GCTGGTTTTA GGCTGGATGG AACCAGCCTT
    ACAAGAACAG GCAAAGCTTT TACCTCCTCT CAAAGACCTC GACCAACCAG CCCATTTTCT
    ACTGACAGTA ATACCAGTGC AGCCCTGAGT CAAAATCAGA GGCCTCGGCC CACTAAAAAA
    CACAAGGGAG GGCGAATGGA TCAACAACCA GCATTGCCTC ATCGAAGGGA AGGAATGACA
    GATGATCTTC CACCACCACC AGATCCCCCT CCAGGTCAGG GTTTAAGGCA GCAAATAGGC
    CCGAGCCAGC ATGCTGGTAA CATGGAAAAC TCAACAGAGA GAAAAGGAAG CTCTCTGGAG
    ACACCACAAG CATCCAACTT AGAAGACACA AAGAGCTCAT TGGATTGTCC AGCTAGAACG
    TCCCTAGAGT GGCAGCGACA AACCCAAGAA TGGATAAACT CCACAGAACG CCAAGAAGAT
    ATACGGAAAG CCCTACACAA ACAAAGTGTT GGACCAGAGG AAACATTGGT GCCATACAGC
    AAGCCCAGTT TCCCATCTCC GGGAGGTCAC AGCTCATCAG GAACAGCATC TTCTAAAGGA
    TCCACTGGAC CTAGGAAAGC CGAGGCGTTG AGGGGAAGTC ACCAACGCAA TGCCAGTGAC
    CTTCTTGACA TAGGATATAT GGGCTCAAAT AGTCAAGGAC AGTTTACAGG TGAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe283874
    Clone ID Related Accession (Same CDS sequence) XM_022413098.1
    Accession Version XM_022413098.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4788bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product roundabout homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCCTCC ACAGTTTGTG
    GTTAGGCCAA GAGATCAGAT TGTTGCTCAA GGTCGAACAG TTACATTTCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAAAAAGAAG GCAGCCAGAA CTTGCTTTTT
    CCAAATCAAC CCCAGCAACC CAACAGTAGA TGCTCTGTTT CGCCAACTGG AGACCTCACA
    ATTACCAACA TTCAGCGCTC CGATGCGGGT TACTATATCT GCCAGGCATT AACTGTGGCA
    GGAAGCATTT TAGCAAAAGC TCAACTGGAA GTTACTGATG TTTTAACAGA TAGACCTCCA
    CCTATAATTC TACAAGGCCC TGCAAACCAA ACTCTAGCAG TTGATGGTAC AGCATTACTG
    AAATGTAAAG CTACTGGGGA TCCTCTTCCT GTAATTAGCT GGTTAAAGGA GGGATTTACT
    TTTCTGGGTA GAGATTCAAG AGCAACTATA CAAGAACAAG GAACATTGCA GATTAAGAAT
    TTACGGATTT CTGATACTGG TACTTATACT TGTGTGGCTA CAAGTTCAAG TGGGGAGACT
    TCGTGGAGTG CTGTGCTGGA TGTGACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAC
    TTAAGTGACC TACCAGGGCC ACCATCCAAA CCACAGGTCA CTGATGTTAC TAAGAACAGT
    GTCACCTTGT CCTGGCAACC AGGTACCCCT GGAGCCCTTC CAGCAAGTGC ATATATCATT
    GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACGG TGGCAAACCA TGTAAAGACC
    ACGCTCTATA CAGTGAGAGG ATTGCGGCCC AATACGATCT ATTTATTCAT GGTCAGAGCT
    ATCAACCCCC AAGGTCTAAG CGACCCAAGC CCCATGTCAG ATCCTGTGCG CACACAAGAT
    ATCAGCCCAC CAGCACAAGG AGTGGACCAT AGACAAGTAC AGAAAGAACT AGGAGATGTC
    ATTGTCCGTC TTCATACTCC AGTTGTGTTG ACTCCCACCA CTGTTCAGGT CACATGGACG
    GTTGATCGCC AGCCCCAGTT TATTCAAGGC TACCGAGTGA TGTATCGTCA GACCACAGGC
    CTGCAGGCTA CGACAACATG GCAGAATTTA GATGCCAAAG TCCCAACCGA GCGAAGTGCT
    GTCTTGGTCA ACCTGAAAAA GGGAGTGACT TATGAAATTA AAGTTCGACC ATATTTTAAT
    GAGTTCCAAG GAATGGACAG TGAATCTAAA ATAGTTCGTA CTACTGAAGA AGCCCCAAGT
    GCCCCTCCAC AATCTGTCAC TGTGCTGACA GTTGGAAGCC ACAATAGCAC AAGTATTAGT
    GTTTCCTGGG ATCCTCCTCC TCCAGATCAT CAGAATGGAA TCATCCAAGA ATACAAGATC
    TGGTGTCTGG GGAATGAAAC ACGATTCCAT ATCAACAAAA CTGTGGATGC AGCCATTCGA
    TCTGTGATAA TTGGTGGATT GTTCCCAGGT ATTCAGTACC GGGTAGAGGT TGCAGCTAGT
    ACAAGTGCAG GGGTTGGAGT AAAAAGTGAG CCGCAGCCGA TAATAATTGG GGGACGTAAT
    GAAGTTGTCA TTACTGAAAA CAATAACAGC ATAACTGAGC AAATCACTGA TGTTGTGAAG
    CAACCAGCCT TTATAGCTGG AATTGGTGGT GCCTGCTGGG TAATTTTGAT GGGTTTTAGC
    ATCTGGTTGT ATTGGCGAAG AAAGAAGAGA AAGGGACTCA GTAACTATGC TGTTACATTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CTGGTCTTCT AAATGCTGGT
    GATCCCAGTT ACCCATGGCT TGCTGATTCA TGGCCAGCCA CGAGCTTACC AGTAAACAAT
    AGCAATAGTG GCCCAAATGA GATTGGGAAT TTTGGACGTG GAGATGTGCT GCCACCAGTT
    CCAGGCCAAG GGGATAAAAC AGCAACGATG CTCTCAGATG GAGCCATTTA TAGCAGCATT
    GACTTCACGA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC TACCCCATAT
    GCCACAACAC AGATCTTGCA TTCCAATAGC ATACATGAAT TGGCTGTCGA TCTGCCGGAC
    CCACAGTGGA AGAGCTCAAT TCAGCAAAAA ACGGACCTGA TGGGCTTTGG ATATTCTCTC
    CCTGATCAGA ACAAGGGTAA CAACGCCTTA CTTTACATCC CTGACTACCG ATTGGCTGAG
    GGATTGTCTA ATAGAATGCC ACACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC
    AACAGCTCAG AGAGGAGTGG CAGTCTCTCA GGTGGGAAAG GTGGAAAAAA GAAGAAAACT
    AAAAACTCTT CTAAACCACA GAAAAACAAT GGATCGACTT GGGCCAATGT ACCCCTACCT
    CCTCCCCCAG TCCAGCCCCT TCCTGGTACA GAGCTGGAAC ACTATGCAGT GGAACCGCAA
    GAAAATGGGT ATGACAGTGA TAGCTGGTGC CCGCCATTAC CGGTACAAAC ATACTTACAC
    CAGGGTATGG AAGATGAACT GGAAGAAGAT GATGATCGTG TCCCAACACC TCCTGTCCGA
    GGCGTGGCTT CTTCTCCTGC TATTTCCTTC GGACAGCAGT CCACTGCAAC TCTTACTCCA
    TCCCCACGGG AAGAGATGCA ACCCATGCTG CAAGCTCACC TGGATGAGTT GACAAGAGCC
    TATCAGTTTG ATATAGCGAA GCAAACATGG CACATTCAAA GCAATAATCA ACCTCCACAG
    CCCCCGGTTC CACCATTAGG TTACATGTCC GGAGCCTTGA TTTCCGATTT GGAAACAGAT
    GTTCCAGATG ATGACGCTGA TGATGAGGAG GAAGCTTTAG AAATCCCCAG ACCCCTGAGA
    GCACTAGAGC AGACACCCGG ATCCAGTATG GACAATCTAG ACAGCTCTGT GACAGGTTCA
    ATGGTAAATG GCTGGGGCTC TGCATCTGAT GAGGATCGTA ACTTTTCTAG TCATAGATCT
    AGTGTAGGTA GCTCCTCAGA TGGCTCCATC TTTGCCAGCG GCAGCTTTGC ACAAGCAGTG
    GTGGCAGCAG CAGATAAAGC TGGTTTTAGG CTGGATGGAA CCAGCCTTAC AAGAACAGGC
    AAAGCTTTTA CCTCCTCTCA AAGACCTCGA CCAACCAGCC CATTTTCTAC TGACAGTAAT
    ACCAGTGCAG CCCTGAGTCA AAATCAGAGG CCTCGGCCCA CTAAAAAACA CAAGGGAGGG
    CGAATGGATC AACAACCAGC ATTGCCTCAT CGAAGGGAAG GAATGACAGA TGATCTTCCA
    CCACCACCAG ATCCCCCTCC AGGTCAGGGT TTAAGGCAGC AAATAGGCCC GAGCCAGCAT
    GCTGGTAACA TGGAAAACTC AACAGAGAGA AAAGGAAGCT CTCTGGAGAC ACCACAAGCA
    TCCAACTTAG AAGACACAAA GAGCTCATTG GATTGTCCAG CTAGAACGTC CCTAGAGTGG
    CAGCGACAAA CCCAAGAATG GATAAACTCC ACAGAACGCC AAGAAGATAT ACGGAAAGCC
    CTACACAAAC AAAGTGTTGG ACCAGAGGAA ACATTGGTGC CATACAGCAA GCCCAGTTTC
    CCATCTCCGG GAGGTCACAG CTCATCAGGA ACAGCATCTT CTAAAGGATC CACTGGACCT
    AGGAAAGCCG AGGCGTTGAG GGGAAGTCAC CAACGCAATG CCAGTGACCT TCTTGACATA
    GGATATATGG GCTCAAATAG TCAAGGACAG TTTACAGGTG AATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268806.1
    CDS388..5175
    Translation

    Target ORF information:

    RefSeq Version XM_022413098.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413098.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCCTCC ACAGTTTGTG
    GTTAGGCCAA GAGATCAGAT TGTTGCTCAA GGTCGAACAG TTACATTTCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAAAAAGAAG GCAGCCAGAA CTTGCTTTTT
    CCAAATCAAC CCCAGCAACC CAACAGTAGA TGCTCTGTTT CGCCAACTGG AGACCTCACA
    ATTACCAACA TTCAGCGCTC CGATGCGGGT TACTATATCT GCCAGGCATT AACTGTGGCA
    GGAAGCATTT TAGCAAAAGC TCAACTGGAA GTTACTGATG TTTTAACAGA TAGACCTCCA
    CCTATAATTC TACAAGGCCC TGCAAACCAA ACTCTAGCAG TTGATGGTAC AGCATTACTG
    AAATGTAAAG CTACTGGGGA TCCTCTTCCT GTAATTAGCT GGTTAAAGGA GGGATTTACT
    TTTCTGGGTA GAGATTCAAG AGCAACTATA CAAGAACAAG GAACATTGCA GATTAAGAAT
    TTACGGATTT CTGATACTGG TACTTATACT TGTGTGGCTA CAAGTTCAAG TGGGGAGACT
    TCGTGGAGTG CTGTGCTGGA TGTGACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAC
    TTAAGTGACC TACCAGGGCC ACCATCCAAA CCACAGGTCA CTGATGTTAC TAAGAACAGT
    GTCACCTTGT CCTGGCAACC AGGTACCCCT GGAGCCCTTC CAGCAAGTGC ATATATCATT
    GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACGG TGGCAAACCA TGTAAAGACC
    ACGCTCTATA CAGTGAGAGG ATTGCGGCCC AATACGATCT ATTTATTCAT GGTCAGAGCT
    ATCAACCCCC AAGGTCTAAG CGACCCAAGC CCCATGTCAG ATCCTGTGCG CACACAAGAT
    ATCAGCCCAC CAGCACAAGG AGTGGACCAT AGACAAGTAC AGAAAGAACT AGGAGATGTC
    ATTGTCCGTC TTCATACTCC AGTTGTGTTG ACTCCCACCA CTGTTCAGGT CACATGGACG
    GTTGATCGCC AGCCCCAGTT TATTCAAGGC TACCGAGTGA TGTATCGTCA GACCACAGGC
    CTGCAGGCTA CGACAACATG GCAGAATTTA GATGCCAAAG TCCCAACCGA GCGAAGTGCT
    GTCTTGGTCA ACCTGAAAAA GGGAGTGACT TATGAAATTA AAGTTCGACC ATATTTTAAT
    GAGTTCCAAG GAATGGACAG TGAATCTAAA ATAGTTCGTA CTACTGAAGA AGCCCCAAGT
    GCCCCTCCAC AATCTGTCAC TGTGCTGACA GTTGGAAGCC ACAATAGCAC AAGTATTAGT
    GTTTCCTGGG ATCCTCCTCC TCCAGATCAT CAGAATGGAA TCATCCAAGA ATACAAGATC
    TGGTGTCTGG GGAATGAAAC ACGATTCCAT ATCAACAAAA CTGTGGATGC AGCCATTCGA
    TCTGTGATAA TTGGTGGATT GTTCCCAGGT ATTCAGTACC GGGTAGAGGT TGCAGCTAGT
    ACAAGTGCAG GGGTTGGAGT AAAAAGTGAG CCGCAGCCGA TAATAATTGG GGGACGTAAT
    GAAGTTGTCA TTACTGAAAA CAATAACAGC ATAACTGAGC AAATCACTGA TGTTGTGAAG
    CAACCAGCCT TTATAGCTGG AATTGGTGGT GCCTGCTGGG TAATTTTGAT GGGTTTTAGC
    ATCTGGTTGT ATTGGCGAAG AAAGAAGAGA AAGGGACTCA GTAACTATGC TGTTACATTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CTGGTCTTCT AAATGCTGGT
    GATCCCAGTT ACCCATGGCT TGCTGATTCA TGGCCAGCCA CGAGCTTACC AGTAAACAAT
    AGCAATAGTG GCCCAAATGA GATTGGGAAT TTTGGACGTG GAGATGTGCT GCCACCAGTT
    CCAGGCCAAG GGGATAAAAC AGCAACGATG CTCTCAGATG GAGCCATTTA TAGCAGCATT
    GACTTCACGA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC TACCCCATAT
    GCCACAACAC AGATCTTGCA TTCCAATAGC ATACATGAAT TGGCTGTCGA TCTGCCGGAC
    CCACAGTGGA AGAGCTCAAT TCAGCAAAAA ACGGACCTGA TGGGCTTTGG ATATTCTCTC
    CCTGATCAGA ACAAGGGTAA CAACGCCTTA CTTTACATCC CTGACTACCG ATTGGCTGAG
    GGATTGTCTA ATAGAATGCC ACACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC
    AACAGCTCAG AGAGGAGTGG CAGTCTCTCA GGTGGGAAAG GTGGAAAAAA GAAGAAAACT
    AAAAACTCTT CTAAACCACA GAAAAACAAT GGATCGACTT GGGCCAATGT ACCCCTACCT
    CCTCCCCCAG TCCAGCCCCT TCCTGGTACA GAGCTGGAAC ACTATGCAGT GGAACCGCAA
    GAAAATGGGT ATGACAGTGA TAGCTGGTGC CCGCCATTAC CGGTACAAAC ATACTTACAC
    CAGGGTATGG AAGATGAACT GGAAGAAGAT GATGATCGTG TCCCAACACC TCCTGTCCGA
    GGCGTGGCTT CTTCTCCTGC TATTTCCTTC GGACAGCAGT CCACTGCAAC TCTTACTCCA
    TCCCCACGGG AAGAGATGCA ACCCATGCTG CAAGCTCACC TGGATGAGTT GACAAGAGCC
    TATCAGTTTG ATATAGCGAA GCAAACATGG CACATTCAAA GCAATAATCA ACCTCCACAG
    CCCCCGGTTC CACCATTAGG TTACATGTCC GGAGCCTTGA TTTCCGATTT GGAAACAGAT
    GTTCCAGATG ATGACGCTGA TGATGAGGAG GAAGCTTTAG AAATCCCCAG ACCCCTGAGA
    GCACTAGAGC AGACACCCGG ATCCAGTATG GACAATCTAG ACAGCTCTGT GACAGGTTCA
    ATGGTAAATG GCTGGGGCTC TGCATCTGAT GAGGATCGTA ACTTTTCTAG TCATAGATCT
    AGTGTAGGTA GCTCCTCAGA TGGCTCCATC TTTGCCAGCG GCAGCTTTGC ACAAGCAGTG
    GTGGCAGCAG CAGATAAAGC TGGTTTTAGG CTGGATGGAA CCAGCCTTAC AAGAACAGGC
    AAAGCTTTTA CCTCCTCTCA AAGACCTCGA CCAACCAGCC CATTTTCTAC TGACAGTAAT
    ACCAGTGCAG CCCTGAGTCA AAATCAGAGG CCTCGGCCCA CTAAAAAACA CAAGGGAGGG
    CGAATGGATC AACAACCAGC ATTGCCTCAT CGAAGGGAAG GAATGACAGA TGATCTTCCA
    CCACCACCAG ATCCCCCTCC AGGTCAGGGT TTAAGGCAGC AAATAGGCCC GAGCCAGCAT
    GCTGGTAACA TGGAAAACTC AACAGAGAGA AAAGGAAGCT CTCTGGAGAC ACCACAAGCA
    TCCAACTTAG AAGACACAAA GAGCTCATTG GATTGTCCAG CTAGAACGTC CCTAGAGTGG
    CAGCGACAAA CCCAAGAATG GATAAACTCC ACAGAACGCC AAGAAGATAT ACGGAAAGCC
    CTACACAAAC AAAGTGTTGG ACCAGAGGAA ACATTGGTGC CATACAGCAA GCCCAGTTTC
    CCATCTCCGG GAGGTCACAG CTCATCAGGA ACAGCATCTT CTAAAGGATC CACTGGACCT
    AGGAAAGCCG AGGCGTTGAG GGGAAGTCAC CAACGCAATG CCAGTGACCT TCTTGACATA
    GGATATATGG GCTCAAATAG TCAAGGACAG TTTACAGGTG AATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe283875
    Clone ID Related Accession (Same CDS sequence) XM_022413099.1
    Accession Version XM_022413099.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4779bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product roundabout homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACCAGAA GACAAGGAAG TGTTACTAGA AGAATGTGGA CATGGACTCC GGAACTGCTA 
    ATGGTGACTG TGATCCTCTG GGGTCAGCAA GGAAATGGAC AAGGCCAAGG ATCTCGTCTT
    CGCCAAGAAG ACTTCCCTCC GCGGATTGTG GAGCACCCTT CAGATGTCAT TGTCTCTAAG
    GGAGAGCCCA CCACCTTGAA CTGTAAGGCA GAGGGCCGGC CAACGCCCAC CATTGAGTGG
    TACAAGGATG GCGAGCGGGT GGAGACTGAC AAGGATGATC CCCGGTCTCA CAGGATGCTT
    CTGCCCAGCG GATCCTTATT TTTCTTGCGC ATTGTTCATG GGCGCAGAAG CAAACCCGAT
    GAAGGAAGCT ACGTCTGTGT TGCGAGGAAC TATCTCGGTG AAGCAGTAAG TCGAAATGCA
    TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGGCAAA ATCCCACAGA TGTTGTAGTG
    GCAGCCGGAG AGCCTGCAAT CCTGGAGTGC CAACCTCCCA GGGGACACCC AGAACCCACG
    ATCTACTGGA AAAAAGACAA AGTCCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT
    GGTGGAAAGC TGATGATATC CAATACCAGG AAAAGTGATG CAGGGATGTA TACCTGTGTT
    GGCACCAATA TGGTGGGAGA AAGAGACAGT GATCCAGCAG AGCTGACTGT CTTTGAACGA
    CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AAGAAGAAGC TGTAGAATTT
    CGTTGTCAGG TCCAAGGAGA TCCTCAACCA ACTGTGCGGT GGAAAAAGGA TGATGCAGAC
    CTGCCAAGAG GAAGGTATGA CATCAAAGAT GATTACACAC TGAGAATTAA AAAGGCCATG
    AGTACAGATG AAGGCACCTA TCTGTGTATT GCCGAGAATC GGGTTGGAAA AGTAGAAGCC
    TCTGCTTCTC TAACAGTCCG AGCTCGCCCT GTTGCTCCTC CACAGTTTGT GGTTAGGCCA
    AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTTACATTTC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAAAAAGAA GGCAGCCAGA ACTTGCTTTT TCCAAATCAA
    CCCCAGCAAC CCAACAGTAG ATGCTCTGTT TCGCCAACTG GAGACCTCAC AATTACCAAC
    ATTCAGCGCT CCGATGCGGG TTACTATATC TGCCAGGCAT TAACTGTGGC AGGAAGCATT
    TTAGCAAAAG CTCAACTGGA AGTTACTGAT GTTTTAACAG ATAGACCTCC ACCTATAATT
    CTACAAGGCC CTGCAAACCA AACTCTAGCA GTTGATGGTA CAGCATTACT GAAATGTAAA
    GCTACTGGGG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCTGGGT
    AGAGATTCAA GAGCAACTAT ACAAGAACAA GGAACATTGC AGATTAAGAA TTTACGGATT
    TCTGATACTG GTACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGGGAGAC TTCGTGGAGT
    GCTGTGCTGG ATGTGACAGA ATCTGGAGCA ACAATCAGTA AAAATTATGA CTTAAGTGAC
    CTACCAGGGC CACCATCCAA ACCACAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG
    TCCTGGCAAC CAGGTACCCC TGGAGCCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC
    AGCCAATCGG TGAGCAATAG CTGGCAGACG GTGGCAAACC ATGTAAAGAC CACGCTCTAT
    ACAGTGAGAG GATTGCGGCC CAATACGATC TATTTATTCA TGGTCAGAGC TATCAACCCC
    CAAGGTCTAA GCGACCCAAG CCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA
    CCAGCACAAG GAGTGGACCA TAGACAAGTA CAGAAAGAAC TAGGAGATGT CATTGTCCGT
    CTTCATACTC CAGTTGTGTT GACTCCCACC ACTGTTCAGG TCACATGGAC GGTTGATCGC
    CAGCCCCAGT TTATTCAAGG CTACCGAGTG ATGTATCGTC AGACCACAGG CCTGCAGGCT
    ACGACAACAT GGCAGAATTT AGATGCCAAA GTCCCAACCG AGCGAAGTGC TGTCTTGGTC
    AACCTGAAAA AGGGAGTGAC TTATGAAATT AAAGTTCGAC CATATTTTAA TGAGTTCCAA
    GGAATGGACA GTGAATCTAA AATAGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCTCCA
    CAATCTGTCA CTGTGCTGAC AGTTGGAAGC CACAATAGCA CAAGTATTAG TGTTTCCTGG
    GATCCTCCTC CTCCAGATCA TCAGAATGGA ATCATCCAAG AATACAAGAT CTGGTGTCTG
    GGGAATGAAA CACGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG ATCTGTGATA
    ATTGGTGGAT TGTTCCCAGG TATTCAGTAC CGGGTAGAGG TTGCAGCTAG TACAAGTGCA
    GGGGTTGGAG TAAAAAGTGA GCCGCAGCCG ATAATAATTG GGGGACGTAA TGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTTGTGAA GCAACCAGCC
    TTTATAGCTG GAATTGGTGG TGCCTGCTGG GTAATTTTGA TGGGTTTTAG CATCTGGTTG
    TATTGGCGAA GAAAGAAGAG AAAGGGACTC AGTAACTATG CTGTTACATT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGGAGCCGT CCTGGTCTTC TAAATGCTGG TGATCCCAGT
    TACCCATGGC TTGCTGATTC ATGGCCAGCC ACGAGCTTAC CAGTAAACAA TAGCAATAGT
    GGCCCAAATG AGATTGGGAA TTTTGGACGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA
    GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGCAGCAT TGACTTCACG
    ACCAAAACCA CTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACAACA
    CAGATCTTGC ATTCCAATAG CATACATGAA TTGGCTGTCG ATCTGCCGGA CCCACAGTGG
    AAGAGCTCAA TTCAGCAAAA AACGGACCTG ATGGGCTTTG GATATTCTCT CCCTGATCAG
    AACAAGGGTA ACAACGCCTT ACTTTACATC CCTGACTACC GATTGGCTGA GGGATTGTCT
    AATAGAATGC CACACAACCA GTCACAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA
    GAGAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAC TAAAAACTCT
    TCTAAACCAC AGAAAAACAA TGGATCGACT TGGGCCAATG TACCCCTACC TCCTCCCCCA
    GTCCAGCCCC TTCCTGGTAC AGAGCTGGAA CACTATGCAG TGGAACCGCA AGAAAATGGG
    TATGACAGTG ATAGCTGGTG CCCGCCATTA CCGGTACAAA CATACTTACA CCAGGGTATG
    GAAGATGAAC TGGAAGAAGA TGATGATCGT GTCCCAACAC CTCCTGTCCG AGGCGTGGCT
    TCTTCTCCTG CTATTTCCTT CGGACAGCAG TCCACTGCAA CTCTTACTCC ATCCCCACGG
    GAAGAGATGC AACCCATGCT GCAAGCTCAC CTGGATGAGT TGACAAGAGC CTATCAGTTT
    GATATAGCGA AGCAAACATG GCACATTCAA AGCAATAATC AACCTCCACA GCCCCCGGTT
    CCACCATTAG GTTACATGTC CGGAGCCTTG ATTTCCGATT TGGAAACAGA TGTTCCAGAT
    GATGACGCTG ATGATGAGGA GGAAGCTTTA GAAATCCCCA GACCCCTGAG AGCACTAGAG
    CAGACACCCG GATCCAGTAT GGACAATCTA GACAGCTCTG TGACAGGTTC AATGGTAAAT
    GGCTGGGGCT CTGCATCTGA TGAGGATCGT AACTTTTCTA GTCATAGATC TAGTGTAGGT
    AGCTCCTCAG ATGGCTCCAT CTTTGCCAGC GGCAGCTTTG CACAAGCAGT GGTGGCAGCA
    GCAGATAAAG CTGGTTTTAG GCTGGATGGA ACCAGCCTTA CAAGAACAGG CAAAGCTTTT
    ACCTCCTCTC AAAGACCTCG ACCAACCAGC CCATTTTCTA CTGACAGTAA TACCAGTGCA
    GCCCTGAGTC AAAATCAGAG GCCTCGGCCC ACTAAAAAAC ACAAGGGAGG GCGAATGGAT
    CAACAACCAG CATTGCCTCA TCGAAGGGAA GGAATGACAG ATGATCTTCC ACCACCACCA
    GATCCCCCTC CAGGTCAGGG TTTAAGGCAG CAAATAGGCC CGAGCCAGCA TGCTGGTAAC
    ATGGAAAACT CAACAGAGAG AAAAGGAAGC TCTCTGGAGA CACCACAAGC ATCCAACTTA
    GAAGACACAA AGAGCTCATT GGATTGTCCA GCTAGAACGT CCCTAGAGTG GCAGCGACAA
    ACCCAAGAAT GGATAAACTC CACAGAACGC CAAGAAGATA TACGGAAAGC CCTACACAAA
    CAAAGTGTTG GACCAGAGGA AACATTGGTG CCATACAGCA AGCCCAGTTT CCCATCTCCG
    GGAGGTCACA GCTCATCAGG AACAGCATCT TCTAAAGGAT CCACTGGACC TAGGAAAGCC
    GAGGCGTTGA GGGGAAGTCA CCAACGCAAT GCCAGTGACC TTCTTGACAT AGGATATATG
    GGCTCAAATA GTCAAGGACA GTTTACAGGT GAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268807.1
    CDS164..4942
    Translation

    Target ORF information:

    RefSeq Version XM_022413099.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413099.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACCAGAA GACAAGGAAG TGTTACTAGA AGAATGTGGA CATGGACTCC GGAACTGCTA 
    ATGGTGACTG TGATCCTCTG GGGTCAGCAA GGAAATGGAC AAGGCCAAGG ATCTCGTCTT
    CGCCAAGAAG ACTTCCCTCC GCGGATTGTG GAGCACCCTT CAGATGTCAT TGTCTCTAAG
    GGAGAGCCCA CCACCTTGAA CTGTAAGGCA GAGGGCCGGC CAACGCCCAC CATTGAGTGG
    TACAAGGATG GCGAGCGGGT GGAGACTGAC AAGGATGATC CCCGGTCTCA CAGGATGCTT
    CTGCCCAGCG GATCCTTATT TTTCTTGCGC ATTGTTCATG GGCGCAGAAG CAAACCCGAT
    GAAGGAAGCT ACGTCTGTGT TGCGAGGAAC TATCTCGGTG AAGCAGTAAG TCGAAATGCA
    TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGGCAAA ATCCCACAGA TGTTGTAGTG
    GCAGCCGGAG AGCCTGCAAT CCTGGAGTGC CAACCTCCCA GGGGACACCC AGAACCCACG
    ATCTACTGGA AAAAAGACAA AGTCCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT
    GGTGGAAAGC TGATGATATC CAATACCAGG AAAAGTGATG CAGGGATGTA TACCTGTGTT
    GGCACCAATA TGGTGGGAGA AAGAGACAGT GATCCAGCAG AGCTGACTGT CTTTGAACGA
    CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AAGAAGAAGC TGTAGAATTT
    CGTTGTCAGG TCCAAGGAGA TCCTCAACCA ACTGTGCGGT GGAAAAAGGA TGATGCAGAC
    CTGCCAAGAG GAAGGTATGA CATCAAAGAT GATTACACAC TGAGAATTAA AAAGGCCATG
    AGTACAGATG AAGGCACCTA TCTGTGTATT GCCGAGAATC GGGTTGGAAA AGTAGAAGCC
    TCTGCTTCTC TAACAGTCCG AGCTCGCCCT GTTGCTCCTC CACAGTTTGT GGTTAGGCCA
    AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTTACATTTC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAAAAAGAA GGCAGCCAGA ACTTGCTTTT TCCAAATCAA
    CCCCAGCAAC CCAACAGTAG ATGCTCTGTT TCGCCAACTG GAGACCTCAC AATTACCAAC
    ATTCAGCGCT CCGATGCGGG TTACTATATC TGCCAGGCAT TAACTGTGGC AGGAAGCATT
    TTAGCAAAAG CTCAACTGGA AGTTACTGAT GTTTTAACAG ATAGACCTCC ACCTATAATT
    CTACAAGGCC CTGCAAACCA AACTCTAGCA GTTGATGGTA CAGCATTACT GAAATGTAAA
    GCTACTGGGG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCTGGGT
    AGAGATTCAA GAGCAACTAT ACAAGAACAA GGAACATTGC AGATTAAGAA TTTACGGATT
    TCTGATACTG GTACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGGGAGAC TTCGTGGAGT
    GCTGTGCTGG ATGTGACAGA ATCTGGAGCA ACAATCAGTA AAAATTATGA CTTAAGTGAC
    CTACCAGGGC CACCATCCAA ACCACAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG
    TCCTGGCAAC CAGGTACCCC TGGAGCCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC
    AGCCAATCGG TGAGCAATAG CTGGCAGACG GTGGCAAACC ATGTAAAGAC CACGCTCTAT
    ACAGTGAGAG GATTGCGGCC CAATACGATC TATTTATTCA TGGTCAGAGC TATCAACCCC
    CAAGGTCTAA GCGACCCAAG CCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA
    CCAGCACAAG GAGTGGACCA TAGACAAGTA CAGAAAGAAC TAGGAGATGT CATTGTCCGT
    CTTCATACTC CAGTTGTGTT GACTCCCACC ACTGTTCAGG TCACATGGAC GGTTGATCGC
    CAGCCCCAGT TTATTCAAGG CTACCGAGTG ATGTATCGTC AGACCACAGG CCTGCAGGCT
    ACGACAACAT GGCAGAATTT AGATGCCAAA GTCCCAACCG AGCGAAGTGC TGTCTTGGTC
    AACCTGAAAA AGGGAGTGAC TTATGAAATT AAAGTTCGAC CATATTTTAA TGAGTTCCAA
    GGAATGGACA GTGAATCTAA AATAGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCTCCA
    CAATCTGTCA CTGTGCTGAC AGTTGGAAGC CACAATAGCA CAAGTATTAG TGTTTCCTGG
    GATCCTCCTC CTCCAGATCA TCAGAATGGA ATCATCCAAG AATACAAGAT CTGGTGTCTG
    GGGAATGAAA CACGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG ATCTGTGATA
    ATTGGTGGAT TGTTCCCAGG TATTCAGTAC CGGGTAGAGG TTGCAGCTAG TACAAGTGCA
    GGGGTTGGAG TAAAAAGTGA GCCGCAGCCG ATAATAATTG GGGGACGTAA TGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTTGTGAA GCAACCAGCC
    TTTATAGCTG GAATTGGTGG TGCCTGCTGG GTAATTTTGA TGGGTTTTAG CATCTGGTTG
    TATTGGCGAA GAAAGAAGAG AAAGGGACTC AGTAACTATG CTGTTACATT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGGAGCCGT CCTGGTCTTC TAAATGCTGG TGATCCCAGT
    TACCCATGGC TTGCTGATTC ATGGCCAGCC ACGAGCTTAC CAGTAAACAA TAGCAATAGT
    GGCCCAAATG AGATTGGGAA TTTTGGACGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA
    GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGCAGCAT TGACTTCACG
    ACCAAAACCA CTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACAACA
    CAGATCTTGC ATTCCAATAG CATACATGAA TTGGCTGTCG ATCTGCCGGA CCCACAGTGG
    AAGAGCTCAA TTCAGCAAAA AACGGACCTG ATGGGCTTTG GATATTCTCT CCCTGATCAG
    AACAAGGGTA ACAACGCCTT ACTTTACATC CCTGACTACC GATTGGCTGA GGGATTGTCT
    AATAGAATGC CACACAACCA GTCACAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA
    GAGAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAC TAAAAACTCT
    TCTAAACCAC AGAAAAACAA TGGATCGACT TGGGCCAATG TACCCCTACC TCCTCCCCCA
    GTCCAGCCCC TTCCTGGTAC AGAGCTGGAA CACTATGCAG TGGAACCGCA AGAAAATGGG
    TATGACAGTG ATAGCTGGTG CCCGCCATTA CCGGTACAAA CATACTTACA CCAGGGTATG
    GAAGATGAAC TGGAAGAAGA TGATGATCGT GTCCCAACAC CTCCTGTCCG AGGCGTGGCT
    TCTTCTCCTG CTATTTCCTT CGGACAGCAG TCCACTGCAA CTCTTACTCC ATCCCCACGG
    GAAGAGATGC AACCCATGCT GCAAGCTCAC CTGGATGAGT TGACAAGAGC CTATCAGTTT
    GATATAGCGA AGCAAACATG GCACATTCAA AGCAATAATC AACCTCCACA GCCCCCGGTT
    CCACCATTAG GTTACATGTC CGGAGCCTTG ATTTCCGATT TGGAAACAGA TGTTCCAGAT
    GATGACGCTG ATGATGAGGA GGAAGCTTTA GAAATCCCCA GACCCCTGAG AGCACTAGAG
    CAGACACCCG GATCCAGTAT GGACAATCTA GACAGCTCTG TGACAGGTTC AATGGTAAAT
    GGCTGGGGCT CTGCATCTGA TGAGGATCGT AACTTTTCTA GTCATAGATC TAGTGTAGGT
    AGCTCCTCAG ATGGCTCCAT CTTTGCCAGC GGCAGCTTTG CACAAGCAGT GGTGGCAGCA
    GCAGATAAAG CTGGTTTTAG GCTGGATGGA ACCAGCCTTA CAAGAACAGG CAAAGCTTTT
    ACCTCCTCTC AAAGACCTCG ACCAACCAGC CCATTTTCTA CTGACAGTAA TACCAGTGCA
    GCCCTGAGTC AAAATCAGAG GCCTCGGCCC ACTAAAAAAC ACAAGGGAGG GCGAATGGAT
    CAACAACCAG CATTGCCTCA TCGAAGGGAA GGAATGACAG ATGATCTTCC ACCACCACCA
    GATCCCCCTC CAGGTCAGGG TTTAAGGCAG CAAATAGGCC CGAGCCAGCA TGCTGGTAAC
    ATGGAAAACT CAACAGAGAG AAAAGGAAGC TCTCTGGAGA CACCACAAGC ATCCAACTTA
    GAAGACACAA AGAGCTCATT GGATTGTCCA GCTAGAACGT CCCTAGAGTG GCAGCGACAA
    ACCCAAGAAT GGATAAACTC CACAGAACGC CAAGAAGATA TACGGAAAGC CCTACACAAA
    CAAAGTGTTG GACCAGAGGA AACATTGGTG CCATACAGCA AGCCCAGTTT CCCATCTCCG
    GGAGGTCACA GCTCATCAGG AACAGCATCT TCTAAAGGAT CCACTGGACC TAGGAAAGCC
    GAGGCGTTGA GGGGAAGTCA CCAACGCAAT GCCAGTGACC TTCTTGACAT AGGATATATG
    GGCTCAAATA GTCAAGGACA GTTTACAGGT GAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe283876
    Clone ID Related Accession (Same CDS sequence) XM_022413100.1
    Accession Version XM_022413100.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product roundabout homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAGTCGGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TGTTATATGT TCCGGTTGAT 
    GGATCTCGTC TTCGCCAAGA AGACTTCCCT CCGCGGATTG TGGAGCACCC TTCAGATGTC
    ATTGTCTCTA AGGGAGAGCC CACCACCTTG AACTGTAAGG CAGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAGGA TGGCGAGCGG GTGGAGACTG ACAAGGATGA TCCCCGGTCT
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTTTTCTTGC GCATTGTTCA TGGGCGCAGA
    AGCAAACCCG ATGAAGGAAG CTACGTCTGT GTTGCGAGGA ACTATCTCGG TGAAGCAGTA
    AGTCGAAATG CATCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGGCA AAATCCCACA
    GATGTTGTAG TGGCAGCCGG AGAGCCTGCA ATCCTGGAGT GCCAACCTCC CAGGGGACAC
    CCAGAACCCA CGATCTACTG GAAAAAAGAC AAAGTCCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA GCTGATGATA TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACCTGTG TTGGCACCAA TATGGTGGGA GAAAGAGACA GTGATCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAAGAAGAA
    GCTGTAGAAT TTCGTTGTCA GGTCCAAGGA GATCCTCAAC CAACTGTGCG GTGGAAAAAG
    GATGATGCAG ACCTGCCAAG AGGAAGGTAT GACATCAAAG ATGATTACAC ACTGAGAATT
    AAAAAGGCCA TGAGTACAGA TGAAGGCACC TATCTGTGTA TTGCCGAGAA TCGGGTTGGA
    AAAGTAGAAG CCTCTGCTTC TCTAACAGTC CGAGCTCGCC CTGTTGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTTACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAAAAAG AAGGCAGCCA GAACTTGCTT
    TTTCCAAATC AACCCCAGCA ACCCAACAGT AGATGCTCTG TTTCGCCAAC TGGAGACCTC
    ACAATTACCA ACATTCAGCG CTCCGATGCG GGTTACTATA TCTGCCAGGC ATTAACTGTG
    GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAAGTTACTG ATGTTTTAAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCTGCAAAC CAAACTCTAG CAGTTGATGG TACAGCATTA
    CTGAAATGTA AAGCTACTGG GGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCTGG GTAGAGATTC AAGAGCAACT ATACAAGAAC AAGGAACATT GCAGATTAAG
    AATTTACGGA TTTCTGATAC TGGTACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGGGAG
    ACTTCGTGGA GTGCTGTGCT GGATGTGACA GAATCTGGAG CAACAATCAG TAAAAATTAT
    GACTTAAGTG ACCTACCAGG GCCACCATCC AAACCACAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA ACCAGGTACC CCTGGAGCCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CGGTGGCAAA CCATGTAAAG
    ACCACGCTCT ATACAGTGAG AGGATTGCGG CCCAATACGA TCTATTTATT CATGGTCAGA
    GCTATCAACC CCCAAGGTCT AAGCGACCCA AGCCCCATGT CAGATCCTGT GCGCACACAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CATAGACAAG TACAGAAAGA ACTAGGAGAT
    GTCATTGTCC GTCTTCATAC TCCAGTTGTG TTGACTCCCA CCACTGTTCA GGTCACATGG
    ACGGTTGATC GCCAGCCCCA GTTTATTCAA GGCTACCGAG TGATGTATCG TCAGACCACA
    GGCCTGCAGG CTACGACAAC ATGGCAGAAT TTAGATGCCA AAGTCCCAAC CGAGCGAAGT
    GCTGTCTTGG TCAACCTGAA AAAGGGAGTG ACTTATGAAA TTAAAGTTCG ACCATATTTT
    AATGAGTTCC AAGGAATGGA CAGTGAATCT AAAATAGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCTC CACAATCTGT CACTGTGCTG ACAGTTGGAA GCCACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CATCAGAATG GAATCATCCA AGAATACAAG
    ATCTGGTGTC TGGGGAATGA AACACGATTC CATATCAACA AAACTGTGGA TGCAGCCATT
    CGATCTGTGA TAATTGGTGG ATTGTTCCCA GGTATTCAGT ACCGGGTAGA GGTTGCAGCT
    AGTACAAGTG CAGGGGTTGG AGTAAAAAGT GAGCCGCAGC CGATAATAAT TGGGGGACGT
    AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTTGTG
    AAGCAACCAG CCTTTATAGC TGGAATTGGT GGTGCCTGCT GGGTAATTTT GATGGGTTTT
    AGCATCTGGT TGTATTGGCG AAGAAAGAAG AGAAAGGGAC TCAGTAACTA TGCTGTTACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGGAGCC GTCCTGGTCT TCTAAATGCT
    GGTGATCCCA GTTACCCATG GCTTGCTGAT TCATGGCCAG CCACGAGCTT ACCAGTAAAC
    AATAGCAATA GTGGCCCAAA TGAGATTGGG AATTTTGGAC GTGGAGATGT GCTGCCACCA
    GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CGACCAAAAC CACTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACAA CACAGATCTT GCATTCCAAT AGCATACATG AATTGGCTGT CGATCTGCCG
    GACCCACAGT GGAAGAGCTC AATTCAGCAA AAAACGGACC TGATGGGCTT TGGATATTCT
    CTCCCTGATC AGAACAAGGG TAACAACGCC TTACTTTACA TCCCTGACTA CCGATTGGCT
    GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCACAGG ATTTCAGCAC CACCAGCTCT
    CACAACAGCT CAGAGAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA
    ACTAAAAACT CTTCTAAACC ACAGAAAAAC AATGGATCGA CTTGGGCCAA TGTACCCCTA
    CCTCCTCCCC CAGTCCAGCC CCTTCCTGGT ACAGAGCTGG AACACTATGC AGTGGAACCG
    CAAGAAAATG GGTATGACAG TGATAGCTGG TGCCCGCCAT TACCGGTACA AACATACTTA
    CACCAGGGTA TGGAAGATGA ACTGGAAGAA GATGATGATC GTGTCCCAAC ACCTCCTGTC
    CGAGGCGTGG CTTCTTCTCC TGCTATTTCC TTCGGACAGC AGTCCACTGC AACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACCTGGATGA GTTGACAAGA
    GCCTATCAGT TTGATATAGC GAAGCAAACA TGGCACATTC AAAGCAATAA TCAACCTCCA
    CAGCCCCCGG TTCCACCATT AGGTTACATG TCCGGAGCCT TGATTTCCGA TTTGGAAACA
    GATGTTCCAG ATGATGACGC TGATGATGAG GAGGAAGCTT TAGAAATCCC CAGACCCCTG
    AGAGCACTAG AGCAGACACC CGGATCCAGT ATGGACAATC TAGACAGCTC TGTGACAGGT
    TCAATGGTAA ATGGCTGGGG CTCTGCATCT GATGAGGATC GTAACTTTTC TAGTCATAGA
    TCTAGTGTAG GTAGCTCCTC AGATGGCTCC ATCTTTGCCA GCGGCAGCTT TGCACAAGCA
    GTGGTGGCAG CAGCAGATAA AGCTGGTTTT AGGCTGGATG GAACCAGCCT TACAAGAACA
    GGCAAAGCTT TTACCTCCTC TCAAAGACCT CGACCAACCA GCCCATTTTC TACTGACAGT
    AATACCAGTG CAGCCCTGAG TCAAAATCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA
    GGGCGAATGG ATCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGATCTT
    CCACCACCAC CAGATCCCCC TCCAGGTCAG GGTTTAAGGC AGCAAATAGG CCCGAGCCAG
    CATGCTGGTA ACATGGAAAA CTCAACAGAG AGAAAAGGAA GCTCTCTGGA GACACCACAA
    GCATCCAACT TAGAAGACAC AAAGAGCTCA TTGGATTGTC CAGCTAGAAC GTCCCTAGAG
    TGGCAGCGAC AAACCCAAGA ATGGATAAAC TCCACAGAAC GCCAAGAAGA TATACGGAAA
    GCCCTACACA AACAAAGTGT TGGACCAGAG GAAACATTGG TGCCATACAG CAAGCCCAGT
    TTCCCATCTC CGGGAGGTCA CAGCTCATCA GGAACAGCAT CTTCTAAAGG ATCCACTGGA
    CCTAGGAAAG CCGAGGCGTT GAGGGGAAGT CACCAACGCA ATGCCAGTGA CCTTCTTGAC
    ATAGGATATA TGGGCTCAAA TAGTCAAGGA CAGTTTACAG GTGAATTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268808.1
    CDS799..5529
    Translation

    Target ORF information:

    RefSeq Version XM_022413100.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413100.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAGTCGGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TGTTATATGT TCCGGTTGAT 
    GGATCTCGTC TTCGCCAAGA AGACTTCCCT CCGCGGATTG TGGAGCACCC TTCAGATGTC
    ATTGTCTCTA AGGGAGAGCC CACCACCTTG AACTGTAAGG CAGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAGGA TGGCGAGCGG GTGGAGACTG ACAAGGATGA TCCCCGGTCT
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTTTTCTTGC GCATTGTTCA TGGGCGCAGA
    AGCAAACCCG ATGAAGGAAG CTACGTCTGT GTTGCGAGGA ACTATCTCGG TGAAGCAGTA
    AGTCGAAATG CATCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGGCA AAATCCCACA
    GATGTTGTAG TGGCAGCCGG AGAGCCTGCA ATCCTGGAGT GCCAACCTCC CAGGGGACAC
    CCAGAACCCA CGATCTACTG GAAAAAAGAC AAAGTCCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA GCTGATGATA TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACCTGTG TTGGCACCAA TATGGTGGGA GAAAGAGACA GTGATCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAAGAAGAA
    GCTGTAGAAT TTCGTTGTCA GGTCCAAGGA GATCCTCAAC CAACTGTGCG GTGGAAAAAG
    GATGATGCAG ACCTGCCAAG AGGAAGGTAT GACATCAAAG ATGATTACAC ACTGAGAATT
    AAAAAGGCCA TGAGTACAGA TGAAGGCACC TATCTGTGTA TTGCCGAGAA TCGGGTTGGA
    AAAGTAGAAG CCTCTGCTTC TCTAACAGTC CGAGCTCGCC CTGTTGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTTACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAAAAAG AAGGCAGCCA GAACTTGCTT
    TTTCCAAATC AACCCCAGCA ACCCAACAGT AGATGCTCTG TTTCGCCAAC TGGAGACCTC
    ACAATTACCA ACATTCAGCG CTCCGATGCG GGTTACTATA TCTGCCAGGC ATTAACTGTG
    GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAAGTTACTG ATGTTTTAAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCTGCAAAC CAAACTCTAG CAGTTGATGG TACAGCATTA
    CTGAAATGTA AAGCTACTGG GGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCTGG GTAGAGATTC AAGAGCAACT ATACAAGAAC AAGGAACATT GCAGATTAAG
    AATTTACGGA TTTCTGATAC TGGTACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGGGAG
    ACTTCGTGGA GTGCTGTGCT GGATGTGACA GAATCTGGAG CAACAATCAG TAAAAATTAT
    GACTTAAGTG ACCTACCAGG GCCACCATCC AAACCACAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA ACCAGGTACC CCTGGAGCCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CGGTGGCAAA CCATGTAAAG
    ACCACGCTCT ATACAGTGAG AGGATTGCGG CCCAATACGA TCTATTTATT CATGGTCAGA
    GCTATCAACC CCCAAGGTCT AAGCGACCCA AGCCCCATGT CAGATCCTGT GCGCACACAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CATAGACAAG TACAGAAAGA ACTAGGAGAT
    GTCATTGTCC GTCTTCATAC TCCAGTTGTG TTGACTCCCA CCACTGTTCA GGTCACATGG
    ACGGTTGATC GCCAGCCCCA GTTTATTCAA GGCTACCGAG TGATGTATCG TCAGACCACA
    GGCCTGCAGG CTACGACAAC ATGGCAGAAT TTAGATGCCA AAGTCCCAAC CGAGCGAAGT
    GCTGTCTTGG TCAACCTGAA AAAGGGAGTG ACTTATGAAA TTAAAGTTCG ACCATATTTT
    AATGAGTTCC AAGGAATGGA CAGTGAATCT AAAATAGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCTC CACAATCTGT CACTGTGCTG ACAGTTGGAA GCCACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CATCAGAATG GAATCATCCA AGAATACAAG
    ATCTGGTGTC TGGGGAATGA AACACGATTC CATATCAACA AAACTGTGGA TGCAGCCATT
    CGATCTGTGA TAATTGGTGG ATTGTTCCCA GGTATTCAGT ACCGGGTAGA GGTTGCAGCT
    AGTACAAGTG CAGGGGTTGG AGTAAAAAGT GAGCCGCAGC CGATAATAAT TGGGGGACGT
    AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTTGTG
    AAGCAACCAG CCTTTATAGC TGGAATTGGT GGTGCCTGCT GGGTAATTTT GATGGGTTTT
    AGCATCTGGT TGTATTGGCG AAGAAAGAAG AGAAAGGGAC TCAGTAACTA TGCTGTTACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGGAGCC GTCCTGGTCT TCTAAATGCT
    GGTGATCCCA GTTACCCATG GCTTGCTGAT TCATGGCCAG CCACGAGCTT ACCAGTAAAC
    AATAGCAATA GTGGCCCAAA TGAGATTGGG AATTTTGGAC GTGGAGATGT GCTGCCACCA
    GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CGACCAAAAC CACTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACAA CACAGATCTT GCATTCCAAT AGCATACATG AATTGGCTGT CGATCTGCCG
    GACCCACAGT GGAAGAGCTC AATTCAGCAA AAAACGGACC TGATGGGCTT TGGATATTCT
    CTCCCTGATC AGAACAAGGG TAACAACGCC TTACTTTACA TCCCTGACTA CCGATTGGCT
    GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCACAGG ATTTCAGCAC CACCAGCTCT
    CACAACAGCT CAGAGAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA
    ACTAAAAACT CTTCTAAACC ACAGAAAAAC AATGGATCGA CTTGGGCCAA TGTACCCCTA
    CCTCCTCCCC CAGTCCAGCC CCTTCCTGGT ACAGAGCTGG AACACTATGC AGTGGAACCG
    CAAGAAAATG GGTATGACAG TGATAGCTGG TGCCCGCCAT TACCGGTACA AACATACTTA
    CACCAGGGTA TGGAAGATGA ACTGGAAGAA GATGATGATC GTGTCCCAAC ACCTCCTGTC
    CGAGGCGTGG CTTCTTCTCC TGCTATTTCC TTCGGACAGC AGTCCACTGC AACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACCTGGATGA GTTGACAAGA
    GCCTATCAGT TTGATATAGC GAAGCAAACA TGGCACATTC AAAGCAATAA TCAACCTCCA
    CAGCCCCCGG TTCCACCATT AGGTTACATG TCCGGAGCCT TGATTTCCGA TTTGGAAACA
    GATGTTCCAG ATGATGACGC TGATGATGAG GAGGAAGCTT TAGAAATCCC CAGACCCCTG
    AGAGCACTAG AGCAGACACC CGGATCCAGT ATGGACAATC TAGACAGCTC TGTGACAGGT
    TCAATGGTAA ATGGCTGGGG CTCTGCATCT GATGAGGATC GTAACTTTTC TAGTCATAGA
    TCTAGTGTAG GTAGCTCCTC AGATGGCTCC ATCTTTGCCA GCGGCAGCTT TGCACAAGCA
    GTGGTGGCAG CAGCAGATAA AGCTGGTTTT AGGCTGGATG GAACCAGCCT TACAAGAACA
    GGCAAAGCTT TTACCTCCTC TCAAAGACCT CGACCAACCA GCCCATTTTC TACTGACAGT
    AATACCAGTG CAGCCCTGAG TCAAAATCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA
    GGGCGAATGG ATCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGATCTT
    CCACCACCAC CAGATCCCCC TCCAGGTCAG GGTTTAAGGC AGCAAATAGG CCCGAGCCAG
    CATGCTGGTA ACATGGAAAA CTCAACAGAG AGAAAAGGAA GCTCTCTGGA GACACCACAA
    GCATCCAACT TAGAAGACAC AAAGAGCTCA TTGGATTGTC CAGCTAGAAC GTCCCTAGAG
    TGGCAGCGAC AAACCCAAGA ATGGATAAAC TCCACAGAAC GCCAAGAAGA TATACGGAAA
    GCCCTACACA AACAAAGTGT TGGACCAGAG GAAACATTGG TGCCATACAG CAAGCCCAGT
    TTCCCATCTC CGGGAGGTCA CAGCTCATCA GGAACAGCAT CTTCTAAAGG ATCCACTGGA
    CCTAGGAAAG CCGAGGCGTT GAGGGGAAGT CACCAACGCA ATGCCAGTGA CCTTCTTGAC
    ATAGGATATA TGGGCTCAAA TAGTCAAGGA CAGTTTACAG GTGAATTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe283877
    Clone ID Related Accession (Same CDS sequence) XM_022413101.1
    Accession Version XM_022413101.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4674bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product roundabout homolog 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTTACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAAAAAGA AGGCAGCCAG
    AACTTGCTTT TTCCAAATCA ACCCCAGCAA CCCAACAGTA GATGCTCTGT TTCGCCAACT
    GGAGACCTCA CAATTACCAA CATTCAGCGC TCCGATGCGG GTTACTATAT CTGCCAGGCA
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AAGTTACTGA TGTTTTAACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCTGCAAACC AAACTCTAGC AGTTGATGGT
    ACAGCATTAC TGAAATGTAA AGCTACTGGG GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCTGGG TAGAGATTCA AGAGCAACTA TACAAGAACA AGGAACATTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGTACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGGGAGA CTTCGTGGAG TGCTGTGCTG GATGTGACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ACTTAAGTGA CCTACCAGGG CCACCATCCA AACCACAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAA CCAGGTACCC CTGGAGCCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC GGTGGCAAAC
    CATGTAAAGA CCACGCTCTA TACAGTGAGA GGATTGCGGC CCAATACGAT CTATTTATTC
    ATGGTCAGAG CTATCAACCC CCAAGGTCTA AGCGACCCAA GCCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ATAGACAAGT ACAGAAAGAA
    CTAGGAGATG TCATTGTCCG TCTTCATACT CCAGTTGTGT TGACTCCCAC CACTGTTCAG
    GTCACATGGA CGGTTGATCG CCAGCCCCAG TTTATTCAAG GCTACCGAGT GATGTATCGT
    CAGACCACAG GCCTGCAGGC TACGACAACA TGGCAGAATT TAGATGCCAA AGTCCCAACC
    GAGCGAAGTG CTGTCTTGGT CAACCTGAAA AAGGGAGTGA CTTATGAAAT TAAAGTTCGA
    CCATATTTTA ATGAGTTCCA AGGAATGGAC AGTGAATCTA AAATAGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCTCC ACAATCTGTC ACTGTGCTGA CAGTTGGAAG CCACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ATCAGAATGG AATCATCCAA
    GAATACAAGA TCTGGTGTCT GGGGAATGAA ACACGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GATCTGTGAT AATTGGTGGA TTGTTCCCAG GTATTCAGTA CCGGGTAGAG
    GTTGCAGCTA GTACAAGTGC AGGGGTTGGA GTAAAAAGTG AGCCGCAGCC GATAATAATT
    GGGGGACGTA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTTGTGA AGCAACCAGC CTTTATAGCT GGAATTGGTG GTGCCTGCTG GGTAATTTTG
    ATGGGTTTTA GCATCTGGTT GTATTGGCGA AGAAAGAAGA GAAAGGGACT CAGTAACTAT
    GCTGTTACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCTGGTCTT
    CTAAATGCTG GTGATCCCAG TTACCCATGG CTTGCTGATT CATGGCCAGC CACGAGCTTA
    CCAGTAAACA ATAGCAATAG TGGCCCAAAT GAGATTGGGA ATTTTGGACG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC GACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACAAC ACAGATCTTG CATTCCAATA GCATACATGA ATTGGCTGTC
    GATCTGCCGG ACCCACAGTG GAAGAGCTCA ATTCAGCAAA AAACGGACCT GATGGGCTTT
    GGATATTCTC TCCCTGATCA GAACAAGGGT AACAACGGTG GGAAAGGTGG AAAAAAGAAG
    AAAACTAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CGACTTGGGC CAATGTACCC
    CTACCTCCTC CCCCAGTCCA GCCCCTTCCT GGTACAGAGC TGGAACACTA TGCAGTGGAA
    CCGCAAGAAA ATGGGTATGA CAGTGATAGC TGGTGCCCGC CATTACCGGT ACAAACATAC
    TTACACCAGG GTATGGAAGA TGAACTGGAA GAAGATGATG ATCGTGTCCC AACACCTCCT
    GTCCGAGGCG TGGCTTCTTC TCCTGCTATT TCCTTCGGAC AGCAGTCCAC TGCAACTCTT
    ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAAG CTCACCTGGA TGAGTTGACA
    AGAGCCTATC AGTTTGATAT AGCGAAGCAA ACATGGCACA TTCAAAGCAA TAATCAACCT
    CCACAGCCCC CGGTTCCACC ATTAGGTTAC ATGTCCGGAG CCTTGATTTC CGATTTGGAA
    ACAGATGTTC CAGATGATGA CGCTGATGAT GAGGAGGAAG CTTTAGAAAT CCCCAGACCC
    CTGAGAGCAC TAGAGCAGAC ACCCGGATCC AGTATGGACA ATCTAGACAG CTCTGTGACA
    GGTTCAATGG TAAATGGCTG GGGCTCTGCA TCTGATGAGG ATCGTAACTT TTCTAGTCAT
    AGATCTAGTG TAGGTAGCTC CTCAGATGGC TCCATCTTTG CCAGCGGCAG CTTTGCACAA
    GCAGTGGTGG CAGCAGCAGA TAAAGCTGGT TTTAGGCTGG ATGGAACCAG CCTTACAAGA
    ACAGGCAAAG CTTTTACCTC CTCTCAAAGA CCTCGACCAA CCAGCCCATT TTCTACTGAC
    AGTAATACCA GTGCAGCCCT GAGTCAAAAT CAGAGGCCTC GGCCCACTAA AAAACACAAG
    GGAGGGCGAA TGGATCAACA ACCAGCATTG CCTCATCGAA GGGAAGGAAT GACAGATGAT
    CTTCCACCAC CACCAGATCC CCCTCCAGGT CAGGGTTTAA GGCAGCAAAT AGGCCCGAGC
    CAGCATGCTG GTAACATGGA AAACTCAACA GAGAGAAAAG GAAGCTCTCT GGAGACACCA
    CAAGCATCCA ACTTAGAAGA CACAAAGAGC TCATTGGATT GTCCAGCTAG AACGTCCCTA
    GAGTGGCAGC GACAAACCCA AGAATGGATA AACTCCACAG AACGCCAAGA AGATATACGG
    AAAGCCCTAC ACAAACAAAG TGTTGGACCA GAGGAAACAT TGGTGCCATA CAGCAAGCCC
    AGTTTCCCAT CTCCGGGAGG TCACAGCTCA TCAGGAACAG CATCTTCTAA AGGATCCACT
    GGACCTAGGA AAGCCGAGGC GTTGAGGGGA AGTCACCAAC GCAATGCCAG TGACCTTCTT
    GACATAGGAT ATATGGGCTC AAATAGTCAA GGACAGTTTA CAGGTGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268809.1
    CDS386..5059
    Translation

    Target ORF information:

    RefSeq Version XM_022413101.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413101.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTTACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAAAAAGA AGGCAGCCAG
    AACTTGCTTT TTCCAAATCA ACCCCAGCAA CCCAACAGTA GATGCTCTGT TTCGCCAACT
    GGAGACCTCA CAATTACCAA CATTCAGCGC TCCGATGCGG GTTACTATAT CTGCCAGGCA
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AAGTTACTGA TGTTTTAACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCTGCAAACC AAACTCTAGC AGTTGATGGT
    ACAGCATTAC TGAAATGTAA AGCTACTGGG GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCTGGG TAGAGATTCA AGAGCAACTA TACAAGAACA AGGAACATTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGTACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGGGAGA CTTCGTGGAG TGCTGTGCTG GATGTGACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ACTTAAGTGA CCTACCAGGG CCACCATCCA AACCACAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAA CCAGGTACCC CTGGAGCCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC GGTGGCAAAC
    CATGTAAAGA CCACGCTCTA TACAGTGAGA GGATTGCGGC CCAATACGAT CTATTTATTC
    ATGGTCAGAG CTATCAACCC CCAAGGTCTA AGCGACCCAA GCCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ATAGACAAGT ACAGAAAGAA
    CTAGGAGATG TCATTGTCCG TCTTCATACT CCAGTTGTGT TGACTCCCAC CACTGTTCAG
    GTCACATGGA CGGTTGATCG CCAGCCCCAG TTTATTCAAG GCTACCGAGT GATGTATCGT
    CAGACCACAG GCCTGCAGGC TACGACAACA TGGCAGAATT TAGATGCCAA AGTCCCAACC
    GAGCGAAGTG CTGTCTTGGT CAACCTGAAA AAGGGAGTGA CTTATGAAAT TAAAGTTCGA
    CCATATTTTA ATGAGTTCCA AGGAATGGAC AGTGAATCTA AAATAGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCTCC ACAATCTGTC ACTGTGCTGA CAGTTGGAAG CCACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ATCAGAATGG AATCATCCAA
    GAATACAAGA TCTGGTGTCT GGGGAATGAA ACACGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GATCTGTGAT AATTGGTGGA TTGTTCCCAG GTATTCAGTA CCGGGTAGAG
    GTTGCAGCTA GTACAAGTGC AGGGGTTGGA GTAAAAAGTG AGCCGCAGCC GATAATAATT
    GGGGGACGTA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTTGTGA AGCAACCAGC CTTTATAGCT GGAATTGGTG GTGCCTGCTG GGTAATTTTG
    ATGGGTTTTA GCATCTGGTT GTATTGGCGA AGAAAGAAGA GAAAGGGACT CAGTAACTAT
    GCTGTTACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCTGGTCTT
    CTAAATGCTG GTGATCCCAG TTACCCATGG CTTGCTGATT CATGGCCAGC CACGAGCTTA
    CCAGTAAACA ATAGCAATAG TGGCCCAAAT GAGATTGGGA ATTTTGGACG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC GACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACAAC ACAGATCTTG CATTCCAATA GCATACATGA ATTGGCTGTC
    GATCTGCCGG ACCCACAGTG GAAGAGCTCA ATTCAGCAAA AAACGGACCT GATGGGCTTT
    GGATATTCTC TCCCTGATCA GAACAAGGGT AACAACGGTG GGAAAGGTGG AAAAAAGAAG
    AAAACTAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CGACTTGGGC CAATGTACCC
    CTACCTCCTC CCCCAGTCCA GCCCCTTCCT GGTACAGAGC TGGAACACTA TGCAGTGGAA
    CCGCAAGAAA ATGGGTATGA CAGTGATAGC TGGTGCCCGC CATTACCGGT ACAAACATAC
    TTACACCAGG GTATGGAAGA TGAACTGGAA GAAGATGATG ATCGTGTCCC AACACCTCCT
    GTCCGAGGCG TGGCTTCTTC TCCTGCTATT TCCTTCGGAC AGCAGTCCAC TGCAACTCTT
    ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAAG CTCACCTGGA TGAGTTGACA
    AGAGCCTATC AGTTTGATAT AGCGAAGCAA ACATGGCACA TTCAAAGCAA TAATCAACCT
    CCACAGCCCC CGGTTCCACC ATTAGGTTAC ATGTCCGGAG CCTTGATTTC CGATTTGGAA
    ACAGATGTTC CAGATGATGA CGCTGATGAT GAGGAGGAAG CTTTAGAAAT CCCCAGACCC
    CTGAGAGCAC TAGAGCAGAC ACCCGGATCC AGTATGGACA ATCTAGACAG CTCTGTGACA
    GGTTCAATGG TAAATGGCTG GGGCTCTGCA TCTGATGAGG ATCGTAACTT TTCTAGTCAT
    AGATCTAGTG TAGGTAGCTC CTCAGATGGC TCCATCTTTG CCAGCGGCAG CTTTGCACAA
    GCAGTGGTGG CAGCAGCAGA TAAAGCTGGT TTTAGGCTGG ATGGAACCAG CCTTACAAGA
    ACAGGCAAAG CTTTTACCTC CTCTCAAAGA CCTCGACCAA CCAGCCCATT TTCTACTGAC
    AGTAATACCA GTGCAGCCCT GAGTCAAAAT CAGAGGCCTC GGCCCACTAA AAAACACAAG
    GGAGGGCGAA TGGATCAACA ACCAGCATTG CCTCATCGAA GGGAAGGAAT GACAGATGAT
    CTTCCACCAC CACCAGATCC CCCTCCAGGT CAGGGTTTAA GGCAGCAAAT AGGCCCGAGC
    CAGCATGCTG GTAACATGGA AAACTCAACA GAGAGAAAAG GAAGCTCTCT GGAGACACCA
    CAAGCATCCA ACTTAGAAGA CACAAAGAGC TCATTGGATT GTCCAGCTAG AACGTCCCTA
    GAGTGGCAGC GACAAACCCA AGAATGGATA AACTCCACAG AACGCCAAGA AGATATACGG
    AAAGCCCTAC ACAAACAAAG TGTTGGACCA GAGGAAACAT TGGTGCCATA CAGCAAGCCC
    AGTTTCCCAT CTCCGGGAGG TCACAGCTCA TCAGGAACAG CATCTTCTAA AGGATCCACT
    GGACCTAGGA AAGCCGAGGC GTTGAGGGGA AGTCACCAAC GCAATGCCAG TGACCTTCTT
    GACATAGGAT ATATGGGCTC AAATAGTCAA GGACAGTTTA CAGGTGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe283878
    Clone ID Related Accession (Same CDS sequence) XM_022413102.1
    Accession Version XM_022413102.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4527bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product roundabout homolog 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTTACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAAAAAGA AGGCAGCCAG
    AACTTGCTTT TTCCAAATCA ACCCCAGCAA CCCAACAGTA GATGCTCTGT TTCGCCAACT
    GGAGACCTCA CAATTACCAA CATTCAGCGC TCCGATGCGG GTTACTATAT CTGCCAGGCA
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AAGTTACTGA TGTTTTAACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCTGCAAACC AAACTCTAGC AGTTGATGGT
    ACAGCATTAC TGAAATGTAA AGCTACTGGG GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCTGGG TAGAGATTCA AGAGCAACTA TACAAGAACA AGGAACATTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGTACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGGGAGA CTTCGTGGAG TGCTGTGCTG GATGTGACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ACTTAAGTGA CCTACCAGGG CCACCATCCA AACCACAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAA CCAGGTACCC CTGGAGCCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC GGTGGCAAAC
    CATGTAAAGA CCACGCTCTA TACAGTGAGA GGATTGCGGC CCAATACGAT CTATTTATTC
    ATGGTCAGAG CTATCAACCC CCAAGGTCTA AGCGACCCAA GCCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ATAGACAAGT ACAGAAAGAA
    CTAGGAGATG TCATTGTCCG TCTTCATACT CCAGTTGTGT TGACTCCCAC CACTGTTCAG
    GTCACATGGA CGGTTGATCG CCAGCCCCAG TTTATTCAAG GCTACCGAGT GATGTATCGT
    CAGACCACAG GCCTGCAGGC TACGACAACA TGGCAGAATT TAGATGCCAA AGTCCCAACC
    GAGCGAAGTG CTGTCTTGGT CAACCTGAAA AAGGGAGTGA CTTATGAAAT TAAAGTTCGA
    CCATATTTTA ATGAGTTCCA AGGAATGGAC AGTGAATCTA AAATAGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCTCC ACAATCTGTC ACTGTGCTGA CAGTTGGAAG CCACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ATCAGAATGG AATCATCCAA
    GAATACAAGA TCTGGTGTCT GGGGAATGAA ACACGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GATCTGTGAT AATTGGTGGA TTGTTCCCAG GTATTCAGTA CCGGGTAGAG
    GTTGCAGCTA GTACAAGTGC AGGGGTTGGA GTAAAAAGTG AGCCGCAGCC GATAATAATT
    GGGGGACGTA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTTGTGA AGCAACCAGC CTTTATAGCT GGAATTGGTG GTGCCTGCTG GGTAATTTTG
    ATGGGTTTTA GCATCTGGTT GTATTGGCGA AGAAAGAAGA GAAAGGGACT CAGTAACTAT
    GCTGTTACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCTGGTCTT
    CTAAATGCTG GTGATCCCAG TTACCCATGG CTTGCTGATT CATGGCCAGC CACGAGCTTA
    CCAGTAAACA ATAGCAATAG TGGCCCAAAT GAGATTGGGA ATTTTGGACG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC GACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACAAC ACAGATCTTG CATTCCAATA GCATACATGA ATTGGCTGTC
    GATCTGCCGG ACCCACAGTG GAAGAGCTCA ATTCAGCAAA AAACGGACCT GATGGGCTTT
    GGATATTCTC TCCCTGATCA GAACAAGGGT AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAGAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCGAC TTGGGCCAAT
    GTACCCCTAC CTCCTCCCCC AGTCCAGCCC CTTCCTGGTA CAGAGCTGGA ACACTATGCA
    GTGGAACCGC AAGAAAATGG GTATGACAGT GATAGCTGGT GCCCGCCATT ACCGGTACAA
    ACATACTTAC ACCAGGGTAT GGAAGATGAA CTGGAAGAAG ATGATGATCG TGTCCCAACA
    CCTCCTGTCC GAGGCGTGGC TTCTTCTCCT GCTATTTCCT TCGGACAGCA GTCCACTGCA
    ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAAGCTCA CCTGGATGAG
    TTGACAAGAG CCTATCAGTT TGATATAGCG AAGCAAACAT GGCACATTCA AAGCAATAAT
    CAACCTCCAC AGCCCCCGGT TCCACCATTA GGTTACATGT CCGGAGCCTT GATTTCCGAT
    TTGGAAACAG ATGTTCCAGA TGATGACGCT GATGATGAGG AGGAAGCTTT AGAAATCCCC
    AGACCCCTGA GAGCACTAGA GCAGACACCC GGATCCAGTA TGGACAATCT AGACAGCTCT
    GTGACAGGTT CAATGGTAAA TGGCTGGGGC TCTGCATCTG ATGAGGATCG TAACTTTTCT
    AGTCATAGAT CTAGTGTAGG TAGCTCCTCA GATGGCTCCA TCTTTGCCAG CGGCAGCTTT
    GCACAAGCAG TGGTGGCAGC AGCAGATAAA GCTGGTTTTA GGCTGGATGG AACCAGCCTT
    ACAAGAACAG GCAAAGCTTT TACCTCCTCT CAAAGACCTC GACCAACCAG CCCATTTTCT
    ACTGACAGTA ATACCAGTGC AGCCCTGAGT CAAAATCAGA GGCCTCGGCC CACTAAAAAA
    CACAAGGGAG GGCGAATGGA TCAACAACCA GCATTGCCTC ATCGAAGGGA AGGAATGACA
    GATGAGGAAA CATTGGTGCC ATACAGCAAG CCCAGTTTCC CATCTCCGGG AGGTCACAGC
    TCATCAGGAA CAGCATCTTC TAAAGGATCC ACTGGACCTA GGAAAGCCGA GGCGTTGAGG
    GGAAGTCACC AACGCAATGC CAGTGACCTT CTTGACATAG GATATATGGG CTCAAATAGT
    CAAGGACAGT TTACAGGTGA ATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268810.1
    CDS388..4914
    Translation

    Target ORF information:

    RefSeq Version XM_022413102.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413102.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTTACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAAAAAGA AGGCAGCCAG
    AACTTGCTTT TTCCAAATCA ACCCCAGCAA CCCAACAGTA GATGCTCTGT TTCGCCAACT
    GGAGACCTCA CAATTACCAA CATTCAGCGC TCCGATGCGG GTTACTATAT CTGCCAGGCA
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AAGTTACTGA TGTTTTAACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCTGCAAACC AAACTCTAGC AGTTGATGGT
    ACAGCATTAC TGAAATGTAA AGCTACTGGG GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCTGGG TAGAGATTCA AGAGCAACTA TACAAGAACA AGGAACATTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGTACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGGGAGA CTTCGTGGAG TGCTGTGCTG GATGTGACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ACTTAAGTGA CCTACCAGGG CCACCATCCA AACCACAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAA CCAGGTACCC CTGGAGCCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC GGTGGCAAAC
    CATGTAAAGA CCACGCTCTA TACAGTGAGA GGATTGCGGC CCAATACGAT CTATTTATTC
    ATGGTCAGAG CTATCAACCC CCAAGGTCTA AGCGACCCAA GCCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ATAGACAAGT ACAGAAAGAA
    CTAGGAGATG TCATTGTCCG TCTTCATACT CCAGTTGTGT TGACTCCCAC CACTGTTCAG
    GTCACATGGA CGGTTGATCG CCAGCCCCAG TTTATTCAAG GCTACCGAGT GATGTATCGT
    CAGACCACAG GCCTGCAGGC TACGACAACA TGGCAGAATT TAGATGCCAA AGTCCCAACC
    GAGCGAAGTG CTGTCTTGGT CAACCTGAAA AAGGGAGTGA CTTATGAAAT TAAAGTTCGA
    CCATATTTTA ATGAGTTCCA AGGAATGGAC AGTGAATCTA AAATAGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCTCC ACAATCTGTC ACTGTGCTGA CAGTTGGAAG CCACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ATCAGAATGG AATCATCCAA
    GAATACAAGA TCTGGTGTCT GGGGAATGAA ACACGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GATCTGTGAT AATTGGTGGA TTGTTCCCAG GTATTCAGTA CCGGGTAGAG
    GTTGCAGCTA GTACAAGTGC AGGGGTTGGA GTAAAAAGTG AGCCGCAGCC GATAATAATT
    GGGGGACGTA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTTGTGA AGCAACCAGC CTTTATAGCT GGAATTGGTG GTGCCTGCTG GGTAATTTTG
    ATGGGTTTTA GCATCTGGTT GTATTGGCGA AGAAAGAAGA GAAAGGGACT CAGTAACTAT
    GCTGTTACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCTGGTCTT
    CTAAATGCTG GTGATCCCAG TTACCCATGG CTTGCTGATT CATGGCCAGC CACGAGCTTA
    CCAGTAAACA ATAGCAATAG TGGCCCAAAT GAGATTGGGA ATTTTGGACG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC GACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACAAC ACAGATCTTG CATTCCAATA GCATACATGA ATTGGCTGTC
    GATCTGCCGG ACCCACAGTG GAAGAGCTCA ATTCAGCAAA AAACGGACCT GATGGGCTTT
    GGATATTCTC TCCCTGATCA GAACAAGGGT AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAGAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCGAC TTGGGCCAAT
    GTACCCCTAC CTCCTCCCCC AGTCCAGCCC CTTCCTGGTA CAGAGCTGGA ACACTATGCA
    GTGGAACCGC AAGAAAATGG GTATGACAGT GATAGCTGGT GCCCGCCATT ACCGGTACAA
    ACATACTTAC ACCAGGGTAT GGAAGATGAA CTGGAAGAAG ATGATGATCG TGTCCCAACA
    CCTCCTGTCC GAGGCGTGGC TTCTTCTCCT GCTATTTCCT TCGGACAGCA GTCCACTGCA
    ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAAGCTCA CCTGGATGAG
    TTGACAAGAG CCTATCAGTT TGATATAGCG AAGCAAACAT GGCACATTCA AAGCAATAAT
    CAACCTCCAC AGCCCCCGGT TCCACCATTA GGTTACATGT CCGGAGCCTT GATTTCCGAT
    TTGGAAACAG ATGTTCCAGA TGATGACGCT GATGATGAGG AGGAAGCTTT AGAAATCCCC
    AGACCCCTGA GAGCACTAGA GCAGACACCC GGATCCAGTA TGGACAATCT AGACAGCTCT
    GTGACAGGTT CAATGGTAAA TGGCTGGGGC TCTGCATCTG ATGAGGATCG TAACTTTTCT
    AGTCATAGAT CTAGTGTAGG TAGCTCCTCA GATGGCTCCA TCTTTGCCAG CGGCAGCTTT
    GCACAAGCAG TGGTGGCAGC AGCAGATAAA GCTGGTTTTA GGCTGGATGG AACCAGCCTT
    ACAAGAACAG GCAAAGCTTT TACCTCCTCT CAAAGACCTC GACCAACCAG CCCATTTTCT
    ACTGACAGTA ATACCAGTGC AGCCCTGAGT CAAAATCAGA GGCCTCGGCC CACTAAAAAA
    CACAAGGGAG GGCGAATGGA TCAACAACCA GCATTGCCTC ATCGAAGGGA AGGAATGACA
    GATGAGGAAA CATTGGTGCC ATACAGCAAG CCCAGTTTCC CATCTCCGGG AGGTCACAGC
    TCATCAGGAA CAGCATCTTC TAAAGGATCC ACTGGACCTA GGAAAGCCGA GGCGTTGAGG
    GGAAGTCACC AACGCAATGC CAGTGACCTT CTTGACATAG GATATATGGG CTCAAATAGT
    CAAGGACAGT TTACAGGTGA ATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29283
    Clone ID Related Accession (Same CDS sequence) XM_022413097.1
    Accession Version XM_022413097.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4794bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product roundabout homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTTACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAAAAAGA AGGCAGCCAG
    AACTTGCTTT TTCCAAATCA ACCCCAGCAA CCCAACAGTA GATGCTCTGT TTCGCCAACT
    GGAGACCTCA CAATTACCAA CATTCAGCGC TCCGATGCGG GTTACTATAT CTGCCAGGCA
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AAGTTACTGA TGTTTTAACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCTGCAAACC AAACTCTAGC AGTTGATGGT
    ACAGCATTAC TGAAATGTAA AGCTACTGGG GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCTGGG TAGAGATTCA AGAGCAACTA TACAAGAACA AGGAACATTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGTACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGGGAGA CTTCGTGGAG TGCTGTGCTG GATGTGACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ACTTAAGTGA CCTACCAGGG CCACCATCCA AACCACAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAA CCAGGTACCC CTGGAGCCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC GGTGGCAAAC
    CATGTAAAGA CCACGCTCTA TACAGTGAGA GGATTGCGGC CCAATACGAT CTATTTATTC
    ATGGTCAGAG CTATCAACCC CCAAGGTCTA AGCGACCCAA GCCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ATAGACAAGT ACAGAAAGAA
    CTAGGAGATG TCATTGTCCG TCTTCATACT CCAGTTGTGT TGACTCCCAC CACTGTTCAG
    GTCACATGGA CGGTTGATCG CCAGCCCCAG TTTATTCAAG GCTACCGAGT GATGTATCGT
    CAGACCACAG GCCTGCAGGC TACGACAACA TGGCAGAATT TAGATGCCAA AGTCCCAACC
    GAGCGAAGTG CTGTCTTGGT CAACCTGAAA AAGGGAGTGA CTTATGAAAT TAAAGTTCGA
    CCATATTTTA ATGAGTTCCA AGGAATGGAC AGTGAATCTA AAATAGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCTCC ACAATCTGTC ACTGTGCTGA CAGTTGGAAG CCACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ATCAGAATGG AATCATCCAA
    GAATACAAGA TCTGGTGTCT GGGGAATGAA ACACGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GATCTGTGAT AATTGGTGGA TTGTTCCCAG GTATTCAGTA CCGGGTAGAG
    GTTGCAGCTA GTACAAGTGC AGGGGTTGGA GTAAAAAGTG AGCCGCAGCC GATAATAATT
    GGGGGACGTA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTTGTGA AGCAACCAGC CTTTATAGCT GGAATTGGTG GTGCCTGCTG GGTAATTTTG
    ATGGGTTTTA GCATCTGGTT GTATTGGCGA AGAAAGAAGA GAAAGGGACT CAGTAACTAT
    GCTGTTACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCTGGTCTT
    CTAAATGCTG GTGATCCCAG TTACCCATGG CTTGCTGATT CATGGCCAGC CACGAGCTTA
    CCAGTAAACA ATAGCAATAG TGGCCCAAAT GAGATTGGGA ATTTTGGACG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC GACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACAAC ACAGATCTTG CATTCCAATA GCATACATGA ATTGGCTGTC
    GATCTGCCGG ACCCACAGTG GAAGAGCTCA ATTCAGCAAA AAACGGACCT GATGGGCTTT
    GGATATTCTC TCCCTGATCA GAACAAGGGT AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAGAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCGAC TTGGGCCAAT
    GTACCCCTAC CTCCTCCCCC AGTCCAGCCC CTTCCTGGTA CAGAGCTGGA ACACTATGCA
    GTGGAACCGC AAGAAAATGG GTATGACAGT GATAGCTGGT GCCCGCCATT ACCGGTACAA
    ACATACTTAC ACCAGGGTAT GGAAGATGAA CTGGAAGAAG ATGATGATCG TGTCCCAACA
    CCTCCTGTCC GAGGCGTGGC TTCTTCTCCT GCTATTTCCT TCGGACAGCA GTCCACTGCA
    ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAAGCTCA CCTGGATGAG
    TTGACAAGAG CCTATCAGTT TGATATAGCG AAGCAAACAT GGCACATTCA AAGCAATAAT
    CAACCTCCAC AGCCCCCGGT TCCACCATTA GGTTACATGT CCGGAGCCTT GATTTCCGAT
    TTGGAAACAG ATGTTCCAGA TGATGACGCT GATGATGAGG AGGAAGCTTT AGAAATCCCC
    AGACCCCTGA GAGCACTAGA GCAGACACCC GGATCCAGTA TGGACAATCT AGACAGCTCT
    GTGACAGGTT CAATGGTAAA TGGCTGGGGC TCTGCATCTG ATGAGGATCG TAACTTTTCT
    AGTCATAGAT CTAGTGTAGG TAGCTCCTCA GATGGCTCCA TCTTTGCCAG CGGCAGCTTT
    GCACAAGCAG TGGTGGCAGC AGCAGATAAA GCTGGTTTTA GGCTGGATGG AACCAGCCTT
    ACAAGAACAG GCAAAGCTTT TACCTCCTCT CAAAGACCTC GACCAACCAG CCCATTTTCT
    ACTGACAGTA ATACCAGTGC AGCCCTGAGT CAAAATCAGA GGCCTCGGCC CACTAAAAAA
    CACAAGGGAG GGCGAATGGA TCAACAACCA GCATTGCCTC ATCGAAGGGA AGGAATGACA
    GATGATCTTC CACCACCACC AGATCCCCCT CCAGGTCAGG GTTTAAGGCA GCAAATAGGC
    CCGAGCCAGC ATGCTGGTAA CATGGAAAAC TCAACAGAGA GAAAAGGAAG CTCTCTGGAG
    ACACCACAAG CATCCAACTT AGAAGACACA AAGAGCTCAT TGGATTGTCC AGCTAGAACG
    TCCCTAGAGT GGCAGCGACA AACCCAAGAA TGGATAAACT CCACAGAACG CCAAGAAGAT
    ATACGGAAAG CCCTACACAA ACAAAGTGTT GGACCAGAGG AAACATTGGT GCCATACAGC
    AAGCCCAGTT TCCCATCTCC GGGAGGTCAC AGCTCATCAG GAACAGCATC TTCTAAAGGA
    TCCACTGGAC CTAGGAAAGC CGAGGCGTTG AGGGGAAGTC ACCAACGCAA TGCCAGTGAC
    CTTCTTGACA TAGGATATAT GGGCTCAAAT AGTCAAGGAC AGTTTACAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268805.1
    CDS388..5181
    Translation

    Target ORF information:

    RefSeq Version XM_022413097.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413097.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTTACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAAAAAGA AGGCAGCCAG
    AACTTGCTTT TTCCAAATCA ACCCCAGCAA CCCAACAGTA GATGCTCTGT TTCGCCAACT
    GGAGACCTCA CAATTACCAA CATTCAGCGC TCCGATGCGG GTTACTATAT CTGCCAGGCA
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AAGTTACTGA TGTTTTAACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCTGCAAACC AAACTCTAGC AGTTGATGGT
    ACAGCATTAC TGAAATGTAA AGCTACTGGG GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCTGGG TAGAGATTCA AGAGCAACTA TACAAGAACA AGGAACATTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGTACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGGGAGA CTTCGTGGAG TGCTGTGCTG GATGTGACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ACTTAAGTGA CCTACCAGGG CCACCATCCA AACCACAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAA CCAGGTACCC CTGGAGCCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC GGTGGCAAAC
    CATGTAAAGA CCACGCTCTA TACAGTGAGA GGATTGCGGC CCAATACGAT CTATTTATTC
    ATGGTCAGAG CTATCAACCC CCAAGGTCTA AGCGACCCAA GCCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ATAGACAAGT ACAGAAAGAA
    CTAGGAGATG TCATTGTCCG TCTTCATACT CCAGTTGTGT TGACTCCCAC CACTGTTCAG
    GTCACATGGA CGGTTGATCG CCAGCCCCAG TTTATTCAAG GCTACCGAGT GATGTATCGT
    CAGACCACAG GCCTGCAGGC TACGACAACA TGGCAGAATT TAGATGCCAA AGTCCCAACC
    GAGCGAAGTG CTGTCTTGGT CAACCTGAAA AAGGGAGTGA CTTATGAAAT TAAAGTTCGA
    CCATATTTTA ATGAGTTCCA AGGAATGGAC AGTGAATCTA AAATAGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCTCC ACAATCTGTC ACTGTGCTGA CAGTTGGAAG CCACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ATCAGAATGG AATCATCCAA
    GAATACAAGA TCTGGTGTCT GGGGAATGAA ACACGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GATCTGTGAT AATTGGTGGA TTGTTCCCAG GTATTCAGTA CCGGGTAGAG
    GTTGCAGCTA GTACAAGTGC AGGGGTTGGA GTAAAAAGTG AGCCGCAGCC GATAATAATT
    GGGGGACGTA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTTGTGA AGCAACCAGC CTTTATAGCT GGAATTGGTG GTGCCTGCTG GGTAATTTTG
    ATGGGTTTTA GCATCTGGTT GTATTGGCGA AGAAAGAAGA GAAAGGGACT CAGTAACTAT
    GCTGTTACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCTGGTCTT
    CTAAATGCTG GTGATCCCAG TTACCCATGG CTTGCTGATT CATGGCCAGC CACGAGCTTA
    CCAGTAAACA ATAGCAATAG TGGCCCAAAT GAGATTGGGA ATTTTGGACG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC GACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACAAC ACAGATCTTG CATTCCAATA GCATACATGA ATTGGCTGTC
    GATCTGCCGG ACCCACAGTG GAAGAGCTCA ATTCAGCAAA AAACGGACCT GATGGGCTTT
    GGATATTCTC TCCCTGATCA GAACAAGGGT AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAGAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCGAC TTGGGCCAAT
    GTACCCCTAC CTCCTCCCCC AGTCCAGCCC CTTCCTGGTA CAGAGCTGGA ACACTATGCA
    GTGGAACCGC AAGAAAATGG GTATGACAGT GATAGCTGGT GCCCGCCATT ACCGGTACAA
    ACATACTTAC ACCAGGGTAT GGAAGATGAA CTGGAAGAAG ATGATGATCG TGTCCCAACA
    CCTCCTGTCC GAGGCGTGGC TTCTTCTCCT GCTATTTCCT TCGGACAGCA GTCCACTGCA
    ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAAGCTCA CCTGGATGAG
    TTGACAAGAG CCTATCAGTT TGATATAGCG AAGCAAACAT GGCACATTCA AAGCAATAAT
    CAACCTCCAC AGCCCCCGGT TCCACCATTA GGTTACATGT CCGGAGCCTT GATTTCCGAT
    TTGGAAACAG ATGTTCCAGA TGATGACGCT GATGATGAGG AGGAAGCTTT AGAAATCCCC
    AGACCCCTGA GAGCACTAGA GCAGACACCC GGATCCAGTA TGGACAATCT AGACAGCTCT
    GTGACAGGTT CAATGGTAAA TGGCTGGGGC TCTGCATCTG ATGAGGATCG TAACTTTTCT
    AGTCATAGAT CTAGTGTAGG TAGCTCCTCA GATGGCTCCA TCTTTGCCAG CGGCAGCTTT
    GCACAAGCAG TGGTGGCAGC AGCAGATAAA GCTGGTTTTA GGCTGGATGG AACCAGCCTT
    ACAAGAACAG GCAAAGCTTT TACCTCCTCT CAAAGACCTC GACCAACCAG CCCATTTTCT
    ACTGACAGTA ATACCAGTGC AGCCCTGAGT CAAAATCAGA GGCCTCGGCC CACTAAAAAA
    CACAAGGGAG GGCGAATGGA TCAACAACCA GCATTGCCTC ATCGAAGGGA AGGAATGACA
    GATGATCTTC CACCACCACC AGATCCCCCT CCAGGTCAGG GTTTAAGGCA GCAAATAGGC
    CCGAGCCAGC ATGCTGGTAA CATGGAAAAC TCAACAGAGA GAAAAGGAAG CTCTCTGGAG
    ACACCACAAG CATCCAACTT AGAAGACACA AAGAGCTCAT TGGATTGTCC AGCTAGAACG
    TCCCTAGAGT GGCAGCGACA AACCCAAGAA TGGATAAACT CCACAGAACG CCAAGAAGAT
    ATACGGAAAG CCCTACACAA ACAAAGTGTT GGACCAGAGG AAACATTGGT GCCATACAGC
    AAGCCCAGTT TCCCATCTCC GGGAGGTCAC AGCTCATCAG GAACAGCATC TTCTAAAGGA
    TCCACTGGAC CTAGGAAAGC CGAGGCGTTG AGGGGAAGTC ACCAACGCAA TGCCAGTGAC
    CTTCTTGACA TAGGATATAT GGGCTCAAAT AGTCAAGGAC AGTTTACAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44449
    Clone ID Related Accession (Same CDS sequence) XM_014109730.1 , XM_014109730.2
    Accession Version XM_014109730.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4617bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product roundabout homolog 2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTTACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAAAAAGA AGGCAGCCAG
    AACTTGCTTT TTCCAAATCA ACCCCAGCAA CCCAACAGTA GATGCTCTGT TTCGCCAACT
    GGAGACCTCA CAATTACCAA CATTCAGCGC TCCGATGCGG GTTACTATAT CTGCCAGGCA
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AAGTTACTGA TGTTTTAACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCTGCAAACC AAACTCTAGC AGTTGATGGT
    ACAGCATTAC TGAAATGTAA AGCTACTGGG GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCTGGG TAGAGATTCA AGAGCAACTA TACAAGAACA AGGAACATTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGTACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGGGAGA CTTCGTGGAG TGCTGTGCTG GATGTGACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ACTTAAGTGA CCTACCAGGG CCACCATCCA AACCACAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAA CCAGGTACCC CTGGAGCCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC GGTGGCAAAC
    CATGTAAAGA CCACGCTCTA TACAGTGAGA GGATTGCGGC CCAATACGAT CTATTTATTC
    ATGGTCAGAG CTATCAACCC CCAAGGTCTA AGCGACCCAA GCCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ATAGACAAGT ACAGAAAGAA
    CTAGGAGATG TCATTGTCCG TCTTCATACT CCAGTTGTGT TGACTCCCAC CACTGTTCAG
    GTCACATGGA CGGTTGATCG CCAGCCCCAG TTTATTCAAG GCTACCGAGT GATGTATCGT
    CAGACCACAG GCCTGCAGGC TACGACAACA TGGCAGAATT TAGATGCCAA AGTCCCAACC
    GAGCGAAGTG CTGTCTTGGT CAACCTGAAA AAGGGAGTGA CTTATGAAAT TAAAGTTCGA
    CCATATTTTA ATGAGTTCCA AGGAATGGAC AGTGAATCTA AAATAGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCTCC ACAATCTGTC ACTGTGCTGA CAGTTGGAAG CCACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ATCAGAATGG AATCATCCAA
    GAATACAAGA TCTGGTGTCT GGGGAATGAA ACACGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GATCTGTGAT AATTGGTGGA TTGTTCCCAG GTATTCAGTA CCGGGTAGAG
    GTTGCAGCTA GTACAAGTGC AGGGGTTGGA GTAAAAAGTG AGCCGCAGCC GATAATAATT
    GGGGGACGTA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTTGTGA AGCAACCAGC CTTTATAGCT GGAATTGGTG GTGCCTGCTG GGTAATTTTG
    ATGGGTTTTA GCATCTGGTT GTATTGGCGA AGAAAGAAGA GAAAGGGACT CAGTAACTAT
    GCTGTTACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCTGGTCTT
    CTAAATGCTG GTGATCCCAG TTACCCATGG CTTGCTGATT CATGGCCAGC CACGAGCTTA
    CCAGTAAACA ATAGCAATAG TGGCCCAAAT GAGATTGGGA ATTTTGGACG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC GACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACAAC ACAGATCTTG CATTCCAATA GCATACATGA ATTGGCTGTC
    GATCTGCCGG ACCCACAGTG GAAGAGCTCA ATTCAGCAAA AAACGGACCT GATGGGCTTT
    GGATATTCTC TCCCTGATCA GAACAAGGGT AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAGAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCGAC TTGGGCCAAT
    GTACCCCTAC CTCCTCCCCC AGTCCAGCCC CTTCCTGGTA CAGAGCTGGA ACACTATGCA
    GTGGAACCGC AAGAAAATGG GTATGACAGT GATAGCTGGT GCCCGCCATT ACCGGTACAA
    ACATACTTAC ACCAGGGTAT GGAAGATGAA CTGGAAGAAG ATGATGATCG TGTCCCAACA
    CCTCCTGTCC GAGGCGTGGC TTCTTCTCCT GCTATTTCCT TCGGACAGCA GTCCACTGCA
    ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAAGCTCA CCTGGATGAG
    TTGACAAGAG CCTATCAGTT TGATATAGCG AAGCAAACAT GGCACATTCA AAGCAATAAT
    CAACCTCCAC AGCCCCCGGT TCCACCATTA GGTTACATGT CCGGAGCCTT GATTTCCGAT
    TTGGAAACAG ATGTTCCAGA TGATGACGCT GATGATGAGG AGGAAGCTTT AGAAATCCCC
    AGACCCCTGA GAGCACTAGA GCAGACACCC GGATCCAGTA TGGACAATCT AGACAGCTCT
    GTGACAGGCA AAGCTTTTAC CTCCTCTCAA AGACCTCGAC CAACCAGCCC ATTTTCTACT
    GACAGTAATA CCAGTGCAGC CCTGAGTCAA AATCAGAGGC CTCGGCCCAC TAAAAAACAC
    AAGGGAGGGC GAATGGATCA ACAACCAGCA TTGCCTCATC GAAGGGAAGG AATGACAGAT
    GATCTTCCAC CACCACCAGA TCCCCCTCCA GGTCAGGGTT TAAGGCAGCA AATAGGCCCG
    AGCCAGCATG CTGGTAACAT GGAAAACTCA ACAGAGAGAA AAGGAAGCTC TCTGGAGACA
    CCACAAGCAT CCAACTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAGAACGTCC
    CTAGAGTGGC AGCGACAAAC CCAAGAATGG ATAAACTCCA CAGAACGCCA AGAAGATATA
    CGGAAAGCCC TACACAAACA AAGTGTTGGA CCAGAGGAAA CATTGGTGCC ATACAGCAAG
    CCCAGTTTCC CATCTCCGGG AGGTCACAGC TCATCAGGAA CAGCATCTTC TAAAGGATCC
    ACTGGACCTA GGAAAGCCGA GGCGTTGAGG GGAAGTCACC AACGCAATGC CAGTGACCTT
    CTTGACATAG GATATATGGG CTCAAATAGT CAAGGACAGT TTACAGGTGA ATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965205.1
    CDS585..5201
    Translation

    Target ORF information:

    RefSeq Version XM_014109730.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109730.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTTACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAAAAAGA AGGCAGCCAG
    AACTTGCTTT TTCCAAATCA ACCCCAGCAA CCCAACAGTA GATGCTCTGT TTCGCCAACT
    GGAGACCTCA CAATTACCAA CATTCAGCGC TCCGATGCGG GTTACTATAT CTGCCAGGCA
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AAGTTACTGA TGTTTTAACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCTGCAAACC AAACTCTAGC AGTTGATGGT
    ACAGCATTAC TGAAATGTAA AGCTACTGGG GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCTGGG TAGAGATTCA AGAGCAACTA TACAAGAACA AGGAACATTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGTACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGGGAGA CTTCGTGGAG TGCTGTGCTG GATGTGACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ACTTAAGTGA CCTACCAGGG CCACCATCCA AACCACAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAA CCAGGTACCC CTGGAGCCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC GGTGGCAAAC
    CATGTAAAGA CCACGCTCTA TACAGTGAGA GGATTGCGGC CCAATACGAT CTATTTATTC
    ATGGTCAGAG CTATCAACCC CCAAGGTCTA AGCGACCCAA GCCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ATAGACAAGT ACAGAAAGAA
    CTAGGAGATG TCATTGTCCG TCTTCATACT CCAGTTGTGT TGACTCCCAC CACTGTTCAG
    GTCACATGGA CGGTTGATCG CCAGCCCCAG TTTATTCAAG GCTACCGAGT GATGTATCGT
    CAGACCACAG GCCTGCAGGC TACGACAACA TGGCAGAATT TAGATGCCAA AGTCCCAACC
    GAGCGAAGTG CTGTCTTGGT CAACCTGAAA AAGGGAGTGA CTTATGAAAT TAAAGTTCGA
    CCATATTTTA ATGAGTTCCA AGGAATGGAC AGTGAATCTA AAATAGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCTCC ACAATCTGTC ACTGTGCTGA CAGTTGGAAG CCACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ATCAGAATGG AATCATCCAA
    GAATACAAGA TCTGGTGTCT GGGGAATGAA ACACGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GATCTGTGAT AATTGGTGGA TTGTTCCCAG GTATTCAGTA CCGGGTAGAG
    GTTGCAGCTA GTACAAGTGC AGGGGTTGGA GTAAAAAGTG AGCCGCAGCC GATAATAATT
    GGGGGACGTA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTTGTGA AGCAACCAGC CTTTATAGCT GGAATTGGTG GTGCCTGCTG GGTAATTTTG
    ATGGGTTTTA GCATCTGGTT GTATTGGCGA AGAAAGAAGA GAAAGGGACT CAGTAACTAT
    GCTGTTACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCTGGTCTT
    CTAAATGCTG GTGATCCCAG TTACCCATGG CTTGCTGATT CATGGCCAGC CACGAGCTTA
    CCAGTAAACA ATAGCAATAG TGGCCCAAAT GAGATTGGGA ATTTTGGACG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC GACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACAAC ACAGATCTTG CATTCCAATA GCATACATGA ATTGGCTGTC
    GATCTGCCGG ACCCACAGTG GAAGAGCTCA ATTCAGCAAA AAACGGACCT GATGGGCTTT
    GGATATTCTC TCCCTGATCA GAACAAGGGT AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAGAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCGAC TTGGGCCAAT
    GTACCCCTAC CTCCTCCCCC AGTCCAGCCC CTTCCTGGTA CAGAGCTGGA ACACTATGCA
    GTGGAACCGC AAGAAAATGG GTATGACAGT GATAGCTGGT GCCCGCCATT ACCGGTACAA
    ACATACTTAC ACCAGGGTAT GGAAGATGAA CTGGAAGAAG ATGATGATCG TGTCCCAACA
    CCTCCTGTCC GAGGCGTGGC TTCTTCTCCT GCTATTTCCT TCGGACAGCA GTCCACTGCA
    ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAAGCTCA CCTGGATGAG
    TTGACAAGAG CCTATCAGTT TGATATAGCG AAGCAAACAT GGCACATTCA AAGCAATAAT
    CAACCTCCAC AGCCCCCGGT TCCACCATTA GGTTACATGT CCGGAGCCTT GATTTCCGAT
    TTGGAAACAG ATGTTCCAGA TGATGACGCT GATGATGAGG AGGAAGCTTT AGAAATCCCC
    AGACCCCTGA GAGCACTAGA GCAGACACCC GGATCCAGTA TGGACAATCT AGACAGCTCT
    GTGACAGGCA AAGCTTTTAC CTCCTCTCAA AGACCTCGAC CAACCAGCCC ATTTTCTACT
    GACAGTAATA CCAGTGCAGC CCTGAGTCAA AATCAGAGGC CTCGGCCCAC TAAAAAACAC
    AAGGGAGGGC GAATGGATCA ACAACCAGCA TTGCCTCATC GAAGGGAAGG AATGACAGAT
    GATCTTCCAC CACCACCAGA TCCCCCTCCA GGTCAGGGTT TAAGGCAGCA AATAGGCCCG
    AGCCAGCATG CTGGTAACAT GGAAAACTCA ACAGAGAGAA AAGGAAGCTC TCTGGAGACA
    CCACAAGCAT CCAACTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAGAACGTCC
    CTAGAGTGGC AGCGACAAAC CCAAGAATGG ATAAACTCCA CAGAACGCCA AGAAGATATA
    CGGAAAGCCC TACACAAACA AAGTGTTGGA CCAGAGGAAA CATTGGTGCC ATACAGCAAG
    CCCAGTTTCC CATCTCCGGG AGGTCACAGC TCATCAGGAA CAGCATCTTC TAAAGGATCC
    ACTGGACCTA GGAAAGCCGA GGCGTTGAGG GGAAGTCACC AACGCAATGC CAGTGACCTT
    CTTGACATAG GATATATGGG CTCAAATAGT CAAGGACAGT TTACAGGTGA ATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44449
    Clone ID Related Accession (Same CDS sequence) XM_014109730.1 , XM_014109730.2
    Accession Version XM_014109730.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4617bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product roundabout homolog 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109730.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTTACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAAAAAGA AGGCAGCCAG
    AACTTGCTTT TTCCAAATCA ACCCCAGCAA CCCAACAGTA GATGCTCTGT TTCGCCAACT
    GGAGACCTCA CAATTACCAA CATTCAGCGC TCCGATGCGG GTTACTATAT CTGCCAGGCA
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AAGTTACTGA TGTTTTAACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCTGCAAACC AAACTCTAGC AGTTGATGGT
    ACAGCATTAC TGAAATGTAA AGCTACTGGG GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCTGGG TAGAGATTCA AGAGCAACTA TACAAGAACA AGGAACATTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGTACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGGGAGA CTTCGTGGAG TGCTGTGCTG GATGTGACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ACTTAAGTGA CCTACCAGGG CCACCATCCA AACCACAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAA CCAGGTACCC CTGGAGCCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC GGTGGCAAAC
    CATGTAAAGA CCACGCTCTA TACAGTGAGA GGATTGCGGC CCAATACGAT CTATTTATTC
    ATGGTCAGAG CTATCAACCC CCAAGGTCTA AGCGACCCAA GCCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ATAGACAAGT ACAGAAAGAA
    CTAGGAGATG TCATTGTCCG TCTTCATACT CCAGTTGTGT TGACTCCCAC CACTGTTCAG
    GTCACATGGA CGGTTGATCG CCAGCCCCAG TTTATTCAAG GCTACCGAGT GATGTATCGT
    CAGACCACAG GCCTGCAGGC TACGACAACA TGGCAGAATT TAGATGCCAA AGTCCCAACC
    GAGCGAAGTG CTGTCTTGGT CAACCTGAAA AAGGGAGTGA CTTATGAAAT TAAAGTTCGA
    CCATATTTTA ATGAGTTCCA AGGAATGGAC AGTGAATCTA AAATAGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCTCC ACAATCTGTC ACTGTGCTGA CAGTTGGAAG CCACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ATCAGAATGG AATCATCCAA
    GAATACAAGA TCTGGTGTCT GGGGAATGAA ACACGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GATCTGTGAT AATTGGTGGA TTGTTCCCAG GTATTCAGTA CCGGGTAGAG
    GTTGCAGCTA GTACAAGTGC AGGGGTTGGA GTAAAAAGTG AGCCGCAGCC GATAATAATT
    GGGGGACGTA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTTGTGA AGCAACCAGC CTTTATAGCT GGAATTGGTG GTGCCTGCTG GGTAATTTTG
    ATGGGTTTTA GCATCTGGTT GTATTGGCGA AGAAAGAAGA GAAAGGGACT CAGTAACTAT
    GCTGTTACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCTGGTCTT
    CTAAATGCTG GTGATCCCAG TTACCCATGG CTTGCTGATT CATGGCCAGC CACGAGCTTA
    CCAGTAAACA ATAGCAATAG TGGCCCAAAT GAGATTGGGA ATTTTGGACG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC GACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACAAC ACAGATCTTG CATTCCAATA GCATACATGA ATTGGCTGTC
    GATCTGCCGG ACCCACAGTG GAAGAGCTCA ATTCAGCAAA AAACGGACCT GATGGGCTTT
    GGATATTCTC TCCCTGATCA GAACAAGGGT AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAGAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCGAC TTGGGCCAAT
    GTACCCCTAC CTCCTCCCCC AGTCCAGCCC CTTCCTGGTA CAGAGCTGGA ACACTATGCA
    GTGGAACCGC AAGAAAATGG GTATGACAGT GATAGCTGGT GCCCGCCATT ACCGGTACAA
    ACATACTTAC ACCAGGGTAT GGAAGATGAA CTGGAAGAAG ATGATGATCG TGTCCCAACA
    CCTCCTGTCC GAGGCGTGGC TTCTTCTCCT GCTATTTCCT TCGGACAGCA GTCCACTGCA
    ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAAGCTCA CCTGGATGAG
    TTGACAAGAG CCTATCAGTT TGATATAGCG AAGCAAACAT GGCACATTCA AAGCAATAAT
    CAACCTCCAC AGCCCCCGGT TCCACCATTA GGTTACATGT CCGGAGCCTT GATTTCCGAT
    TTGGAAACAG ATGTTCCAGA TGATGACGCT GATGATGAGG AGGAAGCTTT AGAAATCCCC
    AGACCCCTGA GAGCACTAGA GCAGACACCC GGATCCAGTA TGGACAATCT AGACAGCTCT
    GTGACAGGCA AAGCTTTTAC CTCCTCTCAA AGACCTCGAC CAACCAGCCC ATTTTCTACT
    GACAGTAATA CCAGTGCAGC CCTGAGTCAA AATCAGAGGC CTCGGCCCAC TAAAAAACAC
    AAGGGAGGGC GAATGGATCA ACAACCAGCA TTGCCTCATC GAAGGGAAGG AATGACAGAT
    GATCTTCCAC CACCACCAGA TCCCCCTCCA GGTCAGGGTT TAAGGCAGCA AATAGGCCCG
    AGCCAGCATG CTGGTAACAT GGAAAACTCA ACAGAGAGAA AAGGAAGCTC TCTGGAGACA
    CCACAAGCAT CCAACTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAGAACGTCC
    CTAGAGTGGC AGCGACAAAC CCAAGAATGG ATAAACTCCA CAGAACGCCA AGAAGATATA
    CGGAAAGCCC TACACAAACA AAGTGTTGGA CCAGAGGAAA CATTGGTGCC ATACAGCAAG
    CCCAGTTTCC CATCTCCGGG AGGTCACAGC TCATCAGGAA CAGCATCTTC TAAAGGATCC
    ACTGGACCTA GGAAAGCCGA GGCGTTGAGG GGAAGTCACC AACGCAATGC CAGTGACCTT
    CTTGACATAG GATATATGGG CTCAAATAGT CAAGGACAGT TTACAGGTGA ATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965205.1
    CDS385..5001
    Translation

    Target ORF information:

    RefSeq Version XM_014109730.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109730.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTTACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAAAAAGA AGGCAGCCAG
    AACTTGCTTT TTCCAAATCA ACCCCAGCAA CCCAACAGTA GATGCTCTGT TTCGCCAACT
    GGAGACCTCA CAATTACCAA CATTCAGCGC TCCGATGCGG GTTACTATAT CTGCCAGGCA
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AAGTTACTGA TGTTTTAACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCTGCAAACC AAACTCTAGC AGTTGATGGT
    ACAGCATTAC TGAAATGTAA AGCTACTGGG GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCTGGG TAGAGATTCA AGAGCAACTA TACAAGAACA AGGAACATTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGTACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGGGAGA CTTCGTGGAG TGCTGTGCTG GATGTGACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ACTTAAGTGA CCTACCAGGG CCACCATCCA AACCACAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAA CCAGGTACCC CTGGAGCCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC GGTGGCAAAC
    CATGTAAAGA CCACGCTCTA TACAGTGAGA GGATTGCGGC CCAATACGAT CTATTTATTC
    ATGGTCAGAG CTATCAACCC CCAAGGTCTA AGCGACCCAA GCCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ATAGACAAGT ACAGAAAGAA
    CTAGGAGATG TCATTGTCCG TCTTCATACT CCAGTTGTGT TGACTCCCAC CACTGTTCAG
    GTCACATGGA CGGTTGATCG CCAGCCCCAG TTTATTCAAG GCTACCGAGT GATGTATCGT
    CAGACCACAG GCCTGCAGGC TACGACAACA TGGCAGAATT TAGATGCCAA AGTCCCAACC
    GAGCGAAGTG CTGTCTTGGT CAACCTGAAA AAGGGAGTGA CTTATGAAAT TAAAGTTCGA
    CCATATTTTA ATGAGTTCCA AGGAATGGAC AGTGAATCTA AAATAGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCTCC ACAATCTGTC ACTGTGCTGA CAGTTGGAAG CCACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ATCAGAATGG AATCATCCAA
    GAATACAAGA TCTGGTGTCT GGGGAATGAA ACACGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GATCTGTGAT AATTGGTGGA TTGTTCCCAG GTATTCAGTA CCGGGTAGAG
    GTTGCAGCTA GTACAAGTGC AGGGGTTGGA GTAAAAAGTG AGCCGCAGCC GATAATAATT
    GGGGGACGTA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTTGTGA AGCAACCAGC CTTTATAGCT GGAATTGGTG GTGCCTGCTG GGTAATTTTG
    ATGGGTTTTA GCATCTGGTT GTATTGGCGA AGAAAGAAGA GAAAGGGACT CAGTAACTAT
    GCTGTTACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCTGGTCTT
    CTAAATGCTG GTGATCCCAG TTACCCATGG CTTGCTGATT CATGGCCAGC CACGAGCTTA
    CCAGTAAACA ATAGCAATAG TGGCCCAAAT GAGATTGGGA ATTTTGGACG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC GACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACAAC ACAGATCTTG CATTCCAATA GCATACATGA ATTGGCTGTC
    GATCTGCCGG ACCCACAGTG GAAGAGCTCA ATTCAGCAAA AAACGGACCT GATGGGCTTT
    GGATATTCTC TCCCTGATCA GAACAAGGGT AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAGAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCGAC TTGGGCCAAT
    GTACCCCTAC CTCCTCCCCC AGTCCAGCCC CTTCCTGGTA CAGAGCTGGA ACACTATGCA
    GTGGAACCGC AAGAAAATGG GTATGACAGT GATAGCTGGT GCCCGCCATT ACCGGTACAA
    ACATACTTAC ACCAGGGTAT GGAAGATGAA CTGGAAGAAG ATGATGATCG TGTCCCAACA
    CCTCCTGTCC GAGGCGTGGC TTCTTCTCCT GCTATTTCCT TCGGACAGCA GTCCACTGCA
    ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAAGCTCA CCTGGATGAG
    TTGACAAGAG CCTATCAGTT TGATATAGCG AAGCAAACAT GGCACATTCA AAGCAATAAT
    CAACCTCCAC AGCCCCCGGT TCCACCATTA GGTTACATGT CCGGAGCCTT GATTTCCGAT
    TTGGAAACAG ATGTTCCAGA TGATGACGCT GATGATGAGG AGGAAGCTTT AGAAATCCCC
    AGACCCCTGA GAGCACTAGA GCAGACACCC GGATCCAGTA TGGACAATCT AGACAGCTCT
    GTGACAGGCA AAGCTTTTAC CTCCTCTCAA AGACCTCGAC CAACCAGCCC ATTTTCTACT
    GACAGTAATA CCAGTGCAGC CCTGAGTCAA AATCAGAGGC CTCGGCCCAC TAAAAAACAC
    AAGGGAGGGC GAATGGATCA ACAACCAGCA TTGCCTCATC GAAGGGAAGG AATGACAGAT
    GATCTTCCAC CACCACCAGA TCCCCCTCCA GGTCAGGGTT TAAGGCAGCA AATAGGCCCG
    AGCCAGCATG CTGGTAACAT GGAAAACTCA ACAGAGAGAA AAGGAAGCTC TCTGGAGACA
    CCACAAGCAT CCAACTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAGAACGTCC
    CTAGAGTGGC AGCGACAAAC CCAAGAATGG ATAAACTCCA CAGAACGCCA AGAAGATATA
    CGGAAAGCCC TACACAAACA AAGTGTTGGA CCAGAGGAAA CATTGGTGCC ATACAGCAAG
    CCCAGTTTCC CATCTCCGGG AGGTCACAGC TCATCAGGAA CAGCATCTTC TAAAGGATCC
    ACTGGACCTA GGAAAGCCGA GGCGTTGAGG GGAAGTCACC AACGCAATGC CAGTGACCTT
    CTTGACATAG GATATATGGG CTCAAATAGT CAAGGACAGT TTACAGGTGA ATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44450
    Clone ID Related Accession (Same CDS sequence) XM_014109732.1 , XM_014109732.2
    Accession Version XM_014109732.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product roundabout homolog 2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCCTCC ACAGTTTGTG
    GTTAGGCCAA GAGATCAGAT TGTTGCTCAA GGTCGAACAG TTACATTTCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAAAAAGAAG GCAGCCAGAA CTTGCTTTTT
    CCAAATCAAC CCCAGCAACC CAACAGTAGA TGCTCTGTTT CGCCAACTGG AGACCTCACA
    ATTACCAACA TTCAGCGCTC CGATGCGGGT TACTATATCT GCCAGGCATT AACTGTGGCA
    GGAAGCATTT TAGCAAAAGC TCAACTGGAA GTTACTGATG TTTTAACAGA TAGACCTCCA
    CCTATAATTC TACAAGGCCC TGCAAACCAA ACTCTAGCAG TTGATGGTAC AGCATTACTG
    AAATGTAAAG CTACTGGGGA TCCTCTTCCT GTAATTAGCT GGTTAAAGGA GGGATTTACT
    TTTCTGGGTA GAGATTCAAG AGCAACTATA CAAGAACAAG GAACATTGCA GATTAAGAAT
    TTACGGATTT CTGATACTGG TACTTATACT TGTGTGGCTA CAAGTTCAAG TGGGGAGACT
    TCGTGGAGTG CTGTGCTGGA TGTGACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAC
    TTAAGTGACC TACCAGGGCC ACCATCCAAA CCACAGGTCA CTGATGTTAC TAAGAACAGT
    GTCACCTTGT CCTGGCAACC AGGTACCCCT GGAGCCCTTC CAGCAAGTGC ATATATCATT
    GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACGG TGGCAAACCA TGTAAAGACC
    ACGCTCTATA CAGTGAGAGG ATTGCGGCCC AATACGATCT ATTTATTCAT GGTCAGAGCT
    ATCAACCCCC AAGGTCTAAG CGACCCAAGC CCCATGTCAG ATCCTGTGCG CACACAAGAT
    ATCAGCCCAC CAGCACAAGG AGTGGACCAT AGACAAGTAC AGAAAGAACT AGGAGATGTC
    ATTGTCCGTC TTCATACTCC AGTTGTGTTG ACTCCCACCA CTGTTCAGGT CACATGGACG
    GTTGATCGCC AGCCCCAGTT TATTCAAGGC TACCGAGTGA TGTATCGTCA GACCACAGGC
    CTGCAGGCTA CGACAACATG GCAGAATTTA GATGCCAAAG TCCCAACCGA GCGAAGTGCT
    GTCTTGGTCA ACCTGAAAAA GGGAGTGACT TATGAAATTA AAGTTCGACC ATATTTTAAT
    GAGTTCCAAG GAATGGACAG TGAATCTAAA ATAGTTCGTA CTACTGAAGA AGCCCCAAGT
    GCCCCTCCAC AATCTGTCAC TGTGCTGACA GTTGGAAGCC ACAATAGCAC AAGTATTAGT
    GTTTCCTGGG ATCCTCCTCC TCCAGATCAT CAGAATGGAA TCATCCAAGA ATACAAGATC
    TGGTGTCTGG GGAATGAAAC ACGATTCCAT ATCAACAAAA CTGTGGATGC AGCCATTCGA
    TCTGTGATAA TTGGTGGATT GTTCCCAGGT ATTCAGTACC GGGTAGAGGT TGCAGCTAGT
    ACAAGTGCAG GGGTTGGAGT AAAAAGTGAG CCGCAGCCGA TAATAATTGG GGGACGTAAT
    GAAGTTGTCA TTACTGAAAA CAATAACAGC ATAACTGAGC AAATCACTGA TGTTGTGAAG
    CAACCAGCCT TTATAGCTGG AATTGGTGGT GCCTGCTGGG TAATTTTGAT GGGTTTTAGC
    ATCTGGTTGT ATTGGCGAAG AAAGAAGAGA AAGGGACTCA GTAACTATGC TGTTACATTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CTGGTCTTCT AAATGCTGGT
    GATCCCAGTT ACCCATGGCT TGCTGATTCA TGGCCAGCCA CGAGCTTACC AGTAAACAAT
    AGCAATAGTG GCCCAAATGA GATTGGGAAT TTTGGACGTG GAGATGTGCT GCCACCAGTT
    CCAGGCCAAG GGGATAAAAC AGCAACGATG CTCTCAGATG GAGCCATTTA TAGCAGCATT
    GACTTCACGA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC TACCCCATAT
    GCCACAACAC AGATCTTGCA TTCCAATAGC ATACATGAAT TGGCTGTCGA TCTGCCGGAC
    CCACAGTGGA AGAGCTCAAT TCAGCAAAAA ACGGACCTGA TGGGCTTTGG ATATTCTCTC
    CCTGATCAGA ACAAGGGTAA CAACGCCTTA CTTTACATCC CTGACTACCG ATTGGCTGAG
    GGATTGTCTA ATAGAATGCC ACACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC
    AACAGCTCAG AGAGGAGTGG CAGTCTCTCA GGTGGGAAAG GTGGAAAAAA GAAGAAAACT
    AAAAACTCTT CTAAACCACA GAAAAACAAT GGATCGACTT GGGCCAATGT ACCCCTACCT
    CCTCCCCCAG TCCAGCCCCT TCCTGGTACA GAGCTGGAAC ACTATGCAGT GGAACCGCAA
    GAAAATGGGT ATGACAGTGA TAGCTGGTGC CCGCCATTAC CGGTACAAAC ATACTTACAC
    CAGGGTATGG AAGATGAACT GGAAGAAGAT GATGATCGTG TCCCAACACC TCCTGTCCGA
    GGCGTGGCTT CTTCTCCTGC TATTTCCTTC GGACAGCAGT CCACTGCAAC TCTTACTCCA
    TCCCCACGGG AAGAGATGCA ACCCATGCTG CAAGCTCACC TGGATGAGTT GACAAGAGCC
    TATCAGTTTG ATATAGCGAA GCAAACATGG CACATTCAAA GCAATAATCA ACCTCCACAG
    CCCCCGGTTC CACCATTAGG TTACATGTCC GGAGCCTTGA TTTCCGATTT GGAAACAGAT
    GTTCCAGATG ATGACGCTGA TGATGAGGAG GAAGCTTTAG AAATCCCCAG ACCCCTGAGA
    GCACTAGAGC AGACACCCGG ATCCAGTATG GACAATCTAG ACAGCTCTGT GACAGGCAAA
    GCTTTTACCT CCTCTCAAAG ACCTCGACCA ACCAGCCCAT TTTCTACTGA CAGTAATACC
    AGTGCAGCCC TGAGTCAAAA TCAGAGGCCT CGGCCCACTA AAAAACACAA GGGAGGGCGA
    ATGGATCAAC AACCAGCATT GCCTCATCGA AGGGAAGGAA TGACAGATGA TCTTCCACCA
    CCACCAGATC CCCCTCCAGG TCAGGGTTTA AGGCAGCAAA TAGGCCCGAG CCAGCATGCT
    GGTAACATGG AAAACTCAAC AGAGAGAAAA GGAAGCTCTC TGGAGACACC ACAAGCATCC
    AACTTAGAAG ACACAAAGAG CTCATTGGAT TGTCCAGCTA GAACGTCCCT AGAGTGGCAG
    CGACAAACCC AAGAATGGAT AAACTCCACA GAACGCCAAG AAGATATACG GAAAGCCCTA
    CACAAACAAA GTGTTGGACC AGAGGAAACA TTGGTGCCAT ACAGCAAGCC CAGTTTCCCA
    TCTCCGGGAG GTCACAGCTC ATCAGGAACA GCATCTTCTA AAGGATCCAC TGGACCTAGG
    AAAGCCGAGG CGTTGAGGGG AAGTCACCAA CGCAATGCCA GTGACCTTCT TGACATAGGA
    TATATGGGCT CAAATAGTCA AGGACAGTTT ACAGGTGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965207.1
    CDS585..5189
    Translation

    Target ORF information:

    RefSeq Version XM_014109732.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109732.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCCTCC ACAGTTTGTG
    GTTAGGCCAA GAGATCAGAT TGTTGCTCAA GGTCGAACAG TTACATTTCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAAAAAGAAG GCAGCCAGAA CTTGCTTTTT
    CCAAATCAAC CCCAGCAACC CAACAGTAGA TGCTCTGTTT CGCCAACTGG AGACCTCACA
    ATTACCAACA TTCAGCGCTC CGATGCGGGT TACTATATCT GCCAGGCATT AACTGTGGCA
    GGAAGCATTT TAGCAAAAGC TCAACTGGAA GTTACTGATG TTTTAACAGA TAGACCTCCA
    CCTATAATTC TACAAGGCCC TGCAAACCAA ACTCTAGCAG TTGATGGTAC AGCATTACTG
    AAATGTAAAG CTACTGGGGA TCCTCTTCCT GTAATTAGCT GGTTAAAGGA GGGATTTACT
    TTTCTGGGTA GAGATTCAAG AGCAACTATA CAAGAACAAG GAACATTGCA GATTAAGAAT
    TTACGGATTT CTGATACTGG TACTTATACT TGTGTGGCTA CAAGTTCAAG TGGGGAGACT
    TCGTGGAGTG CTGTGCTGGA TGTGACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAC
    TTAAGTGACC TACCAGGGCC ACCATCCAAA CCACAGGTCA CTGATGTTAC TAAGAACAGT
    GTCACCTTGT CCTGGCAACC AGGTACCCCT GGAGCCCTTC CAGCAAGTGC ATATATCATT
    GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACGG TGGCAAACCA TGTAAAGACC
    ACGCTCTATA CAGTGAGAGG ATTGCGGCCC AATACGATCT ATTTATTCAT GGTCAGAGCT
    ATCAACCCCC AAGGTCTAAG CGACCCAAGC CCCATGTCAG ATCCTGTGCG CACACAAGAT
    ATCAGCCCAC CAGCACAAGG AGTGGACCAT AGACAAGTAC AGAAAGAACT AGGAGATGTC
    ATTGTCCGTC TTCATACTCC AGTTGTGTTG ACTCCCACCA CTGTTCAGGT CACATGGACG
    GTTGATCGCC AGCCCCAGTT TATTCAAGGC TACCGAGTGA TGTATCGTCA GACCACAGGC
    CTGCAGGCTA CGACAACATG GCAGAATTTA GATGCCAAAG TCCCAACCGA GCGAAGTGCT
    GTCTTGGTCA ACCTGAAAAA GGGAGTGACT TATGAAATTA AAGTTCGACC ATATTTTAAT
    GAGTTCCAAG GAATGGACAG TGAATCTAAA ATAGTTCGTA CTACTGAAGA AGCCCCAAGT
    GCCCCTCCAC AATCTGTCAC TGTGCTGACA GTTGGAAGCC ACAATAGCAC AAGTATTAGT
    GTTTCCTGGG ATCCTCCTCC TCCAGATCAT CAGAATGGAA TCATCCAAGA ATACAAGATC
    TGGTGTCTGG GGAATGAAAC ACGATTCCAT ATCAACAAAA CTGTGGATGC AGCCATTCGA
    TCTGTGATAA TTGGTGGATT GTTCCCAGGT ATTCAGTACC GGGTAGAGGT TGCAGCTAGT
    ACAAGTGCAG GGGTTGGAGT AAAAAGTGAG CCGCAGCCGA TAATAATTGG GGGACGTAAT
    GAAGTTGTCA TTACTGAAAA CAATAACAGC ATAACTGAGC AAATCACTGA TGTTGTGAAG
    CAACCAGCCT TTATAGCTGG AATTGGTGGT GCCTGCTGGG TAATTTTGAT GGGTTTTAGC
    ATCTGGTTGT ATTGGCGAAG AAAGAAGAGA AAGGGACTCA GTAACTATGC TGTTACATTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CTGGTCTTCT AAATGCTGGT
    GATCCCAGTT ACCCATGGCT TGCTGATTCA TGGCCAGCCA CGAGCTTACC AGTAAACAAT
    AGCAATAGTG GCCCAAATGA GATTGGGAAT TTTGGACGTG GAGATGTGCT GCCACCAGTT
    CCAGGCCAAG GGGATAAAAC AGCAACGATG CTCTCAGATG GAGCCATTTA TAGCAGCATT
    GACTTCACGA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC TACCCCATAT
    GCCACAACAC AGATCTTGCA TTCCAATAGC ATACATGAAT TGGCTGTCGA TCTGCCGGAC
    CCACAGTGGA AGAGCTCAAT TCAGCAAAAA ACGGACCTGA TGGGCTTTGG ATATTCTCTC
    CCTGATCAGA ACAAGGGTAA CAACGCCTTA CTTTACATCC CTGACTACCG ATTGGCTGAG
    GGATTGTCTA ATAGAATGCC ACACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC
    AACAGCTCAG AGAGGAGTGG CAGTCTCTCA GGTGGGAAAG GTGGAAAAAA GAAGAAAACT
    AAAAACTCTT CTAAACCACA GAAAAACAAT GGATCGACTT GGGCCAATGT ACCCCTACCT
    CCTCCCCCAG TCCAGCCCCT TCCTGGTACA GAGCTGGAAC ACTATGCAGT GGAACCGCAA
    GAAAATGGGT ATGACAGTGA TAGCTGGTGC CCGCCATTAC CGGTACAAAC ATACTTACAC
    CAGGGTATGG AAGATGAACT GGAAGAAGAT GATGATCGTG TCCCAACACC TCCTGTCCGA
    GGCGTGGCTT CTTCTCCTGC TATTTCCTTC GGACAGCAGT CCACTGCAAC TCTTACTCCA
    TCCCCACGGG AAGAGATGCA ACCCATGCTG CAAGCTCACC TGGATGAGTT GACAAGAGCC
    TATCAGTTTG ATATAGCGAA GCAAACATGG CACATTCAAA GCAATAATCA ACCTCCACAG
    CCCCCGGTTC CACCATTAGG TTACATGTCC GGAGCCTTGA TTTCCGATTT GGAAACAGAT
    GTTCCAGATG ATGACGCTGA TGATGAGGAG GAAGCTTTAG AAATCCCCAG ACCCCTGAGA
    GCACTAGAGC AGACACCCGG ATCCAGTATG GACAATCTAG ACAGCTCTGT GACAGGCAAA
    GCTTTTACCT CCTCTCAAAG ACCTCGACCA ACCAGCCCAT TTTCTACTGA CAGTAATACC
    AGTGCAGCCC TGAGTCAAAA TCAGAGGCCT CGGCCCACTA AAAAACACAA GGGAGGGCGA
    ATGGATCAAC AACCAGCATT GCCTCATCGA AGGGAAGGAA TGACAGATGA TCTTCCACCA
    CCACCAGATC CCCCTCCAGG TCAGGGTTTA AGGCAGCAAA TAGGCCCGAG CCAGCATGCT
    GGTAACATGG AAAACTCAAC AGAGAGAAAA GGAAGCTCTC TGGAGACACC ACAAGCATCC
    AACTTAGAAG ACACAAAGAG CTCATTGGAT TGTCCAGCTA GAACGTCCCT AGAGTGGCAG
    CGACAAACCC AAGAATGGAT AAACTCCACA GAACGCCAAG AAGATATACG GAAAGCCCTA
    CACAAACAAA GTGTTGGACC AGAGGAAACA TTGGTGCCAT ACAGCAAGCC CAGTTTCCCA
    TCTCCGGGAG GTCACAGCTC ATCAGGAACA GCATCTTCTA AAGGATCCAC TGGACCTAGG
    AAAGCCGAGG CGTTGAGGGG AAGTCACCAA CGCAATGCCA GTGACCTTCT TGACATAGGA
    TATATGGGCT CAAATAGTCA AGGACAGTTT ACAGGTGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44450
    Clone ID Related Accession (Same CDS sequence) XM_014109732.1 , XM_014109732.2
    Accession Version XM_014109732.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product roundabout homolog 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109732.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCCTCC ACAGTTTGTG
    GTTAGGCCAA GAGATCAGAT TGTTGCTCAA GGTCGAACAG TTACATTTCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAAAAAGAAG GCAGCCAGAA CTTGCTTTTT
    CCAAATCAAC CCCAGCAACC CAACAGTAGA TGCTCTGTTT CGCCAACTGG AGACCTCACA
    ATTACCAACA TTCAGCGCTC CGATGCGGGT TACTATATCT GCCAGGCATT AACTGTGGCA
    GGAAGCATTT TAGCAAAAGC TCAACTGGAA GTTACTGATG TTTTAACAGA TAGACCTCCA
    CCTATAATTC TACAAGGCCC TGCAAACCAA ACTCTAGCAG TTGATGGTAC AGCATTACTG
    AAATGTAAAG CTACTGGGGA TCCTCTTCCT GTAATTAGCT GGTTAAAGGA GGGATTTACT
    TTTCTGGGTA GAGATTCAAG AGCAACTATA CAAGAACAAG GAACATTGCA GATTAAGAAT
    TTACGGATTT CTGATACTGG TACTTATACT TGTGTGGCTA CAAGTTCAAG TGGGGAGACT
    TCGTGGAGTG CTGTGCTGGA TGTGACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAC
    TTAAGTGACC TACCAGGGCC ACCATCCAAA CCACAGGTCA CTGATGTTAC TAAGAACAGT
    GTCACCTTGT CCTGGCAACC AGGTACCCCT GGAGCCCTTC CAGCAAGTGC ATATATCATT
    GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACGG TGGCAAACCA TGTAAAGACC
    ACGCTCTATA CAGTGAGAGG ATTGCGGCCC AATACGATCT ATTTATTCAT GGTCAGAGCT
    ATCAACCCCC AAGGTCTAAG CGACCCAAGC CCCATGTCAG ATCCTGTGCG CACACAAGAT
    ATCAGCCCAC CAGCACAAGG AGTGGACCAT AGACAAGTAC AGAAAGAACT AGGAGATGTC
    ATTGTCCGTC TTCATACTCC AGTTGTGTTG ACTCCCACCA CTGTTCAGGT CACATGGACG
    GTTGATCGCC AGCCCCAGTT TATTCAAGGC TACCGAGTGA TGTATCGTCA GACCACAGGC
    CTGCAGGCTA CGACAACATG GCAGAATTTA GATGCCAAAG TCCCAACCGA GCGAAGTGCT
    GTCTTGGTCA ACCTGAAAAA GGGAGTGACT TATGAAATTA AAGTTCGACC ATATTTTAAT
    GAGTTCCAAG GAATGGACAG TGAATCTAAA ATAGTTCGTA CTACTGAAGA AGCCCCAAGT
    GCCCCTCCAC AATCTGTCAC TGTGCTGACA GTTGGAAGCC ACAATAGCAC AAGTATTAGT
    GTTTCCTGGG ATCCTCCTCC TCCAGATCAT CAGAATGGAA TCATCCAAGA ATACAAGATC
    TGGTGTCTGG GGAATGAAAC ACGATTCCAT ATCAACAAAA CTGTGGATGC AGCCATTCGA
    TCTGTGATAA TTGGTGGATT GTTCCCAGGT ATTCAGTACC GGGTAGAGGT TGCAGCTAGT
    ACAAGTGCAG GGGTTGGAGT AAAAAGTGAG CCGCAGCCGA TAATAATTGG GGGACGTAAT
    GAAGTTGTCA TTACTGAAAA CAATAACAGC ATAACTGAGC AAATCACTGA TGTTGTGAAG
    CAACCAGCCT TTATAGCTGG AATTGGTGGT GCCTGCTGGG TAATTTTGAT GGGTTTTAGC
    ATCTGGTTGT ATTGGCGAAG AAAGAAGAGA AAGGGACTCA GTAACTATGC TGTTACATTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CTGGTCTTCT AAATGCTGGT
    GATCCCAGTT ACCCATGGCT TGCTGATTCA TGGCCAGCCA CGAGCTTACC AGTAAACAAT
    AGCAATAGTG GCCCAAATGA GATTGGGAAT TTTGGACGTG GAGATGTGCT GCCACCAGTT
    CCAGGCCAAG GGGATAAAAC AGCAACGATG CTCTCAGATG GAGCCATTTA TAGCAGCATT
    GACTTCACGA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC TACCCCATAT
    GCCACAACAC AGATCTTGCA TTCCAATAGC ATACATGAAT TGGCTGTCGA TCTGCCGGAC
    CCACAGTGGA AGAGCTCAAT TCAGCAAAAA ACGGACCTGA TGGGCTTTGG ATATTCTCTC
    CCTGATCAGA ACAAGGGTAA CAACGCCTTA CTTTACATCC CTGACTACCG ATTGGCTGAG
    GGATTGTCTA ATAGAATGCC ACACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC
    AACAGCTCAG AGAGGAGTGG CAGTCTCTCA GGTGGGAAAG GTGGAAAAAA GAAGAAAACT
    AAAAACTCTT CTAAACCACA GAAAAACAAT GGATCGACTT GGGCCAATGT ACCCCTACCT
    CCTCCCCCAG TCCAGCCCCT TCCTGGTACA GAGCTGGAAC ACTATGCAGT GGAACCGCAA
    GAAAATGGGT ATGACAGTGA TAGCTGGTGC CCGCCATTAC CGGTACAAAC ATACTTACAC
    CAGGGTATGG AAGATGAACT GGAAGAAGAT GATGATCGTG TCCCAACACC TCCTGTCCGA
    GGCGTGGCTT CTTCTCCTGC TATTTCCTTC GGACAGCAGT CCACTGCAAC TCTTACTCCA
    TCCCCACGGG AAGAGATGCA ACCCATGCTG CAAGCTCACC TGGATGAGTT GACAAGAGCC
    TATCAGTTTG ATATAGCGAA GCAAACATGG CACATTCAAA GCAATAATCA ACCTCCACAG
    CCCCCGGTTC CACCATTAGG TTACATGTCC GGAGCCTTGA TTTCCGATTT GGAAACAGAT
    GTTCCAGATG ATGACGCTGA TGATGAGGAG GAAGCTTTAG AAATCCCCAG ACCCCTGAGA
    GCACTAGAGC AGACACCCGG ATCCAGTATG GACAATCTAG ACAGCTCTGT GACAGGCAAA
    GCTTTTACCT CCTCTCAAAG ACCTCGACCA ACCAGCCCAT TTTCTACTGA CAGTAATACC
    AGTGCAGCCC TGAGTCAAAA TCAGAGGCCT CGGCCCACTA AAAAACACAA GGGAGGGCGA
    ATGGATCAAC AACCAGCATT GCCTCATCGA AGGGAAGGAA TGACAGATGA TCTTCCACCA
    CCACCAGATC CCCCTCCAGG TCAGGGTTTA AGGCAGCAAA TAGGCCCGAG CCAGCATGCT
    GGTAACATGG AAAACTCAAC AGAGAGAAAA GGAAGCTCTC TGGAGACACC ACAAGCATCC
    AACTTAGAAG ACACAAAGAG CTCATTGGAT TGTCCAGCTA GAACGTCCCT AGAGTGGCAG
    CGACAAACCC AAGAATGGAT AAACTCCACA GAACGCCAAG AAGATATACG GAAAGCCCTA
    CACAAACAAA GTGTTGGACC AGAGGAAACA TTGGTGCCAT ACAGCAAGCC CAGTTTCCCA
    TCTCCGGGAG GTCACAGCTC ATCAGGAACA GCATCTTCTA AAGGATCCAC TGGACCTAGG
    AAAGCCGAGG CGTTGAGGGG AAGTCACCAA CGCAATGCCA GTGACCTTCT TGACATAGGA
    TATATGGGCT CAAATAGTCA AGGACAGTTT ACAGGTGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965207.1
    CDS386..4990
    Translation

    Target ORF information:

    RefSeq Version XM_014109732.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109732.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCCTCC ACAGTTTGTG
    GTTAGGCCAA GAGATCAGAT TGTTGCTCAA GGTCGAACAG TTACATTTCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAAAAAGAAG GCAGCCAGAA CTTGCTTTTT
    CCAAATCAAC CCCAGCAACC CAACAGTAGA TGCTCTGTTT CGCCAACTGG AGACCTCACA
    ATTACCAACA TTCAGCGCTC CGATGCGGGT TACTATATCT GCCAGGCATT AACTGTGGCA
    GGAAGCATTT TAGCAAAAGC TCAACTGGAA GTTACTGATG TTTTAACAGA TAGACCTCCA
    CCTATAATTC TACAAGGCCC TGCAAACCAA ACTCTAGCAG TTGATGGTAC AGCATTACTG
    AAATGTAAAG CTACTGGGGA TCCTCTTCCT GTAATTAGCT GGTTAAAGGA GGGATTTACT
    TTTCTGGGTA GAGATTCAAG AGCAACTATA CAAGAACAAG GAACATTGCA GATTAAGAAT
    TTACGGATTT CTGATACTGG TACTTATACT TGTGTGGCTA CAAGTTCAAG TGGGGAGACT
    TCGTGGAGTG CTGTGCTGGA TGTGACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAC
    TTAAGTGACC TACCAGGGCC ACCATCCAAA CCACAGGTCA CTGATGTTAC TAAGAACAGT
    GTCACCTTGT CCTGGCAACC AGGTACCCCT GGAGCCCTTC CAGCAAGTGC ATATATCATT
    GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACGG TGGCAAACCA TGTAAAGACC
    ACGCTCTATA CAGTGAGAGG ATTGCGGCCC AATACGATCT ATTTATTCAT GGTCAGAGCT
    ATCAACCCCC AAGGTCTAAG CGACCCAAGC CCCATGTCAG ATCCTGTGCG CACACAAGAT
    ATCAGCCCAC CAGCACAAGG AGTGGACCAT AGACAAGTAC AGAAAGAACT AGGAGATGTC
    ATTGTCCGTC TTCATACTCC AGTTGTGTTG ACTCCCACCA CTGTTCAGGT CACATGGACG
    GTTGATCGCC AGCCCCAGTT TATTCAAGGC TACCGAGTGA TGTATCGTCA GACCACAGGC
    CTGCAGGCTA CGACAACATG GCAGAATTTA GATGCCAAAG TCCCAACCGA GCGAAGTGCT
    GTCTTGGTCA ACCTGAAAAA GGGAGTGACT TATGAAATTA AAGTTCGACC ATATTTTAAT
    GAGTTCCAAG GAATGGACAG TGAATCTAAA ATAGTTCGTA CTACTGAAGA AGCCCCAAGT
    GCCCCTCCAC AATCTGTCAC TGTGCTGACA GTTGGAAGCC ACAATAGCAC AAGTATTAGT
    GTTTCCTGGG ATCCTCCTCC TCCAGATCAT CAGAATGGAA TCATCCAAGA ATACAAGATC
    TGGTGTCTGG GGAATGAAAC ACGATTCCAT ATCAACAAAA CTGTGGATGC AGCCATTCGA
    TCTGTGATAA TTGGTGGATT GTTCCCAGGT ATTCAGTACC GGGTAGAGGT TGCAGCTAGT
    ACAAGTGCAG GGGTTGGAGT AAAAAGTGAG CCGCAGCCGA TAATAATTGG GGGACGTAAT
    GAAGTTGTCA TTACTGAAAA CAATAACAGC ATAACTGAGC AAATCACTGA TGTTGTGAAG
    CAACCAGCCT TTATAGCTGG AATTGGTGGT GCCTGCTGGG TAATTTTGAT GGGTTTTAGC
    ATCTGGTTGT ATTGGCGAAG AAAGAAGAGA AAGGGACTCA GTAACTATGC TGTTACATTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CTGGTCTTCT AAATGCTGGT
    GATCCCAGTT ACCCATGGCT TGCTGATTCA TGGCCAGCCA CGAGCTTACC AGTAAACAAT
    AGCAATAGTG GCCCAAATGA GATTGGGAAT TTTGGACGTG GAGATGTGCT GCCACCAGTT
    CCAGGCCAAG GGGATAAAAC AGCAACGATG CTCTCAGATG GAGCCATTTA TAGCAGCATT
    GACTTCACGA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC TACCCCATAT
    GCCACAACAC AGATCTTGCA TTCCAATAGC ATACATGAAT TGGCTGTCGA TCTGCCGGAC
    CCACAGTGGA AGAGCTCAAT TCAGCAAAAA ACGGACCTGA TGGGCTTTGG ATATTCTCTC
    CCTGATCAGA ACAAGGGTAA CAACGCCTTA CTTTACATCC CTGACTACCG ATTGGCTGAG
    GGATTGTCTA ATAGAATGCC ACACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC
    AACAGCTCAG AGAGGAGTGG CAGTCTCTCA GGTGGGAAAG GTGGAAAAAA GAAGAAAACT
    AAAAACTCTT CTAAACCACA GAAAAACAAT GGATCGACTT GGGCCAATGT ACCCCTACCT
    CCTCCCCCAG TCCAGCCCCT TCCTGGTACA GAGCTGGAAC ACTATGCAGT GGAACCGCAA
    GAAAATGGGT ATGACAGTGA TAGCTGGTGC CCGCCATTAC CGGTACAAAC ATACTTACAC
    CAGGGTATGG AAGATGAACT GGAAGAAGAT GATGATCGTG TCCCAACACC TCCTGTCCGA
    GGCGTGGCTT CTTCTCCTGC TATTTCCTTC GGACAGCAGT CCACTGCAAC TCTTACTCCA
    TCCCCACGGG AAGAGATGCA ACCCATGCTG CAAGCTCACC TGGATGAGTT GACAAGAGCC
    TATCAGTTTG ATATAGCGAA GCAAACATGG CACATTCAAA GCAATAATCA ACCTCCACAG
    CCCCCGGTTC CACCATTAGG TTACATGTCC GGAGCCTTGA TTTCCGATTT GGAAACAGAT
    GTTCCAGATG ATGACGCTGA TGATGAGGAG GAAGCTTTAG AAATCCCCAG ACCCCTGAGA
    GCACTAGAGC AGACACCCGG ATCCAGTATG GACAATCTAG ACAGCTCTGT GACAGGCAAA
    GCTTTTACCT CCTCTCAAAG ACCTCGACCA ACCAGCCCAT TTTCTACTGA CAGTAATACC
    AGTGCAGCCC TGAGTCAAAA TCAGAGGCCT CGGCCCACTA AAAAACACAA GGGAGGGCGA
    ATGGATCAAC AACCAGCATT GCCTCATCGA AGGGAAGGAA TGACAGATGA TCTTCCACCA
    CCACCAGATC CCCCTCCAGG TCAGGGTTTA AGGCAGCAAA TAGGCCCGAG CCAGCATGCT
    GGTAACATGG AAAACTCAAC AGAGAGAAAA GGAAGCTCTC TGGAGACACC ACAAGCATCC
    AACTTAGAAG ACACAAAGAG CTCATTGGAT TGTCCAGCTA GAACGTCCCT AGAGTGGCAG
    CGACAAACCC AAGAATGGAT AAACTCCACA GAACGCCAAG AAGATATACG GAAAGCCCTA
    CACAAACAAA GTGTTGGACC AGAGGAAACA TTGGTGCCAT ACAGCAAGCC CAGTTTCCCA
    TCTCCGGGAG GTCACAGCTC ATCAGGAACA GCATCTTCTA AAGGATCCAC TGGACCTAGG
    AAAGCCGAGG CGTTGAGGGG AAGTCACCAA CGCAATGCCA GTGACCTTCT TGACATAGGA
    TATATGGGCT CAAATAGTCA AGGACAGTTT ACAGGTGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44451
    Clone ID Related Accession (Same CDS sequence) XM_014109733.1
    Accession Version XM_014109733.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4596bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product roundabout homolog 2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGACCAGAA GACAAGGAAG TGTTACTAGA AGAATGTGGA CATGGACTCC GGAACTGCTA 
    ATGGTGACTG TGATCCTCTG GGGTCAGCAA GGAAATGGAC AAGGCCAAGG ATCTCGTCTT
    CGCCAAGAAG ACTTCCCTCC GCGGATTGTG GAGCACCCTT CAGATGTCAT TGTCTCTAAG
    GGAGAGCCCA CCACCTTGAA CTGTAAGGCA GAGGGCCGGC CAACGCCCAC CATTGAGTGG
    TACAAGGATG GCGAGCGGGT GGAGACTGAC AAGGATGATC CCCGGTCTCA CAGGATGCTT
    CTGCCCAGCG GATCCTTATT TTTCTTGCGC ATTGTTCATG GGCGCAGAAG CAAACCCGAT
    GAAGGAAGCT ACGTCTGTGT TGCGAGGAAC TATCTCGGTG AAGCAGTAAG TCGAAATGCA
    TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGGCAAA ATCCCACAGA TGTTGTAGTG
    GCAGCCGGAG AGCCTGCAAT CCTGGAGTGC CAACCTCCCA GGGGACACCC AGAACCCACG
    ATCTACTGGA AAAAAGACAA AGTCCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT
    GGTGGAAAGC TGATGATATC CAATACCAGG AAAAGTGATG CAGGGATGTA TACCTGTGTT
    GGCACCAATA TGGTGGGAGA AAGAGACAGT GATCCAGCAG AGCTGACTGT CTTTGAACGA
    CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AAGAAGAAGC TGTAGAATTT
    CGTTGTCAGG TCCAAGGAGA TCCTCAACCA ACTGTGCGGT GGAAAAAGGA TGATGCAGAC
    CTGCCAAGAG GAAGGTATGA CATCAAAGAT GATTACACAC TGAGAATTAA AAAGGCCATG
    AGTACAGATG AAGGCACCTA TCTGTGTATT GCCGAGAATC GGGTTGGAAA AGTAGAAGCC
    TCTGCTTCTC TAACAGTCCG AGCTCGCCCT GTTGCTCCTC CACAGTTTGT GGTTAGGCCA
    AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTTACATTTC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAAAAAGAA GGCAGCCAGA ACTTGCTTTT TCCAAATCAA
    CCCCAGCAAC CCAACAGTAG ATGCTCTGTT TCGCCAACTG GAGACCTCAC AATTACCAAC
    ATTCAGCGCT CCGATGCGGG TTACTATATC TGCCAGGCAT TAACTGTGGC AGGAAGCATT
    TTAGCAAAAG CTCAACTGGA AGTTACTGAT GTTTTAACAG ATAGACCTCC ACCTATAATT
    CTACAAGGCC CTGCAAACCA AACTCTAGCA GTTGATGGTA CAGCATTACT GAAATGTAAA
    GCTACTGGGG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCTGGGT
    AGAGATTCAA GAGCAACTAT ACAAGAACAA GGAACATTGC AGATTAAGAA TTTACGGATT
    TCTGATACTG GTACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGGGAGAC TTCGTGGAGT
    GCTGTGCTGG ATGTGACAGA ATCTGGAGCA ACAATCAGTA AAAATTATGA CTTAAGTGAC
    CTACCAGGGC CACCATCCAA ACCACAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG
    TCCTGGCAAC CAGGTACCCC TGGAGCCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC
    AGCCAATCGG TGAGCAATAG CTGGCAGACG GTGGCAAACC ATGTAAAGAC CACGCTCTAT
    ACAGTGAGAG GATTGCGGCC CAATACGATC TATTTATTCA TGGTCAGAGC TATCAACCCC
    CAAGGTCTAA GCGACCCAAG CCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA
    CCAGCACAAG GAGTGGACCA TAGACAAGTA CAGAAAGAAC TAGGAGATGT CATTGTCCGT
    CTTCATACTC CAGTTGTGTT GACTCCCACC ACTGTTCAGG TCACATGGAC GGTTGATCGC
    CAGCCCCAGT TTATTCAAGG CTACCGAGTG ATGTATCGTC AGACCACAGG CCTGCAGGCT
    ACGACAACAT GGCAGAATTT AGATGCCAAA GTCCCAACCG AGCGAAGTGC TGTCTTGGTC
    AACCTGAAAA AGGGAGTGAC TTATGAAATT AAAGTTCGAC CATATTTTAA TGAGTTCCAA
    GGAATGGACA GTGAATCTAA AATAGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCTCCA
    CAATCTGTCA CTGTGCTGAC AGTTGGAAGC CACAATAGCA CAAGTATTAG TGTTTCCTGG
    GATCCTCCTC CTCCAGATCA TCAGAATGGA ATCATCCAAG AATACAAGAT CTGGTGTCTG
    GGGAATGAAA CACGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG ATCTGTGATA
    ATTGGTGGAT TGTTCCCAGG TATTCAGTAC CGGGTAGAGG TTGCAGCTAG TACAAGTGCA
    GGGGTTGGAG TAAAAAGTGA GCCGCAGCCG ATAATAATTG GGGGACGTAA TGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTTGTGAA GCAACCAGCC
    TTTATAGCTG GAATTGGTGG TGCCTGCTGG GTAATTTTGA TGGGTTTTAG CATCTGGTTG
    TATTGGCGAA GAAAGAAGAG AAAGGGACTC AGTAACTATG CTGTTACATT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGGAGCCGT CCTGGTCTTC TAAATGCTGG TGATCCCAGT
    TACCCATGGC TTGCTGATTC ATGGCCAGCC ACGAGCTTAC CAGTAAACAA TAGCAATAGT
    GGCCCAAATG AGATTGGGAA TTTTGGACGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA
    GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGCAGCAT TGACTTCACG
    ACCAAAACCA CTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACAACA
    CAGATCTTGC ATTCCAATAG CATACATGAA TTGGCTGTCG ATCTGCCGGA CCCACAGTGG
    AAGAGCTCAA TTCAGCAAAA AACGGACCTG ATGGGCTTTG GATATTCTCT CCCTGATCAG
    AACAAGGGTA ACAACGCCTT ACTTTACATC CCTGACTACC GATTGGCTGA GGGATTGTCT
    AATAGAATGC CACACAACCA GTCACAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA
    GAGAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAC TAAAAACTCT
    TCTAAACCAC AGAAAAACAA TGGATCGACT TGGGCCAATG TACCCCTACC TCCTCCCCCA
    GTCCAGCCCC TTCCTGGTAC AGAGCTGGAA CACTATGCAG TGGAACCGCA AGAAAATGGG
    TATGACAGTG ATAGCTGGTG CCCGCCATTA CCGGTACAAA CATACTTACA CCAGGGTATG
    GAAGATGAAC TGGAAGAAGA TGATGATCGT GTCCCAACAC CTCCTGTCCG AGGCGTGGCT
    TCTTCTCCTG CTATTTCCTT CGGACAGCAG TCCACTGCAA CTCTTACTCC ATCCCCACGG
    GAAGAGATGC AACCCATGCT GCAAGCTCAC CTGGATGAGT TGACAAGAGC CTATCAGTTT
    GATATAGCGA AGCAAACATG GCACATTCAA AGCAATAATC AACCTCCACA GCCCCCGGTT
    CCACCATTAG GTTACATGTC CGGAGCCTTG ATTTCCGATT TGGAAACAGA TGTTCCAGAT
    GATGACGCTG ATGATGAGGA GGAAGCTTTA GAAATCCCCA GACCCCTGAG AGCACTAGAG
    CAGACACCCG GATCCAGTAT GGACAATCTA GACAGCTCTG TGACAGGCAA AGCTTTTACC
    TCCTCTCAAA GACCTCGACC AACCAGCCCA TTTTCTACTG ACAGTAATAC CAGTGCAGCC
    CTGAGTCAAA ATCAGAGGCC TCGGCCCACT AAAAAACACA AGGGAGGGCG AATGGATCAA
    CAACCAGCAT TGCCTCATCG AAGGGAAGGA ATGACAGATG ATCTTCCACC ACCACCAGAT
    CCCCCTCCAG GTCAGGGTTT AAGGCAGCAA ATAGGCCCGA GCCAGCATGC TGGTAACATG
    GAAAACTCAA CAGAGAGAAA AGGAAGCTCT CTGGAGACAC CACAAGCATC CAACTTAGAA
    GACACAAAGA GCTCATTGGA TTGTCCAGCT AGAACGTCCC TAGAGTGGCA GCGACAAACC
    CAAGAATGGA TAAACTCCAC AGAACGCCAA GAAGATATAC GGAAAGCCCT ACACAAACAA
    AGTGTTGGAC CAGAGGAAAC ATTGGTGCCA TACAGCAAGC CCAGTTTCCC ATCTCCGGGA
    GGTCACAGCT CATCAGGAAC AGCATCTTCT AAAGGATCCA CTGGACCTAG GAAAGCCGAG
    GCGTTGAGGG GAAGTCACCA ACGCAATGCC AGTGACCTTC TTGACATAGG ATATATGGGC
    TCAAATAGTC AAGGACAGTT TACAGGTGAA TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965208.1
    CDS210..4805
    Translation

    Target ORF information:

    RefSeq Version XM_014109733.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109733.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGACCAGAA GACAAGGAAG TGTTACTAGA AGAATGTGGA CATGGACTCC GGAACTGCTA 
    ATGGTGACTG TGATCCTCTG GGGTCAGCAA GGAAATGGAC AAGGCCAAGG ATCTCGTCTT
    CGCCAAGAAG ACTTCCCTCC GCGGATTGTG GAGCACCCTT CAGATGTCAT TGTCTCTAAG
    GGAGAGCCCA CCACCTTGAA CTGTAAGGCA GAGGGCCGGC CAACGCCCAC CATTGAGTGG
    TACAAGGATG GCGAGCGGGT GGAGACTGAC AAGGATGATC CCCGGTCTCA CAGGATGCTT
    CTGCCCAGCG GATCCTTATT TTTCTTGCGC ATTGTTCATG GGCGCAGAAG CAAACCCGAT
    GAAGGAAGCT ACGTCTGTGT TGCGAGGAAC TATCTCGGTG AAGCAGTAAG TCGAAATGCA
    TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGGCAAA ATCCCACAGA TGTTGTAGTG
    GCAGCCGGAG AGCCTGCAAT CCTGGAGTGC CAACCTCCCA GGGGACACCC AGAACCCACG
    ATCTACTGGA AAAAAGACAA AGTCCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT
    GGTGGAAAGC TGATGATATC CAATACCAGG AAAAGTGATG CAGGGATGTA TACCTGTGTT
    GGCACCAATA TGGTGGGAGA AAGAGACAGT GATCCAGCAG AGCTGACTGT CTTTGAACGA
    CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AAGAAGAAGC TGTAGAATTT
    CGTTGTCAGG TCCAAGGAGA TCCTCAACCA ACTGTGCGGT GGAAAAAGGA TGATGCAGAC
    CTGCCAAGAG GAAGGTATGA CATCAAAGAT GATTACACAC TGAGAATTAA AAAGGCCATG
    AGTACAGATG AAGGCACCTA TCTGTGTATT GCCGAGAATC GGGTTGGAAA AGTAGAAGCC
    TCTGCTTCTC TAACAGTCCG AGCTCGCCCT GTTGCTCCTC CACAGTTTGT GGTTAGGCCA
    AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTTACATTTC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAAAAAGAA GGCAGCCAGA ACTTGCTTTT TCCAAATCAA
    CCCCAGCAAC CCAACAGTAG ATGCTCTGTT TCGCCAACTG GAGACCTCAC AATTACCAAC
    ATTCAGCGCT CCGATGCGGG TTACTATATC TGCCAGGCAT TAACTGTGGC AGGAAGCATT
    TTAGCAAAAG CTCAACTGGA AGTTACTGAT GTTTTAACAG ATAGACCTCC ACCTATAATT
    CTACAAGGCC CTGCAAACCA AACTCTAGCA GTTGATGGTA CAGCATTACT GAAATGTAAA
    GCTACTGGGG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCTGGGT
    AGAGATTCAA GAGCAACTAT ACAAGAACAA GGAACATTGC AGATTAAGAA TTTACGGATT
    TCTGATACTG GTACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGGGAGAC TTCGTGGAGT
    GCTGTGCTGG ATGTGACAGA ATCTGGAGCA ACAATCAGTA AAAATTATGA CTTAAGTGAC
    CTACCAGGGC CACCATCCAA ACCACAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG
    TCCTGGCAAC CAGGTACCCC TGGAGCCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC
    AGCCAATCGG TGAGCAATAG CTGGCAGACG GTGGCAAACC ATGTAAAGAC CACGCTCTAT
    ACAGTGAGAG GATTGCGGCC CAATACGATC TATTTATTCA TGGTCAGAGC TATCAACCCC
    CAAGGTCTAA GCGACCCAAG CCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA
    CCAGCACAAG GAGTGGACCA TAGACAAGTA CAGAAAGAAC TAGGAGATGT CATTGTCCGT
    CTTCATACTC CAGTTGTGTT GACTCCCACC ACTGTTCAGG TCACATGGAC GGTTGATCGC
    CAGCCCCAGT TTATTCAAGG CTACCGAGTG ATGTATCGTC AGACCACAGG CCTGCAGGCT
    ACGACAACAT GGCAGAATTT AGATGCCAAA GTCCCAACCG AGCGAAGTGC TGTCTTGGTC
    AACCTGAAAA AGGGAGTGAC TTATGAAATT AAAGTTCGAC CATATTTTAA TGAGTTCCAA
    GGAATGGACA GTGAATCTAA AATAGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCTCCA
    CAATCTGTCA CTGTGCTGAC AGTTGGAAGC CACAATAGCA CAAGTATTAG TGTTTCCTGG
    GATCCTCCTC CTCCAGATCA TCAGAATGGA ATCATCCAAG AATACAAGAT CTGGTGTCTG
    GGGAATGAAA CACGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG ATCTGTGATA
    ATTGGTGGAT TGTTCCCAGG TATTCAGTAC CGGGTAGAGG TTGCAGCTAG TACAAGTGCA
    GGGGTTGGAG TAAAAAGTGA GCCGCAGCCG ATAATAATTG GGGGACGTAA TGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTTGTGAA GCAACCAGCC
    TTTATAGCTG GAATTGGTGG TGCCTGCTGG GTAATTTTGA TGGGTTTTAG CATCTGGTTG
    TATTGGCGAA GAAAGAAGAG AAAGGGACTC AGTAACTATG CTGTTACATT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGGAGCCGT CCTGGTCTTC TAAATGCTGG TGATCCCAGT
    TACCCATGGC TTGCTGATTC ATGGCCAGCC ACGAGCTTAC CAGTAAACAA TAGCAATAGT
    GGCCCAAATG AGATTGGGAA TTTTGGACGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA
    GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGCAGCAT TGACTTCACG
    ACCAAAACCA CTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACAACA
    CAGATCTTGC ATTCCAATAG CATACATGAA TTGGCTGTCG ATCTGCCGGA CCCACAGTGG
    AAGAGCTCAA TTCAGCAAAA AACGGACCTG ATGGGCTTTG GATATTCTCT CCCTGATCAG
    AACAAGGGTA ACAACGCCTT ACTTTACATC CCTGACTACC GATTGGCTGA GGGATTGTCT
    AATAGAATGC CACACAACCA GTCACAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA
    GAGAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAC TAAAAACTCT
    TCTAAACCAC AGAAAAACAA TGGATCGACT TGGGCCAATG TACCCCTACC TCCTCCCCCA
    GTCCAGCCCC TTCCTGGTAC AGAGCTGGAA CACTATGCAG TGGAACCGCA AGAAAATGGG
    TATGACAGTG ATAGCTGGTG CCCGCCATTA CCGGTACAAA CATACTTACA CCAGGGTATG
    GAAGATGAAC TGGAAGAAGA TGATGATCGT GTCCCAACAC CTCCTGTCCG AGGCGTGGCT
    TCTTCTCCTG CTATTTCCTT CGGACAGCAG TCCACTGCAA CTCTTACTCC ATCCCCACGG
    GAAGAGATGC AACCCATGCT GCAAGCTCAC CTGGATGAGT TGACAAGAGC CTATCAGTTT
    GATATAGCGA AGCAAACATG GCACATTCAA AGCAATAATC AACCTCCACA GCCCCCGGTT
    CCACCATTAG GTTACATGTC CGGAGCCTTG ATTTCCGATT TGGAAACAGA TGTTCCAGAT
    GATGACGCTG ATGATGAGGA GGAAGCTTTA GAAATCCCCA GACCCCTGAG AGCACTAGAG
    CAGACACCCG GATCCAGTAT GGACAATCTA GACAGCTCTG TGACAGGCAA AGCTTTTACC
    TCCTCTCAAA GACCTCGACC AACCAGCCCA TTTTCTACTG ACAGTAATAC CAGTGCAGCC
    CTGAGTCAAA ATCAGAGGCC TCGGCCCACT AAAAAACACA AGGGAGGGCG AATGGATCAA
    CAACCAGCAT TGCCTCATCG AAGGGAAGGA ATGACAGATG ATCTTCCACC ACCACCAGAT
    CCCCCTCCAG GTCAGGGTTT AAGGCAGCAA ATAGGCCCGA GCCAGCATGC TGGTAACATG
    GAAAACTCAA CAGAGAGAAA AGGAAGCTCT CTGGAGACAC CACAAGCATC CAACTTAGAA
    GACACAAAGA GCTCATTGGA TTGTCCAGCT AGAACGTCCC TAGAGTGGCA GCGACAAACC
    CAAGAATGGA TAAACTCCAC AGAACGCCAA GAAGATATAC GGAAAGCCCT ACACAAACAA
    AGTGTTGGAC CAGAGGAAAC ATTGGTGCCA TACAGCAAGC CCAGTTTCCC ATCTCCGGGA
    GGTCACAGCT CATCAGGAAC AGCATCTTCT AAAGGATCCA CTGGACCTAG GAAAGCCGAG
    GCGTTGAGGG GAAGTCACCA ACGCAATGCC AGTGACCTTC TTGACATAGG ATATATGGGC
    TCAAATAGTC AAGGACAGTT TACAGGTGAA TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44452
    Clone ID Related Accession (Same CDS sequence) XM_014109734.1
    Accession Version XM_014109734.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4548bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product roundabout homolog 2 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGTCGGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TGTTATATGT TCCGGTTGAT 
    GGATCTCGTC TTCGCCAAGA AGACTTCCCT CCGCGGATTG TGGAGCACCC TTCAGATGTC
    ATTGTCTCTA AGGGAGAGCC CACCACCTTG AACTGTAAGG CAGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAGGA TGGCGAGCGG GTGGAGACTG ACAAGGATGA TCCCCGGTCT
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTTTTCTTGC GCATTGTTCA TGGGCGCAGA
    AGCAAACCCG ATGAAGGAAG CTACGTCTGT GTTGCGAGGA ACTATCTCGG TGAAGCAGTA
    AGTCGAAATG CATCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGGCA AAATCCCACA
    GATGTTGTAG TGGCAGCCGG AGAGCCTGCA ATCCTGGAGT GCCAACCTCC CAGGGGACAC
    CCAGAACCCA CGATCTACTG GAAAAAAGAC AAAGTCCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA GCTGATGATA TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACCTGTG TTGGCACCAA TATGGTGGGA GAAAGAGACA GTGATCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAAGAAGAA
    GCTGTAGAAT TTCGTTGTCA GGTCCAAGGA GATCCTCAAC CAACTGTGCG GTGGAAAAAG
    GATGATGCAG ACCTGCCAAG AGGAAGGTAT GACATCAAAG ATGATTACAC ACTGAGAATT
    AAAAAGGCCA TGAGTACAGA TGAAGGCACC TATCTGTGTA TTGCCGAGAA TCGGGTTGGA
    AAAGTAGAAG CCTCTGCTTC TCTAACAGTC CGAGCTCGCC CTGTTGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTTACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAAAAAG AAGGCAGCCA GAACTTGCTT
    TTTCCAAATC AACCCCAGCA ACCCAACAGT AGATGCTCTG TTTCGCCAAC TGGAGACCTC
    ACAATTACCA ACATTCAGCG CTCCGATGCG GGTTACTATA TCTGCCAGGC ATTAACTGTG
    GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAAGTTACTG ATGTTTTAAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCTGCAAAC CAAACTCTAG CAGTTGATGG TACAGCATTA
    CTGAAATGTA AAGCTACTGG GGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCTGG GTAGAGATTC AAGAGCAACT ATACAAGAAC AAGGAACATT GCAGATTAAG
    AATTTACGGA TTTCTGATAC TGGTACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGGGAG
    ACTTCGTGGA GTGCTGTGCT GGATGTGACA GAATCTGGAG CAACAATCAG TAAAAATTAT
    GACTTAAGTG ACCTACCAGG GCCACCATCC AAACCACAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA ACCAGGTACC CCTGGAGCCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CGGTGGCAAA CCATGTAAAG
    ACCACGCTCT ATACAGTGAG AGGATTGCGG CCCAATACGA TCTATTTATT CATGGTCAGA
    GCTATCAACC CCCAAGGTCT AAGCGACCCA AGCCCCATGT CAGATCCTGT GCGCACACAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CATAGACAAG TACAGAAAGA ACTAGGAGAT
    GTCATTGTCC GTCTTCATAC TCCAGTTGTG TTGACTCCCA CCACTGTTCA GGTCACATGG
    ACGGTTGATC GCCAGCCCCA GTTTATTCAA GGCTACCGAG TGATGTATCG TCAGACCACA
    GGCCTGCAGG CTACGACAAC ATGGCAGAAT TTAGATGCCA AAGTCCCAAC CGAGCGAAGT
    GCTGTCTTGG TCAACCTGAA AAAGGGAGTG ACTTATGAAA TTAAAGTTCG ACCATATTTT
    AATGAGTTCC AAGGAATGGA CAGTGAATCT AAAATAGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCTC CACAATCTGT CACTGTGCTG ACAGTTGGAA GCCACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CATCAGAATG GAATCATCCA AGAATACAAG
    ATCTGGTGTC TGGGGAATGA AACACGATTC CATATCAACA AAACTGTGGA TGCAGCCATT
    CGATCTGTGA TAATTGGTGG ATTGTTCCCA GGTATTCAGT ACCGGGTAGA GGTTGCAGCT
    AGTACAAGTG CAGGGGTTGG AGTAAAAAGT GAGCCGCAGC CGATAATAAT TGGGGGACGT
    AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTTGTG
    AAGCAACCAG CCTTTATAGC TGGAATTGGT GGTGCCTGCT GGGTAATTTT GATGGGTTTT
    AGCATCTGGT TGTATTGGCG AAGAAAGAAG AGAAAGGGAC TCAGTAACTA TGCTGTTACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGGAGCC GTCCTGGTCT TCTAAATGCT
    GGTGATCCCA GTTACCCATG GCTTGCTGAT TCATGGCCAG CCACGAGCTT ACCAGTAAAC
    AATAGCAATA GTGGCCCAAA TGAGATTGGG AATTTTGGAC GTGGAGATGT GCTGCCACCA
    GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CGACCAAAAC CACTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACAA CACAGATCTT GCATTCCAAT AGCATACATG AATTGGCTGT CGATCTGCCG
    GACCCACAGT GGAAGAGCTC AATTCAGCAA AAAACGGACC TGATGGGCTT TGGATATTCT
    CTCCCTGATC AGAACAAGGG TAACAACGCC TTACTTTACA TCCCTGACTA CCGATTGGCT
    GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCACAGG ATTTCAGCAC CACCAGCTCT
    CACAACAGCT CAGAGAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA
    ACTAAAAACT CTTCTAAACC ACAGAAAAAC AATGGATCGA CTTGGGCCAA TGTACCCCTA
    CCTCCTCCCC CAGTCCAGCC CCTTCCTGGT ACAGAGCTGG AACACTATGC AGTGGAACCG
    CAAGAAAATG GGTATGACAG TGATAGCTGG TGCCCGCCAT TACCGGTACA AACATACTTA
    CACCAGGGTA TGGAAGATGA ACTGGAAGAA GATGATGATC GTGTCCCAAC ACCTCCTGTC
    CGAGGCGTGG CTTCTTCTCC TGCTATTTCC TTCGGACAGC AGTCCACTGC AACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACCTGGATGA GTTGACAAGA
    GCCTATCAGT TTGATATAGC GAAGCAAACA TGGCACATTC AAAGCAATAA TCAACCTCCA
    CAGCCCCCGG TTCCACCATT AGGTTACATG TCCGGAGCCT TGATTTCCGA TTTGGAAACA
    GATGTTCCAG ATGATGACGC TGATGATGAG GAGGAAGCTT TAGAAATCCC CAGACCCCTG
    AGAGCACTAG AGCAGACACC CGGATCCAGT ATGGACAATC TAGACAGCTC TGTGACAGGC
    AAAGCTTTTA CCTCCTCTCA AAGACCTCGA CCAACCAGCC CATTTTCTAC TGACAGTAAT
    ACCAGTGCAG CCCTGAGTCA AAATCAGAGG CCTCGGCCCA CTAAAAAACA CAAGGGAGGG
    CGAATGGATC AACAACCAGC ATTGCCTCAT CGAAGGGAAG GAATGACAGA TGATCTTCCA
    CCACCACCAG ATCCCCCTCC AGGTCAGGGT TTAAGGCAGC AAATAGGCCC GAGCCAGCAT
    GCTGGTAACA TGGAAAACTC AACAGAGAGA AAAGGAAGCT CTCTGGAGAC ACCACAAGCA
    TCCAACTTAG AAGACACAAA GAGCTCATTG GATTGTCCAG CTAGAACGTC CCTAGAGTGG
    CAGCGACAAA CCCAAGAATG GATAAACTCC ACAGAACGCC AAGAAGATAT ACGGAAAGCC
    CTACACAAAC AAAGTGTTGG ACCAGAGGAA ACATTGGTGC CATACAGCAA GCCCAGTTTC
    CCATCTCCGG GAGGTCACAG CTCATCAGGA ACAGCATCTT CTAAAGGATC CACTGGACCT
    AGGAAAGCCG AGGCGTTGAG GGGAAGTCAC CAACGCAATG CCAGTGACCT TCTTGACATA
    GGATATATGG GCTCAAATAG TCAAGGACAG TTTACAGGTG AATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965209.1
    CDS814..5361
    Translation

    Target ORF information:

    RefSeq Version XM_014109734.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109734.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGTCGGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TGTTATATGT TCCGGTTGAT 
    GGATCTCGTC TTCGCCAAGA AGACTTCCCT CCGCGGATTG TGGAGCACCC TTCAGATGTC
    ATTGTCTCTA AGGGAGAGCC CACCACCTTG AACTGTAAGG CAGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAGGA TGGCGAGCGG GTGGAGACTG ACAAGGATGA TCCCCGGTCT
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTTTTCTTGC GCATTGTTCA TGGGCGCAGA
    AGCAAACCCG ATGAAGGAAG CTACGTCTGT GTTGCGAGGA ACTATCTCGG TGAAGCAGTA
    AGTCGAAATG CATCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGGCA AAATCCCACA
    GATGTTGTAG TGGCAGCCGG AGAGCCTGCA ATCCTGGAGT GCCAACCTCC CAGGGGACAC
    CCAGAACCCA CGATCTACTG GAAAAAAGAC AAAGTCCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA GCTGATGATA TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACCTGTG TTGGCACCAA TATGGTGGGA GAAAGAGACA GTGATCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAAGAAGAA
    GCTGTAGAAT TTCGTTGTCA GGTCCAAGGA GATCCTCAAC CAACTGTGCG GTGGAAAAAG
    GATGATGCAG ACCTGCCAAG AGGAAGGTAT GACATCAAAG ATGATTACAC ACTGAGAATT
    AAAAAGGCCA TGAGTACAGA TGAAGGCACC TATCTGTGTA TTGCCGAGAA TCGGGTTGGA
    AAAGTAGAAG CCTCTGCTTC TCTAACAGTC CGAGCTCGCC CTGTTGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTTACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAAAAAG AAGGCAGCCA GAACTTGCTT
    TTTCCAAATC AACCCCAGCA ACCCAACAGT AGATGCTCTG TTTCGCCAAC TGGAGACCTC
    ACAATTACCA ACATTCAGCG CTCCGATGCG GGTTACTATA TCTGCCAGGC ATTAACTGTG
    GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAAGTTACTG ATGTTTTAAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCTGCAAAC CAAACTCTAG CAGTTGATGG TACAGCATTA
    CTGAAATGTA AAGCTACTGG GGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCTGG GTAGAGATTC AAGAGCAACT ATACAAGAAC AAGGAACATT GCAGATTAAG
    AATTTACGGA TTTCTGATAC TGGTACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGGGAG
    ACTTCGTGGA GTGCTGTGCT GGATGTGACA GAATCTGGAG CAACAATCAG TAAAAATTAT
    GACTTAAGTG ACCTACCAGG GCCACCATCC AAACCACAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA ACCAGGTACC CCTGGAGCCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CGGTGGCAAA CCATGTAAAG
    ACCACGCTCT ATACAGTGAG AGGATTGCGG CCCAATACGA TCTATTTATT CATGGTCAGA
    GCTATCAACC CCCAAGGTCT AAGCGACCCA AGCCCCATGT CAGATCCTGT GCGCACACAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CATAGACAAG TACAGAAAGA ACTAGGAGAT
    GTCATTGTCC GTCTTCATAC TCCAGTTGTG TTGACTCCCA CCACTGTTCA GGTCACATGG
    ACGGTTGATC GCCAGCCCCA GTTTATTCAA GGCTACCGAG TGATGTATCG TCAGACCACA
    GGCCTGCAGG CTACGACAAC ATGGCAGAAT TTAGATGCCA AAGTCCCAAC CGAGCGAAGT
    GCTGTCTTGG TCAACCTGAA AAAGGGAGTG ACTTATGAAA TTAAAGTTCG ACCATATTTT
    AATGAGTTCC AAGGAATGGA CAGTGAATCT AAAATAGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCTC CACAATCTGT CACTGTGCTG ACAGTTGGAA GCCACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CATCAGAATG GAATCATCCA AGAATACAAG
    ATCTGGTGTC TGGGGAATGA AACACGATTC CATATCAACA AAACTGTGGA TGCAGCCATT
    CGATCTGTGA TAATTGGTGG ATTGTTCCCA GGTATTCAGT ACCGGGTAGA GGTTGCAGCT
    AGTACAAGTG CAGGGGTTGG AGTAAAAAGT GAGCCGCAGC CGATAATAAT TGGGGGACGT
    AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTTGTG
    AAGCAACCAG CCTTTATAGC TGGAATTGGT GGTGCCTGCT GGGTAATTTT GATGGGTTTT
    AGCATCTGGT TGTATTGGCG AAGAAAGAAG AGAAAGGGAC TCAGTAACTA TGCTGTTACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGGAGCC GTCCTGGTCT TCTAAATGCT
    GGTGATCCCA GTTACCCATG GCTTGCTGAT TCATGGCCAG CCACGAGCTT ACCAGTAAAC
    AATAGCAATA GTGGCCCAAA TGAGATTGGG AATTTTGGAC GTGGAGATGT GCTGCCACCA
    GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CGACCAAAAC CACTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACAA CACAGATCTT GCATTCCAAT AGCATACATG AATTGGCTGT CGATCTGCCG
    GACCCACAGT GGAAGAGCTC AATTCAGCAA AAAACGGACC TGATGGGCTT TGGATATTCT
    CTCCCTGATC AGAACAAGGG TAACAACGCC TTACTTTACA TCCCTGACTA CCGATTGGCT
    GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCACAGG ATTTCAGCAC CACCAGCTCT
    CACAACAGCT CAGAGAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA
    ACTAAAAACT CTTCTAAACC ACAGAAAAAC AATGGATCGA CTTGGGCCAA TGTACCCCTA
    CCTCCTCCCC CAGTCCAGCC CCTTCCTGGT ACAGAGCTGG AACACTATGC AGTGGAACCG
    CAAGAAAATG GGTATGACAG TGATAGCTGG TGCCCGCCAT TACCGGTACA AACATACTTA
    CACCAGGGTA TGGAAGATGA ACTGGAAGAA GATGATGATC GTGTCCCAAC ACCTCCTGTC
    CGAGGCGTGG CTTCTTCTCC TGCTATTTCC TTCGGACAGC AGTCCACTGC AACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACCTGGATGA GTTGACAAGA
    GCCTATCAGT TTGATATAGC GAAGCAAACA TGGCACATTC AAAGCAATAA TCAACCTCCA
    CAGCCCCCGG TTCCACCATT AGGTTACATG TCCGGAGCCT TGATTTCCGA TTTGGAAACA
    GATGTTCCAG ATGATGACGC TGATGATGAG GAGGAAGCTT TAGAAATCCC CAGACCCCTG
    AGAGCACTAG AGCAGACACC CGGATCCAGT ATGGACAATC TAGACAGCTC TGTGACAGGC
    AAAGCTTTTA CCTCCTCTCA AAGACCTCGA CCAACCAGCC CATTTTCTAC TGACAGTAAT
    ACCAGTGCAG CCCTGAGTCA AAATCAGAGG CCTCGGCCCA CTAAAAAACA CAAGGGAGGG
    CGAATGGATC AACAACCAGC ATTGCCTCAT CGAAGGGAAG GAATGACAGA TGATCTTCCA
    CCACCACCAG ATCCCCCTCC AGGTCAGGGT TTAAGGCAGC AAATAGGCCC GAGCCAGCAT
    GCTGGTAACA TGGAAAACTC AACAGAGAGA AAAGGAAGCT CTCTGGAGAC ACCACAAGCA
    TCCAACTTAG AAGACACAAA GAGCTCATTG GATTGTCCAG CTAGAACGTC CCTAGAGTGG
    CAGCGACAAA CCCAAGAATG GATAAACTCC ACAGAACGCC AAGAAGATAT ACGGAAAGCC
    CTACACAAAC AAAGTGTTGG ACCAGAGGAA ACATTGGTGC CATACAGCAA GCCCAGTTTC
    CCATCTCCGG GAGGTCACAG CTCATCAGGA ACAGCATCTT CTAAAGGATC CACTGGACCT
    AGGAAAGCCG AGGCGTTGAG GGGAAGTCAC CAACGCAATG CCAGTGACCT TCTTGACATA
    GGATATATGG GCTCAAATAG TCAAGGACAG TTTACAGGTG AATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29287
    Clone ID Related Accession (Same CDS sequence) XM_005638758.2
    Accession Version XM_005638758.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4611bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product roundabout homolog 2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638758.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTTACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAAAAAGA AGGCAGCCAG
    AACTTGCTTT TTCCAAATCA ACCCCAGCAA CCCAACAGTA GATGCTCTGT TTCGCCAACT
    GGAGACCTCA CAATTACCAA CATTCAGCGC TCCGATGCGG GTTACTATAT CTGCCAGGCA
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AAGTTACTGA TGTTTTAACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCTGCAAACC AAACTCTAGC AGTTGATGGT
    ACAGCATTAC TGAAATGTAA AGCTACTGGG GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCTGGG TAGAGATTCA AGAGCAACTA TACAAGAACA AGGAACATTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGTACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGGGAGA CTTCGTGGAG TGCTGTGCTG GATGTGACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ACTTAAGTGA CCTACCAGGG CCACCATCCA AACCACAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAA CCAGGTACCC CTGGAGCCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC GGTGGCAAAC
    CATGTAAAGA CCACGCTCTA TACAGTGAGA GGATTGCGGC CCAATACGAT CTATTTATTC
    ATGGTCAGAG CTATCAACCC CCAAGGTCTA AGCGACCCAA GCCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ATAGACAAGT ACAGAAAGAA
    CTAGGAGATG TCATTGTCCG TCTTCATACT CCAGTTGTGT TGACTCCCAC CACTGTTCAG
    GTCACATGGA CGGTTGATCG CCAGCCCCAG TTTATTCAAG GCTACCGAGT GATGTATCGT
    CAGACCACAG GCCTGCAGGC TACGACAACA TGGCAGAATT TAGATGCCAA AGTCCCAACC
    GAGCGAAGTG CTGTCTTGGT CAACCTGAAA AAGGGAGTGA CTTATGAAAT TAAAGTTCGA
    CCATATTTTA ATGAGTTCCA AGGAATGGAC AGTGAATCTA AAATAGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCTCC ACAATCTGTC ACTGTGCTGA CAGTTGGAAG CCACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ATCAGAATGG AATCATCCAA
    GAATACAAGA TCTGGTGTCT GGGGAATGAA ACACGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GATCTGTGAT AATTGGTGGA TTGTTCCCAG GTATTCAGTA CCGGGTAGAG
    GTTGCAGCTA GTACAAGTGC AGGGGTTGGA GTAAAAAGTG AGCCGCAGCC GATAATAATT
    GGGGGACGTA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTTGTGA AGCAACCAGC CTTTATAGCT GGAATTGGTG GTGCCTGCTG GGTAATTTTG
    ATGGGTTTTA GCATCTGGTT GTATTGGCGA AGAAAGAAGA GAAAGGGACT CAGTAACTAT
    GCTGTTACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCTGGTCTT
    CTAAATGCTG GTGATCCCAG TTACCCATGG CTTGCTGATT CATGGCCAGC CACGAGCTTA
    CCAGTAAACA ATAGCAATAG TGGCCCAAAT GAGATTGGGA ATTTTGGACG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC GACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACAAC ACAGATCTTG CATTCCAATA GCATACATGA ATTGGCTGTC
    GATCTGCCGG ACCCACAGTG GAAGAGCTCA ATTCAGCAAA AAACGGACCT GATGGGCTTT
    GGATATTCTC TCCCTGATCA GAACAAGGGT AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAGAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCGAC TTGGGCCAAT
    GTACCCCTAC CTCCTCCCCC AGTCCAGCCC CTTCCTGGTA CAGAGCTGGA ACACTATGCA
    GTGGAACCGC AAGAAAATGG GTATGACAGT GATAGCTGGT GCCCGCCATT ACCGGTACAA
    ACATACTTAC ACCAGGGTAT GGAAGATGAA CTGGAAGAAG ATGATGATCG TGTCCCAACA
    CCTCCTGTCC GAGGCGTGGC TTCTTCTCCT GCTATTTCCT TCGGACAGCA GTCCACTGCA
    ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAAGCTCA CCTGGATGAG
    TTGACAAGAG CCTATCAGTT TGATATAGCG AAGCAAACAT GGCACATTCA AAGCAATAAT
    CAACCTCCAC AGCCCCCGGT TCCACCATTA GGTTACATGT CCGGAGCCTT GATTTCCGAT
    TTGGAAACAG ATGTTCCAGA TGATGACGCT GATGATGAGG AGGAAGCTTT AGAAATCCCC
    AGACCCCTGA GAGCACTAGA GCAGACACCC GGATCCAGTA TGGACAATCT AGACAGCTCT
    GTGACAGGCA AAGCTTTTAC CTCCTCTCAA AGACCTCGAC CAACCAGCCC ATTTTCTACT
    GACAGTAATA CCAGTGCAGC CCTGAGTCAA AATCAGAGGC CTCGGCCCAC TAAAAAACAC
    AAGGGAGGGC GAATGGATCA ACAACCAGCA TTGCCTCATC GAAGGGAAGG AATGACAGAT
    GATCTTCCAC CACCACCAGA TCCCCCTCCA GGTCAGGGTT TAAGGCAGCA AATAGGCCCG
    AGCCAGCATG CTGGTAACAT GGAAAACTCA ACAGAGAGAA AAGGAAGCTC TCTGGAGACA
    CCACAAGCAT CCAACTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAGAACGTCC
    CTAGAGTGGC AGCGACAAAC CCAAGAATGG ATAAACTCCA CAGAACGCCA AGAAGATATA
    CGGAAAGCCC TACACAAACA AAGTGTTGGA CCAGAGGAAA CATTGGTGCC ATACAGCAAG
    CCCAGTTTCC CATCTCCGGG AGGTCACAGC TCATCAGGAA CAGCATCTTC TAAAGGATCC
    ACTGGACCTA GGAAAGCCGA GGCGTTGAGG GGAAGTCACC AACGCAATGC CAGTGACCTT
    CTTGACATAG GATATATGGG CTCAAATAGT CAAGGACAGT TTACAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638815.1
    CDS585..5195
    Translation

    Target ORF information:

    RefSeq Version XM_005638758.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris roundabout guidance receptor 2 (ROBO2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638758.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTATCAT TAGAGAAAGT GAGGAGAATT ACTAGACTTG AACTTTTTCT CGTTTTGATG 
    CTCTTTTTCT CTGGACCAAC TCTGAGCAAG CTGACTCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAAGGGG GATCTCGTCT TCGCCAAGAA GACTTCCCTC CGCGGATTGT GGAGCACCCT
    TCAGATGTCA TTGTCTCTAA GGGAGAGCCC ACCACCTTGA ACTGTAAGGC AGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAGGAT GGCGAGCGGG TGGAGACTGA CAAGGATGAT
    CCCCGGTCTC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TTTTCTTGCG CATTGTTCAT
    GGGCGCAGAA GCAAACCCGA TGAAGGAAGC TACGTCTGTG TTGCGAGGAA CTATCTCGGT
    GAAGCAGTAA GTCGAAATGC ATCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGGCAA
    AATCCCACAG ATGTTGTAGT GGCAGCCGGA GAGCCTGCAA TCCTGGAGTG CCAACCTCCC
    AGGGGACACC CAGAACCCAC GATCTACTGG AAAAAAGACA AAGTCCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAG CTGATGATAT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACCTGTGT TGGCACCAAT ATGGTGGGAG AAAGAGACAG TGATCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAAGAAGAAG CTGTAGAATT TCGTTGTCAG GTCCAAGGAG ATCCTCAACC AACTGTGCGG
    TGGAAAAAGG ATGATGCAGA CCTGCCAAGA GGAAGGTATG ACATCAAAGA TGATTACACA
    CTGAGAATTA AAAAGGCCAT GAGTACAGAT GAAGGCACCT ATCTGTGTAT TGCCGAGAAT
    CGGGTTGGAA AAGTAGAAGC CTCTGCTTCT CTAACAGTCC GAGCTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTTACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAAAAAGA AGGCAGCCAG
    AACTTGCTTT TTCCAAATCA ACCCCAGCAA CCCAACAGTA GATGCTCTGT TTCGCCAACT
    GGAGACCTCA CAATTACCAA CATTCAGCGC TCCGATGCGG GTTACTATAT CTGCCAGGCA
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AAGTTACTGA TGTTTTAACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCTGCAAACC AAACTCTAGC AGTTGATGGT
    ACAGCATTAC TGAAATGTAA AGCTACTGGG GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCTGGG TAGAGATTCA AGAGCAACTA TACAAGAACA AGGAACATTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGTACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGGGAGA CTTCGTGGAG TGCTGTGCTG GATGTGACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ACTTAAGTGA CCTACCAGGG CCACCATCCA AACCACAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAA CCAGGTACCC CTGGAGCCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC GGTGGCAAAC
    CATGTAAAGA CCACGCTCTA TACAGTGAGA GGATTGCGGC CCAATACGAT CTATTTATTC
    ATGGTCAGAG CTATCAACCC CCAAGGTCTA AGCGACCCAA GCCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ATAGACAAGT ACAGAAAGAA
    CTAGGAGATG TCATTGTCCG TCTTCATACT CCAGTTGTGT TGACTCCCAC CACTGTTCAG
    GTCACATGGA CGGTTGATCG CCAGCCCCAG TTTATTCAAG GCTACCGAGT GATGTATCGT
    CAGACCACAG GCCTGCAGGC TACGACAACA TGGCAGAATT TAGATGCCAA AGTCCCAACC
    GAGCGAAGTG CTGTCTTGGT CAACCTGAAA AAGGGAGTGA CTTATGAAAT TAAAGTTCGA
    CCATATTTTA ATGAGTTCCA AGGAATGGAC AGTGAATCTA AAATAGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCTCC ACAATCTGTC ACTGTGCTGA CAGTTGGAAG CCACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ATCAGAATGG AATCATCCAA
    GAATACAAGA TCTGGTGTCT GGGGAATGAA ACACGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GATCTGTGAT AATTGGTGGA TTGTTCCCAG GTATTCAGTA CCGGGTAGAG
    GTTGCAGCTA GTACAAGTGC AGGGGTTGGA GTAAAAAGTG AGCCGCAGCC GATAATAATT
    GGGGGACGTA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTTGTGA AGCAACCAGC CTTTATAGCT GGAATTGGTG GTGCCTGCTG GGTAATTTTG
    ATGGGTTTTA GCATCTGGTT GTATTGGCGA AGAAAGAAGA GAAAGGGACT CAGTAACTAT
    GCTGTTACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCTGGTCTT
    CTAAATGCTG GTGATCCCAG TTACCCATGG CTTGCTGATT CATGGCCAGC CACGAGCTTA
    CCAGTAAACA ATAGCAATAG TGGCCCAAAT GAGATTGGGA ATTTTGGACG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC GACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACAAC ACAGATCTTG CATTCCAATA GCATACATGA ATTGGCTGTC
    GATCTGCCGG ACCCACAGTG GAAGAGCTCA ATTCAGCAAA AAACGGACCT GATGGGCTTT
    GGATATTCTC TCCCTGATCA GAACAAGGGT AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAGAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCGAC TTGGGCCAAT
    GTACCCCTAC CTCCTCCCCC AGTCCAGCCC CTTCCTGGTA CAGAGCTGGA ACACTATGCA
    GTGGAACCGC AAGAAAATGG GTATGACAGT GATAGCTGGT GCCCGCCATT ACCGGTACAA
    ACATACTTAC ACCAGGGTAT GGAAGATGAA CTGGAAGAAG ATGATGATCG TGTCCCAACA
    CCTCCTGTCC GAGGCGTGGC TTCTTCTCCT GCTATTTCCT TCGGACAGCA GTCCACTGCA
    ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAAGCTCA CCTGGATGAG
    TTGACAAGAG CCTATCAGTT TGATATAGCG AAGCAAACAT GGCACATTCA AAGCAATAAT
    CAACCTCCAC AGCCCCCGGT TCCACCATTA GGTTACATGT CCGGAGCCTT GATTTCCGAT
    TTGGAAACAG ATGTTCCAGA TGATGACGCT GATGATGAGG AGGAAGCTTT AGAAATCCCC
    AGACCCCTGA GAGCACTAGA GCAGACACCC GGATCCAGTA TGGACAATCT AGACAGCTCT
    GTGACAGGCA AAGCTTTTAC CTCCTCTCAA AGACCTCGAC CAACCAGCCC ATTTTCTACT
    GACAGTAATA CCAGTGCAGC CCTGAGTCAA AATCAGAGGC CTCGGCCCAC TAAAAAACAC
    AAGGGAGGGC GAATGGATCA ACAACCAGCA TTGCCTCATC GAAGGGAAGG AATGACAGAT
    GATCTTCCAC CACCACCAGA TCCCCCTCCA GGTCAGGGTT TAAGGCAGCA AATAGGCCCG
    AGCCAGCATG CTGGTAACAT GGAAAACTCA ACAGAGAGAA AAGGAAGCTC TCTGGAGACA
    CCACAAGCAT CCAACTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAGAACGTCC
    CTAGAGTGGC AGCGACAAAC CCAAGAATGG ATAAACTCCA CAGAACGCCA AGAAGATATA
    CGGAAAGCCC TACACAAACA AAGTGTTGGA CCAGAGGAAA CATTGGTGCC ATACAGCAAG
    CCCAGTTTCC CATCTCCGGG AGGTCACAGC TCATCAGGAA CAGCATCTTC TAAAGGATCC
    ACTGGACCTA GGAAAGCCGA GGCGTTGAGG GGAAGTCACC AACGCAATGC CAGTGACCTT
    CTTGACATAG GATATATGGG CTCAAATAGT CAAGGACAGT TTACAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.