ROBO1 cDNA ORF clone, Canis lupus familiaris(dog)

The following ROBO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ROBO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe283884 XM_022413109.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe283885 XM_022413110.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe283886 XM_022413111.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe283887 XM_022413112.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe283888 XM_022413113.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe283889 XM_022413114.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe283890 XM_022413115.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe44463 XM_014109750.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe44464 XM_014109751.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe44465 XM_014109752.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe44466 XM_014109753.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe44467 XM_014109754.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe44468 XM_014109755.2
Latest version!
Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe44469 XM_014109756.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe29277 XM_544814.5
Latest version!
Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe29280 XM_005638751.2
Latest version!
Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe29281 XM_005638752.2
Latest version!
Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OCe44468 XM_014109755.1 Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe283884
    Clone ID Related Accession (Same CDS sequence) XM_022413109.1
    Accession Version XM_022413109.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5061bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product roundabout homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCTGAGAC CTCCGTGCAG CTCAGTATGC TGCAGGAGGG GCAAGGAGGA AGTGTGCTGT 
    GTGTTGTCCC TGTGTGTTTT TCTGTCACAT GTGGATTTAC TTGTGTCTGG ACATGCTGTG
    ATCGAAGTGG CAACTACCCT TACTACTGTG ACCCCATCAG CAGCTCTGGT GTTGTTTCCG
    ACAGACCCTG AAGACCTAGA GAGGGGGAAC GACAACGGGA CGCCAATCCC CACCTCTGAT
    AATGATGACA ATTCGCTGGG CTACACAGGC TCCCGTCTCC GTCAGGAAGA TTTTCCACCT
    CGTATTGTTG AACACCCTTC AGACCTAATT GTGTCAAAAG GAGAACCTGC AACTCTGAAC
    TGTAAAGCTG AAGGCCGCCC CACGCCCACC ATTGAATGGT ACAAAGGTGG GGAGAGAGTG
    GAGACAGACA AAGATGACCC TCGCTCACAC CGAATGTTGC TGCCAAGTGG ATCTTTATTT
    TTCTTACGTA TAGTACATGG ACGAAAAAGT AGGCCTGATG AAGGGGTCTA CGTCTGTGTG
    GCACGGAATT ACCTCGGAGA GGCTGTGAGC CATAATGCAT CGCTGGAGGT GGCTATACTA
    CGGGATGACT TCAGACAAAA TCCTTCTGAT GTCATGGTGG CAGTGGGGGA ACCTGCTGTG
    ATGGAATGCC AACCCCCACG AGGCCATCCT GAACCCACCA TTTCGTGGAA GAAAGATGGC
    TCTCCACTGG ATGATAAAGA TGAAAGAATA ACTATACGAG GTGGAAAGCT CATGATTACT
    TACACCCGCA AAAGTGATGC TGGCAAATAT GTTTGTGTTG GTACCAACAT GGTTGGGGAA
    CGTGAGAGTG AAGTAGCAGA GCTAACTGTC TTGGAGAGAC CATCATTTGT GAAGAGACCT
    AGTAACTTGG CTGTAACTGT GGATGACAGT GCAGAATTTA AATGTGAGGC TCGAGGTGAC
    CCTGTGCCTA CGGTACGATG GAGGAAAGAC GATGGAGAAC TGCCCAAATC CAGATACGAA
    ATCAGAGATG ATCATACTTT GAAGATTCGG AAAGTGATGG CTGGTGACAT GGGGTCATAT
    ACTTGTGTTG CAGAAAATAT GGTTGGAAAA GCTGAAGCAT CAGCTACTCT GACTGTCCAA
    GTTGGGTCTG AACCTCCACA TTTTGTTGTG AAACCTCGTG ACCAGGTTGT TGCCTTGGGG
    CGAACTGTAA CATTTCAGTG TGAAGCAACC GGAAATCCTC AACCAGCTAT TTTTTGGAGA
    AGAGAGGGGA GTCAGAATCT GCTTTTCTCA TATCAGCCGC CACAGTCAAC TAGCCGCTTT
    TCCGTCTCCC AGACTGGTGA TCTCACTATT ACTAATGTCC AGCGGTCTGA TGTTGGTTAT
    TATATCTGCC AGACTTTAAA TGTTGCCGGA AGTATCATCA CAAAAGCATA TTTGGAAGTT
    ACTGATGTGA TTGCAGACCG GCCTCCCCCA GTCATTCGAC AAGGTCCTGT GAATCAGACT
    GTAGCAGTGG ATGGTACTTT AGTGCTCAGC TGTGTGGCCA CAGGCAGTCC AATGCCCACG
    ATTCTCTGGA GAAAGGATGG AGTCCTTGTT TCAACCCAAG ATTCTAGAAT CAAACAGCTG
    GAGACGGGAG TGCTGCAGAT CCGATACGCT AAGCTGGGTG ACACAGGTCG GTACACCTGC
    ATTGCATCAA CCCCCAGTGG TGAAGCAACA TGGAGTGCTT ACATTGAAGT CCAAGAATTT
    GGAGTTCCAG TTCAGCCTCC ACGACCCACC GACCCAAATT TAATCCCTAG TGCCCCCTCA
    AAACCTGAAG TTACAGATGT CAGCAGAAAT ACAGTAACGT TGTCATGGCA ACCAAATTTG
    AATTCAGGTG CAACTCCAAC ATCTTATATT ATAGAAGCCT TCAGCCATGC ATCTGGTAGC
    AGCTGGCAGA CCGTAGCAGA GAATGTGAAA ACAGAAACAT TTGCCATTAA AGGACTCAAA
    CCTAATGCAA TTTACCTTTT CCTTGTGAGG GCAGCAAATG CCTATGGAAT TAGTGATCCA
    AGCCAGATAT CAGATCCAGT GAAAACACAA GATGTTCCCC CAACAAGTCA GGGAGTAGAT
    CACAAGCAGG TCCAGAGAGA ATTGGGAAAC GTCGTACTGC ACCTTCACAA CCCCACCATC
    CTCTCATCCT CTTCCATCGA AGTGCACTGG ACAGTGGACC AACAATCTCA GTATATTCAA
    GGATATAAAA TTCTTTATCG GCCATCTGGA GGCAACCATG GTGAATCGGA GTGGTTAGTT
    TTTGAAGTGA GGACTCCAAC CAAAAATAGC GTGGTTATCC CTGATCTCAA AAAGGGAGTC
    AACTATGAAA TTAAGGCTCG TCCTTTTTTT AATGAATTTC AAGGAGCAGA TAGTGAAATT
    AAATTTGCCA AAACCTTAGA AGAAGCACCC AGTGCCCCAC CCCAAAGTGT AACCGTATCA
    AAGAATGATG GAAATGGAAC TGCAATTCTT GTTAGTTGGC AGCCACCTCC AGAAGACACT
    CAAAATGGAA TGGTCCAAGA GTATAAGGTT TGGTGTCTGG GCAATGAAAC TCGTTATCAC
    ATAAACAAGA CGGTGGATGG TTCCACCTTT TCTGTGGTCA TTCCTTCTCT GGTTCCTGGG
    ATCCGATACA GCGTAGAAGT AGCAGCCAGC ACGGGAGCTG GAGCTGGGGT GAAGAGCGAG
    CCACAGTTCA TCCAGTTAGA TTCTCATGGA AACCCCGTGT CACCTGAAGA CCAAGTCAGC
    CTTGCTCAGC AGATCTCAGA TGTTGTGAAG CAGCCAGCGT TCATTGCGGG AATCGGGGCC
    GCCTGCTGGA TCATCCTCAT GGTCTTTAGC ATCTGGCTCT ATCGACACCG GAAGAAGAGA
    AATGGACTGA CTAGTACTTA TGCGGGTATC AGAAAAGTCC CGTCTTTTAC CTTCACACCA
    ACAGTAACTT ATCAGAGAGG AGGTGAAGCT GTGAGCAGTG GAGGGAGGCC AGGACTTCTG
    AACATCAGCG AACCTGCCAC ACAGCCCTGG CTGGCAGACA CGTGGCCCAA TACTGGCAAC
    AATCACAATG ACTGCTCTAT CAATTGCTGT ACGGCAGGCA ATGGTAACAG TGATAACAAC
    CTGACCACCT ACAGTCGCCC AGCCGATTGT ATAGCAAATT ATAACAACCA ACTGGATAAC
    AAACAAACAA ATCTGATGCT CCCTGAGTCA ACTGTTTATG GTGATGTGGA CCTTAGTAAC
    AAAATCAATG AGATGAAAAC CTTCAATAGC CCAAATCTGA AGGATGGGCG TTTTGTCAAT
    CCATCAGGGC AGCCCACTCC TTATGCCACC ACCCAGCTCA TCCAGTCAAA CCTCAGCAAC
    AACATGAACA ATGGCAGCGG GGACTCCAGC GAGAAGCACT GGAAACCTCC CGGCCAGCAG
    AAACAAGAAG TGCCACCAGT TCAGTATAAC ATCATGGAGC AAAACAAACT GAACAAAGAT
    TATCGAGCAA ATGACACAGT TCCTCCAACT ATCCCATACA ACCAATCATA TGACCAGAAT
    ACAGGAGGAT CCTACAACAG TTCAGACCGG GGCAGTAGTA CATCTGGGAG TCAAGGGCAC
    AAGAAAGGGG CACGGACACC CAAAGTACCA AAACAAGGTG GCATGAACTG GGCAGACCTG
    CTTCCTCCTC CCCCAGCGCA TCCTCCTCCT CATAGCAATA GTGAAGAGTA CAATATTTCT
    GTAGATGAAA GCTATGACCA AGAAATGCCA TGTCCCGTGC CACCAGCAAG GATGTATTTG
    CAACAGAATG AACTAGAAGA GGAGGAAGAT GAGCGAGGCC CCACTCCCCC TGTGCGGGGA
    GCAGCTTCTT CTCCAGCTGC TGTGTCTTAT AGCCATCAGT CCACTGCTAC TCTGACCCCC
    TCCCCACAAG AAGAACTCCA GCCCATGTTG CAGGACTGCC CGGAGGAGAT TGGCCACATG
    CAGCACCAAC CCGATAGGAG ACGGCAGCCT GTGAGTCCTC CTCCACCACC AAGACCGATT
    TCCCCACCAC ATACCTATGG CTACATTTCA GGACCTCTTG TCTCAGATAT GGATACAGAT
    GCACCAGAAG AGGAAGAAGA TGAAGCAGAC ATGGAAGTGG CCAAGATGCA GACCAGACGG
    CTTTTGTTAC GTGGCCTTGA GCAGACCCCT GCCTCCAGTG TGGGGGACCT TGAAAGCTCT
    GTCACTGGGT CCATGATCAA TGGCTGGGGC TCAGCCTCAG AGGAGGACAA CATTTCCAGT
    GGACGCTCCA GTGTGAGTTC TTCTGATGGC TCCTTCTTCA CTGATGCTGA TTTTGCCCAG
    GCAGTGGCAG CAGCTGCAGA ATATGCTGGT CTTAAAGTGG CACGTCGTCA GATGCAGGAT
    GCTGCTGGTC GCCGACATTT TCATGCATCC CAGTGCCCTC GACCCACAAG TCCTGTCTCT
    ACAGACAGCA ACATGAGTGC TGCTGTAATG CAGAAGGCCA GACCAGCCAA GAAGCAAAAA
    CACCAGCCAG GACATCTGCG CAGAGAAGTC TACACAGATG ATCTACCACC TCCTCCGGTG
    CCACCACCTG CTATAAAATC ACCCACTGTC CAATCCAAGG CACAGCTGGA GGTGCGACCT
    GTTATGGTGC CAAAACTCCC TTCTATAGAA GCAAGAACAG ACCGATCATC AGATAGAAAA
    GTAGGCAGTT ACAAGGGGAG AGAAGTACTG GATGGAAGAC AGGTTGCTGA TATGAGAACC
    AATCCAGGTG ATACCAGAGA AGCACAGGAA CAGCAAAATG ATGGAAAAGG ACGAGTAAAC
    AAGGCAGCAA AACGAGACCT TCCACCAGCA AAGACTCACC TCATCCAAGA GGATATCCTA
    CCTTACTGTA GACCTACTTT TCCGACATCA AATAATCCCA GAGATCCTAG TTCTTCAAGC
    TCAATGTCAT CAAGAGGATC AGGAAGCAGA CAAAGAGAAC AAGCAAATGT AGGTCGAAGA
    AATATCGCAG AAATGCAAGT TCTTGGAGGA TATGAAAGAG GAGAAGATAA TAATGAAGAA
    TTAGAGGAAA CTGAAAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268817.1
    CDS16..5076
    Translation

    Target ORF information:

    RefSeq Version XM_022413109.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413109.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCTGAGAC CTCCGTGCAG CTCAGTATGC TGCAGGAGGG GCAAGGAGGA AGTGTGCTGT 
    GTGTTGTCCC TGTGTGTTTT TCTGTCACAT GTGGATTTAC TTGTGTCTGG ACATGCTGTG
    ATCGAAGTGG CAACTACCCT TACTACTGTG ACCCCATCAG CAGCTCTGGT GTTGTTTCCG
    ACAGACCCTG AAGACCTAGA GAGGGGGAAC GACAACGGGA CGCCAATCCC CACCTCTGAT
    AATGATGACA ATTCGCTGGG CTACACAGGC TCCCGTCTCC GTCAGGAAGA TTTTCCACCT
    CGTATTGTTG AACACCCTTC AGACCTAATT GTGTCAAAAG GAGAACCTGC AACTCTGAAC
    TGTAAAGCTG AAGGCCGCCC CACGCCCACC ATTGAATGGT ACAAAGGTGG GGAGAGAGTG
    GAGACAGACA AAGATGACCC TCGCTCACAC CGAATGTTGC TGCCAAGTGG ATCTTTATTT
    TTCTTACGTA TAGTACATGG ACGAAAAAGT AGGCCTGATG AAGGGGTCTA CGTCTGTGTG
    GCACGGAATT ACCTCGGAGA GGCTGTGAGC CATAATGCAT CGCTGGAGGT GGCTATACTA
    CGGGATGACT TCAGACAAAA TCCTTCTGAT GTCATGGTGG CAGTGGGGGA ACCTGCTGTG
    ATGGAATGCC AACCCCCACG AGGCCATCCT GAACCCACCA TTTCGTGGAA GAAAGATGGC
    TCTCCACTGG ATGATAAAGA TGAAAGAATA ACTATACGAG GTGGAAAGCT CATGATTACT
    TACACCCGCA AAAGTGATGC TGGCAAATAT GTTTGTGTTG GTACCAACAT GGTTGGGGAA
    CGTGAGAGTG AAGTAGCAGA GCTAACTGTC TTGGAGAGAC CATCATTTGT GAAGAGACCT
    AGTAACTTGG CTGTAACTGT GGATGACAGT GCAGAATTTA AATGTGAGGC TCGAGGTGAC
    CCTGTGCCTA CGGTACGATG GAGGAAAGAC GATGGAGAAC TGCCCAAATC CAGATACGAA
    ATCAGAGATG ATCATACTTT GAAGATTCGG AAAGTGATGG CTGGTGACAT GGGGTCATAT
    ACTTGTGTTG CAGAAAATAT GGTTGGAAAA GCTGAAGCAT CAGCTACTCT GACTGTCCAA
    GTTGGGTCTG AACCTCCACA TTTTGTTGTG AAACCTCGTG ACCAGGTTGT TGCCTTGGGG
    CGAACTGTAA CATTTCAGTG TGAAGCAACC GGAAATCCTC AACCAGCTAT TTTTTGGAGA
    AGAGAGGGGA GTCAGAATCT GCTTTTCTCA TATCAGCCGC CACAGTCAAC TAGCCGCTTT
    TCCGTCTCCC AGACTGGTGA TCTCACTATT ACTAATGTCC AGCGGTCTGA TGTTGGTTAT
    TATATCTGCC AGACTTTAAA TGTTGCCGGA AGTATCATCA CAAAAGCATA TTTGGAAGTT
    ACTGATGTGA TTGCAGACCG GCCTCCCCCA GTCATTCGAC AAGGTCCTGT GAATCAGACT
    GTAGCAGTGG ATGGTACTTT AGTGCTCAGC TGTGTGGCCA CAGGCAGTCC AATGCCCACG
    ATTCTCTGGA GAAAGGATGG AGTCCTTGTT TCAACCCAAG ATTCTAGAAT CAAACAGCTG
    GAGACGGGAG TGCTGCAGAT CCGATACGCT AAGCTGGGTG ACACAGGTCG GTACACCTGC
    ATTGCATCAA CCCCCAGTGG TGAAGCAACA TGGAGTGCTT ACATTGAAGT CCAAGAATTT
    GGAGTTCCAG TTCAGCCTCC ACGACCCACC GACCCAAATT TAATCCCTAG TGCCCCCTCA
    AAACCTGAAG TTACAGATGT CAGCAGAAAT ACAGTAACGT TGTCATGGCA ACCAAATTTG
    AATTCAGGTG CAACTCCAAC ATCTTATATT ATAGAAGCCT TCAGCCATGC ATCTGGTAGC
    AGCTGGCAGA CCGTAGCAGA GAATGTGAAA ACAGAAACAT TTGCCATTAA AGGACTCAAA
    CCTAATGCAA TTTACCTTTT CCTTGTGAGG GCAGCAAATG CCTATGGAAT TAGTGATCCA
    AGCCAGATAT CAGATCCAGT GAAAACACAA GATGTTCCCC CAACAAGTCA GGGAGTAGAT
    CACAAGCAGG TCCAGAGAGA ATTGGGAAAC GTCGTACTGC ACCTTCACAA CCCCACCATC
    CTCTCATCCT CTTCCATCGA AGTGCACTGG ACAGTGGACC AACAATCTCA GTATATTCAA
    GGATATAAAA TTCTTTATCG GCCATCTGGA GGCAACCATG GTGAATCGGA GTGGTTAGTT
    TTTGAAGTGA GGACTCCAAC CAAAAATAGC GTGGTTATCC CTGATCTCAA AAAGGGAGTC
    AACTATGAAA TTAAGGCTCG TCCTTTTTTT AATGAATTTC AAGGAGCAGA TAGTGAAATT
    AAATTTGCCA AAACCTTAGA AGAAGCACCC AGTGCCCCAC CCCAAAGTGT AACCGTATCA
    AAGAATGATG GAAATGGAAC TGCAATTCTT GTTAGTTGGC AGCCACCTCC AGAAGACACT
    CAAAATGGAA TGGTCCAAGA GTATAAGGTT TGGTGTCTGG GCAATGAAAC TCGTTATCAC
    ATAAACAAGA CGGTGGATGG TTCCACCTTT TCTGTGGTCA TTCCTTCTCT GGTTCCTGGG
    ATCCGATACA GCGTAGAAGT AGCAGCCAGC ACGGGAGCTG GAGCTGGGGT GAAGAGCGAG
    CCACAGTTCA TCCAGTTAGA TTCTCATGGA AACCCCGTGT CACCTGAAGA CCAAGTCAGC
    CTTGCTCAGC AGATCTCAGA TGTTGTGAAG CAGCCAGCGT TCATTGCGGG AATCGGGGCC
    GCCTGCTGGA TCATCCTCAT GGTCTTTAGC ATCTGGCTCT ATCGACACCG GAAGAAGAGA
    AATGGACTGA CTAGTACTTA TGCGGGTATC AGAAAAGTCC CGTCTTTTAC CTTCACACCA
    ACAGTAACTT ATCAGAGAGG AGGTGAAGCT GTGAGCAGTG GAGGGAGGCC AGGACTTCTG
    AACATCAGCG AACCTGCCAC ACAGCCCTGG CTGGCAGACA CGTGGCCCAA TACTGGCAAC
    AATCACAATG ACTGCTCTAT CAATTGCTGT ACGGCAGGCA ATGGTAACAG TGATAACAAC
    CTGACCACCT ACAGTCGCCC AGCCGATTGT ATAGCAAATT ATAACAACCA ACTGGATAAC
    AAACAAACAA ATCTGATGCT CCCTGAGTCA ACTGTTTATG GTGATGTGGA CCTTAGTAAC
    AAAATCAATG AGATGAAAAC CTTCAATAGC CCAAATCTGA AGGATGGGCG TTTTGTCAAT
    CCATCAGGGC AGCCCACTCC TTATGCCACC ACCCAGCTCA TCCAGTCAAA CCTCAGCAAC
    AACATGAACA ATGGCAGCGG GGACTCCAGC GAGAAGCACT GGAAACCTCC CGGCCAGCAG
    AAACAAGAAG TGCCACCAGT TCAGTATAAC ATCATGGAGC AAAACAAACT GAACAAAGAT
    TATCGAGCAA ATGACACAGT TCCTCCAACT ATCCCATACA ACCAATCATA TGACCAGAAT
    ACAGGAGGAT CCTACAACAG TTCAGACCGG GGCAGTAGTA CATCTGGGAG TCAAGGGCAC
    AAGAAAGGGG CACGGACACC CAAAGTACCA AAACAAGGTG GCATGAACTG GGCAGACCTG
    CTTCCTCCTC CCCCAGCGCA TCCTCCTCCT CATAGCAATA GTGAAGAGTA CAATATTTCT
    GTAGATGAAA GCTATGACCA AGAAATGCCA TGTCCCGTGC CACCAGCAAG GATGTATTTG
    CAACAGAATG AACTAGAAGA GGAGGAAGAT GAGCGAGGCC CCACTCCCCC TGTGCGGGGA
    GCAGCTTCTT CTCCAGCTGC TGTGTCTTAT AGCCATCAGT CCACTGCTAC TCTGACCCCC
    TCCCCACAAG AAGAACTCCA GCCCATGTTG CAGGACTGCC CGGAGGAGAT TGGCCACATG
    CAGCACCAAC CCGATAGGAG ACGGCAGCCT GTGAGTCCTC CTCCACCACC AAGACCGATT
    TCCCCACCAC ATACCTATGG CTACATTTCA GGACCTCTTG TCTCAGATAT GGATACAGAT
    GCACCAGAAG AGGAAGAAGA TGAAGCAGAC ATGGAAGTGG CCAAGATGCA GACCAGACGG
    CTTTTGTTAC GTGGCCTTGA GCAGACCCCT GCCTCCAGTG TGGGGGACCT TGAAAGCTCT
    GTCACTGGGT CCATGATCAA TGGCTGGGGC TCAGCCTCAG AGGAGGACAA CATTTCCAGT
    GGACGCTCCA GTGTGAGTTC TTCTGATGGC TCCTTCTTCA CTGATGCTGA TTTTGCCCAG
    GCAGTGGCAG CAGCTGCAGA ATATGCTGGT CTTAAAGTGG CACGTCGTCA GATGCAGGAT
    GCTGCTGGTC GCCGACATTT TCATGCATCC CAGTGCCCTC GACCCACAAG TCCTGTCTCT
    ACAGACAGCA ACATGAGTGC TGCTGTAATG CAGAAGGCCA GACCAGCCAA GAAGCAAAAA
    CACCAGCCAG GACATCTGCG CAGAGAAGTC TACACAGATG ATCTACCACC TCCTCCGGTG
    CCACCACCTG CTATAAAATC ACCCACTGTC CAATCCAAGG CACAGCTGGA GGTGCGACCT
    GTTATGGTGC CAAAACTCCC TTCTATAGAA GCAAGAACAG ACCGATCATC AGATAGAAAA
    GTAGGCAGTT ACAAGGGGAG AGAAGTACTG GATGGAAGAC AGGTTGCTGA TATGAGAACC
    AATCCAGGTG ATACCAGAGA AGCACAGGAA CAGCAAAATG ATGGAAAAGG ACGAGTAAAC
    AAGGCAGCAA AACGAGACCT TCCACCAGCA AAGACTCACC TCATCCAAGA GGATATCCTA
    CCTTACTGTA GACCTACTTT TCCGACATCA AATAATCCCA GAGATCCTAG TTCTTCAAGC
    TCAATGTCAT CAAGAGGATC AGGAAGCAGA CAAAGAGAAC AAGCAAATGT AGGTCGAAGA
    AATATCGCAG AAATGCAAGT TCTTGGAGGA TATGAAAGAG GAGAAGATAA TAATGAAGAA
    TTAGAGGAAA CTGAAAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe283885
    Clone ID Related Accession (Same CDS sequence) XM_022413110.1
    Accession Version XM_022413110.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5052bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product roundabout homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCTGAGAC CTCCGTGCAG CTCAGTATGC TGCAGGAGGG GCAAGGAGGA AGTGTGCTGT 
    GTGTTGTCCC TGTGTGTTTT TCTGTCACAT GTGGATTTAC TTGTGTCTGG ACATGCTGTG
    ATCGAAGTGG CAACTACCCT TACTACTGTG ACCCCATCAG CAGCTCTGGT GTTGTTTCCG
    ACAGACCCTG AAGACCTAGA GAGGGGGAAC GACAACGGGA CGCCAATCCC CACCTCTGAT
    AATGATGACA ATTCGCTGGG CTACACAGGC TCCCGTCTCC GTCAGGAAGA TTTTCCACCT
    CGTATTGTTG AACACCCTTC AGACCTAATT GTGTCAAAAG GAGAACCTGC AACTCTGAAC
    TGTAAAGCTG AAGGCCGCCC CACGCCCACC ATTGAATGGT ACAAAGGTGG GGAGAGAGTG
    GAGACAGACA AAGATGACCC TCGCTCACAC CGAATGTTGC TGCCAAGTGG ATCTTTATTT
    TTCTTACGTA TAGTACATGG ACGAAAAAGT AGGCCTGATG AAGGGGTCTA CGTCTGTGTG
    GCACGGAATT ACCTCGGAGA GGCTGTGAGC CATAATGCAT CGCTGGAGGT GGCTATACTA
    CGGGATGACT TCAGACAAAA TCCTTCTGAT GTCATGGTGG CAGTGGGGGA ACCTGCTGTG
    ATGGAATGCC AACCCCCACG AGGCCATCCT GAACCCACCA TTTCGTGGAA GAAAGATGGC
    TCTCCACTGG ATGATAAAGA TGAAAGAATA ACTATACGAG GTGGAAAGCT CATGATTACT
    TACACCCGCA AAAGTGATGC TGGCAAATAT GTTTGTGTTG GTACCAACAT GGTTGGGGAA
    CGTGAGAGTG AAGTAGCAGA GCTAACTGTC TTGGAGAGAC CATCATTTGT GAAGAGACCT
    AGTAACTTGG CTGTAACTGT GGATGACAGT GCAGAATTTA AATGTGAGGC TCGAGGTGAC
    CCTGTGCCTA CGGTACGATG GAGGAAAGAC GATGGAGAAC TGCCCAAATC CAGATACGAA
    ATCAGAGATG ATCATACTTT GAAGATTCGG AAAGTGATGG CTGGTGACAT GGGGTCATAT
    ACTTGTGTTG CAGAAAATAT GGTTGGAAAA GCTGAAGCAT CAGCTACTCT GACTGTCCAA
    GAACCTCCAC ATTTTGTTGT GAAACCTCGT GACCAGGTTG TTGCCTTGGG GCGAACTGTA
    ACATTTCAGT GTGAAGCAAC CGGAAATCCT CAACCAGCTA TTTTTTGGAG AAGAGAGGGG
    AGTCAGAATC TGCTTTTCTC ATATCAGCCG CCACAGTCAA CTAGCCGCTT TTCCGTCTCC
    CAGACTGGTG ATCTCACTAT TACTAATGTC CAGCGGTCTG ATGTTGGTTA TTATATCTGC
    CAGACTTTAA ATGTTGCCGG AAGTATCATC ACAAAAGCAT ATTTGGAAGT TACTGATGTG
    ATTGCAGACC GGCCTCCCCC AGTCATTCGA CAAGGTCCTG TGAATCAGAC TGTAGCAGTG
    GATGGTACTT TAGTGCTCAG CTGTGTGGCC ACAGGCAGTC CAATGCCCAC GATTCTCTGG
    AGAAAGGATG GAGTCCTTGT TTCAACCCAA GATTCTAGAA TCAAACAGCT GGAGACGGGA
    GTGCTGCAGA TCCGATACGC TAAGCTGGGT GACACAGGTC GGTACACCTG CATTGCATCA
    ACCCCCAGTG GTGAAGCAAC ATGGAGTGCT TACATTGAAG TCCAAGAATT TGGAGTTCCA
    GTTCAGCCTC CACGACCCAC CGACCCAAAT TTAATCCCTA GTGCCCCCTC AAAACCTGAA
    GTTACAGATG TCAGCAGAAA TACAGTAACG TTGTCATGGC AACCAAATTT GAATTCAGGT
    GCAACTCCAA CATCTTATAT TATAGAAGCC TTCAGCCATG CATCTGGTAG CAGCTGGCAG
    ACCGTAGCAG AGAATGTGAA AACAGAAACA TTTGCCATTA AAGGACTCAA ACCTAATGCA
    ATTTACCTTT TCCTTGTGAG GGCAGCAAAT GCCTATGGAA TTAGTGATCC AAGCCAGATA
    TCAGATCCAG TGAAAACACA AGATGTTCCC CCAACAAGTC AGGGAGTAGA TCACAAGCAG
    GTCCAGAGAG AATTGGGAAA CGTCGTACTG CACCTTCACA ACCCCACCAT CCTCTCATCC
    TCTTCCATCG AAGTGCACTG GACAGTGGAC CAACAATCTC AGTATATTCA AGGATATAAA
    ATTCTTTATC GGCCATCTGG AGGCAACCAT GGTGAATCGG AGTGGTTAGT TTTTGAAGTG
    AGGACTCCAA CCAAAAATAG CGTGGTTATC CCTGATCTCA AAAAGGGAGT CAACTATGAA
    ATTAAGGCTC GTCCTTTTTT TAATGAATTT CAAGGAGCAG ATAGTGAAAT TAAATTTGCC
    AAAACCTTAG AAGAAGCACC CAGTGCCCCA CCCCAAAGTG TAACCGTATC AAAGAATGAT
    GGAAATGGAA CTGCAATTCT TGTTAGTTGG CAGCCACCTC CAGAAGACAC TCAAAATGGA
    ATGGTCCAAG AGTATAAGGT TTGGTGTCTG GGCAATGAAA CTCGTTATCA CATAAACAAG
    ACGGTGGATG GTTCCACCTT TTCTGTGGTC ATTCCTTCTC TGGTTCCTGG GATCCGATAC
    AGCGTAGAAG TAGCAGCCAG CACGGGAGCT GGAGCTGGGG TGAAGAGCGA GCCACAGTTC
    ATCCAGTTAG ATTCTCATGG AAACCCCGTG TCACCTGAAG ACCAAGTCAG CCTTGCTCAG
    CAGATCTCAG ATGTTGTGAA GCAGCCAGCG TTCATTGCGG GAATCGGGGC CGCCTGCTGG
    ATCATCCTCA TGGTCTTTAG CATCTGGCTC TATCGACACC GGAAGAAGAG AAATGGACTG
    ACTAGTACTT ATGCGGGTAT CAGAAAAGTC CCGTCTTTTA CCTTCACACC AACAGTAACT
    TATCAGAGAG GAGGTGAAGC TGTGAGCAGT GGAGGGAGGC CAGGACTTCT GAACATCAGC
    GAACCTGCCA CACAGCCCTG GCTGGCAGAC ACGTGGCCCA ATACTGGCAA CAATCACAAT
    GACTGCTCTA TCAATTGCTG TACGGCAGGC AATGGTAACA GTGATAACAA CCTGACCACC
    TACAGTCGCC CAGCCGATTG TATAGCAAAT TATAACAACC AACTGGATAA CAAACAAACA
    AATCTGATGC TCCCTGAGTC AACTGTTTAT GGTGATGTGG ACCTTAGTAA CAAAATCAAT
    GAGATGAAAA CCTTCAATAG CCCAAATCTG AAGGATGGGC GTTTTGTCAA TCCATCAGGG
    CAGCCCACTC CTTATGCCAC CACCCAGCTC ATCCAGTCAA ACCTCAGCAA CAACATGAAC
    AATGGCAGCG GGGACTCCAG CGAGAAGCAC TGGAAACCTC CCGGCCAGCA GAAACAAGAA
    GTGCCACCAG TTCAGTATAA CATCATGGAG CAAAACAAAC TGAACAAAGA TTATCGAGCA
    AATGACACAG TTCCTCCAAC TATCCCATAC AACCAATCAT ATGACCAGAA TACAGGAGGA
    TCCTACAACA GTTCAGACCG GGGCAGTAGT ACATCTGGGA GTCAAGGGCA CAAGAAAGGG
    GCACGGACAC CCAAAGTACC AAAACAAGGT GGCATGAACT GGGCAGACCT GCTTCCTCCT
    CCCCCAGCGC ATCCTCCTCC TCATAGCAAT AGTGAAGAGT ACAATATTTC TGTAGATGAA
    AGCTATGACC AAGAAATGCC ATGTCCCGTG CCACCAGCAA GGATGTATTT GCAACAGAAT
    GAACTAGAAG AGGAGGAAGA TGAGCGAGGC CCCACTCCCC CTGTGCGGGG AGCAGCTTCT
    TCTCCAGCTG CTGTGTCTTA TAGCCATCAG TCCACTGCTA CTCTGACCCC CTCCCCACAA
    GAAGAACTCC AGCCCATGTT GCAGGACTGC CCGGAGGAGA TTGGCCACAT GCAGCACCAA
    CCCGATAGGA GACGGCAGCC TGTGAGTCCT CCTCCACCAC CAAGACCGAT TTCCCCACCA
    CATACCTATG GCTACATTTC AGGACCTCTT GTCTCAGATA TGGATACAGA TGCACCAGAA
    GAGGAAGAAG ATGAAGCAGA CATGGAAGTG GCCAAGATGC AGACCAGACG GCTTTTGTTA
    CGTGGCCTTG AGCAGACCCC TGCCTCCAGT GTGGGGGACC TTGAAAGCTC TGTCACTGGG
    TCCATGATCA ATGGCTGGGG CTCAGCCTCA GAGGAGGACA ACATTTCCAG TGGACGCTCC
    AGTGTGAGTT CTTCTGATGG CTCCTTCTTC ACTGATGCTG ATTTTGCCCA GGCAGTGGCA
    GCAGCTGCAG AATATGCTGG TCTTAAAGTG GCACGTCGTC AGATGCAGGA TGCTGCTGGT
    CGCCGACATT TTCATGCATC CCAGTGCCCT CGACCCACAA GTCCTGTCTC TACAGACAGC
    AACATGAGTG CTGCTGTAAT GCAGAAGGCC AGACCAGCCA AGAAGCAAAA ACACCAGCCA
    GGACATCTGC GCAGAGAAGT CTACACAGAT GATCTACCAC CTCCTCCGGT GCCACCACCT
    GCTATAAAAT CACCCACTGT CCAATCCAAG GCACAGCTGG AGGTGCGACC TGTTATGGTG
    CCAAAACTCC CTTCTATAGA AGCAAGAACA GACCGATCAT CAGATAGAAA AGTAGGCAGT
    TACAAGGGGA GAGAAGTACT GGATGGAAGA CAGGTTGCTG ATATGAGAAC CAATCCAGGT
    GATACCAGAG AAGCACAGGA ACAGCAAAAT GATGGAAAAG GACGAGTAAA CAAGGCAGCA
    AAACGAGACC TTCCACCAGC AAAGACTCAC CTCATCCAAG AGGATATCCT ACCTTACTGT
    AGACCTACTT TTCCGACATC AAATAATCCC AGAGATCCTA GTTCTTCAAG CTCAATGTCA
    TCAAGAGGAT CAGGAAGCAG ACAAAGAGAA CAAGCAAATG TAGGTCGAAG AAATATCGCA
    GAAATGCAAG TTCTTGGAGG ATATGAAAGA GGAGAAGATA ATAATGAAGA ATTAGAGGAA
    ACTGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268818.1
    CDS16..5067
    Translation

    Target ORF information:

    RefSeq Version XM_022413110.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413110.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCTGAGAC CTCCGTGCAG CTCAGTATGC TGCAGGAGGG GCAAGGAGGA AGTGTGCTGT 
    GTGTTGTCCC TGTGTGTTTT TCTGTCACAT GTGGATTTAC TTGTGTCTGG ACATGCTGTG
    ATCGAAGTGG CAACTACCCT TACTACTGTG ACCCCATCAG CAGCTCTGGT GTTGTTTCCG
    ACAGACCCTG AAGACCTAGA GAGGGGGAAC GACAACGGGA CGCCAATCCC CACCTCTGAT
    AATGATGACA ATTCGCTGGG CTACACAGGC TCCCGTCTCC GTCAGGAAGA TTTTCCACCT
    CGTATTGTTG AACACCCTTC AGACCTAATT GTGTCAAAAG GAGAACCTGC AACTCTGAAC
    TGTAAAGCTG AAGGCCGCCC CACGCCCACC ATTGAATGGT ACAAAGGTGG GGAGAGAGTG
    GAGACAGACA AAGATGACCC TCGCTCACAC CGAATGTTGC TGCCAAGTGG ATCTTTATTT
    TTCTTACGTA TAGTACATGG ACGAAAAAGT AGGCCTGATG AAGGGGTCTA CGTCTGTGTG
    GCACGGAATT ACCTCGGAGA GGCTGTGAGC CATAATGCAT CGCTGGAGGT GGCTATACTA
    CGGGATGACT TCAGACAAAA TCCTTCTGAT GTCATGGTGG CAGTGGGGGA ACCTGCTGTG
    ATGGAATGCC AACCCCCACG AGGCCATCCT GAACCCACCA TTTCGTGGAA GAAAGATGGC
    TCTCCACTGG ATGATAAAGA TGAAAGAATA ACTATACGAG GTGGAAAGCT CATGATTACT
    TACACCCGCA AAAGTGATGC TGGCAAATAT GTTTGTGTTG GTACCAACAT GGTTGGGGAA
    CGTGAGAGTG AAGTAGCAGA GCTAACTGTC TTGGAGAGAC CATCATTTGT GAAGAGACCT
    AGTAACTTGG CTGTAACTGT GGATGACAGT GCAGAATTTA AATGTGAGGC TCGAGGTGAC
    CCTGTGCCTA CGGTACGATG GAGGAAAGAC GATGGAGAAC TGCCCAAATC CAGATACGAA
    ATCAGAGATG ATCATACTTT GAAGATTCGG AAAGTGATGG CTGGTGACAT GGGGTCATAT
    ACTTGTGTTG CAGAAAATAT GGTTGGAAAA GCTGAAGCAT CAGCTACTCT GACTGTCCAA
    GAACCTCCAC ATTTTGTTGT GAAACCTCGT GACCAGGTTG TTGCCTTGGG GCGAACTGTA
    ACATTTCAGT GTGAAGCAAC CGGAAATCCT CAACCAGCTA TTTTTTGGAG AAGAGAGGGG
    AGTCAGAATC TGCTTTTCTC ATATCAGCCG CCACAGTCAA CTAGCCGCTT TTCCGTCTCC
    CAGACTGGTG ATCTCACTAT TACTAATGTC CAGCGGTCTG ATGTTGGTTA TTATATCTGC
    CAGACTTTAA ATGTTGCCGG AAGTATCATC ACAAAAGCAT ATTTGGAAGT TACTGATGTG
    ATTGCAGACC GGCCTCCCCC AGTCATTCGA CAAGGTCCTG TGAATCAGAC TGTAGCAGTG
    GATGGTACTT TAGTGCTCAG CTGTGTGGCC ACAGGCAGTC CAATGCCCAC GATTCTCTGG
    AGAAAGGATG GAGTCCTTGT TTCAACCCAA GATTCTAGAA TCAAACAGCT GGAGACGGGA
    GTGCTGCAGA TCCGATACGC TAAGCTGGGT GACACAGGTC GGTACACCTG CATTGCATCA
    ACCCCCAGTG GTGAAGCAAC ATGGAGTGCT TACATTGAAG TCCAAGAATT TGGAGTTCCA
    GTTCAGCCTC CACGACCCAC CGACCCAAAT TTAATCCCTA GTGCCCCCTC AAAACCTGAA
    GTTACAGATG TCAGCAGAAA TACAGTAACG TTGTCATGGC AACCAAATTT GAATTCAGGT
    GCAACTCCAA CATCTTATAT TATAGAAGCC TTCAGCCATG CATCTGGTAG CAGCTGGCAG
    ACCGTAGCAG AGAATGTGAA AACAGAAACA TTTGCCATTA AAGGACTCAA ACCTAATGCA
    ATTTACCTTT TCCTTGTGAG GGCAGCAAAT GCCTATGGAA TTAGTGATCC AAGCCAGATA
    TCAGATCCAG TGAAAACACA AGATGTTCCC CCAACAAGTC AGGGAGTAGA TCACAAGCAG
    GTCCAGAGAG AATTGGGAAA CGTCGTACTG CACCTTCACA ACCCCACCAT CCTCTCATCC
    TCTTCCATCG AAGTGCACTG GACAGTGGAC CAACAATCTC AGTATATTCA AGGATATAAA
    ATTCTTTATC GGCCATCTGG AGGCAACCAT GGTGAATCGG AGTGGTTAGT TTTTGAAGTG
    AGGACTCCAA CCAAAAATAG CGTGGTTATC CCTGATCTCA AAAAGGGAGT CAACTATGAA
    ATTAAGGCTC GTCCTTTTTT TAATGAATTT CAAGGAGCAG ATAGTGAAAT TAAATTTGCC
    AAAACCTTAG AAGAAGCACC CAGTGCCCCA CCCCAAAGTG TAACCGTATC AAAGAATGAT
    GGAAATGGAA CTGCAATTCT TGTTAGTTGG CAGCCACCTC CAGAAGACAC TCAAAATGGA
    ATGGTCCAAG AGTATAAGGT TTGGTGTCTG GGCAATGAAA CTCGTTATCA CATAAACAAG
    ACGGTGGATG GTTCCACCTT TTCTGTGGTC ATTCCTTCTC TGGTTCCTGG GATCCGATAC
    AGCGTAGAAG TAGCAGCCAG CACGGGAGCT GGAGCTGGGG TGAAGAGCGA GCCACAGTTC
    ATCCAGTTAG ATTCTCATGG AAACCCCGTG TCACCTGAAG ACCAAGTCAG CCTTGCTCAG
    CAGATCTCAG ATGTTGTGAA GCAGCCAGCG TTCATTGCGG GAATCGGGGC CGCCTGCTGG
    ATCATCCTCA TGGTCTTTAG CATCTGGCTC TATCGACACC GGAAGAAGAG AAATGGACTG
    ACTAGTACTT ATGCGGGTAT CAGAAAAGTC CCGTCTTTTA CCTTCACACC AACAGTAACT
    TATCAGAGAG GAGGTGAAGC TGTGAGCAGT GGAGGGAGGC CAGGACTTCT GAACATCAGC
    GAACCTGCCA CACAGCCCTG GCTGGCAGAC ACGTGGCCCA ATACTGGCAA CAATCACAAT
    GACTGCTCTA TCAATTGCTG TACGGCAGGC AATGGTAACA GTGATAACAA CCTGACCACC
    TACAGTCGCC CAGCCGATTG TATAGCAAAT TATAACAACC AACTGGATAA CAAACAAACA
    AATCTGATGC TCCCTGAGTC AACTGTTTAT GGTGATGTGG ACCTTAGTAA CAAAATCAAT
    GAGATGAAAA CCTTCAATAG CCCAAATCTG AAGGATGGGC GTTTTGTCAA TCCATCAGGG
    CAGCCCACTC CTTATGCCAC CACCCAGCTC ATCCAGTCAA ACCTCAGCAA CAACATGAAC
    AATGGCAGCG GGGACTCCAG CGAGAAGCAC TGGAAACCTC CCGGCCAGCA GAAACAAGAA
    GTGCCACCAG TTCAGTATAA CATCATGGAG CAAAACAAAC TGAACAAAGA TTATCGAGCA
    AATGACACAG TTCCTCCAAC TATCCCATAC AACCAATCAT ATGACCAGAA TACAGGAGGA
    TCCTACAACA GTTCAGACCG GGGCAGTAGT ACATCTGGGA GTCAAGGGCA CAAGAAAGGG
    GCACGGACAC CCAAAGTACC AAAACAAGGT GGCATGAACT GGGCAGACCT GCTTCCTCCT
    CCCCCAGCGC ATCCTCCTCC TCATAGCAAT AGTGAAGAGT ACAATATTTC TGTAGATGAA
    AGCTATGACC AAGAAATGCC ATGTCCCGTG CCACCAGCAA GGATGTATTT GCAACAGAAT
    GAACTAGAAG AGGAGGAAGA TGAGCGAGGC CCCACTCCCC CTGTGCGGGG AGCAGCTTCT
    TCTCCAGCTG CTGTGTCTTA TAGCCATCAG TCCACTGCTA CTCTGACCCC CTCCCCACAA
    GAAGAACTCC AGCCCATGTT GCAGGACTGC CCGGAGGAGA TTGGCCACAT GCAGCACCAA
    CCCGATAGGA GACGGCAGCC TGTGAGTCCT CCTCCACCAC CAAGACCGAT TTCCCCACCA
    CATACCTATG GCTACATTTC AGGACCTCTT GTCTCAGATA TGGATACAGA TGCACCAGAA
    GAGGAAGAAG ATGAAGCAGA CATGGAAGTG GCCAAGATGC AGACCAGACG GCTTTTGTTA
    CGTGGCCTTG AGCAGACCCC TGCCTCCAGT GTGGGGGACC TTGAAAGCTC TGTCACTGGG
    TCCATGATCA ATGGCTGGGG CTCAGCCTCA GAGGAGGACA ACATTTCCAG TGGACGCTCC
    AGTGTGAGTT CTTCTGATGG CTCCTTCTTC ACTGATGCTG ATTTTGCCCA GGCAGTGGCA
    GCAGCTGCAG AATATGCTGG TCTTAAAGTG GCACGTCGTC AGATGCAGGA TGCTGCTGGT
    CGCCGACATT TTCATGCATC CCAGTGCCCT CGACCCACAA GTCCTGTCTC TACAGACAGC
    AACATGAGTG CTGCTGTAAT GCAGAAGGCC AGACCAGCCA AGAAGCAAAA ACACCAGCCA
    GGACATCTGC GCAGAGAAGT CTACACAGAT GATCTACCAC CTCCTCCGGT GCCACCACCT
    GCTATAAAAT CACCCACTGT CCAATCCAAG GCACAGCTGG AGGTGCGACC TGTTATGGTG
    CCAAAACTCC CTTCTATAGA AGCAAGAACA GACCGATCAT CAGATAGAAA AGTAGGCAGT
    TACAAGGGGA GAGAAGTACT GGATGGAAGA CAGGTTGCTG ATATGAGAAC CAATCCAGGT
    GATACCAGAG AAGCACAGGA ACAGCAAAAT GATGGAAAAG GACGAGTAAA CAAGGCAGCA
    AAACGAGACC TTCCACCAGC AAAGACTCAC CTCATCCAAG AGGATATCCT ACCTTACTGT
    AGACCTACTT TTCCGACATC AAATAATCCC AGAGATCCTA GTTCTTCAAG CTCAATGTCA
    TCAAGAGGAT CAGGAAGCAG ACAAAGAGAA CAAGCAAATG TAGGTCGAAG AAATATCGCA
    GAAATGCAAG TTCTTGGAGG ATATGAAAGA GGAGAAGATA ATAATGAAGA ATTAGAGGAA
    ACTGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe283886
    Clone ID Related Accession (Same CDS sequence) XM_022413111.1
    Accession Version XM_022413111.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5034bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product roundabout homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTGAGAC CTCCGTGCAG CTCAGTATGC TGCAGGAGGG GCAAGGAGGA AGTGTGCTGT 
    GTGTTGTCCC TGTGTGTTTT TCTGTCACAT GTGGATTTAC TTGTGTCTGG ACATGCTGTG
    ATCGAAGTGG CAACTACCCT TACTACTGTG ACCCCATCAG CAGCTCTGGT GTTGTTTCCG
    ACAGACCCTG AAGACCTAGA GAGGGGGAAC GACAACGGGA CGCCAATCCC CACCTCTGAT
    AATGATGACA ATTCGCTGGG CTACACAGGC TCCCGTCTCC GTCAGGAAGA TTTTCCACCT
    CGTATTGTTG AACACCCTTC AGACCTAATT GTGTCAAAAG GAGAACCTGC AACTCTGAAC
    TGTAAAGCTG AAGGCCGCCC CACGCCCACC ATTGAATGGT ACAAAGGTGG GGAGAGAGTG
    GAGACAGACA AAGATGACCC TCGCTCACAC CGAATGTTGC TGCCAAGTGG ATCTTTATTT
    TTCTTACGTA TAGTACATGG ACGAAAAAGT AGGCCTGATG AAGGGGTCTA CGTCTGTGTG
    GCACGGAATT ACCTCGGAGA GGCTGTGAGC CATAATGCAT CGCTGGAGGT GGCTATACTA
    CGGGATGACT TCAGACAAAA TCCTTCTGAT GTCATGGTGG CAGTGGGGGA ACCTGCTGTG
    ATGGAATGCC AACCCCCACG AGGCCATCCT GAACCCACCA TTTCGTGGAA GAAAGATGGC
    TCTCCACTGG ATGATAAAGA TGAAAGAATA ACTATACGAG GTGGAAAGCT CATGATTACT
    TACACCCGCA AAAGTGATGC TGGCAAATAT GTTTGTGTTG GTACCAACAT GGTTGGGGAA
    CGTGAGAGTG AAGTAGCAGA GCTAACTGTC TTGGAGAGAC CATCATTTGT GAAGAGACCT
    AGTAACTTGG CTGTAACTGT GGATGACAGT GCAGAATTTA AATGTGAGGC TCGAGGTGAC
    CCTGTGCCTA CGGTACGATG GAGGAAAGAC GATGGAGAAC TGCCCAAATC CAGATACGAA
    ATCAGAGATG ATCATACTTT GAAGATTCGG AAAGTGATGG CTGGTGACAT GGGGTCATAT
    ACTTGTGTTG CAGAAAATAT GGTTGGAAAA GCTGAAGCAT CAGCTACTCT GACTGTCCAA
    GTTGGGTCTG AACCTCCACA TTTTGTTGTG AAACCTCGTG ACCAGGTTGT TGCCTTGGGG
    CGAACTGTAA CATTTCAGTG TGAAGCAACC GGAAATCCTC AACCAGCTAT TTTTTGGAGA
    AGAGAGGGGA GTCAGAATCT GCTTTTCTCA TATCAGCCGC CACAGTCAAC TAGCCGCTTT
    TCCGTCTCCC AGACTGGTGA TCTCACTATT ACTAATGTCC AGCGGTCTGA TGTTGGTTAT
    TATATCTGCC AGACTTTAAA TGTTGCCGGA AGTATCATCA CAAAAGCATA TTTGGAAGTT
    ACTGATGTGA TTGCAGACCG GCCTCCCCCA GTCATTCGAC AAGGTCCTGT GAATCAGACT
    GTAGCAGTGG ATGGTACTTT AGTGCTCAGC TGTGTGGCCA CAGGCAGTCC AATGCCCACG
    ATTCTCTGGA GAAAGGATGG AGTCCTTGTT TCAACCCAAG ATTCTAGAAT CAAACAGCTG
    GAGACGGGAG TGCTGCAGAT CCGATACGCT AAGCTGGGTG ACACAGGTCG GTACACCTGC
    ATTGCATCAA CCCCCAGTGG TGAAGCAACA TGGAGTGCTT ACATTGAAGT CCAAGAATTT
    GGAGTTCCAG TTCAGCCTCC ACGACCCACC GACCCAAATT TAATCCCTAG TGCCCCCTCA
    AAACCTGAAG TTACAGATGT CAGCAGAAAT ACAGTAACGT TGTCATGGCA ACCAAATTTG
    AATTCAGGTG CAACTCCAAC ATCTTATATT ATAGAAGCCT TCAGCCATGC ATCTGGTAGC
    AGCTGGCAGA CCGTAGCAGA GAATGTGAAA ACAGAAACAT TTGCCATTAA AGGACTCAAA
    CCTAATGCAA TTTACCTTTT CCTTGTGAGG GCAGCAAATG CCTATGGAAT TAGTGATCCA
    AGCCAGATAT CAGATCCAGT GAAAACACAA GATGTTCCCC CAACAAGTCA GGGAGTAGAT
    CACAAGCAGG TCCAGAGAGA ATTGGGAAAC GTCGTACTGC ACCTTCACAA CCCCACCATC
    CTCTCATCCT CTTCCATCGA AGTGCACTGG ACAGTGGACC AACAATCTCA GTATATTCAA
    GGATATAAAA TTCTTTATCG GCCATCTGGA GGCAACCATG GTGAATCGGA GTGGTTAGTT
    TTTGAAGTGA GGACTCCAAC CAAAAATAGC GTGGTTATCC CTGATCTCAA AAAGGGAGTC
    AACTATGAAA TTAAGGCTCG TCCTTTTTTT AATGAATTTC AAGGAGCAGA TAGTGAAATT
    AAATTTGCCA AAACCTTAGA AGAAGCACCC AGTGCCCCAC CCCAAAGTGT AACCGTATCA
    AAGAATGATG GAAATGGAAC TGCAATTCTT GTTAGTTGGC AGCCACCTCC AGAAGACACT
    CAAAATGGAA TGGTCCAAGA GTATAAGGTT TGGTGTCTGG GCAATGAAAC TCGTTATCAC
    ATAAACAAGA CGGTGGATGG TTCCACCTTT TCTGTGGTCA TTCCTTCTCT GGTTCCTGGG
    ATCCGATACA GCGTAGAAGT AGCAGCCAGC ACGGGAGCTG GAGCTGGGGT GAAGAGCGAG
    CCACAGTTCA TCCAGTTAGA TTCTCATGGA AACCCCGTGT CACCTGAAGA CCAAGTCAGC
    CTTGCTCAGC AGATCTCAGA TGTTGTGAAG CAGCCAGCGT TCATTGCGGG AATCGGGGCC
    GCCTGCTGGA TCATCCTCAT GGTCTTTAGC ATCTGGCTCT ATCGACACCG GAAGAAGAGA
    AATGGACTGA CTAGTACTTA TGCGGGTATC AGAAAAGTAA CTTATCAGAG AGGAGGTGAA
    GCTGTGAGCA GTGGAGGGAG GCCAGGACTT CTGAACATCA GCGAACCTGC CACACAGCCC
    TGGCTGGCAG ACACGTGGCC CAATACTGGC AACAATCACA ATGACTGCTC TATCAATTGC
    TGTACGGCAG GCAATGGTAA CAGTGATAAC AACCTGACCA CCTACAGTCG CCCAGCCGAT
    TGTATAGCAA ATTATAACAA CCAACTGGAT AACAAACAAA CAAATCTGAT GCTCCCTGAG
    TCAACTGTTT ATGGTGATGT GGACCTTAGT AACAAAATCA ATGAGATGAA AACCTTCAAT
    AGCCCAAATC TGAAGGATGG GCGTTTTGTC AATCCATCAG GGCAGCCCAC TCCTTATGCC
    ACCACCCAGC TCATCCAGTC AAACCTCAGC AACAACATGA ACAATGGCAG CGGGGACTCC
    AGCGAGAAGC ACTGGAAACC TCCCGGCCAG CAGAAACAAG AAGTGCCACC AGTTCAGTAT
    AACATCATGG AGCAAAACAA ACTGAACAAA GATTATCGAG CAAATGACAC AGTTCCTCCA
    ACTATCCCAT ACAACCAATC ATATGACCAG AATACAGGAG GATCCTACAA CAGTTCAGAC
    CGGGGCAGTA GTACATCTGG GAGTCAAGGG CACAAGAAAG GGGCACGGAC ACCCAAAGTA
    CCAAAACAAG GTGGCATGAA CTGGGCAGAC CTGCTTCCTC CTCCCCCAGC GCATCCTCCT
    CCTCATAGCA ATAGTGAAGA GTACAATATT TCTGTAGATG AAAGCTATGA CCAAGAAATG
    CCATGTCCCG TGCCACCAGC AAGGATGTAT TTGCAACAGA ATGAACTAGA AGAGGAGGAA
    GATGAGCGAG GCCCCACTCC CCCTGTGCGG GGAGCAGCTT CTTCTCCAGC TGCTGTGTCT
    TATAGCCATC AGTCCACTGC TACTCTGACC CCCTCCCCAC AAGAAGAACT CCAGCCCATG
    TTGCAGGACT GCCCGGAGGA GATTGGCCAC ATGCAGCACC AACCCGATAG GAGACGGCAG
    CCTGTGAGTC CTCCTCCACC ACCAAGACCG ATTTCCCCAC CACATACCTA TGGCTACATT
    TCAGGACCTC TTGTCTCAGA TATGGATACA GATGCACCAG AAGAGGAAGA AGATGAAGCA
    GACATGGAAG TGGCCAAGAT GCAGACCAGA CGGCTTTTGT TACGTGGCCT TGAGCAGACC
    CCTGCCTCCA GTGTGGGGGA CCTTGAAAGC TCTGTCACTG GGTCCATGAT CAATGGCTGG
    GGCTCAGCCT CAGAGGAGGA CAACATTTCC AGTGGACGCT CCAGTGTGAG TTCTTCTGAT
    GGCTCCTTCT TCACTGATGC TGATTTTGCC CAGGCAGTGG CAGCAGCTGC AGAATATGCT
    GGTCTTAAAG TGGCACGTCG TCAGATGCAG GATGCTGCTG GTCGCCGACA TTTTCATGCA
    TCCCAGTGCC CTCGACCCAC AAGTCCTGTC TCTACAGACA GCAACATGAG TGCTGCTGTA
    ATGCAGAAGG CCAGACCAGC CAAGAAGCAA AAACACCAGC CAGGACATCT GCGCAGAGAA
    GTCTACACAG ATGATCTACC ACCTCCTCCG GTGCCACCAC CTGCTATAAA ATCACCCACT
    GTCCAATCCA AGGCACAGCT GGAGGTGCGA CCTGTTATGG TGCCAAAACT CCCTTCTATA
    GAAGCAAGAA CAGACCGATC ATCAGATAGA AAAGTAGGCA GTTACAAGGG GAGAGAAGTA
    CTGGATGGAA GACAGGTTGC TGATATGAGA ACCAATCCAG GTGATACCAG AGAAGCACAG
    GAACAGCAAA ATGATGGAAA AGGACGAGTA AACAAGGCAG CAAAACGAGA CCTTCCACCA
    GCAAAGACTC ACCTCATCCA AGAGGATATC CTACCTTACT GTAGACCTAC TTTTCCGACA
    TCAAATAATC CCAGAGATCC TAGTTCTTCA AGCTCAATGT CATCAAGAGG ATCAGGAAGC
    AGACAAAGAG AACAAGCAAA TGTAGGTCGA AGAAATATCG CAGAAATGCA AGTTCTTGGA
    GGATATGAAA GAGGAGAAGA TAATAATGAA GAATTAGAGG AAACTGAAAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268819.1
    CDS16..5049
    Translation

    Target ORF information:

    RefSeq Version XM_022413111.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413111.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTGAGAC CTCCGTGCAG CTCAGTATGC TGCAGGAGGG GCAAGGAGGA AGTGTGCTGT 
    GTGTTGTCCC TGTGTGTTTT TCTGTCACAT GTGGATTTAC TTGTGTCTGG ACATGCTGTG
    ATCGAAGTGG CAACTACCCT TACTACTGTG ACCCCATCAG CAGCTCTGGT GTTGTTTCCG
    ACAGACCCTG AAGACCTAGA GAGGGGGAAC GACAACGGGA CGCCAATCCC CACCTCTGAT
    AATGATGACA ATTCGCTGGG CTACACAGGC TCCCGTCTCC GTCAGGAAGA TTTTCCACCT
    CGTATTGTTG AACACCCTTC AGACCTAATT GTGTCAAAAG GAGAACCTGC AACTCTGAAC
    TGTAAAGCTG AAGGCCGCCC CACGCCCACC ATTGAATGGT ACAAAGGTGG GGAGAGAGTG
    GAGACAGACA AAGATGACCC TCGCTCACAC CGAATGTTGC TGCCAAGTGG ATCTTTATTT
    TTCTTACGTA TAGTACATGG ACGAAAAAGT AGGCCTGATG AAGGGGTCTA CGTCTGTGTG
    GCACGGAATT ACCTCGGAGA GGCTGTGAGC CATAATGCAT CGCTGGAGGT GGCTATACTA
    CGGGATGACT TCAGACAAAA TCCTTCTGAT GTCATGGTGG CAGTGGGGGA ACCTGCTGTG
    ATGGAATGCC AACCCCCACG AGGCCATCCT GAACCCACCA TTTCGTGGAA GAAAGATGGC
    TCTCCACTGG ATGATAAAGA TGAAAGAATA ACTATACGAG GTGGAAAGCT CATGATTACT
    TACACCCGCA AAAGTGATGC TGGCAAATAT GTTTGTGTTG GTACCAACAT GGTTGGGGAA
    CGTGAGAGTG AAGTAGCAGA GCTAACTGTC TTGGAGAGAC CATCATTTGT GAAGAGACCT
    AGTAACTTGG CTGTAACTGT GGATGACAGT GCAGAATTTA AATGTGAGGC TCGAGGTGAC
    CCTGTGCCTA CGGTACGATG GAGGAAAGAC GATGGAGAAC TGCCCAAATC CAGATACGAA
    ATCAGAGATG ATCATACTTT GAAGATTCGG AAAGTGATGG CTGGTGACAT GGGGTCATAT
    ACTTGTGTTG CAGAAAATAT GGTTGGAAAA GCTGAAGCAT CAGCTACTCT GACTGTCCAA
    GTTGGGTCTG AACCTCCACA TTTTGTTGTG AAACCTCGTG ACCAGGTTGT TGCCTTGGGG
    CGAACTGTAA CATTTCAGTG TGAAGCAACC GGAAATCCTC AACCAGCTAT TTTTTGGAGA
    AGAGAGGGGA GTCAGAATCT GCTTTTCTCA TATCAGCCGC CACAGTCAAC TAGCCGCTTT
    TCCGTCTCCC AGACTGGTGA TCTCACTATT ACTAATGTCC AGCGGTCTGA TGTTGGTTAT
    TATATCTGCC AGACTTTAAA TGTTGCCGGA AGTATCATCA CAAAAGCATA TTTGGAAGTT
    ACTGATGTGA TTGCAGACCG GCCTCCCCCA GTCATTCGAC AAGGTCCTGT GAATCAGACT
    GTAGCAGTGG ATGGTACTTT AGTGCTCAGC TGTGTGGCCA CAGGCAGTCC AATGCCCACG
    ATTCTCTGGA GAAAGGATGG AGTCCTTGTT TCAACCCAAG ATTCTAGAAT CAAACAGCTG
    GAGACGGGAG TGCTGCAGAT CCGATACGCT AAGCTGGGTG ACACAGGTCG GTACACCTGC
    ATTGCATCAA CCCCCAGTGG TGAAGCAACA TGGAGTGCTT ACATTGAAGT CCAAGAATTT
    GGAGTTCCAG TTCAGCCTCC ACGACCCACC GACCCAAATT TAATCCCTAG TGCCCCCTCA
    AAACCTGAAG TTACAGATGT CAGCAGAAAT ACAGTAACGT TGTCATGGCA ACCAAATTTG
    AATTCAGGTG CAACTCCAAC ATCTTATATT ATAGAAGCCT TCAGCCATGC ATCTGGTAGC
    AGCTGGCAGA CCGTAGCAGA GAATGTGAAA ACAGAAACAT TTGCCATTAA AGGACTCAAA
    CCTAATGCAA TTTACCTTTT CCTTGTGAGG GCAGCAAATG CCTATGGAAT TAGTGATCCA
    AGCCAGATAT CAGATCCAGT GAAAACACAA GATGTTCCCC CAACAAGTCA GGGAGTAGAT
    CACAAGCAGG TCCAGAGAGA ATTGGGAAAC GTCGTACTGC ACCTTCACAA CCCCACCATC
    CTCTCATCCT CTTCCATCGA AGTGCACTGG ACAGTGGACC AACAATCTCA GTATATTCAA
    GGATATAAAA TTCTTTATCG GCCATCTGGA GGCAACCATG GTGAATCGGA GTGGTTAGTT
    TTTGAAGTGA GGACTCCAAC CAAAAATAGC GTGGTTATCC CTGATCTCAA AAAGGGAGTC
    AACTATGAAA TTAAGGCTCG TCCTTTTTTT AATGAATTTC AAGGAGCAGA TAGTGAAATT
    AAATTTGCCA AAACCTTAGA AGAAGCACCC AGTGCCCCAC CCCAAAGTGT AACCGTATCA
    AAGAATGATG GAAATGGAAC TGCAATTCTT GTTAGTTGGC AGCCACCTCC AGAAGACACT
    CAAAATGGAA TGGTCCAAGA GTATAAGGTT TGGTGTCTGG GCAATGAAAC TCGTTATCAC
    ATAAACAAGA CGGTGGATGG TTCCACCTTT TCTGTGGTCA TTCCTTCTCT GGTTCCTGGG
    ATCCGATACA GCGTAGAAGT AGCAGCCAGC ACGGGAGCTG GAGCTGGGGT GAAGAGCGAG
    CCACAGTTCA TCCAGTTAGA TTCTCATGGA AACCCCGTGT CACCTGAAGA CCAAGTCAGC
    CTTGCTCAGC AGATCTCAGA TGTTGTGAAG CAGCCAGCGT TCATTGCGGG AATCGGGGCC
    GCCTGCTGGA TCATCCTCAT GGTCTTTAGC ATCTGGCTCT ATCGACACCG GAAGAAGAGA
    AATGGACTGA CTAGTACTTA TGCGGGTATC AGAAAAGTAA CTTATCAGAG AGGAGGTGAA
    GCTGTGAGCA GTGGAGGGAG GCCAGGACTT CTGAACATCA GCGAACCTGC CACACAGCCC
    TGGCTGGCAG ACACGTGGCC CAATACTGGC AACAATCACA ATGACTGCTC TATCAATTGC
    TGTACGGCAG GCAATGGTAA CAGTGATAAC AACCTGACCA CCTACAGTCG CCCAGCCGAT
    TGTATAGCAA ATTATAACAA CCAACTGGAT AACAAACAAA CAAATCTGAT GCTCCCTGAG
    TCAACTGTTT ATGGTGATGT GGACCTTAGT AACAAAATCA ATGAGATGAA AACCTTCAAT
    AGCCCAAATC TGAAGGATGG GCGTTTTGTC AATCCATCAG GGCAGCCCAC TCCTTATGCC
    ACCACCCAGC TCATCCAGTC AAACCTCAGC AACAACATGA ACAATGGCAG CGGGGACTCC
    AGCGAGAAGC ACTGGAAACC TCCCGGCCAG CAGAAACAAG AAGTGCCACC AGTTCAGTAT
    AACATCATGG AGCAAAACAA ACTGAACAAA GATTATCGAG CAAATGACAC AGTTCCTCCA
    ACTATCCCAT ACAACCAATC ATATGACCAG AATACAGGAG GATCCTACAA CAGTTCAGAC
    CGGGGCAGTA GTACATCTGG GAGTCAAGGG CACAAGAAAG GGGCACGGAC ACCCAAAGTA
    CCAAAACAAG GTGGCATGAA CTGGGCAGAC CTGCTTCCTC CTCCCCCAGC GCATCCTCCT
    CCTCATAGCA ATAGTGAAGA GTACAATATT TCTGTAGATG AAAGCTATGA CCAAGAAATG
    CCATGTCCCG TGCCACCAGC AAGGATGTAT TTGCAACAGA ATGAACTAGA AGAGGAGGAA
    GATGAGCGAG GCCCCACTCC CCCTGTGCGG GGAGCAGCTT CTTCTCCAGC TGCTGTGTCT
    TATAGCCATC AGTCCACTGC TACTCTGACC CCCTCCCCAC AAGAAGAACT CCAGCCCATG
    TTGCAGGACT GCCCGGAGGA GATTGGCCAC ATGCAGCACC AACCCGATAG GAGACGGCAG
    CCTGTGAGTC CTCCTCCACC ACCAAGACCG ATTTCCCCAC CACATACCTA TGGCTACATT
    TCAGGACCTC TTGTCTCAGA TATGGATACA GATGCACCAG AAGAGGAAGA AGATGAAGCA
    GACATGGAAG TGGCCAAGAT GCAGACCAGA CGGCTTTTGT TACGTGGCCT TGAGCAGACC
    CCTGCCTCCA GTGTGGGGGA CCTTGAAAGC TCTGTCACTG GGTCCATGAT CAATGGCTGG
    GGCTCAGCCT CAGAGGAGGA CAACATTTCC AGTGGACGCT CCAGTGTGAG TTCTTCTGAT
    GGCTCCTTCT TCACTGATGC TGATTTTGCC CAGGCAGTGG CAGCAGCTGC AGAATATGCT
    GGTCTTAAAG TGGCACGTCG TCAGATGCAG GATGCTGCTG GTCGCCGACA TTTTCATGCA
    TCCCAGTGCC CTCGACCCAC AAGTCCTGTC TCTACAGACA GCAACATGAG TGCTGCTGTA
    ATGCAGAAGG CCAGACCAGC CAAGAAGCAA AAACACCAGC CAGGACATCT GCGCAGAGAA
    GTCTACACAG ATGATCTACC ACCTCCTCCG GTGCCACCAC CTGCTATAAA ATCACCCACT
    GTCCAATCCA AGGCACAGCT GGAGGTGCGA CCTGTTATGG TGCCAAAACT CCCTTCTATA
    GAAGCAAGAA CAGACCGATC ATCAGATAGA AAAGTAGGCA GTTACAAGGG GAGAGAAGTA
    CTGGATGGAA GACAGGTTGC TGATATGAGA ACCAATCCAG GTGATACCAG AGAAGCACAG
    GAACAGCAAA ATGATGGAAA AGGACGAGTA AACAAGGCAG CAAAACGAGA CCTTCCACCA
    GCAAAGACTC ACCTCATCCA AGAGGATATC CTACCTTACT GTAGACCTAC TTTTCCGACA
    TCAAATAATC CCAGAGATCC TAGTTCTTCA AGCTCAATGT CATCAAGAGG ATCAGGAAGC
    AGACAAAGAG AACAAGCAAA TGTAGGTCGA AGAAATATCG CAGAAATGCA AGTTCTTGGA
    GGATATGAAA GAGGAGAAGA TAATAATGAA GAATTAGAGG AAACTGAAAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe283887
    Clone ID Related Accession (Same CDS sequence) XM_022413112.1
    Accession Version XM_022413112.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5022bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product roundabout homolog 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTGAGAC CTCCGTGCAG CTCAGTATGC TGCAGGAGGG GCAAGGAGGA AGTGTGCTGT 
    GTGTTGTCCC TGTGTGTTTT TCTGTCACAT GTGGATTTAC TTGTGTCTGG ACATGCTGTG
    ATCGAAGTGG CAACTACCCT TACTACTGTG ACCCCATCAG CAGCTCTGGT GTTGTTTCCG
    ACAGACCCTG AAGACCTAGA GAGGGGGAAC GACAACGGGA CGCCAATCCC CACCTCTGAT
    AATGATGACA ATTCGCTGGG CTACACAGGC TCCCGTCTCC GTCAGGAAGA TTTTCCACCT
    CGTATTGTTG AACACCCTTC AGACCTAATT GTGTCAAAAG GAGAACCTGC AACTCTGAAC
    TGTAAAGCTG AAGGCCGCCC CACGCCCACC ATTGAATGGT ACAAAGGTGG GGAGAGAGTG
    GAGACAGACA AAGATGACCC TCGCTCACAC CGAATGTTGC TGCCAAGTGG ATCTTTATTT
    TTCTTACGTA TAGTACATGG ACGAAAAAGT AGGCCTGATG AAGGGGTCTA CGTCTGTGTG
    GCACGGAATT ACCTCGGAGA GGCTGTGAGC CATAATGCAT CGCTGGAGGT GGCTATACTA
    CGGGATGACT TCAGACAAAA TCCTTCTGAT GTCATGGTGG CAGTGGGGGA ACCTGCTGTG
    ATGGAATGCC AACCCCCACG AGGCCATCCT GAACCCACCA TTTCGTGGAA GAAAGATGGC
    TCTCCACTGG ATGATAAAGA TGAAAGAATA ACTATACGAG GTGGAAAGCT CATGATTACT
    TACACCCGCA AAAGTGATGC TGGCAAATAT GTTTGTGTTG GTACCAACAT GGTTGGGGAA
    CGTGAGAGTG AAGTAGCAGA GCTAACTGTC TTGGAGAGAC CATCATTTGT GAAGAGACCT
    AGTAACTTGG CTGTAACTGT GGATGACAGT GCAGAATTTA AATGTGAGGC TCGAGGTGAC
    CCTGTGCCTA CGGTACGATG GAGGAAAGAC GATGGAGAAC TGCCCAAATC CAGATACGAA
    ATCAGAGATG ATCATACTTT GAAGATTCGG AAAGTGATGG CTGGTGACAT GGGGTCATAT
    ACTTGTGTTG CAGAAAATAT GGTTGGAAAA GCTGAAGCAT CAGCTACTCT GACTGTCCAA
    GTTGGGTCTG AACCTCCACA TTTTGTTGTG AAACCTCGTG ACCAGGTTGT TGCCTTGGGG
    CGAACTGTAA CATTTCAGTG TGAAGCAACC GGAAATCCTC AACCAGCTAT TTTTTGGAGA
    AGAGAGGGGA GTCAGAATCT GCTTTTCTCA TATCAGCCGC CACAGTCAAC TAGCCGCTTT
    TCCGTCTCCC AGACTGGTGA TCTCACTATT ACTAATGTCC AGCGGTCTGA TGTTGGTTAT
    TATATCTGCC AGACTTTAAA TGTTGCCGGA AGTATCATCA CAAAAGCATA TTTGGAAGTT
    ACTGATGTGA TTGCAGACCG GCCTCCCCCA GTCATTCGAC AAGGTCCTGT GAATCAGACT
    GTAGCAGTGG ATGGTACTTT AGTGCTCAGC TGTGTGGCCA CAGGCAGTCC AATGCCCACG
    ATTCTCTGGA GAAAGGATGG AGTCCTTGTT TCAACCCAAG ATTCTAGAAT CAAACAGCTG
    GAGACGGGAG TGCTGCAGAT CCGATACGCT AAGCTGGGTG ACACAGGTCG GTACACCTGC
    ATTGCATCAA CCCCCAGTGG TGAAGCAACA TGGAGTGCTT ACATTGAAGT CCAAGAATTT
    GGAGTTCCAG TTCAGCCTCC ACGACCCACC GACCCAAATT TAATCCCTAG TGCCCCCTCA
    AAACCTGAAG TTACAGATGT CAGCAGAAAT ACAGTAACGT TGTCATGGCA ACCAAATTTG
    AATTCAGGTG CAACTCCAAC ATCTTATATT ATAGAAGCCT TCAGCCATGC ATCTGGTAGC
    AGCTGGCAGA CCGTAGCAGA GAATGTGAAA ACAGAAACAT TTGCCATTAA AGGACTCAAA
    CCTAATGCAA TTTACCTTTT CCTTGTGAGG GCAGCAAATG CCTATGGAAT TAGTGATCCA
    AGCCAGATAT CAGATCCAGT GAAAACACAA GATGTTCCCC CAACAAGTCA GGGAGTAGAT
    CACAAGCAGG TCCAGAGAGA ATTGGGAAAC GTCGTACTGC ACCTTCACAA CCCCACCATC
    CTCTCATCCT CTTCCATCGA AGTGCACTGG ACAGTGGACC AACAATCTCA GTATATTCAA
    GGATATAAAA TTCTTTATCG GCCATCTGGA GGCAACCATG GTGAATCGGA GTGGTTAGTT
    TTTGAAGTGA GGACTCCAAC CAAAAATAGC GTGGTTATCC CTGATCTCAA AAAGGGAGTC
    AACTATGAAA TTAAGGCTCG TCCTTTTTTT AATGAATTTC AAGGAGCAGA TAGTGAAATT
    AAATTTGCCA AAACCTTAGA AGAAGCACCC AGTGCCCCAC CCCAAAGTGT AACCGTATCA
    AAGAATGATG GAAATGGAAC TGCAATTCTT GTTAGTTGGC AGCCACCTCC AGAAGACACT
    CAAAATGGAA TGGTCCAAGA GTATAAGGTT TGGTGTCTGG GCAATGAAAC TCGTTATCAC
    ATAAACAAGA CGGTGGATGG TTCCACCTTT TCTGTGGTCA TTCCTTCTCT GGTTCCTGGG
    ATCCGATACA GCGTAGAAGT AGCAGCCAGC ACGGGAGCTG GAGCTGGGGT GAAGAGCGAG
    CCACAGTTCA TCCAGTTAGA TTCTCATGGA AACCCCGTGT CACCTGAAGA CCAAGTCAGC
    CTTGCTCAGC AGATCTCAGA TGTTGTGAAG CAGCCAGCGT TCATTGCGGG AATCGGGGCC
    GCCTGCTGGA TCATCCTCAT GGTCTTTAGC ATCTGGCTCT ATCGACACCG GAAGAAGAGA
    AATGGACTGA CTAGTACTTA TGCGGGTATC AGAAAAGTCC CGTCTTTTAC CTTCACACCA
    ACAGTAACTT ATCAGAGAGG AGGTGAAGCT GTGAGCAGTG GAGGGAGGCC AGGACTTCTG
    AACATCAGCG AACCTGCCAC ACAGCCCTGG CTGGCAGACA CGTGGCCCAA TACTGGCAAC
    AATCACAATG ACTGCTCTAT CAATTGCTGT ACGGCAGGCA ATGCCGATTG TATAGCAAAT
    TATAACAACC AACTGGATAA CAAACAAACA AATCTGATGC TCCCTGAGTC AACTGTTTAT
    GGTGATGTGG ACCTTAGTAA CAAAATCAAT GAGATGAAAA CCTTCAATAG CCCAAATCTG
    AAGGATGGGC GTTTTGTCAA TCCATCAGGG CAGCCCACTC CTTATGCCAC CACCCAGCTC
    ATCCAGTCAA ACCTCAGCAA CAACATGAAC AATGGCAGCG GGGACTCCAG CGAGAAGCAC
    TGGAAACCTC CCGGCCAGCA GAAACAAGAA GTGCCACCAG TTCAGTATAA CATCATGGAG
    CAAAACAAAC TGAACAAAGA TTATCGAGCA AATGACACAG TTCCTCCAAC TATCCCATAC
    AACCAATCAT ATGACCAGAA TACAGGAGGA TCCTACAACA GTTCAGACCG GGGCAGTAGT
    ACATCTGGGA GTCAAGGGCA CAAGAAAGGG GCACGGACAC CCAAAGTACC AAAACAAGGT
    GGCATGAACT GGGCAGACCT GCTTCCTCCT CCCCCAGCGC ATCCTCCTCC TCATAGCAAT
    AGTGAAGAGT ACAATATTTC TGTAGATGAA AGCTATGACC AAGAAATGCC ATGTCCCGTG
    CCACCAGCAA GGATGTATTT GCAACAGAAT GAACTAGAAG AGGAGGAAGA TGAGCGAGGC
    CCCACTCCCC CTGTGCGGGG AGCAGCTTCT TCTCCAGCTG CTGTGTCTTA TAGCCATCAG
    TCCACTGCTA CTCTGACCCC CTCCCCACAA GAAGAACTCC AGCCCATGTT GCAGGACTGC
    CCGGAGGAGA TTGGCCACAT GCAGCACCAA CCCGATAGGA GACGGCAGCC TGTGAGTCCT
    CCTCCACCAC CAAGACCGAT TTCCCCACCA CATACCTATG GCTACATTTC AGGACCTCTT
    GTCTCAGATA TGGATACAGA TGCACCAGAA GAGGAAGAAG ATGAAGCAGA CATGGAAGTG
    GCCAAGATGC AGACCAGACG GCTTTTGTTA CGTGGCCTTG AGCAGACCCC TGCCTCCAGT
    GTGGGGGACC TTGAAAGCTC TGTCACTGGG TCCATGATCA ATGGCTGGGG CTCAGCCTCA
    GAGGAGGACA ACATTTCCAG TGGACGCTCC AGTGTGAGTT CTTCTGATGG CTCCTTCTTC
    ACTGATGCTG ATTTTGCCCA GGCAGTGGCA GCAGCTGCAG AATATGCTGG TCTTAAAGTG
    GCACGTCGTC AGATGCAGGA TGCTGCTGGT CGCCGACATT TTCATGCATC CCAGTGCCCT
    CGACCCACAA GTCCTGTCTC TACAGACAGC AACATGAGTG CTGCTGTAAT GCAGAAGGCC
    AGACCAGCCA AGAAGCAAAA ACACCAGCCA GGACATCTGC GCAGAGAAGT CTACACAGAT
    GATCTACCAC CTCCTCCGGT GCCACCACCT GCTATAAAAT CACCCACTGT CCAATCCAAG
    GCACAGCTGG AGGTGCGACC TGTTATGGTG CCAAAACTCC CTTCTATAGA AGCAAGAACA
    GACCGATCAT CAGATAGAAA AGTAGGCAGT TACAAGGGGA GAGAAGTACT GGATGGAAGA
    CAGGTTGCTG ATATGAGAAC CAATCCAGGT GATACCAGAG AAGCACAGGA ACAGCAAAAT
    GATGGAAAAG GACGAGTAAA CAAGGCAGCA AAACGAGACC TTCCACCAGC AAAGACTCAC
    CTCATCCAAG AGGATATCCT ACCTTACTGT AGACCTACTT TTCCGACATC AAATAATCCC
    AGAGATCCTA GTTCTTCAAG CTCAATGTCA TCAAGAGGAT CAGGAAGCAG ACAAAGAGAA
    CAAGCAAATG TAGGTCGAAG AAATATCGCA GAAATGCAAG TTCTTGGAGG ATATGAAAGA
    GGAGAAGATA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268820.1
    CDS16..5037
    Translation

    Target ORF information:

    RefSeq Version XM_022413112.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413112.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTGAGAC CTCCGTGCAG CTCAGTATGC TGCAGGAGGG GCAAGGAGGA AGTGTGCTGT 
    GTGTTGTCCC TGTGTGTTTT TCTGTCACAT GTGGATTTAC TTGTGTCTGG ACATGCTGTG
    ATCGAAGTGG CAACTACCCT TACTACTGTG ACCCCATCAG CAGCTCTGGT GTTGTTTCCG
    ACAGACCCTG AAGACCTAGA GAGGGGGAAC GACAACGGGA CGCCAATCCC CACCTCTGAT
    AATGATGACA ATTCGCTGGG CTACACAGGC TCCCGTCTCC GTCAGGAAGA TTTTCCACCT
    CGTATTGTTG AACACCCTTC AGACCTAATT GTGTCAAAAG GAGAACCTGC AACTCTGAAC
    TGTAAAGCTG AAGGCCGCCC CACGCCCACC ATTGAATGGT ACAAAGGTGG GGAGAGAGTG
    GAGACAGACA AAGATGACCC TCGCTCACAC CGAATGTTGC TGCCAAGTGG ATCTTTATTT
    TTCTTACGTA TAGTACATGG ACGAAAAAGT AGGCCTGATG AAGGGGTCTA CGTCTGTGTG
    GCACGGAATT ACCTCGGAGA GGCTGTGAGC CATAATGCAT CGCTGGAGGT GGCTATACTA
    CGGGATGACT TCAGACAAAA TCCTTCTGAT GTCATGGTGG CAGTGGGGGA ACCTGCTGTG
    ATGGAATGCC AACCCCCACG AGGCCATCCT GAACCCACCA TTTCGTGGAA GAAAGATGGC
    TCTCCACTGG ATGATAAAGA TGAAAGAATA ACTATACGAG GTGGAAAGCT CATGATTACT
    TACACCCGCA AAAGTGATGC TGGCAAATAT GTTTGTGTTG GTACCAACAT GGTTGGGGAA
    CGTGAGAGTG AAGTAGCAGA GCTAACTGTC TTGGAGAGAC CATCATTTGT GAAGAGACCT
    AGTAACTTGG CTGTAACTGT GGATGACAGT GCAGAATTTA AATGTGAGGC TCGAGGTGAC
    CCTGTGCCTA CGGTACGATG GAGGAAAGAC GATGGAGAAC TGCCCAAATC CAGATACGAA
    ATCAGAGATG ATCATACTTT GAAGATTCGG AAAGTGATGG CTGGTGACAT GGGGTCATAT
    ACTTGTGTTG CAGAAAATAT GGTTGGAAAA GCTGAAGCAT CAGCTACTCT GACTGTCCAA
    GTTGGGTCTG AACCTCCACA TTTTGTTGTG AAACCTCGTG ACCAGGTTGT TGCCTTGGGG
    CGAACTGTAA CATTTCAGTG TGAAGCAACC GGAAATCCTC AACCAGCTAT TTTTTGGAGA
    AGAGAGGGGA GTCAGAATCT GCTTTTCTCA TATCAGCCGC CACAGTCAAC TAGCCGCTTT
    TCCGTCTCCC AGACTGGTGA TCTCACTATT ACTAATGTCC AGCGGTCTGA TGTTGGTTAT
    TATATCTGCC AGACTTTAAA TGTTGCCGGA AGTATCATCA CAAAAGCATA TTTGGAAGTT
    ACTGATGTGA TTGCAGACCG GCCTCCCCCA GTCATTCGAC AAGGTCCTGT GAATCAGACT
    GTAGCAGTGG ATGGTACTTT AGTGCTCAGC TGTGTGGCCA CAGGCAGTCC AATGCCCACG
    ATTCTCTGGA GAAAGGATGG AGTCCTTGTT TCAACCCAAG ATTCTAGAAT CAAACAGCTG
    GAGACGGGAG TGCTGCAGAT CCGATACGCT AAGCTGGGTG ACACAGGTCG GTACACCTGC
    ATTGCATCAA CCCCCAGTGG TGAAGCAACA TGGAGTGCTT ACATTGAAGT CCAAGAATTT
    GGAGTTCCAG TTCAGCCTCC ACGACCCACC GACCCAAATT TAATCCCTAG TGCCCCCTCA
    AAACCTGAAG TTACAGATGT CAGCAGAAAT ACAGTAACGT TGTCATGGCA ACCAAATTTG
    AATTCAGGTG CAACTCCAAC ATCTTATATT ATAGAAGCCT TCAGCCATGC ATCTGGTAGC
    AGCTGGCAGA CCGTAGCAGA GAATGTGAAA ACAGAAACAT TTGCCATTAA AGGACTCAAA
    CCTAATGCAA TTTACCTTTT CCTTGTGAGG GCAGCAAATG CCTATGGAAT TAGTGATCCA
    AGCCAGATAT CAGATCCAGT GAAAACACAA GATGTTCCCC CAACAAGTCA GGGAGTAGAT
    CACAAGCAGG TCCAGAGAGA ATTGGGAAAC GTCGTACTGC ACCTTCACAA CCCCACCATC
    CTCTCATCCT CTTCCATCGA AGTGCACTGG ACAGTGGACC AACAATCTCA GTATATTCAA
    GGATATAAAA TTCTTTATCG GCCATCTGGA GGCAACCATG GTGAATCGGA GTGGTTAGTT
    TTTGAAGTGA GGACTCCAAC CAAAAATAGC GTGGTTATCC CTGATCTCAA AAAGGGAGTC
    AACTATGAAA TTAAGGCTCG TCCTTTTTTT AATGAATTTC AAGGAGCAGA TAGTGAAATT
    AAATTTGCCA AAACCTTAGA AGAAGCACCC AGTGCCCCAC CCCAAAGTGT AACCGTATCA
    AAGAATGATG GAAATGGAAC TGCAATTCTT GTTAGTTGGC AGCCACCTCC AGAAGACACT
    CAAAATGGAA TGGTCCAAGA GTATAAGGTT TGGTGTCTGG GCAATGAAAC TCGTTATCAC
    ATAAACAAGA CGGTGGATGG TTCCACCTTT TCTGTGGTCA TTCCTTCTCT GGTTCCTGGG
    ATCCGATACA GCGTAGAAGT AGCAGCCAGC ACGGGAGCTG GAGCTGGGGT GAAGAGCGAG
    CCACAGTTCA TCCAGTTAGA TTCTCATGGA AACCCCGTGT CACCTGAAGA CCAAGTCAGC
    CTTGCTCAGC AGATCTCAGA TGTTGTGAAG CAGCCAGCGT TCATTGCGGG AATCGGGGCC
    GCCTGCTGGA TCATCCTCAT GGTCTTTAGC ATCTGGCTCT ATCGACACCG GAAGAAGAGA
    AATGGACTGA CTAGTACTTA TGCGGGTATC AGAAAAGTCC CGTCTTTTAC CTTCACACCA
    ACAGTAACTT ATCAGAGAGG AGGTGAAGCT GTGAGCAGTG GAGGGAGGCC AGGACTTCTG
    AACATCAGCG AACCTGCCAC ACAGCCCTGG CTGGCAGACA CGTGGCCCAA TACTGGCAAC
    AATCACAATG ACTGCTCTAT CAATTGCTGT ACGGCAGGCA ATGCCGATTG TATAGCAAAT
    TATAACAACC AACTGGATAA CAAACAAACA AATCTGATGC TCCCTGAGTC AACTGTTTAT
    GGTGATGTGG ACCTTAGTAA CAAAATCAAT GAGATGAAAA CCTTCAATAG CCCAAATCTG
    AAGGATGGGC GTTTTGTCAA TCCATCAGGG CAGCCCACTC CTTATGCCAC CACCCAGCTC
    ATCCAGTCAA ACCTCAGCAA CAACATGAAC AATGGCAGCG GGGACTCCAG CGAGAAGCAC
    TGGAAACCTC CCGGCCAGCA GAAACAAGAA GTGCCACCAG TTCAGTATAA CATCATGGAG
    CAAAACAAAC TGAACAAAGA TTATCGAGCA AATGACACAG TTCCTCCAAC TATCCCATAC
    AACCAATCAT ATGACCAGAA TACAGGAGGA TCCTACAACA GTTCAGACCG GGGCAGTAGT
    ACATCTGGGA GTCAAGGGCA CAAGAAAGGG GCACGGACAC CCAAAGTACC AAAACAAGGT
    GGCATGAACT GGGCAGACCT GCTTCCTCCT CCCCCAGCGC ATCCTCCTCC TCATAGCAAT
    AGTGAAGAGT ACAATATTTC TGTAGATGAA AGCTATGACC AAGAAATGCC ATGTCCCGTG
    CCACCAGCAA GGATGTATTT GCAACAGAAT GAACTAGAAG AGGAGGAAGA TGAGCGAGGC
    CCCACTCCCC CTGTGCGGGG AGCAGCTTCT TCTCCAGCTG CTGTGTCTTA TAGCCATCAG
    TCCACTGCTA CTCTGACCCC CTCCCCACAA GAAGAACTCC AGCCCATGTT GCAGGACTGC
    CCGGAGGAGA TTGGCCACAT GCAGCACCAA CCCGATAGGA GACGGCAGCC TGTGAGTCCT
    CCTCCACCAC CAAGACCGAT TTCCCCACCA CATACCTATG GCTACATTTC AGGACCTCTT
    GTCTCAGATA TGGATACAGA TGCACCAGAA GAGGAAGAAG ATGAAGCAGA CATGGAAGTG
    GCCAAGATGC AGACCAGACG GCTTTTGTTA CGTGGCCTTG AGCAGACCCC TGCCTCCAGT
    GTGGGGGACC TTGAAAGCTC TGTCACTGGG TCCATGATCA ATGGCTGGGG CTCAGCCTCA
    GAGGAGGACA ACATTTCCAG TGGACGCTCC AGTGTGAGTT CTTCTGATGG CTCCTTCTTC
    ACTGATGCTG ATTTTGCCCA GGCAGTGGCA GCAGCTGCAG AATATGCTGG TCTTAAAGTG
    GCACGTCGTC AGATGCAGGA TGCTGCTGGT CGCCGACATT TTCATGCATC CCAGTGCCCT
    CGACCCACAA GTCCTGTCTC TACAGACAGC AACATGAGTG CTGCTGTAAT GCAGAAGGCC
    AGACCAGCCA AGAAGCAAAA ACACCAGCCA GGACATCTGC GCAGAGAAGT CTACACAGAT
    GATCTACCAC CTCCTCCGGT GCCACCACCT GCTATAAAAT CACCCACTGT CCAATCCAAG
    GCACAGCTGG AGGTGCGACC TGTTATGGTG CCAAAACTCC CTTCTATAGA AGCAAGAACA
    GACCGATCAT CAGATAGAAA AGTAGGCAGT TACAAGGGGA GAGAAGTACT GGATGGAAGA
    CAGGTTGCTG ATATGAGAAC CAATCCAGGT GATACCAGAG AAGCACAGGA ACAGCAAAAT
    GATGGAAAAG GACGAGTAAA CAAGGCAGCA AAACGAGACC TTCCACCAGC AAAGACTCAC
    CTCATCCAAG AGGATATCCT ACCTTACTGT AGACCTACTT TTCCGACATC AAATAATCCC
    AGAGATCCTA GTTCTTCAAG CTCAATGTCA TCAAGAGGAT CAGGAAGCAG ACAAAGAGAA
    CAAGCAAATG TAGGTCGAAG AAATATCGCA GAAATGCAAG TTCTTGGAGG ATATGAAAGA
    GGAGAAGATA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe283888
    Clone ID Related Accession (Same CDS sequence) XM_022413113.1
    Accession Version XM_022413113.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4896bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product roundabout homolog 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCTGAGAC CTCCGTGCAG CTCAGTATGC TGCAGGAGGG GCAAGGAGGA AGTGTGCTGT 
    GTGTTGTCCC TGTGTGTTTT TCTGTCACAT GTGGATTTAC TTGTGTCTGG ACATGCTGTG
    ATCGAAGTGG CAACTACCCT TACTACTGTG ACCCCATCAG CAGCTCTGGT GTTGTTTCCG
    ACAGACCCTG AAGACCTAGA GAGGGGGAAC GACAACGGGA CGCCAATCCC CACCTCTGAT
    AATGATGACA ATTCGCTGGG CTACACAGGC TCCCGTCTCC GTCAGGAAGA TTTTCCACCT
    CGTATTGTTG AACACCCTTC AGACCTAATT GTGTCAAAAG GAGAACCTGC AACTCTGAAC
    TGTAAAGCTG AAGGCCGCCC CACGCCCACC ATTGAATGGT ACAAAGGTGG GGAGAGAGTG
    GAGACAGACA AAGATGACCC TCGCTCACAC CGAATGTTGC TGCCAAGTGG ATCTTTATTT
    TTCTTACGTA TAGTACATGG ACGAAAAAGT AGGCCTGATG AAGGGGTCTA CGTCTGTGTG
    GCACGGAATT ACCTCGGAGA GGCTGTGAGC CATAATGCAT CGCTGGAGGT GGCTATACTA
    CGGGATGACT TCAGACAAAA TCCTTCTGAT GTCATGGTGG CAGTGGGGGA ACCTGCTGTG
    ATGGAATGCC AACCCCCACG AGGCCATCCT GAACCCACCA TTTCGTGGAA GAAAGATGGC
    TCTCCACTGG ATGATAAAGA TGAAAGAATA ACTATACGAG GTGGAAAGCT CATGATTACT
    TACACCCGCA AAAGTGATGC TGGCAAATAT GTTTGTGTTG GTACCAACAT GGTTGGGGAA
    CGTGAGAGTG AAGTAGCAGA GCTAACTGTC TTGGAGAGAC CATCATTTGT GAAGAGACCT
    AGTAACTTGG CTGTAACTGT GGATGACAGT GCAGAATTTA AATGTGAGGC TCGAGGTGAC
    CCTGTGCCTA CGGTACGATG GAGGAAAGAC GATGGAGAAC TGCCCAAATC CAGATACGAA
    ATCAGAGATG ATCATACTTT GAAGATTCGG AAAGTGATGG CTGGTGACAT GGGGTCATAT
    ACTTGTGTTG CAGAAAATAT GGTTGGAAAA GCTGAAGCAT CAGCTACTCT GACTGTCCAA
    GTTGGGTCTG AACCTCCACA TTTTGTTGTG AAACCTCGTG ACCAGGTTGT TGCCTTGGGG
    CGAACTGTAA CATTTCAGTG TGAAGCAACC GGAAATCCTC AACCAGCTAT TTTTTGGAGA
    AGAGAGGGGA GTCAGAATCT GCTTTTCTCA TATCAGCCGC CACAGTCAAC TAGCCGCTTT
    TCCGTCTCCC AGACTGGTGA TCTCACTATT ACTAATGTCC AGCGGTCTGA TGTTGGTTAT
    TATATCTGCC AGACTTTAAA TGTTGCCGGA AGTATCATCA CAAAAGCATA TTTGGAAGTT
    ACTGATGTGA TTGCAGACCG GCCTCCCCCA GTCATTCGAC AAGGTCCTGT GAATCAGACT
    GTAGCAGTGG ATGGTACTTT AGTGCTCAGC TGTGTGGCCA CAGGCAGTCC AATGCCCACG
    ATTCTCTGGA GAAAGGATGG AGTCCTTGTT TCAACCCAAG ATTCTAGAAT CAAACAGCTG
    GAGACGGGAG TGCTGCAGAT CCGATACGCT AAGCTGGGTG ACACAGGTCG GTACACCTGC
    ATTGCATCAA CCCCCAGTGG TGAAGCAACA TGGAGTGCTT ACATTGAAGT CCAAGAATTT
    GGAGTTCCAG TTCAGCCTCC ACGACCCACC GACCCAAATT TAATCCCTAG TGCCCCCTCA
    AAACCTGAAG TTACAGATGT CAGCAGAAAT ACAGTAACGT TGTCATGGCA ACCAAATTTG
    AATTCAGGTG CAACTCCAAC ATCTTATATT ATAGAAGCCT TCAGCCATGC ATCTGGTAGC
    AGCTGGCAGA CCGTAGCAGA GAATGTGAAA ACAGAAACAT TTGCCATTAA AGGACTCAAA
    CCTAATGCAA TTTACCTTTT CCTTGTGAGG GCAGCAAATG CCTATGGAAT TAGTGATCCA
    AGCCAGATAT CAGATCCAGT GAAAACACAA GATGTTCCCC CAACAAGTCA GGGAGTAGAT
    CACAAGCAGG TCCAGAGAGA ATTGGGAAAC GTCGTACTGC ACCTTCACAA CCCCACCATC
    CTCTCATCCT CTTCCATCGA AGTGCACTGG ACAGTGGACC AACAATCTCA GTATATTCAA
    GGATATAAAA TTCTTTATCG GCCATCTGGA GGCAACCATG GTGAATCGGA GTGGTTAGTT
    TTTGAAGTGA GGACTCCAAC CAAAAATAGC GTGGTTATCC CTGATCTCAA AAAGGGAGTC
    AACTATGAAA TTAAGGCTCG TCCTTTTTTT AATGAATTTC AAGGAGCAGA TAGTGAAATT
    AAATTTGCCA AAACCTTAGA AGAAGCACCC AGTGCCCCAC CCCAAAGTGT AACCGTATCA
    AAGAATGATG GAAATGGAAC TGCAATTCTT GTTAGTTGGC AGCCACCTCC AGAAGACACT
    CAAAATGGAA TGGTCCAAGA GTATAAGGTT TGGTGTCTGG GCAATGAAAC TCGTTATCAC
    ATAAACAAGA CGGTGGATGG TTCCACCTTT TCTGTGGTCA TTCCTTCTCT GGTTCCTGGG
    ATCCGATACA GCGTAGAAGT AGCAGCCAGC ACGGGAGCTG GAGCTGGGGT GAAGAGCGAG
    CCACAGTTCA TCCAGTTAGA TTCTCATGGA AACCCCGTGT CACCTGAAGA CCAAGTCAGC
    CTTGCTCAGC AGATCTCAGA TGTTGTGAAG CAGCCAGCGT TCATTGCGGG AATCGGGGCC
    GCCTGCTGGA TCATCCTCAT GGTCTTTAGC ATCTGGCTCT ATCGACACCG GAAGAAGAGA
    AATGGACTGA CTAGTACTTA TGCGGGTATC AGAAAAGTCC CGTCTTTTAC CTTCACACCA
    ACAGTAACTT ATCAGAGAGG AGGTGAAGCT GTGAGCAGTG GAGGGAGGCC AGGACTTCTG
    AACATCAGCG AACCTGCCAC ACAGCCCTGG CTGGCAGACA CGTGGCCCAA TACTGGCAAC
    AATCACAATG ACTGCTCTAT CAATTGCTGT ACGGCAGGCA ATGGTAACAG TGATAACAAC
    CTGACCACCT ACAGTCGCCC AGGGCAGCCC ACTCCTTATG CCACCACCCA GCTCATCCAG
    TCAAACCTCA GCAACAACAT GAACAATGGC AGCGGGGACT CCAGCGAGAA GCACTGGAAA
    CCTCCCGGCC AGCAGAAACA AGAAGTGCCA CCAGTTCAGT ATAACATCAT GGAGCAAAAC
    AAACTGAACA AAGATTATCG AGCAAATGAC ACAGTTCCTC CAACTATCCC ATACAACCAA
    TCATATGACC AGAATACAGG AGGATCCTAC AACAGTTCAG ACCGGGGCAG TAGTACATCT
    GGGAGTCAAG GGCACAAGAA AGGGGCACGG ACACCCAAAG TACCAAAACA AGGTGGCATG
    AACTGGGCAG ACCTGCTTCC TCCTCCCCCA GCGCATCCTC CTCCTCATAG CAATAGTGAA
    GAGTACAATA TTTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGTCC CGTGCCACCA
    GCAAGGATGT ATTTGCAACA GAATGAACTA GAAGAGGAGG AAGATGAGCG AGGCCCCACT
    CCCCCTGTGC GGGGAGCAGC TTCTTCTCCA GCTGCTGTGT CTTATAGCCA TCAGTCCACT
    GCTACTCTGA CCCCCTCCCC ACAAGAAGAA CTCCAGCCCA TGTTGCAGGA CTGCCCGGAG
    GAGATTGGCC ACATGCAGCA CCAACCCGAT AGGAGACGGC AGCCTGTGAG TCCTCCTCCA
    CCACCAAGAC CGATTTCCCC ACCACATACC TATGGCTACA TTTCAGGACC TCTTGTCTCA
    GATATGGATA CAGATGCACC AGAAGAGGAA GAAGATGAAG CAGACATGGA AGTGGCCAAG
    ATGCAGACCA GACGGCTTTT GTTACGTGGC CTTGAGCAGA CCCCTGCCTC CAGTGTGGGG
    GACCTTGAAA GCTCTGTCAC TGGGTCCATG ATCAATGGCT GGGGCTCAGC CTCAGAGGAG
    GACAACATTT CCAGTGGACG CTCCAGTGTG AGTTCTTCTG ATGGCTCCTT CTTCACTGAT
    GCTGATTTTG CCCAGGCAGT GGCAGCAGCT GCAGAATATG CTGGTCTTAA AGTGGCACGT
    CGTCAGATGC AGGATGCTGC TGGTCGCCGA CATTTTCATG CATCCCAGTG CCCTCGACCC
    ACAAGTCCTG TCTCTACAGA CAGCAACATG AGTGCTGCTG TAATGCAGAA GGCCAGACCA
    GCCAAGAAGC AAAAACACCA GCCAGGACAT CTGCGCAGAG AAGTCTACAC AGATGATCTA
    CCACCTCCTC CGGTGCCACC ACCTGCTATA AAATCACCCA CTGTCCAATC CAAGGCACAG
    CTGGAGGTGC GACCTGTTAT GGTGCCAAAA CTCCCTTCTA TAGAAGCAAG AACAGACCGA
    TCATCAGATA GAAAAGTAGG CAGTTACAAG GGGAGAGAAG TACTGGATGG AAGACAGGTT
    GCTGATATGA GAACCAATCC AGGTGATACC AGAGAAGCAC AGGAACAGCA AAATGATGGA
    AAAGGACGAG TAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGAC TCACCTCATC
    CAAGAGGATA TCCTACCTTA CTGTAGACCT ACTTTTCCGA CATCAAATAA TCCCAGAGAT
    CCTAGTTCTT CAAGCTCAAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA
    AATGTAGGTC GAAGAAATAT CGCAGAAATG CAAGTTCTTG GAGGATATGA AAGAGGAGAA
    GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268821.1
    CDS16..4911
    Translation

    Target ORF information:

    RefSeq Version XM_022413113.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413113.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCTGAGAC CTCCGTGCAG CTCAGTATGC TGCAGGAGGG GCAAGGAGGA AGTGTGCTGT 
    GTGTTGTCCC TGTGTGTTTT TCTGTCACAT GTGGATTTAC TTGTGTCTGG ACATGCTGTG
    ATCGAAGTGG CAACTACCCT TACTACTGTG ACCCCATCAG CAGCTCTGGT GTTGTTTCCG
    ACAGACCCTG AAGACCTAGA GAGGGGGAAC GACAACGGGA CGCCAATCCC CACCTCTGAT
    AATGATGACA ATTCGCTGGG CTACACAGGC TCCCGTCTCC GTCAGGAAGA TTTTCCACCT
    CGTATTGTTG AACACCCTTC AGACCTAATT GTGTCAAAAG GAGAACCTGC AACTCTGAAC
    TGTAAAGCTG AAGGCCGCCC CACGCCCACC ATTGAATGGT ACAAAGGTGG GGAGAGAGTG
    GAGACAGACA AAGATGACCC TCGCTCACAC CGAATGTTGC TGCCAAGTGG ATCTTTATTT
    TTCTTACGTA TAGTACATGG ACGAAAAAGT AGGCCTGATG AAGGGGTCTA CGTCTGTGTG
    GCACGGAATT ACCTCGGAGA GGCTGTGAGC CATAATGCAT CGCTGGAGGT GGCTATACTA
    CGGGATGACT TCAGACAAAA TCCTTCTGAT GTCATGGTGG CAGTGGGGGA ACCTGCTGTG
    ATGGAATGCC AACCCCCACG AGGCCATCCT GAACCCACCA TTTCGTGGAA GAAAGATGGC
    TCTCCACTGG ATGATAAAGA TGAAAGAATA ACTATACGAG GTGGAAAGCT CATGATTACT
    TACACCCGCA AAAGTGATGC TGGCAAATAT GTTTGTGTTG GTACCAACAT GGTTGGGGAA
    CGTGAGAGTG AAGTAGCAGA GCTAACTGTC TTGGAGAGAC CATCATTTGT GAAGAGACCT
    AGTAACTTGG CTGTAACTGT GGATGACAGT GCAGAATTTA AATGTGAGGC TCGAGGTGAC
    CCTGTGCCTA CGGTACGATG GAGGAAAGAC GATGGAGAAC TGCCCAAATC CAGATACGAA
    ATCAGAGATG ATCATACTTT GAAGATTCGG AAAGTGATGG CTGGTGACAT GGGGTCATAT
    ACTTGTGTTG CAGAAAATAT GGTTGGAAAA GCTGAAGCAT CAGCTACTCT GACTGTCCAA
    GTTGGGTCTG AACCTCCACA TTTTGTTGTG AAACCTCGTG ACCAGGTTGT TGCCTTGGGG
    CGAACTGTAA CATTTCAGTG TGAAGCAACC GGAAATCCTC AACCAGCTAT TTTTTGGAGA
    AGAGAGGGGA GTCAGAATCT GCTTTTCTCA TATCAGCCGC CACAGTCAAC TAGCCGCTTT
    TCCGTCTCCC AGACTGGTGA TCTCACTATT ACTAATGTCC AGCGGTCTGA TGTTGGTTAT
    TATATCTGCC AGACTTTAAA TGTTGCCGGA AGTATCATCA CAAAAGCATA TTTGGAAGTT
    ACTGATGTGA TTGCAGACCG GCCTCCCCCA GTCATTCGAC AAGGTCCTGT GAATCAGACT
    GTAGCAGTGG ATGGTACTTT AGTGCTCAGC TGTGTGGCCA CAGGCAGTCC AATGCCCACG
    ATTCTCTGGA GAAAGGATGG AGTCCTTGTT TCAACCCAAG ATTCTAGAAT CAAACAGCTG
    GAGACGGGAG TGCTGCAGAT CCGATACGCT AAGCTGGGTG ACACAGGTCG GTACACCTGC
    ATTGCATCAA CCCCCAGTGG TGAAGCAACA TGGAGTGCTT ACATTGAAGT CCAAGAATTT
    GGAGTTCCAG TTCAGCCTCC ACGACCCACC GACCCAAATT TAATCCCTAG TGCCCCCTCA
    AAACCTGAAG TTACAGATGT CAGCAGAAAT ACAGTAACGT TGTCATGGCA ACCAAATTTG
    AATTCAGGTG CAACTCCAAC ATCTTATATT ATAGAAGCCT TCAGCCATGC ATCTGGTAGC
    AGCTGGCAGA CCGTAGCAGA GAATGTGAAA ACAGAAACAT TTGCCATTAA AGGACTCAAA
    CCTAATGCAA TTTACCTTTT CCTTGTGAGG GCAGCAAATG CCTATGGAAT TAGTGATCCA
    AGCCAGATAT CAGATCCAGT GAAAACACAA GATGTTCCCC CAACAAGTCA GGGAGTAGAT
    CACAAGCAGG TCCAGAGAGA ATTGGGAAAC GTCGTACTGC ACCTTCACAA CCCCACCATC
    CTCTCATCCT CTTCCATCGA AGTGCACTGG ACAGTGGACC AACAATCTCA GTATATTCAA
    GGATATAAAA TTCTTTATCG GCCATCTGGA GGCAACCATG GTGAATCGGA GTGGTTAGTT
    TTTGAAGTGA GGACTCCAAC CAAAAATAGC GTGGTTATCC CTGATCTCAA AAAGGGAGTC
    AACTATGAAA TTAAGGCTCG TCCTTTTTTT AATGAATTTC AAGGAGCAGA TAGTGAAATT
    AAATTTGCCA AAACCTTAGA AGAAGCACCC AGTGCCCCAC CCCAAAGTGT AACCGTATCA
    AAGAATGATG GAAATGGAAC TGCAATTCTT GTTAGTTGGC AGCCACCTCC AGAAGACACT
    CAAAATGGAA TGGTCCAAGA GTATAAGGTT TGGTGTCTGG GCAATGAAAC TCGTTATCAC
    ATAAACAAGA CGGTGGATGG TTCCACCTTT TCTGTGGTCA TTCCTTCTCT GGTTCCTGGG
    ATCCGATACA GCGTAGAAGT AGCAGCCAGC ACGGGAGCTG GAGCTGGGGT GAAGAGCGAG
    CCACAGTTCA TCCAGTTAGA TTCTCATGGA AACCCCGTGT CACCTGAAGA CCAAGTCAGC
    CTTGCTCAGC AGATCTCAGA TGTTGTGAAG CAGCCAGCGT TCATTGCGGG AATCGGGGCC
    GCCTGCTGGA TCATCCTCAT GGTCTTTAGC ATCTGGCTCT ATCGACACCG GAAGAAGAGA
    AATGGACTGA CTAGTACTTA TGCGGGTATC AGAAAAGTCC CGTCTTTTAC CTTCACACCA
    ACAGTAACTT ATCAGAGAGG AGGTGAAGCT GTGAGCAGTG GAGGGAGGCC AGGACTTCTG
    AACATCAGCG AACCTGCCAC ACAGCCCTGG CTGGCAGACA CGTGGCCCAA TACTGGCAAC
    AATCACAATG ACTGCTCTAT CAATTGCTGT ACGGCAGGCA ATGGTAACAG TGATAACAAC
    CTGACCACCT ACAGTCGCCC AGGGCAGCCC ACTCCTTATG CCACCACCCA GCTCATCCAG
    TCAAACCTCA GCAACAACAT GAACAATGGC AGCGGGGACT CCAGCGAGAA GCACTGGAAA
    CCTCCCGGCC AGCAGAAACA AGAAGTGCCA CCAGTTCAGT ATAACATCAT GGAGCAAAAC
    AAACTGAACA AAGATTATCG AGCAAATGAC ACAGTTCCTC CAACTATCCC ATACAACCAA
    TCATATGACC AGAATACAGG AGGATCCTAC AACAGTTCAG ACCGGGGCAG TAGTACATCT
    GGGAGTCAAG GGCACAAGAA AGGGGCACGG ACACCCAAAG TACCAAAACA AGGTGGCATG
    AACTGGGCAG ACCTGCTTCC TCCTCCCCCA GCGCATCCTC CTCCTCATAG CAATAGTGAA
    GAGTACAATA TTTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGTCC CGTGCCACCA
    GCAAGGATGT ATTTGCAACA GAATGAACTA GAAGAGGAGG AAGATGAGCG AGGCCCCACT
    CCCCCTGTGC GGGGAGCAGC TTCTTCTCCA GCTGCTGTGT CTTATAGCCA TCAGTCCACT
    GCTACTCTGA CCCCCTCCCC ACAAGAAGAA CTCCAGCCCA TGTTGCAGGA CTGCCCGGAG
    GAGATTGGCC ACATGCAGCA CCAACCCGAT AGGAGACGGC AGCCTGTGAG TCCTCCTCCA
    CCACCAAGAC CGATTTCCCC ACCACATACC TATGGCTACA TTTCAGGACC TCTTGTCTCA
    GATATGGATA CAGATGCACC AGAAGAGGAA GAAGATGAAG CAGACATGGA AGTGGCCAAG
    ATGCAGACCA GACGGCTTTT GTTACGTGGC CTTGAGCAGA CCCCTGCCTC CAGTGTGGGG
    GACCTTGAAA GCTCTGTCAC TGGGTCCATG ATCAATGGCT GGGGCTCAGC CTCAGAGGAG
    GACAACATTT CCAGTGGACG CTCCAGTGTG AGTTCTTCTG ATGGCTCCTT CTTCACTGAT
    GCTGATTTTG CCCAGGCAGT GGCAGCAGCT GCAGAATATG CTGGTCTTAA AGTGGCACGT
    CGTCAGATGC AGGATGCTGC TGGTCGCCGA CATTTTCATG CATCCCAGTG CCCTCGACCC
    ACAAGTCCTG TCTCTACAGA CAGCAACATG AGTGCTGCTG TAATGCAGAA GGCCAGACCA
    GCCAAGAAGC AAAAACACCA GCCAGGACAT CTGCGCAGAG AAGTCTACAC AGATGATCTA
    CCACCTCCTC CGGTGCCACC ACCTGCTATA AAATCACCCA CTGTCCAATC CAAGGCACAG
    CTGGAGGTGC GACCTGTTAT GGTGCCAAAA CTCCCTTCTA TAGAAGCAAG AACAGACCGA
    TCATCAGATA GAAAAGTAGG CAGTTACAAG GGGAGAGAAG TACTGGATGG AAGACAGGTT
    GCTGATATGA GAACCAATCC AGGTGATACC AGAGAAGCAC AGGAACAGCA AAATGATGGA
    AAAGGACGAG TAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGAC TCACCTCATC
    CAAGAGGATA TCCTACCTTA CTGTAGACCT ACTTTTCCGA CATCAAATAA TCCCAGAGAT
    CCTAGTTCTT CAAGCTCAAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA
    AATGTAGGTC GAAGAAATAT CGCAGAAATG CAAGTTCTTG GAGGATATGA AAGAGGAGAA
    GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe283889
    Clone ID Related Accession (Same CDS sequence) XM_022413114.1
    Accession Version XM_022413114.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4869bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product roundabout homolog 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCTGAGAC CTCCGTGCAG CTCAGTATGC TGCAGGAGGG GCAAGGAGGA AGTGTGCTGT 
    GTGTTGTCCC TGTGTGTTTT TCTGTCACAT GTGGATTTAC TTGTGTCTGG ACATGCTGTG
    ATCGAAGTGG CAACTACCCT TACTACTGTG ACCCCATCAG CAGCTCTGGT GTTGTTTCCG
    ACAGACCCTG AAGACCTAGA GAGGGGGAAC GACAACGGGA CGCCAATCCC CACCTCTGAT
    AATGATGACA ATTCGCTGGG CTACACAGGC TCCCGTCTCC GTCAGGAAGA TTTTCCACCT
    CGTATTGTTG AACACCCTTC AGACCTAATT GTGTCAAAAG GAGAACCTGC AACTCTGAAC
    TGTAAAGCTG AAGGCCGCCC CACGCCCACC ATTGAATGGT ACAAAGGTGG GGAGAGAGTG
    GAGACAGACA AAGATGACCC TCGCTCACAC CGAATGTTGC TGCCAAGTGG ATCTTTATTT
    TTCTTACGTA TAGTACATGG ACGAAAAAGT AGGCCTGATG AAGGGGTCTA CGTCTGTGTG
    GCACGGAATT ACCTCGGAGA GGCTGTGAGC CATAATGCAT CGCTGGAGGT GGCTATACTA
    CGGGATGACT TCAGACAAAA TCCTTCTGAT GTCATGGTGG CAGTGGGGGA ACCTGCTGTG
    ATGGAATGCC AACCCCCACG AGGCCATCCT GAACCCACCA TTTCGTGGAA GAAAGATGGC
    TCTCCACTGG ATGATAAAGA TGAAAGAATA ACTATACGAG GTGGAAAGCT CATGATTACT
    TACACCCGCA AAAGTGATGC TGGCAAATAT GTTTGTGTTG GTACCAACAT GGTTGGGGAA
    CGTGAGAGTG AAGTAGCAGA GCTAACTGTC TTGGAGAGAC CATCATTTGT GAAGAGACCT
    AGTAACTTGG CTGTAACTGT GGATGACAGT GCAGAATTTA AATGTGAGGC TCGAGGTGAC
    CCTGTGCCTA CGGTACGATG GAGGAAAGAC GATGGAGAAC TGCCCAAATC CAGATACGAA
    ATCAGAGATG ATCATACTTT GAAGATTCGG AAAGTGATGG CTGGTGACAT GGGGTCATAT
    ACTTGTGTTG CAGAAAATAT GGTTGGAAAA GCTGAAGCAT CAGCTACTCT GACTGTCCAA
    GTTGGGTCTG AACCTCCACA TTTTGTTGTG AAACCTCGTG ACCAGGTTGT TGCCTTGGGG
    CGAACTGTAA CATTTCAGTG TGAAGCAACC GGAAATCCTC AACCAGCTAT TTTTTGGAGA
    AGAGAGGGGA GTCAGAATCT GCTTTTCTCA TATCAGCCGC CACAGTCAAC TAGCCGCTTT
    TCCGTCTCCC AGACTGGTGA TCTCACTATT ACTAATGTCC AGCGGTCTGA TGTTGGTTAT
    TATATCTGCC AGACTTTAAA TGTTGCCGGA AGTATCATCA CAAAAGCATA TTTGGAAGTT
    ACTGATGTGA TTGCAGACCG GCCTCCCCCA GTCATTCGAC AAGGTCCTGT GAATCAGACT
    GTAGCAGTGG ATGGTACTTT AGTGCTCAGC TGTGTGGCCA CAGGCAGTCC AATGCCCACG
    ATTCTCTGGA GAAAGGATGG AGTCCTTGTT TCAACCCAAG ATTCTAGAAT CAAACAGCTG
    GAGACGGGAG TGCTGCAGAT CCGATACGCT AAGCTGGGTG ACACAGGTCG GTACACCTGC
    ATTGCATCAA CCCCCAGTGG TGAAGCAACA TGGAGTGCTT ACATTGAAGT CCAAGAATTT
    GGAGTTCCAG TTCAGCCTCC ACGACCCACC GACCCAAATT TAATCCCTAG TGCCCCCTCA
    AAACCTGAAG TTACAGATGT CAGCAGAAAT ACAGTAACGT TGTCATGGCA ACCAAATTTG
    AATTCAGGTG CAACTCCAAC ATCTTATATT ATAGAAGCCT TCAGCCATGC ATCTGGTAGC
    AGCTGGCAGA CCGTAGCAGA GAATGTGAAA ACAGAAACAT TTGCCATTAA AGGACTCAAA
    CCTAATGCAA TTTACCTTTT CCTTGTGAGG GCAGCAAATG CCTATGGAAT TAGTGATCCA
    AGCCAGATAT CAGATCCAGT GAAAACACAA GATGTTCCCC CAACAAGTCA GGGAGTAGAT
    CACAAGCAGG TCCAGAGAGA ATTGGGAAAC GTCGTACTGC ACCTTCACAA CCCCACCATC
    CTCTCATCCT CTTCCATCGA AGTGCACTGG ACAGTGGACC AACAATCTCA GTATATTCAA
    GGATATAAAA TTCTTTATCG GCCATCTGGA GGCAACCATG GTGAATCGGA GTGGTTAGTT
    TTTGAAGTGA GGACTCCAAC CAAAAATAGC GTGGTTATCC CTGATCTCAA AAAGGGAGTC
    AACTATGAAA TTAAGGCTCG TCCTTTTTTT AATGAATTTC AAGGAGCAGA TAGTGAAATT
    AAATTTGCCA AAACCTTAGA AGAAGCACCC AGTGCCCCAC CCCAAAGTGT AACCGTATCA
    AAGAATGATG GAAATGGAAC TGCAATTCTT GTTAGTTGGC AGCCACCTCC AGAAGACACT
    CAAAATGGAA TGGTCCAAGA GTATAAGGTT TGGTGTCTGG GCAATGAAAC TCGTTATCAC
    ATAAACAAGA CGGTGGATGG TTCCACCTTT TCTGTGGTCA TTCCTTCTCT GGTTCCTGGG
    ATCCGATACA GCGTAGAAGT AGCAGCCAGC ACGGGAGCTG GAGCTGGGGT GAAGAGCGAG
    CCACAGTTCA TCCAGTTAGA TTCTCATGGA AACCCCGTGT CACCTGAAGA CCAAGTCAGC
    CTTGCTCAGC AGATCTCAGA TGTTGTGAAG CAGCCAGCGT TCATTGCGGG AATCGGGGCC
    GCCTGCTGGA TCATCCTCAT GGTCTTTAGC ATCTGGCTCT ATCGACACCG GAAGAAGAGA
    AATGGACTGA CTAGTACTTA TGCGGGTATC AGAAAAGTAA CTTATCAGAG AGGAGGTGAA
    GCTGTGAGCA GTGGAGGGAG GCCAGGACTT CTGAACATCA GCGAACCTGC CACACAGCCC
    TGGCTGGCAG ACACGTGGCC CAATACTGGC AACAATCACA ATGACTGCTC TATCAATTGC
    TGTACGGCAG GCAATGGTAA CAGTGATAAC AACCTGACCA CCTACAGTCG CCCAGGGCAG
    CCCACTCCTT ATGCCACCAC CCAGCTCATC CAGTCAAACC TCAGCAACAA CATGAACAAT
    GGCAGCGGGG ACTCCAGCGA GAAGCACTGG AAACCTCCCG GCCAGCAGAA ACAAGAAGTG
    CCACCAGTTC AGTATAACAT CATGGAGCAA AACAAACTGA ACAAAGATTA TCGAGCAAAT
    GACACAGTTC CTCCAACTAT CCCATACAAC CAATCATATG ACCAGAATAC AGGAGGATCC
    TACAACAGTT CAGACCGGGG CAGTAGTACA TCTGGGAGTC AAGGGCACAA GAAAGGGGCA
    CGGACACCCA AAGTACCAAA ACAAGGTGGC ATGAACTGGG CAGACCTGCT TCCTCCTCCC
    CCAGCGCATC CTCCTCCTCA TAGCAATAGT GAAGAGTACA ATATTTCTGT AGATGAAAGC
    TATGACCAAG AAATGCCATG TCCCGTGCCA CCAGCAAGGA TGTATTTGCA ACAGAATGAA
    CTAGAAGAGG AGGAAGATGA GCGAGGCCCC ACTCCCCCTG TGCGGGGAGC AGCTTCTTCT
    CCAGCTGCTG TGTCTTATAG CCATCAGTCC ACTGCTACTC TGACCCCCTC CCCACAAGAA
    GAACTCCAGC CCATGTTGCA GGACTGCCCG GAGGAGATTG GCCACATGCA GCACCAACCC
    GATAGGAGAC GGCAGCCTGT GAGTCCTCCT CCACCACCAA GACCGATTTC CCCACCACAT
    ACCTATGGCT ACATTTCAGG ACCTCTTGTC TCAGATATGG ATACAGATGC ACCAGAAGAG
    GAAGAAGATG AAGCAGACAT GGAAGTGGCC AAGATGCAGA CCAGACGGCT TTTGTTACGT
    GGCCTTGAGC AGACCCCTGC CTCCAGTGTG GGGGACCTTG AAAGCTCTGT CACTGGGTCC
    ATGATCAATG GCTGGGGCTC AGCCTCAGAG GAGGACAACA TTTCCAGTGG ACGCTCCAGT
    GTGAGTTCTT CTGATGGCTC CTTCTTCACT GATGCTGATT TTGCCCAGGC AGTGGCAGCA
    GCTGCAGAAT ATGCTGGTCT TAAAGTGGCA CGTCGTCAGA TGCAGGATGC TGCTGGTCGC
    CGACATTTTC ATGCATCCCA GTGCCCTCGA CCCACAAGTC CTGTCTCTAC AGACAGCAAC
    ATGAGTGCTG CTGTAATGCA GAAGGCCAGA CCAGCCAAGA AGCAAAAACA CCAGCCAGGA
    CATCTGCGCA GAGAAGTCTA CACAGATGAT CTACCACCTC CTCCGGTGCC ACCACCTGCT
    ATAAAATCAC CCACTGTCCA ATCCAAGGCA CAGCTGGAGG TGCGACCTGT TATGGTGCCA
    AAACTCCCTT CTATAGAAGC AAGAACAGAC CGATCATCAG ATAGAAAAGT AGGCAGTTAC
    AAGGGGAGAG AAGTACTGGA TGGAAGACAG GTTGCTGATA TGAGAACCAA TCCAGGTGAT
    ACCAGAGAAG CACAGGAACA GCAAAATGAT GGAAAAGGAC GAGTAAACAA GGCAGCAAAA
    CGAGACCTTC CACCAGCAAA GACTCACCTC ATCCAAGAGG ATATCCTACC TTACTGTAGA
    CCTACTTTTC CGACATCAAA TAATCCCAGA GATCCTAGTT CTTCAAGCTC AATGTCATCA
    AGAGGATCAG GAAGCAGACA AAGAGAACAA GCAAATGTAG GTCGAAGAAA TATCGCAGAA
    ATGCAAGTTC TTGGAGGATA TGAAAGAGGA GAAGATAATA ATGAAGAATT AGAGGAAACT
    GAAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268822.1
    CDS16..4884
    Translation

    Target ORF information:

    RefSeq Version XM_022413114.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413114.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCTGAGAC CTCCGTGCAG CTCAGTATGC TGCAGGAGGG GCAAGGAGGA AGTGTGCTGT 
    GTGTTGTCCC TGTGTGTTTT TCTGTCACAT GTGGATTTAC TTGTGTCTGG ACATGCTGTG
    ATCGAAGTGG CAACTACCCT TACTACTGTG ACCCCATCAG CAGCTCTGGT GTTGTTTCCG
    ACAGACCCTG AAGACCTAGA GAGGGGGAAC GACAACGGGA CGCCAATCCC CACCTCTGAT
    AATGATGACA ATTCGCTGGG CTACACAGGC TCCCGTCTCC GTCAGGAAGA TTTTCCACCT
    CGTATTGTTG AACACCCTTC AGACCTAATT GTGTCAAAAG GAGAACCTGC AACTCTGAAC
    TGTAAAGCTG AAGGCCGCCC CACGCCCACC ATTGAATGGT ACAAAGGTGG GGAGAGAGTG
    GAGACAGACA AAGATGACCC TCGCTCACAC CGAATGTTGC TGCCAAGTGG ATCTTTATTT
    TTCTTACGTA TAGTACATGG ACGAAAAAGT AGGCCTGATG AAGGGGTCTA CGTCTGTGTG
    GCACGGAATT ACCTCGGAGA GGCTGTGAGC CATAATGCAT CGCTGGAGGT GGCTATACTA
    CGGGATGACT TCAGACAAAA TCCTTCTGAT GTCATGGTGG CAGTGGGGGA ACCTGCTGTG
    ATGGAATGCC AACCCCCACG AGGCCATCCT GAACCCACCA TTTCGTGGAA GAAAGATGGC
    TCTCCACTGG ATGATAAAGA TGAAAGAATA ACTATACGAG GTGGAAAGCT CATGATTACT
    TACACCCGCA AAAGTGATGC TGGCAAATAT GTTTGTGTTG GTACCAACAT GGTTGGGGAA
    CGTGAGAGTG AAGTAGCAGA GCTAACTGTC TTGGAGAGAC CATCATTTGT GAAGAGACCT
    AGTAACTTGG CTGTAACTGT GGATGACAGT GCAGAATTTA AATGTGAGGC TCGAGGTGAC
    CCTGTGCCTA CGGTACGATG GAGGAAAGAC GATGGAGAAC TGCCCAAATC CAGATACGAA
    ATCAGAGATG ATCATACTTT GAAGATTCGG AAAGTGATGG CTGGTGACAT GGGGTCATAT
    ACTTGTGTTG CAGAAAATAT GGTTGGAAAA GCTGAAGCAT CAGCTACTCT GACTGTCCAA
    GTTGGGTCTG AACCTCCACA TTTTGTTGTG AAACCTCGTG ACCAGGTTGT TGCCTTGGGG
    CGAACTGTAA CATTTCAGTG TGAAGCAACC GGAAATCCTC AACCAGCTAT TTTTTGGAGA
    AGAGAGGGGA GTCAGAATCT GCTTTTCTCA TATCAGCCGC CACAGTCAAC TAGCCGCTTT
    TCCGTCTCCC AGACTGGTGA TCTCACTATT ACTAATGTCC AGCGGTCTGA TGTTGGTTAT
    TATATCTGCC AGACTTTAAA TGTTGCCGGA AGTATCATCA CAAAAGCATA TTTGGAAGTT
    ACTGATGTGA TTGCAGACCG GCCTCCCCCA GTCATTCGAC AAGGTCCTGT GAATCAGACT
    GTAGCAGTGG ATGGTACTTT AGTGCTCAGC TGTGTGGCCA CAGGCAGTCC AATGCCCACG
    ATTCTCTGGA GAAAGGATGG AGTCCTTGTT TCAACCCAAG ATTCTAGAAT CAAACAGCTG
    GAGACGGGAG TGCTGCAGAT CCGATACGCT AAGCTGGGTG ACACAGGTCG GTACACCTGC
    ATTGCATCAA CCCCCAGTGG TGAAGCAACA TGGAGTGCTT ACATTGAAGT CCAAGAATTT
    GGAGTTCCAG TTCAGCCTCC ACGACCCACC GACCCAAATT TAATCCCTAG TGCCCCCTCA
    AAACCTGAAG TTACAGATGT CAGCAGAAAT ACAGTAACGT TGTCATGGCA ACCAAATTTG
    AATTCAGGTG CAACTCCAAC ATCTTATATT ATAGAAGCCT TCAGCCATGC ATCTGGTAGC
    AGCTGGCAGA CCGTAGCAGA GAATGTGAAA ACAGAAACAT TTGCCATTAA AGGACTCAAA
    CCTAATGCAA TTTACCTTTT CCTTGTGAGG GCAGCAAATG CCTATGGAAT TAGTGATCCA
    AGCCAGATAT CAGATCCAGT GAAAACACAA GATGTTCCCC CAACAAGTCA GGGAGTAGAT
    CACAAGCAGG TCCAGAGAGA ATTGGGAAAC GTCGTACTGC ACCTTCACAA CCCCACCATC
    CTCTCATCCT CTTCCATCGA AGTGCACTGG ACAGTGGACC AACAATCTCA GTATATTCAA
    GGATATAAAA TTCTTTATCG GCCATCTGGA GGCAACCATG GTGAATCGGA GTGGTTAGTT
    TTTGAAGTGA GGACTCCAAC CAAAAATAGC GTGGTTATCC CTGATCTCAA AAAGGGAGTC
    AACTATGAAA TTAAGGCTCG TCCTTTTTTT AATGAATTTC AAGGAGCAGA TAGTGAAATT
    AAATTTGCCA AAACCTTAGA AGAAGCACCC AGTGCCCCAC CCCAAAGTGT AACCGTATCA
    AAGAATGATG GAAATGGAAC TGCAATTCTT GTTAGTTGGC AGCCACCTCC AGAAGACACT
    CAAAATGGAA TGGTCCAAGA GTATAAGGTT TGGTGTCTGG GCAATGAAAC TCGTTATCAC
    ATAAACAAGA CGGTGGATGG TTCCACCTTT TCTGTGGTCA TTCCTTCTCT GGTTCCTGGG
    ATCCGATACA GCGTAGAAGT AGCAGCCAGC ACGGGAGCTG GAGCTGGGGT GAAGAGCGAG
    CCACAGTTCA TCCAGTTAGA TTCTCATGGA AACCCCGTGT CACCTGAAGA CCAAGTCAGC
    CTTGCTCAGC AGATCTCAGA TGTTGTGAAG CAGCCAGCGT TCATTGCGGG AATCGGGGCC
    GCCTGCTGGA TCATCCTCAT GGTCTTTAGC ATCTGGCTCT ATCGACACCG GAAGAAGAGA
    AATGGACTGA CTAGTACTTA TGCGGGTATC AGAAAAGTAA CTTATCAGAG AGGAGGTGAA
    GCTGTGAGCA GTGGAGGGAG GCCAGGACTT CTGAACATCA GCGAACCTGC CACACAGCCC
    TGGCTGGCAG ACACGTGGCC CAATACTGGC AACAATCACA ATGACTGCTC TATCAATTGC
    TGTACGGCAG GCAATGGTAA CAGTGATAAC AACCTGACCA CCTACAGTCG CCCAGGGCAG
    CCCACTCCTT ATGCCACCAC CCAGCTCATC CAGTCAAACC TCAGCAACAA CATGAACAAT
    GGCAGCGGGG ACTCCAGCGA GAAGCACTGG AAACCTCCCG GCCAGCAGAA ACAAGAAGTG
    CCACCAGTTC AGTATAACAT CATGGAGCAA AACAAACTGA ACAAAGATTA TCGAGCAAAT
    GACACAGTTC CTCCAACTAT CCCATACAAC CAATCATATG ACCAGAATAC AGGAGGATCC
    TACAACAGTT CAGACCGGGG CAGTAGTACA TCTGGGAGTC AAGGGCACAA GAAAGGGGCA
    CGGACACCCA AAGTACCAAA ACAAGGTGGC ATGAACTGGG CAGACCTGCT TCCTCCTCCC
    CCAGCGCATC CTCCTCCTCA TAGCAATAGT GAAGAGTACA ATATTTCTGT AGATGAAAGC
    TATGACCAAG AAATGCCATG TCCCGTGCCA CCAGCAAGGA TGTATTTGCA ACAGAATGAA
    CTAGAAGAGG AGGAAGATGA GCGAGGCCCC ACTCCCCCTG TGCGGGGAGC AGCTTCTTCT
    CCAGCTGCTG TGTCTTATAG CCATCAGTCC ACTGCTACTC TGACCCCCTC CCCACAAGAA
    GAACTCCAGC CCATGTTGCA GGACTGCCCG GAGGAGATTG GCCACATGCA GCACCAACCC
    GATAGGAGAC GGCAGCCTGT GAGTCCTCCT CCACCACCAA GACCGATTTC CCCACCACAT
    ACCTATGGCT ACATTTCAGG ACCTCTTGTC TCAGATATGG ATACAGATGC ACCAGAAGAG
    GAAGAAGATG AAGCAGACAT GGAAGTGGCC AAGATGCAGA CCAGACGGCT TTTGTTACGT
    GGCCTTGAGC AGACCCCTGC CTCCAGTGTG GGGGACCTTG AAAGCTCTGT CACTGGGTCC
    ATGATCAATG GCTGGGGCTC AGCCTCAGAG GAGGACAACA TTTCCAGTGG ACGCTCCAGT
    GTGAGTTCTT CTGATGGCTC CTTCTTCACT GATGCTGATT TTGCCCAGGC AGTGGCAGCA
    GCTGCAGAAT ATGCTGGTCT TAAAGTGGCA CGTCGTCAGA TGCAGGATGC TGCTGGTCGC
    CGACATTTTC ATGCATCCCA GTGCCCTCGA CCCACAAGTC CTGTCTCTAC AGACAGCAAC
    ATGAGTGCTG CTGTAATGCA GAAGGCCAGA CCAGCCAAGA AGCAAAAACA CCAGCCAGGA
    CATCTGCGCA GAGAAGTCTA CACAGATGAT CTACCACCTC CTCCGGTGCC ACCACCTGCT
    ATAAAATCAC CCACTGTCCA ATCCAAGGCA CAGCTGGAGG TGCGACCTGT TATGGTGCCA
    AAACTCCCTT CTATAGAAGC AAGAACAGAC CGATCATCAG ATAGAAAAGT AGGCAGTTAC
    AAGGGGAGAG AAGTACTGGA TGGAAGACAG GTTGCTGATA TGAGAACCAA TCCAGGTGAT
    ACCAGAGAAG CACAGGAACA GCAAAATGAT GGAAAAGGAC GAGTAAACAA GGCAGCAAAA
    CGAGACCTTC CACCAGCAAA GACTCACCTC ATCCAAGAGG ATATCCTACC TTACTGTAGA
    CCTACTTTTC CGACATCAAA TAATCCCAGA GATCCTAGTT CTTCAAGCTC AATGTCATCA
    AGAGGATCAG GAAGCAGACA AAGAGAACAA GCAAATGTAG GTCGAAGAAA TATCGCAGAA
    ATGCAAGTTC TTGGAGGATA TGAAAGAGGA GAAGATAATA ATGAAGAATT AGAGGAAACT
    GAAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe283890
    Clone ID Related Accession (Same CDS sequence) XM_022413115.1
    Accession Version XM_022413115.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4857bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product roundabout homolog 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCTGAGAC CTCCGTGCAG CTCAGTATGC TGCAGGAGGG GCAAGGAGGA AGTGTGCTGT 
    GTGTTGTCCC TGTGTGTTTT TCTGTCACAT GTGGATTTAC TTGTGTCTGG ACATGCTGTG
    ATCGAAGTGG CAACTACCCT TACTACTGTG ACCCCATCAG CAGCTCTGGT GTTGTTTCCG
    ACAGACCCTG AAGACCTAGA GAGGGGGAAC GACAACGGGA CGCCAATCCC CACCTCTGAT
    AATGATGACA ATTCGCTGGG CTACACAGGC TCCCGTCTCC GTCAGGAAGA TTTTCCACCT
    CGTATTGTTG AACACCCTTC AGACCTAATT GTGTCAAAAG GAGAACCTGC AACTCTGAAC
    TGTAAAGCTG AAGGCCGCCC CACGCCCACC ATTGAATGGT ACAAAGGTGG GGAGAGAGTG
    GAGACAGACA AAGATGACCC TCGCTCACAC CGAATGTTGC TGCCAAGTGG ATCTTTATTT
    TTCTTACGTA TAGTACATGG ACGAAAAAGT AGGCCTGATG AAGGGGTCTA CGTCTGTGTG
    GCACGGAATT ACCTCGGAGA GGCTGTGAGC CATAATGCAT CGCTGGAGGT GGCTATACTA
    CGGGATGACT TCAGACAAAA TCCTTCTGAT GTCATGGTGG CAGTGGGGGA ACCTGCTGTG
    ATGGAATGCC AACCCCCACG AGGCCATCCT GAACCCACCA TTTCGTGGAA GAAAGATGGC
    TCTCCACTGG ATGATAAAGA TGAAAGAATA ACTATACGAG GTGGAAAGCT CATGATTACT
    TACACCCGCA AAAGTGATGC TGGCAAATAT GTTTGTGTTG GTACCAACAT GGTTGGGGAA
    CGTGAGAGTG AAGTAGCAGA GCTAACTGTC TTGGAGAGAC CATCATTTGT GAAGAGACCT
    AGTAACTTGG CTGTAACTGT GGATGACAGT GCAGAATTTA AATGTGAGGC TCGAGGTGAC
    CCTGTGCCTA CGGTACGATG GAGGAAAGAC GATGGAGAAC TGCCCAAATC CAGATACGAA
    ATCAGAGATG ATCATACTTT GAAGATTCGG AAAGTGATGG CTGGTGACAT GGGGTCATAT
    ACTTGTGTTG CAGAAAATAT GGTTGGAAAA GCTGAAGCAT CAGCTACTCT GACTGTCCAA
    GTTGGGTCTG AACCTCCACA TTTTGTTGTG AAACCTCGTG ACCAGGTTGT TGCCTTGGGG
    CGAACTGTAA CATTTCAGTG TGAAGCAACC GGAAATCCTC AACCAGCTAT TTTTTGGAGA
    AGAGAGGGGA GTCAGAATCT GCTTTTCTCA TATCAGCCGC CACAGTCAAC TAGCCGCTTT
    TCCGTCTCCC AGACTGGTGA TCTCACTATT ACTAATGTCC AGCGGTCTGA TGTTGGTTAT
    TATATCTGCC AGACTTTAAA TGTTGCCGGA AGTATCATCA CAAAAGCATA TTTGGAAGTT
    ACTGATGTGA TTGCAGACCG GCCTCCCCCA GTCATTCGAC AAGGTCCTGT GAATCAGACT
    GTAGCAGTGG ATGGTACTTT AGTGCTCAGC TGTGTGGCCA CAGGCAGTCC AATGCCCACG
    ATTCTCTGGA GAAAGGATGG AGTCCTTGTT TCAACCCAAG ATTCTAGAAT CAAACAGCTG
    GAGACGGGAG TGCTGCAGAT CCGATACGCT AAGCTGGGTG ACACAGGTCG GTACACCTGC
    ATTGCATCAA CCCCCAGTGG TGAAGCAACA TGGAGTGCTT ACATTGAAGT CCAAGAATTT
    GGAGTTCCAG TTCAGCCTCC ACGACCCACC GACCCAAATT TAATCCCTAG TGCCCCCTCA
    AAACCTGAAG TTACAGATGT CAGCAGAAAT ACAGTAACGT TGTCATGGCA ACCAAATTTG
    AATTCAGGTG CAACTCCAAC ATCTTATATT ATAGAAGCCT TCAGCCATGC ATCTGGTAGC
    AGCTGGCAGA CCGTAGCAGA GAATGTGAAA ACAGAAACAT TTGCCATTAA AGGACTCAAA
    CCTAATGCAA TTTACCTTTT CCTTGTGAGG GCAGCAAATG CCTATGGAAT TAGTGATCCA
    AGCCAGATAT CAGATCCAGT GAAAACACAA GATGTTCCCC CAACAAGTCA GGGAGTAGAT
    CACAAGCAGG TCCAGAGAGA ATTGGGAAAC GTCGTACTGC ACCTTCACAA CCCCACCATC
    CTCTCATCCT CTTCCATCGA AGTGCACTGG ACAGTGGACC AACAATCTCA GTATATTCAA
    GGATATAAAA TTCTTTATCG GCCATCTGGA GGCAACCATG GTGAATCGGA GTGGTTAGTT
    TTTGAAGTGA GGACTCCAAC CAAAAATAGC GTGGTTATCC CTGATCTCAA AAAGGGAGTC
    AACTATGAAA TTAAGGCTCG TCCTTTTTTT AATGAATTTC AAGGAGCAGA TAGTGAAATT
    AAATTTGCCA AAACCTTAGA AGAAGCACCC AGTGCCCCAC CCCAAAGTGT AACCGTATCA
    AAGAATGATG GAAATGGAAC TGCAATTCTT GTTAGTTGGC AGCCACCTCC AGAAGACACT
    CAAAATGGAA TGGTCCAAGA GTATAAGGTT TGGTGTCTGG GCAATGAAAC TCGTTATCAC
    ATAAACAAGA CGGTGGATGG TTCCACCTTT TCTGTGGTCA TTCCTTCTCT GGTTCCTGGG
    ATCCGATACA GCGTAGAAGT AGCAGCCAGC ACGGGAGCTG GAGCTGGGGT GAAGAGCGAG
    CCACAGTTCA TCCAGTTAGA TTCTCATGGA AACCCCGTGT CACCTGAAGA CCAAGTCAGC
    CTTGCTCAGC AGATCTCAGA TGTTGTGAAG CAGCCAGCGT TCATTGCGGG AATCGGGGCC
    GCCTGCTGGA TCATCCTCAT GGTCTTTAGC ATCTGGCTCT ATCGACACCG GAAGAAGAGA
    AATGGACTGA CTAGTACTTA TGCGGGTATC AGAAAAGTCC CGTCTTTTAC CTTCACACCA
    ACAGTAACTT ATCAGAGAGG AGGTGAAGCT GTGAGCAGTG GAGGGAGGCC AGGACTTCTG
    AACATCAGCG AACCTGCCAC ACAGCCCTGG CTGGCAGACA CGTGGCCCAA TACTGGCAAC
    AATCACAATG ACTGCTCTAT CAATTGCTGT ACGGCAGGCA ATGGGCAGCC CACTCCTTAT
    GCCACCACCC AGCTCATCCA GTCAAACCTC AGCAACAACA TGAACAATGG CAGCGGGGAC
    TCCAGCGAGA AGCACTGGAA ACCTCCCGGC CAGCAGAAAC AAGAAGTGCC ACCAGTTCAG
    TATAACATCA TGGAGCAAAA CAAACTGAAC AAAGATTATC GAGCAAATGA CACAGTTCCT
    CCAACTATCC CATACAACCA ATCATATGAC CAGAATACAG GAGGATCCTA CAACAGTTCA
    GACCGGGGCA GTAGTACATC TGGGAGTCAA GGGCACAAGA AAGGGGCACG GACACCCAAA
    GTACCAAAAC AAGGTGGCAT GAACTGGGCA GACCTGCTTC CTCCTCCCCC AGCGCATCCT
    CCTCCTCATA GCAATAGTGA AGAGTACAAT ATTTCTGTAG ATGAAAGCTA TGACCAAGAA
    ATGCCATGTC CCGTGCCACC AGCAAGGATG TATTTGCAAC AGAATGAACT AGAAGAGGAG
    GAAGATGAGC GAGGCCCCAC TCCCCCTGTG CGGGGAGCAG CTTCTTCTCC AGCTGCTGTG
    TCTTATAGCC ATCAGTCCAC TGCTACTCTG ACCCCCTCCC CACAAGAAGA ACTCCAGCCC
    ATGTTGCAGG ACTGCCCGGA GGAGATTGGC CACATGCAGC ACCAACCCGA TAGGAGACGG
    CAGCCTGTGA GTCCTCCTCC ACCACCAAGA CCGATTTCCC CACCACATAC CTATGGCTAC
    ATTTCAGGAC CTCTTGTCTC AGATATGGAT ACAGATGCAC CAGAAGAGGA AGAAGATGAA
    GCAGACATGG AAGTGGCCAA GATGCAGACC AGACGGCTTT TGTTACGTGG CCTTGAGCAG
    ACCCCTGCCT CCAGTGTGGG GGACCTTGAA AGCTCTGTCA CTGGGTCCAT GATCAATGGC
    TGGGGCTCAG CCTCAGAGGA GGACAACATT TCCAGTGGAC GCTCCAGTGT GAGTTCTTCT
    GATGGCTCCT TCTTCACTGA TGCTGATTTT GCCCAGGCAG TGGCAGCAGC TGCAGAATAT
    GCTGGTCTTA AAGTGGCACG TCGTCAGATG CAGGATGCTG CTGGTCGCCG ACATTTTCAT
    GCATCCCAGT GCCCTCGACC CACAAGTCCT GTCTCTACAG ACAGCAACAT GAGTGCTGCT
    GTAATGCAGA AGGCCAGACC AGCCAAGAAG CAAAAACACC AGCCAGGACA TCTGCGCAGA
    GAAGTCTACA CAGATGATCT ACCACCTCCT CCGGTGCCAC CACCTGCTAT AAAATCACCC
    ACTGTCCAAT CCAAGGCACA GCTGGAGGTG CGACCTGTTA TGGTGCCAAA ACTCCCTTCT
    ATAGAAGCAA GAACAGACCG ATCATCAGAT AGAAAAGTAG GCAGTTACAA GGGGAGAGAA
    GTACTGGATG GAAGACAGGT TGCTGATATG AGAACCAATC CAGGTGATAC CAGAGAAGCA
    CAGGAACAGC AAAATGATGG AAAAGGACGA GTAAACAAGG CAGCAAAACG AGACCTTCCA
    CCAGCAAAGA CTCACCTCAT CCAAGAGGAT ATCCTACCTT ACTGTAGACC TACTTTTCCG
    ACATCAAATA ATCCCAGAGA TCCTAGTTCT TCAAGCTCAA TGTCATCAAG AGGATCAGGA
    AGCAGACAAA GAGAACAAGC AAATGTAGGT CGAAGAAATA TCGCAGAAAT GCAAGTTCTT
    GGAGGATATG AAAGAGGAGA AGATAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268823.1
    CDS16..4872
    Translation

    Target ORF information:

    RefSeq Version XM_022413115.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413115.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCTGAGAC CTCCGTGCAG CTCAGTATGC TGCAGGAGGG GCAAGGAGGA AGTGTGCTGT 
    GTGTTGTCCC TGTGTGTTTT TCTGTCACAT GTGGATTTAC TTGTGTCTGG ACATGCTGTG
    ATCGAAGTGG CAACTACCCT TACTACTGTG ACCCCATCAG CAGCTCTGGT GTTGTTTCCG
    ACAGACCCTG AAGACCTAGA GAGGGGGAAC GACAACGGGA CGCCAATCCC CACCTCTGAT
    AATGATGACA ATTCGCTGGG CTACACAGGC TCCCGTCTCC GTCAGGAAGA TTTTCCACCT
    CGTATTGTTG AACACCCTTC AGACCTAATT GTGTCAAAAG GAGAACCTGC AACTCTGAAC
    TGTAAAGCTG AAGGCCGCCC CACGCCCACC ATTGAATGGT ACAAAGGTGG GGAGAGAGTG
    GAGACAGACA AAGATGACCC TCGCTCACAC CGAATGTTGC TGCCAAGTGG ATCTTTATTT
    TTCTTACGTA TAGTACATGG ACGAAAAAGT AGGCCTGATG AAGGGGTCTA CGTCTGTGTG
    GCACGGAATT ACCTCGGAGA GGCTGTGAGC CATAATGCAT CGCTGGAGGT GGCTATACTA
    CGGGATGACT TCAGACAAAA TCCTTCTGAT GTCATGGTGG CAGTGGGGGA ACCTGCTGTG
    ATGGAATGCC AACCCCCACG AGGCCATCCT GAACCCACCA TTTCGTGGAA GAAAGATGGC
    TCTCCACTGG ATGATAAAGA TGAAAGAATA ACTATACGAG GTGGAAAGCT CATGATTACT
    TACACCCGCA AAAGTGATGC TGGCAAATAT GTTTGTGTTG GTACCAACAT GGTTGGGGAA
    CGTGAGAGTG AAGTAGCAGA GCTAACTGTC TTGGAGAGAC CATCATTTGT GAAGAGACCT
    AGTAACTTGG CTGTAACTGT GGATGACAGT GCAGAATTTA AATGTGAGGC TCGAGGTGAC
    CCTGTGCCTA CGGTACGATG GAGGAAAGAC GATGGAGAAC TGCCCAAATC CAGATACGAA
    ATCAGAGATG ATCATACTTT GAAGATTCGG AAAGTGATGG CTGGTGACAT GGGGTCATAT
    ACTTGTGTTG CAGAAAATAT GGTTGGAAAA GCTGAAGCAT CAGCTACTCT GACTGTCCAA
    GTTGGGTCTG AACCTCCACA TTTTGTTGTG AAACCTCGTG ACCAGGTTGT TGCCTTGGGG
    CGAACTGTAA CATTTCAGTG TGAAGCAACC GGAAATCCTC AACCAGCTAT TTTTTGGAGA
    AGAGAGGGGA GTCAGAATCT GCTTTTCTCA TATCAGCCGC CACAGTCAAC TAGCCGCTTT
    TCCGTCTCCC AGACTGGTGA TCTCACTATT ACTAATGTCC AGCGGTCTGA TGTTGGTTAT
    TATATCTGCC AGACTTTAAA TGTTGCCGGA AGTATCATCA CAAAAGCATA TTTGGAAGTT
    ACTGATGTGA TTGCAGACCG GCCTCCCCCA GTCATTCGAC AAGGTCCTGT GAATCAGACT
    GTAGCAGTGG ATGGTACTTT AGTGCTCAGC TGTGTGGCCA CAGGCAGTCC AATGCCCACG
    ATTCTCTGGA GAAAGGATGG AGTCCTTGTT TCAACCCAAG ATTCTAGAAT CAAACAGCTG
    GAGACGGGAG TGCTGCAGAT CCGATACGCT AAGCTGGGTG ACACAGGTCG GTACACCTGC
    ATTGCATCAA CCCCCAGTGG TGAAGCAACA TGGAGTGCTT ACATTGAAGT CCAAGAATTT
    GGAGTTCCAG TTCAGCCTCC ACGACCCACC GACCCAAATT TAATCCCTAG TGCCCCCTCA
    AAACCTGAAG TTACAGATGT CAGCAGAAAT ACAGTAACGT TGTCATGGCA ACCAAATTTG
    AATTCAGGTG CAACTCCAAC ATCTTATATT ATAGAAGCCT TCAGCCATGC ATCTGGTAGC
    AGCTGGCAGA CCGTAGCAGA GAATGTGAAA ACAGAAACAT TTGCCATTAA AGGACTCAAA
    CCTAATGCAA TTTACCTTTT CCTTGTGAGG GCAGCAAATG CCTATGGAAT TAGTGATCCA
    AGCCAGATAT CAGATCCAGT GAAAACACAA GATGTTCCCC CAACAAGTCA GGGAGTAGAT
    CACAAGCAGG TCCAGAGAGA ATTGGGAAAC GTCGTACTGC ACCTTCACAA CCCCACCATC
    CTCTCATCCT CTTCCATCGA AGTGCACTGG ACAGTGGACC AACAATCTCA GTATATTCAA
    GGATATAAAA TTCTTTATCG GCCATCTGGA GGCAACCATG GTGAATCGGA GTGGTTAGTT
    TTTGAAGTGA GGACTCCAAC CAAAAATAGC GTGGTTATCC CTGATCTCAA AAAGGGAGTC
    AACTATGAAA TTAAGGCTCG TCCTTTTTTT AATGAATTTC AAGGAGCAGA TAGTGAAATT
    AAATTTGCCA AAACCTTAGA AGAAGCACCC AGTGCCCCAC CCCAAAGTGT AACCGTATCA
    AAGAATGATG GAAATGGAAC TGCAATTCTT GTTAGTTGGC AGCCACCTCC AGAAGACACT
    CAAAATGGAA TGGTCCAAGA GTATAAGGTT TGGTGTCTGG GCAATGAAAC TCGTTATCAC
    ATAAACAAGA CGGTGGATGG TTCCACCTTT TCTGTGGTCA TTCCTTCTCT GGTTCCTGGG
    ATCCGATACA GCGTAGAAGT AGCAGCCAGC ACGGGAGCTG GAGCTGGGGT GAAGAGCGAG
    CCACAGTTCA TCCAGTTAGA TTCTCATGGA AACCCCGTGT CACCTGAAGA CCAAGTCAGC
    CTTGCTCAGC AGATCTCAGA TGTTGTGAAG CAGCCAGCGT TCATTGCGGG AATCGGGGCC
    GCCTGCTGGA TCATCCTCAT GGTCTTTAGC ATCTGGCTCT ATCGACACCG GAAGAAGAGA
    AATGGACTGA CTAGTACTTA TGCGGGTATC AGAAAAGTCC CGTCTTTTAC CTTCACACCA
    ACAGTAACTT ATCAGAGAGG AGGTGAAGCT GTGAGCAGTG GAGGGAGGCC AGGACTTCTG
    AACATCAGCG AACCTGCCAC ACAGCCCTGG CTGGCAGACA CGTGGCCCAA TACTGGCAAC
    AATCACAATG ACTGCTCTAT CAATTGCTGT ACGGCAGGCA ATGGGCAGCC CACTCCTTAT
    GCCACCACCC AGCTCATCCA GTCAAACCTC AGCAACAACA TGAACAATGG CAGCGGGGAC
    TCCAGCGAGA AGCACTGGAA ACCTCCCGGC CAGCAGAAAC AAGAAGTGCC ACCAGTTCAG
    TATAACATCA TGGAGCAAAA CAAACTGAAC AAAGATTATC GAGCAAATGA CACAGTTCCT
    CCAACTATCC CATACAACCA ATCATATGAC CAGAATACAG GAGGATCCTA CAACAGTTCA
    GACCGGGGCA GTAGTACATC TGGGAGTCAA GGGCACAAGA AAGGGGCACG GACACCCAAA
    GTACCAAAAC AAGGTGGCAT GAACTGGGCA GACCTGCTTC CTCCTCCCCC AGCGCATCCT
    CCTCCTCATA GCAATAGTGA AGAGTACAAT ATTTCTGTAG ATGAAAGCTA TGACCAAGAA
    ATGCCATGTC CCGTGCCACC AGCAAGGATG TATTTGCAAC AGAATGAACT AGAAGAGGAG
    GAAGATGAGC GAGGCCCCAC TCCCCCTGTG CGGGGAGCAG CTTCTTCTCC AGCTGCTGTG
    TCTTATAGCC ATCAGTCCAC TGCTACTCTG ACCCCCTCCC CACAAGAAGA ACTCCAGCCC
    ATGTTGCAGG ACTGCCCGGA GGAGATTGGC CACATGCAGC ACCAACCCGA TAGGAGACGG
    CAGCCTGTGA GTCCTCCTCC ACCACCAAGA CCGATTTCCC CACCACATAC CTATGGCTAC
    ATTTCAGGAC CTCTTGTCTC AGATATGGAT ACAGATGCAC CAGAAGAGGA AGAAGATGAA
    GCAGACATGG AAGTGGCCAA GATGCAGACC AGACGGCTTT TGTTACGTGG CCTTGAGCAG
    ACCCCTGCCT CCAGTGTGGG GGACCTTGAA AGCTCTGTCA CTGGGTCCAT GATCAATGGC
    TGGGGCTCAG CCTCAGAGGA GGACAACATT TCCAGTGGAC GCTCCAGTGT GAGTTCTTCT
    GATGGCTCCT TCTTCACTGA TGCTGATTTT GCCCAGGCAG TGGCAGCAGC TGCAGAATAT
    GCTGGTCTTA AAGTGGCACG TCGTCAGATG CAGGATGCTG CTGGTCGCCG ACATTTTCAT
    GCATCCCAGT GCCCTCGACC CACAAGTCCT GTCTCTACAG ACAGCAACAT GAGTGCTGCT
    GTAATGCAGA AGGCCAGACC AGCCAAGAAG CAAAAACACC AGCCAGGACA TCTGCGCAGA
    GAAGTCTACA CAGATGATCT ACCACCTCCT CCGGTGCCAC CACCTGCTAT AAAATCACCC
    ACTGTCCAAT CCAAGGCACA GCTGGAGGTG CGACCTGTTA TGGTGCCAAA ACTCCCTTCT
    ATAGAAGCAA GAACAGACCG ATCATCAGAT AGAAAAGTAG GCAGTTACAA GGGGAGAGAA
    GTACTGGATG GAAGACAGGT TGCTGATATG AGAACCAATC CAGGTGATAC CAGAGAAGCA
    CAGGAACAGC AAAATGATGG AAAAGGACGA GTAAACAAGG CAGCAAAACG AGACCTTCCA
    CCAGCAAAGA CTCACCTCAT CCAAGAGGAT ATCCTACCTT ACTGTAGACC TACTTTTCCG
    ACATCAAATA ATCCCAGAGA TCCTAGTTCT TCAAGCTCAA TGTCATCAAG AGGATCAGGA
    AGCAGACAAA GAGAACAAGC AAATGTAGGT CGAAGAAATA TCGCAGAAAT GCAAGTTCTT
    GGAGGATATG AAAGAGGAGA AGATAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44463
    Clone ID Related Accession (Same CDS sequence) XM_014109750.1
    Accession Version XM_014109750.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4965bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product roundabout homolog 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA AACATGTTCC TTTTTTGGTC ATAATATCAC TCCTCAGCTT GTCCAAAGAT 
    CACCTGTTCC TGGCGCAGCT TATTCCCGAC CCTGAAGACC TAGAGAGGGG GAACGACAAC
    GGGACGCCAA TCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTCCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CTTCAGACCT AATTGTGTCA
    AAAGGAGAAC CTGCAACTCT GAACTGTAAA GCTGAAGGCC GCCCCACGCC CACCATTGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTGGCACGG AATTACCTCG GAGAGGCTGT GAGCCATAAT
    GCATCGCTGG AGGTGGCTAT ACTACGGGAT GACTTCAGAC AAAATCCTTC TGATGTCATG
    GTGGCAGTGG GGGAACCTGC TGTGATGGAA TGCCAACCCC CACGAGGCCA TCCTGAACCC
    ACCATTTCGT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGTGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCTGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAACGTGAG AGTGAAGTAG CAGAGCTAAC TGTCTTGGAG
    AGACCATCAT TTGTGAAGAG ACCTAGTAAC TTGGCTGTAA CTGTGGATGA CAGTGCAGAA
    TTTAAATGTG AGGCTCGAGG TGACCCTGTG CCTACGGTAC GATGGAGGAA AGACGATGGA
    GAACTGCCCA AATCCAGATA CGAAATCAGA GATGATCATA CTTTGAAGAT TCGGAAAGTG
    ATGGCTGGTG ACATGGGGTC ATATACTTGT GTTGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCAGCTA CTCTGACTGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCT
    CGTGACCAGG TTGTTGCCTT GGGGCGAACT GTAACATTTC AGTGTGAAGC AACCGGAAAT
    CCTCAACCAG CTATTTTTTG GAGAAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCGCCACAGT CAACTAGCCG CTTTTCCGTC TCCCAGACTG GTGATCTCAC TATTACTAAT
    GTCCAGCGGT CTGATGTTGG TTATTATATC TGCCAGACTT TAAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAATGCC CACGATTCTC TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTA GAATCAAACA GCTGGAGACG GGAGTGCTGC AGATCCGATA CGCTAAGCTG
    GGTGACACAG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCACGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCAG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CAACATCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCA
    AATGCCTATG GAATTAGTGA TCCAAGCCAG ATATCAGATC CAGTGAAAAC ACAAGATGTT
    CCCCCAACAA GTCAGGGAGT AGATCACAAG CAGGTCCAGA GAGAATTGGG AAACGTCGTA
    CTGCACCTTC ACAACCCCAC CATCCTCTCA TCCTCTTCCA TCGAAGTGCA CTGGACAGTG
    GACCAACAAT CTCAGTATAT TCAAGGATAT AAAATTCTTT ATCGGCCATC TGGAGGCAAC
    CATGGTGAAT CGGAGTGGTT AGTTTTTGAA GTGAGGACTC CAACCAAAAA TAGCGTGGTT
    ATCCCTGATC TCAAAAAGGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCT TAGAAGAAGC ACCCAGTGCC
    CCACCCCAAA GTGTAACCGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT
    CTGGGCAATG AAACTCGTTA TCACATAAAC AAGACGGTGG ATGGTTCCAC CTTTTCTGTG
    GTCATTCCTT CTCTGGTTCC TGGGATCCGA TACAGCGTAG AAGTAGCAGC CAGCACGGGA
    GCTGGAGCTG GGGTGAAGAG CGAGCCACAG TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AAGACCAAGT CAGCCTTGCT CAGCAGATCT CAGATGTTGT GAAGCAGCCA
    GCGTTCATTG CGGGAATCGG GGCCGCCTGC TGGATCATCC TCATGGTCTT TAGCATCTGG
    CTCTATCGAC ACCGGAAGAA GAGAAATGGA CTGACTAGTA CTTATGCGGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGTGA AGCTGTGAGC
    AGTGGAGGGA GGCCAGGACT TCTGAACATC AGCGAACCTG CCACACAGCC CTGGCTGGCA
    GACACGTGGC CCAATACTGG CAACAATCAC AATGACTGCT CTATCAATTG CTGTACGGCA
    GGCAATGGTA ACAGTGATAA CAACCTGACC ACCTACAGTC GCCCAGCCGA TTGTATAGCA
    AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT
    TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT
    CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCCA CTCCTTATGC CACCACCCAG
    CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GCGGGGACTC CAGCGAGAAG
    CACTGGAAAC CTCCCGGCCA GCAGAAACAA GAAGTGCCAC CAGTTCAGTA TAACATCATG
    GAGCAAAACA AACTGAACAA AGATTATCGA GCAAATGACA CAGTTCCTCC AACTATCCCA
    TACAACCAAT CATATGACCA GAATACAGGA GGATCCTACA ACAGTTCAGA CCGGGGCAGT
    AGTACATCTG GGAGTCAAGG GCACAAGAAA GGGGCACGGA CACCCAAAGT ACCAAAACAA
    GGTGGCATGA ACTGGGCAGA CCTGCTTCCT CCTCCCCCAG CGCATCCTCC TCCTCATAGC
    AATAGTGAAG AGTACAATAT TTCTGTAGAT GAAAGCTATG ACCAAGAAAT GCCATGTCCC
    GTGCCACCAG CAAGGATGTA TTTGCAACAG AATGAACTAG AAGAGGAGGA AGATGAGCGA
    GGCCCCACTC CCCCTGTGCG GGGAGCAGCT TCTTCTCCAG CTGCTGTGTC TTATAGCCAT
    CAGTCCACTG CTACTCTGAC CCCCTCCCCA CAAGAAGAAC TCCAGCCCAT GTTGCAGGAC
    TGCCCGGAGG AGATTGGCCA CATGCAGCAC CAACCCGATA GGAGACGGCA GCCTGTGAGT
    CCTCCTCCAC CACCAAGACC GATTTCCCCA CCACATACCT ATGGCTACAT TTCAGGACCT
    CTTGTCTCAG ATATGGATAC AGATGCACCA GAAGAGGAAG AAGATGAAGC AGACATGGAA
    GTGGCCAAGA TGCAGACCAG ACGGCTTTTG TTACGTGGCC TTGAGCAGAC CCCTGCCTCC
    AGTGTGGGGG ACCTTGAAAG CTCTGTCACT GGGTCCATGA TCAATGGCTG GGGCTCAGCC
    TCAGAGGAGG ACAACATTTC CAGTGGACGC TCCAGTGTGA GTTCTTCTGA TGGCTCCTTC
    TTCACTGATG CTGATTTTGC CCAGGCAGTG GCAGCAGCTG CAGAATATGC TGGTCTTAAA
    GTGGCACGTC GTCAGATGCA GGATGCTGCT GGTCGCCGAC ATTTTCATGC ATCCCAGTGC
    CCTCGACCCA CAAGTCCTGT CTCTACAGAC AGCAACATGA GTGCTGCTGT AATGCAGAAG
    GCCAGACCAG CCAAGAAGCA AAAACACCAG CCAGGACATC TGCGCAGAGA AGTCTACACA
    GATGATCTAC CACCTCCTCC GGTGCCACCA CCTGCTATAA AATCACCCAC TGTCCAATCC
    AAGGCACAGC TGGAGGTGCG ACCTGTTATG GTGCCAAAAC TCCCTTCTAT AGAAGCAAGA
    ACAGACCGAT CATCAGATAG AAAAGTAGGC AGTTACAAGG GGAGAGAAGT ACTGGATGGA
    AGACAGGTTG CTGATATGAG AACCAATCCA GGTGATACCA GAGAAGCACA GGAACAGCAA
    AATGATGGAA AAGGACGAGT AAACAAGGCA GCAAAACGAG ACCTTCCACC AGCAAAGACT
    CACCTCATCC AAGAGGATAT CCTACCTTAC TGTAGACCTA CTTTTCCGAC ATCAAATAAT
    CCCAGAGATC CTAGTTCTTC AAGCTCAATG TCATCAAGAG GATCAGGAAG CAGACAAAGA
    GAACAAGCAA ATGTAGGTCG AAGAAATATC GCAGAAATGC AAGTTCTTGG AGGATATGAA
    AGAGGAGAAG ATAATAATGA AGAATTAGAG GAAACTGAAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965225.1
    CDS303..5267
    Translation

    Target ORF information:

    RefSeq Version XM_014109750.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109750.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA AACATGTTCC TTTTTTGGTC ATAATATCAC TCCTCAGCTT GTCCAAAGAT 
    CACCTGTTCC TGGCGCAGCT TATTCCCGAC CCTGAAGACC TAGAGAGGGG GAACGACAAC
    GGGACGCCAA TCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTCCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CTTCAGACCT AATTGTGTCA
    AAAGGAGAAC CTGCAACTCT GAACTGTAAA GCTGAAGGCC GCCCCACGCC CACCATTGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTGGCACGG AATTACCTCG GAGAGGCTGT GAGCCATAAT
    GCATCGCTGG AGGTGGCTAT ACTACGGGAT GACTTCAGAC AAAATCCTTC TGATGTCATG
    GTGGCAGTGG GGGAACCTGC TGTGATGGAA TGCCAACCCC CACGAGGCCA TCCTGAACCC
    ACCATTTCGT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGTGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCTGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAACGTGAG AGTGAAGTAG CAGAGCTAAC TGTCTTGGAG
    AGACCATCAT TTGTGAAGAG ACCTAGTAAC TTGGCTGTAA CTGTGGATGA CAGTGCAGAA
    TTTAAATGTG AGGCTCGAGG TGACCCTGTG CCTACGGTAC GATGGAGGAA AGACGATGGA
    GAACTGCCCA AATCCAGATA CGAAATCAGA GATGATCATA CTTTGAAGAT TCGGAAAGTG
    ATGGCTGGTG ACATGGGGTC ATATACTTGT GTTGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCAGCTA CTCTGACTGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCT
    CGTGACCAGG TTGTTGCCTT GGGGCGAACT GTAACATTTC AGTGTGAAGC AACCGGAAAT
    CCTCAACCAG CTATTTTTTG GAGAAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCGCCACAGT CAACTAGCCG CTTTTCCGTC TCCCAGACTG GTGATCTCAC TATTACTAAT
    GTCCAGCGGT CTGATGTTGG TTATTATATC TGCCAGACTT TAAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAATGCC CACGATTCTC TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTA GAATCAAACA GCTGGAGACG GGAGTGCTGC AGATCCGATA CGCTAAGCTG
    GGTGACACAG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCACGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCAG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CAACATCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCA
    AATGCCTATG GAATTAGTGA TCCAAGCCAG ATATCAGATC CAGTGAAAAC ACAAGATGTT
    CCCCCAACAA GTCAGGGAGT AGATCACAAG CAGGTCCAGA GAGAATTGGG AAACGTCGTA
    CTGCACCTTC ACAACCCCAC CATCCTCTCA TCCTCTTCCA TCGAAGTGCA CTGGACAGTG
    GACCAACAAT CTCAGTATAT TCAAGGATAT AAAATTCTTT ATCGGCCATC TGGAGGCAAC
    CATGGTGAAT CGGAGTGGTT AGTTTTTGAA GTGAGGACTC CAACCAAAAA TAGCGTGGTT
    ATCCCTGATC TCAAAAAGGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCT TAGAAGAAGC ACCCAGTGCC
    CCACCCCAAA GTGTAACCGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT
    CTGGGCAATG AAACTCGTTA TCACATAAAC AAGACGGTGG ATGGTTCCAC CTTTTCTGTG
    GTCATTCCTT CTCTGGTTCC TGGGATCCGA TACAGCGTAG AAGTAGCAGC CAGCACGGGA
    GCTGGAGCTG GGGTGAAGAG CGAGCCACAG TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AAGACCAAGT CAGCCTTGCT CAGCAGATCT CAGATGTTGT GAAGCAGCCA
    GCGTTCATTG CGGGAATCGG GGCCGCCTGC TGGATCATCC TCATGGTCTT TAGCATCTGG
    CTCTATCGAC ACCGGAAGAA GAGAAATGGA CTGACTAGTA CTTATGCGGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGTGA AGCTGTGAGC
    AGTGGAGGGA GGCCAGGACT TCTGAACATC AGCGAACCTG CCACACAGCC CTGGCTGGCA
    GACACGTGGC CCAATACTGG CAACAATCAC AATGACTGCT CTATCAATTG CTGTACGGCA
    GGCAATGGTA ACAGTGATAA CAACCTGACC ACCTACAGTC GCCCAGCCGA TTGTATAGCA
    AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT
    TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT
    CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCCA CTCCTTATGC CACCACCCAG
    CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GCGGGGACTC CAGCGAGAAG
    CACTGGAAAC CTCCCGGCCA GCAGAAACAA GAAGTGCCAC CAGTTCAGTA TAACATCATG
    GAGCAAAACA AACTGAACAA AGATTATCGA GCAAATGACA CAGTTCCTCC AACTATCCCA
    TACAACCAAT CATATGACCA GAATACAGGA GGATCCTACA ACAGTTCAGA CCGGGGCAGT
    AGTACATCTG GGAGTCAAGG GCACAAGAAA GGGGCACGGA CACCCAAAGT ACCAAAACAA
    GGTGGCATGA ACTGGGCAGA CCTGCTTCCT CCTCCCCCAG CGCATCCTCC TCCTCATAGC
    AATAGTGAAG AGTACAATAT TTCTGTAGAT GAAAGCTATG ACCAAGAAAT GCCATGTCCC
    GTGCCACCAG CAAGGATGTA TTTGCAACAG AATGAACTAG AAGAGGAGGA AGATGAGCGA
    GGCCCCACTC CCCCTGTGCG GGGAGCAGCT TCTTCTCCAG CTGCTGTGTC TTATAGCCAT
    CAGTCCACTG CTACTCTGAC CCCCTCCCCA CAAGAAGAAC TCCAGCCCAT GTTGCAGGAC
    TGCCCGGAGG AGATTGGCCA CATGCAGCAC CAACCCGATA GGAGACGGCA GCCTGTGAGT
    CCTCCTCCAC CACCAAGACC GATTTCCCCA CCACATACCT ATGGCTACAT TTCAGGACCT
    CTTGTCTCAG ATATGGATAC AGATGCACCA GAAGAGGAAG AAGATGAAGC AGACATGGAA
    GTGGCCAAGA TGCAGACCAG ACGGCTTTTG TTACGTGGCC TTGAGCAGAC CCCTGCCTCC
    AGTGTGGGGG ACCTTGAAAG CTCTGTCACT GGGTCCATGA TCAATGGCTG GGGCTCAGCC
    TCAGAGGAGG ACAACATTTC CAGTGGACGC TCCAGTGTGA GTTCTTCTGA TGGCTCCTTC
    TTCACTGATG CTGATTTTGC CCAGGCAGTG GCAGCAGCTG CAGAATATGC TGGTCTTAAA
    GTGGCACGTC GTCAGATGCA GGATGCTGCT GGTCGCCGAC ATTTTCATGC ATCCCAGTGC
    CCTCGACCCA CAAGTCCTGT CTCTACAGAC AGCAACATGA GTGCTGCTGT AATGCAGAAG
    GCCAGACCAG CCAAGAAGCA AAAACACCAG CCAGGACATC TGCGCAGAGA AGTCTACACA
    GATGATCTAC CACCTCCTCC GGTGCCACCA CCTGCTATAA AATCACCCAC TGTCCAATCC
    AAGGCACAGC TGGAGGTGCG ACCTGTTATG GTGCCAAAAC TCCCTTCTAT AGAAGCAAGA
    ACAGACCGAT CATCAGATAG AAAAGTAGGC AGTTACAAGG GGAGAGAAGT ACTGGATGGA
    AGACAGGTTG CTGATATGAG AACCAATCCA GGTGATACCA GAGAAGCACA GGAACAGCAA
    AATGATGGAA AAGGACGAGT AAACAAGGCA GCAAAACGAG ACCTTCCACC AGCAAAGACT
    CACCTCATCC AAGAGGATAT CCTACCTTAC TGTAGACCTA CTTTTCCGAC ATCAAATAAT
    CCCAGAGATC CTAGTTCTTC AAGCTCAATG TCATCAAGAG GATCAGGAAG CAGACAAAGA
    GAACAAGCAA ATGTAGGTCG AAGAAATATC GCAGAAATGC AAGTTCTTGG AGGATATGAA
    AGAGGAGAAG ATAATAATGA AGAATTAGAG GAAACTGAAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44464
    Clone ID Related Accession (Same CDS sequence) XM_014109751.1
    Accession Version XM_014109751.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product roundabout homolog 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA AACATGTTCC TTTTTTGGTC ATAATATCAC TCCTCAGCTT GTCCAAAGAT 
    CACCTGTTCC TGGCGCAGCT TATTCCCGAC CCTGAAGACC TAGAGAGGGG GAACGACAAC
    GGGACGCCAA TCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTCCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CTTCAGACCT AATTGTGTCA
    AAAGGAGAAC CTGCAACTCT GAACTGTAAA GCTGAAGGCC GCCCCACGCC CACCATTGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTGGCACGG AATTACCTCG GAGAGGCTGT GAGCCATAAT
    GCATCGCTGG AGGTGGCTAT ACTACGGGAT GACTTCAGAC AAAATCCTTC TGATGTCATG
    GTGGCAGTGG GGGAACCTGC TGTGATGGAA TGCCAACCCC CACGAGGCCA TCCTGAACCC
    ACCATTTCGT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGTGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCTGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAACGTGAG AGTGAAGTAG CAGAGCTAAC TGTCTTGGAG
    AGACCATCAT TTGTGAAGAG ACCTAGTAAC TTGGCTGTAA CTGTGGATGA CAGTGCAGAA
    TTTAAATGTG AGGCTCGAGG TGACCCTGTG CCTACGGTAC GATGGAGGAA AGACGATGGA
    GAACTGCCCA AATCCAGATA CGAAATCAGA GATGATCATA CTTTGAAGAT TCGGAAAGTG
    ATGGCTGGTG ACATGGGGTC ATATACTTGT GTTGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCAGCTA CTCTGACTGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCT
    CGTGACCAGG TTGTTGCCTT GGGGCGAACT GTAACATTTC AGTGTGAAGC AACCGGAAAT
    CCTCAACCAG CTATTTTTTG GAGAAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCGCCACAGT CAACTAGCCG CTTTTCCGTC TCCCAGACTG GTGATCTCAC TATTACTAAT
    GTCCAGCGGT CTGATGTTGG TTATTATATC TGCCAGACTT TAAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAATGCC CACGATTCTC TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTA GAATCAAACA GCTGGAGACG GGAGTGCTGC AGATCCGATA CGCTAAGCTG
    GGTGACACAG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCACGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCAG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CAACATCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCA
    AATGCCTATG GAATTAGTGA TCCAAGCCAG ATATCAGATC CAGTGAAAAC ACAAGATGTT
    CCCCCAACAA GTCAGGGAGT AGATCACAAG CAGGTCCAGA GAGAATTGGG AAACGTCGTA
    CTGCACCTTC ACAACCCCAC CATCCTCTCA TCCTCTTCCA TCGAAGTGCA CTGGACAGTG
    GACCAACAAT CTCAGTATAT TCAAGGATAT AAAATTCTTT ATCGGCCATC TGGAGGCAAC
    CATGGTGAAT CGGAGTGGTT AGTTTTTGAA GTGAGGACTC CAACCAAAAA TAGCGTGGTT
    ATCCCTGATC TCAAAAAGGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCT TAGAAGAAGC ACCCAGTGCC
    CCACCCCAAA GTGTAACCGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT
    CTGGGCAATG AAACTCGTTA TCACATAAAC AAGACGGTGG ATGGTTCCAC CTTTTCTGTG
    GTCATTCCTT CTCTGGTTCC TGGGATCCGA TACAGCGTAG AAGTAGCAGC CAGCACGGGA
    GCTGGAGCTG GGGTGAAGAG CGAGCCACAG TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AAGACCAAGT CAGCCTTGCT CAGCAGATCT CAGATGTTGT GAAGCAGCCA
    GCGTTCATTG CGGGAATCGG GGCCGCCTGC TGGATCATCC TCATGGTCTT TAGCATCTGG
    CTCTATCGAC ACCGGAAGAA GAGAAATGGA CTGACTAGTA CTTATGCGGG TATCAGAAAA
    GTAACTTATC AGAGAGGAGG TGAAGCTGTG AGCAGTGGAG GGAGGCCAGG ACTTCTGAAC
    ATCAGCGAAC CTGCCACACA GCCCTGGCTG GCAGACACGT GGCCCAATAC TGGCAACAAT
    CACAATGACT GCTCTATCAA TTGCTGTACG GCAGGCAATG GTAACAGTGA TAACAACCTG
    ACCACCTACA GTCGCCCAGC CGATTGTATA GCAAATTATA ACAACCAACT GGATAACAAA
    CAAACAAATC TGATGCTCCC TGAGTCAACT GTTTATGGTG ATGTGGACCT TAGTAACAAA
    ATCAATGAGA TGAAAACCTT CAATAGCCCA AATCTGAAGG ATGGGCGTTT TGTCAATCCA
    TCAGGGCAGC CCACTCCTTA TGCCACCACC CAGCTCATCC AGTCAAACCT CAGCAACAAC
    ATGAACAATG GCAGCGGGGA CTCCAGCGAG AAGCACTGGA AACCTCCCGG CCAGCAGAAA
    CAAGAAGTGC CACCAGTTCA GTATAACATC ATGGAGCAAA ACAAACTGAA CAAAGATTAT
    CGAGCAAATG ACACAGTTCC TCCAACTATC CCATACAACC AATCATATGA CCAGAATACA
    GGAGGATCCT ACAACAGTTC AGACCGGGGC AGTAGTACAT CTGGGAGTCA AGGGCACAAG
    AAAGGGGCAC GGACACCCAA AGTACCAAAA CAAGGTGGCA TGAACTGGGC AGACCTGCTT
    CCTCCTCCCC CAGCGCATCC TCCTCCTCAT AGCAATAGTG AAGAGTACAA TATTTCTGTA
    GATGAAAGCT ATGACCAAGA AATGCCATGT CCCGTGCCAC CAGCAAGGAT GTATTTGCAA
    CAGAATGAAC TAGAAGAGGA GGAAGATGAG CGAGGCCCCA CTCCCCCTGT GCGGGGAGCA
    GCTTCTTCTC CAGCTGCTGT GTCTTATAGC CATCAGTCCA CTGCTACTCT GACCCCCTCC
    CCACAAGAAG AACTCCAGCC CATGTTGCAG GACTGCCCGG AGGAGATTGG CCACATGCAG
    CACCAACCCG ATAGGAGACG GCAGCCTGTG AGTCCTCCTC CACCACCAAG ACCGATTTCC
    CCACCACATA CCTATGGCTA CATTTCAGGA CCTCTTGTCT CAGATATGGA TACAGATGCA
    CCAGAAGAGG AAGAAGATGA AGCAGACATG GAAGTGGCCA AGATGCAGAC CAGACGGCTT
    TTGTTACGTG GCCTTGAGCA GACCCCTGCC TCCAGTGTGG GGGACCTTGA AAGCTCTGTC
    ACTGGGTCCA TGATCAATGG CTGGGGCTCA GCCTCAGAGG AGGACAACAT TTCCAGTGGA
    CGCTCCAGTG TGAGTTCTTC TGATGGCTCC TTCTTCACTG ATGCTGATTT TGCCCAGGCA
    GTGGCAGCAG CTGCAGAATA TGCTGGTCTT AAAGTGGCAC GTCGTCAGAT GCAGGATGCT
    GCTGGTCGCC GACATTTTCA TGCATCCCAG TGCCCTCGAC CCACAAGTCC TGTCTCTACA
    GACAGCAACA TGAGTGCTGC TGTAATGCAG AAGGCCAGAC CAGCCAAGAA GCAAAAACAC
    CAGCCAGGAC ATCTGCGCAG AGAAGTCTAC ACAGATGATC TACCACCTCC TCCGGTGCCA
    CCACCTGCTA TAAAATCACC CACTGTCCAA TCCAAGGCAC AGCTGGAGGT GCGACCTGTT
    ATGGTGCCAA AACTCCCTTC TATAGAAGCA AGAACAGACC GATCATCAGA TAGAAAAGTA
    GGCAGTTACA AGGGGAGAGA AGTACTGGAT GGAAGACAGG TTGCTGATAT GAGAACCAAT
    CCAGGTGATA CCAGAGAAGC ACAGGAACAG CAAAATGATG GAAAAGGACG AGTAAACAAG
    GCAGCAAAAC GAGACCTTCC ACCAGCAAAG ACTCACCTCA TCCAAGAGGA TATCCTACCT
    TACTGTAGAC CTACTTTTCC GACATCAAAT AATCCCAGAG ATCCTAGTTC TTCAAGCTCA
    ATGTCATCAA GAGGATCAGG AAGCAGACAA AGAGAACAAG CAAATGTAGG TCGAAGAAAT
    ATCGCAGAAA TGCAAGTTCT TGGAGGATAT GAAAGAGGAG AAGATAATAA TGAAGAATTA
    GAGGAAACTG AAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965226.1
    CDS303..5240
    Translation

    Target ORF information:

    RefSeq Version XM_014109751.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109751.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA AACATGTTCC TTTTTTGGTC ATAATATCAC TCCTCAGCTT GTCCAAAGAT 
    CACCTGTTCC TGGCGCAGCT TATTCCCGAC CCTGAAGACC TAGAGAGGGG GAACGACAAC
    GGGACGCCAA TCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTCCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CTTCAGACCT AATTGTGTCA
    AAAGGAGAAC CTGCAACTCT GAACTGTAAA GCTGAAGGCC GCCCCACGCC CACCATTGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTGGCACGG AATTACCTCG GAGAGGCTGT GAGCCATAAT
    GCATCGCTGG AGGTGGCTAT ACTACGGGAT GACTTCAGAC AAAATCCTTC TGATGTCATG
    GTGGCAGTGG GGGAACCTGC TGTGATGGAA TGCCAACCCC CACGAGGCCA TCCTGAACCC
    ACCATTTCGT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGTGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCTGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAACGTGAG AGTGAAGTAG CAGAGCTAAC TGTCTTGGAG
    AGACCATCAT TTGTGAAGAG ACCTAGTAAC TTGGCTGTAA CTGTGGATGA CAGTGCAGAA
    TTTAAATGTG AGGCTCGAGG TGACCCTGTG CCTACGGTAC GATGGAGGAA AGACGATGGA
    GAACTGCCCA AATCCAGATA CGAAATCAGA GATGATCATA CTTTGAAGAT TCGGAAAGTG
    ATGGCTGGTG ACATGGGGTC ATATACTTGT GTTGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCAGCTA CTCTGACTGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCT
    CGTGACCAGG TTGTTGCCTT GGGGCGAACT GTAACATTTC AGTGTGAAGC AACCGGAAAT
    CCTCAACCAG CTATTTTTTG GAGAAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCGCCACAGT CAACTAGCCG CTTTTCCGTC TCCCAGACTG GTGATCTCAC TATTACTAAT
    GTCCAGCGGT CTGATGTTGG TTATTATATC TGCCAGACTT TAAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAATGCC CACGATTCTC TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTA GAATCAAACA GCTGGAGACG GGAGTGCTGC AGATCCGATA CGCTAAGCTG
    GGTGACACAG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCACGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCAG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CAACATCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCA
    AATGCCTATG GAATTAGTGA TCCAAGCCAG ATATCAGATC CAGTGAAAAC ACAAGATGTT
    CCCCCAACAA GTCAGGGAGT AGATCACAAG CAGGTCCAGA GAGAATTGGG AAACGTCGTA
    CTGCACCTTC ACAACCCCAC CATCCTCTCA TCCTCTTCCA TCGAAGTGCA CTGGACAGTG
    GACCAACAAT CTCAGTATAT TCAAGGATAT AAAATTCTTT ATCGGCCATC TGGAGGCAAC
    CATGGTGAAT CGGAGTGGTT AGTTTTTGAA GTGAGGACTC CAACCAAAAA TAGCGTGGTT
    ATCCCTGATC TCAAAAAGGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCT TAGAAGAAGC ACCCAGTGCC
    CCACCCCAAA GTGTAACCGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT
    CTGGGCAATG AAACTCGTTA TCACATAAAC AAGACGGTGG ATGGTTCCAC CTTTTCTGTG
    GTCATTCCTT CTCTGGTTCC TGGGATCCGA TACAGCGTAG AAGTAGCAGC CAGCACGGGA
    GCTGGAGCTG GGGTGAAGAG CGAGCCACAG TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AAGACCAAGT CAGCCTTGCT CAGCAGATCT CAGATGTTGT GAAGCAGCCA
    GCGTTCATTG CGGGAATCGG GGCCGCCTGC TGGATCATCC TCATGGTCTT TAGCATCTGG
    CTCTATCGAC ACCGGAAGAA GAGAAATGGA CTGACTAGTA CTTATGCGGG TATCAGAAAA
    GTAACTTATC AGAGAGGAGG TGAAGCTGTG AGCAGTGGAG GGAGGCCAGG ACTTCTGAAC
    ATCAGCGAAC CTGCCACACA GCCCTGGCTG GCAGACACGT GGCCCAATAC TGGCAACAAT
    CACAATGACT GCTCTATCAA TTGCTGTACG GCAGGCAATG GTAACAGTGA TAACAACCTG
    ACCACCTACA GTCGCCCAGC CGATTGTATA GCAAATTATA ACAACCAACT GGATAACAAA
    CAAACAAATC TGATGCTCCC TGAGTCAACT GTTTATGGTG ATGTGGACCT TAGTAACAAA
    ATCAATGAGA TGAAAACCTT CAATAGCCCA AATCTGAAGG ATGGGCGTTT TGTCAATCCA
    TCAGGGCAGC CCACTCCTTA TGCCACCACC CAGCTCATCC AGTCAAACCT CAGCAACAAC
    ATGAACAATG GCAGCGGGGA CTCCAGCGAG AAGCACTGGA AACCTCCCGG CCAGCAGAAA
    CAAGAAGTGC CACCAGTTCA GTATAACATC ATGGAGCAAA ACAAACTGAA CAAAGATTAT
    CGAGCAAATG ACACAGTTCC TCCAACTATC CCATACAACC AATCATATGA CCAGAATACA
    GGAGGATCCT ACAACAGTTC AGACCGGGGC AGTAGTACAT CTGGGAGTCA AGGGCACAAG
    AAAGGGGCAC GGACACCCAA AGTACCAAAA CAAGGTGGCA TGAACTGGGC AGACCTGCTT
    CCTCCTCCCC CAGCGCATCC TCCTCCTCAT AGCAATAGTG AAGAGTACAA TATTTCTGTA
    GATGAAAGCT ATGACCAAGA AATGCCATGT CCCGTGCCAC CAGCAAGGAT GTATTTGCAA
    CAGAATGAAC TAGAAGAGGA GGAAGATGAG CGAGGCCCCA CTCCCCCTGT GCGGGGAGCA
    GCTTCTTCTC CAGCTGCTGT GTCTTATAGC CATCAGTCCA CTGCTACTCT GACCCCCTCC
    CCACAAGAAG AACTCCAGCC CATGTTGCAG GACTGCCCGG AGGAGATTGG CCACATGCAG
    CACCAACCCG ATAGGAGACG GCAGCCTGTG AGTCCTCCTC CACCACCAAG ACCGATTTCC
    CCACCACATA CCTATGGCTA CATTTCAGGA CCTCTTGTCT CAGATATGGA TACAGATGCA
    CCAGAAGAGG AAGAAGATGA AGCAGACATG GAAGTGGCCA AGATGCAGAC CAGACGGCTT
    TTGTTACGTG GCCTTGAGCA GACCCCTGCC TCCAGTGTGG GGGACCTTGA AAGCTCTGTC
    ACTGGGTCCA TGATCAATGG CTGGGGCTCA GCCTCAGAGG AGGACAACAT TTCCAGTGGA
    CGCTCCAGTG TGAGTTCTTC TGATGGCTCC TTCTTCACTG ATGCTGATTT TGCCCAGGCA
    GTGGCAGCAG CTGCAGAATA TGCTGGTCTT AAAGTGGCAC GTCGTCAGAT GCAGGATGCT
    GCTGGTCGCC GACATTTTCA TGCATCCCAG TGCCCTCGAC CCACAAGTCC TGTCTCTACA
    GACAGCAACA TGAGTGCTGC TGTAATGCAG AAGGCCAGAC CAGCCAAGAA GCAAAAACAC
    CAGCCAGGAC ATCTGCGCAG AGAAGTCTAC ACAGATGATC TACCACCTCC TCCGGTGCCA
    CCACCTGCTA TAAAATCACC CACTGTCCAA TCCAAGGCAC AGCTGGAGGT GCGACCTGTT
    ATGGTGCCAA AACTCCCTTC TATAGAAGCA AGAACAGACC GATCATCAGA TAGAAAAGTA
    GGCAGTTACA AGGGGAGAGA AGTACTGGAT GGAAGACAGG TTGCTGATAT GAGAACCAAT
    CCAGGTGATA CCAGAGAAGC ACAGGAACAG CAAAATGATG GAAAAGGACG AGTAAACAAG
    GCAGCAAAAC GAGACCTTCC ACCAGCAAAG ACTCACCTCA TCCAAGAGGA TATCCTACCT
    TACTGTAGAC CTACTTTTCC GACATCAAAT AATCCCAGAG ATCCTAGTTC TTCAAGCTCA
    ATGTCATCAA GAGGATCAGG AAGCAGACAA AGAGAACAAG CAAATGTAGG TCGAAGAAAT
    ATCGCAGAAA TGCAAGTTCT TGGAGGATAT GAAAGAGGAG AAGATAATAA TGAAGAATTA
    GAGGAAACTG AAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44465
    Clone ID Related Accession (Same CDS sequence) XM_014109752.1
    Accession Version XM_014109752.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product roundabout homolog 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA AACATGTTCC TTTTTTGGTC ATAATATCAC TCCTCAGCTT GTCCAAAGAT 
    CACCTGTTCC TGGCGCAGCT TATTCCCGAC CCTGAAGACC TAGAGAGGGG GAACGACAAC
    GGGACGCCAA TCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTCCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CTTCAGACCT AATTGTGTCA
    AAAGGAGAAC CTGCAACTCT GAACTGTAAA GCTGAAGGCC GCCCCACGCC CACCATTGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTGGCACGG AATTACCTCG GAGAGGCTGT GAGCCATAAT
    GCATCGCTGG AGGTGGCTAT ACTACGGGAT GACTTCAGAC AAAATCCTTC TGATGTCATG
    GTGGCAGTGG GGGAACCTGC TGTGATGGAA TGCCAACCCC CACGAGGCCA TCCTGAACCC
    ACCATTTCGT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGTGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCTGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAACGTGAG AGTGAAGTAG CAGAGCTAAC TGTCTTGGAG
    AGACCATCAT TTGTGAAGAG ACCTAGTAAC TTGGCTGTAA CTGTGGATGA CAGTGCAGAA
    TTTAAATGTG AGGCTCGAGG TGACCCTGTG CCTACGGTAC GATGGAGGAA AGACGATGGA
    GAACTGCCCA AATCCAGATA CGAAATCAGA GATGATCATA CTTTGAAGAT TCGGAAAGTG
    ATGGCTGGTG ACATGGGGTC ATATACTTGT GTTGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCAGCTA CTCTGACTGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCT
    CGTGACCAGG TTGTTGCCTT GGGGCGAACT GTAACATTTC AGTGTGAAGC AACCGGAAAT
    CCTCAACCAG CTATTTTTTG GAGAAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCGCCACAGT CAACTAGCCG CTTTTCCGTC TCCCAGACTG GTGATCTCAC TATTACTAAT
    GTCCAGCGGT CTGATGTTGG TTATTATATC TGCCAGACTT TAAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAATGCC CACGATTCTC TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTA GAATCAAACA GCTGGAGACG GGAGTGCTGC AGATCCGATA CGCTAAGCTG
    GGTGACACAG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCACGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCAG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CAACATCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCA
    AATGCCTATG GAATTAGTGA TCCAAGCCAG ATATCAGATC CAGTGAAAAC ACAAGATGTT
    CCCCCAACAA GTCAGGGAGT AGATCACAAG CAGGTCCAGA GAGAATTGGG AAACGTCGTA
    CTGCACCTTC ACAACCCCAC CATCCTCTCA TCCTCTTCCA TCGAAGTGCA CTGGACAGTG
    GACCAACAAT CTCAGTATAT TCAAGGATAT AAAATTCTTT ATCGGCCATC TGGAGGCAAC
    CATGGTGAAT CGGAGTGGTT AGTTTTTGAA GTGAGGACTC CAACCAAAAA TAGCGTGGTT
    ATCCCTGATC TCAAAAAGGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCT TAGAAGAAGC ACCCAGTGCC
    CCACCCCAAA GTGTAACCGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT
    CTGGGCAATG AAACTCGTTA TCACATAAAC AAGACGGTGG ATGGTTCCAC CTTTTCTGTG
    GTCATTCCTT CTCTGGTTCC TGGGATCCGA TACAGCGTAG AAGTAGCAGC CAGCACGGGA
    GCTGGAGCTG GGGTGAAGAG CGAGCCACAG TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AAGACCAAGT CAGCCTTGCT CAGCAGATCT CAGATGTTGT GAAGCAGCCA
    GCGTTCATTG CGGGAATCGG GGCCGCCTGC TGGATCATCC TCATGGTCTT TAGCATCTGG
    CTCTATCGAC ACCGGAAGAA GAGAAATGGA CTGACTAGTA CTTATGCGGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGTGA AGCTGTGAGC
    AGTGGAGGGA GGCCAGGACT TCTGAACATC AGCGAACCTG CCACACAGCC CTGGCTGGCA
    GACACGTGGC CCAATACTGG CAACAATCAC AATGACTGCT CTATCAATTG CTGTACGGCA
    GGCAATGCCG ATTGTATAGC AAATTATAAC AACCAACTGG ATAACAAACA AACAAATCTG
    ATGCTCCCTG AGTCAACTGT TTATGGTGAT GTGGACCTTA GTAACAAAAT CAATGAGATG
    AAAACCTTCA ATAGCCCAAA TCTGAAGGAT GGGCGTTTTG TCAATCCATC AGGGCAGCCC
    ACTCCTTATG CCACCACCCA GCTCATCCAG TCAAACCTCA GCAACAACAT GAACAATGGC
    AGCGGGGACT CCAGCGAGAA GCACTGGAAA CCTCCCGGCC AGCAGAAACA AGAAGTGCCA
    CCAGTTCAGT ATAACATCAT GGAGCAAAAC AAACTGAACA AAGATTATCG AGCAAATGAC
    ACAGTTCCTC CAACTATCCC ATACAACCAA TCATATGACC AGAATACAGG AGGATCCTAC
    AACAGTTCAG ACCGGGGCAG TAGTACATCT GGGAGTCAAG GGCACAAGAA AGGGGCACGG
    ACACCCAAAG TACCAAAACA AGGTGGCATG AACTGGGCAG ACCTGCTTCC TCCTCCCCCA
    GCGCATCCTC CTCCTCATAG CAATAGTGAA GAGTACAATA TTTCTGTAGA TGAAAGCTAT
    GACCAAGAAA TGCCATGTCC CGTGCCACCA GCAAGGATGT ATTTGCAACA GAATGAACTA
    GAAGAGGAGG AAGATGAGCG AGGCCCCACT CCCCCTGTGC GGGGAGCAGC TTCTTCTCCA
    GCTGCTGTGT CTTATAGCCA TCAGTCCACT GCTACTCTGA CCCCCTCCCC ACAAGAAGAA
    CTCCAGCCCA TGTTGCAGGA CTGCCCGGAG GAGATTGGCC ACATGCAGCA CCAACCCGAT
    AGGAGACGGC AGCCTGTGAG TCCTCCTCCA CCACCAAGAC CGATTTCCCC ACCACATACC
    TATGGCTACA TTTCAGGACC TCTTGTCTCA GATATGGATA CAGATGCACC AGAAGAGGAA
    GAAGATGAAG CAGACATGGA AGTGGCCAAG ATGCAGACCA GACGGCTTTT GTTACGTGGC
    CTTGAGCAGA CCCCTGCCTC CAGTGTGGGG GACCTTGAAA GCTCTGTCAC TGGGTCCATG
    ATCAATGGCT GGGGCTCAGC CTCAGAGGAG GACAACATTT CCAGTGGACG CTCCAGTGTG
    AGTTCTTCTG ATGGCTCCTT CTTCACTGAT GCTGATTTTG CCCAGGCAGT GGCAGCAGCT
    GCAGAATATG CTGGTCTTAA AGTGGCACGT CGTCAGATGC AGGATGCTGC TGGTCGCCGA
    CATTTTCATG CATCCCAGTG CCCTCGACCC ACAAGTCCTG TCTCTACAGA CAGCAACATG
    AGTGCTGCTG TAATGCAGAA GGCCAGACCA GCCAAGAAGC AAAAACACCA GCCAGGACAT
    CTGCGCAGAG AAGTCTACAC AGATGATCTA CCACCTCCTC CGGTGCCACC ACCTGCTATA
    AAATCACCCA CTGTCCAATC CAAGGCACAG CTGGAGGTGC GACCTGTTAT GGTGCCAAAA
    CTCCCTTCTA TAGAAGCAAG AACAGACCGA TCATCAGATA GAAAAGTAGG CAGTTACAAG
    GGGAGAGAAG TACTGGATGG AAGACAGGTT GCTGATATGA GAACCAATCC AGGTGATACC
    AGAGAAGCAC AGGAACAGCA AAATGATGGA AAAGGACGAG TAAACAAGGC AGCAAAACGA
    GACCTTCCAC CAGCAAAGAC TCACCTCATC CAAGAGGATA TCCTACCTTA CTGTAGACCT
    ACTTTTCCGA CATCAAATAA TCCCAGAGAT CCTAGTTCTT CAAGCTCAAT GTCATCAAGA
    GGATCAGGAA GCAGACAAAG AGAACAAGCA AATGTAGGTC GAAGAAATAT CGCAGAAATG
    CAAGTTCTTG GAGGATATGA AAGAGGAGAA GATAATAATG AAGAATTAGA GGAAACTGAA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965227.1
    CDS304..5229
    Translation

    Target ORF information:

    RefSeq Version XM_014109752.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109752.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA AACATGTTCC TTTTTTGGTC ATAATATCAC TCCTCAGCTT GTCCAAAGAT 
    CACCTGTTCC TGGCGCAGCT TATTCCCGAC CCTGAAGACC TAGAGAGGGG GAACGACAAC
    GGGACGCCAA TCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTCCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CTTCAGACCT AATTGTGTCA
    AAAGGAGAAC CTGCAACTCT GAACTGTAAA GCTGAAGGCC GCCCCACGCC CACCATTGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTGGCACGG AATTACCTCG GAGAGGCTGT GAGCCATAAT
    GCATCGCTGG AGGTGGCTAT ACTACGGGAT GACTTCAGAC AAAATCCTTC TGATGTCATG
    GTGGCAGTGG GGGAACCTGC TGTGATGGAA TGCCAACCCC CACGAGGCCA TCCTGAACCC
    ACCATTTCGT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGTGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCTGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAACGTGAG AGTGAAGTAG CAGAGCTAAC TGTCTTGGAG
    AGACCATCAT TTGTGAAGAG ACCTAGTAAC TTGGCTGTAA CTGTGGATGA CAGTGCAGAA
    TTTAAATGTG AGGCTCGAGG TGACCCTGTG CCTACGGTAC GATGGAGGAA AGACGATGGA
    GAACTGCCCA AATCCAGATA CGAAATCAGA GATGATCATA CTTTGAAGAT TCGGAAAGTG
    ATGGCTGGTG ACATGGGGTC ATATACTTGT GTTGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCAGCTA CTCTGACTGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCT
    CGTGACCAGG TTGTTGCCTT GGGGCGAACT GTAACATTTC AGTGTGAAGC AACCGGAAAT
    CCTCAACCAG CTATTTTTTG GAGAAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCGCCACAGT CAACTAGCCG CTTTTCCGTC TCCCAGACTG GTGATCTCAC TATTACTAAT
    GTCCAGCGGT CTGATGTTGG TTATTATATC TGCCAGACTT TAAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAATGCC CACGATTCTC TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTA GAATCAAACA GCTGGAGACG GGAGTGCTGC AGATCCGATA CGCTAAGCTG
    GGTGACACAG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCACGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCAG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CAACATCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCA
    AATGCCTATG GAATTAGTGA TCCAAGCCAG ATATCAGATC CAGTGAAAAC ACAAGATGTT
    CCCCCAACAA GTCAGGGAGT AGATCACAAG CAGGTCCAGA GAGAATTGGG AAACGTCGTA
    CTGCACCTTC ACAACCCCAC CATCCTCTCA TCCTCTTCCA TCGAAGTGCA CTGGACAGTG
    GACCAACAAT CTCAGTATAT TCAAGGATAT AAAATTCTTT ATCGGCCATC TGGAGGCAAC
    CATGGTGAAT CGGAGTGGTT AGTTTTTGAA GTGAGGACTC CAACCAAAAA TAGCGTGGTT
    ATCCCTGATC TCAAAAAGGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCT TAGAAGAAGC ACCCAGTGCC
    CCACCCCAAA GTGTAACCGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT
    CTGGGCAATG AAACTCGTTA TCACATAAAC AAGACGGTGG ATGGTTCCAC CTTTTCTGTG
    GTCATTCCTT CTCTGGTTCC TGGGATCCGA TACAGCGTAG AAGTAGCAGC CAGCACGGGA
    GCTGGAGCTG GGGTGAAGAG CGAGCCACAG TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AAGACCAAGT CAGCCTTGCT CAGCAGATCT CAGATGTTGT GAAGCAGCCA
    GCGTTCATTG CGGGAATCGG GGCCGCCTGC TGGATCATCC TCATGGTCTT TAGCATCTGG
    CTCTATCGAC ACCGGAAGAA GAGAAATGGA CTGACTAGTA CTTATGCGGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGTGA AGCTGTGAGC
    AGTGGAGGGA GGCCAGGACT TCTGAACATC AGCGAACCTG CCACACAGCC CTGGCTGGCA
    GACACGTGGC CCAATACTGG CAACAATCAC AATGACTGCT CTATCAATTG CTGTACGGCA
    GGCAATGCCG ATTGTATAGC AAATTATAAC AACCAACTGG ATAACAAACA AACAAATCTG
    ATGCTCCCTG AGTCAACTGT TTATGGTGAT GTGGACCTTA GTAACAAAAT CAATGAGATG
    AAAACCTTCA ATAGCCCAAA TCTGAAGGAT GGGCGTTTTG TCAATCCATC AGGGCAGCCC
    ACTCCTTATG CCACCACCCA GCTCATCCAG TCAAACCTCA GCAACAACAT GAACAATGGC
    AGCGGGGACT CCAGCGAGAA GCACTGGAAA CCTCCCGGCC AGCAGAAACA AGAAGTGCCA
    CCAGTTCAGT ATAACATCAT GGAGCAAAAC AAACTGAACA AAGATTATCG AGCAAATGAC
    ACAGTTCCTC CAACTATCCC ATACAACCAA TCATATGACC AGAATACAGG AGGATCCTAC
    AACAGTTCAG ACCGGGGCAG TAGTACATCT GGGAGTCAAG GGCACAAGAA AGGGGCACGG
    ACACCCAAAG TACCAAAACA AGGTGGCATG AACTGGGCAG ACCTGCTTCC TCCTCCCCCA
    GCGCATCCTC CTCCTCATAG CAATAGTGAA GAGTACAATA TTTCTGTAGA TGAAAGCTAT
    GACCAAGAAA TGCCATGTCC CGTGCCACCA GCAAGGATGT ATTTGCAACA GAATGAACTA
    GAAGAGGAGG AAGATGAGCG AGGCCCCACT CCCCCTGTGC GGGGAGCAGC TTCTTCTCCA
    GCTGCTGTGT CTTATAGCCA TCAGTCCACT GCTACTCTGA CCCCCTCCCC ACAAGAAGAA
    CTCCAGCCCA TGTTGCAGGA CTGCCCGGAG GAGATTGGCC ACATGCAGCA CCAACCCGAT
    AGGAGACGGC AGCCTGTGAG TCCTCCTCCA CCACCAAGAC CGATTTCCCC ACCACATACC
    TATGGCTACA TTTCAGGACC TCTTGTCTCA GATATGGATA CAGATGCACC AGAAGAGGAA
    GAAGATGAAG CAGACATGGA AGTGGCCAAG ATGCAGACCA GACGGCTTTT GTTACGTGGC
    CTTGAGCAGA CCCCTGCCTC CAGTGTGGGG GACCTTGAAA GCTCTGTCAC TGGGTCCATG
    ATCAATGGCT GGGGCTCAGC CTCAGAGGAG GACAACATTT CCAGTGGACG CTCCAGTGTG
    AGTTCTTCTG ATGGCTCCTT CTTCACTGAT GCTGATTTTG CCCAGGCAGT GGCAGCAGCT
    GCAGAATATG CTGGTCTTAA AGTGGCACGT CGTCAGATGC AGGATGCTGC TGGTCGCCGA
    CATTTTCATG CATCCCAGTG CCCTCGACCC ACAAGTCCTG TCTCTACAGA CAGCAACATG
    AGTGCTGCTG TAATGCAGAA GGCCAGACCA GCCAAGAAGC AAAAACACCA GCCAGGACAT
    CTGCGCAGAG AAGTCTACAC AGATGATCTA CCACCTCCTC CGGTGCCACC ACCTGCTATA
    AAATCACCCA CTGTCCAATC CAAGGCACAG CTGGAGGTGC GACCTGTTAT GGTGCCAAAA
    CTCCCTTCTA TAGAAGCAAG AACAGACCGA TCATCAGATA GAAAAGTAGG CAGTTACAAG
    GGGAGAGAAG TACTGGATGG AAGACAGGTT GCTGATATGA GAACCAATCC AGGTGATACC
    AGAGAAGCAC AGGAACAGCA AAATGATGGA AAAGGACGAG TAAACAAGGC AGCAAAACGA
    GACCTTCCAC CAGCAAAGAC TCACCTCATC CAAGAGGATA TCCTACCTTA CTGTAGACCT
    ACTTTTCCGA CATCAAATAA TCCCAGAGAT CCTAGTTCTT CAAGCTCAAT GTCATCAAGA
    GGATCAGGAA GCAGACAAAG AGAACAAGCA AATGTAGGTC GAAGAAATAT CGCAGAAATG
    CAAGTTCTTG GAGGATATGA AAGAGGAGAA GATAATAATG AAGAATTAGA GGAAACTGAA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44466
    Clone ID Related Accession (Same CDS sequence) XM_014109753.1
    Accession Version XM_014109753.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4902bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product roundabout homolog 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTGTGC TGTTACTGAT GCTGCTGCTC TCGATGGCAG AAATAACTTC TCCTGTGGGT 
    GTCTCGGGCA GAATGAAGGC TGGGTCAAGG TGGAGAGGTG AAAAGAATCG CTCCCGTCTC
    CGTCAGGAAG ATTTTCCACC TCGTATTGTT GAACACCCTT CAGACCTAAT TGTGTCAAAA
    GGAGAACCTG CAACTCTGAA CTGTAAAGCT GAAGGCCGCC CCACGCCCAC CATTGAATGG
    TACAAAGGTG GGGAGAGAGT GGAGACAGAC AAAGATGACC CTCGCTCACA CCGAATGTTG
    CTGCCAAGTG GATCTTTATT TTTCTTACGT ATAGTACATG GACGAAAAAG TAGGCCTGAT
    GAAGGGGTCT ACGTCTGTGT GGCACGGAAT TACCTCGGAG AGGCTGTGAG CCATAATGCA
    TCGCTGGAGG TGGCTATACT ACGGGATGAC TTCAGACAAA ATCCTTCTGA TGTCATGGTG
    GCAGTGGGGG AACCTGCTGT GATGGAATGC CAACCCCCAC GAGGCCATCC TGAACCCACC
    ATTTCGTGGA AGAAAGATGG CTCTCCACTG GATGATAAAG ATGAAAGAAT AACTATACGA
    GGTGGAAAGC TCATGATTAC TTACACCCGC AAAAGTGATG CTGGCAAATA TGTTTGTGTT
    GGTACCAACA TGGTTGGGGA ACGTGAGAGT GAAGTAGCAG AGCTAACTGT CTTGGAGAGA
    CCATCATTTG TGAAGAGACC TAGTAACTTG GCTGTAACTG TGGATGACAG TGCAGAATTT
    AAATGTGAGG CTCGAGGTGA CCCTGTGCCT ACGGTACGAT GGAGGAAAGA CGATGGAGAA
    CTGCCCAAAT CCAGATACGA AATCAGAGAT GATCATACTT TGAAGATTCG GAAAGTGATG
    GCTGGTGACA TGGGGTCATA TACTTGTGTT GCAGAAAATA TGGTTGGAAA AGCTGAAGCA
    TCAGCTACTC TGACTGTCCA AGTTGGGTCT GAACCTCCAC ATTTTGTTGT GAAACCTCGT
    GACCAGGTTG TTGCCTTGGG GCGAACTGTA ACATTTCAGT GTGAAGCAAC CGGAAATCCT
    CAACCAGCTA TTTTTTGGAG AAGAGAGGGG AGTCAGAATC TGCTTTTCTC ATATCAGCCG
    CCACAGTCAA CTAGCCGCTT TTCCGTCTCC CAGACTGGTG ATCTCACTAT TACTAATGTC
    CAGCGGTCTG ATGTTGGTTA TTATATCTGC CAGACTTTAA ATGTTGCCGG AAGTATCATC
    ACAAAAGCAT ATTTGGAAGT TACTGATGTG ATTGCAGACC GGCCTCCCCC AGTCATTCGA
    CAAGGTCCTG TGAATCAGAC TGTAGCAGTG GATGGTACTT TAGTGCTCAG CTGTGTGGCC
    ACAGGCAGTC CAATGCCCAC GATTCTCTGG AGAAAGGATG GAGTCCTTGT TTCAACCCAA
    GATTCTAGAA TCAAACAGCT GGAGACGGGA GTGCTGCAGA TCCGATACGC TAAGCTGGGT
    GACACAGGTC GGTACACCTG CATTGCATCA ACCCCCAGTG GTGAAGCAAC ATGGAGTGCT
    TACATTGAAG TCCAAGAATT TGGAGTTCCA GTTCAGCCTC CACGACCCAC CGACCCAAAT
    TTAATCCCTA GTGCCCCCTC AAAACCTGAA GTTACAGATG TCAGCAGAAA TACAGTAACG
    TTGTCATGGC AACCAAATTT GAATTCAGGT GCAACTCCAA CATCTTATAT TATAGAAGCC
    TTCAGCCATG CATCTGGTAG CAGCTGGCAG ACCGTAGCAG AGAATGTGAA AACAGAAACA
    TTTGCCATTA AAGGACTCAA ACCTAATGCA ATTTACCTTT TCCTTGTGAG GGCAGCAAAT
    GCCTATGGAA TTAGTGATCC AAGCCAGATA TCAGATCCAG TGAAAACACA AGATGTTCCC
    CCAACAAGTC AGGGAGTAGA TCACAAGCAG GTCCAGAGAG AATTGGGAAA CGTCGTACTG
    CACCTTCACA ACCCCACCAT CCTCTCATCC TCTTCCATCG AAGTGCACTG GACAGTGGAC
    CAACAATCTC AGTATATTCA AGGATATAAA ATTCTTTATC GGCCATCTGG AGGCAACCAT
    GGTGAATCGG AGTGGTTAGT TTTTGAAGTG AGGACTCCAA CCAAAAATAG CGTGGTTATC
    CCTGATCTCA AAAAGGGAGT CAACTATGAA ATTAAGGCTC GTCCTTTTTT TAATGAATTT
    CAAGGAGCAG ATAGTGAAAT TAAATTTGCC AAAACCTTAG AAGAAGCACC CAGTGCCCCA
    CCCCAAAGTG TAACCGTATC AAAGAATGAT GGAAATGGAA CTGCAATTCT TGTTAGTTGG
    CAGCCACCTC CAGAAGACAC TCAAAATGGA ATGGTCCAAG AGTATAAGGT TTGGTGTCTG
    GGCAATGAAA CTCGTTATCA CATAAACAAG ACGGTGGATG GTTCCACCTT TTCTGTGGTC
    ATTCCTTCTC TGGTTCCTGG GATCCGATAC AGCGTAGAAG TAGCAGCCAG CACGGGAGCT
    GGAGCTGGGG TGAAGAGCGA GCCACAGTTC ATCCAGTTAG ATTCTCATGG AAACCCCGTG
    TCACCTGAAG ACCAAGTCAG CCTTGCTCAG CAGATCTCAG ATGTTGTGAA GCAGCCAGCG
    TTCATTGCGG GAATCGGGGC CGCCTGCTGG ATCATCCTCA TGGTCTTTAG CATCTGGCTC
    TATCGACACC GGAAGAAGAG AAATGGACTG ACTAGTACTT ATGCGGGTAT CAGAAAAGTC
    CCGTCTTTTA CCTTCACACC AACAGTAACT TATCAGAGAG GAGGTGAAGC TGTGAGCAGT
    GGAGGGAGGC CAGGACTTCT GAACATCAGC GAACCTGCCA CACAGCCCTG GCTGGCAGAC
    ACGTGGCCCA ATACTGGCAA CAATCACAAT GACTGCTCTA TCAATTGCTG TACGGCAGGC
    AATGGTAACA GTGATAACAA CCTGACCACC TACAGTCGCC CAGCCGATTG TATAGCAAAT
    TATAACAACC AACTGGATAA CAAACAAACA AATCTGATGC TCCCTGAGTC AACTGTTTAT
    GGTGATGTGG ACCTTAGTAA CAAAATCAAT GAGATGAAAA CCTTCAATAG CCCAAATCTG
    AAGGATGGGC GTTTTGTCAA TCCATCAGGG CAGCCCACTC CTTATGCCAC CACCCAGCTC
    ATCCAGTCAA ACCTCAGCAA CAACATGAAC AATGGCAGCG GGGACTCCAG CGAGAAGCAC
    TGGAAACCTC CCGGCCAGCA GAAACAAGAA GTGCCACCAG TTCAGTATAA CATCATGGAG
    CAAAACAAAC TGAACAAAGA TTATCGAGCA AATGACACAG TTCCTCCAAC TATCCCATAC
    AACCAATCAT ATGACCAGAA TACAGGAGGA TCCTACAACA GTTCAGACCG GGGCAGTAGT
    ACATCTGGGA GTCAAGGGCA CAAGAAAGGG GCACGGACAC CCAAAGTACC AAAACAAGGT
    GGCATGAACT GGGCAGACCT GCTTCCTCCT CCCCCAGCGC ATCCTCCTCC TCATAGCAAT
    AGTGAAGAGT ACAATATTTC TGTAGATGAA AGCTATGACC AAGAAATGCC ATGTCCCGTG
    CCACCAGCAA GGATGTATTT GCAACAGAAT GAACTAGAAG AGGAGGAAGA TGAGCGAGGC
    CCCACTCCCC CTGTGCGGGG AGCAGCTTCT TCTCCAGCTG CTGTGTCTTA TAGCCATCAG
    TCCACTGCTA CTCTGACCCC CTCCCCACAA GAAGAACTCC AGCCCATGTT GCAGGACTGC
    CCGGAGGAGA TTGGCCACAT GCAGCACCAA CCCGATAGGA GACGGCAGCC TGTGAGTCCT
    CCTCCACCAC CAAGACCGAT TTCCCCACCA CATACCTATG GCTACATTTC AGGACCTCTT
    GTCTCAGATA TGGATACAGA TGCACCAGAA GAGGAAGAAG ATGAAGCAGA CATGGAAGTG
    GCCAAGATGC AGACCAGACG GCTTTTGTTA CGTGGCCTTG AGCAGACCCC TGCCTCCAGT
    GTGGGGGACC TTGAAAGCTC TGTCACTGGG TCCATGATCA ATGGCTGGGG CTCAGCCTCA
    GAGGAGGACA ACATTTCCAG TGGACGCTCC AGTGTGAGTT CTTCTGATGG CTCCTTCTTC
    ACTGATGCTG ATTTTGCCCA GGCAGTGGCA GCAGCTGCAG AATATGCTGG TCTTAAAGTG
    GCACGTCGTC AGATGCAGGA TGCTGCTGGT CGCCGACATT TTCATGCATC CCAGTGCCCT
    CGACCCACAA GTCCTGTCTC TACAGACAGC AACATGAGTG CTGCTGTAAT GCAGAAGGCC
    AGACCAGCCA AGAAGCAAAA ACACCAGCCA GGACATCTGC GCAGAGAAGT CTACACAGAT
    GATCTACCAC CTCCTCCGGT GCCACCACCT GCTATAAAAT CACCCACTGT CCAATCCAAG
    GCACAGCTGG AGGTGCGACC TGTTATGGTG CCAAAACTCC CTTCTATAGA AGCAAGAACA
    GACCGATCAT CAGATAGAAA AGTAGGCAGT TACAAGGGGA GAGAAGTACT GGATGGAAGA
    CAGGTTGCTG ATATGAGAAC CAATCCAGGT GATACCAGAG AAGCACAGGA ACAGCAAAAT
    GATGGAAAAG GACGAGTAAA CAAGGCAGCA AAACGAGACC TTCCACCAGC AAAGACTCAC
    CTCATCCAAG AGGATATCCT ACCTTACTGT AGACCTACTT TTCCGACATC AAATAATCCC
    AGAGATCCTA GTTCTTCAAG CTCAATGTCA TCAAGAGGAT CAGGAAGCAG ACAAAGAGAA
    CAAGCAAATG TAGGTCGAAG AAATATCGCA GAAATGCAAG TTCTTGGAGG ATATGAAAGA
    GGAGAAGATA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965228.1
    CDS16..4917
    Translation

    Target ORF information:

    RefSeq Version XM_014109753.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109753.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTGTGC TGTTACTGAT GCTGCTGCTC TCGATGGCAG AAATAACTTC TCCTGTGGGT 
    GTCTCGGGCA GAATGAAGGC TGGGTCAAGG TGGAGAGGTG AAAAGAATCG CTCCCGTCTC
    CGTCAGGAAG ATTTTCCACC TCGTATTGTT GAACACCCTT CAGACCTAAT TGTGTCAAAA
    GGAGAACCTG CAACTCTGAA CTGTAAAGCT GAAGGCCGCC CCACGCCCAC CATTGAATGG
    TACAAAGGTG GGGAGAGAGT GGAGACAGAC AAAGATGACC CTCGCTCACA CCGAATGTTG
    CTGCCAAGTG GATCTTTATT TTTCTTACGT ATAGTACATG GACGAAAAAG TAGGCCTGAT
    GAAGGGGTCT ACGTCTGTGT GGCACGGAAT TACCTCGGAG AGGCTGTGAG CCATAATGCA
    TCGCTGGAGG TGGCTATACT ACGGGATGAC TTCAGACAAA ATCCTTCTGA TGTCATGGTG
    GCAGTGGGGG AACCTGCTGT GATGGAATGC CAACCCCCAC GAGGCCATCC TGAACCCACC
    ATTTCGTGGA AGAAAGATGG CTCTCCACTG GATGATAAAG ATGAAAGAAT AACTATACGA
    GGTGGAAAGC TCATGATTAC TTACACCCGC AAAAGTGATG CTGGCAAATA TGTTTGTGTT
    GGTACCAACA TGGTTGGGGA ACGTGAGAGT GAAGTAGCAG AGCTAACTGT CTTGGAGAGA
    CCATCATTTG TGAAGAGACC TAGTAACTTG GCTGTAACTG TGGATGACAG TGCAGAATTT
    AAATGTGAGG CTCGAGGTGA CCCTGTGCCT ACGGTACGAT GGAGGAAAGA CGATGGAGAA
    CTGCCCAAAT CCAGATACGA AATCAGAGAT GATCATACTT TGAAGATTCG GAAAGTGATG
    GCTGGTGACA TGGGGTCATA TACTTGTGTT GCAGAAAATA TGGTTGGAAA AGCTGAAGCA
    TCAGCTACTC TGACTGTCCA AGTTGGGTCT GAACCTCCAC ATTTTGTTGT GAAACCTCGT
    GACCAGGTTG TTGCCTTGGG GCGAACTGTA ACATTTCAGT GTGAAGCAAC CGGAAATCCT
    CAACCAGCTA TTTTTTGGAG AAGAGAGGGG AGTCAGAATC TGCTTTTCTC ATATCAGCCG
    CCACAGTCAA CTAGCCGCTT TTCCGTCTCC CAGACTGGTG ATCTCACTAT TACTAATGTC
    CAGCGGTCTG ATGTTGGTTA TTATATCTGC CAGACTTTAA ATGTTGCCGG AAGTATCATC
    ACAAAAGCAT ATTTGGAAGT TACTGATGTG ATTGCAGACC GGCCTCCCCC AGTCATTCGA
    CAAGGTCCTG TGAATCAGAC TGTAGCAGTG GATGGTACTT TAGTGCTCAG CTGTGTGGCC
    ACAGGCAGTC CAATGCCCAC GATTCTCTGG AGAAAGGATG GAGTCCTTGT TTCAACCCAA
    GATTCTAGAA TCAAACAGCT GGAGACGGGA GTGCTGCAGA TCCGATACGC TAAGCTGGGT
    GACACAGGTC GGTACACCTG CATTGCATCA ACCCCCAGTG GTGAAGCAAC ATGGAGTGCT
    TACATTGAAG TCCAAGAATT TGGAGTTCCA GTTCAGCCTC CACGACCCAC CGACCCAAAT
    TTAATCCCTA GTGCCCCCTC AAAACCTGAA GTTACAGATG TCAGCAGAAA TACAGTAACG
    TTGTCATGGC AACCAAATTT GAATTCAGGT GCAACTCCAA CATCTTATAT TATAGAAGCC
    TTCAGCCATG CATCTGGTAG CAGCTGGCAG ACCGTAGCAG AGAATGTGAA AACAGAAACA
    TTTGCCATTA AAGGACTCAA ACCTAATGCA ATTTACCTTT TCCTTGTGAG GGCAGCAAAT
    GCCTATGGAA TTAGTGATCC AAGCCAGATA TCAGATCCAG TGAAAACACA AGATGTTCCC
    CCAACAAGTC AGGGAGTAGA TCACAAGCAG GTCCAGAGAG AATTGGGAAA CGTCGTACTG
    CACCTTCACA ACCCCACCAT CCTCTCATCC TCTTCCATCG AAGTGCACTG GACAGTGGAC
    CAACAATCTC AGTATATTCA AGGATATAAA ATTCTTTATC GGCCATCTGG AGGCAACCAT
    GGTGAATCGG AGTGGTTAGT TTTTGAAGTG AGGACTCCAA CCAAAAATAG CGTGGTTATC
    CCTGATCTCA AAAAGGGAGT CAACTATGAA ATTAAGGCTC GTCCTTTTTT TAATGAATTT
    CAAGGAGCAG ATAGTGAAAT TAAATTTGCC AAAACCTTAG AAGAAGCACC CAGTGCCCCA
    CCCCAAAGTG TAACCGTATC AAAGAATGAT GGAAATGGAA CTGCAATTCT TGTTAGTTGG
    CAGCCACCTC CAGAAGACAC TCAAAATGGA ATGGTCCAAG AGTATAAGGT TTGGTGTCTG
    GGCAATGAAA CTCGTTATCA CATAAACAAG ACGGTGGATG GTTCCACCTT TTCTGTGGTC
    ATTCCTTCTC TGGTTCCTGG GATCCGATAC AGCGTAGAAG TAGCAGCCAG CACGGGAGCT
    GGAGCTGGGG TGAAGAGCGA GCCACAGTTC ATCCAGTTAG ATTCTCATGG AAACCCCGTG
    TCACCTGAAG ACCAAGTCAG CCTTGCTCAG CAGATCTCAG ATGTTGTGAA GCAGCCAGCG
    TTCATTGCGG GAATCGGGGC CGCCTGCTGG ATCATCCTCA TGGTCTTTAG CATCTGGCTC
    TATCGACACC GGAAGAAGAG AAATGGACTG ACTAGTACTT ATGCGGGTAT CAGAAAAGTC
    CCGTCTTTTA CCTTCACACC AACAGTAACT TATCAGAGAG GAGGTGAAGC TGTGAGCAGT
    GGAGGGAGGC CAGGACTTCT GAACATCAGC GAACCTGCCA CACAGCCCTG GCTGGCAGAC
    ACGTGGCCCA ATACTGGCAA CAATCACAAT GACTGCTCTA TCAATTGCTG TACGGCAGGC
    AATGGTAACA GTGATAACAA CCTGACCACC TACAGTCGCC CAGCCGATTG TATAGCAAAT
    TATAACAACC AACTGGATAA CAAACAAACA AATCTGATGC TCCCTGAGTC AACTGTTTAT
    GGTGATGTGG ACCTTAGTAA CAAAATCAAT GAGATGAAAA CCTTCAATAG CCCAAATCTG
    AAGGATGGGC GTTTTGTCAA TCCATCAGGG CAGCCCACTC CTTATGCCAC CACCCAGCTC
    ATCCAGTCAA ACCTCAGCAA CAACATGAAC AATGGCAGCG GGGACTCCAG CGAGAAGCAC
    TGGAAACCTC CCGGCCAGCA GAAACAAGAA GTGCCACCAG TTCAGTATAA CATCATGGAG
    CAAAACAAAC TGAACAAAGA TTATCGAGCA AATGACACAG TTCCTCCAAC TATCCCATAC
    AACCAATCAT ATGACCAGAA TACAGGAGGA TCCTACAACA GTTCAGACCG GGGCAGTAGT
    ACATCTGGGA GTCAAGGGCA CAAGAAAGGG GCACGGACAC CCAAAGTACC AAAACAAGGT
    GGCATGAACT GGGCAGACCT GCTTCCTCCT CCCCCAGCGC ATCCTCCTCC TCATAGCAAT
    AGTGAAGAGT ACAATATTTC TGTAGATGAA AGCTATGACC AAGAAATGCC ATGTCCCGTG
    CCACCAGCAA GGATGTATTT GCAACAGAAT GAACTAGAAG AGGAGGAAGA TGAGCGAGGC
    CCCACTCCCC CTGTGCGGGG AGCAGCTTCT TCTCCAGCTG CTGTGTCTTA TAGCCATCAG
    TCCACTGCTA CTCTGACCCC CTCCCCACAA GAAGAACTCC AGCCCATGTT GCAGGACTGC
    CCGGAGGAGA TTGGCCACAT GCAGCACCAA CCCGATAGGA GACGGCAGCC TGTGAGTCCT
    CCTCCACCAC CAAGACCGAT TTCCCCACCA CATACCTATG GCTACATTTC AGGACCTCTT
    GTCTCAGATA TGGATACAGA TGCACCAGAA GAGGAAGAAG ATGAAGCAGA CATGGAAGTG
    GCCAAGATGC AGACCAGACG GCTTTTGTTA CGTGGCCTTG AGCAGACCCC TGCCTCCAGT
    GTGGGGGACC TTGAAAGCTC TGTCACTGGG TCCATGATCA ATGGCTGGGG CTCAGCCTCA
    GAGGAGGACA ACATTTCCAG TGGACGCTCC AGTGTGAGTT CTTCTGATGG CTCCTTCTTC
    ACTGATGCTG ATTTTGCCCA GGCAGTGGCA GCAGCTGCAG AATATGCTGG TCTTAAAGTG
    GCACGTCGTC AGATGCAGGA TGCTGCTGGT CGCCGACATT TTCATGCATC CCAGTGCCCT
    CGACCCACAA GTCCTGTCTC TACAGACAGC AACATGAGTG CTGCTGTAAT GCAGAAGGCC
    AGACCAGCCA AGAAGCAAAA ACACCAGCCA GGACATCTGC GCAGAGAAGT CTACACAGAT
    GATCTACCAC CTCCTCCGGT GCCACCACCT GCTATAAAAT CACCCACTGT CCAATCCAAG
    GCACAGCTGG AGGTGCGACC TGTTATGGTG CCAAAACTCC CTTCTATAGA AGCAAGAACA
    GACCGATCAT CAGATAGAAA AGTAGGCAGT TACAAGGGGA GAGAAGTACT GGATGGAAGA
    CAGGTTGCTG ATATGAGAAC CAATCCAGGT GATACCAGAG AAGCACAGGA ACAGCAAAAT
    GATGGAAAAG GACGAGTAAA CAAGGCAGCA AAACGAGACC TTCCACCAGC AAAGACTCAC
    CTCATCCAAG AGGATATCCT ACCTTACTGT AGACCTACTT TTCCGACATC AAATAATCCC
    AGAGATCCTA GTTCTTCAAG CTCAATGTCA TCAAGAGGAT CAGGAAGCAG ACAAAGAGAA
    CAAGCAAATG TAGGTCGAAG AAATATCGCA GAAATGCAAG TTCTTGGAGG ATATGAAAGA
    GGAGAAGATA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44467
    Clone ID Related Accession (Same CDS sequence) XM_014109754.1
    Accession Version XM_014109754.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4881bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product roundabout homolog 1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAAATGGA AACATGTTCC TTTTTTGGTC ATAATATCAC TCCTCAGCTT GTCCAAAGAT 
    CACCTGTTCC TGGCGCAGCT TATTCCCGGC TCCCGTCTCC GTCAGGAAGA TTTTCCACCT
    CGTATTGTTG AACACCCTTC AGACCTAATT GTGTCAAAAG GAGAACCTGC AACTCTGAAC
    TGTAAAGCTG AAGGCCGCCC CACGCCCACC ATTGAATGGT ACAAAGGTGG GGAGAGAGTG
    GAGACAGACA AAGATGACCC TCGCTCACAC CGAATGTTGC TGCCAAGTGG ATCTTTATTT
    TTCTTACGTA TAGTACATGG ACGAAAAAGT AGGCCTGATG AAGGGGTCTA CGTCTGTGTG
    GCACGGAATT ACCTCGGAGA GGCTGTGAGC CATAATGCAT CGCTGGAGGT GGCTATACTA
    CGGGATGACT TCAGACAAAA TCCTTCTGAT GTCATGGTGG CAGTGGGGGA ACCTGCTGTG
    ATGGAATGCC AACCCCCACG AGGCCATCCT GAACCCACCA TTTCGTGGAA GAAAGATGGC
    TCTCCACTGG ATGATAAAGA TGAAAGAATA ACTATACGAG GTGGAAAGCT CATGATTACT
    TACACCCGCA AAAGTGATGC TGGCAAATAT GTTTGTGTTG GTACCAACAT GGTTGGGGAA
    CGTGAGAGTG AAGTAGCAGA GCTAACTGTC TTGGAGAGAC CATCATTTGT GAAGAGACCT
    AGTAACTTGG CTGTAACTGT GGATGACAGT GCAGAATTTA AATGTGAGGC TCGAGGTGAC
    CCTGTGCCTA CGGTACGATG GAGGAAAGAC GATGGAGAAC TGCCCAAATC CAGATACGAA
    ATCAGAGATG ATCATACTTT GAAGATTCGG AAAGTGATGG CTGGTGACAT GGGGTCATAT
    ACTTGTGTTG CAGAAAATAT GGTTGGAAAA GCTGAAGCAT CAGCTACTCT GACTGTCCAA
    GTTGGGTCTG AACCTCCACA TTTTGTTGTG AAACCTCGTG ACCAGGTTGT TGCCTTGGGG
    CGAACTGTAA CATTTCAGTG TGAAGCAACC GGAAATCCTC AACCAGCTAT TTTTTGGAGA
    AGAGAGGGGA GTCAGAATCT GCTTTTCTCA TATCAGCCGC CACAGTCAAC TAGCCGCTTT
    TCCGTCTCCC AGACTGGTGA TCTCACTATT ACTAATGTCC AGCGGTCTGA TGTTGGTTAT
    TATATCTGCC AGACTTTAAA TGTTGCCGGA AGTATCATCA CAAAAGCATA TTTGGAAGTT
    ACTGATGTGA TTGCAGACCG GCCTCCCCCA GTCATTCGAC AAGGTCCTGT GAATCAGACT
    GTAGCAGTGG ATGGTACTTT AGTGCTCAGC TGTGTGGCCA CAGGCAGTCC AATGCCCACG
    ATTCTCTGGA GAAAGGATGG AGTCCTTGTT TCAACCCAAG ATTCTAGAAT CAAACAGCTG
    GAGACGGGAG TGCTGCAGAT CCGATACGCT AAGCTGGGTG ACACAGGTCG GTACACCTGC
    ATTGCATCAA CCCCCAGTGG TGAAGCAACA TGGAGTGCTT ACATTGAAGT CCAAGAATTT
    GGAGTTCCAG TTCAGCCTCC ACGACCCACC GACCCAAATT TAATCCCTAG TGCCCCCTCA
    AAACCTGAAG TTACAGATGT CAGCAGAAAT ACAGTAACGT TGTCATGGCA ACCAAATTTG
    AATTCAGGTG CAACTCCAAC ATCTTATATT ATAGAAGCCT TCAGCCATGC ATCTGGTAGC
    AGCTGGCAGA CCGTAGCAGA GAATGTGAAA ACAGAAACAT TTGCCATTAA AGGACTCAAA
    CCTAATGCAA TTTACCTTTT CCTTGTGAGG GCAGCAAATG CCTATGGAAT TAGTGATCCA
    AGCCAGATAT CAGATCCAGT GAAAACACAA GATGTTCCCC CAACAAGTCA GGGAGTAGAT
    CACAAGCAGG TCCAGAGAGA ATTGGGAAAC GTCGTACTGC ACCTTCACAA CCCCACCATC
    CTCTCATCCT CTTCCATCGA AGTGCACTGG ACAGTGGACC AACAATCTCA GTATATTCAA
    GGATATAAAA TTCTTTATCG GCCATCTGGA GGCAACCATG GTGAATCGGA GTGGTTAGTT
    TTTGAAGTGA GGACTCCAAC CAAAAATAGC GTGGTTATCC CTGATCTCAA AAAGGGAGTC
    AACTATGAAA TTAAGGCTCG TCCTTTTTTT AATGAATTTC AAGGAGCAGA TAGTGAAATT
    AAATTTGCCA AAACCTTAGA AGAAGCACCC AGTGCCCCAC CCCAAAGTGT AACCGTATCA
    AAGAATGATG GAAATGGAAC TGCAATTCTT GTTAGTTGGC AGCCACCTCC AGAAGACACT
    CAAAATGGAA TGGTCCAAGA GTATAAGGTT TGGTGTCTGG GCAATGAAAC TCGTTATCAC
    ATAAACAAGA CGGTGGATGG TTCCACCTTT TCTGTGGTCA TTCCTTCTCT GGTTCCTGGG
    ATCCGATACA GCGTAGAAGT AGCAGCCAGC ACGGGAGCTG GAGCTGGGGT GAAGAGCGAG
    CCACAGTTCA TCCAGTTAGA TTCTCATGGA AACCCCGTGT CACCTGAAGA CCAAGTCAGC
    CTTGCTCAGC AGATCTCAGA TGTTGTGAAG CAGCCAGCGT TCATTGCGGG AATCGGGGCC
    GCCTGCTGGA TCATCCTCAT GGTCTTTAGC ATCTGGCTCT ATCGACACCG GAAGAAGAGA
    AATGGACTGA CTAGTACTTA TGCGGGTATC AGAAAAGTCC CGTCTTTTAC CTTCACACCA
    ACAGTAACTT ATCAGAGAGG AGGTGAAGCT GTGAGCAGTG GAGGGAGGCC AGGACTTCTG
    AACATCAGCG AACCTGCCAC ACAGCCCTGG CTGGCAGACA CGTGGCCCAA TACTGGCAAC
    AATCACAATG ACTGCTCTAT CAATTGCTGT ACGGCAGGCA ATGGTAACAG TGATAACAAC
    CTGACCACCT ACAGTCGCCC AGCCGATTGT ATAGCAAATT ATAACAACCA ACTGGATAAC
    AAACAAACAA ATCTGATGCT CCCTGAGTCA ACTGTTTATG GTGATGTGGA CCTTAGTAAC
    AAAATCAATG AGATGAAAAC CTTCAATAGC CCAAATCTGA AGGATGGGCG TTTTGTCAAT
    CCATCAGGGC AGCCCACTCC TTATGCCACC ACCCAGCTCA TCCAGTCAAA CCTCAGCAAC
    AACATGAACA ATGGCAGCGG GGACTCCAGC GAGAAGCACT GGAAACCTCC CGGCCAGCAG
    AAACAAGAAG TGCCACCAGT TCAGTATAAC ATCATGGAGC AAAACAAACT GAACAAAGAT
    TATCGAGCAA ATGACACAGT TCCTCCAACT ATCCCATACA ACCAATCATA TGACCAGAAT
    ACAGGAGGAT CCTACAACAG TTCAGACCGG GGCAGTAGTA CATCTGGGAG TCAAGGGCAC
    AAGAAAGGGG CACGGACACC CAAAGTACCA AAACAAGGTG GCATGAACTG GGCAGACCTG
    CTTCCTCCTC CCCCAGCGCA TCCTCCTCCT CATAGCAATA GTGAAGAGTA CAATATTTCT
    GTAGATGAAA GCTATGACCA AGAAATGCCA TGTCCCGTGC CACCAGCAAG GATGTATTTG
    CAACAGAATG AACTAGAAGA GGAGGAAGAT GAGCGAGGCC CCACTCCCCC TGTGCGGGGA
    GCAGCTTCTT CTCCAGCTGC TGTGTCTTAT AGCCATCAGT CCACTGCTAC TCTGACCCCC
    TCCCCACAAG AAGAACTCCA GCCCATGTTG CAGGACTGCC CGGAGGAGAT TGGCCACATG
    CAGCACCAAC CCGATAGGAG ACGGCAGCCT GTGAGTCCTC CTCCACCACC AAGACCGATT
    TCCCCACCAC ATACCTATGG CTACATTTCA GGACCTCTTG TCTCAGATAT GGATACAGAT
    GCACCAGAAG AGGAAGAAGA TGAAGCAGAC ATGGAAGTGG CCAAGATGCA GACCAGACGG
    CTTTTGTTAC GTGGCCTTGA GCAGACCCCT GCCTCCAGTG TGGGGGACCT TGAAAGCTCT
    GTCACTGGGT CCATGATCAA TGGCTGGGGC TCAGCCTCAG AGGAGGACAA CATTTCCAGT
    GGACGCTCCA GTGTGAGTTC TTCTGATGGC TCCTTCTTCA CTGATGCTGA TTTTGCCCAG
    GCAGTGGCAG CAGCTGCAGA ATATGCTGGT CTTAAAGTGG CACGTCGTCA GATGCAGGAT
    GCTGCTGGTC GCCGACATTT TCATGCATCC CAGTGCCCTC GACCCACAAG TCCTGTCTCT
    ACAGACAGCA ACATGAGTGC TGCTGTAATG CAGAAGGCCA GACCAGCCAA GAAGCAAAAA
    CACCAGCCAG GACATCTGCG CAGAGAAGTC TACACAGATG ATCTACCACC TCCTCCGGTG
    CCACCACCTG CTATAAAATC ACCCACTGTC CAATCCAAGG CACAGCTGGA GGTGCGACCT
    GTTATGGTGC CAAAACTCCC TTCTATAGAA GCAAGAACAG ACCGATCATC AGATAGAAAA
    GTAGGCAGTT ACAAGGGGAG AGAAGTACTG GATGGAAGAC AGGTTGCTGA TATGAGAACC
    AATCCAGGTG ATACCAGAGA AGCACAGGAA CAGCAAAATG ATGGAAAAGG ACGAGTAAAC
    AAGGCAGCAA AACGAGACCT TCCACCAGCA AAGACTCACC TCATCCAAGA GGATATCCTA
    CCTTACTGTA GACCTACTTT TCCGACATCA AATAATCCCA GAGATCCTAG TTCTTCAAGC
    TCAATGTCAT CAAGAGGATC AGGAAGCAGA CAAAGAGAAC AAGCAAATGT AGGTCGAAGA
    AATATCGCAG AAATGCAAGT TCTTGGAGGA TATGAAAGAG GAGAAGATAA TAATGAAGAA
    TTAGAGGAAA CTGAAAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965229.1
    CDS303..5183
    Translation

    Target ORF information:

    RefSeq Version XM_014109754.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109754.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAAATGGA AACATGTTCC TTTTTTGGTC ATAATATCAC TCCTCAGCTT GTCCAAAGAT 
    CACCTGTTCC TGGCGCAGCT TATTCCCGGC TCCCGTCTCC GTCAGGAAGA TTTTCCACCT
    CGTATTGTTG AACACCCTTC AGACCTAATT GTGTCAAAAG GAGAACCTGC AACTCTGAAC
    TGTAAAGCTG AAGGCCGCCC CACGCCCACC ATTGAATGGT ACAAAGGTGG GGAGAGAGTG
    GAGACAGACA AAGATGACCC TCGCTCACAC CGAATGTTGC TGCCAAGTGG ATCTTTATTT
    TTCTTACGTA TAGTACATGG ACGAAAAAGT AGGCCTGATG AAGGGGTCTA CGTCTGTGTG
    GCACGGAATT ACCTCGGAGA GGCTGTGAGC CATAATGCAT CGCTGGAGGT GGCTATACTA
    CGGGATGACT TCAGACAAAA TCCTTCTGAT GTCATGGTGG CAGTGGGGGA ACCTGCTGTG
    ATGGAATGCC AACCCCCACG AGGCCATCCT GAACCCACCA TTTCGTGGAA GAAAGATGGC
    TCTCCACTGG ATGATAAAGA TGAAAGAATA ACTATACGAG GTGGAAAGCT CATGATTACT
    TACACCCGCA AAAGTGATGC TGGCAAATAT GTTTGTGTTG GTACCAACAT GGTTGGGGAA
    CGTGAGAGTG AAGTAGCAGA GCTAACTGTC TTGGAGAGAC CATCATTTGT GAAGAGACCT
    AGTAACTTGG CTGTAACTGT GGATGACAGT GCAGAATTTA AATGTGAGGC TCGAGGTGAC
    CCTGTGCCTA CGGTACGATG GAGGAAAGAC GATGGAGAAC TGCCCAAATC CAGATACGAA
    ATCAGAGATG ATCATACTTT GAAGATTCGG AAAGTGATGG CTGGTGACAT GGGGTCATAT
    ACTTGTGTTG CAGAAAATAT GGTTGGAAAA GCTGAAGCAT CAGCTACTCT GACTGTCCAA
    GTTGGGTCTG AACCTCCACA TTTTGTTGTG AAACCTCGTG ACCAGGTTGT TGCCTTGGGG
    CGAACTGTAA CATTTCAGTG TGAAGCAACC GGAAATCCTC AACCAGCTAT TTTTTGGAGA
    AGAGAGGGGA GTCAGAATCT GCTTTTCTCA TATCAGCCGC CACAGTCAAC TAGCCGCTTT
    TCCGTCTCCC AGACTGGTGA TCTCACTATT ACTAATGTCC AGCGGTCTGA TGTTGGTTAT
    TATATCTGCC AGACTTTAAA TGTTGCCGGA AGTATCATCA CAAAAGCATA TTTGGAAGTT
    ACTGATGTGA TTGCAGACCG GCCTCCCCCA GTCATTCGAC AAGGTCCTGT GAATCAGACT
    GTAGCAGTGG ATGGTACTTT AGTGCTCAGC TGTGTGGCCA CAGGCAGTCC AATGCCCACG
    ATTCTCTGGA GAAAGGATGG AGTCCTTGTT TCAACCCAAG ATTCTAGAAT CAAACAGCTG
    GAGACGGGAG TGCTGCAGAT CCGATACGCT AAGCTGGGTG ACACAGGTCG GTACACCTGC
    ATTGCATCAA CCCCCAGTGG TGAAGCAACA TGGAGTGCTT ACATTGAAGT CCAAGAATTT
    GGAGTTCCAG TTCAGCCTCC ACGACCCACC GACCCAAATT TAATCCCTAG TGCCCCCTCA
    AAACCTGAAG TTACAGATGT CAGCAGAAAT ACAGTAACGT TGTCATGGCA ACCAAATTTG
    AATTCAGGTG CAACTCCAAC ATCTTATATT ATAGAAGCCT TCAGCCATGC ATCTGGTAGC
    AGCTGGCAGA CCGTAGCAGA GAATGTGAAA ACAGAAACAT TTGCCATTAA AGGACTCAAA
    CCTAATGCAA TTTACCTTTT CCTTGTGAGG GCAGCAAATG CCTATGGAAT TAGTGATCCA
    AGCCAGATAT CAGATCCAGT GAAAACACAA GATGTTCCCC CAACAAGTCA GGGAGTAGAT
    CACAAGCAGG TCCAGAGAGA ATTGGGAAAC GTCGTACTGC ACCTTCACAA CCCCACCATC
    CTCTCATCCT CTTCCATCGA AGTGCACTGG ACAGTGGACC AACAATCTCA GTATATTCAA
    GGATATAAAA TTCTTTATCG GCCATCTGGA GGCAACCATG GTGAATCGGA GTGGTTAGTT
    TTTGAAGTGA GGACTCCAAC CAAAAATAGC GTGGTTATCC CTGATCTCAA AAAGGGAGTC
    AACTATGAAA TTAAGGCTCG TCCTTTTTTT AATGAATTTC AAGGAGCAGA TAGTGAAATT
    AAATTTGCCA AAACCTTAGA AGAAGCACCC AGTGCCCCAC CCCAAAGTGT AACCGTATCA
    AAGAATGATG GAAATGGAAC TGCAATTCTT GTTAGTTGGC AGCCACCTCC AGAAGACACT
    CAAAATGGAA TGGTCCAAGA GTATAAGGTT TGGTGTCTGG GCAATGAAAC TCGTTATCAC
    ATAAACAAGA CGGTGGATGG TTCCACCTTT TCTGTGGTCA TTCCTTCTCT GGTTCCTGGG
    ATCCGATACA GCGTAGAAGT AGCAGCCAGC ACGGGAGCTG GAGCTGGGGT GAAGAGCGAG
    CCACAGTTCA TCCAGTTAGA TTCTCATGGA AACCCCGTGT CACCTGAAGA CCAAGTCAGC
    CTTGCTCAGC AGATCTCAGA TGTTGTGAAG CAGCCAGCGT TCATTGCGGG AATCGGGGCC
    GCCTGCTGGA TCATCCTCAT GGTCTTTAGC ATCTGGCTCT ATCGACACCG GAAGAAGAGA
    AATGGACTGA CTAGTACTTA TGCGGGTATC AGAAAAGTCC CGTCTTTTAC CTTCACACCA
    ACAGTAACTT ATCAGAGAGG AGGTGAAGCT GTGAGCAGTG GAGGGAGGCC AGGACTTCTG
    AACATCAGCG AACCTGCCAC ACAGCCCTGG CTGGCAGACA CGTGGCCCAA TACTGGCAAC
    AATCACAATG ACTGCTCTAT CAATTGCTGT ACGGCAGGCA ATGGTAACAG TGATAACAAC
    CTGACCACCT ACAGTCGCCC AGCCGATTGT ATAGCAAATT ATAACAACCA ACTGGATAAC
    AAACAAACAA ATCTGATGCT CCCTGAGTCA ACTGTTTATG GTGATGTGGA CCTTAGTAAC
    AAAATCAATG AGATGAAAAC CTTCAATAGC CCAAATCTGA AGGATGGGCG TTTTGTCAAT
    CCATCAGGGC AGCCCACTCC TTATGCCACC ACCCAGCTCA TCCAGTCAAA CCTCAGCAAC
    AACATGAACA ATGGCAGCGG GGACTCCAGC GAGAAGCACT GGAAACCTCC CGGCCAGCAG
    AAACAAGAAG TGCCACCAGT TCAGTATAAC ATCATGGAGC AAAACAAACT GAACAAAGAT
    TATCGAGCAA ATGACACAGT TCCTCCAACT ATCCCATACA ACCAATCATA TGACCAGAAT
    ACAGGAGGAT CCTACAACAG TTCAGACCGG GGCAGTAGTA CATCTGGGAG TCAAGGGCAC
    AAGAAAGGGG CACGGACACC CAAAGTACCA AAACAAGGTG GCATGAACTG GGCAGACCTG
    CTTCCTCCTC CCCCAGCGCA TCCTCCTCCT CATAGCAATA GTGAAGAGTA CAATATTTCT
    GTAGATGAAA GCTATGACCA AGAAATGCCA TGTCCCGTGC CACCAGCAAG GATGTATTTG
    CAACAGAATG AACTAGAAGA GGAGGAAGAT GAGCGAGGCC CCACTCCCCC TGTGCGGGGA
    GCAGCTTCTT CTCCAGCTGC TGTGTCTTAT AGCCATCAGT CCACTGCTAC TCTGACCCCC
    TCCCCACAAG AAGAACTCCA GCCCATGTTG CAGGACTGCC CGGAGGAGAT TGGCCACATG
    CAGCACCAAC CCGATAGGAG ACGGCAGCCT GTGAGTCCTC CTCCACCACC AAGACCGATT
    TCCCCACCAC ATACCTATGG CTACATTTCA GGACCTCTTG TCTCAGATAT GGATACAGAT
    GCACCAGAAG AGGAAGAAGA TGAAGCAGAC ATGGAAGTGG CCAAGATGCA GACCAGACGG
    CTTTTGTTAC GTGGCCTTGA GCAGACCCCT GCCTCCAGTG TGGGGGACCT TGAAAGCTCT
    GTCACTGGGT CCATGATCAA TGGCTGGGGC TCAGCCTCAG AGGAGGACAA CATTTCCAGT
    GGACGCTCCA GTGTGAGTTC TTCTGATGGC TCCTTCTTCA CTGATGCTGA TTTTGCCCAG
    GCAGTGGCAG CAGCTGCAGA ATATGCTGGT CTTAAAGTGG CACGTCGTCA GATGCAGGAT
    GCTGCTGGTC GCCGACATTT TCATGCATCC CAGTGCCCTC GACCCACAAG TCCTGTCTCT
    ACAGACAGCA ACATGAGTGC TGCTGTAATG CAGAAGGCCA GACCAGCCAA GAAGCAAAAA
    CACCAGCCAG GACATCTGCG CAGAGAAGTC TACACAGATG ATCTACCACC TCCTCCGGTG
    CCACCACCTG CTATAAAATC ACCCACTGTC CAATCCAAGG CACAGCTGGA GGTGCGACCT
    GTTATGGTGC CAAAACTCCC TTCTATAGAA GCAAGAACAG ACCGATCATC AGATAGAAAA
    GTAGGCAGTT ACAAGGGGAG AGAAGTACTG GATGGAAGAC AGGTTGCTGA TATGAGAACC
    AATCCAGGTG ATACCAGAGA AGCACAGGAA CAGCAAAATG ATGGAAAAGG ACGAGTAAAC
    AAGGCAGCAA AACGAGACCT TCCACCAGCA AAGACTCACC TCATCCAAGA GGATATCCTA
    CCTTACTGTA GACCTACTTT TCCGACATCA AATAATCCCA GAGATCCTAG TTCTTCAAGC
    TCAATGTCAT CAAGAGGATC AGGAAGCAGA CAAAGAGAAC AAGCAAATGT AGGTCGAAGA
    AATATCGCAG AAATGCAAGT TCTTGGAGGA TATGAAAGAG GAGAAGATAA TAATGAAGAA
    TTAGAGGAAA CTGAAAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44468
    Clone ID Related Accession (Same CDS sequence) XM_014109755.1 , XM_014109755.2
    Accession Version XM_014109755.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product roundabout homolog 1 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCCGCCCG CCTGTACCTC TTCGGATTGC TCTGTCTCTG CTCAGGCTCC 
    CGTCTCCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCTTCAGA CCTAATTGTG
    TCAAAAGGAG AACCTGCAAC TCTGAACTGT AAAGCTGAAG GCCGCCCCAC GCCCACCATT
    GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA
    ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG AAAAAGTAGG
    CCTGATGAAG GGGTCTACGT CTGTGTGGCA CGGAATTACC TCGGAGAGGC TGTGAGCCAT
    AATGCATCGC TGGAGGTGGC TATACTACGG GATGACTTCA GACAAAATCC TTCTGATGTC
    ATGGTGGCAG TGGGGGAACC TGCTGTGATG GAATGCCAAC CCCCACGAGG CCATCCTGAA
    CCCACCATTT CGTGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT
    ATACGAGGTG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCTGG CAAATATGTT
    TGTGTTGGTA CCAACATGGT TGGGGAACGT GAGAGTGAAG TAGCAGAGCT AACTGTCTTG
    GAGAGACCAT CATTTGTGAA GAGACCTAGT AACTTGGCTG TAACTGTGGA TGACAGTGCA
    GAATTTAAAT GTGAGGCTCG AGGTGACCCT GTGCCTACGG TACGATGGAG GAAAGACGAT
    GGAGAACTGC CCAAATCCAG ATACGAAATC AGAGATGATC ATACTTTGAA GATTCGGAAA
    GTGATGGCTG GTGACATGGG GTCATATACT TGTGTTGCAG AAAATATGGT TGGAAAAGCT
    GAAGCATCAG CTACTCTGAC TGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA
    CCTCGTGACC AGGTTGTTGC CTTGGGGCGA ACTGTAACAT TTCAGTGTGA AGCAACCGGA
    AATCCTCAAC CAGCTATTTT TTGGAGAAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT
    CAGCCGCCAC AGTCAACTAG CCGCTTTTCC GTCTCCCAGA CTGGTGATCT CACTATTACT
    AATGTCCAGC GGTCTGATGT TGGTTATTAT ATCTGCCAGA CTTTAAATGT TGCCGGAAGT
    ATCATCACAA AAGCATATTT GGAAGTTACT GATGTGATTG CAGACCGGCC TCCCCCAGTC
    ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT
    GTGGCCACAG GCAGTCCAAT GCCCACGATT CTCTGGAGAA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTAGAATCAA ACAGCTGGAG ACGGGAGTGC TGCAGATCCG ATACGCTAAG
    CTGGGTGACA CAGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG
    AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCACG ACCCACCGAC
    CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CAGAAATACA
    GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCAACATC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA
    GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA
    GCAAATGCCT ATGGAATTAG TGATCCAAGC CAGATATCAG ATCCAGTGAA AACACAAGAT
    GTTCCCCCAA CAAGTCAGGG AGTAGATCAC AAGCAGGTCC AGAGAGAATT GGGAAACGTC
    GTACTGCACC TTCACAACCC CACCATCCTC TCATCCTCTT CCATCGAAGT GCACTGGACA
    GTGGACCAAC AATCTCAGTA TATTCAAGGA TATAAAATTC TTTATCGGCC ATCTGGAGGC
    AACCATGGTG AATCGGAGTG GTTAGTTTTT GAAGTGAGGA CTCCAACCAA AAATAGCGTG
    GTTATCCCTG ATCTCAAAAA GGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT
    GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCTTAGAAGA AGCACCCAGT
    GCCCCACCCC AAAGTGTAAC CGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT
    AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG
    TGTCTGGGCA ATGAAACTCG TTATCACATA AACAAGACGG TGGATGGTTC CACCTTTTCT
    GTGGTCATTC CTTCTCTGGT TCCTGGGATC CGATACAGCG TAGAAGTAGC AGCCAGCACG
    GGAGCTGGAG CTGGGGTGAA GAGCGAGCCA CAGTTCATCC AGTTAGATTC TCATGGAAAC
    CCCGTGTCAC CTGAAGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGATGT TGTGAAGCAG
    CCAGCGTTCA TTGCGGGAAT CGGGGCCGCC TGCTGGATCA TCCTCATGGT CTTTAGCATC
    TGGCTCTATC GACACCGGAA GAAGAGAAAT GGACTGACTA GTACTTATGC GGGTATCAGA
    AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTATC AGAGAGGAGG TGAAGCTGTG
    AGCAGTGGAG GGAGGCCAGG ACTTCTGAAC ATCAGCGAAC CTGCCACACA GCCCTGGCTG
    GCAGACACGT GGCCCAATAC TGGCAACAAT CACAATGACT GCTCTATCAA TTGCTGTACG
    GCAGGCAATG GTAACAGTGA TAACAACCTG ACCACCTACA GTCGCCCAGC CGATTGTATA
    GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT
    GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA
    AATCTGAAGG ATGGGCGTTT TGTCAATCCA TCAGGGCAGC CCACTCCTTA TGCCACCACC
    CAGCTCATCC AGTCAAACCT CAGCAACAAC ATGAACAATG GCAGCGGGGA CTCCAGCGAG
    AAGCACTGGA AACCTCCCGG CCAGCAGAAA CAAGAAGTGC CACCAGTTCA GTATAACATC
    ATGGAGCAAA ACAAACTGAA CAAAGATTAT CGAGCAAATG ACACAGTTCC TCCAACTATC
    CCATACAACC AATCATATGA CCAGAATACA GGAGGATCCT ACAACAGTTC AGACCGGGGC
    AGTAGTACAT CTGGGAGTCA AGGGCACAAG AAAGGGGCAC GGACACCCAA AGTACCAAAA
    CAAGGTGGCA TGAACTGGGC AGACCTGCTT CCTCCTCCCC CAGCGCATCC TCCTCCTCAT
    AGCAATAGTG AAGAGTACAA TATTTCTGTA GATGAAAGCT ATGACCAAGA AATGCCATGT
    CCCGTGCCAC CAGCAAGGAT GTATTTGCAA CAGAATGAAC TAGAAGAGGA GGAAGATGAG
    CGAGGCCCCA CTCCCCCTGT GCGGGGAGCA GCTTCTTCTC CAGCTGCTGT GTCTTATAGC
    CATCAGTCCA CTGCTACTCT GACCCCCTCC CCACAAGAAG AACTCCAGCC CATGTTGCAG
    GACTGCCCGG AGGAGATTGG CCACATGCAG CACCAACCCG ATAGGAGACG GCAGCCTGTG
    AGTCCTCCTC CACCACCAAG ACCGATTTCC CCACCACATA CCTATGGCTA CATTTCAGGA
    CCTCTTGTCT CAGATATGGA TACAGATGCA CCAGAAGAGG AAGAAGATGA AGCAGACATG
    GAAGTGGCCA AGATGCAGAC CAGACGGCTT TTGTTACGTG GCCTTGAGCA GACCCCTGCC
    TCCAGTGTGG GGGACCTTGA AAGCTCTGTC ACTGGGTCCA TGATCAATGG CTGGGGCTCA
    GCCTCAGAGG AGGACAACAT TTCCAGTGGA CGCTCCAGTG TGAGTTCTTC TGATGGCTCC
    TTCTTCACTG ATGCTGATTT TGCCCAGGCA GTGGCAGCAG CTGCAGAATA TGCTGGTCTT
    AAAGTGGCAC GTCGTCAGAT GCAGGATGCT GCTGGTCGCC GACATTTTCA TGCATCCCAG
    TGCCCTCGAC CCACAAGTCC TGTCTCTACA GACAGCAACA TGAGTGCTGC TGTAATGCAG
    AAGGCCAGAC CAGCCAAGAA GCAAAAACAC CAGCCAGGAC ATCTGCGCAG AGAAGTCTAC
    ACAGATGATC TACCACCTCC TCCGGTGCCA CCACCTGCTA TAAAATCACC CACTGTCCAA
    TCCAAGGCAC AGCTGGAGGT GCGACCTGTT ATGGTGCCAA AACTCCCTTC TATAGAAGCA
    AGAACAGACC GATCATCAGA TAGAAAAGTA GGCAGTTACA AGGGGAGAGA AGTACTGGAT
    GGAAGACAGG TTGCTGATAT GAGAACCAAT CCAGGTGATA CCAGAGAAGC ACAGGAACAG
    CAAAATGATG GAAAAGGACG AGTAAACAAG GCAGCAAAAC GAGACCTTCC ACCAGCAAAG
    ACTCACCTCA TCCAAGAGGA TATCCTACCT TACTGTAGAC CTACTTTTCC GACATCAAAT
    AATCCCAGAG ATCCTAGTTC TTCAAGCTCA ATGTCATCAA GAGGATCAGG AAGCAGACAA
    AGAGAACAAG CAAATGTAGG TCGAAGAAAT ATCGCAGAAA TGCAAGTTCT TGGAGGATAT
    GAAAGAGGAG AAGATAATAA TGAAGAATTA GAGGAAACTG AAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965230.1
    CDS369..5216
    Translation

    Target ORF information:

    RefSeq Version XM_014109755.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109755.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCCGCCCG CCTGTACCTC TTCGGATTGC TCTGTCTCTG CTCAGGCTCC 
    CGTCTCCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCTTCAGA CCTAATTGTG
    TCAAAAGGAG AACCTGCAAC TCTGAACTGT AAAGCTGAAG GCCGCCCCAC GCCCACCATT
    GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA
    ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG AAAAAGTAGG
    CCTGATGAAG GGGTCTACGT CTGTGTGGCA CGGAATTACC TCGGAGAGGC TGTGAGCCAT
    AATGCATCGC TGGAGGTGGC TATACTACGG GATGACTTCA GACAAAATCC TTCTGATGTC
    ATGGTGGCAG TGGGGGAACC TGCTGTGATG GAATGCCAAC CCCCACGAGG CCATCCTGAA
    CCCACCATTT CGTGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT
    ATACGAGGTG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCTGG CAAATATGTT
    TGTGTTGGTA CCAACATGGT TGGGGAACGT GAGAGTGAAG TAGCAGAGCT AACTGTCTTG
    GAGAGACCAT CATTTGTGAA GAGACCTAGT AACTTGGCTG TAACTGTGGA TGACAGTGCA
    GAATTTAAAT GTGAGGCTCG AGGTGACCCT GTGCCTACGG TACGATGGAG GAAAGACGAT
    GGAGAACTGC CCAAATCCAG ATACGAAATC AGAGATGATC ATACTTTGAA GATTCGGAAA
    GTGATGGCTG GTGACATGGG GTCATATACT TGTGTTGCAG AAAATATGGT TGGAAAAGCT
    GAAGCATCAG CTACTCTGAC TGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA
    CCTCGTGACC AGGTTGTTGC CTTGGGGCGA ACTGTAACAT TTCAGTGTGA AGCAACCGGA
    AATCCTCAAC CAGCTATTTT TTGGAGAAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT
    CAGCCGCCAC AGTCAACTAG CCGCTTTTCC GTCTCCCAGA CTGGTGATCT CACTATTACT
    AATGTCCAGC GGTCTGATGT TGGTTATTAT ATCTGCCAGA CTTTAAATGT TGCCGGAAGT
    ATCATCACAA AAGCATATTT GGAAGTTACT GATGTGATTG CAGACCGGCC TCCCCCAGTC
    ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT
    GTGGCCACAG GCAGTCCAAT GCCCACGATT CTCTGGAGAA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTAGAATCAA ACAGCTGGAG ACGGGAGTGC TGCAGATCCG ATACGCTAAG
    CTGGGTGACA CAGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG
    AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCACG ACCCACCGAC
    CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CAGAAATACA
    GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCAACATC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA
    GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA
    GCAAATGCCT ATGGAATTAG TGATCCAAGC CAGATATCAG ATCCAGTGAA AACACAAGAT
    GTTCCCCCAA CAAGTCAGGG AGTAGATCAC AAGCAGGTCC AGAGAGAATT GGGAAACGTC
    GTACTGCACC TTCACAACCC CACCATCCTC TCATCCTCTT CCATCGAAGT GCACTGGACA
    GTGGACCAAC AATCTCAGTA TATTCAAGGA TATAAAATTC TTTATCGGCC ATCTGGAGGC
    AACCATGGTG AATCGGAGTG GTTAGTTTTT GAAGTGAGGA CTCCAACCAA AAATAGCGTG
    GTTATCCCTG ATCTCAAAAA GGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT
    GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCTTAGAAGA AGCACCCAGT
    GCCCCACCCC AAAGTGTAAC CGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT
    AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG
    TGTCTGGGCA ATGAAACTCG TTATCACATA AACAAGACGG TGGATGGTTC CACCTTTTCT
    GTGGTCATTC CTTCTCTGGT TCCTGGGATC CGATACAGCG TAGAAGTAGC AGCCAGCACG
    GGAGCTGGAG CTGGGGTGAA GAGCGAGCCA CAGTTCATCC AGTTAGATTC TCATGGAAAC
    CCCGTGTCAC CTGAAGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGATGT TGTGAAGCAG
    CCAGCGTTCA TTGCGGGAAT CGGGGCCGCC TGCTGGATCA TCCTCATGGT CTTTAGCATC
    TGGCTCTATC GACACCGGAA GAAGAGAAAT GGACTGACTA GTACTTATGC GGGTATCAGA
    AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTATC AGAGAGGAGG TGAAGCTGTG
    AGCAGTGGAG GGAGGCCAGG ACTTCTGAAC ATCAGCGAAC CTGCCACACA GCCCTGGCTG
    GCAGACACGT GGCCCAATAC TGGCAACAAT CACAATGACT GCTCTATCAA TTGCTGTACG
    GCAGGCAATG GTAACAGTGA TAACAACCTG ACCACCTACA GTCGCCCAGC CGATTGTATA
    GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT
    GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA
    AATCTGAAGG ATGGGCGTTT TGTCAATCCA TCAGGGCAGC CCACTCCTTA TGCCACCACC
    CAGCTCATCC AGTCAAACCT CAGCAACAAC ATGAACAATG GCAGCGGGGA CTCCAGCGAG
    AAGCACTGGA AACCTCCCGG CCAGCAGAAA CAAGAAGTGC CACCAGTTCA GTATAACATC
    ATGGAGCAAA ACAAACTGAA CAAAGATTAT CGAGCAAATG ACACAGTTCC TCCAACTATC
    CCATACAACC AATCATATGA CCAGAATACA GGAGGATCCT ACAACAGTTC AGACCGGGGC
    AGTAGTACAT CTGGGAGTCA AGGGCACAAG AAAGGGGCAC GGACACCCAA AGTACCAAAA
    CAAGGTGGCA TGAACTGGGC AGACCTGCTT CCTCCTCCCC CAGCGCATCC TCCTCCTCAT
    AGCAATAGTG AAGAGTACAA TATTTCTGTA GATGAAAGCT ATGACCAAGA AATGCCATGT
    CCCGTGCCAC CAGCAAGGAT GTATTTGCAA CAGAATGAAC TAGAAGAGGA GGAAGATGAG
    CGAGGCCCCA CTCCCCCTGT GCGGGGAGCA GCTTCTTCTC CAGCTGCTGT GTCTTATAGC
    CATCAGTCCA CTGCTACTCT GACCCCCTCC CCACAAGAAG AACTCCAGCC CATGTTGCAG
    GACTGCCCGG AGGAGATTGG CCACATGCAG CACCAACCCG ATAGGAGACG GCAGCCTGTG
    AGTCCTCCTC CACCACCAAG ACCGATTTCC CCACCACATA CCTATGGCTA CATTTCAGGA
    CCTCTTGTCT CAGATATGGA TACAGATGCA CCAGAAGAGG AAGAAGATGA AGCAGACATG
    GAAGTGGCCA AGATGCAGAC CAGACGGCTT TTGTTACGTG GCCTTGAGCA GACCCCTGCC
    TCCAGTGTGG GGGACCTTGA AAGCTCTGTC ACTGGGTCCA TGATCAATGG CTGGGGCTCA
    GCCTCAGAGG AGGACAACAT TTCCAGTGGA CGCTCCAGTG TGAGTTCTTC TGATGGCTCC
    TTCTTCACTG ATGCTGATTT TGCCCAGGCA GTGGCAGCAG CTGCAGAATA TGCTGGTCTT
    AAAGTGGCAC GTCGTCAGAT GCAGGATGCT GCTGGTCGCC GACATTTTCA TGCATCCCAG
    TGCCCTCGAC CCACAAGTCC TGTCTCTACA GACAGCAACA TGAGTGCTGC TGTAATGCAG
    AAGGCCAGAC CAGCCAAGAA GCAAAAACAC CAGCCAGGAC ATCTGCGCAG AGAAGTCTAC
    ACAGATGATC TACCACCTCC TCCGGTGCCA CCACCTGCTA TAAAATCACC CACTGTCCAA
    TCCAAGGCAC AGCTGGAGGT GCGACCTGTT ATGGTGCCAA AACTCCCTTC TATAGAAGCA
    AGAACAGACC GATCATCAGA TAGAAAAGTA GGCAGTTACA AGGGGAGAGA AGTACTGGAT
    GGAAGACAGG TTGCTGATAT GAGAACCAAT CCAGGTGATA CCAGAGAAGC ACAGGAACAG
    CAAAATGATG GAAAAGGACG AGTAAACAAG GCAGCAAAAC GAGACCTTCC ACCAGCAAAG
    ACTCACCTCA TCCAAGAGGA TATCCTACCT TACTGTAGAC CTACTTTTCC GACATCAAAT
    AATCCCAGAG ATCCTAGTTC TTCAAGCTCA ATGTCATCAA GAGGATCAGG AAGCAGACAA
    AGAGAACAAG CAAATGTAGG TCGAAGAAAT ATCGCAGAAA TGCAAGTTCT TGGAGGATAT
    GAAAGAGGAG AAGATAATAA TGAAGAATTA GAGGAAACTG AAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44468
    Clone ID Related Accession (Same CDS sequence) XM_014109755.1 , XM_014109755.2
    Accession Version XM_014109755.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product roundabout homolog 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109755.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCCGCCCG CCTGTACCTC TTCGGATTGC TCTGTCTCTG CTCAGGCTCC 
    CGTCTCCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCTTCAGA CCTAATTGTG
    TCAAAAGGAG AACCTGCAAC TCTGAACTGT AAAGCTGAAG GCCGCCCCAC GCCCACCATT
    GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA
    ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG AAAAAGTAGG
    CCTGATGAAG GGGTCTACGT CTGTGTGGCA CGGAATTACC TCGGAGAGGC TGTGAGCCAT
    AATGCATCGC TGGAGGTGGC TATACTACGG GATGACTTCA GACAAAATCC TTCTGATGTC
    ATGGTGGCAG TGGGGGAACC TGCTGTGATG GAATGCCAAC CCCCACGAGG CCATCCTGAA
    CCCACCATTT CGTGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT
    ATACGAGGTG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCTGG CAAATATGTT
    TGTGTTGGTA CCAACATGGT TGGGGAACGT GAGAGTGAAG TAGCAGAGCT AACTGTCTTG
    GAGAGACCAT CATTTGTGAA GAGACCTAGT AACTTGGCTG TAACTGTGGA TGACAGTGCA
    GAATTTAAAT GTGAGGCTCG AGGTGACCCT GTGCCTACGG TACGATGGAG GAAAGACGAT
    GGAGAACTGC CCAAATCCAG ATACGAAATC AGAGATGATC ATACTTTGAA GATTCGGAAA
    GTGATGGCTG GTGACATGGG GTCATATACT TGTGTTGCAG AAAATATGGT TGGAAAAGCT
    GAAGCATCAG CTACTCTGAC TGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA
    CCTCGTGACC AGGTTGTTGC CTTGGGGCGA ACTGTAACAT TTCAGTGTGA AGCAACCGGA
    AATCCTCAAC CAGCTATTTT TTGGAGAAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT
    CAGCCGCCAC AGTCAACTAG CCGCTTTTCC GTCTCCCAGA CTGGTGATCT CACTATTACT
    AATGTCCAGC GGTCTGATGT TGGTTATTAT ATCTGCCAGA CTTTAAATGT TGCCGGAAGT
    ATCATCACAA AAGCATATTT GGAAGTTACT GATGTGATTG CAGACCGGCC TCCCCCAGTC
    ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT
    GTGGCCACAG GCAGTCCAAT GCCCACGATT CTCTGGAGAA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTAGAATCAA ACAGCTGGAG ACGGGAGTGC TGCAGATCCG ATACGCTAAG
    CTGGGTGACA CAGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG
    AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCACG ACCCACCGAC
    CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CAGAAATACA
    GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCAACATC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA
    GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA
    GCAAATGCCT ATGGAATTAG TGATCCAAGC CAGATATCAG ATCCAGTGAA AACACAAGAT
    GTTCCCCCAA CAAGTCAGGG AGTAGATCAC AAGCAGGTCC AGAGAGAATT GGGAAACGTC
    GTACTGCACC TTCACAACCC CACCATCCTC TCATCCTCTT CCATCGAAGT GCACTGGACA
    GTGGACCAAC AATCTCAGTA TATTCAAGGA TATAAAATTC TTTATCGGCC ATCTGGAGGC
    AACCATGGTG AATCGGAGTG GTTAGTTTTT GAAGTGAGGA CTCCAACCAA AAATAGCGTG
    GTTATCCCTG ATCTCAAAAA GGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT
    GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCTTAGAAGA AGCACCCAGT
    GCCCCACCCC AAAGTGTAAC CGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT
    AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG
    TGTCTGGGCA ATGAAACTCG TTATCACATA AACAAGACGG TGGATGGTTC CACCTTTTCT
    GTGGTCATTC CTTCTCTGGT TCCTGGGATC CGATACAGCG TAGAAGTAGC AGCCAGCACG
    GGAGCTGGAG CTGGGGTGAA GAGCGAGCCA CAGTTCATCC AGTTAGATTC TCATGGAAAC
    CCCGTGTCAC CTGAAGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGATGT TGTGAAGCAG
    CCAGCGTTCA TTGCGGGAAT CGGGGCCGCC TGCTGGATCA TCCTCATGGT CTTTAGCATC
    TGGCTCTATC GACACCGGAA GAAGAGAAAT GGACTGACTA GTACTTATGC GGGTATCAGA
    AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTATC AGAGAGGAGG TGAAGCTGTG
    AGCAGTGGAG GGAGGCCAGG ACTTCTGAAC ATCAGCGAAC CTGCCACACA GCCCTGGCTG
    GCAGACACGT GGCCCAATAC TGGCAACAAT CACAATGACT GCTCTATCAA TTGCTGTACG
    GCAGGCAATG GTAACAGTGA TAACAACCTG ACCACCTACA GTCGCCCAGC CGATTGTATA
    GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT
    GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA
    AATCTGAAGG ATGGGCGTTT TGTCAATCCA TCAGGGCAGC CCACTCCTTA TGCCACCACC
    CAGCTCATCC AGTCAAACCT CAGCAACAAC ATGAACAATG GCAGCGGGGA CTCCAGCGAG
    AAGCACTGGA AACCTCCCGG CCAGCAGAAA CAAGAAGTGC CACCAGTTCA GTATAACATC
    ATGGAGCAAA ACAAACTGAA CAAAGATTAT CGAGCAAATG ACACAGTTCC TCCAACTATC
    CCATACAACC AATCATATGA CCAGAATACA GGAGGATCCT ACAACAGTTC AGACCGGGGC
    AGTAGTACAT CTGGGAGTCA AGGGCACAAG AAAGGGGCAC GGACACCCAA AGTACCAAAA
    CAAGGTGGCA TGAACTGGGC AGACCTGCTT CCTCCTCCCC CAGCGCATCC TCCTCCTCAT
    AGCAATAGTG AAGAGTACAA TATTTCTGTA GATGAAAGCT ATGACCAAGA AATGCCATGT
    CCCGTGCCAC CAGCAAGGAT GTATTTGCAA CAGAATGAAC TAGAAGAGGA GGAAGATGAG
    CGAGGCCCCA CTCCCCCTGT GCGGGGAGCA GCTTCTTCTC CAGCTGCTGT GTCTTATAGC
    CATCAGTCCA CTGCTACTCT GACCCCCTCC CCACAAGAAG AACTCCAGCC CATGTTGCAG
    GACTGCCCGG AGGAGATTGG CCACATGCAG CACCAACCCG ATAGGAGACG GCAGCCTGTG
    AGTCCTCCTC CACCACCAAG ACCGATTTCC CCACCACATA CCTATGGCTA CATTTCAGGA
    CCTCTTGTCT CAGATATGGA TACAGATGCA CCAGAAGAGG AAGAAGATGA AGCAGACATG
    GAAGTGGCCA AGATGCAGAC CAGACGGCTT TTGTTACGTG GCCTTGAGCA GACCCCTGCC
    TCCAGTGTGG GGGACCTTGA AAGCTCTGTC ACTGGGTCCA TGATCAATGG CTGGGGCTCA
    GCCTCAGAGG AGGACAACAT TTCCAGTGGA CGCTCCAGTG TGAGTTCTTC TGATGGCTCC
    TTCTTCACTG ATGCTGATTT TGCCCAGGCA GTGGCAGCAG CTGCAGAATA TGCTGGTCTT
    AAAGTGGCAC GTCGTCAGAT GCAGGATGCT GCTGGTCGCC GACATTTTCA TGCATCCCAG
    TGCCCTCGAC CCACAAGTCC TGTCTCTACA GACAGCAACA TGAGTGCTGC TGTAATGCAG
    AAGGCCAGAC CAGCCAAGAA GCAAAAACAC CAGCCAGGAC ATCTGCGCAG AGAAGTCTAC
    ACAGATGATC TACCACCTCC TCCGGTGCCA CCACCTGCTA TAAAATCACC CACTGTCCAA
    TCCAAGGCAC AGCTGGAGGT GCGACCTGTT ATGGTGCCAA AACTCCCTTC TATAGAAGCA
    AGAACAGACC GATCATCAGA TAGAAAAGTA GGCAGTTACA AGGGGAGAGA AGTACTGGAT
    GGAAGACAGG TTGCTGATAT GAGAACCAAT CCAGGTGATA CCAGAGAAGC ACAGGAACAG
    CAAAATGATG GAAAAGGACG AGTAAACAAG GCAGCAAAAC GAGACCTTCC ACCAGCAAAG
    ACTCACCTCA TCCAAGAGGA TATCCTACCT TACTGTAGAC CTACTTTTCC GACATCAAAT
    AATCCCAGAG ATCCTAGTTC TTCAAGCTCA ATGTCATCAA GAGGATCAGG AAGCAGACAA
    AGAGAACAAG CAAATGTAGG TCGAAGAAAT ATCGCAGAAA TGCAAGTTCT TGGAGGATAT
    GAAAGAGGAG AAGATAATAA TGAAGAATTA GAGGAAACTG AAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965230.1
    CDS367..5214
    Translation

    Target ORF information:

    RefSeq Version XM_014109755.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109755.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCCGCCCG CCTGTACCTC TTCGGATTGC TCTGTCTCTG CTCAGGCTCC 
    CGTCTCCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCTTCAGA CCTAATTGTG
    TCAAAAGGAG AACCTGCAAC TCTGAACTGT AAAGCTGAAG GCCGCCCCAC GCCCACCATT
    GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA
    ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG AAAAAGTAGG
    CCTGATGAAG GGGTCTACGT CTGTGTGGCA CGGAATTACC TCGGAGAGGC TGTGAGCCAT
    AATGCATCGC TGGAGGTGGC TATACTACGG GATGACTTCA GACAAAATCC TTCTGATGTC
    ATGGTGGCAG TGGGGGAACC TGCTGTGATG GAATGCCAAC CCCCACGAGG CCATCCTGAA
    CCCACCATTT CGTGGAAGAA AGATGGCTCT CCACTGGATG ATAAAGATGA AAGAATAACT
    ATACGAGGTG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCTGG CAAATATGTT
    TGTGTTGGTA CCAACATGGT TGGGGAACGT GAGAGTGAAG TAGCAGAGCT AACTGTCTTG
    GAGAGACCAT CATTTGTGAA GAGACCTAGT AACTTGGCTG TAACTGTGGA TGACAGTGCA
    GAATTTAAAT GTGAGGCTCG AGGTGACCCT GTGCCTACGG TACGATGGAG GAAAGACGAT
    GGAGAACTGC CCAAATCCAG ATACGAAATC AGAGATGATC ATACTTTGAA GATTCGGAAA
    GTGATGGCTG GTGACATGGG GTCATATACT TGTGTTGCAG AAAATATGGT TGGAAAAGCT
    GAAGCATCAG CTACTCTGAC TGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA
    CCTCGTGACC AGGTTGTTGC CTTGGGGCGA ACTGTAACAT TTCAGTGTGA AGCAACCGGA
    AATCCTCAAC CAGCTATTTT TTGGAGAAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT
    CAGCCGCCAC AGTCAACTAG CCGCTTTTCC GTCTCCCAGA CTGGTGATCT CACTATTACT
    AATGTCCAGC GGTCTGATGT TGGTTATTAT ATCTGCCAGA CTTTAAATGT TGCCGGAAGT
    ATCATCACAA AAGCATATTT GGAAGTTACT GATGTGATTG CAGACCGGCC TCCCCCAGTC
    ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT
    GTGGCCACAG GCAGTCCAAT GCCCACGATT CTCTGGAGAA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTAGAATCAA ACAGCTGGAG ACGGGAGTGC TGCAGATCCG ATACGCTAAG
    CTGGGTGACA CAGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA AGCAACATGG
    AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCACG ACCCACCGAC
    CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CAGAAATACA
    GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCAACATC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACA
    GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA
    GCAAATGCCT ATGGAATTAG TGATCCAAGC CAGATATCAG ATCCAGTGAA AACACAAGAT
    GTTCCCCCAA CAAGTCAGGG AGTAGATCAC AAGCAGGTCC AGAGAGAATT GGGAAACGTC
    GTACTGCACC TTCACAACCC CACCATCCTC TCATCCTCTT CCATCGAAGT GCACTGGACA
    GTGGACCAAC AATCTCAGTA TATTCAAGGA TATAAAATTC TTTATCGGCC ATCTGGAGGC
    AACCATGGTG AATCGGAGTG GTTAGTTTTT GAAGTGAGGA CTCCAACCAA AAATAGCGTG
    GTTATCCCTG ATCTCAAAAA GGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT
    GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCTTAGAAGA AGCACCCAGT
    GCCCCACCCC AAAGTGTAAC CGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT
    AGTTGGCAGC CACCTCCAGA AGACACTCAA AATGGAATGG TCCAAGAGTA TAAGGTTTGG
    TGTCTGGGCA ATGAAACTCG TTATCACATA AACAAGACGG TGGATGGTTC CACCTTTTCT
    GTGGTCATTC CTTCTCTGGT TCCTGGGATC CGATACAGCG TAGAAGTAGC AGCCAGCACG
    GGAGCTGGAG CTGGGGTGAA GAGCGAGCCA CAGTTCATCC AGTTAGATTC TCATGGAAAC
    CCCGTGTCAC CTGAAGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGATGT TGTGAAGCAG
    CCAGCGTTCA TTGCGGGAAT CGGGGCCGCC TGCTGGATCA TCCTCATGGT CTTTAGCATC
    TGGCTCTATC GACACCGGAA GAAGAGAAAT GGACTGACTA GTACTTATGC GGGTATCAGA
    AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTATC AGAGAGGAGG TGAAGCTGTG
    AGCAGTGGAG GGAGGCCAGG ACTTCTGAAC ATCAGCGAAC CTGCCACACA GCCCTGGCTG
    GCAGACACGT GGCCCAATAC TGGCAACAAT CACAATGACT GCTCTATCAA TTGCTGTACG
    GCAGGCAATG GTAACAGTGA TAACAACCTG ACCACCTACA GTCGCCCAGC CGATTGTATA
    GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT
    GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA
    AATCTGAAGG ATGGGCGTTT TGTCAATCCA TCAGGGCAGC CCACTCCTTA TGCCACCACC
    CAGCTCATCC AGTCAAACCT CAGCAACAAC ATGAACAATG GCAGCGGGGA CTCCAGCGAG
    AAGCACTGGA AACCTCCCGG CCAGCAGAAA CAAGAAGTGC CACCAGTTCA GTATAACATC
    ATGGAGCAAA ACAAACTGAA CAAAGATTAT CGAGCAAATG ACACAGTTCC TCCAACTATC
    CCATACAACC AATCATATGA CCAGAATACA GGAGGATCCT ACAACAGTTC AGACCGGGGC
    AGTAGTACAT CTGGGAGTCA AGGGCACAAG AAAGGGGCAC GGACACCCAA AGTACCAAAA
    CAAGGTGGCA TGAACTGGGC AGACCTGCTT CCTCCTCCCC CAGCGCATCC TCCTCCTCAT
    AGCAATAGTG AAGAGTACAA TATTTCTGTA GATGAAAGCT ATGACCAAGA AATGCCATGT
    CCCGTGCCAC CAGCAAGGAT GTATTTGCAA CAGAATGAAC TAGAAGAGGA GGAAGATGAG
    CGAGGCCCCA CTCCCCCTGT GCGGGGAGCA GCTTCTTCTC CAGCTGCTGT GTCTTATAGC
    CATCAGTCCA CTGCTACTCT GACCCCCTCC CCACAAGAAG AACTCCAGCC CATGTTGCAG
    GACTGCCCGG AGGAGATTGG CCACATGCAG CACCAACCCG ATAGGAGACG GCAGCCTGTG
    AGTCCTCCTC CACCACCAAG ACCGATTTCC CCACCACATA CCTATGGCTA CATTTCAGGA
    CCTCTTGTCT CAGATATGGA TACAGATGCA CCAGAAGAGG AAGAAGATGA AGCAGACATG
    GAAGTGGCCA AGATGCAGAC CAGACGGCTT TTGTTACGTG GCCTTGAGCA GACCCCTGCC
    TCCAGTGTGG GGGACCTTGA AAGCTCTGTC ACTGGGTCCA TGATCAATGG CTGGGGCTCA
    GCCTCAGAGG AGGACAACAT TTCCAGTGGA CGCTCCAGTG TGAGTTCTTC TGATGGCTCC
    TTCTTCACTG ATGCTGATTT TGCCCAGGCA GTGGCAGCAG CTGCAGAATA TGCTGGTCTT
    AAAGTGGCAC GTCGTCAGAT GCAGGATGCT GCTGGTCGCC GACATTTTCA TGCATCCCAG
    TGCCCTCGAC CCACAAGTCC TGTCTCTACA GACAGCAACA TGAGTGCTGC TGTAATGCAG
    AAGGCCAGAC CAGCCAAGAA GCAAAAACAC CAGCCAGGAC ATCTGCGCAG AGAAGTCTAC
    ACAGATGATC TACCACCTCC TCCGGTGCCA CCACCTGCTA TAAAATCACC CACTGTCCAA
    TCCAAGGCAC AGCTGGAGGT GCGACCTGTT ATGGTGCCAA AACTCCCTTC TATAGAAGCA
    AGAACAGACC GATCATCAGA TAGAAAAGTA GGCAGTTACA AGGGGAGAGA AGTACTGGAT
    GGAAGACAGG TTGCTGATAT GAGAACCAAT CCAGGTGATA CCAGAGAAGC ACAGGAACAG
    CAAAATGATG GAAAAGGACG AGTAAACAAG GCAGCAAAAC GAGACCTTCC ACCAGCAAAG
    ACTCACCTCA TCCAAGAGGA TATCCTACCT TACTGTAGAC CTACTTTTCC GACATCAAAT
    AATCCCAGAG ATCCTAGTTC TTCAAGCTCA ATGTCATCAA GAGGATCAGG AAGCAGACAA
    AGAGAACAAG CAAATGTAGG TCGAAGAAAT ATCGCAGAAA TGCAAGTTCT TGGAGGATAT
    GAAAGAGGAG AAGATAATAA TGAAGAATTA GAGGAAACTG AAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44469
    Clone ID Related Accession (Same CDS sequence) XM_014109756.1
    Accession Version XM_014109756.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4773bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product roundabout homolog 1 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAAATGGA AACATGTTCC TTTTTTGGTC ATAATATCAC TCCTCAGCTT GTCCAAAGAT 
    CACCTGTTCC TGGCGCAGCT TATTCCCGAC CCTGAAGACC TAGAGAGGGG GAACGACAAC
    GGGACGCCAA TCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTCCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CTTCAGACCT AATTGTGTCA
    AAAGGAGAAC CTGCAACTCT GAACTGTAAA GCTGAAGGCC GCCCCACGCC CACCATTGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTGGCACGG AATTACCTCG GAGAGGCTGT GAGCCATAAT
    GCATCGCTGG AGGTGGCTAT ACTACGGGAT GACTTCAGAC AAAATCCTTC TGATGTCATG
    GTGGCAGTGG GGGAACCTGC TGTGATGGAA TGCCAACCCC CACGAGGCCA TCCTGAACCC
    ACCATTTCGT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGTGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCTGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAACGTGAG AGTGAAGTAG CAGAGCTAAC TGTCTTGGAG
    AGACCATCAT TTGTGAAGAG ACCTAGTAAC TTGGCTGTAA CTGTGGATGA CAGTGCAGAA
    TTTAAATGTG AGGCTCGAGG TGACCCTGTG CCTACGGTAC GATGGAGGAA AGACGATGGA
    GAACTGCCCA AATCCAGATA CGAAATCAGA GATGATCATA CTTTGAAGAT TCGGAAAGTG
    ATGGCTGGTG ACATGGGGTC ATATACTTGT GTTGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCAGCTA CTCTGACTGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCT
    CGTGACCAGG TTGTTGCCTT GGGGCGAACT GTAACATTTC AGTGTGAAGC AACCGGAAAT
    CCTCAACCAG CTATTTTTTG GAGAAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCGCCACAGT CAACTAGCCG CTTTTCCGTC TCCCAGACTG GTGATCTCAC TATTACTAAT
    GTCCAGCGGT CTGATGTTGG TTATTATATC TGCCAGACTT TAAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAATGCC CACGATTCTC TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTA GAATCAAACA GCTGGAGACG GGAGTGCTGC AGATCCGATA CGCTAAGCTG
    GGTGACACAG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCACGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCAG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CAACATCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCA
    AATGCCTATG GAATTAGTGA TCCAAGCCAG ATATCAGATC CAGTGAAAAC ACAAGATGTT
    CCCCCAACAA GTCAGGGAGT AGATCACAAG CAGGTCCAGA GAGAATTGGG AAACGTCGTA
    CTGCACCTTC ACAACCCCAC CATCCTCTCA TCCTCTTCCA TCGAAGTGCA CTGGACAGTG
    GACCAACAAT CTCAGTATAT TCAAGGATAT AAAATTCTTT ATCGGCCATC TGGAGGCAAC
    CATGGTGAAT CGGAGTGGTT AGTTTTTGAA GTGAGGACTC CAACCAAAAA TAGCGTGGTT
    ATCCCTGATC TCAAAAAGGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCT TAGAAGAAGC ACCCAGTGCC
    CCACCCCAAA GTGTAACCGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT
    CTGGGCAATG AAACTCGTTA TCACATAAAC AAGACGGTGG ATGGTTCCAC CTTTTCTGTG
    GTCATTCCTT CTCTGGTTCC TGGGATCCGA TACAGCGTAG AAGTAGCAGC CAGCACGGGA
    GCTGGAGCTG GGGTGAAGAG CGAGCCACAG TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AAGACCAAGT CAGCCTTGCT CAGCAGATCT CAGATGTTGT GAAGCAGCCA
    GCGTTCATTG CGGGAATCGG GGCCGCCTGC TGGATCATCC TCATGGTCTT TAGCATCTGG
    CTCTATCGAC ACCGGAAGAA GAGAAATGGA CTGACTAGTA CTTATGCGGG TATCAGAAAA
    GTAACTTATC AGAGAGGAGG TGAAGCTGTG AGCAGTGGAG GGAGGCCAGG ACTTCTGAAC
    ATCAGCGAAC CTGCCACACA GCCCTGGCTG GCAGACACGT GGCCCAATAC TGGCAACAAT
    CACAATGACT GCTCTATCAA TTGCTGTACG GCAGGCAATG GTAACAGTGA TAACAACCTG
    ACCACCTACA GTCGCCCAGG GCAGCCCACT CCTTATGCCA CCACCCAGCT CATCCAGTCA
    AACCTCAGCA ACAACATGAA CAATGGCAGC GGGGACTCCA GCGAGAAGCA CTGGAAACCT
    CCCGGCCAGC AGAAACAAGA AGTGCCACCA GTTCAGTATA ACATCATGGA GCAAAACAAA
    CTGAACAAAG ATTATCGAGC AAATGACACA GTTCCTCCAA CTATCCCATA CAACCAATCA
    TATGACCAGA ATACAGGAGG ATCCTACAAC AGTTCAGACC GGGGCAGTAG TACATCTGGG
    AGTCAAGGGC ACAAGAAAGG GGCACGGACA CCCAAAGTAC CAAAACAAGG TGGCATGAAC
    TGGGCAGACC TGCTTCCTCC TCCCCCAGCG CATCCTCCTC CTCATAGCAA TAGTGAAGAG
    TACAATATTT CTGTAGATGA AAGCTATGAC CAAGAAATGC CATGTCCCGT GCCACCAGCA
    AGGATGTATT TGCAACAGAA TGAACTAGAA GAGGAGGAAG ATGAGCGAGG CCCCACTCCC
    CCTGTGCGGG GAGCAGCTTC TTCTCCAGCT GCTGTGTCTT ATAGCCATCA GTCCACTGCT
    ACTCTGACCC CCTCCCCACA AGAAGAACTC CAGCCCATGT TGCAGGACTG CCCGGAGGAG
    ATTGGCCACA TGCAGCACCA ACCCGATAGG AGACGGCAGC CTGTGAGTCC TCCTCCACCA
    CCAAGACCGA TTTCCCCACC ACATACCTAT GGCTACATTT CAGGACCTCT TGTCTCAGAT
    ATGGATACAG ATGCACCAGA AGAGGAAGAA GATGAAGCAG ACATGGAAGT GGCCAAGATG
    CAGACCAGAC GGCTTTTGTT ACGTGGCCTT GAGCAGACCC CTGCCTCCAG TGTGGGGGAC
    CTTGAAAGCT CTGTCACTGG GTCCATGATC AATGGCTGGG GCTCAGCCTC AGAGGAGGAC
    AACATTTCCA GTGGACGCTC CAGTGTGAGT TCTTCTGATG GCTCCTTCTT CACTGATGCT
    GATTTTGCCC AGGCAGTGGC AGCAGCTGCA GAATATGCTG GTCTTAAAGT GGCACGTCGT
    CAGATGCAGG ATGCTGCTGG TCGCCGACAT TTTCATGCAT CCCAGTGCCC TCGACCCACA
    AGTCCTGTCT CTACAGACAG CAACATGAGT GCTGCTGTAA TGCAGAAGGC CAGACCAGCC
    AAGAAGCAAA AACACCAGCC AGGACATCTG CGCAGAGAAG TCTACACAGA TGATCTACCA
    CCTCCTCCGG TGCCACCACC TGCTATAAAA TCACCCACTG TCCAATCCAA GGCACAGCTG
    GAGGTGCGAC CTGTTATGGT GCCAAAACTC CCTTCTATAG AAGCAAGAAC AGACCGATCA
    TCAGATAGAA AAGTAGGCAG TTACAAGGGG AGAGAAGTAC TGGATGGAAG ACAGGTTGCT
    GATATGAGAA CCAATCCAGG TGATACCAGA GAAGCACAGG AACAGCAAAA TGATGGAAAA
    GGACGAGTAA ACAAGGCAGC AAAACGAGAC CTTCCACCAG CAAAGACTCA CCTCATCCAA
    GAGGATATCC TACCTTACTG TAGACCTACT TTTCCGACAT CAAATAATCC CAGAGATCCT
    AGTTCTTCAA GCTCAATGTC ATCAAGAGGA TCAGGAAGCA GACAAAGAGA ACAAGCAAAT
    GTAGGTCGAA GAAATATCGC AGAAATGCAA GTTCTTGGAG GATATGAAAG AGGAGAAGAT
    AATAATGAAG AATTAGAGGA AACTGAAAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965231.1
    CDS303..5075
    Translation

    Target ORF information:

    RefSeq Version XM_014109756.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109756.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAAATGGA AACATGTTCC TTTTTTGGTC ATAATATCAC TCCTCAGCTT GTCCAAAGAT 
    CACCTGTTCC TGGCGCAGCT TATTCCCGAC CCTGAAGACC TAGAGAGGGG GAACGACAAC
    GGGACGCCAA TCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTCCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CTTCAGACCT AATTGTGTCA
    AAAGGAGAAC CTGCAACTCT GAACTGTAAA GCTGAAGGCC GCCCCACGCC CACCATTGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTGGCACGG AATTACCTCG GAGAGGCTGT GAGCCATAAT
    GCATCGCTGG AGGTGGCTAT ACTACGGGAT GACTTCAGAC AAAATCCTTC TGATGTCATG
    GTGGCAGTGG GGGAACCTGC TGTGATGGAA TGCCAACCCC CACGAGGCCA TCCTGAACCC
    ACCATTTCGT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGTGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCTGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAACGTGAG AGTGAAGTAG CAGAGCTAAC TGTCTTGGAG
    AGACCATCAT TTGTGAAGAG ACCTAGTAAC TTGGCTGTAA CTGTGGATGA CAGTGCAGAA
    TTTAAATGTG AGGCTCGAGG TGACCCTGTG CCTACGGTAC GATGGAGGAA AGACGATGGA
    GAACTGCCCA AATCCAGATA CGAAATCAGA GATGATCATA CTTTGAAGAT TCGGAAAGTG
    ATGGCTGGTG ACATGGGGTC ATATACTTGT GTTGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCAGCTA CTCTGACTGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCT
    CGTGACCAGG TTGTTGCCTT GGGGCGAACT GTAACATTTC AGTGTGAAGC AACCGGAAAT
    CCTCAACCAG CTATTTTTTG GAGAAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCGCCACAGT CAACTAGCCG CTTTTCCGTC TCCCAGACTG GTGATCTCAC TATTACTAAT
    GTCCAGCGGT CTGATGTTGG TTATTATATC TGCCAGACTT TAAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAATGCC CACGATTCTC TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTA GAATCAAACA GCTGGAGACG GGAGTGCTGC AGATCCGATA CGCTAAGCTG
    GGTGACACAG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCACGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCAG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CAACATCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCA
    AATGCCTATG GAATTAGTGA TCCAAGCCAG ATATCAGATC CAGTGAAAAC ACAAGATGTT
    CCCCCAACAA GTCAGGGAGT AGATCACAAG CAGGTCCAGA GAGAATTGGG AAACGTCGTA
    CTGCACCTTC ACAACCCCAC CATCCTCTCA TCCTCTTCCA TCGAAGTGCA CTGGACAGTG
    GACCAACAAT CTCAGTATAT TCAAGGATAT AAAATTCTTT ATCGGCCATC TGGAGGCAAC
    CATGGTGAAT CGGAGTGGTT AGTTTTTGAA GTGAGGACTC CAACCAAAAA TAGCGTGGTT
    ATCCCTGATC TCAAAAAGGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCT TAGAAGAAGC ACCCAGTGCC
    CCACCCCAAA GTGTAACCGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT
    CTGGGCAATG AAACTCGTTA TCACATAAAC AAGACGGTGG ATGGTTCCAC CTTTTCTGTG
    GTCATTCCTT CTCTGGTTCC TGGGATCCGA TACAGCGTAG AAGTAGCAGC CAGCACGGGA
    GCTGGAGCTG GGGTGAAGAG CGAGCCACAG TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AAGACCAAGT CAGCCTTGCT CAGCAGATCT CAGATGTTGT GAAGCAGCCA
    GCGTTCATTG CGGGAATCGG GGCCGCCTGC TGGATCATCC TCATGGTCTT TAGCATCTGG
    CTCTATCGAC ACCGGAAGAA GAGAAATGGA CTGACTAGTA CTTATGCGGG TATCAGAAAA
    GTAACTTATC AGAGAGGAGG TGAAGCTGTG AGCAGTGGAG GGAGGCCAGG ACTTCTGAAC
    ATCAGCGAAC CTGCCACACA GCCCTGGCTG GCAGACACGT GGCCCAATAC TGGCAACAAT
    CACAATGACT GCTCTATCAA TTGCTGTACG GCAGGCAATG GTAACAGTGA TAACAACCTG
    ACCACCTACA GTCGCCCAGG GCAGCCCACT CCTTATGCCA CCACCCAGCT CATCCAGTCA
    AACCTCAGCA ACAACATGAA CAATGGCAGC GGGGACTCCA GCGAGAAGCA CTGGAAACCT
    CCCGGCCAGC AGAAACAAGA AGTGCCACCA GTTCAGTATA ACATCATGGA GCAAAACAAA
    CTGAACAAAG ATTATCGAGC AAATGACACA GTTCCTCCAA CTATCCCATA CAACCAATCA
    TATGACCAGA ATACAGGAGG ATCCTACAAC AGTTCAGACC GGGGCAGTAG TACATCTGGG
    AGTCAAGGGC ACAAGAAAGG GGCACGGACA CCCAAAGTAC CAAAACAAGG TGGCATGAAC
    TGGGCAGACC TGCTTCCTCC TCCCCCAGCG CATCCTCCTC CTCATAGCAA TAGTGAAGAG
    TACAATATTT CTGTAGATGA AAGCTATGAC CAAGAAATGC CATGTCCCGT GCCACCAGCA
    AGGATGTATT TGCAACAGAA TGAACTAGAA GAGGAGGAAG ATGAGCGAGG CCCCACTCCC
    CCTGTGCGGG GAGCAGCTTC TTCTCCAGCT GCTGTGTCTT ATAGCCATCA GTCCACTGCT
    ACTCTGACCC CCTCCCCACA AGAAGAACTC CAGCCCATGT TGCAGGACTG CCCGGAGGAG
    ATTGGCCACA TGCAGCACCA ACCCGATAGG AGACGGCAGC CTGTGAGTCC TCCTCCACCA
    CCAAGACCGA TTTCCCCACC ACATACCTAT GGCTACATTT CAGGACCTCT TGTCTCAGAT
    ATGGATACAG ATGCACCAGA AGAGGAAGAA GATGAAGCAG ACATGGAAGT GGCCAAGATG
    CAGACCAGAC GGCTTTTGTT ACGTGGCCTT GAGCAGACCC CTGCCTCCAG TGTGGGGGAC
    CTTGAAAGCT CTGTCACTGG GTCCATGATC AATGGCTGGG GCTCAGCCTC AGAGGAGGAC
    AACATTTCCA GTGGACGCTC CAGTGTGAGT TCTTCTGATG GCTCCTTCTT CACTGATGCT
    GATTTTGCCC AGGCAGTGGC AGCAGCTGCA GAATATGCTG GTCTTAAAGT GGCACGTCGT
    CAGATGCAGG ATGCTGCTGG TCGCCGACAT TTTCATGCAT CCCAGTGCCC TCGACCCACA
    AGTCCTGTCT CTACAGACAG CAACATGAGT GCTGCTGTAA TGCAGAAGGC CAGACCAGCC
    AAGAAGCAAA AACACCAGCC AGGACATCTG CGCAGAGAAG TCTACACAGA TGATCTACCA
    CCTCCTCCGG TGCCACCACC TGCTATAAAA TCACCCACTG TCCAATCCAA GGCACAGCTG
    GAGGTGCGAC CTGTTATGGT GCCAAAACTC CCTTCTATAG AAGCAAGAAC AGACCGATCA
    TCAGATAGAA AAGTAGGCAG TTACAAGGGG AGAGAAGTAC TGGATGGAAG ACAGGTTGCT
    GATATGAGAA CCAATCCAGG TGATACCAGA GAAGCACAGG AACAGCAAAA TGATGGAAAA
    GGACGAGTAA ACAAGGCAGC AAAACGAGAC CTTCCACCAG CAAAGACTCA CCTCATCCAA
    GAGGATATCC TACCTTACTG TAGACCTACT TTTCCGACAT CAAATAATCC CAGAGATCCT
    AGTTCTTCAA GCTCAATGTC ATCAAGAGGA TCAGGAAGCA GACAAAGAGA ACAAGCAAAT
    GTAGGTCGAA GAAATATCGC AGAAATGCAA GTTCTTGGAG GATATGAAAG AGGAGAAGAT
    AATAATGAAG AATTAGAGGA AACTGAAAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29277
    Clone ID Related Accession (Same CDS sequence) XM_544814.5
    Accession Version XM_544814.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product roundabout homolog 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_544814.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA AACATGTTCC TTTTTTGGTC ATAATATCAC TCCTCAGCTT GTCCAAAGAT 
    CACCTGTTCC TGGCGCAGCT TATTCCCGAC CCTGAAGACC TAGAGAGGGG GAACGACAAC
    GGGACGCCAA TCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTCCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CTTCAGACCT AATTGTGTCA
    AAAGGAGAAC CTGCAACTCT GAACTGTAAA GCTGAAGGCC GCCCCACGCC CACCATTGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTGGCACGG AATTACCTCG GAGAGGCTGT GAGCCATAAT
    GCATCGCTGG AGGTGGCTAT ACTACGGGAT GACTTCAGAC AAAATCCTTC TGATGTCATG
    GTGGCAGTGG GGGAACCTGC TGTGATGGAA TGCCAACCCC CACGAGGCCA TCCTGAACCC
    ACCATTTCGT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGTGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCTGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAACGTGAG AGTGAAGTAG CAGAGCTAAC TGTCTTGGAG
    AGACCATCAT TTGTGAAGAG ACCTAGTAAC TTGGCTGTAA CTGTGGATGA CAGTGCAGAA
    TTTAAATGTG AGGCTCGAGG TGACCCTGTG CCTACGGTAC GATGGAGGAA AGACGATGGA
    GAACTGCCCA AATCCAGATA CGAAATCAGA GATGATCATA CTTTGAAGAT TCGGAAAGTG
    ATGGCTGGTG ACATGGGGTC ATATACTTGT GTTGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCAGCTA CTCTGACTGT CCAAGAACCT CCACATTTTG TTGTGAAACC TCGTGACCAG
    GTTGTTGCCT TGGGGCGAAC TGTAACATTT CAGTGTGAAG CAACCGGAAA TCCTCAACCA
    GCTATTTTTT GGAGAAGAGA GGGGAGTCAG AATCTGCTTT TCTCATATCA GCCGCCACAG
    TCAACTAGCC GCTTTTCCGT CTCCCAGACT GGTGATCTCA CTATTACTAA TGTCCAGCGG
    TCTGATGTTG GTTATTATAT CTGCCAGACT TTAAATGTTG CCGGAAGTAT CATCACAAAA
    GCATATTTGG AAGTTACTGA TGTGATTGCA GACCGGCCTC CCCCAGTCAT TCGACAAGGT
    CCTGTGAATC AGACTGTAGC AGTGGATGGT ACTTTAGTGC TCAGCTGTGT GGCCACAGGC
    AGTCCAATGC CCACGATTCT CTGGAGAAAG GATGGAGTCC TTGTTTCAAC CCAAGATTCT
    AGAATCAAAC AGCTGGAGAC GGGAGTGCTG CAGATCCGAT ACGCTAAGCT GGGTGACACA
    GGTCGGTACA CCTGCATTGC ATCAACCCCC AGTGGTGAAG CAACATGGAG TGCTTACATT
    GAAGTCCAAG AATTTGGAGT TCCAGTTCAG CCTCCACGAC CCACCGACCC AAATTTAATC
    CCTAGTGCCC CCTCAAAACC TGAAGTTACA GATGTCAGCA GAAATACAGT AACGTTGTCA
    TGGCAACCAA ATTTGAATTC AGGTGCAACT CCAACATCTT ATATTATAGA AGCCTTCAGC
    CATGCATCTG GTAGCAGCTG GCAGACCGTA GCAGAGAATG TGAAAACAGA AACATTTGCC
    ATTAAAGGAC TCAAACCTAA TGCAATTTAC CTTTTCCTTG TGAGGGCAGC AAATGCCTAT
    GGAATTAGTG ATCCAAGCCA GATATCAGAT CCAGTGAAAA CACAAGATGT TCCCCCAACA
    AGTCAGGGAG TAGATCACAA GCAGGTCCAG AGAGAATTGG GAAACGTCGT ACTGCACCTT
    CACAACCCCA CCATCCTCTC ATCCTCTTCC ATCGAAGTGC ACTGGACAGT GGACCAACAA
    TCTCAGTATA TTCAAGGATA TAAAATTCTT TATCGGCCAT CTGGAGGCAA CCATGGTGAA
    TCGGAGTGGT TAGTTTTTGA AGTGAGGACT CCAACCAAAA ATAGCGTGGT TATCCCTGAT
    CTCAAAAAGG GAGTCAACTA TGAAATTAAG GCTCGTCCTT TTTTTAATGA ATTTCAAGGA
    GCAGATAGTG AAATTAAATT TGCCAAAACC TTAGAAGAAG CACCCAGTGC CCCACCCCAA
    AGTGTAACCG TATCAAAGAA TGATGGAAAT GGAACTGCAA TTCTTGTTAG TTGGCAGCCA
    CCTCCAGAAG ACACTCAAAA TGGAATGGTC CAAGAGTATA AGGTTTGGTG TCTGGGCAAT
    GAAACTCGTT ATCACATAAA CAAGACGGTG GATGGTTCCA CCTTTTCTGT GGTCATTCCT
    TCTCTGGTTC CTGGGATCCG ATACAGCGTA GAAGTAGCAG CCAGCACGGG AGCTGGAGCT
    GGGGTGAAGA GCGAGCCACA GTTCATCCAG TTAGATTCTC ATGGAAACCC CGTGTCACCT
    GAAGACCAAG TCAGCCTTGC TCAGCAGATC TCAGATGTTG TGAAGCAGCC AGCGTTCATT
    GCGGGAATCG GGGCCGCCTG CTGGATCATC CTCATGGTCT TTAGCATCTG GCTCTATCGA
    CACCGGAAGA AGAGAAATGG ACTGACTAGT ACTTATGCGG GTATCAGAAA AGTCCCGTCT
    TTTACCTTCA CACCAACAGT AACTTATCAG AGAGGAGGTG AAGCTGTGAG CAGTGGAGGG
    AGGCCAGGAC TTCTGAACAT CAGCGAACCT GCCACACAGC CCTGGCTGGC AGACACGTGG
    CCCAATACTG GCAACAATCA CAATGACTGC TCTATCAATT GCTGTACGGC AGGCAATGGT
    AACAGTGATA ACAACCTGAC CACCTACAGT CGCCCAGCCG ATTGTATAGC AAATTATAAC
    AACCAACTGG ATAACAAACA AACAAATCTG ATGCTCCCTG AGTCAACTGT TTATGGTGAT
    GTGGACCTTA GTAACAAAAT CAATGAGATG AAAACCTTCA ATAGCCCAAA TCTGAAGGAT
    GGGCGTTTTG TCAATCCATC AGGGCAGCCC ACTCCTTATG CCACCACCCA GCTCATCCAG
    TCAAACCTCA GCAACAACAT GAACAATGGC AGCGGGGACT CCAGCGAGAA GCACTGGAAA
    CCTCCCGGCC AGCAGAAACA AGAAGTGCCA CCAGTTCAGT ATAACATCAT GGAGCAAAAC
    AAACTGAACA AAGATTATCG AGCAAATGAC ACAGTTCCTC CAACTATCCC ATACAACCAA
    TCATATGACC AGAATACAGG AGGATCCTAC AACAGTTCAG ACCGGGGCAG TAGTACATCT
    GGGAGTCAAG GGCACAAGAA AGGGGCACGG ACACCCAAAG TACCAAAACA AGGTGGCATG
    AACTGGGCAG ACCTGCTTCC TCCTCCCCCA GCGCATCCTC CTCCTCATAG CAATAGTGAA
    GAGTACAATA TTTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGTCC CGTGCCACCA
    GCAAGGATGT ATTTGCAACA GAATGAACTA GAAGAGGAGG AAGATGAGCG AGGCCCCACT
    CCCCCTGTGC GGGGAGCAGC TTCTTCTCCA GCTGCTGTGT CTTATAGCCA TCAGTCCACT
    GCTACTCTGA CCCCCTCCCC ACAAGAAGAA CTCCAGCCCA TGTTGCAGGA CTGCCCGGAG
    GAGATTGGCC ACATGCAGCA CCAACCCGAT AGGAGACGGC AGCCTGTGAG TCCTCCTCCA
    CCACCAAGAC CGATTTCCCC ACCACATACC TATGGCTACA TTTCAGGACC TCTTGTCTCA
    GATATGGATA CAGATGCACC AGAAGAGGAA GAAGATGAAG CAGACATGGA AGTGGCCAAG
    ATGCAGACCA GACGGCTTTT GTTACGTGGC CTTGAGCAGA CCCCTGCCTC CAGTGTGGGG
    GACCTTGAAA GCTCTGTCAC TGGGTCCATG ATCAATGGCT GGGGCTCAGC CTCAGAGGAG
    GACAACATTT CCAGTGGACG CTCCAGTGTG AGTTCTTCTG ATGGCTCCTT CTTCACTGAT
    GCTGATTTTG CCCAGGCAGT GGCAGCAGCT GCAGAATATG CTGGTCTTAA AGTGGCACGT
    CGTCAGATGC AGGATGCTGC TGGTCGCCGA CATTTTCATG CATCCCAGTG CCCTCGACCC
    ACAAGTCCTG TCTCTACAGA CAGCAACATG AGTGCTGCTG TAATGCAGAA GGCCAGACCA
    GCCAAGAAGC AAAAACACCA GCCAGGACAT CTGCGCAGAG AAGTCTACAC AGATGATCTA
    CCACCTCCTC CGGTGCCACC ACCTGCTATA AAATCACCCA CTGTCCAATC CAAGGCACAG
    CTGGAGGTGC GACCTGTTAT GGTGCCAAAA CTCCCTTCTA TAGAAGCAAG AACAGACCGA
    TCATCAGATA GAAAAGTAGG CAGTTACAAG GGGAGAGAAG TACTGGATGG AAGACAGGTT
    GCTGATATGA GAACCAATCC AGGTGATACC AGAGAAGCAC AGGAACAGCA AAATGATGGA
    AAAGGACGAG TAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGAC TCACCTCATC
    CAAGAGGATA TCCTACCTTA CTGTAGACCT ACTTTTCCGA CATCAAATAA TCCCAGAGAT
    CCTAGTTCTT CAAGCTCAAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA
    AATGTAGGTC GAAGAAATAT CGCAGAAATG CAAGTTCTTG GAGGATATGA AAGAGGAGAA
    GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_544814.4
    CDS303..5258
    Translation

    Target ORF information:

    RefSeq Version XM_544814.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_544814.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA AACATGTTCC TTTTTTGGTC ATAATATCAC TCCTCAGCTT GTCCAAAGAT 
    CACCTGTTCC TGGCGCAGCT TATTCCCGAC CCTGAAGACC TAGAGAGGGG GAACGACAAC
    GGGACGCCAA TCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTCCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CTTCAGACCT AATTGTGTCA
    AAAGGAGAAC CTGCAACTCT GAACTGTAAA GCTGAAGGCC GCCCCACGCC CACCATTGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTGGCACGG AATTACCTCG GAGAGGCTGT GAGCCATAAT
    GCATCGCTGG AGGTGGCTAT ACTACGGGAT GACTTCAGAC AAAATCCTTC TGATGTCATG
    GTGGCAGTGG GGGAACCTGC TGTGATGGAA TGCCAACCCC CACGAGGCCA TCCTGAACCC
    ACCATTTCGT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGTGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCTGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAACGTGAG AGTGAAGTAG CAGAGCTAAC TGTCTTGGAG
    AGACCATCAT TTGTGAAGAG ACCTAGTAAC TTGGCTGTAA CTGTGGATGA CAGTGCAGAA
    TTTAAATGTG AGGCTCGAGG TGACCCTGTG CCTACGGTAC GATGGAGGAA AGACGATGGA
    GAACTGCCCA AATCCAGATA CGAAATCAGA GATGATCATA CTTTGAAGAT TCGGAAAGTG
    ATGGCTGGTG ACATGGGGTC ATATACTTGT GTTGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCAGCTA CTCTGACTGT CCAAGAACCT CCACATTTTG TTGTGAAACC TCGTGACCAG
    GTTGTTGCCT TGGGGCGAAC TGTAACATTT CAGTGTGAAG CAACCGGAAA TCCTCAACCA
    GCTATTTTTT GGAGAAGAGA GGGGAGTCAG AATCTGCTTT TCTCATATCA GCCGCCACAG
    TCAACTAGCC GCTTTTCCGT CTCCCAGACT GGTGATCTCA CTATTACTAA TGTCCAGCGG
    TCTGATGTTG GTTATTATAT CTGCCAGACT TTAAATGTTG CCGGAAGTAT CATCACAAAA
    GCATATTTGG AAGTTACTGA TGTGATTGCA GACCGGCCTC CCCCAGTCAT TCGACAAGGT
    CCTGTGAATC AGACTGTAGC AGTGGATGGT ACTTTAGTGC TCAGCTGTGT GGCCACAGGC
    AGTCCAATGC CCACGATTCT CTGGAGAAAG GATGGAGTCC TTGTTTCAAC CCAAGATTCT
    AGAATCAAAC AGCTGGAGAC GGGAGTGCTG CAGATCCGAT ACGCTAAGCT GGGTGACACA
    GGTCGGTACA CCTGCATTGC ATCAACCCCC AGTGGTGAAG CAACATGGAG TGCTTACATT
    GAAGTCCAAG AATTTGGAGT TCCAGTTCAG CCTCCACGAC CCACCGACCC AAATTTAATC
    CCTAGTGCCC CCTCAAAACC TGAAGTTACA GATGTCAGCA GAAATACAGT AACGTTGTCA
    TGGCAACCAA ATTTGAATTC AGGTGCAACT CCAACATCTT ATATTATAGA AGCCTTCAGC
    CATGCATCTG GTAGCAGCTG GCAGACCGTA GCAGAGAATG TGAAAACAGA AACATTTGCC
    ATTAAAGGAC TCAAACCTAA TGCAATTTAC CTTTTCCTTG TGAGGGCAGC AAATGCCTAT
    GGAATTAGTG ATCCAAGCCA GATATCAGAT CCAGTGAAAA CACAAGATGT TCCCCCAACA
    AGTCAGGGAG TAGATCACAA GCAGGTCCAG AGAGAATTGG GAAACGTCGT ACTGCACCTT
    CACAACCCCA CCATCCTCTC ATCCTCTTCC ATCGAAGTGC ACTGGACAGT GGACCAACAA
    TCTCAGTATA TTCAAGGATA TAAAATTCTT TATCGGCCAT CTGGAGGCAA CCATGGTGAA
    TCGGAGTGGT TAGTTTTTGA AGTGAGGACT CCAACCAAAA ATAGCGTGGT TATCCCTGAT
    CTCAAAAAGG GAGTCAACTA TGAAATTAAG GCTCGTCCTT TTTTTAATGA ATTTCAAGGA
    GCAGATAGTG AAATTAAATT TGCCAAAACC TTAGAAGAAG CACCCAGTGC CCCACCCCAA
    AGTGTAACCG TATCAAAGAA TGATGGAAAT GGAACTGCAA TTCTTGTTAG TTGGCAGCCA
    CCTCCAGAAG ACACTCAAAA TGGAATGGTC CAAGAGTATA AGGTTTGGTG TCTGGGCAAT
    GAAACTCGTT ATCACATAAA CAAGACGGTG GATGGTTCCA CCTTTTCTGT GGTCATTCCT
    TCTCTGGTTC CTGGGATCCG ATACAGCGTA GAAGTAGCAG CCAGCACGGG AGCTGGAGCT
    GGGGTGAAGA GCGAGCCACA GTTCATCCAG TTAGATTCTC ATGGAAACCC CGTGTCACCT
    GAAGACCAAG TCAGCCTTGC TCAGCAGATC TCAGATGTTG TGAAGCAGCC AGCGTTCATT
    GCGGGAATCG GGGCCGCCTG CTGGATCATC CTCATGGTCT TTAGCATCTG GCTCTATCGA
    CACCGGAAGA AGAGAAATGG ACTGACTAGT ACTTATGCGG GTATCAGAAA AGTCCCGTCT
    TTTACCTTCA CACCAACAGT AACTTATCAG AGAGGAGGTG AAGCTGTGAG CAGTGGAGGG
    AGGCCAGGAC TTCTGAACAT CAGCGAACCT GCCACACAGC CCTGGCTGGC AGACACGTGG
    CCCAATACTG GCAACAATCA CAATGACTGC TCTATCAATT GCTGTACGGC AGGCAATGGT
    AACAGTGATA ACAACCTGAC CACCTACAGT CGCCCAGCCG ATTGTATAGC AAATTATAAC
    AACCAACTGG ATAACAAACA AACAAATCTG ATGCTCCCTG AGTCAACTGT TTATGGTGAT
    GTGGACCTTA GTAACAAAAT CAATGAGATG AAAACCTTCA ATAGCCCAAA TCTGAAGGAT
    GGGCGTTTTG TCAATCCATC AGGGCAGCCC ACTCCTTATG CCACCACCCA GCTCATCCAG
    TCAAACCTCA GCAACAACAT GAACAATGGC AGCGGGGACT CCAGCGAGAA GCACTGGAAA
    CCTCCCGGCC AGCAGAAACA AGAAGTGCCA CCAGTTCAGT ATAACATCAT GGAGCAAAAC
    AAACTGAACA AAGATTATCG AGCAAATGAC ACAGTTCCTC CAACTATCCC ATACAACCAA
    TCATATGACC AGAATACAGG AGGATCCTAC AACAGTTCAG ACCGGGGCAG TAGTACATCT
    GGGAGTCAAG GGCACAAGAA AGGGGCACGG ACACCCAAAG TACCAAAACA AGGTGGCATG
    AACTGGGCAG ACCTGCTTCC TCCTCCCCCA GCGCATCCTC CTCCTCATAG CAATAGTGAA
    GAGTACAATA TTTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGTCC CGTGCCACCA
    GCAAGGATGT ATTTGCAACA GAATGAACTA GAAGAGGAGG AAGATGAGCG AGGCCCCACT
    CCCCCTGTGC GGGGAGCAGC TTCTTCTCCA GCTGCTGTGT CTTATAGCCA TCAGTCCACT
    GCTACTCTGA CCCCCTCCCC ACAAGAAGAA CTCCAGCCCA TGTTGCAGGA CTGCCCGGAG
    GAGATTGGCC ACATGCAGCA CCAACCCGAT AGGAGACGGC AGCCTGTGAG TCCTCCTCCA
    CCACCAAGAC CGATTTCCCC ACCACATACC TATGGCTACA TTTCAGGACC TCTTGTCTCA
    GATATGGATA CAGATGCACC AGAAGAGGAA GAAGATGAAG CAGACATGGA AGTGGCCAAG
    ATGCAGACCA GACGGCTTTT GTTACGTGGC CTTGAGCAGA CCCCTGCCTC CAGTGTGGGG
    GACCTTGAAA GCTCTGTCAC TGGGTCCATG ATCAATGGCT GGGGCTCAGC CTCAGAGGAG
    GACAACATTT CCAGTGGACG CTCCAGTGTG AGTTCTTCTG ATGGCTCCTT CTTCACTGAT
    GCTGATTTTG CCCAGGCAGT GGCAGCAGCT GCAGAATATG CTGGTCTTAA AGTGGCACGT
    CGTCAGATGC AGGATGCTGC TGGTCGCCGA CATTTTCATG CATCCCAGTG CCCTCGACCC
    ACAAGTCCTG TCTCTACAGA CAGCAACATG AGTGCTGCTG TAATGCAGAA GGCCAGACCA
    GCCAAGAAGC AAAAACACCA GCCAGGACAT CTGCGCAGAG AAGTCTACAC AGATGATCTA
    CCACCTCCTC CGGTGCCACC ACCTGCTATA AAATCACCCA CTGTCCAATC CAAGGCACAG
    CTGGAGGTGC GACCTGTTAT GGTGCCAAAA CTCCCTTCTA TAGAAGCAAG AACAGACCGA
    TCATCAGATA GAAAAGTAGG CAGTTACAAG GGGAGAGAAG TACTGGATGG AAGACAGGTT
    GCTGATATGA GAACCAATCC AGGTGATACC AGAGAAGCAC AGGAACAGCA AAATGATGGA
    AAAGGACGAG TAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGAC TCACCTCATC
    CAAGAGGATA TCCTACCTTA CTGTAGACCT ACTTTTCCGA CATCAAATAA TCCCAGAGAT
    CCTAGTTCTT CAAGCTCAAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA
    AATGTAGGTC GAAGAAATAT CGCAGAAATG CAAGTTCTTG GAGGATATGA AAGAGGAGAA
    GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29280
    Clone ID Related Accession (Same CDS sequence) XM_005638751.2
    Accession Version XM_005638751.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product roundabout homolog 1 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638751.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAAATGGA AACATGTTCC TTTTTTGGTC ATAATATCAC TCCTCAGCTT GTCCAAAGAT 
    CACCTGTTCC TGGCGCAGCT TATTCCCGAC CCTGAAGACC TAGAGAGGGG GAACGACAAC
    GGGACGCCAA TCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTCCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CTTCAGACCT AATTGTGTCA
    AAAGGAGAAC CTGCAACTCT GAACTGTAAA GCTGAAGGCC GCCCCACGCC CACCATTGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTGGCACGG AATTACCTCG GAGAGGCTGT GAGCCATAAT
    GCATCGCTGG AGGTGGCTAT ACTACGGGAT GACTTCAGAC AAAATCCTTC TGATGTCATG
    GTGGCAGTGG GGGAACCTGC TGTGATGGAA TGCCAACCCC CACGAGGCCA TCCTGAACCC
    ACCATTTCGT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGTGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCTGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAACGTGAG AGTGAAGTAG CAGAGCTAAC TGTCTTGGAG
    AGACCATCAT TTGTGAAGAG ACCTAGTAAC TTGGCTGTAA CTGTGGATGA CAGTGCAGAA
    TTTAAATGTG AGGCTCGAGG TGACCCTGTG CCTACGGTAC GATGGAGGAA AGACGATGGA
    GAACTGCCCA AATCCAGATA CGAAATCAGA GATGATCATA CTTTGAAGAT TCGGAAAGTG
    ATGGCTGGTG ACATGGGGTC ATATACTTGT GTTGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCAGCTA CTCTGACTGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCT
    CGTGACCAGG TTGTTGCCTT GGGGCGAACT GTAACATTTC AGTGTGAAGC AACCGGAAAT
    CCTCAACCAG CTATTTTTTG GAGAAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCGCCACAGT CAACTAGCCG CTTTTCCGTC TCCCAGACTG GTGATCTCAC TATTACTAAT
    GTCCAGCGGT CTGATGTTGG TTATTATATC TGCCAGACTT TAAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAATGCC CACGATTCTC TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTA GAATCAAACA GCTGGAGACG GGAGTGCTGC AGATCCGATA CGCTAAGCTG
    GGTGACACAG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCACGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCAG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CAACATCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCA
    AATGCCTATG GAATTAGTGA TCCAAGCCAG ATATCAGATC CAGTGAAAAC ACAAGATGTT
    CCCCCAACAA GTCAGGGAGT AGATCACAAG CAGGTCCAGA GAGAATTGGG AAACGTCGTA
    CTGCACCTTC ACAACCCCAC CATCCTCTCA TCCTCTTCCA TCGAAGTGCA CTGGACAGTG
    GACCAACAAT CTCAGTATAT TCAAGGATAT AAAATTCTTT ATCGGCCATC TGGAGGCAAC
    CATGGTGAAT CGGAGTGGTT AGTTTTTGAA GTGAGGACTC CAACCAAAAA TAGCGTGGTT
    ATCCCTGATC TCAAAAAGGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCT TAGAAGAAGC ACCCAGTGCC
    CCACCCCAAA GTGTAACCGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT
    CTGGGCAATG AAACTCGTTA TCACATAAAC AAGACGGTGG ATGGTTCCAC CTTTTCTGTG
    GTCATTCCTT CTCTGGTTCC TGGGATCCGA TACAGCGTAG AAGTAGCAGC CAGCACGGGA
    GCTGGAGCTG GGGTGAAGAG CGAGCCACAG TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AAGACCAAGT CAGCCTTGCT CAGCAGATCT CAGATGTTGT GAAGCAGCCA
    GCGTTCATTG CGGGAATCGG GGCCGCCTGC TGGATCATCC TCATGGTCTT TAGCATCTGG
    CTCTATCGAC ACCGGAAGAA GAGAAATGGA CTGACTAGTA CTTATGCGGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGTGA AGCTGTGAGC
    AGTGGAGGGA GGCCAGGACT TCTGAACATC AGCGAACCTG CCACACAGCC CTGGCTGGCA
    GACACGTGGC CCAATACTGG CAACAATCAC AATGACTGCT CTATCAATTG CTGTACGGCA
    GGCAATGGTA ACAGTGATAA CAACCTGACC ACCTACAGTC GCCCAGGGCA GCCCACTCCT
    TATGCCACCA CCCAGCTCAT CCAGTCAAAC CTCAGCAACA ACATGAACAA TGGCAGCGGG
    GACTCCAGCG AGAAGCACTG GAAACCTCCC GGCCAGCAGA AACAAGAAGT GCCACCAGTT
    CAGTATAACA TCATGGAGCA AAACAAACTG AACAAAGATT ATCGAGCAAA TGACACAGTT
    CCTCCAACTA TCCCATACAA CCAATCATAT GACCAGAATA CAGGAGGATC CTACAACAGT
    TCAGACCGGG GCAGTAGTAC ATCTGGGAGT CAAGGGCACA AGAAAGGGGC ACGGACACCC
    AAAGTACCAA AACAAGGTGG CATGAACTGG GCAGACCTGC TTCCTCCTCC CCCAGCGCAT
    CCTCCTCCTC ATAGCAATAG TGAAGAGTAC AATATTTCTG TAGATGAAAG CTATGACCAA
    GAAATGCCAT GTCCCGTGCC ACCAGCAAGG ATGTATTTGC AACAGAATGA ACTAGAAGAG
    GAGGAAGATG AGCGAGGCCC CACTCCCCCT GTGCGGGGAG CAGCTTCTTC TCCAGCTGCT
    GTGTCTTATA GCCATCAGTC CACTGCTACT CTGACCCCCT CCCCACAAGA AGAACTCCAG
    CCCATGTTGC AGGACTGCCC GGAGGAGATT GGCCACATGC AGCACCAACC CGATAGGAGA
    CGGCAGCCTG TGAGTCCTCC TCCACCACCA AGACCGATTT CCCCACCACA TACCTATGGC
    TACATTTCAG GACCTCTTGT CTCAGATATG GATACAGATG CACCAGAAGA GGAAGAAGAT
    GAAGCAGACA TGGAAGTGGC CAAGATGCAG ACCAGACGGC TTTTGTTACG TGGCCTTGAG
    CAGACCCCTG CCTCCAGTGT GGGGGACCTT GAAAGCTCTG TCACTGGGTC CATGATCAAT
    GGCTGGGGCT CAGCCTCAGA GGAGGACAAC ATTTCCAGTG GACGCTCCAG TGTGAGTTCT
    TCTGATGGCT CCTTCTTCAC TGATGCTGAT TTTGCCCAGG CAGTGGCAGC AGCTGCAGAA
    TATGCTGGTC TTAAAGTGGC ACGTCGTCAG ATGCAGGATG CTGCTGGTCG CCGACATTTT
    CATGCATCCC AGTGCCCTCG ACCCACAAGT CCTGTCTCTA CAGACAGCAA CATGAGTGCT
    GCTGTAATGC AGAAGGCCAG ACCAGCCAAG AAGCAAAAAC ACCAGCCAGG ACATCTGCGC
    AGAGAAGTCT ACACAGATGA TCTACCACCT CCTCCGGTGC CACCACCTGC TATAAAATCA
    CCCACTGTCC AATCCAAGGC ACAGCTGGAG GTGCGACCTG TTATGGTGCC AAAACTCCCT
    TCTATAGAAG CAAGAACAGA CCGATCATCA GATAGAAAAG TAGGCAGTTA CAAGGGGAGA
    GAAGTACTGG ATGGAAGACA GGTTGCTGAT ATGAGAACCA ATCCAGGTGA TACCAGAGAA
    GCACAGGAAC AGCAAAATGA TGGAAAAGGA CGAGTAAACA AGGCAGCAAA ACGAGACCTT
    CCACCAGCAA AGACTCACCT CATCCAAGAG GATATCCTAC CTTACTGTAG ACCTACTTTT
    CCGACATCAA ATAATCCCAG AGATCCTAGT TCTTCAAGCT CAATGTCATC AAGAGGATCA
    GGAAGCAGAC AAAGAGAACA AGCAAATGTA GGTCGAAGAA ATATCGCAGA AATGCAAGTT
    CTTGGAGGAT ATGAAAGAGG AGAAGATAAT AATGAAGAAT TAGAGGAAAC TGAAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638808.1
    CDS303..5102
    Translation

    Target ORF information:

    RefSeq Version XM_005638751.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638751.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAAATGGA AACATGTTCC TTTTTTGGTC ATAATATCAC TCCTCAGCTT GTCCAAAGAT 
    CACCTGTTCC TGGCGCAGCT TATTCCCGAC CCTGAAGACC TAGAGAGGGG GAACGACAAC
    GGGACGCCAA TCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTCCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CTTCAGACCT AATTGTGTCA
    AAAGGAGAAC CTGCAACTCT GAACTGTAAA GCTGAAGGCC GCCCCACGCC CACCATTGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTGGCACGG AATTACCTCG GAGAGGCTGT GAGCCATAAT
    GCATCGCTGG AGGTGGCTAT ACTACGGGAT GACTTCAGAC AAAATCCTTC TGATGTCATG
    GTGGCAGTGG GGGAACCTGC TGTGATGGAA TGCCAACCCC CACGAGGCCA TCCTGAACCC
    ACCATTTCGT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGTGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCTGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAACGTGAG AGTGAAGTAG CAGAGCTAAC TGTCTTGGAG
    AGACCATCAT TTGTGAAGAG ACCTAGTAAC TTGGCTGTAA CTGTGGATGA CAGTGCAGAA
    TTTAAATGTG AGGCTCGAGG TGACCCTGTG CCTACGGTAC GATGGAGGAA AGACGATGGA
    GAACTGCCCA AATCCAGATA CGAAATCAGA GATGATCATA CTTTGAAGAT TCGGAAAGTG
    ATGGCTGGTG ACATGGGGTC ATATACTTGT GTTGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCAGCTA CTCTGACTGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCT
    CGTGACCAGG TTGTTGCCTT GGGGCGAACT GTAACATTTC AGTGTGAAGC AACCGGAAAT
    CCTCAACCAG CTATTTTTTG GAGAAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCGCCACAGT CAACTAGCCG CTTTTCCGTC TCCCAGACTG GTGATCTCAC TATTACTAAT
    GTCCAGCGGT CTGATGTTGG TTATTATATC TGCCAGACTT TAAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAATGCC CACGATTCTC TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTA GAATCAAACA GCTGGAGACG GGAGTGCTGC AGATCCGATA CGCTAAGCTG
    GGTGACACAG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCACGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCAG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CAACATCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCA
    AATGCCTATG GAATTAGTGA TCCAAGCCAG ATATCAGATC CAGTGAAAAC ACAAGATGTT
    CCCCCAACAA GTCAGGGAGT AGATCACAAG CAGGTCCAGA GAGAATTGGG AAACGTCGTA
    CTGCACCTTC ACAACCCCAC CATCCTCTCA TCCTCTTCCA TCGAAGTGCA CTGGACAGTG
    GACCAACAAT CTCAGTATAT TCAAGGATAT AAAATTCTTT ATCGGCCATC TGGAGGCAAC
    CATGGTGAAT CGGAGTGGTT AGTTTTTGAA GTGAGGACTC CAACCAAAAA TAGCGTGGTT
    ATCCCTGATC TCAAAAAGGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCT TAGAAGAAGC ACCCAGTGCC
    CCACCCCAAA GTGTAACCGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT
    CTGGGCAATG AAACTCGTTA TCACATAAAC AAGACGGTGG ATGGTTCCAC CTTTTCTGTG
    GTCATTCCTT CTCTGGTTCC TGGGATCCGA TACAGCGTAG AAGTAGCAGC CAGCACGGGA
    GCTGGAGCTG GGGTGAAGAG CGAGCCACAG TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AAGACCAAGT CAGCCTTGCT CAGCAGATCT CAGATGTTGT GAAGCAGCCA
    GCGTTCATTG CGGGAATCGG GGCCGCCTGC TGGATCATCC TCATGGTCTT TAGCATCTGG
    CTCTATCGAC ACCGGAAGAA GAGAAATGGA CTGACTAGTA CTTATGCGGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGTGA AGCTGTGAGC
    AGTGGAGGGA GGCCAGGACT TCTGAACATC AGCGAACCTG CCACACAGCC CTGGCTGGCA
    GACACGTGGC CCAATACTGG CAACAATCAC AATGACTGCT CTATCAATTG CTGTACGGCA
    GGCAATGGTA ACAGTGATAA CAACCTGACC ACCTACAGTC GCCCAGGGCA GCCCACTCCT
    TATGCCACCA CCCAGCTCAT CCAGTCAAAC CTCAGCAACA ACATGAACAA TGGCAGCGGG
    GACTCCAGCG AGAAGCACTG GAAACCTCCC GGCCAGCAGA AACAAGAAGT GCCACCAGTT
    CAGTATAACA TCATGGAGCA AAACAAACTG AACAAAGATT ATCGAGCAAA TGACACAGTT
    CCTCCAACTA TCCCATACAA CCAATCATAT GACCAGAATA CAGGAGGATC CTACAACAGT
    TCAGACCGGG GCAGTAGTAC ATCTGGGAGT CAAGGGCACA AGAAAGGGGC ACGGACACCC
    AAAGTACCAA AACAAGGTGG CATGAACTGG GCAGACCTGC TTCCTCCTCC CCCAGCGCAT
    CCTCCTCCTC ATAGCAATAG TGAAGAGTAC AATATTTCTG TAGATGAAAG CTATGACCAA
    GAAATGCCAT GTCCCGTGCC ACCAGCAAGG ATGTATTTGC AACAGAATGA ACTAGAAGAG
    GAGGAAGATG AGCGAGGCCC CACTCCCCCT GTGCGGGGAG CAGCTTCTTC TCCAGCTGCT
    GTGTCTTATA GCCATCAGTC CACTGCTACT CTGACCCCCT CCCCACAAGA AGAACTCCAG
    CCCATGTTGC AGGACTGCCC GGAGGAGATT GGCCACATGC AGCACCAACC CGATAGGAGA
    CGGCAGCCTG TGAGTCCTCC TCCACCACCA AGACCGATTT CCCCACCACA TACCTATGGC
    TACATTTCAG GACCTCTTGT CTCAGATATG GATACAGATG CACCAGAAGA GGAAGAAGAT
    GAAGCAGACA TGGAAGTGGC CAAGATGCAG ACCAGACGGC TTTTGTTACG TGGCCTTGAG
    CAGACCCCTG CCTCCAGTGT GGGGGACCTT GAAAGCTCTG TCACTGGGTC CATGATCAAT
    GGCTGGGGCT CAGCCTCAGA GGAGGACAAC ATTTCCAGTG GACGCTCCAG TGTGAGTTCT
    TCTGATGGCT CCTTCTTCAC TGATGCTGAT TTTGCCCAGG CAGTGGCAGC AGCTGCAGAA
    TATGCTGGTC TTAAAGTGGC ACGTCGTCAG ATGCAGGATG CTGCTGGTCG CCGACATTTT
    CATGCATCCC AGTGCCCTCG ACCCACAAGT CCTGTCTCTA CAGACAGCAA CATGAGTGCT
    GCTGTAATGC AGAAGGCCAG ACCAGCCAAG AAGCAAAAAC ACCAGCCAGG ACATCTGCGC
    AGAGAAGTCT ACACAGATGA TCTACCACCT CCTCCGGTGC CACCACCTGC TATAAAATCA
    CCCACTGTCC AATCCAAGGC ACAGCTGGAG GTGCGACCTG TTATGGTGCC AAAACTCCCT
    TCTATAGAAG CAAGAACAGA CCGATCATCA GATAGAAAAG TAGGCAGTTA CAAGGGGAGA
    GAAGTACTGG ATGGAAGACA GGTTGCTGAT ATGAGAACCA ATCCAGGTGA TACCAGAGAA
    GCACAGGAAC AGCAAAATGA TGGAAAAGGA CGAGTAAACA AGGCAGCAAA ACGAGACCTT
    CCACCAGCAA AGACTCACCT CATCCAAGAG GATATCCTAC CTTACTGTAG ACCTACTTTT
    CCGACATCAA ATAATCCCAG AGATCCTAGT TCTTCAAGCT CAATGTCATC AAGAGGATCA
    GGAAGCAGAC AAAGAGAACA AGCAAATGTA GGTCGAAGAA ATATCGCAGA AATGCAAGTT
    CTTGGAGGAT ATGAAAGAGG AGAAGATAAT AATGAAGAAT TAGAGGAAAC TGAAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29281
    Clone ID Related Accession (Same CDS sequence) XM_005638752.2
    Accession Version XM_005638752.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4761bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product roundabout homolog 1 isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638752.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAAATGGA AACATGTTCC TTTTTTGGTC ATAATATCAC TCCTCAGCTT GTCCAAAGAT 
    CACCTGTTCC TGGCGCAGCT TATTCCCGAC CCTGAAGACC TAGAGAGGGG GAACGACAAC
    GGGACGCCAA TCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTCCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CTTCAGACCT AATTGTGTCA
    AAAGGAGAAC CTGCAACTCT GAACTGTAAA GCTGAAGGCC GCCCCACGCC CACCATTGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTGGCACGG AATTACCTCG GAGAGGCTGT GAGCCATAAT
    GCATCGCTGG AGGTGGCTAT ACTACGGGAT GACTTCAGAC AAAATCCTTC TGATGTCATG
    GTGGCAGTGG GGGAACCTGC TGTGATGGAA TGCCAACCCC CACGAGGCCA TCCTGAACCC
    ACCATTTCGT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGTGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCTGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAACGTGAG AGTGAAGTAG CAGAGCTAAC TGTCTTGGAG
    AGACCATCAT TTGTGAAGAG ACCTAGTAAC TTGGCTGTAA CTGTGGATGA CAGTGCAGAA
    TTTAAATGTG AGGCTCGAGG TGACCCTGTG CCTACGGTAC GATGGAGGAA AGACGATGGA
    GAACTGCCCA AATCCAGATA CGAAATCAGA GATGATCATA CTTTGAAGAT TCGGAAAGTG
    ATGGCTGGTG ACATGGGGTC ATATACTTGT GTTGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCAGCTA CTCTGACTGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCT
    CGTGACCAGG TTGTTGCCTT GGGGCGAACT GTAACATTTC AGTGTGAAGC AACCGGAAAT
    CCTCAACCAG CTATTTTTTG GAGAAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCGCCACAGT CAACTAGCCG CTTTTCCGTC TCCCAGACTG GTGATCTCAC TATTACTAAT
    GTCCAGCGGT CTGATGTTGG TTATTATATC TGCCAGACTT TAAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAATGCC CACGATTCTC TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTA GAATCAAACA GCTGGAGACG GGAGTGCTGC AGATCCGATA CGCTAAGCTG
    GGTGACACAG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCACGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCAG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CAACATCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCA
    AATGCCTATG GAATTAGTGA TCCAAGCCAG ATATCAGATC CAGTGAAAAC ACAAGATGTT
    CCCCCAACAA GTCAGGGAGT AGATCACAAG CAGGTCCAGA GAGAATTGGG AAACGTCGTA
    CTGCACCTTC ACAACCCCAC CATCCTCTCA TCCTCTTCCA TCGAAGTGCA CTGGACAGTG
    GACCAACAAT CTCAGTATAT TCAAGGATAT AAAATTCTTT ATCGGCCATC TGGAGGCAAC
    CATGGTGAAT CGGAGTGGTT AGTTTTTGAA GTGAGGACTC CAACCAAAAA TAGCGTGGTT
    ATCCCTGATC TCAAAAAGGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCT TAGAAGAAGC ACCCAGTGCC
    CCACCCCAAA GTGTAACCGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT
    CTGGGCAATG AAACTCGTTA TCACATAAAC AAGACGGTGG ATGGTTCCAC CTTTTCTGTG
    GTCATTCCTT CTCTGGTTCC TGGGATCCGA TACAGCGTAG AAGTAGCAGC CAGCACGGGA
    GCTGGAGCTG GGGTGAAGAG CGAGCCACAG TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AAGACCAAGT CAGCCTTGCT CAGCAGATCT CAGATGTTGT GAAGCAGCCA
    GCGTTCATTG CGGGAATCGG GGCCGCCTGC TGGATCATCC TCATGGTCTT TAGCATCTGG
    CTCTATCGAC ACCGGAAGAA GAGAAATGGA CTGACTAGTA CTTATGCGGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGTGA AGCTGTGAGC
    AGTGGAGGGA GGCCAGGACT TCTGAACATC AGCGAACCTG CCACACAGCC CTGGCTGGCA
    GACACGTGGC CCAATACTGG CAACAATCAC AATGACTGCT CTATCAATTG CTGTACGGCA
    GGCAATGGGC AGCCCACTCC TTATGCCACC ACCCAGCTCA TCCAGTCAAA CCTCAGCAAC
    AACATGAACA ATGGCAGCGG GGACTCCAGC GAGAAGCACT GGAAACCTCC CGGCCAGCAG
    AAACAAGAAG TGCCACCAGT TCAGTATAAC ATCATGGAGC AAAACAAACT GAACAAAGAT
    TATCGAGCAA ATGACACAGT TCCTCCAACT ATCCCATACA ACCAATCATA TGACCAGAAT
    ACAGGAGGAT CCTACAACAG TTCAGACCGG GGCAGTAGTA CATCTGGGAG TCAAGGGCAC
    AAGAAAGGGG CACGGACACC CAAAGTACCA AAACAAGGTG GCATGAACTG GGCAGACCTG
    CTTCCTCCTC CCCCAGCGCA TCCTCCTCCT CATAGCAATA GTGAAGAGTA CAATATTTCT
    GTAGATGAAA GCTATGACCA AGAAATGCCA TGTCCCGTGC CACCAGCAAG GATGTATTTG
    CAACAGAATG AACTAGAAGA GGAGGAAGAT GAGCGAGGCC CCACTCCCCC TGTGCGGGGA
    GCAGCTTCTT CTCCAGCTGC TGTGTCTTAT AGCCATCAGT CCACTGCTAC TCTGACCCCC
    TCCCCACAAG AAGAACTCCA GCCCATGTTG CAGGACTGCC CGGAGGAGAT TGGCCACATG
    CAGCACCAAC CCGATAGGAG ACGGCAGCCT GTGAGTCCTC CTCCACCACC AAGACCGATT
    TCCCCACCAC ATACCTATGG CTACATTTCA GGACCTCTTG TCTCAGATAT GGATACAGAT
    GCACCAGAAG AGGAAGAAGA TGAAGCAGAC ATGGAAGTGG CCAAGATGCA GACCAGACGG
    CTTTTGTTAC GTGGCCTTGA GCAGACCCCT GCCTCCAGTG TGGGGGACCT TGAAAGCTCT
    GTCACTGGGT CCATGATCAA TGGCTGGGGC TCAGCCTCAG AGGAGGACAA CATTTCCAGT
    GGACGCTCCA GTGTGAGTTC TTCTGATGGC TCCTTCTTCA CTGATGCTGA TTTTGCCCAG
    GCAGTGGCAG CAGCTGCAGA ATATGCTGGT CTTAAAGTGG CACGTCGTCA GATGCAGGAT
    GCTGCTGGTC GCCGACATTT TCATGCATCC CAGTGCCCTC GACCCACAAG TCCTGTCTCT
    ACAGACAGCA ACATGAGTGC TGCTGTAATG CAGAAGGCCA GACCAGCCAA GAAGCAAAAA
    CACCAGCCAG GACATCTGCG CAGAGAAGTC TACACAGATG ATCTACCACC TCCTCCGGTG
    CCACCACCTG CTATAAAATC ACCCACTGTC CAATCCAAGG CACAGCTGGA GGTGCGACCT
    GTTATGGTGC CAAAACTCCC TTCTATAGAA GCAAGAACAG ACCGATCATC AGATAGAAAA
    GTAGGCAGTT ACAAGGGGAG AGAAGTACTG GATGGAAGAC AGGTTGCTGA TATGAGAACC
    AATCCAGGTG ATACCAGAGA AGCACAGGAA CAGCAAAATG ATGGAAAAGG ACGAGTAAAC
    AAGGCAGCAA AACGAGACCT TCCACCAGCA AAGACTCACC TCATCCAAGA GGATATCCTA
    CCTTACTGTA GACCTACTTT TCCGACATCA AATAATCCCA GAGATCCTAG TTCTTCAAGC
    TCAATGTCAT CAAGAGGATC AGGAAGCAGA CAAAGAGAAC AAGCAAATGT AGGTCGAAGA
    AATATCGCAG AAATGCAAGT TCTTGGAGGA TATGAAAGAG GAGAAGATAA TAATGAAGAA
    TTAGAGGAAA CTGAAAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638809.1
    CDS303..5063
    Translation

    Target ORF information:

    RefSeq Version XM_005638752.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris roundabout guidance receptor 1 (ROBO1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638752.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAAATGGA AACATGTTCC TTTTTTGGTC ATAATATCAC TCCTCAGCTT GTCCAAAGAT 
    CACCTGTTCC TGGCGCAGCT TATTCCCGAC CCTGAAGACC TAGAGAGGGG GAACGACAAC
    GGGACGCCAA TCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTCCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CTTCAGACCT AATTGTGTCA
    AAAGGAGAAC CTGCAACTCT GAACTGTAAA GCTGAAGGCC GCCCCACGCC CACCATTGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTGGCACGG AATTACCTCG GAGAGGCTGT GAGCCATAAT
    GCATCGCTGG AGGTGGCTAT ACTACGGGAT GACTTCAGAC AAAATCCTTC TGATGTCATG
    GTGGCAGTGG GGGAACCTGC TGTGATGGAA TGCCAACCCC CACGAGGCCA TCCTGAACCC
    ACCATTTCGT GGAAGAAAGA TGGCTCTCCA CTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGTGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCTGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAACGTGAG AGTGAAGTAG CAGAGCTAAC TGTCTTGGAG
    AGACCATCAT TTGTGAAGAG ACCTAGTAAC TTGGCTGTAA CTGTGGATGA CAGTGCAGAA
    TTTAAATGTG AGGCTCGAGG TGACCCTGTG CCTACGGTAC GATGGAGGAA AGACGATGGA
    GAACTGCCCA AATCCAGATA CGAAATCAGA GATGATCATA CTTTGAAGAT TCGGAAAGTG
    ATGGCTGGTG ACATGGGGTC ATATACTTGT GTTGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCAGCTA CTCTGACTGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCT
    CGTGACCAGG TTGTTGCCTT GGGGCGAACT GTAACATTTC AGTGTGAAGC AACCGGAAAT
    CCTCAACCAG CTATTTTTTG GAGAAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCGCCACAGT CAACTAGCCG CTTTTCCGTC TCCCAGACTG GTGATCTCAC TATTACTAAT
    GTCCAGCGGT CTGATGTTGG TTATTATATC TGCCAGACTT TAAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAATGCC CACGATTCTC TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTA GAATCAAACA GCTGGAGACG GGAGTGCTGC AGATCCGATA CGCTAAGCTG
    GGTGACACAG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAAGC AACATGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCACGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCAG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CAACATCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCA
    AATGCCTATG GAATTAGTGA TCCAAGCCAG ATATCAGATC CAGTGAAAAC ACAAGATGTT
    CCCCCAACAA GTCAGGGAGT AGATCACAAG CAGGTCCAGA GAGAATTGGG AAACGTCGTA
    CTGCACCTTC ACAACCCCAC CATCCTCTCA TCCTCTTCCA TCGAAGTGCA CTGGACAGTG
    GACCAACAAT CTCAGTATAT TCAAGGATAT AAAATTCTTT ATCGGCCATC TGGAGGCAAC
    CATGGTGAAT CGGAGTGGTT AGTTTTTGAA GTGAGGACTC CAACCAAAAA TAGCGTGGTT
    ATCCCTGATC TCAAAAAGGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCT TAGAAGAAGC ACCCAGTGCC
    CCACCCCAAA GTGTAACCGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCAGAAGA CACTCAAAAT GGAATGGTCC AAGAGTATAA GGTTTGGTGT
    CTGGGCAATG AAACTCGTTA TCACATAAAC AAGACGGTGG ATGGTTCCAC CTTTTCTGTG
    GTCATTCCTT CTCTGGTTCC TGGGATCCGA TACAGCGTAG AAGTAGCAGC CAGCACGGGA
    GCTGGAGCTG GGGTGAAGAG CGAGCCACAG TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AAGACCAAGT CAGCCTTGCT CAGCAGATCT CAGATGTTGT GAAGCAGCCA
    GCGTTCATTG CGGGAATCGG GGCCGCCTGC TGGATCATCC TCATGGTCTT TAGCATCTGG
    CTCTATCGAC ACCGGAAGAA GAGAAATGGA CTGACTAGTA CTTATGCGGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGTGA AGCTGTGAGC
    AGTGGAGGGA GGCCAGGACT TCTGAACATC AGCGAACCTG CCACACAGCC CTGGCTGGCA
    GACACGTGGC CCAATACTGG CAACAATCAC AATGACTGCT CTATCAATTG CTGTACGGCA
    GGCAATGGGC AGCCCACTCC TTATGCCACC ACCCAGCTCA TCCAGTCAAA CCTCAGCAAC
    AACATGAACA ATGGCAGCGG GGACTCCAGC GAGAAGCACT GGAAACCTCC CGGCCAGCAG
    AAACAAGAAG TGCCACCAGT TCAGTATAAC ATCATGGAGC AAAACAAACT GAACAAAGAT
    TATCGAGCAA ATGACACAGT TCCTCCAACT ATCCCATACA ACCAATCATA TGACCAGAAT
    ACAGGAGGAT CCTACAACAG TTCAGACCGG GGCAGTAGTA CATCTGGGAG TCAAGGGCAC
    AAGAAAGGGG CACGGACACC CAAAGTACCA AAACAAGGTG GCATGAACTG GGCAGACCTG
    CTTCCTCCTC CCCCAGCGCA TCCTCCTCCT CATAGCAATA GTGAAGAGTA CAATATTTCT
    GTAGATGAAA GCTATGACCA AGAAATGCCA TGTCCCGTGC CACCAGCAAG GATGTATTTG
    CAACAGAATG AACTAGAAGA GGAGGAAGAT GAGCGAGGCC CCACTCCCCC TGTGCGGGGA
    GCAGCTTCTT CTCCAGCTGC TGTGTCTTAT AGCCATCAGT CCACTGCTAC TCTGACCCCC
    TCCCCACAAG AAGAACTCCA GCCCATGTTG CAGGACTGCC CGGAGGAGAT TGGCCACATG
    CAGCACCAAC CCGATAGGAG ACGGCAGCCT GTGAGTCCTC CTCCACCACC AAGACCGATT
    TCCCCACCAC ATACCTATGG CTACATTTCA GGACCTCTTG TCTCAGATAT GGATACAGAT
    GCACCAGAAG AGGAAGAAGA TGAAGCAGAC ATGGAAGTGG CCAAGATGCA GACCAGACGG
    CTTTTGTTAC GTGGCCTTGA GCAGACCCCT GCCTCCAGTG TGGGGGACCT TGAAAGCTCT
    GTCACTGGGT CCATGATCAA TGGCTGGGGC TCAGCCTCAG AGGAGGACAA CATTTCCAGT
    GGACGCTCCA GTGTGAGTTC TTCTGATGGC TCCTTCTTCA CTGATGCTGA TTTTGCCCAG
    GCAGTGGCAG CAGCTGCAGA ATATGCTGGT CTTAAAGTGG CACGTCGTCA GATGCAGGAT
    GCTGCTGGTC GCCGACATTT TCATGCATCC CAGTGCCCTC GACCCACAAG TCCTGTCTCT
    ACAGACAGCA ACATGAGTGC TGCTGTAATG CAGAAGGCCA GACCAGCCAA GAAGCAAAAA
    CACCAGCCAG GACATCTGCG CAGAGAAGTC TACACAGATG ATCTACCACC TCCTCCGGTG
    CCACCACCTG CTATAAAATC ACCCACTGTC CAATCCAAGG CACAGCTGGA GGTGCGACCT
    GTTATGGTGC CAAAACTCCC TTCTATAGAA GCAAGAACAG ACCGATCATC AGATAGAAAA
    GTAGGCAGTT ACAAGGGGAG AGAAGTACTG GATGGAAGAC AGGTTGCTGA TATGAGAACC
    AATCCAGGTG ATACCAGAGA AGCACAGGAA CAGCAAAATG ATGGAAAAGG ACGAGTAAAC
    AAGGCAGCAA AACGAGACCT TCCACCAGCA AAGACTCACC TCATCCAAGA GGATATCCTA
    CCTTACTGTA GACCTACTTT TCCGACATCA AATAATCCCA GAGATCCTAG TTCTTCAAGC
    TCAATGTCAT CAAGAGGATC AGGAAGCAGA CAAAGAGAAC AAGCAAATGT AGGTCGAAGA
    AATATCGCAG AAATGCAAGT TCTTGGAGGA TATGAAAGAG GAGAAGATAA TAATGAAGAA
    TTAGAGGAAA CTGAAAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.